LOCUS       DAXWLH010000001        55041 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXWLH010000001 DAXWLH010000000
VERSION     DAXWLH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.001"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE36_000001"
     CDS             complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE36_000001"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8382435.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHI"
     gene            complement(1868..2245)
                     /locus_tag="ACGE36_000002"
     CDS             complement(1868..2245)
                     /locus_tag="ACGE36_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382436.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            2501..3229
                     /gene="purC"
                     /locus_tag="ACGE36_000003"
     CDS             2501..3229
                     /gene="purC"
                     /locus_tag="ACGE36_000003"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFF8382437.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            3229..3483
                     /gene="purS"
                     /locus_tag="ACGE36_000004"
     CDS             3229..3483
                     /gene="purS"
                     /locus_tag="ACGE36_000004"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFF8382438.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            3485..4156
                     /gene="purQ"
                     /locus_tag="ACGE36_000005"
     CDS             3485..4156
                     /gene="purQ"
                     /locus_tag="ACGE36_000005"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFF8382439.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            4157..6379
                     /gene="purL"
                     /locus_tag="ACGE36_000006"
     CDS             4157..6379
                     /gene="purL"
                     /locus_tag="ACGE36_000006"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFF8382440.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            6364..7803
                     /gene="purF"
                     /locus_tag="ACGE36_000007"
     CDS             6364..7803
                     /gene="purF"
                     /locus_tag="ACGE36_000007"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFF8382441.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            7826..8878
                     /gene="purM"
                     /locus_tag="ACGE36_000008"
     CDS             7826..8878
                     /gene="purM"
                     /locus_tag="ACGE36_000008"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFF8382442.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            8875..9453
                     /gene="purN"
                     /locus_tag="ACGE36_000009"
     CDS             8875..9453
                     /gene="purN"
                     /locus_tag="ACGE36_000009"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFF8382443.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            9450..10985
                     /gene="purH"
                     /locus_tag="ACGE36_000010"
     CDS             9450..10985
                     /gene="purH"
                     /locus_tag="ACGE36_000010"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFF8382444.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            11001..12275
                     /gene="purD"
                     /locus_tag="ACGE36_000011"
     CDS             11001..12275
                     /gene="purD"
                     /locus_tag="ACGE36_000011"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFF8382445.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            12397..13311
                     /locus_tag="ACGE36_000012"
     CDS             12397..13311
                     /locus_tag="ACGE36_000012"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFF8382446.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(13444..15009)
                     /locus_tag="ACGE36_000013"
     CDS             complement(13444..15009)
                     /locus_tag="ACGE36_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8382447.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(15002..16453)
                     /locus_tag="ACGE36_000014"
     CDS             complement(15002..16453)
                     /locus_tag="ACGE36_000014"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8382448.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(16467..17483)
                     /locus_tag="ACGE36_000015"
     CDS             complement(16467..17483)
                     /locus_tag="ACGE36_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8382449.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            17703..18791
                     /gene="chvE"
                     /locus_tag="ACGE36_000016"
     CDS             17703..18791
                     /gene="chvE"
                     /locus_tag="ACGE36_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8382450.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            18845..20371
                     /gene="mmsA"
                     /locus_tag="ACGE36_000017"
     CDS             18845..20371
                     /gene="mmsA"
                     /locus_tag="ACGE36_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8382451.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            20373..21578
                     /gene="mmsB"
                     /locus_tag="ACGE36_000018"
     CDS             20373..21578
                     /gene="mmsB"
                     /locus_tag="ACGE36_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFF8382452.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAVRLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            21835..22812
                     /gene="guaC"
                     /locus_tag="ACGE36_000019"
     CDS             21835..22812
                     /gene="guaC"
                     /locus_tag="ACGE36_000019"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFF8382453.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            23076..24500
                     /locus_tag="ACGE36_000020"
     CDS             23076..24500
                     /locus_tag="ACGE36_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8382454.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(24542..24799)
                     /locus_tag="ACGE36_000021"
     CDS             complement(24542..24799)
                     /locus_tag="ACGE36_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382455.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            24961..26070
                     /gene="pdhA"
                     /locus_tag="ACGE36_000022"
     CDS             24961..26070
                     /gene="pdhA"
                     /locus_tag="ACGE36_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFF8382456.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            26073..27050
                     /locus_tag="ACGE36_000023"
     CDS             26073..27050
                     /locus_tag="ACGE36_000023"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFF8382457.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            27075..28718
                     /locus_tag="ACGE36_000024"
     CDS             27075..28718
                     /locus_tag="ACGE36_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFF8382458.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEIQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            28726..30132
                     /gene="lpdA"
                     /locus_tag="ACGE36_000025"
     CDS             28726..30132
                     /gene="lpdA"
                     /locus_tag="ACGE36_000025"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8382459.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            30294..30569
                     /locus_tag="ACGE36_000026"
     CDS             30294..30569
                     /locus_tag="ACGE36_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFF8382460.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            30575..31342
                     /locus_tag="ACGE36_000027"
     CDS             30575..31342
                     /locus_tag="ACGE36_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFF8382461.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            31510..33339
                     /gene="typA"
                     /locus_tag="ACGE36_000028"
     CDS             31510..33339
                     /gene="typA"
                     /locus_tag="ACGE36_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFF8382462.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            33600..35372
                     /gene="recQ"
                     /locus_tag="ACGE36_000029"
     CDS             33600..35372
                     /gene="recQ"
                     /locus_tag="ACGE36_000029"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFF8382463.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            35486..35788
                     /locus_tag="ACGE36_000030"
     CDS             35486..35788
                     /locus_tag="ACGE36_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFF8382464.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            35791..36954
                     /locus_tag="ACGE36_000031"
     CDS             35791..36954
                     /locus_tag="ACGE36_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8382465.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            36980..40408
                     /locus_tag="ACGE36_000032"
     CDS             36980..40408
                     /locus_tag="ACGE36_000032"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFF8382466.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            40429..41553
                     /locus_tag="ACGE36_000033"
     CDS             40429..41553
                     /locus_tag="ACGE36_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFF8382467.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            41534..41980
                     /locus_tag="ACGE36_000034"
     CDS             41534..41980
                     /locus_tag="ACGE36_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8382468.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            41964..42302
                     /locus_tag="ACGE36_000035"
     CDS             41964..42302
                     /locus_tag="ACGE36_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFF8382469.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            42421..42981
                     /gene="rsmD"
                     /locus_tag="ACGE36_000036"
     CDS             42421..42981
                     /gene="rsmD"
                     /locus_tag="ACGE36_000036"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFF8382470.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTIDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            42978..43469
                     /gene="coaD"
                     /locus_tag="ACGE36_000037"
     CDS             42978..43469
                     /gene="coaD"
                     /locus_tag="ACGE36_000037"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFF8382471.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            43459..44502
                     /locus_tag="ACGE36_000038"
     CDS             43459..44502
                     /locus_tag="ACGE36_000038"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFF8382472.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            44661..45389
                     /locus_tag="ACGE36_000039"
     CDS             44661..45389
                     /locus_tag="ACGE36_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8382473.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            45505..45750
                     /locus_tag="ACGE36_000040"
     CDS             45505..45750
                     /locus_tag="ACGE36_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8382474.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            45747..45965
                     /locus_tag="ACGE36_000041"
     CDS             45747..45965
                     /locus_tag="ACGE36_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFF8382475.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            46101..46640
                     /locus_tag="ACGE36_000042"
     CDS             46101..46640
                     /locus_tag="ACGE36_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFF8382476.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            46633..48906
                     /locus_tag="ACGE36_000043"
     CDS             46633..48906
                     /locus_tag="ACGE36_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFF8382477.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            48944..49990
                     /gene="holA"
                     /locus_tag="ACGE36_000044"
     CDS             48944..49990
                     /gene="holA"
                     /locus_tag="ACGE36_000044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFF8382478.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(50079..50333)
                     /gene="rpsT"
                     /locus_tag="ACGE36_000045"
     CDS             complement(50079..50333)
                     /gene="rpsT"
                     /locus_tag="ACGE36_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFF8382479.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(50733..51002)
                     /locus_tag="ACGE36_000046"
     CDS             complement(50733..51002)
                     /locus_tag="ACGE36_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382480.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(51264..51392)
                     /locus_tag="ACGE36_000047"
     CDS             complement(51264..51392)
                     /locus_tag="ACGE36_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382481.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(51472..51927)
                     /locus_tag="ACGE36_000048"
     CDS             complement(51472..51927)
                     /locus_tag="ACGE36_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFF8382482.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(52065..53993)
                     /locus_tag="ACGE36_000049"
     CDS             complement(52065..53993)
                     /locus_tag="ACGE36_000049"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8382483.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(54190..55023)
                     /locus_tag="ACGE36_000050"
     CDS             complement(54190..55023)
                     /locus_tag="ACGE36_000050"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFF8382484.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT        17930 a         9600 c        11900 g        15611 t
ORIGIN      
        1 aatatgactt ttggagactg accaaatacc ttttcttgta tttgaccatt catacgcttt
       61 cgctttatcg atccctaaac gaatcagatt ttttcttttg gttcttggat tcttccattg
      121 tttccaaata tattgtctga ttcttcgctt caaccaaccg tttaactccc tcatgaattc
      181 tttcatctca ccaattccat agtagttgat ccaaccagtc attagttgct tgatttcttt
      241 taaaataagt tggatacttc ttccacggtt tcttttagtg agttgtttga gcttgctttt
      301 cacacgctgt tttgcttttg cgtgagggcg aattttaata cctttctttg ttggcagaat
      361 gcagaatcca agaaactttc gtttcaatgg acttcctacc gcactctttt ctcgatttac
      421 cgtcaacttt agttgttctt cgagaaattg aatggatttc ttcatcacac gctgtcctgc
      481 tcgtttgctt ctaacataga tattgcagtc atcggcgtat cgaacaaatt tatgtcctag
      541 attttctagc acttgatcaa attcgttcag gtagatgtta cttaacaggg gagagagatt
      601 gcctccttgg ggcgttcctt cttcagttgt tgtgatgagt ccattttcca ttacaccact
      661 cattaagtat tgacggatca gttttaggac ttgtttgtcc tttactttcc gctcaatgaa
      721 atacatcagc ttatcgtgat ttacggtgtc aaagtaggct ttcatatcaa tgtccaccac
      781 atatcggtaa ccttgttcat aataagattt tgctttcttc actgcgtcat gtgcgctgcg
      841 atttgggcga aacccataac tattatctga gaattctggt tcaaatagcg gttgtaacac
      901 ttgattgatt gcctgttgaa gcatacgatc aataaccgtg ggaattccta gttttcttac
      961 accgccgttt ggtttcggta tttctactcg caaaacaggc tgaggtttat agttcccttg
     1021 gcgtaaattg gatagtaatt cctcgcggtg ctctttcaag taaggaagca gttgatcgac
     1081 ggtcatccca tctgcaccag ccgccccttt atttctgact acttgaagat acgcttgatt
     1141 gagattattc cgatctagaa ttctcccaaa cagattttct acaccatctt gtttcctctg
     1201 ttcaccgcaa gaagtgctgc gcacttcatc atacccttcg tgttccacac tagctctttg
     1261 atgatagcca agttttcttg ttttctgcgg tcgtcgcatc ttctcacacc tcctaaatta
     1321 ttcaagattc ttattgttca gtccttcgta acggtcagtg tcgttactac tatgacctcg
     1381 gctgacttct catgattcac catcatgtcg ccatgatcgt tgtgctggtt tgttttcatt
     1441 caacgcccac aacaccctca tgagatctcc cccggtaaga gtgaaaactt tcctcccatg
     1501 taactgctag atttactgta taggtttcgg gcagtattgg actttgtctt gtgatgaaga
     1561 cttatccgac ctaattcagc cttgtatcta gtttctgttc gtcagttcag gagtttgcgt
     1621 ccgacttcct tcagccacaa cctcacgatt gccgccttgt ctttcgctaa cagttcctac
     1681 tgccaagcct gtagtggact ttcaccacca agttttcacc catgcagggc gcacaccaaa
     1741 aaagaagcag atccgttagc ggttctgctt cttttagttt attatatttt cttatcaata
     1801 ataggaacct gattttaaaa gcggctacct ctttaccgtg cctctttaca tacaaaaaat
     1861 agaggaatta agcgtaaatg gcttggctat tataactaaa atgctggtta tggagatgtt
     1921 gcagcaagtt ttcgagaaaa gcgtattcca tctcttgtcc attgaataag atctggatac
     1981 gataatgatt tttgaaacgc ttctgttcat gaatctgact gattgaaagt tcgcctttgt
     2041 gttctgtctg ataaagaaaa gtccttttac ggcattcagc acagcgcttg gttgctcggc
     2101 ggatttttcg gaatgtataa aaactagtat ctttattgca taaataaacg tctttgatcg
     2161 acagcaaatc attatataga caggaacgtt cttcagctgt taattctgat aaaaaaagct
     2221 gaccttgttt tattgcatct tccatcggca agtgcctcct cgaacccatt atccaaattt
     2281 ttagttaatt ctaatatacc atcattaagt ataaatgtaa acgcttgttg atggtataag
     2341 attttcgttt ctgataaaac acgaacatta aaagaatcta atgccttaaa tgtacatatt
     2401 tttgttgatt tataaaaaga tgattgctaa aatttaaagt gaaaaaacga acaaaaaacg
     2461 atgtatttgc ttttaatgta aataaaaagg agagacgtac atggagaaga aagaactttt
     2521 gtatgaagga aaagccaaaa aaatatatct tacttctgat gagaacttga tttggataga
     2581 atatttagat caagcaactg cgttgaatgg cgcgaggaaa gaccagataa gtggaaaagg
     2641 agaattgaat aatcaaatca caagtgcaat tttccgttat ctgactcaaa gaggaataaa
     2701 aaatcatttt attgaacaat tgtctaaaac cgaacaatta gcaaaaaaag tacaaattat
     2761 ccctctggaa gtggttatcc gaaatattgc agcaggaagt ttctcaaaac gtctaggtgt
     2821 tcccgaaggt aaagaattca tcaaaccaat cattgaattc tactataagg atgaccagtt
     2881 ggatgatcca tttatcaatg aagagcatat ctatttctta caacttgcaa agccggaaga
     2941 aatcagtgag ctgaaagccc aagcgttggc tattaatgaa attttgaccg aattatttgc
     3001 taaggtcggt ttggttttag tagatttcaa gctagaattt ggccggtcga atggacaaat
     3061 tttgcttgcg gatgaagtaa caccggatac ttgccgatta tgggatgaac gtacgaatga
     3121 acatatggac aaagacgttt accgcagaga tctaggtgat ttgatcccag tctatcaaga
     3181 agtatataac cgtctatcgg aactaactga agaaaaggag cgagtatgat gtatttcgta
     3241 aaagtatatg taacctataa agattcagtg ttagatcctc aaggggaggc agtaaaaaaa
     3301 gcagtccatc gtatgggata tgaaacaatc gaagatatcc gaataggaaa atactttgag
     3361 atacaacttg ctgatgcaga agaaccaatc gatacagtga ttgaaacgat ttgtgacaag
     3421 ctgcttgcaa atgtaaatat ggaaacatac cgatatgaaa tcaaaaaaat ggaggaagta
     3481 taacatgaaa tttgcggtca tcgtttttcc aggttcaaat tgtgacgcag atatgttatg
     3541 ggcagttcgc gagatcatgg gcgctgatgc cgaatttgta cggcatgatg agtcctcttt
     3601 agaaggattt gatggggttt tactgccggg aggattctcc tatggtgact atttgagatg
     3661 cggtgcgatt gctcggtttt ctacaatcat ggaagaagtg attcgttttg ctgaagaagg
     3721 gaaaccagta ttcggtacat gcaatggatt ccagatattg acagaagcag gacttttgcc
     3781 aggtgtactg agacgaaatg agtctttgca ttttatttgc aaaactgtac cattgaaagt
     3841 caaccccaag acggccttta cttcagagta tgaagccgac gaaataatct atcttccagt
     3901 tgctcatggg gaaggaaatt atttctgtga cgaagaaaca cttcaagaac taaaaaaaaa
     3961 taatcagatt gcctttactt ataatgacaa catcaacggc agcgtggaaa acattgcagg
     4021 gattacgaac aaagcaggga atgtgttagg aatgatgcct catccagaac gtgcagtaga
     4081 agacttgcta ggatcagaag atgggaaacg gttctttgct tccattttaa aaaacttcgg
     4141 aaaggtgacc gtatagatga aaacattgaa agaaccgaca gctgaagaga tcaagaaaaa
     4201 aagaatctat gccgaatggg gattgactga tgaagaatat cgaatgatcg aagaagatat
     4261 cttaggaaga atgcctaatt atacggaaac tggactgttt tctgttatgt ggagcgaaca
     4321 ttgttcctat aaaaattcta aaccggttct acgcaaattt cctactagtg gacctcatgt
     4381 gttgcaaggc cctggagaag gcgcaggcat cgtcgacatc ggtgatggac tagcagttgt
     4441 cttcaaagca gaaagtcaca atcatccttc agccgtggag ccttatgaag gagctgcgac
     4501 aggagttggc ggaatcatca gagatatttt cagcatgggg gctcgtccaa ttgcgttgct
     4561 agattcattg aggttcggcg aattagataa tccaagaacg aaatatttac tagaagaagt
     4621 agttgcaggt atcggcggat acggcaattg tatcggcata ccgactgtag gcggcgaaat
     4681 tgcatttgat ccttgttatg aaggaaaccc attagtcaat gcaatgtgcg taggtttgat
     4741 tgaacacaaa catatccaga aagggcaagc cgctggcgtc ggtaattcga tcatgtatgt
     4801 aggtgcaaaa acaggtcgcg acggtatcca cggagcaact tttgcctcag aagaatttaa
     4861 tcaagaagaa gaacagcaac gttctgctgt acaagtaggc gatcctttta tggaaaagct
     4921 gctgcttgaa gcgtgtttgg aattaatctt gcatcattcg gattctctaa taggtataca
     4981 ggatatggga gcagcaggac ttgtttcatc tagtgcggaa atggcttcaa aagctggctc
     5041 aggacttata ttgacattag atgaagtccc acaaagagaa actgggatga caccttatga
     5101 aatgatgtta tcggaatcac aagaacggat gttgatttgt gttaaatcag gagaggaaga
     5161 aaaaatccaa gaactatttc agaagtatga tctagatgca gtcactatcg gtaaagtaac
     5221 agacgacggg caatatcggc tatatcatca aggagaagaa gtagcaaatc tgccagtcga
     5281 tgcattagct gaagatgcac cagtttatca taaagaaatg aaagaaccag ctcgaatcgt
     5341 agaatttcag cagttagctc cttatcagcc agttatcgaa gaacctggac aagtattgct
     5401 tgatctgctg cagcaaccga cgattgcttc aaaacgttca atctacgaaa cttatgattc
     5461 acaagtacaa accaatacag ttgtccgtcc aggaagcgat gctgctgtga tgagggttag
     5521 agggacgaat aaagcgttag ctatgaccac agactgcaac gcgcgctatc tgtatttgga
     5581 tccaaaaatc ggaggacaaa tagcagtggc agaagctgct agaaatatta ttgctagcgg
     5641 tggtaagccc ttggcgatca ccgactgcct gaactatggt tctcctgata aaccagaagt
     5701 tttttgggaa ttatcgactt cagctgacgg aattgctgct gcgtgtgaaa cgttaggaac
     5761 tcctgttatt tcaggaaatg tctctttata taatgaaacg gatggtcaag caatctatcc
     5821 aacaccaatg attggtatgg tcggattgat tgaggatcat aaacatatca ctactcagga
     5881 attcaaaaaa tcaggggatc taatctatat attaggtaag acgtttgcag attttgatgg
     5941 cagtgaatta caaaagatgc agcttggaag aatcgaaggt gtgatccgaa actttgatct
     6001 atccattgaa aagagaaacc aagagttagt attgacagcc atccaaaacg gattgattga
     6061 aagtgctcat gactgttcag aaggtggctt ggcaattgca ctggcagaat cagctttcaa
     6121 acatcagcta gggatatctg tacagtttga attgtcctct gctcaattat ttgcagaaac
     6181 tcaatcaaga tttgtgttga cagtcgcacc ggaaaataag acacgattcg aagaaatgat
     6241 gggagatgct gctgttttag caggtaaggt gacggatgaa gcgataatcg aaatttcagc
     6301 aactgacggt cagatcaaaa tagaaactgc agtagcaaga aagtgctggg aggatgcaat
     6361 cgtatgtctt atgaagtgaa gagtttaaat gaagaatgcg ggatcttcgg tgtttggggt
     6421 catccagacg ccgctagtgt ggcatacttc gggctccata gtcttcagca tcgaggacaa
     6481 gaaggtgcag gtatcgtgtc caatgatcga ggaaagctta aggggcatcg agatttgggg
     6541 ctgctttctg aggtattcaa agatcaaaga aaattgcagc agttaacagg agaagctgca
     6601 atcggacatg tacgttatgc aacagcaggc aatggaagtg tggacaatat tcagcctttt
     6661 ctattcaaat ttcatgatca gtcgatcggc ttagcgcata atggtaactt gatcaatgct
     6721 cgttccctta gaaaagagtt agaggaaaat ggggcgatct tccattctaa ctccgatacg
     6781 gagatattga tgcatttgat ccgacgaagt gaagaaacag attttttaaa taaattaaaa
     6841 agtgctttga atgaagtcaa aggtggcttt gcctatttat taatgacaga aacggcaatg
     6901 attgccgctt tggacccaaa tggttttcgt ccattagcaa tcggacaaat gaaaaacgga
     6961 gcatacgtga ttgcctcgga aacttgtgca ttagaagtta ttggggcacg ttttataaga
     7021 gatgtccagc ctggtgagat agtgatcatc aatgacgaag gaatccagat tgattgcttt
     7081 acgaaggaca cgcagctttc tatttgttcg atggaatata tctattttgc cagaccagat
     7141 tcaaacatcg cagggatcaa tgtgcatact gcacggaaaa atatgggacg gaatttagcg
     7201 aaagaatcgc cagtaaaagc agatatggtc gttggcgtac ctaattcttc tttgtcagcc
     7261 gcaagcggtt atgcggaagc tagtggaatc ccttatgaaa tcggattagt aaaaaatcaa
     7321 tacattgccc gaacatttat ccagccaacg cctgagctaa gagagcaagg tgttcgaatg
     7381 aaattatctg ctgtacgagg agtagtggaa ggtaaaaagg tgattatggt cgatgattca
     7441 attgttcggg gtacgacaag cagacggatc gtccatcttt tgaaagaagc aggggcaaaa
     7501 gaagtccatg tgaggattgc ttctcctcct ttgaaatatc catgttttta cgggattgat
     7561 attcaaacgc gtaaagaact cattgcggca aatcacacga ttgatgaaat caaagaatgt
     7621 atcggtgcag attctttagg ttttttaagt gaagagggtc tgatcgaatc gatcggtctg
     7681 gaaaaagatg ctccgtattc aggattgtgt atggcttatt tcaacggaga ttatccgacg
     7741 ccgttatatg actatgaaga aaaatatcag gaatcactaa aagaaaaagt atcgttcttt
     7801 taaaagcaga ccaagaaggg agtatatgaa ttcaatggca aatgcatacg caaaagctgg
     7861 cgtagatgtc gaagctggat atgaagtagt agaacggatc aagaagcatg tcaaaaaaac
     7921 ggaacgttta ggtatgatgg gcgctatagg aggattcggc ggttgttttg atttatcggt
     7981 ctttcaatta aaagagccag tccttgtctc ggggaccgat ggcgtgggga cgaaattagc
     8041 agtggcaatc gaagcagaca aacacgatac aatcgggatc gactgcgtag cgatgtgtgt
     8101 caatgatatc ttagcacaag gcgcagagcc gttatatttc ttagactact tagcaacggg
     8161 gaaaaatgat ccgttacgtt tggaaaatgt agtaaaaggt gtagcagatg gatgtgttca
     8221 aggaaatatg gcgctgattg gcggagaaac agctgaaatg cctggtatgt actcaggtaa
     8281 tgattatgat ctagccggat ttgctgtagg gatagctgaa aaatctcaat tattgacagg
     8341 agaaacgatc aacccaggag atgtgttgct tggaatagca tctagcggga ttcattcaaa
     8401 tgggtactct cttgttcgca aaattttctt tgagcagcag cagttttctt tatcatcaaa
     8461 actgccagaa ttgaaaaaaa cacttggcga agaattgctt gagccaacga aaatctatgt
     8521 ttcttccttg cttccgctga tcaaagaaaa gaaaataaaa ggagctgcac atatcactgg
     8581 tggagggttt gtagaaaata tcccgcgaat gttaccagaa tctatggctg cacaaatcaa
     8641 acttggctca tggccgattc ttccaatatt cggctgttta caaaaatatg gtgaaattcc
     8701 tgagatggag atgtatgaaa tcttcaatat gggcatcggt atggtcctgg cagtccaaga
     8761 atcgaaagta aaagaaatag tacacgaaat tgagcaagct ggcgaaaaag tttatgaaat
     8821 cgggaaagtg atcgccaaag aaaaacatcg tgtggtgttt ataggagaag aacgatgaga
     8881 atcgctgttt ttgcttctgg aaacggaagc aactttcaag cgctagccga ttatctatcg
     8941 aaaaaaggat tggaatcttc gattgactgg ttgttttgtg atcagcctga agcatatgtt
     9001 ttgaaacgtg caactgcgtt gagtgtaccg gcagattgtt tttcacctaa agaatttgat
     9061 tcaaaaaaag agtatgaaga agcaattttg cataaattga aagaaaaaaa gatcgatttg
     9121 atcgtattag ctggatatat gcgaatcatt ggaccggtat tgctgaaaaa ttatgataaa
     9181 agaatcatca atatccatcc gtctctttta cctgccttcc ctggtttgca tggcatacgt
     9241 gatgcctttg aagctggagt aaaagaaacg ggtgtcacaa ttcattatat cgaccaaggt
     9301 gtagatactg gaccaatcat ccggcaggaa aaagtgagaa ttgaacagga ggacacgttc
     9361 gattctttag aagaaaaaat ccatagagta gagcatcgaa tctatccaga agtgattagt
     9421 gaaattattg aaaatggagg ctatctgaaa tgacaagagc attgatcagt gtatcagata
     9481 aaactgggat tgtagcattc gcccaaggtt tgattgaaca aggagtagaa atcatttcaa
     9541 caggaggaac aaagcatgcg ttggataaag ctgggatcaa aacgctttcg atcgaagaag
     9601 tgacagggtt tcctgaaatg atggatggcc gtgtcaaaac acttcatcct aaaatccatg
     9661 gtggtctttt aggaagacgc gatctcccta gccatatgga ggcaatggaa acggaaaata
     9721 tccaaccaat cgactttgtt tgtgtcaatt tatatccttt caaagaaacg attatgaaac
     9781 cagatgtcga aatagcagaa gctattgaaa acattgatat cggtggtcct agtatgctcc
     9841 gtagtgcagc aaaaaaccat gaatttgtca cctctgttgt tgatccatct gactatgaag
     9901 aagtactttc agaattacgt cagtatgggc atacttcact ttctttcaga caaaaactag
     9961 cggcaaaagt ctttcgtcat actgcggcat atgatgcact gatcgctcaa tacttaacgg
    10021 attgggtgga agaaaaagaa ccggaaaaat tgacacttac gtatgaacga aaacaaacgc
    10081 ttcgttacgg tgaaaatagc catcaagccg ctgcctttta tcagtccgct cttctggaaa
    10141 aatattcgat cgcagcagca aaacaactcc atgggaaaga actttcttat aataatatcc
    10201 gagatgcaga tgctgcgcta cggatcatga aggaatatga cgagccaacg gtagtcgcct
    10261 tgaaacacat gaatccttgt ggtattggga cagcatctac gattttagaa gcgtgggaag
    10321 cagcgtatga agcagatcct gtttcaatct ttggtgggat catcgttttg aatcgagaag
    10381 tagatctgcc aacagcagaa cagatgcata agctgtttct agaaatcatc attgcccctt
    10441 cttatacaca agaagctttg gaattactgc aaaaaaagaa aaacatccga ttactgacag
    10501 tagactttgc gcaggaagaa aatcaaaatc gagaagaaac ggtctcagtc ttaggtggac
    10561 ttcttgtaca agatcaagat ctggcaatag aaaatgaaga agagtggaaa gtcgtgacaa
    10621 aacgtaagcc gactccagca gaagcaaaag cattagcgtt tgcttggaga gctgtcaaac
    10681 atgtgaaatc taatgcgatc gtactggcaa acgaacgtca gacagtagga atcggagccg
    10741 ggcagatgaa tcgagtcggt tcagtaaaaa ttgcgattga acaagcgcaa gagaaaatgg
    10801 aaggtgcagt tttagctagc gatgcttatt ttccaatgga tgattcggtt gaatatgcag
    10861 ccaaacatgg tataaaagca atcattcagc caggaggaag catcaaagac caagattcta
    10921 ttgatatggc gaataaatac gatctagcaa tggtctttac tgatgttcgg cattttagac
    10981 attagagagg agtcggataa atgaacattt tggtgatcgg tgcaggtggt cgtgaacatg
    11041 caataagtaa aaaactttta gccagtccga aggtggatac ggtgtattgt gcgccaggaa
    11101 atccggggat gacaaaagat ggtatcaatt gtgtcgctat cagtgagcga gatcattcag
    11161 cgttaatcca ttttgttaag aaagaggcta ttgattggag tatcgttggt cctgaagttc
    11221 ctttgttaaa tgggatcgtc gatgatttta tggcggaagg attgaaaatc ttcggtccga
    11281 caaaagcagc agctctgatc gaaggttcga aaagttttgc taaacaatta atggatactt
    11341 atcaaatccc gacagctaaa tccagaagtt ttgaatgtta cgaaaaagca gcagcgtata
    11401 tcaaagaaaa tggtgcgcca atcgtagtga aagcggacgg cttagcagct gggaaaggtg
    11461 tagtcgtggc taccacagtg aaggaagcgc tggatgcggc agaacaaatg ttgaaccagc
    11521 atcgatttgg ggatagtagc aaaaaaatcg tcgtagaaga gtttttggca ggagaagaat
    11581 tctccctttt ggcgtttgtc cgtaatacca aggtgtatcc gatggtcatt tcgcaagatc
    11641 ataagcgggc atttgataac gataaagggc caaatacagg cggaatgggt gcttattcac
    11701 cagtaccgca aatttcaaaa aagatgattg aaaaagctgt ggaagaagtt ttaaaaccag
    11761 cggctttggg aatgaaagaa ctaggacgct ctttcacggg tgttttatat gcgggtttga
    11821 ttgctacgaa ggaaggacca aaagtgatcg aattcaatgc gcgtttcggt gatcctgaaa
    11881 cgcaagtcgt tctttctcgg ttgaagagtg actttgcgca agtaatcgat gatttattag
    11941 agaatcgtac agtagagtta aagtggcagc aagaaggata caatgtaggc gttgtggttg
    12001 cagctgccgg ttaccccgaa gaatatgaga caggaatggt tctaccagat tttctggaag
    12061 aagagctttc tgtttactat gctggagttt cctcaaataa cggcaaacta gttggagcag
    12121 gaggacgtct ttatttagta gaagcatacg gtgagacgat caaagaagca cagaaaaaaa
    12181 tttacaccgc gttaatggat aaaacgacag atggaacatt ttatcgtaca gatattggag
    12241 aaaaagctgc caatagcgaa ctgatcgtga agtaaatcag ctgcttccat ttataaaaaa
    12301 tacaacaaac ataatcggaa gcgccgattt tagtgaagcc ttttcataaa gagtgtggta
    12361 cacttttgtt gtgtatataa ataaggagga gttcagatga aaaaaattca attaggtact
    12421 tctgatcaga aagcagctgc agtgatctta ggctgtatgc gtatcaacaa tgcggaagat
    12481 ccagtaaaag tgattgagac agcttatgaa aacggcattg atttttttga ccatgcagat
    12541 atttatggaa acggtgaatg tgaagaaatc tttgcagatg ctctagcaaa aacatcaatc
    12601 aaacgagaag atctttttat tcaaacaaaa tgtggaatcg ttccaggtgt gatgtttgat
    12661 ttttctaaaa aacatatcat tgaatctgta gaaaaaagtt tgacccgtct gaaaatggat
    12721 tatgtggatg ctttacttct tcatcgtcca gatacattgg tagaaccaga ggaagtagct
    12781 gaagcgtttg atgaattgga acggtcagga aaagttcgtt attttggcgt cagcaatcaa
    12841 aaaccaatgc aaatcgaatt gctgaaaaaa tatgttcgtc aaccattagt agctaatcag
    12901 ctgcaatttg gcttgaaaca tactggaatg atcgatcaag ggattcatgt caatatgaca
    12961 gatgaagcaa gtatcgatca tgacggtagt gtacttgatt attcccgatt gcatgatatg
    13021 acgatccaag catggtcacc ataccagtat ggattttttg aaggtgtatt tatcgggaac
    13081 gaaaaattcc ctgaattaaa ccaaacatta gaagaactag cagctaaata tcaaacgaca
    13141 ccaactggct tggcttctgc ttggatttta cgccatccag ccaatatgca agtgatcgcc
    13201 ggtacgatga acgtagggcg aatcaaggaa atcgctcaag cttcagaaat taatatgtct
    13261 cgggaagatt ggtacgaatt gtaccgtgct gctggaaaca ttttacctta aagattgtca
    13321 taagcaggtc aaaagggcta taaattaaaa agacagaata acttgaaagc tgttttcagc
    13381 ttcgagatat tctgtctttt tttattgcaa atggaaagag tatcattgta cggatttcgt
    13441 ttattagtta tttttgtttt taaggaaaag ctgtgtccga tattcttttg gagaaatccc
    13501 ttgttttttc ttaaataaat aactgaaata gttagggtca ttataaccaa tttcaaaagc
    13561 aatatctgaa atacgatagt ctgattgctg caataatttt tttgctaatt cgattctttg
    13621 ttctgtcaga taatcgatga atgtttggtt aaacgattga gaaaaaatcg tactgaaatg
    13681 agagggactt aatgcaactt gttgagcgac ccaagttaag gacatatctg gatcggtaaa
    13741 attttctttg ataaaatcca atgcatggtt caaaattggc tgatatttgt tccttgaagg
    13801 ctgtgagggc agagcagtta agaaagaaat gaacatataa actgtcgaac ggtaaagcga
    13861 gtcgtcggcg ctgattgcta agcgttgact ggtggatgtg caatgaacca tttcttgcgg
    13921 gattttcttt tctttcaggc agacatgctg gagtagctta gtcaagacaa ataaacgata
    13981 taatcgtgtt cgagttggtg tgccttgtgg tttcgtcaag tgttcgatgt acttagatac
    14041 ttcgtttttc gataatgaag acaaatcaag tgagaggtca aagtaaaaga cagaatcaac
    14101 tggaagtttc gcttcttgaa tcaaatcctc atagctgata atttgttctt taagggaact
    14161 ggcggtttca agtatcatct gcgtgatctc aaaagatgca ggtaattcgc tgattctttc
    14221 tgcgacagga ccaatggcga cagaaaaatt atttttttgt cccttttcta attcatggac
    14281 caacgtttgg gctacctgat aactggtctc gatcacattt tcttcaagag aatcaactat
    14341 taaaaatttg atgaatcgag aggaaacagc agagaagatc acttgttgat tctccctaaa
    14401 taaaaacttc aaataatcac tgagatgagc gtaatcttca aagtctgtac tgacttggtt
    14461 tgctgctaat aatacggtca tttttttccc tgctaaagag cgattgaaag attcagcaag
    14521 tttcaatgtt tcactgatcg taagtgagcc tttgaacaaa ccgttcaaga aatgattttt
    14581 tttcaattcg aaaacaagat cagaagaagt gtgcgtttct ttttgctgca aatctaaagc
    14641 agttactgct ttttctattg cttgtttcaa ttcgctactt ttaatcggtt taagcagata
    14701 atcatctgcc tgtacctgga ttgctccttt catataatcg aaatcgtcga atccactaat
    14761 aaatatgaga cgagtccaag gaagtagttt ccttgcttct tttgaaaaag tcaacccgtc
    14821 catgaaaggc atttggatat cagtcagaat gatgtcgggc tgtaagtcga taattgaaga
    14881 cagtgccatc tcaccatctc cagcttcgcc gacataagtg attggataat aagaaccaag
    14941 gacagttatt tgttcccgca agttattgcg aatgaggtat tcgtcttcga tgataaaaac
    15001 tttatacatg tttgtttcct ccaattgttt tgggagatag agggatcatc agtgtgacag
    15061 ttgttccttc tcggtatttg ctgtcaatcg tgataggttt ttgatcattt ccgtaataaa
    15121 gaaggagacg cttgtttacg ttgtaaagtc cgtagccgat actgaaatct gaatcgatcc
    15181 ctttggctaa ctctttacgt atttctgcca gtttttctgc agggatgcca atcccgttgt
    15241 ctataacttt gaattggatc atctcatcaa ttttgcatgt ggtaatacga ataaatcctt
    15301 tgtgacggac gtacttcgtt ccatggtata cagcattttc aatcaatggc tgcaagatca
    15361 tttttaaaat agaaacagga aaaagtgttt ctggtatatc gatcttatag tcaagcatat
    15421 ggccgtatct tatttttaag atgactagat aatcattgat atggctaatt tctttttcaa
    15481 gaggaatcca gtcttgtcct ttgcttaaag acgtacgaaa aaagccggaa agtgcatggg
    15541 tcatagtcag agcttcttca aagcagtctt gttcgatcaa agaaataatg gcatccaaac
    15601 tgttataaat aaaatgggga gtaatctgag cttgcaatac acgaacttca ctttgtgcta
    15661 aatggtattg tttcaaagcg ttctcccgta aagactggtt tagatcttcc gccattttat
    15721 tcatgctagc ggtaagtgtt tgtaattcca gtgtttccgg cagggttaaa cgaaagccaa
    15781 ggttccctga agaaagttgt tctgacatct cgatcagctg attgatcggt tcttggacct
    15841 ttctctctaa aaagcgacga ttctttttcg catagaaaaa aatggtacta agaactccaa
    15901 gttccactat tgttaataga atgagcgagc ggacaagtgt cgtatttttt ttgcttgcta
    15961 agtcgatctc cacacgaaca aaatcttgta agatatctga cagcagttga gtgacagaat
    16021 ctacctgatt gatgatctct tcattggtat ccataaaatc ttttgccaag atattattga
    16081 tcagctcgtt ttgataatct tccagtgtat cgatcgtacg tagcgataca tctaacgtac
    16141 taatttcttt aagtgtattt gtattatttt tgatctctcg aatatcattc ctaagttctt
    16201 ctaggatatt ttcctcattg aactcatcta cggttacttg gccagaaatc aagtcccaca
    16261 tactttccaa aactttttga tcaaccttca aagcaattgc atctgctttt tggatatttg
    16321 cgatcgtctg ttgataaatc aaaagctgtt gcgaatataa cagtatgctc agtagcaaag
    16381 ggataacagc aaatagaagc atggctttgt ttgcttgggc aaaaatctgt tttatgctta
    16441 aattttttgt catgatactt ctccagtcaa taatttctgg ttgggatgtt agctatattc
    16501 tgctgggaaa agacagtctc ggaaataaaa atatctttag gtaatgtttt tcctgtttga
    16561 atatttccaa aataacgagt aacggtattg gctacatacc agccgacatc aggtttacac
    16621 tcgactacac aattgacgat tcctgagcgt agcttctcga tcatatcttc ttgtccatca
    16681 atagttacaa tgatcaaatc ttctccaggt ttgattgatg tttcttttat cgcagctaaa
    16741 gcaccgtgag tcatttcatc actatggctg tataatacat cgatttcgga taaatcattt
    16801 gtttctaaat atcgtttcat ctggtctttt cctttcatcc gaataaaatc accgacaatt
    16861 cggtgaacaa tagtcatcgg aggtttatgg tcgatggttc catctttttt tagggttggt
    16921 ttacgattga ttgattccaa aaatccttcg gagcgtagct tggtcggtgt ggagttctct
    16981 gaaccagcaa gttctaggat tctgatttcc tgtttctttg aatttgtgaa gtgattgctg
    17041 acaaataaac ccgcccgatt tccttctgct ttgaaactgg ggccaatgtg ggtgacgaaa
    17101 agctcgggat cattcgtttg aatatgtctg tcgacaagta tgacagggat ccctgctcgc
    17161 tttgcttctt ttaatacaga gtcccacccg gattcttgca aaggagtaaa aatgatcata
    17221 tccactttat aggcaatgaa cgtccgtata tcttggattt gccgttcctg attcatgtag
    17281 ccatttcggt agagaacttg gtaatcttct ttttcaagcg cagtgataat tgattcagta
    17341 tgctttttcc gccagctact ctccgttcct gattgcgaaa aaccaacgac tagtgggtca
    17401 ggtgcttcct ttgtattagg tgtacacccc atcatttggt aagcgattac aacaagggct
    17461 aaaaaaaata aaacacgttt cactttaaat cactccatct ggatttgtta gtactatctt
    17521 atcagataaa agggaaaatc aaagtcaaaa ggaatcaatc gaaaaaaatc caagaaactt
    17581 aaaaaaatcc aagacaaaaa aagtacacct aaaaaattta aagtaaatcc cataaaaaaa
    17641 tcaatgttaa cgctttcttt attagctata ctcggtgtat caattgaaat gggggataaa
    17701 aaatgaaaaa caaatttaga aagattgttg ggactatcgc tattttgacc gcaagcattc
    17761 tattaagtgc gtgtggcaat tcaggatctg cagatgatag tacggggtat gtaggaatcg
    17821 ctatgccgac caaatcagca gaaagatgga tcgctgatgg gaacaacatg gtctcggaat
    17881 tagaaaaact gggttataaa acagatcttc aatatggaga agataaagta gaaaatcaag
    17941 tagcacaaat agaaaatatg attacaaaag gggtagatac acttgtgatt gcttctatcg
    18001 atggttcggc tttaactgat gtgttagcta aagcaaaaga agcagatatc aaggtgatcg
    18061 cctatgatcg tttactgatg aattcagaaa atgtcgatta ttacgcgact tttgataatt
    18121 ttggagtagg tgtatcgcaa gcagcttata ttgaagagca cttaggattg aaagaaggaa
    18181 aaggaccatt cacgattgaa ttatttggtg gctcgcctga cgacaacaac gcactaatca
    18241 attacaacgg agtgatgtct gttttacaac catatatgga caacggtcag ttggtcgtgc
    18301 catctggaca gacgagtttc agtcaaatcg ctacgcttcg ctgggatggt tctacggcgc
    18361 aagcaaggat ggataatctg ttaagcgcaa attatactga ccaaacattg gatgctgttt
    18421 tgtcgccata tgatccaata agtttaggaa tcatttcgtc attaaaaggc gtggggtatg
    18481 ggagtgaaag taaaccttta cctgtgatta ctggacaaga tgcgacagtt gctggcgtta
    18541 aatcgatcat tgccggggaa caaacacaaa cgatctttaa agacacacgt atcttagcaa
    18601 aaaatacgat tgaaatgatc aaagcaatca gtgatggaga agaggttccg gtcaatgata
    18661 ctgaaactta tgataatggg gtgaaaactg tccctacgta tttagcaaat acagtatctg
    18721 tcgataaaga taattatcaa gcagagctga tcgacaccga ttattataaa gaatccgatc
    18781 tgaaaaatta agaggaacca aacaaggaac taaagagatc taagtcagag agaggataag
    18841 aattatggcg gattgtattt taaaaatggc agatatcacc aaaaaatttt ctggaatccg
    18901 agcgttagat tgcgtgaatc tgatgattga acgtggggaa atccatgcat tgtgcggaga
    18961 aaacggagcg ggaaaatcaa cattgatgaa tgtcttgagc ggcttgtatc catatggctc
    19021 ctatgacggg gaaatctttt atgatggaaa gctgtgtcaa tttaaagatt tgaaagatag
    19081 tgaagaaaaa ggcatcgtca tcattcatca agaattagca ctaagtcctt atctttcaat
    19141 aaaagagaat atttttctcg gaaatgaaca agcagaaaga ggcgtgattg attgggaact
    19201 gacagaaaag aaaacatatg aactgctgaa taaagtcggt ttgtctatcg acccgaatct
    19261 tctagtatcc cagattggcg tagggcaaca gcaactagtt gaaatagcta aagcattatc
    19321 aaaatccgtc cgtctgttga ttttggatga gcctaccgct gcattgaatg aagaagaaag
    19381 cgcgaatttg ctgagattga tccgagagtt gaaagagcaa ggtgtaacct ccatcattat
    19441 ttcacataag ctgaatgaaa tcgtggatat cgcagatcga atcaccattt tacgggatgg
    19501 acagacgatt gagacactag aaaatgaaat gatcagcgaa gagcatatta ttcgcggaat
    19561 ggtgggcaga gatttagttg atcgttatcc tgaaagaaaa ccggatattg gagaagttta
    19621 ttttaaagta gaagactgga cggtctatca ccctcttgat cacgagcgaa tcataaacga
    19681 ccacatcaac ttgcatttga aaaaaggaga gattgtagga atagctggac tgatgggagc
    19741 gggcagaact gaattcgcta tgagtctatt tggacattct tatggaagca agatatccgg
    19801 acgtatatta aaggaagggg cagagatatc agttaagacc gttccttcag ccattgcaaa
    19861 cggactcgct tatgtctcgg aggaccgaaa atctttagga ctgaatctgc tgatggatat
    19921 tcgggaaaac gtgacgctga gcagtttgga taaaataagt caaaaaggag tattagataa
    19981 agaaaaagaa gtaactgagg cagaaaattt ccggaaaaag atgcgtataa aaacaaactc
    20041 catccatcaa attgtcagta gtcttagtgg tgggaatcaa caaaaagtcg ttttatccaa
    20101 gtggctgatg acacaacccg atattctttt tttggatgag ccaacccgtg ggatagatgt
    20161 tggggcaaaa tatgagatct acacgattat cgaagaaatg gcagcaaatg gcaaagctgt
    20221 ctgtcttatc tcttctgaac ttcctgaaat attagggatg tgtgatcgaa tctatacgat
    20281 gaacgaagga cgtattacag gagaagtctc gcgagaagat gcagatcagg aagtcttgat
    20341 gaaattaatg acgaaagaag aggtgggtta agttggaaca ggcaaaaaat cttacggata
    20401 aaaaagagaa aaaactaaat ttgaaaagta gagcagctga tctttttggt cgctacagca
    20461 tggtgatcat tttagcggga ctacttattg cgtttcaatt gatgacggat ggtattttct
    20521 ggcgtccatt gaatataact aaccttgttc tgcaaaatag ccatatatta gtactagctg
    20581 cagggatgtt attagttgtg ctcttaggtc atgtggattt gtcggtaggg tcagtgatgg
    20641 cgtttgttgg tgcaattgcg ggcatgatga tggtcaatcg ccaaatgaac ccgtggatga
    20701 cagttatcgc atgtcttgca gtaggcgggc tgatcggcgc atggcaaggt ttttgggtag
    20761 cttatgcagg gataccggcg tttatcgtga ctttagcagg attgctgatg tttcgtggac
    20821 tgacacaagt tgttctagga ggccaatctc tagcaccttt tccagctgtt ttccaaaaaa
    20881 tgtctacggg ttatttacca gacttaagcg aaggaagcag ccttcacttc ttgacattga
    20941 ttttaggaat gatcatcacg atcgtgttag ttgttggtca atggcgaaca agaaatcgtc
    21001 ggaaaaatca tttatttgaa gtggaatctc caacagcttt cttggtaaag gcggtattta
    21061 cagcagtcgt tgtactagga ataacttata tctttgcctc ttatcaaggg tatccgatta
    21121 ttttgatcat tcttggtttg atcgttgcag catatgggtt tttgacaaat aaaacagtag
    21181 caggtcgaca gatttatgcg acgggcggaa atcgaaaagc ggctgaacta tcagggatca
    21241 aaacaaagcg aatcacgttc tgggtattcg tcaacatggg agtcatggca gctttagctg
    21301 gattagtttt agctgttcga ttgaatgcgg caacacctca agcaggtaca tcgatggaac
    21361 tggatgcaat ggcggcagta tattttggtg gtgcttcgac ttccggcggc attggaacaa
    21421 tcatgggtgc gattgtcggt ggcttggtca tgggtgtatt gaacaatggc atgtcgattt
    21481 taggcgtcgg ggtagactgg cagcaggcaa tcaaaggatt gatcttgctt ttagccgttg
    21541 tgctagactt gtataataag aaaaaacgaa caacgtagga gaacgccggt tattcagaaa
    21601 aaagactgtg acaagacatt tgtcacagtc tttttttcgc taaaagcgaa cattcattgt
    21661 ttcgtttgta tagactaaaa ataatatatg tttttttacc attcgtataa attctctttt
    21721 tgctcttgat ttttttgctt aattgagtta aattacatag ggcgctaaaa aaagccttga
    21781 ttaaattaat aatgttcgtg ttttgctgga tgtgaacatc aggaggataa tgtaatgaaa
    21841 gtatttgatt acgaagatat tcaattaatt cctaataaat gtatcgtaaa tagccgttct
    21901 gaatgtgata caacggtcac attaggtaaa catacattca aaatgccagt agtgcctgcc
    21961 aacatgcaga cgattattga tgaaacaatc gcagaattct tagcggaaaa tggttatttc
    22021 tatatcatgc atcggtttga tgaagcagct cgaattccgt ttatcaaaaa aatgaaaaag
    22081 cgtggattga ttacctcgat cagtgttggc gtcaagaaag aggaatattc ctttatagag
    22141 aagttggcag aagaatcgct aaatcctgat tatatcacta tcgacatcgc gcatggacat
    22201 gctaactcag ttattgatat gattcagcat attaaaaaat atttgcctga aacttttgtg
    22261 attgccggta atgttggtac accggaagcc gtccgtgaat tggaaaatgc gggcgcagat
    22321 gcaacaaaag tcgggattgg tccaggaaaa gtatgtatta caaaaatcaa gacaggcttt
    22381 gggactggag gctggcaact ggcagctctt agatggtgtg cgaaagcagc tcgcaagccg
    22441 atcatcgctg acggtgggat cagaacacat ggcgatattg caaaatctgt ccgttttggt
    22501 gcgacaatgg tcatgattgg ttcattgttt gccggtcacg aggaatctcc tggcgaaaca
    22561 aaagtcgaaa atggaatcgt gtataaagaa tactttggtt ctgcttcaga attccaaaaa
    22621 ggcgaaaaac gaaatgttga aggaaagaaa atctggatcc aacacaaagg atctttgaaa
    22681 gatacactag tggaaatgca acaagacctt caatcatcta tttcttatgc tggaggaaga
    22741 gatctggaag ccattcgaaa agttgactat gtcatcgtga aaaattcgat ttttaatgga
    22801 gacacaatct aaaaaataaa taaaatatta ggatgttgtg aaaaagttgt cctgcattaa
    22861 gtaataagaa cagccaaaca aaaatagttc tttatatgtt ttgtttggct ggaattatta
    22921 tcgcagatac aagcttttga acatcattat tcagaataag ggactgtgat aagatttttg
    22981 tcacagtccc ttatgttttg caaaaaaagt ttttcttttt tctcgtaaat tcggtaaaat
    23041 caaaagaaaa aatagacaat agaaaagaga agcttatgtg ggaaaaagta gagaaaaaac
    23101 aaggaccgat ttatcgtcaa attatggaac agattatgag agaaatcgaa aacggaagga
    23161 tcaatcctgg aagtcaactt gaatcagaac gaaaactagg gcaacttttt ggtgtgaatc
    23221 gttccaccgt tgtccatgca ttggaagagc ttagggagct tggggtacta acgagcaaac
    23281 gtggcagcgg atggtttgtt aatcagactg aatgggggaa attcgctgtt ccaagaatcg
    23341 attggcggca aatgatctct cctcgttatg agcagacgga ctggtatgag caaaaaataa
    23401 gagaagcaaa gcagatccaa aaaacatcgt ttcttgattt atacgctagc gagatgccag
    23461 atgagctttt gccgaatttt gagtttcctt cttattctct tgaagaaatc ttagcggaag
    23521 aaaaagaaat ggatctttta ggctatactc cgttacgtaa caaaatcaaa cggtatttgg
    23581 aaagtaaatt tgaattggca ttttccccca accagcttct tgtgactggt ggcggtcagc
    23641 aagcaatctt tttgatttta caaacgattc tttcagctgg agaggggata gctgtagagt
    23701 caccttcttt tttttaccga ttacctcttt tcaaagcttc aggaacgcgt ttatttggga
    23761 taccgatgga tgaagaaggg atcgacttag aaaaactgaa agaatcgatc cataaaaata
    23821 aattgaaagc agtgcttatc aacccgaatt ttcaaaaccc tacaggaaaa gtgatgagtc
    23881 aaaaacgacg agaaaagcta gtagaattgt gtcgacagta tcggcttcca attattgaag
    23941 acgatgtatt tacggatctt tcatttctaa accaagaaga agtccttcct atacgaacga
    24001 ttgatccgga taatgtgtta tacgtcggtt cgttatctag ggttttagga aaaacaacga
    24061 aaatcggttg ggtgatagga cctgagactt ttatctcgca actagctcaa gcgcaagaaa
    24121 tgatggagtt ttcgatgaat atccttacac aaatcacagt ggctaatgta tttaatgaag
    24181 caattgattt gaaaatgcaa gatgtaagaa aaaagttgaa agaaaaaagc cgactgataa
    24241 gcgcttgggg agattcacaa tcgtttttca agcttcattc aattttaggt ggttactatg
    24301 cctggattac ttgggatggg aaaaaactag accccgaact agcagaaaaa gtagtagata
    24361 tgggtcttgg aattgcacca agtctacttt ttgggcaaga tataaatgga atcagaatca
    24421 attacagtag gctggatgaa aaccaaatac cgttgtttta ttcaaagatg aaaattctag
    24481 aaaagtggtt aacagaataa tttcacaaag taaatagatt aaagaatata acagcttgga
    24541 tttaaatttc aagctgtttc ttttttttcg gaaaaagaat acgatataaa gaaactaaga
    24601 aaaaaccatt atttgtacgt ccgttcacga tgtgtaaata aaataaatgt ttgcaacaaa
    24661 actgtataac accaacgttt ttaaatagaa acagatggtt gtaagtttga ttagaaaata
    24721 gaaaaaatgc agatgaaata taaaacgctt tccttattgt gattttattc actaatgctg
    24781 ttaatatgcg gattggcata atttcctttc atcgtgaaaa tttgtgcttt tagcttgtat
    24841 ttctcataaa tccatgataa tttattaatg aagacggtta aatagtgata cagttttatt
    24901 cactgtttca atcgcagaat gaagagattt tcttatagat gagagaggaa tgtgtgacaa
    24961 atggcaaaca aaaaagcacc gatcgacttc caagcactgc tagatgaagt caatgcagat
    25021 ttcccagtat atcaagcact tgatcaagat ggaaacgtag ttaacaaaga tttagtacct
    25081 gatttatccg atgatgaact cgttgaatta atgactcata tggtatggtc tcgtgtatta
    25141 gaccaacgtt caacagcgct gaaccgccaa ggacgcctag gcttttttgc accaactgct
    25201 ggacaagagg caagtcagct ggctagtgct tacgcatttg ataaagaaga tgttcttctt
    25261 ccaggttatc gggatgtacc tcaactagta aaacatggtt tacctttatc tcaagctttt
    25321 ttatggtctc gaggacatgc tgcaggtaat tcttatccgg aagaattgaa agctctacca
    25381 ccacaaatta tcatcggtgc acaatatgta caagcagccg gtgttgcatt aggtttgaag
    25441 aaacgaaaca agaaaaatgt cgtcttcact tatactggtg atggcggttc atcacaaggt
    25501 gatttttacg aagcaatcaa tttcgctggc gcttaccatg caaatgccgt attttatatc
    25561 caaaacaacg ggtatgcgat ctctacacct cgtgaaaaac aaactgcagc aaaaacactt
    25621 gcacaaaaag ctgtcgcagc tgggattcct ggtattcaag tcgatggaat ggatccgctt
    25681 gcagtatata ctgtatctaa aatggcacgg gaatgggcga tcagtggaaa tggaccagtc
    25741 ctaatcgaaa cactgactta tcgttatgga ccacatacac tatctggtga cgatccaacc
    25801 cgctaccgta caaaagacac agatgacgat tggcagaaaa aagatccatt gatccgcttc
    25861 cgtaaatatt tgacggaaaa aggactttgg tctgaagaaa aagaagaaca agtcatcgaa
    25921 gcaactaaag aagaaatcaa agcagctatt gctgaagccg acaaagtacc aaaacaaaag
    25981 gtttcagatt tcttgaaaaa catgttcgaa gtatctccac aatcaatcaa agaacaaatt
    26041 gcaatttatg aagcgaagga gtcgaaataa tcatggcaca aaaaacaatg atccaagcaa
    26101 tcacagatgc cttagcactt gaactagaaa atgacgagaa tgtcgttgtt tttggtgaag
    26161 atgttgggaa aaacggagga gttttccggg caactgaagg attgcaggaa aagttcggag
    26221 aagaccgagt attcgatact cctttagctg aatctggaat cgcaggattg agtttcggtc
    26281 ttgcgttaga aggatttcgt ccagttcctg aaatccaatt ctttggtttt attttcgaag
    26341 caatggacga agtagtagct caaatggctc gtacaagata tcgaatgagc ggcactagaa
    26401 atctacccat cacaattcgt tcgccatttg gtggcggtgt tcatacacca gaattgcatt
    26461 cggataattt agaaggtttg atcgctcagt ctcctggtat tcgagtagtg attccttcca
    26521 acccttatga tgcaaaagga ctattgattt cttctattag aagtaacgat ccggttgttt
    26581 tcctagaaca catgaaacta tatcgctcat tccgagaaga agtgccggat gaagcttacg
    26641 aagttccttt ggataaagca gcagttactc gtgagggaac agatgtatct attatcacat
    26701 acggagcaat ggtgcgtgaa gcaatcaaag cagcagacaa tcttgcaaaa gaaaatatat
    26761 ctgttgaaat cattgatctt cgtacagttg cgccattaga tgtagaaacg atcattcaat
    26821 ccgtggaaaa aactggtcgc gtagttgttg tccaagaagc acaacgacaa gcgggcgttg
    26881 ccgcgcaagt agtttctgag atctcagaac gcgctatcct ttcattagaa gccccaatcg
    26941 gacgagtttc tgcacctgat acagtcttcc catttggaca agcagaaaat gtctggttgc
    27001 caaatgcaaa agatatcgaa gacaaagtaa aagaaatcgc ggaattttaa gaaaagaagg
    27061 gacgaaacaa agtcatggct tatcaattta aattgccgga tatcggtgaa ggaatcgcag
    27121 aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat
    27181 tactagaaat tcaaaatgac aaatcagtag aagaaatccc gtcacctgtt acaggaacgg
    27241 ttaaaaatgt gattgttcca gaaggtaccg tagcaaatgt tggggatgta ttagtagaaa
    27301 tcgatgcacc aggacacgaa gacaatgaag gggacagtgg tgtagccgca gaatcccaaa
    27361 ctccagctaa accggctgct gaacccactg ttgatacaga atcagctgga tcgtcaagcg
    27421 aaggagtctt ccaatttaag ttaccggata tcggtgaagg aatcgcagaa ggtgaaatcg
    27481 tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta ctagaagttc
    27541 aaaatgacaa atcagtagaa gaaattccgt cacctgtcac agggacagtt aaaaacgtga
    27601 ttgttccaga aggcactgta gcaaatgttg gggatgtatt agtagaaatc gatgctcctg
    27661 gacacaatag tgcaccgtca actagtgcgc catcagctga agcaccaaaa gaaaaagtgg
    27721 aaacatcagg ttcagcaagt gtcgttgaag ctgccgatcc aaataaacgt gtattggcta
    27781 tgccatctgt tcgtcaattt gcccgtgaaa aagatgtaga tatctcacaa gtaactgcaa
    27841 ctggaaaagg tggcagagta acaaaagagg acatcgaaaa cttcttggca ggtggtccaa
    27901 gcagtgcacc agcgaaatca gaagcgccag aagcggcagc accgaaagaa gcagctcctg
    27961 cagcagaaag caaaccagct gcaccagcaa aaccattcaa gtccaactta ggagacttgg
    28021 aagaacgtgt agcaatgacg ccaacacgta aagctatcgc taaagcaatg gtcaacagta
    28081 agcatacagc tccacacgtg acattgcatg atgaagtaga agtgtctaaa ctatgggata
    28141 atcggaaacg atttaaagaa gttgctgctg caaatggtac gaaacttact ttcttgccat
    28201 atgtcgtgaa agctttaaca gcgactgtta agaaataccc agtattgaat gcatctatcg
    28261 acgatgcaaa ccaagaaatc gtttataaac attactacaa tatcggtatt gcaactgata
    28321 cggatcacgg actatatgta ccaaatgtga aagatgcaga ccgcaaagga atgtttgcta
    28381 ttgcagacga aatcaatgag aaagcaaaat tagctcatga cggcaaacta tctgcagaag
    28441 atatgcgcaa tggaacaatt acgatcagca atatcggatc tgtaggtggc ggttggttta
    28501 caccagtcat caactatcca gaagtagcta ttttaggtgt aggaacgatt gcacaacaac
    28561 caatcgtaaa tgctgaaggc gaaatcgttg tcggtcgtgt gatgaaactg tcattaagtt
    28621 tcgatcaccg tatcgtagat ggagcaactg cgcaacaagc aatgaataat atcaaacgtc
    28681 ttttagctga tccagagcta ttaatgatgg aaggatgata aacaaatggt agttggagat
    28741 tttgctgtag aattagatac agttgtaatt ggtgcgggac ctggtggcta tgtagctgcc
    28801 atccgcgctg ctgaaatggg acaaaaagta gcaatcattg aaagagagta tattggcggt
    28861 gtttgtttga atgtcggctg tattccttca aaagcactga tagctgcagg acaccattat
    28921 caagagtcac tagattctga aatgtttgga gtaactagtg aaaacgtcaa actagatttt
    28981 gcaaaaacac aagaatggaa agaaaacaaa gttgtccata cgttgacttc tggtgttggt
    29041 ttcctattaa aaaaacataa ggttgaaaca atcgaaggag aagcgttctt cgtagatgat
    29101 cacacactac gcgtcattca tccagattca gctcaaactt attcgttcaa taacgcaatc
    29161 atcgcaacag gttcccgtcc aatcgagatt ccaggattta aattcggagg acgtgtgtta
    29221 gattctactg gcggattagc attgaaagaa gtacctaaga aatttgtgat catcggtggc
    29281 ggtgtgattg gtgccgaatt gggcggagcg tacgcaaact taggagcaga agtaacgatt
    29341 cttgaaggct caccacaaat tttaccaact tacgaaaaag atctagtaaa attagtagaa
    29401 gatgatttca agaaaaaagg cgtaacagtc gtaacgaatg caatggcgaa agaagcagtc
    29461 gataacggtg acagcgtgac agtgaaatat gcagtggacg ggaaagaaga atccgttact
    29521 gctgattatg tgatggtcac tgtaggtcgt cgtccgaata cagatgatat gggcttagaa
    29581 caagcgggtg tcgaagtagg agaacgagga ttgatcacgg tggataaaca aggacgtacg
    29641 aacgtaccta acatctatgc catcggtgat atcgttccag gggcggcatt agcacacaaa
    29701 gccagttatg aagcaaaaat cgctgctgaa gcaatctctg gcaaaaaagt agctgtagat
    29761 tataaagcaa tgccggcagt agccttcact gatcctgaat tggcttctgt aggtatgacg
    29821 atcaaagaag caaaagatgc tggtatcgaa gcaaaagcat ataaattccc attttcaggg
    29881 aacggtcggg cattatcgct tggtaaaaca gaaggattca tccgtctagt tacgacgatc
    29941 gaagataacg tgcttattgg cgcgcaaatc ggtggagtcg gcgcaagtga tatggtctct
    30001 gaattagctt tagcaatcga atcaggaatg aatgctgaag atatcgcctt gacgattcat
    30061 cctcatccat ctcttggcga aatcgtcatg gatgcttcgg aattagcatt aggtttacca
    30121 atccatattt gatttgaata atacaggcga aacgcaactg aaaaatcagt tgcgttttgt
    30181 tttttagaat tatcaataat attattatgt aaacttaaaa atatgacaac ttcttggttt
    30241 attaccactt cctatgctac actgaagagg aatatgaaat aggagagttg aagatgcaag
    30301 aatatcagta tccacttgat ttagattgga caacagacga aatggtcatt gtaatgaaga
    30361 tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa caatgaagct tttttgaaga
    30421 cttatcagca gtttaaaact gttgtcaaat ccattggtga ggaaaaacga ttgggacggg
    30481 aatttgaaaa agcttccggt tattctttat atcgtacagt gcagcaagca aaaaaaaatc
    30541 caaacaaaac tttgagaatg aacgggtgag ataaatggaa cagatgattg atcatatcaa
    30601 gtcctggctt gaagaagctg gtgagaaaat caaatcttct caacaaacat taacaatctc
    30661 gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat cgaaacatcc agaattttct
    30721 aatcgaaaaa atccattcct ttgatccaga ggcgaaaata ttggcagaag aagaagggtg
    30781 taatcaatta tcggatctcg atggaagagt ctttatcatc gatcctattg atggcacctt
    30841 gaattttgtg attgaagggg aaaatttttg tattatgctc gctgtttatg aatccggcgt
    30901 tggaaagctt ggtttcattt atgacgtgat gagaaatgaa cttttttggg gtggaaaagg
    30961 aataggcgtg tataaaaatg atgagccatt gcctagacca gcaaataaat ccttatcgga
    31021 aggcttactt ggtgtgaata gttatctttt cggtcataat aaatatggaa tccgagaaat
    31081 tgcggaaagc agcatgggga cacgtatgta cggatgcgct ggtcttgaac tagtggcaat
    31141 cttgaaagga aaccacattg cttatatttc aaaccttagc ccttgggatt atgcagcggg
    31201 aaatgtctta ttggaagagt ttggtatggt aacaagcggt ctttttggta ctcctttgac
    31261 atttgaaaaa agggaatact atttagcagg gacttcttgt gcatatgcag aaatcgcaga
    31321 ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat tcacaaaaat gataaaaacg
    31381 agcaaaagat taattttctc gaaaaaatga aaacatttta ctatatttca tgaaaaaagt
    31441 ctgctataat tagcagtaga gtaaaatttg ccctgtcata aagaaccaat aaaaaaggag
    31501 ttagttacat tgaattatag aaacgatatc cgtaacgtcg caattattgc ccacgttgac
    31561 cacggaaaaa caacattagt tgatgaacta ttaaaacaat cagatacatt agacgcacat
    31621 acgcaattgc aagaacgtgc gatggactca aatgcgctag aaaaagaacg tggtatcacg
    31681 attttagcca aaaacacagc tgtagactat aaaggtattc gcgtaaacat catggatact
    31741 ccaggacacg cggacttcgg tggcgaagta gaacggatca tgaaaatggt tgacggcgtt
    31801 gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa cacgttttgt attgaaaaaa
    31861 gcactggaac aacatatcac accaatcgtt gtcgtaaaca agattgacaa accttctgct
    31921 cgtcctgagc atgtcgtaga tgaagttctt gaactattca tcgaattagg agcagatgat
    31981 gatcagttag actttcctgt gatttatgca tctgcactaa atggtacgtc aagcttgtca
    32041 gatgatccag cagatcaaga accaaccatg gcacctattt ttgatacaat tattgaaaaa
    32101 atccctgctc ctgtcgataa cagcgacgaa ccattacaat tccaagtttc attacttgac
    32161 tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat ttagaggaac aatcaaagta
    32221 ggagaccaag tagcattaat taaattagac ggaacagtta agaaattccg tgtaacgaaa
    32281 ttatttggtt tctttggact aaaacgtcta gagattcaag aagcaaaagc tggagacttg
    32341 attgctgtct caggtatgga agatatcttt gtcggtgaaa cagttactcc tgtggatcat
    32401 caagatgcgt tacctatcct tcacattgac gaaccaacat tgcaaatgac tttcttagtt
    32461 aataattcac catttgccgg tcgagaaggt aaattcgtaa ccgctcgtaa gattgaagaa
    32521 cgtttgatgg cagaacttca aacagatgta tccttacgag ttgaaccaac aaactctcca
    32581 gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt ctatcttgat tgaaaacatg
    32641 cgccgtgagg gatacgaatt gcaagtatct cgtccagaag ttattgaaaa agaaatcgac
    32701 ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata caccagaaga atatatgggt
    32761 agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc aagatatggt ccatacagga
    32821 aacggacaaa tccgtttgac attcttaaca cctgcccgcg gtttgattgg ttattcaact
    32881 gaattccttt caatgacacg tggttacgga atcatgaacc atacttttga ccaatatctg
    32941 ccaatgcttc ctggtcaaat tggcggacgt caccaagggg cgcttgtttc gatcgataca
    33001 ggaaaagcaa cgacttattc tatcatgagt atcgaagaac gcggtacagt cttcgtagaa
    33061 ccaggtacag aagtatatga aggaatgatt atcggggaaa acagccgtga taatgatttg
    33121 actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac ggtctgctac taaagaccaa
    33181 acatctgtca tcaaaaaacc aaaacaattg acgttggaag aatctttgga attcttgaat
    33241 gatgatgaat attgcgaagt aacaccagaa agcattcgct tgagaaaaca aattttagag
    33301 aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat ctgtaaaagg atgaagagag
    33361 aaaccagctt gttataaaaa atagctggtt tctttttttg tccaactctt ttctgtggag
    33421 aagtcatctt tgtcaatgga cataaattca gaaaatacgc cgaaagacgt ttaaaagact
    33481 tttaaaaaac ttatttctag agagtttttg tttcataata agataatgtt tcacgaaagg
    33541 agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc aagagaaagg agcgggaaga
    33601 tgaacgactt attaaaacaa tactttggtt atgatgagtt tagacctggt caaaaagaaa
    33661 ttatccagaa agtcattgat caggaaaatg tattaggaat catgcctacc ggaagtggga
    33721 aatcaatctg ttaccagctt ccagctttac tattagatgg gcttactgtg gttgtgtccc
    33781 ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa tcaattaggt atacccgcca
    33841 cttttatcaa tagctcgtta gatggttacg aaacagctag acgcttccaa gaaatcgata
    33901 gacagcagta ccgtttactt tatatcgctc ctgaacgttt tatcatgccg gattttatcc
    33961 aagcaatgaa gcgatggaac gtccgtatga tcgctattga cgaggcacat tgtatctctc
    34021 agtgggggca tgatttccgc ccaagttatt tacagatggc gaaccagcta gatcaactgc
    34081 caaatcgtcc tgtaattgtt gctttgacag ctacagcgac tgtgcaagta gctgctgaca
    34141 taaaaagact tttaaaaatt ccagaaaaca accatattca gactggtttt gaacgagaaa
    34201 atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca atatctaatc gaatatttaa
    34261 agatcaacaa aaatcaatct ggtatcattt atgcagcaac gagaaaagaa gtagaccgtc
    34321 tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag atatcatggt ggtctaaatg
    34381 agaatgagcg aacagaaatg caagaagcct ttttatacga tcgcttgcag ctgattgtag
    34441 cgacaaatgc atttggtatg ggtataaata aaagcaatgt ccgttttgtt atccattatc
    34501 aaattcctgg ctctctagaa gcatactatc aggaagctgg tcgggcggga agagatggtc
    34561 tttccagtga agccatcctg cttttctctc ctcaagatat ccaagtccaa aagtttttta
    34621 tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga atatgaaaaa ttaaaagcga
    34681 tgacagaata cgtgtatatt gagtcctgtc ttcagcaata catcctaaac tattttggcg
    34741 agacaagcag tccgtgcaac cgttgtggta actgtttaga tgacagagaa atagtagaag
    34801 taacgacaga agcacagatg gttctttctt gcttaaaacg tatgggtgag aactatggta
    34861 aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca aaagctgcaa gctttaggtt
    34921 ttggacattt atctacctat ggactgatga aaaaccaatc gcaaaaagaa acgatgcagt
    34981 taattgaata tttgatatcg aatggctatc ttctgaccat caatggtgag tatcctgttt
    35041 tgaaagtaac agaaagaggg atacaagtat tgaaaggaca agaatctgtt tatcgaaagg
    35101 aacccaagaa agtccagcag ctatcggatg aagaaaccga tacactattt gaagtgctgc
    35161 gggaactgcg aacggatcta gcatcagaag caggcgttcc cccgtatgtc gtattttcag
    35221 attccacatt gaaagaaatg agccgaatac gtccatcaag cagattggag atgcttcaga
    35281 taaaaggagt tggccaaagc aagcttgata aatatggcga agccttttta tctcggataa
    35341 aaaatgcgga ctctaacgtt ctggataagt agaaagaacg aatacgtaaa aatgaataaa
    35401 aacaactggt taaatctgtt ttttttgctc cttatgaaaa tcaaatatgt tataattaag
    35461 gaattagaat gggaggtaag cacagatgga ttctttgtcg aaaaaacttg cgatccaagt
    35521 attaaatgat gaagcagaac ggatcaaacg attaatccga aatcaaaaca acagtctctg
    35581 tatctcacaa tgcaaagcct tcgaagaagt ggtagataca cagatgtacg gcttttcccg
    35641 gcaagtcggt tttgctgtcc gtataggagt aatcggaaag aacgagggcc agctgatgtt
    35701 aagtgagcta gaacgagaat tgaatcagtt atatagtgag gtttaccaag aaatttatga
    35761 taaaaaagaa gttggcaagg aggaatagac ttgcctaaga aagtgaaaaa aaggcatcta
    35821 ttagactata gcatcttgat tccctattta attttatgtg tattgggatt gatcatggta
    35881 tatagttcta cgtcgtatct attattagaa aacggacaaa atccgtccgc atcagtcatt
    35941 aaccagagta ttttttgggt gcttagcttg attgctatcg cactacttta taaaatgaaa
    36001 acagatgttt tgaaaaacca gcgtttgatc atggcagcca tcgcagtttt gacgattttg
    36061 ctgctgattg ttgtgttttt tggtaaggag atcaatgggg cgaaaggttg gctgcaaatt
    36121 gcaggattct cgatccaacc tgccgaatac ctaaaaatca tttctatctg gtacctgtct
    36181 ctgactttat ctaaaagaca aaacagtgta caaaaagatt ttcttgggac tgtgaaacgt
    36241 ccacttgcaa tggttatcgg acttactgca ttagtcgcta ttcttcccga ttttggtaat
    36301 gcggcagtta tctttttgat tattttggtt ctgcttttag ccagcggagt caattatgtg
    36361 tatactttga ttgtaggagt aggaggcttt tgtctaagta cttttacgat ctggctgatc
    36421 aatatcacaa acggaaagat cttacccgga cgtttacagt atatctataa tcgatttgct
    36481 atttaccaaa atcctttctc agacgaatta aataaagggc atcaactagt caacggctac
    36541 tatgccatgt tcaacggagg actatttggc aggggattag gtaacagtat ccagaaaaaa
    36601 ggatttttac aagaagcaca aaccgatttt atttatgcaa tcgtagtgga agaactggga
    36661 gtgattatgg ggattttgat attagcgttg ctattcttta tgattgtgcg cattgtcctt
    36721 gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt gcattgggat cggcgcaatg
    36781 tttttgatcc aagtatttgt caatcttgga ggaattactg gcgttattcc tttgacaggg
    36841 atcacgttcc catttttaag tcaaggaggc tccagtttgc tgatgctttc gatttgtgtc
    36901 ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga gcttaggttt ataataaaaa
    36961 aagatgtagg agtggttcga tgaaaaaagt tttagttgct aacaggggtg aaatcgctgt
    37021 acgtgtattt cgtgcttgta cagaattagg aattaaaact gttggaatct atgcagaaga
    37081 agatgaatat tccgtacacc gtttcaaagc ggatgaagca tatttagtag gacaaggaaa
    37141 aaaaccgatt gatgcgtatc tagatattga agggatcatt tctatcgcga aagaatgcgg
    37201 agctgacgct attcatccgg gttacggtct tttgtcggaa aacttgaatt ttgcgcaacg
    37261 gtgcgaagaa gaaggcatca tttttgttgg acccaaactg catcatttag atatttttgg
    37321 agataaaatc aaagcaaaag ctgcagctat cgaagcgggg attgcttcga ttccaggcac
    37381 agatggacca atcgcttcta tagacgatgc cttggagttt gcgaaacaat acggttatcc
    37441 gattatgatc aaggcagctc taggcggcgg cggacgcggc atgcgtgtag ctcacgatga
    37501 aaagagtgca agagagggct acgaacgtgc gaaaagcgaa gcaaaagcag cctttggtag
    37561 tgatgaagtg tatgttgaaa aatacatcgc taatcctaag catatcgaag tacagattct
    37621 cggggatacg catggaaacg tcatccatct ttttgaaaga gattgttctg ttcagcgccg
    37681 tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg aatgaacagc aacgtcaaaa
    37741 aatctgccag gcagctgtcc aattgatgaa atatgtcggg tatgtcaacg caggtacagt
    37801 agaatttctt gttgaaggag atgattttta ctttatcgaa gtcaaccctc gcgtccaagt
    37861 ggaacatacg atcacagaaa tgattacaga tattgatatc gtaacaacgc aactgttgat
    37921 tgctcaaggt ttggatctgc ataaagaaat cggattgcct cagcaagaag gaattaaatt
    37981 aaatggttca gccattcaat gccgaatcac tacagaagac ccgttgaata actttttacc
    38041 agacacaggg aaaatcgata cgtatcgttc acctggtggt tttggcgtac gtctggatgt
    38101 cggaaatgct tacgcgggct atgtagtgac accgtatttt gattcattgc tagtaaaagt
    38161 atgtacgcat ggtgctacct ttgaaacagc cattcagaaa atggaacgtt gcttgaaaga
    38221 atttagaatc cgtggagtga aaacaaacat tccatttatg ctcaatgtca tcacacatcc
    38281 tgaattccaa tcaggaaatg caaaaacaac cttcattgat agtacagcaa cattatttga
    38341 atttcctcga ctacgagacc gcggaaacaa aacgatgaaa tatatcggtg aaattactgt
    38401 gaatggtttt ccaggtatcg aaagtggcga aaaaccattt tatgaagaac cacgtatgcc
    38461 taaagatcta atcacaagaa ctgattacgt aacggctaag aatgtattag atgcgaaggg
    38521 tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat ttgttgttga cggatacgac
    38581 atttagagac gcccatcaaa gtctgctagc aactcgtgtt cggacaaagg atttcaagca
    38641 gattgcacgt ttgactggag aaggtcttcc tgaactcttt tcaagtgaga tgtggggggg
    38701 agcaacattt gatgtggctt accgcttttt gaatgaagat ccttggcaac ggttgagaaa
    38761 aattcgttct ttaatgccta atacgttgct gcaaatgctt ttcagaggtt caaatgccgt
    38821 tgggtattct aattatcctg acaatgtatt ggtggaattt gtcaaagaag cagcagcgca
    38881 aggaatcgat gtatttcgaa tctttgatag tttgaactgg actccacaaa tggaaaaaag
    38941 tatccaagct gtacgggata ctggtaaaat cgcagaggca gctatttgct atactggcga
    39001 tatcaacgat ccaagtcggg caaaatacaa tgttcagtat tataaagaca tggcaaagga
    39061 actagaacaa ctgggtgcgc atatcattgc aatcaaagat atggctgggc tgcttaaacc
    39121 gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg acagatctgc caatccatct
    39181 gcatactcat gataccagcg ggaatggaat catcacttat tctgctgctt caaaagcagg
    39241 agtagacatc gttgacgtgg caatgagtgc aatgagtggg aatactagtc agcctagcat
    39301 gagcagcttg tattatgcac tggtcaatgg tccacggctg ccagaaatca cgattgaaaa
    39361 tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg tattacaaac cgtttgaaaa
    39421 cggcttgaat gctcctgaaa cagaagtata tatgcatgaa atgccaggag gacagtattc
    39481 gaatctacag caacaggcaa aagctgtcgg attaggtcat cgctgggatg aaatcaaaca
    39541 aatgtatcat actgtcaact tgatgtttgg cgatatcgtt aaagtaactc catcatctaa
    39601 agtagttgga gatatggcac tatttatggt ccaaaatgat cttacagaag aagatattta
    39661 tgaaaaaggc gaaacactaa gtttccctga atctgttgtg actttcttcc aaggagaact
    39721 agggcaaccc gttggcggat ttcctaaaaa attgcaaaag attattctta aaggcagacc
    39781 tgcattgaat gaacgtccag gtcttttggc tgaatcagtc gacttcaacg aagtaaaaaa
    39841 agaattggca gaaaaaattg gctatgaacc aaaacaagac gaagtattaa gctatttgat
    39901 gtatccgcaa gtctttttgg actatcaaaa agcatataat caatttggcg atgttacatt
    39961 actggacaca ccaacatttt tccaaggtat acgtttgggt gaaacaatca atgtacaaat
    40021 cgaacgcgga aaaacgctta tcattcgttt agatgaaatc ggagaacccg atatcgaagg
    40081 gaatcgcgta ctgttcttca atttgaatgg acaaagacgt gaaatcacta tcaaagataa
    40141 ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa ccgacaaacc gagaacaaat
    40201 tggagcaacg atgtctggtt ctgttttgga tgtattagtg aaaaaaggcg ataacgttaa
    40261 aaaaggtgat acgttgatga ttactgaagc gatgaaaatg gaaacggcca tcgaagcacg
    40321 ttttgacgga gaagtcgcac atgtatatgt gtcttcagga gacactattt cgtctggtga
    40381 ccttttgatt gaagtgactg agaaataata agcgaaaggt ggggaaacat gaaaagactg
    40441 ggtttgtttt ggctgattct gctattagtt attgtggcat tctacatgga accagtcttg
    40501 tttccgccaa aacttgaagt gaaggaaaac tctgatcaac aaatatcaaa ccaaatgaca
    40561 acgtggaaat accaggagct taaagcaact ggtttttcta cttatatcgg aaaaccgatc
    40621 gatcagctgg aaaaggattt tggccaagcc tatgatcgat taactagcgg atttggtttt
    40681 gaaacaagat attatgaaaa taaagctagt catctcacat ttgaagcgaa catccaagac
    40741 agcagagtca caactgttaa agtactgcaa acagggcctg ctgatatcgc gccgttttat
    40801 ttagggatga cgatgcaaga tcttacgaaa atcactacta tctatactaa tttcactttt
    40861 gagtaccatg atacagaagt tggaatcgaa ctaatggaag aggatatgaa ttatcgtccg
    40921 ctaattgctt ttgataacga cacttttgct attttatttt ttgatcaaac aacaaacggc
    40981 ctattttcag ttgtgtattt agataaagat agtcttttga aactattgcc atatcaagtg
    41041 tttggtgaag gccttccaca ttatcaagca gataaatcgg ctgattggga atcaatcaat
    41101 cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc ttagaagaga agacgaactt
    41161 cccatataca atcaaacact tgcctttcaa aaccacagtc agctgctact acatgatttt
    41221 ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg ctgattggca aaaatcgcta
    41281 ggcagtgctc aaacgacagt gaaattctca ctgacgaatg atgaattgga gaagttaatc
    41341 agtaaacaaa aattagaaaa tactagtggg ctatttattc atccggcgat tgacgctact
    41401 ttttcgtttt tatattggta cagcgatcct tacaaccatg accgtttcat ggacccggga
    41461 acggattctg taggcattgc tttttctaaa gaaaatatgt tagtcttatt gcaagaagaa
    41521 aacggagaaa gtagtgatag cagtgatcgt taacgaagag ctgtttcaac tggaggatca
    41581 atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg atggcttctt atcttgaaca
    41641 taaacagtca atggaagaat ctgcaaaagt ttgccagttg aaagaagcgt tcttgtctaa
    41701 gaaagaagca tttgaaaatg tagcggcata tggtgagtat gcacctgatt atcgtgaaaa
    41761 aaaacgagct gttcgaaaag caaaaagaca gcttgatatg gatgagcatg ttgcagcttt
    41821 ccgatatagt gaaacacagc tgcaaagtct gttggatcaa attgtccaag tacttgccga
    41881 agaagtttct aatgaaatca aagtagatgc aggtagcccg ttttttgaaa cgaaaaaaca
    41941 ctttggatgc ggaggtaatt gtcatgcaat tagacaatag tgaatttgaa ataacaaaac
    42001 gacgcgggct gatcgtatgg gtctacagct taaaacaatt aaagaatctg aaaaagttcg
    42061 gcttgttgca ttatgtctca agaagaatga aatatgtcgt gctttactta aatgaagaga
    42121 cattggaaga aacagaagaa cgaatccgta aattacattt tgttcgaaac gtagaacgtt
    42181 cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat aggaaacaaa acagtattaa
    42241 aagacgaagg tttcgaaatt gaagagctaa atacagaaat ccgattagct gaacatcttt
    42301 aaaaatctct tatgagcagt ttttaaataa aaaataactt ggataacgat cgatttttga
    42361 tcattgtcca agtatttttt tggtatacta gtaccagtac catttctaag gagtgagtag
    42421 atgcggataa tatcaggaaa ttatggcggg cgccgtttaa aaagtttagc aggggcgaac
    42481 accagaccaa ctatagataa agtaaaagaa tccatattca atatgattgg accatatttt
    42541 gatggagaga cagtattgga tctgtttgca ggaagcggtg gtttagctat cgaagcagta
    42601 tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt accaagcgct aaaaatcatc
    42661 aaagaaaaca tcgaaattac gaaagaacca gagaaattta ccacaataaa acttgatgcg
    42721 gacaaagcaa tcgaacggct agcagcggag aaacaacaat tcgactactt gtttcttgat
    42781 ccgccatatg caaaacaaaa aataatcgat caaatccata aaatggaaga actgggactt
    42841 ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa cagtcgaatt gccagaaact
    42901 gtggctggat tccatcaaat aagaaaacag atttatggca taacggccgt aactatctat
    42961 agaaaagagg gaaatcaatg aaacgtgcat tattcccagg aagttttgat ccttttacaa
    43021 aaggtcactt agatacggtg gaacgtgcgg ctaaattatt cgatgaggta gtaattggtg
    43081 tttttatcaa tacttcaaag aaaagtttgt ttcccccaga agaaagaatg acattgatca
    43141 caaaagctgt ttctcatctt cctaatgtaa aagtcatgca tcaagaaaat caattgacgg
    43201 tagaaacagc aaaagagatt ggtgcagatg cgttgatcag aggcatacga agtatcaaag
    43261 actttgaata tgaacgagag attgctcaga tgaatcatca tctatatgat gaattggaaa
    43321 ctgtcttctt attagcaaaa ccagaatata gtcacgtgag ttccagtatt ttaaaagaag
    43381 tactgcactt tggcggagat gtgagcagct acttaccgcc agtgatcaac gaagctttag
    43441 ctggaaagcg tgaatcaaat gaaagctaaa caatggttga aaacagtact gattatcata
    43501 tttggaatgt tgatcattgg atttgtagta gttcccttgc cgtattatgt agaatcaccg
    43561 ggagccacga tcgatctgaa agaactaatt acggtcaatg ataaggaaga taaaggttct
    43621 gggtctttct ctcttacctc tgttggtatc aggcaagcta caggtttctt agctttaaaa
    43681 gctaaaatga ccccttttga agaattgatc agtgaagaag aattgactgg tggggcgaca
    43741 aatgaagaat ataatcaaat gcaacagtat tatatggatt cttcccaaaa tgcagcaatc
    43801 gaacaagcgt taaaacttgc agacaagcct tataaaatgg aattcaaagg cgtatatgtc
    43861 atgagtgtag agaaaacatc gaatttcttt ggcaagcttt cagttggaga tacagtaaca
    43921 aaagtagacg ggaaaagctt tcaaagtaca gaagcattta tggattatgt aaaaagccaa
    43981 aaagttggac aaacaatcga aatcagttat ttgcataatg gtgaagaaaa aacagcttct
    44041 ggtgatctga tcgagttacc aacagataaa aaggcaggaa taggtatcac gcttactgac
    44101 cataccgaga tcgaatcaga tacagatgtt cgtattaatt ctggctctat cggtgggcca
    44161 tcagcgggat tgatgttcac tttagaaatc tatgaacaag tcactggtaa aaacttgcga
    44221 catggtaagc aaattgcagg aactggaacg atcaacagtg aaggagaagt tggccgaatc
    44281 ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg gcgtagagat tttctttgca
    44341 ccagatgata cgatttcaga cgatgttaag aaagaatatc ctgaaataaa aaccaactac
    44401 gaagaagcaa aagctgccgc taaaaaattg aattcctcaa tgaaaatcgt accagtaaaa
    44461 acagtacaag aagcgcttga ctatctagag aaaatgaaat gaaactaaat aattaaagag
    44521 gggttgtgac acgagttctg tcacaacccc tctttttcgt caaaaatctt cacgataaaa
    44581 ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa gggacgattt tgccagagct
    44641 cactgtttct tcaattttaa atgaaaggag taatgaatga tttttactgg tcattctttg
    44701 aaaaaagaac aatgatagaa aaaatcagca agaaaatact agcttcaccc aaattgatca
    44761 tttggatgtc tatgatagtt cttatctttt gtatcgcaag tggtttttta ttatcaaatt
    44821 tgctatttta tagagaaaca gaagagactg ctgcaaaaga aatactttct tcgtttagtt
    44881 ccagtggaga agatccatca gctgaaggaa acgatgtaga agaggatgct cgtccaaagg
    44941 tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg tatctattcc ttttcttcag
    45001 aagaacgggt ctatgatgta ctaaagcgtg caggtggatt attagaagaa gcggatagcg
    45061 atcgaatcaa cttttctgcg aagatagaag atcagcaagt cctatatatc cctgctgtag
    45121 gtgaagagct gccagaacat cttgatcagt tggcatcctc ggaaggaaaa cagcctacag
    45181 cagattccga gcctcccaaa ataaacatca atacagccac tccatcagaa cttcagcaga
    45241 ttcctgggat cggatcagtc aaagcgcaag aaatcattcg atttagagaa gaaaacggat
    45301 ccttccagaa agtggaagac ctgcaggaga tttctggtat cggtgaaaag acagttgaaa
    45361 aactaaaaaa cttcgttaca attaaataga atgattcaaa atcgactggc acgagcaata
    45421 aatggacgct aatggatact aagaataatc caattctaag agcttggcag gacgaggagg
    45481 tagtattttt ggataggaaa tagcttgatt gttcgcaaag aaacattaaa aaaaccaatg
    45541 ctgaatgtct atctccaaaa taaaatatct gggattcata ttatgaatac ggctgtcagc
    45601 ggcaataaca gtcaagctct acgagaacgc tttgccaaag atgtacttag ctatacagca
    45661 gataaagtat ttattctcat tgggacgaat gatctagcag agcataaaca actttctaaa
    45721 gaaacgtacc aaaaaatctg cagtggatga tagttcagct gaaaaaagac tatagttctg
    45781 atcatgcttt ctttatcacg ccaccagcag tcgatgagac aaaacaaaaa tatcggaata
    45841 acaagttgat caaagaatat atctctgttc tcacagaagt agcaaaacgc catgattgtg
    45901 aagtatcgat ttttatacgg aatttcttga acaaatggat tcgaagaatt tttataatgg
    45961 gctaatggat gacggacttc atttcggcag tgaagggtag gatcttttgt cagacaggat
    46021 gattcgttat atttaaagaa tattttgttt aagcaatgag tactttttaa tcaacttgaa
    46081 aaaacggagg aatataaaac atgttagaaa aatcttcaaa agcatgcgat acgaataaag
    46141 aaaaagttac tgataagtca cataatgaac gcattccttg ggaccagtac tttatggctc
    46201 aggcagtact gttatcttta cgtagtacgt gtacacgttt agaagtagga gcgacactcg
    46261 ttcgagaaaa aaggatcatt gctggcggct ataatggagc agtcagtgga gatgtccact
    46321 gcatagatga agggtgttac gtcgtggatg gacactgctt gcgaacgatc catgcagaga
    46381 tgaatgcact cctacagtgt gcaaaacttg gtatcccgac ggaaggttca gaaatctatg
    46441 tcacacattt tccatgtttg gcctgtacaa aggctttgtt gcaagctggt gtgaaaggga
    46501 tcaattatct ccatgactat cgaaatgatc cttatgccca agcgttgatc gaacaattag
    46561 gtgtatctgt acaccaagtt gctttagact cacagcattt ttcaaaactt caaaatgaat
    46621 taacaggaaa acatgtctag cgttttagaa aggcatgttc gaaatcggtt gatttttccg
    46681 gcaattacgt cgatgattat ctgtttgttg tattacactg acaattgggg ttatcgtttg
    46741 cttcttgccg gaattttttt atggttttcg ctaaaagaag gacgttttat cgcgtacttg
    46801 tgtattttgt gtagcataac ggtatccatt tcttataggc tacaagagta ttcattttta
    46861 gaggaaaaaa caacatctgc gcaaattact gtatttacag atacgattcg tgcaaatggt
    46921 aattttttga caatggaagg aaggttatca gagaataaac aaaagctcca gctttcttat
    46981 caagtaaaca cagaagcaga attgaaaaca tggcttaact tccgaggaaa gggaatagaa
    47041 ttgactgctg aaggcgagtt catctctcca gcaaaaaagc ggaatcttta taattttgac
    47101 caagaagctt acttcaggaa ccatagaatt tctggacggt tccaaattca aaagattgaa
    47161 cagatcacca tgcaaaatca ctggcgaaac tggcttcaga gaaaaagagg atcttttatc
    47221 tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat atattaattc tttattatgg
    47281 ggctataaag atgcaggatt cagagaacca gaagaaattt ttagggagag tggtttgctg
    47341 catctgttca gtctatcggg tctacacatc caattttatt tagggtggct gtattattta
    47401 tttcgacgta tcggatggcc actacacgtc agtattattc cgttgagtct actaactatc
    47461 tgttatgttt ctattgcagg aagttctatt tctgttttgc gtgctagttt gctgttttta
    47521 ttgcgattgc tagtcaaatt gctgaataag cattattcca ccatggactt atttgcagtt
    47581 gtcttttggc ttcttttact atttgatcct cgatttcttt ttcatgcggg tggacagctt
    47641 agttttttca tgagtttttt gtttatcctg atttcttttg atggagattg gttgaaaaaa
    47701 acggcctctc acgtcctgtt tactatgatc acgatgccgt tgattgtatg gcatttttat
    47761 gagtggccta ttttaggtag tctttttaca ttactgattg caccggtatt caagtttctt
    47821 tttcttcctg gtgtgttatt tctcacactc ttcaatcgct ggctgccgga aactttactt
    47881 ttgctgatag aagagggatt aatcttattt gagcgaataa tcgatttttc ttcagggtcg
    47941 tctctcatta taggacgttt gccccttgtg ctatctggga tgtatatttt cggattactg
    48001 atttgtatgg ataggttgaa agaacaacct gtgaaacact tgttttatgt tggtcttttt
    48061 tcttttattt ttttgacttt gccattttta cgcttttctt caacgatcgc ttttatcgat
    48121 gtcggtcaag gagacagtat ctttttgcaa agtccttttc gaaaagaaac gattttgatc
    48181 gatactggag gtcagttaca ctttgaaaga gaaaagtggg cgcaaggtat gatacgtcct
    48241 cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg ggataagtgt aatcgataaa
    48301 ctcatcttga cacacgacga tacggatcat gtgggagaat tacccacgtt atctcagcat
    48361 tttactatca aaaaaattta tatcggttgg ggcgctggac gaaatgagcc attacaaagg
    48421 catttatctg aagctcaaaa aaatggtact gagatcattg aaatcaaaca tggggacagg
    48481 atcagcggtt attatgatct ttatgtattg actccttttg aaaagggaga gggaagaaat
    48541 gaagattcga ttggtctatg gatggaatat aacaataggc gctttttatt tttaggagat
    48601 ttgaatcaag caatggaaaa acaattgctt ttgatttatc ctaatctgaa agccgatgta
    48661 gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca cagattttct ttcccatatt
    48721 gaagcaaaac atgggattat atcctgcggc gtaaataatc gttatggtca cccgcacagc
    48781 gaagtattgg agacactaga agaaaatcag ataaaaacat ggcgaacgga tcaacaagga
    48841 atgatcacct atcgctggca tccagttttt catcctcatg ggctagtgga aacaatgata
    48901 gactagtaat tctcatctgc tcatggtagg ataatttttg aaagtggtga gtaatttgag
    48961 tatacaagca gagttacaaa aaatacgtaa agaaaaactt gcatcttgtt acttgatttt
    49021 aggaacagaa aagtttctcc aagatcaagt tcggacagaa attgtcaaaa aaatccaatt
    49081 agagggcgaa gatgacttaa attttttaag ttttgacatg gaaaatgcaa gtttagatga
    49141 ggtagtagct gaagcagaga cgttgccgtt tttcggagat caacggttag tattcgtaga
    49201 gaatccttat tttttaacag gtgaaaaagt aaacaatgga atcgaacaaa atactgattt
    49261 attgacggct tatttgaaag aaccattaga atcgactgta ttagtattct ttgcgccata
    49321 tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg aaaaaaacag caattatcat
    49381 caatgtccaa cagttgaatg aaaaagaagt acgtcaatat ctgatgaata ccttagagaa
    49441 ttcctcaatc aaaatggaac gaagtgcaat tgatttattc ttgaggttga ccgacttaga
    49501 tttatccaaa atgatgagag agttacagaa attggtcctt tatggacaaa atcaacagca
    49561 aatcacggta agagaagtag aagaattagt tcctaaaacg ttagaacata atatttttga
    49621 catgacccaa tatattttaa aagggaagac ggaacaagct ttgcgtttat acgaagattt
    49681 agttatgcaa ggggaagaaa caatcaaaat taatgcgatt ttgctttctc aattacgttt
    49741 atttttacag acaaaatttc ttgtaaagat tggttatcag caggcaaata ttgctgaaac
    49801 attgaaaatc catccatatc gtgtaaaact tgccatgcag gaagtaaaaa aattcgatga
    49861 acatttgctg gtacagttgt ttgatcaatt agttgagatg gattatcaaa tcaaaacggg
    49921 acaaatcgaa aaagaattaa gttttcaact attcgttttg cgcacagggc agttgttaag
    49981 aaatcgataa gaagaaagac gagggcaaag catgcgggaa ggtcgtttgt ttttgtcctt
    50041 tttttgacaa ataaaaaaat cggccgaagc cgattctttt atttagcgat ttttttgctt
    50101 aaacgagatt tgtcgcggtt agctttgttt ttgtggatta gaccttttga ttcagccata
    50161 tcaatagctt taacagcttc tttatataac gcatctacgt tgtcagcacc tgaagctaca
    50221 gcatcttcaa attttttgat tgcagtacgc atagcgcttg tttgagatga atttttcaca
    50281 ttagcgtttt cgcttgtacg aacacgtttg attgcagatt caatgtttgg cattagtttc
    50341 acctccgata acttaagttc ttacgcgtgt taccgcataa ttcaacattc attattatac
    50401 ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt ttttccttaa cagtctttta
    50461 ttaggatttt ttttcagtaa aaacggatag tacaagctga aaaaaatcaa aaagacgcta
    50521 ttgtgggagg caatagcgtc aaaccaaact agatttttat ttatctattt aaacaaagtt
    50581 tgaatgggaa tgtttggtac taagttatta tataacaaag agaaccgttt gcaaagtttt
    50641 ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt ctattaagaa actgcagtta
    50701 ttttataacc agtatccttt gatcagtaaa ggctattctg gtgtaccgat tattttaatg
    50761 tattcatcga tattttttat caacattttt tttatgacca actggttccg acggttccat
    50821 ttgccactta cttccagcac atatttattt aggggcaaac gtaagatata ttttgccaaa
    50881 tagcgatctt taagcaggcc attgatcaca cggtcctcaa gctgcaagtt gatcccagtc
    50941 agcatcattg gctgttcttg taaaacgtac gtatcaatta aaagaccttc aaaagtttcc
    51001 acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag gcactaattc ttccattttg
    51061 atattcgttg aagtggatgc gatcattctt tcttttgtcc actgtaatct agtataacaa
    51121 aaaagagcag aagagccaaa aagaaaaaag agcatcgaat tctataggaa gtctcattgg
    51181 cacggcctaa attattacaa aacattgatt aaaaaaagcc cctgcatcgc aggagcatcc
    51241 cctatatcgt tttaataggt caatcaaaaa gcagtatcaa acacattttg tatcaaatca
    51301 ggaaataata gataagctaa gcccagaatc aaaagtaccg ctagaagaaa ccagccgatt
    51361 gttcctatga aatactttaa tttttcgaac attgtatcac ctcgttgtca gtatatcacg
    51421 gatcagtaaa ttgggggtgt taagaatctc ctaaaataga caaaatttgt tctattttcc
    51481 taaagcaaat acttgtaaga tacagtaagt gataccagaa gcaatgaccg gtccggcggc
    51541 gattcctctt aagaagacaa cacctaaaat cgtgccgaaa acaagtgcaa cagtgatttg
    51601 tggatcagaa gcaatcaatc ctacaccctt ggaagataag acagccacta gcccaccgca
    51661 aaaaacagct acccagccaa taggggattt aaaggcttct aatagatcac gtagaccgat
    51721 ttctcctgtt gcgataggga ttagaatagc cgctgtgatg atcgtcaccc caatattgat
    51781 tcctttgcct tgaagccaag ggaaaaagga acgggaggct ggtatgagct tcataatcaa
    51841 aacaaaagct gccgctatca tcagactttg atttttagct aaaattgcta caagtaaaat
    51901 aattcctaaa aaaagccaac tttccatgaa atatattcct cactttcttc gaaacaatca
    51961 taacatgttt ttagaaagtt acaaaagtag gaattcatcc ttcaagacct attcataagc
    52021 tgtttttttc ataaaataaa caaaatcata aaaaaacact ttgcttattt gacagaagac
    52081 agccgcgctt tgtatagtgg acaagactta catttttttg aagaatcaga aagatcaggt
    52141 aaaggtctta gtaaacgcaa gccattcaga ataaccaata ttgtgcttcc ttcgtgtcct
    52201 acaactccaa gaggtagatt taaaaattgc aggaagttag aagcaatcaa caagagaata
    52261 acaagaatcg aaaaaataat attttgtttg atgatccgcc gcatttttaa agatagccga
    52321 tgactgtaga caaatttatt taagtcgctt ttcattaaga cgatgtctgc gatatccata
    52381 gcggcatctg tcccttgccc catagcgaca cctaaagaag cggctgctaa agcaggcgca
    52441 tcattgactc catcaccaac catcgcattt acttggtaaa gttcattctc ttttttgact
    52501 agttgtgttt tttcttccgg agtacagctt gcatagtaat gttcaatccc tacttgttca
    52561 gcaatggcct ttgctgtgcc agaatggtcg ccggttatca tacttgtatg gatgttttgg
    52621 gatttaaagt aggaaattac ttgttgggta tttgcttttg gaatatctag cagacctaaa
    52681 acagcaatta actgctgatc tttagaaaga taaatcacgg ttttgccttg attttccaaa
    52741 cgttcgatca tttccgcaat ctctttgctg attatcatcg tctcaggatc atatgcgtgc
    52801 ttgccaactt tccattgcgc acctaaatag gtagtttgta atccaaaacc agtaatttct
    52861 tctattggta agtattggat ttcttcaggt attgtgcaat cgaaatattg caaaatcgca
    52921 tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa gaagttgttg tgccaaatcc
    52981 ttttcatcaa agaaaaagct gtcagttaca actggttttc ctttagtgag cgttcctgtt
    53041 ttatcaaagg caatcgcttt taattcagaa agcttttcta aatggatacc gcctttgatt
    53101 aaaatcccat tttttgcaga atttgaaaga gcagctagag aagctggtgt agcagatgcg
    53161 acaagcgcac aaggagaagc cacaactaat aataccattc ctcgataaaa gctttcatcc
    53221 caactccaac ctaggaaaaa gtgggggaga aggatcatta gaggaacagc aagcaaaatc
    53281 aattttacat aattattttc gattcgttcg ataaatgaag ctgtttgagt tggcgtattc
    53341 tgcgcttctt ctactaattg gataatctta gcaaagacag tttcggaact cgtttttgtt
    53401 actcgaatcg tgatgggatt tccttgattc agcgttcctg catacatctc atcgcctaaa
    53461 tatttttcta caggcacaga ttcaccgctt aatgcagact catcgatcgt tgcctgtgtg
    53521 ctttctaaaa agccatctat cggaacagct gctcctttag cgacaaatac caagttatcc
    53581 acctttagtt gagaaacatc tacttcttgc atttgtccat tttcttttaa taattgggct
    53641 ttttgtggtt gaagattcat caaagcagta atttcttttt gacttttatt agttgtgtat
    53701 tcctctaatg caccgcttaa acaaaagatg aatgtcaaca ttgctccttc aaagtaatta
    53761 ccgatcaaac aggcaccaat agcagctaaa gccatcaata aatccacatt cagtgtccga
    53821 tcattccaca attctttcaa gccttccgat gtttgtttat atccaccgat gataatggca
    53881 ctgatataaa aaagtggggt gatggtaaga gagaatcggt cgaagataaa accgaagatc
    53941 ataaataaaa ggcagccgat tgttgctgca aaagcttttt tctgattagt catcgttatt
    54001 agacctcctt gtcaaaacaa ggatagcata ataaaaatgt aattgataat aataacagct
    54061 aaatgagaac gataatggtt atcatttggt gattaagctt gatgttgttt ttattattta
    54121 taatgattat tattaaagaa aataaaaaac cagaacattt ctcaaataag agaagtgttc
    54181 tggttttttc tacattgcaa taaaccaata accaatcaaa atgataccaa ggataatacg
    54241 ataccagcca aaagcggtaa agtcgttttt cttcaaatag cttagtaaga atttgatagc
    54301 taagacggaa accacaaaag atacgaatgt tcctacaaga agaacaacag tttcttccat
    54361 tttgaatgaa ttcccttcag ctaaatattt gacgatcttc aagccgcttg cgccaaacat
    54421 gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca aaacgcgaag caccaatcaa
    54481 gatagatcct aggatcgttg cgccagaacg ggatgttccg ggaacaagtg ccaagacttg
    54541 gaataaccca acaagaagag ctgcttggta cgtaaagtca ttcagagaag tccaacgagg
    54601 tgttttgttt ttgttgcgtt tttcgacaat aataaaggca ataccgtaaa cgatcaaaac
    54661 aatggcaact ggtaagaact tgtggaaatt tgcttctaac caatcatcca aaggcaaacc
    54721 aatgatgatg gctggtatgc aagcaacgat cactttaaac caaagtgtcc acgtatcttt
    54781 tttttctacc gttgatttac ttggagaaaa tgggtttaac ttgtgaaaat aaagaacgac
    54841 gacagccaag atcgccccta attggataac gacattgaac atcgacataa attgtgaact
    54901 ggcatccagt ttgatgaatt catctgctag gatcaaatga ccagtactac tgatgggcag
    54961 ccattccgtt attccttcaa taataccgag aatcactgct ttgataatat ttgcaaaaaa
    55021 cataaattcc ttccttccca a
//
LOCUS       DAXWLH010000002        49293 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXWLH010000002 DAXWLH010000000
VERSION     DAXWLH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.002"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            141..512
                     /locus_tag="ACGE36_000051"
     CDS             141..512
                     /locus_tag="ACGE36_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFF8382485.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(786..1478)
                     /locus_tag="ACGE36_000052"
     CDS             complement(786..1478)
                     /locus_tag="ACGE36_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8382486.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1481..2266)
                     /locus_tag="ACGE36_000053"
     CDS             complement(1481..2266)
                     /locus_tag="ACGE36_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8382487.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2503..3903
                     /gene="lacG"
                     /locus_tag="ACGE36_000054"
     CDS             2503..3903
                     /gene="lacG"
                     /locus_tag="ACGE36_000054"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8382488.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3928..4245
                     /locus_tag="ACGE36_000055"
     CDS             3928..4245
                     /locus_tag="ACGE36_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8382489.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4258..5931
                     /locus_tag="ACGE36_000056"
     CDS             4258..5931
                     /locus_tag="ACGE36_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFF8382490.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5945..7576
                     /locus_tag="ACGE36_000057"
     CDS             5945..7576
                     /locus_tag="ACGE36_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFF8382491.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8067..9632)
                     /gene="guaA"
                     /locus_tag="ACGE36_000058"
     CDS             complement(8067..9632)
                     /gene="guaA"
                     /locus_tag="ACGE36_000058"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8382492.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9691..10614)
                     /gene="coaA"
                     /locus_tag="ACGE36_000059"
     CDS             complement(9691..10614)
                     /gene="coaA"
                     /locus_tag="ACGE36_000059"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFF8382493.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10810..11415
                     /locus_tag="ACGE36_000060"
     CDS             10810..11415
                     /locus_tag="ACGE36_000060"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8382494.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11723..12739
                     /locus_tag="ACGE36_000061"
     CDS             11723..12739
                     /locus_tag="ACGE36_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8382495.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12880..14181
                     /locus_tag="ACGE36_000062"
     CDS             12880..14181
                     /locus_tag="ACGE36_000062"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFF8382496.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14227..14889
                     /gene="deoC"
                     /locus_tag="ACGE36_000063"
     CDS             14227..14889
                     /gene="deoC"
                     /locus_tag="ACGE36_000063"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8382497.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14910..15308
                     /locus_tag="ACGE36_000064"
     CDS             14910..15308
                     /locus_tag="ACGE36_000064"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFF8382498.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15374..16456
                     /locus_tag="ACGE36_000065"
     CDS             15374..16456
                     /locus_tag="ACGE36_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8382499.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16668..18230
                     /locus_tag="ACGE36_000066"
     CDS             16668..18230
                     /locus_tag="ACGE36_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382500.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18223..19356
                     /locus_tag="ACGE36_000067"
     CDS             18223..19356
                     /locus_tag="ACGE36_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8382501.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19356..20312
                     /locus_tag="ACGE36_000068"
     CDS             19356..20312
                     /locus_tag="ACGE36_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8382502.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20411..21577
                     /gene="deoB"
                     /locus_tag="ACGE36_000069"
     CDS             20411..21577
                     /gene="deoB"
                     /locus_tag="ACGE36_000069"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFF8382503.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21601..22419
                     /locus_tag="ACGE36_000070"
     CDS             21601..22419
                     /locus_tag="ACGE36_000070"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8382504.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22436..23143
                     /gene="deoD"
                     /locus_tag="ACGE36_000071"
     CDS             22436..23143
                     /gene="deoD"
                     /locus_tag="ACGE36_000071"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8382505.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23441..24127)
                     /gene="gpmA"
                     /locus_tag="ACGE36_000072"
     CDS             complement(23441..24127)
                     /gene="gpmA"
                     /locus_tag="ACGE36_000072"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8382506.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24299..24880)
                     /locus_tag="ACGE36_000073"
     CDS             complement(24299..24880)
                     /locus_tag="ACGE36_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8382507.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25013..25696)
                     /gene="rpiA"
                     /locus_tag="ACGE36_000074"
     CDS             complement(25013..25696)
                     /gene="rpiA"
                     /locus_tag="ACGE36_000074"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFF8382508.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26015..26428
                     /gene="rpsL"
                     /locus_tag="ACGE36_000075"
     CDS             26015..26428
                     /gene="rpsL"
                     /locus_tag="ACGE36_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFF8382509.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26512..26982
                     /gene="rpsG"
                     /locus_tag="ACGE36_000076"
     CDS             26512..26982
                     /gene="rpsG"
                     /locus_tag="ACGE36_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFF8382510.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27076..29163
                     /gene="fusA"
                     /locus_tag="ACGE36_000077"
     CDS             27076..29163
                     /gene="fusA"
                     /locus_tag="ACGE36_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFF8382511.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29335..30522
                     /gene="tuf"
                     /locus_tag="ACGE36_000078"
     CDS             29335..30522
                     /gene="tuf"
                     /locus_tag="ACGE36_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8382512.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30929..31237
                     /gene="rpsJ"
                     /locus_tag="ACGE36_000079"
     CDS             30929..31237
                     /gene="rpsJ"
                     /locus_tag="ACGE36_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFF8382513.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31268..31897
                     /gene="rplC"
                     /locus_tag="ACGE36_000080"
     CDS             31268..31897
                     /gene="rplC"
                     /locus_tag="ACGE36_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFF8382514.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31925..32548
                     /gene="rplD"
                     /locus_tag="ACGE36_000081"
     CDS             31925..32548
                     /gene="rplD"
                     /locus_tag="ACGE36_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFF8382515.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32548..32838
                     /gene="rplW"
                     /locus_tag="ACGE36_000082"
     CDS             32548..32838
                     /gene="rplW"
                     /locus_tag="ACGE36_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFF8382516.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32877..33710
                     /gene="rplB"
                     /locus_tag="ACGE36_000083"
     CDS             32877..33710
                     /gene="rplB"
                     /locus_tag="ACGE36_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFF8382517.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33753..34031
                     /gene="rpsS"
                     /locus_tag="ACGE36_000084"
     CDS             33753..34031
                     /gene="rpsS"
                     /locus_tag="ACGE36_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFF8382518.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34054..34401
                     /gene="rplV"
                     /locus_tag="ACGE36_000085"
     CDS             34054..34401
                     /gene="rplV"
                     /locus_tag="ACGE36_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFF8382519.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34415..35071
                     /gene="rpsC"
                     /locus_tag="ACGE36_000086"
     CDS             34415..35071
                     /gene="rpsC"
                     /locus_tag="ACGE36_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFF8382520.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35074..35508
                     /gene="rplP"
                     /locus_tag="ACGE36_000087"
     CDS             35074..35508
                     /gene="rplP"
                     /locus_tag="ACGE36_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFF8382521.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35498..35686
                     /gene="rpmC"
                     /locus_tag="ACGE36_000088"
     CDS             35498..35686
                     /gene="rpmC"
                     /locus_tag="ACGE36_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFF8382522.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35711..35977
                     /gene="rpsQ"
                     /locus_tag="ACGE36_000089"
     CDS             35711..35977
                     /gene="rpsQ"
                     /locus_tag="ACGE36_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFF8382523.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36035..36403
                     /gene="rplN"
                     /locus_tag="ACGE36_000090"
     CDS             36035..36403
                     /gene="rplN"
                     /locus_tag="ACGE36_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFF8382524.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36440..36748
                     /gene="rplX"
                     /locus_tag="ACGE36_000091"
     CDS             36440..36748
                     /gene="rplX"
                     /locus_tag="ACGE36_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFF8382525.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36776..37315
                     /gene="rplE"
                     /locus_tag="ACGE36_000092"
     CDS             36776..37315
                     /gene="rplE"
                     /locus_tag="ACGE36_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFF8382526.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37334..37519
                     /locus_tag="ACGE36_000093"
     CDS             37334..37519
                     /locus_tag="ACGE36_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFF8382527.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37555..37953
                     /gene="rpsH"
                     /locus_tag="ACGE36_000094"
     CDS             37555..37953
                     /gene="rpsH"
                     /locus_tag="ACGE36_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFF8382528.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37985..38521
                     /gene="rplF"
                     /locus_tag="ACGE36_000095"
     CDS             37985..38521
                     /gene="rplF"
                     /locus_tag="ACGE36_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFF8382529.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38682..39038
                     /gene="rplR"
                     /locus_tag="ACGE36_000096"
     CDS             38682..39038
                     /gene="rplR"
                     /locus_tag="ACGE36_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFF8382530.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39059..39559
                     /gene="rpsE"
                     /locus_tag="ACGE36_000097"
     CDS             39059..39559
                     /gene="rpsE"
                     /locus_tag="ACGE36_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFF8382531.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39574..39753
                     /gene="rpmD"
                     /locus_tag="ACGE36_000098"
     CDS             39574..39753
                     /gene="rpmD"
                     /locus_tag="ACGE36_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFF8382532.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39797..40237
                     /gene="rplO"
                     /locus_tag="ACGE36_000099"
     CDS             39797..40237
                     /gene="rplO"
                     /locus_tag="ACGE36_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFF8382533.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40237..41532
                     /gene="secY"
                     /locus_tag="ACGE36_000100"
     CDS             40237..41532
                     /gene="secY"
                     /locus_tag="ACGE36_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFF8382534.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41594..42241
                     /locus_tag="ACGE36_000101"
     CDS             41594..42241
                     /locus_tag="ACGE36_000101"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFF8382535.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42432..42650
                     /gene="infA"
                     /locus_tag="ACGE36_000102"
     CDS             42432..42650
                     /gene="infA"
                     /locus_tag="ACGE36_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFF8382536.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42683..42799
                     /gene="rpmJ"
                     /locus_tag="ACGE36_000103"
     CDS             42683..42799
                     /gene="rpmJ"
                     /locus_tag="ACGE36_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFF8382537.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42817..43182
                     /gene="rpsM"
                     /locus_tag="ACGE36_000104"
     CDS             42817..43182
                     /gene="rpsM"
                     /locus_tag="ACGE36_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFF8382538.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43210..43599
                     /gene="rpsK"
                     /locus_tag="ACGE36_000105"
     CDS             43210..43599
                     /gene="rpsK"
                     /locus_tag="ACGE36_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFF8382539.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43679..44617
                     /locus_tag="ACGE36_000106"
     CDS             43679..44617
                     /locus_tag="ACGE36_000106"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFF8382540.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44645..45025
                     /gene="rplQ"
                     /locus_tag="ACGE36_000107"
     CDS             44645..45025
                     /gene="rplQ"
                     /locus_tag="ACGE36_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFF8382541.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45297..46136
                     /locus_tag="ACGE36_000108"
     CDS             45297..46136
                     /locus_tag="ACGE36_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8382542.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46112..46981
                     /locus_tag="ACGE36_000109"
     CDS             46112..46981
                     /locus_tag="ACGE36_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8382543.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46971..47768
                     /locus_tag="ACGE36_000110"
     CDS             46971..47768
                     /locus_tag="ACGE36_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8382544.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47971..48717
                     /gene="truA"
                     /locus_tag="ACGE36_000111"
     CDS             47971..48717
                     /gene="truA"
                     /locus_tag="ACGE36_000111"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8382545.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49046..>49293)
                     /locus_tag="ACGE36_000112"
     CDS             complement(49046..>49293)
                     /locus_tag="ACGE36_000112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382546.1"
                     /translation="KMTNLFRLSSLRAIKTTFHRFLVTEKIGNKKMIHLSTPRPQVTI
                     FLKDEKLFFEIVTCDSELNDRLTTSFLNGVQSADLRY"
BASE COUNT        15605 a         8998 c        10814 g        13876 t
ORIGIN      
        1 tcaacactca gagaacgggt tggtttttaa tagatttaat aagtactgat aatgaaattt
       61 ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga attttttcaa gattactttg
      121 cggagtatgc cgataggata atgagagatt acgtaacaga taaaactcaa gaagtcatca
      181 atcaatccca gtcattaaat gaattatctg ataaggcgaa agaagaagca gttgccatta
      241 tttcttcagg gattagtgac tatattgata tgagtggtga aatcaattat ggcgataaag
      301 acaaaattgt acgcatgata aaagctttta tcaaaacttt tgatgtgaag gcagatctta
      361 agtttaatga tacttaccta gtaggaaaat taattaatat ccttgggaca gagaaagata
      421 cggaaacgtt tatttcctct ttgtctatgt tgtttacaga taagcatttt tcaactcagc
      481 ttggtggaga ggaactagtg gaaatctttt aaccattacg cccagcatta ataaaagtat
      541 atcaaaaaag tactcctgat gatttttaag attggtttga gaaataatgt gaaaacacta
      601 tttttaatgt taattataga tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt
      661 caaagataca ctgctatatt tcactaagga tatagcagtg tatctttcat gtcgtttcac
      721 tggcagtttt ttataaaagg aggtgtgcat ttcagttgct tttctttggt agttagttga
      781 atcgtttaag aattaacgga tgtggcagac aggtgctctg tccaaaaacc agtaaaatct
      841 ttgatggttc catctttatt tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat
      901 ggatcaattg caataattgt tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa
      961 gcatcttcga taacaagaca atcagccgga gctaagctca gtttgtctgc cgctttaaga
     1021 aaaatatccg gttgcggttt cccagggaaa gagccgtcgt catagacaat cttgtcccag
     1081 tcaaaccagc gggctaaatc gaaaatatca aaataaaaag caacattctc tttgacagta
     1141 gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc
     1201 ccttttgtca actgaagctc gttttcattt tgcaagcaaa gcatacgata atagctttct
     1261 ttttcttctg acaatacggc gatctcagag gcggtcaaat gctctgaaat gaaatgtttc
     1321 aaaatctccg tattcgtccg accatgaata ttttttaaaa tatcctcgtc tgataaacca
     1381 tcttttgtat gatcatgtat catatgcagc caagcagctt catgcagatg agagtccaaa
     1441 aacattgttc cgttgaaatc aaatataatc cctttcatag ttacgctcct atagtggtat
     1501 tttcggttga atcagcaatt tgcagatcga catattgtgc atagtgctct tgaacctccc
     1561 gcgagatctg gcgatccgta atgagcagat cgaaatcatg caaattataa aacacataaa
     1621 aatcatctcg attgaatttt ttattatcaa cgagtagata tttcttacgg gaattattta
     1681 gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa
     1741 cgccatttga actgataaat gcttttgtaa actttagtct tcttaaattg tcgttggtga
     1801 tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt agaatttctg
     1861 gtccatctgc ttgagcaaga atatcaaaaa ctggataatt atttgtaatg atccgaattt
     1921 tcttgttcag taaatgcgtc gccaatagtt cgattgttgt acctggtcct aagaaaatcg
     1981 tttctccctc gtgaatcaag gaggctgctg tggcagcaat ttcttttttt tcttgaatct
     2041 gcagagtggc tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa agactttgag
     2101 cccctccatg gacacgcagc agcttgcccg atttttctaa ttcatctaaa tctctgcgaa
     2161 cggtcatatc agaaacatct aattgattga tgatttcatt aacggtaagg attccttttt
     2221 tattgaccat ttctacgata gtaagcaatc tttctttttt caacattgtc ttcacctcta
     2281 atttaaggat aatctatcat ataaacggac aacagtcaat ctaattcgaa caaaagtaaa
     2341 catttagggt ggtttttatg tttgttacaa tagattttat tgctgttttc tggtttttat
     2401 gattatttgt gtttgttttt gttgacaaat gttttggatg cgtttacaat gcaatcagtt
     2461 agaaagagcg aaaactgatt atagatttga ggagagatgt acatgcttaa attaccagaa
     2521 gattttattt ttggcggagc cacagcagcc tatcaggttg aaggagccac taaggaaggt
     2581 ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac aagggcgatt cagtcccgat
     2641 ccggcaagtg atttttatca tcaatatgca aaggacattg aattgtgtga acgttttggt
     2701 gtaaatggtc taagattatc cattgcttgg agccgcattt ttcctgatgg agctgggaaa
     2761 ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac
     2821 gtaacgccgt ttgtgacgtt gcatcatttt gataccccta aacgtttgtt tgaccatgga
     2881 gattttttga atcgagaaac aatagaagca tttgtttcct atgccatttt ttgttttcat
     2941 gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa tctatccagt tgcaaccaat
     3001 caatatttgt taggtgtttt cccaccagga atcaaatatg attttacaaa gatcgtcgcc
     3061 tgtcttcata atatgatggt tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa
     3121 cttccgggtg aaattggtgt agttcactca ttagagacga aatatgcggc aacagacgcg
     3181 ccggaagata agcatgctgc ctttttagac gatgcattgt cgatccgttt cttattagat
     3241 gctacctatt taggctatta ttcaactgaa accttgactg ctcttgatga gatatgtgaa
     3301 gcaaatcagg ccagctatca ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc
     3361 acgcgtaatg actacttagg gatcaatcac taccaatgcc attttgtcaa agcttacgat
     3421 ggagaaaatg caatccatca taacggtacg ggtgaaaaag ggacctctgt ttataaagtg
     3481 aagggaatcg gagaacgtat ttataaagaa ggaattcctc gaactgattg ggactggctg
     3541 atttatccag aaggtctata tgacttgttg ctgcgaatta aaagcgatta cccacattac
     3601 aacaaaatct atatcactga gaacgggatg gggtataaag atcagtttga agatggcatt
     3661 attatggatc agccgagaat cgactatttg agagtctatt tggaatcctt aagcaaggcg
     3721 ataacagccg gggtaaatgt gaaggggtat tttttgtggt cgcttatgga tcttttctca
     3781 tggacaaatg ggtataacaa aaggtatggc ttgttttatg ttgattttga aacccagaag
     3841 cgttacccaa aagaatcagc ttactggtac aagttggtca gtgaaaccaa aacgattatt
     3901 taataagtga taatggagga agaaaagatg aataaagagg aattacaaat gcttggtttt
     3961 gagatcgttg catattcagg cgacgctcgc agtacattat tgaaattatt gaaagaagta
     4021 agacaaggaa actttgaaca tgttgattcg gcgttgaaag aagccgacga aaatttaacc
     4081 cttgcacata attcgcaaac aaaaatttta gcggaagaag catctgggaa agagatggag
     4141 atgggattca tttttatcca tgggcaggat catttaatga ccactttatt gctgagagat
     4201 ctgattcaag actttattgt actgtacaga cagaatcaag gataggagaa gaacgctatg
     4261 gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat
     4321 agttatttag gagccgttcg tgacggattt ttaacagcga tgccagctat tttattttca
     4381 agcattttca ttttgattgc tgcggttcct gagatttttg gttaccaatg gcctgaatct
     4441 gtctccacat ggctatggaa agtctataat tattcgatgg gtgttgtagg gttactcgtt
     4501 tcagcaacca ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa
     4561 gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc
     4621 tctccgattg aaggtggatt ttcaacggaa tatatgggga caaagggact actggcttcc
     4681 tttgtcgctg catttttaac agttaattta tataagtttt gtgtgctgaa agatattacc
     4741 attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga tatttttcct
     4801 ttctcttttt cagtatttgc ggccgtttta attgatttag gcattcgaca aatcttccaa
     4861 atctcatttg cagaagcaat cattacggtg ctgcagccgc tatttacagc agctgacggg
     4921 tacttaggaa tcgctattat ctggggagcg atggcgctgt tttggtttgt cggtgttcat
     4981 ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct atgccaatgt ggaaaccaat
     5041 ctgcaattgt tcaattccgg tgaacaagct tctaaagtac tgacggtggg tttagggaat
     5101 tttgtaggaa ctatgggagg aactggggca acattggttg ttccttttat cttcctactc
     5161 tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt cctttattcc agtaagtttc
     5221 gctgtcaacg aaccattgct atttgcggca cccatgattt tgaaccctta tttttttatt
     5281 ccatttttgt taacacctgt tgtaaacgtt tgcctgttca agttttttgt cgatgtattg
     5341 ggcatgaaca gctttatgta cgttttgcct tgggcaactc cagcaccgat cggtttggtt
     5401 ctaggaacgg gcatgggtat cttagctttt gttctagcag cggttctaat cgtcgttgac
     5461 ttcctgatct atctaccatt ttgcaaagca tacgatgctg tcttagtaga gaaagaagca
     5521 caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta
     5581 cctgtggcag cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt gttggtactt
     5641 tgtgctggcg cagggacaag tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa
     5701 tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt ctcattacga aatgctgcag
     5761 gattttgata tggtggtctt agcaccgcaa gtcaattctt actatgatga tatcaaagaa
     5821 gacaccgatc gcttaggaat caagctggtt gcgacaaaag gagcagaata tatcggatta
     5881 acgagagatc ctgaaaaagc tgttcagttt attctgaacc aatttcaata aaggaggagc
     5941 cataatgaaa atgggaattt ggttgcgtaa agctgtttta tttgcagctg tgttaggggc
     6001 tttgggggct gtttcaaaaa ctgttgaagc atctgccagc gaatttattc gcggagcaga
     6061 tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag acgatatttc
     6121 aaaagatgcc ttagagattt taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg
     6181 gcaggatcct tataatagta atggagaaag ttatggagca gggacaaatg atctggaaac
     6241 gacgattgac cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc tggattttca
     6301 ctacagtgat ttttgggttg atcctgggaa acagaatctt cctaaagcat ggcaaggtct
     6361 gacctttgaa gaaatgaata cggcattgta cgactataca aagaacgttc tgtcggaaat
     6421 gaagcagctg gacgtttatc ctgatatggt tcagatcggc aatgaactga atagcggaat
     6481 gctctggcct tatggcaagt cttggggaga aggcggggga gaatttgatc gattggctgc
     6541 ttttttgaag tctggtatcc aagctgttcg agacactcaa cctaaaacaa ctccagtaat
     6601 gctccactta gctgatggtg gcgatacagg agcatttact tggtggtttg atgaaatcac
     6661 gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat tatccgtatt ggcacggttc
     6721 aatgaatgaa cttcaaacca atatgaatgc gatcagccaa cggtatggta aagaagtgat
     6781 cgtagttgaa acagcttatg ggcatacaac agcgaatgcc gatactatgc caaatgcttt
     6841 tggtgaagcc gaagcagctg cggggggata tgaaccaaca ccgacaggac aagcagagta
     6901 tttgctggat cttgccgatc ggattcaggc agtaccgaat aatcgcgggg cgggcttctt
     6961 ctattgggaa cctctttggt acaacggaaa tgtgagttgg gcaacacaag caggtatgaa
     7021 ttatctcagt gttcaatcca ctatgggaaa cgaatgggac aaccaagcga tgtttgattt
     7081 tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct
     7141 agttaaaaat gcaagtttcg aaagcgatgg accaacgacc agtccttcca attggcaaac
     7201 ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg gaagcgggca ctttttttgg
     7261 ggactacaaa ctaacttttt ggcaggatca agcatacgag gcatcagctt atcaaaatgt
     7321 tagtggcata agcaaaggaa cttacacatt gagtgcctat gtaatgtctg ggggagagtt
     7381 tgatactaat cagttatata tcaaaaatta tggtggagcg gaactaaata ctgcggttgt
     7441 taaaacaagt gaatggacaa aaattacaat caaagacatt ccaattacaa caggtgtttg
     7501 tgaaattggg gtttatagca aggcttcagg aaatgcttgg acgaacgttg attttgtttc
     7561 attggtgaaa caatagactg cggatcgttt cgattcaaga tcggaggttc ccttcagcca
     7621 catcgttagc gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa aaataaccag
     7681 aaacgatttc cagtcatttt taccaaaacg agtaaagcac cagacttcat ctacactttg
     7741 tattatgcta aagagagttc aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt
     7801 gtgcagataa cataaagttt gtttaagaaa gggtaacctt agaattccgt cgtgaatatg
     7861 atttgagcac ttagatggat agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact
     7921 tctttttatg ataagaatga aacaaattaa cttatattat agtctctttt ggtagggaac
     7981 catcttattt ttaatgaaaa tcaagcattt tagctattga agaatgttga tttataggga
     8041 ttccccctcc atattatgtt ggctctttat tcccactcca ctgttgctgg tggtttagaa
     8101 gtgatgtcat acacgatacg gttgacgtgg gctacttcgt ttacgatacg tacagagatt
     8161 ttttgcaaga cttcccatgg gatacgggca aagtcagcgg tcattccgtc gattgaagtt
     8221 acagcacgga taccgactgt gtagtcgtaa gtacgtccgt cacccataac accgacagag
     8281 cggatccctg gaagaacagt gaaatattgc cagatatcac ggtcaagtcc agcagctgcg
     8341 atttcttcac gcaagatagc atcagattcg cgtacgattt ctagcttgtc ttcagtgatt
     8401 tcgccaagaa cacgaatacc aagaccagga ccagggaatg gctgacgcca aacgattgaa
     8461 tcaggcatgc ctaactgagt ccctaattca cggacttcgt ctttgaataa tgtgtttaat
     8521 ggttcgatca attcaaattg catatcttct ggcagtccac ctacattatg gtgagatttg
     8581 atcgtctgtg ctgtttctgt accagattcg ataacgtcag tatacaacgt tccttgtgct
     8641 aggaaagaga cgccttcttc accagcaagt tttgttgctt cgtcatcaaa aacatatacg
     8701 aattcattcc caatgatttt acgtttttgt tctgggtcag atacaccttt caacttgtct
     8761 aagaagcgat ctcttgcatt gactttgatg atgttcaagc cgaattttcc gcctagactt
     8821 tccattactt gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc
     8881 aattggtcac cgatcgcttt ttgtaaaaga accccaacga cactagaatc aacgccacct
     8941 gaaagaccaa gcaagacttt tttgtcacca acttgttcac ggattttttc tacttgcata
     9001 tcgataaagt tatccattgt ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg
     9061 cgcagcaatt cgattccgta ttcggaatga cgtacttctg catggaattg gataccataa
     9121 aaattacgtg ttgtgtcttg gatagaagca atcggacaat ctttgcttgt tccgactgtt
     9181 tcaaagcctt caggaacttt agttaccaag tcaccatggc tcatccatac tgtttgttgt
     9241 tttggtgttt ctttaaacaa gacagcttga tcgcttgtta cttctaacaa ggctttccca
     9301 tattcgcggt ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat caattgcata
     9361 ccatagcaaa tccctaatac aggaatacct aaattgaaga tctcaggatc gatcccaaat
     9421 gcattctcat catatacact gtttggtccg ccagaaagaa tgattccctt tggattcatc
     9481 gcttttactt cttctgcagt aatgcggtgg ctcaataatt cggaaaagac tccaagatca
     9541 cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt agtctaatac gataatcttt
     9601 tcaacgttcg tcatatcggc aacgtttgtc aaatttatta cccctatccc tttaaatttt
     9661 atctcaaaat agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat
     9721 gatatgattt tcagttttat ggagaattat atcggctctt cctcgtgtag gcaagatata
     9781 ttcctgtaaa tttttcaaat tcacattttt ccatacatct tttgccattt taaaggcttc
     9841 ttcacgatct ccgatagcaa aacgataata ataattctct ggattttgga aagccgtatc
     9901 cagtaacgca ccaaaacgat ccaaatacca tttttcgatc agcacgggat cggcatcgac
     9961 atagacagaa aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag gtaattgaag
    10021 cgtattgatc ccctcaacga tcaagatatc aggctgctgg atcaattcgt acttgtctgg
    10081 aatgatatca tacacatcat gtgagtaaac aggtactttt atctcttcct gaccagattt
    10141 tacacggttc aagaaatcga tcagcatctc catatcataa ctttcaggaa atcctttacg
    10201 gtccatgatt ccacgctctt ttaatatacg atttggataa agaaatccgt cagtcgtgat
    10261 cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca gtctggctgt
    10321 tgtacttttt ccaacggcta cacttccggc gatcccgata ataaaaggcg gtacttctac
    10381 gtatttatga agaaataaac ctttactcaa gaacaaagat tcaaattctt tcatataaag
    10441 ataaatcaga tgagcaagag gtacatagat atctcgaaca tcctgcatgg aaatgcggtc
    10501 attcaaactt ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata
    10561 aaattctcgc cattcttctc ttgccacacg gtagtagttc attggatcat tcatattagc
    10621 ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa accccaccac
    10681 ttaagagaga ttgccgatct ttttgtataa tctttataat agaaagaaac ggagtcttat
    10741 tcttgacttg cgcacggata cataaaagga tagactgttg tcatgaaaga aatgagaagg
    10801 atggaagaca tgacaaatca ttactattct gaacgcccag atacggcgca cgattttgaa
    10861 caatgggctt ttgagttaaa aggcagaacc tttcaatttg taacagattc tggtgtattt
    10921 tcacgtgaaa cagtggatta tggttcacgt gtcctgattg atgctttcga ttgggaagag
    10981 ttacctgatg aaggaaagat cttagatgtg ggatgcgggt atggaccgat tgggttagct
    11041 ttggcatttg cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt
    11101 ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat
    11161 atctatgaag cagttcatga agaaacctat gcagctatcg taagcaatcc gccgatccgt
    11221 gctggaaaaa aagttgtaca tgaaattttg acaggcgcat atcctcggtt gaagaagggc
    11281 ggtacactga caattgtgat ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg
    11341 gaagaaacat ttggtaatgc agaaatcgtc acaaaagata aaggatatta tatcatcaaa
    11401 agtgtgaaag aataactagc tgaaaaatag tggagcgacg aaagagtgga actgggcgtg
    11461 catgtacggg tacgtatcag ttcttttttt gttgagaagt ttttaaaaac aaagaactat
    11521 tttttcttca aaaagaaata gtaggaacag agtcgttgtt ttttacgata aaataaataa
    11581 gatacagaac ttgtatagaa ggctgccaag tcggtagata aaggaggttt ttatcaaaaa
    11641 acatactttc cttttttctc tattcttttc ttgggggttt tggtacactg atagggaatt
    11701 tagatagaag ggagccaata ggatgaagct gaccatcaga gatatcgcag aaatggcagg
    11761 cgtatcagta acaactgtct cacagatttt gaataacaaa ggaagcagat ttagtgataa
    11821 gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg attatttcgc
    11881 ttcaaatgtg atcaatcgac attcaaagac catcggaatg atcgtccctg atgtcactga
    11941 tttcttcttc tccaaactga tcgaaggtgt agagagctat ttgaatcctt tgggctatgt
    12001 gatcatgtta tgcaattctc gtcatagcca agaaaatgag atcaagtatt tacaagagct
    12061 ttcccaccgc tcagtggacg gaatcttgct ggccacacca aatattttac ctgagaaata
    12121 tgcattagac agcggttttt atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa
    12181 tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc
    12241 gctgtttatt gaaaatggcc atacgaagat cggcatgctg aaggaaacca ctggatacta
    12301 tcaattagaa gaacgtttca acggatatcg tcatgcgctg aaagatcatg gcctgccttt
    12361 taatgggaaa tttgttgaac atggagagtt gactgtccaa ggtggatata ctgcatcaaa
    12421 aaaattactc aaacataaag atatcacggc tattttttgc gggaatgatg ccatggcgat
    12481 cggatgctat caggcgatcg atgaattggg caaaaaaatc ccagaagata tctcagtcat
    12541 aggattcgat ggattaaagc tgtcagaata catgattccg caattgacga cagtgcagca
    12601 gccaagtttt gatattggat tttatgctgc acgtttcctg atcgatacga tcgaatttcc
    12661 tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta attattcgtg agagcgtaaa
    12721 agtattgaca aagggctaac tcggcggtat attgcttatg taagcattta caaaggctag
    12781 ttagtgcttt ttttattttt agtaaaccag tttactaaag tggtttacct ttcctaaaaa
    12841 taatgtataa taccgaatga agacaaggag gatcaggcaa tgcgaatggt ggatctgatt
    12901 gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac
    12961 tatacgaatg gaaagattcc agattatcaa gtcagtgcat tattgatggc aattttttat
    13021 caagacatga caaatgaaga aatcacgaac ttaacattgg cgattgctaa ttctggtgat
    13081 gtcatcgatt tgagttctct tgaaggaata aaggtagata agcattcgac aggtggcgtt
    13141 ggtgacacga ccacattgat tttagccccc ctagtagcta gtgtcggagt gacagtggct
    13201 aaaatgtctg gacgcggtct aggttacaca ggtggtacac tagacaagct agaagcaatt
    13261 cccggatttc agatcgagct ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa
    13321 gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg
    13381 cgtgacgtca cagcgaccgt tgattctctt ccgctgattg ccagttcgat tatgagtaaa
    13441 aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga ccactggaga cggtgctttc
    13501 atgaagaaca tcgaagatgc aagacgtctg gcaaagacca tgacgagcat tgggaaactg
    13561 gctaaccgag aaacagtggc ggttatctca gatatgtcag aacctcttgg cgaagctatc
    13621 ggaaatagtc tagaagttgt cgaggcaatc gaaacattac aaggaaatgg tccagaagat
    13681 ctagtagaaa tgtgctacgc cttaggaagc caaatggttg ttcttgctgg caaagctaag
    13741 acaaccgatg aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa agcgttagca
    13801 aaattcaaag aaatgatcca aaatcaaggg ggagacccag cgatcgtgga acatccagaa
    13861 cgaattttga ctgcacgcta cacaatggag cttcctgcta aacaaagcgg agttgtgtca
    13921 aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc
    13981 aaagaagaag atattgatca cgctgttgga ttgaaactgc ataaaaaaat tggtgacact
    14041 gtgacaaaag gtgagtcact attgacgatc tatagtaacg acgaagaaat cacttctgtc
    14101 attgaattat tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt
    14161 catgacatca tcacagaata aaggaaaaaa gcggactttg ccgttttaaa gaaaagggga
    14221 aaagaaatgg aactaaaccg aatgatcgac catactattt tgaaagcaga cgcatctaaa
    14281 gaagctgtga tgcaaattat cgaagaagca aaaaaatacc atttttattc tgtctgcatc
    14341 aatccagcat gggttgcgct agcgaaagaa caattacaag gtgagccagt agccgtatgt
    14401 acagtaatcg ggtttcctct aggagcaaac acacccgaga caaaagcatt tgaaacaaca
    14461 gatgccatca ataacggtgc agatgaagtc gatatggtca tcaatatcgg tgctttgaaa
    14521 tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat
    14581 cgtgcgcttg tcaaagttat tattgaaaca tcgttattag atagagaaga aatcatccga
    14641 gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca aaacatcaac tggtttctca
    14701 acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg
    14761 ggtgtcaaag cttccggcgg tatccataac gaagaagaag caatggcaat gatcgaagct
    14821 ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg tatctggttc aactggtgaa
    14881 ggctattaat tttttcaaag gagaagctca tggcagaact caaacaagaa tggatcaaca
    14941 cagctattga agcgttagat aaagcctatg ttccttattc ccattttccg gtaggcgctt
    15001 gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa cattgagaat gcatcatttg
    15061 gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa
    15121 catttacgca tctagtcgta gcggggcaca cccccgatcc tatctctcct tgtggtgcat
    15181 gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc ggttacgcta gtaggagaca
    15241 atggtgtgac gaaggcaacg accgttcgtg cactgctgcc ttatgcattt actgaaaaag
    15301 atctataact catgttcatt cagtcagctg actgttgaaa aataaaacaa ttattttagg
    15361 aggctttaca gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg cactagcagc
    15421 agggttattt ttaggtgcat gcggcaacaa cggctctact gattcatccg gtggcaaaag
    15481 ctcatcagac acaacaactg cagcattgat cacagacaca ggtggtgtag acgaccgttc
    15541 attcaaccag tcagcttggg aaggactaga aaaatggggg aaagatcaag gactttcacg
    15601 aggaaatgat ggcttccaat atttccaatc ttcaaatgaa tcagattaca tcccaaatat
    15661 tgaccaagca ttgaatgctg gattcaaaac gatttttggt attggttata aactaaaacc
    15721 agcgatccaa gaacaagcaa caaacaatac aggtacgaac tttgttatta tcgatgatgt
    15781 catcgatgga ttagataacg ttgtatctgc aactttcaaa gacaacgaag cttcttatct
    15841 tgcaggtgtt gcagcagctt atacgactga aacaaacgta gtcggcttta tcggcggtgt
    15901 aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa gccggtgttg atgcaggtgc
    15961 gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact tctctgctcc
    16021 agataaagga cgttcaattg ctcaaggtat gtatgcacaa aacgcagata tcatcttcca
    16081 tgcttcaggc ggtacaggta acggtgtatt ccaagaagca aaatcattga acgaatcagg
    16141 cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac
    16201 attaaacggc gaaaagaaaa acttcacatt gacatctaca ctgaaagcag ttggaacagt
    16261 agtagaagat ttggcacaaa aatcagcgga cggtaaattc cctggcggcg aacacactgt
    16321 ttatggattg aaagaagacg gtgttggtct aacagaagga caactttctg acgaagcgaa
    16381 aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag ttccagaaac
    16441 tcctgaagaa aactaaaacc atttctatag ccttgttaga cacatgtttg acaaggctat
    16501 ggtttgatta gaacaagaat tgttcattta tccaagctgc cttgtgacag tttggataag
    16561 ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct
    16621 tagtcaatcg ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa
    16681 atattgtcat agaaatgcag aatattacca aagaatttgg aacattcaag gcaaatgaca
    16741 acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg
    16801 gcaaatctac attgatgaac gtgctttccg gattattaga gccgacatca gggaaaattt
    16861 tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa agccaaccaa ttagggattg
    16921 ggatggtcca tcagcacttt atgcttgttg atgcctttac tgtaacagaa aacatcgtgt
    16981 tgggaagcga gcctagtcgc gcagggatgc ttgaccataa aaaagcacga aaagagatcc
    17041 aaaaagtttc tgaacagtat ggattgtcag tcaacccaga tgcttacgtt cgtgatattt
    17101 cagttggaat ggaacaacgg gtagaaattt taaaaacact ttaccgagga gcagatgtac
    17161 tgatttttga tgagccgaca gctgtattga cccctcaaga aattgatgaa ttaatcgtga
    17221 tcatgaagga attagtcaaa gaaggcaagt cgatcatttt gattacgcat aagttagatg
    17281 aaatcaaagc agtagctgac cgctgtacag ttatccgccg tggaaaagga atcggtacag
    17341 tcaacgttaa agacgtaacc tcacaacaat tagccgatat gatggttgga agaacggttt
    17401 cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc
    17461 tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg
    17521 ctggcgaagt acttggtatc gctggaatcg atggaaacgg gcagtcggag ttgatccaag
    17581 ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg gaagacatca
    17641 ctaataaaaa accccgaaaa atcactgaac atggtgtagg acacgtgcca gaagaccgtc
    17701 ataaatacgg gttagtccta gatatgacat tatctgaaaa tatcgcacta caaacctatc
    17761 atcaaaaacc ttacagtaaa aacggcatgc tgaattattc agtaatgaat gaacatgcca
    17821 gagaattgat cgaagaatat gatgttcgaa caacgaatga acttgttccc gcaaaagctt
    17881 tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga gatcctgatc
    17941 tgttgattgt tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa tttattcata
    18001 aacgtctgat cgaacaaagg gacaaataca aagcagtgtt actgatcagt ttcgaattag
    18061 aagaaatttt aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa attgtcggta
    18121 tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct attgatggcg ggctatacct
    18181 tagaagaagc acgaaaagag ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa
    18241 aaattccgta atttattagt gcctgtactt tcagttgttt taggattgat cttaggcgcg
    18301 atattgatgg ctgcttttgg ttttcagccc atccaaggtt attcttctat gctagatgca
    18361 gcccttggtt cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc gttgattttt
    18421 acagcattag gtttttctgt agctaactca gctgggtttt tcaatattgg gctttctgga
    18481 caagcacttt gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca
    18541 aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg
    18601 gctgttccag gtttacttcg agcatttttt ggaacaagtg aagtgatcgt aacaatcatg
    18661 atgaactata tcttattata cgtaagcaca tttgctttac atgaagtgat gcctgaaagc
    18721 tatcgctcaa gtttggactc ttccaatatg atcagtcaaa atgcctcact gcggttacca
    18781 tggttaacac aagcttttgg cggctctcgc gtaaactcag gattattttt agcaatcatc
    18841 gcgcttattt tcgtttggct gctgatgaag aaaacgactt taggatttga aatccgttct
    18901 gttggattga atccttttgc ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt
    18961 ttgtcgatgg tcatttctgg cgctttggca gggcttggcg gtgtggttga aggattagga
    19021 acgtatcaaa acttctttgt tcaaacgact tcgctatcta ttggatttga cggaatggct
    19081 gtttccttgc ttggttctgg atcagcaatc ggtattcttt tatcagcgat cttgttcagt
    19141 attttgaaga tcggtggact tggcatgcag acaggagcag gtgttccgtt tgagatcgtc
    19201 aatgtatcga ttgctttgat tatcttcttt gtcgggatca acttcctgat tcgtttcgtc
    19261 atggctaaag tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa
    19321 ccaacgaatc aaaaccatga agggggcgaa ctataatgtc gactatcgaa ttaattgcct
    19381 tgatccttac ttctacactg gtttattcaa caccgctaat tctaacgtca ctaggcggaa
    19441 ctttttctga acgaagcggg atcgtaaacg tagggctaga gggaatcatg gtcatgggtg
    19501 cttttagttc catcgttttc aacttgacga tgagtccatc acttggttct atgacaccat
    19561 ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag
    19621 caacagtcag tttgcgtgct gaccatatta tcagcgggac agtaattaat ctgatggcac
    19681 ctgctttagg tgttttcttg atcaaagcat ggtatggtaa aggtcaaact gataacatca
    19741 cgcagaatct ggggtatttt tctttcccag gattagcaga tattccggtc attggaccaa
    19801 ttttctttaa aaatacattc ttacctgctt attgtgctat tttgattgct gtcctagctt
    19861 gggtgatcat cttcaagacg aaattcggct tgcgcctccg ttcagttggt gaaaatcctc
    19921 aagctgccga cacattaggt atcaatgtat atgctatgcg ttattcaggc gtactgattt
    19981 ctggcttgtt aggcggtatg ggcggtgctg tttttgctca gtcgatttcc ggtaactttt
    20041 cagctggtac gatcgttgga caagggttca tctccatggc tgctatgatc tttggtaagt
    20101 ggaatccttt aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa agcttgagta
    20161 tcattggcgc acaattacca gttatttctt cgattccgcc agtattgctg caaattgctc
    20221 catatttgct gacaatcatt gtgctggttg ttttcttagg gaaagcttct ggtccaaaag
    20281 caaacgggaa gaactatatc aaatcaaaat aatcttttag cgaataaaaa gggtgggtta
    20341 cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc aataaaaata aaaagaaaga
    20401 aggaataatc atgtttaaac gcgtacatct gatcgtcatg gattcagtag gaattggaga
    20461 agcaccagat gccgataagt tcggtgatgt tggttctgat acattaggac acattgcgaa
    20521 agaagcagga ttgaacgttc cacatttaga gcagcttggt ttaggaacga tccgtcctct
    20581 tgaaggtgtg gaatcagtga aagatcatca aggctatgcg acgaaattag aagaagtttc
    20641 tgtagggaaa gatacgatga caggccactg ggaaatcatg ggattgaata tcaaaactcc
    20701 atttcgtgtg tttcctaatg gattccctga tgatctgatc aaacaaatcg aagaattttc
    20761 tggccgtaaa gtcgtatgta acaaacctta tagcggaaca gctgttattg acgattacgg
    20821 tgaacaccaa atgaaaacag gcgatttaat tgtatatact tctgccgatc ctgtccttca
    20881 gatcgcggca catgaagaaa tcattccttt agaagaatta tatcggattt gccaattcgt
    20941 tcgtgatata acaaaagatg atccgtatat gattggccga atcattgcac gtccgtacgt
    21001 gggtgaacca ggagacttca cacgaacaag caatcgccat gattatgcgt tgaatccatt
    21061 cggacacaca gtccttgatt ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa
    21121 gatcaacgat attttcaacg gacaaggaat cactgaatct gtccgcacga aaagcaatat
    21181 ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa tcagctttac
    21241 caatttagta gattttgatg cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca
    21301 tgcgattgaa gaatttgact tacgaattcc agaattgctt gaagcgatgg aagaagacga
    21361 cttgctaatg attacagccg atcacggaaa cgatccgacg tttactggta ccgatcatac
    21421 gcgagaatat gtaccgttat tggtttacag taaaaaaatg aaagaacaag gggcattgcc
    21481 tcaaggatat tattcagata tttcagcaac gattgctgag aactttagtg tttcttcgac
    21541 tgaaaatggc gaaagcttct tgaaattatt aaaataatct ttttaaatag gagaaagcaa
    21601 atgaccaagt taagcgagat gttacaagaa acgacccagt ttatccttga taaaggagta
    21661 aaagaaattg attttggttt gattctaggt tcaggattag gtgaattagc agatgaaatc
    21721 gaagatgctg taatgatttc ttatgatgag atcccgttct ttcctgtttc gacagtagtt
    21781 gggcatgcag gaaagctagt ttatgggtct ctggcaggca agaaagtgtt agccatgcaa
    21841 gggagattcc actactatga aggtcattcc atgcaaacag tgacctatcc agtacgtgtg
    21901 atggccggac taggggccca ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa
    21961 tcatttgtac caggagattt gatgctgatt accgatcata tcaattttac tggtgaaaat
    22021 cctttgatcg gtccgaatga agatgcaatg ggccctagat ttccagatat gagtcaagct
    22081 tatacgcctg actatcaagc aacagcaaaa gcagcagcag aaaaactagg tttggatctt
    22141 aaagaaggcg tatatatggg ctattctgga ccaacttatg aaacgcctgc ggagatccga
    22201 atgtctcgga caataggagc cgatgcagta gggatgtcga ctgttccaga agtcatcgta
    22261 gcagctcaca gcggattaaa agtgttagga atctcttgta ttaccaactt agcagcaggt
    22321 atgcaagcga atctgagcca tgaagaagtt gtagaaacaa ctcagagagt caaacaatct
    22381 tttaaagcat tggttaaaga aacattgaca ttattgtaat aggaggaaca ataaaatgag
    22441 tgtgcatatt gaagcgaaag aaggcgagat cgccgataag attttacttc cgggagatcc
    22501 tttgcgagct aaatatattg cggagacttt tttggaagat ccagtttgtt acaatcaggt
    22561 acgcggtatg ttaggcttta ccggcaaata taaaggagaa cgtatttctg ttcaaggaac
    22621 aggaatggga atgccatcag ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt
    22681 aaagaaattg attcgagtag gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga
    22741 tttagtacta gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc
    22801 ctttcatttt cctccaatca gtgatttcaa tctattactg aaagcatatg agatagctaa
    22861 agaaaagggc tatacggttc atgtaggaaa tgttctttcg gaagattctt tctacaaaga
    22921 tgatttgaca gagacgttcc aattagctga acttggcgtt ttaggagtgg aaatggaagc
    22981 agcggctctt tattacttag gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt
    23041 tagcgaccat ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa
    23101 tgaaatgatc gaagtaggat tggaaacagc gatagcagaa taagaaggaa atgaaaaagc
    23161 tgccctgcat taagtaataa gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg
    23221 gctggactta ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc
    23281 tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga
    23341 atattgtgac ttgtgaccga gaagttggca ggagaaaacc gtttatacga aaaaagggac
    23401 tgtgacaaat cgtgtcacag tccctttttt atagtgtttt ttataggtag tattttttag
    23461 caactgttaa gtcatcattt aattcataaa caagtggttg acctgttgga atttcaagat
    23521 ccatgatgtc ttcgtcagag ataccttcga tatgtttagc tagggcgcgt aatgagttac
    23581 catgtgcagc tactaaaaca gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc
    23641 agaatggcaa agcgcgttct aatgtcactt tcaagttttc tccaccagga atatcgcgtt
    23701 tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta
    23761 atggaggaag agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt
    23821 cagctgtttc ttttttgttc aaaccttgta atttaccata gtgacgttcg tttaagcgcc
    23881 atgatttgat ttgtggtacc caaagttgat ctgaatattc taagatcaag ttacatgttt
    23941 tgattgcacg agttaaaaca gaagtgtaag caacatcaaa ttcgattcca gcttctttga
    24001 ttttgcgtcc gccttctttt gcttcttcga ttccttctgg tgctagattt acatcagccc
    24061 aaccagtaaa ttggttcaat gcattccatt cactaagacc gtgacgagaa aaaactaatt
    24121 ttggcatgtt tagctctcct tttaagatag gtttatattt ctgaaatttt cagttctgtt
    24181 ttattgtaca attttttgga agagatttcc atcgaaaaat tagataagat tctatgagaa
    24241 aacaagtaga gaatataaaa aaacgaacag tgcccacgca actgttcgtt tttaggtatt
    24301 agatgcataa ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa
    24361 ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac
    24421 gtcggatttt tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt
    24481 tacgagcttc atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg
    24541 ctaggttttc cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat
    24601 caagactaat caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc
    24661 gcgtagattt gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt
    24721 cagcgatcac tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct
    24781 catggaaaat ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa
    24841 cgtcatttat ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt
    24901 gattttctaa ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt
    24961 tttcgcaaat tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac
    25021 gacggattgc gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca
    25081 tgtttaggaa aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc
    25141 tttctggctg ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg
    25201 ttaagacttc tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa
    25261 tatgaagtag ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa
    25321 gtttatcgac taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga
    25381 ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga
    25441 tttcatcagc accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg
    25501 gtattcctaa tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac
    25561 gtaaaccttc ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac
    25621 cggtcccgag acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta
    25681 attgttttaa attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca
    25741 ctattttagc gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt
    25801 tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt
    25861 gctttatcta caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata
    25921 aggatgcaga cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa
    25981 ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg
    26041 taaacctcgt aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa
    26101 cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg
    26161 tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg
    26221 tttgtcaaac ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga
    26281 acacagtgtt gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca
    26341 tatcgttcgc ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa
    26401 atacggaact aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct
    26461 actatataga agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt
    26521 aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta
    26581 actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc
    26641 tataatgcct ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa
    26701 caagcaatga aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca
    26761 aactaccaag taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg
    26821 gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa
    26881 atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa
    26941 atggctgatg ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc
    27001 ggcttttata agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga
    27061 ggagaaacaa ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc
    27121 atggcccaca tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt
    27181 aaaatccata aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa
    27241 gaacaagaac gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac
    27301 cgaatcaaca tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca
    27361 ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa
    27421 actgaaacag tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac
    27481 aaaatggaca agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg
    27541 caagcaaacg ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt
    27601 atcgaccttg taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa
    27661 gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt
    27721 gaagctgtag ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc
    27781 actgaagctg agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca
    27841 gtactatgtg gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt
    27901 gattacttgc catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat
    27961 gaagaagtag aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt
    28021 atgactgacc cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa
    28081 tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta
    28141 cagatgcatg ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct
    28201 gctgttggtt tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt
    28261 attcttgaat ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca
    28321 aaagctgacc aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact
    28381 ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt
    28441 cacttagata tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt
    28501 gcgcctcaag tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt
    28561 gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat
    28621 gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa
    28681 tacattccag ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc
    28741 tatccattag tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca
    28801 aatgaaactg ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct
    28861 aatccagtaa tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta
    28921 ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc
    28981 gctggcggcc aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct
    29041 acaactcttc gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac
    29101 gaagatgtac caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa
    29161 taatttctta aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat
    29221 atagtttcgt gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca
    29281 tatacctatc agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca
    29341 aaagaaaaat ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac
    29401 catggtaaaa ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc
    29461 caagctatgg cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca
    29521 atctcaacag ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc
    29581 ccaggacacg cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct
    29641 atcttggtag tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg
    29701 tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat
    29761 gacgaagaat tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa
    29821 ttccctggtg acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac
    29881 gcttcatacg aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact
    29941 ccagaacgtg acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact
    30001 ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa
    30061 gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg
    30121 ttccgtaaat tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt
    30181 gttgcacgtg aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct
    30241 catacaaaat tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact
    30301 ccattcttca ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt
    30361 gttgaattac cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt
    30421 gaattaatcc acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga
    30481 cgtacagttg gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag
    30541 taaagcaaca tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg
    30601 agctttttta tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac
    30661 acgaaataaa agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac
    30721 aagaaaaagc cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt
    30781 agtgaattgc acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac
    30841 acgcccggac gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact
    30901 tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa
    30961 agcgtatgaa caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag
    31021 aactggagct agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat
    31081 catccgtgcg actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa
    31141 acgtcttatc gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga
    31201 cttaccatct ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg
    31261 tgtactcatg accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga
    31321 atctggtgaa ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt
    31381 gaaaacaatg gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga
    31441 agttttatca aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg
    31501 cttcattcgt gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac
    31561 agtagatgtt ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg
    31621 attccaaggg gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg
    31681 ttaccatcgt cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa
    31741 acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg
    31801 tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc
    31861 tttaattact atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta
    31921 aggaatgccg aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact
    31981 taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat
    32041 gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg
    32101 cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg
    32161 ctcaccacaa tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta
    32221 caaacttcct aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc
    32281 tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt
    32341 caaacaagtt cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg
    32401 taacgacttt gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga
    32461 taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct
    32521 tactcaaatt gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg
    32581 atcactgaaa aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact
    32641 cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa
    32701 aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat
    32761 acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta
    32821 ttcgaagctg ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg
    32881 cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg
    32941 ctgaaatcac aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg
    33001 gacgtaacaa caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat
    33061 atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg
    33121 aatatgatcc aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag
    33181 cttacatctt agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag
    33241 cagatatcaa agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc
    33301 acaacattga aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg
    33361 ctcaagtact tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc
    33421 gtatgatctt agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat
    33481 taatcaacat cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg
    33541 gtagcgtaat gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg
    33601 gacgtaaagc accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca
    33661 aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac
    33721 catgtgtcgc acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt
    33781 ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag
    33841 ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg
    33901 cagtttatga tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca
    33961 aattaggtga atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa
    34021 cgaaacgcta atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa
    34081 gcaactgcaa aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc
    34141 agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct
    34201 ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta
    34261 gatgttgaaa acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt
    34321 ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg
    34381 gtagtcgtat cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg
    34441 aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc
    34501 tgagttctta cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc
    34561 tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac
    34621 agctaaacca ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga
    34681 attaaacaaa ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga
    34741 tctagatgca aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt
    34801 ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa
    34861 aactcaagta tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga
    34921 aggaactgtt cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga
    34981 tacaacatac ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac
    35041 taaaaaaaac actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac
    35101 cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc
    35161 ggtgaatacg gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct
    35221 gctcgtatcg caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc
    35281 cctcacaaat cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca
    35341 cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt
    35401 cctgaagaag tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact
    35461 aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga
    35521 attaaccact gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct
    35581 tagattccaa ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa
    35641 atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc
    35701 cattaagcgt atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc
    35761 agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta
    35821 tggcaaacgc atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa
    35881 agttggcgac atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg
    35941 tttgttggaa gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag
    36001 attgaatgca aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa
    36061 agtcgctgat aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg
    36121 tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg
    36181 tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc
    36241 tcgtcgtaca gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga
    36301 tgataaaagc cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa
    36361 caacttcatg aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca
    36421 aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg
    36481 aaaagacaaa aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat
    36541 tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg
    36601 tggaattgtt gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac
    36661 tggtgtagct ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa
    36721 aaaaaccggt gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa
    36781 ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta
    36841 tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga
    36901 tgcggtatca aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg
    36961 tcaaaaacca atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg
    37021 aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa
    37081 attggtttct gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt
    37141 tgacggtcgt ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga
    37201 ttatgattta gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc
    37261 tgatgaagaa tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa
    37321 ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt
    37381 ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa
    37441 gttccatctt tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt
    37501 gaagaaagct agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc
    37561 atgacagatc caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat
    37621 gagtctttag aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt
    37681 gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt
    37741 ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa
    37801 ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt
    37861 atcgcaatca tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat
    37921 atcggcggcg aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct
    37981 ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca
    38041 agacggaaac aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc
    38101 agatatcaaa atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa
    38161 agaaatgaaa acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt
    38221 cagcgaaggt ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca
    38281 aggatcaaaa cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc
    38341 tggagtaact gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga
    38401 agaagttggc gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg
    38461 taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata
    38521 atcgtttaga aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta
    38581 ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact
    38641 gcttgaaata gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata
    38701 agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg
    38761 agcgcccacg cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg
    38821 acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa
    38881 caaaaacaga agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag
    38941 gcattaaagt agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt
    39001 tagctgaagc tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat
    39061 ggtttatatc gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt
    39121 aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga
    39181 caaaaacgga cacgtagggt ttggtacagg taaagctcaa gaagttcctg aagcaatccg
    39241 taaagcaatt gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat
    39301 cccacacgaa gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga
    39361 aggttctggg gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc
    39421 agatatcact tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt
    39481 tgaaggtttg aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt
    39541 tgaagaatta atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa
    39601 cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca
    39661 aagattaaca gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact
    39721 gtttctcacc tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta
    39781 aggaggtgcc aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt
    39841 acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca
    39901 aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac
    39961 accattgttc cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc
    40021 agtcattaat cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc
    40081 tttagttgaa gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg
    40141 ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc
    40201 aattgaagcg gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct
    40261 tttaaagtaa aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt
    40321 cgcctaggtg cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct
    40381 tctttgccat ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc
    40441 attttctcaa tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa
    40501 atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa
    40561 ctgaatcaag cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc
    40621 actgctggat tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact
    40681 tacgtaacga tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa
    40741 cagatcacag aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt
    40801 tctagattgc ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct
    40861 tcagacatat ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc
    40921 gtgacattcg taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa
    40981 cgcgtagctg gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt
    41041 attccagtta tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg
    41101 agcggtagat tcggttctga aaactggtac aaagtgatga tggagatctt taactataat
    41161 acagcacctg gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc
    41221 tttgttcaag taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata
    41281 ccaagcgtac gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta
    41341 agtacagttg gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg
    41401 gtttgggatc ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt
    41461 gtagctttag aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga
    41521 ttcattgagt aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt
    41581 aggagggaaa acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca
    41641 agctgaaaaa atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg
    41701 cgcagcgatg caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg
    41761 tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga
    41821 tacagagaaa ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct
    41881 ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg
    41941 tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac
    42001 ttaccataaa attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca
    42061 tgaattctat caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa
    42121 catcaaaaat agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat
    42181 tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata
    42241 gcacgattgc ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta
    42301 tgatagaata tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt
    42361 tgaggtggga actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg
    42421 aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt
    42481 tgccgaatgc aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt
    42541 caggtaaaat tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat
    42601 tgtctccata cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg
    42661 taaaactaca ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca
    42721 ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca
    42781 taaacaacgt caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat
    42841 attcctcgtg ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact
    42901 actgctaaaa agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta
    42961 acgaatgaac aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat
    43021 cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga
    43081 atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact
    43141 cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt
    43201 taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa
    43261 gcaggtattg cacatatcca ttctacattc aataacacta tcatcatgat cactgatact
    43321 cacggaaatg cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa
    43381 tcaactcctt ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac
    43441 ggattaaaga ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt
    43501 cgttcattac aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct
    43561 cataatggtg cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt
    43621 tttatcagta acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat
    43681 gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt
    43741 catcgttgaa cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat
    43801 tttattatct tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca
    43861 tgaattctct accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa
    43921 aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg
    43981 acctgctaca gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa
    44041 agatatgtac atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa
    44101 acctggacgt ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt
    44161 tcttccagtc gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac
    44221 acgtgttgga caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc
    44281 aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat
    44341 ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac
    44401 acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta
    44461 caattgtttg aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc
    44521 tgaaatgatc aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt
    44581 gcacgactta ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac
    44641 caacgtgagt tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga
    44701 tttaacaact gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga
    44761 agttcgctca actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg
    44821 tcgtcaagct gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga
    44881 catcgtcatt gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga
    44941 acgtcaaggc ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc
    45001 aatggttgtc ttagaacttg tctaaaatca cactaataac atcactttat agatgattct
    45061 tttagagtgt tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa
    45121 gcgaatatcg cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac
    45181 ctgtcatgac agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa
    45241 attttgtctt tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg
    45301 agccaattat tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg
    45361 cattgaaaga tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca
    45421 atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag
    45481 ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga
    45541 tggttggaat ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg
    45601 acgttgcttt tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga
    45661 aagatgcact tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat
    45721 caggagggca aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca
    45781 ttattttaga tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta
    45841 cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg
    45901 atgaagcagc taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag
    45961 gtacaccaaa agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac
    46021 catttcctga aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat
    46081 atatgacaga agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt
    46141 cacttatcag ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat
    46201 caaagaaaac agttatactg cattagtagg gcataccgga agcgggaaat caaccttact
    46261 tcagcatttg aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga
    46321 tattcagccg gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt
    46381 ctttcagttt ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg
    46441 tcctaaaaac tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga
    46501 acaagttggg ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca
    46561 aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga
    46621 tgaaccaacg gcaggacttg atccccaggg tagaaaagaa atgatggaga tgttttggcg
    46681 tcttcataag gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc
    46741 caattttgca gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc
    46801 gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc
    46861 caccgagttt gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac
    46921 agctgaagaa ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata
    46981 aattgatatt tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag
    47041 ctaaattatt agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa
    47101 cgtacctttt gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga
    47161 aattttttat ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc
    47221 aggtcttgtt tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg
    47281 aatttggtgt gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt
    47341 cgactctact cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc
    47401 ttcgtccatt gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg
    47461 ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg
    47521 ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt
    47581 tgctgattcc tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg
    47641 aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata
    47701 atagagacac gatggttatc attgcttttg tattattaac tgtgctactg atctttttga
    47761 gaggataaac gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa
    47821 ggatgcgagc agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc
    47881 tctttctttt atctgaaaag aaagaacgaa aaaattttat aaaaaaatca ttattccaga
    47941 taacctttta ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcatac
    48001 gacggaacaa attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa
    48061 gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga
    48121 aggacagatg ccggagtaca tgcgattggg caagtaatcc attttgatta tccagaggag
    48181 cgtccgttag aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta
    48241 agacaagtag aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg
    48301 tatcaatttc gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt
    48361 tattttcctt accctcttga tttggaaaaa attcagcggg cgcttcctga tttattaggg
    48421 actcacgatt tcacctcttt ttgtgcatca ggaagctcga tagaggataa agtacgtacg
    48481 atctacgagg ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg
    48541 ggaaatggct tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc
    48601 aatggccgat tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca
    48661 gcaggaccta cagcacatcc tgaaggactt tatttgtatg aagtgaaata tgaataagag
    48721 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttacttctca
    48781 aaattttgca aaatttcggc ttattttcga aggagctact tctggaacac cgtgaatcag
    48841 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca accaaacaaa aatagctcgt
    48901 catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
    48961 gaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt
    49021 tcctcatatt ttgccgtttg ttgagctaat accgaaggtc agctgattga acaccgttta
    49081 agaaagaggt cgtgagcctg tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca
    49141 gcttctcatc tttgaggaat atcgtgactt gtggacgagg cgttgataag tgaatcatct
    49201 ttttgttacc tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgccctta
    49261 atgaggacag tcggaacagg tttgtcattt tta
//
LOCUS       DAXWLH010000003        36252 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXWLH010000003 DAXWLH010000000
VERSION     DAXWLH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.003"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(82..777)
                     /locus_tag="ACGE36_000113"
     CDS             complement(82..777)
                     /locus_tag="ACGE36_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFF8382547.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE36_000114"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE36_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFF8382548.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="ACGE36_000115"
     CDS             complement(1976..2167)
                     /locus_tag="ACGE36_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFF8382549.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="ACGE36_000116"
     CDS             complement(2188..3078)
                     /locus_tag="ACGE36_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFF8382550.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="ACGE36_000117"
     CDS             complement(3065..3832)
                     /locus_tag="ACGE36_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8382551.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE36_000118"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE36_000118"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFF8382552.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE36_000119"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE36_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFF8382553.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE36_000120"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE36_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFF8382554.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLNTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8403..8786
                     /locus_tag="ACGE36_000121"
     CDS             8403..8786
                     /locus_tag="ACGE36_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382555.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE36_000122"
     CDS             complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE36_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFF8382556.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="ACGE36_000123"
     CDS             complement(9730..10548)
                     /locus_tag="ACGE36_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFF8382557.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE36_000124"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE36_000124"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8382558.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE36_000125"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE36_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFF8382559.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE36_000126"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE36_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFF8382560.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="ACGE36_000127"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="ACGE36_000127"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFF8382561.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14567..14809
                     /gene="yaaA"
                     /locus_tag="ACGE36_000128"
     CDS             14567..14809
                     /gene="yaaA"
                     /locus_tag="ACGE36_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFF8382562.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14796..15920
                     /gene="recF"
                     /locus_tag="ACGE36_000129"
     CDS             14796..15920
                     /gene="recF"
                     /locus_tag="ACGE36_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFF8382563.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15917..17863
                     /gene="gyrB"
                     /locus_tag="ACGE36_000130"
     CDS             15917..17863
                     /gene="gyrB"
                     /locus_tag="ACGE36_000130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFF8382564.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17883..20354
                     /gene="gyrA"
                     /locus_tag="ACGE36_000131"
     CDS             17883..20354
                     /gene="gyrA"
                     /locus_tag="ACGE36_000131"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFF8382565.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20590..20889
                     /gene="rpsF"
                     /locus_tag="ACGE36_000132"
     CDS             20590..20889
                     /gene="rpsF"
                     /locus_tag="ACGE36_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFF8382566.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20939..21451
                     /gene="ssb"
                     /locus_tag="ACGE36_000133"
     CDS             20939..21451
                     /gene="ssb"
                     /locus_tag="ACGE36_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8382567.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21477..21713
                     /gene="rpsR"
                     /locus_tag="ACGE36_000134"
     CDS             21477..21713
                     /gene="rpsR"
                     /locus_tag="ACGE36_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFF8382568.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21856..23829
                     /locus_tag="ACGE36_000135"
     CDS             21856..23829
                     /locus_tag="ACGE36_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFF8382569.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23835..24287
                     /gene="rplI"
                     /locus_tag="ACGE36_000136"
     CDS             23835..24287
                     /gene="rplI"
                     /locus_tag="ACGE36_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFF8382570.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24561..25928
                     /gene="dnaB"
                     /locus_tag="ACGE36_000137"
     CDS             24561..25928
                     /gene="dnaB"
                     /locus_tag="ACGE36_000137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFF8382571.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26708..28000
                     /locus_tag="ACGE36_000138"
     CDS             26708..28000
                     /locus_tag="ACGE36_000138"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFF8382572.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            28787..29650
                     /locus_tag="ACGE36_000139"
     CDS             28787..29650
                     /locus_tag="ACGE36_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8382573.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(29779..31401)
                     /locus_tag="ACGE36_000140"
     CDS             complement(29779..31401)
                     /locus_tag="ACGE36_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8382574.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            31650..32171
                     /locus_tag="ACGE36_000141"
     CDS             31650..32171
                     /locus_tag="ACGE36_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8382575.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(32357..34087)
                     /gene="spxB"
                     /locus_tag="ACGE36_000142"
     CDS             complement(32357..34087)
                     /gene="spxB"
                     /locus_tag="ACGE36_000142"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFF8382576.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            34252..35070
                     /locus_tag="ACGE36_000143"
     CDS             34252..35070
                     /locus_tag="ACGE36_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFF8382577.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(35173..35370)
                     /locus_tag="ACGE36_000144"
     CDS             complement(35173..35370)
                     /locus_tag="ACGE36_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382578.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            35539..36117
                     /locus_tag="ACGE36_000145"
     CDS             35539..36117
                     /locus_tag="ACGE36_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8382579.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        10991 a         7000 c         7198 g        11063 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtatt taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14461 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14521 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14581 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14641 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14701 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14761 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14821 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14881 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    14941 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15001 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15061 aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg
    15121 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15181 agaccaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15241 atcttgtcca ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg
    15301 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15361 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15421 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15481 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15541 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15601 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15661 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg
    15721 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc
    15781 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15841 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15901 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    15961 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16021 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16081 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16141 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16201 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16261 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16321 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16381 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16441 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16501 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16561 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16621 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16681 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16741 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16801 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16861 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16921 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    16981 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17041 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17101 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17161 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17221 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17281 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17341 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17401 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17461 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17521 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17581 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17641 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17701 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17761 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17821 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17881 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    17941 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18001 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18061 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18121 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18181 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18241 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18301 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18361 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18421 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18481 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18541 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18601 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18661 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18721 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18781 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18841 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18901 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    18961 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19021 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19081 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19141 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19201 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19261 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19321 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19381 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19441 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19501 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19561 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19621 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19681 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19741 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19801 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19861 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19921 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    19981 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20041 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20101 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20161 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20221 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20281 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20341 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20401 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20461 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20521 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20581 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20641 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20701 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20761 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20821 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20881 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    20941 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21001 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21061 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21121 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21181 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21241 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21301 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21361 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21421 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21481 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21541 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21601 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21661 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21721 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21781 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21841 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21901 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    21961 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22021 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22081 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22141 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22201 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22261 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22321 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22381 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22441 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22501 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22561 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22621 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22681 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22741 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22801 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22861 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22921 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    22981 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23041 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23101 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23161 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23221 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23281 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23341 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23401 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23461 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23521 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23581 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23641 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23701 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23761 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23821 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23881 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    23941 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24001 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24061 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24121 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24181 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24241 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24301 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24361 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24421 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24481 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24541 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24601 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24661 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24721 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24781 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24841 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24901 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    24961 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25021 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25081 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25141 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25201 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25261 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25321 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25381 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25441 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25501 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25561 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25621 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25681 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25741 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25801 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25861 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25921 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    25981 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26041 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26101 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    26161 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    26221 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    26281 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    26341 atctgcggta atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt
    26401 cttattactt gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta
    26461 gcttcttcga aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc
    26521 cttcttattt cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa
    26581 aaagataaag ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt
    26641 atttgaaata tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg
    26701 tgttcacatg tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa
    26761 aatcacagac tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa
    26821 tgcagggcac acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg
    26881 gattttttat aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc
    26941 attggttaaa gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat
    27001 ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga
    27061 tgctaagggc gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga
    27121 taaagcagct cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga
    27181 acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga
    27241 accacttgat tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca
    27301 atacgtcaca gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt
    27361 gtttgaaggc gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac
    27421 ttcttctaac ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat
    27481 caataaagtt gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc
    27541 aactgaatta tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac
    27601 gacaactggt cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa
    27661 acgagtttca ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga
    27721 aacagtaaaa atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc
    27781 aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga
    27841 agacattaca ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg
    27901 tcgtatctca gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac
    27961 acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa
    28021 tcaggacgtt cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa
    28081 gaggtagtga aagagacgtt tagccttatt gccgataggc tgtctcttga acactgttta
    28141 ttcagaataa gggactgtga caaaaaccgt gtcacagtcc ctttttccga acaaacggtg
    28201 ttcaaaagct tgcatctacg gcactaagcc caatcagatg aaaaatatgg agtgctattt
    28261 ttatctgatt gttcttactg cttgatgcag agcagctttt tcacaacctc tccactaaac
    28321 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg
    28381 ttttttgaaa ttgttccttg tgactcggag ctaaacggca ggctcacaac ctcttggtaa
    28441 acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact
    28501 attttttccg tttttttctc taatactcag gtcaaaacgc tgatttcaca acctctgatt
    28561 cacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
    28621 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctctta
    28681 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
    28741 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
    28801 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
    28861 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
    28921 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
    28981 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
    29041 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
    29101 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
    29161 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
    29221 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
    29281 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
    29341 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
    29401 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
    29461 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
    29521 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
    29581 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
    29641 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
    29701 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
    29761 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
    29821 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
    29881 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
    29941 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
    30001 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
    30061 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
    30121 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
    30181 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
    30241 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
    30301 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
    30361 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
    30421 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
    30481 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
    30541 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
    30601 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
    30661 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
    30721 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
    30781 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
    30841 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
    30901 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
    30961 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
    31021 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
    31081 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
    31141 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
    31201 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
    31261 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
    31321 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
    31381 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
    31441 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
    31501 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
    31561 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
    31621 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
    31681 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
    31741 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
    31801 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
    31861 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
    31921 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
    31981 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
    32041 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
    32101 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
    32161 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
    32221 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
    32281 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
    32341 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
    32401 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
    32461 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
    32521 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
    32581 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
    32641 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
    32701 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
    32761 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
    32821 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
    32881 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
    32941 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
    33001 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
    33061 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
    33121 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
    33181 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
    33241 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
    33301 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
    33361 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
    33421 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
    33481 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
    33541 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
    33601 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
    33661 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
    33721 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
    33781 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
    33841 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
    33901 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
    33961 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
    34021 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
    34081 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
    34141 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
    34201 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
    34261 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
    34321 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
    34381 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
    34441 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
    34501 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
    34561 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
    34621 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
    34681 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
    34741 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
    34801 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
    34861 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
    34921 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
    34981 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
    35041 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
    35101 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
    35161 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
    35221 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
    35281 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
    35341 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
    35401 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
    35461 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
    35521 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
    35581 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
    35641 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
    35701 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
    35761 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
    35821 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
    35881 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
    35941 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
    36001 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
    36061 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
    36121 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
    36181 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
    36241 gattactagt tt
//
LOCUS       DAXWLH010000004        35796 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXWLH010000004 DAXWLH010000000
VERSION     DAXWLH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.004"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..884
                     /locus_tag="ACGE36_000146"
     CDS             <1..884
                     /locus_tag="ACGE36_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFF8382580.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACGE36_000147"
     CDS             932..3061
                     /locus_tag="ACGE36_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFF8382581.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACGE36_000148"
     CDS             3036..3725
                     /locus_tag="ACGE36_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382582.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACGE36_000149"
     CDS             complement(4133..4819)
                     /locus_tag="ACGE36_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8382583.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACGE36_000150"
     CDS             complement(4955..5149)
                     /locus_tag="ACGE36_000150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382584.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACGE36_000151"
     CDS             complement(5199..5639)
                     /locus_tag="ACGE36_000151"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8382585.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACGE36_000152"
     CDS             complement(5643..6488)
                     /locus_tag="ACGE36_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFF8382586.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACGE36_000153"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACGE36_000153"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFF8382587.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8131..9333)
                     /locus_tag="ACGE36_000154"
     CDS             complement(8131..9333)
                     /locus_tag="ACGE36_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382588.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(9320..11107)
                     /locus_tag="ACGE36_000155"
     CDS             complement(9320..11107)
                     /locus_tag="ACGE36_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFF8382589.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(11120..12073)
                     /locus_tag="ACGE36_000156"
     CDS             complement(11120..12073)
                     /locus_tag="ACGE36_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8382590.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(12258..12608)
                     /locus_tag="ACGE36_000157"
     CDS             complement(12258..12608)
                     /locus_tag="ACGE36_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8382591.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            12808..13887
                     /gene="pepA"
                     /locus_tag="ACGE36_000158"
     CDS             12808..13887
                     /gene="pepA"
                     /locus_tag="ACGE36_000158"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFF8382592.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14030..14350
                     /locus_tag="ACGE36_000159"
     CDS             14030..14350
                     /locus_tag="ACGE36_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8382593.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            14372..14836
                     /locus_tag="ACGE36_000160"
     CDS             14372..14836
                     /locus_tag="ACGE36_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8382594.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            15032..15637
                     /gene="ytpR"
                     /locus_tag="ACGE36_000161"
     CDS             15032..15637
                     /gene="ytpR"
                     /locus_tag="ACGE36_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFF8382595.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(15676..16965)
                     /locus_tag="ACGE36_000162"
     CDS             complement(15676..16965)
                     /locus_tag="ACGE36_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFF8382596.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            17393..17872
                     /gene="rlmH"
                     /locus_tag="ACGE36_000163"
     CDS             17393..17872
                     /gene="rlmH"
                     /locus_tag="ACGE36_000163"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFF8382597.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            18225..18539
                     /locus_tag="ACGE36_000164"
     CDS             18225..18539
                     /locus_tag="ACGE36_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8382598.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            18555..18941
                     /locus_tag="ACGE36_000165"
     CDS             18555..18941
                     /locus_tag="ACGE36_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8382599.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            18970..20355
                     /locus_tag="ACGE36_000166"
     CDS             18970..20355
                     /locus_tag="ACGE36_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8382600.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            20358..20511
                     /locus_tag="ACGE36_000167"
                     /pseudo
     CDS             20358..20511
                     /locus_tag="ACGE36_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            20534..21739
                     /gene="mobT"
                     /locus_tag="ACGE36_000168"
     CDS             20534..21739
                     /gene="mobT"
                     /locus_tag="ACGE36_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFF8382601.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            22523..24433
                     /gene="ltrA"
                     /locus_tag="ACGE36_000169"
     CDS             22523..24433
                     /gene="ltrA"
                     /locus_tag="ACGE36_000169"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8382602.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKRNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            24537..24761
                     /locus_tag="ACGE36_000170"
     CDS             24537..24761
                     /locus_tag="ACGE36_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382603.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            24878..25375
                     /locus_tag="ACGE36_000171"
     CDS             24878..25375
                     /locus_tag="ACGE36_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFF8382604.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            25462..25854
                     /locus_tag="ACGE36_000172"
     CDS             25462..25854
                     /locus_tag="ACGE36_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8382605.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            25838..28285
                     /locus_tag="ACGE36_000173"
     CDS             25838..28285
                     /locus_tag="ACGE36_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8382606.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            28288..30471
                     /locus_tag="ACGE36_000174"
     CDS             28288..30471
                     /locus_tag="ACGE36_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8382607.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            30468..31469
                     /locus_tag="ACGE36_000175"
     CDS             30468..31469
                     /locus_tag="ACGE36_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8382608.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            31484..32398
                     /locus_tag="ACGE36_000176"
     CDS             31484..32398
                     /locus_tag="ACGE36_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8382609.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            32712..32759
                     /locus_tag="ACGE36_000177"
                     /pseudo
     CDS             32712..32759
                     /locus_tag="ACGE36_000177"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32775..34694
                     /gene="tet(M)"
                     /locus_tag="ACGE36_000178"
     CDS             32775..34694
                     /gene="tet(M)"
                     /locus_tag="ACGE36_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8382610.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            34792..>35796
                     /gene="tet"
                     /locus_tag="ACGE36_000179"
     CDS             34792..>35796
                     /gene="tet"
                     /locus_tag="ACGE36_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8382611.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     S"
BASE COUNT        11676 a         6492 c         7334 g        10294 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataacgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gatgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctat gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtatttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattgttt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat
     8101 gtttaaaaat gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt
     8161 ctcaggaggg atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt
     8221 acgatacgta aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata
     8281 cggcgttaca gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg
     8341 tttacgataa gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac
     8401 ttttggatac atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt
     8461 gtcgtttcct tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt
     8521 caatatgatc gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca
     8581 aaagataccg gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat
     8641 taaatactta tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg
     8701 tgttgtcaac aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa
     8761 ttgttggttg gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt
     8821 caaaatccaa atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc
     8881 aagcgaaatc aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct
     8941 cgtcaatcca atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact
     9001 catccctagc agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc
     9061 cgccatagac atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat
     9121 catcaaatga tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga
     9181 aggatacatt ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga
     9241 gtgctgcgga atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac
     9301 aaaagcaaaa aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt
     9361 tttctctttt atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa
     9421 caaacacata ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa
     9481 gcataccggg aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg
     9541 cactgccgat agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga
     9601 tttttttcat tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcataccat
     9661 aattattcgt gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc
     9721 agaacccgaa cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat
     9781 ttcttgccca tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat
     9841 ttttatgcca cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg
     9901 ggagtagctg attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag
     9961 ttctagaaat ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat
    10021 aacgagccat caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt
    10081 gaaagaatgc ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc
    10141 cggttactac ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta
    10201 cgtaaatctg attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta
    10261 aggaaaataa aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga
    10321 ttggaaaaac cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga
    10381 tgtttctttt taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt
    10441 gaaaaatcga aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa
    10501 atagcttgac cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca
    10561 tagattgggc gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca
    10621 gataaggaag ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag
    10681 agaaagcctg tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa
    10741 tcgattgtaa aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa
    10801 tacttccatc agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct
    10861 gtctgccaat agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg
    10921 caactttgtt ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa
    10981 ccatggctaa accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa
    11041 aagcaaaacc tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct
    11101 tatgcataaa aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt
    11161 ataaattcaa ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg
    11221 ttactaatga aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc
    11281 gtgtaataaa gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa
    11341 ataaaaagaa gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca
    11401 ttttgatgat catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc
    11461 taccttcagg acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta
    11521 aagcatgaat cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt
    11581 tgacaaaaat tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat
    11641 ataagtaagt aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat
    11701 tttcatcaaa ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag
    11761 aacgtaattt ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta
    11821 ccatcaaata ataatcggca tcaatatcaa aaaacatttg acggattaca ttcccttttc
    11881 cttgacgtgg ttctttcttt acgattgctc cgccttgaac ggccaattcg tatgttcgat
    11941 cggtcgaatt attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt
    12001 caataacagt tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt
    12061 cacgtgattc catttgtcac tctccttata taataaaact aatcttattt caataatacc
    12121 gtataataac tacaaatact agctattcca agaggaaata gccctatttt tataaaaatc
    12181 acccactata aaagaaggat aggatacctc tcttttagat agtgggtgcg tattcgttat
    12241 ttcatttttt tgacgtatta atctattgcg cccttttttc ttgaaatgtc taatacggtt
    12301 cctgtatgag catctgctaa aaattcataa taaacagttt cgccatcttc tatccgggaa
    12361 agcccacccc gataagcact cgaccgaacg gcgaatttcc gaataggttc tttttcaaaa
    12421 gagatccaag caccttcgat tgcgccttct tttaaaaaag atgttttgat attttcaagc
    12481 acttggtctg ctgataacac ttttttgcga tgataccaaa tcgttgaagc taccccactc
    12541 aataatccta gaccgatccc aacggctaac ccgcctttaa aatagcttaa ttcgctttct
    12601 tcatacatgt ggatatacct ccttcttctc acttgctact ctttgtgttt attttaccac
    12661 actctcatat tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata
    12721 cgatcggtct gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt
    12781 ttagtctgaa atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag
    12841 ttaacagaat tacaaggaac aagcggtttt gaacaagacg ttcgcgccta tatggaaagt
    12901 catatggaac cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttgggctg
    12961 cgccaccatg aagaagcgga tgcacctcgt gtaatggtcg cagcacatat ggatgaagtc
    13021 ggattcatgc tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcgga
    13081 tggaaccctt acgttgtttc tgctcaacgt tttacactta aaacctctac tggtaagaat
    13141 tatccatgta tttcttcttc cgttccccca catttattac gaggaacaag cggacaaaaa
    13201 tcagtagaag taacagatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg
    13261 agttacggtg ttcttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc
    13321 aacgggaaga atatcatcag caaatcgtgg gacaaccgct atggctgtac aatggtatta
    13381 gaagctttag aagcgttaca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat
    13441 gtccaagaag aagtaggcct gcgtggttct aaagcttctg tcaataaatt caagccagat
    13501 ctgttctttg cagtagactg ctctgcagca gatgacacag tgacgaaaaa tggcacattc
    13561 ggacatttag gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct
    13621 cgtttgcgtg agtacttgct agatatcgct gaaacaaaca acatccctta tcaatacttt
    13681 gtttcaaaag gcggtacaga tgcaggtgct gctcatacac aaaatgaagg aatcccaagt
    13741 acagtgattg gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc
    13801 gattttgaag ctgcacgaga aatgttgatt caaacattga aaggtttaga taaatcaaca
    13861 gtgaacacga tcgtatacgg gaaataacgc gatagtgaaa ctataaaatc tggcaaaagc
    13921 aaccttttgt cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag
    13981 gtgtataatt taattgaaaa atacataaac agaaagtaga ggaaaaagaa tgattatccc
    14041 aaatagttta gaagaattag caggctacgt agaaactgga aaaagtgtat ttttctttac
    14101 tgccggctgg tgcggagact gccgttttat caaaccgcaa atgcctgaaa tcgaaaatga
    14161 cttttctgat tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga
    14221 atggaacatt tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg
    14281 tctagtgaat aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaat
    14341 aaaaggctga catctgttag gaggggtcaa gatgaatcaa gaatataaga acattttagt
    14401 aggtattgac ggtagtgaac aagcacttgc tgctttcaaa aaagcgttag aagtggctcg
    14461 ccgtaataat gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataattttat
    14521 gggctatgct cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaagcaaat
    14581 gatcgaagaa tgtaaagaat atggaaaatc tgttgattac acaaatatag aaggaattat
    14641 tgcatacggc tctgttcgtg aatctatggc agtcaaattg ccaaaaaaat atgcaatcga
    14701 tctaatcatg gttgggcagt ctggactaaa tgcagtcgaa cgctttatga caggaagtgt
    14761 agccagctac gtcatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga
    14821 acagccgagt aattaagcaa gtggaacttg tataaccttt ttaatcaaac agtaaaggga
    14881 ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa atggtgttcc agaagaagct
    14941 tcttcggaaa taaagcatga aaaaaatctc atgcagtgaa tgcaattttt tgttaagata
    15001 aatctcagca aaggataatg gaggaaagaa catgattttt gcttataaca aagcacacgt
    15061 cggagacact ttgatggtta tcatagccga cgataaagga acagaaaaca ctgtcgaacg
    15121 taaatggaat gttgctcgga tcaaagatga acaaggtcaa gttgttgctt ggaacttttt
    15181 ccatatctcc gatcatctaa caattgaagg gaacggccaa gtaagcttaa cagatgaaga
    15241 attagcgaaa ttgaatcgat tgattaaaga agcaggattc gaagaacagc ttgaagctga
    15301 tcatgaaccg aagatcgttg tcgggtatgt aaaaacatgc gtcccgcatc ctgactctga
    15361 ccatttatcg attactgaga cagaagtgga caatgggaca gtattgcaaa ttgtttgcgg
    15421 tgcaccaaat atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag ccatgatgcc
    15481 agatggaatg atgatctggc caggaacttt gcgaggggta gccagctatg gaatgatttg
    15541 ttctgctcgt gaattgcatt tgccaaatgc tccagaaaaa aaaggaatct tagtattgcc
    15601 tgaggatgcc gtagtcggag aagtatttcc aaaataaaga ggttgtgaca agtctttgtc
    15661 acaacctctt ttttattaat tttcactatt ttgattttgt tttaatgccg attgatcgat
    15721 cgtcaattca acagtagttg ttttttcttt cgagccttca tagtaagtga ttttgacaga
    15781 atctcccact tttttcttat acaaagcaga ttgaagttct acaccagaag aaacttcttt
    15841 gtcatcgatc ttagtaatga cgtcgtattg tttcaaccca gctttttctg ccggtgtagc
    15901 tgtttggaca tttgtcacga tgacaccgtt agttacagat gaaggaatct tcaagatttg
    15961 ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt gtgataccta gagctggacg
    16021 tgtcactttt ccgtcttttt ccagttggtt gatgatattc actacatcgt tacttgggat
    16081 ggcaaatccc attccttcta cgcttacatt agaagtcgat tcagaagtac ttgcgatctt
    16141 acttgagttg ataccgataa cttgtccttc gatattcact aaaggaccac cagaatttcc
    16201 cgggttgatc gctgcatctg tttggatcgc attgatattg actgtttcgt tcgattcatt
    16261 cgtacttgtt acttgtcggt tcaaggaaga aatgattcct gaagtgacag aattcgcata
    16321 ttcagatcct aatggggaac caatcgcaat cgcaggttca ccaaccttca aagcacttga
    16381 gtccccaaaa gaggcaaccg tttcgacttt gtctgaattg atcttcaaca ccgctaaatc
    16441 tgaataggca tctgttccaa ctaattcagc ttttaccttc gtaccgtctt tcatcatgac
    16501 ttccaaacct tgctgtccat ctactacgtg attgtttgtg actacataag cagtattccc
    16561 gtcttttttg tagattacgc cgcttccttc actataagct tccaaattac tatcatctga
    16621 gctgctactt tcttcttgct gcccgaataa tccgccaaaa ccgcttgatt ggttttgact
    16681 ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta tctaccgctt tggtgatatc
    16741 agaatctaca ttgactttga cattttccac aactgtttca ccggcagaat tttgattgcc
    16801 gcttgtagca gtagaactat tgttgccagt gcccatcgct gcataaaaaa tgccagctgt
    16861 cagtagtcct ccaacgatcc ctccaacaag ccccagaccc aacttacgcc agaggctatt
    16921 atttttattt tttttcatct ttggtgtcac attttttcta tccatgtgtt cctcctccaa
    16981 ttacattcat ttatctactg ttagtatagt cttatttgtt gactttagtc tactttgaga
    17041 ctttgaactt tttatgaaaa actttaggaa aaatcattgt tttcagatga aaatatttga
    17101 ttttcttttt ccacagatat tgtgtaaaac tacgcggata ggattggaaa aataagggat
    17161 aaaacatatc cacagcctgt gtataaagtg gataaattct tataaaacgt atgtttgcct
    17221 aatgaatgtg atgaaataag gtaaaacaac gctgttttct gtggataaac ctgtggatgt
    17281 tgtgcataac tgttggggaa atctttttaa caaaaatagt caaaataaac atatagacga
    17341 aaaaattgat tataataaac aaagtgagcg tgaagatcaa aggagagaaa acatgaatat
    17401 caaaattatt tcagtaggaa aattaaaaga aaaatacttg atccaaggta tcaatgagta
    17461 tgtcaaacgg ctgaatgcct atgcgaaaat cgaattgatc gaagttcctg acgaaaaagc
    17521 cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag gagaacggat
    17581 cttagcgaaa atcaaagaac aagaatacgt ctatggctta gcaattgaag ggaaaaatcc
    17641 cacaagcgaa tcttttgcta aacaaatcga ccaattaggt atccaaggaa aaagccacct
    17701 tgtttttgtt atcggcggtt ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc
    17761 tcagatctct tttggcaaaa tgacgtatcc acatcaattg atgcgcctga tcctcgtcga
    17821 gcaaatctac cgtgccttcc ggatcaacgc gggggctcca taccacaaat aatgatattt
    17881 tttaagagaa aaatagcata aaaatctagt tatccgcata aaaactggac ttatcacact
    17941 ttatcaaggt caaaaccact caatttacta ctaatttact acttatgaat gagctttgat
    18001 acgacgattt atccttgaaa agtgaagata taaagatact tccaataaaa tttgaatatt
    18061 taataggtag acacttcaaa aaatgaggtg tctatttttt tacccgattt tgaaaggaag
    18121 tgaacttatg aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct ttaagaccat
    18181 caaacattca ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg
    18241 tgattcccaa catggaaaaa acattcggca atttagaatt tgctggcgag gataaagtcg
    18301 ttcagcgaag aatcaacgga cggctaactg tcttatcaag aagctataat ctctattctg
    18361 atgttcaaag agcagatgat attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt
    18421 tcggctttga ggaacgtgtg aagttagtca atccacgtat taccgcagag ggctacaaaa
    18481 tcggcactcg tggttttaca aattaccttt tacatgctga cgacatgata aaagaataaa
    18541 gaaagagagg aaaaatgatg agattagcaa atggcattgt attagataaa gacacgactt
    18601 ttggagaatt gaaattctct gctctacgtc gtgaagtgag aatccaaaat gaagacgggt
    18661 cggtttcaga tgaaatcaag gaacgtacct atgacttaaa atccaaagga caaggacgca
    18721 tgattcaagt aagtattcct gccagcgtgc ctttgaaaga gtttgattat aacgcacggg
    18781 tggaacttat caatcccatt gcggacaccg ttgctactgc cacctatcaa ggagcagatg
    18841 ttgactggta tatcaaggca gacgatattg tgctgacaaa ggattctagt tcattcaaag
    18901 ctcaaccaca agcaaagaaa gaaccgacac aagacaaata gtcgctaggt agaaaggaga
    18961 ctttttcgca tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga tttagtcttt
    19021 cattttacga tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg
    19081 aagacaatcc agcagatcaa ctggcaggat tttaacctat cacaagcaga taagattgac
    19141 attccctatt taattatcag tttcagtgtc gcaattctta tctgcttgct ggtagcgttt
    19201 gtattcaaac gggttcgcta tgatacggtt aaacaacttt accaccgtca aaaactggca
    19261 aagatgatac ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt
    19321 aaagatagtg ctggtcgtac aaaggaaaag ataacctact tccctaaaat gtattatcga
    19381 cttaaaaatg gcttgataca gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa
    19441 ctcttacact tggaaaagaa attagagagt ggcttgtact gtgagctgac ggataaagag
    19501 ttaaaggatt cctatgtgga atatactttg ctctatgaca ccatagccag tcgtatttct
    19561 attgatgaag tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa
    19621 tatgataagc tccctcatat gttgattgct ggtggtacag gtggcggtaa aacttacttt
    19681 atactgacac tgattgaagc cttgcttcat acagattcaa aactgtatat tcttgacccg
    19741 aaaaatgctg accttgcgga cttaggttct gtgatggcaa atgtctacta tagaaaagaa
    19801 gacttgcttt cttgcattga aacattctat gaagaaatga tgaaacgtag tgaggaaatg
    19861 aagcagatga agaactataa gactggcaaa aattatgctt acttaggtct cccggcacac
    19921 ttcttaatct ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc
    19981 gcagttatga ataagctgaa acagattgtc atgttaggtc gtcaagctgg cttctttcta
    20041 atactggctt gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg tgatcagttt
    20101 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
    20161 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
    20221 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
    20281 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
    20341 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
    20401 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
    20461 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
    20521 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
    20581 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
    20641 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
    20701 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
    20761 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
    20821 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
    20881 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
    20941 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
    21001 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
    21061 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
    21121 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
    21181 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
    21241 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
    21301 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
    21361 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
    21421 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
    21481 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
    21541 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
    21601 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
    21661 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
    21721 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
    21781 tatgaatttt ggacaaaacc tttgtgcgac tcgttcctta gtgaaaagct aaggcactaa
    21841 taaaagaacg aaaaagtttg gttattagga gaactgataa tctgacaggt ggaatgatga
    21901 agtaacgctc tgaaacgcct gccctgagcc tccgacaagc attgtatgag gaaactcaga
    21961 atgtttgaag ctcggtaaag acggctgaaa gataccgtaa tagttgatga acatatatct
    22021 tctatcgaaa actatccaga ggtgggtagc gtatccaaca ggtcgggggt ctataaaata
    22081 tctatggtta gaatgttcta taaggaactg acgaaacagc gaatgtacgg gtctaaattt
    22141 gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat
    22201 aggttatgaa atgccatata acgttacagg cggtatcaag ccagcaggct tatagctagt
    22261 gacctaagga tatatatgta tagataagat tatcggaacg aggaaaggta ttgggttctc
    22321 atcaagagaa taaactgacg aagaacaata agcagttgaa ctaatgctga aaagcagagg
    22381 gcgaacctaa gaaattcttg taatgagaat ggaggaatgc cctcaagtcg tgtatatatt
    22441 agaaaagtat tatgttgttt caataaggat tacgagtaag ataaaaaagc tcactcaatt
    22501 tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga
    22561 tttaagacac gctgaatatt acgaaatgca aaacaccttt gatgatttat atgccaaaag
    22621 taagaatgga gatattttca ctcatcttat ggatattatc ctatcgagag aaaatatatt
    22681 actggcatat cgaaatatca aagcaaatgc aggcagtaaa acagcaggaa cagacggtac
    22741 aataattaaa gatattggca aattgccagc agaaacagtt gtcaaaaaag taagatacat
    22801 tgttgcaggc agtccacatg ggtatcgacc taagccagtg aggcgaaaag aaataccaaa
    22861 accaaatggt aaaaccagac ctctaggtat tccttgtatg tgggacaggc ttatacaaca
    22921 atgtataaaa caagtattag aaccaatctg cgaagctaaa tttagtgaaa acagctatgg
    22981 ttttagacct aataggtcag ttgaaaatgc aataaaagca acctataacc gcctacaaat
    23041 cagccagcta cattatgtga ttgaatttga tattaagggt ttctttgaca acgtgaacca
    23101 ttccaagctg ataaaacaga tatgggctat gggaatacgg gacaagcact tgatattcat
    23161 tttaaaaagg atattaaaag ctccaatcaa aatgactaac ggtacaataa catatccaga
    23221 gaaaggaact ccgcaagggg gaataatatc cccactacta gcaaacattg ttcttaatga
    23281 gttagaccat tgggtagaaa gccaatggca ggaaaatcct gttacaaaaa actatgtggt
    23341 tcatatcaat aaaagcggta gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac
    23401 aaagttaaag gaaatgtata tggttcgtta tgcagatgat tttagagttt tctgccgcta
    23461 caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa tggattgaac aacgattaaa
    23521 attggaagta tcacaagaaa aaacaagaat tgtaaatgtt agaaaaagat actccgactt
    23581 tcttggtttc aagattaaaa tgataccgag aagaaagaag ttagttgtta aatcacatat
    23641 aagtgataaa caattcaaaa accaaaaaga caagctggta tcacaggcaa agaaaattgc
    23701 ttctccctca aaagggaaaa cggaattaga agaaacaaga ctttataatt caatggtatt
    23761 aggtatgcaa aattattatc agattgcaac gaatgtaaat ctggattgta tgaaactgaa
    23821 tagagctgta atggctatct tcactaacag attaggtaca aacaaaaggg gcagattgag
    23881 aaaaacaggc agacaactta cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct
    23941 tcggtatgtt gctggcgagg gcgaacctat tttccctgtt ggatatatcc aacacagaaa
    24001 accaatggca aaggtgtatt tagcgaattg ctacactcta gagggaagaa aatttatcca
    24061 caccaatcta agtgttgata aattactgat gtaccagtta atgaaattat ctttggaaaa
    24121 tcgcacaata gaatacgcag acaatcgaat ttcgctattt tctgctcaac atggaaaatg
    24181 tgctataact ctggaagaat ttcaacaagc taatgaaata cattgtcatc atattgtacc
    24241 aactggagct ggtggaaatg acgattataa aaatcttatt ttggtaaaag aggcggtaca
    24301 ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa tatatagctc tattgaagcg
    24361 aaataaagtc cagctcataa gactaaataa atatagagta ttagctggca atcaagaatt
    24421 aaacttgata taggtctact acataaatac ttttcaatat aaacacgatg gaacgcggag
    24481 tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa gtttgagata tttataatat
    24541 caggaactta cctatccata tataactggt ttctatcaaa cgctcaatca ctggtgcttt
    24601 tagcaatcgt tgtgattggc ttgtatcttg gcttcaagcg tgagtttagc aaactgattg
    24661 gctttttaat tattgcgatt attgcggttg gcttagtctt caacgctgct ggagtaaaag
    24721 acattttact agagctattc aatcgcatta ttggtgctta aataaaaccg ttcttttgtg
    24781 gaatataagt ggttttctta tgttccgcaa aggaatggta caccaaacga agtgcggtag
    24841 ggatttttga atctctacaa agaaaggacg tgaatatatg gacgatatgc aagtctatat
    24901 tgcgaattta ggcaaataca atgagggcga attggtcggt gcgtggttta cctttcccat
    24961 tgactttgag gaagtcaaag agaaaatcgg cttgaatgat gaatatgagg aatacgccat
    25021 tcatgattac gagttaccct ttacggttga cgaatacact tctattagcg aactcaatcg
    25081 cctatgggaa atggtatcgg agttgccaga agaactacaa tcggagctat ctgctctgct
    25141 cactcatttt tcaagtattg aagaactaag cgaacatcaa gaagatatta tcattcattc
    25201 cgattgtgat gatatgtctg acgtggcacg ctactacatt gaagaaacgg gtgctttagg
    25261 cgaagtgcca gccagtcttc aaaactatat tgattatgaa tcctatggtc gggatttaga
    25321 cctttcggga acgtttatct caaccaatca cgggattttt gaaatcgtct attaaatctg
    25381 tcggtacatt cttactggca gattttcttg tttacggggt agtcaaaaga ctatccccat
    25441 ttttatgaaa ggattgatta catgaagaaa atacgaagct ataccagtat ctggtctgtt
    25501 gagaaagtgt tgtactccat caatgacttt agacttccgt ttcccataac ctttacgcaa
    25561 atgacatggt ttgtcgtgtc actctttgca gttatgatac ttggcaactt gccccctctt
    25621 tctatgatag agggagcatt tctcaaatac tttgggattc ctgtggcttt cacatggttt
    25681 atgtctacaa aaacctttga tggtaaaaag ccttatggat ttttgaagtc tgtcattgct
    25741 tatgcactgc gacgaaagct gacctatgca ggaaaaaaag taacgcttgg cagaaaccag
    25801 ccacaagaag ccattacagc agttaggagt gaattttatg gcatatccaa ttaaatatat
    25861 tgaaaacaat ctggtctgga ataaagatgg ggaatgttac gcttactatg agcttgttcc
    25921 ctacaattac tcatttctaa gtccggaaca gaaaatacaa gtacatgatt ctttcagaca
    25981 gcttatcgca caaaatcgtg atggcaagat tcatgcttta caaatcagta cagaatccag
    26041 catactttct gcacaagaac gttccaaaaa tgaagtcact ggaaagctca aaacggttgc
    26101 ctatgacaaa atcgaccaac agacagacgc tttaatatcc atgattggcg aaaatcaagt
    26161 ggactaccgt ttctttatcg gttttaagtt gcttctcaac gatcaggagt tttctatgaa
    26221 aagtcttacc gttgaagcaa aaaatgcttt tactgatttt gtctatgatg tgaaccataa
    26281 gctgatgggc gattttgtta gtatgagtaa tgatgaaatc ctgcgttttc agaagatgga
    26341 aaagctttta gaaaataaaa tctcccgtcg tttcaaaatc cgaagattag ataaggatga
    26401 cttcggctat ctgattgaac acctttacgg acagacaggc actgcctatg aagagtatga
    26461 gtatcctcta tcaaagaaaa agctggaaca tgaaacactg attaaaacct atgacctcat
    26521 taagcctact cgctgtctgg tggaagaaaa acagcgatat ttgaaaatcc agcaggaaga
    26581 cgaaaccgcc tatgtagctt actttaccat caacagtatt gtcggcgaac tggacttccc
    26641 gtcctctgaa atcttctact accagcaaca gcaatttacc tttccgattg atacgtcaat
    26701 gaatgtggaa attgtagcga atcgtaaagc cctatctact gtccgcaata aaaagaaaga
    26761 actgaaagac ttggataacc acgcttggca aagtgataat gaaaccagct ctaatgtggc
    26821 ggaagctctg gaaagtgtga acgagctgga aaccaattta gaccaaagca aggaatctat
    26881 gtataagctg tcctatgttg taagggtatc agcaaatgac cttgacgaac ttaaacgtcg
    26941 ttgtaatgaa gtgaaagatt tctatgatga tttgagcgtg aaactggtac gaccttttgg
    27001 ggatatgctg ggcttacatg aagaattttt acctgccagc aaaagatata tgaatgacta
    27061 tattcaatac gtgacctctg atttcctcgc tggtttaggt tttggtgcta ctcaaatgct
    27121 gggtgaaaat gaggggattt atgttggcta cagcttagat actggacgca atgtctatct
    27181 gaaacctgct cttgccagtc aaggggttaa gggttcagta accaatgcgt tagcgtctgc
    27241 ctttgtcggt tcgctgggtg gtggtaaatc ctttgcgaat aaccttatcg tctattatgc
    27301 agtgctttat ggggcacaag cagtgattgt agacccaaaa gcagaacgtg gcagatggaa
    27361 agaaaccttg ccagagattt cccatgaaat caatatcgtc aatctgactt ctgatgagaa
    27421 aaacaaaggc ttacttgacc cttatgtaat tatgaaaaat accaaagatt ctgaatcact
    27481 ggctattgat attttgacat tccttacggg gatttcctct cgtgatgggg aacgcttccc
    27541 aatccttaga aaagccattc gtgcagtaac caatagtgaa gtgcgagggt tgatgaaagt
    27601 gattgaagaa ttacgggttg agaatacgcc actaagtacc agcatagccg accatatcga
    27661 aagttttaca gactatgact ttgcccatct gctttttagt gatggttatg tagagcagtc
    27721 tatcagcctt gaaaaacaac tgaacattat acaggttgcc gacttggtac ttcctgacaa
    27781 ggaaacttcc tttgaggaat ataccacaat ggagttacta tcggtagcaa tgctgattgt
    27841 cattagtacc tttgcgttgg actttatcca tacagaccga agtattttca agattgtaga
    27901 cttagacgaa gcatggagct tcttacaggt ggcacaaggg aaaacactat ctatgaagct
    27961 ggttcgtgct ggtcgtgcta tgaacgctgg ggtatatttc gtgacccaaa atacagatga
    28021 cctcttagat gaaaaactga aaaataacct cggcttaaaa tttgcctttc gttccactga
    28081 ccttaacgag attaaaaaaa cccttgcctt ttttggtgta gacccagagg acgaaaacaa
    28141 tcagaagcga ttgcgtgatt tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg
    28201 tgtcggtgtg atacagttcc accctgtatt tgaagaactg ctccatgcct ttgataccag
    28261 accacctgtg cgaaaagagg tgtaaatgtg aaaccatcaa tattaaacag aataaaatca
    28321 aactggacgc tgaaacgtct aggtaaagtg gcaatgacag tggctttcac acttgtgatt
    28381 gccatttttc ttttagccat gctgggaacg gtggttcaag ctgcgggctt ggtagatgat
    28441 acggtcaatg tggcaaatga atacagccga tacccacttg aaaactatca actggatttt
    28501 tatgtggata atagctgggg ctggctaccg tggaactggt cggacggaat tggaaaacaa
    28561 gtcatgtatg gactatatgc cattaccaat tttatttgga caatcagtct ttatgtttcc
    28621 aatgcgacgg gttacttagt acaggaagcc tattccttag acttcatttc cgctacagca
    28681 gattccattg gcaagaatat gcagaccctt gcaggggtta cgcctagtgg actatcaaca
    28741 gagggtttct atgttggatt ccttttactc ttgattttgg ttcttggggt ttatgttgcc
    28801 tatacaggac tgataaagag agaaaccaca aaggcaattc atgccctcat gaattttgtg
    28861 ctggttttta tcctatcggc ttcctttatt gcctacgctc ccgactacat taaaaaaatc
    28921 aatgactttt catcagacat cagcaacgct agtttgtcac tgggtacaaa aattgtcatg
    28981 ccccattcag atagtcaagg caaggacagt gtggacttga tacgtgacag cctattttcc
    29041 atacaggttc agcaaccgtg gctactgctt caatataaca gttcagacat tgaaagtatt
    29101 ggtattgacc gtgtggaaag cctgctctca accagtccag attccaacaa tggggaagac
    29161 agagaaaaaa ttgttgcgga agaaattgaa gatagaagca ataccaatat gacgattaca
    29221 aagacgatta accgtttagg tacagtcttc ttcctatttg tcttcaatat tgggatttcc
    29281 atatttgtat tcctattaac aggaatcatg attttctcgc aggtactttt tatcatctat
    29341 gctatgtttc tgcctgtgag ctttatttta agcatgattc catcatttga tggtatgtca
    29401 aaacgagcca taacaaagct ctttaatacc attttgacac gagctggaat cacattgatt
    29461 attacgacag catttagtat ttcaaccatg ctctatacct tatcggctgg ttatccgttc
    29521 tttttgattg cttttctaca gattgtgacc tttgcaggaa tctactttaa gctgggcgat
    29581 ttaatgagta tgttttctct acagagtaac gattctcaaa gtgtgggaag tcgtatgatg
    29641 agaaaacctc gtatgcttat gcacgctcac atgcaccgtc tacagcggaa acttggacgc
    29701 tccgtgactg ctttaggggc tgggtctgcc attgccagtg ctacgggtaa aaaaggacag
    29761 tcgggttcgg gaagttctgc gagtacacaa gcagatcact ctcgaccaaa cgggcaggaa
    29821 aaatcaacac taggaaagcg tatcggtcaa accatcggta cagtagctga taccaaagac
    29881 agaatggtag atactgctgg taatttgaaa gaacaggtta aggatttgcc gaccaatgca
    29941 agatatgcag tatatcaagg aaaatctaaa gtgacggata atgtccatga tttaaccaga
    30001 agtatttctc aaaccaaagc ggataaagcc agtggacgca agaaacagca ggaacaaaga
    30061 cggaaaacca ttgcggagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca
    30121 gcttcttccg ttcatgaaag accagccact aagcaagaac aatctcatga tggacagact
    30181 tcaaggcaaa caactgtaca ggcttcctat agggaatctc aacaagccaa acaagagcgt
    30241 ccaacagtta agtccgattc ttcaagttta aaaacggaac gtcaaagtaa tacagttcaa
    30301 gaaagaacgg ttcaaaagcc agtaacttca actgcaccaa cagatagagc ttcacaacgt
    30361 tcaatcacaa aagaacgtca gtctactgtt caaagagtac cactacaaaa tacaaaaagt
    30421 agaccaccaa tcaaaaccgc caccattaag aaaggcagta agaaaccatg aagctgaaaa
    30481 ctttagtgat tggtggttcg ggattattct tgatggtctt ctcactgctt ctgtttgtcg
    30541 ccattttatt ttcagatgaa caagatggcg gtttttccaa cattcactat ggcggtgtgg
    30601 atgtttccgc agaagtgctg gctcataagc ctatggtaga aaaagttgcc aaagaatatg
    30661 gaattgaaga atacgtcaat atacttcttg cgattataca ggtggaatcg ggtggtacag
    30721 cagaagatgt tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag
    30781 aagaatccat taagcaaggt gtgaagtatt tcagtgagtt attagccagt agcgaaaggc
    30841 ttggtgtaga tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttaggat
    30901 atgtggctaa tcgtgggaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt
    30961 attcgggtga cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct
    31021 ggcgatacaa ttacgggaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa
    31081 cagagtttga tgatgataca gtacaagcca tcatggacga agctctgaaa tttgaaggct
    31141 ggaagtatgt ctatggcgga gcttccccga ctacctcttt tgattgtagc ggactgacac
    31201 aatggacgta tggaaaagct ggaatcaact taccacgaac agcacaacag caatatgatg
    31261 taacccggca tatcccatta ttggaagcaa aagccggcga tttggttttc tttcattcta
    31321 cctataacgc tggctcttat attactcata tcgggattta tttaggcgat aaccgtatgt
    31381 ttcatgcagg cgaccctatc ggttatgccg acttaacaag ctcttactgg caacagcatt
    31441 tagtgggagc aggacgaatc aaacaataag aaaggacgat tcaatgatga aattcggaaa
    31501 aaatcagaat acagaaaaac agacaccaaa ggaaaagaaa cctcgtgtct ataaggtcaa
    31561 tcctcgtaaa aaggttgtga ttgtcctatg ggtactttta ggacttagtt tcagctttgc
    31621 gatattcaag cactttacag ccattgatac ccataccatt catgaaacca ccatcataga
    31681 aaaggaatat gtcgatactc atcatgtgga aaattttgta gagaactttg cgaaagtcta
    31741 ctattcatgg gaactaaacg acaagtccat tgataatcgc atagaaaacc tgaaagcata
    31801 tctgacagag gaacttcaag ctctgaatgt cgatacagtt cgtaaagata ttcctgtatc
    31861 gtcttctgta aaaggatttc agatatggac ggtagaagca gatggcgaca atcaatttga
    31921 tgtaacctac agtgtagacc aactcattac agagggggaa aatacaaaga ccgtccactc
    31981 tgcttatata gtgagtgtct atgtagacag tactggaaat atggtcctca ttaagaatcc
    32041 gaccattact agcataccaa agaaatcaga ctataaacca aaagctattg aaagtgaggg
    32101 tacggttgat tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct
    32161 ctatcctaca gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc
    32221 aatcggaaaa gagtacatct ttcaagaact ggtaaatcct atttacaatc gtaaggataa
    32281 tcaagtcacg gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt
    32341 atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca
    32401 aatattggta cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg
    32461 agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt
    32521 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa
    32581 ttggatgtcc ttttttagga gggcttagtt ttttgcaccc agtttaagaa tacctttatc
    32641 atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata
    32701 aaaatcccag tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa
    32761 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcgggaa
    32821 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
    32881 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
    32941 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
    33001 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
    33061 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac
    33121 ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg
    33181 atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac
    33241 agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg
    33301 atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag
    33361 aagcattgga actcgaacaa gaggaaagca taagatttca gaattgctcc ttgtaccctg
    33421 tttatcatgg aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga
    33481 ataaatttta ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa
    33541 ttgaatattc ggaagaaaga caacgtcttg catatgtacg cctttatggc ggaatcctgc
    33601 atttgcggga ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata
    33661 cttcaataaa tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt
    33721 tgcaaaatga gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga
    33781 gagagagaat tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc
    33841 aacaaaggga aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac
    33901 aatattatgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
    33961 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
    34021 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
    34081 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
    34141 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg
    34201 cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg
    34261 attgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt
    34321 ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt
    34381 tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca
    34441 acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca
    34501 ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct
    34561 ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg
    34621 aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca
    34681 ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat
    34741 gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggaattaat tatgaagtgt
    34801 aatgaatgta acagggttaa atacaaccaa attttaattt ggctttgcgt tttatctttt
    34861 tttagcgtat taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt
    34921 aataaaccac ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt
    34981 ggaacagctg tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt
    35041 ggaattataa taaattgttt cgggtcggta attgggtttg ttggccattc tttcttttcc
    35101 ttacttatta tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta
    35161 atggttgtag ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt
    35221 ggatcgatag tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat
    35281 tatattcatt ggtcctatct tctactcatt cctatgataa caattatcac tgttccgttt
    35341 cttatgaaat tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt
    35401 atactaatgt ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct
    35461 tttcttatcg ttagcgtgct gtcattcctg atatttgtaa aacatatcaa gaaagtaaca
    35521 gatccttttg ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt
    35581 gggggaatta tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa
    35641 gatgttcacc agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg
    35701 agtgtcatta ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac
    35761 gtgttaaaca tcggagttac atttctttct gttagc
//
LOCUS       DAXWLH010000005        35624 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXWLH010000005 DAXWLH010000000
VERSION     DAXWLH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.005"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..719)
                     /locus_tag="ACGE36_000180"
     CDS             complement(<1..719)
                     /locus_tag="ACGE36_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8382612.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKV"
     gene            902..2026
                     /locus_tag="ACGE36_000181"
     CDS             902..2026
                     /locus_tag="ACGE36_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8382613.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2253..3170
                     /locus_tag="ACGE36_000182"
     CDS             2253..3170
                     /locus_tag="ACGE36_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8382614.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3163..3996
                     /locus_tag="ACGE36_000183"
     CDS             3163..3996
                     /locus_tag="ACGE36_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8382615.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4079..5197
                     /locus_tag="ACGE36_000184"
     CDS             4079..5197
                     /locus_tag="ACGE36_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8382616.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5218..7089
                     /locus_tag="ACGE36_000185"
     CDS             5218..7089
                     /locus_tag="ACGE36_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFF8382617.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            7083..8306
                     /locus_tag="ACGE36_000186"
     CDS             7083..8306
                     /locus_tag="ACGE36_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8382618.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(8392..10326)
                     /locus_tag="ACGE36_000187"
     CDS             complement(8392..10326)
                     /locus_tag="ACGE36_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8382619.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            10552..11208
                     /locus_tag="ACGE36_000188"
     CDS             10552..11208
                     /locus_tag="ACGE36_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8382620.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(11282..11635)
                     /locus_tag="ACGE36_000189"
     CDS             complement(11282..11635)
                     /locus_tag="ACGE36_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8382621.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            11827..12756
                     /locus_tag="ACGE36_000190"
     CDS             11827..12756
                     /locus_tag="ACGE36_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFF8382622.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            12767..13603
                     /locus_tag="ACGE36_000191"
     CDS             12767..13603
                     /locus_tag="ACGE36_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFF8382623.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            13851..14627
                     /locus_tag="ACGE36_000192"
     CDS             13851..14627
                     /locus_tag="ACGE36_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8382624.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            14803..15547
                     /locus_tag="ACGE36_000193"
                     /pseudo
     CDS             14803..15547
                     /locus_tag="ACGE36_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            15540..17120
                     /locus_tag="ACGE36_000194"
     CDS             15540..17120
                     /locus_tag="ACGE36_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFF8382625.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(17371..17850)
                     /locus_tag="ACGE36_000195"
     CDS             complement(17371..17850)
                     /locus_tag="ACGE36_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8382626.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17866..18618)
                     /locus_tag="ACGE36_000196"
     CDS             complement(17866..18618)
                     /locus_tag="ACGE36_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8382627.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18735..18805
                     /locus_tag="ACGE36_000197"
     tRNA            18735..18805
                     /locus_tag="ACGE36_000197"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18767..18769,aa:Gly,seq:ccc)
     gene            19166..19891
                     /locus_tag="ACGE36_000198"
     CDS             19166..19891
                     /locus_tag="ACGE36_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8382628.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            20146..20412
                     /locus_tag="ACGE36_000199"
     CDS             20146..20412
                     /locus_tag="ACGE36_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFF8382629.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            20409..20840
                     /gene="ruvX"
                     /locus_tag="ACGE36_000200"
     CDS             20409..20840
                     /gene="ruvX"
                     /locus_tag="ACGE36_000200"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFF8382630.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20865..21170
                     /locus_tag="ACGE36_000201"
     CDS             20865..21170
                     /locus_tag="ACGE36_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFF8382631.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21251..22396)
                     /locus_tag="ACGE36_000202"
     CDS             complement(21251..22396)
                     /locus_tag="ACGE36_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8382632.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22457..23104)
                     /locus_tag="ACGE36_000203"
     CDS             complement(22457..23104)
                     /locus_tag="ACGE36_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8382633.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            23298..23591
                     /locus_tag="ACGE36_000204"
     CDS             23298..23591
                     /locus_tag="ACGE36_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382634.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            23635..23946
                     /locus_tag="ACGE36_000205"
     CDS             23635..23946
                     /locus_tag="ACGE36_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382635.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23943..24567
                     /locus_tag="ACGE36_000206"
                     /pseudo
     CDS             23943..24567
                     /locus_tag="ACGE36_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24708..24866
                     /locus_tag="ACGE36_000207"
     CDS             24708..24866
                     /locus_tag="ACGE36_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382636.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            24867..25229
                     /locus_tag="ACGE36_000208"
     CDS             24867..25229
                     /locus_tag="ACGE36_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382637.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            25266..26114
                     /locus_tag="ACGE36_000209"
     CDS             25266..26114
                     /locus_tag="ACGE36_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFF8382638.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            26104..27579
                     /locus_tag="ACGE36_000210"
     CDS             26104..27579
                     /locus_tag="ACGE36_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFF8382639.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            27845..28252
                     /locus_tag="ACGE36_000211"
     CDS             27845..28252
                     /locus_tag="ACGE36_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFF8382640.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            28255..28470
                     /locus_tag="ACGE36_000212"
     CDS             28255..28470
                     /locus_tag="ACGE36_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382641.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            28474..28854
                     /locus_tag="ACGE36_000213"
     CDS             28474..28854
                     /locus_tag="ACGE36_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8382642.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28988..29143
                     /locus_tag="ACGE36_000214"
     CDS             28988..29143
                     /locus_tag="ACGE36_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFF8382643.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            29212..29685
                     /locus_tag="ACGE36_000215"
     CDS             29212..29685
                     /locus_tag="ACGE36_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFF8382644.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            29682..31376
                     /locus_tag="ACGE36_000216"
     CDS             29682..31376
                     /locus_tag="ACGE36_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8382645.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            31342..31527
                     /locus_tag="ACGE36_000217"
     CDS             31342..31527
                     /locus_tag="ACGE36_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382646.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            31531..32706
                     /locus_tag="ACGE36_000218"
     CDS             31531..32706
                     /locus_tag="ACGE36_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8382647.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            32699..34222
                     /locus_tag="ACGE36_000219"
     CDS             32699..34222
                     /locus_tag="ACGE36_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8382648.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            34278..34562
                     /locus_tag="ACGE36_000220"
     CDS             34278..34562
                     /locus_tag="ACGE36_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFF8382649.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            34549..34884
                     /locus_tag="ACGE36_000221"
     CDS             34549..34884
                     /locus_tag="ACGE36_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8382650.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            35179..>35624
                     /locus_tag="ACGE36_000222"
     CDS             35179..>35624
                     /locus_tag="ACGE36_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382651.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLL"
BASE COUNT        11932 a         5783 c         7175 g        10734 t
ORIGIN      
        1 ttcacttttc taaaatgttt ttttaagctg gctggactga taaaaaaaat ttgggctaat
       61 tgttcaagca aaaaattttc tgaaagatgt tcttctaaat atcgtatgat tggatcaact
      121 aattcatctg aataaagaat atgctttttt ttaggaatga ctttctcatt tgaaggtatt
      181 gtcaataaag caagttcctg ttgataatgc cttggtaagg aagacaattg aattttttgc
      241 agacaataag taactaataa ggaaagcaaa tgaatgctca tctgttggca tagtatgaaa
      301 aaagaattga tttcctgatc ataggaaata gctggcttac tatgttttag ataatcaatt
      361 ttgaactgtt catttttcag aaaagtaatg atagatgtta tttcttgatc tatagaaaaa
      421 gattgtcccg taatatgtgc taataattgc tcatcaaaat aaaactggat aatgataaaa
      481 gtttgtgttt ccttttgagt agaaaagatt tggacttctt gctgtttatc aatgaataaa
      541 agggaaggat tatctagttc tatagattta tgtgcaaatt cagcatataa attagcgctt
      601 tcatttatta gtagtcgaaa atagggagca gcagataaat gaagatgggt atctatggaa
      661 taggttagtg tttttttgac aagattggag gattggttgt ttgttaacaa aagaaccact
      721 ttcctttctc tttttatata aaaagctttc tatttataat taatgactat tataacaaaa
      781 atgaaaagcc aaaacgacaa gtaaaatatt tttttatata aatatttgtg ttactaattc
      841 cgttaatata cataagtgaa agcgttttat ataagaactt gaaaagaaag aagggataga
      901 catgaaaaaa agatacaaat tggtgatagc tttgctaaac attaccctat tattcagctt
      961 aagtgcatgt ggaaaaacta acgaagaagt aacagcaaac atcgcagatc caatgataaa
     1021 aaaagccact acattagaag tatatgtcgc aaacggcaaa ctgacaaact atgttgaaaa
     1081 tgctgcaaaa atctataacc gcaaaactgg agcaaatatt gacttaaaaa ttgtgaatgt
     1141 agcttcggga acagcaacag tacaaatgat caccccaaaa ttggtaacac acgaagatat
     1201 gccggatatt atctccatgg cagacacttc agcagcaggt gtgctggaaa aatttcaaga
     1261 tgctttttat agtgctgatg aatatggatt ttatgacaaa tatggcgatc agttttatga
     1321 tcaaaaattg aatattttgc gtcaacaaac gagtaatgaa acgattattc cttgggctag
     1381 tgattttacc ccagcacttt gttattatca accaaaaaaa tttgctgaaa taggtgtaga
     1441 ttttgaagaa atcagttctt gggaagaata tatccaggtg gcaaaagcat taaaagaaaa
     1501 aactggtagt tttggaattg ccttgccaga atctggggac caagaattgt ttatcaacat
     1561 gctagcccag cagaatcaat ctttgcttga tgaagaagga aatatccgtc tgaatactaa
     1621 agaagcgaaa catgctgcaa agttaatcaa agaaatgatc gactcagata ttgtacattt
     1681 ctacggtgca caagatgctg agaaagcatt tcaagaatct gcaatgtttg ttgctggcgg
     1741 ctggtatgct acgaatatgt ctttgaattt tcctgatgca tctgacaaat ggcgcatggc
     1801 tccactgatt cctttttcaa aagaaaatcc aggaaaaggt gcagtatcag gaggatcaag
     1861 tttttatgtc cctaaagatg ccaaaaatcc acaagtggca caacaatttc ttacatttat
     1921 gctgacagat gaagaatgct tagccaacgc actggagctg ggcgtggcta catctaactc
     1981 attggcctat caaacagaag cagctgagaa aaagtttgac tactaaggag gacaaaaata
     2041 ctatgaacta ttgaataaaa tcaatgaaaa tacatcaaat actgttttac cagccactta
     2101 ttcagatgct acgtcttatt tgactaccgc tgcatataac tattggcaaa caggtgattt
     2161 tgaaaattca tatctaaaag aagcagaaaa ttttgctcat aagtacggcg taaaattaac
     2221 tggtgattaa aaaatattag gaggacttaa cgatgataca atcttcgtta ccaagcaaga
     2281 caataaagaa cagcaagaaa aaagcaacag atacgtctca taaaaaaagt gcttatggat
     2341 tggtcacacc ttttgttatt ttctttattg tgacgatttt gattcccatc attatctcgt
     2401 ttgcttttag ttttatgaaa attggctact catttgaatt tgttggattt gacaattatc
     2461 tagctatctt ccgagatcca ttgtttatta aatcttacac gaatgttttg attatcatgg
     2521 caggatcaat tccaataacg atgctattag ctatttactt tgctgtttta ttgaattcag
     2581 cacagttaaa aggaaaagga ttttttagaa ctgtctatta tattccaacc attacatcaa
     2641 cggtggctgt agcttccgta ttcatgactt ttttcaatcc gactgggtta ttcaatggat
     2701 ttttgaatgc gctaggaatt aacggtgtac agtggttaac aaatcctttt tggatacgaa
     2761 tatcaatgat catcacgatg atttggctta gtgtaggata taacactatt ttatttttag
     2821 ctggtcttca agggatttct aaagagatat acgaaagtgc tgaaatagat ggagcatcga
     2881 aatttcggca attcttttta ttaacgatcc ccatgctaaa gccaatcatt ttaatggcca
     2941 cagttttagc cactatcaat ggcttgggta atttcaatat cccaaatata ttctttggga
     3001 cttccaatgg accagaaaat tcagctttgg tcgtaggagt caatttatat aaaacaagtt
     3061 ttgaaatggt tgattttgga aaagcctctg ctattgcctg gacgatggtg tttgtatctg
     3121 ttatattatc ggctattcaa tttaaattcg gaggtgataa aaatgactaa acaaaagata
     3181 caaagcagaa tttttacgta cgtcattatt gtgtttggtg ccttaatatt tttgatgcca
     3241 tatttatata tgattgtagc ttcaacgcaa agtaatcaag aaattttaag tggcggaatc
     3301 aatttcaagt ttggggatat gctattaaaa aactggcaag ccatcatggc gagatataat
     3361 tatcccttga ttatttggaa ttcgattgtt attgctgtaa tcagtacagt tttagctaca
     3421 gccagttcta caatggcggg ttatgcatta gcaaagtatc gttttatggg gaataaactg
     3481 ttgttcaata cagttatgct atctaggatg gtgccttttt ttaccacatt gattccttta
     3541 ttttacatta tgagtcgttt aggattatcg aatacttatg caggtgtgat catcccttcg
     3601 attgccagta caacttcagt gtttatgatg cgacaatata gcagtcaatt tcctacagca
     3661 ctgatggagg ctgccagaat cgatggtgcc aatgagtggg tgatttttac gaaaatcgct
     3721 gtaccgattt tatcacctac gattattact agtggtttac ttatctttat gggaagttgg
     3781 aatcaatatc tatttccatt agtcatgctt tcagataaag ataaatatac ggttccctta
     3841 attatcaaaa atatgacggt ttctagtaca ggtgagccaa ttaattatgg cgcaattatg
     3901 ctaattttag caacttcagt gatcccaatg gtcttgattt atgtgtgggc gcagtctaaa
     3961 tttaaggaga atgacattgg agctgcaaat aagtaaaaaa aataacgaat gttaaccatc
     4021 tagttcgttc taggagcaac tccacattca taaaaattaa aagaatggcg ggatatccat
     4081 gaatgaacag acgaaggaat ggtcaagaaa aattttgaat gtagcttctg aaaagataga
     4141 tgcggaatta gacagaatgg ggaataaaat tccatatatt tcttacaacg gaaaatatac
     4201 agacgtaata aaggacagag gaatcgactg gtggaccaat ggtttttgga gtggtatttt
     4261 atggcagctc tttcacaaga caaaacgaga aaagttcaaa gaggcagcaa tacaacaaga
     4321 acaattatta gaatctgccc tgtaccattt ttcagacgtt catcatgatg taggtttttt
     4381 attcttacca tcggctattg cacattatca acagtctcaa aacaaacata gtcgacaact
     4441 agggttacat gcagctacgg tacttgctgg acgctataat atttcaggga actatttagt
     4501 agcatggaat gaccataaac ctggttggat gattattgat agtatgatga atttaccatt
     4561 actgtattgg gcttcgaggg aaactggtga tccgaggttc tctaaaattg ctcggcagca
     4621 tgcagataca acagctaagt atctagtgag gaaagatgga tcaaccgggc atatcgcaga
     4681 atttgatgca gaatcaggga aatatattcg gttacttggc gggcaaggat acaatgagaa
     4741 ttcttgttgg agcagaggaa atgcatgggc tttatatgga ttcgcgttaa gttatcgtta
     4801 cacaaaaaat ccgtactatc tagctatagc agaaaagata gcagtaaatt ttattcagaa
     4861 aatcgaaaaa actggttata ttcctctcat tgattttgct gtaaaagatt cgaatattga
     4921 taccagtgct ggagtatgcg ctgcatgcgg tcttctggaa ttagcaggat atgtttcctc
     4981 tgtacgatca aaaatgtatc atgaagctgc tatcaatata ctccaaaaga cagaaaagca
     5041 ctatgcaaat tggaatatgg atgaagatgg gatcattgga ggcggagcag aagcatacca
     5101 tagagaactg acttataatg tgccactgat ctattctgat tattttttta ttgaaggctt
     5161 acttcgatta gaacagtcgg aattagctat ttggtagtaa aaagaggaga aaatacaatg
     5221 aaacatccag taccactatt aaggaccaga caggattact ttgacttatt taaaaagata
     5281 aatcaaccac tccagccgtt ttatcgagca agtggcgcac aaattgatta tatacatcaa
     5341 ggggtaggat atggaaataa aattgcagga atggaaggat tctctcgggt tttatgggga
     5401 gcggggcccg caattgacca attagatgaa cgatggttga aattaattct atcaggaata
     5461 aaagctggaa cagatccttc ccatcccgat tattggggag aaatacatga aagagaccag
     5521 cgtatggtag aaatgcccgc aatgttatta gcgttatatc atcatgaaca attattatgg
     5581 aaaaaattag atacaggaga aaaaagccag attattcatt ggttctcgca aatttttgaa
     5641 tatgaatgtg cagatggtaa ttggcaattt tttaagataa ttgtcggaac aattatccaa
     5701 aaatttggtt atccagttaa agaatcagca ctgaaagaag catttgataa tatagaaaat
     5761 tgttatcttc aaaatggctg gtatcgagac agttcaagag gtcgccaaga ttattataat
     5821 ccttttgcat ttcattatta tggtttgatt tatagcgtac tagaaccaga aaagcccatt
     5881 agccagatat ataagaaccg agctattgat ttttctagag actatattca tttttttgcg
     5941 gaagatggtg caaatgtgcc ttttggtcga agcatgattt atcgttttgc agtttcaagc
     6001 ttttggtcag ctatgttgtg ggccgatctt ttgcctttta aattaggtga aatcaagggg
     6061 ttagtcaatc ggaatataag atggtggatg caaaaagaaa tatttgatga aaaaggtatc
     6121 ctgaatattg gttataccta ccctcaattg acattgactg aaccctataa tagtacgtta
     6181 tctccattat ggctgaataa gattttttta ttgttggcgc tacctgaaga ccatgagtat
     6241 tggacggcta aagaaatgcc tatgcctatt cgtgaaggaa ctaaacttct ttcagaagcc
     6301 aatttgctca tacaacatga taatggacat acattttttt tgaatgcggg acaattaggg
     6361 ccaaatttcc atacattgac caatgaaaaa tatttaaaat ttgcttattc tagtcaattt
     6421 ggtttttcga taccgcgagc gaatcaattg aaagcagaaa tggcaatgga ttcaatgcta
     6481 gggattcagc gagcggacac atatatcacg accagtttta agaatgagag catcaaaacc
     6541 tatggacagt tcatcgttcg aaataatgta cgagacattg tatgtaagaa agaggtactt
     6601 gcctctacat gggaacctac tttacaggca aaaatcagaa cttggcttat tccttttgaa
     6661 ggctggcaga ttcgcgtaca caaagtagaa ttagatacag aatatgttct atatgagaca
     6721 gggtttgcta ttgaatgttc tcctgagaaa gaaggaataa tcatagaaga agacaaggaa
     6781 aactataggg tgagtgaagc tggcttttca ggtattatct gtttaagtga cgatactatt
     6841 aaacgaaaat ccacagctat tatgggtttg cccaatacaa acttgatgac atgggaaaac
     6901 acttttattc ctggattaga gggtacattt ggaaaaggaa cccattggct cggaacagga
     6961 gtggttgctc atcaagatcg ggattatagt ttagaagtat ggaacaatcg tcccaaaatt
     7021 gattttgatg ggacaaccgg gctaactatc caaaataaaa acaataaaaa aagactaaaa
     7081 ttatgctaaa ggctggtggg gaaatgcttg ataaagtgat gtttgaagag ataatacatc
     7141 cagaaaaatg gataaatgaa tgttataaag aattaatgga aaaagaatgg aataatgaga
     7201 tcgagcgttg tcgcagacac acggagaggt taatcaaacg tatcagtaac agtcagtatc
     7261 aattcccaag cgagtgtcct ataaaaggac gttatccatt aagagacaat attggttgga
     7321 cagaaggatt ttggacagga attaattggt tgttatttga gaagacgggt gatcctttat
     7381 ttcaacaaac agctgaaaaa gcagatatag attttatcca caggattgac tgtcaaatta
     7441 tcactaatca tcacgatctt ggttttttgt attctttatc aaccgtagca gattataaaa
     7501 taacgggaaa tcgaaaaatg aaggaagctt caattgatgc tgcagaaaca ttacttcagc
     7561 gtttttggga gaaggcagga gttatacaag cttggggaga tcctagtgat ccttcacaac
     7621 aaggtagaat gatcatggat tgttgcatga atcttccatt attgtattgg gcgagtgaag
     7681 aaactggaaa tcaaaaatat aaaaaagctg ctcaggctca tattcaaaat gcagcagatt
     7741 atttagtaag agaagatggt tcaacttatc atacatttta tatggatgtc aaagatggaa
     7801 aaccaagata tggaagtacg catcaaggtt atagtgataa ttcctgttgg gctagaggcc
     7861 aagcatgggg gatttacggt tttgcattaa gttatcgtta tttgaaagaa caaaaatatt
     7921 ttgatttaag caaaatatta gccaattatt ttatgaatcg gttgccagaa gataccattt
     7981 gttattggga tttgatcttt gatgattcta gcagagaaga aaaagataca tctagtatgg
     8041 tgatcatggt ttgtggatta ttagaattaa tagaatcagg tctattagac aaaacagaaa
     8101 agaacttata ccagtctgct gtttggaaaa ttatgcgaca attgaaagaa agatatgcta
     8161 caacggaaga agaagatggc tttttaacac agtctgttta taataagccc aaaaataatg
     8221 gggtaaatac accttgtatt tggggtgatt actattattt agaagcttta gtgagaatga
     8281 ctagtaattg gtcttcttat tggtgaaaaa ggtaaatatg gaatttttgt ttgataaaat
     8341 aaaaaaagaa aaaccaaaac agcatgaatg gtttctgttt tggttttttc gttaattttc
     8401 atattcttct aattccaagc ttagttcttc ccattccgtc aattttgttt cttgatcttg
     8461 tctagcagct tctaaatctt ggttcagctc attgagtttt acgtgatctt ccaggttttc
     8521 tggcgcgacc atttcttgtt ccaactgatc gatccattca tcgatccgcg ccatttcttc
     8581 ttcaacctga gtaatttttc gttgaaggct acggataagc ttttgctgtt ctttgctttg
     8641 gtaataatca gatttgttcg tgggagcagg agcagattct ggctcatctt gtgccaacaa
     8701 ttcggcgatt tcttcttctt cctgcttttt ctctaaataa taatcatagt ctcctagata
     8761 gactttgctg ccattttcag agagttcgac gacttttgtc gcgatccgat tgatgaaata
     8821 tcggtcatgg gaaacaaaaa gcagtgtgcc gtcataatcg atcaatgcat tttctaatac
     8881 ttctttgttg tcaatatcta aatggtttgt tggttcatcc aaaatcaaaa aattttcttt
     8941 gttcatcgct aacttggcta aagcaacccg tgctttttca ccgccactta gcagagggat
     9001 tggtttttct acatcttcgc cagaaaataa gaaagcacct aaagcacttc ggatctcttt
     9061 ttctggagtg gtaggatgct catcccacaa ttctgctagg atcgtcttgt ttgtatgcag
     9121 atcagcttga ccttgatcgt aatagccgat ggtgacattt gtccctagcg tttcttttcc
     9181 acggataaaa gggatctggt gaatgattga tttcagcaac gtcgatttcc cgataccatt
     9241 cggacctact agagcaatcg cttcttgacg acggatatct agattgattg gttcagataa
     9301 ggtgacatta tcatatccga tagcggcatc ttcgatttgc aagactacat ttccagaagg
     9361 cttttctata ctgaatagaa agtttgcttg tttttcatcc ccttgaggac gatccagccg
     9421 gtccattttt tctaatgtct ttctgcggct ttgcgctcgt tttgtggtag aggcgcgaac
     9481 aaggttccga gcaacgaagt cttctaattt atggatttct gtttgctgct tttcataagc
     9541 cttccattca cttgctaact gttctgcttt caaatctaga tatttcgaat agttaccttt
     9601 gtaatggcgc atttttcttc tgcttaattc atagacttca ttgactactt tatctaaaaa
     9661 ataacggtca tgggaaacaa tcaataatgc accagtatag ctttgcagat aattctcaag
     9721 ccaggaaagt gtctcgatat ctaaatggtt cgttggttca tctagaatca aaatgtcagg
     9781 gtgttgcaaa agcatgcgag ctaaagctaa acgtgtgcgc tgtccgcctg ataaagtatc
     9841 gattttctga tcataaaatg aagaatcgaa tttaaaccca tgcaatacgg aacgaatttc
     9901 attttcataa ccatagccgt ttcgatctga aaaatcgtgt tgcagccggt cgtattcttt
     9961 taaataagaa ctataagcag gtgtgtctgg cgtaccttcg ctaatgaaaa tctctagctc
    10021 gcgcatccgc tcttccattt gtctaacttg ttcaaaagct ttcagcatct cgttccaaac
    10081 tgtttcattg gaatcaagtc ctgtgtcctg agcgaggtat ccgagagtcg ctgttttatt
    10141 tttagcgatc tttccttcat caggtgcttc aattccagca atgatcttta ataaagtcga
    10201 ttttccagcg ccatttctgc caactaaagc gacacgagcg cctgtactga tttctaaatg
    10261 tatgttttca aataagactt ctgcaccaaa atggcgagca atctgattgg cttgtaataa
    10321 tatcatggaa tcctcttttc tactttgtga attctcctca aataaaaaac tatttttcaa
    10381 ttagtagtct accataaatt ccattatact gcgagatttg cagaaatgat aaataaatga
    10441 ataaagaata aaatttcaca atatctgagc aatggaattg attttttcct ataaatactg
    10501 ctatgatgga gaaatcaaag atgtaattag acaatacgtg gaggtcattt cgtgaaagat
    10561 catattattc caaaggcgac agctaaaaga ctgcctttgt attatcgcta tttaaggatt
    10621 ttaaataatg ctggaaagaa aaaagtgtct tcaactgaat taagtgaagc cgttcaagta
    10681 gatagtgcaa cgatccgtcg agatttttct tattttggcg aattaggaaa acgtgggtat
    10741 ggttatgatg tagaagatct tatgcacttt tttgcaaaga cattaaacga cgatcaattg
    10801 acaaatgttg cgctgatcgg tgtagggaac ttgggaagtg ctttactaaa atacaaattc
    10861 catcaaagta atagcatacg tatcagttgt gcttttgatg tgaatgagga aatcgttggg
    10921 cgcatcgtag acggtatccc cgtttatcca atggaagata tgtcagaaca aatcaagatc
    10981 caacaaatcg aagtagcaat tttaacgata cccgctgaaa gagcacaaga agtagtcaat
    11041 cagttaacgg aagcaggaat caaaggaatc ttgaacttta ccgctgctcg attgaatgct
    11101 ccgtcagatg tattgatcca aaatgttgat ttgacgaatg aattgcaaac cttgatctat
    11161 ttcttgcatt ctgacgcaaa tgcgcattcc gaaaatacag cagaataaaa ataaaaagcg
    11221 attgaacaaa tgaaggcaaa actttcactt gttcaatcgc ttttttctgt ttggacaacg
    11281 attattttgt tcggaagatt tcttcataag aaagggaatc atcttctgct gtcagttcat
    11341 ctagtagata acggattttt ttaaagtcga tgaaccgtgc ttgccgaaag acttcttttc
    11401 cttgatgaaa aatcaggaca actggcgcag tcagcacttc aaatttacta gctacttctg
    11461 gtgtttcaaa tgcatccaaa tgaagtgccg gaatatcata atgtgtgaga agttcctcta
    11521 gtcgtggacg aacagcgtga caaacactgc aattaggcat cgaaagataa atgacggcaa
    11581 cggatgactg ttgcagtatg tcttgaactt cagtcaagtt agtggtttgt ttcattttta
    11641 ttctcccttt tatgatagaa gtgatatgta tgagacctta atttaatggg aataacagaa
    11701 aaagtcaatt gaagtgcttt ggtaaaaaat aagagttgtg taaaaattta cagagcaact
    11761 tgtttttttt gaataaaagc gtatatttgc aaagcgaatg aactagaaat aaaaaggagg
    11821 aaactcatgt tttcttttat tccaaattcg gttttgcaga tgagtacgat tttcttatcg
    11881 atcgttattg aagcattacc tttcgtatta ttaggctgtc ttatctcagg cgccttacaa
    11941 gtatttttga ccccagaacg tgtgaagaga tggcttcctg aaaatcgtat cttctctatt
    12001 tttactggtt cgatcttagg atttttcttt ccttcatgtg aatgcggcat cgtaccgatc
    12061 gttcatcaat ttgttaaaaa aggcgtacca gttcacacag cgtttgcttt tatgctgact
    12121 gctccgatca tcaatccaat cgtgattttt tccactttta tcgcatttgg caattcttgg
    12181 cagatggcaa gctggcgggt gataggcagc ttttttgttg cccttgtgat tggtgtctgg
    12241 cttgcctatt ttcaaaaaga cgctgtattg aaagaaaaaa tcacgtctaa agtagcagaa
    12301 aatcaccatc atcatgtcca tgaagaagaa caaagtgaga agaatgaacc aaaaaatcag
    12361 ctgatccatc aaactgggca tgtgttgaca cattcaatcg acgaattttt tgatacaggt
    12421 cgttatttga tcattggcgg attaatcgca gctgcgatgc agacttattt accaactaga
    12481 attatgctaa cgctcggctc aacaaagatt ttagccatta tcatcatgct gttattggcc
    12541 tttacgatgt ctctttgctc agaagcagat gcgtttatag gatcttcttt gttaagtctg
    12601 ttcggcacag cacctgtagc tgcctttttg gtctttggcc caatggtcga tatcaaaaat
    12661 ctactgatga tgaaacgata ttttacagga cgctttattt tatcgctgat cggactgatt
    12721 gcccttagcg ttattggatt cacattattt atttaggagg gaaaatatgc tgcggttttt
    12781 gattttatct gggtatttcg aattgatgat gtacttgcaa gtttctggca aactcgatca
    12841 atatatcaat gtccattatc gctacttagc tattctttca atgattttat ctgctctttt
    12901 ggctttagtt cagctgtatt tgtgggtcaa aaacggaaaa gcagaagaaa aggcacacga
    12961 gcacgatcat gaccatgggt tgtcaaaacc ttatcaacgt ggaatcgctt atgtactgct
    13021 agctttgcca gtaatcgtgg ggacgttgtt tccaacagtt agtctcgaca ctacgatcgt
    13081 agaagccaaa ggatttaatt tcccagtaag taaagaatca gtaggggatc cggagatgca
    13141 gacacaatac ttaaagccgg ataccagtat ttatttcaac aaatcagact acttagacca
    13201 aatgaatgag ttgaaagaaa agtatgggga taagcaaact atcaagatca cagatgaaaa
    13261 ctatttagaa gttatggagc taatttataa ttaccctagc gaattcattg gcaaaaaaat
    13321 cagctatgaa ggatttgttt accaaacacc gaacgatagc aatacagata tcttcttgtt
    13381 ccgattcggc attatccatt gtgtggcaga ttcaggagtc tttggtttgt tgacgcatat
    13441 gccggaagga gtaacggtta aaaatgatga atggtataaa atagaaggaa ccatccaatc
    13501 agactattat caaccattca agcgtgaaat cccatctgta gttgttacaa acgctgagaa
    13561 agtctctgct ccgaaaaatc agtatgtgta tcgttctttt taaaagaaca aaataaataa
    13621 acatagctca gacagctgac acgcaaggac gtcggttgtc tggctttttt tgcatgatgt
    13681 attcatttgt ttgatattta ttttagaagc agcatcctga aagtatttga tatcggtttc
    13741 aaaggtgttg gaaaagattg tgaagagaat tacaatttat tcctaacatg tatgttgaaa
    13801 agttgtatga aatgattttg atagtaaaat atcgagtgga ggtacttttt atggtaacgc
    13861 gtatatttat tttggatgat agtttggttt ttgaaaaaat gctagtagaa attttggaag
    13921 aatccagaat tttattaagt ccttggaaat ttgagatcgt tcagggattg aacgttatac
    13981 aatttgttga attcattaaa aataatgaaa tctattcctc agatattttt ttcctagaca
    14041 tcgatttgaa cgcctattat agcggtatcg aactaggcga aatgcttaga gaaaagaatg
    14101 aaaagtgtaa aattgtttat ctaacgtctc atccgaataa agcaataact gttattaatc
    14161 gaaagattac gcctagtggt tatttgataa aagaattcac ttatgaaaaa aataaaaaaa
    14221 gcatccaaag ttacttattg aaagaaaaag aaaactatcc tgttcaacat gctacgaaaa
    14281 aaaggtggac aactgtccgt tatggtaata gcgatcacta tatccattac gatgaaatcc
    14341 tttatctaat gagcatgcca ggttataaaa ataaaatagg cctagtattg aaagatgaag
    14401 agattctcgt aaacggcaca attaaaaaat ataaaaaatc actcgaatat gagtatttat
    14461 gtaaagattt aaaagcttat atcattaata ctgaaaacat tacatctatt agcagagtaa
    14521 acgaaatagt aacatttgca aatggagaac aactagagat gggaaagaga agtattgata
    14581 aactaaagaa ttttatgcaa gaaaagatag aaaatgaatc attttaaaga aaacaaagac
    14641 tcatatctta cctgttagaa gagtgttttc ctgtagaaga gaacaaccca agtagcagcc
    14701 tagtcgtttc tatccttttt tgttatgcac tctttttgac atttactcca ccaataacga
    14761 agggtacaat aaaaagtaat aacaaacaaa aggagtacag aaatgattga attcatcaat
    14821 gtaagtaaaa gttacgggaa aaaacaagca ttaaaagact tgtctctctc gataagacaa
    14881 ggggaaattt ttggattttt aggacataat ggtgccggaa aatcaactac gattaaaagt
    14941 ttagttagta ttattgaacc atcatcggga acgattttag tagatggaat gaaattgacg
    15001 gaaaatcgat tgagtatcaa acaaaaaatc ggttatgtgc ctgattcccc agatattttt
    15061 ttacaattga cggctggaga atattgggac ttgatttcag ctgcctatga attagacaca
    15121 caaaaaaaaa gaaaagcgtt tagcagaatt gacagcgctt tttgatatgt atagtcatca
    15181 aaatgaaaca atcgcaagtt tttcacatgg aatgcgtcaa aaaacgattt tgatcggcac
    15241 gttgctgcca gatccagata tttgggtatt ggatgaacct ttgcaaggac ttgatcctca
    15301 agcagcattt gatttgaaag aaatgatgaa ggctcatgcc gcaaaaggaa aaacagtcat
    15361 tttttctaca catgtattgg ataccgcaca acagttatgc gataaactgg ctattttgaa
    15421 gaaaggtgaa ttgatttatc agggggcagt tagtgaatta ttgaatggct cacctgatga
    15481 aacgctagaa aagatttatt tgaaaatggc tggaaggcaa gcgacaggag atggaccgta
    15541 tgtataagcg acaattatta gaattaattc gagttaatct tttatatgtt aatccacagc
    15601 tcacgaataa agcaagagag aaaggaaaaa agggaaagcg attaactatc tatcttttgt
    15661 ctcaaaatct tttttcaggg ctgatttttt tactgattta cggatttgct atgtttgcta
    15721 tgaactttgt taaattgcca ggtttcttta cttattacgt ggcacttttt ggtatgttag
    15781 gtttaactca aagtatatca gtcatttata atatcttttt tgaaggaaat gacctgcaag
    15841 tatttttgcc tctgcctttt ggacaagaac aggttttttc agcaaagatt ttggtagttg
    15901 ctttgaccat cattcctttt gtttttccgc ttctagtcat gtttatttta accggatggc
    15961 gagctgatct agggattttg cttactatca ttatctcaat ccttttgttt tctctatttt
    16021 tgattattct tttttgtctg gctagtttga ttgtttttgg tttggctcga acagcagtgt
    16081 ttaaaaaaca tagaaaaaca gtttctagta tcttgcttgg cagctcgatg ctgatcgcat
    16141 ttgctggaat catgtggatg aatcatcaaa cggggactat tgaaaatgag ttaacagatc
    16201 ggcatccgat ttcgatatta ttgccattct atgatgctgc cagtcagcca ctttctcaac
    16261 aaggtttatt gggttgggga atactagcta tcacggctct gggatttttc cttgctgtaa
    16321 aacagctgat agtaccaaag cttacagagc aactgactga ttcaaacagt gagttcaaaa
    16381 taaaaagaac ccataaaaag aaccaaaatg tgcatcagct tttatttagt tacaatgctc
    16441 aattagtaaa agaacctaat ttgattatgc aagttctttc ttcttccttg ttaactccaa
    16501 tcatttttat tcttgctttt gccctaggag gcgaaataaa attaactgat ttagataata
    16561 agttaatcgg tgttgttttc ctggtaggga tcgcattggc tttcttaaca gttaatcaga
    16621 cttcttttat cagcaatctt atttctttgg atcaagaaaa ttttctgttt atccgttctt
    16681 tgccaatctc tatgaaccaa tatttaaaag aaaagttcag attcggatgg atattgcaga
    16741 gttttttaac tggtgggatc gcattattaa gcggactgtc ttttcagctg cctttgtttt
    16801 ttatccctag cttgctattt ggttctttat tcggctgcta cttattgtca ctacgttatt
    16861 tcgctagaga ttatcggttg ctgctgctga actggaccaa tattaaccaa ttatttaaca
    16921 gaggttcagg aagtttgggt ttagtcgcaa ctatgatggg ttcattgatc atcagtatta
    16981 ttcttatcgt cttatacgga atgttggctt tgttttatcc attttggtta gtgaatattc
    17041 ctgtgatcgg tttagttcta atactttccg ctctatggat cgtttattat caacgaacgt
    17101 tttggaaaaa atttgaataa agaagagggg ccgtgacaaa aatcatgtcg cagccccttt
    17161 tttacagata gagggtgttc aaaagtcagt ttctcagtat taagtcaatt ttatgtaaaa
    17221 atgtgagaag ctatttttac aaaacttttt ttaatactca taattacttc ttattgctcc
    17281 tgcggttact tgtcgcaatg tttgtgagat attttgtcat cgctcaaggc tgaacgtctc
    17341 tttcacaacc ttttctttgt tacacttcgt ttatttaaaa agccgttttt ttcgtttcat
    17401 catgattgcg tcacgcttta ctttgaaatt acggatgtta tggttaaaga cttctgataa
    17461 tacaagacct aaagcgattg ctccagcaat cataattgct tggaccgcat aatgagccgc
    17521 ttctcgatag gattcagtaa cgagatttcg aaccgcttga taagctaaac caccagggac
    17581 tagaggaacc atcccgggaa tattgaaaat cgttacgggc attttcaaac gtttagagaa
    17641 aagatcgctg atgaatgcta ctcctaaaga accaagcaat gaaccaaaag cagcagatcc
    17701 gcctatttga acacagatcc agtagatcat ccagccaaaa gcaccagaaa gaccacaaca
    17761 aatcaatgaa cgcctaggta catttgtaat aacggcaaat gccgcagtcg ctaaaaaact
    17821 aaaagaaaac tgaataaata tcgtccccat acttgtgttc ctttcctaat aaaaaagctg
    17881 gaaaataaaa gcaatcgcaa aaccgatcat cgtagcagtc atcatcgctt ctgttccgcg
    17941 agaaactcct gagacataat gtccagctag caaatcacgg atagcgttcg taatttgaac
    18001 tccaggaact aaaggcatca cacatccgat aatgattaga tcctgattgc ttcctaagcc
    18061 aaaacgtacg ctgtagatag cggctatgcc aatcaataaa gaagataaaa attccgataa
    18121 aaatttgatt ctaaaaaatt tcaaactata tagataagct gcgtatccca atcctccaat
    18181 gactaaagtc acaggtaaat cggtcatctt tccgccaaat aacaccataa ttgttccact
    18241 gatcactgcc gcgctaacga tttgcaacca gacaggaaag aaagaacggt ctcggtcgat
    18301 tgcttgcagt tcgcagtaaa gttcttctaa tgttagctct gaagcaacat atttacgtga
    18361 aagatcattt acttttacta ctttttctaa attaatcgaa cggttggtaa tttgttccat
    18421 tcggatggtc gaggtacgat caagaccaat aaagagaccg gtttgagtga catagctgac
    18481 caaacggaaa tttccagaag ctaaagcaat tcgactcatc gtatcttcta cccgatacat
    18541 ctccgcattg ctttccatca tgattttccc agcgagtaaa caagtgtcca gcaacagatc
    18601 taattctttt cccgtcatag catcttctcc aataattgat tgattagttt tattttacta
    18661 caaaacatac cagaagcgta aagaataaaa aaacttgagg aaggaataaa agaggcgtat
    18721 actgtttttg tattgcgggt gtagtttagt ggtaaaatcc cagcttccca agctgatgtc
    18781 gcgggttcga ttcccgtcac ccgcttaaaa taggttgtga aaaagctgct ctgcaacaag
    18841 taataagaac agccaagcaa aaacaacttc ttatgttttt aacttggatg gacttattac
    18901 cgcagatgca agcttttgcg caccgtttac caagcggttg tgaaatcagt gtattgtctt
    18961 gagtatgaag aaaatttttg aataataata aataccttac agatagagtg gtgactgatt
    19021 agagacgagg aatccgtctc ttttttgtat ttttaagcca tcttaaaaca ggttttattt
    19081 tttgctgttt ctatttcttt ctagtattta tttatagggg aacatatgtt aagatttagg
    19141 gagctgttag taaaggagta aagaaatgag caaaaaaaca accaaggcga tcgtggggat
    19201 cgtcgctgct gtgattgcag gggcattcgg tgtttccacc acacaaaaag attcagatat
    19261 ctttcagcag attacaacta tttttgattc cacaccacaa gcttctcaag tagcggatgt
    19321 gccagaatat gatggaaaac atcaagaaat agaaatcaat gggaatcagc cggaatttac
    19381 aaaagaggat cttagccttt ccaaaggaac atgggaaagc tattcaaata ttgataaatt
    19441 gaacagagta ggacaagcca atgctatgct tggaaaagat ttgttcccta ctgaaaagag
    19501 agagcctttg tatattgacc cgacaggttg gaaacaaaag aaattaagtg acgggcaatg
    19561 gttatataat cgcagccatt taattggttt ccagctgact ggtcaaaata acaacattaa
    19621 aaacttgatg acagggacac gttcgttgaa tgctccatac atgcttgctc acgaaaatga
    19681 tattgcggca tatatcaaag aaacaaatca tcatgtacgc taccgtgtca ctcctcattt
    19741 tgaggggaat gaactggtag ccaggggagt gcaattggaa gcagaaagca ttgaagacaa
    19801 aaaaattgaa ttcaatgtct ttatctacaa tgttcaagat ggctacacga tcaattatga
    19861 gacaggacaa gcaacaaaaa caaaaaatta gtagcagcta ttcgaatttt ttatgaatat
    19921 tatgaaaaaa ttgtgtaaaa acatacattc aaagaaatat agcgtatact ttatgaaaag
    19981 agtacttttg ataaagctac agaaaaaagg aaaaaaacat acaaatagtg tttcttaatt
    20041 tattcggaag tttggaatat ttgtttgtag ttttcccttt cttcgagtaa aataaggttg
    20101 tagaaggtaa gctggaatta ccttgcggga aagaggtgta gaatcatggg ttttacagat
    20161 gaaaccgtac gttttggttt tgacgatagt cgcaaaaaag aagtgagcga aacattagcg
    20221 attgtttatc gggctttgga agaaaaaggc tacaatccaa tcaatcagat tgtcggctac
    20281 ttgctttctg gagacccggc ctacattcct cgttatcgtg atgctcgaaa tttgattcgc
    20341 cgtcacgaac gcgatgagat catggaagta attgtcaagg actacctctc aaatcatgga
    20401 gtgaatttat gagagtaatg ggcttggatg tcggttctaa gacagtaggt gttgctatca
    20461 gtgacccctt tggctggaca gctcaaggag tagagatcat tcggatcaat gaagcagaag
    20521 aagagtttgg ctttgatcgg ttgaaggaat tagtagcaca atatgaagta gaccggtttg
    20581 tagttggatt acctaaaaat atgaacaatt ccatcggacc aagggcagaa gcttccatgt
    20641 catacggaga aaagatcaaa gaactattct ccttgccagt tgattatcaa gatgaacgat
    20701 taaccactgt tcaggcagaa cgtatgctag tagagcaggc aaatacttca agagccaaac
    20761 gaaaaaaagt catcgataaa ttagcagctg tgatgatctt acaaaattat ttagacggct
    20821 caggtaagca attattttaa taataaaaga aaagaggtaa tcttatgaca gaacataacc
    20881 attcccacga ccatgaagga cacgaacaca tcacattggt ggatgaccaa ggaaatgaaa
    20941 cattatatga aatcctacta acagttgacg gacaagaaga atttggacgt aactatgttc
    21001 ttttatatcc tgctggtgtt tcagaagatg aagatgtaga attacaagct tatgcataca
    21061 tcgaaaacga agatggaaca gaaggcgagt tagaacaaat cgaaacagac gcagaatggg
    21121 acatgattga agaagtattc aatacattca tggcggaaga agaagaatag actataaagc
    21181 taggagaaac cttgatatga tagggtttct ccttttttta ttttttactg tatgccatat
    21241 tgtatgccat ctagtcaata tttaagtgtt gtactaattt ttcaacagtc ttttctttcg
    21301 tttcttttgc caagtgaccg taacaatcga tcgtctgact aatttttgta tgccgtaatc
    21361 tagtttgaac ttctaccata gtagctccag cgtccaagag cattgaagca tgagtgtgtc
    21421 ttgctacatg cacaccgaga aatggaacca tagctttttc acaaatagta atcaatcttg
    21481 cataaatagc gtggtgtgtg attagttctt tatagatacc acaaaaaatc atagtcggat
    21541 cagttacacc aagtgtcagc atatatttcc gctgattaat ctgccatttt tttaatatag
    21601 aaattgttct ttcatctagt ggaagaaccc cagcactaca atcagtttta ggatcactga
    21661 ttttatagcc atttttggat tggcttagtg ttttattgat ggtgaccgta ttttcttgaa
    21721 aatcaatgtc tgaccaattc aatgcaaggg cttcactgat cctaaccccg ctataaaaga
    21781 gaaagcgtag taacgtttta tcaaagtcgt ttcgataagt ttcaggtttg ctgtttaggt
    21841 aatcaaacaa ggtgatcagt tgttctttac tgtaaacttt gaccttttct ttatgcttag
    21901 tggtggttct tttaggcatt aatacgttgt ctagtggatt agtctcaata atcccaagat
    21961 tgatagcgta gctaaaaata cgcttagaag ttgaatgaag taccttgtat tggtcagtag
    22021 tttcagccca gctatttaca atttgttggg ctttttttac cgtgacttca ttcacacgaa
    22081 gatttccata ttttggaaga atccactttc taaacttgat ttcagttttc atgaaggtgg
    22141 cttctcggac agtggtttta tatgtttcta gccacaagtc ataaacttct ttaaacgttt
    22201 cgttgttcgc ttttttaata ccgtttttat cgtagtccac ttgcaagcgt gacaaggcaa
    22261 gctgggcttc tttttttgtt ttgtaacctc gtctacgaac cttatcaggt ttgccagtca
    22321 ttttgttcat tccaagatag gcttcataga gccaaaattt acctttttta gtttcatatt
    22381 gcttgaatgt tgccataata gttttccctc catagtgtag caagccgtgg gggcggttgg
    22441 aggaggcagt ttattattat tttgaatagt gtttatttag ctcagtaaca agagctgtga
    22501 actcttttag aagatcattt tcataaaatt cttgatactc ttttttctca tctgtatttt
    22561 tttctgaatc attaattaat gagtcatatc gtaaaagcgt atttagaaga gctgcgagat
    22621 aagcaattgg ggtactcccg tcaggaaatt caattcgaga tcctgtttta agatatttta
    22681 ttgcgtttgt taaataaatc ttctgtgaga cagtcaggtt atcttcttcg atgttattga
    22741 tagcattaga aagattagaa tcaattatag agttattttt ttcaatagtt tttttcaatt
    22801 gcttttcaag cacatttcgc ttaatttcgg gcatcatatg catatctctt aatgtataag
    22861 atccgtttaa tagataggta gtggatacat ctaatacctt tgaaagttgt ttaagcctgg
    22921 tgttattggg aagattatag ccattttccc aattactaac ggcaccttta ctggccggtg
    22981 gggataattt acctccaagt tgttccattg agagtccctt ttcaattcta gccagtctaa
    23041 ttctttgtcc tgtctccact gcttcggggt atttttttct agccacattt tcaccaccat
    23101 tcatatattt tctctactat atcacgattt aaatatgtgt gaaacataaa agttcaaaaa
    23161 agtatgttaa aattcttgca aatgaaaagg tatgatggta tattgagttt ataagaggta
    23221 tagaaatgta ttcaagagcc tcttgcattt ttttaaaatc tttgtataga aatgtatgct
    23281 aacgaggggg gataaatatg gaattaaaaa ttgacaaact cacgcttgat gatccagctt
    23341 ttaaaattat tgacgatcga gttgttcaac aagtagagca aactcaagga acgttacttt
    23401 cattattaga aaaaaagtac cagtttccaa aatatatgaa caaggcacaa gctgctaagt
    23461 atatgaacgt ttcatacaat actatgatgc agaaatatgt tcctaacggt ttaaagttga
    23521 ttatagttga tggagtcgta agaatttcac aagatgaatg tgatcgcttt atggaagaat
    23581 accaaaaata aacgcaagcc gtgggggcaa agggattaat aggaggaaat aataatggaa
    23641 tcgacacaaa ataaagcaat cgaaaaagta ttatcaacaa ttcttacaga agacacagcg
    23701 gcagaattag caaacttgga aggcaaagcg ctagaagaaa cgtttgaatg tttgtacgaa
    23761 caaatggact atcaaaaatt gctaccacaa gaaccaacgg taagcggagt attgcgtggc
    23821 ttgaacgatt tagtacaagc agagtttaaa gaacgacttt ctattgaaga gtatcaagaa
    23881 attttgtatc agcaagttga gttgttagcg agcttgctag ggcttgaact ggaggacgtt
    23941 gaatgaaaac tgaaatttgg aacggacaca ttataagatt tgtcgatatt aatgatgaat
    24001 ggtgggcagt ggcgaaagat gttgcggaag cgttaggtct taaacaggtt actagagcca
    24061 ttcactcttt gcctaaagat ggggttacta ctagtaaggt catcgattca ttgggcagaa
    24121 cacaagatgt aaacatcatc aacgaaaaaa aatatttacc gcatggcatt caaaagccgt
    24181 aaaaaagaag ccgaggcatt tcaagactgg attttcgaca tcatcaaaga actacgccag
    24241 tccacaggac ttgaaggctt tcaagtattc cgcatgctag acaaagaaca ccaaaaagaa
    24301 gcaatgacta aattaagcca cgctattact gaaccaaaac cagtagacta cattaaagcg
    24361 aatgtgatag ccaataaagc tgtatcaact atttatggtc attccaaaat ggtaaaaaag
    24421 aaagatatga ctcctgaaat gttggttgat cgtgaaccta ttttagatga gacagtagaa
    24481 ttaatgactg ttaaagaaaa atatgggttg cagttttcag tgagtgagaa aatttataat
    24541 cgttctgctg aattgcagac aacttaggag cattaacatg atcgagaaca aagaaaaagc
    24601 cttatccata acagtcttgg cggacaggat aaaggcaact taattgggat aaaacccgat
    24661 attctttgtt ccgattatac catattaaaa aatcggaggg aaccattatg aaacgaaact
    24721 taattatagg aagcatttta atcggcggaa ttttcggctt attaccatta attgcaaaaa
    24781 tagctatctt tgcaggatta gcgttatatc tcttcactgc ttatgacgag tacgactatc
    24841 aattaagaac tggagggcat aagtagatgt acaaaacaaa actattaaac caactcgata
    24901 gccttgaatt agaggaaatt aatcaaggga tcgctgaact agaaaataat atcgggaaaa
    24961 cctactttgg aaattcattc aatgaaaaac taacagtttt gtatgtactc aaaaaacatg
    25021 cagaacacaa aataatttgt cgagaaatca acgagctaaa aaatcagatt ttaaccgcat
    25081 ggttaaacat tactgatatg caagaagcaa gggtgaaaac atttaataca tgggtaaagt
    25141 atcagaatca attaaaaggt gctgaatttg ttcgggatgg gttgaaatac gaacttgagc
    25201 agttgaagct catggaggtg tctgaatgat ttataccagt attttgaaaa aggaggagag
    25261 gtttattggc aaataattat caagcagcaa cggaattaca aaagtcggga tggtcaattt
    25321 atcccttatc tcctggaact aaaattccaa taaaaggctc tagtggttgg aatgatgcaa
    25381 ctaacgattc taatcaaatt aatgaatggt ggacggaaga tgcaactaga aatattgggt
    25441 taatgcttga agatcatcaa atggtggtag ttgacctaga tcaacattct gaaacacgaa
    25501 acggcgttga gaattataaa aaacttgctt caatgtatga tccatttcct tctacttata
    25561 cggaaatcac accaggtaaa ggaatccatt tcttctttaa aattcctgat ggtgtaatta
    25621 tccaacaaga aacaggtgca tttgctgata tcttcgggcg tgatgataaa ggtaaaagtt
    25681 taagcgggat tgatattatc actaaaggag ttccaatagc gccaactatg attatttcaa
    25741 gaagtgttgg gagagcatat gaacctattg aaaacgtatc tctaaataat attgcaccag
    25801 ctcccgattg gctagtgaac ctactgctaa aaaaaccgca agaaataaag actcaatacc
    25861 gacccgtagc aaaaacaggg acagcaaaaa aattagatat gattgttcaa ggcgctggcg
    25921 aaggtagtag aaacgatcat ttaactaagt tatgcggttg gttactatgg cacggtgtag
    25981 ataatcaaac tttagcagaa ttgatttata cagcaaatgc gtacaatgaa ccaccgctaa
    26041 aagataaaga agtgaatcaa attattcgat cgatgattaa aaaagatttg agggggaaag
    26101 ctcatggctc ataagcataa attagatagt attttagact ttcccgaagc cagcgagcgt
    26161 gaagacaata tcattgaatt gaaaacctgg atgtcacgat taagatgcaa taaagacgat
    26221 cagattaaaa gtaacagtgt tgtgaatgca gaattgattt taaccaatga tagcaattta
    26281 gctggaacaa ttgcttataa cgaatttagc ggatacattc acctattaaa agattctcct
    26341 tggatcaatc gtaccgctgg agaatgggaa gacagttttg aggatgcctt aacggcatat
    26401 attgaagaaa actacaatgt agtttttgat gacaacaaaa tacacaaagc agtagtaaat
    26461 gtggctagaa agaatgtttt caacccagta aaagaacgta tcgaaaaagt taaatgggat
    26521 caacagccta gactggaaac actgtttatt gaccttttag gggtagacga caacctttac
    26581 acacgagaag taacaaaacg ttggatagtc ggatcagtcg cacgtatcta taaaccagga
    26641 ataaagtttg agatcgttcc agttttggat ggaccgcaag ggattggtaa gagtaccgtt
    26701 ccagcattac tttacacgga tgattttttc accgattcat tagattcgct aggtgaaaag
    26761 aaagatgatt atatgcagct tcaaggcaat gtaattattg aacttggaga attatcttca
    26821 atgaacaaaa ctaaaattga acagataaaa aacttcatca gtgccaagat cgataaaatt
    26881 agacctcctt atggtcggaa tgtcatagct tgggcaaggc aatgtgtatt tattggaacg
    26941 tcgaatgatg ggcagtattt gaaagatgat acaggaaacc gccgttttta tcctttgcct
    27001 tgtaagaata agccaaagat gaatccgttt aaaacaaagg atgaatattt tcttcaagtg
    27061 ttagctgaag caaaggtgct ttttgataaa ggacaacgaa tctattttag tcccgatgaa
    27121 gataaagaag tcttagaaat tgcaaaagac tatcaagagg atgccaagat ggagaatcca
    27181 attaaagaag caatcactaa atatttggaa atggaaatcc cgtataaatg ggaagaagct
    27241 ccagcgtggg cgagacgttc ttattatcag aactatcctg acagtaaatg taatgagaaa
    27301 aatttacagc atttctataa tggagctact atgcaacaag agttttattt agttgatagc
    27361 gtactcaccg ctgatatttt ggaagctgtt tttgataaac aagcaaagga tttattgaat
    27421 ggtcgatcag atgcagaaac taaaaagatt gccttgatta ttagcagtat tcctggttgg
    27481 gaacggaagc aactgccaaa aagaaataaa cgacgaggat tttacaataa gtcaaatgcc
    27541 tatgaaaata aaaatcgagc tggaaagtac aaaaagtaac gggtagtaac gggtcagtaa
    27601 cacctagccc gttactagtt tacccttact gccccaaggg atacagcaat ttggtaacgg
    27661 ggtaacacct tatttcctta aaacttttga aatagttagc taatatgctg gggaataatg
    27721 ttactggatt ttacccgtta cctgttacca aaacgctaga acccttgtcc ccgttgggat
    27781 tgagtggtaa cgggtacctg ttacgtaggt gttaccacgt gttacgaatc aaggaggaaa
    27841 gtaaatgctc aatattattt caacaaacaa agcaccgaac tttcaatata cagatgaaat
    27901 ggatcggttt ctaatgaaca cgctggcatt tagtgtggga ttggtaacag aagattattc
    27961 cacatttgat ccagaagtat taaagataat ggaagaagaa cctgattggc tgcaggagtc
    28021 agtagcgtgg tgtcaatcgc tagtagtggg atcattggta gatagtggca actatgatga
    28081 tacaggtgag ctaatggatg aatttaattg tctgcttaac ctatatgatc gagcgagaca
    28141 acgagagctt acatcgaatg aggataattt gtttttaaac atccatgata agttcttggc
    28201 tttgctacta acagatgatg aattgataac taatttattg gaggtagaat aattatgcat
    28261 atggaaagta ttagatactt cgatggtgag aaacacattg agtattcaaa cactcaagaa
    28321 gatgtagact ttattagttt tgaggaagat aaaatcacta aagttaattt taaagacgga
    28381 acgtgcttga agatagtttc gccttatatt gaatacaaat caaaatggga acaagatgat
    28441 tctgacgaac gagatcgctt tggttggtaa tctatggcag ttaaaaaaca atgtaatcat
    28501 gctggatgta aaacattgat tgattatagg cagaagtatt gtgggaagca taaagcaaag
    28561 caaacggcaa ttaagcgtga agaaagaaag caaagcgaag gtaaatattt tcaattctat
    28621 caaagtagaa catggcgaaa ggcttcatac ttgtatcggt taaatcatcc agtatgtgag
    28681 gattgcctag aagaaggctt gataaggaaa gctgatgttg tagatcacaa aattgaacta
    28741 aaagatgatt ggaatagacg gttagatgaa actaattttc gttctttatg ccatgcacac
    28801 cataattcca agacagccaa tgaaagacaa agacgagaaa agagtaccct ttgagttaag
    28861 ggggcatagt gtgaaactta ctgacaatcg atgcctactc attttggtac aaataaccgt
    28921 tgtgaaaagg cataagggta attacaaata acgattacac ttgtaatcac aagtgaaaaa
    28981 gtatataatg aaagtaggag atttattgaa aaatacaatt gttattactg ataaaacagg
    29041 gaaacagcgc accattgagt tacctcattt tggtcaagtg accattcaaa tgcagaacgg
    29101 aaaaatcatt tatatagata aattagataa agaaaaattc tgatcgaaaa acgaaggaat
    29161 gtgagcttaa ttgcttacgt tcctttttct tttgtctgaa aggaggacaa catgggacac
    29221 cctaaaatat tacacgatac aaaaggcaat atatcaagtg aagaaaaagc aatccgtgtg
    29281 gacgctagag aagaattgtt caagcagcaa ccattaataa atatcacgcc ccctgactgg
    29341 atggcagcga gcgctagaag tgaatggaat cgtatcgtac caacattaaa aaaagattat
    29401 ccattgagtg aagcagatta tggttcattg gtagcctatt gtttagcctt tgctcgaatg
    29461 aaaacagccg aagccgagat aagaaaatca ggtacgttta tcacatgcga aaacggagta
    29521 aagaaagcca atccagcggt tcgggttcaa tcacaagcta tgagtgattt gaaaaaacaa
    29581 gccacctcac taggtatgac cttagaatca cgatcaaaac tagctttgaa caaggttaaa
    29641 aatgatgaac ccgaagaccc gttcaaagag ttgatgaatt catgaatgat tacattgaga
    29701 aagtcctatc aggtaagctg attgcaccca agaaaattat ccaagcgtgt gagcgtcata
    29761 taagcgattt ggagcgttct aagtcagata gcttccctta tgtgttcgat gaagaacaag
    29821 ccaccaaagc gattaaattc attgagttgc taccatctac ggacggtaaa gcaatcaaga
    29881 tgttaggatt tcaaaaattc attctaggca gtctttatgg ctggcgtact aaagaaggga
    29941 attacaggcg attcaatcga gcgtttacca gtatgagccg taagaatggg aaaacgtata
    30001 tcgcaagtgg catggctgcc aatgcgttga ttatggaaca agaaccagca gaagcaaggc
    30061 aagtattgtt tgtaagtaac gctttgaaac aagctaaatt gggctatgat atgctgtcta
    30121 attcacttag aaacgtggtc aagtctagta agtttttaag accacaactg aaaattatga
    30181 actctaagat tcagcacttg ccctctaatt cgttcgctat ggcactggct agtgaaacca
    30241 gcacgctaga tgggtttgca ccaacaaccg caattcttga cgagtggcac gaagcaaaaa
    30301 ctcgtaaaac gtacaacgtc attaagtcag gaatgaccca acaaaagaac ggcttattgt
    30361 gtgttattag taccgctggg cttgatttaa acgttcctat gtacgaggaa tacttattgt
    30421 tagatcgtgt gctaaaaggc gaagaacaag ccgacaggta ctttatagcg atatgggaat
    30481 tggacgatcc cgaagaaatt cacgatcaag agaaatggat caaagccaat ccgatttttg
    30541 aaagtgaaga aatcaaaaaa gtaatgattc caaccattca agatgatgtg aaccttgctt
    30601 tgaaacaaaa taaccttaat tctgtattgg tgaaaaactt caatttatgg agacaagcga
    30661 gtgaggacag ctatatgatt gctgaagact ggcaggcaac cgaagtagaa ccacaagata
    30721 ttacaggcaa gcccgtttat atcggagtgg atttatctaa aacagatgat ttaactagcg
    30781 tttcatggat cgtaccgcta gataacggcg aactttattg tgattcacat agctttgtag
    30841 ccaccaaata tgggcttcaa gacaaagaaa agcgcgacgg tttgccttat cgagaactag
    30901 aaaaagctgg tgagtgttcc attactcaat tagaaagtgg aatcgtggac tatgaccaag
    30961 tatttcagtt tattcaagat ttgattcaag aaaatgattt ggaatgtatg gggatttgtt
    31021 atgacccgta taacgctaat tcgcttatca gtaaagctga aaaagccaac tacccaatgt
    31081 tagaagtaag acaaggaacg attactctaa acgttccgac ccgaactttt agagaacaag
    31141 tttatgaagg caacgttatt cacaataaaa atacgattct cacccatgca gtgaacaacg
    31201 ctattttaaa aacggataac aacggcattc agattaataa atcaaagaac agtaacaaaa
    31261 ttgatccaat agctgcatta atcaatgcct atgtgtttgc aatggattac ttcaccacaa
    31321 cggaaggagc gaaagcagac aatgaatttt atacaagtga agaattttct ttctaattac
    31381 attcataccg ttcttttact tcttggattg gtgtgtgtgt tggttgctat caccttacta
    31441 acaaatgtct actatggctt gttagcgctg ggcatagtgc ttattggggt agcggtcatg
    31501 ctaaatacag aacagaaggg aggttaaaag atggcatttt ttaaagcgag acaaaataca
    31561 acgggagatc ctttcctaca acatgtggta tcaatccaat cggatgatta caccaccagc
    31621 tttacaagcg ttcgtgcatt aagaaatagt gatgtgtttg cagccgttcg aattattgct
    31681 agtgatattg cttcaagtcc gattcaattg gtcaaaaaca atatgccaca agctgataat
    31741 gaactggtga agttactaaa cgagaaacct aattcagaaa tggatggttg gcatttaaag
    31801 tttgctttgg cggtcaatat gttgctaaac ggtaatagct ttgcagagat taaacgcaac
    31861 ggtgaaaagg tagaagaaat tcacttatta cctaactcaa gtgtaacggt tactcaatta
    31921 gataatggta cgttgtctta tcagattggt gataaaaaaa gacgtgtgaa gtctagcgat
    31981 attttgcact tcaaatattt cacccaagat ggtttaacag gattaccacc actttatgcg
    32041 ttacgtgatg aactgaaaat tcaacaagct ggcaaccgca cattgcacaa tttctttact
    32101 cgtggtgtca gtgggtcagg tattttgaag gttcataagt cagatttaga cggatcagca
    32161 aagaacgcaa ttcgtgaaaa gtttgaagaa gccaacggtt caagtagtgg agataatgct
    32221 cttagaacga tcattcttga tgaaacaatg gactacaaaa cattagaaat aaacaccgat
    32281 gttttgaagc ttatcaattc caatgattgg aacacaaaac aaattgccaa ggcgtttggt
    32341 gtaccgattg agcgtttagg cgttgaaaat gaacattcta gcaccgttca aagtaatctc
    32401 cagtatatcc aaagtacgct gattcattac tttaatgtgt ttgtgagtga attagatacg
    32461 aaacttgaaa ccaatatccg ctttaattcc gatcagttac tagagactga tcctgaaaca
    32521 aaagtaaaaa atgtattgga tcaggtcaaa gggtcacttc tcacgattaa tgaggggcgg
    32581 tcgaaaatgg ggctaccccc catggatggt ggcgatcgtt tactagcaag tttgaacttt
    32641 acgtatttag atacgttgga gaaatatcaa ttaaaagaac aggaaggagt tacaccagtt
    32701 gaataatgaa gaagaaaagg aaaaacggct gacagaagaa gctgagctaa cagccgattc
    32761 tcccaaagta gagaaagaaa atgaaaaaca accaacagac ggcaaaacta tttcaggcta
    32821 tgcgttgaaa ttcgggcaac cgtcaaaaga tttaggcggc tttgtagaag tgattacacc
    32881 cgaagcatta aaagaggtgg atttatcaaa tgtgttctta ttatatggac acgattattc
    32941 aaagccacta gctagtgtca aagcaggtac gttgaaattg aatgtcgatg atacaggatt
    33001 acattttgaa gcagagctaa ccgacacgac ctattcaaat gatgtgtatg agaatatctc
    33061 aaaaggggtt gttgattcta tgtcctttgg gttcgtgctt ggattagatt cattcgacaa
    33121 aaaagaagat ggcacaattg aacgatcaat agataaaatc aaagcactta atgaaattag
    33181 tgtcgtgacc gttcccgctt atgattcatc aaatgtccaa gtaaataagc gttcttacga
    33241 atcgtttatg agtaacaacc aagcaaaaca aacaaacaat agcttagaat ccacttctaa
    33301 agcacaaaag gagagtaata acatggaaaa aactttaatc gataacgaaa aaacagaatt
    33361 gcgtgggtat gaagaatata tccgttcaca aggcgaagtg cgtgatggag tcactactgt
    33421 aaatgcagcg gcagttgttc ccgaagaagt aatcggtgaa gtctttgatt tgaaacgttc
    33481 aaattataac ttagctcaat atgcaacagt aaaaacagta tcaaatggac aaggtaaata
    33541 tccagtagca actaaccaac aagcagtgtt agcaacaaaa gctgaacttg ctgaaattgg
    33601 tgatattgac gctgaaatgt ttacttcagt tgattataaa gtagaaactc gtgctggtaa
    33661 gattgcctta tcaaatgagg ttgtggaaga ttcagcagtg aatattgtac aagaggtcaa
    33721 agatcagtta gcgaaattgg tagaaaacac tgacaataag catatcatgg atttattaaa
    33781 aacattcact aagaaaacgg ctgctacgtt ggacgatttg aaacaactat acaatgtggc
    33841 attagaccca gcattaaata aaatggtaat tctaaaccaa agcggatatt accacctaga
    33901 tacattgaaa gattcagatg gacgttacat tttacaaccc gatgtgacag cacctagtgg
    33961 taaatcatta ttcggtatgc cagtagtatt gattgcagat acattgtttg ccaatcctaa
    34021 agctggaaca ttccctatga ttatggggga tattgcacaa tctatctttg ttgctcgtag
    34081 aaatcaagta acgactcaat gggaaaaatt cgattactac tcacaaggac ttgcagtgat
    34141 cgttcgcaac gattataaga aaattgatca aaatgcttca gtgtatattg agtttacgcc
    34201 agttgtaaca ccaaaagtat agaaaaatat tgggcggcgg tttatcccac tgcctttttt
    34261 tcattaggag agataaaatg gtaattttgg atagcataaa aaaaagtatg cggatcgatc
    34321 acactattga tgatgacttt attcaacaat tgattgatac agcaggcgaa tatataaaaa
    34381 gtgctattga tagtagcgca acggataaag atatggataa ttatcagcaa tttgatttgg
    34441 cggtgtcatt acttactcaa cattggtatt tgaaccgtca agaagccagc agcgaacgga
    34501 taccagtaac ggtacaagca ttagtacaac aaatgagagg tgcttattat gccaatcatt
    34561 aagaatgtaa atgaattgac tgagagagtc aagtttaaaa agacaaaacg ggtaaaagat
    34621 gaagatgggc agatggtgga tagtgaagaa actgtatttg aatgttgggc taatgtgcgt
    34681 tcacaaatgc taaaagacgt gcttgctagt gtaggtacca ttcttgaagg aacgttgacg
    34741 tttattattc gatacgatca agattatgaa ctaacaaacg atatgaaagt agcttggaaa
    34801 aacaagagtt ataaaattat ttcaatcaat gaaggaacgg cgtttaaaga ttatacaacg
    34861 atcatagcta aacagatttc ttagaatgat tacacttgta aacgttgtgg gtatttagta
    34921 taattaaggt agtaaatgaa gggttagctg cctgaatttt gcaagactta gctagtcgaa
    34981 atctattgtt ggactgaaaa ttgtaggtgt ggttgcaaac atgctggact aagaaagatg
    35041 atagatgatg aacgttcaat tttaatacag gcatgctagg actgattgga tggttttggc
    35101 aagctgattg tgtagaaatc aaagaacacg tctgttgtgg acgtgttttt tttgttataa
    35161 ttcaaaagag gtgatttaat ggacaaagca gagtttacaa atttttattg gaagtattat
    35221 ctacatttag aaaatgagtt tattaatacc acagattttg tgatgctcga tgagaataat
    35281 ttcaaaacat tctctattga atatcaaaaa ctactactag cgattggttc ggagtgtgag
    35341 attattttca aagaactttg cggatttgat agcagtagca ataaaagcat aactgatttt
    35401 aaagctgtta ttaaatctag cgaattaatg tgtttggata atggtgttag ggttatgaat
    35461 tctttaacat tgacttcact aaaacctttt ggagatgggt ggcctgaaga tacaccagat
    35521 tggtggaaaa tatataacag agtaaaacat ggtcgttctt caaattataa ggaagcaaat
    35581 ttggaaaatg tgttgtatgc tttagcaagt ctatatttat taga
//
LOCUS       DAXWLH010000006        34186 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXWLH010000006 DAXWLH010000000
VERSION     DAXWLH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.006"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(59..187)
                     /locus_tag="ACGE36_000223"
     CDS             complement(59..187)
                     /locus_tag="ACGE36_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8382652.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACGE36_000224"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACGE36_000224"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8382653.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACGE36_000225"
     CDS             complement(1674..2729)
                     /locus_tag="ACGE36_000225"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8382654.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACGE36_000226"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACGE36_000226"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFF8382655.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACGE36_000227"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACGE36_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFF8382656.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACGE36_000228"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACGE36_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFF8382657.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACGE36_000229"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACGE36_000229"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFF8382658.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACGE36_000230"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACGE36_000230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFF8382659.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACGE36_000231"
     CDS             10491..12050
                     /locus_tag="ACGE36_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFF8382660.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACGE36_000232"
     CDS             complement(12161..14092)
                     /locus_tag="ACGE36_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8382661.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACGE36_000233"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACGE36_000233"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8382662.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACGE36_000234"
     CDS             complement(15021..15773)
                     /locus_tag="ACGE36_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8382663.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACGE36_000235"
     CDS             complement(15932..16141)
                     /locus_tag="ACGE36_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382664.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACGE36_000236"
     CDS             complement(16152..17480)
                     /locus_tag="ACGE36_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382665.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACGE36_000237"
     CDS             17616..17771
                     /locus_tag="ACGE36_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFF8382666.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACGE36_000238"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACGE36_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFF8382667.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACGE36_000239"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACGE36_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFF8382668.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACGE36_000240"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACGE36_000240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFF8382669.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACGE36_000241"
     CDS             complement(21455..22387)
                     /locus_tag="ACGE36_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382670.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACGE36_000242"
     CDS             complement(22544..23203)
                     /locus_tag="ACGE36_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8382671.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACGE36_000243"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACGE36_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFF8382672.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACGE36_000244"
     CDS             complement(24244..24534)
                     /locus_tag="ACGE36_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382673.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACGE36_000245"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACGE36_000245"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFF8382674.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACGE36_000246"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACGE36_000246"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFF8382675.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACGE36_000247"
     CDS             complement(28200..29369)
                     /locus_tag="ACGE36_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFF8382676.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACGE36_000248"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACGE36_000248"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFF8382677.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACGE36_000249"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACGE36_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFF8382678.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
BASE COUNT         9157 a         7457 c         6134 g        11438 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccat
//
LOCUS       DAXWLH010000007        34138 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXWLH010000007 DAXWLH010000000
VERSION     DAXWLH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.007"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            49..495
                     /gene="nrdI"
                     /locus_tag="ACGE36_000250"
     CDS             49..495
                     /gene="nrdI"
                     /locus_tag="ACGE36_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8382679.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(560..2542)
                     /locus_tag="ACGE36_000251"
     CDS             complement(560..2542)
                     /locus_tag="ACGE36_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFF8382680.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2556..3452)
                     /gene="murQ"
                     /locus_tag="ACGE36_000252"
     CDS             complement(2556..3452)
                     /gene="murQ"
                     /locus_tag="ACGE36_000252"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8382681.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3455..4516)
                     /locus_tag="ACGE36_000253"
     CDS             complement(3455..4516)
                     /locus_tag="ACGE36_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFF8382682.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4727..5254)
                     /locus_tag="ACGE36_000254"
     CDS             complement(4727..5254)
                     /locus_tag="ACGE36_000254"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8382683.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(5321..6823)
                     /gene="eat(A)"
                     /locus_tag="ACGE36_000255"
     CDS             complement(5321..6823)
                     /gene="eat(A)"
                     /locus_tag="ACGE36_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8382684.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7081..8202)
                     /locus_tag="ACGE36_000256"
     CDS             complement(7081..8202)
                     /locus_tag="ACGE36_000256"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8382685.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8328..9269
                     /locus_tag="ACGE36_000257"
     CDS             8328..9269
                     /locus_tag="ACGE36_000257"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8382686.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9419..10315)
                     /locus_tag="ACGE36_000258"
     CDS             complement(9419..10315)
                     /locus_tag="ACGE36_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFF8382687.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10584..11924
                     /locus_tag="ACGE36_000259"
     CDS             10584..11924
                     /locus_tag="ACGE36_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8382688.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12142..12585
                     /locus_tag="ACGE36_000260"
     CDS             12142..12585
                     /locus_tag="ACGE36_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFF8382689.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13089..14507)
                     /gene="nhaC"
                     /locus_tag="ACGE36_000261"
     CDS             complement(13089..14507)
                     /gene="nhaC"
                     /locus_tag="ACGE36_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8382690.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14910..16133)
                     /locus_tag="ACGE36_000262"
     CDS             complement(14910..16133)
                     /locus_tag="ACGE36_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8382691.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16164..17003)
                     /locus_tag="ACGE36_000263"
     CDS             complement(16164..17003)
                     /locus_tag="ACGE36_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8382692.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17020..17700)
                     /locus_tag="ACGE36_000264"
     CDS             complement(17020..17700)
                     /locus_tag="ACGE36_000264"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8382693.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17702..18775)
                     /locus_tag="ACGE36_000265"
     CDS             complement(17702..18775)
                     /locus_tag="ACGE36_000265"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8382694.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18831..19069)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19112..19345)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19422..20093)
                     /locus_tag="ACGE36_000266"
     CDS             complement(19422..20093)
                     /locus_tag="ACGE36_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8382695.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20180..22828)
                     /gene="mgtA"
                     /locus_tag="ACGE36_000267"
     CDS             complement(20180..22828)
                     /gene="mgtA"
                     /locus_tag="ACGE36_000267"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFF8382696.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(23170..23344)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            23517..23954
                     /gene="ndk"
                     /locus_tag="ACGE36_000268"
     CDS             23517..23954
                     /gene="ndk"
                     /locus_tag="ACGE36_000268"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFF8382697.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(23976..24842)
                     /locus_tag="ACGE36_000269"
     CDS             complement(23976..24842)
                     /locus_tag="ACGE36_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8382698.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25002..27095)
                     /locus_tag="ACGE36_000270"
     CDS             complement(25002..27095)
                     /locus_tag="ACGE36_000270"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8382699.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(27844..28467)
                     /locus_tag="ACGE36_000271"
     CDS             complement(27844..28467)
                     /locus_tag="ACGE36_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382700.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(28626..30023)
                     /locus_tag="ACGE36_000272"
     CDS             complement(28626..30023)
                     /locus_tag="ACGE36_000272"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8382701.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(30055..31026)
                     /locus_tag="ACGE36_000273"
     CDS             complement(30055..31026)
                     /locus_tag="ACGE36_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8382702.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31400..31825
                     /locus_tag="ACGE36_000274"
     CDS             31400..31825
                     /locus_tag="ACGE36_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382703.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(32313..33014)
                     /locus_tag="ACGE36_000275"
     CDS             complement(32313..33014)
                     /locus_tag="ACGE36_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8382704.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(33030..33470)
                     /locus_tag="ACGE36_000276"
     CDS             complement(33030..33470)
                     /locus_tag="ACGE36_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8382705.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(33470..34045)
                     /locus_tag="ACGE36_000277"
     CDS             complement(33470..34045)
                     /locus_tag="ACGE36_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFF8382706.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         9748 a         7160 c         5920 g        11310 t
ORIGIN      
        1 aacttaaaaa agatgctata ctaaaaggaa agaattggag gatgatccat gaacatttta
       61 tatatatcga tttcaggaaa tacgcgtgcc tttgccaaac atctagcaga atatgctgaa
      121 aaaatgcatg cagaagatcc tgtcaatcct gaagttactt taaaagaaat ccatgaaaat
      181 agtgatttta cgaaagaaac agaaccattt tttactttcg ttcctactta tttagacggc
      241 ggaaatggtt tagataacgg tgatacagaa atcttgaccg aaacgatgcg tgaatatctg
      301 gaacatgaag acaatcataa actttgtctt ggtgtagtcg gtagcggaaa taaaaacttc
      361 aataatcagt attgtctaac agccaaacaa tatgcacaaa catttggctt tcctttcttg
      421 gcagattatg aattacgggg aacaccatct gatgtgacac gaatttatca gattttagca
      481 gaaaacaata aataataaaa aagaagaagc tgaaaaatag atttcagttt cttctttcgt
      541 ttctattgtt tctatcatat taagctttga catccatcac aggtgctttt gcttttgctt
      601 tacctgtttt ttccaagaca aagtttttta cacgatccat attggttacc aagacgatca
      661 tggatgtttc tttgcctgct gctttgatcg cttctaagtc agcttctgct acaagcgtat
      721 caggtgtcac ttgctgccct tctgttactt tcacatcaaa tggcgtaccg cctaagtcaa
      781 ctgtgttgat tcccatatga agcaagattt ctaaaccatt tggcgttgtg atcccaatag
      841 cgtgtttcgt tgggaagaca gagctgacta ccccagtaac tggagaatag atatgtccgt
      901 tttctggttc tacaaagtag ccttcaccca tcatcttttg tgcaaaaact ggatcacttg
      961 ctttatctaa agaaacaact gttccgtcag caactgatga gaggatagtc acactttcag
     1021 ctgaagtttg aggtactact ttttcaggca cttcatttac tgtggctgtt tcagtcgcta
     1081 attcttcttt ttctaaacgg tctttaggaa taccgaagaa gaatgtagca acgaagccac
     1141 cagcataagc tgccaataat ccaaggatgt agcctaacca tttaccgtca gagatcaatg
     1201 ggatcaaagc ggctcctgaa ggtccaatag cgatagcccc gacatttccg atcattccga
     1261 tcactgcccc gccgatacct ccaccgatac aagcagtaat aaatgggcgt cctagaggta
     1321 atgttactcc gtaaatcaat ggttcgccga ttcctaggat cccgactggt aaagctcctt
     1381 tgatcatttc tgttaattct ttatcttttt tgcagcgaat ccataatgcc aatgctgcac
     1441 ccacttgtcc tgcaccagcc atcgcaagaa ttggaagcaa tagcgtacga ccagtttcat
     1501 caatcatttg cacatggatt ggagtcaaaa tttgatgcag tccgaacata accatcggca
     1561 agaataatgt gcctagaacg aagccggcaa atgctccacc aacgccaagt acccagttga
     1621 tcccaccgac catgccatca gaaatgaaac ctgctaaagg catgatgaag aaaatttcaa
     1681 tcagaccgat ggcaatcaat gaaagagttg gtgtaacgat gatatctacc gcatcaggta
     1741 ctactttatg cagttttttc tcaacgattg acaatagcca aacagcgaag atgacaccta
     1801 ggatcccacc ttgtccagct gcaagagctt caccagtaaa taagtttttc agtggtgctt
     1861 ccggattcat ccctgtcagc atcacaacgg ccccgataac tccaccaaga gttggtgttg
     1921 caccaaaaac ttgtgcagca ttgatacctg tatagataac taggtagcta aacatcccgt
     1981 ttttcaagat gttcatcaca tcgatatatt gttgccatgt tgctgcatca agtgttccgg
     2041 ctgtgactaa gtttgctaaa actgcggcaa tccctgccac gatcccagta ccgacaaatg
     2101 caggaatcat tggcacaaag atattagaaa tgtcttttaa taccgatttg attttagatg
     2161 gtttttgttt cgatttttgt gccgctttca tttcttgtgc ccgttcgttt acacggtctt
     2221 ttccagatgc tgatgaattt gctgtagctg tacttacaga agaaagtttt tctcctaatt
     2281 tcacgcctgc caaatcaacc atttgctgtg ctactttgtt cactttccca ggtccgatga
     2341 tgacttgaag ctgttcatct tctactacac caagcactcc tggaatcgct tttaattcag
     2401 agacatttac aagatcatca ttgatgatct tcatacgcac acgagtcatg cagtggacaa
     2461 tgctttctac attgcccatg ccgcctgccc ctttataaat ttgttttgcc agttcttcca
     2521 aagtcagttc gttttttgcc atggtctttt ccccctcata aggtcccttt tacaaatccg
     2581 tttgcttcag ctaattttgc ttgagcttcc tctttatcac tatttgtcaa aatcatgacg
     2641 atagccagct taacattttg atctgcttct tcaaagtact tttcagctgt ttcgtaatca
     2701 gcttctgtcg cttgcatgat gattcgtttt gagcgttcga ttagtttttc atttgttggt
     2761 ttgacatcga ccatcaagtt attgaatact ttacctatac caatcatact gatagtagaa
     2821 atcatattca agataagttt ttgtgctgtt cctgacttca atcgagtaga gcctgtcaaa
     2881 aattctggac ctgcatcgac ttcgattggg aaatccgcgt gtttactgat ttcggcttgt
     2941 ttattgcaag aaatcgttcc tgtgacagca ccgatcgatt ttgcgtaatc taatccaccg
     3001 atcacatatg gtgtacgacc gctagctgcg ataccaataa ctatatcatt ttttgtcaaa
     3061 ttcagcttga ttagatcttc ttgccctagc tcataagaat cttcggcccc ttcgacagct
     3121 acagtcattg cttcttctcc accagcaatt agacctatga ccatttccgg ttctacaccg
     3181 aatgtcggta cgcattcagc agcatctaac acacctagtc ttccacttgt tccggctccc
     3241 atataaatca gacgtccacc ttttttaaag gactcaatcg tgttagcaat cactggttcg
     3301 atacttgtca gttctttttc aaccgcagcg gccactttct gatcttcttt gttcattagt
     3361 tgaacggctt gagaaatact catctcatct aatccaaaag tttcttggtt tctgcgttcc
     3421 gttgttaggt tctctaaatt gattttgctc atttttatct tccttctttt cttataaatt
     3481 gatagttcat accagctttg atttggttga tcaatggtag ctcttctttg ataactttag
     3541 ctactacatt cactttttca tccgctggca aatcatattt ggttaattgg atctcaccca
     3601 tatagcgcaa atacttttcg ttatctaaag taatagaccc tttcatacgc gtagctgtat
     3661 ttcttgctgg gataggcgga atctctcgaa aacgtgcatc tacgcttcgt atgacatctc
     3721 ttgcatcgtc ttgccgattc gtatgcttcc ctaatatata ttcgtaaaat tcttcatcta
     3781 caggttccgt atggagcaag actgtctgtt ctttttggaa agaagcaaat tgctcctgaa
     3841 cgttttctga cagacctgca tctccgatgt aaacaatatc cgtgttactt gctaatagat
     3901 ctagagcgcc tgcaagagga tggatccctc gatgttcttc caaagttggc aatccttgat
     3961 atagtgggcc tctcaactct gtatctcctg gtacaaaacc ttggacaagg agaccttgaa
     4021 ttttgagcca ttgatttttt ttctgatacc attctttgtc cagccctgtt tcgggacgtg
     4081 gataataatt atgccaagct tcaatctgtg agaaatcagc ttctgcttct ttcaattcat
     4141 cgatatcttt aggcgtgatc gtgctagcat tcaaactgat tttcaattga tgtgaccagt
     4201 cggcaatctg ctgattggaa atatgataat ccattcttaa acctgttaca ccaattgctt
     4261 tcaaaggcgt tagatcttct aatgaaaaac ccgctcgttc caatgcctct cctgaaatat
     4321 cgaccatcaa ttctagttta ttctttttcg cttcgcttcc tagttcgatc aatcgctttt
     4381 gatatgccga aacatcatct tctggtatat gcatggaagt gaaaataccg acaaagccat
     4441 tggatgccat ttggcggata tactgtttct tctcttctga caaagaatcg ttcataaata
     4501 ttgaaaagcc aaacatatta tccctctttt tctgtatccg tcttcatttc tatgactaga
     4561 gtatatatct aaagaaacaa aatttcaata attttattaa aataattaaa tttcatttct
     4621 aaaggttgag tttatattat caaaagaatt gtcataacct aaaaaaggca ctgctaagca
     4681 aaagcagtgc cttgaaatat tttatttaaa taacacaaaa acataattac tcataagaaa
     4741 ccaattctgt attatctacc atgaatgtaa agaacccctc attaggcatc ggatgaaaat
     4801 aagcattgat cactcttatc aaggcattat ggcaaactaa cagatagaca ttttcttcgt
     4861 cttcgatgca ttctttcaat aaaggaacga tccgtgcata cacatctaag actgactccc
     4921 catttgggaa tcgtacagcg aacgctaaac gtgctttttg aaagttctca tctttgcttg
     4981 gcagtccatc ataatcccca aaatccattt ctaccaatcg ttcatcaacg gtcagcggca
     5041 agctgagacg atcagcaact gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga
     5101 tgattttcgt gattggaaca ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca
     5161 tctgtccttt ttcagtcagc ggcacatctg cccgtccgca aactaatcct tgtgcgttcc
     5221 aagttgtttc tccgtgtctc gttacataaa gcatgtgatt ctctccttcg ttggcaaagt
     5281 tccccttaac cataccttgt tgttcttgag attacaaggg ttagattctc tcaagagaaa
     5341 taatctcagt agatacttta tccaggaagg tttgatcatg ttccactagt aacatggcag
     5401 gtttcacgtt caagatcagt tgttctaatt gttcttgatt gaagacatcc aaataattca
     5461 atggttcatc ccatgtatat agttcagctg gctgtgataa agatttagcc aattccactt
     5521 ttttccgttg gcccatactc atctgctcga tcttgttatg aaaaacatct ctttccatcc
     5581 caagctttcg gaggttgttc aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt
     5641 ccgccaacgt tcctcgattg tcttcataat tttggcttgc ataactaatg ctcagatgtt
     5701 ttggctggta tttttctcct ataaccttgc cgttgaatgc ccccagaaga taatggataa
     5761 tggatgactt tcctgcaccg ttaggacctg aaatcgccac ccgctgatga ggctcgattg
     5821 taaaatgaat tggctcgaat aacagatttt cataccctaa ttgaagatct tctacgctta
     5881 aaagtcgctt atggtgagac gcttggctat tcatcgtcaa agaatcgata tattcgatat
     5941 cttttaatag tttttctttc tcgctgatct gggtctccat ccgattcacg attgctttgg
     6001 atcgtttcat cgtccgtgca gcatcagcac ccactgctcc tttattcact cgtctagatt
     6061 cagtatcgat gaatccgact tgtttctttg ttttatctcc ttctcgggaa cgagaccatt
     6121 cggctttttc agctgctgtt ttctttagcc tactgacttc ttttttcaat ttttcatttt
     6181 gagccatttc aaattcatca cgaagttttt tctgttcttc atagatagag aaattccctt
     6241 gataaagttc cagttgactt ttttcgattg ctaaaacatg gtccactact tcatcgataa
     6301 atccccggtc atggctgacc acgatgaagc cttgtttttt ctttttcaaa taagccgcta
     6361 cttgttttct accagagata tccaaatgat tcgttggttc atcgattaac gggaaatgag
     6421 tgtcatccac aaataaaagt gccagtagga cttttgtctt ctctcccccc gatagtgtgc
     6481 tgaattctct ccataagatt tcaggatctg tttgcatcaa ttggagctct ctttcgattt
     6541 cccatatctc aaaatccgta atatcattta acacgtaata ggttaaacgt tctttatctt
     6601 ttgtctgttg cgggaaatag gcaaattctt gctgatggat tacttgccct tgataaggta
     6661 gtttgttttg tagaatattc agtaaagttg tctttcctcg accgtttcgt ccgataagtc
     6721 ctagtttcca ttgtgtgtca aaatttagat ttgcttgttc aaataataat gtgccttggc
     6781 tgtcgtagcc gaatgtcaga ttttttattt cgattttaga cattttccat cacctcgtaa
     6841 aaaaagacca gtcctatttt cagaactggc taaagaggat gcaagggttg gatacacaca
     6901 aaaagaccca tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac tgcttccttg
     6961 tctccacaag gtacagtctg aatgcagctt tcatcaatcg acttaatttt tcaatgcacg
     7021 gatccctcac tttgtcctat atttgaaatt acgatagcat aaagaaaatg attttgtcaa
     7081 ctacttcggg aggatggtga aaaatctgct aagtgtctgt cctaagattt ttgttttttc
     7141 taatgtttgc ctgtgttcca ttgcttcttc taaacctatt ggacccaaat gaatacttaa
     7201 gacaattggg agaaaatcag aaagtccttc tagctcttgg tctctacttc cgcaaatagc
     7261 gattattggt tttcgatatt tttttgcaag cactgctaca ccgtatggaa cttttccgtg
     7321 gatcgtttgc tgatcgatcc gcccttctcc tgtaatgatg acatctgctt gttcgatctt
     7381 tttttccaat ccagtgattt ggctgactaa atcgaaacca gaaaccatct tcgctcccaa
     7441 caaaaaaagg gctccaccaa taccgcctgc tgctccactt cctggaatat catctaaatc
     7501 cacctcccag aaatctttta tttgctgagc aatctttcct gcttgtttat ctaagaaagc
     7561 tagcgtcgta tctattcctc ctttttgtct tccaaattgc caagcagctc cttgttgacc
     7621 tgtatatgga ttggtaacat ctgcagcgat aatcaatttt actttagaaa caagaacgtt
     7681 tcttcgatca aaatgaagtt ttttagtaga taataaagga tttccttctg tgtacccttc
     7741 taaagaagca cccaaggatt ggagtaatcc tagaccacca tcgctacacc cgcttcctcc
     7801 taaagtgaca ataatttgtt ctacttcttg aagcagtgca tctttaataa gttctcctaa
     7861 ggcataactg gaagcaagtc ggatagtttg atccgttggt ttatcaagta gatctaaccc
     7921 caatgcttgt gcagattcga tgactgcggt gttttttcct tgccaagttg tcaataaata
     7981 gctgattttt cgctgacgga ataagagatc catcccatca atggtacgcc atgttcccct
     8041 gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca tctgcaatcg gcacattgat
     8101 ttttcttcct ttacgatctt cccagccatt tagaactgcc tgatttagtt caaatgaaga
     8161 agcacttcct ttaaatgaat caattgccgc caaaatatcc atctgtatcc ctcactttct
     8221 tcttaagtaa gacacttttt ttcatttttt tcaaacaaaa caatgctatt ttcttccatt
     8281 tttctatata ctaaaaggga actcattgaa taaaggagag aaaaaacatg aaaatcgcaa
     8341 tagctggggc cggagcaatg ggcagccgaa tcggattgat gctacatcaa agcggaaatg
     8401 aggtcctctt gatcgatcgt tggcctgctc acattgaagc cattcgtaca aatgggttga
     8461 ttgctgactt taacggtaaa gaagtggtcg caaaactgcc aatctattca ccagaagaaa
     8521 tcatcgaaag caacgaacac gtggacttga tcgtagcatt aacaaaagcc aatcaattag
     8581 atgatatgtt ttgttcgatc caatccatta ttactgacaa tacttatgtt ttgtgtttac
     8641 taaatggatt aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa aatatcttat
     8701 ttgggatcac gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg
     8761 atggagaaat cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg
     8821 tagaagtctt tcaagaagca aatttaaatc cgatctatag ccataacgtt cgttattcca
     8881 tttggcgaaa agcttgtgta aatggaacat tgaatggttt atgcacgatt ttggattgta
     8941 atattgctga actaggcgcg caaaaagcgg cagagagcat ggttcgaaca atcgtatctg
     9001 aatttgcttc tatcgcagca aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac
     9061 atattgcttc tacttatgat cctgacaaca tcgggttgca ttacccatct atgtaccaag
     9121 acctgatcaa aaaccatcgt ttgactgaaa tcgactacat caatggtgcc atttggcgaa
     9181 aaggacaaaa atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg
     9241 caaaagaagg aatccttggc gcagaataaa aggctctctg atgagaatca gctgaatcag
     9301 cagctggttc tcttttttta agcccaataa aagatcaaaa agaaaacaca actctcatga
     9361 tgccataaga attgtgcttg tttgtttcag tacataatct cttcaaaatg taggtttttt
     9421 atttgttttc aacagaggag aggtcatatt cgtattgctc ataaatagtg gaatcgttag
     9481 tgataaatcg ttttgcccaa ttttctgcat cataaaattc caatagctct gataggattg
     9541 gcttaaatgg tgtatgcaga aattccggta attcatctaa atcacgttct cgaagcaaaa
     9601 aaatattttc aggacgataa aaatgataat gagcaatcgc ttgattatcc gtcactagtt
     9661 tcttttgaaa atacatggat tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca
     9721 acagttccag aatcccgcgc gattcccctg tttttttcag gtactcttca taagtaagat
     9781 atccaggttg ttcattccct cgatgtggaa gaatgaaata gttggtcgta acatctcttt
     9841 ttataaattc ctgcttatag ctttctgctc gttcttttct cccattattc gttgcaccaa
     9901 aaaatagatc cgtctttggt tcgctagaag aaaaattcat atgtttcgaa taaaatgaac
     9961 tattatgttt cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa atctcatcga
    10021 ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc
    10081 aaaaaaataa cacaattttt gcattggtaa atgggcgcac gcgtcgagcc atctccatat
    10141 caaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt ctagctgatt
    10201 taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag
    10261 gcaaaaaata aagtgattta ttttttgcaa ctagcagtac gtcatttttg ttcatctttt
    10321 ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag
    10381 taaaacgttt cctatatttg tatgaaaatt taacatttgt atgaaaatat gatgaaataa
    10441 atcattcttc tattaaaatt cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat
    10501 ttgttatcat tagtcattgt taaatattgc tatttattct taatataaat tttgattgtg
    10561 aatttaaaag aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca
    10621 gacatatcac catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg
    10681 gcgaagcgat tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggtgggctga
    10741 ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc
    10801 acggattatc tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt
    10861 gggtctactg gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct
    10921 ttttgcagtt gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt
    10981 taactacgat catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg
    11041 cttttgctaa aatcagtgtc attatcttac tgatcatttt tgctacttat cttgtcggtc
    11101 agcaaatgtt gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt
    11161 ttactcctca tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg
    11221 gcggttctga actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc
    11281 ctaaagcaat caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt
    11341 tgcttttgat tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga
    11401 tggtatttga aaaaatgaat atcccatttg cagcggatat cgtcaatttt gtgattgtcc
    11461 ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc
    11521 gtttgaagga tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac
    11581 cgcaacgtgc tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt
    11641 ttgtcggtga caaattattt ggctatctgg caggttccct ttcgtataca gtattattga
    11701 tctggatcat tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg
    11761 ataaaggaat cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt
    11821 tcacgaactc cattggggta actgtattga ctggcctgct ttacctgttc atttatttca
    11881 gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt
    11941 tattttattt atgtggaata gatggagcta ttgaagaaag gagttgtgac aaaagtcttg
    12001 ttgcaactcc tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa
    12061 ccctctgtgc aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg
    12121 aaaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt
    12181 tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc
    12241 ttcagtcgta cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg
    12301 ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct
    12361 agtcttcttg tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac
    12421 acagcttgga tgaaggccat gattcctcat cattctatcg ctatcctggc aagtaaacga
    12481 gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa
    12541 gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa
    12601 ataaaaaaag aggatgttcc agaagttatc agcttcaaga aataagaaga agctacttct
    12661 ggaacgccgt gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
    12721 aaacaaaaat agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct
    12781 tttgaacacc gtttattaag aggttgtaaa atcagcgttc tgacctgagt attagagaga
    12841 caaatggaaa aatagttcct catattttgc cattttttga gctaataccg agggtcagct
    12901 gattgaacac cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa
    12961 aattttgtga aatggctttc cacatttttg tataaatttg gcttaatacc gagaaactgg
    13021 cttttgaata ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt
    13081 ttatgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat
    13141 ttttttgaga ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa
    13201 ctgaaaaata cgaatggcaa gtaagtgagt gtaggaatac caaatgtact tgctacgaaa
    13261 cttccggcaa ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagcgtt
    13321 ctacctaata cagttggatc aaatcctgct ttttcataag tttctttaaa tgcattacca
    13381 ggcagaataa cagataggaa ttgttcacca ataaaaatat ttactccgat acttgtcatc
    13441 aatgttgtaa aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg ttccatcacg
    13501 gttgagatca agccaaattc gatcaacaat ccacctaatg acaaagttag aataatcaat
    13561 gacacagtag gcatcatact ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg
    13621 tttccagtat ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt taaggaagtt
    13681 tgtgggtttt gaataaaaat cattcctact gctacagcga tattcaacaa aatcgttaaa
    13741 attgccggca cctttttcca tgcacacagt aacattaaaa ataaaggaat cagtgaccag
    13801 attgaaatag taaagtttgc ttctagaaca tcagtcactt cacgaataga tgaaagactt
    13861 gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccggg
    13921 acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct
    13981 gctagatttg ttgattcgga taatggagac atcttatctc caaaaacggc acctgaaaca
    14041 atagctccaa caaccagcgc tggatttaag cctagtgttg tcccaatacc gaaaaatgca
    14101 atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacac
    14161 acaacaaata cagaagggac aaaccactgg acactgatca atttgaaccc aatgaccatt
    14221 agagttggaa tgatccctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa
    14281 ataaagattg ggatgattcc tggtttgatg ccattgatga ttccttcgtg cagccaatct
    14341 gtcgggtatc tttttatcac tgcatacaac atgaccattc cgatcgctgc taagatcgtg
    14401 atattaggtg agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt
    14461 aatacaagtg ttgcttctaa aaaacttggt gttccttttt ttcccatgat aatctctcct
    14521 tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccgcaattca
    14581 tccgcgattc ctcctttata aaaataaaaa gacctgtttt aaactaagga cgaatgactc
    14641 cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacttt tattcaaatg
    14701 tatggacttt ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt
    14761 tctgaacaaa acacctaacc gctactgcgt gaaaagttga cttgtacgaa gtttaccttc
    14821 cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt
    14881 ataactttca cacacaagtt tattttttac tagaaagtag cgccctcttg tttttttctt
    14941 gctactattt cttgatgttc ttgcttgatt ctttctaata gctttggctc tttcaacaga
    15001 tcaagagctg tattggcgag tagttctgct gcaatcgcaa tagaagcaag gcctttgtca
    15061 cttttagcag cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc agagattgct
    15121 accgtaggct ggattgttgg tatgacttgg ctgacatttc ctacatcaga agaaccgaga
    15181 cttgttcttt gttccgtttc tatttcttca tctggaacac ctgcttcttt tgcatgctgg
    15241 aagaataagt catcaaaaga aggagtaacg atgacatcat caactgcatt ttggaacaat
    15301 ccaaactcaa aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt ttccactttt
    15361 tcatatactt tatctaatgt ttgccgattt cctgcacgta aatagaaacg tccagcagcg
    15421 taatctggaa tgacgttggc agctactcct ccgtctgtaa tgatcccatg gatattgaca
    15481 tcttttggca attgcaagcg tagagcgttg attccattaa acacttggat caatgcttcc
    15541 aaagcattta ctcctttttc aggtgctgct gctgcatggg aagcgacacc gtaaaatttg
    15601 atgtcgactg gatcattcgc taaagattct gtcgttttcc cataacgaaa agctggatgg
    15661 acgcataacg ctacatcgac atcctcaaaa aaaccttctc gaacaaagct gcctttagct
    15721 gatccgttct cgcccccttc ttcccctggg gtgccatata cacggatttc tccaccagtt
    15781 tcatcaatga attgcttcac tatgctggcg gctaatacgg agtaagtacc aaacaaatta
    15841 tgcccgcatg catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg
    15901 ggaccgggtt tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc agctacttct
    15961 ttttctaccc aaaacctttc tttttctaat tgtttaatca acgctgctga ggaataaaac
    16021 tcatagttac ttacttcagg atgttcgtgg atatctagag caagtgcttg atattctggt
    16081 aaacgatatt ggattagatc cttcaattgg tttgttgaga ttgttgttgt cataaaaaat
    16141 tcctcctatg ctagtcattt ttcttatttt ctcccgaatg tttcccatgc aggcacgctt
    16201 gatccttttg atgttttctc gattactttg actgtgtctt ctgtttgata agcgtctact
    16261 attttttgat acgttttatt gttttcatct tcttttcttg caacgatgat attcacataa
    16321 ggacgtgcat ggtcatccac tggttctaag aagatggcat cagaagcagg attaaatcca
    16381 gcatctacag ctacaccgct attgatcact gaaatgtcta catctgacaa agcacgtgct
    16441 gtttgggaag catctaattc agaaatgtct agattttttc tattctcggt aatatcagaa
    16501 acagtgggcg cttgtccttt tgccttatcc actttgatca atccagcagt ttgcaataac
    16561 agaagagctc tgccgccatt tgtaacatca ttagggattg ctacttttgc tccgtctttg
    16621 atatcagcta catccttcaa agtgttagaa taaattccca gcggtgcatt gacagtattt
    16681 ccgatcgata ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt
    16741 tggaaagaat tcaaatcaat cgatccatct gctaaagcca catttggttg attgtaatct
    16801 gtaaatttca cgtattccaa attaatgttc tcctttttca aacggtcttt cacgttgtcc
    16861 catacatctg tatcttctcc aataatccct agttttacga tagtttcttt tttgtcagtt
    16921 gtatctgctt ttccttggct acaccctgcc aaccaaataa ttgctacaaa tgtaatgact
    16981 acaaaacgaa taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat
    17041 cagttgatta ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac
    17101 aatcgttaca tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc
    17161 gcctgcacct actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc
    17221 cgagacccga atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat
    17281 tggactggtt cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc
    17341 gttctcgatt tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc
    17401 tttcgcacca atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa
    17461 gataatgaaa gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag
    17521 acggttagct agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc
    17581 aatcatcccg ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc
    17641 gacaaattca tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat
    17701 tttatcttcc tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat
    17761 ttatttttac accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc
    17821 cagctaaagt aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat
    17881 ataaaatatt cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg
    17941 tctgatttcc aatataactt aattccacca gccatttatt ggcgatctga tcggcaaaag
    18001 cagaatgggc aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat
    18061 ctttggtcaa ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga
    18121 ttcttccatc ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat
    18181 gagtgatcag gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga
    18241 tctgaagggt cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag
    18301 gttcattcgc caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt
    18361 taggataagc agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt
    18421 gacgacgttc ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa
    18481 tagttcggct ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt
    18541 ttcgcaattc tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg
    18601 ctgttggttt ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt
    18661 aaccgacgat tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct
    18721 gtatctcttt ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa
    18781 ttcctcctta tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg
    18841 cttttttgcg tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt
    18901 acgatcatac tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg
    18961 atgaattgct cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag
    19021 ctctctgtca aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa
    19081 aaagtccttt tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct
    19141 tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt
    19201 ttaacggtcg aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt
    19261 ttcagctttt cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac
    19321 ttactttcct tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa
    19381 ctgaaaagtc aagtacgaaa ttcaaattat tttttctata tttatttttc tgttttttta
    19441 tttaacattt caataacagt tcgaagatct tttccttccg caagaagttt caatttttcc
    19501 ttttcttcga cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat
    19561 cctgctcgtg aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta
    19621 gcttctaatc ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc
    19681 gacacttcgt ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag
    19741 atatctgttg ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct
    19801 tttaagtctg ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc
    19861 tgttgataaa aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct
    19921 gctgctttca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg
    19981 atttcttttg ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac
    20041 gttttttctg tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca
    20101 ttaatccttt ccaaaacaat acacatagtt tagcacgact ggtccaaaaa gaacagaagc
    20161 aaagaaccag ccttctcgtt tataagagtt cttggaatcg acggatatag attttcttca
    20221 cagctgtcac aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc
    20281 caggaagtgg catcaagcct aattcagcac caaaacttgt aaaaggaagg acagaaccga
    20341 caataattcc taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt tggataaatg
    20401 gtactttcgg tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa
    20461 accatcctgc atggaataat gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga
    20521 aacttccgcc cactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat
    20581 caaatatcga acttgttgga ccaaagtaaa ccatgaattc cccaatacta gatgcattcc
    20641 atttttttgg ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg
    20701 aaacatcata gatcagattc aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca
    20761 ggaacgtact tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct
    20821 tgatatattt catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa
    20881 tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg
    20941 tatctacaga aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc
    21001 ctaaaaatcc gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct
    21061 gaggattcaa ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac
    21121 tcatttccga gattttctca ccggtaacca attcattgat ctctagacct acttgacggc
    21181 acactgaacg tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt
    21241 ctttcagagc tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagcaagat
    21301 aaccaatcaa aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat
    21361 tggtcttttg tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg
    21421 caagtacggc ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa
    21481 atttagaaat gttcagcatt tcttcaatcg cgccttttgt gatcatttgt gttttaccag
    21541 ctggatcagc aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca
    21601 ctttttgata attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca
    21661 catctaacaa atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa
    21721 cacgatcatc ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg
    21781 tcaatgtccc cgttttatcc gtacataaca catcgattgc accaaagttc tggatggcat
    21841 tcaaattttt gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg
    21901 tcgtaacgat cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa
    21961 gaaaagcttc tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca
    22021 tcaatgacat aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg
    22081 attgaattgg tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa
    22141 cagaaacaat gattccttcc gcacttccac tgatgacatt acttcccata aagacaagat
    22201 tttcgtaact tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg
    22261 gatagctttc ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac
    22321 gcatatccgc tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt
    22381 cttccatagg aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa
    22441 gtgatttcag tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc
    22501 cgctgacgat aaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag
    22561 ctgctaatac atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa
    22621 atggcgtgat gtatgctttc aatatttcta caatgaatgg tgtttttttg ccatatgaaa
    22681 tcgcattttc accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg
    22741 ctgatgcatg gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga
    22801 ttttttgtgt atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct
    22861 gttctcgctg cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg
    22921 gctgagaaca gatagataat agccctggcc tttcctctag atccttgttt ttcgaatgac
    22981 tgtcatccat ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat
    23041 ttttcgaata gttatttttc caattagacc acctcctttt tatttttcac cgatggggat
    23101 ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta
    23161 aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata
    23221 cctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc
    23281 gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct
    23341 tattcggttt tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca
    23401 tgtttttcat acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt
    23461 gtctcatttt ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg
    23521 aaagaacact tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca
    23581 tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc
    23641 ctgaattagc aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga
    23701 tcgattacat gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg
    23761 atatcgtccg taagatggtt ggagcaacaa aagctgccga tgctgttcca ggtacgatcc
    23821 gaggagacta cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg
    23881 ctgcggtaaa agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa
    23941 aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc
    24001 agcagttttt tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg
    24061 aagaattctt ggtaatcttt cgtcttttct tcccctaaag cagcagaaat caatctttga
    24121 taataatctg ccatcaatgt ttcatagatc actgtacttc tttctttatg tgctaagctc
    24181 cgtttcatat cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac
    24241 gaagtcaggt tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat
    24301 cgtcgcattc tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc
    24361 caaggaaaat cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag
    24421 tattcttcaa tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct
    24481 gttttttcga ccatgtgaag attcaaaacc aaataataca gatcttctgt tttcttaaaa
    24541 tgcctacggt tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct
    24601 agcattcgtc tttttttctt taactttatt ggttcatcca gctggtttcg catccattca
    24661 tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt
    24721 tttttcactg aatttgtctc aatcccgact agagaagtct taaaacagat tccttccgct
    24781 aattgggact gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac
    24841 ataatagttc tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa
    24901 ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc aaccttcaag
    24961 cattagaatg ttgtctaact actgaaggtt ggtttgattg tttattgatt atttttttgc
    25021 tgttcaacgt ctgttcttgt tactggatat aagttggttg ttgttttatt accatttttt
    25081 gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt acgttccgct
    25141 tcatttttat agtcatattt tttcggatca atcgtctcaa atccgtttgg cacataaaac
    25201 cgcaagagat tttcctgatt cagcttatct gaaagactta acgcagtctt gactttttcc
    25261 tgatctttgg agatttcctc atcgattcct gctgctttcg tatcgatgat ttctcctgtc
    25321 tgattattgt aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt
    25381 cgaaaagcaa ctacttgatc atgctgagaa gataataagt ctgtaccaaa atgaagataa
    25441 tctttatcat cgattcccaa tagatgtagt aatgtcggca aaacatcgat ttctccaccg
    25501 tattcatgat tgactgtccc tttagtataa ccaggcatat ggatcatcaa tggcactcgt
    25561 tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat ctttaccaag agcgcttgct
    25621 aaatctttat tatctgtatt cgagatccca aaatgatccc cgtaaatcac aaagattgaa
    25681 ttttggtaaa taccagattt tttcaaatac gtaaaaaatt gttccaatga ttgatccaga
    25741 taatgtgccg ttcgaacata attatctact gtgctattgc cagtattcaa agatggaaag
    25801 tcgaaattct tgtcatccgt ataataaggc gtgtggttgg ttacggaaag aaatttcgtg
    25861 taaaacggct gttgtagatg ctcaagatat tgggcagatt ctctgaataa atctttatct
    25921 aatatcccat aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg
    25981 tcataaccta agtttttata tacatgatct ctattccaaa aactagctac attcccatgg
    26041 aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca
    26101 ttgtctgacc caagttctgt gaataatgag ccttgaggta aaccaaatgt gccagtttcc
    26161 aacatatttt cagcatcact tgtctttccc tgtcctactt catgaaagaa attgtcaaag
    26221 ctgatcgttg cttgattctg gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct
    26281 accttcatat taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat cacatttttc
    26341 cctttagcga tcccaaatgt ttcgggattt ggctctgcat aatgtttctt tgtataatct
    26401 aaaacttcat caatcccatt actgcttgca tgcgcgcgaa cactgctagt cattcctgtt
    26461 ttgattccgt cataaatagt atacgcatcg attcctagat atttgacaat atatgaacgg
    26521 tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa
    26581 aaaataagcc ctgaaagaca ggtaacagcg atagccatcg gtttgccgac agggttgctt
    26641 ttgataggta ttttcttgac cttcaaccaa accagtagcc caatgaacac agcgatatcc
    26701 agccagtaga tgacatcatg gggcttcatc aaagaaaagg agcttcctga caatccttgc
    26761 gacactttcg aaaagcctaa tacagatttg actgtaatga aatctgtgaa ttcccgataa
    26821 aaaataatat tcaagtacaa caaaagcgta tttaaaatat caatgatcaa aagaatagct
    26881 aacgctggct ttggcttttt aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca
    26941 attgggttga tccacaagat aaagtactgg atcgtacctt ccacacctaa tgaaaaatct
    27001 gcatagtagg caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa aacgattgct
    27061 aaacgatttt ggcacaattt tttaagttgt gtcacgtata tcacttctct ctattcacat
    27121 tttttcttat tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt
    27181 tttttattct ttctaaagat tattccgtcc acttttcaca gcataaaacc gctgcgatgt
    27241 aagcgattaa actaatgaag agataaaagt cttgtggtga ccgcttcact ccacggtatt
    27301 caaaagccag tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca
    27361 gctccttatt tcttgatgca ggacagcttt ttcacaactt cttccttaaa cggtgttctc
    27421 ctgccaactc ctcggccaca agtcacaata tttataaaac atgagaagct gttttttgaa
    27481 attgttcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    27541 aatcagctga ccctcggtat tagattatgt caggacgcta attttacaac ctcttggtaa
    27601 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atataaggag
    27661 ctattttttg aaattgctcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga
    27721 tacacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgagga
    27781 actattttgc cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctctgt
    27841 ttttcattgt ccataggatt gatattgtcc ttgtgatgac tcattcgtaa taattgaggc
    27901 ttgattttct tcacttcccg ttatcgtttg tccactgcca gaatctgaag gacttgttcc
    27961 tgtcgactcg tttttttgag aagagacagt tgttttcttg acttgttcat ttgtcttttt
    28021 ccattccgct aaacgatcgg cgaccacttt tttgtctact ttttcagaat aagcggtgat
    28081 cacttttcca tttttcaaac gggctacttt gtcgccatca tgagtcaaaa gcaaaacggt
    28141 caaatgcttg tctttaaatt gatattgtct gagagaaatc gcactaagta cgtcatcgat
    28201 attaataaat tgattttctt cttttccttt tggcaaataa atgtccaatt cgcctttttc
    28261 gtaattggca gttattttat actccgccca ccaataagtt gagaattctt ttttgaaatt
    28321 atctactgat tctttgcttg ctacgatttt ttctgtttct ttctgagcaa gatctgctgt
    28381 catttttcct gaaaatgctg cggataaaat aagcagtaag aaaaaaccga ataaccaaaa
    28441 gagaagaaac ggtctttttt tcctcatgct ttcctccact cctatttaat tttgactatt
    28501 ctattatata caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc gtaaataatt
    28561 gaacttcttt ttcacaaaat ggtaagaatg gagaatttca cgcatttgct ctcaactatt
    28621 ctttcttaag cctcaaatcc gtttttatct gatacgtctt tgaaccaatg acccgatttt
    28681 ttgattgttc tttttccatc ttgtgctaag tctactgaaa taaagccata tcgattctta
    28741 tatgcattgg tccacgacca gttatccata catgtccaca tatgatagcc taaacaattg
    28801 gcaccttctt gaatggcttg atggacatat ttcagatgag attggacaaa ttcgatccga
    28861 taatcatcgt ggatcatgcc atcttctttg ataaatcgtg cttcgttttc tacacccata
    28921 ccgttttcag aaataaagca gtcgatattt ccataatgat ctcgtacatt gatcaaaata
    28981 tcatagattc cttgttcata gatctcccat ccgcgataag gattcatttt acgaccaggc
    29041 atttcataat tgtcgaaata atcctcaggc attggaccgt ttttagtatc gattggtgtt
    29101 tcttttgctt ttacccggcg tggctggtaa taattgatac ctaaaagatc tactgtattt
    29161 tctttgataa ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag ttcttttact
    29221 atctcaatca attcacttgg aaactcgcct tttacagctg gatcaaggaa tgaacgattg
    29281 aagaacgcat ctgctatttg cgctgcttta acatcttcag ggtttgattc gtctcttgga
    29341 tagcttggtg tcagattcaa gatgatacca ataacaccac cttgctccat ttcatggtaa
    29401 gatttgatgg ctaacgctga agccaacgct tcatgatagc caacttggat cgcttttttc
    29461 atatctgact cttctggata atgccatcga tataaataac ctgcttcgac tggaacgatt
    29521 ggttcattat gagtaaacca tttttttact ttcgaaccaa acaattcaaa acaacttttg
    29581 gcatattttt cataggcttc aaccgtttct cggttcaacc atccgccttt ttcttgcaga
    29641 tacatcggca tatcgaaatg atacagattc acaaagggtt cgataccgtt ttcaatcaac
    29701 tcatcaaaat aacgatgata gaaacgaacg gcttcttcgt tgattgttcc agattcatca
    29761 ggtatcaaac ggctccattg gatagatgtt cgaaatgaat tatgcccagt ctgtttcatc
    29821 aactgtacat cctcttgata cttcgtataa acttgcgagg ttttttcagg tcctacttga
    29881 tcaaagaact tttgcggttc ttttttgtac caataatcaa aaatgttctc tccttttcca
    29941 tcgcctgaaa tccttccttc tgtttgtggg ccacttgctg cagaacccca ccaaaaattt
    30001 gctggaaatt gatatttttt catcaaagca cctctttttc ttttttagtt cgttttagtt
    30061 gattccatgt atgatatttt ccatcacaat cactttttca ttttcttcct cttctttttt
    30121 gattcgtttt aacagctgtt ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat
    30181 cgtgattttc ggaaggatac tcagatcgtt tttatctaaa taatcatagt gactgaacgc
    30241 aatatccttc ccgattctga caccttcttg attgaaacat tcgatggcac caatcgacat
    30301 attgtaattg ctgacaaaaa tagcagttgc ccctttttct aataatgctt ttgccccgta
    30361 atagccgctt tttctagaat agtctcctgt aaagacctct aattggatcg atggatcatt
    30421 ttggaccgct tctttggctc ctgctaaacg ttctcttgct acgtagtctg tttgagtggc
    30481 agcaataaat ccgatttttt tatgtccttg ttcaccatga tgagcgatca cttttgctac
    30541 actttctcga tcattcacta atattgtatc aatacctgct ggtccttctg gacaattcaa
    30601 tgcgataact ggaatactca tttttgtgag catttcaaat ccagaccact cttcttcgga
    30661 taagaagaca atcagcccat cgatcacatg cttcatcagg tattcaattt tttgttcaat
    30721 tttttctggc tgatcggcaa acccgcttaa taataagcta tacccatttt cttcagctac
    30781 tctttcgatc cctgagacaa cttccgaccc aaaacggctg gaaatcccgt ttatcaaaag
    30841 tccaatagag aagcttcgat tattcttcaa ccctttagca atgatgtttt ctttatagtc
    30901 caacgctgaa atcgcttctg ctattttttt catgttcttt tccttcagtt gctgaccatt
    30961 caagtaacgg gaaactgttc ccactgaaac accagccatc ttagccacat cacggatcga
    31021 tgccatacat tctcactcct gccagttaca tttgatttct ctttcttgtt gttcgattat
    31081 gatgaccgca ttctttgcat agtgttgctt gtcatcaata tccatataac taaagtcttc
    31141 agtagagcaa cttcctctca ctattaatta tacttaaaca agtatttgaa ccggttcatt
    31201 tttaatatag cattttttta aaagattgca acactttttt aaaacgcttt ctgaaagtgg
    31261 cggatttttt gcctttttaa aattttattc taaaatttac tctatttgtg ggtgttttgt
    31321 gcgtttttct cttccatccg tttgaagttt tacattccaa tggtataatt tctttatcct
    31381 atcaaatggg ggaagtattt tgaagaaatt aatcgtacta tttattttgt ctgcgggact
    31441 tttgacattg actggctgtg atttgtttgg cggctctgac caatcctcgg acgaaacaac
    31501 cgattcttct gtcttaaaag cggaaaaagc agataccacc aacagtgtat atacgctaaa
    31561 agtacgtaaa gatatggatt tatccgcggg acagttgaca tttacagata aaaaaatgga
    31621 atggacacgc acgtatcaga cacaaggaac tgattcttct gaatcatctg aagaaaaaac
    31681 agtgctgaca gatatcaaga tcactaccaa agatgacgaa tatattattt caggaaaaga
    31741 aaataaaaaa gaaacaacag tcatatttac aaaaatcggt aattaccgtt tgaaggatga
    31801 ggatggcaac atctacagtc tatagtgccg tctgttggat cttcttttgt ttctcccctt
    31861 cttttctgct atagtaaaga agcaagatga ttgtaaagga gacgaaatgg atgatcgata
    31921 gtcctgatta ttgtacatgt aataacaaca aaaatatcca tacagaattt aatgaccaag
    31981 gaagcttgag catatgcgac aactgtcaca aactagtcaa agattcattt aaaacatttg
    32041 aatcttatcc tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat ccaagcaaaa
    32101 ctcactatgg ctgattttta tgaacttcaa actcagcttc ttcctttgat caagcttcat
    32161 cagtaaaaca aacagtggat catttcagtt tacaaatcaa agagtaaaga tttatttatt
    32221 tcaaatattt gaatataaaa taaccaaacg atagaaaggc ttattctaca tcacgtagta
    32281 atttttcttt cccgtcgttt ggttttttat ttttagccta attcttgacc ttttgcatgc
    32341 tggttatccg taaaatattt tccagcaggc tttccttcca attcagctaa tttttcttgg
    32401 atctgacgga tcaccttttt tgcatcttct ggattttcta caaaatcgaa tttatcccca
    32461 tccacttgta ttttaggaca tacatcaaaa tcttcatacc attgattata gcgggtgttc
    32521 aacgtttcat aataggtata taacgtctca tcgctttcta gctgttcata gtcacgccca
    32581 cgttttttga tacgttctag catcgtgtcc aaagaaactt tgatatggac catcaaatca
    32641 ggccgttttt ttggagcaac atcatctaac tcgtttagca tcgtatctaa taagctatcg
    32701 tactgctgta cttcgatatc cgttacccga cctaaatcag cgtttagatg gaacaacagg
    32761 ctatcttcat agatggatcg gtctagcaca ttgtcatcgt tcactaaagc atttttcatt
    32821 gctaaaaatc gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg ctcaggattt
    32881 gaataaaaga gcggcaatac ttcgttatct tcgacatttt cataaaatgg tttagaatca
    32941 atctcttttg caagcatatc cgtcaaactg ctcttacctg caccaatcgt tcctgaaatc
    33001 aaaatcactg acatacaacc cttcctcttc tataagtact cgcctacgta agtttcaaca
    33061 ggcaatgttt caaaatcata ttcacgtaca gcttggtctg attttaaata cgtatggaga
    33121 ctttgtgtaa tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat catcggtttt
    33181 ttcaacatag ttgccacacc taattcgtaa gccgttcctg gatcgattgt ttgatgctca
    33241 aagtcaagga tagcaacaac aaattctgca tttgtcagtt cttccatatc tttttcgtat
    33301 acttcctgcg cccatccagc gctgaatagt tcataattgc tttcttggtg atgacgtgga
    33361 gagtagaaac ttgtgactgt tttgttttcc tccaacgctt tttcgatacg acttactcga
    33421 tcgatttgct cctctgaaaa aaatggtcct gctaaatata tttgactcat tattttgcct
    33481 ccttcaataa taactgattt ttaatagata ctgctcgtaa ttcattgacc agttccaatg
    33541 gattgttgga aaagattact tgatccaacc attttttatc cataaaatcc tcttttgcaa
    33601 aagtttctaa taattgttgc aagccgctgt aataaccgcc aatattcaat attccgattg
    33661 gcttgtcggg cgtcagaccg attgctatcc aagaaagaag ttgtgagaat tcctccaatg
    33721 tacctagacc tccagggaaa acgaggacag catctccctt ttcaaataat aatacctttc
    33781 tttcatcgat cgtttctgta ggaataaatg tagtaacatc ttttccaggc aattcagttt
    33841 caaaaagccc attcggatag atacctgtga cttttccgtt attttccagt actgttttac
    33901 taatcactcc cataatcccg gagtatgaac caccgtaaac cagttcgatt tcttcttgtg
    33961 ccatgtattt tcctaacgtt tgcgcaataa atttgtaact ttctttatta ccaaaacggg
    34021 aaccgcaaaa aacagtaagt ttcataaaaa aacactccct aactcaaaag tctatcttca
    34081 ttctacacgt gtttttttaa aataaaagac tagattttgt agttttttag tggtatat
//
LOCUS       DAXWLH010000008        33098 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXWLH010000008 DAXWLH010000000
VERSION     DAXWLH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.008"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            39..896
                     /locus_tag="ACGE36_000278"
     CDS             39..896
                     /locus_tag="ACGE36_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFF8382707.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1011..1931
                     /gene="pstC"
                     /locus_tag="ACGE36_000279"
     CDS             1011..1931
                     /gene="pstC"
                     /locus_tag="ACGE36_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFF8382708.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1931..2815
                     /gene="pstA"
                     /locus_tag="ACGE36_000280"
     CDS             1931..2815
                     /gene="pstA"
                     /locus_tag="ACGE36_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFF8382709.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2830..3636
                     /gene="pstB"
                     /locus_tag="ACGE36_000281"
     CDS             2830..3636
                     /gene="pstB"
                     /locus_tag="ACGE36_000281"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8382710.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3652..4410
                     /gene="pstB"
                     /locus_tag="ACGE36_000282"
     CDS             3652..4410
                     /gene="pstB"
                     /locus_tag="ACGE36_000282"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8382711.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4423..5100
                     /gene="phoU"
                     /locus_tag="ACGE36_000283"
     CDS             4423..5100
                     /gene="phoU"
                     /locus_tag="ACGE36_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFF8382712.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5677..6369
                     /locus_tag="ACGE36_000284"
     CDS             5677..6369
                     /locus_tag="ACGE36_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8382713.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6366..7133
                     /locus_tag="ACGE36_000285"
     CDS             6366..7133
                     /locus_tag="ACGE36_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8382714.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7233..8801
                     /gene="liaX"
                     /locus_tag="ACGE36_000286"
     CDS             7233..8801
                     /gene="liaX"
                     /locus_tag="ACGE36_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFF8382715.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9098..9418
                     /locus_tag="ACGE36_000287"
     CDS             9098..9418
                     /locus_tag="ACGE36_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFF8382716.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9418..9768
                     /locus_tag="ACGE36_000288"
     CDS             9418..9768
                     /locus_tag="ACGE36_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFF8382717.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9935..10873
                     /gene="hprK"
                     /locus_tag="ACGE36_000289"
     CDS             9935..10873
                     /gene="hprK"
                     /locus_tag="ACGE36_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFF8382718.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10888..11718
                     /gene="lgt"
                     /locus_tag="ACGE36_000290"
     CDS             10888..11718
                     /gene="lgt"
                     /locus_tag="ACGE36_000290"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFF8382719.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11762..12787
                     /locus_tag="ACGE36_000291"
     CDS             11762..12787
                     /locus_tag="ACGE36_000291"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8382720.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSRAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12802..13740
                     /gene="galU"
                     /locus_tag="ACGE36_000292"
     CDS             12802..13740
                     /gene="galU"
                     /locus_tag="ACGE36_000292"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8382721.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13865..14305
                     /locus_tag="ACGE36_000293"
     CDS             13865..14305
                     /locus_tag="ACGE36_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFF8382722.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14308..14910
                     /locus_tag="ACGE36_000294"
     CDS             14308..14910
                     /locus_tag="ACGE36_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8382723.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14969..16075)
                     /locus_tag="ACGE36_000295"
     CDS             complement(14969..16075)
                     /locus_tag="ACGE36_000295"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFF8382724.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16285..17286
                     /gene="ccpA"
                     /locus_tag="ACGE36_000296"
     CDS             16285..17286
                     /gene="ccpA"
                     /locus_tag="ACGE36_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFF8382725.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17623..19968)
                     /locus_tag="ACGE36_000297"
     CDS             complement(17623..19968)
                     /locus_tag="ACGE36_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFF8382726.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20247..21149
                     /locus_tag="ACGE36_000298"
     CDS             20247..21149
                     /locus_tag="ACGE36_000298"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFF8382727.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21362..23137
                     /locus_tag="ACGE36_000299"
     CDS             21362..23137
                     /locus_tag="ACGE36_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFF8382728.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23203..24789)
                     /locus_tag="ACGE36_000300"
     CDS             complement(23203..24789)
                     /locus_tag="ACGE36_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFF8382729.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            25040..25264
                     /locus_tag="ACGE36_000301"
     CDS             25040..25264
                     /locus_tag="ACGE36_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFF8382730.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25405..27156
                     /locus_tag="ACGE36_000302"
     CDS             25405..27156
                     /locus_tag="ACGE36_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8382731.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27156..28940
                     /locus_tag="ACGE36_000303"
     CDS             27156..28940
                     /locus_tag="ACGE36_000303"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382732.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29266..30117
                     /gene="licT"
                     /locus_tag="ACGE36_000304"
     CDS             29266..30117
                     /gene="licT"
                     /locus_tag="ACGE36_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8382733.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30213..32093
                     /locus_tag="ACGE36_000305"
     CDS             30213..32093
                     /locus_tag="ACGE36_000305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8382734.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(32133..33035)
                     /locus_tag="ACGE36_000306"
     CDS             complement(32133..33035)
                     /locus_tag="ACGE36_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8382735.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        10510 a         5877 c         7024 g         9687 t
ORIGIN      
        1 ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt aaattattct
       61 taccaataat aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag
      121 aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca gttgctgaaa
      181 cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg
      241 gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg tttgcggaag
      301 aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt gttgggatca
      361 ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac ctgaaaaaac
      421 tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag ccagtagtta
      481 tattgaaccg cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg
      541 gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgcgc cagatcgttg
      601 ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat gatgtagata
      661 cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac aaatggacga
      721 tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca aaagaatttt
      781 tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta ggttatatcc
      841 ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt
      901 tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc
      961 aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg
     1021 tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca
     1081 tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtag
     1141 ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg
     1201 gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta ggggcattgc
     1261 ctatgatcac aggttcgttt attgtgacct ttttatctgc tatcgttgcc acgccatttg
     1321 ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg aagatcttac
     1381 agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt atcggtttat
     1441 cagttatcgt gccttttgtc agaagtattt ttggcgggac tgggtttggt atcttagctg
     1501 gtacatttgt gttgttcgtt atgatcttgc cgactatcac aacgatgact gttgatgcat
     1561 taaaggcagt gccacggcac tatcgggaag cttcattagc gcttggtgcc acaagatggc
     1621 aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt
     1681 ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc ggtaatgcag
     1741 cattgatgcc aacaggtctt actactcctg cttctacatt gacgtccatc ttaacaatgg
     1801 gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga
     1861 tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag
     1921 agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt tatatatagt
     1981 ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag ttcggggaat
     2041 accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag aaggcggtgg
     2101 aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga tcatcagttt
     2161 tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa actggctaac
     2221 agatgtgatt cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt
     2281 attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta tttctggtgc
     2341 attggctttg actgtattca acttgcctct tttgactaga acagttgagg agtctttaca
     2401 agcggtccat tacacacaac gagaagcagg attggcctta ggtttgtcac gctgggaaac
     2461 agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg tgatcctaag
     2521 ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac aaagtgcacc
     2581 tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca gcattttccg
     2641 tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca cgatgcctga
     2701 tggtgtttct gtttcggctg gcgcttctgc cgtgttgatt attgctgttt tactgttcaa
     2761 cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga
     2821 agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat gccaaaagag
     2881 aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa tgaagcaatc
     2941 aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg tccttccggc
     3001 tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat cgctaatacg
     3061 aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca agtggatgtt
     3121 tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg
     3181 atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta
     3241 gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt aaaagacagc
     3301 ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg tattgctcgt
     3361 gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc tttggaccct
     3421 atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta tacgatcatc
     3481 atcgtgacgc ataacatgca acaagcagct cgtatcagcg actatactgc ctttttctat
     3541 ttaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc aaaaattcag
     3601 gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa
     3661 gagattattt catctaaaga tcttcatctg tattatggaa agaaagaagc actaaaaggg
     3721 atcgatctaa gcttcaatca aggggaaatc actgcgatga tcggtccttc tggttgcgga
     3781 aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc
     3841 accggaagtg ttttatataa agacaaagac atctatggtc caaaaacaga tgttgttgaa
     3901 ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt ttctatctat
     3961 gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa
     4021 gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga caaattgcat
     4081 aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg
     4141 gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga tcctgtttct
     4201 actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg
     4261 acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt
     4321 cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa acaagagaca
     4381 gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt
     4441 gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc
     4501 gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc ccaagaagtt
     4561 atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa aagttttgaa
     4621 atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac ggtcatgaaa
     4681 gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa atccacgatt
     4741 cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat
     4801 tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga cgaaaaagac
     4861 gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat
     4921 gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac agactacttg
     4981 aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg tgaatggatc
     5041 gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga gattttctaa
     5101 actgaagctg tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga
     5161 aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaaatagctc ctcatatttt
     5221 tcgtttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgggaaag
     5281 ggtttgtgac aaaacatgtc acaaaccctt ttcatcattt catagaaagg ataaagaatg
     5341 caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc atgggttatt
     5401 cagcatcaag ccgctttata tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa
     5461 gaggatcatc aattgattac aggaaaactg atgaacacgg atcaaaaagg agaatcgatt
     5521 tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa agcgagacaa
     5581 atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa aaatgatggc
     5641 tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg tggaaaacag
     5701 ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc
     5761 atctttacta aaacagaagg gatttctcta ttaaatatat ctatcctgtt cgtcgttata
     5821 gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt
     5881 tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatcat
     5941 tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct
     6001 gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca
     6061 gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta
     6121 tggctttcag gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg
     6181 ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtcg
     6241 ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc
     6301 ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt aaggaggctg
     6361 aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg
     6421 gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg atctttttcc
     6481 tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt
     6541 tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag
     6601 cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt
     6661 ttttagcaat ccaaggtttt acatcggtag aactagaaga attatctggc ttagataaga
     6721 ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg
     6781 ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc
     6841 cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg
     6901 cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct
     6961 ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag
     7021 aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct ttaacctgcg
     7081 tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac
     7141 aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct
     7201 tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt
     7261 tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa
     7321 ctgaaaaaga cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct agtcataaag
     7381 aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg
     7441 agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa aagatcttag
     7501 atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat
     7561 tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa
     7621 aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg ttagaagcag
     7681 aaatcaaaga attagaagct agtggcgata cactgttaga agaacaattg aaattagaag
     7741 cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata
     7801 agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg
     7861 aaaaaatgtc agaagctgga tcacagttag gcaaattctt gaagaaaaca ttccaaactg
     7921 tttcagaaac agtgaatgac aacatggaat ggaaagatgt cagcttacgt gtccctggga
     7981 ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg
     8041 atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag
     8101 ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg
     8161 aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg
     8221 tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac
     8281 ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta
     8341 cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa
     8401 acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg
     8461 gtacagtgac atttggtgcg acagcagaaa acctttctgt gtctttggta aatggtgatg
     8521 tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa
     8581 atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg
     8641 gaagtatcaa cagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa
     8701 accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa
     8761 cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg
     8821 ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg
     8881 tgccttattg ccgatagtct atctcttgaa caccgtttac caagagatta tgaacctgcc
     8941 aattatcgcc gagtcacaag gaataatttc agaaaaagct cgtcacgttt tatgaatatg
     9001 gtaatttgtg accgagaatt ttgcagaaag acaccgatta agaagaaggt tgtcacaacg
     9061 attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa
     9121 atctaataaa aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat
     9181 tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc
     9241 aattatttta tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa
     9301 ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca
     9361 aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg
     9421 tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt
     9481 cctgcgatgg tccatgtgag cagtatttgg acggccgttc ttgccagctt cgtattgtct
     9541 gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc
     9601 acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt
     9661 gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg
     9721 atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg
     9781 ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt
     9841 aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga
     9901 tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca
     9961 attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt
    10021 catcgtaact ggggacatct cacgcccagg actagagttg actggttatt ttaattatta
    10081 ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat
    10141 cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat
    10201 tatttcaaga ggattaccag ctccagaaga aatgatccag gcagcaaata aaaatgggat
    10261 agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt
    10321 agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt
    10381 aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat
    10441 caaacgcggc catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt
    10501 aacggtgatc ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg
    10561 gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca
    10621 gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga
    10681 agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac
    10741 aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat
    10801 gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc
    10861 aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag
    10921 cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgct
    10981 atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat
    11041 tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta tgttttattt
    11101 gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta
    11161 gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat
    11221 tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca
    11281 atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc
    11341 ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc
    11401 tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt
    11461 ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta
    11521 atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt
    11581 ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt
    11641 ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa
    11701 aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa
    11761 aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt
    11821 cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga
    11881 aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac ccgattccat
    11941 ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat
    12001 tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc
    12061 agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat
    12121 cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag
    12181 ccgtgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa gtacagatgc
    12241 aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa
    12301 tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc
    12361 tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg
    12421 attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgctga cctttatcgg
    12481 attaagcgga ataggcgatc tagtcgttac ttgcacaagt gttcattcta ggaactggcg
    12541 cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca atatgggaat
    12601 ggtcgttgaa ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa tgatggaaat
    12661 cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag aaatcaaaac
    12721 agccgcaaaa gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct cattaacaat
    12781 tttttgaagg agcgaatgta tatgaaagtt aaaaaagcag ttattccagc agcaggatta
    12841 ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc aattgttgat
    12901 aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggtatcga agacatcttg
    12961 atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa tattgaatta
    13021 gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga agaaacgaca
    13081 gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca tgccgttctt
    13141 caagcaagag cctttattgg aaatgaacca tttgtcgtta tgctaggcga tgatataatg
    13201 gaagacgaag taccattgac acagcaatta atcgatgatt acgaacgtac tcatgcttct
    13261 acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa aatacggaat tatcaatcca
    13321 ggagaagtat tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa accagatcca
    13381 agcaaagcac caagtgattt agctattatt ggacgttact tattgactcc agaaattttt
    13441 gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa tccaactaac tgacgcaatc
    13501 gatacattga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa acgttacgat
    13561 gtcggtgaca aatttggttt tatgaaaaca agtattgaat atggtctaac acatccagaa
    13621 gtaggtgcac ctttacgtga gtacatcctg tctttaggta aaacattaga agcagaagga
    13681 agcaagatcg atcataatcc aaaaggaata acagaaatca aagaagaaaa gaaaaaataa
    13741 cccttttttc taacgagctt cagctgctaa ttgaataaaa agtagttgaa gctcgttttt
    13801 atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag aagggagttt
    13861 gatcatgtca ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg cgatacttgt
    13921 cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag tagatcagac
    13981 tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag
    14041 acaagtagaa ggcttactgg tcaaaagtaa tgaactgctc ttggatatca atggaaaagt
    14101 cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg tatcagaact
    14161 taatcattct agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa
    14221 agcgacggtt gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca
    14281 tactgaaacg acaggaggaa aataaaaatg gctaaaaaag gtggattttt attaggtgca
    14341 gtagtcggag gcacagccgc agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa
    14401 gaacttcggg atgaattagc tgcccaagcg gatgatttga tggatatggc ttcagattac
    14461 acagatatgg cgaaagaaaa atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa
    14521 gatctttctc aacaagcagg tgatctagca ggtagtgtca aacaaaaagt aagtgaatca
    14581 gcagatgaag caggaagtga atctgacgat atcttgttaa atcttaaaga acaatcagct
    14641 gacttatcta atcgtttcaa aaaaacggca gaagatatga aaaaacctgc agatgaatta
    14701 ggaagtattg ctaaagatac ttcagaagat atcatgattg atgtaaaaga cgctgctggt
    14761 aaagcaggag atactgtcaa tgaaggaatg tcagaagcaa aaccagttgc agatgactca
    14821 aaagaaatga tgaaaatggc aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat
    14881 acaatgacag attcatctac aaatcactaa aatcacaaat ttatagaata aaaaggtggg
    14941 agcggactag ctctcacctt ttttactctt atttttctaa aacaagtaat tcttttggtg
    15001 tagtcgtgaa tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc
    15061 ctactttttc aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta
    15121 ctaagtcatt tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac
    15181 ctaaacggtg attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt
    15241 gatcaagttc acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt
    15301 caagagtgat gttgtagatt ttttcttgga agtcggttgg tttttgataa gcaattgtac
    15361 gagtagcatc actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat
    15421 tcggtgaaat cttcgtattt cctggtgtac catgagggct tgcagcattt gctcctgcaa
    15481 ggactaatgt gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcaattt
    15541 ctgcaatgat cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg
    15601 caacatcggc ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca
    15661 gttgtagacg ttggatgatg gaagttagat cagtgtaaga atccgttccc gtcaagatat
    15721 cagagatagc atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag
    15781 gctgaccata gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta
    15841 aatagccacg gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag
    15901 gagtgaaaag gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag
    15961 gatcactttc gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt
    16021 cgacttggtg ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc
    16081 aacctacttt ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc
    16141 aacgttctaa aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca
    16201 attcttgaga ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata
    16261 taaaatatga ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg
    16321 agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtt
    16381 aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac
    16441 gcagttgcgc gtggcttggc aagtaagaaa acaacaacag ttggtgtaat catccctgat
    16501 gtcagcaata tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac
    16561 aaatacaata ttatcttagc aaattcagat ggcaacgacc aaaaagaagt caatgtattg
    16621 aataacttac tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac
    16681 gaaatccgtg gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat
    16741 ccagatgaac aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa agatgcagta
    16801 aacaaattgg caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat
    16861 cccatcaatg gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta
    16921 ccatatagcg aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta
    16981 gtcgatcgga tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc
    17041 atcggtattt tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc
    17101 atcacaagta acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca
    17161 caaccattat acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa
    17221 gaagaagtcg aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca
    17281 aaataataaa aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc tgcgttacaa
    17341 ggagcaattt caaaaaacag catctcatgt ttgatgaata ttgtgactcg tgattcggag
    17401 ttggcaggag aacaccgttt atttgggaaa aggggttatg acaaaatttt gttataatcc
    17461 tttttttgtg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg tatataaaac
    17521 aatccgtgta tagatgctgc attttgttca ccaccatcaa aaaaataagg gactgtgaca
    17581 atactattgt cacaatccct tattactgtg tattctatta aatcaatcat ctttctcatc
    17641 tgattcttta gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt
    17701 atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg gtccattttt
    17761 cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat tgacagtaac
    17821 ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat cagatgataa
    17881 ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt gattgatcag
    17941 attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag tattgtcatc
    18001 gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc atgaatctcg
    18061 gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat ttccgtttaa
    18121 gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac tacgcatcat
    18181 gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc tttcgtgttt
    18241 ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt cttcatagac
    18301 gccgccgttt gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga catctactgt
    18361 acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca tctttgccaa
    18421 gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt gatataagtg
    18481 ataagccggg atattgttgg accattctag tgactcacgg acggtttgga aggtgttcga
    18541 tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt gccaagttgt
    18601 tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg caggtccata
    18661 aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg catggttatt
    18721 ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag ttctgttatc
    18781 catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca tgtatccata
    18841 attttcaacg ccattttgca atgcagcata gacatcttta tcgattgtcg aatggattgt
    18901 atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac tgaccgatga
    18961 atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc ttgttgcttc
    19021 attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt gttgtggtag
    19081 aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg taatttggcc
    19141 ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag aaagatcttc
    19201 ttttagcgcg cctgtgttcg tgtatggact atacgtgatc gggctttggg gtaatccagc
    19261 gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga ttccttttgc
    19321 agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg gtgagacatt
    19381 caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg cagaaagaat
    19441 gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct gtttgactaa
    19501 ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag atactaatgc
    19561 tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat cctctgtgct
    19621 gataattgca gttttcaata aaggtgaaat cttatcagat gaaatagtcg tgcgcatcag
    19681 atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg acgtttctgt
    19741 aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag
    19801 ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca aaagaagaac
    19861 agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa caaaccattt
    19921 ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa gggtcacctc
    19981 aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac agacaaggct
    20041 gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga tctttattat
    20101 aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga acagggcaaa
    20161 gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt gttagtaaca
    20221 attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc atgagcggaa
    20281 agaaaatgct gttaggagca gcagcagaag cagccagtta tggatcttcg acttttatga
    20341 tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg aatattgatg
    20401 ccggaaaagc gtacatggca gaaaacggtc taacaaatat cgtcgttcat gccccttata
    20461 tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc caatttctga
    20521 gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt cacccaggtg
    20581 ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc ttgaatgaag
    20641 ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt aaaggtacgg
    20701 aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa ttaaatgaaa
    20761 aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat attaaagaag
    20821 acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg cttaaagtac
    20881 tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac gccaatattg
    20941 gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca atgtttgaca
    21001 atatttcgaa aatcttggaa actccctatg tcggcaaaga taaaaagaaa gcttacgcac
    21061 catatggaaa agaaatcgcc atgttcaaag cacaaatatt cgatccagaa gtgtttcctg
    21121 agttattagc agaagaagtg atactctaag attttctatc tgacagcagc aaaaaaggac
    21181 caggcacatt gccttgtcct tttttgttta agaaacaaag gagtactttt tgcagtcttt
    21241 ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacaaggta tactgttaga
    21301 aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa
    21361 gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata catactttat
    21421 tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta aagcaatcta
    21481 tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg ctcaaaatat
    21541 tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag gaatctccta
    21601 cagcttattt ttagtacttg cacataaact gcatctttct tatgctgttt tgttcggcat
    21661 gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgatcg tgaagaacaa
    21721 gtatcttctt ttaagtatct atttttattt tttgttttct ccgattagtt ttacacatga
    21781 atattccact cgaacatatc gtaatgccct cgtgattccg gctgttgaga tcattatcgc
    21841 ttgtttacta gctatttatt accgcaaata cggttctagc agaaaattgc tgccatggtt
    21901 tttagggctt tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt ctatctggct
    21961 tcttcctttt actgtagtag ctttgggtat cagtttcgtc cagatcttgc tagggcaaga
    22021 cttttctttc aaagaagtaa agaagttaaa aaaacgactc tttcttctct cgaaacggga
    22081 atggctgaaa ctttcattgt ttttacttcc tatagttggc ttttccatcg tctatcagtg
    22141 gatcgaatat aaaaatgaga caacatacgg tattgcaacc atcaatgacc gttccagtgg
    22201 agaatttgga cgtttgatga aacaactgat ccgaatagat gatgaaacga acttgaataa
    22261 agaaaacagt gagatatggg tctcacatgc tgcactaaat aaagcattag ccgcatctcc
    22321 tacattccat actgtttcta cccgtattga cgaattatat acgactcacg cgtggactca
    22381 aggtggaaaa gtaaaagaat tacccggaga tatcatcttt tgggcattgc gagatgtggt
    22441 aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt
    22501 caaccaagaa ttagaagaag cctatcacaa gggaaccatc aaaaagaaaa aagagttcta
    22561 tctgatggga tcaggagatg gaaagacgat gagtgatctg ccaatcgtat tcgatttcct
    22621 tcaatcagga tacgcttatg catggcgata taaagatttc catcaaggag gaaattatag
    22681 tttcggaacg caagaacaag tcaaacaggc tcaagaatta ttaaaccttc cgctaatgga
    22741 gaattggatc gatagtaatg aaccgagaac acctaatttc caattaacaa aatcagcaaa
    22801 agtcgcgaat gtcatcataa aaatttacca agttctggct attcccgtgc tgattgcttc
    22861 aatagcaggg atattactta tgatactagg aaccgtattt gcaaaagctg atcgtgccta
    22921 ttatagttcc tctttgattg tggtcaccgg attaatactg acacaattgg tttttctttt
    22981 cggtgtctcg tggttttgtt cttatgcgcc agaaaaaaag gattattttt taagtgtcta
    23041 cactggcgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc tagttttcag
    23101 taaattaaat ctgaaaaagg ctttatcttc taaataaaaa gacccttggt agctagattt
    23161 ttaacctaac tatcaagggt ctttgtgtgg aaaaatcagt ttctattcga tttcttctac
    23221 cgtttcctgc aaacgctgtt catatttttt gtttccttta tataaatcac caatcgtcca
    23281 tgccaacagg agaaggacaa gaacaatgat tacataagcg atgtgatcag ccaatgctag
    23341 ttgagcagaa gtggcttgtt caccaaagaa actttcgatc tgtcctggta atcctcgtaa
    23401 gttcaagtaa gttagactaa tcacagagaa ccagccaaga agttttacga tgaatgaatt
    23461 tttaaatcgg ctacccatct ccgcttggct gttggtcatc atcagtaaag ggatcatcga
    23521 aaacggcaac gcaaaagcca agaacacctg ggattcattc atcaagttat tcaaagcggt
    23581 atgttcttcg atcgttcctt ttgtacttgt aaacaaaaca caaatcaata caggaatcac
    23641 agaaagcaat cgtgtgacca gtcttcttag ccaaatcggg atacgcatat ggatgaagcc
    23701 ttccatgatg acttgaccgg tcaacgtacc agtaatagta gagttctggc cagaagcaag
    23761 aagtgctaca gcaaataaag tggataagat accagatttt gctacagcaa tcaagatacc
    23821 attgctcata gctgatggat cagataaggc ttcatacaga ccaaaaaagg aaggatcttg
    23881 gacagaaccg gttttgaaga cagcgacacc catgatcaat agcagtgcat tgactagaaa
    23941 agccatcgac aattggatat tagagtccca agtagaaaaa cgtacagctt gtgctacatc
    24001 ttcttcgtct tggtgatcaa tcttccgagt ttgagaaact gctgaatgaa gatataagtt
    24061 atgcggcatg actgttgcac caataatccc taaagctcca gtcagcggag tttcgccgcc
    24121 gatgaccggt gagtctgaga atgtatgact gttagggata aagccagcaa ataaactgtg
    24181 ccaatctggg ttagacaagc caacttgata gacaaaaacg aataaaatga cgaaaattaa
    24241 gcagacaacc agtgcttcga tcttacgaaa cccgactttt gtcagtagta agagaacgaa
    24301 aacatctaat acagtaataa agacggcaat caccaatgga atatcaaaaa gtaagtagag
    24361 ggcgattgct gcaccaatga cttctgcgat gtctgttgcc ataatggcta attcagtcaa
    24421 gatccataaa atgacaccca atgtcttact tgtgcgtgct cggattgctt gggccaaatc
    24481 catttgggtc acgattccta attttgctgc catatactgt aaaagcatag caatcagact
    24541 agacatcaaa ataactgaca tcaataaata ttggaaattt tgtccaccag tgatcgaagt
    24601 ggaccagtta cctggatcca tataaccaac tgctactaat gcacctggtc cagagtaagc
    24661 aagaagtgtt ttaaaaaaac ttttcccctt tggtacatca actgttccat ttacttcagc
    24721 taaagataaa ccatttgtat gttcaataag tcgatgctta tgaaattgtt tgttggattg
    24781 attattcaag aaaaaaccac ctttcctatt tatctacgat tacattctac acctcaaaaa
    24841 gaaaaaatta aagaaaaata ttcggtatgc ctaatttatt ttcgtggcgc gtcttgttag
    24901 atgacgaaag aaattttgtt ctcctctaaa aaaaccacta ttttttgcat gaaactaaag
    24961 aatacgcctt tcttttttca ataaacttta gtaaaataag tcattgagag caattcggaa
    25021 atagaaagga gagatttata tgttatcaac aggtattgtc gtacttattg ctattattgc
    25081 tttgctaatc ggcgcagtag gtggcttttt cctagcgcgt aaatatatga aagactattt
    25141 agaaaaaaat cctcccgtta atgaggacat gttgcgatca atgatgatgt caatgggaca
    25201 aaaaccatct gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa
    25261 ataggttctg acccaacgac tttttacggg ctgatttttt cagtccgttt tttattttgt
    25321 aatttgttag actattcaga ttattcagca aaaagacaag cacagacaat cctaggctct
    25381 tgataaagac aaaggaggag taaaatgtcg atttttagaa agttaggctg gttttttcgt
    25441 caggaaaaga aacattattt aattggggtt ttctcgctat tcgcagtggc cttggtacag
    25501 ttagttcctc caaaagtgat tgggatcatc attgatgaga ttgccgataa aaatttatca
    25561 gtaaagatca ttttcgggtg gatcttccta ttgattatcg ctgctttctt acaatatctt
    25621 tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcggttgga gaaagaactt
    25681 cggcagcagt tattcaaaca ttttacaaaa atggacaatc tcttttacca aaaatatcgg
    25741 actggggatt tgatggctca tgcaacgaat gatttgaatg cgatccagaa tgtagcagga
    25801 gctggtatct tgacctttgc agattcagtg attacgggcg gaacaacgat cattgtgatg
    25861 attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc tttgctggcc
    25921 gtcacttcac gagtgctggg gtcgaaactc catgatgctt ttcgtgattc gcaagaagct
    25981 ttttctagta tcaatgataa gacacaagaa agtatcactg ggatcaaagt cattaaaaca
    26041 tttggacaag aaaaagaaga tctagcggat tttgaagaaa agattgaaga tgcaattatt
    26101 aaaaacaaac gagtcaattt tctagactcc ttatttgatc cattcattac cttgattatc
    26161 ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga aggcacgatc
    26221 tcgatcgggc agatggtctc atttattagt tatatcggta tgcttgtttg gccgatgttt
    26281 gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga tcgcgtcaat
    26341 gaacttttgc atgaaaaaac gcatatcgtg gaacaactgg gtgcagtgca gaccccggta
    26401 aaaggcgagc tgaatgtagc tatttcctct tttaattatc cgaaagaaga atcaccagca
    26461 ttagaacaaa tcaagtttgc aatccaagaa ggagaaacat taggtattgt tgggaaaact
    26521 ggtgcgggaa aaacaacgat catcaaatta ctgatgcgtg aatacgatga ttatcaaggg
    26581 aaaatccaat ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt attaggatca
    26641 atcggctatg tgcctcagga tcacttcttg ttttcgatga ccattcgaga taatatccgt
    26701 ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt agcatttatc
    26761 aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga acgtggagtc
    26821 tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat caaaaatcct
    26881 gaattattaa tattagatga tgcactttct gcagtagatg ccaaaacaga agaagctatc
    26941 ttgcataatt taaaagaaat aagaaaagag aaaacaacta tcattgcagc tcaccgttta
    27001 agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat cattgaacga
    27061 ggaacccatc atgagctgct tgcactaggc ggctggtatg aacaaatgtg ggagaaacag
    27121 caattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat caatcggaat
    27181 ggacaaaatc gattccactt aaagaacaga aaaagatcat caaacgactg atgaagttcg
    27241 ccaaaccttt tagaacaacg ttcatcgttg ctatcttatt tgcgtttgct ctgtctgtga
    27301 tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg gctaaacagt
    27361 cggctacgac acaagttatt ttgttttttg ctggtatgta cttatttgga gtcctagtaa
    27421 aaagtatcat ttggtttttc caatggtatt tgtattccat ggcttcattg aagacctatc
    27481 agtatattag ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt tattttgatc
    27541 agacaccggc aggatcaacc gtttctcgag tgacgaatga cacagaaacg ctatttgaat
    27601 tttggtacgt ctttttgatg gtattgactg ggatctttgc agtcgtttct tcattcattg
    27661 ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgttcttg ccgattttag
    27721 gaattgtcat ctggtattac cagaaattta gttctcgtat ttaccgaaat atgcgggaaa
    27781 aattaagtca gctgaataca aaattgaatg aatcgatttc cggcatgcgt attatccagc
    27841 agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaat gagtatttag
    27901 acacgcgcta tgcgatgatc aaaacgaatt cgattctgtt aagtccgatc atcaattttc
    27961 tatatgcatt ggcgattgcg ttggcgttga ctttatttgg gatcgatgcg ttgcattctc
    28021 ccgtagaagt tggactgatc tatgcattta cgacttatgt ccagggattt tttaatccaa
    28081 tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct ggtagtcgta
    28141 tcttgaagat tatggatacg gaagaatatg caccacaaca aaaccctgga gcagatcaaa
    28201 tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat ggagagcaca
    28261 atgtattaaa caatatctct tttgttgcta atccgggaga aaccgttgca ttagtgggac
    28321 acaccggaag tgggaaaagc tcgatcatca atgtgttgat gcggttttac gaattttatg
    28381 aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa gagttacgta
    28441 agaaaatggg gctggtcttg caagatgctt tcttgtttta tggggatatc gcaggaaata
    28501 ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct gagtttgttc
    28561 aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg atcgaacatg
    28621 gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca atcgtgactg
    28681 atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa acagaaggat
    28741 tgatccaaga aggattggcg aaaatgcgac aaggacggac aacgattgcg attgcccatc
    28801 ggctgtcaac gattcgagat gcagaattga ttttagtttt agacaaaggt catatagtag
    28861 aacgcgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat atgtacaaac
    28921 tgcaaagtag tggtatataa aagatgttgt gacagtagca ttttgaccta agcactagag
    28981 tgaaaataaa acatagcttc ccatattttt catctgtgag agttagtgtc gtagttacca
    29041 gatttaaaaa agacgacgtc agctagtcag aagtgcctaa tagcccctaa gaaagcgttg
    29101 acaaaatcgt ttgctttcga taatataaag aaaacgatac aacttggata gtgattgcct
    29161 gatgcaagct aaacctgatg agtctcccat ggtttcatgg acggcttttt gggtttttct
    29221 ttttgttttg gatacagccc tatatatcat ggaaaggagc aaaacatgct gattgagaaa
    29281 gtactgaata acaacgtcgt aataacaagg gatgaaagag atcgggaaat gattgtgatg
    29341 ggaaaaggat tggcattcaa aaagaaggtg ggtgacccga ttcttgactc atgcatcgac
    29401 aaaaaatttc agttgacaaa tgacggactt tcacaacagt tccaagaatt ggcacaagcg
    29461 attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctctg
    29521 caagtccatt taaatgattc tgtgtatatt tcattgacgg atcacttgta tacagctatc
    29581 gaacgtcaaa aaatggggat ctttgtgcca aatgtattgc tgtatgatat caaacaactg
    29641 tttcctaaag agtatgccat tgggaaaaaa acagtgaaga aaatctcgga taaataccac
    29701 atcaagttat cagaagacga agccggattt atcgcattgc atttagtcaa tgcacaaggc
    29761 attcaaagcc agcaagacaa tacctacctg tttaccacaa caatacagga tattttaaat
    29821 atcgtccgct attactataa gattacattt gatgaagatt cagtccactt ttttcgattc
    29881 gtcacacacc tgcgtttttt tctgacacgt gtcctagatg gagcgaaatc agaagatgga
    29941 gaagtttctg aggaattgtt ccaagttatt caaaaaaaat ataaggatac gtttggttgc
    30001 gttgagaaaa tagcagactt tttaaccggg gctttcgact accagatgtc cacggatgaa
    30061 aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaata
    30121 aaaaaggagg acaaattaac aagaacgtat gaaataaggg tagtgacatt cagttggcta
    30181 aaccttccac taattaattg gagggaatag aaatgggaaa ataccaggaa ttagcagaaa
    30241 acatcgtaaa gaatatcgga ggaaaagaaa atatccgtag cttgacacac tgtgtgacgc
    30301 gacttcgttt caaactaaag gaggaaaata aagcaaagga tgatgttttg aaaaagatgg
    30361 aagggatagt caccatcatg aaaagcggcg ggcaatatca agttgtaatc gggaatcatg
    30421 tgccagatgt ctatgcagaa gtactgacag tagccgggat ttccgatgaa gcagaagaag
    30481 aggtttcttc aagtagtaat ctgttcaatc gattaatcga catattaagt ggctgtttcc
    30541 agcctttttt aggtgctttg gctgcagcgg gaatggtcaa aggattaaat gctttgttct
    30601 tgtttctcaa attttatact gaaggatcgg gtacgtacat catgctgaat gggatagggg
    30661 atgcaatttt ttatttcatg ccagtgatct tagggtacac agcagcgaaa aaatttcatc
    30721 tgaatccgat ggtggggctc gtgattggtg cagcactttg ttatccgact gtacaaggag
    30781 cagcactaca aagtggtttc cctgatggca gtcctgcgcc ttatagcttg ctgggtcttc
    30841 cggcttatga aacatttttg ggtatcccat gggtgggcag cagttatact agtagcgtcg
    30901 tacctgttat cttcattatc gcatttgccg gacaagtcca aaaaattgcc aagaaatgga
    30961 ttcctgcagt cgtccagacc tttttggttt ctttttctgt gttattaatc gcacttcctg
    31021 ctggcttttt gattatcggt ccgattgtca gtatactgac agatctatta agcacgggat
    31081 tccaagcatt gatggacttt tctccagctg tgtatggtgc agttctaggg tttctttggc
    31141 aagtactggt gattttcgga cttcattgga gtattatccc tcttgcgatt atgcagctag
    31201 cgcaaaatgg gtggagtcag atgttagtag gcgtttatgg cgcgagtttt gcacagacag
    31261 cagtggtatt ggcgatgtat ttcaagttca aggatcaaaa aatgaaggcg ctttgcatgc
    31321 cagctgtgat ttcaggaatc ttcggcgtaa cagaaccggc tatttatgga atcaccttac
    31381 cgaagaagaa gccatttatt tactcgatga ttggtggtgc tgtttcagga atctatttga
    31441 tggcatctgg aacaatcagc tacataatgg gcggattagg gatttttggg gtggtaaact
    31501 acatcacgga aacagacgcc agtggtatgt ttcaagcgtt tatcggcatt gctattgctt
    31561 cggtaatcgg gttttcgctg acttttttct tttggaagga tgaagcaggt gaaacagatg
    31621 ctatacagaa aaaatcaggt aaagaaattg taaaaacacc gatacaagga caaatcgctc
    31681 cactaaatgc agcgaaggat gcagcttttg cacaaggaac actaggtcga ggaatcttaa
    31741 tctaccctga aaaaggagaa gtacgtgccc cttttgacgg aacagttatg accctgttcc
    31801 cgacgaaaca tgcaatcgga atggtttctg aaacagggct ggaattgctg attcatgtcg
    31861 gattggatac tgtgcagttg gaagggaaat attttgaaag cttagtgcag caaggagaaa
    31921 gagtgagaca aggcgatctg ttgctacgct tcgacaaaaa gaaaatcgaa aaggaaggat
    31981 acagtttaga aacacctgtg atcgtcacca attacacgga ttatttggat attttggaaa
    32041 accaagggga gacggtcggg ccagacgatg cacttatcac cgcactgacc tgaacggtta
    32101 ggtgaagaat ggactaggct taactattct ttttattcca cttctttatt ttcaaaattg
    32161 aaaggctctt tcatgatggc tgagctgata gggcgtttat cgaatttttg accgacagaa
    32221 attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct
    32281 agaagataac aaattgatgt attgatcgta aaattaccaa tttttgaata cagtcgctcg
    32341 cgtgtcgtaa gtcgcaaagc ggctgtactc attgcagata gcgttgaatc accgatactg
    32401 gtcaaagcta aaattggaac atcattttgt ttcaatattt ctgcggtacg aaggatcaca
    32461 ttgttttcac ccgtataaga aatcagaatc ccgcacatat cgctcgtaca attgtacgct
    32521 tcatagaatg attcaccaaa agtggtgaca attgtgacat ccattttgat gcgcttcatt
    32581 ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaagat cacgattttt
    32641 tttgcctgga gcagtaaata tttagctttt tgcagttggt catgatgaat gagagaaagt
    32701 gtatcatgaa tcgtcatctg ttctaaagaa gccattttat ttgcgattgt cataataccg
    32761 tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct
    32821 gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct
    32881 acacggatga gtgtagacgg gtggacataa catcgctcag ctatcgcttg gatggtcatt
    32941 tcgccaagtg ctggagaatc gcttaagaga aactggatca cttcattttc tgaaggagaa
    33001 aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttattttttt
    33061 acgtaaatta taactttttt gaaaatgttt tgtaaacg
//
LOCUS       DAXWLH010000009        32937 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXWLH010000009 DAXWLH010000000
VERSION     DAXWLH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.009"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(275..613)
                     /locus_tag="ACGE36_000307"
     CDS             complement(275..613)
                     /locus_tag="ACGE36_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFF8382736.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(748..1149)
                     /locus_tag="ACGE36_000308"
     CDS             complement(748..1149)
                     /locus_tag="ACGE36_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382737.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            1267..1881
                     /locus_tag="ACGE36_000309"
     CDS             1267..1881
                     /locus_tag="ACGE36_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8382738.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            complement(1972..4641)
                     /locus_tag="ACGE36_000310"
     CDS             complement(1972..4641)
                     /locus_tag="ACGE36_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFF8382739.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(4879..5067)
                     /gene="rpmB"
                     /locus_tag="ACGE36_000311"
     CDS             complement(4879..5067)
                     /gene="rpmB"
                     /locus_tag="ACGE36_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFF8382740.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            5337..5699
                     /locus_tag="ACGE36_000312"
     CDS             5337..5699
                     /locus_tag="ACGE36_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8382741.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5751..7433
                     /locus_tag="ACGE36_000313"
     CDS             5751..7433
                     /locus_tag="ACGE36_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFF8382742.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            7550..9586
                     /gene="recG"
                     /locus_tag="ACGE36_000314"
     CDS             7550..9586
                     /gene="recG"
                     /locus_tag="ACGE36_000314"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFF8382743.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALTGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            9635..10636
                     /gene="plsX"
                     /locus_tag="ACGE36_000315"
     CDS             9635..10636
                     /gene="plsX"
                     /locus_tag="ACGE36_000315"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFF8382744.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10758..11000
                     /gene="acpP"
                     /locus_tag="ACGE36_000316"
     CDS             10758..11000
                     /gene="acpP"
                     /locus_tag="ACGE36_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8382745.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            11268..12272
                     /locus_tag="ACGE36_000317"
     CDS             11268..12272
                     /locus_tag="ACGE36_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382746.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            12273..13217
                     /locus_tag="ACGE36_000318"
     CDS             12273..13217
                     /locus_tag="ACGE36_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382747.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            13217..14179
                     /gene="opp4B"
                     /locus_tag="ACGE36_000319"
     CDS             13217..14179
                     /gene="opp4B"
                     /locus_tag="ACGE36_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8382748.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            14206..15120
                     /locus_tag="ACGE36_000320"
     CDS             14206..15120
                     /locus_tag="ACGE36_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8382749.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            15146..16927
                     /locus_tag="ACGE36_000321"
     CDS             15146..16927
                     /locus_tag="ACGE36_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8382750.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            17275..17961
                     /gene="rnc"
                     /locus_tag="ACGE36_000322"
     CDS             17275..17961
                     /gene="rnc"
                     /locus_tag="ACGE36_000322"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFF8382751.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17981..21562
                     /gene="smc"
                     /locus_tag="ACGE36_000323"
     CDS             17981..21562
                     /gene="smc"
                     /locus_tag="ACGE36_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFF8382752.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            21571..22380
                     /locus_tag="ACGE36_000324"
     CDS             21571..22380
                     /locus_tag="ACGE36_000324"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8382753.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            22392..23390
                     /gene="ftsY"
                     /locus_tag="ACGE36_000325"
     CDS             22392..23390
                     /gene="ftsY"
                     /locus_tag="ACGE36_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFF8382754.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            23577..25844
                     /gene="gshAB"
                     /locus_tag="ACGE36_000326"
     CDS             23577..25844
                     /gene="gshAB"
                     /locus_tag="ACGE36_000326"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFF8382755.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWKEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25932..26384)
                     /locus_tag="ACGE36_000327"
     CDS             complement(25932..26384)
                     /locus_tag="ACGE36_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFF8382756.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            26534..27484
                     /locus_tag="ACGE36_000328"
     CDS             26534..27484
                     /locus_tag="ACGE36_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8382757.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            27477..28436
                     /locus_tag="ACGE36_000329"
     CDS             27477..28436
                     /locus_tag="ACGE36_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8382758.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            28433..29188
                     /locus_tag="ACGE36_000330"
     CDS             28433..29188
                     /locus_tag="ACGE36_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382759.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            29211..30164
                     /locus_tag="ACGE36_000331"
     CDS             29211..30164
                     /locus_tag="ACGE36_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8382760.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30808..31104)
                     /locus_tag="ACGE36_000332"
     CDS             complement(30808..31104)
                     /locus_tag="ACGE36_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFF8382761.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            31460..31816
                     /locus_tag="ACGE36_000333"
     CDS             31460..31816
                     /locus_tag="ACGE36_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8382762.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31826..32725
                     /locus_tag="ACGE36_000334"
     CDS             31826..32725
                     /locus_tag="ACGE36_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8382763.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        10897 a         5846 c         6916 g         9278 t
ORIGIN      
        1 aaaacattta tattttgccc tacttatttt tttaattaaa ttcattgatg attcatattt
       61 agaaaattct atgaaggcca aatgagtatc ctgtcacttt tttagataaa tagcaaaaat
      121 tttcagcgtt aaatgacaaa taattagaaa tatttgtgcc atttaaatat aaaaatgaca
      181 ccaagtatat catgaacgca caaaaagacc aacagggtat accctgttgg tctaaataaa
      241 tgtcaaattc cgttctcaac tgtgcatatt cattctatgt tccatgtacc ccattatcct
      301 cctgaatact cattccaatg ttagttaatt tttctaattc ttctacgctt agataagcgc
      361 catattttac aatttcctca tcaatgacaa taatcggtaa acttttcaaa tcttttgtct
      421 gcaacaagtt aagaatttta gggttgctaa taaaaggcaa gatagagtca cttaaaaaat
      481 ataaatttgt ttctatctgt ttattcttaa tccgattata aaactctttt agcatcgtat
      541 cctcatgtaa tgattgaaaa acgaagagtc cttcatcatc acttctattt aagacatagt
      601 aagaaattat cattttccta ttgtcaatcc tcccgcagtg caagagactg accctacacc
      661 aaatgtatta taaacttgca tagctaattt tacactgtaa tattgtcttt tcccagtaaa
      721 tgtaaaatac attcctccac cataacctta agaattagct agatttactg tttgagtata
      781 tgattttgca ctacctaaac tcaagcgtgt agaaatacga tacatcgccg aattgggact
      841 tgttataata tattctgtcc gcccctgcgt attagtaatt tttttagtag atgcggttaa
      901 ccgcaataca tttgcagaaa aacgtgtttt cggatcttga ggctctatct tttgagtgta
      961 aaaagtatct ttatcactaa atcctttgga tttgaagtaa ctttgttctt ctggtgttaa
     1021 ttgactaaaa gtttttgcca tttcacttgt ttgttcaacc atcgtgctag ctttaactat
     1081 atttccagaa gtcccaatac caataaagcc aaataacaga ataaaaagtg cgctaaataa
     1141 ttttttcata gtaatcactt cctaattcat taaattttta ggaaaacgct ttctaaatct
     1201 cgattataca tctcctttta aaaatgtaca ccgttatttt ttgaatattc agactttttc
     1261 ttgttcttga taaatgcaac agatgaaata ttaagtgtaa ataaagtttt ttatactgta
     1321 ccagctgaaa agatgtctcg tgtcagttcg gatattaaca caataactag tagaataggt
     1381 ctgactaaag caatgcatat ggtactaaaa tacaatcaag aagatcctcc tgtgcctaaa
     1441 acctatgaag caattgactt aattattgtc gatgaaattg atcgtttaaa gcttcagcat
     1501 ttagaacagc taagagatat ttacgataga aatgatctgg caatggttct tatcggtatg
     1561 cctggtattg aaaaaagatt gtctcgttat ccgcagcttt actctcgcat aggatttgca
     1621 catgaatttg ataaccttag taaggacgaa acacatcata ttttggaata caaatgggca
     1681 gatttaggaa ttgatatcaa gctagaagat ttttctgatt atgaggctat cacaactatc
     1741 attaagatca caaaaggaaa ttttaggctt attcatcgtt tatttgcgca gattgatcgg
     1801 attttaagaa tcaatcaaat ggataagatc actgtagaag ttgtcgaagc agcaagagat
     1861 agtttagtca taggtatata aaaaaccacc aaaaatcagg tggtttttgt catttaaaga
     1921 ttgaaatttt tgccatttaa atagaagagt gacacagatt ccatcgtttc tttattttct
     1981 tcgtcttctt ctgtgtgtat ttcgcggttt ttcttctatt gctggatcaa tcgtgccata
     2041 aaaggggcga ttgcggtcaa ggtaccagac ataaagaata aacaatgcaa tacataggac
     2101 aaaaaggatt gcacctatcg taaatccttc aggcaagtaa ctgaaagtga cagtatgttt
     2161 acctgctgga acttttatca tcaagaatgc gtctttgaaa ttgctgacct tgactggttt
     2221 cccatcaatt ttagctttcc atcctttgtc ataaggaata gttgtcacca acatggaatc
     2281 ttcagcggta tttacagtcc cagaagcttt tctgccagtg gcttccattt cgacaccttt
     2341 ttctttgatc tgattcattg cggcttgata ggctacaaca tctagaccaa cgactttagg
     2401 ctccatgaaa cttacttctt tcgttccata aaaacttgca gtaaatgtca ctgttgtctc
     2461 ttgaggataa tagccaagat catagtattg accagaaatg ttgatttgcg tttttcgcga
     2521 tgtaccattg acagtgatcg tcgcactaga actttctaat tgtgcaaaat tggttgggaa
     2581 caaacttaag taagcttgtg tatttgcagg cacatggact tcccaagtga gctcttttgg
     2641 ctggttatct gctgtttctg agtaagtgac gccagtcgtt gtctttgtga tcgtcgtatt
     2701 caactgattc ttcaaagtag gtgtatagaa tgtaaaatac tgttcattta aaccagacaa
     2761 tgtattgatc aagtttgtct gactggtcaa attatctgtc actggctgct caacatcatt
     2821 aacggacaac ggtgcagtaa atgctagtgg caatgcattg acattctgat acagtgtata
     2881 gtcgccactt tgaccagctt ggcggaagcc atatttactg aacgtgcttt cgtttttaga
     2941 aatgttatat ttgattcctg taaacccatc cattagcaaa gtattattgt tataacggat
     3001 attcaaattt gtccctcttg aacggaaacc taatttatcc aggtaagagg aagaattacg
     3061 atttcttata gaagaaaaca aactgatacc actgtaacca tagttgatcg aatcgtttgc
     3121 agatactgga tccagatttt ccaagcggta aaacgagtcg gaatcttttt ttgtttcatt
     3181 tacaagagct ttcaagtcag gataaggatc agtatataaa ctgcgggaag cataattcca
     3241 atcatcaaga atgccatgga tcatactgtt cgtattgacc agtgcttcag cagacatgat
     3301 caataataac aatatactca gatgattgaa tgaaagtttt ttatgctgat aaaatcccac
     3361 agcaaaagca taaatcccta agaaaaccac tgtcaataca aaggaagtca tcgtcacata
     3421 ggtgtagctt ccttcaggtt tgatgcccca tgccaccgca aagagagcag ctacgaccaa
     3481 aatgattcct aacaacagcc cacgatcgtc ttgttctatc ttttcccatc cttttcctgc
     3541 aagcaggagc actaagaaag agaacagata ggcataacgg aataaaaaca tattcggtgc
     3601 atgcatacca tgccagaaca aattcaatgg aacaagatag aaacttgcga taagtatggc
     3661 gaaaagactg ccgtacaaaa atttattttt caacggtact ttccgactgg caaaataaaa
     3721 cagacagagg actaatggta gcagacctgc gtaaacgaac ggaatcgatc cgtattttgt
     3781 cgtgtcataa acaccaatca tatttttcat gattagatcc agataagaag tcgcttctgt
     3841 tttcaacgta gtgatctgac tcagtgcctc gccatttgac cgaagatcca gcactgctgg
     3901 caatacaatg atcattgaag cgccgcctgc cagtaaagaa gtgataccat aaggaacaat
     3961 cgttttttta tattctgacc agtttgtcaa tagacgggcg ataaaatata agaaagaaaa
     4021 tacgccaatc ataaaaccca tataaaaact ggataaaaac aagagtaaat aactgataaa
     4081 taaaagtttc ggccgtttaa gatccataac acgatgaatc cccaaaatca ctaatggaag
     4141 ataaacaaat gcatccagcc acatgatgat ctccgaatgt gcagtgataa atgagatcaa
     4201 tgcgtaactg acgctaagtg caacatgccc ccagcgagga atcttgaacg tacgtgcaag
     4261 aaaccaaaaa gccaatccgg cacagccgac cttaagcaaa gtcagaacat acagtgcatc
     4321 tggcatcatc tggtttggaa aaaagaacac cagtggtgtg aagatcccgc ctagatagta
     4381 agaaattaaa gataaataat tcaatcctag agaagcattc catgtataaa aaatactttg
     4441 ctttccaaga agaacatttc ggaagctggc atgaaaatta gaaaactgtg aaaaggcatc
     4501 acttgccaaa atacttctgc tgcttccagg atagatcccg atacttagat aaacaagtgc
     4561 taaaatcaaa aacggaatca ggaaactagc tgctgcgtag cgtccgtttt ctctaaagaa
     4621 tgactttaaa ttttttttca tatttatttt ccaccaaatt tctatgaagt tgtattcagt
     4681 ttatcatatc cttgtttttc tctgcttaaa aagagtctaa ttttcacaaa aaaataaaat
     4741 ttagaataaa taacttcata atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag
     4801 gaatcaaaag tcccaccctt aaaaattttt attaaataaa aaaccgttca ctatctgaac
     4861 ggtccaagta tcattcaatt aaacgcgttc aacttttcct gatttcaaag cacgagttga
     4921 cacccaaact tttttaggtt ttccgtcgac cataacgcga actttttgta agttaggttt
     4981 aacagtacgt tttgttgagt tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg
     5041 acctgtaaag taacaaactt ttgccatggt aatttcctcc tttgccttga tttggagcaa
     5101 tagaataatt cagctcatac cagactaaca tatcatatag aatggaaatt tgcaagtaca
     5161 ttttcataga tacaattgac aattgagaag aataattgta aattaaacaa ggttatacct
     5221 gaaattttgc aaggtttttg agtactttgg gtaactgcct tttatttcaa gttgcgctat
     5281 gataaaatat gatagaagaa aaatgggagt tttttacccg ttaaggaggc tcattcatgg
     5341 ctgtaaaaat tcaaacgtca gctggaacca ttgaaattac caatgatgta attgcaacag
     5401 ttgttggtgg cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa
     5461 ttaaagataa tattactgag attttgcgta aagaaaatta ctcaagagga gtagttgtcc
     5521 gccaagaaga taatggtatt gctgtagatg tttatacgat tatcagctat ggtacgaaaa
     5581 tctctgaagt ttcccgcaac gtccaagaaa aagtgaaata caatctagag gcattattag
     5641 gcgtaactgc caattcagtt aatgtctttg tacaaggtgt tcgtgtactg ccagactagg
     5701 caggagactg gctggtaact cagcagtcga tctgaaggag gattatctag gtgagtgtaa
     5761 cagaaataag tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag
     5821 taaatgcaga atacgtcaat tcattaaatg tattccctgt gccagatggc gatacgggaa
     5881 caaatatgaa tctatctatg actagtggag ctaaggcagt agtagattca acctcagaaa
     5941 aagtaggaga attagcagca atgctttcaa aaggattgct gatgggtgcg cgcggaaatt
     6001 ccggcgttat tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca gatgttgtct
     6061 cattaaatgc ttccgatctt gctgcagcct tcactcatgg agttgaaact gcgtataaag
     6121 cagtaatgaa accagtagaa ggaacgattt tgacagtagc tagagaagca gcgaaagctg
     6181 gagaaaagaa agcaaaaagt actgatgatg tcgttgaagt aatgacagct gttgtaaaag
     6241 gggcaaagcg tgctttagct aaaactccag acttgcttcc agtcctaaaa gaagtcggtg
     6301 tcgtagacag tggagggcaa ggactgctgt ttgtttatga gggcttttta agtgcattga
     6361 atggagaatt ccaagcggat gaaacatatg aaccttctcc agcagaaatg gatgatatgg
     6421 tcaatgcaga acatcatcgc agtgtccaag gtcaaatggc gacagaagat atccattttg
     6481 gttactgtac agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat agtacattcg
     6541 attacgaaga attccgtaac tatttagatg gtataggaga ttctttactt gtggtcaatg
     6601 atgacgagat catcaaagtc cacgtgcata cagaaaaccc aggagaagtc ttgaattatg
     6661 gacaaaaatt cggttcatta gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga
     6721 cgattttgga acacgatgaa gaagtgaacg agtttgcgaa aaaagaaaca ccacgtatcc
     6781 cgtatggtat cgtagcgatc gctgctggta aaggcgtcca agagctgttt gaaagtttag
     6841 gcgctaatta tgttatcagt ggtgggcaaa caatgaatcc aagtacggaa gacattgtta
     6901 aagcgattca agaagtaaac gcagataaag tgattgtctt gccaaacaat aagaacatct
     6961 tcatggcagc tgatcaagca gcagaagtag cagatatccc agtagcagtt gttccttcaa
     7021 gaacgatttc ccaaggattg acagctatgc tttcattcaa tgatcaagct tctttagaag
     7081 aaaacaaaga aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg
     7141 cgatcagaga tacctcaatt gacggtgtag agatcaaaga aggagacttt ttgggcatga
     7201 tcgatggaaa aatcctgatc tctaacccag atatcacagc aacagcgtta agtacactag
     7261 aacaaatgat caatgaggac acagagatcg taacgatctt ggcaggtgat gaaggaacag
     7321 ttgaacaagc aaatgaattt gctgagaaac taacagaaaa acatcctgat ttggaaatcg
     7381 agatccacca aggagatcag cctgtctatc cttatctatt ttctgctgaa taatttctgt
     7441 gtcagaatat ctttctaaca cagtattcat tcataaaagg ccttcggtta gtgatcaggt
     7501 atgatcgctg accaagtcct ttttttgtta agggagggag gaaaaattta tgcgttcttt
     7561 agatgatcca atcactatgc tggcaggagt cggagaaaaa agagcagcta gtcttgccag
     7621 cttgggcatc caaacgattg aagacttact gacttattat cctttccgct acgaagatat
     7681 tcaggaaaga aatctaaacg agatccaaga tcaagaaaag gttactttga aaggaatcgt
     7741 agtctcgcct cctgtgatga atcgatttgg ttataaaaaa agccgattgc aatttcgtat
     7801 gatgcaagaa catgatgtgt tcaacgtttc tttttttaat caaccttatc taaaagaaaa
     7861 agtcatttta tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc gtaaagcatt
     7921 gaccgggatg aagattttag gttctcaaac aatggatgac ttttcgccga tatatcacgt
     7981 caacaaatca attcgtcaaa cgacattagt tgatctaatc aaaaaagggt ttcaagaata
     8041 cggcacattg attgaagaaa atctgccaaa caaccttgtg gagaaatatc gactgcttga
     8101 ccgagcttct gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa accatcaagc
     8161 caaacgacgt gtcgtttttg aagagttctt tttatttcaa atgcgtcttc aaggattgaa
     8221 aaaagcagaa aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa
     8281 agaatttact caaaagctac cttttgaact gacaaatgca caaaaacgtg taacgaatga
     8341 aatctgtcgg gatcttcgca gtccgcagca tatgcagcga cttttgcagg gagatgtagg
     8401 tagcggaaaa acagtggttg ccgcgattgc gttatacgct accgttacag caggtttcca
     8461 aggtgcgttg atggtgccta cagaaatctt ggcacaacaa catatggaaa gcttgcaaca
     8521 gctgtttgat cccaatgaag taaccatcgc attgcttacg gggtcaacga aagcaaaaga
     8581 acgtcgtgag ctccttgaaa aactagagca aggggaaatt aacatcgtga tcggcacaca
     8641 tgcattgatc caagatggag tcgaatttca gaatttaggt ctagttatta cggatgagca
     8701 gcaccgtttt ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc gaccagatgt
     8761 cttatttatg acagcgactc cgattcctag aacattagct attacagcat atggtgagat
     8821 ggatgtatct atcattgatg aacttccagc tggacgtatc ccgatcgaaa cacgctgggt
     8881 aagaacacct caattggaca gtgtactgga ttggacgaaa aaagaactgg caagaggaca
     8941 tcaaatgtat gtcatctgtc cgctgattga agaatcagaa gcattagatg taaaaaatgc
     9001 agtggaaatc tatgaaaaac ttcgtgaact atttgcacca aaatatgaag ttggcttgtt
     9061 acatggtaaa atgaagaatc aggaaaaaga agtaatcatg gaagccttca aagacaacca
     9121 gatgcagata ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac
     9181 cgtcatgctg atcatggatg cggatcgatt tggattggca cagctgcatc aacttcgtgg
     9241 acgtgttgga cgcggaagtg atgcttccta ctgtatctta gttgctaatc ctaaaaatga
     9301 actaggtgtg gaacggatga aaatcatgac agagaccacc aatggttttg tactaagcga
     9361 aaaagatctg gaattacgtg gtcctggtga agtatttggc ttccgtcaat ctggtctgcc
     9421 tcaatttgcg attgctgact tggtgacgga tggaaatgtt ttggaggtag ccagagaaga
     9481 ggctgcagcc ctttggcaga taaaggattg gcagatgctt cctgagtatc agcctttagc
     9541 gcaaagcctc caaatagaaa atcaggataa tcgttatttc gattagcaga ctgtgctata
     9601 ctttatactg atgacggaaa aaggagtgga aacaatgaaa attgccgtag atgcaatggg
     9661 cggagatcac gcacctcaag caattgtaga aggcgtggct ttagcgcagc aagattttcc
     9721 agaagtagaa ttcttacttt acggaaaaga agcagaaatc aagaaatact taacaaatga
     9781 aaaaaacatc acgattatcc atacagacga aaaaatcaat agtgatgatg agccagtaaa
     9841 agcaattcga cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg
     9901 tgaagcagat gctgtatttt cagcagggaa tacaggcgcg ctactagcag cagggttgtt
     9961 catcgtagga cgtatcaaaa atatcgaacg ccctggcttg atgtctacct tgccagtagt
    10021 agggaaagaa ggtgccggtt tcgatatgct ggatcttggc gctaacgcag aaaacaaacc
    10081 agaacatttg ttgcaatacg gtattttagg ttctttttat gcacgtaaag tccgaggtat
    10141 cggacgtcct cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt
    10201 gacgaaaaaa gcctatgaac tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt
    10261 agaagctcgt gagctactca atggtgtggc tgatgtagtg gtcacagatg gatttacagg
    10321 caacgctgtt ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc tattaaaatc
    10381 tgctattcta aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa aagacggctt
    10441 acgttctttg aaagcagaaa tggattattc caaacatggc ggtgccgttt tgtttggctt
    10501 gaaagcacca gtcattaaaa ctcatggagc aacaggcccc gatgctgttc gctatacgat
    10561 ccgccaaatc cacacaatgc ttgagacaga tgtagttgga caattagtcg aacaatttga
    10621 aaaaagcgaa gaatagtatt tggatatctg aagcaatccg ttccattgaa aaaaaggtgg
    10681 acattcctga aaaatgccag taaaattagc catggtgtag gtaattgaca agtgtatgat
    10741 tcttggaggt gaaaaagttg actcgcgaag aagtatttaa caaagtagcg aaaattattt
    10801 caaatcattt tgagatagat accgataaag tgacggatga attgaacatc aaagatgatt
    10861 taaaagctga ttcaattagt attatggaat tcgttttaga gcttgaagat gaattcggta
    10921 cggaaatttc agatgaagac gctgaacaaa ttgaaacagt cggtggcgca gtagattata
    10981 tcagcagcca tcttaaataa tttcaaataa aagcaaagaa ggataaaaag ctgcatacag
    11041 ctttttatcc ttcttttttt aatctttttg acacgtaaaa gtcatttttc tgataattct
    11101 ggcgataaac agaaattatg ttgcaaagta aagcgatttt ctatataatt attaataatt
    11161 cattacacaa agataagaat aacctttgtt ataaaacgct ttattctgtg taatgtcctt
    11221 ttttattaag aaaaacttcc atttaggaag cagagagggg tattcgattg tccgataacc
    11281 aattattaga agttcagaac ctacatacag gattccgact caaagatgac tattacgatg
    11341 cagtagatga cgtttcattt actttagaca aaaacgagat tttagcaatc gttggggaat
    11401 ctgggtgcgg gaaaagtacc ttggctacta cgattatggg attacttgat cctaataaca
    11461 caaaaattac aggtgagatt ttatataacg atctgaattt aatcgacctg aacgaaacgc
    11521 tctataataa aatccgcgga aatgatatcg gaatgatttt ccaagatccg ttgtctgcat
    11581 taaatcctct catgagaatc gaagaccaga tcaaagaagg actttcctat cacacaaaga
    11641 tgaatgcaga acagcgtcaa gctcgtgcac tagaattgct agaacaagta gggattccta
    11701 atcctgcgag agttggacgc caatatcccc atgaactatc aggcggtatg agacaacgtg
    11761 tcatcatcgc aatcgcaatc gcctgcaaac ctccaatcgt cattgcagat gaaccgacga
    11821 cagccctaga tgtaaccatc caagcacaga ttcttgattt actgaaagac cttcaggaag
    11881 aaacaaaaac agggatcatc ttgatcactc atgacttagg agtcgtagca gaaatggctg
    11941 atcgagtggc agtgatgtat ggcggacagt tcgttgaagt agcaggtgta aaagaactgt
    12001 ttgatcatcc taagcatcct tatacacgct ctttactgaa ctctatacca caagaaggaa
    12061 atcatgatgc agaactccat gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc
    12121 gtaaaggttg tcggtttgca ccacgtattc catggatacc agcaagcgat catgaagaag
    12181 agcctatcct tcatgaagtc gcaccgaacc acttggtacg ttgctcctgc tataagcatt
    12241 tccactttag agacgaaaaa ggagaggtgt agatgacaga attaatcaca atcaaagacc
    12301 tgaaagttca ttaccctatt cgcagtggat tcttcaatcg agtaacggat catgtatatg
    12361 ctgtagatgg tgtggatttt attatcgaaa aaggaaagac atatggttta gtgggagaat
    12421 ctggctctgg aaaatctact acgggaaaag ccgttgtggg attagaaaag gtgacagccg
    12481 gacaaatcat gtatgaagga aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt
    12541 ataacaagga tgtgcagatg atcttccaag attcaatgtc cagtttgaat cctaaaaagc
    12601 gagtgttgga tatcattgct gaacccatcc gaaattttga acggttaagc gatcaagaag
    12661 aaaagaaaaa ggtaaaagga ctgctagata tcgttgggat gccggaagat gcgctatata
    12721 aatatcctca tgaattttca ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg
    12781 ctaccaatcc aaaactgatc gtagccgatg aacctgtatc tgctctggat ctatctgttc
    12841 aagcgcaagt attgaatttc atgaaacgaa tccagcaaga atacggcctc agctatttat
    12901 tcatttcaca tgatttaggt gtagtaaaac acatgtgcga caatatcgca attatgtaca
    12961 aaggtcgatt tgtagaaatc ggtacaagag aagacattta taatgacccg cgacatatct
    13021 atacgaaacg tctgctttca gctattccgc aaatcgatgt agaaaatagg gaaagccaca
    13081 aagaaaatcg acgaagagta gaacaagagt atatccaaaa ccaaaaagaa tactatgatg
    13141 cgactggtcg agtctacgat ttgcgtacga tcacaactac acacaaagtg gcgttgaaag
    13201 atggaggtgc aagctaatgt ggaaaacgat tctgcgacgt gttttattga tgatcccgca
    13261 agttattatt ttaagtgttc tgatcttctt attagcccaa gcgatgccgg gggatccatt
    13321 taccggtttg atcaatccaa atcaggatcc ggcagtgatc gaacagatgc gacaagcagc
    13381 tggcttgaat gacccatggt atgtccagta cttccgttgg atcggaaatg ctttgcaagg
    13441 cgattttggt caaagtttct tatacaaacg ctctgttgct gacttgatcg gcgaacggat
    13501 catcaacact ttatatctat cgatcttgac agttgtcctt acttatttga ttgctgtgcc
    13561 tctggggatg cttgctggcc ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta
    13621 caacttcttt agttttgctg ttcctttatt tatcttcggc ttgatcatgc tatttgtttt
    13681 tggttatcgt ttaggctggt tcccaacaag tggatcacaa acactaggtt tgcaaggtgg
    13741 atttatcact caatggatag accgtttgaa atatattctg ttaccatcaa ttacccaagc
    13801 atttttggcg actgccgtca caatccaata tctgcgaagt gaagtaatcg attcgaaatc
    13861 gctcgatttt gtcagaacag cccgttccaa aggggtaccg acgaataaag tattcagtcg
    13921 acatattttc cgaaatgcag cgttgccgat ggcttctcaa atggggtatg aaatcacttc
    13981 gttgattgct ggttctgtag ttatcgaaaa gatttttggt tatccgggag tcggaaaact
    14041 atttattgat tccatcggcc aacgggacta taccgtgatc acatccttag tattgatttt
    14101 aggaatcgcg acgctagtgg ggacattact ttcagatatc atcatgagta tcgttgaccc
    14161 gcgtattcgg attcaataaa accaatcaga aaggagaatg gatcgatgag tgaaaaaaac
    14221 aatacagcag ttcaagaaag tattcctcct atggggatac gaatgattgc tcgagaattt
    14281 aaaaaagata aaattgctat tttttcatta ggtttgctag tggttttatt attagtgatc
    14341 tttatcggtg cgatgctgac cgatcaagac aaagtgatgt atgttagtat cttcgacaaa
    14401 tacgctgcac caggaacagt ttcgacacaa ggacaaaagt tcattctggg agcagatgaa
    14461 ggcggacgtg atgtattagg acaattgatc ataggtgcta gaaactctgt tacgatcgga
    14521 tttgcgatca cgatccttac ttcgatcatc ggggttggat tagggatcat ctctggctac
    14581 tacggaggga tcattgacaa catcttgatg cggatcgttg attttatcat gattctaccg
    14641 atcatgctga tcattatcgt cttcgtatcg attatttcca gatacaatgt ctggtccttt
    14701 atattcatca tgagtgcatt ttattgggta gctaaagccc gattattccg aagcaagaca
    14761 ttatctgaag caagaagaga ttatgtcagt gcctcaaaaa caatgggaac caatgatttt
    14821 aaaatcatgt tccgcgaaat catgccgaat ctaagttcat taatcattac taatttgacg
    14881 atgaactttg cggcaaacat cggtatcgag acaactttga ctttcttagg tttcggcttg
    14941 ccggcaaatg tacctagttt aggtacattg atcggttatg caagtaacgg agaagtctta
    15001 gtgaatcggc aatggatctg gttacccgca tcaatcctta ttttagtttt gatgttgagt
    15061 atcaattatg ttggacaagc attcaagcgt tctgcagatg cgcgccaacg gttaggctag
    15121 tatagaagcg aagggggaaa taggaatgaa gaaaacgatt tttggatttg cagcgctgat
    15181 gtctgctgtc gttcttgcag catgcggggg cggaggtaca gatacctctg gaaacaactc
    15241 tggaggcaac ggcaataaca aagaggaatt aacttttcca attgcaacaa aaaataatga
    15301 tgatgcaatc gaagacggcg aattagaagc tgcggtcgca acggacagtc agtttaaagg
    15361 attgttccag tgggagtttt accaagattc atatgatgca gcttttatgg ctccttctca
    15421 tgaaccgcta tttgcaaacg atgacgattt cagaatcaca gacgacggag ctgcaacgct
    15481 tgatctagat gaagatacga aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc
    15541 agatggtgaa gatgtaacgg cggacgatgt gattttccct tacgaaatca ttgggaataa
    15601 agattacact ggtatccgtt atgacgacac tttccgtaac atcgtaggta tggaagaata
    15661 tcatgatgga agtgccgaca caatctccgg aatcaaaaaa atagacgaca aaacagttga
    15721 aatcgaatat aaagaagtaa acccaagcat gcttcaatcg ggtggtggta tttggtcata
    15781 tgcagcacca aaacatacgt tagaaggtgt agcaatcaaa gatatggaat caagcgatca
    15841 agtacgtaaa aatccagtga cttttggccc ttattacatg agtaatatcg ttgcaggaga
    15901 atcagtagaa tacttgccaa acgagcacta ctggaatggt acgcctaaat tgaaaaagat
    15961 cacgatgaaa tctgtaccga tggcaagtgc aactgaagca ttgaactcta aattatatga
    16021 tatgattttc caaatgccaa cagatactta cgatacctat aaagaaattg ctggctatac
    16081 aaacttaggc cgtgaacaaa cttcttatac ttatctagga ttcaagttag gtaaatggaa
    16141 tgcagataca aagagtgtgg aatacaatcc agattcaaaa atggctgata aatcattacg
    16201 tcaagcaatg ggctatgcat tagataataa tgcagtaggt gagcgtttct atgcaggttt
    16261 gagaagcaat gcgacttcat tgattcctcc agtattccag tcataccatg atgatgagat
    16321 gaaaggattc acacaagatc tagacaaagc gaaaaaacta ttagatgatg ctggctacaa
    16381 agatgtggat gacgatggtt taagagaaga caaagatgga aataaactaa caatcaactt
    16441 tgcttcgatg tccggtgggg aaacagcgca accattggca gattactata tccaacagtg
    16501 gaaagaaatc ggtttggatg tccaatacac aacaggtcgt ctgatcgaat tcaattcttt
    16561 ctatgataaa ttagaaaatg atgatcctga aattgatgtt taccaagctg cttggaatac
    16621 aggaactgat ccaaatccaa gtggactatg gggaccaaat gctgcgttca actacactcg
    16681 ttatgaatca gaagaaaata caaaattgat caacgatatc agttcaagca aagcatttga
    16741 tgaagagtac caaaaagaag catatcaaaa atggcaacaa tatgcatttg aagaagcatt
    16801 tgcgattcca acgttgttcc gtaatgaagt attgccagtc aacgaccgtg tcaaagactg
    16861 ggattggtct tatgatgtac caaacccatg ggcaaatgtt tctgttacag cagattcacg
    16921 ttcataatca ctaaaaatga ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt
    16981 ttctgtttat ttctctaata ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac
    17041 ggtgttcaaa gtccagtttc tcggtattaa gtcaaattta tgcaaaaata tggaaagcca
    17101 ttttcacaaa attttttctt catactcgaa actaaacgaa tttttcacaa cttctcgtca
    17161 atcactagga ttattaaagc tttacttacc ttttgaccga accatttcaa atttgacaaa
    17221 aaaccagtat aataaggaag tatcgaaaaa atatccataa aaggaatgtg gaaaatggac
    17281 aaccagctga caaaagaact aaaagaaaac tataacatcg tttttcacga tctaaatttg
    17341 cttgaacaag cttttaccca ttcatcctat gtgaatgagc atcgcaatct gcaattatcg
    17401 gataatgagc gacttgaatt tttaggagat gccgttttag aactgatggt ttctcagtat
    17461 ttattccgtt tatatcctca tttgccagaa ggaaaattga caaaaacacg agctgcgatc
    17521 gttcgcgaag acagcttgtc gaaatttgcc aaagaatgtc actttgatca atatgttttg
    17581 ttgggcaagg gagaagagaa ttcaggagga cgtacgcgac cagcattgct ttgcgaccta
    17641 ttcgaagctt ttttaggcgc gttatatctt gaccagggat tcgacacgac ccatgcattc
    17701 atcgaaaaag tgatttttcc taaagtcaaa gcaggtgctt tttcacatga gatggatcat
    17761 aaaacaaaac tacaagaagt cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg
    17821 atcaatgaag aaggtccagc tcatgaacga gtattttgga tcgaagttta tgtagatgat
    17881 caattgattg gcactggaca aggaaaatcg aaaaaattag cagaacaagc tgcggcggaa
    17941 aatgcattgg cagcactgta aaagtaagtg agggaaagct ttgtatttga aacgaattga
    18001 aatagccggt tttaaatcat ttgcagacaa gacagtcatt gactttgaaa atagcgtcac
    18061 agcagtggtt ggaccaaatg gcagtggaaa aagcaatatt acagaagcta tccgctgggt
    18121 attaggagaa caatctgcta aaagtctgcg aggcggaaaa atgccggata tcatctttgc
    18181 cggttccgat acccgtaagc agctgaatat tgcagaagtc acagtgattt tagacaatag
    18241 tgatcattat ctgccattgg agtacaatga gatcagtgta acccgccgtt atcgacggac
    18301 tggggaaagc gagtttttca tcaataaaca aagctgcaga ttaaaagata tccaagaatt
    18361 gttcttagac tctggattag gaaaagaatc gttctcaatc atttctcaag gaaaagtaga
    18421 agcgattttc agtagtaaac cggaagaccg tcgtgggatc tttgaagaag cagctggtgt
    18481 cttgaaatac aagcagcgga aaaagaaagc agaacagaag ctttttgaaa cagaagacaa
    18541 cctcagccgc gtccaagaca tcatccacga attggaagaa cagttaactc ctttggccgc
    18601 ccagagtgag gcagcaaagg agtttttacg gctaaaagaa acactgactc aaaccgatgt
    18661 ctctttgatg gttgccgaaa tcaagacagc aaaaaaagat tgggacaaca aacaagcaca
    18721 gcttgcgaaa ttcaatctag aattaggcaa actatctgag tcgatccaag aacaagaatc
    18781 cattttggcg aagcagcgta aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca
    18841 gcaagtatta ttagatcttt cggaaaaact gaagcagaca gaaggacaaa aagatgtttt
    18901 gcaggagcgg acaaagcata cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga
    18961 agcacaaaaa aaggttaaac attttgaaaa gcttcaagaa agcttgttga aagaagtagc
    19021 agaaaaagaa acagaaatcc aaaaagctga agcaagtctc ataaaaacac agcaagaatt
    19081 agaaaaatac caaaaatcaa cgaaagaatt actggcagaa ctaagagatc aatatgttga
    19141 tttgatgcaa gagcaagcag ctgtaggaaa cgaactgaag tatctagaac gtcaatatgt
    19201 acaagaaaca gcaaaaagta agcagacgtt agccaagcaa tcggaagtag aggcatcagt
    19261 agaccgattg atcttacaaa aagaagaatt gacacaaaag caggcgcagc taaaatcatc
    19321 tttaactgaa acaaaagaga agttagaaat gatccaacaa aacgggaaaa agttccaaga
    19381 agcattagca aaagaacagc ctaaaatgta tcaattaatg aatcaagtcc agcagctacg
    19441 tgctcgtaag aaaagccttc aagaaatcca agaaaactat tttggttttt accaaggggt
    19501 tcgattggtc ttgcagcata aacagcagct ttcgggtatt gtcggagcag tggctgaact
    19561 gatcgatgtt cctgcagatt tcacgttagc tatcgaaaca gctttaggtg gggcagccca
    19621 gcatgtcatc gttgaaaatg aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca
    19681 aagaggagga agagcaacat tccttccact gacgacaatc aaaccaagac aattaccggc
    19741 tcatatattg acacaagcag cagctgtaga agggtttata gggattgcca gtgaacaagt
    19801 cagttatccc gatcaaatcc aaactgtggt tcaaaacctg ctaggaacga tccttttagc
    19861 caaagactta actagtgcca atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt
    19921 ttccttagaa ggtgatgtga tgaatgccgg aggatccatg acaggtggag cgaataaacg
    19981 aggaaaccaa ggcagtttgt ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt
    20041 tgaagaagca gataaacaac tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga
    20101 aacagctcgt ttagccgaag aacaagaagt tttgcgtaca cgaggcgaac aattacgttt
    20161 tgaagaacaa gaagcgacaa atcagctgca aaacatcata aatgaacttg aacgttttga
    20221 aaaagaaaag caaatctcaa cttttgagac acgtgaactg cagcagttta tcgaagatta
    20281 cgaaaagcaa acgaacgaat tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa
    20341 aatcgacgaa gaaatcaaat cgttaagtca agaaagcgac cagatggaag ccagaagagc
    20401 acaagtacaa agtcaaaaag cacaagaaca agcagatctt gctgtcttga aagagcagtt
    20461 taatcatctg cagatccagc tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg
    20521 tcaagaagcg atagaaaagc agttggcgac attgacagca gacttttctg atcatgaagt
    20581 cacagaagaa agtttggaaa aacaaattaa cgaattatct gctcagcgag aaacattgaa
    20641 agccgagttg gcaaaagcca aagagcagcg agatagaagg caaaaggaga tcgataagct
    20701 tgaagcagtc ttggcagagc gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa
    20761 attagaagtc caaaaagacc gagctgaaat gattttagat aatcatttga cctatctgca
    20821 gtcagaatat cagctgacat ttgagaaagc ttcccaagac tatcaagaaa caaccgatat
    20881 cgaagacagt agaacaaagg tttcatcctt aaaagaacag attgaaaaac tgggaccagt
    20941 aaacttaaat tcaatcgaac aatacgaaca agtcagtgaa agacatacat tcttagcaac
    21001 acagcgagat gatctgttag cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga
    21061 tgatgaagtg cggacaagat tcaaagaagt atttgaagct atccgtcaag aattcaaaat
    21121 tgtctttcca aatatgtttg gcggcggtaa agctgaatta gtactgacag aaccaactga
    21181 tttgctgaaa acaggtattg agattgaagt acaacctcct ggcaaaaaac tgcaaagttt
    21241 aagtttgctt tctggaggag aacgagcact tacagccatc gcgttgctct tttctattat
    21301 ccgtgtctgt cctgttccgt tttgtatctt agatgaggta gaagcagcgt tagatgaagc
    21361 caacgtgaaa cgttttggtc gttacttgag cgatttccaa gatgacacac aatttatcgt
    21421 agtcacacac cgcaaaggaa cgatggtcgc agcagatgta ctgtacggag tgacgatgca
    21481 ggaatccggt gtatcaaaaa tcgtttctgt acggatggaa gatatcaatg aagaggcgaa
    21541 aatcaacgaa acgaaagtgt aggaacgaaa atgatcaaat tagtagcaat cgatttggac
    21601 ggtacattat taaatgaaca aaagctgatt tcagatgaga ataaacaagc attagcacaa
    21661 gcaaaacagc aaggggtcaa gatcgttcta tgtacaggac gccccttagc tgcgatggtg
    21721 cattatctgc aagaattagg actggtagat gaaggtgact acagcatcac tttcaacggc
    21781 ggtttggtac aaaaaaatga tactggtgaa atcatggaaa agaaagtgat ggaagtatca
    21841 gatatccaac gcttatacaa actggctcag caattggatc tgccactaga tgttttgtct
    21901 gacaatgtgg ttttacagct gcctagtgcc ccaaggaagc aatccttgta caatgtgttg
    21961 aataagctgc tgcaattcca accagcggat ataagcgata ttacagatga atttgtgctt
    22021 aataaggcag taatcgctta tgaccaagaa gaattagatc ctaaaatcaa agaaatacca
    22081 gcagaattcc atgaaatgta tgagatcatc aaaacgagaa gtatgctttt ggaatttatg
    22141 ccaaaaggtg ttacaaaagc ttatggtatt tctttattgg caaaagatct tggtttgaaa
    22201 caagaagaaa tcatggcaat cggtgatgaa gaaaatgatt taccgatgat ccaatacgct
    22261 ggactaggcg tagcgatgga gaatgctgtg cctttcgtca aagaagcagc agatttcgtg
    22321 acagccagca atgaagaaaa tggcgtagcc aaagtgatcc aaaaatttgt attagagtag
    22381 gggggagtca aatgggattt tttgataaaa tcaagcgtgc ttttactggc gaagagaaaa
    22441 cagaagaagt tgaagtacag gagaagtacg acaagggttt agaaaagacg cgtaaaacat
    22501 ttggtgaacg gatgaatgag ttgtttgcca attttcgaac agtcgatgaa gacttttttg
    22561 aagaattaga agaaacactt atcggtgcag atgtaggttt tgaaacagcg atacgaatca
    22621 ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa aaaatcggca gaagtccaaa
    22681 atgcgatcat cgagaaactt gtggatctct atgaagcgga aggaatcaat gaagtaaatg
    22741 aactaaatat ccaagaagac ggactaacag ttattttgtt tgtcggtgtt aatggtgtag
    22801 ggaaaacaac aagtattggt aaacttgccc atcagttcaa aaacgaaggt aaaaaagtat
    22861 tgatggcagc agcagatacc ttccgcgctg gagcaatcga ccagttagtt gtctggggcg
    22921 aacgcgctgg tgttgaagtt gttcgtggca atgcaggcgg cgatccagca gctgtcgtat
    22981 tcgatgcttt ggaaagagca aagatagagc aggcagatgt tttactagtt gatactgcag
    23041 gccgactgca aaataaagtg aacttgatga acgaattaga aaaaataaaa cgagtaatcc
    23101 agcgggaatt accagaagca ccccaagaaa cacttcttgt actagatgca acaactggtc
    23161 aaaacgcgat ggttcaagca aaacaattca aagaaacaac agatgtgacc ggtcttgttt
    23221 tgacaaaatt agatggtaca gcaaagggcg gtatcgtact tgctatccgt aatgaattgc
    23281 atttaccagt gaaattggtc ggtcttggtg aaggaatcga tgacttggag ccgtttgatc
    23341 caaatgattt tgtcgttgga ttgttcaaag gattactaaa agaagaataa cccatgtcta
    23401 aaaagcagga gctgtgacaa aagttgcagc tccttgtttg aatgattgtt tttaacatag
    23461 ctgtgttttt gatgccttca tttttatact tatttgtaag cggttctcaa acaatttcgt
    23521 tctttttctt agactttttg attataatta gattcatatg aattggaggt cacttaatga
    23581 tgaattttaa gcaattatta ttgcatgtca atgcgcgccc ttttatcgac caagctcgat
    23641 ttggtataga acgggaagga cagcgggttg atcttgcagg aaatctagca aaaaccgatc
    23701 atccagcaat ctttggcgat cgatcctatc acccctatat ccaaacagat tttagcgaaa
    23761 cacaaacaga gatgatcact cctgttaccg attctattcc cgaattattt cagtatctcg
    23821 ctgctgttta tgatgtgact gctcgttcta taccgaaaga agagatgatc tggccattaa
    23881 gtatgccacc tgccttaccg gaaaaagacg aagcgatcat tattgcaaaa ttaaaaaatt
    23941 tcgaagatgt cttgtatcga cgttatttag caaaagaata cgggaagcga aaacagatgg
    24001 taagcggtat ccatttcaat tttgaatttg gcgatgagtt gctaagaaca ttgttcagcc
    24061 atcaggaaga atttcaagat ttttccgaat tcaaaacaga actttatttg aaaacggcga
    24121 gaaactttat gcgctatcgc tggatgatca cttatttatt cggtgcttca ccaatgagtg
    24181 agaagaacta ctttttagat gaatcacatc cgcaagaacc tgttcgcagt attcgtaaca
    24241 gtgcgctggg ctatacgaat catccaaatg tgaaagtttc ctatgcttct atgaaacagt
    24301 atttagcaga tatcgagcga atgatcgaag aagggaaact ttcggaggag aaagaatttt
    24361 atacaccgct tcgtttccga ggcggaaaga aagtcgcaga tttagcaaca acaggtgttc
    24421 gttatattga attgcggaat atcgatttga atccttatgc aagattgggt atcaatccag
    24481 agcaagttcg gttcttacaa ttattcctga tgtacatgtt atggaaagaa gaaaaagaag
    24541 actgtgacca atgggtagca gaaggtacga ctcgaaataa caaagtagca ctagaacaac
    24601 catcagatca aacagaattt catcaagaag gtagagaaat tcttgaaggg atgaagcaaa
    24661 tgctggttga attggattgg ttggattctc tttatttagt agaggaagca ttgactcaaa
    24721 tggatcatcc tgaacaaacg ttagcagcga aactctatca agaagcacag ctatcgagtc
    24781 agcaagaagt tgctgtggca ttgggacatc aatattataa agaaagccat gaacgtccct
    24841 atcagttggc cggttttcgc gaaatggaac tttctactca gatatttatg ttcgatgcca
    24901 tccaaaaagg tgtccaagtc aaagtattag atgagtcgga tcaatttttg cgtctgcaat
    24961 tccaagacca tgtagaatat gtgaaaaatg caaacatgac aagtaaggac agctatatcg
    25021 tcccacttat catggaaaac aaaacagtaa cgaaaaaagt cttaaaagaa gctgggttcc
    25081 gggtaccagg aggcgcagaa ttttcatcca tggaagaagc ggtaaaagct tatccgagat
    25141 ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa ttacgggtta ggtatcacga
    25201 ttttcaagga aggagctagt ctggaagatt atcaagcagg tttagccatt gcttttcgtg
    25261 aagacagttc ggttttagta gaagaattta tgccgggaac ggaatatcgt ttctttgtga
    25321 tcgacggaga agtccaagcc atcatgttgc gagtccctgc taatgttatt ggtgacggta
    25381 tccgaacagt gaaagaactt gttgaagaaa aaaatagtga ccctttgcgt ggaacgaatc
    25441 atcgtgcacc tttagaattg atccaattag gtgaattaga acaattgatg ttgaaagaac
    25501 aagggttgac gatcgaatca gttccccaag ccaatcaaat tgtttatcta agggagaact
    25561 caaatatcag tacaggtggt gattcgatcg atatgactga tgaattttct gaagcttaca
    25621 aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa gattagtggt atcgatttga
    25681 ttataccaga taaagaaatc gatcctacaa ctgataaaaa agcatatggg atcatcgaag
    25741 caaatttcaa tccagccatg catatgcatg tttatccctt tgcaggaaaa ggcagaagat
    25801 tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt ttaaaagaaa aagcttttga
    25861 cagattgtcc tatttgatgt caatctgatc aaaagctttt ttgcatgtaa aaatttatgt
    25921 gttttttcgt tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt
    25981 tcaactggcg aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt
    26041 actgcttctt ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg
    26101 ttgactacag taaatcggct attgtttttc gttgctagct ggatatattg gttttggaca
    26161 gaaaggggta gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt
    26221 aaaaagtttt ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag
    26281 cgttccctga atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca
    26341 taaagtccct tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt
    26401 cttctctttg tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa
    26461 aagcggttgc ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag
    26521 aaaggtggat tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat
    26581 ttcattgttc atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca
    26641 aagcttacaa atcttgctag tgactcgttt gccacgaacg atttgtttga tccttgtggg
    26701 agcgaccagt agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc
    26761 accaacgact gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt
    26821 cctacctggt gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg
    26881 aacattgctt tttttatccc ttacgcgatt gttcccccag aaaaatcaat tgatgcttcc
    26941 gttgattgga gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca
    27001 attccagttg gtacaaaata tgacttcatg gcttcaagga aattttgcga ctgtgatacg
    27061 aggaaattat gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc
    27121 ttaccgattt acgatcattg gtctgggcga atcgtttgca gtaaatttgg gaatagatta
    27181 ccagaagatg cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat
    27241 catggtagga agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata
    27301 tggagatcaa gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt
    27361 attttgcgat cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat
    27421 cgttggaatc atcggtgggg ctatcttcct ttatcttctg ataagggggc gacgatatgc
    27481 ttaaaaaaaa gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa
    27541 tgtacttgac gatccataca tatggaaact ggcattttgc atgggcattg aggagtaaga
    27601 aaatttttgc atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga
    27661 caattacgca taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc
    27721 ttgtccaaac ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta
    27781 cagttttttt cttattgaac gttgggctga tggtcgggct gagtctattg ttatttttct
    27841 ttcttttgaa aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag
    27901 gtacgctttt tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg
    27961 atttgcttca aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc
    28021 ctgcttcttt gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag
    28081 acgttttcca tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg
    28141 aatttttgat tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc
    28201 cagttacttt tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc
    28261 gtcatggtca gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac
    28321 agctcgcagt agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg
    28381 tcggaggaat ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga
    28441 tttacaaaag gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac
    28501 gattcctgaa aagaagttga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt
    28561 gttgtcgatg atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa
    28621 cgaagtaaag gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc
    28681 gaatgaaata aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta
    28741 tacgaaagga aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct
    28801 tgggttagaa gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg
    28861 tgcgtatatt gcaatggtgc tagcgcaaga cacggattat attttactgg atgaaccttt
    28921 gaacaatcta gatatgaatt ttgcggttca aatcatgcag atcttaaaaa aattagtaca
    28981 agaaatcggg aaaacaattg ttattgttct tcatgatatc aactttgctg caagttatgc
    29041 agacgaaatt attgcaatga aagatgggca gttattcacg caaggaacga cccaagaaat
    29101 gataaaaaaa gatgtactgg acgaattgta cgaaatggac atacgaattt gtgagattga
    29161 aggtcgcaga ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat
    29221 tagtagtagg tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagtgaca
    29281 acaccagtca ggcagataca aagaaaagca gtacgcaagc tgaaacggca ttaacgataa
    29341 aagacagtaa tggcgatcaa atcgaggtgc caaagaatcc tgaaaaagtc gtcgtattcg
    29401 acaatggttc tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag
    29461 ctacaagcag ccttcctgag tatctttcat cctataaaaa agtggaatca gcaggtggga
    29521 tcaaagaacc agacttagaa aaaatcaatc agcttcagcc agacctgatc atcatttctg
    29581 gccggcaaag agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt
    29641 tagatgcgaa aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct
    29701 ttggtaagca agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga
    29761 cgaaaaagaa agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc
    29821 aattatcagc ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg
    29881 aacaagcaga tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg
    29941 tgctggaaaa aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag
    30001 acgacagcaa agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa
    30061 atcagcagat catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat
    30121 caatgaaact gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga
    30181 ttaaactctc atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat
    30241 cctcaaagaa gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt
    30301 ttatttattt gggaaatctt ttttcaacgt gaattcattc tctacatcat acgggttgat
    30361 tctagaatgg ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc
    30421 ctttgatttg aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt
    30481 tattttgtac cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc
    30541 cactagacta gaaaatatga gaagctattt tctgtccagc gttcttatta ctcaaagcaa
    30601 atcgcagttg tcacaatctc ttctaaacga tgttccatct gctgcttctc ggcaataagt
    30661 ccactagact agaaaatatg agaagctatt ttctgtccag cgttcttatt acttaaagca
    30721 aatcgtagtt gtcacaatct cttctaaacg atgttccatc agctgacctt cggtattagc
    30781 tcaaagcact gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt
    30841 gatgatctgc atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttaataattg
    30901 atagaaataa ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt
    30961 cgtttttttt gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac
    31021 aaagagtgtt tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata
    31081 gatgtttttt atatattcct tcatagtgat tcctccaaag aagtcttttt atttgataag
    31141 tttactctac cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag
    31201 ttgttaaagg ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta
    31261 aacaaataac aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg
    31321 gaaagttgtc tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata
    31381 aattgagaac agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct
    31441 atgacatgag gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa
    31501 gaataaatta gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt
    31561 tgcagaaagc caagtcagtt gccaattgga cacggcattt cgattgatca ttgtcaaaaa
    31621 agatacacaa acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat
    31681 caaaatcaaa gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc
    31741 aacttataat agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt
    31801 tgttcgaaaa gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt
    31861 ggacatcaag aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca
    31921 caagatcact cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc
    31981 tgttgtcgaa tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga
    32041 gttggcagat gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa
    32101 tgaacggtat attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga
    32161 ctatcagttt tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg
    32221 gtatgatcgg ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc
    32281 agctgcaaaa aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac
    32341 tcatttcgtt cctaaacggg catttatcat gtatcaatct gctccttatg agcgctggaa
    32401 caaattaaat gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa
    32461 tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca
    32521 cacgatttac agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt
    32581 tgtcttgaaa gaacagctgt tagatagcta tgaacccatg aaattacgtg tcctcttgcg
    32641 tgcccatcat ccggcctttc aagcctatca aacacaacat ttgcaagagg aatttgaaca
    32701 ggcgatgaca atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg
    32761 taaaacataa ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt
    32821 gtggaaaata atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag
    32881 atgcaagcgt ttattctttt tttgaacgat aatcacaaaa aaaaatataa tatacat
//
LOCUS       DAXWLH010000010        32172 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXWLH010000010 DAXWLH010000000
VERSION     DAXWLH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.010"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            140..868
                     /locus_tag="ACGE36_000335"
     CDS             140..868
                     /locus_tag="ACGE36_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFF8382764.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            1024..1341
                     /locus_tag="ACGE36_000336"
     CDS             1024..1341
                     /locus_tag="ACGE36_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8382765.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            1334..1942
                     /locus_tag="ACGE36_000337"
     CDS             1334..1942
                     /locus_tag="ACGE36_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFF8382766.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1997..2788
                     /locus_tag="ACGE36_000338"
     CDS             1997..2788
                     /locus_tag="ACGE36_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFF8382767.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            2926..3414
                     /locus_tag="ACGE36_000339"
     CDS             2926..3414
                     /locus_tag="ACGE36_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFF8382768.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            3465..4337
                     /locus_tag="ACGE36_000340"
     CDS             3465..4337
                     /locus_tag="ACGE36_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8382769.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            4513..4875
                     /locus_tag="ACGE36_000341"
     CDS             4513..4875
                     /locus_tag="ACGE36_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8382770.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            4884..5636
                     /locus_tag="ACGE36_000342"
     CDS             4884..5636
                     /locus_tag="ACGE36_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382771.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            5650..7452
                     /locus_tag="ACGE36_000343"
     CDS             5650..7452
                     /locus_tag="ACGE36_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8382772.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            7729..8769
                     /locus_tag="ACGE36_000344"
     CDS             7729..8769
                     /locus_tag="ACGE36_000344"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8382773.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(8821..10965)
                     /locus_tag="ACGE36_000345"
     CDS             complement(8821..10965)
                     /locus_tag="ACGE36_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8382774.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(11322..12695)
                     /locus_tag="ACGE36_000346"
     CDS             complement(11322..12695)
                     /locus_tag="ACGE36_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8382775.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(12695..13090)
                     /gene="rbsD"
                     /locus_tag="ACGE36_000347"
     CDS             complement(12695..13090)
                     /gene="rbsD"
                     /locus_tag="ACGE36_000347"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFF8382776.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(13118..14035)
                     /gene="rbsK"
                     /locus_tag="ACGE36_000348"
     CDS             complement(13118..14035)
                     /gene="rbsK"
                     /locus_tag="ACGE36_000348"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFF8382777.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(14143..15156)
                     /locus_tag="ACGE36_000349"
     CDS             complement(14143..15156)
                     /locus_tag="ACGE36_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFF8382778.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(15237..15836)
                     /locus_tag="ACGE36_000350"
     CDS             complement(15237..15836)
                     /locus_tag="ACGE36_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8382779.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16022..16723
                     /locus_tag="ACGE36_000351"
     CDS             16022..16723
                     /locus_tag="ACGE36_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382780.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16733..19444
                     /locus_tag="ACGE36_000352"
     CDS             16733..19444
                     /locus_tag="ACGE36_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8382781.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            19895..21523
                     /gene="treC"
                     /locus_tag="ACGE36_000353"
     CDS             19895..21523
                     /gene="treC"
                     /locus_tag="ACGE36_000353"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFF8382782.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(21583..22764)
                     /locus_tag="ACGE36_000354"
     CDS             complement(21583..22764)
                     /locus_tag="ACGE36_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8382783.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            23016..24539
                     /locus_tag="ACGE36_000355"
     CDS             23016..24539
                     /locus_tag="ACGE36_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8382784.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(24608..25335)
                     /locus_tag="ACGE36_000356"
                     /pseudo
     CDS             complement(24608..25335)
                     /locus_tag="ACGE36_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
     gene            complement(25348..26628)
                     /locus_tag="ACGE36_000357"
     CDS             complement(25348..26628)
                     /locus_tag="ACGE36_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8382785.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(26769..27371)
                     /locus_tag="ACGE36_000358"
     CDS             complement(26769..27371)
                     /locus_tag="ACGE36_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8382786.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            27616..27822
                     /locus_tag="ACGE36_000359"
     CDS             27616..27822
                     /locus_tag="ACGE36_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382787.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            28134..28853
                     /locus_tag="ACGE36_000360"
     CDS             28134..28853
                     /locus_tag="ACGE36_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8382788.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(28934..29617)
                     /locus_tag="ACGE36_000361"
     CDS             complement(28934..29617)
                     /locus_tag="ACGE36_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFF8382789.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            29835..32021
                     /locus_tag="ACGE36_000362"
     CDS             29835..32021
                     /locus_tag="ACGE36_000362"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFF8382790.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        10685 a         5639 c         6457 g         9391 t
ORIGIN      
        1 ccaataaaga atactttaac acaaaaaatg aattggtgta atcttctctg tctgttacaa
       61 tctttcttgc agcgaaagca ataattacag aaatttttct gatttttttc gcttcgtaga
      121 aaaagagagg agttttttga tgccaactgg agttttgatc aatgtaggtt ccgttttgtt
      181 tggcggacta attggcggat tagtaggaaa taaattaagt gaacatttta aagcacagct
      241 gacgatggtt tttggtgtgt gttcgatggg gatgggaatc tattccattg cgccgatgaa
      301 aaatatgccg gctgttattt ttgctttagt gataggaaca gcgattggtt tgattgttca
      361 tttaggtaat gggataaata agggggctgc gctgatgcaa gtacctattt ctaaaatctt
      421 ccctagtgaa aagcttggta tgacccacga tgaatttatc agcacattag tgacggtcat
      481 tgttctattt tgtgcaagtg gtacggggat ctatggaagt ttagattctg ggatgacagg
      541 agacagtacg attttgattt ctaaatctgt ccttgatttt ttcacagcag ctatttttgc
      601 gtgtaattta gggtatgtcg tttctgtgat agcaattccc caattcatta ttttttatat
      661 tttgttctta ttggcaaaat ttatttatcc attgactaca ccagacatga tcttagattt
      721 taaagcttgc ggcggattct tgatggtcgc taccggtttt agaatgatca acgtgaaaat
      781 gttcccagta gctgacatga tacctgcaat gatcgttatc atgccactga gctggatgtg
      841 gacaaattgg attatgccac tgttataata aaaatcaaag agaagccatt ttttgctgac
      901 taaaaaggat caaatctttt agtcagtttt tttgtatttg acaaactgat tttgaaacta
      961 tattatatgt tgtataatat agattgaaat aaaatattac acttgagaat agaaggtgac
     1021 gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgctagc agcattaagc
     1081 aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt
     1141 gaatctacac tatatccggt tttgagaaga ttgaaaaaag aaggatatct tgagacctat
     1201 gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt
     1261 catttagaag aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg
     1321 gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc
     1381 tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa
     1441 aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa
     1501 aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag
     1561 acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc
     1621 actagcactg gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat
     1681 ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc
     1741 aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct
     1801 gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct
     1861 tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga
     1921 tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat
     1981 taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg
     2041 cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac
     2101 aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt
     2161 catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat
     2221 aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat
     2281 tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc
     2341 tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa
     2401 agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat
     2461 aactgcgaaa gatgaattag gctcacttcg attagatgag gccaatgtgg ataacttgat
     2521 cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa
     2581 agtcatccta aacggtggca atttatcttt gcatgagatg gaaaacagtg gctatactat
     2641 ccaaacgtct ggtgaatctt atattgaaaa agactaccag ccaataacaa atactttgtt
     2701 agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac
     2761 ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa
     2821 cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa
     2881 aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa
     2941 ggtcaaagag aaaagttaaa aacagaaatg gaaagattac atacttatat cactcaacta
     3001 agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca
     3061 gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag
     3121 aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg
     3181 aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg
     3241 gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa
     3301 ttggattatt taccgcaggc agccggaggg acacaggcaa gcggttggta catcactgaa
     3361 gacaaagagt tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaaa ttaaaaaagt
     3421 aacattagat atttattgaa ggataaagga gcgcaggaag tagcatgaga gaaaaaagag
     3481 cattttatat caatggttat gcagggattg tcggattggt cgtattagct ttagttggat
     3541 tgtttttctt ctatttaggt atgtggcaag cgaaagtttg ggcacttttt ttaagtattt
     3601 ttctttggtt gatcacgtta ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag
     3661 caaaagtaat tttgtttttt ggacaatatt taggtacgat tcgagaaaat ggctttttcc
     3721 ttacgatacc tttggcacaa aaaatgactg tttcgttgaa agttcggaat ttcaatagtt
     3781 ctgttttaaa agtcaatgat ttggatggaa accccatcga aatctcagca gtagtagtct
     3841 ttaaagtcgt tgatacagca aaggcattat tcgatgtagc gtattatcaa gattttgtag
     3901 aaatccaaag tgagacagcc attcggcata tcgctagcca atacccgtat gatacattca
     3961 atgagaatga tttgacgctt cgaggaaaca cgacggcagt gtctgacgag ttgcaaaaag
     4021 agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat cgaaacacgc ttgaaccatt
     4081 tagcctatgc aacagaaatc gctagtgcca tgctccaacg tcaacaagca aaagccattt
     4141 tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat cactcaaatg gccttagagc
     4201 aaatcgaaga tggacaagaa atcaatttca ccgatgatcg gaaagtccaa ttgatcaata
     4261 atttacttgt ctcaatcatt acagacaaag gaacgcaacc tgttatcaat acaggcgaca
     4321 tgaaagaccg taagtgacat actttttgta agataggaag aaggagttta ggacggatta
     4381 ttgtcttgaa ctcttttttt agttgtttta aagaaaatga caaaattgaa agaagaatga
     4441 tagttgattc gaaggcagaa ttgttcgctt ttttctataa tcagtatata aagtaagaga
     4501 gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg ttttcatcgt tgtggtactt
     4561 gcaggggtga aaatcgcaga ttacgtagtt atgggtggag aagaatacta tgtacaaatt
     4621 acgacagatg gtacaaaaac agtttcaaaa tcagactctg gacaagaaac agtcaactat
     4681 agatatatcc tgcctggcta tacgaaagag ggtaaggaaa aagaactgga cttcaaggga
     4741 caattgactc gaccgcttcg taaaggagca tatttaaaaa tcacctggaa caaaaataaa
     4801 ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa
     4861 ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga agtacagaat gtaacaaaag
     4921 tatacggaaa aaacaatgag aaaaagacgc aagcattaag cggcatcagc ttcgatgtag
     4981 aaaaaggcga atttattggg atcatgggcg cttctggttc tggtaagtcg acacttttga
     5041 atattttatc cacattagat aaaccaacag atgggcatat ccgaatcaat caaaaagatg
     5101 tgaccacatt aaaaggaaat caactagcag attttcgtgc gaatgagatt ggctttattt
     5161 tccaagattt caatttatta gaaagcttga cagcacaaga aaatatcgct gttcctcttt
     5221 ctttacaagg cgtcagacca aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt
     5281 tatccatctc tcatattttg gaaagctatc cttctgagat ttccggtgga caaaaacaac
     5341 gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat tctattaggg gatgagccta
     5401 caggtgcgct ggattccaaa agtgcccggg atcttttaga tacgatggac gaattgaata
     5461 caaaagatca tgtatcgatt cttcttgtga cgcatgatcc gttttctgca agttattgcc
     5521 aacgtatttt attcatcaag gatggcggta tccatcaaga agtgaaacgt ggagatcaaa
     5581 gccgtgattc cttctatcga gaaatactat ctattcttgg aaacttggaa cagtaggggg
     5641 agattgacta tgctttggaa attatcactt actgggatca agagccgttt aagagactac
     5701 ttggtgttat tctcaggatt agttatggca tctgctattt tttacatgtt tgaatcaatg
     5761 gcaagcaacg aagctttttt gaaaagtaat acgatcgttg cggctacagt tatcattttc
     5821 cgattcggtt ctgttttact agggatcatc acattcgttt atattttata tgcaaattcc
     5881 tttttgatga cgatgcggca aaaagactat gcaatgttta tgatgctggg agcaaaaggc
     5941 agaaaaatcg cacagatgat tttttctgaa acattcttag tagggatcgc agctacttta
     6001 gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc accgattatt agtggatcag
     6061 ttagatctca atatttcgca ttttacacct tttagtattc acgggttgtt gatcactgtt
     6121 ttgttttttg ctgttttgtt tttattagct gctgttgtca atgctttttc gattgtcaaa
     6181 aaaccgattt tagcattgat tcgtgcggat cagacaccaa caagaatcaa gcagcataaa
     6241 ttcttgtttt tccttgaagt agtactggga attgcttttc taggctgcgg ttactatatg
     6301 atgaaacacg tggggaccta tcaagtcttt ggtatagggg tagccttagt tacgatcgta
     6361 ttaggaactt atttcatttt ccattctgtg atcattttct ttttatcatt actaaagaaa
     6421 tcagaacaga tttcattgaa aaaattgaac aatttcacat tgtcacagtt aaactttcgt
     6481 attcgtgaat atacacaaat gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc
     6541 gctttgacgg taggattagg ttttaaaaat gaaattgcag acttgacaga aaacaattct
     6601 ggatatgatc tagtgatgaa taatcctcgg gcagaagatc agcaaaaaat caaagaactt
     6661 tcaccaacac tttctgcttc ttatactcaa aaagaagatg cgggaacgat ttactataat
     6721 gcaagtgaat ttgcggctca accattgatc atgataaaac atgaagatgg aacaccgccg
     6781 aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg aactgactgt acaagaggaa
     6841 cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca
     6901 gaatttgatc aactgaactt gccacaatcc actttacagc tagttcaggt aaaagacttc
     6961 caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa ataaaatgaa caatccatct
     7021 ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt atgaagtgta taatggtcta
     7081 ttctctggtt ttgaatttat gggattcttc ctaggtttag ctttcttgac catgcttgct
     7141 agctgtttga tgttcaagat cttgtcagga agcaagagtg acattgctcg ttatgaaatg
     7201 cttgaaaaga taggaacacg acgcgggttg cttaaacaat cgatccgtag agaagtaggc
     7261 gtgttattct tagcaccagg aatcttaggt gtagtccatg tcttattcgg attacaaatg
     7321 tttggtctat tcatgcaaaa tccatacaaa gatatttggg ttccgtttac gatattcttc
     7381 gtattgtatt tcatctacta tgtactgacg acatggttat atactggaat cgtattgaaa
     7441 aagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagct
     7501 cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     7561 gttcggaaaa agaggttgtg acaatatttt tgtcacaacc tttttttcac ggtcttttat
     7621 tagaatatag taaatttttt ctcgttactc acggaataag ctgttaagaa aacggtttcc
     7681 atgtatactg gaatcagtgc aaatgaaaga aaagagggtg caaggaacat ggaaatcaat
     7741 gaacaaaatt ttggcgaaaa ctatcacttg attacgatca cgaatcaaaa tgggacatct
     7801 ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc aaacaataat cgataatgaa
     7861 aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat atctagaaaa agacccttat
     7921 atcggtgctt caatcggacg tatggctgga cggattacga atggcacatt caaattagat
     7981 gatgaaacgt tcgaagtaaa aacagacccg gacactggtc attgtttaca tggtggtgca
     8041 cctagctttg aagccaagca gtggcattat acagtcatca atggtgaaaa cgaagcatcg
     8101 gtcattttct atcagactag cccagatatg gaaaacggat ttcctggaac tttagatgta
     8161 gaagtacggt atacgctgac gaacgatgat acatggcgag tgacggtcca agggatcagt
     8221 gaccgcaaaa cagtattcaa tcctacaaat catgtctatt tcaatttaac cggtgatcca
     8281 gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc gattatttgc tacgctcaat
     8341 aaagatacaa cgcctacagg agaagtagtt tcagtaagtg gcacagcatt tgacttccaa
     8401 cgtcctaaaa aattaggtga ggtctttgcc agtgattttc gccaatcaat gatagtgggc
     8461 ggcatcgatc atccattttt gttgaaaaaa caatctggtt tactaaatgc tgcggaattg
     8521 acaagcccag atgaaaaagt atcgatccaa gtaaaaacag atgcttcagg aatcgtgatt
     8581 tttacagcaa attttggaga agatggaccg gaaatgcgcg ggaataaatt agtaaatcat
     8641 ggcggtatca catttgagac tcaagaactg ccaggagcgg agcgacttga aagttttgga
     8701 gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa cggaatacaa aataaatata
     8761 aagaaataga ataaattaaa aagggagtgt gacagtgtca catccccttt ttaatttatt
     8821 ctatttcttt aaaggcagat caagccagaa tgatttgtct aaacttaaca aggttagtac
     8881 aggaacagtt gcagctttaa ctgtacgagt agttgtttct ggtatctgtc ttgccgtaaa
     8941 attgttccac aaatcccaga ttgaagcgac acggaagatg gaagaagcgt tgttgacgcg
     9001 ttttatcgtt gaaaattcta atcctgtgat tggcacgcca tctgttgtcc atttgattgg
     9061 cacatcgaat cgcttagaca tattgaggat ttcagcgttt gcttcttcag ttgttgctgc
     9121 ttcttttttc ggttcatcat attttgtcag gtcgtacgct tcgatgatcg cgttcaattt
     9181 ttgtgaatat tggcgatctg tagcataacg tccttccaaa tatttggttg cttctttgta
     9241 gtcttttgtt tccgacttcc aagttggttt gtagaaatca ggattgccag tcagtccatt
     9301 tttgatcaag tcggcatagt cttccaaact ttccttgtaa gaaggatatt tacggaattg
     9361 tgcgcggatt tggtagcttt gtccggaact atcttgttca agtgtattga aggtcacgct
     9421 gcttccttta tatgatcctt tgataccaaa aaggttatga tttggttcgc ttgataataa
     9481 gctgtttcca gatccacttt caaggatagc ttgagccatc atgactgatg cgtagattcc
     9541 ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt ttcaagaact gatcagttgt
     9601 ttggttttga ctgaaatgga acgagccatc taatccatct aaaatagatg agccgcctgt
     9661 cagcgaacct gaagcagcag gtaaacctgc taagtttact cggacagcaa aatcaggcgt
     9721 gaaatctgaa atagaagtta ctcgaacttt atctcctggt tgaggcgcat ggataaattg
     9781 tccgttccca atatagatac ctacatggtg agtaacaccg gaagaaggtc cccagaagac
     9841 gaggtcgcca ggttgggcct gacttactgg aatctgcgta cctgcatatt gctgttcgcc
     9901 tgtccagcca ccgatataat gaccggttgc tttcatatat acgtaataag tcaaaccaga
     9961 acagtcaaaa gtatttggac cttttgctcc ccatacatat ggttttccaa gatgcttgaa
    10021 tgcttctcga acgattgctt gttggacatt cgttgtattt gtaggcaata cagcatcaat
    10081 tggtgatttt acaacaaaat cagaagaagc agaagatgga ttctctatac cgctttgatt
    10141 tgctgtattt ggacgagatg aagcattgtt cgttccgcca ttcgatgaac cattgtttgg
    10201 atggtttggt tctctctgag tatttccatt gttattcgat ccagatgggt ttgtttgatt
    10261 cgaagaattc ccgctgttcg aattattgtt attattgttt gaattatttg aaccattatt
    10321 attgttgtta ttacccacat ctggtttgtt tggcgtttcc ggatctggtg tactagggac
    10381 ttcttcacta ggatcagttg gtttctctgt tgaagtatcc ggtccagctt taaattctgc
    10441 agtaatagat aaattgttcg tacctactgt aaaggtcttt gcatcataat ttacttcttt
    10501 ggcaccatta acagcgactg aagcaatttg ttcaccttca gctggcgtaa tagtgtaagt
    10561 taccttcgtt tccgctaata aaccttctac cgttccactt tccgtaatat ccaaaggttg
    10621 ctgttcaggg gaatccgctg tcgaagcagt cagttggatc ttaccaatca aagaaggagc
    10681 aattgtaagg gaatatgtct cggctggctt ttcattttct gatggatcag ccggatcaat
    10741 ttcagttggc tcggtcgatc ctgttgttga gttagttgtt ccagttgggt cttctgtaaa
    10801 agaatccgtt gtttcactag tagctgagct ggatgtagtc gtatctgttg tgactgtttg
    10861 atcagctatt tgattatcta cggcaaatac ggtattggtc gacaaaacag acggagcaat
    10921 cagccaagta gcagctagca aacgtatcgt ttttttattt ttcataataa actagtcctt
    10981 ttcctttcat tgattttctg tcttattcag cattcattag acattttttg gaacagataa
    11041 tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg tattctaaat aaaacaattt
    11101 gtaaataagc aacgagcatt cattcgtttt tctttgacgt tcattttatt gcatagacca
    11161 cctttgtttg gggataaaca agtggggata aaaatggtta tgcatatgta aattatacca
    11221 aagtttggaa agtatcacaa aaatttataa aaataatgat cctaaaaaga aaaagactga
    11281 aatatgtgat aaacatctca tacttcagtc tttttcatta actattcata agacattagg
    11341 tcttcatcaa acccgctaac gagagtagtg atcattgcta cgactagact aacagccagt
    11401 cctgctaagt accagaaaaa agtaccggaa atgaatgcag gcaatgtggt cagactacca
    11461 aatacaaaag cgatcgcttt cacttgggca aatcccatta cagctccacc agcagctgca
    11521 ccaatgcaag cagcaacaaa tggttttttc aatgggatcg caattccgta aacagttggt
    11581 tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac cggagatcga acgggttttt
    11641 gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag ccattccaaa attggctgct
    11701 cctgtcaacg gaacgattcg gtcgaaccca taagcagaga tattactgat catgacaggg
    11761 tttacagccc attggatacc aaatattacc aaaatattcc aaccaccagc gatgattgcg
    11821 ccactgatcg gtccgttaat atccatcatc caagtaattg cggcagctaa accttcgcca
    11881 aggtaaacac ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat aactaatgaa
    11941 agtaaaggta caaatactaa ttgcatattt tcgcgaatat attttttgag gaatttttca
    12001 acaaaagaat aaaagaaaat acctaaaatc gccggcatga cagtagacgt atactgcatc
    12061 agtactacag gaatccccag aaaatcagta gctgctttgc cactgtctga aagtaaagag
    12121 gtcagattcg gttcaatcag tgctgcagca atgacagccg caatatatcc attcacttta
    12181 aacgttttgg cagcagaaaa agcaagaaag attggcagaa aataaaagac ggcattcgac
    12241 gcagcagaca aaatatgata agttccgctt gtttcactaa gccagccaat cgtagtcgct
    12301 aaaacaacca gaccttttaa tataccagaa ccggcaaaca gcggaatgat cggcgtaaag
    12361 atttccgata tcaaaccaaa tagtttttcg atcggattta gcttttcttt cggagctgaa
    12421 tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc catgattatt
    12481 tctgcatatc tatcgagtaa aatgaaatac ttaatggaag ggatttgctg ttttaatttt
    12541 tctgcattgg ctactggctg ttttttcaat ttgatacgaa gccgagtcgc acatggaagg
    12601 acagaatcaa tattgtcttt tcctcccaac tgttcaatca gcatatgaat ctgctcggac
    12661 agtaccatct gttcaggatt cattgtcgtt tccattagaa gaccactcca ctcactaata
    12721 taatattggc gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag ttattcaact
    12781 cttttttcat ttcttcatgt gggataaagc taatcggtgt atctggtaac aataatttga
    12841 ttttttccaa tatttccggg ttcattgtct ttatttcttc cgccaattca atcgattgta
    12901 cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa acttggaaga ccgtttgaga
    12961 cagccaaatc tattttttca gtaccaaagg ggacaggcat gcctgcatcc ccgatactta
    13021 atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg attacttttc
    13081 cttttttcat gtttgtttct tcctctcatt gttaatttta gactgctgat tgttctaaag
    13141 cttcgcggta agggatagaa ggttgcgcac cataagactg cacagcaaca gaagaggcta
    13201 gatttccata aaggatcgct tctttcaagt tactaaggtc tggctgaagt gtgctgctta
    13261 atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac ggcatccact ttaaaagcag
    13321 ggacgatacc gtgttctttt tctgtatgat agaaagcgcc ggcactgcct agtgtaatga
    13381 ttactgttcc aatacctaat tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat
    13441 ctgtcactct gattcctgta atgatttctg tttctgtttc atttggtata atgatatctg
    13501 tcttttcaag tagttcagta ggaatcgttt cacgagctgg tgcaggattc aggatcgttg
    13561 tttttcctgc gtcttttgca atagaaaaag ctaggatcgt cgcatctaag ctactttcaa
    13621 actgagcaat aataaagtca ctgtttgtaa tcagttgctt gttttttagg acttcttgtt
    13681 catggaaagc attatttgcc cctgcatgga tcaagatact attttctcct gctgcgtcta
    13741 ccataatcat tgcttgtccg gttgtggcgc cttggatttc agctatccca gatgtatcga
    13801 tgttttcttt tgtcagtaga tctaataact gttgtccttc gctgtcagcc ccaacgcctc
    13861 caataaaaga tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg gcacctttac
    13921 ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg
    13981 ttaaacgaag ggtagtgtcc atattaatac ttccaattac tgtaaccgtg ttcattttac
    14041 aaacctccaa ctatctcatt tattttgatg ggaaaattat tttttaaaac gttttagtaa
    14101 aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca agggtgcagt
    14161 tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt tttctgggaa
    14221 ttggatccga tctaacaata acttggcaga tgtttgtccc aattcgaaga ttggttgtgc
    14281 gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat agccaataat
    14341 gctgtagtct tcagggatgc tttttccagc ttcctctaat ccgcgatata atccaaaagc
    14401 taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat
    14461 ctggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt cgtgagggat
    14521 ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat tttccggtgg
    14581 attttcagga tagacaaggg cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc
    14641 taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt cagatttttt
    14701 ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga ttgcatctgt
    14761 agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga gctcggctag
    14821 atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga cgaaattctg
    14881 cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat tcgtgatatc
    14941 aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag caaaatagtc
    15001 aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt tcggactgaa
    15061 tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc cggaaagttt
    15121 agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat ctaaatcctg
    15181 tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc cagtacttat
    15241 ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc agttttcgga
    15301 aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca agcatcttca
    15361 gcaagcacga tatggtaccc gtgttgaaag gcatctaatg cagtagcata tacaccgttt
    15421 gttgtcgcta caccgcttaa aatgatagtt tcgattccac gccggcgaag ttgaagatca
    15481 agagaagtac caaaaaaagc acttggattg tattttgtga tagtcaacat attatctgta
    15541 tctttcaaag aatccttcaa aagtaaatca gtgaattctg gaggtacaga agcagtcatt
    15601 ccacccatat cagtttgttg actcaaataa gggaaagtgg agatgtctac gttcactaaa
    15661 actttcaagg cagcggtacg tttcaatgtt cttactaaca aatcattgtt tgtcagaata
    15721 gatgctgcag tgtgtggaga gagtgtaccg ttatttacga tctctttttg catgtcgatc
    15781 gcgacaaaag cagtttttga taaatcaaaa atcatatttt cctttaaatc attcatgatg
    15841 ttccttcttt caaataacaa tgttttttat gtaagtaaat cataaatcgt ttatttgtca
    15901 actaaattaa aggattcctt tctttagtct agagaaattc agtggtaaaa taaataagtg
    15961 tgagaaaacg cattcgatgc atagatatag cgagaaatat cgtgaaaatg gaggaatcag
    16021 agtgaagtat gtcgaagtaa aaaatgaata caaacgatac aaaatgggag aaacgaccat
    16081 tacagcaaat gacgatattt ctttttcaat tgaaaaaggc gaattagtga ttatacttgg
    16141 tccaagtgga gcaggaaagt caacgattct taatatttta ggtgggatgg atacacctga
    16201 tgaaggtcag atcattattg atgacacaga tattgcgcaa ttttcagata aacagcttac
    16261 tgcttaccga cgaacagatg tagggttcgt tttccaattc tacaatctcg ttcctaatct
    16321 aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc ttgatcctgt
    16381 agaggtattg aggcaagtgg gcctagctca tcgattgaat aactttccgt cccaattatc
    16441 aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct
    16501 tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt
    16561 attgcaaaac gctagcagac aacacggcaa taccgttttg atcattacac ataattctgc
    16621 tttagcgccc atcgctgata gagtcattca cattaatgat gctaaagtaa ggtctgttga
    16681 attaaatgat catccttcat ccattgatga gattgtatgg tagggggatt ttatgaaaac
    16741 aagaacatat cttaaagcag gttttcgcga aattcggcaa tctaaaggac gattcattgc
    16801 aatcatcctt attgtcatgc taggaacact tttatttgta ggtgtcaaaa ctgctggacc
    16861 tgtcatgcaa aaaacgatgg atcactatgt tggtacagca ggactttctg atttgcaaat
    16921 cgtttctact ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc
    16981 aaaagtagaa acaggaaaac agatctatta ttccaatact ggcaaaaacg aagtgattca
    17041 aatattttct tataacaaaa ataacaagca gaacaagcta caattaactg atggaaaact
    17101 cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat
    17161 cggtgatatt tatcagattg attcagatga attgaaagaa aaagagtata ccatcacggg
    17221 ctttgtccgt tctccattgt tcatcaacaa tttggagaga ggatacgcga acgtcggaaa
    17281 tggtagcgtc gattattttg tctatttacc tgaatccgtt tttaaaacag atatttattc
    17341 tgtcttatat cttgatttta caaacgtaaa gaatttgaat acgtacagca aagcttacaa
    17401 agataaaatg gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc gacccgaaga
    17461 acgcttagaa gagctgaaaa aatcggccaa cgaagaatta gaaccggcac agcaaaaaat
    17521 agcggatggc aaagcacaaa caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca
    17581 gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt
    17641 tcaaacatta tctcaacaag aagctcaact tgctgaacaa gaaaggcaat tagttgagaa
    17701 agaacgtgaa ctggcctctg cagaggaaga attgaatcga aatgcagaaa aaattgaaaa
    17761 tttaactaaa ccatcatacc tatacaatga acgttcagaa aatgtcggtt ttcaagaatt
    17821 tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt tctttttctt
    17881 tattgccgca ttgatcacct ttacgacaat gacacgtatg gtagaagaaa accgcaggga
    17941 aatcgggaca ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa aatatgcaat
    18001 ctatgcaagt ctagcctcag ggataggtat tgttttagga acgatattag gaacgaatct
    18061 tttgccaagg atcatttttg agctgtctaa cgagcggtat gatattggga gtgcgcttat
    18121 tttttatgac tggccgccga ttttacaagc agctgccgca ttcttcattg ccgcatttgg
    18181 cgctgcaatg atcgttttat tcaaagattt aagagaacgg cctgctgcat tgcttcaacc
    18241 gaaagcaccg aagccaggga aacgcatttt tcttgaatac atcactccgc tgtggtcacg
    18301 ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc cgatataagt caagaatgct
    18361 tatggcgatt atcggaattg ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa
    18421 agattctcta agttctgttt cagccaaaca atttggaccg atcattgact atcaagcaat
    18481 cgttacattg gattcatcca cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa
    18541 gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa accgtagaat tgagaaaaaa
    18601 aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg atttggatcg
    18661 ttttatccat ttaactgatc ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc
    18721 tattacgcaa aaagtagcag aactatttga cttatctaca ggagatcata tttcgcttta
    18781 tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg
    18841 tagctttatt tatatgtccc ctgaatatta tgcttcagtt actgatcaat cgatgatgga
    18901 taatgcattt ttgctgcgta cagataagat gacaacaaaa gaagaagaaa tgctttccga
    18961 gaaactgctt gccacagacg ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac
    19021 gcaagaagaa tcaatggcga acttagactc ggttgtctta atctttgtcg tattatcagg
    19081 actgctggct tttatcgtct tgtataatct aactaatatc aatatttccg agcgtgtacg
    19141 cgaattatct acaataaaag tccttggatt ttttgataaa gaagtcacga tgtacattgt
    19201 tcgagaaaat atcatcttta ctcttttagg aatcatcgga ggatttggtg taggatatat
    19261 attgactgac ttcatcttgc aacaagcgtc tatggagaat gtcatctttc cattagtgat
    19321 cacatggtgg gcttatgtct tatcagcagg attaactatc gtatttacgg ttattgtgat
    19381 gattgttacc catttcaaat taaagcatat cgatatgatt gatgcgttga aatcgaatga
    19441 ataaaaacag aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggcaa
    19501 aatagttttt catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
    19561 catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc aaaaagaata ctcaaataaa
    19621 attagctcct catgttttac atttgagaag actgattgcc gtagataaaa gcttttgaac
    19681 accatttata gaagaaacaa gaatattttg ctattttaaa caagaggata gacaaataca
    19741 tgtctgatct cttgtttttt attgggcaac agacctctta gaatgtataa agataaattt
    19801 tacaaacgct ttcggttcag ttataataat aaatgataac ttgtatatat aagttgtggt
    19861 aggtaataaa tcagttgaat ggaggtaaat atccatgaca tttaaagaca aagtcattta
    19921 ccaaatctat cccaaatctt acaaagacag taatggtgat ggaattggag atttaaaagg
    19981 aatcaaagaa aaactcccgt atctgaatga tctaggcatc gacatgattt ggctaaaccc
    20041 tatttatcca agccctcaaa aagacaacgg ttatgatatc tctgattaca cagccatcga
    20101 tcctatcttt ggtacgatgg aggaattcga acagctaata gccgaagcaa agaaataccg
    20161 gatagaaatc atgctcgata tggtgctgaa tcacgtttcg acagaacatg aatggttcaa
    20221 aaaagcacta gcaggggacc agcattatca ggacttcttc attttaagag aagagccaac
    20281 ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca tttggagata cggggaaata
    20341 ttatttgcac ttatatgatc ggacacaagc tgacttgaac tggcggaacc aagcagtaag
    20401 agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa ggggtaaaag gattccgttt
    20461 cgatgttatc aatgttatcg gtaaagacga acaattgaaa aataataaag aaaatgatgg
    20521 caaacctgaa tatacggaca aaccaatcac ccatacgtat ctaaaagaat tgaaccaagc
    20581 aacgtttggg acggattcca ctatcatcac agtcggtgaa atgagctcta cgacgatcga
    20641 aaattgtatc ttgtatacag aacctagtag agaagaatta tcaatggtct ttaatttcca
    20701 tcatttgaaa gtcgattatg aaaatgggaa taaatggtct actcctccat ttgattttgc
    20761 agaattaaag cgactatttc atacatgggg agaaaagatg agtgagggta acggctggaa
    20821 tgctctattt tttaataatc atgatcagcc acgtgcgctc aatcgatttg tagctatcga
    20881 tcagtatcga aaacaaggag cagagatgct agcaaccatg atccatttga accgtgggac
    20941 gccctatatt tatatgggag aagaaatcgg catgatcgac ccagactatt catcgatttc
    21001 cgactatatt gacgtagaaa gtttgaatgc ttataagtta ctccttgaac aaggattcac
    21061 agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc gataactctc gaacacccat
    21121 gcaatggtca gacacagaac aagcaggatt tactacaggg aaaccttggt taaaagttgg
    21181 aggcaagcta gaagagatta atgtagaaaa agaactatct tctgaagatt ctattttgtc
    21241 ctattacaag aaattgattc ggctgagaaa aacatatcca atcgttgcac aaggagatta
    21301 tcatgcttat gaagcaaatc atccggaagt ttatggcttt cttcgccaat ttgaaggaca
    21361 gcagctgctt gtattaacaa atttctatga taaagataca aggatcgcca tcccagaagg
    21421 attcacagaa gccaatattc ttatcgataa ttattccgat actgttcttc atcaaagtct
    21481 ggagctaaaa ccataccagg caattgcttt gctgaaaaat taaaacaaaa acacaaaacg
    21541 tcgttcctac gagctattct cgtaagatcg acgttttgtg ttttatttaa aataatcttc
    21601 ttcagaaaat gaagagtttt catactctcc tttatcgcca taaaatttat tgtagcgttg
    21661 tttgaattta cggaaaataa aagtaacgag taatttgact aacgtataga taggaatacc
    21721 aaaaataacg cccacaattc ccattagatc acccatgaca agtaaaacga ttaagatcgt
    21781 aatcggatgg atctgtaaac gatcacccat tactcgagga atcaccaagt caccgtggag
    21841 aagttgtacg gcaaaccaga caatgacaaa tttgacagcc ataaaagtag agtcttggaa
    21901 tgcaatgatc aaacctggaa agaaggcgat aaacggccca atatatggaa tgatacataa
    21961 caacccagca gaaatagcta aaacactagc aaattctaat ccgatcagta agaaacaaac
    22021 ccagtagact gctccaagga taaatgaagc aatgatttgc ccctttagaa atgaacccaa
    22081 ttgttgatta gcaatctttg tcaaattttt aaaatctgta cgaaatgctg gtgggacaat
    22141 cgctaaaact gactgataaa atttttgagg atctctcaaa aggaaaaaag cgacaattgg
    22201 accagtaaat aaagtaataa aaatagctgt aacagttgaa aaaatgttgc tgatccgttg
    22261 tgcgccactt tcccaataat ttccaataaa tccagctata tcagacgtca cgtcgtcaat
    22321 agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt ttctccaaaa attgtcggag
    22381 actctcttga aaatcttgaa acaaatctgg caaactactg atcaatgtct ttgcctgttc
    22441 ttcaagaaaa ggaataagcc atatgcttcc aagccccaat aaaaataaaa taatcacata
    22501 aacgataatg actgacaagt tcctcgaaag gtgcttgctc aaacgtttga tgactggttc
    22561 caaaagataa tacaagatca gtgcaaacaa taatgggggc aagatagtag cgataatgat
    22621 tccgacaggc ttgaatataa acgagatctg atggaaaaaa taaatgatca gcgcaagtaa
    22681 gataactatt cctaaaacat agtaagaggt ttttcctcct aaaaagcgga tcaaacgatt
    22741 cgttgtatct gtttcttttt tcactctttt tcctccttta tctataactt cattatagac
    22801 aaaagcgggt ataaaaggaa aatgatagag gttgattgca aaaatatcgt caatcattca
    22861 tataaacttt ctcatcaatt aaaataagaa aacgcttgac agttctttta accatatgat
    22921 aaattaaatc ctgttgctaa aacgtaacta ttacggtttg cgaaaagcga atcggaacat
    22981 tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa acgattgata ggtatattgg
    23041 ccatgctggt tttagcaggt atgttaactg cttgcggggc aagtgggaaa gccgaggaca
    23101 gtaaagaaaa attgagcgtg atgacgacct tttatcccat gtatgatttt acaaaagcaa
    23161 tcgtcgggga tgaaggagaa gtagagttat tgattccagc agggactgat tcccacgatt
    23221 atgaaccttc tgctaaagac atggcaaaga tccaagatac agatatcttt gtttataatg
    23281 atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac attacaagaa ggcaatgtcc
    23341 atacgataaa agcgacagaa ggtatgcttt tacttccggg aagtgaagaa ggacatgatc
    23401 atgaccatga acacggggaa gaaggccata cacatgagct agacccacat gtatggctag
    23461 ctccaagttt agcaatcaag caggtagcaa acatacgtga tcagctgatt gaagcttacc
    23521 cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta taccgagaaa cttcaagcac
    23581 tccatcagct ttatcaagaa acctttaagc aagcaaaaca aagatctttt gtcacgcaac
    23641 atgctgcatt caactatcta gcgttagaat atggattgaa tcaagtatcg attgctggtc
    23701 tttcttctag tgaagaaccg tctgcggcga gaattgctga actgaaacac tttgttaaag
    23761 aacatggaat caactatatt tattttgaag aaaacgccaa agatagtatt gccagaacgt
    23821 tagcaaacga agcaggtgta tctttagaag tattgaatcc tttggaagga ttgacgaatg
    23881 aacaaataga aaatggagaa aactatcttt ctatcatgga agcaaacctc gaagcgttga
    23941 aaaaaacgac agagacagaa aacccgctag aagatacttt gacccccaaa aaagaaaaga
    24001 gcgtctacaa cggttatttt gaagacgagg atgtacaaga tcgctctctt tcagattggg
    24061 caggaacttg gcaaacagta gatacgtatg tagaagatgg gacttttgat cctgtttttg
    24121 aatataaaga aaaattgaat caggataaga cagcacaaga atataaagaa tattatacga
    24181 agggctatca aacagatatt gcttcaatca agatcagtga gaaagagatg gcatttacgt
    24241 ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt agggaaagaa atcttgacct
    24301 acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc agaagataag gaaagtggcg
    24361 cttttagata tattcaattc agtgatcaca cgattgcacc ggctgactcc gaacattttc
    24421 atttatatgc aggaaatgaa agtcaagaag tattgctgaa agaaatggag aattggccaa
    24481 cattttatcc agatacaatg gatggacgtg aaattgcaca ggaaatgctt ttgcactgat
    24541 aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt tagtcggtcc aaactattcg
    24601 gttcttttta tgaaatcaat ttctttaatc cacggataga ggctgggatg gtctcgccgt
    24661 ttgccaacgt gacttctctg gttttttatt aatcgaccgc acattttgtg tattgataac
    24721 ataggattta tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc
    24781 atagaattgt aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga
    24841 agtagaaaaa tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat
    24901 tttgaagata tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat
    24961 atgctcgaca ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc
    25021 gatgttgctt tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt
    25081 tcctaaagga tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc
    25141 ttctagttcg atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt
    25201 gagaagttct tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat
    25261 gattctattt ttgatcaccg ttgataaaac ttctaattgt tttggatcgt cttcacaaat
    25321 ataaatggga atcatatcat tactccttta ttttatttgt atataaatcg tttgagaaaa
    25381 acgctggttt tcaattgatg tttcaaggaa aagataagga ttggtctttg tcaattcatc
    25441 caagataaac aagccaaggc cttcgttttt tggatttgtt tttgttgaga accctttttg
    25501 tttcaactgg tttaacggta atgtatttgt tgttgtattg atcacacgaa tcatcacgga
    25561 atgattcttc ttgagtatgg tcaattcgat ttttttcttc tttgcagcac tagctccttc
    25621 aatcgcattg tccaagataa taccaagcat acgtacaagg tttaccgtat gtttcgtatc
    25681 cagttcgatt ggctcaggaa aatgaagctg gacgatcaac cctttttcct gtgccatcat
    25741 gagttttaat gacagtaaac tacggatctc aggaactttc atattatcca acatacttag
    25801 attggccgga aaaaggttca tcatctggcg tgtaggtaat atcgtttgat cataataaga
    25861 ccgtaatcct tctaagtcgc cttctgaaat atagctgtga agagaaaaaa gcagattttg
    25921 gtaatcatgt ttgaattctc tcaagtcttg atagtttttt tctagatctt tgatataatc
    25981 ctgttgcatc ctgacttgtt gctctaataa ctgatcagct aatttcttat tagagatttt
    26041 ccaataataa accaagatga tcaggccaac acacagatat agaatgaaaa aactcgtatt
    26101 caaagctaag acatcaggtg tttcaccaga aaaacgggta taaaaaataa tgatatagta
    26161 tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg cggtccacag tgtgaaacca
    26221 agtctctaac gattttttta aacaaaaagc taataaagca gataaaaaag cggataagac
    26281 aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc gaagatttca aaacaaaaaa
    26341 atcgccgatg cttgttaagt gatctcctaa aagcgacgtc aataacccaa aaggaagcgc
    26401 gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat
    26461 agcaattaga gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga
    26521 aatcagaatg actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat
    26581 acaaattgtg taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc
    26641 ccattgattt ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga
    26701 ttttgaattc agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag
    26761 ttaattgttt aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt
    26821 gtaataggaa gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag
    26881 taagaagctg agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc
    26941 tttaataatg ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca
    27001 tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata
    27061 gtcaattgca gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc
    27121 agacaaacga attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa
    27181 gaaagaaagg tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg
    27241 accgctagtt ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac
    27301 accatatctt cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact
    27361 ttatctttca tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg
    27421 tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc
    27481 ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta
    27541 tgacaagttt gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt
    27601 gatggaggga ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt
    27661 aaatctgatt tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt
    27721 gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac
    27781 tggcagttag ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga
    27841 gagcataaag aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa
    27901 aacagctttt catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact
    27961 gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag
    28021 gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt
    28081 ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga
    28141 taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc
    28201 ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc
    28261 cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca
    28321 aacgcttttt ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca
    28381 tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag
    28441 aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc
    28501 cacaaaagaa accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga
    28561 gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct
    28621 actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca
    28681 aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa
    28741 tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga
    28801 tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa
    28861 actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt
    28921 tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg
    28981 tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa
    29041 acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta
    29101 gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt
    29161 tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga
    29221 ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg
    29281 cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata
    29341 gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg
    29401 ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc
    29461 attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa
    29521 aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa
    29581 gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat
    29641 atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac
    29701 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    29761 agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga
    29821 aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct
    29881 gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta
    29941 cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag
    30001 aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca
    30061 atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag
    30121 gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa
    30181 aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac
    30241 taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc
    30301 atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc
    30361 aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac
    30421 agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt
    30481 ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta
    30541 aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg
    30601 gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg
    30661 tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa
    30721 aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg
    30781 tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga
    30841 gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc
    30901 ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag
    30961 caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac
    31021 aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt
    31081 ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca
    31141 tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata
    31201 ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc
    31261 aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc
    31321 aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat
    31381 gcgatcaata cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga
    31441 taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta
    31501 aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta
    31561 tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg
    31621 ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa
    31681 aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc
    31741 acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt
    31801 tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac
    31861 cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa
    31921 atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat
    31981 ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca
    32041 gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt
    32101 gaaatttcag cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga
    32161 ggtgtgacaa ga
//
LOCUS       DAXWLH010000011        31795 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXWLH010000011 DAXWLH010000000
VERSION     DAXWLH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.011"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            490..1215
                     /locus_tag="ACGE36_000363"
     CDS             490..1215
                     /locus_tag="ACGE36_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFF8382791.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            1205..1879
                     /locus_tag="ACGE36_000364"
     CDS             1205..1879
                     /locus_tag="ACGE36_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFF8382792.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(2167..2451)
                     /locus_tag="ACGE36_000365"
     CDS             complement(2167..2451)
                     /locus_tag="ACGE36_000365"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFF8382793.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2745..4052)
                     /locus_tag="ACGE36_000366"
     CDS             complement(2745..4052)
                     /locus_tag="ACGE36_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8382794.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4541..5647
                     /locus_tag="ACGE36_000367"
     CDS             4541..5647
                     /locus_tag="ACGE36_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382795.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            6238..6438
                     /locus_tag="ACGE36_000368"
     CDS             6238..6438
                     /locus_tag="ACGE36_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8382796.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            6585..9374
                     /locus_tag="ACGE36_000369"
     CDS             6585..9374
                     /locus_tag="ACGE36_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFF8382797.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            9421..9948
                     /locus_tag="ACGE36_000370"
     CDS             9421..9948
                     /locus_tag="ACGE36_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFF8382798.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            9969..11159
                     /locus_tag="ACGE36_000371"
     CDS             9969..11159
                     /locus_tag="ACGE36_000371"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8382799.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            11199..12497
                     /gene="asnS"
                     /locus_tag="ACGE36_000372"
     CDS             11199..12497
                     /gene="asnS"
                     /locus_tag="ACGE36_000372"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFF8382800.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            13431..14105
                     /locus_tag="ACGE36_000373"
     CDS             13431..14105
                     /locus_tag="ACGE36_000373"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8382801.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            14102..14443
                     /locus_tag="ACGE36_000374"
     CDS             14102..14443
                     /locus_tag="ACGE36_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382802.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            14473..14832
                     /locus_tag="ACGE36_000375"
     CDS             14473..14832
                     /locus_tag="ACGE36_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFF8382803.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            15356..17437
                     /locus_tag="ACGE36_000376"
     CDS             15356..17437
                     /locus_tag="ACGE36_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8382804.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            17748..19214
                     /locus_tag="ACGE36_000377"
     CDS             17748..19214
                     /locus_tag="ACGE36_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8382805.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            19499..19997
                     /locus_tag="ACGE36_000378"
                     /pseudo
     CDS             19499..19997
                     /locus_tag="ACGE36_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            20071..20511
                     /locus_tag="ACGE36_000379"
     CDS             20071..20511
                     /locus_tag="ACGE36_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFF8382806.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            20524..20733
                     /gene="copZ"
                     /locus_tag="ACGE36_000380"
     CDS             20524..20733
                     /gene="copZ"
                     /locus_tag="ACGE36_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFF8382807.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            20733..22919
                     /locus_tag="ACGE36_000381"
     CDS             20733..22919
                     /locus_tag="ACGE36_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8382808.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            22939..25110
                     /gene="copB"
                     /locus_tag="ACGE36_000382"
     CDS             22939..25110
                     /gene="copB"
                     /locus_tag="ACGE36_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFF8382809.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(25222..27393)
                     /locus_tag="ACGE36_000383"
                     /pseudo
     CDS             complement(25222..27393)
                     /locus_tag="ACGE36_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(27383..28135)
                     /locus_tag="ACGE36_000384"
     CDS             complement(27383..28135)
                     /locus_tag="ACGE36_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFF8382810.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(28132..28455)
                     /locus_tag="ACGE36_000385"
     CDS             complement(28132..28455)
                     /locus_tag="ACGE36_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382811.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(28469..29344)
                     /locus_tag="ACGE36_000386"
     CDS             complement(28469..29344)
                     /locus_tag="ACGE36_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8382812.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            29671..30960
                     /locus_tag="ACGE36_000387"
     CDS             29671..30960
                     /locus_tag="ACGE36_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382813.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            31034..31603
                     /locus_tag="ACGE36_000388"
     CDS             31034..31603
                     /locus_tag="ACGE36_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8382814.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
BASE COUNT        10538 a         5547 c         6622 g         9088 t
ORIGIN      
        1 aaaagatagc atcatttagt agtaaatata tcgaatatag aaaagttagt tactaaatag
       61 tcaatcagtt ttttcacgac gtttctatta taacgaatta tgttgaaaaa agcttttatc
      121 gaaaacataa atcttcaaat taacagattt tttagacctc gatcatgatg aagacttttg
      181 tagatacaaa taactgtaac attgaaaatg aatgcgaaaa cgatattttt ttttattgga
      241 aaatatcggt caagcaataa accagttgca taataaaatg ttaacggcaa caaaatctgt
      301 ggaaggaatg aaccggatga tttgatggaa aaaacaagat agacaagaag tatacatagt
      361 aaaacgtgga acaagtaggc cagaatgaaa aatcagtttt tatccactcg cttttctata
      421 cattcactgt cacaaatata atcgaaagta attctatttt gagataaagt attttatagg
      481 agatagtata tgaacaaaaa aagatttaaa cgtattgaaa gaaaatttgc attaacttat
      541 gaccaagcta agtatttgaa aacactattt ccaaaacagg tagttcggga taaatatagt
      601 gatcatttta tttcttcctt gtattttgat acaaacgatc atcacttttt aaaagaaatc
      661 actacagaaa agtcctatcg agaaaaattt agaatcagaa aatatggaga tcctcaggac
      721 atatcgccta tctacttaga gataaaaaga aagtttcaag gaatgacaga taaacgtaga
      781 ataaaattag cgcatcatga gctcgatgat atgttggaaa atatcgaatt ggcagaaacc
      841 acattcagta atttagaaga agagtatatt tttaatgaaa tacagtggtt gttcagaagg
      901 ttggcattgg agcccaaagt atatatctat tatttaagag aagcatttgt aaatagagat
      961 tcagaagatt tgcgaatcac ttttgatcaa aatatccatt accgtctaat gaacttaacg
     1021 acctgggaac actgtacaga agacaatctg gtagcgccag aaatcgatgt gctgatggaa
     1081 gtaaaaacga caggagaaat acctgaatgg ttacaggtag gattggatag gatgggtaaa
     1141 aaagagataa aattttctaa atataaacaa gtctataaaa gatatatcca agaggacccg
     1201 ctctatgttc aatagtgtct taaaaaatga gccttctcaa ctcacactag atgatttctt
     1261 tttatgtatg gtggtgtcga ttttgctagg cattctgatt gcttacaccc atatgtatca
     1321 aaacaactgt acaaaaaact tcatgtttgc cttgattggg ttacctactt tagtacatac
     1381 cgtcattttg ttgatcaacg gtaacttagg cacggggatc gctgttctag gcgcatttaa
     1441 cttagtgcga tttcggtctg ctcaagggaa ttcaagagat attttcagta ttttttgggc
     1501 aacgtcgatt ggtttagcca cagggacggg atatattgtc tatgcactat tcgtgtctag
     1561 tatagttgct gctatccaag tcatcatctt cacgcttcca atcaaaaata aacatacagt
     1621 ttttaatact gaaaaaacga tcaagctaaa agtttctaaa tccttgtttc tttcgagtat
     1681 tattgaagaa aaaattgcag aatatacaga aaactgcaat ttaaaaaaag taaaaatcaa
     1741 taaagaaact tgttccttac actatatagt aaaagtaaaa acgagtatgc ttgaaaaaga
     1801 attggttcga acgatcatgg atcatccaga agtcatttcg atcactgtag gtatggataa
     1861 atcgaataaa ttattgtgat aaccaagaaa gtacttaaca atgaagtaag accccagttc
     1921 caaagtttag cggggacact tcaataaagt actttctttt ttgtcttcag caactactgg
     1981 taaaactgag agcaagcttt cgtgagagta cagcgataaa gatcaacctc cctttttatc
     2041 tcgagcaatt tccttgcaag gctgtttcgt tgatcttaaa cgagttagca acagtcaaag
     2101 atgcgatggc cataaaaaaa ttgcattaag ttggctggta tctgatagct gggaatctaa
     2161 ggaagactat ccattctgtt tcttaaattc cgctagttga tagatatcag ccaattgttc
     2221 cagatgatca acatcaaaga taaccgaaag atgtttcctt ttattcccat ttgcttgaag
     2281 agtttttatc ttctctttgg ttccttcact caatgagagt gtgactaatt tctccctcgt
     2341 tttttctggg aaatgaaagg atactttgaa aaatccgtcc caaatagaaa gccagaagat
     2401 cggttttact ttctgagagt gggacaaaag taagaaacac ttttgtctca ttctttaaaa
     2461 acgaataaac ggtgggagca gaagcaactc cttcggaaat aagccaaaat tcacaaaaat
     2521 ttgaaaagca attttcgtga atttcttctt atttctcgga gttaaacact tctgtcccaa
     2581 cctcttgtat tcaccttttg atagccaagc ttttccatca ttataaaacc gcaaatttca
     2641 aaaaaaaacg aacaatacaa aaagcgtatt gctcgttttt ctcttcagtt tctcacacct
     2701 aaacgacttt tgtctcgacc tctttttggt gactattcat gatgttaaaa taaattactg
     2761 aaaaaatctg tgacatggcg ccaaccaatg acaaaaatat tagctttttc aacagtagaa
     2821 gtggtaatga tgtctgtcga aggctcttgg tttgcttcca aatagcctaa tttgtcatca
     2881 gctaattgga tcgttgctga gccaactgta gtgcctttgt tgataggagc ggtcacttgg
     2941 tcatccgtta ctttcttttt atcaattgtt ggagtaatcg ttaattttcc agtatccatt
     3001 ccattttcga cccagacttt tacagggctt tttagcgcaa cattgacgga ggtttctttt
     3061 ccatctttca ctttgatgtc ttttaggctt gggatgcttg catttgcttt tccaagttct
     3121 ttttctgacc agttgtcata acaataatcc atcaatcgtg atgtttcatt gaatcgggca
     3181 cttggatttt cttcatggtt atctgcattt aaaacgactg tgatgattcg tcggccatct
     3241 ttgttgatcg ttccgacaaa gcatgcaccc gcaaaatcag ttgtgccagt ttttaatcca
     3301 tcgacacctt cgtgatagtt tacaaaacca ggtagcatcc agttccaatt gaccatttca
     3361 accggtgact gtgtgttttc gccgaatgtt tgatttgtgg tactcgtgat ttttaagact
     3421 tcaggataat ctaagatgag gtgacgagca acaatagcga catctttcgc agacagttga
     3481 ttttcatctg tttcaccagt attctcaggt cgattctcac ctaggtaaga attgtttaaa
     3541 cctgatgcat tgacgatcgt tgcatctttg ataccccaat cttttagttg tttcttcatt
     3601 agctcaagaa atttactttc actgcctgca actttttcag ctaaagcaac catggaagca
     3661 ttggctgatt ggatgaaagc tgagtcaaca agttctttca ctgtataatc attttcttta
     3721 tgcaaaggaa cattcgatag atccggcacg acacttaatt tttctgcata atccgaaatg
     3781 gctaccttat cttcccaaga taatttaccc tctttgattt gttcaagtac gatatataga
     3841 ccaatgatct ttgtgatgga agcaattccc atcgtttttt cgccatcctg atcataaagg
     3901 atttttccac tttctgcatc tacggcaaag gcagctttgg catttatttt aaatgcgtcg
     3961 tctgcttgaa cagttgcgct aggcaagaaa gaagtgatac ataagataag acttgctaat
     4021 agtacaataa aacgggtatt ttttttgaac atctttgttc tcctcatttt taaattgatt
     4081 cttacaactt agaatattat cagagagaac aaaaggtttc aatggttctg aaaaaaacct
     4141 tataaagaac aagtcttatt aatgaagaat catagaaagt tatcttttgg gttcattgac
     4201 acgaactttg actggctgaa cttcgggttc ggttaggcgt gatgctgcaa aaagggcgcc
     4261 tgctgagagt gcgaccaaac taaaaatcat gaataattgc ataagatcac ctcgttcttt
     4321 tttgtttttt tatagtatag caagagagtt tgaagctact gttaaggaaa tgagcatata
     4381 attgatgaaa gtatgaaaaa aatatgaagt aggacaacac aactaaaaat cgatcaggta
     4441 tttctctggt agggattcgt tttattgtat tgataaaatg ggatgtggta gactttattt
     4501 acagatacaa agaaatgatt tttttaggag gggtacgttt atggcaaaaa aagaaaaaga
     4561 attattatca caattgactt cagaaggtac caaaggaccg tttgtcacga ttatgttaaa
     4621 cacccatgtt gcgcatcaag acgtagaaaa agatcaaatc aaattcaaga attttgcaaa
     4681 ggaagcgaaa aagcgctttg aaaagaaata ctctgagcat gattggtctc cattccaaga
     4741 aaaaattact agtttgttgg acgatccgtc tttctggcgt agtgccacaa cgagtgtagc
     4801 tgtcatagta tcaccagaac aagtgtttat ccaccgtttg ggtattcctg tagatgacca
     4861 gtattatgtg agtgatctac cttatttatt agcgatcatc aaaaatggac atttcaacta
     4921 ttcatactat ttattaggat tgaatcgtga ttcgatgaaa ttgtacgaag taacgaataa
     4981 acaagtcaaa gaagtggctt tgcctgaaga tgcaccaaga gatgtcgtca ctgctttagg
     5041 atctgaacta acaggaggcg ggttgaacta cgcatcaaat ggtagtgaag gtacgacgtt
     5101 ccatggagtc aatcctaaag atgaagaagt cgaaattgat tgggtcaatt attatcaagc
     5161 agtcgatgac tatttgaaag atacatttga aaatccagag aaatggccta tcttcttatt
     5221 tgcattacca gaaaatcaga cgatgttcaa aaaagtggcg aagaaccctt attatcgttc
     5281 cgatgttgcg atctcagctt cacctgcaca actttcatta aaagaaattg aagcaggcgc
     5341 aaaaaaacta tcgcaagaat tagctgtcaa agaagcaagt gactacaata agttgcttga
     5401 taaaaagttc attgatcaac tcgttgatat tataccggca gcaaaagaag ggaaaatatc
     5461 ccatttattc attgctacat ctaatttagc agatggtttt ggcgaagatc cagaaacaga
     5521 atatgaccgc cgtcaggtgc tgaatgaact agcagataat gtgatcacga acggtggtca
     5581 ggtctttatc cttgaacaaa aagatgcacc agacgaaaag agtttagttg cgatcttacg
     5641 ttattgagtg aaaactacca aagtagatga atggtcaggc gttgatagaa gtgtatagct
     5701 ccgagagaag gagtttcctc tgtttcagcc gttggattca tcttgagcga gagacaaaag
     5761 ctagcttttg tctctcgctc attatttttg tgctttagtc ccattggctt tgcgagggga
     5821 agatactttt ctcaaaaaaa cgaaccttgc agttgagagg gggatagatg cgcatactaa
     5881 tttttatcag ttaaaaaatg atgtggatca ttagagaatt ccaaagttcg ttcatccttt
     5941 ttcagataat aaattgaaat tttttgcaaa agtgaaaata gcaaatagaa attttctgaa
     6001 tcttataggg gtgatttcga gaaaacgttc gtttatttgc aaatatatct tctaaataag
     6061 agaataaata caaaaaaata aggaaaaact ctggcaattt tgtaaacggt atgatatagt
     6121 aagaatgtag tttttgttcg gtaatctgtg gtagcttgtt tcaaataaac atcttccaga
     6181 tatacaccat tcaagtaatt gcaaaatatg tgttgataca caaggaggat ttttattatg
     6241 gaaacaggta cagtaaaatg gtttaactca gaaaaaggat ttggattcat cactgcagaa
     6301 aacggaaacg atgtattcgt tcatttctca gctatccaag gcgacggatt caaaacttta
     6361 gaagaaggcc aagcagtaac tttcgacgtt gaagaaggtc aacgcggacc tcaagcaaca
     6421 aacgttacta aagcttaatt tacctatatt aaaaataaaa gtccagacat ttatgtgtct
     6481 gggctttttt gtcttttttt ccttaaaaag cgatgagagt atttttctgc actttgtagt
     6541 ataatttcaa aagaacatta gaggcaggag aagaggaaga tattttgaaa ccaagaactt
     6601 atgcagtcgt tgatatcgag acgactggga cgaatccaaa agaagatcgg atcatccaat
     6661 ttggctgcgt catgattgaa gctggtcgga tcacttcacg tttttcgatc gatattcatc
     6721 cagggcggaa gatttcaaaa caaatccagc atttaacagg gatcacgaat caacgcgtcc
     6781 aaaaagcgcc ttattttgaa gatgtcgccc aaacgattta caatcttttg gcagacacca
     6841 tatttgtcgc gcataatatt tattttgatt ataatttttt gaaccatgaa ctcatgcgat
     6901 gcggacttcc tagtttgaag ataccgggca tcgataccgt agaacttgca caaattttct
     6961 tgccgacgga accaagtttt cgcttagctg atttgtcaga aagccttggc tttattcatg
     7021 agaatcctca tcaggcggat agtgatgccg aagtgacagg tcagcttctt ttactgatcg
     7081 aagaacgaat gaggaaattg ccgatcatca cattagagca gattgcacga ttaagtagcc
     7141 atacaggaat ggatacgagt cgtttcattt accacgtgct tgaagaaatg aaagaaaacc
     7201 cggagcccct tgatctctca ttagaaatca tcgatgggct agctttgcag aaaaaagaag
     7261 tggagctatt cacatccgtc cattatggcg agcggacata tccaagaaaa aaacaagcaa
     7321 aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga acagaacagg ctgatgaatg
     7381 ctgtctacga ccatttcaca aaagatgaat caaaagattt gatgatcgaa gcagctacag
     7441 gaatgggcaa aacgattggt tatctgttgc ctttgagtta tttggctaca cctgaaaaac
     7501 cagctgtcat cagcaccgtt tcgcttgttt tgcagcagca gatcatggaa aaagacattc
     7561 cattactgaa tcaattgcta gatcagccga tacaacctgt gattatcaaa agctatcgcc
     7621 actatattga tttacagcgt tttaaaggta cacttgtgga accgccggga caaaaacaat
     7681 atgctttgta ccaaatggct gtcttagttt ggctaacaca aacgaagacc ggtgatttgg
     7741 atgaattaca tttaaccaat ctgaaccatg cattctttgc agatgtcgcg cacagaggca
     7801 ctgggttttt agcaaggaat cagtcatttt acaaacaaga ctttgtgcgt ttcttgcaaa
     7861 agaaaatcaa acaaagcaat tttttgatag tcaaccatgc atttttagta caagaaacac
     7921 agcgaaaaga accattgctt ccagctagtc cttatttgtt gattgatgaa gtgcatcatt
     7981 tgccggaaat tgctgaaaaa gtaaatgaca aacgactaag tattaactac ttccataaac
     8041 aaattcagca actggaagag ccgaatcagt tatttgaccg catacaaagt tatttgccga
     8101 aggaacatga agccttgcgc tggttgtcga tttatcgaga agaactctct gcactatgcg
     8161 aagcgcagat ttgcgtggca gagggattaa tgaacttagt aagcagcaag caacctaacg
     8221 gacaatatcc agaagaagtc atgatgacaa aagaaatgtt ggatcagctg ccactggaat
     8281 ctggaacagc aatggatcat ctgcaacttt tttatcaaga gatccaacaa ctacaatcac
     8341 agatgcgctt gacttttgag attatccaag aaagttggac caaccagatg cggatcgatt
     8401 tagcaagttt atatagctta tttgatcaat tagacaagca atacttgctg atggaccaat
     8461 ggctagaaga ctggcgagat catttgatcc attggttcgt tcccgggaat acagcacagc
     8521 agatgatttt tcatctcctt gattttcaag cagctatgtt gccgtcaact gtgtggtatc
     8581 ctcgatatga ccatattctt tatattggag gaacactgaa gatcggacct aatcgacagt
     8641 atctctctaa acgacttggc attgaagcag cacctcttaa agtgattgct tctccttatg
     8701 attatgagaa acaggcaaga atctatgtgc cgaaagaagc acctgcagtc catcaagtca
     8761 gcaatcaagc atatatcgat tatttagaag cagcactcac aggattgatt gaacaagaag
     8821 atcggccgat ccttgtttta ttcacttctc atgatgtttt gcaaaaggta tatcagaaaa
     8881 tgcatttgtc tatgctagaa aaaggacgtg aaattcttgc tcaaggattt ggcgggagtc
     8941 gggaaaaact tttgaaacgc ttcattttgt cagaaaaaag cgtcttattt ggtgctgaca
     9001 gcttttggga aggcgtagat ttacctggag atgctttgca gattgttgta gtcacacgtt
     9061 tacccttcga caacccgtta cgtccgatgg tcaaagctag gaatgcttat ctggaatcag
     9121 aggggatcaa tcctttttat caggaatccg tgcctaaagc agctttgaag ttacgccaag
     9181 cattaggtcg tttgataagg acggagacag atcgtggcgt attacttatt ttggatcgga
     9241 gattagtcac tgcaaaatat ggtgcacgaa tcatccatgc attaccgaaa aaagtaccga
     9301 taaaagagct agctatagat gaaatctatc aggatatcca tgaattctta aagaaaaatg
     9361 aagaaggaag ttaaaggtgc cgtctttttt catcattaaa atctgctata atgaaaagga
     9421 atgaagataa aggagcgacg acccttgaag aaaaaacaga caaaggaaaa aacgattaat
     9481 cgcgtgttgc ttggcttgat cgtattttta ttagcggtca tcgtgatcag ctcgattttt
     9541 tacttgcgtt ccagtcgtcc ggcggtccag gcgaaaaaag aagcagtaga aatcgcaaag
     9601 aaatatgcaa atctcgagga agccgatcat ttttactggt ttactcgaaa gaaaacgtac
     9661 ttttcattga cgggtacgaa tgataaagga aaagaaatcg ccgttatcat tcctaaatct
     9721 ggtgaaaaag taaaagtatt ggatcaaaca aagggactga ctgaaaacga agcaaaacag
     9781 acaattgctg cggcccatcc tgaaatccaa atcgaaaaag ctgctttagg gatgtatgat
     9841 gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg ggaaactgaa ttattacctg
     9901 attggctttg aatctggaga tgaagtcaag acaatcaagg gaatttaaaa ggtaaaggaa
     9961 tgaatgggat ggagttatca aaaagaacaa aacgattaga accgtctgtc actttggcag
    10021 ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga tgtgctgagt ttgactgtgg
    10081 gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc agcaatcgaa gcaatccgca
    10141 atgggaaagc tagctattat acaccaacag caggaatccc agagcttcga caggcaatcg
    10201 ttgattatct gaagaaaaac tatggacttg tctatgaacc ttcacaaaca gtcgtaacag
    10261 acggagcgaa atttgctttg tatacccttt tccaaacgat ccttgacccg caagatgaag
    10321 tcatcattcc tgtgccttac tgggtcagtt acggtgagca agtaaaactg gcagaaggac
    10381 ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa agttactgtt gagcagctag
    10441 aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa ttctccatcg aatccgactg
    10501 gaatgattta tagcaagaat gaactgcagg caatcggtga atgggcagta aaaaatgata
    10561 ttttgatagt tgctgatgat atttatggcc gtttagttta taatggcaat gagtttactc
    10621 caattgcaac gatttcagaa gcaatcaaaa atcagaccat catcatcaat ggcgtgtcta
    10681 aaacatatgc gatgacaggc tggcgcattg gttatgcggt ggggaattct gagatcatca
    10741 atggaatgat tgctgttgct tcccagtcaa ccagtaatcc gacagcagtc agtcaatatg
    10801 cagctgttga agcattgaaa ggcgagcaag atacagtgga agaaatgcgg aacgcctttg
    10861 aagaacgcct gaatacgtta tatccgcttg tggcagaact gccgggtgtt tctcttgaaa
    10921 agccacaagg agctttttat ctttttccta atgtaaaaga aacgctacgc atgtgtaaat
    10981 atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta gcgcttgtga
    11041 ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgccact gatcgcgcta
    11101 cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt caaaattaat
    11161 cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt caaatcatcg
    11221 attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca aataagcgat
    11281 caagcggaaa aatcgcattt ttgcaattaa gagacggaac agcctttttc caaggtgtag
    11341 ttgtaaaaag cgaagtgccg gaagaagttt tccaaacagc taaaaatcta caccaagaaa
    11401 catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt ggttatgaaa
    11461 tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc acaccaaaag
    11521 aacacggaac agacttcttg atggatcatc gtcatttatg gctgcgttct tcaagacaac
    11581 atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc tttaatcaac
    11641 atcattttgt caaaatcgat ccgccgatct tgactggttc tgcaccagaa ggcactacgg
    11701 atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt ggccaattat
    11761 acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg actttccgtg
    11821 ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa ccagaaatgg
    11881 ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca ttcttagtac
    11941 aaaatgtttt agatcattgc gaatacgcat tagatgtact aggacgagac aaagaagtct
    12001 taaaaactta tacaaaattg ccattccctc gtattactta tgatgatgcg atcgaattac
    12061 tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca cctcatgaaa
    12121 cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca aaagccatca
    12181 agccattcta catgaaacca catccaacaa gagaagacgt ggtgatctgt gcagatatga
    12241 tcgcaccaga aggttacggt gaaattattg gtggaagcga acgggcaaca gactatgatt
    12301 tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca tggtatcttg
    12361 atctacgtaa atacggttct gttccgcact ctggttttgg tctaggcctt gaacgaacag
    12421 tcacttggat tgctggtatc gaccatgtgc gtgaagctat tccattccca cgtttgttga
    12481 accgaattta tccataagag gttgtgaaat cagtgccttg acctgagtat tagagaaaca
    12541 aaacggaaaa atagcttctc atattttact gttttttgag ctaatatcga gggtcagctg
    12601 attgaacacc gtttatatca agaggttgtg cgtctgccgt tcagctccga gtcacaagga
    12661 gtaatttcaa aaaaacattt tttcatgttt gatgactatt gtgacttgtg accaaggagt
    12721 tggccggaga gcaacgttta tttaaaaaaa agaactgcga actctcgcag ttcttttttt
    12781 ttaaatacgg gataaaggaa gtttccagca gataagagaa gaaagcaaaa gaaattgcta
    12841 tttgattcac aaggttctat aacgaaatcg caatcaatca aacatataca aagaaggtta
    12901 ttcaaatgga aatctgattt gatgatgctt ctgctgttga aataaagcag catctagcac
    12961 ctgaaaacaa actaatagta acattcgaag atggagtagg tccgtatttt cagcatgcaa
    13021 tgatccatat gtaagttcaa ttcacccttg aaatcatcgg accagataaa gttatggcca
    13081 atctatccca agcaatctag aagagatatt agtaaaggaa catttactgg aagtcttaga
    13141 tgagcctata aatatatgtt ctgatggcgt catcaagtgg tctgattgac ggaagcttag
    13201 gtgtgatcga tctctctgga ccaaacaatg aaggaatcaa ggggaatcca gcacgttaag
    13261 aaaaaaatag cataaaaaac tagacacttg atgagcgttc attcaattgt ttgaagggac
    13321 taggctggtg tcatttttta tacatatatt ttctctttcg tcttgatagg gcttatggga
    13381 taatataagg aagaaaatca tcgttttatt caaaaaggag tttttgacaa atgaaaaaac
    13441 gtatattatt tatcggtgac agcattacag acgcaggaag agaccgagca gaagcaactg
    13501 atctaggtaa aggttatcca cttttgattg ccggaaaact aatctcccaa tatccaacga
    13561 tggaatttga atgcatgaac cgtgggatag gaggagacaa aattgcagat ctgcaagtac
    13621 gctggcgaga agattgttta gctttacaac ctgactatgt atcgattttg atcggaatca
    13681 atgatacctg gcataatact agcgatcagg ctatattcgg aactgagaaa tctgctcagc
    13741 aatttgaatc cgtttatcga aaattactaa atgaacttca aaaagcggga atctgcaaca
    13801 tcatcttgat ggagccgttt gtacttccta taaccgacga ccgtcatggc tggcgtaaag
    13861 acttagaccc taaaatccaa attgttcgta aacttgctaa ggaatatggc gcattgctgg
    13921 ttcctttaga tggcatattc aatgctttag ggattgctta tggtttttcg aaatatacaa
    13981 gagaagatgg ggtccaccca acgattggcg gacatgagat cattgcacaa agctggatag
    14041 atgcaattgc cgcagtcgat ttttttagtg gagataaaaa tgagcgaaag aaaagtgaga
    14101 aatgatggaa atacggtggg cagtaaaaga agatctacct caaatcaatc agctgattac
    14161 tgatcatcca gaatcattcg taaaagcaaa aacggcagct gacctaaatg ataagaataa
    14221 actattgctc gttgctgtaa caaaagaaaa acggattatc ggttttgcgc aagcggacgt
    14281 gcaacagtca gaagaacggg tgggtcagcg accatcagga aaactaatcc aacacacatt
    14341 agtaacaaaa gaaaatacgg aagaaatcca aaaaaaattt gcagaatatt tgatgctgga
    14401 agctaaaaaa agaagaataa aatcaatttt tgcccagttt taatcaaaaa cagtaataga
    14461 aggagaaata caatgagttg tttaggaaat gtcatctggt ttatattcgg cggactaatc
    14521 ggtggtctta gctggttagc agcggggata ctttggtgta tcacgatcat aggtatccca
    14581 atcggtatgc aatgttttaa gatcgccttc ctttcttttg cgccttttgg caaagaagta
    14641 atttatcacc agtctggcat gtcattgatc gttaatatca tctggctgat cgtttcaggc
    14701 ctaccaattg ctctcgcaca tgtcgtcagc ggcatccttc tatgtatcac gatcataggg
    14761 ataccttttg ccaagcaatc tttcaaactt gctcgattgg cgttgatgcc attcggtgca
    14821 gaaattgtat aaaagagaat aataaaggtt agataaagca tagagtcagg tgggatacat
    14881 gtatttcacc tgactctatg cgttttcctg tataagttct tccatgtgac ttttacgtct
    14941 tttatttata tggggttact gtttatggaa agttgaaata attttcatta aagaactctt
    15001 tctatatttt tgaaagcgta gtggagcttc gtatatctgg gaaggacatt ttgcagggat
    15061 tgaaaaaaga tattgtccta tcgaaaaatt aatgaattta gacaaattgc cagcaacagg
    15121 cttcaaactt agttgctatc ctgtcaagat caagaaggca agtgcgggtt ggataagagc
    15181 agtggcaatg atcgaagaga aaaagaaaga ataagccaaa acatctgtat ggaagaaatt
    15241 ttccgatgca gatgtttttc attttttctt tcggatacct tgacatatga ataaccattc
    15301 atttataatt agttatgaag agatattcat atgttgaggc gaggaggtca ttaaaatgaa
    15361 aagttacaga ttagaaggtt tagattgtgc gaactgtgca atgaaaatcg aaaaaggagt
    15421 tcagaaaatc aatggagtca aggaagcaac ggtaaacttc acttctggaa aactaacgat
    15481 tgacgcggag gaagatcatt tggctacgat tgaacaagaa acaaaaaaag tagtgaagga
    15541 attagaacca gatgtcaaag taacagaaat agataaggaa aaagtatctg aacatggaaa
    15601 tgaaaaagag agaaatacgc tgttccggat tttattttcc cttgcaggta tcgctttatt
    15661 gctattattt gattttaatg aactaattcg cttgataggg tatttattga tttatctgct
    15721 gataggatat gatgtagtga agaaggcagt gatgaatatc gtcaaaggaa aaatctttga
    15781 tgagaatttt cttatgtctg ttgctacgat tggcgctatg ttgatcggtg aatatccaga
    15841 agccgtagca gtgatgctat tttatcaaat cggcgagtat ttccaaggat tggcagtcag
    15901 tcattcaaga aaatcgattc gcgaattgat ggcgatccgt ccagaaacag ctcacgtgca
    15961 gacggcggaa ggattgatga ccgtgaaccc tgaagacgtg ctgatcggtc agtttgtact
    16021 tgtgaagcct ggtgaacgtg taccgttaga tggaacgatc atcgaaggag aatcattagt
    16081 cgatacgtca gcacttacag gagaatcagt ccctaaatct gtatatgttg gtgaaacagt
    16141 attaagcgga tttatcaata aaaataaacc atttttagtt caagtagaaa aatcttatga
    16201 aaattctact atcagcaaat tattagaatt agtagaaaat gccagcagta aaaaagcacc
    16261 agctgaaaat ttcattacta aattcgcgcg ctattacaca ccagtcgttg taggattggc
    16321 cgttttatta gctgttttac ctccacttgt tgtttctgga gcggcgtttt ctgaatggat
    16381 ctatcgagca ttgacatttt tagtgatctc atgtccatgt gcattagtga tttccgtgcc
    16441 acttagtttc tttggcggta tcggaggagc aagtaaaatc ggtgtattag taaaaggttc
    16501 aaattacttg gagttgcttg cgcaaaccga gacagtggta ttcgataaaa ccggcacatt
    16561 aacgaaaggc gacttttcga tccagacgat cgatacaaaa atcgatccaa agatattcat
    16621 gcaatatatt gcgagtgcag aacagttttc tacacatccg attgctcaat ctgttttaga
    16681 aggctacgca ggtccgcttt taccaacagc taatatccag gaatttgcag gtgaagggat
    16741 cttagctgaa gttgatggca aacaagtatt agtaggtaat cacaaattga tggaaagatt
    16801 tgaaatctct tttccttcaa gtcaagaaat cggtacattg ctttatctag caatcgatca
    16861 aagttacagt ggttatcttg ttattgcaga taccttgaaa gaagatgccg tagacgcatt
    16921 ggttcaatta aaacaggctg gtgtgaaaaa cacagtcatg ttgacgggcg actcgaaaaa
    16981 aattgctgat catatcggga agcaagtagg cgtggacaag atatatagcg aattgcttcc
    17041 agaagataaa gtgcagcggt tagaggaaat cttacaaagt aacaataaaa agaccgcatt
    17101 tgtcggagat ggaatcaacg atgcaccggt gctagcaaga gcagatgtag gaatcgcaat
    17161 gggtggattg ggaagcgatg ctgcaattga agcagcagat gtagtcatca tgaatgatca
    17221 accatcgaag attgcagaag caatccattt agcaaagaaa acattgaaaa tcgtcaaaca
    17281 aaacattgtc tttgctattg ggataaaaat tttggtactt tccttaggtg cttttggttt
    17341 tgcatcaatg ggagatgcag tatttgcaga tgttggggtg acagtattag ctgttttgaa
    17401 tgccatgcga agcttgcatg taaaaaataa acgttgatca atgaagagga cgaatgaacg
    17461 tcctcttttt tttatatcct tttcttaatg aaaaatgtaa agaaaataga taggattata
    17521 aaataactaa tgtttttaat tttgcagaaa attcaaaata gcttgaaatt caataaggtc
    17581 aatgaaataa aagaaaataa cttatttttt tgatggaaaa aatatgaaaa aacgatgtgt
    17641 attaaataaa ataatacaca cataaaataa cactgtcttg taagtttgtt aaaaattgaa
    17701 tatgattaca tctggataac aaatttttat cataaaggag agaacgaatg aacaacaaac
    17761 aattacgctg gtttactgtt gcgttaattg catttaacat ggtttggggc atggggaatg
    17821 tagttaataa ctatgctcag caaggagtat cagttgtcac ctcttggcta ttgattttgg
    17881 ccatttattt tattccatac gcattgatcg ttggacaatt aggatcagcc ttcaaggata
    17941 gtaaaggagg cgtcagttca tgggtcgaaa atacgacatc taataaacga ctggcatact
    18001 atgccgcttg gacttactgg gtcgtccata ttccttattt ggcacaaaag cctcaagcaa
    18061 ttctgattgc tggtggttgg gcagtccaag gaaatggaaa tatcgtaaat acgatgtcag
    18121 tacaaatggt agcagttatt tcacttatta tatttcttgc ctttttatat ttatcaacaa
    18181 aaggactttc tactttgaaa gtgattggcg gactggccgg aacagcgatg ttcgtcatgt
    18241 cgatcttatt tattctttta gctgtaacgg caccgttcat caatccaacg atggaatttg
    18301 ccacaccaca tatgaatcaa gtgaaaacat atattccaac atttgatttc agttatttca
    18361 caaccgtttc gatgctagta tttgcagttg gaggggcgga aaaaatatcc ccttatgtca
    18421 actctacaag aaatcctgca aaggaatttc ctaaagggat gatcttctta gcagcaatgg
    18481 tagggatttg tgctgtatta ggctcagtcg caatgggtat gctgtttgct agcgataata
    18541 tccctaaaga cttgatggca aatggggcat acagtgcttt tcaaatttta ggtaattact
    18601 atggtatcgg taactcattg atgatcattt acgcattgac aaacatgatt ggacaaatct
    18661 ccgcattagc cttttcaatt gatgcaccac taaaaatctt gttagcagat gcggatccag
    18721 aatttgtacc aaactggcta cgtaaacgta atcgtaaagg tacattggtg aatggttatc
    18781 ttctgacagg tatactagta ggaatcatta ttttgttgcc gatctttggt atcaaagata
    18841 tgaatgaatt agtaaaatgg ttgacgaact tgaactcagt tgtcatgcca atgcgttacc
    18901 tatgggtgtt cttggcatat atgatgttga ataaagctta taagaaattc aattctgaat
    18961 ataaatttgt taaaaatcca aaaatcggtt ttatctttgg tgcttggtgc tttgccttca
    19021 ctgcgtttgc atgtatccta ggaatggtgc caaaagtaga ttatactgct gatcctaaag
    19081 catggtggtt ccaattattc acgaatatca tcacaccaat tgtcttgatc ttattaggta
    19141 tgatcttgcc agcaattgct cgtcgagaca aaaacaaaga aattactgaa ccagaaacag
    19201 tggttcagcc ttaaaataga aatacttgtt acaagaggtt gtgacagaag ctacttctgg
    19261 aacaccgtga atcagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
    19321 acaaaaatag ctcctcatat tttgccgttt tttgagctaa tgctgaaggt cagctgattg
    19381 aacaccattt attcgaaaca agggactttg acacgacttt tgtcacagtc ccttgttttt
    19441 ttgcggcgtt ataagtggta tgatcgaacc aaattccaaa gagaagtgag ggagaagaat
    19501 gaagatcatc gtgattggac aaagtgggtc gggaaaatcg tccctcgcaa ggaagatcaa
    19561 agaaatcacc ggctttccgc attcaggcaa aaaagtggtt tttgcaagaa cagcaatcgt
    19621 ttatagtatc taatccgtca tggatcgttg aaggaaatta tacttctacg ttagaggaaa
    19681 ggattaaaga agcagataag atcatttggc ttaaagtgcc gcgttatctc gccatctacc
    19741 gagtagtttg gcgttctcta aaaagaaaga taaacaaaaa atctcggcca gatatgcctg
    19801 aatcattttc ggagaaacta aacaggaaat acttagaatt tttatctttt atctggcatt
    19861 ttgaaggaaa caatgaacca aaaatacagc agctgatcca agtagctaat gcaagaaata
    19921 aactgatcat ccttaaaaca agacgagaga aacaagaatt tttaactaaa ttgaaaatag
    19981 agagcaaaaa taattgacag tttttgttta caagcgtaat ctgtattcat aagctaatga
    20041 ttacatttgt aaacgaaagg tgtgaaataa atggaaaaag tcaaaacgcc agtaaagatc
    20101 agtgattcag agtgggagat catgcgtgtt atttggacac ttggacagac gacagcccaa
    20161 gaaatcacgc agatcttagc tgattcgatg gattggaaac cagcaacgat caaaactttg
    20221 ctgggacgtt tggtaaaaaa agaagtgatc tggacagaac aaaaaggaaa aaaattcatt
    20281 tatcatccag ctgtctcgga gatggaaaca gttcgcagtg cgacggaaaa tcttttttcc
    20341 catatctgtg caaaaaggat tggagagacg attgcggatc tagtagaaga agctactttg
    20401 actcaagaag atatcaatga gattttgaaa caactggaac aaaagcaacc agtagatacg
    20461 atcgaatgta actgtatccc aggtcaatgt gaatgtaaac aacatcaata ggaggaagaa
    20521 aaaatgaaac aacaattttc aatcgaaggt atgagctgca atcattgtgc agcaagagta
    20581 gaagaaacag taagcgcact tgacggcgtg caaaaagtga aagtaaattt gaaaaaagca
    20641 aacggtacgg tcaagtttga tgaaacaaaa gttcaatccg aaaagatttg tcaagcaatc
    20701 aatggattgg gttataaagc agaggtgatt tgatggcaac agcagaaacc aaaatggata
    20761 cgttcgtcat tacaggtatg acttgtgcaa attgttctgc taggatcgaa aaagagctga
    20821 acggacaacc tggtgtggtc aatgccacag tcaacttggc cactgaaaaa gcaagtgtca
    20881 aatatgaagg gacgaccact gaaaaattaa tccaaagcgt agaaaacatc ggttatggtg
    20941 caattttgta cgatgaagcc cacaaacaaa aaattgcaga agaaaagcaa gcgtatttga
    21001 aaaaaatgct attcgatctt atattgagta cggttttgac tttgccgttg atgttgtcaa
    21061 tgattgctat gatgttagga agccatgcgg cgattgttca cttttttcat tttccaatcg
    21121 tccagttagt gctgtctgct cctgtccaat tttatgtcgg tgctcgtttt tataaaggtg
    21181 cgtaccatgc aatcaagacg aaagcaccaa atatggatgt attggtagca attggaacat
    21241 cggctgcctt tgcattgagc atttacaacg gatttttccg tggacacccc caagatcttt
    21301 attttgaaag cagcagtatg atcatcacgc tgattttgct cggcaaatat ttggaacata
    21361 cagcaaaaac gaaaacaggt aatgcaatca aacaattgat gtctcttcaa acaaaaaccg
    21421 cccaagtgat aagaaacggg aaagaagaaa cgttagcgat tgaagaagta gtcgttggtg
    21481 atcaactagt catccgtcct ggtgagcaga ttccagcaga tggtcgaatt atttcaggaa
    21541 gcagtgcgat agatgaaagt atgcttactg gtgaaaatct tcctgtagaa aagaatccgg
    21601 atgacacgct ttttggcggg acaatcaata cgaatggtct gctacacatg gaagtcacac
    21661 aagtcggcaa gcagacagta ttagcgcaaa tcattcaaat ggtggaagat gctcaaggga
    21721 gcaaagcccc aatccaaaaa atcgcagatc ggatctcagg gatttttgtt ccgatcgtat
    21781 tagtcatcgc gtttatcacg ctcatagcta caggtttgat cactggtgat tggcaactcg
    21841 cattgatcca tagtgtctct gtattagtca ttgcgtgtcc atgtgcatta ggtttagcga
    21901 ccccaacagc tattatggta ggaacgggtg ttggtgcacg aaatggaatt ttaattaaag
    21961 gcggagaagc attagaagca gctgctcact tagacagcat tgttttggat aaaactggaa
    22021 cgattacaga aggaaagccc aaagttacag atctagtcgg ttcaaaagaa gtcctatcta
    22081 ttttttatac tttagaacaa gcttctgaac atccactagg taaagcaatc gtggaatacg
    22141 gaaaactgca agaagcagca acatacgata tgattgattt tacagcccac ccaggtgctg
    22201 ggatcagcgg tacgattaat ggagtacgct attttgcggg tacacgtaaa cgattaattg
    22261 aattgaacct atcatttgat gaataccaag aacacgcgtt agaattagaa cagcagggaa
    22321 aaacagtcat gtttttagct gatgaaaagc aagtgatcgg gtggattgcc gtggctgatc
    22381 aaatcaaact agaagccaaa caagcaatca agcagctcca gaataaagga ttagatgttt
    22441 ttatgctgac aggagacaac aaactggcag ccgaaacgat tggtaaacaa gttggtattg
    22501 atccaaagca tatctttgct gaagtacttc cagaggataa agcagcctac gtagaaaaac
    22561 ttcagaaaga tggcaaaaaa gtcggtatgg caggcgatgg aatcaatgat gcaccggcct
    22621 tagcattagc tgatgtcggt atggcaatgg gaagtggaac ggacattgcg atggaaacag
    22681 ccgatgtgac gctgatgaat agcagtttgg catccattgc acaaacaatc gagttgtcta
    22741 ggttgacatt gagaaaaatc aaacaaaatc ttttctgggc ttttgtctat aatacgattg
    22801 gtattccttt tgccgcacta ggctttttga atccaatcat tgcaggagga gcgatggcgt
    22861 ttagttccgt cagtgtattg ttgaactccc tgagcttaaa tcgacacatg aaaaagtaag
    22921 agaagggatg gagtaaaaat gaatgggaac gatatggaga aaaaacatga gcataaaaaa
    22981 gaagtagtaa acaaagaaag tacagaatct catgaaaaac agatggagat gaaacatgac
    23041 catagtcaga tggatcattc catgcacatg ggacataacc atggagatat agcaaacagc
    23101 aaacaaatgg accattcgat gcacatggat cacgaacacg gcggcatgga tcattccatg
    23161 cacatgggga atttcaaaca aaaattttgg ctatcgctta tcctagctat cccaatcatt
    23221 cttttttcac caatgatggg aatggaattt ccatttcaag taacgtttcc tggttctgat
    23281 tggctcgtat tgatcttagc aactatacta tttatctatg gcggtcagcc atttttaagt
    23341 ggagctaaga tggaattgaa acaaaaaagt cctgctatga tgacactgat cgcgatgggg
    23401 attacagttg catatattta tagtgtctat tcgtttatcg caaatctgct caatccacat
    23461 acacacgtca tggatttctt ctgggaattg gctactttga tcgttattat gctactaggt
    23521 cactggattg aaatgaatgc tgtgtcgaat gccagcaatg ctttacaaaa actagcagaa
    23581 ttactgccag aatcagtgaa acgcttgacg aaagatggaa aagaagagac agtttcattg
    23641 aaagaagtaa acgaaggtga tcgtttgatc gtccggtctg gtgataaaat gccaacagat
    23701 ggtgtaatct tgaaaggtga aacaattgtt gacgaatcag ctgtgactgg cgaatcaaga
    23761 ggaatcaaaa aacaagccaa tgacaaagtg attggcggtt ctatcaatgg ggatggaacg
    23821 atcgaaatcg aagtaactgg aactggtgaa aatggttatc tagcgaaggt tatggaaatg
    23881 gtgcgaaaag cacaaggaga aaaatccaag ctagagtcat tatctgacaa agtggcaaaa
    23941 tggctgtttt atgtcgcatt aattgtgggt atcctggcat tcatcgcctg gttgttcttg
    24001 acagatcttc ctaatgcatt agaacgtatg gtaacagtct ttatcatcgc ctgtcctcat
    24061 gctctaggtt tagctatccc attagttgtt gctcgctcca catcaatagc agctaaaaac
    24121 ggattacttt tgaaaaaccg caatgcactg gagcaagcca atgatctaga tgtgatcatg
    24181 ctggacaaaa caggaacact aacagaagga aaatttactg taacgggtgt cgaagtactt
    24241 gatgatgcgt tcaataaaaa tgaaatcctt caatatctag gagccttgga agcgaatgcc
    24301 aatcatccat tggctgttgg cattatgaat tatttgaaag aacatgaaat caagccttat
    24361 caagctgaaa atctaaaaaa tctttcagga gtcgggctag aggcaacagt caaaaaacaa
    24421 caagtgaaaa tagttaatga aaaagaagtt gagcgtctcg gattaaacgt agaacaagca
    24481 ttattgaaac cttaccaaga acaaggaaat accatcagct tcctgatttt agaaaatcat
    24541 ctagctgcaa tcgttgcttt aggtgacgta gtaaaaacag aagcaaaaga atttatccga
    24601 acattgaaag aaagaaaaat cactcctgtc atgcttactg gtgacaataa aaacgctgcc
    24661 caagcagtag cagattattt agggatcgaa gaatattatg gcggtttatt accagatgat
    24721 aaagaagctg ttgtccagaa ataccttgac caagggaaaa aagtaatcat ggtgggtgat
    24781 gggatcaatg atgcgccaag tttagcccgt gcttcaatcg ggatggcaat cggtgcaggg
    24841 acagatatcg caatcgattc tgcggatgtc gtattgacaa acagcgatcc aaaagatatt
    24901 ttgcattttc ttgatctagc caagcaaacg cgaaagaaaa tgatccagaa cctctggtgg
    24961 ggggcaggtt acaatattct tgcaattccg ctagcagccg gaattcttgc gccaattggt
    25021 atcatattaa gtccagcagt tggtgctgtc ttgatgtcgt taagtacggt agttgtagct
    25081 ctaaatgctt tgacattgaa aatcaaataa taatgaaaaa aggttgtgac aaaaaacgtg
    25141 tcacaacctt tttttcgagt aatcgatact caatcagctg gttctcggca ttccagaagt
    25201 agattctcca gaaattttat cttatttttt gaagcggctc ccttttgtca gaattttttt
    25261 gtataccgtt tttcttatta tacgcgttat ttctaaatag gtttttttat cgaattttac
    25321 cggatctgct tgtcgtccat atatttctgc ttcatattga gtagttaatc gaatgaaacg
    25381 cagatctagc ttgaattctt gttctattcg caggctatat tcctgcaaag attctgcggg
    25441 tttgcgcggt accaatgttt ccaaagggtt aagaagtttt tgatatgcaa tcgtattagt
    25501 agaagcaaaa aaatgaagat agataagata acttgtccag cgcagagatt ttctgaataa
    25561 caatagaaag aacaacaaga cggataaaca cactccaagg ctgaataaga cgattttata
    25621 agctggtgtg agtgttgttt cttctgatgg aatgacctca gatctctcac tggaaatagt
    25681 ttctgccata ttttcgcttt catttggttg ttcgatagca ggctggacgg tttcggttgt
    25741 ttcagcaact ggttgtgaac ccattggatt ggcaaatgaa ggggttggtt caaatggaag
    25801 ccagccaaat ccaggaaagt atacctctgg ccaagaatga gcatgactat tgagaatgct
    25861 gtattcctca ttcccttgat tatctgtgcc tgttctgagc ccatttgtaa aaccttttgt
    25921 ccagcgagct gggataccaa gtgttcgaag aaggacgacc atagaagttg aaaagttgtc
    25981 acaatagccg acttttgaat caaagaggaa ataatccaca taatcgtgtt ctttcggagt
    26041 atgcccggta tccgttttgg aatagcggaa atttccgctt gtttttaaat actgttagac
    26101 agcttcgact ttacgaatta aagttgcttc atttttggtc aattcgaccg ccaagtcctc
    26161 aatacgttta ggcaaacttt ttggcaattg aagatttgtc tgctgatttt ctaaatcatc
    26221 agcttttaac tgactgcttt ttaattcttc tctcgtaaaa actaaccttt ctgtttgtaa
    26281 agttgcagaa cgtgtcgatt cattcgggac aggaatgaat cgctggcttt caggcaaata
    26341 gtaaagcgtt tgtccactag ctaatgaaaa atcagtcaag gtagtatttc cataaggata
    26401 aggtaagaaa gcttgttttt ttagggtcaa tgtaatgttt tctttgtctt ctataacggt
    26461 ttggtagctc gtgatgtcaa cagggaattc ttggattggt tggatgggtg cgtcctgatc
    26521 ttctatccat ccagaaccgg tgtaatgatt tttattttcg atcttccaat agccatttcc
    26581 tttttggttc gctgtaaaaa caacttcgcc attatcatat aaaggtccac caagctgttc
    26641 atcattctca ctgaagcctg ttcttgcact tccggttaat tgatactcat ggagtgtgtc
    26701 ataaagccct tcatcattaa gatattctcg gatcggttca ctcgtttcct cgagagattg
    26761 gtgtactttc ggaagatata aaggcaagat agcgctaaaa gttaagaaac aaataagcag
    26821 aactgtagtc aatgactgga atcgagtgtt tcccgacaac aaccggatgg agaaaaattg
    26881 ccttgctgcc agatacacga acaaaacagt tactaccgca atcatttcgt acagtaaatc
    26941 ttttttccga aaaacggtaa gcatcatgaa gtaagccatc aaagaaagaa agggtgcgta
    27001 ccaatagtcg taatccacgg tcaatgtaat aaataaatag atcagcaaca ttatgagaaa
    27061 aaaagctgtc ttttctggca aataataaag tttaccttgg gatagatgga agaattcctc
    27121 tgtaactgac gaaaaaaact gtaagatcca taatatggaa aatgtcttcg aaggaaaata
    27181 taaaaacaaa caacctatca ttactggcaa agaaagcagc aaacgtaccc attttttagg
    27241 aatacagcag acaataaggc ataggacacc aatagttagg ataagaaggt agctgttttg
    27301 caattgatta gctactacaa atatcggagc acctacactt agtatcacaa aggagtaaac
    27361 gccacgcagc catttttccc gtttaatcgt caatccattc acctcctggc aatgaacata
    27421 tcttgtcttt ttcttggacc atgagttgtt tttctacgaa ataaacaaga taagccgttc
    27481 gatcttttga caatgtttca atagcttctg agacttcagg agaattctca ggaacaaaga
    27541 ggacgatttg tttttttgca tattttttca agtgtgcgaa aaaagctttc tcatcgccag
    27601 cttccaaaga agcaaaatag gtatgaccgg ggtctttgcc atgaaaggcg cggttgccta
    27661 atatcagaag ttccggctgc ttttcttttc tcaaatgcca taaagaaaaa tacaagtcta
    27721 gcagaggttc aaattttgga ctgggcgaac cccaaaagca aaaaagagga gaacattctt
    27781 cttcttgata atcgtattca cggtataacc actcttgagt atgagcagac aatttccaat
    27841 cgattcggtt gaaagaatca cctggacgat aagcgcgatg ttcccgaaaa tcaagacctc
    27901 tttggtacgc gttcagctgc tgtttttttt ccagaactct ctgtaatttt tctgcttgaa
    27961 tcttacaaaa agcaggcaga acagtcacag gaaaagagat ctctcttttt gcactttttc
    28021 tgaaaagtcc tagactatca taggctgtaa aaatgacagt taatgtttca tggcatcctc
    28081 tttcttttgg ctgccaaagc aattggactt cttgcggttt atttgtaaga atcaaaatct
    28141 cttgtttttt tctgcaaaga ttgctgggaa gccgatttgt aaacgaggga gaggtaaaga
    28201 aaaagttctc ttttttgcta tggaaaaaat aacctgcata ctttttttgc gataaacgag
    28261 tggctgcaat gtacaagtaa ccgaaagttt ctttagaaag ctgcttaatg aaatcaagct
    28321 ccatgataca taaagagtaa aaaaggccaa caagaaccag cctaaagaat tgttgaatac
    28381 aagagcaaaa agcacaatga tgaaatatac cagaaataac acaaaattaa tgaaataatc
    28441 tttgattttt tccattgtct acctcaatct acctcacagg aataggaatt tgtgcaacaa
    28501 gagtatgaat gatttgtttc ttttcgtctt ccttcaatga acgatcgtaa aaaatcaggc
    28561 gatgggaaaa cacgtaagga actaaatcct gcaaatcttt gggcgtgacg taatttctcc
    28621 catggattaa agcataagct tttgctgcct gaataaagga aaggcttcct cgtggactga
    28681 tgccaagctg gatggcagca tgacttcttg ttgctgacac taactgcaag gcataagatg
    28741 ccacttcggg actgacaaag acattcgcca catttgtttt tatttcttct atttcataac
    28801 tgttcaatac ttcatttaag ttatccaaca ctttttcgaa tttatccaaa agtagcaaaa
    28861 gttcgtcatc aaaagaagga taaccgattt gtagtcggaa taaaaaacga tcgagctgtg
    28921 cttccggcaa aggatatgtt ccttcgtact cgattggatt ttgcgtagct aagacaaaaa
    28981 aatgattgtc tagagggtat gtattgttgt cgatcgttgc atgattctcg gccattgctt
    29041 ctaaaagtgc tgactgagta cgaggagtcg tacgattgat ctcatctgcc aacaagatcg
    29101 tggtaaagat cggtcccgga cgaaattcaa actcttttga ttgtgaattg tatacggaaa
    29161 aaccaagaat atcactaggc agcagatcag gagtacattg gactctgctg aaggttccct
    29221 gtacagcttt tgctagtgct ttgaccagta acgttttacc tacaccggga atatcttcga
    29281 ataagacatg accacctgct aatagggcca cgatcatcaa ttggatagtt gaacgtttgc
    29341 cgatgaccgc tttttctaat tcttatatca actgatcgat tttttgtttg gattcttcta
    29401 ttttttcttc cattttcaca tcaccttctc ataaaatcgc tatttatttc attgtaatgg
    29461 aagggaatac atcctacaag caaagtgatc ttattatgtt tttttaaagt acattcgttt
    29521 tgaaatgaga atacgattaa attgagtaaa ataaaaaata atgaggaaga tttgcgtatt
    29581 taaataaata gaaagggagt tttttatctt tttaggagtg aactcatttt gatcaaacaa
    29641 tatgtttaga aatcaggtga gaaatgatcg atgagatggc aaaaactatt atcaaaaaat
    29701 gcacagaatt tattttcact aatcaatcta atgaaagatt tgatctggcg aaaagctgat
    29761 ttagctgaag aactgaaaat cgatgttaaa acagtggatc ggcatctgaa acgtttggcg
    29821 gaaagcgact gtcccattca gctgattctt cataagaaaa ccgttcagtt agtttatgat
    29881 caaggatata gcgaacctta tctcttgttt gtttttctga tccaatcacc cccgttttgt
    29941 ttactgaaaa atctgctatt aaatgaacct tacgagcaga agtacgatcg ttctacgcaa
    30001 gagcgattga gaaagtggtt tggcgagtac cacctttctt tttcttataa aaaagcacag
    30061 atctttggtt cagaaagaaa agtaagatat ctgctgtttt actttttgaa agattttccg
    30121 catttttttg cagatgatgg agagcagtcc ttttctgaga tgttcatcca attattgaag
    30181 caaagaaagg atccggcaga aatcgagatt ttaggagaca gtcgaacact attctttgaa
    30241 ttgtttcctg aattgctatt tagagaggag cagattttgc caataaaaga aaaaaattat
    30301 ctagcaattt tgcgcttgag ctgtaaccag attactgaaa agcggtggaa acggatagaa
    30361 gagacacaag actatcggaa attgacgatt gtgactgaaa agcttgattc gctgttttgg
    30421 ctgaatagcg aatttgagag cgaaagcaaa tcaaagctga taaaagtact ttaccaagaa
    30481 tggcttcgtt acgtaattgg tatccatgac cggctgatag aagaagttcg ttattatcag
    30541 gaaacattag aaaaatttcc caattccaaa gataaactat atcaatttcg gctagcgatc
    30601 caacaagcac ttgcacaccc cgcagtagag tgggggatgc ttttactgaa aacactccag
    30661 gaacgagggt atttagatgt ccaccctcct gaagtgaaaa tcgtcttttt ttttcgctat
    30721 gaacaccaag aagcaagaag acttgctggg gtactgaaca aaacgctaag acatcgaaaa
    30781 cggatctcta tacatgtatg tactcgaaat aagccgcctc ggtatgcaga tctagtgatc
    30841 accgatcatt tttttccact agaccataac gaagatcaaa aaacgtatct gtaccatcct
    30901 tcatttggta tcgaaagact tcttaccgat atcaattact ggttaggcaa aaaccgataa
    30961 aacctttgga aaaaagatca aaacaagtca gtctgtgcta taataaaaag cgattagtaa
    31021 aggaggaaat ccattggaat taaagaaatt agaagttccc acatcttcga tcacagaagt
    31081 gaaacgatcg ccgatggatg tatttgctca agcgcgagac gcgggaactg gcgtatatat
    31141 atttaatcgt gaaaaagtag ctggcgtgat gctgacccaa gaacaataca aaacgctctt
    31201 gcaagaacta gatgtccttc gtcaaacagt gaaagtggag aggaaagaac caaaagaaat
    31261 aaacgaagaa cctattaaca aaacagcttc cgtcgtatcc aatgctgaag ggatggaaga
    31321 gctggcccaa acattacagc actcgctcat tactggtgtg acgattacag caaaaaatct
    31381 agatgaacga atggtagcac taggttttat tacgaaaaag accggatttg gcggagtcgt
    31441 ggatatgctt agcgagttga tggaaactgg gaaaatcaac taccagttaa gaagaaaacc
    31501 gcaaaatcgg acaatcatag ctgaaatcat aggagagcaa aacagtcaat ctcaattgat
    31561 ggataaaatc atcatcaaga aaatttttct acatgagcta taagaaaacc atgttcgcac
    31621 ttctaggcaa gccaggcttt gaagaccttg ccaaagaatt gaacgaacgc ctttaagata
    31681 agggtttttc gctagattta aaaaatcagt gatagaaaca tgttcgctta taaataaaat
    31741 tagtgatagt tttagtgata gtgagcttaa aaaagcactc aatttgagtg ctttt
//
LOCUS       DAXWLH010000012        30374 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXWLH010000012 DAXWLH010000000
VERSION     DAXWLH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.012"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            144..512
                     /locus_tag="ACGE36_000389"
     CDS             144..512
                     /locus_tag="ACGE36_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFF8382815.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            692..1633
                     /locus_tag="ACGE36_000390"
     CDS             692..1633
                     /locus_tag="ACGE36_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFF8382816.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFIALIK"
     gene            1702..2433
                     /locus_tag="ACGE36_000391"
     CDS             1702..2433
                     /locus_tag="ACGE36_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8382817.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2577..4757
                     /locus_tag="ACGE36_000392"
     CDS             2577..4757
                     /locus_tag="ACGE36_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFF8382818.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4777..5259
                     /locus_tag="ACGE36_000393"
     CDS             4777..5259
                     /locus_tag="ACGE36_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFF8382819.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5275..6036
                     /locus_tag="ACGE36_000394"
     CDS             5275..6036
                     /locus_tag="ACGE36_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8382820.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6167..7501
                     /gene="murC"
                     /locus_tag="ACGE36_000395"
     CDS             6167..7501
                     /gene="murC"
                     /locus_tag="ACGE36_000395"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFF8382821.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7808..8293
                     /locus_tag="ACGE36_000396"
     CDS             7808..8293
                     /locus_tag="ACGE36_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFF8382822.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8286..8969
                     /locus_tag="ACGE36_000397"
     CDS             8286..8969
                     /locus_tag="ACGE36_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFF8382823.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9095..11740
                     /gene="polA"
                     /locus_tag="ACGE36_000398"
     CDS             9095..11740
                     /gene="polA"
                     /locus_tag="ACGE36_000398"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFF8382824.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11785..12621
                     /gene="mutM"
                     /locus_tag="ACGE36_000399"
     CDS             11785..12621
                     /gene="mutM"
                     /locus_tag="ACGE36_000399"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFF8382825.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12585..13214
                     /gene="coaE"
                     /locus_tag="ACGE36_000400"
     CDS             12585..13214
                     /gene="coaE"
                     /locus_tag="ACGE36_000400"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFF8382826.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13266..13598
                     /locus_tag="ACGE36_000401"
     CDS             13266..13598
                     /locus_tag="ACGE36_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8382827.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13910..14401
                     /gene="nrdR"
                     /locus_tag="ACGE36_000402"
     CDS             13910..14401
                     /gene="nrdR"
                     /locus_tag="ACGE36_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFF8382828.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14424..15797
                     /locus_tag="ACGE36_000403"
     CDS             14424..15797
                     /locus_tag="ACGE36_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8382829.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15797..16723
                     /gene="dnaI"
                     /locus_tag="ACGE36_000404"
     CDS             15797..16723
                     /gene="dnaI"
                     /locus_tag="ACGE36_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFF8382830.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16852..17292
                     /locus_tag="ACGE36_000405"
     CDS             16852..17292
                     /locus_tag="ACGE36_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFF8382831.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17436..18137
                     /locus_tag="ACGE36_000406"
     CDS             17436..18137
                     /locus_tag="ACGE36_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFF8382832.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18134..19255
                     /locus_tag="ACGE36_000407"
     CDS             18134..19255
                     /locus_tag="ACGE36_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFF8382833.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19270..20502
                     /gene="pepT"
                     /locus_tag="ACGE36_000408"
     CDS             19270..20502
                     /gene="pepT"
                     /locus_tag="ACGE36_000408"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFF8382834.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20634..21542
                     /locus_tag="ACGE36_000409"
     CDS             20634..21542
                     /locus_tag="ACGE36_000409"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8382835.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21577..21849
                     /locus_tag="ACGE36_000410"
     CDS             21577..21849
                     /locus_tag="ACGE36_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFF8382836.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21860..22906
                     /locus_tag="ACGE36_000411"
     CDS             21860..22906
                     /locus_tag="ACGE36_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFF8382837.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23157..25766
                     /gene="clpB"
                     /locus_tag="ACGE36_000412"
     CDS             23157..25766
                     /gene="clpB"
                     /locus_tag="ACGE36_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFF8382838.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25917..26603)
                     /locus_tag="ACGE36_000413"
     CDS             complement(25917..26603)
                     /locus_tag="ACGE36_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382839.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26581..27075)
                     /locus_tag="ACGE36_000414"
     CDS             complement(26581..27075)
                     /locus_tag="ACGE36_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8382840.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27095..28315)
                     /locus_tag="ACGE36_000415"
     CDS             complement(27095..28315)
                     /locus_tag="ACGE36_000415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8382841.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28462..30297
                     /gene="lepA"
                     /locus_tag="ACGE36_000416"
     CDS             28462..30297
                     /gene="lepA"
                     /locus_tag="ACGE36_000416"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFF8382842.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10186 a         5405 c         6551 g         8232 t
ORIGIN      
        1 aaaaacaacc aacttattta ttattaaaga aaagcgtatt taagtaggac gattctgttg
       61 agagctttcg tagattttcc cattttccta tggtaaactg acaaagttga gaatgttaga
      121 acagatcgtt taggaggaaa cagatgttta cgaatgaaat aaaaatcatg ctttatgtgg
      181 acgatgtaga aaaaaatgca acgttttgga gagcagttgg ctttgcggaa aaagatcgtc
      241 aagaaatgga tgggacgctt ataattgaag taggagttga ggacagtcaa acctcatttc
      301 tgttatatga tcgagcattt gttgagtccc attcacctgg cacagctact agtgccccat
      361 ctttgatgtt ttacagtaaa gatatctttg gactatataa aaaaatgcag acgcaatcag
      421 tcacattagg tgatatggta cagcttggag aagaatatgt ctttaacttt gctgatcccg
      481 atggcaacta ctttgcggtt acaggtaaat aatagaaata aagactttgt ttttctgtgc
      541 gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac aaaaaacttt
      601 ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc tttatactgt
      661 ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc tttattacaa
      721 ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct tttgcaaatc
      781 tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta ctgtgtctgg
      841 aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc gatttgctga
      901 tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt ttgtaagata
      961 ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc aagtgacagg
     1021 tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga cagtggtgat
     1081 tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg tacgcccaga
     1141 tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag aatttatgga
     1201 aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt ctggttggct
     1261 tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta cttatggtaa
     1321 ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg atgaacggat
     1381 cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt ttgacggaac
     1441 accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc
     1501 cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga aacatccgcg
     1561 caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc ggtttatagc
     1621 tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg cgctatacta
     1681 gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga tgtccatggc
     1741 aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca tcagcttgtt
     1801 ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt attaggaaag
     1861 caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga atattttttg
     1921 caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg tggaaaagaa
     1981 acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac tgaaatctcg
     2041 atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg ccccttgtat
     2101 tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa gaaagactgg
     2161 cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga agggaaaaac
     2221 aatacaggta agacaattgt atttggacat acgattacac caatgctcca tggagatatg
     2281 cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg ggctgtattc
     2341 ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga tattgaatat
     2401 cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa ggagctgtga
     2461 ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta gtttaaccca
     2521 ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc attttaatga
     2581 cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca gactatcgtc
     2641 ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt ccttttatcg
     2701 cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt gaaatcgaag
     2761 agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg
     2821 atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc gtgaaaatgc
     2881 aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa gcaacgattg
     2941 ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca gatggagagg
     3001 tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct gccgaagaag
     3061 cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca aaatttcgga
     3121 cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc aaggatgagc
     3181 aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc gtccataaaa
     3241 tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt ctgaaagtat
     3301 tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata gtcaaaaatc
     3361 ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat aaacgcttta
     3421 ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca gatgagcaag
     3481 caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca ctaaaaggta
     3541 aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca gtcgtagatg
     3601 cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca gcgcaagcaa
     3661 aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa gtagatatgg
     3721 tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct gagcagttga
     3781 aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct tctgtttact
     3841 ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa agaagtgctg
     3901 tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc gatcctaaag
     3961 ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct gaacaattgg
     4021 attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca gcaagcccgc
     4081 agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt gtcatttatc
     4141 gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac
     4201 caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa aacattttgg
     4261 acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta acttcagtcc
     4321 aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca cgattatccg
     4381 tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat attttagctg
     4441 ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta ttacgtacag
     4501 atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg gtccgtaatg
     4561 tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt gtccatatat
     4621 cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg
     4681 taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg acgatgcttt
     4741 ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga actgcagcag
     4801 ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca agcaatgttc
     4861 aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca tttagatttt
     4921 aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat caaacatgaa
     4981 ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga tatggatttc
     5041 aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac gaagaaacag
     5101 ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg caagagaaga
     5161 atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg gattgagaca
     5221 aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag gcctttggaa
     5281 aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt agccgcacct
     5341 gaagagggaa attttgagga actgacggca cctacactta ttttagctgg aaactgtctt
     5401 cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga tcaattgttt
     5461 ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga aaagcaagga
     5521 tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc
     5581 tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc tggagagaac
     5641 gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa aatcttattg
     5701 atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt atggcaaaat
     5761 gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca cttttctgaa
     5821 gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata tttccatgcg
     5881 ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg tccgaaagga
     5941 aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta caccagaatt
     6001 aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc gaatcctcct
     6061 ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga gcgtgttgaa
     6121 tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg aaaatcaaaa
     6181 taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt
     6241 acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct ttacacaaag
     6301 agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg taaaaccagg
     6361 gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc agcgagcaaa
     6421 agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta tccaaaatta
     6481 tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac tgctctctca
     6541 tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag gacatggtga
     6601 tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt tccttgccta
     6661 ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt attatacaag
     6721 tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa aagctatttt
     6781 tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga tttattatta
     6841 tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa cttctggttc
     6901 tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac cagcattcgg
     6961 taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg aaaaactgga
     7021 tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc gattcagtga
     7081 aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg cagagatcaa
     7141 agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg cagttttcca
     7201 accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag cacttgattt
     7261 agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac aaggaaacgt
     7321 taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa
     7381 tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg ctggagatgt
     7441 tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa atgtactgta
     7501 atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc
     7561 aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc tgcggtaata
     7621 agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt attatttgat
     7681 gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc aagggagcag
     7741 aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa actggagggt
     7801 ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg aaatcgagga
     7861 aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc tgtatttggg
     7921 attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa aaacagaata
     7981 tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg caatttcaaa
     8041 gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta ttgaaccggt
     8101 tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata agatgaagga
     8161 tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt
     8221 cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa gtgaaggtga
     8281 attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat gcaaaagttt
     8341 atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg gctctttggg
     8401 tatttccttt gcaattagca acttttatca ataattttcc actaggcttt gtgggtctgt
     8461 ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag aatccgagct
     8521 tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta gcggttactc
     8581 ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact ttgatgttca
     8641 tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt ggaagagcag
     8701 gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc attttgagaa
     8761 gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca gggattacag
     8821 cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca gcacatagtg
     8881 gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg ttcttggcgt
     8941 ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt tggggctata
     9001 ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga
     9061 atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat tattactcgt
     9121 tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt
     9181 caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga tgtttgaaaa
     9241 tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac
     9301 ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac ctggcgaatt
     9361 caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac agtattacga
     9421 attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag tagacaaaga
     9481 tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag ctagtaaaga
     9541 agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata caccagaaca
     9601 tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga agggactagc
     9661 cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa
     9721 gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg aaatgaagca
     9781 aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat cgaaaaaatt
     9841 ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga agtatgaagg
     9901 gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc agttcctttc
     9961 taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg aagtcgttca
    10021 tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa tgatgggcga
    10081 caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga aaatttatgt
    10141 cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca ctgatccgac
    10201 tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc gctatactgg
    10261 aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg atacgaatga
    10321 aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata tccaatcaga
    10381 tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg aactcttttt
    10441 tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat tgagccaaga
    10501 actggcagaa aaaaaccaag aagatctttt ccggaaaatg gaactgccat tgtctatcat
    10561 tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac aagaaatgaa
    10621 gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag aagccggcga
    10681 agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa aaatgggatt
    10741 acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac tggaacagct
    10801 tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg caaaaattca
    10861 atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag tgcatacccg
    10921 ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta atttgcagaa
    10981 catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc ctagagaaaa
    11041 agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac ttgcgcatat
    11101 ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc atgccagcac
    11161 agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca
    11221 ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac tttcacaaaa
    11281 tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg aaaaataccc
    11341 aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag gctacgtcga
    11401 aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt acaatatccg
    11461 ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg ctgatatttt
    11521 gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc aagctacgat
    11581 gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt
    11641 agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac cactgatcac
    11701 tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta agacagagca
    11761 atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa
    11821 ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag
    11881 atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga aaccatccaa
    11941 tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga actgatctca
    12001 cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat cgataaacat
    12061 acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga tgtacgaaaa
    12121 tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa
    12181 ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa tggactgaaa
    12241 aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac tggtctaggt
    12301 aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca accggcgaac
    12361 acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt tttggatcgt
    12421 gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg agaatcaggc
    12481 ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat acgttgtgga
    12541 acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc gaattgtcaa
    12601 aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata gctaccggta
    12661 aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat ggcgatgtga
    12721 ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta tcagtgtttg
    12781 gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg atgattgttt
    12841 ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt atccgaaaag
    12901 cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt gtagatatcc
    12961 cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc gtttatacta
    13021 cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta gaacaagcgc
    13081 agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt
    13141 tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg ctttttgcta
    13201 atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga aagtgaggga
    13261 tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa
    13321 tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag
    13381 cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac cagcaggcag
    13441 aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca cctgatcgta
    13501 gtaaaacacc gctcccagaa agtgaccaca ttcccgatga gcaaaaaatc gaggaacagc
    13561 gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg
    13621 acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag atgactaaat
    13681 cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa aattgaaatt
    13741 tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct tatatccgtg
    13801 ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca actagttgta
    13861 aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa tgagatgtcc
    13921 cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg atggacgtgc
    13981 gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat ttgaacgaat
    14041 tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt tcaatcgcga
    14101 taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta tggaacaaat
    14161 ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa acgaagtatc
    14221 gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg aaatcgctta
    14281 tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct taaaagagtt
    14341 gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag aaaacaatta
    14401 agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa ccaaaaaata
    14461 tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc ttgctgtatc
    14521 tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg ttatcagaaa
    14581 tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt agtagtctag
    14641 gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc ggcttactgg
    14701 atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag ctgttagaac
    14761 cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt ctggaaaaag
    14821 ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg catacagcag
    14881 agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat gaagcagtgt
    14941 acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa tctgaatcag
    15001 tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat tggctggcaa
    15061 aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg tatcatcagc
    15121 tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat gactttagtg
    15181 aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag agacgtccgg
    15241 tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc tcgtcaaacg
    15301 aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt ccaccgatga
    15361 aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa gaaaactggt
    15421 tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat gttttgctga
    15481 attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta aatacagtgg
    15541 caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag cacattcggc
    15601 aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat caaactggca
    15661 agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa gatgaaaaac
    15721 agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac ctggcaaaaa
    15781 aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca tcagcaaacg
    15841 caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg cggatgttca
    15901 agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa aaagttacgc
    15961 taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc caacgatgat
    16021 cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg tcacttatgt
    16081 accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc gagtaaaagc
    16141 aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac gtacagatgg
    16201 acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg ctgatccaaa
    16261 agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa catatttatt
    16321 aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag tccattttcc
    16381 gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag aaaagttaga
    16441 tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg cgatgagtag
    16501 ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg aacagttacc
    16561 aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt ccgtcactca
    16621 acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac gttatttgac
    16681 aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg acaagtccaa
    16741 ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac agaaatgacc
    16801 ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt tatgagggct
    16861 attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg cggtagcgca
    16921 gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa aacaagcagt
    16981 acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtatcac tcaagtagaa
    17041 gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga agatccagcg
    17101 aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc tgaaaaaata
    17161 agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt agttgagatg
    17221 ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat tgatgtttca
    17281 ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt atagaacgaa
    17341 aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct tattttattt
    17401 ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat ttatctcagc
    17461 gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac attggatcag
    17521 atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat gcagttgctg
    17581 gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa gatggattag
    17641 aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca caagatggta
    17701 ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc ctagaagctg
    17761 gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca aacatcggag
    17821 agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca gaaaaaatac
    17881 ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa aaaccagtta
    17941 cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa agtgagatct
    18001 tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa cagctcaaac
    18061 aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa atgattgagg
    18121 aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg aaagctattg
    18181 tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa cattggataa
    18241 gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg aagaagcaat
    18301 caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc ctgtaaaacg
    18361 gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac atgatattgc
    18421 cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg actggttttg
    18481 tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga ttcattataa
    18541 aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag tattagctgc
    18601 agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga ttggacacgg
    18661 tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga agacagaaca
    18721 agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac aagtgattca
    18781 agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg ctatagatga
    18841 accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa tttctataga
    18901 ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa tagtccagcc
    18961 aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg gtgaaaaatt
    19021 ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata tctattatca
    19081 tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc attatattga
    19141 atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag aagaaaattg
    19201 ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta attaaaagga
    19261 gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa aacagaaaca
    19321 cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc ttttgcgcaa
    19381 gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa tgaagataac
    19441 ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc cattggattt
    19501 atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca aatcattgaa
    19561 aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac attgaataca
    19621 aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac agatggcagt
    19681 acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc aatggagatc
    19741 ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg tcctgatgaa
    19801 gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga ttttgcttat
    19861 acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc tgctcaagca
    19921 aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac aatgatcaat
    19981 gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt tccagaaaaa
    20041 acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc tgaagaagct
    20101 tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg caaagcacaa
    20161 atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat caaagtagat
    20221 atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag catcgtggaa
    20281 ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc agttcgtgga
    20341 ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct atttgccgga
    20401 ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga aaaagcaaca
    20461 gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa aagaacagct
    20521 tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa agggcaggac
    20581 aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa agaatggaaa
    20641 agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa ttagcacgct
    20701 ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta gctgaaactg
    20761 gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa gctgatactt
    20821 tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc caaaaaacat
    20881 tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt gcacgtgttc
    20941 ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc aatgtacgga
    21001 aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca atcggaatct
    21061 tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc tcgtggatct
    21121 ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg gatttagaaa
    21181 cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg tctaactccg
    21241 tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa tttcatgtaa
    21301 cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga atcatgagag
    21361 gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg aaaatccaag
    21421 cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat cttccagttg
    21481 aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa gaaaaagctt
    21541 agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt acacaatcct
    21601 ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg gtatgataaa
    21661 catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt tgtcagcctg
    21721 ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa aactcggaaa
    21781 aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct catttatttt
    21841 ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca ttctgaatct
    21901 gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat catttgcaga
    21961 tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg ttatattagg
    22021 gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac ctttcgcgcc
    22081 gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt accgaatcgt
    22141 ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa aaggaatgaa
    22201 gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac tggcagtgat
    22261 cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta atggtccatc
    22321 aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg cctttttatt
    22381 ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga ataagttatt
    22441 taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct actctttaag
    22501 ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt atctatggaa
    22561 tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg aaaaccatcc
    22621 taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc tttcaggggt
    22681 tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat tattcagacc
    22741 agttgttgga acagttgacg cagtttcttc gcttttactg tatcgtttca gttcctttat
    22801 cttgccattt ctctatggat ttgtgtatgt gattgctgaa cggcgaagag tgatcaaaga
    22861 agaaataata gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct tttataagat
    22921 ctaccctttt tatcaaatca aagaaaaacg ttatggataa gataatttta cgtttgtttg
    22981 gctctttttt tatatacata caatcaattt tgttttttta agatttatat aagtcgagaa
    23041 aaaaagtcaa aaaactatgt gtatattttg accaaaattg accaaagcga tataatagtt
    23101 tttgtaaaga tcaaagaaat aagtgatctt acctttaatt aggagggtat acctgtatga
    23161 atattgagaa aatgactacg acgcttcaag aagcgattgc tgaagctcaa cagattgcgg
    23221 tcactcgaaa acatcaagat atcgacattg cccatgtatg gaaaatcttt ttacaatcta
    23281 atcattttgg tcgaaatttc tatacggatg ccggattgga tgtcgaatct tttgaacatg
    23341 aaattgatcg tctactggat gagtatccag ttgtttctgg tggaaacgtc caatatggac
    23401 aaaacctcag ccagaatttc tttcgattat tgaatgaagc agaccaaata cgtgaaagct
    23461 tcggcgacga gtttctttca acggaagtcg ttattttagc gttgatgaaa ttaaaaaatt
    23521 atccgttaac tgtttatttg aataaaaacg gattatctga aaaagaattg cgtaaaaaca
    23581 tcgaagagat gagaggagga gaaagagtga cctcacaaaa ccaagaagaa caatacaaag
    23641 cattagaaaa atacggcgtt gatttagtac agcaagtcaa aagcggaaaa atggatccaa
    23701 tcatcggtcg tgatgaagaa atccgtgatg tcatccgtat tctttcacga aaaacaaaaa
    23761 ataatcctgt tctgatagga gaacccggtg tagggaaaac ggcaatcgtt gaagggcttg
    23821 ctcaacgtat cgtacgcaaa gatgtgcctg aaaatttaaa agataaaacg attttttcat
    23881 tagatatggg tgcattgatc gctggagcaa aattccgtgg tgaatttgaa gaaagactaa
    23941 aagcagtctt gaaagaagtc aaaaaaagtg atggacgtat cctcttgttc atcgatgaaa
    24001 tccacaatat cgtaggggca ggaaaaacag aaggaagtat ggatgccgga aatctgttga
    24061 aaccaatgct tgctcgcggt gaattacatt tgatcggtgc cacaacttta gatgagtacc
    24121 gtcaatatat ggaaaaagac aaagcgttgg aacgtcgctt tcaaaaggta ttagtcaaag
    24181 aaccaacagt ggaagatacg atcagtattc tgcgtggatt aaaagaacgt tttgagatcc
    24241 accatggtgt taacatccat gacaacgcat tagtagcagc agctacttta tctgaccgtt
    24301 atattactga tcggttctta ccggacaaag caatcgattt gatcgatgaa gccagtgcaa
    24361 cgattcgggt agaaatgaac tctatgccaa cggaattaga tcaagttact agacgtttga
    24421 tgcagttgga aatcgaagaa gctgcactga aaaaagaatc agatgatgca agtaaaaaac
    24481 gcttgaaaaa tttgcaagaa gaattagctg aacttcgtga agaagcaaat gcgatgaaga
    24541 tgcagtggga aacagaaaaa gaagaagtca attctgtttc agcaaaacgt gctgaaatcg
    24601 ataaagcaaa acacgaatta gaagatgcag aaaataacta tgatttagaa cgcgctgctg
    24661 tattgcgtca tggtacgata cctcaattag aaaaagaatt gaaggaatta gaagctaaag
    24721 caaaagacag cgagattaag atggttcaag agtccgttac cgaaaatgag attgctcaag
    24781 tcgttggcag attaacgggt atccccgtaa cgaaacttgt agaaggtgaa cgagaaaaac
    24841 taattaaatt gaatgaaaca cttcataaac gagtgattgg tcaagatgaa gcagttgatg
    24901 cggtcagtga cgcggtaatt cgttcacggg ctggattgca agacccaaat cgtcctctag
    24961 gttctttcct tttcctagga ccaactggtg tcggaaaaac cgaattagcg aaagcgttag
    25021 ctgagaactt gtttgactca gaagatcata tggtacggat cgatatgagt gaatatatgg
    25081 aaaaacatgc tgtgtctcgt ctagtcggag ctcctccagg ctatgtaggt tacgaagaag
    25141 gtggtcaatt aacggaagct gtcagacgaa atccttatac aatcgtcttg ttggatgaaa
    25201 tcgaaaaagc gcatcccgat gtcttcaata tcttgttaca agtcctagat gacggacgct
    25261 tgactgattc aaaagggcga gtagtagatt tcaaaaatac ggttctgatc atgaccagca
    25321 atatcggttc acagttactg ctggaaggcg tgactgctga tggaacgatt ccagaagcag
    25381 tggctgaaca agtcaacacg ctattacgtg gaaacttcaa accagaattc ttgaaccgaa
    25441 tcgacgacac gattttattt acaccattga gcctagacaa cgtgaaagga atcgttgaca
    25501 aaatggtggc gcaattggca caacgtctgg aacatcaaga aatcctgctg acaatcagtg
    25561 atgaagccaa aacgtggatt gctgaaaatg cttatgaacc agcatatggt gcaagaccat
    25621 tgaaacgatt tatcactaag gaagtagaaa caccactagc taaagaaatc gttgcaggac
    25681 atgttatgcc taaatcaaaa gtaacgatta ctttacttga tggacaactt catttcaaaa
    25741 cagaagagct agaagaaatc gtctaatctt ccattgagac tataaaagaa aaaaggggtc
    25801 tgacacaact agtgtcagac ctcctttttt caaataaaca gtgttccaga agtagtttct
    25861 tcgaaaaata agccgaaatt tcttgaagct gcctgcttct gtcacaacct cttttattag
    25921 gtatttttca ctttttctaa tttataactt tcaaagtaac gctcatcttt tttactttga
    25981 gaccatgcaa acaatcccat taaggcagca cctatcgtat ttgtcagcaa gtcgcccatc
    26041 gtatccatca atgctgctcg tccgacaaac agtgtgccat cagaagcagc aaaacgttga
    26101 agattcatgc ctaagaactg gtcgcataag aattcccaga actcccaaaa cacgccgcaa
    26161 agtccagcaa acgcaaagcc gaataagagg aacaaccaag gactggtttt tgatatctca
    26221 gcatctttca tcaagtaacc aatcaatcca tagccaagag ctgtcaggac cattggactc
    26281 accgcatgta agattttgtc ccaaaaagag atgatactga tcaaatgcat ccctgtgcct
    26341 aaaaagacag agatgaacaa gaaaaaccag taaaacaaaa cgattgctgc tggtatttta
    26401 agtttgaaaa ctttcaaaat gacttgtggc atgaaaatca atgcaatccc taataaagat
    26461 tccacgataa atgtgattcc ttgagaagtc gaatattttt gtgtgataaa ttcataaaaa
    26521 ttatagcaga caacgaatac accaaaagca agtaaagcta tttgcacctt tttgatttct
    26581 ttaatcactg aaagtccctc catctaaaaa tctttttact tctgaaagca actcaatttc
    26641 tgaagaaacc acttttccgt tcaatttgat caaacctatt gtatataagt tcaggtagtg
    26701 aaattgattt tcagctgttt cttgtagtgc agcgattttt ttcgggtggg ttccgccttg
    26761 ctgtcgagta tcagtatata agccgatcac tgggatgcct ttcgcataag ccacaccgat
    26821 ttccgatgct accccggcat cgatcgtcag cccatctagt aaagcaatca ttaaatcact
    26881 agccaacact ttttctgtgt cagcaagagc aatcattcga ctgtctgcat aagcagtttt
    26941 gtcattgatt gcttcatttt cttgaggaag atagatcgaa agctcagaag agagctctct
    27001 gatttcttta actaactgtt ggttatataa caaatcactt ttagcaaaca ttggtgcggc
    27061 aaaataaatt ttcatctaaa catcatcctt tttatcaaaa ataatcgcta aagtaagctt
    27121 ccatgtttgg aatgatacgc tctagtgact tcagtgtttc ttcatctgtt tctaaccgtt
    27181 cgattcttgc caaataagaa gcacggcgat aattcatcat caagcaagtc aatgtttgga
    27241 tatccagtcg aacggatttt cctataggtt catttaagac acggacttgt ccctgttcat
    27301 cccatgttaa agcgaagaca ccattattcc aaggagcgac aggatcttca atgatgaaat
    27361 gaaaagcatc agctgttcca acaaacggaa accgttgaag gaattctttt acatcaacga
    27421 tccgagccat aaaatatggt tcgatctgct ctttgatctg actatcttca atcaaaaatg
    27481 ctaaaggctc atttttataa atatctccat gtacccagta gatcatggaa aaatgtgcgg
    27541 aaataaagtt ccataagcca tttcgagctt cttgatcaag atagaacatt tcttttacgt
    27601 gaaatatttc ttcagctacc cagtagaaca gaacacctct tggttcatgg ttgctatcgt
    27661 agtagacagc ggctgtgcgc tcatcttcat tttcgaaacg ccaatactct tcccaatgaa
    27721 aatcactgcg gaataaagca ccatggtttt gttgggcaaa tttgtcataa acggaaaata
    27781 cgtctggatg atcaactggt aatcgctcaa ccataccact tactgggatt tgtttaggta
    27841 gctgtgtatc tctgatttta aaggaaagtt tatcagacat gatttcccat cctttacgcc
    27901 gatagtaggg gatactgtaa ggatacaaat aagagatcca ttgcttattc ttgcgcatat
    27961 tatccaatgc aacgataatt agatcttgca tcaatccatg gttagcatat tctggataag
    28021 ttccaactcc agtcacacca cccattttat aacgtgcacc gtgaatattg acttcacaag
    28081 gatagatagc gatttgagag atcagtttat tttcgtggaa ccatccgaac actttcgata
    28141 cttccaaaat cggttttttg gaacggataa aagctcgttt attctcaaac ccgctttcct
    28201 cgatgtctgc ttcagtcact tggaacacat aggaaagcaa ttcattgaat tgatcgatat
    28261 gttcttgagt gaccggtttt aattcaagat tttctctaaa atctttttga tccatttttt
    28321 catctccatt tattagcttg ataacagtat agcaaaattc tacaaaaaaa acttctatag
    28381 ctgactaatt aaaaactctt taaattactg cttttcgttg gtataatgga aagtgctgat
    28441 tatatgaaaa gaggagacgc tatggatata aagaaaatga aagaacgcca agagcagatt
    28501 cgtaattttt cgatcatcgc tcatatcgac catgggaaat ctacattggc cgatcgtatt
    28561 ttagaaatga cgcacactgt gacgtctcgc gaaatgcaag atcagctgtt agactcgatg
    28621 gatttggaac gtgaacgtgg aatcacgatt aaattaaatg ctgtagaact caattacaca
    28681 gcgaaaaatg gagaaacata tatctttcac ttgattgaca ccccagggca cgtcgacttc
    28741 acctatgaag tctctcgaag ccttgctgcc tgtgagggtg ccgttttagt ggtcgatgca
    28801 gcacaaggaa tcgaagcaca aacattggcc aatgtttatt tagcgttaga caatgatttg
    28861 gagatcctgc cagtcatcaa taaaatcgac ctacccgcag cagatcccga acgtgtccgt
    28921 caagaaatcg aagacgtaat cggaatcgat gctagtgaag ctgtattagc cagtgccaaa
    28981 tcagggattg ggattgaaga tattctcgag cagatcgtgg aatatgttcc tgcaccaagt
    29041 ggagacttag atgcaccgct aaaagctttg atctttgact ctatttatga tagttaccgc
    29101 ggtgttgttc taaatgtccg gatcacggac ggtatggtca aacccggaga caaaatcaaa
    29161 ctgatgagta atggcaaaac atttgatgtt acagaggtcg gggtcttctc gccaaaagca
    29221 gttgctagag actttttgat ggtcggtgat gtcggttata tcacagcaag tatcaaaact
    29281 gtccaagata cacgagtagg ggatacagta acgttagcag acaatcctgc cgctgaagct
    29341 ttaccaggtt accgaaaaat gaatccgatg gtttattgtg gtctttatcc aattgatact
    29401 tctcgttaca acgatttgcg ggaagcttta gaaaaactgc aattgaatga tgctgcttta
    29461 caattcgaac cagaaacctc tcaagcatta ggttttgggt tccgctgtgg attcctcgga
    29521 cttcttcata tggatgtcgt ccaagaacgc ttggaacggg aattcaatct agaattgatc
    29581 acgactgcac catccgttat ttaccatgtt aataaaacgg atggtacgac tgctacagta
    29641 gataacccgg cagattttcc tgaacctgta acgattcagg atgtagaaga accattcgtc
    29701 aaagcacaga tcatggtccc aaatgatttt gtcggtgcag taatggaact ttctcaacga
    29761 aaacggggag aattcattac gatggattat ttagatgatt accgagtaaa tgtcgtctat
    29821 aatattccgc tttctgaaat tgtctttgac tttttcgaca aattgaaatc aagcacgaaa
    29881 ggctatgcct cattggatta tgaaatgtct ggttatcaaa aaagcaagtt agtgaagatg
    29941 gacatcttgc tgaatggaga aaaagtcgat gcattaagct ttatcgttca ccgagacttt
    30001 gcctatgaac gtggaaaagc aatcgttgaa aaactgaaaa aactaatccc acgccaacaa
    30061 tttgaggtgc caatccaagc agctattgga caaaaaatcg ttgcccgttc agacatcaaa
    30121 gccttgcgta aaaacgtctt ggctaaatgt tatggtggag atgtttctcg taaacgtaaa
    30181 ctcttagaga aacaaaaaga agggaagaag cggatgaaac aaatcggctc cgtcgaagtt
    30241 ccgcaagaag ccttcatggc cgttctgaaa atggacgagg acgaaccgaa gaaatagcaa
    30301 gggtttgttt gaaaacagtg taaaaaataa accatgttta catggatttg ccaccggttt
    30361 gccaccggtg gttt
//
LOCUS       DAXWLH010000013        29554 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXWLH010000013 DAXWLH010000000
VERSION     DAXWLH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.013"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            134..1003
                     /locus_tag="ACGE36_000417"
     CDS             134..1003
                     /locus_tag="ACGE36_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8382843.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1212..2237
                     /locus_tag="ACGE36_000418"
     CDS             1212..2237
                     /locus_tag="ACGE36_000418"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8382844.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2276..3226
                     /gene="manA"
                     /locus_tag="ACGE36_000419"
     CDS             2276..3226
                     /gene="manA"
                     /locus_tag="ACGE36_000419"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFF8382845.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3320..5023
                     /locus_tag="ACGE36_000420"
     CDS             3320..5023
                     /locus_tag="ACGE36_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8382846.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5025..7058
                     /locus_tag="ACGE36_000421"
     CDS             5025..7058
                     /locus_tag="ACGE36_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFF8382847.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7069..7509
                     /locus_tag="ACGE36_000422"
     CDS             7069..7509
                     /locus_tag="ACGE36_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8382848.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7709..8836)
                     /locus_tag="ACGE36_000423"
     CDS             complement(7709..8836)
                     /locus_tag="ACGE36_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8382849.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8839..9516)
                     /locus_tag="ACGE36_000424"
     CDS             complement(8839..9516)
                     /locus_tag="ACGE36_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8382850.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9780..11546
                     /locus_tag="ACGE36_000425"
     CDS             9780..11546
                     /locus_tag="ACGE36_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFF8382851.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11561..11998
                     /locus_tag="ACGE36_000426"
     CDS             11561..11998
                     /locus_tag="ACGE36_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8382852.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12011..13162
                     /locus_tag="ACGE36_000427"
     CDS             12011..13162
                     /locus_tag="ACGE36_000427"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFF8382853.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13224..13922)
                     /locus_tag="ACGE36_000428"
     CDS             complement(13224..13922)
                     /locus_tag="ACGE36_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFF8382854.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13915..15267)
                     /locus_tag="ACGE36_000429"
     CDS             complement(13915..15267)
                     /locus_tag="ACGE36_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFF8382855.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15490..16890)
                     /locus_tag="ACGE36_000430"
     CDS             complement(15490..16890)
                     /locus_tag="ACGE36_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8382856.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17051..17788
                     /locus_tag="ACGE36_000431"
     CDS             17051..17788
                     /locus_tag="ACGE36_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFF8382857.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17913..18392
                     /locus_tag="ACGE36_000432"
     CDS             17913..18392
                     /locus_tag="ACGE36_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFF8382858.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18389..19156
                     /locus_tag="ACGE36_000433"
     CDS             18389..19156
                     /locus_tag="ACGE36_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFF8382859.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19386..19955
                     /gene="lepB"
                     /locus_tag="ACGE36_000434"
     CDS             19386..19955
                     /gene="lepB"
                     /locus_tag="ACGE36_000434"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8382860.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20059..20661)
                     /locus_tag="ACGE36_000435"
     CDS             complement(20059..20661)
                     /locus_tag="ACGE36_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8382861.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20767..20997
                     /locus_tag="ACGE36_000436"
     CDS             20767..20997
                     /locus_tag="ACGE36_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382862.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            20988..21236
                     /locus_tag="ACGE36_000437"
     CDS             20988..21236
                     /locus_tag="ACGE36_000437"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8382863.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21342..21926
                     /locus_tag="ACGE36_000438"
     CDS             21342..21926
                     /locus_tag="ACGE36_000438"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFF8382864.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21929..23002
                     /gene="prfA"
                     /locus_tag="ACGE36_000439"
     CDS             21929..23002
                     /gene="prfA"
                     /locus_tag="ACGE36_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFF8382865.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            22995..23834
                     /gene="prmC"
                     /locus_tag="ACGE36_000440"
     CDS             22995..23834
                     /gene="prmC"
                     /locus_tag="ACGE36_000440"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFF8382866.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23827..24006
                     /locus_tag="ACGE36_000441"
     CDS             23827..24006
                     /locus_tag="ACGE36_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382867.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24028..25053
                     /locus_tag="ACGE36_000442"
     CDS             24028..25053
                     /locus_tag="ACGE36_000442"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFF8382868.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25228..26472
                     /gene="glyA"
                     /locus_tag="ACGE36_000443"
     CDS             25228..26472
                     /gene="glyA"
                     /locus_tag="ACGE36_000443"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFF8382869.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26625..27572
                     /locus_tag="ACGE36_000444"
     CDS             26625..27572
                     /locus_tag="ACGE36_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFF8382870.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27706..28335
                     /gene="upp"
                     /locus_tag="ACGE36_000445"
     CDS             27706..28335
                     /gene="upp"
                     /locus_tag="ACGE36_000445"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFF8382871.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28474..28716)
                     /locus_tag="ACGE36_000446"
     CDS             complement(28474..28716)
                     /locus_tag="ACGE36_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382872.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28837..28909
                     /locus_tag="ACGE36_000447"
     tRNA            28837..28909
                     /locus_tag="ACGE36_000447"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28870..28872,aa:Thr,seq:cgt)
     gene            complement(29028..>29554)
                     /locus_tag="ACGE36_000448"
     CDS             complement(29028..>29554)
                     /locus_tag="ACGE36_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFF8382873.1"
                     /translation="SLTITGFIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVIS
                     AAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPV
                     ASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHV
                     IFDFTAILLVVLFS"
BASE COUNT        10055 a         5216 c         6029 g         8254 t
ORIGIN      
        1 ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt tttttaacaa
       61 aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct tgacgtagag
      121 gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa aaagtgtagc
      181 cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg ttggctttga
      241 tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg gaagtgaaat
      301 gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca aaaagctgaa
      361 ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt gtcgtatccg
      421 taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg ggattttgct
      481 cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga acgaccaaag
      541 agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag aaaaattcag
      601 taaactatca acaggttctt attttgaaaa aatccattta aatgttacta acttaacaaa
      661 agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag aagagggcat
      721 ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg gaggcgcaat
      781 tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga tcgatactga
      841 aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct tcttagacaa
      901 aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt tcgtcagtga
      961 gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc tttcttaacg
     1021 attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct agagatagaa
     1081 agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt ttgtgtaaaa
     1141 aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg atagaaaagc
     1201 aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg gaagaaggat
     1261 tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca tttacagccg
     1321 gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg tttgatgcag
     1381 cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta gaagatggaa
     1441 agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat cagacaatca
     1501 ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca aacagagata
     1561 agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt gagcaactag
     1621 gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg ctagaggaaa
     1681 tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca ttaggaatga
     1741 tgaacagctt attccttcct tttccttcca aaaaaatcgt tcccacagaa caactgcctt
     1801 cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg gctgaacatg
     1861 caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc caaatgtatt
     1921 tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt gattctttac
     1981 tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac ttaaaaaagg
     2041 atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg tttttcttga
     2101 ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat cctgtcgatc
     2161 atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg aaagagaaag
     2221 cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt gaattatgca
     2281 ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa gtcgtttacg
     2341 ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg cgatcagtgc
     2401 gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa gattagatga
     2461 actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt ttccattatt
     2521 gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag atgatgccta
     2581 tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca tcgatgctga
     2641 acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac tagctgagat
     2701 gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa aaggtgattt
     2761 cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga ttttagaaac
     2821 acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag atgcgcaagg
     2881 acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc ctgcgaaaac
     2941 accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca cttatttaga
     3001 aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt tcaaaaaaca
     3061 agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg ttgacggaaa
     3121 aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt ctgagtgggc
     3181 ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc aaatagttcc cagatagctg
     3241 aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc catcaataaa
     3301 tagaagtctg gtgaaaaaga tggaatacaa atggaaagca aacaacagca gtcgcgtgtc
     3361 tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc caaatctaag
     3421 tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg gcgatttgca
     3481 caccacatta gaagaggtat gccattggat gatccagctg cttttaccca tcttgatcag
     3541 ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg caattgcttc
     3601 tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga ttatcgggcc
     3661 gcttgccggc atcgtatatg aacaatttgt ttctagattc catgtgaaat tcaaggctgg
     3721 atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta tttgtataat
     3781 tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg cctccgttgt
     3841 gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag aacctctaaa
     3901 agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag gaatcgaatt
     3961 ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg gacctggttt
     4021 agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa atgcgagtgg
     4081 agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat ttgtcttgct
     4141 gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt tgatatttca
     4201 atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg tgatttttgc
     4261 caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca gtacatttgt
     4321 gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag agaacaaaac
     4381 ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca tttttgcttg
     4441 cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga aattagcagc
     4501 agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata accaagtaaa
     4561 tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag tacctcaaag
     4621 tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac gaataaaaga
     4681 agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca cagaacaaaa
     4741 aacgtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct cacaaactat
     4801 gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg ttatctcaaa
     4861 agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac aaagagaact
     4921 agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg aacattgggt
     4981 agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat ttatcgccca
     5041 gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct atccaaaaaa
     5101 tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat aatcttgaag
     5161 tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca tttgtcattg
     5221 aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa agtaatgact
     5281 tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg acagagccgt
     5341 tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt tatgcagata
     5401 tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg aaccagggat
     5461 acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta ttagaattag
     5521 aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg aattattttt
     5581 ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg gaagaattga
     5641 tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg atgctgacat
     5701 tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa tgagacatat aatagtctgg
     5761 caaacaaaga attactacag acagctaaac gattgttttc acggatcgct tctgagacaa
     5821 aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat gacttcacga
     5881 actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct tatttagtaa
     5941 aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa gatgaaactt
     6001 tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt ttacaagaag
     6061 agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa gttgcgaatg
     6121 caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa gaagagatcg
     6181 cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa agcaatatca
     6241 gtattgcagg attttcgcca agtggattag cttctactag catgttggaa ctacgattga
     6301 aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat ttaaaaaaaa
     6361 tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca ggatacggtg
     6421 gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag ctaaaagaag
     6481 ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc aataacgagt
     6541 ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc aatcaattac
     6601 tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa acgattcgca
     6661 gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt catcgccggc
     6721 tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg gggttattcc
     6781 atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg aaacatccgt
     6841 tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta gttatgctgg
     6901 cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt ggagcaatca
     6961 tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc gtttatcagt
     7021 tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat gatacagcta
     7081 ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc gttgaccgag
     7141 atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca atcaatgatc
     7201 gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc acatgggact
     7261 gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc tgatggtgtg
     7321 gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt ggctttaaaa
     7381 aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga catcgaccaa
     7441 gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc aaatgatttt tgaacatgga
     7501 catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat aacttcaacc
     7561 aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg gatgcaagct
     7621 tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt gacaaggttt
     7681 ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag atagtgccca
     7741 tacctatgaa taataaaacg atactccata taccaagtgt caagatacca ttcatgaaag
     7801 taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca ttttgaaaat
     7861 caacataaga agttggcaag taatgactga ttgattctat ccatccaatc gaagaaaaca
     7921 agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact agagaatgtt
     7981 caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac aacaacagga
     8041 acagaaagct ctggaaaaga tactcataca tcggcaggat tcgtatctcc tgaccaatga
     8101 tcgttcctac aggataacgg aagtctccca aaccattttt gattattact agtatagaga
     8161 cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg gtaaaacgga
     8221 tgctatgttt ctttaccata ctatccggta atagattgac tagagagatt acctgctttc
     8281 ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa atcaaaaaac
     8341 tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagagaga tgcgctggca
     8401 agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc aactcgacac
     8461 gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt cgatccagta
     8521 tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct gtgttttttg
     8581 catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca aattctttct
     8641 cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag ttatgattca
     8701 gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat gcaaagagca
     8761 cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga ttgcgttcaa
     8821 atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta aaatctcttc
     8881 catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt tagaacaaat
     8941 cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct ctttctgcca
     9001 ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt tcaacggttc
     9061 atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga catgttgttt
     9121 cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct tcaatcgttg
     9181 gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag tcaaataatc
     9241 aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga aagaccggcg
     9301 ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat aagttgttgg
     9361 cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg gggcaatcaa
     9421 agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac gttttttacc
     9481 acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa tataaaattt
     9541 aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta ttgtttgcat
     9601 attataaaaa aattaaaata acagttttat ttaaaattga agttttctga taccgaaatc
     9661 tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa agtacgacta
     9721 tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg agagacaaaa
     9781 tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa aaattaggca
     9841 gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg ggtgtattaa
     9901 cggctctatt tatcccagat ggttatttac caaatgaggc tttcgctacc atggtaagcc
     9961 caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa gtaattgctg
    10021 gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc ggaacagata
    10081 ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc atcaagaaat
    10141 ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc aataattttt
    10201 cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt ggtccagttg
    10261 tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct gcccatttga
    10321 ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat aatgcaatca
    10381 atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga aaatctattc
    10441 tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct tttactttgt
    10501 ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac tttttaggcg
    10561 ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc ttatcagtta
    10621 taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga ctaagagcgc
    10681 cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat tcttaccttc
    10741 ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct gcagtaattt
    10801 tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa gagacaaagg
    10861 cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca ttctctggcg
    10921 tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg ggcgcttcga
    10981 tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat caagcaatca
    11041 gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta caagaaagag
    11101 caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg aattcacctc
    11161 gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt tctgaaattg
    11221 agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa gagattctac
    11281 ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc aaagatatat
    11341 tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat ttaaaagatg
    11401 aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag atggctgcac
    11461 ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac gaatcgattg
    11521 cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt tgccattcga
    11581 catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct attgcggtca
    11641 gatgcttgta gaagcagggt gcgttgatga ggaatatatc gatgccatgg tagatagaga
    11701 caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa cagatgaagc
    11761 aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg gtgtagattt
    11821 tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag gagtcggaga
    11881 tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg acaatgttgt
    11941 ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa ttgcttaagg
    12001 agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag gatttattgg
    12061 agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc acacgatcat
    12121 tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat caaaagaaaa
    12181 aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa aagtaatcca
    12241 agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct taccttttat
    12301 tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca caacatcttt
    12361 agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa aagaagtgta
    12421 taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa atgcagcagt
    12481 cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag tggaaccttt
    12541 caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat taaaaggggt
    12601 tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg tcaatactgg
    12661 tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg atgaagcaat
    12721 gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag gaagtttgct
    12781 gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa aaatcattca
    12841 tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga caccattacg
    12901 taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag aacataactt
    12961 gacttgccca catttgacgg caacaatggg gattattttt aattactatg atcctgaaga
    13021 tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac aattgattca
    13081 agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa acgtgaaccg
    13141 ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac gaaatttttg
    13201 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
    13261 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
    13321 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
    13381 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
    13441 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
    13501 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
    13561 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
    13621 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
    13681 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
    13741 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
    13801 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
    13861 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
    13921 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
    13981 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
    14041 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
    14101 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
    14161 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
    14221 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
    14281 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
    14341 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
    14401 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
    14461 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
    14521 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
    14581 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
    14641 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
    14701 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
    14761 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
    14821 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
    14881 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
    14941 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
    15001 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
    15061 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
    15121 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
    15181 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
    15241 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
    15301 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
    15361 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
    15421 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
    15481 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
    15541 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
    15601 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
    15661 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
    15721 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
    15781 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
    15841 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
    15901 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
    15961 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
    16021 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
    16081 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
    16141 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
    16201 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
    16261 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
    16321 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
    16381 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
    16441 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
    16501 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
    16561 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
    16621 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
    16681 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
    16741 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
    16801 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
    16861 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
    16921 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
    16981 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
    17041 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
    17101 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
    17161 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
    17221 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
    17281 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
    17341 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
    17401 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
    17461 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
    17521 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
    17581 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
    17641 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
    17701 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
    17761 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
    17821 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
    17881 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
    17941 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
    18001 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
    18061 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
    18121 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
    18181 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
    18241 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
    18301 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
    18361 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
    18421 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
    18481 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
    18541 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
    18601 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
    18661 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
    18721 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
    18781 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
    18841 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
    18901 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
    18961 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
    19021 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
    19081 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
    19141 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
    19201 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
    19261 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
    19321 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
    19381 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
    19441 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
    19501 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
    19561 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
    19621 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
    19681 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
    19741 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
    19801 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
    19861 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
    19921 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
    19981 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
    20041 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
    20101 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
    20161 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
    20221 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
    20281 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
    20341 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
    20401 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
    20461 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
    20521 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
    20581 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
    20641 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
    20701 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
    20761 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
    20821 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
    20881 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
    20941 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
    21001 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
    21061 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
    21121 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
    21181 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
    21241 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
    21301 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
    21361 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
    21421 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
    21481 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
    21541 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
    21601 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
    21661 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
    21721 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
    21781 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
    21841 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
    21901 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
    21961 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
    22021 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
    22081 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
    22141 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
    22201 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
    22261 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
    22321 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
    22381 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
    22441 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
    22501 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
    22561 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
    22621 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
    22681 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
    22741 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
    22801 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
    22861 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
    22921 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
    22981 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
    23041 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
    23101 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
    23161 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    23221 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    23281 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    23341 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    23401 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    23461 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    23521 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    23581 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    23641 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    23701 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    23761 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    23821 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    23881 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    23941 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    24001 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    24061 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    24121 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    24181 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    24241 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    24301 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    24361 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    24421 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    24481 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    24541 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    24601 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    24661 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    24721 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    24781 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    24841 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    24901 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    24961 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    25021 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    25081 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    25141 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    25201 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    25261 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    25321 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    25381 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    25441 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    25501 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    25561 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    25621 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    25681 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    25741 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    25801 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    25861 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    25921 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    25981 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    26041 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    26101 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    26161 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    26221 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    26281 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    26341 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    26401 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    26461 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    26521 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    26581 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    26641 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    26701 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    26761 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    26821 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    26881 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    26941 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    27001 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    27061 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    27121 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    27181 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    27241 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    27301 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    27361 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    27421 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    27481 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    27541 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    27601 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    27661 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    27721 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    27781 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    27841 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    27901 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    27961 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    28021 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    28081 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    28141 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    28201 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    28261 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    28321 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    28381 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    28441 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    28501 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    28561 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    28621 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    28681 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    28741 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    28801 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    28861 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    28921 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    28981 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    29041 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    29101 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    29161 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    29221 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    29281 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    29341 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    29401 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    29461 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    29521 gtaaatgcta taaatccagt gatagttaaa gaaa
//
LOCUS       DAXWLH010000014        29107 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXWLH010000014 DAXWLH010000000
VERSION     DAXWLH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.014"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..1030)
                     /locus_tag="ACGE36_000449"
     CDS             complement(<1..1030)
                     /locus_tag="ACGE36_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8382874.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="ACGE36_000450"
     CDS             complement(1112..1801)
                     /locus_tag="ACGE36_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8382875.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACGE36_000451"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACGE36_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFF8382876.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACGE36_000452"
     CDS             complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACGE36_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFF8382877.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACGE36_000453"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACGE36_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFF8382878.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACGE36_000454"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACGE36_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFF8382879.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACGE36_000455"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACGE36_000455"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFF8382880.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACGE36_000456"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACGE36_000456"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFF8382881.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7543)
                     /locus_tag="ACGE36_000457"
                     /pseudo
     CDS             complement(6443..7543)
                     /locus_tag="ACGE36_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7635..8522)
                     /locus_tag="ACGE36_000458"
     CDS             complement(7635..8522)
                     /locus_tag="ACGE36_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8382882.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACGE36_000459"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACGE36_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFF8382883.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACGE36_000460"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACGE36_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFF8382884.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACGE36_000461"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACGE36_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8382885.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /gene="cbpA"
                     /locus_tag="ACGE36_000462"
     CDS             9753..10397
                     /gene="cbpA"
                     /locus_tag="ACGE36_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFF8382886.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="ACGE36_000463"
     CDS             complement(10453..10956)
                     /locus_tag="ACGE36_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8382887.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="ACGE36_000464"
     CDS             11553..12518
                     /locus_tag="ACGE36_000464"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8382888.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="ACGE36_000465"
     CDS             complement(12617..13789)
                     /locus_tag="ACGE36_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8382889.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="ACGE36_000466"
     CDS             complement(13776..14921)
                     /locus_tag="ACGE36_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8382890.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="ACGE36_000467"
     CDS             complement(14935..15867)
                     /locus_tag="ACGE36_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382891.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="ACGE36_000468"
     CDS             15984..17063
                     /locus_tag="ACGE36_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8382892.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="ACGE36_000469"
     CDS             17060..17689
                     /locus_tag="ACGE36_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8382893.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="ACGE36_000470"
     CDS             complement(17799..18026)
                     /locus_tag="ACGE36_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8382894.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="ACGE36_000471"
     CDS             complement(18129..20717)
                     /locus_tag="ACGE36_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFF8382895.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="ACGE36_000472"
     CDS             complement(20737..21396)
                     /locus_tag="ACGE36_000472"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8382896.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACGE36_000473"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACGE36_000473"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFF8382897.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="ACGE36_000474"
     CDS             complement(22169..23035)
                     /locus_tag="ACGE36_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8382898.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="ACGE36_000475"
     CDS             23144..23785
                     /locus_tag="ACGE36_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFF8382899.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACGE36_000476"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACGE36_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFF8382900.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="ACGE36_000477"
     CDS             complement(25226..26125)
                     /locus_tag="ACGE36_000477"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8382901.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKSYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="ACGE36_000478"
     CDS             complement(26127..26924)
                     /locus_tag="ACGE36_000478"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFF8382902.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="ACGE36_000479"
     CDS             complement(26902..27606)
                     /locus_tag="ACGE36_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8382903.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="ACGE36_000480"
     CDS             27737..28312
                     /locus_tag="ACGE36_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFF8382904.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="ACGE36_000481"
     CDS             28430..29107
                     /locus_tag="ACGE36_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFF8382905.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6168 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agactttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaa
//
LOCUS       DAXWLH010000015        28535 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXWLH010000015 DAXWLH010000000
VERSION     DAXWLH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.015"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            14..294
                     /locus_tag="ACGE36_000482"
                     /pseudo
     CDS             14..294
                     /locus_tag="ACGE36_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(372..905)
                     /locus_tag="ACGE36_000483"
     CDS             complement(372..905)
                     /locus_tag="ACGE36_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8382906.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(999..2177)
                     /gene="mutY"
                     /locus_tag="ACGE36_000484"
     CDS             complement(999..2177)
                     /gene="mutY"
                     /locus_tag="ACGE36_000484"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFF8382907.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2170..2967)
                     /gene="recX"
                     /locus_tag="ACGE36_000485"
     CDS             complement(2170..2967)
                     /gene="recX"
                     /locus_tag="ACGE36_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFF8382908.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3074..4456
                     /gene="rlmD"
                     /locus_tag="ACGE36_000486"
     CDS             3074..4456
                     /gene="rlmD"
                     /locus_tag="ACGE36_000486"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8382909.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4630..5208)
                     /locus_tag="ACGE36_000487"
     CDS             complement(4630..5208)
                     /locus_tag="ACGE36_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFF8382910.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5530..6000
                     /locus_tag="ACGE36_000488"
     CDS             5530..6000
                     /locus_tag="ACGE36_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFF8382911.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6108..7334)
                     /locus_tag="ACGE36_000489"
     CDS             complement(6108..7334)
                     /locus_tag="ACGE36_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8382912.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8088..8288)
                     /locus_tag="ACGE36_000490"
     CDS             complement(8088..8288)
                     /locus_tag="ACGE36_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8382913.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8422..9030)
                     /locus_tag="ACGE36_000491"
     CDS             complement(8422..9030)
                     /locus_tag="ACGE36_000491"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFF8382914.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9115..9585)
                     /locus_tag="ACGE36_000492"
     CDS             complement(9115..9585)
                     /locus_tag="ACGE36_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFF8382915.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9633..10547)
                     /locus_tag="ACGE36_000493"
     CDS             complement(9633..10547)
                     /locus_tag="ACGE36_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8382916.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10693..11496
                     /locus_tag="ACGE36_000494"
     CDS             10693..11496
                     /locus_tag="ACGE36_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFF8382917.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11674..12621
                     /locus_tag="ACGE36_000495"
     CDS             11674..12621
                     /locus_tag="ACGE36_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFF8382918.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12689..12982)
                     /locus_tag="ACGE36_000496"
     CDS             complement(12689..12982)
                     /locus_tag="ACGE36_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFF8382919.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13010..13675)
                     /locus_tag="ACGE36_000497"
     CDS             complement(13010..13675)
                     /locus_tag="ACGE36_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFF8382920.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13815..14447)
                     /locus_tag="ACGE36_000498"
     CDS             complement(13815..14447)
                     /locus_tag="ACGE36_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8382921.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14454..15521)
                     /gene="liaS"
                     /locus_tag="ACGE36_000499"
     CDS             complement(14454..15521)
                     /gene="liaS"
                     /locus_tag="ACGE36_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFF8382922.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(15518..16249)
                     /gene="liaF"
                     /locus_tag="ACGE36_000500"
     CDS             complement(15518..16249)
                     /gene="liaF"
                     /locus_tag="ACGE36_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFF8382923.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(16417..16896)
                     /gene="greA"
                     /locus_tag="ACGE36_000501"
     CDS             complement(16417..16896)
                     /gene="greA"
                     /locus_tag="ACGE36_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFF8382924.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17032..18207)
                     /gene="mltG"
                     /locus_tag="ACGE36_000502"
     CDS             complement(17032..18207)
                     /gene="mltG"
                     /locus_tag="ACGE36_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFF8382925.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            18372..19532
                     /locus_tag="ACGE36_000503"
     CDS             18372..19532
                     /locus_tag="ACGE36_000503"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8382926.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
     gene            complement(19815..21128)
                     /locus_tag="ACGE36_000504"
     CDS             complement(19815..21128)
                     /locus_tag="ACGE36_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HFF8382927.1"
                     /translation="MATLHEKKVQFNSKLTISNTGGNLSTDSGLVLVKEFMESLNFSD
                     LSKQYLGIEDKRLYHIHDNFSLMEQLIYQNIAGYSTDSSANILKQDPIFKVILDKSRL
                     ASQSSISRFWDRISEENISQLQDLNQAMIDKVRSARNTTEMIFDLDSTHSDTYGDQEN
                     TDYNAHYRTNGYHPLVAFDGLTGDFLKAELRSGNVYTSNGVGTFMEPLFEHYNQVVPV
                     SNILVRGDSGFATPELYDLCEAYDSFFVIRLKANRNLSKIAESFIQIDDNHPWDKKEV
                     VYSSTSYQAKSWSKERRVCIKSTREADELLFRHEYIVTNYSNNVSAETVFRTYCKRGT
                     MENFIKEAKNGFYFDKTDSPSFLENHARMMVSLLAYNIVNFMRTLCFTQGSASMQVDT
                     IRLKLFKVAGKLVRTGRRLLLKLSSYHLHQGLFYQVLGNIQQLCW"
     gene            21446..21877
                     /gene="mraZ"
                     /locus_tag="ACGE36_000505"
     CDS             21446..21877
                     /gene="mraZ"
                     /locus_tag="ACGE36_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFF8382928.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            21898..22857
                     /gene="rsmH"
                     /locus_tag="ACGE36_000506"
     CDS             21898..22857
                     /gene="rsmH"
                     /locus_tag="ACGE36_000506"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFF8382929.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            22862..23263
                     /gene="ftsL"
                     /locus_tag="ACGE36_000507"
     CDS             22862..23263
                     /gene="ftsL"
                     /locus_tag="ACGE36_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8382930.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23264..25456
                     /locus_tag="ACGE36_000508"
     CDS             23264..25456
                     /locus_tag="ACGE36_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8382931.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            25482..26444
                     /gene="mraY"
                     /locus_tag="ACGE36_000509"
     CDS             25482..26444
                     /gene="mraY"
                     /locus_tag="ACGE36_000509"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFF8382932.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            26458..27819
                     /gene="murD"
                     /locus_tag="ACGE36_000510"
     CDS             26458..27819
                     /gene="murD"
                     /locus_tag="ACGE36_000510"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFF8382933.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            27845..>28535
                     /locus_tag="ACGE36_000511"
     CDS             27845..>28535
                     /locus_tag="ACGE36_000511"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8382934.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEE"
BASE COUNT         8875 a         5515 c         5103 g         9042 t
ORIGIN      
        1 aggtggctag accatgaaag ttgtacaaac aaaagataca atgagcaata tttacttaga
       61 tgctgtacgg atcaggcatc aagtttttgt agtcgaacag ggtatgcctt tgtctcgaga
      121 aatagacaaa gatgaagccc attgtatcca cttcgtcctg tactcagata aaaaagaacc
      181 gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa atgaaattac agcggatggc
      241 gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt ataaaaattt
      301 atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg gcttactgtt
      361 cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat atcaatatag
      421 ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt cacattttct
      481 tttaggatga agtcaatatc gttcggataa ttcatcattt tgctgtgcat ctcatattca
      541 tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa gtcatagtcg
      601 atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca ataataggaa
      661 accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg aaaataaaca
      721 atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa tgtgtgatca
      781 ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg ccacgttcga
      841 tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc ttttggaact
      901 cccatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt tcattacacc
      961 gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt cctattgttt
     1021 cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac gaccgaatca
     1081 aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg ttctgctccc
     1141 tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa aatatgagta
     1201 acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa tgggagattt
     1261 tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt ttcccattct
     1321 tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt ccacatattc
     1381 gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg attttgaaga
     1441 gccgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac aggaaaactc
     1501 gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt ctcacacttt
     1561 ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt gaattcacct
     1621 ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct gctagaagct
     1681 tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac gttgccgtca
     1741 accgcgggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt atatggtcct
     1801 attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc aaattctgac
     1861 atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc aaggccctcc
     1921 catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt aggaaaccat
     1981 tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg aagcatgatc
     2041 tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt tcttttttct
     2101 tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc tgtccatttt
     2161 tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga tcatatccaa
     2221 atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt taggttcaaa
     2281 ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat attctatttc
     2341 ttgatctgct tcttgcggaa gatcagccat cgcttgttgg atcacttctt gagaaaaacc
     2401 tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt ttccgcgatt
     2461 ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt caaatgtata
     2521 ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca tctgctgcgc
     2581 taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac tttccccata
     2641 gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat ttcgatcttc
     2701 ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc gcaactgata
     2761 acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct tcttgatctc
     2821 ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta ctaatacatc
     2881 ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag ggcctttgcc
     2941 tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat tctctatcat
     3001 accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag aaaaaagaaa
     3061 aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc ctttgaaaat
     3121 caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga tcatatttgt
     3181 tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa ctcctcgcta
     3241 tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag tggcaccatg
     3301 tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc aagctcaatt
     3361 ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag caggctttcg
     3421 atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata gaaacaaagc
     3481 acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt tatacgaagc
     3541 aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc gaacacaaaa
     3601 agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct atgatgaacg
     3661 gacaaacagt ggaatcttcc gcacaatcat ggttagaatc ggtgttaaaa ctggggagtt
     3721 acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa tgatctgtga
     3781 gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg cacaaaaaac
     3841 gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca ttgaagaaca
     3901 aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca atcctcagca
     3961 gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta atgaaacgat
     4021 cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac aagccaaaga
     4081 agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa atgcggaaag
     4141 attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc tgcctaaatg
     4201 gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta cgggtttgga
     4261 taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt atatctcctg
     4321 taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca agattgaata
     4381 tttacagtct gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg tgaagatgac
     4441 gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc aagtaataag
     4501 aacagccaaa caaaaatagc tcttcatgtt ttatgaatac cgtacttgtg accgaagagt
     4561 taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg tcacagccct
     4621 ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg tcgtatcgct
     4681 gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa gtgtgatcgt
     4741 ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga tcaattcaaa
     4801 atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt agtaatccca
     4861 agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc ctggattaac
     4921 catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc cgctgactcc
     4981 tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac tttgcggttc
     5041 ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc ctaggtatgc
     5101 acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa aaccgaacaa
     5161 ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt agaaacctcc
     5221 ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt atgcttttat
     5281 aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa cagaagtaga
     5341 cgagaaaagt acaaatagaa aacatgagaa ccaaatcata tgatcttttt caaaaacatc
     5401 attcatataa acaaatgttc aactttttaa taacaacaat catcaatttt tttcttactt
     5461 cacaaactaa atacaggtct tgctgtgagc cttcatggta aactactatt atagaaagga
     5521 gttagtaaaa tgaagatgat aaaagatatt cgtttttggg tttgtgttat aggaattgtc
     5581 atattaggct ttttatcagg gttgcttagc ggaaatcccg gagaatatta ctattctttg
     5641 caacttccac catttgcacc acctagctgg atatttgggc cgatgtggac cttactgtat
     5701 atattgatgg gtatttctct ttatttgtta cttaatcaca acaacaaaaa acagcgaaac
     5761 aatcttgtcg gtctttttgt gattcagttc atttttaatt ttatatggtc tgctctattt
     5821 tttaatttaa gaaatatttt cattgcagca atcgatatta cacttttagt tattttcttg
     5881 tcagtgctta tgtatcagtt gtggctacat catcgcctgg caatgtggct catgattcct
     5941 tattatttat gggtactatt cgcaactcta ttgaattatt caatttattt tttaaactaa
     6001 gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa agtgccacct aagttagatt
     6061 ttttgtctaa tttaggtggc actctttatt aaccaaacta tttttttcta gtacccactt
     6121 gttgttccat actcattatt ctggtcataa ccataaccat tttgttcctg tccagcgtaa
     6181 ggatcataac tttgagtatc ttgacttggt gtatagtact gacttgtatc ttctgtttgc
     6241 tgataatagg tattcggatc ttggtactga tcatatgtgt tttctgaata ggaagaataa
     6301 tcggtgtacc cttcactatt cgatccatca ccgtttgtcg aatcgccatc ttccgaatca
     6361 tcaaagaaca tcattgaagc attctcattt ttcaaattag gtaatgtatc gcttgttttt
     6421 atatttaatt gtttcttcag ttcattttgg atcgaaagaa gatcattttt tcctaaaatt
     6481 tgatagtaga tatcattgat catttgtcct tcgccttgca actggtcttg tttgattgtt
     6541 tcaaacgcag gggtgtagtt cgtcgctaca tcaagcatgt catcccaatc taagtccgtc
     6601 gtcacattgt tctcaactgc ttttaaaatt tttctataat tgcttacgcc atctaaactt
     6661 aaaactttat tgacgatttt cgtcactact tcccgctgac gacgttgtcg accgatatcg
     6721 ccttctggat cttcgtgtcg cattcgagaa taggctaaac ctttttctcc attcaatgtt
     6781 tgttcaccta ctggtacatg gacaccatcc aaagtaaagt caattttatt attcactttg
     6841 atgccaccga ccgcattgat caaatcttcc atgccatcca aattgatcgt tacataatga
     6901 tcaataggaa tatcgagcag ttgctcaatt gaatccatgg ccatttcgac accaccaaat
     6961 gcataggcat gatttaattt atctaccgtt ccataaccta cgatattcgt ataaatatct
     7021 cggtccaaac tgatgatcgt tgattttttt tgtttcggat taactgttac taccatcatc
     7081 gtatcagaac gtccttgttc cgttcttcct aatccaccgg tatctaaacc taatagcaaa
     7141 accgaaaaag gttctttgtc atcaatgctt actctctcag aacgtttgga ggatattcga
     7201 ttagagtttt tagaaatttt gtttactgtt tggctcgctt ctcccataaa tctgattccg
     7261 tatgctgata atccggcaat cagcaaaaca aataccccta acaaggttaa tagaactttt
     7321 tgccatcgct tcataataca cctctgtttc acattttcta aaatattgtc tcatataact
     7381 gttcaatcag caaattcact ttttaatcat aagaaaactt taaaaaacat ttttgtgtaa
     7441 cgattactat tttttaaaat ttctcaaaat tcttaactat cataccatac cgtgaaaaag
     7501 atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttacg tattttttcc
     7561 agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga tcgcacatac
     7621 ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga tttcacgaaa
     7681 atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt atttccagct
     7741 cactgcatgg ctcttaaaat gactcgaact agtgatttca ttaccatcat cttttaatgg
     7801 atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga attgacatgg
     7861 taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc caaaaactga
     7921 aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa ttagttagga
     7981 catgtcaagt gaacaaatgc atttacttag ccaaaaaatc taaaaacttc ataaatagac
     8041 aaaaagacac gactctctaa gaagtcgtgt ctttattcaa caaattatta taatttagtc
     8101 acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg tgaattctac ttcttgacct
     8161 tcttctaggc tcttgaagcc gtctccttga attgctgaga aatgaacaaa tacgtcatct
     8221 ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt tgaaccattt aaccgttccg
     8281 tgttccataa atgtatagcc tccaaaaaag ttcttgcaag taaccttgca agaacattat
     8341 acccaattta gaaaacgcta gcaagctttc gctcttttat tttccaaatt atttttaaga
     8401 ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa cgtttgttta cttcatccca
     8461 attaataaca ttccagaatg ctgcaatata atctggtcgg acatttttat atttcaaata
     8521 ataagcatgt tcccaaacgt ccaaacctag aattggtgtc tttccttcca tcaatggaga
     8581 atcttgattt gcagtagagg taatagctaa ttttccattt tccattacaa gccaagccca
     8641 tccagaacca aatcgtccag cggctgcttt tttgaattct tctttaaaag aagaaaaatc
     8701 accaaaagct tcattaatcg cttcttttat ttctcctgta ggttcgccac cagcatttgg
     8761 tgccatgatt tcccagaaaa atgaatggtt agcatgtccg ccaccattgt tacgtacagc
     8821 tgtcttgata tctgttggaa tagcgtccat atcagataat aattcttcta ttgttttttc
     8881 gcctaattct gggtatttct caattgctgc atttaaattc gtcacataag tattgtgatg
     8941 cttatcatga tgcagatgca tcgtttcttc gtcaatataa ggttccaatg cgtcatatgc
     9001 ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc ctcctcaatg ttttctttat
     9061 aatgtctaca ttacaaagag taacaaagag aaggaaattc ttcaattgtt atgcctagtc
     9121 gtctttttca ctttgaccta cttcttcttc aaaaacctca gtttctttaa aaataaacaa
     9181 cttgctgaaa atatagttcg ctaggataac aataatttga gtgatgattt tcgccacata
     9241 ctcattgctg ttcataacat tgatcaacaa taccattgcc cccatgtcca atccgtaaga
     9301 aaggatacgg tagaagaaga acttaccgca ttcaataagt gcatgtgtaa aattcggtga
     9361 ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca aacaatacag aacaaaacca
     9421 cgaaatagaa ttggataagg ccatacttaa atcaaaatat tggcgtgcga gagcaaaact
     9481 aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga tagactagta tctcccaata
     9541 tccttttgct tctaaatatc cttttaactg tcgatactgc ttcatgagcc aacctacctt
     9601 attaaaaatt cttatttatc atactctata tttcaataac tttcatcttt atttaaaaaa
     9661 attaattttt tttgatcctt tgcctttgtc tctgcttgga tcgtcacatg tgtaatacca
     9721 tattttaatt ttaataaaga aattatacga ggatatatct tttcaatctc gcttagtagc
     9781 tgatcttcaa aatcaatatg tccgtcgaaa ataattatct tttcgctata ctgccaaata
     9841 tggacatgat gaacattctt taccccatcg attgcttcaa tttcttgttc cattttttca
     9901 tagtcaatag caggtgcact ttgtatcaaa atagcggcag cttttttgat caccattatc
     9961 gcttctatca atatatatat cgaaatgatc agtgtaataa ctggatcgat ccagtagata
    10021 tcgaaaaaac ggatcaaaat tgctccgata atcacactta ccgaagacag tgtatcactt
    10081 aacaaatgca aataactaga ttttatattc aaactttcat ctgcttcttg tcgtaataat
    10141 aatactgaga acaaattcgc gagcaatcca ataactgcta ctgttagcat caaatgacta
    10201 tttatctttt ctggtgaagt gaaccgcttg aatgcttcaa cggttaaaaa caaggataat
    10261 atgatcaata tgaatgagtt gatgaaagca ctcaatattt ctaaacgctg gtagccatac
    10321 gtacgttttt tatttgcttc ttttcgggcc attttttgtg ccccgtaaga aaaaataatt
    10381 gccaatgtat cactaaggtt atggaaagca tctgaaacaa gagacaaact ccctgacata
    10441 atccctccaa taaattctga taaagtgatc accgcattca aaaccactac tccaaatagt
    10501 ttttttgatg atctctgtgg ctgtgtaggc actattcctt cctccatttt atttatattg
    10561 gatagtcttt agacaagaat aacatttttt tgatatttca ccaatcattc agatgtttcc
    10621 ctaaagcact tgtatttcac ttgatacttc gctagaattg caagtgatga cactactaga
    10681 tgaggagtac ttatgacaac aaaacaatta attatcagtt cctttatacg attcaaagac
    10741 ctaaaggagg cgagaaatgt cccattttgg aaagccattg tctatctttt agttttgagt
    10801 ataattatgg ctttgccaat ctcttttcaa gtttttcaag tcttaaataa cattcaattg
    10861 gatgggcaaa agattgctac tcgcattcct gattttacaa tccaaaacgg gcagcttcaa
    10921 accgaagaaa aagaaggatt catttaccaa acaaactcca ttatcttcac atttgatcca
    10981 gagggaaaac gaacagaaca ggacatctct actgatttga tggggaattt tgttagtgtc
    11041 gggatgttaa aaaacaaact tatcatagct cttcctaata caggcacgac ttctgctttg
    11101 ttgaacaata accaattaga acttccctac accaatgaat ctttaaaaaa cttaacaggt
    11161 aagcagctcc gttctttttt aagtgaagca tctataccac tttggataaa agtcctgaca
    11221 tttatatttt ctatttatcc tagttttctg aatctgctga tcactttgct atttaccaac
    11281 attgctgctt ttctctatgc acgctttcga ctaacaaaag ctacttttct tgattgttta
    11341 aaaacattga tctattcaat ttcattgcct actataattg caacaatctt gatggtcttt
    11401 cttccttctt ttgacactag cgccttcatt gcttttgcag gaatctttat ttttgctcaa
    11461 gctgtcaaag gatgggcaaa aatatcgatc aattaagaaa taaagacaac tcgttatttc
    11521 tacttaaata gaccctttta gaggttgtga catttttttg tcacagcccc ttttttattt
    11581 gaaaaatatt tctttctcaa aaaacaagca aggcgcttca aagttcttct tgtatgcgct
    11641 acacttagag taaagaaagg cgggaaatta actatgtcag aaagaatcat ttatgcagat
    11701 cgtccatttc gtgatgaatt tatcgaagaa atccatacta tagcgcctgg ttatatcttt
    11761 aaaacagaat tacaaccaga ggatctacaa aatgtagaga tcagtttggg ctggaactca
    11821 gactaccaag aaaaactatt agcctcttct accttgaaat gggtccaatc tatctcagct
    11881 ggtgttgatc agctacccct tgagactttt tcaaagaaac atattttact ttcaaatggt
    11941 agtggcatcc acagtgaatc gattacggag catgtcttag gaatcatcct tgggtattct
    12001 cgtggtcttt ttcaagcaca aaaggcacag agtgacatga aatggttagg ttcatcggtc
    12061 cattaccaat ctatccaagg gaaaaaaata ctaatcattg gaactggcca tattggaaaa
    12121 atgattgcaa aaaaatgcaa agcattagac atgaactgct atggaatcaa tacatccggt
    12181 catccagcag aaggaatgga aaagactttt tctttaaatc aactaaagga agttcttcct
    12241 gaagtagatt tcgtagtcaa tatcttacct ctaacggagg aaacaactgg gctctttgat
    12301 cagcatatgt ttgaacactt tgatccaaaa tctgtattca tcaatgttgg ccgtggtgct
    12361 tctgttaaga cacaagatct ggtgcaggct ttaaataacc agcaactttc ctttgcagca
    12421 ttagacgttt ttgaggaaga gcctcttcca caagatcatc ctctttggaa aatggacaat
    12481 gtactgatta cttcccacat tgcaggtttg acacctgatt ttcaaaagaa attaatggct
    12541 atttttttaa ccaatctgaa aagctacttt gccacccatg agcttcaaac caatcaagta
    12601 aaattgtctg caggatatta atagctgaaa acaatatttt caaaagaaaa aagagaaggt
    12661 gacaaaaatt gtgtcaccct ctcttttttt attgtttttt aaagtgatag gttggctcct
    12721 ccaggttggg atcataatca acaagtaaat catatccttt aaagaaccat tcatctgctt
    12781 cctcgataaa aaaaagtgtc ccatccattt ccactttctt tacaggatgt tctggctgtt
    12841 caactgacat tccaatcgaa aaaccatcat gaacctctgt tttgccgtag acttttccaa
    12901 aaaaacgtat tccgtaagtt tctggcagat caatttctga gtcaaaccat ttttgtgctt
    12961 ttggcgttac cgttaatttc atcccaacaa cctactttct cttttctttc tattgattca
    13021 tcttatcgtc tagaatatcc acatcgcctg tctctccaat aaccagtaga ttatcatttt
    13081 cacgaacata ttcatctgct gcaggagaag cgataaccgt ttgatctact cttcgtatgg
    13141 cgactactgt taagtcaaaa cgttgcctga aatttaattc agctaatgtt ttgttgaaaa
    13201 atttaggatt ggagactcgg atttctgcta aagaatattt atctgacagc tctaagtagt
    13261 ctagaatatt ctttgatact aagttatgtg caatccgtat ccccatgtct cgctcagggt
    13321 gtacgacacg atctgctccg atcttctcta gtacacgggc atggtactca ttttgggctt
    13381 ttgctaaaat attcggtacg cccatttcct ttaccatcag agtaactaag atgctggctt
    13441 ggatatcttc tccaatagcg acaattacat gatcgaaatt tcttatacct aatgaacgaa
    13501 gtgtcatctc atcctgtgca tttgccacaa cagcatgggt ggctatattc atatattcat
    13561 tgactcgatc ttcattactg tcaatagcca gtacttcttg tccagcttcg atcagtgtac
    13621 ggcagatgct tccgccaaat cgtccaaggc ctacaattgc aaaattttgt ttcaccattt
    13681 tcctccttta gtatcttctt ttctcagtat aacaaagcct ttccaaaaac aaaagcaagc
    13741 atcattattg taaaaaagcc aagaaaaata atagttattt tctcagcttt tgattatcct
    13801 ctttatcatc gtttttattt caccaaacca tgtttaaacg catagatagc tgcttgagtt
    13861 cggtcttcta cctctaactt agctaatata ttcgacacat gcgttttgac tgttttcagt
    13921 gtgataaaca attcatccgc gatttcctga ttacttttcc cttcagaaat cagcatcaaa
    13981 atttcatttt ctcgattcgt taattcttca tgcaatactg gatcatttcg ccgactcatt
    14041 ttttccatca tttttgttgt cacttcaggt tctagtactc gctcgccgcg ttgcgtagcc
    14101 cttatagcat cagctatttc atgtgctgta gacgtcttca acaaatatcc tgctgctcct
    14161 gcttcaattg caggataaac tttttcatca tcaataaaac ttgtgacgat aatgattttg
    14221 gcttttggcc aatcttttaa gattgctttt gttgattcga tgccatccat ttcttccatg
    14281 actagatcca ttaaaatgac atccggtcga agtgccatcg ccttttcata gccttgacgt
    14341 ccattttcag cttctccaat cacttcaatg tcttcttgga tagataaata agaagagacg
    14401 cctaagcgta ccatttcatg gtcatctact aataaaactt ttatcatctt tatctatgcc
    14461 tccttcatca aagggacctt ggtctccacg ctcgtacctt gccctttaaa actaatgatt
    14521 tttactgttc caccaatacc agctactcgt tctttgatat tgttcaatcc ataacttccc
    14581 gttttggttt cattcatatt aaatcctgtc ccgtcgtcaa tgatacgtaa taaaagattg
    14641 ttatctattt tatgcaaata gacttctaat tcattagcct tagcatgtct taatgtgttt
    14701 gataacaatt cttgaacgat tcggaacaag tgatcctcaa tggaactagt taattttaca
    14761 tcttctacct cccattttag cgaaatctgg attttattct gcaattcctt caataactgc
    14821 tcaatgcctt gttttaaact tttttcttct aaattgactg gacgcagatg aagaagcagc
    14881 gcacgcatct cagactggga agcgttgatg atctctgcca ccattttcaa ctgcttacgg
    14941 aacatttctg gtgtctcact tttttctgct tgctccgtca atgccgacat catcatcatc
    15001 gctgcaaata attgctgtga aactgaatca tgaagctcac gagctaatcg atgcctttct
    15061 aattttaaaa tctcttcttt ggtttgtcca tctacatatt gaggacggct agtcataatc
    15121 tgtaattcac tggatacttc gatcattttt tcttttattt tagtaatttc catatcaata
    15181 tctttgatgt acagatcatc cgacgcgctt gggatcggct gatgcaaaag tttggattca
    15241 taattgccag aagaaagtgc acgaagcttt tcttcgattt tcccgtattt tgccttttga
    15301 ataagtgata acaatagaaa tacgatcaat cctacaccta atgaaattgc catcaaatgg
    15361 aagataagcg gaacgtataa taaacgcgca cgcagcaatt ctccccacca atgactttgg
    15421 ttactggcat aaaaataagt gaaaagtgaa aatagaataa taaggaaagc cgcaatcccc
    15481 gaataaacag ctagcattgc tttggatatt cttcccatca tattcgtatc acctcgacat
    15541 ctccaaccaa agtattggta atgatcttca atctgcgagg attttcatca taatcactgc
    15601 tgcagatttt gatctgttcg ttttttaagg aaaactgctc ttcttcaaac aagacatttc
    15661 ctactaatgt cgcatgctct aaacgaatag ccactcccag gggtactaag atccgtgttc
    15721 tgcctatccc ttttcttaca atcacaatat tatcatcttt tggcaaaagt gtattgccca
    15781 aatcgatgat agtatcacca gaaatgatgg caatgttgat atcatcccat tcatagacgt
    15841 cgtttccaat acgttggtta ccaaataatt gctgtctttt acgctcatta ttatgtgttt
    15901 ttaattgctc tgtttggacc atcatgatct gtttttttcg ccaaaaagca ttttttgtca
    15961 aatctattcc tgaaatctct acacctttca gcccgataaa taaaacggcc aatacaacca
    16021 tcatccataa agcaggactg ttgactaagc taaaaaaaat gaccaagctc cctaagatca
    16081 attgaaaatt ttgaaattta gtacgaggat atttgcgcat agctagatat atattgaaaa
    16141 taccgaatat tactaaaagc aaaagtccag tattgtttac gatttgccaa acagcaaata
    16201 acagcagcag tgcttcgact accacaaaaa aacgccaaga actattcatc gctttttctc
    16261 cctcctgaca cattctcatt tcatcatacc tatttttttg actgttggct ataggacttt
    16321 agatgtaaga tcgttcagtg aaacctgcaa tactcaaaga gaaaagatct agagtgcgtc
    16381 atcagtaaga tgaactctct agatctttca gtcagactac gcttgttcta ctttgacgat
    16441 tttaactaac atatcgccac ctggtgtagc aatggttact tcatcatttt tacgtttgcc
    16501 aatcaatgct tgcgcgatag gagaatcatt ggaaatcttt ccagaaaacg ggtctgcttc
    16561 agcacttccg acaatcgtgt actcttcttc atctccatca ggcaactcga tgaacgtaac
    16621 tgttttaccg atcgatacct catctgcatc tacgccgtca ttatcgatga tttgtgcaaa
    16681 acggatcata ttttcaattg ttgtgattct tccttcaaca aatgcttgtt catctttagc
    16741 tgattcgtat tcggagtttt cggaaagatc tccgaaaccg cgagcgattt tgattcgttc
    16801 aatgatttct ccgcgtttaa ctgtttttag ttcttctaat tctttttcta atttttcttt
    16861 accttctaga gtcattggat aaactttctc taccatgtgt aacgtctcct tttttcgata
    16921 aaataccgca aaaggtaatc ttttgaggaa tatagtccaa aaaagattac cacgcttttc
    16981 ttaaaatgta aatgtttttc taaaaatgat caatgactag cccctgtttt tctaagaatt
    17041 atttacatat ttttgcacta gttccatatg ttcttcatac gttttagcga aataaacatt
    17101 tccagtgctc gtatctgcta cgaaataata atagtcattt tctgctggtt ctaatactgc
    17161 cttgattgcc tcttcgcttg gattgtcaaa aggaccaggc ccatagccgg tattggtata
    17221 caaattgtat ggtgaatcta cagctgtatc ttcatacgtc accaattctt tatgttctcc
    17281 taatgcatat aaaacagaga tatctgactg aagtggcata tttgccttga ttcggttaaa
    17341 gaagacttgt gcaatatttt tacgatcatt ttcttttacg ccttcttttt caactagaga
    17401 agccaaagtt aaaacttctt gtacagtcaa atttttctgt ttgatagctg aataatagtt
    17461 ttgcatgacc gtatttgttt tgttcaccat ctcaatcaca agatccttaa gcgaagtact
    17521 cgtgtagtaa tcataagtag ctgggaataa gtagccttcc aaacggtatt ttgtgttctc
    17581 agctttagag gcactttcca acaattctgg gaatttttga tacaactcgt taaagaaagt
    17641 ctcatcattt actaaatcta aaaattcttt cttgtcttta ccagttacct tcgctacccg
    17701 ctcagcaatc tgatcgatgt cgtagccttc tggaatggca attttcccat ctgcaacttt
    17761 tgttggctca gaagtgccgc cttcttgcag ctgtttgcca atctcgtcaa gagtcatatt
    17821 aggcgctaac tgataatagc cagcttgaaa gccagttaaa tttttaaatt ttgtataata
    17881 attgaaaaca atcccgcttt taataatatt gtccttctcc aaaatctcac caatctgctt
    17941 gttagaagag ccactcggga tctccacttg gactaattgg tcatctgatt tatctaaagg
    18001 cttcaacccg ctatctacat aacgatacac ggtaaatcca agaaatccgc caataattaa
    18061 taatgtaaga gcaatcacta aaatgatctt cctcacaatc ttatcttctt ttttacgcag
    18121 atttctccgc ttgtcgatta tatctgctgt agctgcctta ctggcttctt ttttatcttt
    18181 acttgggtgt tgtttgtttc ctgacaaaac aactcctcct tactatcatc ttcgtaactc
    18241 tgtttattat acatgaaatt tctactgccg tgaattattt taccaaatat tatgtcaaaa
    18301 ttgtaagtgg caaatactct ttttgaggat tacaatgaaa aatagttaaa aatggataga
    18361 aaggaattaa catgtcaaaa ggaaaacatc gaaaaaaaca aaaaatttct atttcttttc
    18421 ttgtaatttt attattgtct gtcagcttct tttcaacagg catcattata ggaggtcaat
    18481 taacaaaaat acctcattcc caaacttata aagcggagcc aacacaactt acttccaatt
    18541 taacgcccga tgagcagagg cttgctagca aaattgatca attactacaa aaatcagatt
    18601 atatcggttc gatttatgtt cgcaaaaata atcgcgtact attagaaaaa ggatatggat
    18661 atgcaaatct gcgcgtaaaa gaatcgaatt caccttctat ttactatcaa atcggttcga
    18721 ttcaaaaagc aatgactgct ttactgatac taaaacaaat ccaagctggt aagttaactt
    18781 ttgacacaaa gttagcagaa ttttatcctc agattcctgg aagtcagcaa atcacaatca
    18841 aaaatatgct ttatatgcgc tctggtcttc atcgaactgc ttctcccgac aaacctatgt
    18901 cggatagtga aatcattcag ttcgcccttc atcatttgaa acttactgat tatgatacgt
    18961 atcattatga accattgaat tatacattgc ttacagggat attgatcaaa ctaacgaatc
    19021 agccttatga aactcttcta aaaaaagaaa tcattcgtcc actccatcta gaacatacag
    19081 atttttatga caatgtaaaa aattcgcctc aacatgctgt atcttaccag atgtctgcaa
    19141 ctgacgatta ttcaaaagca ttgatcgagc ctgaaaccga catccgaaat gaattaggta
    19201 caggaaacat aagtatgtct gtttatgact tgaacaaatt ctttaccagt gttctttctg
    19261 gcggtatcat accaaaagaa ctacttttct cattgtggca aaataatggt gataaacatc
    19321 cttacagcgg cggtgtttac agtggaaacg attatatcct tgctcagggg aatatcaatc
    19381 gattccatgc agtagtggcg atgaaaaaag acactaaaga tgcaatcgtc atggaaagta
    19441 atatccaatc ggataagaac atcaaattgc cggccactga tttaagaaat cagatttatg
    19501 aactgatgga aggtgttcac ttatcaaatt aaattttgac ataatattag ttgtcatctc
    19561 tgtatagtca atttagttag tactctatct gaaaagtaga aataaattaa caaatattat
    19621 ctgtcctgaa taattcatac cgataaaaaa atgatctgtt gctctaaatt ttaccggaat
    19681 ttatattttt tattccgtct cagaacccaa cagattaaaa aatccgaact gtttaggtaa
    19741 acagctgcgg ttcagccaaa tttgggcgca ctcgtccctt gaagtacaat ataattttta
    19801 aaattgggat ttatttacca gcagagctgc tggatatttc cgaggacttg ataaaataag
    19861 ccctgatgta aatgataaga actgagtttc agcagcagtc tacgtcctgt tcgaacgagt
    19921 ttacctgcga ccttgaatag ttttaatcgg attgtgtcca cctgcatgct ggccgatccc
    19981 tgtgtgaaac aaagcgtacg cataaagtta acgatgttgt aagccaacag gcttaccatc
    20041 atgcgagcgt gattttctaa aaaggaaggg ctatcggtct tatcgaaata aaagccattc
    20101 ttcgcttctt taatgaagtt ttccatggtg ccgcgtttac agtaagtccg aaagactgtt
    20161 tccgcagaga cattattgga gtaattggtg acgatgtatt catgtcggaa tagaagttca
    20221 tcagcttccc gtgttgattt aatgcagact cggcgttctt tggaccaact ttttgcttga
    20281 taggatgttg aagaataaac aacttctttt ttatcccaag ggtggttatc gtcaatctga
    20341 ataaagcttt ccgcaatttt tgaaaggtta cggttcgcct ttaagcggat cacaaagaag
    20401 ctgtcataag cttcacagag atcgtaaagt tccggtgtag cgaacccgct atctccacgg
    20461 acgagaatat tgctgacagg aaccacttgg ttataatgct caaaaagagg ctccataaat
    20521 gtcccaacgc cattagacgt gtagacattg ccagaacgaa gttctgcttt taagaaatct
    20581 cccgtcaaac catcaaatgc gactaacgga tggtatccat tggttcgata atgagcattg
    20641 taatcagtgt tttcttgatc tccgtaagta tcagaatggg ttgaatccaa gtcaaagatc
    20701 atttctgtcg tatttcttgc cgaccgtact ttatcaatca tggcttggtt aagatcttgc
    20761 aactgagaaa tattttcttc gcttatgcga tcccagaatc gggaaatcga agactgcgag
    20821 gcaagcctgg acttatccaa aatcaccttg aatatagggt cttgcttcaa tatatttgcg
    20881 gaggaatcgg tcgaatagcc agcgatattt tgataaatca actgctccat caaagaaaaa
    20941 ttgtcatgga tatgatagag acgtttgtcc tctattccca agtattgctt agaaagatcg
    21001 gagaaattaa gagactccat aaattctttg acgagaacca atccagaatc ggtggacaaa
    21061 tttccacctg tgtttgaaat cgtcagttta gagttgaatt gtaccttttt ttcgtgtaat
    21121 gtagccattg agagagcccc tttctatggt tttagtgtgg taacttaacc atatcagatt
    21181 ggggttcttt ttgtacaccc aaagggtgct tatcgaacaa gtgaaaaatg ctattgcagc
    21241 aaggttattt gtagtttcct gaaattttat gaattattca ggtagaaaag ctgtaaaaag
    21301 tcttttaaaa ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt
    21361 gttcccggat ttcccagaaa aggtggtaga aagtggggga ttgtggtaga ctaattttag
    21421 ttagtggagg aatggggggc tttcaatgtt gatgggtgaa ttccaacata atattgatgc
    21481 aaaaggacgt ttaatcgtgc cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct
    21541 gacacgcgga ttggatggct gtcttttcgg ctatccgatg agcgaatggg aaaatctgga
    21601 agctaaactg aacgaaatgc cgttggctaa aaaagacgca cgtacttttg tgcgattctt
    21661 ctattcggct gccactgaat gcgaacttga taaacaagga cgaatcaata tacccagcac
    21721 gttaagaaat tatgcagctt tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat
    21781 tgaaatttgg gatgagacac gatggcaaga tttttctgca gcagcagaag agaactttga
    21841 cgaaattgct gaaagtatga ttgatttcgg actataagaa taggaagagg ctgactaatg
    21901 actgaaacat ttcaacacta tacagtcatg ttgaaagaaa cggtagatgg attgaacatc
    21961 aaaccggatg gcgtttatgt agactgtaca ctcggcggtg ccggacatag tgaatattta
    22021 ttaagccaac taagttctaa agggcatctg tatgcgtttg accaagatca aaaagcaatc
    22081 gatcacgcca agctgcgttt agcttcttat gtcgaacaag gtatggtcac atttatcaaa
    22141 gcgaacttca gagaattaga agaacgcttg agtgagcatg tagataaggt agacggcatt
    22201 ttgtatgatc taggggtttc ctctccccag ctagatgaag cagaacgtgg atttagttat
    22261 catcaagatg caccgttgga tatgcgcatg gatcagtcag ctcctttatc cgcttatcac
    22321 gtaattaatg aatattccta ccatgaatta gtaaagattt tcttccgcta tggcgaggag
    22381 aaattttcca aacaaattgc tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa
    22441 acaactggtg agcttgtcga aatcattaaa caagtcatcc cagctcccgc aagaagaaaa
    22501 ggcgggcatc ctgcaaaaag aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc
    22561 ggtgccgaag aggcctcttt agaacaggca atccggttat tgaaagtcaa tggaagaatc
    22621 agcgtaatca cattccattc attggaagac agaattgtca aaagtatgtt taaagaatat
    22681 agtacggtac aagatctacc accgggactt cctgtcatac ctgacgaatt ccaaccagaa
    22741 ctgaaagtca ttactcgtaa accaatcctt ccttcagagg aagagctaac agaaaacaat
    22801 cgatcacgca gtgcaaagtt acgtattgca gaaaaaataa gaatgaaaga ggagtagaat
    22861 gatggcagaa ttaaagaaaa tgcaagatta cgaatatgat ttacctgaat tggtggatca
    22921 gccggaacaa caaatgcctg attcttcttc gaatcaagaa atcattcttc cacaatcacc
    22981 ggcaaaacga ttaaagcaga tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat
    23041 tgctattgtt gctttatgtg ttttaacagt tatgctgcgc acaaatatca gcggagctga
    23101 aaaagaaatc acgtcaatac aaacagaaat caatgataaa aagcaagaaa aaacgagttt
    23161 agaacaagaa aaaaacgaac tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa
    23221 aggattgtct attaatgacg acaatttaag gaaagtgaag taaatgagtc ttaaaaataa
    23281 aattcggcgg tttatggaaa agaaaaatct aaatccgatg aacaatcgca aaaaagtagg
    23341 tatcatttta tttgctacga gtattggatt gttcttttta tttgccgtcc gtttctcgta
    23401 tatcgttatt ggaggtcatg ttgctggtac ctcattagct gaaaaaacaa aacagttgta
    23461 ccaaggaagt gaagtagtaa aagcaaaaag aggaacgatt tatgatcgta acggtgtggc
    23521 gctcgcagaa gatgcctcct cttattcaat taaagcgata ctctcaaaaa catatacctc
    23581 tggggataaa aaactgtatg ttgaagaaaa gaattttgat aaaatagcag aaatcctgca
    23641 taagaatctt tctatagaca aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa
    23701 agagctgtat caagttgaat ttggtagcta tgggaaaaat atctcccaag aaaccaagca
    23761 aaacatcgaa gcggacatga aaaaagaagg tgtcgctggt ctttattttg tcgaccatca
    23821 ggctcggatg tatccaaatg gcgtcttttc ttctcatttt attggatacg cagttccaga
    23881 caaagatgag aatggcttag ttggtaaact aggtttggag tctgcttaca atgatatctt
    23941 gagcggtaaa gatggtaaga ttatttatca aaaagataat tttcaaaatc ctctgccagg
    24001 tacagtggca gaagaagaaa aagctgtcga cggacaagat atctacacga ctttagacag
    24061 cagactacaa agttatttag aaacactgat ggatcaagta aatgaagaat atcagccaga
    24121 agaattgaca gcagtcctga tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg
    24181 acctactttc aatcctgaaa caatggaagg attgactgga aaagatgcta tctggcgtaa
    24241 tttcttggta caagatagtt atgaaccagg atcaaccatg aaagttttca ctactgcagc
    24301 tgctatcgaa gaaggagaat tcaacgagaa tgaaaccttc caatctggaa aaatccaagt
    24361 agaagatgct acgatcaatg accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca
    24421 agctctttca tggtcaagta acgtcgggat ggtcatactg gaacagcgat taggcggacg
    24481 atggtataac tatttacaaa aactgggatt tggacaaagt actcattctg gattagatga
    24541 tgaagtaaac ggtgctttac ctacattaaa tattgtcgac cgagcaatga gtgcttatgg
    24601 acaagcggta ggtgtgacga atttccaaat gatgaaagct ttcacttcta ttgcgaataa
    24661 cggtacaatg attcaacctc gatatatcag caaagtggtg gatccccaaa ctggagaaga
    24721 gcgaacaact caaacagaag ttctagggca acctttttca aaagaaacaa cagaaaaagt
    24781 ccgtgaatat atgagagatg ttgtcgaaag cgaaaactac ggtattgctt atggtgtata
    24841 tagtgtacct ggatacaatg tatcggcaaa aaccggaaca gcccaaatcg cttctgatac
    24901 agggggatat caaacaggag atacagccta cctgtattcg attgtggaga tggttccttc
    24961 tgaagatcca gactatgtct tgtatctaac aatgaaacac cctaagacat atgatcgaat
    25021 ggcattggct aaaatcgcaa atccattgat gaaacgggca atggatttta aagaaactga
    25081 agaagattca gatacagaaa caaaaacaga aaaagtatcc gtagccgatt atcgtaatct
    25141 tgaagcggac gttgcagcag ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg
    25201 taatggtaaa aaagtacaaa aacaatctac tgccaacggt gatcagttga tctctggaga
    25261 aaaactgatc ctatatactg gtggtgataa gctgatgcct gacgtaacag gatggtcaaa
    25321 agcagatatc atgaagctgg ggaaaatcct cggtattgaa gttagttttg atggagatgg
    25381 ttattgtgtc aaacaagaac ttgctccata tgaaaaaatc actgaagaca aacttagttt
    25441 tactttagaa gaataagaag agaaatagga gagaaaagag catggaatgg acacaagcat
    25501 tgattcccat tgtcagcagt tgtgcaatga caattgccgc aatgccgcta tttattggtt
    25561 attttcaaat gaaaaagcaa ggtcaggcga ttcgtgagga gggaccaaag tggcacaata
    25621 gcaaagccgg aactcctaca atgggcggct tagtgtttct gatcggctct attttgacag
    25681 gaatttgggt tggagcttgg caaaaacagc taactcctac tcttttcatc ttgctttttg
    25741 tattagcgtt atacggagta atcggctttc ttgatgattt tataaaaata ttcaagaaaa
    25801 gaaacatggg actgaactcg aagcagaaat tgattggaca gattctaggg ggaatcatct
    25861 tttatcttgt ttaccgttct gaaggttatc caggtgtatt gaatttcttc ggactagatc
    25921 tacctttagg aatcatttat ggtattttcg ctctcttctg gcttgttgga ttttcaaatg
    25981 cagtaaatct gacagatggc atcgatggat tagttgctgg cttaggaagt atctcttttg
    26041 cagcttacgg tctaattgct tggcatcagc atcagtatga tgtattaatc atttgcttaa
    26101 gtgttttagg aggtttactg ggtttcttcg tctacaacag aaaacctgcc aagattttta
    26161 tgggagatgt cggttcatta gcgttgggcg gacttttagc ggcaatttct ataatgctaa
    26221 accaagaatg gacattgctt ttgattggtt tgatctatgt tatggaaaca gcaagtgtga
    26281 tgcttcaagt tacttccttt aagcttacag gaaaacgaat ttttaaaatg tctccaattc
    26341 atcatcactt tgaaatgtgc ggatggtcgg agtggaagat tgatacagtt ttttggttga
    26401 tcagcattgt gacttcgttg atcacattat ggatcgtttg gtaggagatg acgggaaatg
    26461 aaaaaaatat caacatatga aaataagaaa gtgcttgtct taggactcgc aaaaagcgga
    26521 gtaagtgcgg ccaaattatt acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa
    26581 ccttttgacg aaaatccgga agcacaagag ctgctttctt tagggataaa agtaataaca
    26641 ggtagtcatc caatcgaatt gttggatgaa gaattttcat tgatggtcaa aaatccaggg
    26701 attccctaca gtcatccttt agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg
    26761 gaagttgagc tagcttacga agtagcggaa tgcccaatta ttgggattac aggaacgaac
    26821 ggaaaaacaa cgacaacaac gatgacagga ctactactga atgcaggagc agatcaagga
    26881 attgcacgtt tagcgggtaa tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa
    26941 tcagaagata aaattgtgat ggaattgtct agcttccaac tcatgggaat cacagatttc
    27001 cgccctcata tagcggtgat taccaatatc tatgaagcac atattgatta tcatggtaca
    27061 agaaaagaat atgtcaaagc taaatggaac cttcaaaaaa acatgacaga aaaagattat
    27121 ctgatcctta actggaatca gtcagaacta caagaattag cgcaaagaac aaaagcacgc
    27181 gtactgcctt tctcgacaaa agaagtgttg gaagacggtg tgtatgctga cgattactca
    27241 atatactata aaaaagaaaa aataatggaa atatctgaac ttggcgtacc aggtaagcat
    27301 aatgtagaaa atgcactagc tgctatttct gtagcaaaat tgtatggtat atcaaatgaa
    27361 gctatacgag aaacgttgca ttttttccat ggcgtaccac atcgtacgca atatgtagga
    27421 gaaatccaag gcagaaaatt ttataatgat tcaaaagcaa ccaatatttt agcgactaaa
    27481 atggctctaa gtggatttga aacgtctaag gtcgtgttat tggctggcgg attagacaga
    27541 ggaaatacat ttgatgaatt aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt
    27601 ggccaaacaa aagaaaagtt gatggatgct ggaaaaaaag caggcatcga gacaatagta
    27661 acagccgatt ctgtagaaca agcagttcct ttagcattag aaaacagtac agatggtgat
    27721 gtagttttat tatcaccagc gaatgccagc tgggatcaat acccaaattt tgaaactaga
    27781 ggaaacagat tcatggaagc tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata
    27841 aaaaatgaaa attttggtta caggcggagg aacaggggga catatttatc cagctcttgc
    27901 ctttgtcaat tatgtaaaga ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa
    27961 acgaggtcta gaaaacaaaa tcgtccctga tacaggaatg cctttccata ccttggagat
    28021 tcaaggattt aaacgaaaaa tttccatgca taacatcaag acggtccgat tgtttcttaa
    28081 gagtatccgt caagcaaaaa aaatcctcag tgattttcaa ccagatatcg ttatcggtac
    28141 tggaggatat gtttccggag cggtagtgta cgctgcagcc aaaatgggta ttccaacagt
    28201 tatccatgaa caaaacagtg taccaggtgt taccaataaa tttttaagcc gatatgtgga
    28261 tcggatcgct ctttcattta aagatgcggc ttcctttttt cctgaaaaca aagcagttct
    28321 tgtaggtaat cctcgagcac aagaagtagc agatactaaa aaatcagata ttttgaaaac
    28381 atttgatttg gatccagaaa agaaaacagt cttgattttt ggcggtagtc aaggagcact
    28441 taaaatcaat caagcagtat cagttttttt aagtgaatat attccttctt cttaccaagt
    28501 tttgtatgct tcaggagata gatattatga agaga
//
LOCUS       DAXWLH010000016        28321 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXWLH010000016 DAXWLH010000000
VERSION     DAXWLH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.016"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            242..2077
                     /gene="tdc"
                     /locus_tag="ACGE36_000512"
     CDS             242..2077
                     /gene="tdc"
                     /locus_tag="ACGE36_000512"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8382935.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2382..3860
                     /locus_tag="ACGE36_000513"
     CDS             2382..3860
                     /locus_tag="ACGE36_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8382936.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3826..5157
                     /locus_tag="ACGE36_000514"
     CDS             3826..5157
                     /locus_tag="ACGE36_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8382937.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5271..7823
                     /locus_tag="ACGE36_000515"
     CDS             5271..7823
                     /locus_tag="ACGE36_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFF8382938.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7830..9269)
                     /locus_tag="ACGE36_000516"
     CDS             complement(7830..9269)
                     /locus_tag="ACGE36_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8382939.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9244..9426
                     /locus_tag="ACGE36_000517"
     CDS             9244..9426
                     /locus_tag="ACGE36_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382940.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9615..9977
                     /locus_tag="ACGE36_000518"
     CDS             9615..9977
                     /locus_tag="ACGE36_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFF8382941.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10164..11021)
                     /locus_tag="ACGE36_000519"
     CDS             complement(10164..11021)
                     /locus_tag="ACGE36_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8382942.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11141..12052
                     /locus_tag="ACGE36_000520"
     CDS             11141..12052
                     /locus_tag="ACGE36_000520"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8382943.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12059..13705
                     /gene="mdcA"
                     /locus_tag="ACGE36_000521"
     CDS             12059..13705
                     /gene="mdcA"
                     /locus_tag="ACGE36_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFF8382944.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13807..14106
                     /locus_tag="ACGE36_000522"
     CDS             13807..14106
                     /locus_tag="ACGE36_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFF8382945.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14096..15772
                     /gene="mdcD"
                     /locus_tag="ACGE36_000523"
     CDS             14096..15772
                     /gene="mdcD"
                     /locus_tag="ACGE36_000523"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFF8382946.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15776..16369
                     /locus_tag="ACGE36_000524"
     CDS             15776..16369
                     /locus_tag="ACGE36_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFF8382947.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16393..17469
                     /locus_tag="ACGE36_000525"
     CDS             16393..17469
                     /locus_tag="ACGE36_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8382948.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17494..18363
                     /gene="mdcB"
                     /locus_tag="ACGE36_000526"
     CDS             17494..18363
                     /gene="mdcB"
                     /locus_tag="ACGE36_000526"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFF8382949.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18474..19238
                     /locus_tag="ACGE36_000527"
     CDS             18474..19238
                     /locus_tag="ACGE36_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFF8382950.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19727..21820
                     /locus_tag="ACGE36_000528"
     CDS             19727..21820
                     /locus_tag="ACGE36_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8382951.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21845..22147
                     /locus_tag="ACGE36_000529"
     CDS             21845..22147
                     /locus_tag="ACGE36_000529"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8382952.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22174..23463
                     /locus_tag="ACGE36_000530"
     CDS             22174..23463
                     /locus_tag="ACGE36_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8382953.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23482..23889
                     /locus_tag="ACGE36_000531"
     CDS             23482..23889
                     /locus_tag="ACGE36_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8382954.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23912..24781
                     /locus_tag="ACGE36_000532"
     CDS             23912..24781
                     /locus_tag="ACGE36_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8382955.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24783..25403
                     /locus_tag="ACGE36_000533"
     CDS             24783..25403
                     /locus_tag="ACGE36_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8382956.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25853..26458
                     /locus_tag="ACGE36_000534"
     CDS             25853..26458
                     /locus_tag="ACGE36_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8382957.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            26593..27045
                     /locus_tag="ACGE36_000535"
     CDS             26593..27045
                     /locus_tag="ACGE36_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8382958.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            27082..27579
                     /locus_tag="ACGE36_000536"
     CDS             27082..27579
                     /locus_tag="ACGE36_000536"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8382959.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            27597..28307
                     /locus_tag="ACGE36_000537"
     CDS             27597..28307
                     /locus_tag="ACGE36_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFF8382960.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
BASE COUNT         9305 a         4841 c         6028 g         8147 t
ORIGIN      
        1 tttgtttgtt cgcttttttt aggttatcta ttttagaaga ctgatttatt gaaaaattgt
       61 tcgctttttt gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa
      121 tctacgtgga tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg
      181 tgttacagat aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa
      241 aatgaaagat atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt
      301 ttataaaatg ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat
      361 tccttcagat atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac
      421 ccgcgaccat atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat
      481 cccatggcat tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc
      541 tttattagct tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc
      601 tatggcaact tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat
      661 gacagacgga tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg
      721 gtacgcccgt tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt
      781 gaaaaaaatg tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac
      841 cgaaagcttt acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa
      901 aaatatccaa agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa
      961 ggcgttagat atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga
     1021 ttatcgaatg gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat
     1081 cccaattcta ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt
     1141 agataaaatc gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca
     1201 tgttgatgcc gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt
     1261 tgtgccgtat gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt
     1321 aacgattgac aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac
     1381 gatagatcct cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca
     1441 taaaaatatg agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa
     1501 ggctccagat atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc
     1561 cgcagtctgg acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat
     1621 tggggcttca atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac
     1681 tgttaaagga aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa
     1741 ttgggtattc aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa
     1801 aatgttcgat cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc
     1861 tcatactaca tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat
     1921 gggtctaacg aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat
     1981 catgactcct tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa
     2041 agcaatggaa aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat
     2101 aacaagagac atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc
     2161 ctgtttttca tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga
     2221 tcgttcgttt catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat
     2281 actgggtttt gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa
     2341 gaaaacaaaa acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat
     2401 caaagcaaat cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac
     2461 gaaatattcc agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag
     2521 tattattgta cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc
     2581 aaaaaggtgg tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg
     2641 tctcatggct gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg
     2701 ctccgttgtt tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat
     2761 tagttgtcat tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg
     2821 caaaaatcgg cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc
     2881 tcttattagg gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca
     2941 gtttttcatg ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg
     3001 cgccgatcat gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg
     3061 aaaatccagt taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg
     3121 tcaatatttt gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga
     3181 ataatatcgc gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg
     3241 tgaatctgtt tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct
     3301 caggaccagc aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca
     3361 atttttggaa gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca
     3421 ttatctcaat ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga
     3481 tgctcgtaaa ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag
     3541 ttttgagatt gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag
     3601 agaaaaagag caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat
     3661 tttctgtcgg actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca
     3721 tcagtgtcat tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg
     3781 aacaggaagt cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca
     3841 atgaaacgga ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga
     3901 acaggattat ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc
     3961 gtttatttga tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt
     4021 ttagcaaata gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag
     4081 tgggcatttt ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa
     4141 ttaacagcaa taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc
     4201 ggaggtagcg cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt
     4261 gaattggaat cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg
     4321 ggtataggat tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt
     4381 ggacagatcg atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg
     4441 gttttattca gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat
     4501 ccgcaaaaga atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat
     4561 ataggaacga ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa
     4621 agtcctttag tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat
     4681 ctaatgattt tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg
     4741 attttatatc aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag
     4801 aaaggcgtac ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt
     4861 ctacattttt ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg
     4921 agttgttttc tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa
     4981 aagaaacgga acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca
     5041 attgctttca gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc
     5101 tggtttgtat tcttaaattt tgtatataaa agaataagca atagaaatat cagttaggcc
     5161 cagatacatt ttgcaaaacg aattatttca gacccccctg aaataatcga tacgcctgaa
     5221 aaaacttccc tgcgtataat aacagtatca attaaacagg aggggatagg atgaactggt
     5281 acagattgac ggtagaagat gtattgaaaa actatcaaac atcaacagaa ggcttgtcgc
     5341 aagaagaacg acagaaacgc ctgactgatc aaggatataa tgaaataaaa gcaaggcagc
     5401 agacaaaacg atggaaaaaa atagcaaagc attttactga tctgctgatg atcgtgttga
     5461 tcattgcctc ttttttgaaa tttgcttcta gtgaatatat tgaaggaagt attattttat
     5521 tcgtagtgat cgtcaatgga ctcgttagtt actggcaaga aagaaaagcg gaagaatcat
     5581 tgaatggttt gaagcagctg atgggacaag aagcgatagt actatcaaac gggatgcaag
     5641 aaaaaatccc tgcgaaagaa ctggtaaaag gagatgtcat tgtcttacgt gccggagatg
     5701 tcgttccagc agatatccga ttaatcgaag cacatgattt attagtggaa gaatcgattt
     5761 tgacgggaga atcagaagca tctgaaaaaa gtcatgccgt tttgacagaa gaagaatcga
     5821 tcggcgacca gaaaaatatg ggtttttcag gaacgcttgt acaatctggt tctgcagtcg
     5881 gtgtcgttgt agaaacagga aacacgacag aaatcggtaa aatcaatcaa gcattgcaat
     5941 cggtcgaaca acaaacgacg cctctgatta aaaaaatcaa ccgattaaac aaacaaattt
     6001 ttcaaggcat tctttgtttg agcctattct tagttatttt tacgacattt cgatatggta
     6061 tggattggca tattcttctt tcggctacga ttgctttggt ggtgtcaatg gtacccgaag
     6121 gattgccagc tgttttgacc atgattctat ccgttggagt acatgaaatg gcaaaagaaa
     6181 aggcgatcat caaaggactt ccttcggtag aaacattagg ttctatgaca gtgatctgtt
     6241 cagataagac aggaacattg acaaaaaatg aaatgacagt tgtggatgta gctgcaaaag
     6301 aagaagcctg cgtgttgtcc attatgaaaa actgtcagga attaaaaaca aaagaagaac
     6361 agaaaacaga aaatttaagt ggcaatccga cagaagttgc gctgattcga cacacagaaa
     6421 cagccaatct acctttgaaa aaaatagaag caaagattcc gttcagttct gaatacaaat
     6481 atatggcaac gatgcatgcg gaggaaaaag gagcggtgat ttacgtcaaa ggtgcaccag
     6541 aagtattatt tgctaaatca actctttctt ctgcagaaca agaagcatgg agtcagacag
     6601 ctgcagaatt tgcgagaaaa ggtcagcgtg ttttaggatt tgcttataaa aaagtagatt
     6661 cgaaacagga actcacgcat gagactttaa cccaattaac ttttgcaggg atcgccggat
     6721 tgatcgatcc gccaaaagaa agtgcagtca aagctgtaaa ggaatgccaa caagcaggga
     6781 tctcagtgaa aatgattaca ggggatcaca aagacacagc aaaagcgatt gcggaacaaa
     6841 ttggcttaaa acatactgcc aaagttttag aagggatcga tttggatctt atgtctgatg
     6901 aagagttgat tcagcaagtg ccaatagtcg atgtctttgc tcgaacgaca ccagaacata
     6961 aactgcgaat tgttaaagct ttgcagaaaa atggagaaat cgtcggaatg accggagacg
     7021 gcgtaaatga tgcacctgct ttgaaaagat ccgatgtagg aattgctatg ggaatcaaag
     7081 gtagtgaagt aagcaaacag gcagctgata tggttttagg agatgataat ttccatacga
     7141 ttgcaaaagc cgtaaaagag ggccggcgga ttttggataa tttgcaaaaa accatcaatt
     7201 tcttcttgcc aactgcatta gctcaaggat tgatcttgat ttgggcactt atgctgaatc
     7261 gcccgctacc tttgtcccca gtacagattt tatgggtcaa tatggtaacg acaatcactt
     7321 tatcttatgc actagggttc gagtcgccag atccagaaat catgaagcgc ccaccaagag
     7381 atccaaagca aggaatccta tctggttatc atttgtttcg gatcatctat gtctccctac
     7441 tgatcatggt tcctgcctat ttattggcta tgcagtttga agggcaaagc ttacagcaaa
     7501 ccattcttct gcaaaatatc gtcttagctc aagctgttta catgatcaat tgcagagagt
     7561 tgtccaagcc gtcattgaac caaggtcttt tgcaaaacaa agcgcttttt ctttcattag
     7621 gtatccttgc tttattgcaa gggatggtta tttttctacc agtcggccag cagctgatcg
     7681 gtacagtaag tttaacgatg acacaacagt tattgattgg agcaaatgtg attcttctat
     7741 tcttagttgt agaaatagaa aaagggatca tgaacaaact gtttcgcaaa aaagaaaaaa
     7801 cagctagaga atacagtcgc tagccgttac tataaaaaag ttttgaaagt gagttcagtc
     7861 aaacgattga gctcattttt cgttggaata gaagaaatgt atacaatagg gatatctaga
     7921 gaatctagag ggaaattgga aatcaagata tctgtttttt caaaagaaat agtggaaaga
     7981 tcggttagtt cgataggtaa gaagcagaca cgttttccta agtaatcttt taattcctgc
     8041 tgcaagaacg ccttccatga aggttctcct tggaaaataa aatagacatt gaacaaattc
     8101 ggtttgatag aaaaaatcgt ttgttggatc agtagagaca agagttggag aaagtaattt
     8161 ggttcattga tttctatttc tttttttaga tcgttcagta aattctcttt ctgttgatac
     8221 aagtgtgcat attgctgatc atatgtttct aaaaaacgat ggtattgacg gtccaatgct
     8281 tcaatcaata tcggcgattc atgatatttt aatatgagga gagccaagtt atcagtcaga
     8341 gatgtttttt ttagttgatc aagagacagc tctgaagcta gttcctctac aaacgtttcg
     8401 aggacctcta aaaatggaga atgatgcagg cagcgagcgg tttcttgtcc atagttattg
     8461 ttgtaattcc aactttccag aaagcagaaa gaagcaaaag aaagctcagt gtctttaaga
     8521 taaatacctt ccgttaaata caattttttc aatgcagagt catacaggga ggagagtgtg
     8581 ctgttggtat ggatagaaga atctaatcgg caattcgctt ttatacggat cgtattgata
     8641 aagaaccaac atgttccgaa aatctcttct gtttcaaccg ctagttcagt ttgactgagg
     8701 tttcttaaaa attgaaaata atgggattca tggatcgaat aatcctcgaa aaaatagttg
     8761 ttatgagtga aaggaatcag caatcgatga taaaaagtac gaatcgcacg ctcttttcca
     8821 agaatacaag ctgtatattt cgtcaaatcg atctgcaagt cgtaacttgc taaacgctga
     8881 ttgattttag tcacatgtct tttcaatgtc ccataagaaa gcccatggtc ggctagaaaa
     8941 tcagggacag caactttttt tgtaaaaagc agatttttca gcagttcgac actgactgat
     9001 tgtttgaaaa aaatctccca aaggtcattt gttaataaat tttgctggtt ttgcaaactg
     9061 attcctgctt gatcgctgat gatgtcccag ccttcgggca gctgagagcg aatagattga
     9121 aggtcagaaa aaatcgttcg ttcggttgtc cctatgattt cagctaattc atttacggtt
     9181 atctttgtat gattcagcaa ttgttctact aaagaaacat gacgtcgaat atctttttcg
     9241 gtaatgattt ttttcattag tgaatacatc aatcagactc ccttctattc taagaatacg
     9301 gagaaaataa acggtggtca agtttcaact gatttttcaa tcattgttta tttttctgga
     9361 gcatttactg atgataagaa tttcatcatg aacttgaaaa atgataccac taaaatcaaa
     9421 aaataggtgg gcaaacaata ataaacattt taatagtaga tggtagcaag accaacacag
     9481 gcggattgaa catttaagag ttgataaaaa agaaaaatat cgaagtattt gattagcaac
     9541 tacttgtatt catttttcta ttcttacgtg gtatctttga ataaagcgaa tcaataagaa
     9601 agagatggaa gcagatgcta acgaaaaaag aactaatcga tcacatcaat gaaacataca
     9661 atttgtctcc agaatatatt tttaaaaaat ttcctgatta ttgcgtattc agacatcaaa
     9721 aaagcaaaaa atggtttggg gtcctgatgt ctgttccaga agaaaaaatc tatgatgatg
     9781 caagtgacca taggatcatc aatattcttg atttgaaggt cgatcctgaa cttggcggaa
     9841 ttttgcgagg aaaagagggg tattttccag cctatcacat gaataaagaa cattggatct
     9901 ctattgatct acgtaaaata gaaaagctag atgaactagc agatttgatt gatggaagtt
     9961 ttaaatcaac gcaatgaatg tctaggacaa tagagaagag gttgtgacag aaatgtttag
    10021 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttggg aaatttcggc
    10081 ttattttcta agaaattact tctggaacac cgtttgttcg aaaaaagagc tgcgacaaga
    10141 attttgtccc agctcttttt tttttatttt cctaaataaa attcccgcat cgtttttgca
    10201 atcattttga tcaaaggatg atcttctgtg aagctagaag ataaataaat tgttcgtgtc
    10261 aagggcaaat ctttgatttc ccgcaagacg aaaggatcat ttttatcgta agaccaagaa
    10321 acttttggga aaaagctaac agctaatcct tgcttgacaa tagaacgtaa tgtcgcagga
    10381 tcatctgtcg tcgaactgat cgtcaattcg atacttttcg cttcaagtgc tgcatccagt
    10441 gtatgacgga aggcgttgct ctttgacagc atcaagatgg gggtgcgttg gatatcttct
    10501 aattgcagat gttctttttt agcaagagga tgtttcttag gaatggcgac ataaacaggt
    10561 tctttcatga taggaatatt ggtcatgcca ggcttcatct ccgaagtgat gtacagtaca
    10621 ggatcggtaa tatcatctgg taaatgatgt tgcatgatcg ttaattggat atcaggatgc
    10681 ttttccataa aaattcggac caagttcgga atagaaacag aggctacctc taaatacaat
    10741 atgacggaag taacgatctt ctcattgacc gaagacaata atttgattcc ttcttctaat
    10801 gtgtctaatg tcttgactgc atatggataa aattcttctg cataaacggt cgtctcaatg
    10861 tttctgccgc tatggtaaaa caaagggaca cctaattcgt tttccaaatg tttgatcgca
    10921 cggctcaagg caggttgaga aataaataat tctttggctg cttgggtaac attttttgtt
    10981 tcgaatactt ttttaaaata ctgcagatga agcaatttca taattatcca tcccatccat
    11041 acctttttgt tatggttttt tctttttttg gcatttttta ttatagcaga aattgactaa
    11101 gatagcagtg gaaaaagtaa gtgctgagga ggcgaggctc atgagtacag gtttcttatt
    11161 tcctggacaa ggcagtcaga agttagggat gttagcggat atccctgaaa tgtacatgga
    11221 acgtttagaa aatgtgacgg ggtatcggtt agttgataaa gaagaaaact atcaagatac
    11281 ggtttttatc cagctagcat tactgacaaa agcagtattt tatttagatg aaatggaaaa
    11341 aaacggtatt cagcctgata ttgcagcagg acattcgatt ggtgcttttt gtgcagctgt
    11401 tgcttgtcga tctttgagat ttgagaatgc agcggaacta gtctatcacc gcgcagtctt
    11461 aatgaaagaa gcttatccaa gcggttatgc aatgggcgta gtagtaggtg tgacgagaag
    11521 cgaagctgag gaaattgtag caaaaacatt tgatcctgac catccagttt atctgtcaaa
    11581 tgaaaattgt ccgatgcaac atacgttatc tgggagtatc gaaggtttat ccgttacgtt
    11641 ggcagaagcc aaacggcacc aagcgcaaac agcgaagctg ctgaaagtcc ctgtgccatc
    11701 tcactgtctc ttgatgaatg atacggtcaa gaaattcaag ccgtatatgc agtctgtcca
    11761 tctatctgat gctagatgtt tgtatataaa aaatacggat ggaagagcca ctcaggacgc
    11821 agaagagatt cgtaaagatt tgttggaaaa cctagctttc cctgtgcagt ggaaccaaat
    11881 gatggacttg gcaaaagaat taggaatgga tctgacgatt gaatttcccc ccggcaatac
    11941 tttgacaaag ctgattcata caaaatttgg tgtagataaa gggatccgta cgatcaatct
    12001 agaccaacat ggtattgatg acgccacgtt tctatatcaa aaatggagat gactaattat
    12061 ggaaaaacca aaccgcaact gggcttctaa acgcgaaaaa aaagcgcagc gattggaaaa
    12121 aatcagcaac ttgctcaatg gcaagtatgt agaaactgaa aaaatcgtag aagtaatgga
    12181 aaaactgatt gctcccggcg acaaagttgt attagaaggc gacaatcaaa aacaagcaag
    12241 ttttctttca gaaagtcttg aaaaagtaga tgcagaaaaa gtgaatgatc tgcatttgat
    12301 catgtctagt atctcacgac cagaacattt gaatattttt gaaaaaggaa tcgcaagcaa
    12361 gatcgatttc tcttatgctg gtgcacaaag cttgcgtgtc gcccaaatga ttgaagacgg
    12421 cacaatgaaa ttaggtgata tccatactta tttggaactt tatggacgct tattcatcga
    12481 tttgattcca aatgttgttt tagtagctgc agataaagca gataaagacg gcaacttgta
    12541 cacaggtttc aatacggaag aaacaccaac aatcatcgaa gctgcagctt tcaaagatgg
    12601 tatcgtgatc gttcaagtta acgaagttgt cgattcttta ccacgtgtag atatccctgg
    12661 tgactgggta gacgttgtcg tgaaagcaga taaaccttat caattagaag ctttattcac
    12721 acgagatcct caaaacatca cagaattgca aattctaatg gcaatgatgg caatcaaagg
    12781 aatctacgca gaacacggcg tacaatcttt gaatcacggt attggattca atacagcagc
    12841 tatcgaattg ttactgccaa cttatggtga atcactaggg ttaaaaggga aaatcgcgaa
    12901 gaactgggta ttgaacccac atccaacgat gattccagcc atcgaatcag gttgggttga
    12961 aagcatccat agttttggtg gcgaagttgg tatggaaaaa tatattgctg ctcgcccaga
    13021 tgtctttttt gtcggtaaag acggcacgat gcgcagtaac cgaacattat cacaagctgc
    13081 aggacaatat gccatcgata tgtttatcgg ttcaaccttg caattagatt acgaaggaaa
    13141 ctcttctacc gtaacaaaag gacgtttatc tggttttggc ggtgcgccta acatgggaca
    13201 taatcctggc ggtcgtcgtc attctacacc agcatggcaa tcacttcgcg acaatgatga
    13261 tccattaggg aaaggccaaa aattagttgt tcaaatggtt gaaacatatg gttcaaataa
    13321 aaaaccagtc tttgtcgaag aattagatgc actagctgtt caaaaacaag caggcttagc
    13381 caatgcacca gtgatgattt atagcgaaga tactacacat atcgtgacag aagaaggaat
    13441 tgcttatttg tacaaagcaa aaacaaaaga agaacgacaa gcagcaattg cagcaattgg
    13501 cggtgttaca cctcttggaa tgactagcac gaaagaatca ctagatagct tgcgtgcagc
    13561 cggcatcgtg aaattaccag aagatctagg catcaaacgt agtgaagcga aacgttcatt
    13621 attagctgca caaaccatcg atgatttgat cgaatggtca gatggcctgt atgaaccacc
    13681 aatgaaattc agaacttggt cataaaattg gcgagaaaaa ccttttgtcg ccatttaccc
    13741 agatggaaga agaaagacga ataagcagaa cgtgtatgct ctgttttaca aaaaggggag
    13801 agatccatgg aacaattagc ttataaatat ccaacgaaac agccgatcaa taagaaaatc
    13861 catgtaggag tggtcggttc aggagatttg gaaatcttga tgtttccaac agaaaaaaca
    13921 gaatctactg taaaaatttg tacaggaagc gatggcttcg gcgaagtatg gaacaatgta
    13981 ttaacgagat tttttgatcg ttatccaatt tcagcagata tcgtgatcaa tgacttcggt
    14041 gctacaccag gagttgtgta cctacgattg acgcaagcaa tggaggaact ttcagatgaa
    14101 aaatagtttt gtagaattaa acgccagaga acgagtgaac gctctacttg acaatggacg
    14161 tgaattgatt gatccattcg atcaaatcat cgcgccaagt ttaccagcac aaggaatcgt
    14221 ccctgaaagt gatgacggga tcgttgtcag ccgtggcacg atcaatggca aacaagcagt
    14281 agtaatcgct atggaaggaa aattccaagg tggcggtatc ggtgaagttt ccggtgcaaa
    14341 aatcgttgct gcacttgaac atgtgttaga agaaaataaa aaaggaaatg agatctttcc
    14401 agtcattgta ctagatacag gtggtgttcg tttgcaagaa gcaaactacg gactgttatc
    14461 tatttcagaa atcggaaatg taatcattgc tctaaaacaa tatgtaccag tcatcggttt
    14521 gattccagga cgtgtaggat catttggcgg tatgtcgatt acttcagcat taatgagtta
    14581 tttaatcgca acaaaaaaag cgagagtcgg tttgaatgga ccagaagtta ttgaacaaga
    14641 agcaggcgtt cgagaatttg attctagtga caaagacttg atttggaata cattaggatc
    14701 tcgtcaacgg gtagaatcaa aaatcattga tgaattagtg actgatagtg tagaagcaat
    14761 caaagctgct gtcatcgcag caatggacga acgaaaagac acgcatcgca gtgaaagctc
    14821 agcattttat ttatcattac ttgattcttt agatctttca aaaccaatgg ccattgacga
    14881 ttacaatgaa gcaattgcta accatcaagc aaaatcaatt gacttaccag aagtagcaga
    14941 aggaaatgaa ccagctgcag caagtgttgg ttacgattgg tttgcggcac taactggcat
    15001 gaaaaatcca acttcttcta ttagtactgt gtattatggt aaatcaagta aattcataga
    15061 agaagccatc gtgacatcaa tcgtacctga tcctaaaaac ccgatgtacc gtgtacgaaa
    15121 cggtgaagtt ggtttagttg aaggtttccg catgggacaa attttggatc acgtttacga
    15181 agaagataaa gacaaaaaag tgaaacggcc aatcatcgtt gtgatcgatg taccaagcca
    15241 agcatacggc tataacgaag aattgatcgg tatccatgtg gctttggcta atagtgctgc
    15301 tgcttatgca aaactccgtc aagctggaca tccagttatt ggattgattg caggaaatgc
    15361 tatttcaggt gcatttttag cacatggatt acaatcaagc cgcttgattg ctttaaacaa
    15421 taaagaaatt accgtccaag cgatgtctaa agaaagtgct gcacgtgtga caaaacgttc
    15481 ggttgctgaa atcgaagcag ctgctgaaaa agttccagct atcgcttacg atatcaataa
    15541 ttatacaaaa ctaggagcga tctatcattt cctagaagat atcaccgacc aagctgcttc
    15601 agaacaaaat gtcagcaaag tcatcgaagc aatcaatgaa gccattcaag atgtccgcaa
    15661 tacaaatgac acgatgctga caaatcgata cacaaacgaa acagccaaaa cattcggacg
    15721 tgtcgaaaca aataaagtcc gtgccaaaat ggacgaagaa tgggatgcat aaaaaatgat
    15781 tcaagcacat gatattgtcc tttttgataa acatgaaata gaagggttca ccttgcctga
    15841 atgggtcgtt ctaactgaaa agatgttcgg aacagttcgt cgtgcgtcga cggatcacgc
    15901 agaaatacta ccgattggtt tacgtggacc tgcccgcaat cagagatggt caacagaaat
    15961 ctcaaaacat gccgtcaaac aagtcattca tccgtgggac atcatcgaac aagaatcgta
    16021 tagacaagag aaaatcatgg attatcctgt ttatcagcaa tttcatgctg ctagaaacat
    16081 attatcagga tgtaaatggg gaatcggcgg cagcttagga tttgaattat cgactgggat
    16141 tccggcagta aaagaaacca gtgattttga tttgctgctg tacgcaaatt caccgattga
    16201 attgccaatt caggtaattc aatcgcatcc tgcttttttt gagcaattcg atacccaagt
    16261 agtgactcgg caaggcggtt tttcactaaa agagtatttg agagagccag agaaaaaaat
    16321 tctattgaaa acagtcgaag gacctaaatt aaccaaagaa ctttggtaat aaagaaaaag
    16381 agagagaaag agatgtttga aaacaaaaaa atcaaaatca acgtaagtga cttccagaaa
    16441 gtatcagctg taacggctgt tatgatgcaa acgatattga gctttgcttt ggcaaacagc
    16501 cacagcgcaa caaatacagc ttttcttggt accttttacg tcatcgttaa atacacagca
    16561 ccaatgttta tttttgcaat tgtttataat atggtaaaaa caagtcagca tttgtcttat
    16621 tgggaatttt tgaaagaaaa gttttttgaa ctagtcgttc cgtatcttct atggacgacg
    16681 gcttatcttt ggctatttcc agcagtccaa caacaaactc cgtatacgga tactgcaagc
    16741 tttttactga aatatgtcac aggtgatgga gcgcctcatt tatggtatac cgttatgatg
    16801 ctgcagatcc aattgttcat gccgtttttt gtttggctcg gttacaaagt atttgctaat
    16861 aaaaaatatg tatggccagt attgattggt tcaacgattt tatatgtcgc atggtatctg
    16921 ttctatcaaa cacaagtatt gactgggcct tatcatgaca gctggtactt attagatcgg
    16981 tttgttgcta gtttcatgat ttatggggta gccgcatttg tttatcacga aaaagtctat
    17041 caatatttgg acagagtacg ttatcttttc ttacctgttg ccttagttat cgcgttcttt
    17101 tctgtccgtt cattgctcgc acatccagga gacttgagct ttgcgaatgc accttacttg
    17161 aatacgatcc aaagtttata cagtttagtc atcatctttg ctgtcttcat aggcgcttcc
    17221 aaaatgattg taaacgattc gccaaaattg ccattgttca aatggttgtc tgtttatgct
    17281 tatcgtacgt atttagccaa tgtatttgtt ttccaagtac tgttattatt gtttaaagat
    17341 agttggctgc agttaccttc aggagtcatg attcttgtag cttatctaat gacggcaagt
    17401 tgtgcatttg ctttaagctg gttacttcat atcatttggg tagctatcaa aaaaggtttc
    17461 agcaagtaat agtaggaaga aggagtgagt gtcatgataa atgacgaacg aaaaaaagca
    17521 gtgactcagc aactcgctgc gttggtagag gaagcattga tcgcagaagt aacattaagt
    17581 ccaaaacctg gattagtgga tgcactggat gcgggtgcac attccgatat ggactatgct
    17641 ttatttctta agtctgctaa gacgctaact cctttttttg aagaaatggc tcaaatagca
    17701 tggcatcatg ctattgatca ggagttaaga gaaagaattg ctgagattgg gcgaaaagcg
    17761 gaaaaggcaa tgctgattgc tacaaatggc atcaataccc ataaaggagc gatttgggca
    17821 atggggctat tgatcagtgt cattgcacaa aaaatcagta tggaacagcc tttttcttta
    17881 tcgaaaatac ttgatgatgt cagcaagctt gctgcttttc ctgatacaaa atatcgtcat
    17941 aaaaaaacca ctcatggtca tagggtgaag caaaaatatg gcgtaaatgg tgcatatgaa
    18001 gaagcgatat tagggtatcc gcatatcagg cttgcaattc aagcagctaa ggagctagat
    18061 aatgattcat atgtacagaa acaaatgcat atgttgttag ttttgatgag ttcgctagat
    18121 gacacatgcg tattgcatcg cagtgatcag aaaacgttag tggaaatgca aaaacttgct
    18181 catgctgcta gtcaaagtgc tttaccaaat cgtccattta cagcactgtt gcacttttgt
    18241 caggaaaacc aaatctctcc tggaggaagc gcagatctct tgtctgcaag cttgttcatg
    18301 ctgaagatag aggaattatg gactgccgac acatacattt gtccgatgtt actcaatgac
    18361 taaataagac aaaattgcta aaaaaacagt ttatgcatct ggttattgac tcttcctttt
    18421 agcaaggcta tcataaaaat agttttttgt aataaaagat aacgaggaag acaatgaaaa
    18481 aaatcaatta tttagcaatc attttgttag tgggcctatg tttgtttgct ctgccgaccc
    18541 aaggattcgc tgtcgcgggt ggtcatggtg gaggtacatc tagcggtcga agttcgggca
    18601 gaggttcatt tggtggtcgt tcatatgggg gcagttattt tggcagtcgt tcttacggca
    18661 gagattctta tgggtattat ggtccaggta caggtccttc tagttatgag caaacgggat
    18721 ttcttgcatt agctgttttg tcagtaggag gggtttctgc tttttctatc gtcaagaata
    18781 aaagacaaaa gaaaaggctg aaagcggaga tattgtcccg catgtcagga acggctaaag
    18841 aaaagaaagc acagtttctg gaaatccaaa agatattcat gaagatccaa tccgcttggg
    18901 aagaagaaac tcctgaaaaa gccagaagct gttatacaga caagctgttt gccgaacatc
    18961 agaaagttct gcaaaaaaac aaagcagacg gtgtaagaaa tcatactaaa aaaataacta
    19021 ttcaggaagt aggaaattat cgacagatcc acgaaaatag ttttgccatc cgaattgatt
    19081 tcagatgctg tgattattca gtcgatcgaa aagatgggcg attgatctca ggaagtaaga
    19141 cacaacgaca gtatttttct cagatatggt attttgattt ttctgagaaa gaaaaaagat
    19201 ggcaagccga ttttattcag ccgattatgc tggattgaca ggaaatattt atgaacggag
    19261 ttgtgacaaa agccttgtcg cagctccttt ttctgaataa acggtgtgcc agagatagcc
    19321 tatcggcaat aaggcacgat tttgtaaaat atgggaagcg atttttcaaa atcgcttctt
    19381 atcgctcaag gctgaacatc tctgtcacaa cctctccact aaatggtgtt taaaagcttg
    19441 catctgcgat aataagaaca accaaacgaa aaataggaag cactatttta gtttagttgt
    19501 tcttattact tgatgcagga cagctttttc acaacctctt ttttgtatag atgggcaaaa
    19561 tttttgtaat aataggcgta tgtctttttc gttgaggaga tgaaaaagca tatggcttat
    19621 tttttgtttg ctttgcttac tttctttcca cttcaagtgg cagaaatatc tactttttat
    19681 tatgtaagcg gaaccatata cttgaaatca gaaggagatc atttctatga aaacaagaac
    19741 aattgaaata cttcacagaa tcatgacagc ccaatatcca gtaaaagcaa aacatctagc
    19801 aagtgagttc aatgtcagtc agcggacgat ccgtcaggaa gttcttgaag cgaatatctg
    19861 gttgagcagt aaaaagcttc cggagatccg tacaattcgc aacaaaggtt ttttgttgaa
    19921 gctggaaaag gaaaagcaag ctcgattaga aactgaaata aaagatgtga aagacggctt
    19981 tttgaatcgg cgcgaacgta tttttgatct agttttagcc gttgcttaca gccgaatccc
    20041 gatccatcta aacaaaaaag aagaagaatt tcaaatctct aaaagtacat tagatgaaga
    20101 tatgagacgg ttacgagcaa cactggtcag ttatgggata gagatcgtca gttatggaaa
    20161 gcaaggactg gtttataaag gttcagaacg atctatccgt acgatgatct atgattttat
    20221 caatcagaat ctgggaatga ccgacttctt tatgaatcaa gatgcacaat taacaccggc
    20281 tcaaaaaatc tttctccatt attttccttt ggtggaaatc aaaaaaatac aggagatcta
    20341 ttccaataag ttgttgaaac aagaagatga tatttataaa aaacaaatcc tgctttttac
    20401 attgatctgg atccagcgaa tcaggaaaca tgaatttatc tctgccatta attggaaaaa
    20461 tacagaaata aaagacagta gcttttctgc atttattgac cacatgatcg aggaatttca
    20521 attggacaat gtcccgcaag tagaaagaaa ttatcttcgt ttcactattg agacgttcaa
    20581 tgctagagat atctccaatt caatcgagtg ggtacaagct caattactaa cgatccaatt
    20641 gatccaattt gtagagaacg agacacacgt acctttccat ctaaaggaag aagctttatt
    20701 cgaaaatctc tataagcaca tggctgcact gattgtaaga atcaagaatc atatccaagt
    20761 catgaatcca ttgaaagaaa acatccgaac aaattatttc ccaatctacc gcgcagtcac
    20821 tcattttgtt cctacgatcg aagaagtaat tggtggcaag atactggagg acgagatagc
    20881 ttttcttgtc atccactttt cgacgatcgc cagtgtaatc aagcgagact tgaattatgt
    20941 ttataaatca gtcgttgtct gtaaccatgg gatggctact ggaaatctgt tagcagagaa
    21001 tctgaaagaa aagtttcctc agatcgagat tactgcagtt ttaagttcaa aagaaatcga
    21061 attagtagat aaattggatg tcaatctggt tttcagcacc tttcaattga attatcgaaa
    21121 taagccgctt ttagttgtcg atccgatttt aactgagacg aatatcttgg ttatagaaga
    21181 ctttttgaaa agaaatgaag aactacagcg gttagagcct aacagtcatg aatcgacgga
    21241 gttattcact caaataatag atgtcatgaa agaaagtggt ggccgagtag atcgaatcat
    21301 ctatggcaag ttagaaaaat tattcgaaac aaacaattta gaaatcaaca aaagggagat
    21361 ccagcctatg ttaaaagata ttttgacgga cagcaatatc atgatcaaag aaaaagccac
    21421 tacttgggag gaatcgattg aactagctgc tagaccattg gttaaagaaa acattgtcga
    21481 agagcgctat gtcgaagcga tgattcgtgc agtgaatgat tacggaccgt atatcgtgat
    21541 tggcaaacac atggcactag cccatgcccg accagaagat ggggtcaata aattaggagt
    21601 cagcgtagct acgattgagc aaccaatcga tttcggtaat cctgaaatgg atccagtaaa
    21661 gattattttt tgtttggcag cagtagactc ctactcgcat ctgaacatca tgaaagaact
    21721 aattgaattg atcaacaacg aagagaagtt ggatcgattg attgaatgca caaatatcga
    21781 agatttcaaa caactgcttt tttcagtagt tgagaaataa taaatgatac gggggagaac
    21841 aaagatgaac aaattgaaca ttttattcgt atgcggtgca ggtttaggaa gtagttttgc
    21901 agcacagatg tctgctgagg atgttttgaa caaattagga gtagaagcca aactggacca
    21961 ttgtgatatt tcttccgcag tttccatgaa accagatgtg atcatcacag cagaaaattt
    22021 tcgttcgcag ttcgaaaagt tcgcagttga tcctaaaacg gcgatgatct atttgaaaaa
    22081 tattgtttca aaaaatgaaa ttgaagaaaa aatcacccca gttctgcaag ataagggagt
    22141 tatctaaata caaaaagaaa agagggtaaa aacatgggcg ttatcaattt tatcatcgaa
    22201 aatattttga ctcaggcatc gatcaccatt gcattgattg ctatgttggg tctactactg
    22261 cagaagaaat cagcaggaca agtcatctct ggcacgttaa agacactcct aggatttcaa
    22321 gtgttgagtg ctggttcgag catcatcgtt ggaagtttaa cttatttcgg gaaaatcttt
    22381 actgaaggat ttcatatgca agggatcatc ccttctattg aatctatcaa tggtcaagca
    22441 atgaatgatt tgggtttagg tagagatatt gcactgacgt tcttagccat ttttgtattc
    22501 aatattattt tggctcgttt cacaaaatgg aagtacattt ttttaactgg tcaagcaatt
    22561 ttatggatgg caacgatgac gacagtcttt ggctattttg ccggtctgcg agggatcgta
    22621 ttgatcttgg tcggtggttt tatcggagct tgtttcgcca ttgctatgcc ggcagtagcg
    22681 caaccgatca ttcgtaaaat cactggatca aatgatattg ctttaggtca tttttgtacg
    22741 atcgggtact tatttgaagc cggtgttgct aagctttttg gtgagaaagg cgaaaataaa
    22801 aagtcgatcg aagatatcaa gttgccgaca catttcgaat ttctgcaaga tacttatttg
    22861 tctgtcatgg tcgtgatggt cccgctatat atcatcactg ttttatttgc aggcgaacca
    22921 ttcgcttctg aattatcagg agatcaaaac tatatcatgt ttgccttttt acaagccatc
    22981 caattcgtag tgggtgtgta tgtattgcta gctggtgtac gtctactgtt aggtgaaatc
    23041 gtgccggcat ttcgagggat agctatgaaa ttagttcctg atgcgatccc tgccttagat
    23101 tgtccagttt tctttcctta tagcccaaat gctgtcattt taggtttcat tacgacaacg
    23161 attggtacga ttattgccat gtttactttg cccatgttcg gcttagcgat gattttacct
    23221 ggtatgctaa ccaatttctt tgccggaggg acagcaggga tatttggcaa cgcagttggc
    23281 ggacgccgag gagcgatcat tggcggtatt gctcatggtt tctttattac attattgccc
    23341 gctttgctag taacgatctt taattcgatg ggattcaaca atgctacagc aaccgatgta
    23401 gatacagtag ccgcagcact cttatatgcc tggatcttaa gcccagtttt gaaagcattt
    23461 taattgaaag tagggaaaat gatgtttgga ttttttaaga aaaaagaaaa agaaccgaaa
    23521 aaagttctgg caaaagaaga aaaagcccta tcgccagaag cgaccgaaaa gattcaatca
    23581 gagattacac aattgaaaca agagatttcc actacccaag ataagcataa attagcgaaa
    23641 ctttatgaac aagttggact taaattttct gaattgtatg tgaacgatca agccatccaa
    23701 tatttagaaa agagtttgga aaataaacag acgatcggag atgggtataa aaaactgatg
    23761 agcctctata accagaagcg tgcagatgct gctcgtgctg gtgatgatca agggatcgat
    23821 tactacatgg gcaaaatgga tgagatgcga caaattgcaa aacaagtaac gatcaaagga
    23881 aacaaataaa aagataaggt ggcaataaaa gatgtataca acattgaaag aagtaacaaa
    23941 aacagcagaa gagttgaatt ttacagttgg agcttttaat acgcataacc tagaaatgct
    24001 tcctgctatg ctaggcgcag caaaagaaat gggttcgccg atcattattc aaacaagtgt
    24061 cgataccgct aaatatatcg gcatgcatgt gttagtcaat gtaatgacag ctattgcaga
    24121 agaagaaatg gttgatgcag tcttgcactt agaccacgcc cgagactttg atgatatcaa
    24181 agaagcaatc gacagcggat atacttctgt catgtatgat gggtcccatc tgcctttcaa
    24241 agaaaatatc ctgaagacaa aagcagtagt agaatatgcc catgcgcacg gtgtttccgt
    24301 agaaggagaa cttggaacaa tcggcggtac agaagaagga atccatgtcg ctgaaaatga
    24361 caaggtatat acaagaccgg aagacgcagt gaaatttgta gaagctacag gcgtggatgc
    24421 attagctatt gctatcggta cgaatcatgg acaattcaaa tcaaaaacag aagtaaacat
    24481 tcccttgctg aaagaaatcg atgcagtagt tgatgtcccc ctagttatcc atggaggaac
    24541 aggtgtgaaa gaagaagatt atcctgaatt gatcaataac gggatccgca aattcaatgt
    24601 tggaacggag cttttagtca actggacgaa agtagctaaa gaaaccttta gtgaaacaga
    24661 aatcaacaag tctcttcgcc ataatgtgat tccagcaaat gaagcagtaa aagaaattgt
    24721 caagcataag attggactgt ttatgaactt ggaaagtcca attaacttag gaaataaatg
    24781 agatgaaaaa gtatttcatt cttctggctg gtagtcctgg tacaggaaaa acatatctta
    24841 tgaataaact acgggaacga tttccagata tgtatgccat cacgctcgat gaaatcaagg
    24901 aatactatgc agaatccatc gggtttaaca atttggaaga acgggcggaa caagaaagga
    24961 cgaaagttta tcctttctat tacaaagcac ttgatttgta tatggaagca ggaaagaaag
    25021 tgattgtgtc cgagtatcct tttagtgata agcaaaaagc acgactccaa gaattagcag
    25081 atagatacgg atatcaggtg atcacgattc gcctaacagc agactttgaa gtgttatggc
    25141 aacgtcggta caaacgtgat cgtgaatcag atcgtcattt gagctatatc atggatcatt
    25201 atcactatgg tgacgtattg gaagatcgaa cgaaagggac caatcatatt acgaaagaag
    25261 cattcaaaaa gatcatcgcc gaccgaaaat atgacaaatt ccagttagga acactatatg
    25321 aatttgatgt aacagactat actcaagtgg attacactcc actattggat aaattagaaa
    25381 aagaaatcaa tgacaataag taacaaagaa atggcttaat atcgaaaaat tggcttttga
    25441 acgctgttta ccgagaggtc gtgagcctgt cgttcagctc cgagtcacaa ggagcaattt
    25501 caaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccgagg agttggtagg
    25561 agaacaccgt ttagtgggga ggttgtgaaa aagctgtcct gcctcaatta ataagaacag
    25621 ccagacaaga atagctcgtc atatttttcg tttggtgggg cttattaccg cagatgcaag
    25681 cttttgaacg ccatttattc acagccccag agaagtcgaa tgtcttttgt tctcgagttt
    25741 tttaacattc ttttatttat tgcagatttc attatattta tttgccgtaa ttaaaccagt
    25801 cttctatact tgattacata gtcatacatt tgctaaaaaa gaagggaaga gaatgaacaa
    25861 gaagttggct caatacaaaa gactactcgc actttctcaa gctggtctat attatggaaa
    25921 agatgtattt gataaagagc gttttgagga gatccggacg atttcattgg aactgatcag
    25981 tgaaatagga aaagaaactt tcgaagaaat aaaagcgctc acaacgattg gagaaggaca
    26041 tccaacacca aaagtagatg ttcgtgccta tatcaaaaaa gatggcaagg tcctactaat
    26101 cgaagataaa cggacgaaag aatggtcact tcctggtgga tttgctgaaa taggattgtc
    26161 tcctgaagaa aatgtgcgta aagaagtcta tgaagaaaca ggattaacag tagaaactac
    26221 tcagttgcga gcagtttttg atacgaataa acaaaaagat attccacagc tttttcagta
    26281 ttataaactt gtttttgctt gtgcgatcca cggtgaagag gccaagttta ttgagaataa
    26341 tgaaacttcc aacatgggat tttttagtat agaagagttg ccaaagctat cagaaaagcg
    26401 aacaacgaag aaacagttac ttattttaga aaacaaaaac cagatctatt gtgattgagc
    26461 tttttcgtaa aagaagaaaa gaatatactt tactagttat taaaaaagaa cttgattttt
    26521 ttattgaaaa tagttgcatt tgcaacaaaa agaggtgaac tagtcttaca aacaaaataa
    26581 aggtgggatg aaatggctga aatccttcga gacatcggaa tgattgcccg agcgctagat
    26641 tctattagca atatcgaatt caaagaagtc gatttaacaa gaggacagta tctttattta
    26701 gtacgtattt gcgaaaatcc tggaatcatc caagaaaaac tagcagaaat gatcaaagta
    26761 gatcgtacaa cgactgcccg agcaatcaag aaattagaat ccaatgggat gatcgaacgt
    26821 ttggaagata aagaaaataa aaaaatcaag aaactctatc cgactaaaaa aggagcagaa
    26881 atctatcctt tcatcattcg agaaaacaac tactccaatg cagtcgcatt aaatggtctt
    26941 tctgacgaag aagcaaaaca attggaatat cttttaaata aagtatgcaa gaatgttagc
    27001 gaagattgga attttgtcaa aaaaggcaat aaaaggatct attgaaagga agaagccgtg
    27061 aataaatacg aaattcgaaa aatgacgcct tcagatgtag acttactaca acaaattagt
    27121 atcgaaacat ttacagatac atttggaaaa gataattcac cggagcattt agctgattac
    27181 ttgacacaag cttatgcact agaaaaactt acagaagagc taaaagataa agaaaccgat
    27241 tttttcctag tgagtatcga tcaagagcct gcgggttacc tgaaactaaa tacaggaacg
    27301 agtcaatctg aggaaatggc tgatcatttt ttagaaatcg agcgaatcta tgtacgaaaa
    27361 aaatttaaac atcaaggtat cggtacattt ttactagata cagctattga aaaagcaaaa
    27421 gaaaagcaaa aaacagccat ttggctcggt gtttgggaac acaacgatgt tgcgctcgct
    27481 ttttatcgta aaaaaggatt tatcaaatac agcgaacatg ttttttatat gggagatgat
    27541 ccccaaacag actatctatt catcaagcaa ttattctaat caagaggtgt gaacagatga
    27601 aaaaagtaat agtggatacc aatttctggg aagtatttcc tgaagcaacc atcgaaatct
    27661 tatccgtaaa tggtatcgat aaccacgtaa caaaagaaag tgaagaaacg tatcatcaat
    27721 tattgaatga ggcagcaaaa gaagcaagaa attaccttac agaagaaaca ttcagccaaa
    27781 acgaagtcat tgctcagtgg cgtcaggcat ttaccacatt caagactaaa aaaggtgcac
    27841 gttcttctat tgaagcttta ctgaaacgtg caaatcaaga acgggaattt ttcccaatca
    27901 atccgcttgt agatatctat aatagtattt ccttgtcttt tggcgtacct tgcggcggag
    27961 aagatatcga aaagatcgca ggaaatctgc atttaggaaa agcccaaggt ggcgaatcat
    28021 tcctgccttt aggtgcagat tccgatgctc cggcacttcc ggaagaaatc atctactatg
    28081 atgaacaagg agcgatctgt cgctgtctca actggcgtga agcccaaaga acgatgctga
    28141 cggaagaaac acaagcagcg gtgctggtca ttgaatccat caatgctgaa caagcggcac
    28201 gtggcgcaca ggcaatcgaa gcgttgaagg aaaaaatcga tcaaacattt ggcgtaacta
    28261 gtgttagaca cacactaacc aaagatgaat cagaagcaga attataaaag aatcgaaata
    28321 a
//
LOCUS       DAXWLH010000017        27873 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXWLH010000017 DAXWLH010000000
VERSION     DAXWLH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.017"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(959..1132)
                     /locus_tag="ACGE36_000538"
     CDS             complement(959..1132)
                     /locus_tag="ACGE36_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382961.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1135..1533)
                     /locus_tag="ACGE36_000539"
     CDS             complement(1135..1533)
                     /locus_tag="ACGE36_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382962.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1749..2804
                     /locus_tag="ACGE36_000540"
     CDS             1749..2804
                     /locus_tag="ACGE36_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382963.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2824..3177
                     /locus_tag="ACGE36_000541"
     CDS             2824..3177
                     /locus_tag="ACGE36_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382964.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3359..3646)
                     /locus_tag="ACGE36_000542"
     CDS             complement(3359..3646)
                     /locus_tag="ACGE36_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382965.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3659..4477)
                     /locus_tag="ACGE36_000543"
     CDS             complement(3659..4477)
                     /locus_tag="ACGE36_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8382966.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4703..4924
                     /gene="relB"
                     /locus_tag="ACGE36_000544"
     CDS             4703..4924
                     /gene="relB"
                     /locus_tag="ACGE36_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8382967.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4926..5192
                     /locus_tag="ACGE36_000545"
     CDS             4926..5192
                     /locus_tag="ACGE36_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8382968.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5359..5844
                     /locus_tag="ACGE36_000546"
     CDS             5359..5844
                     /locus_tag="ACGE36_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382969.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            6053..6235
                     /locus_tag="ACGE36_000547"
     CDS             6053..6235
                     /locus_tag="ACGE36_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8382970.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6338..6622
                     /locus_tag="ACGE36_000548"
     CDS             6338..6622
                     /locus_tag="ACGE36_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382971.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6638..6874
                     /locus_tag="ACGE36_000549"
     CDS             6638..6874
                     /locus_tag="ACGE36_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382972.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6896..7150
                     /locus_tag="ACGE36_000550"
     CDS             6896..7150
                     /locus_tag="ACGE36_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382973.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7232..7417
                     /locus_tag="ACGE36_000551"
     CDS             7232..7417
                     /locus_tag="ACGE36_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFF8382974.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7484..7879
                     /locus_tag="ACGE36_000552"
     CDS             7484..7879
                     /locus_tag="ACGE36_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8382975.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7896..8246
                     /locus_tag="ACGE36_000553"
     CDS             7896..8246
                     /locus_tag="ACGE36_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382976.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            8268..8642
                     /locus_tag="ACGE36_000554"
     CDS             8268..8642
                     /locus_tag="ACGE36_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382977.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8949..9347)
                     /locus_tag="ACGE36_000555"
     CDS             complement(8949..9347)
                     /locus_tag="ACGE36_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8382978.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9539..10306)
                     /locus_tag="ACGE36_000556"
     CDS             complement(9539..10306)
                     /locus_tag="ACGE36_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8382979.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(10341..11501)
                     /locus_tag="ACGE36_000557"
     CDS             complement(10341..11501)
                     /locus_tag="ACGE36_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8382980.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            12545..13348
                     /locus_tag="ACGE36_000558"
     CDS             12545..13348
                     /locus_tag="ACGE36_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8382981.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13933..14388
                     /locus_tag="ACGE36_000559"
     CDS             13933..14388
                     /locus_tag="ACGE36_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382982.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14507..15565
                     /locus_tag="ACGE36_000560"
     CDS             14507..15565
                     /locus_tag="ACGE36_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFF8382983.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15607..17556
                     /locus_tag="ACGE36_000561"
     CDS             15607..17556
                     /locus_tag="ACGE36_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8382984.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17559..21200
                     /locus_tag="ACGE36_000562"
     CDS             17559..21200
                     /locus_tag="ACGE36_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFF8382985.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            21600..21938
                     /locus_tag="ACGE36_000563"
     CDS             21600..21938
                     /locus_tag="ACGE36_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382986.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            22024..22269
                     /locus_tag="ACGE36_000564"
     CDS             22024..22269
                     /locus_tag="ACGE36_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382987.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            22289..24445
                     /locus_tag="ACGE36_000565"
     CDS             22289..24445
                     /locus_tag="ACGE36_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382988.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            24457..24690
                     /locus_tag="ACGE36_000566"
     CDS             24457..24690
                     /locus_tag="ACGE36_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8382989.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            24680..25546
                     /locus_tag="ACGE36_000567"
     CDS             24680..25546
                     /locus_tag="ACGE36_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382990.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            25561..27531
                     /locus_tag="ACGE36_000568"
     CDS             25561..27531
                     /locus_tag="ACGE36_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8382991.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         9994 a         4239 c         5011 g         8629 t
ORIGIN      
        1 tttctttaca ttcctacgca ccttgcgtgt aaaatttctt tattcctccc acgctccatg
       61 ttattttata tttccttttt catgttattt ttatccagaa accgaacaag tttttaccta
      121 ttcccaaaat taatttatta gttatttttt cattgattaa gaataattat ttgatgctta
      181 aattagctaa aaaaacgatt ttttactgat ataatcatga tattaaagct gaattaggcg
      241 gaaagataaa atgttcgcaa aatcaaagta tcaattatct tgttttttta taaatttgta
      301 agtaaaaatc taaaaagttt tcttttaatt ggtatatgta ttaaaatcac attcaatcac
      361 tcataaaagt atgcgctttc atttttttgg atgagattaa tatcaataaa taacgatgta
      421 aaaaccatta attatgctct tttataagga tattaattaa tctaatttga aaaaataatt
      481 caaaatttaa atgttttaat attacaaata aaatcatgtt atcgtaaaat ggtataaaaa
      541 agctaaatat tctatttttg ttattttatt agcagggaaa tttaaatagt ttacaagaat
      601 ccgtagaaat atattggttt tgccttcaga ttaatactgg atcatattaa tctgaatata
      661 atatatgaac taataaatag cttaacctcg ctattttgtt ttttctgatc actatctata
      721 cagcattagt aaatagaatt ttaaaaataa aaaaattaag acaaaaaaag tatcaacata
      781 aacatataca tatacacgtt tatgttgata cacttaattt gtcctttaaa aagcacttat
      841 tttaatatta tgttatttta taatcttatt acaagctgat tgttagacgt tgtcctttca
      901 ttacatttat taacggttat tttatcgttt aatttattaa ttatttttga tctaaaactt
      961 agcttaacaa ataaataatt aaccaaccgc cagataaaaa tgcgatcatt gctaatacat
     1021 aagcaaagat aaaaatatgt tcgatcagct taaaccctga tgcgattaag cagaccaagt
     1081 aaaatagaaa ttctcttaca gataatggta attttctaac tagcatactc ataattaaca
     1141 ccccaaagta atattgtatt ttgaacattt tacatccaat acagcatcaa tcaaccattg
     1201 ccaatcaaaa atacaaggct tcattgaagt atgtttttca gatgaaaaga tataatataa
     1261 tttcttcgga ataatatttt cagttgttgg gatcaagcta aatttaatac catctaactc
     1321 atagctacca gctttctctc cattaaaaat ttccttagca ctttcttcta aagaatcaaa
     1381 atatttttcg acagcaacct ttgaagcttt tactattgaa gcatttccct tttcgataaa
     1441 atctgctttt gttttaccga caataagtaa ttcttgatca gtaaaatctt ctaaaataat
     1501 agtagtgtaa ttcttatttc ttctcaatcg catcaggatc actccttaat ataatttatg
     1561 cttttataat atcataataa gataaatttg caagtgttta tttacacctt tttatttaca
     1621 ctttattgta attgattata cttttttgat tgcatttttt tcttataaac agttgcaact
     1681 attagtaaat aggtgtaaac tattttcgta ccgaaatacc ataaacacaa acagaaagag
     1741 gtcataccat gaaagaacca ttattgtttt ccgttgactt agggaataaa tcagtaaaag
     1801 aaatgagtac acacgtttct ccactctcgc ttccctctcg tattttgaac aaaaatatca
     1861 ttaattcagg acgtttttct aatataggtt cctcattaaa acgatcacta aacgtttctg
     1921 aatatagcct agtaaacact actgaaagct attattttgg gaaggatata cacgagctag
     1981 gaaaagatga ctacgtgata gaatcactag gttttgggat cagcagatac aagcaggaaa
     2041 cctaccaaga tatcctatct ttctctttag ctaacctagc acgtttagaa gatcaatctg
     2101 atgacgtttt aaccgttaat ctagttttag gtctgccttc atccgactat accgaaacaa
     2161 tcgtcaaata cgtgatgcaa caagccacac gacaacattc agttgaagtt gacggtaaag
     2221 cttataatat aaacgtagat accgtttggg tactgccaca gccgattgga acactatata
     2281 acctcctcct tgctgatgat ggtagtgtac aagatgaatc attgatcagt cagaagatcg
     2341 gttttgttga tattggtggt ggcacgaaac tttttgatgt tgtccaaaat tttgaaattg
     2401 acgaaaacaa ccgtgaacaa aaggataccg gtatttttac tttatataat cgtatcgtaa
     2461 atatgcttga ccttccgatc agtcctaatg ctttccaact tgaaaagatt atccgagaag
     2521 gcaaagaaaa aggcgtatat ctatttaaac caagcggaaa tgaaagtttt gatatttcag
     2581 aagtagttga aagagaaata aaaacataca ccaatatggt tgttgcagaa gttttagcta
     2641 cattcaaaaa cttcacaacc ttagataaag ttgtctttac tggtggtggg gcttctatta
     2701 tcgacaaaca acttgtaaaa gataaaattc cgcatacctt gtttgttgaa gacagtgaat
     2761 tttctaatgt aaaaggcttt tacaagtttg gtttaactca ataaaacagc gaaggagatc
     2821 aacatgaata agggaaaatc agagcgttat gtagttataa tcaatagaga aaaggttagc
     2881 gagttacctt tagcagagtt tcttgaatct gaatatgaaa cactaggcgt taaatactct
     2941 cagacgatca aaaaagcttt gaatctatat cggcgtgtaa agaacaatga agttacaatt
     3001 attgaagcag gcaacgatag aaacgatagc aaagtagctg aagtaaagaa taaaccaact
     3061 gatcataacc ctattcaaca agcgcatcct tcaacggtta catccgatca ttcagcaaag
     3121 aaagaaaagc caatgctaaa agggttcaat gcaccaagta acaaagactt actttaaaaa
     3181 taaaaaggcg aggttacaat acctagcctt tttattatgt acaaatacaa acataaacat
     3241 atacgtatac atgtatataa acatgtatac gtatatatcc ataaacacgt atacgaaatt
     3301 tagttaagct atacctaacc gcttattcca ataaaaaaag tgatcagctt taaactgatc
     3361 actcaccaaa aacttcatca tacaagttat ctatcatttc accaagtaaa gctgaatctg
     3421 atcgaattcc taacttttcg ctgattttag atagtttctt tctttgtgat ggttgaagcg
     3481 tgaattgata atttttcttc ttttcagaac tatcactttt tagtttagct accaaagaac
     3541 ttgttgtttc tttaggtgtt tgtttaggtt cttcaactgc ttcaaccact tcaggaactt
     3601 cttctacttc agcttcagcg aattttttaa atctattttc tttcacttca atcactcctt
     3661 attcttgtgc cgtatcaatt attttgttgt atagcgtaaa cgtgctttca aagaaatcct
     3721 tattcttacg atagatattc ttatccttag cgaactcagc aatattctta ccttctaagc
     3781 tagttgcatt aagcaattcc ttctcttgta catatccaag ataattagat aacgttggta
     3841 ccaattctag gaactcacga cttgaattag tattatgttt gatcctgcta ccgatcgtag
     3901 cgtatttagt ttccatcagt gtttcaccag tcttaatatc aatcaactgt tttgctagtg
     3961 catcaatatc tttaccaagc ttaagcaaag cttcaaaacc atctttagat ggatcagaaa
     4021 tgcctacgac aagatcagat acagcccaag cattttgagc tagtagacct gcatcattat
     4081 gagtatcaat cagaattaga tcatacttct ctttaagctg ttcatagtta acaacgatcc
     4141 atctaaacag tagggttctg ttgttaggca tatcacgcat ttgcatctca acttgtttta
     4201 aatcttcacc tgctactaaa atatccatgt tctcatttat agctaaaggt tcagctgatc
     4261 catgaagata taaatgattg attgtattct ctcttttcgc tttactgaaa gcatcaggaa
     4321 atcgttttga taggttttgc gacttatctt catcaatata cagcaccttc atacctaaat
     4381 tttcgacagc atatccacct agattgtaga ttgttgttgt tttacctgtt ccaccttttt
     4441 caatgtttcc agtaatgatt gacaacttct tttccatgat taaacctccc ttattaatct
     4501 tttgtaactc cattatacac tataaacacg tttatgtata tatttatata cataaacgtg
     4561 tttatattta tatttgcttt tatattctac aacataaaca tatatatgta tatgtttatg
     4621 tttatgtata tattcaaata tataactatt gcatttgtca tacaatcgta gtacaatgta
     4681 attgaaaagg agttgataaa ctatgccagt aataagtatt cgtgtatcta aagaagaaaa
     4741 agattttctt gaaaaagttg ctgaattcaa cggagataac atttctgaat ttatgcgtag
     4801 taattccctg caaacagcag aaacgttagt agactttgcc acctacaagc aactaatgaa
     4861 agagcatgag gaaaacgatc aaagtatttc ccacgaagat atgctaaaag agttaggctt
     4921 ataagatgac ttaccaagta agatatgaac gtgaagctca aaaagcgcta aaaaaaatgg
     4981 ataagtttca ggcaaaggtt atcctaaact ggatcgagaa aaatcttgta ggcactgatg
     5041 atccaagaaa gcatggtaaa ggtctaacag ctaataaatc aggctactgg cgatataggg
     5101 taggtgcata ccgtataata gctgatatta gcgagcatga agtgacaatt cttatattgt
     5161 ctattggtca cagaagtgaa gtttataagt aacagacaag ctgataaggc ttgtctgttt
     5221 ttttgttaac ccaacaaaat cactgtttac gatcccactt tacctaaaaa agaacaatat
     5281 cctcttgata ttatacacac cagtgtgtat aatataagtg taaacaagct aattaacttt
     5341 caaaaggagc gattttttat gggatggtat ggtgattatt catcagcaaa tgaagtgatc
     5401 gaagaaaaga aagggtacgc caaaaatggt ttggaaatcg taggtaccaa agtaacgaag
     5461 acctatggaa ttatcctttt caaagatgca gaaggaacct acgatattga cagcttcatc
     5521 tttcgagatg aaatgtacaa accaatctac ttcttaaatg acagtcctgc ttactttaag
     5581 aaaattccga aaaaatggtt gaaattcttt gtcggtgatc aagaaaaaat ggttaagcta
     5641 caactagata gaattaaaga agaaagcaat aaaccaaaac ttgatgactt attagttgca
     5701 ggtaaaactt actcaatctg ggatggatcg tatgtcgctg tctatcagta taaactcaaa
     5761 cgtagcttta tttttcgact acaagatgga tcactcactc ggttcactcg actatcagca
     5821 ggagatgtta aggaagtaac ctaaaactta aatacataaa cataaacgtg tatacgttta
     5881 tgtttatgta tatgtatatg aatataaacg tattaggtac gaacactttt aaattctttt
     5941 ctgggctttg tttttagggt agggctaaaa aaaacatcac catctgatcc aaaacagctc
     6001 agaaacgcat ttaaacgtat ctgataccaa tattacagaa ggagtgtatc gtatgattaa
     6061 cctatttctt ttagtgacag ctagtacatt attctttact ggaatatcac tgataatttc
     6121 ctatttgatc tataagaaag ccgaaaatca cacaagcaaa gcaatcggac tagccgtttc
     6181 atccttttta actttgctga ctttatttgt tactttatcg ttttcttttg tttagaacag
     6241 ctgatccatt agcacctttt tctctttaca ttatacacac tgttgtgtat aatgtatttg
     6301 taaacaagct aattaacttt taggaaggag tgacaagatg ctaacagcta ctttacagca
     6361 acctactcac gttgctattg cagaaagtta tacaattaat gcagggatcg ttaaacagct
     6421 gatcaaggat cgagaattca aaaagtttaa cagtcggcaa gcgtataaca gacagctaaa
     6481 gaaagctatt ctaaagcaca aagattcctt catcgggaac ttggaaagtg tagatgttaa
     6541 aactgtttca acggcatttt cagttgttga agtgactatt gacggtcgct tgatttctct
     6601 atttgctttt ggaattaatt gatagggagt gtgaactttg gaaggtaaga cttttgcaga
     6661 aatttggact gaatttattg aggtaccaac tcgcattgat ccgataactt ctgaaaaggt
     6721 attggatgca gacttctaca atctcaaagc aggtactagc cttgaatgcg tttggatgta
     6781 tttagaagag catttcaaag gcgtaacgct cggtcacgtc ttataccaca atggtgccga
     6841 gtgggatgca attggaaata tccatttttc ttaatataag aaaggagaac acaatatgaa
     6901 ttataccgat gaagaacaaa tcgctttgga atacgttttc acaaatgaag aaggggagga
     6961 aattgaaaaa gtcttgatca tttcatcaaa agacttggag aaggctttgg cttatgaacc
     7021 atacgaatca accgtaactg tcattgaatt cttagatacc tatacttcta atgatacttg
     7081 ggaaattatg aatatttgca acgctaataa cattcaattt gctgaaaaaa ccaataaatc
     7141 attttattag ttttgcaata aaacatcctc tttttctatt gattccatac acaacaatgt
     7201 gtataataga ttttgtaaag ggagggtagc tatgcctaag acagcaagag aaattgtaaa
     7261 aattctcaaa tctaacggct tcaaaaagac tggacaaact ggcagtcatg caaaatttta
     7321 taattccaag actaaaagaa cggtcgttgt tccgatccac aatggtgatg taccactagg
     7381 aacagaagct aatatctgga agcaagcagg gctttaacag ccttgccttc ctatttggta
     7441 ttaggcatac tatcctctct tacactagaa aaggagcgag gaaatgaaga caaaaaatat
     7501 tttttatccg gcaatcttcg agaaagaagg agaagcttat aacatcgttt tccctgactt
     7561 accggaaata tctacctttg gaaatagtct tgaagaagca tatttaaacg ctaaggatgc
     7621 tttaggatta gcgctatata gtgtaccaga tgcagaattt cctaaaccat cagcagtgga
     7681 agaattaact ttgaaagcca accaagtggt agtaatcatc caactaaaca ttaaactttt
     7741 ccgaagaagc ttaaatacaa aaacaattag aaaaaatgtt agtgttccgg aatggttagt
     7801 tcttttaggg aaggagaaaa acataaactt ctctcaacta cttcaaaaag cactagaaga
     7861 agagttgctt gaaaagtaaa aaggagaccg aacctatgaa agctacacac ttaaactatt
     7921 tctctcgaac gttaatcaat aatctattaa aagatacaac tggcattagc gttagtacca
     7981 aacactttgc caatgatcta attatttttg aggataataa cttgcgaatt gagatgttta
     8041 ttgaacaggc aacccctaac tcaatacctg aattctgcta tacgcacact gaaaaaatga
     8101 aacatgcaaa atcagttaaa gatgttgaag cattaagtga atcactaacg aacgctttaa
     8161 acctcgttaa gaaggcaaaa ttgttatttg aatttcaaac cgactaccac aatgagttgc
     8221 ttgctcaatc tgaaatttta gattaattga aacaggagaa gagaaagatg aaacaaacac
     8281 aaacagaaac cgttaacgat acgcttgctg atcaattagc aggttattat aaattatttg
     8341 agatgggtaa agcacagcgc ttgatccact atctttggaa atacaaaatt ttgtttcttt
     8401 tttgggaatc aacactaaca ttctcagttc ttttttataa gtcagatttt tataatcaac
     8461 acttagtttt agggagtttt ttgcttatct acaccatttt gatgcttaat ttatatacct
     8521 acatcacact tgaagaacac ttagtaatta gcgatgcagg gatcgagaga aacatcaaaa
     8581 gggtatcctt caagatcacg ttaaacaagc ctaccgataa aaagctctca gaagcgttct
     8641 gagagctttt gacattttct tgtataattt tatcctaaca cattacaaag cacctaattc
     8701 gagcttatat caagcaaatt tgctatttat tactcaatac atatctaaaa attacttgat
     8761 ggaaaattag atacatggta agcatgaaaa gaaaatcaat ttgagcagat gcagtacaat
     8821 acatagcaat ttaaaagctc tcagaaggct tctgagagct tttaatatat tctttgataa
     8881 tttgtctttc ttaaatccat acgtcctaaa acaagctgtt tttgcttaat atgatcatct
     8941 gagagacatc aaatcaactt ttctctcaac aaatcattca aagctttcga cagattaata
     9001 ttctcttcag ccgctaaaac atttaaatag tgttccacag tcactgattt tcgatcataa
     9061 ttttgcttgt ttcgagcttt agcaactttc gtattagctt ttacgacaaa taaattaaca
     9121 ttctcagatc gcttgattct tgcatttaac tcttgaacag gcgtaggcga tggaatttct
     9181 ttttcttgca actctaaaag caccaagtat ccttccaata cttcttgcgc catctcaaaa
     9241 gcatcttcta aagtttcacc atgtgtatat aagtctggaa aatctgggaa tttaactgca
     9301 tattcatttc ctttccattc gaatactgca caaaaaaagt atttcatcgc attgcacctc
     9361 tctaattaaa aaataatttt tgcttttttg tttcaaaaaa aagaatcgcc taccaaaaaa
     9421 attgataggc gatcacaagc taattaacct ctaaaggaga ttaatacctt gctcccaagg
     9481 cactatatat cttagcatac gtctgaagca tcttcaaacg agtaagatat attttgcatt
     9541 aacctaattt tttttctcta taagtttttt catcaaactc actagaaata ttaaactgga
     9601 cagcaaaatt gtaatttctg ccttttctaa ctttagcaaa atcaatattc agcattgatt
     9661 tttcattaat ttcctcaact gattccctta aaacacgtct atcaaaattc gatattgttt
     9721 tatactcatt ttctgttttc gtaatctcac gaattttaac tatttcaatc aggggattct
     9781 tatatatttc agctttctca aaatctcctt tatttttggc gataagatat tttttataat
     9841 acatcgccaa atatttgtat aacacgatgc tatatttttt ttgaaaagat gctaagtctt
     9901 caaaaacaaa gaacggatac ttctttttga ttgagacaat taagggaatt acatcttctg
     9961 aaaaactgaa agatacacta tgctcttcta aatcccaatg aatccgatca atagcagata
    10021 cagtagtata cgtcttatat tttttgtcta gattcgaaat ttcataaact gcttgtcttt
    10081 gcaaagtgtt aactatggtt tttagttccg tttcaagatt ttgatcaaca tccatgtaat
    10141 taataatcgc tttaacaggt aaatctacta cgcctgtttt ctttagcttt gtgggtgaga
    10201 tataactgaa cagcaattga taaagttcat acgactttaa atccattttc atgtcaattg
    10261 ttgctaagaa ctcctcattg tactttgtca gttcatcttt tttcaaaagt ttttccttcc
    10321 ttcacgcatt ttttcagttg ttaatcataa aggttcacat aaggcaaata actcgtaatt
    10381 gatttttcga gatatttaac agtatttttt attgtgtaat cctccttatg agtagcaacg
    10441 taagataggt gattgacaac tgattcaaat cctctatctt tggataaacg atcataaagc
    10501 ggataaactt tattctgaag tcggctcata agtttaagat cgcataagtc ggcagaactg
    10561 atcaaaaagt ttttgattag taatttagtg tatttgctat tcatagcttt caagtacgat
    10621 tgattcattt tctctgtttt atcttctgcg ccttttaaat aagcagggtc tctttgttct
    10681 tgcttataag gtaccggaac tgacatggta cgtgtaagca agaactttat gtttgtataa
    10741 gtatgaccct tcttgataag ctcatagtca attttaaaat cagtgtattt attcagttgg
    10801 gcaatcggtt ctttaattac acgtctttca aagtctcgaa aagctttata ttttttctca
    10861 atactaaatt tctttcgtaa atctgcaatg gagaaaacag gattttttaa tctatcaagt
    10921 gattttgtac tccatgttaa gttatctcta tttgcttcat acagattaaa atctttagtg
    10981 aataattgaa aaaaactgat cgcatacttg cttgtcaagt taactacttg atcaaatgta
    11041 tattgcgtaa aatgattttc taagttcgtt aataaaggca taattgaact actaaattta
    11101 atagatactg catcagaaaa atcattgaat tcagtaattt ctagcggtga gatgactttg
    11161 tattcagtct taccgttagg tttagttctt tgaattttaa tgacagattg ctgatgcaat
    11221 ttttcaataa cttgttttaa tcttgaatgt tttgaagaag atgaactaga agacatcaac
    11281 gcaaataaag cctttttaga tactttgacg gtattattaa aaggcggatt tttagggtca
    11341 agatgtccta tagcgtaaag ataaattttt tgtggcatag catccatttc aacaatgcta
    11401 tctacaagtt gattcgataa agtaacagtt aaatcaaact tttttagatt gctttcggta
    11461 gtttcaaatt tcgggtttac attcttttct gttattttca tacgattgcc tcttctttct
    11521 aagcttggta cgagcttaaa aacttggaac tggtacgatt ccattgaata agtatagacc
    11581 tgttttcgat acgttacaat aagaaatgta tcgtaaattt ataacataac tcaatgtcag
    11641 taaaacacat tttatttttt ccaatcaaat ttcattgaaa atacagcaga aatcgctatt
    11701 ttagaacatt ttcaaaataa aaatcctttt caataaaata cgaaattata aaaattacca
    11761 aaattaaaaa acaaatattt ttctagtttt tagaaataaa ttcacatact ttttcttttt
    11821 ttggatatat tcggaatacc acatcgcttt ttgtccacgt ttgcatcgct ttttgtccac
    11881 attcacatcg ctttttgtcc atgttcgcat cgctttatgt ccaccctaaa atctatttaa
    11941 gattgatata aagccttttt ggaggttgct aaagttttaa agattaaaat atatattata
    12001 aatataaata aaaggggaag ctgaagcttc ctccttttcc tttttttcaa ttattcaaag
    12061 ttacacaagc aaaataaact cctagatagt gttagttttt tttaagattt tttattttta
    12121 agattttgaa ataagagagg agtaatcctc ctttattaaa tatctgttaa tatatctgtt
    12181 aaatatctgt taatatatct gttaggggag gcttaatctc ttgggagagt aagtattaaa
    12241 ggggtataaa ggtaacactt ttggacagta aaggtaacaa aagtggacag taagggtaac
    12301 agttttggac agcaaaggta acaaaagtgg acatttgagg tatcacacta ggataagacg
    12361 ttaccttcgg ttttcttgat ttggaaggcg aaaaaataga aaattagatt agtaacaaat
    12421 ctttgttgac tgttaccttt agttgtgatt taatttaggt aacaagcaga aaaaattgtt
    12481 tcctttaatt agtaccagat taacatgatt aaaggagcgc aaaaagaaga ttggagagta
    12541 atcaatgtct caagaagtta ccgaaaagat tattgatagt gatgtagatt tagtatcaga
    12601 acaaacaaat gcaattgtgc gttataacaa tggtctgaat acaacagctt tgcgaggatt
    12661 taagcctgtt gaaatggatg tgttttgggc tattttatca aaaatgaaac gtcaaggaac
    12721 agcagaagta acttttgatt tcaacctctt tcgttatttg gtcaaatacg acagaagaga
    12781 aaaggacagt ttttattctg tgttaaaaga tttgagtgac aaactaggta atttaaccta
    12841 ccgttttgaa gattcagacg tctatgaaca gctgtggctt tttcaaaaat tccggataga
    12901 taaacagaag gaaacagtta caattaaggc aaatgaggaa tttgaattta tcttaaactc
    12961 aattggcaaa aactttactc gttttgaact tgataatttt acaaaactaa gaagtagcta
    13021 cacaaaagaa ttgtatcgtc atctcatgca atacagaaat aaagagtggc gaaatggtta
    13081 ttgggtagtc aaagttgaag attttagaag agcattaagt ataccttcaa gttatagaat
    13141 gacaaatatt gataaaagaa tactagagca ggcgaaagaa gaatttttag ctcctgatga
    13201 aaacggaata caggtatttg aaacttttga ttatgaaaaa atctatgcaa agaaaggaag
    13261 aagagttgat cggctcagat tcacttttag tgagccagaa tcaaacttgc caaccatttc
    13321 aatgcataat tggttagaag aagattgaga agaagcaggt tgtaatttta atacaacctg
    13381 cttctttaaa aaagtatggg taacactttt ggacaataaa tgcaagaagt ttttttgtaa
    13441 gcaagtttca gtctaaataa tatttgaaag aatggctaat ctatacaaat tttaacaact
    13501 atgggtaaca cttttggaca agtcaagaaa aaaaacaatt tttcttaatt tgttgatcga
    13561 ctaaaggtaa cacttttgga caacacagaa taaaaagtcc ggtgatatca gttttggtag
    13621 gaaacatctt aatatctttg tgagattgaa ggtaacattt ttggacaagt caactatttg
    13681 ttaaaagtta atatagagta attagataag ttttttcttt tcgtctgata aatggataat
    13741 attagttatt ttattgctaa ttagtctttc atgatgactg aaatagctaa aataaatatc
    13801 tcgttggata attgctgata ctttaaggga tatctaaatt tgctgattga tttatttatg
    13861 ttattatgat attataataa cataaatgtg ttacgcccga acctcatttt aaaaaaatga
    13921 aggggtgaga caatgaatgc tattaattgg catgaatttg ttaactatat ggagagctta
    13981 ctagatgatc taaaaagttt tagtattgct gatgcagtaa agcatgggtt accagttagt
    14041 acaacgatga aattacgagg attgaaaatt ccagatgaaa ttattaagct gagaactaaa
    14101 acaattattg aaaccgcaaa agtcgttgac aatatgaacc atgaactaaa aggcaagaaa
    14161 acaacttacg agaaagattt tgaacgtttt ttaaaatctg ttattggaaa atatctaggt
    14221 aaaaaatctt tcagtgaatt gagagatgaa gagattgtac ctactccaat ggttagcaaa
    14281 atccgaaaaa acaaagccga ggggaattac tatcttggta caatagcaaa aatcattgat
    14341 tatttggaaa ataaagagca aaagaaagga acgaagaaag gaagataatt tttgtgttaa
    14401 atattaatgg ggaagaatat accgaaatga ttcaaaatcc cgaacctaga attaccgaaa
    14461 aaacgtctga ttgtttgctc ttttatgaaa gggggcagac aaactaatgg aaaaaaacgt
    14521 caattcaaca tctaccaaag atcaatacag tattattgga gggttgcttg gtgataagga
    14581 taagagaaca cgtcacggat tacgcattat gcttgcaact tttctaggag tgcttttttt
    14641 attaacaggc ttgggtagct acatttatca attaggaaac tataattatt atctcgtgaa
    14701 caatacaaca cctataggct cttcattgga gtttgctcga tcaggagcag agatcactat
    14761 ttcagatgtg tggacagatc aaaacagaga tgtaacagta gtaaaactcg gttactcgca
    14821 aaacgctaga aagttgttat ctacgcaagg aaaaaactac aacttatata tggcaacgaa
    14881 agaaaaagat cgtcctaaaa agcttgaaat gagctatggg atgttaagta ctgatgggga
    14941 tgctttctta ttcctaaaag gtaaattaga tgaaagagct tatcaagttt ttattgcaaa
    15001 ccaagtatca ctaatttctg gtgatgatac agctgacgat gataccaaag tcaatgtgaa
    15061 tgaagcagat tcagttacaa aagcactttc tcaatattct atgaatgatg ttaataatga
    15121 aggagtaatt tcttttggga aaaaagcaaa acctgttaat actcctgata atatcaattt
    15181 cagaataaat ccatattcag attcaactaa tgtctatcaa ggaagctttc ttaccaaaga
    15241 tggcgatatt gattattcaa aagttgtagc agttacaagt aagaataatg ttgtaagtaa
    15301 gttagaagat cagttaaatg cttcaaatac taacatcaag aaaattgaag ctacaaaagc
    15361 tgagtatctg gaacgtttac aacaaaatcc tgacgataaa gattcgcaag aaagtattcg
    15421 tcaactagac agtcgaatgg agaccgaaat taaagcaatc aatgatacac aaaaattgat
    15481 tgatcaatac aaaaatgctg actttaacaa agattctttt ggagacatga agacgacatt
    15541 taagtaccta actctgactg actagaagta aattattctt attttagagg aagtgaacac
    15601 aaacagatgg aaaatagtac cgagtttttt aagaatattc aaactgaatt taatcatgct
    15661 ctcacaccaa tgaaaccacc gaaacataag ttagtgggac gtgatgacga actccatgag
    15721 ttgtcactga tcttgaatag accggaaaca ccgcaagcta tcttgttagg tgaagccgga
    15781 acaggtaaaa cagcacttgc aagtgaatgg gctgaaagac agcgacaaga tggaaagaaa
    15841 gtacaagtat acaaattatc aatcggacaa ttgcgtgttg ggggagatga tgtcctccaa
    15901 acacgtttag aaacattaat tccaagattg aaagaatatg agcgaatttt aaaatctgag
    15961 gacaaagaaa atgaatttgt cctcttcatt gacgagtttc ataaagttgt tagctctttt
    16021 ggtagtggca ataaaattgg gggtgattcg ctaaaagaac cgttagcaaa tgaatcactt
    16081 caattgatta ccgctactac aagaaaagaa tatgaaaact atatcaaaag agatgatgcc
    16141 ttagggagac ggttacaacc gttagaattg aatgaactaa aaagcgatgt tgtcgtcgat
    16201 attttgagac aatggctaaa tacatcctct gttaaagatg atcctcaggc ttcttttaaa
    16261 ttatcgcaaa ggattcctga tgaattactt caagaaatcg tacaagcaaa caagctttat
    16321 cgtgaggatt tgtccgaacc ggctaaatca attgccgtaa tggaggattt gattgcaaaa
    16381 gatcgagtag acggaaatgc ttataatcga gaaagtctat tgcaaatatt tggtaagtat
    16441 aatattcagc tagattctga ttttgatatg gaaaaacttc aagaggaatt aaagtccata
    16501 aaagggcaac cgttagcagt ttatactata acaatgttgt ttaaacgtat gaagcttgga
    16561 tttgatcgtt ttaaaggtcg aaagcaacca aaagcaactg ctctgtttac aggatcaaca
    16621 ggggtcggta aaactgcaat ggctaaagcc gtggcaagag gactttatgg cgatgaaaat
    16681 aaagttctct tagttaatat gccggactac aaaacaaaag atgatgcaat gcgttttaga
    16741 cgactcatag gtactcatgt aaggcatgat ccttctgcga ttattgtgtg tgatgaatat
    16801 gagaaagctg gtgtagaaac actcgatgcc tttttatcaa ttttagatga aggtaggata
    16861 agttttactg aaagaggatg ggatgatcga gaagttcacg acactgtttc attgtcaaat
    16921 acaattttat ttgcgacaac aaatgcaggt gcggagatgt tcgattcgtt aaacagatat
    16981 agctcactat ctcgtgttac aaacaaggaa aattctaatg aatatagcac tgaactaaaa
    17041 agagaatacc gaaaagtcgc atcagggatc aaacaagcct tgattggtga aggtctaaag
    17101 cctgaagtta ttaaccgatt tggacgaata gttccatttt gggctttatc cgaacaaact
    17161 cttctggaaa ttgcttataa tgaaatgttg aagagtgctg aattagtacg aaaacattta
    17221 aatatagatt tacgtcttcc acctaggaaa gaaagagtat atggtcaaaa tttttatgca
    17281 gacgatctct ccatgcactt agtttttgac atggcttcac aagaggattc taaatcaggg
    17341 ggtgcaagga atatcttgca tttagttgaa tctgagtttg aagaagcgat tactgaagca
    17401 ataattactt atccaaacgc aaaaatattc gatgttaagt tgagtgaagc cggagcgttt
    17461 gaaggaaaag gtgattctaa tgctgaaatt attgcaaagc cttccgttgc tgaaagtaaa
    17521 gaaaacaaga ttgtattttc gattggaggg gcgtagctat gaaattcaag aaatatttac
    17581 tacaaaacct tagaataaaa cgatgggtcg taagaggtct agttattgga ggagtattag
    17641 cagttacttt aggcgctcct tttggaatga atgatccaac tcctgacaat caagatagca
    17701 aacaagaaac agttataaat aagaacgttg catatgcgga aagtaaaccg gcagaacaag
    17761 gaatgtggac actatatgct cgtatcctaa tgggagataa agaagttaat gatagtgccg
    17821 ctaaaactgg acaaatgaat ttgttaggac aaggtggtat cagtgccgat ttcccttatt
    17881 ctgacatttc taaacaaggt aattcgattg cagggggtaa tgcaggtact caactagcat
    17941 ctactttagc aacttatagt tactacggat atattgaaac agtctcagga aatgctattg
    18001 ccagtcaagc atcatccatt ctaggaagta ttggtaggac gattggtagt tttatcggat
    18061 caattagttt aatcctttct aatattgctg aatccataaa tgaactcctt ggaaaatttt
    18121 tcatgagtat gaatatattt tctcttatgg ggtggggaga aggtggatcg gatgtatcta
    18181 atccggtatc taaagcatta actagcttgc ttaacaattt gggattaaat aatgaattat
    18241 ttaaagcgat agcaagctta tctatgctct ttttcacagc aatactaggt tatcaaggaa
    18301 tgaaattatt gtcgaaagat ggtgttaact cgaagggatt atcaccagta cttgaaaaat
    18361 ggattgttcg tatttttgta ttgtttatat tcttaccagt ggcaggatcg ctattgggag
    18421 tttatatcaa cggcatccag tcagcaaaaa aagatggaga tataccaaca aatgttgttt
    18481 ctgactacat cattaacaca agactttggg cttctactca aaatcttgct ccatcatctg
    18541 atactggtgg tgatgtacct gacggaaagc ctacatccga aatcaagttc gttgatacag
    18601 attatgaacc atctaaaaaa tctgcaatga taagagcgat caatactagc agttatgcca
    18661 gacaaaataa tgatctagat tctaattcag tcggtatggc tctattaaaa gaatggtcat
    18721 cgaatgatac tttcaatatt aatacttatg ccggtgatgt taatgtcgcc gaaaatgatg
    18781 cttatatggg aagagcgcct gctgtttgga caggctataa tttaggggat ggaaaagaag
    18841 gaagtgcaat taagaaacca accaatttaa atggatatat ttggtcagca aatcaaaatg
    18901 tgacagacga caatcgcaag cctgatggaa aaggaacagc gcataacttt gatgctactc
    18961 aaccttgggg cgttgctaac aatgcaactt ttagcacaca gtcggtagct ttattgctcc
    19021 aatcggcact tgatacatcg ggagctaagt tttatgcgta taatatcgct ccttcaggaa
    19081 ttcaaggcag tgctaagaat atgtcaacag taaaaacaga atggcgtaca gcatccttag
    19141 ttggtcatgg agctatgggt aagtttggct cgtggctagc gatgaacgct gaatcaatcg
    19201 ttttagcagt tctttactta gcatgtgctt gggcgatgtt caatgtgaat atcgtgaaag
    19261 catttaaacg tgaatttatt cggatttggg aagtagttct aacaggatca cttgcatcag
    19321 gagcaggtgt gatggcaatt tcattaggtg ttgttacaac atctctgatc ggactacaat
    19381 tacctatctt tctgattact tttattcgtg catttggtga tactgctgtg ggattcattc
    19441 ctaatacatt agttggcgat ggcgtagcaa atattgttcg atctgctatg attttaggtg
    19501 taggatatct tttagtcttt aaaaaaggcg aaaaagtaaa taatttgatt aatatgctta
    19561 ttggcttacc acttgaagta gcgtttgggt ttgatgaacg tgctaagtta ttatttggta
    19621 atagaaatga tgctgtgcga gttgtggacg gcgtttctaa agcaatcaag aaaagccaag
    19681 gagctaaaaa tccctattat gatggtggca acagctcagt aaaagatatg gttgcaggag
    19741 gtcttggagc agttggagcg caaaaaatgg ctgaacgttt gaacaatatg ggagatagca
    19801 atgctgatgc taagtccatt gctgataaag taattcctga aacaccagaa ggaatgtcat
    19861 tcaacggtgg cggagttggt tccacaatca ataatacctc tttcaaagat ttagttcgtc
    19921 aagatcaact acgtaataac tcccaacaat atattgatca tttaggacgt atggttgatg
    19981 aaaacggaaa ctttgtcgat gaaaatggtg aaatattaaa agatagtaaa tacggtaaag
    20041 atgctaaagg caactttatt gacgaaaaag ggcgtttggt cgatgaagat ggaaacctta
    20101 tagatactaa tggtaaacca ttggaagata gtgaagatgg taatattggt atggatagcg
    20161 aaggacgttt aattgatgaa aaaggtcgct tagtcgatga agatggaaat cttgttgacg
    20221 aaaatggacg caggttgcta aatggtgatt ttggtcatga tagtaacgga cgtttagttg
    20281 acgaaaaagg tcgtttagtt gatgaagatg gaaatcttgt tgatgagttt ggtaataaac
    20341 ttggtaaaca cagcgatgga tataatgtga atggagattt agtcgatgag gatgggaata
    20401 tcattgagac gagagatgcg cttgtggcag atcgaaatag agaactcgaa caacaacggc
    20461 atacgccaat tgctgttgca attcctgaga atgaagaaga cgagattact ttagatcaaa
    20521 ctcatcaaga actgaggaat gaaatgagtg gtgaggacgt gtcagacaga gtgcatcggg
    20581 aattaggaga tggagcatct ggtgaaagtc agtcaagaga gcttaacagc aactttgata
    20641 atagtgctat tcggaatgac acagtactta aagacagaga atcgctaaca aaagaaacgg
    20701 ataaagaaca atcagatgtg aagaaaaagt tcattcctac tcctaatgtt aatcaaacag
    20761 ataaatatga aacaaatatt ggatcaggaa tgtcgaaaga caatcaatca attagagaac
    20821 ctgaactgac aaataaaaat aatagaacaa ataattctaa atctacttca gataattcag
    20881 ggaaatctaa tcaatcaacc aagaaaacat caacagctaa atcagccgtt gaatatctat
    20941 caaaaactgg taaaaatgtt tacacctcag cagataaagc tttagcaaaa gctccaaaag
    21001 gtagtctagc agggataacc tatgaagcta aaaacgaagc tattaaaata ggtaaaggaa
    21061 cggctaaggt tgccaaggct actgctacag gagcatccaa aactggtaaa gtagtgaaga
    21121 aatctaccat gtcagtcaag gactatattg taaaatacgg taatcaaaaa tcaactgaaa
    21181 ataagtctga agacgagtaa aaagaaggag cattgatttg ctccttcttt ttactttgat
    21241 ttctaactga agatgtcaag gaaaatttct atttatgcaa aaaaattatt ttaaattgtc
    21301 tataaatgaa ttaaatattt actgttcaaa gttgtataat taaaaagggc ttatgctggt
    21361 aaagaacaga attattatct aaacaaaaac ttgataaacc agcattttta gccaataaaa
    21421 aaagtgaaat aatttaatgg aaaatctctt ttaatcatcg aaaatttatg ttattatgat
    21481 aacataaaga tgtaaatatc gagacgtctt tttcttagtt agatcattga caagtgaaca
    21541 aataagattt gtcgatgata taattaactt attaaaaata ttaaaaagga ggtttttata
    21601 tgttcgattc tattaaaaat ttggttagcg gaatgacgcc tgatatgaaa actttagtac
    21661 aagttgtttt gtatttcttg gcattcgttt tttcgttgaa accaggattc actgcaatga
    21721 aattttttgg tgatgcaaaa tggggagatg gattaaaata tgttggggcg attattgcaa
    21781 tctttattat tcctgtaatt acaaccgtta tgctttacac aatgggtact aaaacaggta
    21841 atgatttgaa ccaaaaagct aatatcatat cagcacttat tccagttgtt ggtatctatc
    21901 tagtttcaaa atttccaaaa aatgaactgt taggttaaaa gtgaattgaa caaatttgga
    21961 taatatgccg atttgttcaa tttacttttt accaaaaaac tacgctccaa gcgtagttta
    22021 gaagtgaggg aagaaagtaa atgtaagatg gtttttgata gagaaaaaat gttagctcac
    22081 gccaacgaag tattaatgag cagtctgaaa ggtaccgaac tggcaaagat aatgaatatg
    22141 aatgttaacc agttctatga ttatcgaaat gggagtaaaa agattgaaaa agccagactt
    22201 gaaacgctta ttaaattcga aaaagcatat gtatatatgc tagataaaca gaaaagaact
    22261 attgactgaa aaaaggagtt ttacaataat ggcaaaattc aaagatatta ccgaatctat
    22321 taattatgtg gcagaaacag ttcctgtagt tgcactctct ttgaagccac aattactcga
    22381 agtgtacaaa aaagaagaaa aagaagcgaa ggcaaaaaaa gctaaagcaa aggtatggaa
    22441 agaattcgat gcgttgctga ccaaagattt accaaagaaa aattatatgg taattgaaga
    22501 tgtacaaaag ctcaacagtg agggagttat agatgaggat gatgctattc tcgattctga
    22561 gaagtatgtc gttctattga agtcaagttt gaattatgta gctgatctac cagaaaaatt
    22621 taaagagaag ctattagcag gtgtccgttt tatggatagt agtggtcaat tattagttat
    22681 tccggatgat cctaatgtgt ttgaatcaga agttgaagaa gctacagact tctatagcgc
    22741 agtagagcaa ttagaagtag ctactaatgg attagaacga attgctgaga ccttcaactc
    22801 ttctgaagaa gttgaatttg aagccgtagc agaaaatgta ccagatgaaa aagaagctct
    22861 ttttgtggat gataacaaac caaccgaagc agaaaaccaa gaagtaagca tagaagaaac
    22921 acaaatacaa acagaaacct accaacaacc aaaaattgat gaaaaacccg aatcccaaag
    22981 tgacaaagaa aattatgttt atgaagctga aaaaatcaca tctgaaagtg atgtaccgaa
    23041 aaacttagat gctgattttt tagaaaaaaa ttttgaacag atcattgctg acaaatttca
    23101 acctgtagtt ttagatgaga ttaatgaccc tgtcgattta aacgaggaga tgctatccat
    23161 acctgagttg aaaccattgc tgtttttagc taaagagagt atgaacgctc gcatctctat
    23221 tgctaataat aaaatcaaca gtgaattaaa taagacgaag gagcaattga tcctcaaagg
    23281 aaagcaaatg ttagctgatg agcttttgcg gatagaggaa gcaacctcat taaatggtca
    23341 aaatcagttt acaaatacac tatcggttgc taaagagagc tacgaagcag aagcatctaa
    23401 aattgatgat ttagtgatgg aaaaatccaa tatcattcgt aaagattatg atatggcgaa
    23461 acaggcttat ttggatgctg aactaaagaa actagagcaa cggtatgatg ctgagcattt
    23521 accagaagtt aaacaacaag aagatgaata tagaaaagca actgaagaaa gtcttgatca
    23581 acagtaccaa gaagcaattg aatctattaa aaacgatgca ttgaaacaaa gccaagaagc
    23641 aaaagaagtt gtggtggaac gaattatcaa ttcattgaaa ccagaaattg aaaaatcatt
    23701 aacagacgtg caagaaaaaa ctttagagat tgtcgaaaaa acaaagtcag aaaatgaagc
    23761 tgagttccat gatacaaaag agaaaacaac agatgtggtt gtcaaatatt ttgcgaacaa
    23821 agaagaagtt gaaaagcagg tagctaacaa cacgaaagaa ttagaaacaa aacttgtaga
    23881 gcaacaaaaa actatcgaag aatataaaga tcgaatgaaa gaggtcaaag atttagcaga
    23941 gggttataaa ttaaatagcg aaaagttggc agaacaaagt gaagctttaa gagaagactt
    24001 atctcgttcc cgaagtgatc tagctcaaat ggcagaaaaa acgaaatcat tagcagttgc
    24061 tacagaaaat caatcaagtt caaaacgtga ttttacaagt aaaaagatgg ctactatcat
    24121 ttctgtcgct gcaatggctt gcggaacgat cttgggatcg tcctacttta ttagtcataa
    24181 taatcaacaa gcacaggctc aaactcaaac atctatttcg agagtggaaa attcgattgg
    24241 gacaaatacg aactctgctt caacaaatag tagctatgaa gcaacaccaa ctacagagtt
    24301 caaagttggg aaagatgttg tcatcagtat tgatgggaaa acaactaagg ctgaagtgat
    24361 ttctattgat ggagaaaaag cagttgtgaa aactccagac ggaaatcagt acaaaattca
    24421 agctcactta tctgataata actaaggagg gctattatgc gagaagaaaa aatagtttat
    24481 ccagtaattc aaaacaatca agaaaacatg acctcttctc agcacttaca ggaagcacag
    24541 ctcgtgtatc aggtaaatga actaccacaa acgaacgaga caatgagcaa gtcaatatct
    24601 ttaattggct tgcttattgt tgcaatagtg ataactgtag gtgtttaccg taagttgaac
    24661 gtgaaaggaa gtatttctga tggcagataa ctcaaactat ggatttaaac ataacttaca
    24721 tcaaaaacct tctgaaacaa ttgtcagagt tgaatggctt gaaaagcatg tcaatagcac
    24781 ctattctgtt acagcaaaaa gattaattgt atcagctata gttgttgcag tcgttgttct
    24841 tggatcaatt gtttacttag tttctgatac gattaaactg aaatttattt ttgttccaac
    24901 gttattaatg ataattggat ttcttattct aattagccag tatatgttta ttgatccatc
    24961 tagtggttca ttgatttctg gttctattag actgttcaaa tcagtagtta attcagcaag
    25021 agtatctatg gggaaaaaga aaccatttcg aggcacagag attttaaaag tgaaacaaga
    25081 tggaacaatc gtatatcgag atgggagtgt agcattaatg ttcaatctag atggggctac
    25141 atcactaacc gcttttccag aagaagttat ggctcaggaa tcgaaatcaa tacaatatca
    25201 aaataccaga gatcgttcct tagctgaaat caaaatcact tcatctcaac ctcagaatac
    25261 ggaaagacaa actcgaaata tgaaaataca aaaacgaaat gcaaggagta acaaagcagt
    25321 ctttgacata atagatcagc aagaacgatt tgtatcagaa tatattgatg gtgtgaaaac
    25381 aacagtaatc caatatctta tcttgaaatc tcaatctaag atggtattgg atgaaggtgt
    25441 ggaacgttta gaaaactata cgagtagagg actttattat agtgtcactc agttgtcagc
    25501 taaacaaaca aaagaaatat tatctgatat ttatgcactg aaatagaggt gagagattaa
    25561 atggaaatta tgggtagatc acaactatta aatttcaaaa aaaagagaaa aactaaaaaa
    25621 gaacaaaaaa ctagaaattc aaaggtagtg aataatacct ttgttagtga tttaacatct
    25681 ttagttggtt atcaatttac accatcttac attaggacag gtaagcggtg gggaacaatt
    25741 gtgaaattag tgaatactta tggaatgaat agaaattatg gatttggttg gatggttaaa
    25801 gctattcctg aagtgagagt agaaggggta aaagcctatc taattgaagc agataagcca
    25861 gtgtcacaaa aagatcaaca agaaataatg cacgagaatg ttgggggatc aacgaatagt
    25921 tatgaaaata atttagaagg aaaaaattca gatgctgaaa ttagcaaaac tgttatgcgg
    25981 attatggatt tgaatacagc atctttacta gacagcatgg caaataaaat tattgattgg
    26041 aagctagaaa tcctattagt atccgattct cctaataaaa tcagcgaaca gctaattaaa
    26101 ctccaacggt tgtatgatga aaaaatgtat ggattaaagc ttatgtcata tggaggagat
    26161 caacgtgatc tgtttgatag acttttaagt gaaccaatcg ggaatgtcta tgagtatacg
    26221 tctatgagtt cggattttgc aggaaatgat catgcaattc gcaaaggttt agatgatgcg
    26281 aatggatggg ctatcggcac tatgtctact agctacgcaa gtggtgtagc ttttatggca
    26341 ctggatgatt cttttacaag taaagtttta gtggcatcac acaaaaacgc tacaattaaa
    26401 ggctatgatg aaaaaatctc agcatcatca ctttgggggc aattgattgc taataatgca
    26461 atggcaaatc ataaacgaac ttttcatatt gtcttaaata aatttgatta ctttggtgat
    26521 ccaactggta gaacatttgt tgccaaacct tcgttaaaca gtgtaataga aaaggttaat
    26581 ttggctgaag aaggtcttaa ctctttggag ctttttggta atcaaaagga cattggtgat
    26641 ttatatagac ggaatctcga taaactaact cagatttttt atttgttagc aggcagaaca
    26701 ttagattctg caactagagt tttattgaaa aaagaattag gtaactttta tataagtcag
    26761 aaactttggg atattaatta tgaagaacgt tcaaaacttt tggtgaacag tagtgataca
    26821 atgccagttc ttggggattt tattagctat ttgactaatg ctgaaacaaa agccaaaaaa
    26881 tctggtactg aaaaagaagt cgatcgtatt attgtattaa aagacacatt gagaacagca
    26941 ttagacagct tctctaggat gtttaatcaa agaacgacac tgaaagaaat cggtaaaagt
    27001 gacaaattac agttttacta taacttaggt gatctaagag cagaaaccaa tgttatggaa
    27061 gctcaatttt taaatacctt tgatttcatc acatcacaag caaaaaaaga tgatattgtt
    27121 atgattcacg gcttcgataa gttatctatt gaaaccgttg ggctaattaa aaaaagatta
    27181 acaatgcttg aagaagatgg aattcgtctt gcctttctat ttgatacaat cggttcagga
    27241 gaagacaatg aatacaaaga agatattgaa acagctgata tattcaatac agatggaaaa
    27301 atttaccaaa gttttgaaag tgattttaat tacactatca ttggaaagat gaccagtgga
    27361 gagttaatga agtatgaaaa aaaagttgct cctgctagaa aaggagaaga tgatcaaaaa
    27421 ctttctgttg atttgagaca gtatttgaca acaggaagaa gtaataaatt tcagatcagg
    27481 agaccagtgg atctgactaa taattttgtt attggagagt ttattcttta agttttatct
    27541 ttgattttga gtgtataagg aaaggataag aaaaaacatg tatatttgag actaaaaaca
    27601 gtcttagaga acatggtacg ttttgtgaaa agagaactat agaaatttca taaagtgaaa
    27661 ccttactgag tgaaataatt atagtggagg ttaaataatg aaaccaaaca taaaaatttt
    27721 agatcgaata tttctaggta gagacacaga agtaatccta attcaacatg aagaaggatt
    27781 tgaagttagt ataggtattc agaaacttca aaaacctcat tattgtaatc agttgtataa
    27841 aaactttact gatgaagaaa aagcgagagt ttt
//
LOCUS       DAXWLH010000018        27865 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXWLH010000018 DAXWLH010000000
VERSION     DAXWLH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.018"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(397..2289)
                     /locus_tag="ACGE36_000569"
     CDS             complement(397..2289)
                     /locus_tag="ACGE36_000569"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382992.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2286..4013)
                     /locus_tag="ACGE36_000570"
     CDS             complement(2286..4013)
                     /locus_tag="ACGE36_000570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8382993.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4029..4679)
                     /locus_tag="ACGE36_000571"
     CDS             complement(4029..4679)
                     /locus_tag="ACGE36_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFF8382994.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4883..5179
                     /locus_tag="ACGE36_000572"
     CDS             4883..5179
                     /locus_tag="ACGE36_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8382995.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5371..5991
                     /locus_tag="ACGE36_000573"
     CDS             5371..5991
                     /locus_tag="ACGE36_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8382996.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6083..7249)
                     /locus_tag="ACGE36_000574"
     CDS             complement(6083..7249)
                     /locus_tag="ACGE36_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8382997.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7261..7614)
                     /locus_tag="ACGE36_000575"
     CDS             complement(7261..7614)
                     /locus_tag="ACGE36_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8382998.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7742..8764
                     /locus_tag="ACGE36_000576"
     CDS             7742..8764
                     /locus_tag="ACGE36_000576"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFF8382999.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8783..8944
                     /locus_tag="ACGE36_000577"
     CDS             8783..8944
                     /locus_tag="ACGE36_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383000.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9203..9637)
                     /locus_tag="ACGE36_000578"
     CDS             complement(9203..9637)
                     /locus_tag="ACGE36_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383001.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9747..11606)
                     /locus_tag="ACGE36_000579"
     CDS             complement(9747..11606)
                     /locus_tag="ACGE36_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8383002.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMVLFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11607..11747
                     /locus_tag="ACGE36_000580"
     CDS             11607..11747
                     /locus_tag="ACGE36_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFF8383003.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11724..12883)
                     /locus_tag="ACGE36_000581"
                     /pseudo
     CDS             complement(11724..12883)
                     /locus_tag="ACGE36_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13190..14614)
                     /gene="araA"
                     /locus_tag="ACGE36_000582"
     CDS             complement(13190..14614)
                     /gene="araA"
                     /locus_tag="ACGE36_000582"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFF8383004.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14630..15328)
                     /locus_tag="ACGE36_000583"
     CDS             complement(14630..15328)
                     /locus_tag="ACGE36_000583"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8383005.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15285..16946)
                     /locus_tag="ACGE36_000584"
     CDS             complement(15285..16946)
                     /locus_tag="ACGE36_000584"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFF8383006.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17164..18564
                     /locus_tag="ACGE36_000585"
     CDS             17164..18564
                     /locus_tag="ACGE36_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFF8383007.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18816..19895)
                     /locus_tag="ACGE36_000586"
     CDS             complement(18816..19895)
                     /locus_tag="ACGE36_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8383008.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19981..20940)
                     /locus_tag="ACGE36_000587"
     CDS             complement(19981..20940)
                     /locus_tag="ACGE36_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFF8383009.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20952..22463)
                     /locus_tag="ACGE36_000588"
     CDS             complement(20952..22463)
                     /locus_tag="ACGE36_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFF8383010.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22485..23048)
                     /locus_tag="ACGE36_000589"
     CDS             complement(22485..23048)
                     /locus_tag="ACGE36_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8383011.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23054..23917)
                     /locus_tag="ACGE36_000590"
     CDS             complement(23054..23917)
                     /locus_tag="ACGE36_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383012.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23931..24827)
                     /locus_tag="ACGE36_000591"
     CDS             complement(23931..24827)
                     /locus_tag="ACGE36_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383013.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25002..26291)
                     /locus_tag="ACGE36_000592"
     CDS             complement(25002..26291)
                     /locus_tag="ACGE36_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8383014.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26263..27780)
                     /locus_tag="ACGE36_000593"
     CDS             complement(26263..27780)
                     /locus_tag="ACGE36_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFF8383015.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8138 a         5867 c         4829 g         9031 t
ORIGIN      
        1 agaaaaattt tgtgaaaatg gctttctaca tttttgcata aatttggctt aataccgaga
       61 aactggcttt tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctccgagtca
      121 caaggagcaa tttcaaaaaa cagcttctca ggtttgttga ctattgtgac ttgtgaccga
      181 ggagttggca ggagaacacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag
      241 tattagagat acaaaacgga aaaatagctt ctcatatttt gccgtttttt gagctaatac
      301 cgaaggacag ctgattgaac accgtttatt cggaaaaagg gactgtgaca cgacttttgt
      361 cacagcccct aaaaaaaata ataaatttgt actattctat gaagcttctt cttcagaaaa
      421 ctgactgtta taaagatctg catagaatcc gtctttttcc atcaatgtat cgtggtttcc
      481 tgtttctacg atcgaaccat gattcatcac aataatgttg tctgcatcgc gtattgtaga
      541 caagcggtga gcaacaacga aacttgtccg gttttccaat aggcggttca tcgctttttg
      601 aatcagtatt tctgttcgtg tatctacact agatgttgct tcatcaagga tcaggacatc
      661 tggatttgct aagaatgccc gcgcaattgt gattagctga cgctgccctt gcgaaatatt
      721 gcttgcatcc tcgtttaata cagtgttata tccttctggc aatttacgaa caaaatcgtc
      781 tacgtgagca gctttagcag cctcaatgat ttgttcgtct gttgcatcgt cgttcccata
      841 tttgatattg tcatagatac ttcctgtaaa cagccaagta tcttgcagta ccattgaaaa
      901 ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct acaccatcgt aacgaatact
      961 acctccagaa acatcgtaga accgttcaag aaggttgatc agtgtcgtct tccctgcacc
     1021 agtcggacct acaatagcga ccatctcacc aggtttgaca tccaagttaa aatcagtcat
     1081 tagcaagtta tctggtgtgt agccgaattg aacattttca aaacgaactt tgtatggtgt
     1141 atctttttct tctggaaggc cagatttttc attggacatc tcttcttcat ccaacacttc
     1201 aaatacccgt tcggctgaag caactgttga ttggatagtg ttcatcaaac ttgcaagttg
     1261 agtgattggt tgagagaact gtgttgtata ttgcaagaac gcttgaacat cccctaatgt
     1321 catgttccca tttgcaactt ttactccgcc tagtacagcg acgaacacat agcctagatt
     1381 tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata aattgagctt tccaaccagc
     1441 ggcatacaga ttctgatttt ctttttcaaa tacttcctgg tctttttcag tatgattgaa
     1501 tgttttaaca atggtatgac caccatatgt ttcttctact tggttattca ataaacctaa
     1561 acttttttgc tgatctgcga aaaacacttg tgatttcggc gctacgatca tcacgatgat
     1621 caagcttaaa gggatcatca ataacgcgac taacgtcaat tgccagctga ttgaaagcat
     1681 catcacgatg accccaatca atgtgactgc actagttaca aattgagtca aattttgctg
     1741 cagcgtgctt gcgatgttat ccatatcatt gattgctcga gacatgatat ccccatttgt
     1801 atgtgtatca taataggaaa tcggtacacg attcattttg gcttccaaat catgacgtaa
     1861 ttcataaact gttcgttggg aaacacgtgt cataataaat tgctgtaaaa agttgaatac
     1921 agcagaaatc aaatacatca cgattactgt cgcaatgatc tgcgcgattt tatcaaagtc
     1981 tatcggaaat tttgttactt gttggccttg ttgcatctgt tgagcgccag ccattacacc
     2041 tttgaatatt tctgtggttg ctttccccaa gacctttggt gtttggattt ggaagatcac
     2101 ggctgctatt gctagtacaa atacagcgat gatcgcaacc atacgtttag acatatagcc
     2161 aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt tcacctttta caccgagatt
     2221 tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca tgagagggac gtgtgtttgt
     2281 tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa cgatttcttg ataagtttcg
     2341 tttgttgcta atagttcttc atgtgttcct ttgccaacca gttttccttc atctagcaca
     2401 aggatcatat ctgcatccat tactgtactt actcgttgag caactaaaac ggtgatactt
     2461 tctttcatat tcgttttcaa tgcttggcga agattggcat ccgttttgaa atccaacgct
     2521 gagaaggaat catcaaacac atagatatct gctgttttga ctaatgcccg tgcaattgcc
     2581 agacgctgtc tttgtccacc agagaagttt cctcctcctt gttccacatg gctatctaag
     2641 ccatcttcca tctctgaaac aaaatcttta gcttgtgcga tttctaatgc tttccaaatc
     2701 atttcatctg ttgcatccgg cttaccatac tgcatattgt ctcggatcgt tccagagaac
     2761 agaactgctt tttgaggaac aaagcccatt aattctcgca aattatattg agtcatgtca
     2821 ctaatgtccg ttccatttat ttggatcgaa ccagattcga tgtcatataa tcgagggatc
     2881 aaattgacta aggtcgtttt acctgaacca gtccccccta tgattgcgac gatctcgccc
     2941 gacttggctt ggaaatcaat atcttccaat gcaagttttt ctgctccatg atatcgataa
     3001 ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa aagaaactgt tttaggattt
     3061 tcagggtcat ggataccgat tttttcatcc aacacttcat taatacggtc agctgaagct
     3121 tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca taaagctgat caagatctgc
     3181 atcgcataag tcataaatgc aatcagatta ccgacttcca aagtcatatc tgctatgtag
     3241 tgtccaccaa accaagtgat tgctacgttt gtaccgctca tgatcaatgt catcattggc
     3301 atcatcaatg cgacgatcgt attaactttg attgccgtat ttgtataatc tttattcgct
     3361 aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg ctcgaatcac tctgacccct
     3421 gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt tcttttgcat acttttgaat
     3481 aatggaacag caaagtacat gattccgccg atcaaaataa tcattactgg gatgacaact
     3541 aagaagattt ttgtcaactg atgatctttt tgataagcaa gtatgctcgc gccaatcaac
     3601 gtgattggtg cattgatcat catacgtaaa aacatttgtg taaccatttg gatttgagtc
     3661 acatcattgg tcgttcttgt gatcaaagag gccgttccat atttatcaaa cgcatctttt
     3721 gtcagactct ctgatttttt atagacatcc gtacgcaatt gcttacccag tttctgtgat
     3781 tcacgagtag caaaaaatgt atttgcgata gcagctaaga tactgatcaa agaaaagccg
     3841 atcatcacaa aacctgtatg ccaaatataa tcgacatctc cttgcgctac ccctttatca
     3901 ataatattgg aggtaagtgt cggcaaatat aaatctgcca gtacttggat caccatgaaa
     3961 actgcagctg caattgctga agtcaaggag atccgtttta cgatttttag catctagttc
     4021 ccttctttct atttatatac tccgtttttc agccaagaaa gtttcaaatt gaattcactg
     4081 attgcttgct ctactgagta atcttcagat gttgaaaata agcgatacga atgcgcaatt
     4141 gttgtaaata cagtattcat cactaattgg atcagtaatt ccaattcatg atcatttttt
     4201 actttcagct tcgtccgatc gatcaattca agcaactctt ttgcatcttg tttgtgttct
     4261 ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag gagcgacttt tcttgaggca
     4321 cggtaatcca tgttcataaa caagttacga taaaaagaag cattctcgcc ttccaaaatc
     4381 tcataaatca ttttagaaaa ataggattcg aatcctttga atagatcacc atcggattct
     4441 ttcattagtt gtaacatatc tttagagctg tcacgtctca ttgtctcaaa gtaataaaaa
     4501 taaagatcct ctttatcttc aaaatactga tagaaactgc ctctagggat atccgctaat
     4561 ttgacgatat tagcaatcga agcttctttt aagggtactc tagaaaactc gattctcgca
     4621 gcctcgatta gtcttttttg tttttcttta ggcaaatgga aaaatgtctg tgtcggcata
     4681 tggattctcc tttctttcaa gatgacacat tgtcatatga cgctatgtca ttataacacc
     4741 tcctacctgt tttgcaagac atttgcatag tttttttcat tatcatccga tacattaaaa
     4801 aagtgataag aggaatcctt ccatttgaat taatgagagt tgcccattaa aatagccaca
     4861 tactaaacaa aggatgagat aaatggccca atcaattagt gtccaagact tcaataaatt
     4921 gcctctcgat gctgacagca tcgttttaga tacaagaaat accttagatt acacaaaaaa
     4981 ccacttaagc cactcgcttt ctatccccgc tgctatcttg ccaagacaat taaaaaatct
     5041 agatccaaag aaactttatt atattctaag ctatacaggc aggcgatcag aagcattagc
     5101 cgatcaactt actgctaatg gttttcgtgc tgtttatatc attggtggga tggaagctct
     5161 tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa agaggctgtg acaaaaaatc
     5221 gtgtcacagc ctcttttata ccctgtcttt ttttagttat ttattcttca accttatcaa
     5281 tatgacttga tagccttttc tttttcgagt aaactgattt tgttagtcat agttataaca
     5341 atcatagcaa taaaaggaga aggtaagcat atggcatctg ttttagtagt caaaggtcat
     5401 cccctaactg cagaagaatc gcgcacagtt aaggcattga cgtctttttt aacaagttat
     5461 aaagaaaatc atccagatga tgaagtgact gttttggaac tttatcgcga tgatattccg
     5521 gaaatcgatg aagaattatt atcaggttgg gaagcattac gtgctggtgc agaattcact
     5581 tccttatcag aaagtcaaca acaaaaaatt gctcgtttca atgaattgac tgatcagttc
     5641 cttgctgctg ataaaatcgt tatcgcaaac gccttatgga atctaaatat cccaacaaaa
     5701 ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa cgttccgcta tacagaaaac
     5761 gggccagaag gattagtgac aggtaaaaaa gcactgcaca tccaatctaa tggcggtgta
     5821 tacaacggac aagactttgc ctctcaatat gtaaaaggta ttttaaactt tgtaggcatc
     5881 gatcaagtag atcagctatt catcgaagga atcgattatg accctgaccg tgcagatgag
     5941 ttgatgcaaa atgcattaga taaagctgct gctttaggta aatcatttta aaaattgaga
     6001 taaatgcata caaaagagac agaggaaaaa atcttcaaag cagcatttgt gccttggata
     6061 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
     6121 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
     6181 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
     6241 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
     6301 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
     6361 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
     6421 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
     6481 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
     6541 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
     6601 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
     6661 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
     6721 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
     6781 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
     6841 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
     6901 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
     6961 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
     7021 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     7081 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     7141 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     7201 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     7261 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     7321 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     7381 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     7441 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     7501 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     7561 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     7621 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     7681 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     7741 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     7801 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     7861 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     7921 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     7981 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     8041 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     8101 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     8161 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     8221 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     8281 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     8341 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     8401 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     8461 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     8521 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     8581 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     8641 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     8701 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     8761 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     8821 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     8881 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     8941 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     9001 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta
     9061 tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa
     9121 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
     9181 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
     9241 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
     9301 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
     9361 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
     9421 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
     9481 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
     9541 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
     9601 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
     9661 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
     9721 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
     9781 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
     9841 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
     9901 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
     9961 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
    10021 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
    10081 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
    10141 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
    10201 gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca
    10261 tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc
    10321 tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact
    10381 tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc
    10441 atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta
    10501 tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct
    10561 gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt
    10621 tgcgcattaa ataacaccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt
    10681 ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa
    10741 taatctgcca atggctcacc agatacttct acgccgttta atcgactagg atctgagaaa
    10801 ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca
    10861 tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca
    10921 ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta
    10981 tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc
    11041 aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc
    11101 agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac
    11161 cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg
    11221 ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc
    11281 ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg
    11341 acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata
    11401 atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc
    11461 aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca
    11521 acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc
    11581 gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat
    11641 gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa
    11701 tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt
    11761 cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta
    11821 ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg
    11881 atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt
    11941 tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg
    12001 accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt
    12061 ccgctgtctg ccaaagcttc ctgagtcgat cccctagatg aggcaacata agtatacggt
    12121 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
    12181 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
    12241 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
    12301 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
    12361 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
    12421 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
    12481 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
    12541 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
    12601 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
    12661 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
    12721 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
    12781 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
    12841 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
    12901 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
    12961 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
    13021 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
    13081 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
    13141 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
    13201 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
    13261 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
    13321 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
    13381 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
    13441 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
    13501 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
    13561 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
    13621 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
    13681 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
    13741 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
    13801 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
    13861 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
    13921 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
    13981 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
    14041 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
    14101 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
    14161 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
    14221 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
    14281 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
    14341 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
    14401 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
    14461 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
    14521 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
    14581 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
    14641 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
    14701 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
    14761 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
    14821 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
    14881 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
    14941 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
    15001 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
    15061 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
    15121 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
    15181 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
    15241 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
    15301 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
    15361 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
    15421 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
    15481 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
    15541 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
    15601 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
    15661 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
    15721 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
    15781 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
    15841 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
    15901 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
    15961 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
    16021 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
    16081 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac actgttcgta
    16141 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
    16201 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
    16261 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
    16321 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
    16381 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
    16441 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
    16501 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
    16561 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
    16621 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
    16681 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
    16741 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
    16801 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
    16861 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
    16921 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
    16981 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
    17041 aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
    17101 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
    17161 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
    17221 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
    17281 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
    17341 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
    17401 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
    17461 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
    17521 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
    17581 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
    17641 tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
    17701 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
    17761 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
    17821 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
    17881 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
    17941 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
    18001 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
    18061 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
    18121 actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc agctgtttta
    18181 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
    18241 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg cgaaatattc
    18301 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
    18361 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
    18421 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
    18481 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
    18541 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
    18601 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    18661 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
    18721 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
    18781 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
    18841 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
    18901 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
    18961 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
    19021 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    19081 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    19141 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    19201 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    19261 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    19321 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    19381 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    19441 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    19501 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    19561 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    19621 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    19681 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    19741 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    19801 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    19861 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    19921 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    19981 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    20041 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    20101 tggtcgagca tgatagatca aagtatcaaa gcacccgtct tctgaaaccg taaacgagtt
    20161 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    20221 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    20281 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    20341 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    20401 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    20461 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    20521 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    20581 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    20641 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    20701 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    20761 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    20821 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    20881 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    20941 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    21001 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    21061 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    21121 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    21181 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    21241 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    21301 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    21361 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    21421 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    21481 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    21541 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    21601 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    21661 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    21721 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    21781 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    21841 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    21901 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    21961 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    22021 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    22081 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    22141 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    22201 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    22261 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    22321 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    22381 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    22441 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    22501 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    22561 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    22621 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    22681 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    22741 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    22801 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    22861 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    22921 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    22981 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt agtctcaact
    23041 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    23101 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    23161 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    23221 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    23281 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    23341 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    23401 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    23461 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    23521 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    23581 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    23641 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    23701 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    23761 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    23821 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    23881 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    23941 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    24001 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    24061 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    24121 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    24181 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    24241 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    24301 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    24361 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    24421 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    24481 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    24541 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    24601 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    24661 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    24721 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    24781 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    24841 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    24901 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    24961 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    25021 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    25081 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    25141 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    25201 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    25261 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    25321 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    25381 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    25441 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    25501 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    25561 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    25621 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    25681 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    25741 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    25801 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    25861 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    25921 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    25981 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    26041 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    26101 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    26161 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    26221 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    26281 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    26341 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    26401 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    26461 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    26521 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    26581 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    26641 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    26701 tgtttctgtc ctgattggtg caataacatt taccaattga gcaagacaag caatctttac
    26761 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    26821 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    26881 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    26941 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    27001 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    27061 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    27121 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    27181 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    27241 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    27301 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    27361 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    27421 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    27481 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    27541 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    27601 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    27661 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    27721 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    27781 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat
    27841 ataaatgtta aacttactta ttttt
//
LOCUS       DAXWLH010000019        27754 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXWLH010000019 DAXWLH010000000
VERSION     DAXWLH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.019"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            150..1409
                     /gene="lysA"
                     /locus_tag="ACGE36_000594"
     CDS             150..1409
                     /gene="lysA"
                     /locus_tag="ACGE36_000594"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFF8383016.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1396..2196
                     /gene="dapF"
                     /locus_tag="ACGE36_000595"
     CDS             1396..2196
                     /gene="dapF"
                     /locus_tag="ACGE36_000595"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFF8383017.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2199..3080
                     /gene="dapA"
                     /locus_tag="ACGE36_000596"
     CDS             2199..3080
                     /gene="dapA"
                     /locus_tag="ACGE36_000596"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFF8383018.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3077..3793
                     /gene="dapB"
                     /locus_tag="ACGE36_000597"
     CDS             3077..3793
                     /gene="dapB"
                     /locus_tag="ACGE36_000597"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFF8383019.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3799..4494
                     /gene="dapD"
                     /locus_tag="ACGE36_000598"
     CDS             3799..4494
                     /gene="dapD"
                     /locus_tag="ACGE36_000598"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFF8383020.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4510..5511
                     /locus_tag="ACGE36_000599"
     CDS             4510..5511
                     /locus_tag="ACGE36_000599"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFF8383021.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5508..6869
                     /locus_tag="ACGE36_000600"
     CDS             5508..6869
                     /locus_tag="ACGE36_000600"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFF8383022.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6829..7980
                     /locus_tag="ACGE36_000601"
     CDS             6829..7980
                     /locus_tag="ACGE36_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8383023.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7955..9124
                     /locus_tag="ACGE36_000602"
     CDS             7955..9124
                     /locus_tag="ACGE36_000602"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8383024.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9299..10678
                     /locus_tag="ACGE36_000603"
     CDS             9299..10678
                     /locus_tag="ACGE36_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8383025.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10959..11756
                     /locus_tag="ACGE36_000604"
                     /pseudo
     CDS             10959..11756
                     /locus_tag="ACGE36_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11945..12613
                     /locus_tag="ACGE36_000605"
     CDS             11945..12613
                     /locus_tag="ACGE36_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFF8383026.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12813..13199)
                     /locus_tag="ACGE36_000606"
     CDS             complement(12813..13199)
                     /locus_tag="ACGE36_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383027.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13171..14553)
                     /locus_tag="ACGE36_000607"
     CDS             complement(13171..14553)
                     /locus_tag="ACGE36_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8383028.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14709..15896)
                     /locus_tag="ACGE36_000608"
     CDS             complement(14709..15896)
                     /locus_tag="ACGE36_000608"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFF8383029.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15903..16934)
                     /locus_tag="ACGE36_000609"
     CDS             complement(15903..16934)
                     /locus_tag="ACGE36_000609"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8383030.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16950..18404)
                     /locus_tag="ACGE36_000610"
     CDS             complement(16950..18404)
                     /locus_tag="ACGE36_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFF8383031.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            18579..19484
                     /locus_tag="ACGE36_000611"
     CDS             18579..19484
                     /locus_tag="ACGE36_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFF8383032.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19575..20252
                     /locus_tag="ACGE36_000612"
     CDS             19575..20252
                     /locus_tag="ACGE36_000612"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8383033.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20249..20419
                     /locus_tag="ACGE36_000613"
     CDS             20249..20419
                     /locus_tag="ACGE36_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383034.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20533..21429
                     /locus_tag="ACGE36_000614"
     CDS             20533..21429
                     /locus_tag="ACGE36_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8383035.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21484..22854)
                     /locus_tag="ACGE36_000615"
     CDS             complement(21484..22854)
                     /locus_tag="ACGE36_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8383036.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23092..23838
                     /locus_tag="ACGE36_000616"
     CDS             23092..23838
                     /locus_tag="ACGE36_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8383037.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23835..24674
                     /locus_tag="ACGE36_000617"
     CDS             23835..24674
                     /locus_tag="ACGE36_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8383038.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24675..25625
                     /locus_tag="ACGE36_000618"
     CDS             24675..25625
                     /locus_tag="ACGE36_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8383039.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25766..26518
                     /locus_tag="ACGE36_000619"
     CDS             25766..26518
                     /locus_tag="ACGE36_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383040.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26537..27688
                     /gene="queG"
                     /locus_tag="ACGE36_000620"
     CDS             26537..27688
                     /gene="queG"
                     /locus_tag="ACGE36_000620"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFF8383041.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9115 a         5045 c         5826 g         7768 t
ORIGIN      
        1 accattttta ttcttatttt taaaaaaata tttcgaaata tactagacaa ttcagaaaat
       61 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
      121 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      181 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      241 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      301 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      361 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      421 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      481 aaaatgattt gttacatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      541 taagccaacg ctatttaaaa aaaatttgta tcatgcttcg gttgaatgtc ggagtagaag
      601 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      661 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      721 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      781 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      841 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      901 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      961 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
     1021 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1081 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1141 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1201 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1261 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1321 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1381 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1441 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1501 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1561 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1621 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1681 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1741 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1801 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1861 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1921 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1981 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     2041 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2101 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2161 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2221 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2281 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2341 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2401 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2461 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2521 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2581 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2641 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2701 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2761 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2821 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2881 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2941 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     3001 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3061 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3121 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3181 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3241 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3301 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3361 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3421 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3481 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3541 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3601 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3661 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3721 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3781 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3841 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3901 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3961 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     4021 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4081 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4141 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4201 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4261 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4321 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4381 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4441 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4501 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4561 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4621 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4681 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4741 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4801 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4861 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4921 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4981 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     5041 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5101 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5161 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5221 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5281 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5341 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5401 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5461 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5521 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5581 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5641 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5701 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5761 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5821 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5881 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5941 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     6001 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6061 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6121 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6181 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6241 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6301 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6361 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6421 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6481 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6541 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6601 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6661 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6721 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6781 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6841 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6901 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6961 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     7021 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7081 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7141 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7201 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7261 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7321 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7381 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7441 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7501 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7561 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7621 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7681 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7741 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7801 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7861 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7921 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7981 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     8041 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8101 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8161 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8221 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8281 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8341 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8401 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8461 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8521 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8581 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8641 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8701 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8761 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8821 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8881 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8941 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     9001 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9061 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9121 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9181 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9241 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9301 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9361 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9421 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9481 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9541 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9601 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9661 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9721 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9781 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9841 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9901 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9961 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
    10021 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10081 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10141 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10201 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10261 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10321 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10381 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10441 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10501 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10561 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10621 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10681 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10741 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10801 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10861 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10921 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10981 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    11041 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11101 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11161 gtcgctacaa ttcttatcga ggaaaagttg gagcagtagc caaaaatcgg atccatcgtc
    11221 gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa tttaaatacc
    11281 atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat ccttttatgg
    11341 atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat gcattagcgg
    11401 taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat cgtcgtacgt
    11461 ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa ttgaaggagt
    11521 acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca cttatggaga
    11581 atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac tgtttcgagg
    11641 aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg ataaaacaga
    11701 aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt gtgtaaaaat
    11761 atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag tctaactttt
    11821 gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc ttccttgtat
    11881 attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt tagggggaga
    11941 aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt tattattagt
    12001 ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt tagtctatac
    12061 gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc atgtagggca
    12121 tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac gtatcgtcca
    12181 aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa tcaaagacgg
    12241 cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt tgcgagcaat
    12301 cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc tagagaatgc
    12361 cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga tcctgcttgt
    12421 agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg aagcatggct
    12481 tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt atgttgaatg
    12541 gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg aatcttaccg
    12601 aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt actcaaaact
    12661 aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt tgcttcttcg
    12721 aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat cctttcttat
    12781 tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt ttttgtttcc
    12841 tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat cataaagtaa
    12901 tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct tccaaattca
    12961 tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc tgtcagtata
    13021 ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg tgcttcttct
    13081 tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc ccattttaat
    13141 ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc
    13201 ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag aactacgtgc
    13261 gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa agccgtattt
    13321 ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct tttcaggatc
    13381 ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt tcacgcctac
    13441 acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag catctgctag
    13501 atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg tcttgccatt
    13561 tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg tttttgctgc
    13621 agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga tctcgatttc
    13681 ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt ttgattttgg
    13741 tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat aaagctgagt
    13801 accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta aatcataaac
    13861 agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc
    13921 agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc attcagcaaa
    13981 catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta gcgggttatc
    14041 gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat ggatcagttt
    14101 agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta catcaaggaa
    14161 gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt tatagataga
    14221 attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa tatataaatc
    14281 acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc cgtaagcttt
    14341 ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga
    14401 cggataggac atgactacta atttagttgt aaggggatca agatcctttt caacgcactc
    14461 tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag gttctttttc
    14521 ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa cacaattaga
    14581 acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga gcaaaaggct
    14641 cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg agatcgttga
    14701 tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt tccaatgtgt
    14761 gcaacataaa attccggagc atagccgaca actttcatgt attcatcata gacattgttt
    14821 ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc aaatccagct
    14881 cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac tagtgtatct
    14941 aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc atttaacagt
    15001 tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa agtccgctga
    15061 ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat ttggtcaata
    15121 gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa agttttttgc
    15181 aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga atctgccaat
    15241 tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt cactggaacc
    15301 atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc accgaagccg
    15361 atggcaaact gatccatgat acctgaattg acaccgatga attcattttc tactttttta
    15421 cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc taatacaaca
    15481 cctactaata attctaatga agcgctactg gaaagtcctg caccattagg gattgttcct
    15541 tccactaata actcaaaccc atgatcgatt gagtgtccag cttcttttaa tttaaggacc
    15601 attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc atctaaagta
    15661 aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt attatcgtct
    15721 cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac atggcctcca
    15781 ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt
    15841 tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg
    15901 agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac tgatccggct
    15961 tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt ttgagtctca
    16021 aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt ttctgtgccg
    16081 aaatttgcag tgaaaataac cacactagaa gcatctgtag caatatctac ttggattctt
    16141 ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt taagaaaaat
    16201 ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa aacggacgct
    16261 aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt tacccctata
    16321 ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag ctcatgttga
    16381 tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt cggattgaat
    16441 aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc ttttgtcaaa
    16501 gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc gggacttgta
    16561 gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga ccattttttt
    16621 aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc tgtggctaat
    16681 tggtaagttt ttccattgag agaaaagtgg ccattttcga ttctccctgc ggtgcgaccg
    16741 atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc aaatcccaaa
    16801 actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc agagatagaa
    16861 agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc aaaatctttt
    16921 tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc atcgtgaagt
    16981 tcatcttcca ttgtatcagc tggagccaat ttgttttcca atttctcaac gatttgtgca
    17041 tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa
    17101 gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc agtgatatca
    17161 gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac gatcccgtta
    17221 gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac agcttcgtta
    17281 cggataccat ttttccattg accatattca acagagtctg tgatcgtcat caaagcggat
    17341 aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac gattggcaaa
    17401 cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg
    17461 taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac agctactaaa
    17521 ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc aagtacatat
    17581 ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata agcgatataa
    17641 gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac atctttgatc
    17701 ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc caaggcagtg
    17761 ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa ccagcctgaa
    17821 gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat agggacaacg
    17881 actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt agcaaatttt
    17941 tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc gatatcctta
    18001 aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat aaataaagtc
    18061 gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag tacggaacta
    18121 acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg agtattatcg
    18181 atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc tacaactaag
    18241 cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc gaacatctgg
    18301 cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa
    18361 gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg tattcctctc
    18421 ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat cttttaatga
    18481 cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt tatttaactt
    18541 tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg atttttttaa
    18601 aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt ttctctaaaa
    18661 cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga
    18721 tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc
    18781 tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa gatccgtgga
    18841 tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt
    18901 tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc gacattattt
    18961 tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta acagcattaa
    19021 cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa
    19081 aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat tatgctgaaa
    19141 aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag
    19201 tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta cggatcaatc
    19261 gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc tatcaagtag
    19321 ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta
    19381 cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg gataccttga
    19441 aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc tggttaaaat
    19501 gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc gaagactaaa
    19561 aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc gaagaatctt
    19621 taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg catcatttgg
    19681 aatcaagtta tccagacgga aaaatagata tagagactga actacagctc ttggcccagc
    19741 atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct catttaaaaa
    19801 tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa gaactaggaa
    19861 tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa gaagaatata
    19921 cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat atgacctatc
    19981 ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt tgggagaagc
    20041 taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact cagcggattg
    20101 actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac
    20161 aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac ttgaccttta
    20221 cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc aacaattaga
    20281 tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact
    20341 gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg gacaatcttg
    20401 gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg tgcacagcga
    20461 caattatttg ctacaataag aatatgaagc gtttgataac gctattatac ataaaaattg
    20521 gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat gacccgaaga
    20581 agacagcaaa aacaagcagc tcgtaagcgg atgatcatga ttggggcagc agttgccgtg
    20641 tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa
    20701 gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt
    20761 gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc aaatggtttg
    20821 ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct
    20881 gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat agctggttta
    20941 tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga agggatcaga
    21001 aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt agatactttg
    21061 atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga tatttatttg
    21121 cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac atcttctgat
    21181 tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac acaagacaca
    21241 acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac aaccgtagtt
    21301 ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc
    21361 ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga gacgctaaaa
    21421 ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag cttcgttctt
    21481 tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa
    21541 aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga aaatcaatac
    21601 gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca ttttaaaagg
    21661 atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga tcaaaatcac
    21721 tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga aaatattttc
    21781 tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta agattccacc
    21841 tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca gaaatttttt
    21901 agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga aaatccctga
    21961 attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt
    22021 aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca attgattcca
    22081 cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga ttcgaccgat
    22141 agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac cagcagtcat
    22201 tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa agaaaaatcc
    22261 tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc ctactgcctg
    22321 accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca tcaaaatgat
    22381 cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat
    22441 aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg ccggcaaatc
    22501 aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc ctacgacaat
    22561 atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga tgtactcgct
    22621 cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg
    22681 cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg agggccctgt
    22741 ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc cacctagtgc
    22801 gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt ccatacaaaa
    22861 actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca catgtttttc
    22921 attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt tcataaaatg
    22981 cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt ccattttagt
    23041 taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga tttggaacgt
    23101 acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt tttgaaaact
    23161 atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa cggtgcagga
    23221 aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg taaagtcacc
    23281 ttttctggac agtcggtaaa ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt
    23341 gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg aacatatcca
    23401 tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag acaagctttg
    23461 aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc gggtggacag
    23521 cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat ctttttagat
    23581 gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa
    23641 ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa agtagaagaa
    23701 tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc agtacaagaa
    23761 gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca tttagtaaaa
    23821 ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat caattcctgc
    23881 aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc ggttctttta
    23941 ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt cttccaggtg
    24001 tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct tttgggatgc
    24061 taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat gatacagcga
    24121 ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca tatgcccaga
    24181 gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg gaaagcgatt
    24241 tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc ttttataaag
    24301 aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg aacacatcgc
    24361 tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt ttacagacag
    24421 taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct tacttgctaa
    24481 cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta agtgcagttg
    24541 tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc gtgttagcat
    24601 gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt ttatttcaga
    24661 aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca gttagtttct
    24721 tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa
    24781 agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag gtcggaacag
    24841 atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa tatgaagtac
    24901 taccagaaga catcaaaaag gcaagcgatg cagatgttat tttatacaac ggtttgaatc
    24961 ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa
    25021 aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc ggtgaagaac
    25081 atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa tatgtggagg
    25141 aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg
    25201 cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa agttttgctt
    25261 ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag tatttttcac
    25321 gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt caaggtacgc
    25381 ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg gttttattcg
    25441 tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa
    25501 tctatgataa actgttcact gattccatag caaaagaagg agaacaagga gattcttact
    25561 atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa acaaaagaaa
    25621 gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata agagatatga
    25681 aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt ccgttatagt
    25741 tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat atacgattaa
    25801 agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc aagaccttca
    25861 atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga caacagaacc
    25921 agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa
    25981 taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg gcgtattagt
    26041 taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag tcatcaaagc
    26101 agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta ttgccaacag
    26161 caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg ggaaaatccc
    26221 gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt attcccgcga
    26281 tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa gtacaaaacc
    26341 agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt caacaaatta
    26401 ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg ccgatcagca
    26461 gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca gccgttaaca
    26521 gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca aacgattagg
    26581 aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag aatcgttgga
    26641 agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg atgagcgtct
    26701 ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac ttgcgtatcc
    26761 tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc
    26821 ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga tcgaattcat
    26881 ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga
    26941 tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat ttattggacg
    27001 aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg agatcgttac
    27061 taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact gtatgcgctg
    27121 cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg cccagcgatg
    27181 tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta aaaaaatgcg
    27241 aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc aaggaaaaga
    27301 ttttcatttt catgaagaga tggaaccaaa agtagacgaa atatatccaa aattaaagcc
    27361 aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag ctggttcatg
    27421 gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc taggtgatcg
    27481 aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca tcagaggaac
    27541 agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt
    27601 agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg aacatgccca
    27661 gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa agtttttgtc
    27721 cagtaattgt tgtaacaatt actggttttt ttgt
//
LOCUS       DAXWLH010000020        27028 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXWLH010000020 DAXWLH010000000
VERSION     DAXWLH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.020"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(87..431)
                     /locus_tag="ACGE36_000621"
     CDS             complement(87..431)
                     /locus_tag="ACGE36_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFF8383042.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(568..1713)
                     /locus_tag="ACGE36_000622"
     CDS             complement(568..1713)
                     /locus_tag="ACGE36_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8383043.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(1893..2867)
                     /locus_tag="ACGE36_000623"
     CDS             complement(1893..2867)
                     /locus_tag="ACGE36_000623"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8383044.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2947..3822)
                     /locus_tag="ACGE36_000624"
     CDS             complement(2947..3822)
                     /locus_tag="ACGE36_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFF8383045.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3952..5859
                     /locus_tag="ACGE36_000625"
     CDS             3952..5859
                     /locus_tag="ACGE36_000625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFF8383046.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6400..9678)
                     /locus_tag="ACGE36_000626"
     CDS             complement(6400..9678)
                     /locus_tag="ACGE36_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFF8383047.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(9949..10494)
                     /locus_tag="ACGE36_000627"
     CDS             complement(9949..10494)
                     /locus_tag="ACGE36_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8383048.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10506..11198)
                     /locus_tag="ACGE36_000628"
     CDS             complement(10506..11198)
                     /locus_tag="ACGE36_000628"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFF8383049.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11217..11501)
                     /gene="macP"
                     /locus_tag="ACGE36_000629"
     CDS             complement(11217..11501)
                     /gene="macP"
                     /locus_tag="ACGE36_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFF8383050.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11514..12077)
                     /locus_tag="ACGE36_000630"
     CDS             complement(11514..12077)
                     /locus_tag="ACGE36_000630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8383051.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12305..12967
                     /locus_tag="ACGE36_000631"
     CDS             12305..12967
                     /locus_tag="ACGE36_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFF8383052.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            13091..14287
                     /locus_tag="ACGE36_000632"
     CDS             13091..14287
                     /locus_tag="ACGE36_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFF8383053.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14388..15764)
                     /locus_tag="ACGE36_000633"
     CDS             complement(14388..15764)
                     /locus_tag="ACGE36_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8383054.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15801..16667)
                     /locus_tag="ACGE36_000634"
     CDS             complement(15801..16667)
                     /locus_tag="ACGE36_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8383055.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(16756..17781)
                     /locus_tag="ACGE36_000635"
     CDS             complement(16756..17781)
                     /locus_tag="ACGE36_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8383056.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17799..18473)
                     /locus_tag="ACGE36_000636"
     CDS             complement(17799..18473)
                     /locus_tag="ACGE36_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8383057.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18657..19550)
                     /locus_tag="ACGE36_000637"
     CDS             complement(18657..19550)
                     /locus_tag="ACGE36_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8383058.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(19624..21321)
                     /locus_tag="ACGE36_000638"
     CDS             complement(19624..21321)
                     /locus_tag="ACGE36_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8383059.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(21314..22627)
                     /locus_tag="ACGE36_000639"
     CDS             complement(21314..22627)
                     /locus_tag="ACGE36_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8383060.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(22627..26896)
                     /locus_tag="ACGE36_000640"
                     /pseudo
     CDS             complement(22627..26896)
                     /locus_tag="ACGE36_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         7606 a         5920 c         4776 g         8726 t
ORIGIN      
        1 aagacaacta atttgcgtcg tccttacaaa gttagtcatc taacaatagg ccgtttcaaa
       61 actagttgtc tttttatatc cacagatcat tcgacttttt cgaataaccc acggctaatc
      121 atcccatcca tcagaaaata aaatttatct tggctcattt tctgttttgg atctttgaaa
      181 atattgactg ctttcaatcc atttgctgta gtgatcgaca ttttcaatgt ttgaatatct
      241 tttgatacag tgatttttaa tttgaatcct tctttgcttt gcggcgtggc atctaatggt
      301 cggatcaatt gataattgcc tccgtttgtt tctgtaaatc cattatcgcg caatgtatat
      361 ctttttgcat caggatgcaa acgatagcta attggtgcgc ccagtaccgt tgctgttgtt
      421 tcaaatgcca tgattttcac ctcgataaaa ttagtttctc tttattataa aagtttatcc
      481 tgatttttac taggtttttc agttaagttc cctaaaaaat gaaggcgttc tctcagacat
      541 ctgttcatat aagaatgact tcactagtta ttttttgaca gcacgaacta attgatcaag
      601 gaaatactgg atgtcgtctt cggtatttcc caaaccaaag ctgatacgga tggattcttt
      661 tagtatcggt gtatcttctc catacattgc ttctaatacg tgtgaaggct cgatatttcc
      721 tgctgtacaa gctgaaccag tagaaatagc gaccccatta agatcaagct tcatcagtag
      781 caaatcattt gcttcgccaa ttaagcgaag attcaacaca tgagggagtt tattcgttaa
      841 atcaccattt acttgataag ggatttcgtt tgcttctaat gtatcaagaa tcaattgctg
      901 gaatccttta tatttgttgt tacgtgcttc acgttcttca ggtgacagca gttcaactgc
      961 ttttgccata ccccagatcc ctgctaaatt ttctgtacct gcacgccgct tttcttcctg
     1021 ctccccgcct aaaagcaatg cagggatatt tactccgtct cgtttatata agaaaccgac
     1081 gccttttggc ccattgattt tatgagcaga gatacttaat aaatcgattc ctaattcttc
     1141 tggatggatc gtttggtttc cgtacgcttg taccgcatct gtatggaaat aagctggatg
     1201 atccgctaaa agttcaccga tttctttgat tggcatcaaa tttccgattt cattgttccc
     1261 gtacatcaca gagaccaaga tcgtatcttc tcgtaacgct tttttgacct cttcgatcga
     1321 cagattccct ttttcatcta caggcagata tgtaacgtca aacccctttg tctctaaata
     1381 tttcatggta ttaagaacag caggatgttc gatagctgta gtgattagat gtctgccttc
     1441 tttttgacgc gagaacgctg tttctaagat agctgtatta tccccttctg ttcccccact
     1501 gttaaagatg atctcgtgag gttttgcttg cagactgtct gcaatgattt gtcttgctgt
     1561 ttccaatttt tcatgcgcgt ggcgaccaaa tccatgtatg cttgaaggat ttccaaacgt
     1621 gtcttgcatg attgccgtca tcgcttcaat tacttgtgga tgcatggggg tagttgccgc
     1681 atgatccaga taaatagttt tcacttcatt catcccttcc aaattattgc taggcacatt
     1741 ctagcataaa atagcgaaca aaccaaagaa gagctcattt ttgtctagag gacgtttcat
     1801 gggcatagaa aaaaggagct gtgacaaaag tcttgtcgca gctccttgaa gctgctcact
     1861 tctgtcacaa cttctttccc tctttttttt gtttattcga attctttttc ttccagacgg
     1921 aataacggac tcatcggtgt attttcatgg atgcgtttga ccgcttcacc cattaattct
     1981 gaacatgtaa cgattgttaa gttttctgga tgacgatttt cttctgtgaa aacagaatca
     2041 gtgatacaaa tatctttgat tggtgcattg tctaaaatac ttttcgcatg atcggacaat
     2101 aatccatgtg acgcacaggc atatacttct tttgcgccgt tttcttttaa tacatttgct
     2161 gctcgtgcaa gcgttgctcc tgtgttcaag atatcatcta ccatgataca tgtctttcct
     2221 tgaacatcac cgatcacata accaccctca gttgaagcat catctgcatg atctacgatg
     2281 gctaatgtgc tgttcaaata ttctgaaaga ctacgtgcac gctgaacacc gctgtttttt
     2341 ggcgaaacaa ctacgatgtc atctcccgtc agacctaatt gacggtaata atgagcaaac
     2401 aacggcatcg taaataaatt atctacagga atatcaaaga atccttgtac ttgaaccgtg
     2461 tgcagatcta aagtcagtac tcgagttgcc cctgcttctt cgatcaagtt agcgatcaat
     2521 ttcgcagtga tcggttcatg aggtttcgcc gtgcggtctt gcctagcata cccatagtaa
     2581 ggcaatacga cattgatcgt tttcgcactt gctcgcttca ttgcatcgat catgatcagt
     2641 agttccatat agtaatcatt gacaggtgca ttcgttgatt gaacgatata cacatgatcc
     2701 cctcgaatac tttcttcgat attgatggaa atctcgccat cactaaattg cttcacgacg
     2761 cttttaccta attctgttcc gaacactttc gcgatttttt cagctaatgg acggttgcca
     2821 ttcaaactaa aaatcttcaa cgtgtcatcc tgatacttat tactcatgta gtcctcccca
     2881 aaacttctct attcttcgat agtttaacat atttttccaa gattttctgt taatttcagc
     2941 aaattcttaa ggaagtgaaa ctgttccgtt actccaactg gcaggaatga ctttatgata
     3001 atcgccccag ttgtcgtgta ctttgtaata acgttctcct gtttcagttt caaaataagc
     3061 gtacgctgtc acccaatgat tgccatatgt actgccaagt actttcaaaa taccaatcat
     3121 cacaggtttg ccttcacgta tacgttttgt agctcgctgc catgaaccta gaagagttgc
     3181 tcgtgcacgc tcatgattgc cattcttctt taaaaaacta gaaatccctg tactaacctg
     3241 aaaaggaatc gtaggaagtc ctagtggttg gatctggcta cgcaatgcct tcgtcaaaac
     3301 caacgaatca gccgtatttt tcttgcgaat ctcatttgga agcatccacc ccttacgata
     3361 gtcttgataa taggcaagga gaactgctgc cgcgtaagta ccacaaagat agcctgaggg
     3421 ggtagcccat ttcttatacc tcaaaaaacg ttcttctggc aatcccaccc agcaatttgc
     3481 gagttccttt cctgaccttt tttcttcgat caaaacggga atatcttctt ttctgttgat
     3541 ccatctagat aactcgattg gctcttcatt gatcagcttc cattttttac aagaaagacc
     3601 ctcttgataa gtatattttg ctggagaata ttctaccgga tgtcctgatt gatcgataat
     3661 cacataactt tgatcttcaa aaaccagagc taaaaaaagc aatttacttt ttcttgactg
     3721 cagctgctga gtgctggcta attttttaat ttttttatct actttgaaat ataaaggttt
     3781 atccactact tgattcagtt gggatcgcct cctttttttc attataagtg aaggattgaa
     3841 aaaaacaaat cagtttgatt gttcgaattt tcaatttttt atgttctatt tgttaattcc
     3901 ttcattttgt aagataatgg gcacacgaaa caaaaaagga gcgatcgatg aatgaacaaa
     3961 gaacgattga aacaagattt ctatgaagca gtcaatgaag aatggcaaaa aacagcaaaa
     4021 atccctgcag ataaaccagc tacaggcggt ttccaagatt tagttgacgg gatcgaccaa
     4081 cttttgatgg gagaaacaga cgatctgctt gcccatccag aaaaagtgtc ttcagaaaaa
     4141 atgaaacatt tccttgcata ttatcaactg gcaaacgatt atgaccgaag aaatgagttg
     4201 catgctcaac cgcttcaacc tataatcgaa aaagttgaat cattgcagtc tttcgacgaa
     4261 ttgaataagc aatttccaga atggctgttg gatacattac cattgccatt taatctggat
     4321 gtagatgcag atatgaagaa tgcgcaaacg aacgccttct ttgcttatcc gccttcttta
     4381 ttccttccag ataagacgta ttatgcatca gaccatccca acggtgcaca actaataggc
     4441 gttttctttg atatggctgt tcagttatta gagcttgccg gcaaagaaaa aagtcaggca
     4501 gaagcaatca cggaacaagc agtgcaattt gataaattga ttgctcctca tgtaaaaagt
     4561 gcagaagaaa acgcagatta cagcaaaatg tataatccgc aatcttttga tacatttatt
     4621 aaatacacag ataaactgga cttgaaaaaa cttgtcacgg gtttgattgg gacaactcca
     4681 gataaagtga tcgtaactga tccagtgtac ttcgatcatt tgaatgacat cctgacagaa
     4741 gctcattttc cgttattcaa aagctggatg atcattaaaa cagtacgttc tcttagtggt
     4801 tatctatcag aagaattccg ccaagtcagc ggaatctatt ctcgcgtatt gtctggaaca
     4861 gacgaagcaa tgcctcagaa aaaagcagcg tattaccttg cctctggaca attcgatcaa
     4921 gtagttggcg attactacgg aaaaaaatat tttggcgaag ctgctaaaaa agacgtagaa
     4981 caaatggtga aaaaaatgat cgccgtttat aaaaaacgtc tagaagaaaa tgactggctt
     5041 ggggatgcaa caaaagaaaa agccgtcata aaattaaata acttaggtat ccaagtaggc
     5101 taccccgata agatcccagc attattcgac cagttcaaaa cagtacctgc agatcagggt
     5161 ggtactttgc tttcaaatac tcttgttttc agcaaactca cattaaaaga tcgtttcagc
     5221 aaatggaaca aaccagtgga tcgttcagaa tgggaaatga gtgctgacac ggtcaatgct
     5281 tattatcatc catttcggaa tgtgatcgtc tttccggcag cgatccttca agcaccattt
     5341 tacagtctag aacaatcaag ctctgctaat tatggtggaa tcggagctgt tatcgctcat
     5401 gagatttccc atgcatttga taataacggc gctttatttg acgaatatgg caacttgaac
     5461 aactggtgga cagaagaaga tcttacacaa tttcaaaaca aagcacaagc aatgatcgaa
     5521 gaattcgatg gtctgccttt tgctgacgga aaagtaaacg gaaaattaac cgtttcggaa
     5581 aatattgctg atgcaggcgg tctaagctgt gcattggaag cagcaaaaac ggaatcagat
     5641 ctttctatcg aggaattctt tgtcaattgg gcaacgattt ggcgtacaaa agcaaaaaaa
     5701 gaataccaac aacttttact gcaaatcgat gtccatgccc ctgctaaact ccgagcaaac
     5761 gtgcaaccaa aaaaccttga cgaattctat gaaacatttg gtatcacgga acaagacgct
     5821 atgtatctag cgccagaaaa aagagtgcga atctggtaag aggttgtgcc agaagtggtc
     5881 agcttcaaga aataagaagg aatttgggaa aatcgcttct caaatttttg tgacatttcg
     5941 gcttattttt cgaagaagtt acttctggaa taccatgtat tagaggttgt gaaaaagctg
     6001 cttagtttca agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat
     6061 ttgacttact accgagaaac tggcttttga acaccgttta tcgagaggtt gtgaaatcag
     6121 cgtcctgacc tgagtattag agaaaaaaac ggaaaaatag ttcctcatat tttgccgttt
     6181 gttgagctaa taccgagggt cagctgattg aacaccgttt agtggagaga ttgtgaaaaa
     6241 gctgtcctgc atcaagtaat aagaacagcc aatcaaaagc agcaaggaga acctcatcta
     6301 taaaaagata gaaggaagtg agacatctat tggtctcatt tccttctatt tttactttcc
     6361 tcagtcaatt gttttacatc ataaaaggag aaattccact tatttttttc gctgtttcat
     6421 gacataaaca actacaccga ttaacaaaat tcctccagct aatgctagca acgggttatt
     6481 aaattctccc gtttttggga gtccttgagt gccatttttt tcactttctt ttgctgtcct
     6541 atccggatga gaaggttttc catttggttt tccatttggt gattcatttc ctggattttc
     6601 tctttctggt ttgtcatttc ccgggggcac atcggcagtt tctcgcacag tgatcgcttt
     6661 ttgctgggta acagattctg tatgagcaga ccatttttcc ccatcaaata tttccgaacg
     6721 ataagtgact tctagcgtat attctccttt tgaaggtgct gaaaaaatag cggtatgatt
     6781 agaaaattga gcacttgact gtgtttcaac gatttggatc ttttctggga aataacgcat
     6841 atcttcaaag acaggcgttc cttgatttgg tgtgaaagtt gacacaaaaa ctgtaaaagg
     6901 cttcagttca atgatatttc cttctgcatt ctccgccaca ccatttgctt cttgtataat
     6961 aatcggatta ttttgttcat ttggttttgt ctgatttggt tcttgtacga ttgcatactc
     7021 tggatattcc gaagacggtg aggtttctcc gtcaaaaata gcacctacag ataatggaag
     7081 accatccact ctttcctttt ctgtttgacg attccccggt ttaatctgca tcagtccatg
     7141 aacatggata cttccatttt catagcgcgt aacaggctgc tgaccttctt tcgaacatgt
     7201 atttagagac tcgatccaat cttttgatat ggtatgtcca agagtatttt ctgattgttt
     7261 ctctcgttca ttccaatagt agttcaacga gtggtagatt tgatcttcgt tttgattcaa
     7321 tgggcggatc tgctctctca tctcaagatt ctctgttcgg aaagataaga taccagaagc
     7381 atggtagtct gtgtttcctg ctgaagaggt ataaagagcc acattatatg aaccgttatt
     7441 aaaacttgta ctgctattga cgataatatc tgttccgcta ttggaatcga ttcctttcgc
     7501 aagattttcg aaagacaaac tattgatcaa ttcatgaggg cccgttaaag aagaaccgcc
     7561 cattttgaat ccattaccgt taccttctcc ctcaattcct ggtacccagc cgtttcgata
     7621 agcaactgag tttttgatga ttacttttcc gatcgaacct gtttcatttt tagcaaaaag
     7681 gtcccaaccg tcatctgcat tatgatacgc gatacaaccg tcaaatacat ttccttctcc
     7741 tattgtcagt ttggccgcaa atccatcggc atcttcaaat cctggatcgg cattttcaaa
     7801 agatgtacag ttcttgacta agttatgcgc gggccatgct tcgaaaggat catttgagct
     7861 accgctgatt tggattcctg tatttccatt ccgataagca tgaatctctt cgatcaaatt
     7921 atgatttcca gaaatctgta atcctttttg catagctgca ctatttgtaa tgtcaaaccc
     7981 tcgtaaaccc cagtagtgac tgaacagtgt catcccattt ccagttcctt taaaatcaag
     8041 tacaggacgc ttgttttctg gatttttctc tgcaatcaac tgaatcgggt tttctcttgt
     8101 cccgttaacg ccttttggaa tcgtcaatcc tctttcgaag gaataagttc caggcgccat
     8161 gacgattgtc tgcccagcat atgcgtatct caatgcttgt gtcagagata atggattttg
     8221 tctgcttccg tttcctgcat ctgacccttc tggtgttaca taaactgtcg aatatttagg
     8281 tttacggtag tccactgtat gaagaatctc ttttgtttca taactagtca tctctacgta
     8341 ttcttctggc ggatagtttt tatctggcgt aaaactgaaa gtaaattcgt tcgttccttc
     8401 catcagtttt gtctgataat caaattcccg attcgcagtg atagccaagt tttttgcgtt
     8461 cttaagcacc tctcctgttg ctttattttt aattgtcaat aatccgtcag catttgctct
     8521 aaaggacata gtatgatctt ggtttcctga atgattacta gaggtcacag tgtaaatcgg
     8581 atcaatcaag cgagtaggtc tttcttgccc aggaggatct gtcgcaggat ctgagtaagc
     8641 aacttcgatt ccttctacct tgatcgtcat attccttgct gcagcaaatc ctgcataaat
     8701 aaccgattca tctgaagcgt ataatttttc ccagtcatac atgatgtcag aagccatttc
     8761 ttctcctgtt tcaggatcgt aataatatgc ctctaaacca gtattcgttt ttttcatctc
     8821 tacgtccagt ttagtaatcg agttcgtggc cggaaacaga ttcccatgac cgtttccgaa
     8881 tatattataa gaacctgttt ctttttccaa gaaccctgct gaagtctcta aaggtgttgt
     8941 ttccactgag acactcccag gagctggcgc agcttgcgga tcttcagatg atattcctgt
     9001 gattgcccgt gtccccaatc caagtcgcat cgtatatttg ctgccgctcg tgttgaccac
     9061 ttcttgtcta tccgagtccc agacatattc gatccgactg cctagtatgg caaatgaatt
     9121 tgaataaaat gcctgcccaa acaaatgatt ttttggtact gcgtctctta ccattaaagc
     9181 aaatccttct tgggcattcg tataggtcca agattctatt tcgatcgtcg cccgcatcct
     9241 aaagttgatg tctttgggga ttgcttgata ataataggtc attccatctg atccgcttga
     9301 ctggaatttt ccgccgttat tcgtggaaga gagccgcacg ccgtcttcta atggttcaat
     9361 ggcataccta ctagcacttg tgctttctcc gaaaacaacc cctttccact cttcatttgc
     9421 ttcttcagat tgcttttttg tcttttcatt ttctgattct ttcatcatag gatcatttcc
     9481 ttcttccgta tccatatctt cactagttct tggtatttcc tccattgttg acatcccttc
     9541 aatgttcacg tgtgcatttt cttcgttcac agactgttgt tcgttagcag caacttcaac
     9601 gacagaaatt aaaaccgggg tactgagcat aaggagattc atcattagcg cactgtattt
     9661 tatacgtttt tttaacaagt gtattcctcc cgtaacaaat tagaaagcgt ttacttaaag
     9721 tattacctaa tttattttta ctgtcaataa aaaacaaaat tataataagt gtattttata
     9781 aaaagaaaaa atataactta agttgttgat aataaagaga tttattaacg taaaaagaat
     9841 aagttttttt aaaataacgt ataaaaaaga gcataaaaca aagtcgtttg ttttatgctc
     9901 ttcttttatt attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc
     9961 tgcgcctagt gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact
    10021 tgccacggca tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt
    10081 atgcaacaca cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc
    10141 tcgcaaacgg atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca
    10201 ataaacattt tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc
    10261 ccgtccaccc gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga
    10321 atcttttgga tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt
    10381 ctttgtatga gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt
    10441 tccatcctct gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt
    10501 cctccttata cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg
    10561 tcttcgataa attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca
    10621 cgtacgatca aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc
    10681 atttcgcaag ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag
    10741 tcgacaaatg tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt
    10801 gctgctttaa ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct
    10861 ggtaattgtc ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg
    10921 tctgatataa cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta
    10981 ttgatcacca tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag
    11041 acttttccta taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata
    11101 aataaagcgc ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt
    11161 acttcttctt ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat
    11221 ataaatgcga ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc
    11281 atcaaaaaag agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt
    11341 ggtttattct ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga
    11401 taagctgttt ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc
    11461 ttgcgaagtt cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt
    11521 cctttggtca atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac
    11581 tgatctttca tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg
    11641 tcttcatctt gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga
    11701 aggacttcat ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc
    11761 gcacgatagc cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct
    11821 tctcctggat cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga
    11881 aattgtttga caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca
    11941 ggatgaaata ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca
    12001 gcaacatcaa taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat
    12061 tgctcattgt tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca
    12121 tttttcaatt tttctcaaaa actccttatt agtttaccat agataaacct tagattttct
    12181 ctaaatatag aaaatccttc tttagtccga tactctttat agtagtccgt atgctaaaat
    12241 aagtatgtaa tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt
    12301 tattatgata aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt
    12361 ccgaaacaat agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt
    12421 gtggggactc tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac
    12481 tgacgaactt gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt
    12541 tcgacaaaca atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca
    12601 gacagccaaa cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt
    12661 attcaaagaa ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac
    12721 gcatctgccg aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat
    12781 caaaaacaaa cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc
    12841 agctttaatc gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt
    12901 agagatatct gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa
    12961 ggattaactc ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt
    13021 cgagtacttt ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga
    13081 ggaatttttt atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac
    13141 acttgaagat ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca
    13201 acaagcggag attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact
    13261 accggcggaa cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc
    13321 acagtctgtc atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc
    13381 gatgttgaat catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact
    13441 gatgtatcgt ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt
    13501 ccagcgtgtc ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa
    13561 gattggtgca caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct
    13621 aaaagataat ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact
    13681 gaatatttat atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc
    13741 agaagcgatg aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga
    13801 ttacacgcaa ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca
    13861 gatcacgatc cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc
    13921 tgaaaagatc caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat
    13981 cgcattgacc ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac
    14041 aacaaacgac ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc
    14101 gaaagaaaat gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt
    14161 gatcgaaaca atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca
    14221 tgcagaagta gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac
    14281 ccaatagatc acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact
    14341 gttgatgggc gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc
    14401 atcttgctgt gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata
    14461 ccgaacacga tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaagatg
    14521 gagacgattg gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca
    14581 aacatttttc tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg
    14641 atattcgtta gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc
    14701 acaccagcgg cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata
    14761 aacttaggaa gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc
    14821 atcgagatcg ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact
    14881 gttccatgtc ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta
    14941 tacggaacga ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt
    15001 atcgaaccaa tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc
    15061 attgcaacgg catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca
    15121 ccggaaaaac caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct
    15181 acaatgataa acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt
    15241 aatgctttct tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca
    15301 atcaagtcaa tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt
    15361 tcggccccta aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag
    15421 gaaaccgcta gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca
    15481 ccaaatacaa catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat
    15541 tctgcgaaac ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac
    15601 agtgctggac cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg
    15661 atgatcgcac caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca
    15721 ctcgtatgat tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat
    15781 tttaggttgg aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa
    15841 atgactaaaa ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca
    15901 aggatgaaaa tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca
    15961 acagctaaac caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat
    16021 aatccacaga tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag
    16081 aagcttgcgc ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa
    16141 ttcgcccagt tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct
    16201 ctaaaaccct taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct
    16261 tgtcgggcag tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca
    16321 taatctttcg tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt
    16381 aattgaaaac cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg
    16441 aactgtccat tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc
    16501 caaggggtga ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg
    16561 atcgtcattc ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca
    16621 atactacccc atgccaacat agggactaat gctattaaaa tttccataac caatctcctt
    16681 ttatttcaca tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc
    16741 gtttctaacg attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt
    16801 cattgttgtc ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc
    16861 cagatttttt gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt
    16921 tctcccagta aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc
    16981 tgaatcttta actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt
    17041 tccgtgatcc ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat
    17101 ccatttagca tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt
    17161 ttggataaaa taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata
    17221 ttcttggatg aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc
    17281 tacgtattca tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat
    17341 atcgaactcg atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg
    17401 gacccatgag tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac
    17461 ttcttggtgt tcacggatca gctgattgat atattcttct accagttttt cttcttctgt
    17521 cctcccacca ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc
    17581 ccaccatctt ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc
    17641 aatcaaagct aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat
    17701 cacaaagatc gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt
    17761 caaatattta aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt
    17821 cctacttttg tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga
    17881 ttgatgttga ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc
    17941 agcaattttt cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga
    18001 tattcatttt tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca
    18061 ttcaacgtaa tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa
    18121 cggcgaagta atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca
    18181 aaatcatctg cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag
    18241 ataaaaatga tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc
    18301 aaaacgggaa gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag
    18361 aacacttgat tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc
    18421 atgatcaatt gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat
    18481 ccctccatct ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct
    18541 tacctggaaa atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg
    18601 cgtttgtctc ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat
    18661 ctgttatttt tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa
    18721 agcgttagca ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt
    18781 tttgtctgtt ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact
    18841 agtaatcggt gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc
    18901 ttttctctaa ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact
    18961 tcataagcga gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct
    19021 tcaagatccg taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt
    19081 gtactctctc ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac
    19141 aaaagatcat tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg
    19201 gctccgatca tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct
    19261 tgctcaaaag gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc
    19321 tgatttttct tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca
    19381 agctgctttt gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct
    19441 aaaaatggat atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt
    19501 aataaaagac tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc
    19561 ttcttattta aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt
    19621 tgcttatact tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa
    19681 acttattccg attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc
    19741 gcgtttcata ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc
    19801 ttcattatag cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag
    19861 tacataacct tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg
    19921 aacctcaaag tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca
    19981 ctcaattcta tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat
    20041 tgcgtattca gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact
    20101 gcttgatgca tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc
    20161 tactccttct tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt
    20221 atggaaatca tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc
    20281 tcctccacgt gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc
    20341 tgtcaaaatc gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt
    20401 tgttaaaatc gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac
    20461 tgtaggcgta tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga
    20521 atcgatgaaa taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag
    20581 atcaatataa gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag
    20641 gtcgctttcc tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa
    20701 atgcacatct gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat
    20761 atcaggcatc atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc
    20821 tggcgaataa gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt
    20881 cacttggacg gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact
    20941 tgtcagtcga tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc
    21001 ttcggcagaa ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc
    21061 gtaatatact tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc
    21121 aaaatccgta gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt
    21181 tttgtgtaat gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag
    21241 ttttccgttc ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt
    21301 gacaatcatt tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac
    21361 ctaacaataa aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga
    21421 atacggacca ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt
    21481 ccgttgtttc ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga
    21541 ctggaacttt cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg
    21601 gttggccttt tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt
    21661 catccatctc gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca
    21721 agaaacgttg tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt
    21781 tgtcattcaa aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag
    21841 gagccatatc taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac
    21901 cttctaatgg ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg
    21961 gatctcttag ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg
    22021 attctgtcgg gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat
    22081 caacatccaa tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc
    22141 gatcttggtt tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg
    22201 tatctttccc agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct
    22261 gacggatatt gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt
    22321 tttcagcagc ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc
    22381 catttagtgg caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag
    22441 ccaagccatc attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc
    22501 gtttatgcaa cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg
    22561 gaagaaggac tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct
    22621 ttttcattac cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc
    22681 tgcaagcgcc atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt
    22741 agcaggcaac ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt
    22801 ctccaaaagt tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg
    22861 ataatacgaa atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt
    22921 ttctatgtca ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct
    22981 atagctgtca ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc
    23041 attgaatagg gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc
    23101 tcctgcacta ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat
    23161 actgtcaatt ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg
    23221 tagtacacca tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac
    23281 cgtatcttca atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga
    23341 tagccgatac ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg
    23401 cagaggttgg tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa
    23461 gtatccgatc ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc
    23521 tcgatacagc gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa
    23581 tgtatggcct tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta
    23641 agcagtgaat gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca
    23701 attttcttca cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa
    23761 gatccatgtg tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat
    23821 tttgtattct gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc
    23881 tccgattgga tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac
    23941 tgaatagaac gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt
    24001 cgctactgga tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc
    24061 gccattgatt ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt
    24121 gaattcattt cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt
    24181 aggctcttgt acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt
    24241 accttccggt ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc
    24301 taacatctca tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac
    24361 taactgataa cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc
    24421 agtttcatct cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt
    24481 tttacttgtt tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa
    24541 ttcgactcca gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc
    24601 tttatgttcg taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt
    24661 ctcataatcc tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc
    24721 ttgagctccc gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa
    24781 agaaatcgga gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc
    24841 cactacttct ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta
    24901 cttcatgccg ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc
    24961 tccaaatggt gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct
    25021 cttgaaggta gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt
    25081 gtaatatcgc ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg
    25141 tccaatagca tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga
    25201 gaagatactt ggcgggttct cttgatactt gtttttcagc caatgatgat aagcaactgt
    25261 gttgatcgca gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga
    25321 accatcacca acccagtgag tcgctttatt atctccttct tttacctctc cataacgata
    25381 gtgtacattt acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt
    25441 aaaccctgca ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt
    25501 atcttcagca atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt
    25561 acgataactt gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat
    25621 taggattttt tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc
    25681 atttttaagc cctagtgtaa gcggagttcc tccgattggt gtacgattta atgctgtatt
    25741 ctcacttccc gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg
    25801 ctcatggtta ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc
    25861 cagactcggg tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc
    25921 atttttagtt tgactattgg tcccagcggt taattcactc atactcgctg acttatctag
    25981 aacaaaaacg atatcgattg gactgatttc ctttttctcg cccccgatga catctagata
    26041 gacattgtaa agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag
    26101 aacaggttct acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa
    26161 attccgttca tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc
    26221 tgcagttgat ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt
    26281 ataatcacta tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata
    26341 gcgttcattg ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt
    26401 catctattct ttcatcaacc accacttgaa gaggtagtta cccttcgttc acatcgggga
    26461 tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga
    26521 cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg
    26581 aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga
    26641 ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt
    26701 caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga
    26761 atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat
    26821 agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca
    26881 attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa
    26941 tcaacaaaac aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga
    27001 tatccttttt agttaaaaaa caccaatt
//
LOCUS       DAXWLH010000021        26907 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXWLH010000021 DAXWLH010000000
VERSION     DAXWLH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.021"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..584
                     /locus_tag="ACGE36_000641"
     CDS             <1..584
                     /locus_tag="ACGE36_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383061.1"
                     /translation="ATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDITEAEENPFFL
                     ILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFNHNTLFFFI
                     MNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNPSTI
                     QVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(718..843)
                     /locus_tag="ACGE36_000642"
     CDS             complement(718..843)
                     /locus_tag="ACGE36_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFF8383062.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(977..1870)
                     /locus_tag="ACGE36_000643"
     CDS             complement(977..1870)
                     /locus_tag="ACGE36_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFF8383063.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2052..2978)
                     /locus_tag="ACGE36_000644"
     CDS             complement(2052..2978)
                     /locus_tag="ACGE36_000644"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFF8383064.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3063..3824)
                     /gene="pflA"
                     /locus_tag="ACGE36_000645"
     CDS             complement(3063..3824)
                     /gene="pflA"
                     /locus_tag="ACGE36_000645"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFF8383065.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4068..6299)
                     /gene="pflB"
                     /locus_tag="ACGE36_000646"
     CDS             complement(4068..6299)
                     /gene="pflB"
                     /locus_tag="ACGE36_000646"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFF8383066.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6576..9026)
                     /gene="parC"
                     /locus_tag="ACGE36_000647"
     CDS             complement(6576..9026)
                     /gene="parC"
                     /locus_tag="ACGE36_000647"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFF8383067.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9038..11083)
                     /gene="parE"
                     /locus_tag="ACGE36_000648"
     CDS             complement(9038..11083)
                     /gene="parE"
                     /locus_tag="ACGE36_000648"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFF8383068.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11249..11683)
                     /locus_tag="ACGE36_000649"
     CDS             complement(11249..11683)
                     /locus_tag="ACGE36_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFF8383069.1"
                     /translation="MSVKNPTQETIFNYLEETKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11810..12448
                     /gene="plsY"
                     /locus_tag="ACGE36_000650"
     CDS             11810..12448
                     /gene="plsY"
                     /locus_tag="ACGE36_000650"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFF8383070.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12580..13455)
                     /locus_tag="ACGE36_000651"
     CDS             complement(12580..13455)
                     /locus_tag="ACGE36_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8383071.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13537..14328)
                     /gene="codY"
                     /locus_tag="ACGE36_000652"
     CDS             complement(13537..14328)
                     /gene="codY"
                     /locus_tag="ACGE36_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFF8383072.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14346..15746)
                     /gene="hslU"
                     /locus_tag="ACGE36_000653"
     CDS             complement(14346..15746)
                     /gene="hslU"
                     /locus_tag="ACGE36_000653"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFF8383073.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15760..16308)
                     /gene="hslV"
                     /locus_tag="ACGE36_000654"
     CDS             complement(15760..16308)
                     /gene="hslV"
                     /locus_tag="ACGE36_000654"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFF8383074.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16345..17250)
                     /gene="xerC"
                     /locus_tag="ACGE36_000655"
     CDS             complement(16345..17250)
                     /gene="xerC"
                     /locus_tag="ACGE36_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFF8383075.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVIYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17448..18824)
                     /gene="trmFO"
                     /locus_tag="ACGE36_000656"
     CDS             complement(17448..18824)
                     /gene="trmFO"
                     /locus_tag="ACGE36_000656"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFF8383076.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18793..20871)
                     /gene="topA"
                     /locus_tag="ACGE36_000657"
     CDS             complement(18793..20871)
                     /gene="topA"
                     /locus_tag="ACGE36_000657"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFF8383077.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20993..21850)
                     /gene="dprA"
                     /locus_tag="ACGE36_000658"
     CDS             complement(20993..21850)
                     /gene="dprA"
                     /locus_tag="ACGE36_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFF8383078.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21906..22673)
                     /locus_tag="ACGE36_000659"
     CDS             complement(21906..22673)
                     /locus_tag="ACGE36_000659"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFF8383079.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22666..23526)
                     /gene="ylqF"
                     /locus_tag="ACGE36_000660"
     CDS             complement(22666..23526)
                     /gene="ylqF"
                     /locus_tag="ACGE36_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFF8383080.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23786..24184
                     /locus_tag="ACGE36_000661"
     CDS             23786..24184
                     /locus_tag="ACGE36_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8383081.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24421..24963
                     /locus_tag="ACGE36_000662"
     CDS             24421..24963
                     /locus_tag="ACGE36_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8383082.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25046..25597)
                     /gene="lepB"
                     /locus_tag="ACGE36_000663"
     CDS             complement(25046..25597)
                     /gene="lepB"
                     /locus_tag="ACGE36_000663"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8383083.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25749..25976)
                     /locus_tag="ACGE36_000664"
     CDS             complement(25749..25976)
                     /locus_tag="ACGE36_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFF8383084.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25973..26491)
                     /gene="msrA"
                     /locus_tag="ACGE36_000665"
     CDS             complement(25973..26491)
                     /gene="msrA"
                     /locus_tag="ACGE36_000665"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8383085.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26511..>26907)
                     /locus_tag="ACGE36_000666"
     CDS             complement(26511..>26907)
                     /locus_tag="ACGE36_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFF8383086.1"
                     /translation="VLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNM
                     IKRNYKLPDWIEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTS
                     DASNNSNTNKTSTSSTESKTKENKKSSDE"
BASE COUNT         7110 a         5689 c         4633 g         9475 t
ORIGIN      
        1 ttgcaacttt gacgatctta ttttttctat gttattcata ccttaagacc tcacatgcgc
       61 cttctagtgc attaggctgg ttgcttttgg atcattctga tatcacagaa gccgaagaaa
      121 atccgttttt tttgattttg tcttctcttt gcggcttact gttttctgtt tatttcggtg
      181 cctatttttt tcatcgacat cctggtacgt taaacgaagc ttcaagcgta ttgattgcat
      241 ttaaaacgtt ttctttttta gcaattcttt tttattcgtt taatcacaat acgcttttct
      301 tctttatcat gaatggttta gtcgtgattt tatccatttt tacttatttt attttttcac
      361 tcatttttag ccaattgaaa tgtccgctct tctttcgatt agttcctgtc acctgtttta
      421 tttacagctt gttttccttt tttgtccttg ctttaatgcc actgaccaac ccttcgacga
      481 tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt cagtctaggt gtatttatgt
      541 ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc ctgactatta cgacttcata
      601 aatgcttttt tagttttaaa cacttcaatc taaaagaata acccaagatt ggataaatta
      661 ttaatgactc gccattcgtt tcttagtgat tctggattat caaatggact tcctaatcta
      721 atcagctagt tcttcccata aaatgccata cttgactgaa tcataatatt gattatttaa
      781 aaatctaact tttcttatca ctacttcctt tgtcattcca catttctcac catttttttg
      841 cattcctttg tttccagacc atgtagtgaa tctaataggt ggtagatagg caaaatactc
      901 aaataattct tttaaccata aaggaagaga aaggctcctc ctgcaagagt cagtataatt
      961 tttgcttact gctctcttag gttttactca ctaaaactgt tccatattct ttgatataag
     1021 aaaatgcatg cggtataaat ttcttgctca gctcagaaaa gatttgtcgt ttaagaaagg
     1081 ccatttcctc cattgtcaat gaaggatcca atgttggtaa ttcaatctca aaattgtttt
     1141 tttcgacaat aggtaattct aatgtgaatg attcttcttc ctttatagta ttcgtcacaa
     1201 gtagagactg atggaaaata ctaggccggc gatttttata tttattctta cgtatcctgt
     1261 caacgataac ttctatttga tcaaaaaagt acgattcatt tacgtctcca ataagttcgg
     1321 ctaattcttt gaatgccttc gttacaggaa gctgattctt ctgtacaaaa aagtaattag
     1381 ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat aaaacggtac ttctcatttc
     1441 ctgcagttgt cctcagcaat tctgtctctc ggctgtttct tactccataa aaatacgctt
     1501 ctttccgttc atcttcgaaa caatacgccc aaataacttc tcccatcgct aatttttgtt
     1561 catcaacaga gagtctcttc aattgttcag ctatggtttc agcagccgaa gtggcttgtt
     1621 gcaagtaatc tttaggatca aatattggaa agttcaaatg atatcttcgt tcttccctct
     1681 tgattaatcc tgcatcgatc aataaatcaa gaaaatggga aaatttattt tgcgaaaatt
     1741 cttttttcaa ttctcttaga atcgtttctt gttctcgatg ttcatataga taatcggtca
     1801 atgaagaaaa aagcggatca tcggtcagtt tttgataaaa gttttcctta ttttttgaat
     1861 aatagttcat atatgttctc ttttctatcc gttttatttt acttaaaagg cttttacgta
     1921 aaaaggagga agaacaagag tcataattga ctgttattct cctccatttc ttcggccttt
     1981 agtatgttct atgtttcttt ttatatcaat cgcttatcca ttcattttga atgcttcata
     2041 ctaccatttg attagctaaa tacagccgtc aattgaggta cgacttgttt tttacgtgaa
     2101 acgacacctt taagcaaagc acggttgttg gtcaaagttg tttggaaagc ttcttctact
     2161 ttttcttttc ctttaccagc aacaagtaat tctgaatcac tgtctaagat gtttgtgaca
     2221 actaatacga acagatcgta gccatttgct ttcatttcat cttccattgc tgcttctagc
     2281 tctgcttgtc gatccatcac ttcattaagg tccactgtat tgacttgagc gatacgaacg
     2341 ttggaactgc ccattgggaa actttttgca tccaaatcaa gaagaaccgc agctgattta
     2401 tcacttagat ttgttcctgc tttcaacatt tcaagtccat aagtgtttag atcgacttca
     2461 gcgattgctg caagttcttt tgcagctgct tcatcttcag gcgtacatgt tggtgatttg
     2521 aacaacaatg tatctgaaat gatagctgag agcatgattc ctgcgatttc tttaggaatt
     2581 gttacttggt tttctttata tagttttaaa ataatcgtac ttgtacatcc aactggttct
     2641 gcacgatagt acaatggatt agctgtttca aagtttgcaa tacgatggtg gtctactaca
     2701 gctaaaatat ttacttcgct gatatctgaa atactttgtt ggaattcatt atgatccaca
     2761 agcatgacat ctgcattttc tgctttagct gactctacta ctcgtggtgc agatagctga
     2821 aagtggtcta atgcgtattg tgtttcttca ttaggtgtac ctagagcaac tgcttctgtt
     2881 tctttaccta attcattttg caagtgtgaa aacgcaatgg ctgccccgat tgcatctgta
     2941 tctggatttt ggtgaccaaa aactaaaatt tttgacatgt cattcgctcc tttgtgattt
     3001 ctcctattta ataatagcaa aaaaacggaa agaagcaata cttatcttct ttccgatgcg
     3061 acctatcgcg ttaaatattt tgtatactct tctgtatgaa gcaatcgttt agcattttcg
     3121 acacgttctt ttgtaggtgg ttcgatccct tctaatggat atttcaaacc aagtgtttcc
     3181 cacttgtatt ttcccatcgt atgataagga agaatctcta ctctgtctac atttgacaag
     3241 gtttgaataa agttgtctag tcggatcaaa tattcatcat aatcacttcg ctcaggaact
     3301 agtacatggc gaatccaaac cggtttgttg atttttgaca aatattgagc catttctaaa
     3361 atattttcat tgtcccaatg cgttagtttt ttatgctctt cattatcgat gtgtttgatg
     3421 tcaaataaca gtaaatctgt atacttcatc aattcttgaa aacgtgaaaa gaaaggttct
     3481 tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat gcatcttccg ctcttttgct
     3541 tttttaaata gttcgatcaa aaaatcgatc tgcaatagag gttctccacc actaacagta
     3601 ataccacctt tatctcccca gtaaggtctg aagcgttctg ctttatctaa aagttcatct
     3661 gctgtgtatt ctttccctcc gccaatattc caagtatctg gattgtgaca aaattggcaa
     3721 cgcatgcggc atccttgcat aaacacgaca aagcgcagtc caggtccgtc tactgaaccg
     3781 aaagtttcga tagaatgaac atagccgatt gttttttctt ccatttaagt tcacatcttt
     3841 tctttgattt cattttaagt acaagttagc tgtcgaagcg aagctttctg aacaaaattt
     3901 ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga cggaacatac aagtcctgcc
     3961 cctgtaaatc gtttctttca aataagcata ctgattattc gattttgatg cgatggctat
     4021 tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac gaacttttta cattctgtcg
     4081 tgtgaagttc tcatcaagac atctaattgt tgttctcttg tcaagtcacg gaatttaact
     4141 gcataaccag atacccggat cgtcaagttc ggatatttca ttggattttc cattgcatca
     4201 cgcaataaat cattactgaa tacattgaca ttcaaatgat aagctccttt tgagaagtat
     4261 ccatctaaga cattacgcaa gttttcgatt cttgtatctt gttcttttcc aagacctgaa
     4321 ggattgattg tttgggtatt tgaaattcca tccaatgcac agccgtaatt cattttagct
     4381 gttgagttca ggctagctaa taacccactt tgttccgcat tgtaacttgg atttgctcct
     4441 ggcgcaagtg gttttccagc ttcacggcca tcaggagtat ttcctgtatt tttaccgtaa
     4501 acaacatttg aagtaatcgt caataatgaa gttgttggtt ttgagttgcg gtaagtttta
     4561 tgacgtttga tttgtcccat gaagtattcc aagatccatg aaactaattc atctgcacgg
     4621 tcatcatcat ttccgtattt agggaagtcg ccatccactt caaaatcaac tgctattcct
     4681 ttttcattac ggattgcttt tacttttgca tatttgatcg ctgacaagct gtctgccgca
     4741 tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca agtcagaatc catcaaagcc
     4801 agctgagctg cttcataaga atatttatca tgcatataat gaatgatgtt caacgtattg
     4861 acatatagtt ctgctaacca atccatcata ttcatgaagt tatgtttcac ttcttcaaag
     4921 tctagtattt ctgatgtgat tggacgatat tctggtccaa cttgggcgcc agtcacttca
     4981 tcgactccgc cattgatggc atataatagt gttttagcca agttagcacg agcgccaaag
     5041 aattgcatgt cttttccgac aactgttcct gatacacagc aagcaatcgc acaatcatca
     5101 gaaccccatt gttcacgaag ctgattatca ttttcgaatt gaatcgaaga actttcgata
     5161 gcgattctgc tagcatatgt tctgaatcct tctggaaggt gttcagaata caaaacagtc
     5221 aagtttggtt ctggtgaagg tcccatattt gttaatgtat gaagcagacg gaaatcattc
     5281 tttgtaacta atgaacgacc atctaatccc atacctgcaa tggataaagt agcccagata
     5341 gggaatcctg agaataattg gttatattca ggtgtacgcg cgaatttgac catacgtaat
     5401 ttcattacta agtggtcgat caattcttgt gcttcttttt ctgtgattac tccattatcc
     5461 aaatcacgtt ggatatagat atcaaggaat gctgagatac gtccgattga catcgcagca
     5521 ccattttgtg atttgattgc tgctaaatat ccgaagtata gccattggac agcttctttt
     5581 gcatttgcag ctggtttaga aatatcatag ccatatttcg cagccatttc tttcatttgc
     5641 gctaatgcac gatattgttc agtgatttct tcacgtaaac gaatcacttc ttcactcatt
     5701 gtatgataac cagtcatatc atgatctttt ttcttttgtt ccatcaaata gtcgattcca
     5761 tacaatgcca cacgacgata gtcaccgatg atacgtccgc gaccgtaagc gtctggaagc
     5821 cctgtaatga ttttgttttt acgagcagca cgcatttcag cagtataagc gtcaaataca
     5881 ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg tcaattcatc gtttggtgta
     5941 tagccattgc tttttagcgc attgttagcc atattgattc cgccgaacgg catgaatgct
     6001 tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt ccaaatcttt atccaaatag
     6061 cctgcatcgt gtgaagtaat agttgatacg atttctgtgt ccatatcata gacaccgtta
     6121 cgctcgaatt ggatatcatt taacttttgc aattcatccc ataatttttc tgttgcttct
     6181 gttgggcctt ccaaaaagct gtcatcaccg cgatattcag tgtagttatt ttgaataaag
     6241 tctctagtgt ctacgccttc tttccatttg gtacctttaa atccattcca ttgctccatt
     6301 gtatggacct cctaaatttc tgttttatgt aacagttttt acgtgattag tataacacag
     6361 caccctatct ttgcaagggt tttcttgttt tttatttaac aatttttatg ttttatttac
     6421 atatatttaa atcaacgttg acataccaac atttaagcat tgtgaacact ttcatctttt
     6481 tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa gagacgccac ctgtattaat
     6541 acagatggcg tctcttcatt aaaatactac aacagttacg actcgatgaa aatctcagga
     6601 gcatcgataa cttcgatgac ttctccacct tttttctcat ccatcgcaaa tgatccattc
     6661 gatgttcgat cacctatagg gaaatcagtt gggtcaatcg ttaactgctg ccctttttga
     6721 ttcatcaagg ttatttcttt tgatcttcct tccgacatat agataatacg gtgcggattt
     6781 tttttcagct cacgcatgac cgtcactccc cgcttcgcac gtgaagtttg gctgatttct
     6841 tgagcaagca tgcgtttaac agctcctcgc tgagtaacga ttacgatcgg cgtatcacct
     6901 tctgctaaca caagcagccc gttgataatc ctatctccat ctttcaaatt catagacttc
     6961 actccagctg cctttgctcc aacaacggga acatctgtta aaggatagcg caaacccatt
     7021 ccttgatgcg ttactaagaa aacatctaag cctggctgat cttctttcag atacacagca
     7081 atcagttcat cttcttccgc ctttagcttc atacagctga gcggacggct cttataggtg
     7141 cgccatggtt cgaactctgt cattcgtgtt tgcttgatca atccattctt gctgatgaat
     7201 acaaatgttt tctctgcttg aattttttga tatgggaaaa cagctaaaat cgattcgtct
     7261 acagacagat tggagattgt ttgagaaatg tgctcaccag catctttcca tttcaaatcg
     7321 ggtagttcat ggacagggcg gtagatcaca tttgctttat ttgtgatcag aagaacatgg
     7381 tccaatgtac tcaattcacc tgagtatagc aagtagtcgc cttctcgcat cccgatttct
     7441 tctggtttgg aagcactata agaacgaagg cttgttcgct tcacatagcc ttctcgcgtc
     7501 acactgacaa ttacgtcttc ttgtgccact aagacttgcg tatcaatttt gatttcttcg
     7561 atttcgtctt cgattgtcgt tagacgagcg gaagaataat tttttttcac ttctcgcaat
     7621 tcttttttca taacagagaa aagctctttg tcatttgaaa ggattttatt caactctgtg
     7681 atttgcgcaa tcaagctttc ggattccttt tgaagctctg tgatatccgt gttcgtcaaa
     7741 cggtataatt gcaatgtgac aattgcttct gcttgttctt ctgtaaattg aaagacgtcg
     7801 actaaattct ttttggcgtc ttttttgtct ttactttcac gaatcgttgc gattacttca
     7861 tccagaatcg ataaagcttt catcaatcct tctacgatat gctgtctttt ttgagctttg
     7921 gctaaatcga attgactacg ttttgttatg acttgctttc gatgctcgat ataacttctt
     7981 aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt cgatagcgac catattgaaa
     8041 ttgtaattga tctgtagctc agtgtttttg aacaaataat tcaaaattcc ttgagcattg
     8101 gcgtcttttt tcaattcgac gacaatttgc agacctgttc tatcactttc atctcgtact
     8161 tcagctattc catcgatttt cttattcaaa cggatctcat ccattttttt caccaaggtc
     8221 gctttattta cttcatatgg gatttctgtg atcacgattt gctgtttatt acccttcatt
     8281 gcctctatct ttgttttcga acgaagaata acttttcctc gtcctgtttc ataagctttt
     8341 ttgatttcat cttttccttg caagatccct cctgttggaa agtcaggtcc aggaatatat
     8401 tccatcaatt tttcaaggct cgcttgtgga tgatcgatca tatagattgt gccatcgatc
     8461 acttcagcta agttatgagt cgggatttct gttgcatatc cagcagatat acctgtcgat
     8521 ccattgacga gaaggtttgg gtatcgagct ggaaggactg ttggttcttt ttctgtatca
     8581 tcaaaattcc acacgagatc gactgtttct ttctcgatat ctgctagcat ctctccactt
     8641 aacttggata atctggcttc agtataacgc atcgcagcag gtggatcacc gtccatactc
     8701 ccgttattcc cgtgcatttc gatcagtact tcccgcaact tccagtcttg gctcatgcgg
     8761 accatcgctt cataaatact gatgtcacca tgaggatgat aattccccat gatatttccg
     8821 acagattttg ctgatttacg gaatcctttg tcatacgtat tgccatcttt gttcatggca
     8881 aataatatcc ggcgttgcac tggcttcaat ccatcacgga tatcaggcag tgcacgttct
     8941 tggataatat atttggaata acgtccgaag cggtcaccca tcacttcttc taaagttaat
     9001 tcttgtactt cttgtcgatt ttccatcagg tgacctccta ttctacttca aataactgat
     9061 tgttttcatt tttatctgct cgttcttcat ctaataaatc attagaaact gaaggagaga
     9121 tttcagtatc ctctttttta tctaagatac tgccatcttc ttcgagcgta aattgcacgt
     9181 gattttcgat ccattttctt cgaggttcta ctttgtcacc catcagtgtg gttactcgac
     9241 gttcagcttg cgcagcatca tcgatacgga cgcggatcaa cgtacgggaa gtcggatcca
     9301 tcgttgtttc ccacaattgt tccgcattca tctctccaag tcctttatat cgctgcagca
     9361 tatatccttt gccgactttt ttaatcattg ctgccaattc gtcatctgtc cacgcatatt
     9421 caatgacttg cttctttcct tgtcctttgg atactttata taaaggcggt aacgcgatat
     9481 atactttccc agcttcaatc agcggtttca tataacgata aaagaaagta agcagtaata
     9541 cttgaatatg ggcgccgtct gtatccgcat cggtcatgat gattactttg tcgtaattac
     9601 agtcttcgat agaaaactca ggaccgactc ctgcaccaat cgtatagatc atcgtattga
     9661 tttcttcatt tttcaagata tcttgcattt tagctttttc ggtattgatg acttttcctc
     9721 gtaaaggtag aattgcttgg aactttctgt ctcttccttg tttagcagat ccgccagcag
     9781 aatccccttc gactaaatac aattcatttt tcttagggtt tcgggattgt gcgggtgtca
     9841 attttcctga aagaagagat tcgccttttt tacgcttctt cccatttcgg ctttcttctc
     9901 tggctttacg tgctgcttct ctcgcttctc tggctttgat tgctttacgg accaacatct
     9961 ggctcatttc gctattttcc tgcaaataaa agcccatttg ttcactgatg acgttatcta
    10021 ctacggtacg agcaactggc gtaccaagtt tttcttttgt ttgcccttca aattgcagaa
    10081 gattttcagg tacacggata gaaagtacag cagctaaccc ttctctgaaa tcactacctt
    10141 ccagattctt gtcccgttct ttcagcaaac caacttttct cgcatattca ttataagatt
    10201 tcgtcatcgc cgctttcata ccagcttcat gggttccccc atcttttgtc cgaacgttat
    10261 tgacaaaaga taaaacattt tctgaataac cgtcattgta ttgataagcg acctctactt
    10321 cgataccttc tttttctcct gaaaaataaa caactggtgt caatgtatct ttttcttcat
    10381 tcagataatc aacaaattct ttgattcctt cttcgtaatg aaaaatttcc ttgactggtt
    10441 cttctccacg aagatcagaa agagagattt taacgccttt tagaagaaag gcagattctc
    10501 ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt actgaatatt gtatcatctg
    10561 gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt tcctatcttt ttcaaagtac
    10621 cgtcaggttt accgccgttt ttaaatttct gctgatactc tacgccatct ctaacgatcg
    10681 ttacaattag ccatttcgat aaggcattta ccacactggc tcctacaccg tgcagtccac
    10741 cagaagtttt gtaccctcct tgaccaaact ttccgccagc atgcagcaca gtaaaaatca
    10801 cttcaactgt tggtattccg gaagcatgca ttcctacagg catcccacgt ccgctatctg
    10861 caacagttat actattgtct tcatggatgg tcacatcaat ctcactgcca taacctgata
    10921 atgcttcgtc aactgcatta tcgacgattt catagactaa atgatgcaag cctcggctgt
    10981 cagtggatcc aatgtacatc cctggtcttt ttctaactgc ctctaatcct tccaatactt
    11041 ggatggacga gtcattgtat tcgttgttta ctttttttgc caaggcaaac gctccttcaa
    11101 taatactttg cggcaaaacc gcaccacgat tatcatacta gaaaaggtcg tgaaaaaaaa
    11161 gagggtagac gagaaatcaa ttttaattat ctcctaaccg ctttttcgac attcccctct
    11221 atcgatcatc tcttctttgt ggtcattttt atttttggtt ttctttcaac ttaccaagtt
    11281 caatcttaat acaacgatcc attacgatat ctgtacgtcc tgcttttctt agcatctcag
    11341 ctgcttcttt gttttcaatc cctaattgtg cccagaacac tttagcatca gttttcaaaa
    11401 aatcacctgc tatttcaggt aaaaattcac tccgtctaaa tacatctacg atatcgattg
    11461 gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc tagaatttct tcaccagcca
    11521 ataatggatt tactggtatg attttatatc cattgtcttg caatacctgt gcaaccatgt
    11581 aacttgttcg ttctggttta ttgctcaagc ctacgacggc aatatttttt gtttcttcca
    11641 aatagttgaa aatcgtttct tgtgttggat tttttacaga catttctgcc accccttgct
    11701 tttcttttta ttccatctat tatacttgta tctttcattt tttcaattgt tcctgttacc
    11761 tattcatgct aaaataaagg atagttttca acttgaaaga gggtaaaaaa tgaaattgat
    11821 cttattactt attttagcat atcttttagg ttctattcct tccggtgtct ggattggcaa
    11881 attgtttttc aaaaaagata tccgacaaca cgggagcggt aatactggaa cgaccaatac
    11941 atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg cttatggata ttttaaaagg
    12001 tacattggct acttgtttgc cgatgatctt ccacctttct gggattaacc ctctttggtt
    12061 tggggtatgc gccattcttg gccatacatt tccgattttt gctgggttca aaggaggaaa
    12121 agctgttgcg acaagtgcag gaatgctttt aggatttaat cctgtatttt ttgtttattc
    12181 ttgtatcatc tttatcgtta gtttattttg tacaagtatg gtgagtttga gcagtatgat
    12241 cagtgcagta ttgatcactt tatctacgat cgtccttcct tatgtagctc ctgtgatttt
    12301 ggctaagccc aattggttac tgacgatcat tgccttttta gtttcttcct ttatctttta
    12361 tcggcataaa gacaatatca agcgtatcag aaaccatact gaaagtcgaa tcccgtttgg
    12421 tttgaatagc agcaagaaaa aatcctgata ctttttcatt ccaaagagat aaaacgggag
    12481 tgtgacaaat cgatgtcaca ctcccttatt acttacagga tagctttttt acacaccttg
    12541 gtaaacggtg ttccctgcta acttctcggt cacaacctct tacttcactg tgatggaata
    12601 ttttgtcttg aagatttcat gggcacctag ttgattgatc cctacttttt ccatcaagtt
    12661 tccactactg ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa cgaaaggcgc
    12721 ttcttttgga taaggagacc agatccctac ataaggcata tctttatagc ttaaagttac
    12781 gctatgggaa gatttttcac ttcgaatggc gtacgcattt agaccccttg tttcaaaaac
    12841 caaggcatct tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg tctgacctaa
    12901 cgtcttttga tccatatcga tgaatgggcc tttcaatggt aggcgtaatc ttgatttcaa
    12961 tggagaaaat gccagataga aatcttcaaa tgacaatcct tcttcaagag gtacattgaa
    13021 agcaggatgt ccaccgatag aaaagaacat ttctttttca ccggtatttg ccacttgata
    13081 atgaaccgtg atgccgtctc ctcctaattc atagctgatc actaattcaa aatcaaatgg
    13141 atacttttct aatgtctccg gcgtactcct taagatgaaa ctagccattt cttgtccatg
    13201 ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt tgccccattt gatatgtttt
    13261 cccttgataa acatactgat catcttttaa acgtccaaca aaagggaata aaaccggtgc
    13321 atgtctgccc caatacgctg gatcaccttg ccagatatac tctaaaccat gttcttttga
    13381 tttcagactg atcaattccg caccgtgttc agcgatttcg gcgatcagct ggtcattttg
    13441 aataataaca gtcattgcca ttcctccaac ttcgacttac tttttaaaat gggaaggctg
    13501 gaacaaacat ttctatctgc cccagtcccc ttacttctaa taactgtgtt ttgctaactc
    13561 gtctttaaaa cgggaattca atacttttaa ataagttccc ttcatgccta acgaacgaga
    13621 ttcaatgatt ccagctgatt ccaattttcg taaagcattg acgatcacag aacgagtgat
    13681 gccgatacta tcggcaatat tagaagcagt cagacgacct tcatctccat ccaatgcttc
    13741 aaagatagct tgtacagcct tcaattcgct ataagataat gtgttgattg ccatttggac
    13801 agccgttgca ctccgtacat ctgcttcgat gcttcgagac ttctgataaa ggatctgcat
    13861 accgacaacc gtagctccat attctgctaa gactagatcg tcatctgcaa acgattgttc
    13921 catacgagat aagataatcg tacccaaacg ttcgccagct ccaaagatcg gaacgacagt
    13981 cgtcagacca aatggatatt tctctcttga ctctaccgga aatgcagtaa tatcactagt
    14041 aattgggata ttagcttcag ttttcatcaa ctgtgccact gtatcagtat aagattcagg
    14101 aaattgtttc tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt gacgttcatt
    14161 ataacctagt aatactccgt cacttcctat gatataggtg ttgctgtcaa gaataccacc
    14221 tagtgtgaca gccattttat cgtaaggcaa atctgcttgc atgtcaaaag tattcttttg
    14281 ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag tctgtcattg tcgtcctccg
    14341 attttttata aaatatagcg acttaaatct tcatttgccg caattgagcc aagtttgtca
    14401 tccacatatg cttctgtgat cgttatttct cccatttgca tatctggtgc ttcaaataat
    14461 agatcttcta gtagtttttc caaaatggta tgcagtctgc gtgcgccgat attatctgtg
    14521 tcacggttga catcgaaagc gatttgagcg atccgttcaa ttgcttcttt tgtaaacgta
    14581 actgacacat tttctgtacc gatcaacgcg acgtattgtt ttactaaagc attatttggt
    14641 tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat ctaattctac ccgtatcggg
    14701 aagcgtcctt gaagttctgg aattagatca cttggtttag acaaatggaa agctcctgaa
    14761 gcaatgaata ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac ttgtgaacct
    14821 tcgacaattg gcaatatgtc tctttggacg ccttctctgg atacttctcc agattgctgt
    14881 gatttagaag tgatcttatc gatttcatcg ataaaaataa ttccactttc ttccgctaat
    14941 ttgattgcct cactatggat atcagcatcc ttgactaatt ttgctgattc ttccttgatc
    15001 aataactcta aagcttcttt gactgttacc gtccgttcca cttttttctt cggtgtcaaa
    15061 gcacctagag tttcatttag gtcgatcccc atttgctcca atccattgtt catcattggt
    15121 gcaggtttct tttggtcttc aacttcaatg gttacttcac gactatccag taaacccttt
    15181 tgtagttgtt ccaagactgt acggcgattg attcgaatat cttctgtcac ttcttcttgc
    15241 ggttcagcgg tattttgctg actagcatta aaaagattca tcatttgttc aaattgatta
    15301 gtggattgtt tctgttcttt tttgatacca ggtaccaaga tttttaccaa acgtttttct
    15361 gcattttttt tagcacttgt gtatacacgg ctgtactgct ctttttctac gatttggatc
    15421 gcattttcta ctaaatcccg aaccattgat tctacatctc ttccgacata accgacttca
    15481 gtaaattttg ttgcttcgac tttgacaaat ggtgcattga cgatttttgc caaacgtcgt
    15541 gcgatttctg ttttaccgac ccctgtaggt ccgatcatca gcatattttt tggtgtaata
    15601 tcttgttgca ttttttcatc taactgcaaa cggcgatagc ggttacgcaa agcaacggca
    15661 acagattttt ttgctgcgtg ctgtcctacg atatattcat ctaattcact tacgatttgt
    15721 cttggtgttt gttttaattc atacatatct actcacctct tacagttctt ctacgataat
    15781 attatgatta gtaaagatac agatatctgc ggcaatattt aacgcatttt ctgcaatttc
    15841 tttcgcactc atgtcgtttt gagcatagtt tttcattgct cgggctgcag ccaacgcaaa
    15901 attccctcct gatccgatag ctaagatacc atcatctggt gtgataactt ctcctgtacc
    15961 tgaaacaaga agcatttctt tatcattcat tacgatcagc atcgcttcaa gtttttgcat
    16021 agattgctgt gtccgccatt cttgagctaa ttcgacagct gcgcgttgta gattcccgtt
    16081 atattcgttt agtttgcctt caaatttttc ttctaaagta aacgcatctg caacacttcc
    16141 ggcgaaacct acaacaactt ctccattgta gatacgtcgt actttctttg cagtgccttt
    16201 catgacaaca gattcaccca ttgttacttg gccatctcca gccatagcaa atttcccgtc
    16261 tttttcgacg gcacagatag tggttgaatg aaatggtgat tcgaccatgt ttgtttcctc
    16321 ctaaatttgt tcttttcctt atttttacgc tcttggatgg aaagaacgat agtttttttg
    16381 taaactttct tttgttacat gggcatagat ctgggtagtc gataaattgg catgtcctag
    16441 caattcttgg actgtccgca aatctgcccc attatttaat aaatgagtag caaatgtatg
    16501 gcgcagcatg tgaggatgga tatcactatc caaactgctt tttttgatca tttgattcaa
    16561 gacatattca attccagtag gcgtgattgg ttttccatga tgattcacaa aaacgatatc
    16621 atgctcttcg tgatattttg tcatcaatac ctggcggcct tccctgaaat attcttgcag
    16681 agcatcttga gcaaaagaac ctagaggagc atatctctct ttgttccctt taccatggat
    16741 caacatgacc tctgaatcaa aatcaatgtc tgataccgac aaactgctac actcacttaa
    16801 acggatgcct gacccgtaaa tgacttccag taaagcgcgg ttcctcaaat ccaaaggact
    16861 atctcctgct acgctgtcaa aaagtgcttg catttcattt tcataaaaaa aacgtggcag
    16921 tctcaaattt ttctttttga gatgaacgta agaaaatgga ttttctttta tcacttcttc
    16981 tttgagaaga tactgataaa acgaacgtag gctggctatc ttgcgactga tagaatttct
    17041 gctatagcta ggacgatgat cagctaatgc actcaaatag acacgaaccg taaaatattc
    17101 cgccttcagc agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat ggagaatgtc
    17161 ttcttcatat gcttctttcg ttttttctga atatcctctt tcgacaatca aataacgcaa
    17221 aaatttttgt tcccacgtgg cttctgtcat tttctcacct tccttttgtc ccagagaata
    17281 atttagcaca gcgtttataa aaagacaaat aaattgtcag aatttaacca atatttctaa
    17341 gtttgttcac aattggcgca attcctcaca ttttgtttga gaaattctta agaaaaacgc
    17401 aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg agtttcctta ctcatttggg
    17461 attgcagttt tttctaattc tgtttttatt gcctcattag cttttaaagc tcgttgtgca
    17521 attgcttcat atcttgcttt tttgtctcga atgcgctctg gcagttcagg aaacagacca
    17581 tagttggcat tcatcggttg gaaatgtttt ccttccgcat gagtaatgta gtaagccata
    17641 cttccaatag ctgtttcttg tggaaactcg actggttcca tttcttttgc taagcgtgcg
    17701 gcgttgattc cagcaagtag tccgctcgct gcactttcaa catagccttc tacaccagtc
    17761 atctgacctg caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg tttcagcaat
    17821 tctggcgaat tcataaagct gtttcggtgc atcacaccat agcgaacgaa ttcagcattt
    17881 tctaacccag gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa gtgagtctga
    17941 aagcccacga tgttgtataa agaagctgca gcattgtctt gacgcagctg gataacagca
    18001 taaggacgtt tgccagtttt aggatcttcc aatccaactg gtttcatagg tccgaaaagc
    18061 atcgttttga atcctcgctg tgccattact tcaattggca tacatccttc aaaatatttt
    18121 tctttctcaa aagatttaag ttctgccact tcggcattga ttagtgcctc atggaaagct
    18181 ttgaattcct cttctgtcat cggacagttc aagtaagctg cttctccttt gtcatagcgg
    18241 gacttcaaat atactttatc catatcgatc gtagctttgt ctacgattgg tgcagctgca
    18301 tcatagaaat aaaaaccatc tgaaccattg aatgctttga tgctttctgc taatggatga
    18361 gaagtcaaag gtcctgttgc tactacggtg attccttccg gtatttcagt aatttcttcg
    18421 tttatcactg ttactaatgg atgctctttg acttttcggg taattgtttc agaaaaagaa
    18481 tcgcggtcaa cagctaaggc accaccagct ggcactgctg tttcatctgc cgaagtgatt
    18541 actaccgaat tcaatcggcg catttcttct ttcaataacc ctactgcatt agccaaactg
    18601 tttcctctta atgaattgga acatactagt tcagcaaaat tacttgtgtg atgcgcctca
    18661 gtcgatttga ctggacgcat ttcatataaa cggacaggga ctcctgcttg tgcgatctgc
    18721 caagcagctt cacttccggc aagccctgct ccaacaacat ttaccatttt caatgaaaga
    18781 acctcttttc tattattttt ggatgtcttc ttcgtaatct ccatttacac agactacttg
    18841 tttaccgcct ttgactttct tctcgaccag atatccgtca cattttggac aatttctgcc
    18901 cactggttta tcccaagaag tgaattcaca ttctgggaaa cgggaacaac catagaatag
    18961 tctgtttttc ttagattttc gttcaatgac ttgtccttca tgacatactg ggcaagtcac
    19021 accgatttct tttacgattg gtttcgtatt ccgacaatca ggaaaattac tgcaagcata
    19081 aaacttccca tatttcccta attttatcac catcggatgt ccacagacat cacaatcgaa
    19141 tcctgctggt tcatctttga tttgaatttt ttcgattttt tcttccgcat tcgtcagttc
    19201 tttttcaaac ggtttataga aacgatcaac cactttgacc cacgcttccg tgccttcttc
    19261 aattttatcc aaatctcctt ccatttctgc tgtgaaatgg atatcgacga tttgagggaa
    19321 gaattcacag attaatgaat tgacaatttc tcctagttca gtcggctcaa atcttttttg
    19381 tgtcaatttt acatagtagc gtctttggat cgtatcaagt gttggtgcat aagtggaagg
    19441 tcgccctacc ccgttttctt ctagtgcacg aatcaacgta gcctcactaa aacgtgctgg
    19501 tggttgagta aaatgttgtt ttggttcaat atcaacagat tggactacat cgccttcttc
    19561 aagttctgga agaatatttt ctttctcttc tttgccgtca tctctacctt ccacatacac
    19621 ttgcatgaat cctttgaatt tgatttttga accgttcgca atgaagatga caccattttg
    19681 ttctaatgtc actttcattg tatctaaaat agctggtgtc atctgactag ccacaaaacg
    19741 tgaccagatc aatgtataaa gtttcaattg atctttatct aaatattgtt tgagttcatc
    19801 tggtgtacga agcacactcg ttggacgcac agcttcatgg gcatcttgtg cgccttgagc
    19861 atttttgatc tttcggtgat catgtctaga aaattctttt ccatacgtat cttcaataaa
    19921 tgctgcggcc tctgcttttg ctgtatcggc aatccgggta gaatcagtac gcatgtatgt
    19981 aatcaagccg actgttcctt gtttacccaa agtgattcct tcatataatt gctgtgctac
    20041 catcatggtt tttcttgttc gaaagtttaa cttacgggca gcttcttgtt gcaggctact
    20101 tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc ttttctactt ttttaacttc
    20161 gaagtctgtt ccatcaattc tagcagtgac ttcttttaca ctttgtgcat ctggtaattt
    20221 ctttttctta ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag cttttttaaa
    20281 attaccgtcg atgctccaat attcttctgg aacaaattct cggatctctt tttcacggtc
    20341 aataataatt tttaaagcga cagattggac acgtcctgca cttaaccctt tcttgacctt
    20401 acgccagaga ataggactga tcgaataacc aacgattctg tctaaggtac gacgagcttg
    20461 ctgtgcatct actaaatcta catcgatcgt tcttggttcc ttaaaagctg ctttgactgc
    20521 gtcttttgtg atttcattga agaccacccg atttttttct ttcagatcca acccaagtag
    20581 gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg ccaagtaaac
    20641 tttttgagct ttttttgctg cggctttcag gcttttgatg acatcgcctt ttccgcgtat
    20701 agaaatataa tggggttcat aattgttttc aaaatcgata cccattttac ttttcggtaa
    20761 atctcttata tggccaacac tggccattac tttatagttt cgtccaagat atttctcaat
    20821 tgtcttggct tttgcaggtg attctacaat cactaaatat ttatacgcca ttactgaatg
    20881 gactcctttc cattgtcgtg ttcctactat taaaagagga ttcatacaaa tcgtagctaa
    20941 tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg tgttattctt
    21001 taaattctgc caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct tggatcaaat
    21061 gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa actgttcttc
    21121 caagatccaa tgcttgctgt gctgttatca gagagccgct ttttttcttg gcttcaaaga
    21181 cacatgttcc tttagacaaa gctgcgataa tccgatttct cattggaaaa caatgctttt
    21241 tcgggccgga ttgaggagga tattcactca aaagaagatg atcatttgca atcacctctt
    21301 gcaagtcacg attttcagca ggatagactt gctctatccc tgttccaatg actgcgattg
    21361 tccgtccacc atgagagatt gctgcttggt gaccgtatgt atctatccct ttcgcgcagc
    21421 cactgacgat cacatatcca gcttctatca ctggttttag taaataataa actgtttttt
    21481 tggctaaagc agaagtgtgt cttccaccaa caacgcttaa catgttacaa gaaagtaaag
    21541 atagatcacc attataaaac agtacaattg gcggataggt caagtgaagc agctgagggg
    21601 gatatttggg atcaaagcaa gtgataaacg actgtttcga ttggattttg ccccagttag
    21661 cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg tttggttatt ttacctatct
    21721 taatgatgtc ttcaaatccg atttctttat aaggatactg catcatccag ttagcaagtg
    21781 cccattttcc ttgactactg attccttttg tataagcaag tttcaataaa ttttcttcta
    21841 tttgatacat cataaaaccg cctttcgtct ctctttctaa taagatacgt aaaagcggct
    21901 gttttttatt gtaaatattt ttttacaggt gcaaaacttt tacgatggat tggcgtaatt
    21961 ccttgtcgtt caagtccttc taagtgttcc tttgttccat aacctgcatt atgcgagaaa
    22021 ccatatccag gataaagcac gtcatattct ttcatcaatt ggtctcgata tactttagca
    22081 atgatacttg cagcagcaat cgacacagat cgtgcatccc ctttgattat tttttcctgc
    22141 tgaataggca aatccaaggt catcgcatcg atcaacaaat agcttggtac aatggacaat
    22201 ttttctactg cttggatcat cgctaattta cttgcttcat aaatattcac atcatcgatt
    22261 gtctgctgat caacgatccc gatcccgatt gccagggctt tttcttgaac ttgttgataa
    22321 agaagttctc gctttttctc tgataattgt ttggagtcat tcagccctaa tatgatctca
    22381 tttttcggca agataactgc agctgccacg acaggtccag ctaatggtcc tcttcctact
    22441 tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc aaagcaaaac
    22501 atttcttcat atttttcctt aattaatgct tcctttttca atcgtcgttc aaaagaagcc
    22561 agtgcttgct gcacgccttt gcgtggatca tttcttaatg attctagtgt aggatcatct
    22621 attctctgta tgcttttcaa tcgttctttg atactttgga tggattcagt catcactttt
    22681 ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca agttttcctt gacgaatatc
    22741 atgcacgact aattcgctgc cacgatcata gtcttcttta tacccacgtt ttgctgtaat
    22801 atccatcagt aattccggtg ccaatttttc ttcttgctcg attgataaac cataacgatc
    22861 gctgattctt cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg ctaaatcatc
    22921 taaatggaca agctggtctt tgatcgcgcc tgtcagcgct agttttttgc cgatttcttc
    22981 atcttcaaat ttgggccata aaatccctgg tgtgtctaac aattccaact cattacctaa
    23041 acgaagccac tgctgacctt ttgttacgcc aggcttattt cctgtctgtg caattttctt
    23101 gcctactagt cgattcatca acgtagattt cccaacattt ggaataccta agaccattgc
    23161 ccgaatagcg cgaggcttga tcccccgagc agcatctctt gcccgttttt ctttcaatgc
    23221 ttctttagcc gcaggtacta gacctttaat tcctttactt tcttgtgccg tgatagctaa
    23281 tgcaagatgg ccttgtttac gaaaatattc ttgccacagc tttgtctgtt gtggatctgc
    23341 cagatccgct ttattcaata aaacaaggcg aggtttttgc tgaatgatct gatctagcat
    23401 tggattacga gaagataaag gtaatcgtgc gtctaccaat tcaaatacga tgtccacata
    23461 tttcattttt tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa accattggat
    23521 ggtcattgtt ctctccattc ctttcatacc ggaatattcc ccggctttac ttgttttttt
    23581 ctttttcaat ataaattcac ttttttatta taaagaaatt ccatctatta gttaagtgaa
    23641 agtgaaccag tcctcattct atcacatctt cacttttttg atgatgcaaa tttttatttt
    23701 ggtttctata tatgaatcta aatttttgac agcgtttcct aaataagaga tgatagacga
    23761 acaaaacaga aaggagacga aacaaatgaa tagtatggtt tttgttaatt ttcctgttgc
    23821 tgatgtcaat cgttccgttg ctttttatga aaagctgggt tttaaaaaaa atgaagaatt
    23881 ttctactgaa gaagctggtt caatggtatg ggatgacact ttttggataa tgcttctctc
    23941 tcatgatttt tataaaaaat ttataaaaga taaagaaata gctgataata aaaaaacaag
    24001 tggtgctttg atttcgttta gtgttgaaaa tgcggatgaa gtaaaaaaat ttgctcaaat
    24061 tgcaaaagaa aatggtggta ctttccacga agtagatatg ggaatgcctg aagaagaaat
    24121 gttttcatta gaagtccaag atctagacgg aaatacatta gaacccgtat ggatgaagat
    24181 gtaagcagat aactaatctg acgtttttta taaaaaaaaa acaaaggcgg atgatacgat
    24241 aagtattctc cgcctctatt ttttttattc gtacaatctc ttttttacag tttataaaag
    24301 cagtcttctc accctttttt taattgttag atgccagtgt atgtaaatca ccaatgtgtc
    24361 atgacacatt ggtgatttta tggtatcctt ttattattaa ctgacaaagg agagaatatt
    24421 ttgaacactt tgattgcaaa agacgttttt agattgcccg aaatgaatgc aagattgttg
    24481 tcgacctctg ctcttttatt agcccttgat gtcatcttat acaagatagc gattggtcct
    24541 gcttattttc aaatcacatt cggattttta agtatggcgc ttatgggtta tctatatgga
    24601 ccgatctggg ctggtttgct tgaaatgttg tcgaacactt taaatttcac gatatttggt
    24661 tcaggaacgt ttcaagctgc ttttcttatc acggctttcc ttggtggatt tattaatggc
    24721 ttatttttat atcataaaaa gataaacctg ccttccgtca tcgctgccca attattgatt
    24781 atgatcccag tcagcctttt tatcaattct tggctgatgt ctgttttgtt tggtgcagat
    24841 ttcaaagtaa tttttctagg tagattcatc aggaatatga tccaaatccc tattcaaata
    24901 atggttgttt attcatttat caaaacttta gaggctagaa aagtgtttaa acgagctgtt
    24961 tgattttacg tactaaggta gtaaactgat tttggattaa acaataaagc gtgcagtctc
    25021 aacagactgc acgctttatt gtttctcact ggatccattt tatttcatca agtggataat
    25081 agacaaatct tgctttacct agaatatcag ttttattgat cgtgccaaac gaacgactat
    25141 cttttgaaac tcttcgatta tctccaagta caaaataatt atcttccgga agcttggatt
    25201 gtcccgttag ctcttgtagt gtaaagtccg tcgtatagga tgcatttgca tgatctgatt
    25261 tcaggttttt agttagataa ggttccttaa ctactttccc attgatcttc aattgatcat
    25321 tttggtagct gacattttct cctggaagac cgattactcg tttgatataa atcgtgccat
    25381 ctgctaattg aaaaacaaca atatcaaatc gtctgatctc cgaaaatttt tccatcacaa
    25441 ccatatcacc ttgcttcaac acgttttcca ttgaattgcc ttccactggt accgggatga
    25501 ataaaaatcc ccgcaacatg aacgcaagga atacagaaac aagtaagtat ttgaatacaa
    25561 gccagaagcg attaatatag cgttgttttt ttgtcaatct ctatcaatcc atccctcttt
    25621 ttttcattct ttctttatta taagaaaaat atcaaaaaaa tgcgactaga tagctgaaat
    25681 aaaaataatt ctttcaatta ggctattgga caaaaaactt ttttgatcat aaagtacaaa
    25741 actatctctt atatgtcatg attattttct aaatattttt cccatgcttc gtcaaagata
    25801 tccatggacg gcaaatagtc gacattcagt tccagataat tagaaatttc ttcgtagcta
    25861 gagctgtgct taggaaattg gatgtcttta ccggcttctg tagcgaaaac agataaagca
    25921 tcatgtgtgc caccgcctcg cagtgtcatt aaataatgaa aaaagcttcg attcaccctt
    25981 cgttcctcca attttcttcg ataaaggcag cccggcgtgc atggtttctt ttaaaatttt
    26041 caggatgctt tttataatac tcttgatggt cttcttctgc tagataaaat ggttgggctg
    26101 gttcgatttt tgtaacgatt ggctgttcga aacgtccact ttcagctaac tgtttcttac
    26161 ttttttcagc aatgatccgc tgttcttctg tcaaataaaa gatcaccggg cgataattgt
    26221 caccgcgatc ctcgaattgt ccgaaagcgt cagttggatc agtttgctgc cagtaaagtt
    26281 ctactaattg ttcatatgaa atcaatgttg gatcatagat aatctccact gcttctgtat
    26341 gtccagtcgt ttggcttttt acttgttcat aagtcggatc tggtacatgc cctccagtat
    26401 atcctgacat cactgtatgg ataccaggaa gcgtgtcgaa aggctggatc atacaccaga
    26461 agcagcctcc tgcaaatatt gctcgttcca ttatgattcc ccctctaata ctactcatcc
    26521 gatgattttt tattttcttt tgtcttgctt tcagtactgc tggtgctagt tttgttggta
    26581 tttgagttgt ttgaagcatc agaggtatct tttggcaaat atagactaaa ccgaatgtca
    26641 tcatttacta agtcgatttt atctgcttta atatacatcc cgttttgcat tcggaattga
    26701 tccaacctaa gcatgatagt cagatcatct gtattgactt cgatccaatc tggtaattta
    26761 tagtttctct tgatcatatt catcacatct ttgattggca aactcaatgt tccgatagaa
    26821 agacttctag cttttagttg gacatttccg ttttccatca catacggatc aaaatataaa
    26881 tagaaatcga taggaaaacc tagtact
//
LOCUS       DAXWLH010000022        25627 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXWLH010000022 DAXWLH010000000
VERSION     DAXWLH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.022"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..252
                     /locus_tag="ACGE36_000667"
     CDS             <1..252
                     /locus_tag="ACGE36_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFF8383087.1"
                     /translation="DNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRSSID
                     RVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            266..580
                     /locus_tag="ACGE36_000668"
     CDS             266..580
                     /locus_tag="ACGE36_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFF8383088.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(1310..1864)
                     /locus_tag="ACGE36_000669"
     CDS             complement(1310..1864)
                     /locus_tag="ACGE36_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFF8383089.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1990..3919)
                     /locus_tag="ACGE36_000670"
                     /pseudo
     CDS             complement(1990..3919)
                     /locus_tag="ACGE36_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(4225..4941)
                     /locus_tag="ACGE36_000671"
     CDS             complement(4225..4941)
                     /locus_tag="ACGE36_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8383090.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4942..6765)
                     /gene="glpO"
                     /locus_tag="ACGE36_000672"
     CDS             complement(4942..6765)
                     /gene="glpO"
                     /locus_tag="ACGE36_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFF8383091.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(6779..8275)
                     /gene="glpK"
                     /locus_tag="ACGE36_000673"
     CDS             complement(6779..8275)
                     /gene="glpK"
                     /locus_tag="ACGE36_000673"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFF8383092.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(8411..9871)
                     /locus_tag="ACGE36_000674"
     CDS             complement(8411..9871)
                     /locus_tag="ACGE36_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383093.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9861..11156)
                     /locus_tag="ACGE36_000675"
     CDS             complement(9861..11156)
                     /locus_tag="ACGE36_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8383094.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(11488..12165)
                     /locus_tag="ACGE36_000676"
     CDS             complement(11488..12165)
                     /locus_tag="ACGE36_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFF8383095.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(12195..13286)
                     /locus_tag="ACGE36_000677"
     CDS             complement(12195..13286)
                     /locus_tag="ACGE36_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8383096.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(13387..13821)
                     /locus_tag="ACGE36_000678"
     CDS             complement(13387..13821)
                     /locus_tag="ACGE36_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8383097.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(14405..15862)
                     /locus_tag="ACGE36_000679"
     CDS             complement(14405..15862)
                     /locus_tag="ACGE36_000679"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8383098.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15859..17754)
                     /locus_tag="ACGE36_000680"
     CDS             complement(15859..17754)
                     /locus_tag="ACGE36_000680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8383099.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17812..19587)
                     /locus_tag="ACGE36_000681"
     CDS             complement(17812..19587)
                     /locus_tag="ACGE36_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8383100.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(19637..21133)
                     /locus_tag="ACGE36_000682"
     CDS             complement(19637..21133)
                     /locus_tag="ACGE36_000682"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8383101.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(21327..22949)
                     /locus_tag="ACGE36_000683"
     CDS             complement(21327..22949)
                     /locus_tag="ACGE36_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8383102.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(23277..24590)
                     /locus_tag="ACGE36_000684"
     CDS             complement(23277..24590)
                     /locus_tag="ACGE36_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8383103.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24835..25059)
                     /locus_tag="ACGE36_000685"
     CDS             complement(24835..25059)
                     /locus_tag="ACGE36_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383104.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(25079..25501)
                     /locus_tag="ACGE36_000686"
     CDS             complement(25079..25501)
                     /locus_tag="ACGE36_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8383105.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT         7409 a         5541 c         4239 g         8438 t
ORIGIN      
        1 gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa
       61 aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt
      121 tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag
      181 aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt
      241 attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa
      301 atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat
      361 tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt
      421 cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat
      481 ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt
      541 tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga
      601 agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt
      661 gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
      721 agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      781 gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga
      841 aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca
      901 ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt
      961 gaaaatggat ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
     1021 ataccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa
     1081 cggaaaaata gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt
     1141 gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat
     1201 ttcaaaaaca acttctcctg tttttggttc ggttggctta ttatcgagaa gtagctactg
     1261 ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt
     1321 gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga
     1381 tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga
     1441 tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt
     1501 tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga
     1561 atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa
     1621 gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat
     1681 atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc
     1741 ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac
     1801 ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt
     1861 tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa
     1921 taaaaccaat gatttcactg tttttatttt tctttacaga cactgatatt atgagctaat
     1981 ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat
     2041 ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt
     2101 acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc
     2161 tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata
     2221 tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt
     2281 gacaaatatc cgatcacctt ttgcaaagag ctgacgttct tttttattct ccttcctatt
     2341 tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa
     2401 gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa
     2461 agcttggctt tctcctgttt cttccaaccc gcaactgata tttaggacga agtgcttccc
     2521 gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat ttcttgattt
     2581 ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat tccatgattg
     2641 cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca tctaaaagta
     2701 ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata tgaccagaaa
     2761 atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa atctcatcaa
     2821 agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc gccatcaaag
     2881 tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct ccggtgataa
     2941 tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc tcaggtaaaa
     3001 atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta ggtgtgcttc
     3061 cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc gtgatcgttt
     3121 cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat gccgctagga
     3181 cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca ttcaatttag
     3241 aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg ataatcgttc
     3301 catctatttt atttttatag ctggctttga taggaatcgt atagtattcg cctttttgaa
     3361 caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat ttcagtaatt
     3421 ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc aaattacgtg
     3481 acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct tctacattta
     3541 tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat tgattcttat
     3601 caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg atcaaatcgg
     3661 ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc ctcggcaacc
     3721 ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt gtttcttctt
     3781 gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt cgttttttgc
     3841 tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat tgtaattgac
     3901 taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc ccaaaataaa
     3961 taagggccta gagataaata aaaggataga acacagctta aaaaagtcat gtcctatcct
     4021 tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca caccaaataa
     4081 actttgttta ttggctattc gacttactga ttagatacca gctacactca caaaacatac
     4141 cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca ttttggttgt
     4201 caaactttag tatgttttat agatttacac cattaatgaa tagacaccta ccgcgataat
     4261 tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg aagttccttt
     4321 attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag cagggttgat
     4381 tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc cacctacagc
     4441 gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc caagaactaa
     4501 tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta tagcaggacc
     4561 tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat gtggcaaata
     4621 agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa tgatgaaagg
     4681 aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga ctgcaggatt
     4741 caaatgagct gggcccatga atccagaaat atagacagcc attgttacag cgagtcccca
     4801 accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta aattcacagc
     4861 cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact cccccatgat
     4921 ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat ctgattctgc
     4981 aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc attcaaaata
     5041 tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc gttcaaacaa
     5101 cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt cttcttctaa
     5161 accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg ccatttcttt
     5221 tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat aagtagcatc
     5281 ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg tttcttctaa
     5341 tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga tttctttcgt
     5401 ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc cttctgccat
     5461 cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg gttctctttc
     5521 taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt tttccaaatg
     5581 gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt cacttactgt
     5641 atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac cattgtaatc
     5701 agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt cgatatcatt
     5761 caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat aatctacatc
     5821 ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg tcgtaccaaa
     5881 gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct ttcctgtatc
     5941 gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat ggatcccttt
     6001 agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta ctttatctac
     6061 ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt catctgtcag
     6121 caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc caaccgcttt
     6181 cattttactt actagataag ccccatcttc ggcagccttt ttgatattat caatcactaa
     6241 acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc cttctttttt
     6301 caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct catatttggt
     6361 tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg agaacatatt
     6421 gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg gttttgggat
     6481 gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta ctacttccac
     6541 atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc gagaggaagt
     6601 cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg ctgctgactg
     6661 gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca attcttcttg
     6721 ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga tttcctcctt
     6781 attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt ttttctcggc
     6841 gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg cggatctcgt
     6901 ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct agcgccgttg
     6961 tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg aattgcatca
     7021 gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg attccagtgt
     7081 catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt aaagtagctt
     7141 taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct cgtgctgcct
     7201 gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc tcatcttcgc
     7261 ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt tgaagtccat
     7321 ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa gcatagtaaa
     7381 cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat aattgcggtt
     7441 tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg accatgcctg
     7501 gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc cctgaaattg
     7561 gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct gagttagaag
     7621 tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc tgatcccaat
     7681 ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca gtaacatgtg
     7741 cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac atcaattcgc
     7801 ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt atcttcgttg
     7861 ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct tgatagcctt
     7921 cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca actgcatgat
     7981 agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg ttcgttatgc
     8041 caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg gcaataactg
     8101 actgtacaga attccagatt tcatttgcat catgttccac ccaactttct tgcgggaaat
     8161 attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca aagataattg
     8221 ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct gtcattgtga
     8281 ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac tgttttaaaa
     8341 aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt attatgaagc
     8401 tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc aatcgaataa
     8461 tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg tttctctggc
     8521 aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc tcaataccgc
     8581 cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa atcggcagac
     8641 tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc atcaaaagaa
     8701 cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt ttcctcggca
     8761 attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa tgcccgagca
     8821 ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt ccaacaaaga
     8881 aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct tctttgattt
     8941 tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta tctaaaacag
     9001 cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct tcgtctacta
     9061 tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc agttctgctg
     9121 ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc cggtccgact
     9181 gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc aatagtcttt
     9241 ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta aacgttgttt
     9301 ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt tgcaaaaaaa
     9361 gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat cgtatctgta
     9421 gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct aagagctggt
     9481 tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct gtcgctaatc
     9541 gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac ttcaagcttt
     9601 cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac acaagcagcg
     9661 cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca atcaattcaa
     9721 tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct ctcaaaccat
     9781 tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc tgtaactgct
     9841 cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt tgaccacacc
     9901 atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa ctgttcaaac
     9961 gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat ctcttttttg
    10021 ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt gagtttaact
    10081 ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat tttttgggga
    10141 taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc aaaccactga
    10201 ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat tgtcttgctg
    10261 acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt ttcatttgtt
    10321 ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg agacgtttgc
    10381 aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc ttcccacatc
    10441 tgattgtcgg gacgagaatg attagccagc aaaacaagat catagttctc ctttcttttt
    10501 tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc ttctaaaaaa
    10561 agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc aggatctaga
    10621 taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt tcttgatgcc
    10681 aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat tttctttgca
    10741 ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg gtgaataagc
    10801 gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag aatcaaacga
    10861 tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa tcctattact
    10921 gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt cgtgatccaa
    10981 gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc gctgggaatc
    11041 catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat caattggcga
    11101 agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat gttcatctgc
    11161 attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac acagtattca
    11221 taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct ctccatcata
    11281 ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag agtatcattt
    11341 ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc ttttacgtac
    11401 ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg tgtaatgtaa
    11461 tagttagaat agacacatca tttattttta attctttaat gcttcaacaa ctgcttcgcc
    11521 taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa tttgaaccac
    11581 ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag catcttctgc
    11641 taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac tgaacccagt
    11701 cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca acaatcctac
    11761 aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg aagcgacttt
    11821 cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag cataataaac
    11881 cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca aggcccgatt
    11941 tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca aaacactttg
    12001 cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga tcccattttt
    12061 tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag gacgctcgat
    12121 cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc catcctcttt
    12181 ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg gcttggcaaa
    12241 taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc acggttttcc
    12301 aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc tgcaagttct
    12361 gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac accaactaaa
    12421 gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc gttgtattta
    12481 tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc tagttttgac
    12541 tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg tgtttcatat
    12601 tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg ttcaaccgct
    12661 gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa gctaccgccc
    12721 cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa atacgtattt
    12781 gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt ggctttgcca
    12841 aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt ttccgcatca
    12901 ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa agttgctcga
    12961 cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc tccttggatt
    13021 gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct gagttcacct
    13081 ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc accaagacct
    13141 ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt gaaaaagctg
    13201 agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc tgaaggtaaa
    13261 gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc tgattaacct
    13321 atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta atttgcttaa
    13381 aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt
    13441 acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc
    13501 gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc
    13561 gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga
    13621 tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa
    13681 tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc
    13741 taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc
    13801 tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt
    13861 ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa
    13921 gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg
    13981 tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa
    14041 tggctttcca catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc
    14101 gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa
    14161 aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca
    14221 ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa
    14281 aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa
    14341 tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct
    14401 tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt
    14461 tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat
    14521 aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat
    14581 aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata tgctgcttca
    14641 aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc
    14701 cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag
    14761 caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga agatacggat
    14821 ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac
    14881 tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga
    14941 tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg
    15001 tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct
    15061 gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc
    15121 tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag
    15181 cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt
    15241 tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga
    15301 aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa
    15361 cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg
    15421 gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa
    15481 acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa
    15541 aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac
    15601 ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact
    15661 tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg
    15721 tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt
    15781 tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa
    15841 atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt
    15901 atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat
    15961 tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc
    16021 gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc catttaaatt
    16081 cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat
    16141 tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt agatttttcc
    16201 gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt
    16261 tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga
    16321 ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat
    16381 catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa
    16441 agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc
    16501 accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac
    16561 accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa
    16621 actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc
    16681 agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt
    16741 ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc
    16801 gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc
    16861 aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa ttgggaccat
    16921 cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg
    16981 aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact
    17041 tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata
    17101 tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc
    17161 tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc
    17221 atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact
    17281 cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg
    17341 ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc
    17401 tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac
    17461 ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat
    17521 cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa
    17581 gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg
    17641 gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc
    17701 cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt
    17761 agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag
    17821 caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac
    17881 aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa gctgatactc
    17941 tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct cggcatttaa
    18001 cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat ctatcttttt
    18061 atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg ttttcttttt
    18121 ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag attcaaactc
    18181 atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct cttgctcagt
    18241 agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa gatacactgg
    18301 ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt tttttagtgt
    18361 attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt ttcgaaaagg
    18421 attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa
    18481 ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat aacgggagat
    18541 gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg ttttcatttg
    18601 tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag gaagatctaa
    18661 aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt tgtttttttc
    18721 atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt gtttttcttg
    18781 attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct ctaccatcgg
    18841 aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa gaatggtcaa
    18901 aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat tatatgatat
    18961 aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa ttgtactgat
    19021 ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag ataagaaaat
    19081 tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa cttccagttc
    19141 taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg atacggctgt
    19201 ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt tttgaaacaa
    19261 taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt tatgtttcgt
    19321 cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa aataacctga
    19381 acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat ttttgatttc
    19441 agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc ttgttatcca
    19501 atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta agtccatttg
    19561 cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac aatagtcact
    19621 tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta ccattagact
    19681 ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag cttccgctcc
    19741 catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc cctgttcctg
    19801 ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa ccatctttta
    19861 ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt ggatcgtgga
    19921 ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact atgaataatg
    19981 gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga ggatcaattg
    20041 gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa ccatttgctc
    20101 cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga taataactaa
    20161 atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct aacattactg
    20221 gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc cctcggcaca
    20281 tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat acattctctg
    20341 gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata cccatcttag
    20401 catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga aacatatgat
    20461 gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt ttatctctag
    20521 cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta ttgatctcat
    20581 taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc tgcgcataaa
    20641 attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct accaatgcta
    20701 agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga tttaatgtat
    20761 tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg tctcctcttg
    20821 gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac tccttcagta
    20881 aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa agataatgga
    20941 gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga atactaggat
    21001 gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg tcttccaacc
    21061 atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt tctggaaata
    21121 tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga agtatagctc
    21181 gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa aattgaatgc
    21241 tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc gaaagaaaac
    21301 actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc ttcataaggt
    21361 cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac aataaccctt
    21421 aaagcttcac gtggaatagt gaacgtctgc ttttcactgg acatattaca aataacgagc
    21481 cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt cttttccact
    21541 aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat caatttcttg
    21601 tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat ttctttataa
    21661 tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc acgggcgttc
    21721 cattgcatag gcgtcctagc attgtctctt cctttcgcct tgattgattc caagatttcc
    21781 tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc aatatctttt
    21841 gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc ttgatagata
    21901 taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact ttcttttcga
    21961 tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt attccaaaat
    22021 aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac ttttttcagt
    22081 tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg atctaatcct
    22141 atgtgttcga attggaagac catggataat tcatttcttt taggattgga ataaagttta
    22201 gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa
    22261 gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact tgtgatttct
    22321 ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc aataccttta
    22381 tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt tttccaattc
    22441 aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt ttcatccaac
    22501 tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc attgaccgga
    22561 tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc ctcaatgaac
    22621 cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg gatatttctt
    22681 ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc tgccatgatt
    22741 gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta tactggagaa
    22801 agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat aatcccccgc
    22861 aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat ttgataaaca
    22921 actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga tgtttctatt
    22981 ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt tcaaatagct
    23041 attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa attccctcaa
    23101 ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa cttctttata
    23161 aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa tataagaaac
    23221 tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat
    23281 ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata catgattgga
    23341 gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat
    23401 gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg
    23461 atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct
    23521 accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag
    23581 aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca
    23641 acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa
    23701 cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg
    23761 tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa
    23821 gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca
    23881 tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa
    23941 ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat cccgatgaag
    24001 atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc
    24061 aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg
    24121 atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc
    24181 gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg
    24241 atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc
    24301 cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt
    24361 ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca
    24421 gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca
    24481 aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc
    24541 gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc
    24601 cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg
    24661 tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa
    24721 tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga
    24781 ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata
    24841 gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac
    24901 aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt
    24961 cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg
    25021 atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc
    25081 aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga
    25141 aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag
    25201 tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa
    25261 acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta
    25321 caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt
    25381 ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt
    25441 gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca
    25501 ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata
    25561 gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg caatgaatgc
    25621 cgctctt
//
LOCUS       DAXWLH010000023        24809 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXWLH010000023 DAXWLH010000000
VERSION     DAXWLH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.023"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            45..1943
                     /locus_tag="ACGE36_000687"
     CDS             45..1943
                     /locus_tag="ACGE36_000687"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFF8383106.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2242..2961)
                     /locus_tag="ACGE36_000688"
     CDS             complement(2242..2961)
                     /locus_tag="ACGE36_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFF8383107.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3151..3198
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3298..3630
                     /locus_tag="ACGE36_000689"
     CDS             3298..3630
                     /locus_tag="ACGE36_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383108.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3617..5620
                     /locus_tag="ACGE36_000690"
     CDS             3617..5620
                     /locus_tag="ACGE36_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFF8383109.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5639..6109
                     /locus_tag="ACGE36_000691"
     CDS             5639..6109
                     /locus_tag="ACGE36_000691"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFF8383110.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6130..6714
                     /locus_tag="ACGE36_000692"
     CDS             6130..6714
                     /locus_tag="ACGE36_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFF8383111.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6729..7715
                     /locus_tag="ACGE36_000693"
     CDS             6729..7715
                     /locus_tag="ACGE36_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFF8383112.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7705..8016
                     /locus_tag="ACGE36_000694"
     CDS             7705..8016
                     /locus_tag="ACGE36_000694"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFF8383113.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8038..9819
                     /locus_tag="ACGE36_000695"
     CDS             8038..9819
                     /locus_tag="ACGE36_000695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFF8383114.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9812..11188
                     /locus_tag="ACGE36_000696"
     CDS             9812..11188
                     /locus_tag="ACGE36_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFF8383115.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11195..11830
                     /locus_tag="ACGE36_000697"
     CDS             11195..11830
                     /locus_tag="ACGE36_000697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFF8383116.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11860..13215
                     /locus_tag="ACGE36_000698"
     CDS             11860..13215
                     /locus_tag="ACGE36_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8383117.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13407..14801)
                     /locus_tag="ACGE36_000699"
     CDS             complement(13407..14801)
                     /locus_tag="ACGE36_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8383118.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14893..15864
                     /locus_tag="ACGE36_000700"
     CDS             14893..15864
                     /locus_tag="ACGE36_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8383119.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15861..16643
                     /locus_tag="ACGE36_000701"
     CDS             15861..16643
                     /locus_tag="ACGE36_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFF8383120.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16812..17183)
                     /locus_tag="ACGE36_000702"
     CDS             complement(16812..17183)
                     /locus_tag="ACGE36_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8383121.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17317..17760
                     /locus_tag="ACGE36_000703"
     CDS             17317..17760
                     /locus_tag="ACGE36_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8383122.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17803..18612)
                     /gene="yidA"
                     /locus_tag="ACGE36_000704"
     CDS             complement(17803..18612)
                     /gene="yidA"
                     /locus_tag="ACGE36_000704"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFF8383123.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18631..19998)
                     /locus_tag="ACGE36_000705"
     CDS             complement(18631..19998)
                     /locus_tag="ACGE36_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8383124.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19982..20812)
                     /locus_tag="ACGE36_000706"
     CDS             complement(19982..20812)
                     /locus_tag="ACGE36_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFF8383125.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20975..21412
                     /locus_tag="ACGE36_000707"
     CDS             20975..21412
                     /locus_tag="ACGE36_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFF8383126.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21488..22108
                     /gene="rpoE"
                     /locus_tag="ACGE36_000708"
     CDS             21488..22108
                     /gene="rpoE"
                     /locus_tag="ACGE36_000708"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFF8383127.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22297..22803
                     /locus_tag="ACGE36_000709"
     CDS             22297..22803
                     /locus_tag="ACGE36_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFF8383128.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22820..23719
                     /locus_tag="ACGE36_000710"
     CDS             22820..23719
                     /locus_tag="ACGE36_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFF8383129.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23806..24690
                     /locus_tag="ACGE36_000711"
     CDS             23806..24690
                     /locus_tag="ACGE36_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8383130.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT         8073 a         4337 c         5294 g         7105 t
ORIGIN      
        1 acaactttat aatgttgagt ataccaattt ttggaggcgt tttaatggac cgctatttag
       61 atttactttc agagaagttc cctacaacgg aagatgtcat cacggaaatc atcaatctcg
      121 aagcaatttt gcagcttcca aaaggaacag agttattttt gagcgatata catggcgaat
      181 ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag
      241 accttttctc tgaacaaatg acggaaacgg agatcagcca attcactatt tttacggctt
      301 atccagaata tgtgttgacg actgattggt acaaaaaaca agataaatcc cagctgatcc
      361 atcgtttgat cgatctttta cggtttacga cagtcaaata cactcgatca aaagttcgaa
      421 aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc gatgatcgta
      481 tcaacgggaa aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg atgaaggaag
      541 aagaaaaatt tttaacaaaa cttgcgcaga cgatccaaaa atctgtcatc gatcatttgc
      601 atatcgtggg tgatattttt gatcgaggaa ctcaagcagc caaagtaatg gatcgaatca
      661 tggatttttc ctctgtagat atccagtggg ggaatcatga tattttgtgg attggtgcgt
      721 attgcggctc tgaggcatgt ttattgacgc ttttacggat tgccgcacgc tacaactatt
      781 tatttgaatt agagaaagaa tatggattaa atttacgtcc attattttct tttgcccatc
      841 aaacctatca gaagaatcca gtatttaccc ctaaaaacag gaaagatcta tctgaaagag
      901 agcaagaaga attagaaaag atccatcaag cactttctat cctccaattc aaatcagaac
      961 atcagttgtt gaatcgacga cctgaattta aaatggacga tcgtaaattg ttggaaaaaa
     1021 tcaactatga ggaatcaacg atcgttttag aaggtcagct ctatgagttg aaagggactt
     1081 gttttcaaac gatccagcca gatgatccta aaaaattctt ggaagaggaa caaaaagtga
     1141 tcgatagttt gatgtattca ttccagcact cgttaagaat gcaaaaacac atgacgtttt
     1201 tgatcgaaaa agggggaatg tatctcacga acaatcagca tgtactgttc catggctgta
     1261 tacctgtaga tgaaaagggg cagctcttag catttgaatt agaccaatct tatcgcggaa
     1321 aagaactgct gggatttttt gaaaaacaga taactgaaag cgcaaaagat ttgactgcaa
     1381 atgaagattt ggcgaccgat ttgatttggt atgcgtggag cgggccattt tcgcctttat
     1441 tcggcaagag taaaatggca acttttgagc gctattttct cacagagcct catacgcacg
     1501 tcgaaaaaag caatccctat tatcacttgc gagatgaaga gtggttcagt gaaaaaattt
     1561 tggcagaatt cgatgcaaaa gaagaaggtt cagcaatcat caatggacac acgccagtaa
     1621 aagtaaaaaa aggagagtca ccgatcaaag caaacggaaa agcatttgtt attgacggag
     1681 gtctatccaa agcttatcaa aaaacaaccg ggattgctgg ctactcatta ctatgcaatt
     1741 cttacggttt ccaaatcgtg acacattatc cgttcttgcc tattgaacaa caatttgcaa
     1801 aaaaatctga tcaaacgaat gtaaaaaaag tgatcgagca gcagctccca cgcaaactta
     1861 atcggaatac gacaaaagga atggaattac agcaacagat tactcaactc caaaggctgt
     1921 tggattatcg aaaggacgat tgagaaaaat gctagccaac attgctcaat agactaaaaa
     1981 gcgactgcaa caaaaggcgt gtcgcagtct ccttcggaat tagctcaaaa aacggtaaaa
     2041 tattagaagc tatttttctg tttatttctc taatactcag gtcagggcgc tgatttcacg
     2101 acctcttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccaac
     2161 aaacatgaga agctgttttt tgaaattgct ccttgtgact cagagttgaa cggcaggctc
     2221 acgacctaat cagaaaaacg attaaaattc attcacaaag aaatcgaata aacgctgttc
     2281 agatgaatct acacgatgag aaagttccgg atgccattgg acaccaagga ttttgtgcat
     2341 attatcttta ctttggactg cttcaatgag tccatcgttg gaaaaggcga tggcttttaa
     2401 caaaggagaa agatctttga ctgcctgatg atgataggaa ttcacaaagt atgaatcagg
     2461 gagaaggtca cttaataaac tgtccgaaac gattttgact tcgtgagttg caaactgcgg
     2521 ggcagtgggc tgttgttcgt gttttacgga ccattttgga tataatgaaa gatcttgata
     2581 aagtgttccc tccaacacga cattcaatag ttgaagtcct cggcagactg caaaaatcgg
     2641 ttttctttgt ttcaatgctt ccaagatcaa tgcctgttcg aattggtctc gttgaatatt
     2701 tgtttccgtc agttttggat gagggtcttc catgtaaagt tgcggactaa catcctgacc
     2761 gcctgcaaga atgagtttgt cgattttctc aattagttga ggcgctgaat ctggagacat
     2821 gactggcaga ataagaggta atccttgagc atctaaaact gcatccacaa atccctgcgg
     2881 tgtatagctt acttgatttc cttggaaagt atctgtcgct cgaatcagtt gattcccggc
     2941 aatgccgatg attggtttca tttttattcc ccctaaagta gactaattgt aacaaggatt
     3001 taaaaaaatt taaactatgt aagacttgcc cctatactta tctgttcttg ctgtgttact
     3061 atctgtaaag agaattttcg tgtgaaattc atgacgatag ttgaaaatat tgcttaattt
     3121 gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag gatctagttc
     3181 ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt tatctttaga
     3241 ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg aaaggaagtg
     3301 aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa tgagaagcgt
     3361 gctcgaaccc tacggcagca gttgaagacc tatgcagaag aaaaagagca agaattggag
     3421 ataatccaaa cgaacagtcg gcaacagctg aaacagttgg tagaagaaaa agaacaattc
     3481 gaaaagcaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga caaggtgcaa
     3541 gcaactttga aagaccaata tcaaaagcat cagagtcaag caatcggtgc aatcattgaa
     3601 agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca tttcagataa
     3661 aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaatgttg aaatccggga
     3721 tctgtatcaa aatagaacaa acaaccagtg ggtagaaaaa tattttccag ataccacgat
     3781 tattgataaa gaagcaaaat taagcgcttt aggcaatcgg ctgaaagaca tccaagaagc
     3841 tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt tgaaacgtca
     3901 agaactttcc ttacaagact tggaagcaaa ttattccgaa gaaaatttca tgaataaatt
     3961 agaagaagtc cttgatttaa aagaaaagtg ggaacagcta actgaaaaac gtgatcgttt
     4021 agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc caagtatgta
     4081 tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt ttgaaagttt
     4141 caaggaagaa ctaagtaagc ttgatgacgt ttatatggaa gaaatagatt atacgccaaa
     4201 acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgctatag aagaaattgc
     4261 tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta aagaaaaatt
     4321 gcaagaaacc aaacagcaat tatccattgt tcacgagcag caaaaaaaga taacgacagc
     4381 gttaggtcag tgcagcggct atatccaaga atttgaatgg gtagaagaag tcacacttgc
     4441 cgttgcagaa cgggaaaaag taaaagaacg gtttatccat gccacttatc tgatcgtttt
     4501 gcaaggttgg gtagatgcag aagaaaaaca gaatttgatg catgtgttaa acgaaactgt
     4561 atcagaagaa gatatatacg ttagtttcga aacaccaaac tcagaagaga ttcagcagga
     4621 agtccctact aaattgaaaa accatccgct tatcgaacct tttgaattgc tgactgaaat
     4681 gtacagtctg ccaaaatatg aagaagtaga tccaacgcct tggatgacac cattttatct
     4741 tgtcttcttc gggatgatgg tcgcagatgt tggttacgga ttgctgatgc tcattgggac
     4801 aatactagct caaaaactat ttgttttacc ccgcgggatg aaacgtttcg taaaattctt
     4861 tgagattctc tcggtaccat cgatcatctg gggattgatc tacagttcat ttttcggaca
     4921 ggcattacca aaagaactat ttggtatccg cttgcctttc ccaatcctat ctacgacgga
     4981 tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga tcttggttgg
     5041 attattcgtg gcagcaaagg aaaatatcag gaggaaagat tacttgagtg ctgtcagtga
     5101 tggttttgcc tggcaaggga tcctgattgg gatcgttatt gcattgattg gtgtcatgtt
     5161 gataaaaaat acagcatttg tttatcttgg cgcggggcta tcgatcattg ctgctttatg
     5221 tatcttaatc gtgccgatta tccaatcgcc gtctaaagtc aagggtgctg ccaaaggagc
     5281 gtataatctt tacggattga ctggttatat cggtgattta gtcagttata cacgtttgat
     5341 ggcactaggt atttcaggcg gaagtattgg cgcggcattc aatatgctag ttgctttcat
     5401 gccgccagcc gctcgattca gtgtagggat ccttttgatc atcgctttac acgcactgaa
     5461 tatgtttttg acactgttaa gtgcctatgt ccatggtgca cgattgcagt atgtcgaatt
     5521 ttttggaaaa ttttatacag gaggcggacg tgcctttgac ccgctgaaaa cggcggaaaa
     5581 atacgtcaac atcaatcata agaaaaaaaa caataaataa ttggaggatt tttatacaat
     5641 gatggattac ttaattagtc aaaatggtgg aatggtattt gcagtattag cgatggcaac
     5701 agcgacgatt ttttcaggga ttggttcagc taaaggtgtt ggaatgacag gtgaggcagc
     5761 agcagcgtta acaacaagtc aacccgaaaa attcggacag gctttgattc tgcaattgct
     5821 tccaggtacg caagggttgt atggattcgt tatcgccttt ttgattttta tcaacttagg
     5881 aaatgatatg tcagttgtac aaggattgaa ctttctaggc gcttctctgc cgattgcttt
     5941 tacaggtttg ttttcaggta tcgcccaagg gaaagttgca gcagcaggta tccaaatttt
     6001 agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg ttgaaacata
     6061 tgcgatttta ggtttcgtta tttccttcct attagtattg aatgcctaag cataaaggag
     6121 gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaatgaagc agcagagcaa
     6181 gaaagagcgg cgttagagca agaagagcga atgaagatcg atcaaaactt tgaacaaaaa
     6241 cgtacgcaaa tcgaaacaga acatcaaaaa caaaaagaaa aacaaatcga attacttgaa
     6301 aaaaaatacc gtcagcttag gaatcgccaa caagtagaag tacgacaaga aaatttgaat
     6361 gcgaagcagg aatttttacg acgattgttt gcagatgcaa tagctgaaat ggagaattgg
     6421 gatgaatctg aacaaatcca gtttatcaaa aattcattgt attcattacc tttgacaggc
     6481 aaagtaacat tcattgctgg agaaaaatca gcagcttatc tttctcagac attgctggat
     6541 gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt tgctgatcaa
     6601 gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag cagccttgta
     6661 caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga ataaggaggg
     6721 atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag cttgaacttt
     6781 tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca cttggtgaaa
     6841 tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat gacttcgaac
     6901 agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca gcaccggagc
     6961 ctgaaatcgt ttggatctat acgatgcgct atactttcca taatctgaag gttttgacaa
     7021 aagccgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga ttttattcat
     7081 tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaactccct gaaagcatta
     7141 tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt cagggaattg
     7201 acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat aagctaggat
     7261 atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc acaacgatgg
     7321 ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt tccagctcgg
     7381 gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa gattacacga
     7441 attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt gagggaaata
     7501 ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg tatcaagaag
     7561 cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca aaagaggtag
     7621 aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa tcagaaacaa
     7681 tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg cgacaaagat
     7741 tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac caagcaaacc
     7801 gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta tgtcacagag
     7861 caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat gatacctgct
     7921 atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga aattcaaaaa
     7981 aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat agaaattttg
     8041 caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa catgtctgat
     8101 gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga aattatcgag
     8161 atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat tggtccaggc
     8221 gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg gatcatttca
     8281 caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac acaaagtaat
     8341 ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg gtggttcgaa
     8401 ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat cgtagaagaa
     8461 acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac gatcaaaaca
     8521 atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac cgaaaatggt
     8581 gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc aatcaaagaa
     8641 aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac attcttccca
     8701 gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa aacagtcgtt
     8761 cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg atgtggcgag
     8821 cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga ccctaataca
     8881 ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat gccagtagca
     8941 gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg tgacatgggt
     9001 tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact tcgtgagatg
     9061 agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct tggttcacgg
     9121 cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga aggtcgcgaa
     9181 ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc ggaaccagtc
     9241 acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag tttagcccaa
     9301 aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc aacagaagta
     9361 gggaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac cgagggcatg
     9421 cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg gattgattcc
     9481 ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga agattatttg
     9541 cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca attcaaaatg
     9601 ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt aggtgcttat
     9661 ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg aagcaaatat
     9721 atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa gcaaacgatg
     9781 caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta cgatcggtga
     9841 agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg aagaattaat
     9901 cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag tccaagaaga
     9961 taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg actcttctgt
    10021 acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg gtcgagtatt
    10081 cgatggtttg ggacgaccga aagataacgg accagagatc ctgcctgaaa agcatttgga
    10141 tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt ttatccaaac
    10201 aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat taccagtatt
    10261 ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac aagcaacggt
    10321 actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca cttttgaaga
    10381 agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt ctgtcatgtt
    10441 tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta tggctttgac
    10501 tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca tgacagacat
    10561 gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag ttccaggaag
    10621 aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac gagcaggaag
    10681 aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc cagaagacga
    10741 taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga tcatcttgac
    10801 tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt ctctttcgcg
    10861 gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag ctacgatgaa
    10921 tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg tagttttagg
    10981 tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc ggtttgaaaa
    11041 agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac ttgatctagg
    11101 gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag acgacatgct
    11161 ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg tgaatccgac
    11221 ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag ggcataaatt
    11281 attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc gaaagaacaa
    11341 ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt ttgtattggc
    11401 aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg cggaaaacgt
    11461 tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga tgaattttga
    11521 atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt ccagtgccga
    11581 actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg aactgacaga
    11641 aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc ggcgagtcaa
    11701 tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata ttcgtatgaa
    11761 gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa atatgggtac
    11821 cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca aagcaaacaa
    11881 ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct cctgatttta
    11941 gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg gacaaatttt
    12001 atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac aacattgaat
    12061 acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat tgagatcggc
    12121 ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa gaaagtcagt
    12181 ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc aggtgtgatg
    12241 aagctcatgc tttacatcct aaaattggcc tttgtgatcc aattgattgg tacggttgct
    12301 ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta cagtttgttc
    12361 catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag tttagccaat
    12421 caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc tggtgggtta
    12481 ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat gacgattcat
    12541 tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt tgtgttcttt
    12601 ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg attggcaaac
    12661 acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga ttacctccat
    12721 atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg aacatctggg
    12781 tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca gatgcgttcg
    12841 atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca aggcgcagtg
    12901 ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc catcatgatt
    12961 ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc ttttgaggtc
    13021 ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt gactgcaata
    13081 gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt gactgtggtc
    13141 ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc agaagaaagt
    13201 gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa aataagaaaa
    13261 taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt tattttttga
    13321 ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg aatcaataag
    13381 atttgattcc tttatttatc aataggttat aattcatatt tttttatttt ttcttttacg
    13441 atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat tcgaacgaat
    13501 gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat accgttagca
    13561 agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag aattcgatag
    13621 taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag ctgattggct
    13681 ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga atggaggatt
    13741 tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa aattgcttgt
    13801 tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa tccagaacaa
    13861 ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt ttggaaagga
    13921 ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc ttcaataacc
    13981 catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg agagatttga
    14041 aaccctatag gattattaca agaaggcata agaaagacac catgaatctt cttttttcta
    14101 cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat tggaacgatc
    14161 tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg gtctgttgct
    14221 attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt ttgaacaccg
    14281 gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa ccaatttgct
    14341 gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa accaacaaga
    14401 tttgggttat tactagttga ttgaataaac gggacaacga atttattaca ttcttcaaaa
    14461 gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga gatggtgatg
    14521 gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata cagcaaacct
    14581 ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc taaataatca
    14641 gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt taaaatatct
    14701 tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg tttgtctggt
    14761 cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac tccttaaaat
    14821 tgtataccat acaattttac cattgattgt attgatttta gaagttaatc ttttgtgagg
    14881 agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt tagttttgaa
    14941 gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa aatcagccaa
    15001 aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt tgcgatgatg
    15061 cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc ttttccggat
    15121 aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga tgctcaaaac
    15181 ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac agctgctgta
    15241 tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc tttaattgga
    15301 gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc catcactaca
    15361 gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa agctaaaaaa
    15421 cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt acgtccagcg
    15481 gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga gtgggtaagg
    15541 gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga gataagtaca
    15601 tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt aacagaaagc
    15661 ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat tgttggtaca
    15721 ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag gccgacgatt
    15781 ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat ttataacaaa
    15841 ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca tttgcagatg
    15901 aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg ccagaaggta
    15961 cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca gtgaagaaaa
    16021 accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt tgtggtcacg
    16081 cgacattagc aacagcattt gttttattta actattacaa aatacctgat gaaacgatta
    16141 aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat tattacatgg
    16201 attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa gaagcgattg
    16261 gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta gaagatgaaa
    16321 aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa ttaggaatag
    16381 gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc ttctttccaa
    16441 aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt attccttatt
    16501 gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca aggggcgggg
    16561 atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca gttttgtttt
    16621 ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat tggtggagag
    16681 tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc aatttaatga
    16741 aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa gagtaaaatg
    16801 ccccatacta actatcctcg gatgacttcg attttataac catcagggtc aatgacaaag
    16861 tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag accagcagct
    16921 ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg accatagccg
    16981 tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc atagtcatcc
    17041 ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg gcggcggctt
    17101 tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc tttcactcga
    17161 acacatgtgt gagccatttt catagattca tctctccttt tccttttact caatcataac
    17221 aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct catgtaaaat
    17281 ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat ttttggtgaa
    17341 aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat agaaagagat
    17401 cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg cggggaaatt
    17461 gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga aatgggaatt
    17521 gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc gaattatcgt
    17581 gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa gcgtatagga
    17641 gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc gcttgtgaaa
    17701 ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa taaaaactga
    17761 aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta catatttttt
    17821 gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg ctgcttcttt
    17881 gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa tcatggataa
    17941 gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa ggtgctgtgc
    18001 gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga agtaataggg
    18061 cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg caattgctgc
    18121 atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt ctggtgtcat
    18181 ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt ggatcgtata
    18241 agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat ggagatgact
    18301 gccgacacgg cgtgccatta cttcgatctc taggaaatct tcatgtgtca gtccgtgacg
    18361 ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag taataacgta
    18421 atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg gtaagggacg
    18481 gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg cttcttttac
    18541 ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga tagcgactag
    18601 tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg cgccattacg
    18661 gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat cacgcaagga
    18721 cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt gtcccgctaa
    18781 tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat ttcgcaaaat
    18841 ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag gtagatcgta
    18901 gctagaattc acagcagtgt aataaacagg attatatccg accttttcta ctaacaaagt
    18961 gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt tcagtggttt
    19021 acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt cagaccattg
    19081 agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta aagtaaagct
    19141 tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg catcttcttg
    19201 atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac cacgggaaac
    19261 aggatggaag tacacttgca catacatttg atatcggcta acgatataat cttccactgc
    19321 atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca cacgtaatat
    19381 tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc gaagcaaata
    19441 gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg ggttggggta
    19501 agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc ggttaagaat
    19561 ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt catgattcgt
    19621 atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat gcaacagtgc
    19681 cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta ttttttcttt
    19741 tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa cgcctaatga
    19801 atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc ctaattgttt
    19861 aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta cttgatgctg
    19921 gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca gcatttgata
    19981 tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc attctctgga
    20041 tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta gaatcctgta
    20101 aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg acttctttta
    20161 caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga tccactgcag
    20221 gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc ttcatgactt
    20281 gcccacgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa atcccatttt
    20341 tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa tcataagttc
    20401 caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga aaagcgtatt
    20461 tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt gtgtcacttt
    20521 gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct aatccacctg
    20581 aatttctgat cactggagcg taacctgcat cttccagtgt ttggatcccg tcacttagaa
    20641 aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc cagaaatgta
    20701 aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat gcaaaagggg
    20761 aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc attcaaccgg
    20821 ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa gagttgtttt
    20881 ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag aaactaggaa
    20941 caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag gaacaccgat
    21001 ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt tttattttga
    21061 tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata aagagattca
    21121 ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg gtcatgtaca
    21181 gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag aacggttaga
    21241 aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga aagaactgca
    21301 tgttagtttg aaagaccgtc ccacagcagg gaaggttcga atcgattatg atctgttcat
    21361 gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt aaatataaaa
    21421 gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt gaaaggacgt
    21481 gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat caatgatcga
    21541 agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg atttagctaa
    21601 ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac tcgcacaatt
    21661 ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc gttggggctt
    21721 gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa tggacgaaga
    21781 ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta tcaatgatga
    21841 tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga cagaagaaga
    21901 tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg cttataattc
    21961 tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact tgccaggaaa
    22021 tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt atgaagagga
    22081 agaattttct gactctgaaa aagaataaac attattttct tacgagaggt tgtgacagag
    22141 acgatcagcc tatcagaata aggaggtgtg acacgatttt tgtcacacct tcttttttgt
    22201 atataaaaat cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt agcgcacctt
    22261 gtacgtcata ttaagtgagg cagaaaggag tggagaatga tttttactgg atataagaaa
    22321 aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa
    22381 aaaatgttcc gtatcacgat gaattacgta catgataaag aagaggcctt agatgtgatg
    22441 caagatagtt ttcataaagc cctgaactat tttgaaaaag aaaaagagat cgagcatttt
    22501 agtgcatggt tttatcggat attgatccgc acggcattag atagttggcg cagacaaaaa
    22561 aggacgcctg tggacttatt tgaaattcaa gatatccccc aacagttacc tttagaaact
    22621 tccgttgcag agctccatat gattttagat aaaatagaca gtccggaaaa agagattctg
    22681 atcctctact tttttgaagg atttcgtttg aaagaaattg cgactatctt ggatatgaat
    22741 gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca aatcctagaa
    22801 tgaaggagag aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac
    22861 ttataaagaa caagcaattc ccaaagggct taaagaggag atccgccagc gttttattat
    22921 cgaagagcaa tcatttataa aagcaaagcg gagaaagaga cgtctccaag ggttcggagt
    22981 atctttggct atcgtaatga ttacaacaag tagccttctt tttaatagtc aagttcgaag
    23041 ctttgcagaa gatttaccca tcttaggatc agtcattgaa ttgatcttag gcgaacgctt
    23101 tactgatcga tctgaaaaaa tcgatattca ggtaccgaag atcaatacac aaaatgagaa
    23161 agaaaataaa acaattcatg gattaaatca aaaatacttt cgcgaaggac aagcagattt
    23221 tgaaaaagct gaaaaagaat atggaaattt tgaaacggat cattaccaag ttttgggaga
    23281 ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac
    23341 agcagcagat agccatgttg aaaaaagata cgacacaatc gacaagaaaa acagtgtcca
    23401 gctctcactt ccacttttat tcaaagatga cacctatcta tctgttttga caaaagaagt
    23461 caaacggcag atggccgaac aagtcaagga agatccatct aagtactatt ggactgagca
    23521 agatattcaa gaagggacga tagaaaaacc aacacttgtt acgcctacac gaagttttta
    23581 tttgaacaaa gagcatcaat tagttttgac tttttctcaa tacgaaattg ctcctggtta
    23641 tatgggtaca ccagaatttg tcattcctaa gtccgtgaca aaaacgattt tggcatcgga
    23701 agattatttg gatcattaga cgagaagtag ttgcgactag cagtttcaat tttcaagtaa
    23761 gtacaagtaa ataataagaa gagaaaaaga gaaagaagag atatagtgaa aagaaattat
    23821 ttagttatca gtattattgc tttatccgtg cttttgtttt tcgaaattac ttcggtggcc
    23881 tccttgatca ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc acaatctttc
    23941 aaacaatcag aacaatcaac cattaaaagc caatcaactg aaacagctaa cgtcaaagaa
    24001 gccattcaag aatcagcgga aaaggtacaa agtcagtcat cacaatcaac acaagacagt
    24061 acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca
    24121 ggagcgacca caacaccgca gcttctgcga atcttaaaag aaaaaaacgt gcatgccact
    24181 tttttcgtct taggagaaaa tacggcgcag catcctgaaa tcgtgaagca gacagctgaa
    24241 gcagggcacg agattggcaa tcacacatac gatcatcaga atctggccac tctttctgcc
    24301 cagtcgatga cggaggaagt gacgaaagca gacactgaga tcaaaaaagt cactggcaaa
    24361 acccccactt ttgttcgtcc gccctatggt tctgtcacga atgtaggagc aacgatcatc
    24421 cagcgaccga tcatcgaatg gtctgttgat tcagaagatt ggaagacaag aaatcctgat
    24481 ttgattttgc agaagatcca agcaacagta tacgatgggg caattatttt attccatgat
    24541 atctatcctg aaacgattcg tgccgttcct caagtgatcg attatctgaa agagcaaggg
    24601 tatcgcatca cgacagttgg ggatttatta gggcatccaa cggctgtcga gaactattat
    24661 ggcagaaacg atcatcggcc agttcagtaa gtgagacaag tatctttaat aaataacaca
    24721 ttttaataag tagttgaaca cataactacc ttacttgcaa aggcttaaca atatctatac
    24781 aggtacaaca aaaaactgtc caccaattt
//
LOCUS       DAXWLH010000024        24637 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXWLH010000024 DAXWLH010000000
VERSION     DAXWLH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.024"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            85..1785
                     /locus_tag="ACGE36_000712"
     CDS             85..1785
                     /locus_tag="ACGE36_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFF8383131.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1755..1973
                     /locus_tag="ACGE36_000713"
     CDS             1755..1973
                     /locus_tag="ACGE36_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFF8383132.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1993..2883
                     /locus_tag="ACGE36_000714"
     CDS             1993..2883
                     /locus_tag="ACGE36_000714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFF8383133.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2901..3185
                     /locus_tag="ACGE36_000715"
     CDS             2901..3185
                     /locus_tag="ACGE36_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383134.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3672..4286
                     /gene="gmk"
                     /locus_tag="ACGE36_000716"
     CDS             3672..4286
                     /gene="gmk"
                     /locus_tag="ACGE36_000716"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8383135.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4291..4596
                     /gene="rpoZ"
                     /locus_tag="ACGE36_000717"
     CDS             4291..4596
                     /gene="rpoZ"
                     /locus_tag="ACGE36_000717"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8383136.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4849..7257
                     /gene="priA"
                     /locus_tag="ACGE36_000718"
     CDS             4849..7257
                     /gene="priA"
                     /locus_tag="ACGE36_000718"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFF8383137.1"
                     /translation="MILTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7272..7763
                     /gene="def"
                     /locus_tag="ACGE36_000719"
     CDS             7272..7763
                     /gene="def"
                     /locus_tag="ACGE36_000719"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8383138.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7756..8694
                     /gene="fmt"
                     /locus_tag="ACGE36_000720"
     CDS             7756..8694
                     /gene="fmt"
                     /locus_tag="ACGE36_000720"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFF8383139.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8694..10052
                     /gene="rsmB"
                     /locus_tag="ACGE36_000721"
     CDS             8694..10052
                     /gene="rsmB"
                     /locus_tag="ACGE36_000721"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFF8383140.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10065..10805
                     /locus_tag="ACGE36_000722"
     CDS             10065..10805
                     /locus_tag="ACGE36_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFF8383141.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10802..12871
                     /gene="pknB"
                     /locus_tag="ACGE36_000723"
     CDS             10802..12871
                     /gene="pknB"
                     /locus_tag="ACGE36_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFF8383142.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13036..13935
                     /gene="rsgA"
                     /locus_tag="ACGE36_000724"
     CDS             13036..13935
                     /gene="rsgA"
                     /locus_tag="ACGE36_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFF8383143.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13948..14598
                     /gene="rpe"
                     /locus_tag="ACGE36_000725"
     CDS             13948..14598
                     /gene="rpe"
                     /locus_tag="ACGE36_000725"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8383144.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14599..15237
                     /locus_tag="ACGE36_000726"
     CDS             14599..15237
                     /locus_tag="ACGE36_000726"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFF8383145.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15392..16246
                     /gene="mreC"
                     /locus_tag="ACGE36_000727"
     CDS             15392..16246
                     /gene="mreC"
                     /locus_tag="ACGE36_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFF8383146.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16250..16759
                     /gene="mreD"
                     /locus_tag="ACGE36_000728"
     CDS             16250..16759
                     /gene="mreD"
                     /locus_tag="ACGE36_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFF8383147.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17014..18588
                     /locus_tag="ACGE36_000729"
     CDS             17014..18588
                     /locus_tag="ACGE36_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFF8383148.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLHQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(18656..20455)
                     /locus_tag="ACGE36_000730"
     CDS             complement(18656..20455)
                     /locus_tag="ACGE36_000730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFF8383149.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            20578..21084
                     /locus_tag="ACGE36_000731"
     CDS             20578..21084
                     /locus_tag="ACGE36_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8383150.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21163..21885
                     /locus_tag="ACGE36_000732"
     CDS             21163..21885
                     /locus_tag="ACGE36_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8383151.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(21963..23363)
                     /locus_tag="ACGE36_000733"
     CDS             complement(21963..23363)
                     /locus_tag="ACGE36_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFF8383152.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(23533..24507)
                     /locus_tag="ACGE36_000734"
     CDS             complement(23533..24507)
                     /locus_tag="ACGE36_000734"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8383153.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
BASE COUNT         8019 a         4552 c         5256 g         6810 t
ORIGIN      
        1 ttcttatgga atctacaaag tcgaatacga gactcatgac ctttttatga cgtactggta
       61 tacttgaaga aaaaggagag cgcgatggag ataaaaaaag tttacttttc acagaaaatg
      121 tcgctgtatt cgcctatcca gcaagcaaaa caaccccttc cattttttca aagtcatcaa
      181 gaaagtagag acgctttttg gcgttctgca aaaaagcaat gtgcttttaa agcagtatcg
      241 aaaaaatata ccgtctattt tactattgct aaaagtagag gagatgttat ttttaataat
      301 ctgttgattg aaggtgaaac ttttgaatgg aaaaaatttt ttcggtatat ggaagcttgc
      361 gctcgttttt ttattaaaga aaactgttgt tttttatttc aatctcctct atcagaagaa
      421 tggttgcgta tttttcataa aaacggctat caaggaacca cacaattaaa taaaaagctg
      481 gtctatcata cagcacttgt tttaggtgga ggaggagcac acggtgctta tcagattggt
      541 gtgtggcagg cgctgaagga acatgatatc aactttgaga ttatcacagg tacctcggta
      601 ggtgcgttga acggcgcatt gattttacaa ggagatatga aaaaagctgt gaatttatgg
      661 aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa tggctgcagt tgaagacctt
      721 cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa cagcactaat tgaaggcggg
      781 gtttcttctg ctccattgga aaagatggtc aaagacaatt tggatttgtc cttactcaaa
      841 cataatccca aaatacgttt atatacggtg tctactaaac tgccagaact agcggaagta
      901 gtgactgata tccaacagac aaaaacagaa gaaataccag attggatact cgcctctgct
      961 tcattttatc ccgctatggc ttatcgaaag atcggaaaaa ataaatatgc agacggtggg
     1021 tatcgcaaca aaatacctat tgatattgcg attaacaaag gagcaacaga ggcatttgtc
     1081 gtagatgtgc aagggccagg cccagcgaaa aaaattcgta tgccagacat ttttatccat
     1141 tggaaatgtc aaacattgtg gacactaggc agctttctgc tttttgatag ccaaaggaac
     1201 caattaaatt tacaattagg ctatctggaa atgaaaaaac gattaggcta ttactatggg
     1261 aactggtata catttcattc tgttaaacct gcaagaacat actggcgagg atttttgtct
     1321 tacttaacaa aagaaattca gttagaatta tcttttttca aaagcataaa attttggcaa
     1381 aaactgcgta atttatataa gaacagggtc gtaccagaga catgcggatt ggcgatgcta
     1441 gaacttttag caaaaaaaca ttttttactg cccaatgaaa tctatcaagt agacatgatg
     1501 attcaaatga tctgccaaca agatataaaa agtatgccgg atgatttatt ggcacaaatt
     1561 ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat accagcaaaa aatacaaaat
     1621 gagcgcacga aaactgttta ttttgattat ctgctacaag gaaaacgcaa ggatcgtttg
     1681 cagcatgaat tgcttaagca accagttgac acattactga tcctatattt atattatctc
     1741 aaggaggaac aaccatggca caagaatttt catatgaaat cgtagaagaa atcgctatat
     1801 tgtcagaaaa caacaagggg tggcgtaaag aactgaacct agtcagctgg aatggtcgcc
     1861 cgccaaaatt cgatcttcga gattgggcgc cagaccatga aaaaatgggg aaaggcctga
     1921 cattgacgaa cgaagagttt gaacaactgc aaaaagcaat agaaaatatg taatgaggaa
     1981 ggaccctgtc gaatgaaaag tatgactggt tttggcaaag ccatccgcga aacaaaagcg
     2041 tatcagttag atattgaaat aaaaagtgtc aaccagcgtt ttctagatgt tcaaatacga
     2101 agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc agcttattaa acagcagtta
     2161 agccgcggtc gaatcgaagt atttgtgaat ctagcttata ttggcaaaaa cgaaaaacag
     2221 atcgtcatca actgggaatt gattgattca cttatcactc aattgacaga cggaattcaa
     2281 caacgatacg gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg cttcgcgctg
     2341 gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga attggcacag
     2401 ttagctctag atacattgaa aagcgcactt gattctattg aggcgagtcg agaaaaagaa
     2461 ggtcaagctt tagctttagt gttgaaaaag aatactgaag aattcaaaga agtactaaaa
     2521 aaactcgata gttttgtaga aatctatgaa aaggaatttc aaataaggta tcaaaaaaag
     2581 ctggaggaat ttcttggagc gaaagtcgat gagcaacggt tgttgacgga gctagccatc
     2641 ttgttggaac gcggagatat tcacgaggaa ctggatcgtc tggcgatcca tgtacaaaaa
     2701 ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg aattagattt cctgatccaa
     2761 gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga aattaaaaat
     2821 caagtcgtcc agtctaagac gatcttagaa aaaatcagag aacaagtaca aaatattgaa
     2881 tgacgaaaga ggtagagata atgaaatatt atgacgtaac gttccacgaa ttaagtggaa
     2941 aaactgtggt taaacgggat atcccttctg aaaaaaacgg ctttgacgta tggaaagatg
     3001 cctgtgcaga ctataatgag aacgaactgt ttattttgat caacgacggt gcttacgtga
     3061 cgatgaatcg gaaatttatt gttcgaattg acacagaaga ggtagaagat ccgacagaaa
     3121 aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc aatatgggct
     3181 tttagcaagg atgctgaaca aagaaataca ataaataatc agaattggat ttcttcgctt
     3241 agcttctttg ctctaggaat acatttaaag gttgtgacaa aattcgtgtc acaatctttt
     3301 tttattttga ataagtgatg ttcaaaaacc attttctcgg tattaagcca aatttatgca
     3361 aaaatgtgag aaactatttt cacaaaattt ttcttcatac tcgaaactaa tcggcttctt
     3421 cacgacctct tatacacggc gttcaaaagc ttgcatctgc gataataacc ccaaccaaac
     3481 gaaaaacatg agaagctgtt tatgtttggc tgttcttatt acttgatgca ggacagcttt
     3541 tttacgacct cttggtacag ggtagctttt ttaccaattt ctagctataa ttaaagataa
     3601 ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa gtacaaaaag actagttgaa
     3661 aggacgtcgt catgtcagag cgtggattat taatcgtgtt gtctggtcca tccggtgtag
     3721 gaaaaggcac agtacgaaaa gcaatttttg agagtgagga taatgatttt cagtattcga
     3781 tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga aggcgttgat tatttcttcc
     3841 gaagcaaaga agagtttgaa tcaatgatcg aagctggaga gatgctggaa tacgcagaat
     3901 atgtcgggaa ttattatgga actcctttgt catatgtcaa caagacattg gatgaaggaa
     3961 aagatgtttt cttggagatt gaagtccaag gagcaaaaca agtcaaagaa aaagtgcccg
     4021 atggtgtatt catctttttg actccgccag atttagcgga attaaaatca cggatcgtag
     4081 gccgaggaac agatgcagat gaagtaatcg aagaacgaat gaaagttgca aaagaagaaa
     4141 tcgaaatgat ggctctttac gactatgctg ttgtaaatga tgaagtgccg ctagcagttg
     4201 aacgaatcaa aaacattatt gccagtgaac attttcgagt agatcgcgta atcggtaaat
     4261 atattaaaat gttaaaggag atgtagtctt atgatgttaa aaccttcaat tgactcacta
     4321 ttagatcgtg taaattcaaa atattctcta gtgattttag caagtaaacg cgcgcatgaa
     4381 ttggatgcaa gtgcacagcc tactctagat tcttttgatt cagtaaaaag cgtcggtcaa
     4441 gctttagaag aaatcgatgc aggaaatgta gtcaatgatc cacatcctga attaaaacgt
     4501 gaacgtttga agatggaaga agaggaacgt caagcacaaa aagagcgtga acaacacgaa
     4561 ttggaatcac gtatccgtga agagcaaaaa atgtaataaa gaaaaataga gcgaacatct
     4621 aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt cgccacttat tgtctggtag
     4681 aaaaggctgt gtctgaactt tttgacacag ttcttttttt tcttatctgc tgttaatttt
     4741 ttgttaggat ttggcagaag ataagacaaa tccatcgatc tctagtaaaa taggcaagga
     4801 actttttgtc gcttgataag aaaagacaaa aaaaggagga aacgaaccat gatattaaca
     4861 gctgaagtca ttgtggacgt acctactatg cagaccgatc agccattcac ttatttggtt
     4921 ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag tagaagtccc ttttggtaat
     4981 ggcaatcgtc atgtccaagg atttgttatg gctatccaaa aatcagaaga atcagctaat
     5041 ccatctttga aggcagttat tcgtttgcta gaccttgctc cggtggtcaa tgaagaatta
     5101 ctttcattag ctgattatat gaaaaaaatt acttacgcat tcaaaatcac ttgtctacaa
     5161 acgatgcttc cgagtgtgat gaaagcagaa tatgataaat tgatctatcc tttggcagac
     5221 acaccagaag ttcaggtatt gtttgatcag cgagaagtga tttcctggaa ggaagcagaa
     5281 gaagaaggca gcctttctca attgattcgc tggcgccaag agcaattagt agatatcaaa
     5341 tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt tggtccgttc tttgttaact
     5401 gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa atgccaagaa acaaaaagaa
     5461 cttcttttgt gtttatctga actgagccaa gaagaaccaa ttgcttattt caaaaataaa
     5521 ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg gctggctgga gtttatagaa
     5581 tcagaacgtt atcgcgaccc ttataaagac cgagtatttg accaaacgac ggcattggaa
     5641 ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca cagcaggtca gcagcaaaaa
     5701 gatcaggtct ttttgctgga gggaatcact ggcagcggga aaacagaagt atatcttcag
     5761 gcaatcgctg atgttttgtc agaaaataaa acggctatta tgttagtgcc tgaaatcgca
     5821 ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg gagaatcagt tgcggtctta
     5881 catagcggac tttcccaagg tgaaaaatat gatgaatggc gcaagattga gcgggaagaa
     5941 gcgcaagtag tagtaggcgc acgatctgct atttttgccc cgctgaaaaa tatcgggctg
     6001 atcattatcg atgaagaaca tgaatcaagt tacaaacaag atgagacacc gcgttatcat
     6061 gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt gcccaatagt tctaggaagc
     6121 gcgacgccct ctttagaatc aagagcacga gctcaaaaag gcgtttatca gttattgcag
     6181 ttaaaccacc gagccaaagc agctgcacag cttccagcta ttgagatcgt agatatgcgt
     6241 gaagagtttc agaatcatcg gacctctact ttttctgcta atttgcagga aaaaatacaa
     6301 aatcgattag acaaaaaaga acaaactgtc ctactgctca atcgtcgagg atattcgtct
     6361 tttgtgatgt gtagggactg tggatttgtt cttccatgtc ctaattgcga tatctctctt
     6421 acactgcata tggatacacg ttcaatgcgc tgccattatt gcggacatga agaacctatt
     6481 cccaatcgct gtcctaattg tggcggcaat aaaatccgct actatggaac aggtactcaa
     6541 aaagtagaag aggaactccg agaactttat ccgcaagcta ggattttgcg tatggatgta
     6601 gataccactc gaagaaaagg ggcacacgaa cagattttac aaaaattcgg tgcaaaagaa
     6661 gcagatatat tattagggac acaaatgatt gctaaaggtt tggactttcc agaagtgaca
     6721 ttagttgggg tgttgaatgc tgatacctcc ctgaatttac cggattttcg ttctagtgag
     6781 catacattcc aattgcttac ccaagtttca ggacgagcag ggcgagcgga aaaagcaggc
     6841 gaagtcgtga tccaaacatt caatcctcaa cattacgcca tagaattggc taaaaaacaa
     6901 aattatgagc agttttatca gcaggaaatg catgtacggc atcgtggagg ctatcctcct
     6961 tattacttta ctgtgaaaat aaccgccagc catcctgaag aacaagtagc ggcaaagaaa
     7021 atctttcaga ttgccaatca gctaaaagaa gtgttaagcc ctcaaagctt gctattaggg
     7081 ccaacgccca gcatgatttt gagagtgaaa aaccgatatt attatcaact aatcatcaaa
     7141 tataaacatg aaccgaatct tcctcatgtt ttggatgaaa ttttgaatgg aagccagaaa
     7201 gaacagcgac aaggtctttt tgtggcaatt gacaacgaac cattaaactt tatttaagaa
     7261 agggccaaat tatgcgctat cctatattga tccaccctaa tgataaatta aaaagaactg
     7321 ctcagccaat cgatgtaatc acggacgaga caatcgcttt attagacaat ttatatgaga
     7381 caatgattgc gaatgacgga atagggatcg ctgctcctca agtaggacaa aacaagagaa
     7441 ttgctgtgat cgaagtagat gaaggcgaga aatttgaact gattaaccca gaaatcatcg
     7501 aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag tattccgcat gtctatggaa
     7561 cggtcaagcg agcagatgaa gtgaccgtgc gttattatga tcgagacgga gaagagatag
     7621 aagtgacggc ttttggctat ttagcccgtg ccttccagca tgaaatcgat cacttagacg
     7681 gtatcttgtt cattgagaaa atgatacagc agatcccgga agaagaactt gaagaatata
     7741 tggaggaaca tcttgatgac taagctagta tttatgggca cacctgcttt ttctgtgccg
     7801 attcttgaag ggctgataga atcaggttac gagatccagg cagtggtaac acagcccgac
     7861 cgtccagttg ggcggaaacg agtaattacg ccgacaccgg taaaagaagc ggctctgaag
     7921 cacggtatcc gtgtgctgca gccagagaaa atctccggtt cgccagaaat ggaagaaatc
     7981 attgaattgg tgcctgatgt gatcgtcact gcggcatttg gccaattctt gcctgaaaag
     8041 ttattgcaag taccaaaatt aggggcaatc aatgtccatg cttctttact tcctaaatat
     8101 cggggaggtg cgcctgttca ttatgccatc atgaatgggg agaaagaaac tggtgtaacg
     8161 atcatggaaa tgatcaaaaa aatggatgca ggcggaatct atgcgcaaga aagcatacca
     8221 atcactaaac aagatgatgt agggacgatg tttgaaaagc ttagcctttt aggaagaaag
     8281 ctattgctag aaacattgcc caatattttg gatggacaaa aaccagtacc tcaagacgag
     8341 tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag aagcaatcga ttggcataaa
     8401 actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc cttggccaat tgcattcacg
     8461 acctatgagg gcatacgctg gaaattatta tcggttgaag tattggcaga aactacagac
     8521 aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc tatggattgc ttgcggtaaa
     8581 cagaccgtct tagccatcaa tgaattacag ccagctggta aaggaaaaca ggccgtacat
     8641 gatttcctta acggaagcgg tcagcaagtt cagattggtc agcaggtgaa ataatgaaaa
     8701 aaatccctaa aaaaatgaaa aaatcagtac gatatatggc attactgaca ttagagcgag
     8761 tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga gatgatcaaa caaagcgagc
     8821 taaatcaaaa agatgtgggt ttatttacag aattgatcta tggtacgatt agccatcagc
     8881 gactattgga attttacgtt gcaccattga tcgctaaagc aaaaaaagtg gatgactggg
     8941 tcaagacgct tctttatctt tctgtatacc aattagaatt tttagataaa gtccctgcac
     9001 atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa aggaaatcct gggacaggga
     9061 aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg agcaccagat ctgcaaggca
     9121 ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct gcctctatgg atgactaaac
     9181 gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct cggtttatcg ctatatcagc
     9241 ctagccatgc tagtgcccga gtagatacac gccgactttc cagagatcag gccatcactg
     9301 ttttgcaaga agaagagatc acagctgaaa aaagccaagt cagtccatac ggtatagtag
     9361 cgaaaaaagg gcatttagct tctagctcat tgttccacga tggcgtcatg acgatccaag
     9421 atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga gcctgaacat catgtcttag
     9481 atgcctgtgc agcacccgga ggtaagacga cgcatattgc ttctttttta gatccgtcat
     9541 gcggtggaga agtggtcgct ttagatgtac acgaacacaa agtcaaactc attgaagata
     9601 atgcagcacg tctaggtgtc tcttctgctg tctacgctca aaagatggat gctagagaag
     9661 tcagtaaaca gttcgaagca gagcagtttg atcggatttt agtagacgcg ccttgttcag
     9721 gtcttgggct gcttcggcga aaaccagata ttcgttataa aaaacagccg gatgaattag
     9781 cgaatttacc aaaaatccaa ttggaaattt tagaaagctg cgcaccaact ttaaagtctt
     9841 ccggtattct gacttatagc acttgtacga tcttaaaaga agaaaaccaa gacgttgttt
     9901 ctgctttctt ggaaagacac cctgattttg agaggattga tgttttgacc aatcctatgc
     9961 tacaatcatc aatcgaagag aaaatgttga ctttgtatcc gcatcagttt tacacagatg
    10021 gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga aaaaatgcag attgaatatc
    10081 aatcagatgt aggaagacga cgtaacacaa atcaagacta tgccagtgta tttaccagcc
    10141 gttcaggtct caagcttgct gtattagcag acgggatggg tggtcatcgt gccggagaca
    10201 ttgccagtca gatggcggta acgaatctag gcgaagcttg ggaaaaacaa gaagctgttg
    10261 acgatgaaaa aatcgcccaa tggttcattc aatcgattca agaagaaaat atgctgattt
    10321 atcagcgtgg acaagaacag ccggaatata acggtatggg aacaacaatc gtagcggccg
    10381 ctctgtcaga tgactggttc acgattgcca atgttgggga tagcagagct tatttgattc
    10441 gtgaagataa aatcatgcaa ttgacggagg atcattcttt agtcaatgaa ctagtcaaat
    10501 caggagaaat cagcgaagaa atggcagtga accatccaag gaaaaatatt ttaacacgtt
    10561 ctgtcggcat gcctggaaca gtcgaagtag atgtttctac tcatctttgg caactaaaag
    10621 atcgactgct gttatgttca gatggcttga cgaacatgct gtctgaagaa gaaataggaa
    10681 cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac tgagctgatt gatcgtgcca
    10741 atgaagcagg cggagcggat aatatcaccg ttcttcttat cgaatataag gaggacagtg
    10801 tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa atcactggaa atatcggtag
    10861 cggtggtatg gcaaatgtat ttttggcgca cgatttaatc ttggatcgtg atgtagcagt
    10921 taaagtctta cgatttgact ttcaaaatga ccagactgcg attcgacgat ttcagagaga
    10981 agctttagca gcaacagaac tcgttcatcc aaacattgtt agtgtttacg atgttggtga
    11041 agaagacaac atgcagtatc tggtgatgga atacgtcaaa ggaatggact tgaaacgata
    11101 tatccaaaca cattatcctg ttccttatga aacagcagta aatattatgc agcagatcct
    11161 ttctgctatt tctttagctc ataaccatca aatcatccac cgagatttga aaccccaaaa
    11221 cgtactgatc gataatgaag gtgttgtaaa aatcacggat ttcgggattg ctatcgcttt
    11281 atctgaaaca tcgatcacgc agacgaatac gatgctagga tctgttcatt atctgtcccc
    11341 tgagcaggcc agaggaagta tggcaacgaa gcagtcagat atttatgctt tagggatcat
    11401 cctttatgag atgttgacgg gtagcgtacc gtttgatggt gaatctgctg taacaattgc
    11461 tttaaaacat tttcaagatg atcttccatc tataaaagca cttgatccaa atgtgccaca
    11521 agcactggaa aacgtgattt tacgggcaac ggctaaagaa cctgcagacc gttataagtc
    11581 agccgaggaa atgtctgatg atctttctac agtattatca ccagctcgtg caaacgaaga
    11641 aaaatggcag cctcatgtga tggataatga aacaaaagtt atcacacccc ttacggaaga
    11701 tgcaccaatg cctgattcat tcaaatctat gcctctgcca aaagacaagc cagaaaacga
    11761 aaccttacca gaggaggaag aagttctgcc tgatcctaaa aagaaaggac agaaaaaatg
    11821 gtggctgatc ctattagcta ttcttgctgt tttaggaatc ggaacagctg tattctttgc
    11881 ttctggcggg cgaggagaag ttgttgttcc tgatgtttct gatctttctg aagcttctgc
    11941 tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca aaaacagaag aaattgcaga
    12001 tgacaaaatc gaagaaggta atgtagtgaa gacagacccg gcagcgggaa cgacagtcaa
    12061 gcaaaaacgc gaagtcactc tgtatattag tagcggcacg aaaaaaatca aattagatga
    12121 ttacagtggt aaaagttatg aagatgccga agcaagtttg aaagaattag ggttttctgc
    12181 tgacttgatt caaaaagaag aggaattcag ttcagaggta gaaagcggaa tgatcatttc
    12241 gcaatctcca tcagaagata cagaagtcga tcctaaaaca gacacgatta ctttcacagt
    12301 cagcaaagga ccagaaacag tgactttgag tagtcatgta ggagaaaact acgaaactgt
    12361 tcgtaattgg cttgttacac aaggattcaa tatcaataat attagtgcta cttatgacta
    12421 tagcgacact gtaccatcag gttcgatcat cagtcaggat ccagcctcgg gtgaagttgt
    12481 agcagaagaa acttacatca attttgtcgt aagccaagga acagagccta aattgaatga
    12541 tatctccggc tatacaaaaa gcgaagcaca aagttattta gctagtgtcg gtgcagaata
    12601 tatcgggcat gagacttccg aatattcaaa cacggtggat aaagataaag tgattcgtac
    12661 agtaccaggg gcaggaacga caattaccaa aggaatggtt gtgaacgtaa tttactctaa
    12721 aggaccagat ccagcgtctt ctagttctga aaaagactct tcagacagtt ctagcagctc
    12781 aagtagctcc agtagcacaa gcaactcttc aaaagaagaa tcttccagct ccacaagttc
    12841 atcagctagc acctcagaaa gttcaaaata agtgctgaat aaaatatatc agaagggaat
    12901 gcgacattga tttgttgcat tctctttttt gtgtttctat ctcttttgat caattttcaa
    12961 gccatatact ttgagaagtt tgcatggaat aacacctact ttcctcttcg tatggtaaaa
    13021 taaacaaaaa cggaggtggc acttatcaaa ggacaaatca gaaaagcaat cagcggtttt
    13081 tactatgtat acgcaaacgg agagacctat caaacaagag gaagaggaaa tttccggaat
    13141 cggaaaatca cgccgcttgt cggagatcag gtgatctttg agagtgaaaa tcaaacagac
    13201 ggctatcttc tggaaatctt gccgagaaaa aatcaattag ttcgcccacc agtcacaaat
    13261 gttgatcttg gaatcattgt cacaagtatc gtagagcctg cattttctta taatctactt
    13321 gaccgctttc tagttacctt ggaatacgag tcgatcgaac cgattatttt tttgacaaag
    13381 accgacttgt cagacgaccc agaaaaaatc gaagagatca agcaaaccta tactaaaata
    13441 ggctatccgg tgattgtacc caatcatacg ggtgatacga cagaactgat ccgttacttc
    13501 ccagaacggc tatcggtctt tatgggtcag tctggagcag gaaaatcgac gctactgaat
    13561 caaatatcac cggaactgaa tttagcaaca ggagagattt ctgatgcact tggaagagga
    13621 cggcatacaa cacgtcatgt ggaactgatt cctatctatg gcgggttagt agcagataca
    13681 ccaggattta gctcgattga ctttttgaca attgaagcag gagaactgcc gagacaattt
    13741 cctgaatttg tagaagcttc tgtccattgc aaatttagag agtgcatgca tgctaaagaa
    13801 cctggctgcg aagtaaaaca tagagtagaa acaggtggta tcgcacagac acgttacgac
    13861 aattatttgc aattcttaca agaaatcgaa aaaagaaaac caatgtatgc aaaaaaagaa
    13921 ggtaaacgaa aataggagga aacaagaatg aagatagcac catccatttt gagtgcagat
    13981 tttgccaatt tacaaagaga cattgagtta gtggaaaaag gaggcgcaga ttacatccac
    14041 gtagatgtaa tggatggcca atttgtccca aatatcacat tagggccaaa tattgttcag
    14101 gcaatccgtc ctgttactaa attaccgtta gacgtccatc tgatgatcgt tgatccagaa
    14161 aactacattc cagcatttgc aaaagctgga gcagatatca ttactgtcca cgtcgaagca
    14221 actcctcata ttcatcgggc acttcaaatg acaaaaagct taggagttaa agctggagta
    14281 gttatcaatc caggaacacc agtctccatg atcaaacatg ttttgccttt ggctgatcaa
    14341 gtactagtca tgactgtcaa tcctgggttc ggcggacaaa gctttattga agaaacagta
    14401 gaaaaaatta cggaactcgc tgatctacgt agagagaaca attggactta tgcaatcgaa
    14461 gtagatggcg gaatcgttcc agaaacagca aaaatctgta aagatgcagg ggcggatgtc
    14521 ttcgttgcag gatcatatat ctatggagcc gaaaatccag tcggacaaat cgaacgatta
    14581 aaagaggcat tagactaaat gaaaatttta ttagctgctg gaggtaatcc gcaaaactgg
    14641 ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga tagaccgtgg tgcgctgtac
    14701 ctgcttcaag caggctaccc agtagatctg gcagtgggcg attttgattc attgtcgaac
    14761 gaagagcaga aggaagtatt tgcaaaagcc aaagagtata cccgttcccc tgcagaaaaa
    14821 gacgatacag atacacagtt aggtatctta aaaacatttg aacgttaccc gcaagcggaa
    14881 gtcactttga ttggagctac gggcggaaga ttggatcatc ttttagccaa tctctggtta
    14941 ggtatggaac ctcgcttcaa accctttgcc catcagctta ttatcagaga ttgtcaaaat
    15001 tatttgtctt attatttgcc tggagagcat atagtaagac agataccaga tatgaagtat
    15061 ttagcttatt gctgcttgac tcccgtctcg aatcttacat taaaaggcag caagtatgta
    15121 ttggaaaata aagaagtaga atatccgaca tcttatgcaa gtaacgaatt tctttcagat
    15181 accgcagaat tttctttttc agatggtatg attgcagtta ttcaaagcaa agattgatcc
    15241 ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga cagaatctat tatttgctaa
    15301 aaaaaagctt aagaggagca acttaccttt tgtttcacgg catttagtga taaaattgaa
    15361 cacaagagtc taattaattc gaggtgtctt tgtgaagaag ttcaatccta ataaaaatat
    15421 tattattacc ttggttattg ttatcatcgt ggtgacgata ctaagtgtga ccgctgcgca
    15481 gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc gttaatgaca gtgttgcaat
    15541 ggttgataaa acaatctctg ccccagtacg gtgggtacga aatggagtag attctgtcca
    15601 taatcttttt tcaacttatg ccgaaaatga gcggctaaaa gaaaaaatcg atagctatga
    15661 tgacgtttca cagcaaaaca agaattacga gagagaaatc gaagcgttga aaaaagaact
    15721 tgagttgaat cagacgctga ctagttacga aaaagtaacg gcaaatgtca ttactcgttc
    15781 gcctgacagt tggcaggaca tgctgatcgt agacaagggt tctaaagacg gtatcgaagc
    15841 aaatatggca gtcatgtcgc aaaaaggatt gattggccga gtgatcgagg tcaatacggc
    15901 ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc tccaatcatt ttccagtacg
    15961 ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa aactatgatg aaaagctcca
    16021 tgccttagtg gtgacccaat taactggtga tacggatatc aaagaagggg atgttgtcca
    16081 gacatccggt cttggaggga attctccagc taacttgccg atcggtacgg ttattaaaac
    16141 gaaaccagat agttatgggc tggatcggga agtttatgtg aaaccttatg cagaaatgta
    16201 tgacgtgtca gttgtgacga ttgtccaacg gctagcggag gattagtcca tgttgaagaa
    16261 agaaacaatg aagtactatc tgccaatcgt tttgttcttt ttgatgttga tagatggtca
    16321 tttaacaaga atgctagggg agtggtcgaa aggcacctat atgtcaaatg cccactttct
    16381 gatattggca ttattatgtt gcagtatggc gtttgaaaaa cgttatttac tgattaccac
    16441 gattgttctc ggggctatct atgacgctta ctatattggc gttatcggta tctatgcagt
    16501 agctctccct ttaattgtat ggttgatgta tgtaatgaaa gacgttatcc atgtcaacat
    16561 ctttactgaa tttttcagta tgatcatctt tgtcacgggt tatgaattgt ttacgatggt
    16621 ggtccagttg atttttaaat tagcagtagt aaataacacg tattttatta caaggttttt
    16681 aggacctaca ctgctgttga acatgattat atttgtatta ttcatttttc cctttaagaa
    16741 attattcagt gatgaataat ttcttttttt atttttaggt cgaaagcgaa aaagaatcgt
    16801 tgttatctta agaaatgcta gacttaacgc ggctttttca tgatcatttt ctgaaacgaa
    16861 cagttttgtc atttttaaaa gttgttttga aacatttttg taacataact attatatcgt
    16921 tgacacactg ctatggtaca atttttatat cgttaagaaa gacttaaata tatttagata
    16981 tttattaaat taagccagtc ggaggaatga agagtgaaaa agagtttaat atcagcagta
    17041 atggtatgtt caatgacctt gactgccgta gcatcaccga tcgctgccgc ggcggacgat
    17101 tttgattctc agatacaaca gcaagatcaa aagattgcag acttgaaaaa tcaacaagca
    17161 gatgcacaat cacaaatcga tgccttagaa agccaagttt ctgaaatcaa cacacaagca
    17221 caagacctat tagcaaaaca agatacattg catcaagaat ctgcacaatt agtaaaagac
    17281 attgcagatt tacaagaacg tattgaaaaa cgtgaagata caatccaaaa acaagcacgc
    17341 gaagcacaag taagcaacac aagctcaaac tacatcgatg ctgtattgaa tgctgattca
    17401 ttagcagatg ctattggacg cgttcaagca atgacaacga tggtaaaagc aaacaacgac
    17461 ttgatggaac aacaaaaaca agataaaaaa gctgtagaag acaaaaaagc tgaaaacgat
    17521 gctaaactaa aagaattagc tgaaaaccaa gctgctttgg aatctcaaaa aggcgatctt
    17581 ttatctaaac aagctgattt gaacgtactg aaaacttcat tagctgctga acaagcaact
    17641 gctgaagaca agaaagctga cttgaaccgt caaaaagcag aagctgaagc agagcaagct
    17701 cgtattcgtg aacaacaacg tctagcagaa caagctcgtc aacaagctgc acaagaaaaa
    17761 gctgaaaaag aagcacgcga acaagcagag gctgaagcac aagctactca agcttcatct
    17821 actgcacaaa gctcagcaac agaagaatca tcagctacac aatcttcaat gactgaagaa
    17881 tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa caacacctga atcttcaaca
    17941 gaagaatcaa cagcgcctga atcttcagca acagaagaat caacaacagc gcctgaatct
    18001 tcagcaacag aagaatcaac aacagtgcct gaatcttcag caacagaaga atcaacaaca
    18061 gtgcctgaat cttcaacaac agaagaatca acaacacctg cgcctacaac accatcaaca
    18121 gatcaaagtg ttgatacagg aaacggcaca ggaagtagta ctccggctcc aacgccaaca
    18181 ccaacacctg aacaaccaaa acctgtaaca cctgctccag ctccttctgg aagtgtgaat
    18241 ggtgcagcaa tcgtagcaga agcttacaaa tatattggta ctccatatgt ttggggcggt
    18301 aaagatccaa gtggctttga ctgctcaggg ttcacacgct atgtttacat gcaagtaact
    18361 ggtcgtgaca tcggtggttg gacagttcct caagaatcag ctggaacaaa aatttcagtt
    18421 tctcaagcaa aagctggaga cttgttattc tgggggtcac aaggcggaac ttaccacgta
    18481 gcgattgctt taggcggagg acaatatatc catgctcctc aaccaggcga aagcgtaaaa
    18541 gttggttcag ttcaatggtt tgcccctgac tttgctgtca gcatgtaaga atgattaatc
    18601 aaaaagagtt agcccattct ggactaactc tttttttatt gtgataaaat agaaatcaga
    18661 taaattcttc tgtcaattcc atgaacgttt ggatatcttc agtcaccatg tcgatacctg
    18721 cttgccaaaa atctggttgg gtaagatcga cattcaaatg tttcttcgca agatcttccg
    18781 tcgtcattga ggcagtatcg cgtagtaaat ggatatattc ttcttcaaaa tttgctccct
    18841 tttggttggc gtaagcatag atgcccaaac taaagaggta gccaaatgtg tatgggaagt
    18901 tataaaacgg tacgtcatca ataaagaaat gcaattttgc agcccagaaa tatggatggt
    18961 aagtagctaa agctccttga tagccttctt tttgcgcctc aaccatcatc tctgtgatct
    19021 tatcttctga gactaatcct tcttgccgtg cttcatagaa tcgattttca aaaatgaatc
    19081 gggaatggat attcatgaac atagctaaag cattttgcag ttttgtatct agcaaattga
    19141 ttttttcttc ttttgttttt gcgactttta atgtagcatc ggctacgatc agttctgcga
    19201 atgtacttgc tgtttctgcg acattcatgg catattcgcg atttaaagaa ggtaaatccc
    19261 acatcgtgct gctatggaaa gcatgtccta gttcatgagc aagtgtcgca acctcattaa
    19321 cagaattact gtaggtcata aagatccttg attcttttgt ttctggtaac tcagtacaat
    19381 aaccaccagg acgtttccca ggacgatctt ctgcttcgat ccagctcttt tcaaaagcag
    19441 attgggcaaa tttagccatt tttggactga atttgttaaa gttttcgata ataaatgctg
    19501 ctgcttcgtc aaaactaaag gttttttctt tcaaatcgcc taaaataata ggtgcatctt
    19561 gatcttgcca ttccattttt tctttaccga aaagattagc ttttctagtc aagtaattaa
    19621 caaggggttg tttgtttttc tggattgtat cccacatgac agacaatgtt tctttcttca
    19681 accgattgta ctctaatggt ttttgtagaa aatctgtgac accatgaagt tcatagtcag
    19741 aaagtctaaa accatctaaa tgattcaaag tatccgtgaa gaggggtgcc ttttctttcc
    19801 aggcttcttc ccatgctgcg aaaagtgttt ctctgacttt tggatctgga tcgcccatca
    19861 tcttattgaa ggcttgtcca gcagataatt cgactacttg tccatcttgt tcgaagggaa
    19921 tcgaaatgct ggctacaatc gtatcataat gactgctcca agcatttaga ccatctaaag
    19981 aaagcgtatt tataatgttt tcttcagctt ctgataataa ttgtgagcca tcacgacgaa
    20041 tctcgtttaa acgaaaagca attgtttcaa acgaagattg agaaagcatc tgattccaat
    20101 cattgtcaga tatttctgca aatttctttg acaatacagt ttcggctgat tgcagacgag
    20161 gcagcattgc gtaaagcttg cctgataaga cctttgcatc tgaatcattg acattggcag
    20221 aaagcaatgc agtaatatag ctgctgcatt gggtaaaacc attcgtaatc gtttcttgca
    20281 attgaagaat agcgtttaac tgctctgcat tgtcagaact aggtgaccat tttgttacgc
    20341 ggtgataata ctcgttcatt tggtcttcca attgcttcat ccgttggttc aaagcatcag
    20401 aatgactgcc tccggaaaaa atagaatcta gattccaatt taatgaataa ctcatgaaaa
    20461 aactcccttc atattgtaat acaaaattaa gtatagcact tttttatgga agaagtctct
    20521 atgaaaatga ttgttgtttc ttcaaaaaaa gtctaagata aaatacagag gtgacttatg
    20581 aattcttcaa aaaaacaaat gaaaaacgga aatatttatt tagtaatcgc actgatagtc
    20641 gttataatct tattttacag ttcttctcaa acttacggac agcaatcaca agtttcgcga
    20701 ttagaaagct ggctgcctaa tcagccattt aaagacacat tagcgggtat acgttttaat
    20761 tatggtggaa gtatggtgag tatcgaccat ctgggctacg ccaaattttt agagttcttt
    20821 ttaaggaagg gcgcacattt cggtacctat tttattttgg gaggcagctt gtttttaggt
    20881 gttttcccca agctaaaaat ttggtggctg acagcgatcc ttgcttggct ttcagcaaca
    20941 gggtacgcag gtttggacga attccatcag atgatgaccg gtggacgaac acctatgttc
    21001 caagatgtca tgttggattc gatcggtgct ctgactgctg tgatattatg tgtgcttttt
    21061 gcggcattca agaagcgttt atagattggc tgtggctttg tttttttgtg aaatagtact
    21121 agaatagaag tgtattctca aaagaacaag ggaggaaaaa tcatgtcagg acatagtaaa
    21181 tggcataata tccaaggaag aaaaaatgcc caagatgcaa aacgagggaa agtctttcag
    21241 aaactttctc gtgaaattta tatggcagct aaagcaggcg gtcctgatcc atctatgaat
    21301 ccagctttgc ggcttgttgt ggacaaagca aaagctgcaa atatgcctaa cgacaatgtg
    21361 gaacgggcga ttaaaaaggc tacgagtgcc gtagatgaaa cgaattacga tgaaatcacg
    21421 tatgaaggat atggtccggg tggagtagga atcttagtcc atgcattgac agataaccgt
    21481 aatcggacag cgacaaatgt ccgagtagct tttactcgaa acggtggatc actgggagaa
    21541 acgggttcag tcagttactt gtttgaccgc aaaggatata tcgctattga acgcgatggg
    21601 ttgactgttg atgaagagac gatgtttgag gatgtattag aggcgggggc agaagatctg
    21661 caaacttctc ctgaagtatt cgagatttat actgcaccag aagattttgc tgctgtacga
    21721 gatgagttgg aaaaagagtt cactttggcg caagcagaat tgacgatgat tccacaaacg
    21781 acgattgatt taaatgagga acaaaaagag caattggaac gtctagtaga taagctagaa
    21841 gacgatgatg atgtttcaga agtctttact gctgctgaaa tgtaagtagc acattgttag
    21901 ataataaaaa gaaagcacct gtaccaatcg tacgggtgct ttctttttat atattatttt
    21961 tgttagaaga ccaatacagg cgttcctttt tcgaccatcc cgaataattc cttcattaca
    22021 cttggtgggg tgttgatgca gccgtgtgaa ccgcgggttt tccataagtc tccaccgtat
    22081 tcaggttgcc agtctgaatc gtggatacca acacctgtcc agtcaatcgg catccagtag
    22141 tttaccggac tctcgtaagg tgttccatcg tcatttgttc cttttaaagt tgcatcttct
    22201 tctttattcc aaacatagaa gactccagca ggtgtaggtg tagttggctt tccggaaacg
    22261 atatctgttt caagggccac ttttccgtct ttgtaatacc acatatgctg gttctccaga
    22321 tcaacttcga tatacgtatc ttcgatcaat ggatgatcag cggttgtgcc accttgcaca
    22381 atgggtgaac gagtaaaatc ttggcctgcc agaatcgctt ttttcaatgc ttctgtctca
    22441 ctgtctgttt ggatcgtcca actgtaagtg ccaactggga ccgtaacttc tcctcttttt
    22501 gtacttttaa attttgtgtc attcgtactt gtgttatact ttgtgcctaa atctgttaca
    22561 tattgacgta cttgttctgt atctaaatcg acttttccat cattgtaggt cagccaactc
    22621 ataatatcac ttgatgggat ctggaaagtc tcaccgttta tactgtaagt agcttttaca
    22681 tttgcgatag cattcatgga tgcgagttgt tctttcagtg agctgtcttc agatgttact
    22741 tttggttttt ctttgaattc agtcaattca attgtatctt taccagagtt taccgcactt
    22801 tttaattgtt tgaccgcagc atcgacgtcg atcgtatttc cattgacttc aggcttgata
    22861 acgaaagagt cacctgcttt ttcgatcgtt gcatcagtag taggtgtccg gtctttgttc
    22921 aaatcagtca gcttagttgt caatgtctga acggtagtgt ctagtttttg gtggttttga
    22981 ctgacaggat ctatttcttg tttttctgcc gctgagacat aagtcattcc ccaagtccat
    23041 ccgttttgtt catttaggat cggagaaagc tccgaagtga aatcatcttt gtagccgagc
    23101 tcggattttg gtacttcctg ccagactttt ccatcttctt taatagccag ttttttatca
    23161 gaataagctt cttttaattt ttcatttgct ttttcgactg ttagattgct gacatttatt
    23221 ccatttattt ctgtattcgg caaaaaatgc tttgtgtagt atgtactgcg ataggtataa
    23281 aagctgatca gcactaagag aacaccaaga actgaaagca aagcaacatg tttcgatttt
    23341 tttcgatgcg aagatcttgt catatcgaca actccttaag taaaagttta gggtggaacg
    23401 gtctcatgat agcataattt agaggaaaag acgaaggtta tctctacttt atgaaaaaat
    23461 ttagaaacca taagggtgat aaaataaaaa aacggaaaac accgaataga tgttttccgt
    23521 catgttcaaa gattattcag cgtctagttt agcaaaagct tcatctagaa tttcttttaa
    23581 ttgcttagca gaagcagcca tacggtcttg ctcgctgtca cttaaaggaa tttcgattac
    23641 tttttggata ccttgacggt tgataacagc aggtgcacca atgtagatgt cgttcaatcc
    23701 atattcgcca ttcatgaata ctgataaagg aagaacagca ttttcatcat ctaagatggc
    23761 acgagtgata cgtgctaatg ctactgcgat accatagaaa gtagcgcctt ttttctcgat
    23821 aattgtgtaa gcagcgtcac gtacgccaaa gaataggtta accatagctt cttcatcgat
    23881 ttctgggttg ttttttaccc attcatagat ttgcaagccg gcaacatttg catgtgacca
    23941 aactgggaat tctgaatctc cgtgttcacc taagatgtaa gcatggacgt tacgtgcatc
    24001 aacgtcaact aattccgcaa gtgcttgacg gaaacgagct gagtcaagag aggtacctga
    24061 accgattacg cgttctttag ggaaacctga gaatttccaa gttgagtaag tcaaaatatc
    24121 tactgggtta gcggcaacta aaaagatacc gttaaatcct gattcaacga tttgtgtgac
    24181 aacatcgcgg ttgattttta ggtttttgtt tactaagtct aaacgagttt cacctggttt
    24241 ttgtggtgcg ccagctgtaa taacaactaa gtcagcatca tgtgcgtctg cgtaagtagc
    24301 agaatagatt tttttaggtg aagtaaatgc caaagcgtga gacaagtcaa ttgcgtctcc
    24361 ttcagtttta gcagtattga tatccacaat acctacttct tgagcgatat tttgagtaac
    24421 tagagcaaag gcatagctag aacctacggc gccgtctcca acaagaataa ctttttgatg
    24481 atcttttttt tctgcagctg cagtcattta gtatcaatcc ttccattgat ttgttttttc
    24541 ccatgcttat gctaacattt ctcaagcccc atgtcacgct atttggctat aaaatataac
    24601 agataacaag gttgtattat atgtaaaagc ttacatg
//
LOCUS       DAXWLH010000025        24579 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXWLH010000025 DAXWLH010000000
VERSION     DAXWLH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.025"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(153..551)
                     /locus_tag="ACGE36_000735"
     CDS             complement(153..551)
                     /locus_tag="ACGE36_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFF8383154.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACGE36_000736"
     CDS             complement(548..1321)
                     /locus_tag="ACGE36_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8383155.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACGE36_000737"
     CDS             complement(1318..2001)
                     /locus_tag="ACGE36_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8383156.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACGE36_000738"
     CDS             complement(2168..3055)
                     /locus_tag="ACGE36_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383157.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACGE36_000739"
     CDS             complement(3052..3921)
                     /locus_tag="ACGE36_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383158.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACGE36_000740"
     CDS             complement(4058..5344)
                     /locus_tag="ACGE36_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8383159.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACGE36_000741"
     CDS             5617..6195
                     /locus_tag="ACGE36_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8383160.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACGE36_000742"
     CDS             6195..7124
                     /locus_tag="ACGE36_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383161.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACGE36_000743"
     CDS             7121..8857
                     /locus_tag="ACGE36_000743"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8383162.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE36_000744"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE36_000744"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFF8383163.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACGE36_000745"
     CDS             complement(9753..10262)
                     /locus_tag="ACGE36_000745"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFF8383164.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE36_000746"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE36_000746"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFF8383165.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACGE36_000747"
     CDS             complement(11563..12654)
                     /locus_tag="ACGE36_000747"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFF8383166.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE36_000748"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE36_000748"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFF8383167.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE36_000749"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE36_000749"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFF8383168.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE36_000750"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE36_000750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFF8383169.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="ACGE36_000751"
     CDS             complement(16617..17591)
                     /locus_tag="ACGE36_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFF8383170.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="ACGE36_000752"
     CDS             complement(17572..19455)
                     /locus_tag="ACGE36_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFF8383171.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="ACGE36_000753"
     CDS             complement(19473..21698)
                     /locus_tag="ACGE36_000753"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8383172.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="ACGE36_000754"
     CDS             21813..22847
                     /locus_tag="ACGE36_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8383173.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="ACGE36_000755"
     CDS             complement(22914..23927)
                     /locus_tag="ACGE36_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8383174.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="ACGE36_000756"
     CDS             complement(24005..24193)
                     /locus_tag="ACGE36_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFF8383175.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7065 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaaaa
//
LOCUS       DAXWLH010000026        24528 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXWLH010000026 DAXWLH010000000
VERSION     DAXWLH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.026"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..366
                     /locus_tag="ACGE36_000757"
     CDS             <1..366
                     /locus_tag="ACGE36_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8383176.1"
                     /translation="ISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTALGLPAVLVPS
                     PYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQKQMCRASKEQ
                     GIPDASKRLYDLVKQIIKK"
     gene            381..1601
                     /locus_tag="ACGE36_000758"
     CDS             381..1601
                     /locus_tag="ACGE36_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFF8383177.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            1757..3082
                     /gene="ftsA"
                     /locus_tag="ACGE36_000759"
     CDS             1757..3082
                     /gene="ftsA"
                     /locus_tag="ACGE36_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFF8383178.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            3105..4346
                     /gene="ftsZ"
                     /locus_tag="ACGE36_000760"
     CDS             3105..4346
                     /gene="ftsZ"
                     /locus_tag="ACGE36_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFF8383179.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            4363..5040
                     /locus_tag="ACGE36_000761"
     CDS             4363..5040
                     /locus_tag="ACGE36_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFF8383180.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            5054..5653
                     /locus_tag="ACGE36_000762"
     CDS             5054..5653
                     /locus_tag="ACGE36_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFF8383181.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            5734..5955
                     /locus_tag="ACGE36_000763"
     CDS             5734..5955
                     /locus_tag="ACGE36_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFF8383182.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            6004..6786
                     /locus_tag="ACGE36_000764"
     CDS             6004..6786
                     /locus_tag="ACGE36_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFF8383183.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            6807..7514
                     /locus_tag="ACGE36_000765"
     CDS             6807..7514
                     /locus_tag="ACGE36_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFF8383184.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            7776..10574
                     /gene="ileS"
                     /locus_tag="ACGE36_000766"
     CDS             7776..10574
                     /gene="ileS"
                     /locus_tag="ACGE36_000766"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFF8383185.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(10623..12149)
                     /gene="zwf"
                     /locus_tag="ACGE36_000767"
     CDS             complement(10623..12149)
                     /gene="zwf"
                     /locus_tag="ACGE36_000767"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFF8383186.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(12164..12811)
                     /locus_tag="ACGE36_000768"
     CDS             complement(12164..12811)
                     /locus_tag="ACGE36_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8383187.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(12995..13324)
                     /locus_tag="ACGE36_000769"
     CDS             complement(12995..13324)
                     /locus_tag="ACGE36_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFF8383188.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            13501..14229
                     /gene="sfsA"
                     /locus_tag="ACGE36_000770"
     CDS             13501..14229
                     /gene="sfsA"
                     /locus_tag="ACGE36_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFF8383189.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            14245..15258
                     /locus_tag="ACGE36_000771"
     CDS             14245..15258
                     /locus_tag="ACGE36_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8383190.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            15258..16535
                     /locus_tag="ACGE36_000772"
     CDS             15258..16535
                     /locus_tag="ACGE36_000772"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8383191.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            16598..19300
                     /locus_tag="ACGE36_000773"
     CDS             16598..19300
                     /locus_tag="ACGE36_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8383192.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            19452..19769
                     /locus_tag="ACGE36_000774"
     CDS             19452..19769
                     /locus_tag="ACGE36_000774"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8383193.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            19799..20119
                     /locus_tag="ACGE36_000775"
     CDS             19799..20119
                     /locus_tag="ACGE36_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8383194.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            20227..21687
                     /locus_tag="ACGE36_000776"
     CDS             20227..21687
                     /locus_tag="ACGE36_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8383195.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            21755..21976
                     /locus_tag="ACGE36_000777"
     CDS             21755..21976
                     /locus_tag="ACGE36_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383196.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            22007..22189
                     /locus_tag="ACGE36_000778"
     CDS             22007..22189
                     /locus_tag="ACGE36_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFF8383197.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            22189..22602
                     /locus_tag="ACGE36_000779"
     CDS             22189..22602
                     /locus_tag="ACGE36_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFF8383198.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            22725..23906
                     /locus_tag="ACGE36_000780"
     CDS             22725..23906
                     /locus_tag="ACGE36_000780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383199.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(23977..24150)
                     /locus_tag="ACGE36_000781"
     CDS             complement(23977..24150)
                     /locus_tag="ACGE36_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383200.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTPKTKE
                     NKKFKFTKKEDLL"
     gene            24304..24377
                     /locus_tag="ACGE36_000782"
     tRNA            24304..24377
                     /locus_tag="ACGE36_000782"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24338..24340,aa:Arg,seq:cct)
     gene            complement(24446..>24528)
                     /locus_tag="ACGE36_000783"
     CDS             complement(24446..>24528)
                     /locus_tag="ACGE36_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFF8383201.1"
                     /translation="HIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         8164 a         4241 c         5181 g         6942 t
ORIGIN      
        1 attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag tgatctgctg
       61 gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt accggcagtg
      121 ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat gagtcttgtt
      181 cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc actaagtcag
      241 acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa
      301 gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa
      361 aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt
      421 cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat ctggcagaac
      481 aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca cccctttcag
      541 attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag tccgagaaaa
      601 aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat aacggttctt
      661 ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt cgtcggttag
      721 ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat gtttctcctt
      781 atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc caagaaatca
      841 tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt catcgatcta
      901 cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat atacatttca
      961 aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct ttgatcgcaa
     1021 aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa aaagtagcaa
     1081 atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa atcaaagaat
     1141 taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct gaaatcaagt
     1201 acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac ggcaatcaag
     1261 ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag
     1321 atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat ccttacggta
     1381 gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt gaagaaaatg
     1441 cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa acacctggat
     1501 cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt caaatagaag
     1561 ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt
     1621 aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt gaatatatct
     1681 attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga atagtaagta
     1741 ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata ttggaacgac
     1801 atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta ttggagtagg
     1861 aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata aaacggtcca
     1921 agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt
     1981 aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa tgattgcagt
     2041 taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt cagctgccct
     2101 tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag attttactgt
     2161 cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat tagaaatgta
     2221 cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat gcgtggaaaa
     2281 tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg aatcaattct
     2341 ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg gccaaacaac
     2401 aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag aaggtggcga
     2461 atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg cagaagcgtt
     2521 gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag aatttccagt
     2581 agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt cagaaattat
     2641 ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc aaatcgaagc
     2701 attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc ctggtgttgt
     2761 ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg
     2821 gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag caaacttgag
     2881 tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat
     2941 ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg aagaaacaat
     3001 ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa tcaagggttt
     3061 ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa ttctcaattg
     3121 ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa
     3181 atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca
     3241 cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta ggccccaaat
     3301 atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa
     3361 gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc actgcaggaa
     3421 tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg
     3481 gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc
     3541 gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta ttaattatct
     3601 caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa gctttccgtg
     3661 aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc acagcgccag
     3721 gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat
     3781 tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa
     3841 tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt cttaacatca
     3901 caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg
     3961 cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa atgggcgatg
     4021 aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta
     4081 gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta ttagatatgg
     4141 atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg gatatcagac
     4201 gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc gaaaaaaaag
     4261 aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc
     4321 caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc tgacaacttg
     4381 cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa
     4441 gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga attggttaga
     4501 gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa gaaacaagat
     4561 ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg cagaaaggta
     4621 aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag attagcagaa
     4681 gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt
     4741 gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga atctttagct
     4801 gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga agcttctgat
     4861 gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa
     4921 ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga ctttcaaatt
     4981 gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa agacgaataa
     5041 aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt tcggattagc
     5101 tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg ctgtacagcc
     5161 aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg cgaatactca
     5221 tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc agccggctga
     5281 aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga aagaaagaac
     5341 agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga
     5401 accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg gagaagcaac
     5461 cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg attttctaac
     5521 aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga tttttctttg
     5581 tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag atgcgaattt
     5641 gtttgatctg taagaggagg aactacgatt tatactattg cactattact tcatttactt
     5701 caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct ctcttggttt
     5761 ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga accgtatatc
     5821 agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat tttaggggca
     5881 atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg gataactgtc
     5941 catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata ggaggggaaa
     6001 attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt tatcgattcg
     6061 gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac cgaatttctt
     6121 gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga tttgcgtttc
     6181 atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt ccccgattat
     6241 tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta tcctagcaaa
     6301 tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg gatcaaacgc
     6361 tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat tgcaaatgag
     6421 gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt acgcttggaa
     6481 ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga aagaggaaca
     6541 gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc cagacagcgg
     6601 tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa tacaagacct
     6661 gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag agtccagatc
     6721 caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt
     6781 aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc caaaataaga
     6841 cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc ttagatttag
     6901 ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac
     6961 attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac caatcaatca
     7021 tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga
     7081 tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa aaacgtgcac
     7141 atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat gcaacagaaa
     7201 aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga gtattccatc
     7261 aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg gaatgggatg
     7321 aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc aaagaagttt
     7381 tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag
     7441 aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg cctggtgaaa
     7501 atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg atgagagtgg
     7561 aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct tttccgaaat
     7621 tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca ctagatagat
     7681 gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac atgctttttt
     7741 gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat
     7801 taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa
     7861 aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta
     7921 gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta ggacattcat
     7981 tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc tatcgtgcac
     8041 catatgttcc aggatgggat acacatggat tgccgattga acaagtattg gctaataaag
     8101 gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt gaatatgcac
     8161 tttcccaagt agataagcaa cgagcagatt ttaaacgcct aggagttgca ggagattggg
     8221 aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc gtatttggta
     8281 aaatggctga aaaaagctat atttacaaag gattgaaacc aatttactgg tctccgtcaa
     8341 gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct ccatctattt
     8401 ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca gcttttgtca
     8461 tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta aatccaaaat
     8521 ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag
     8581 aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa tttgctggac
     8641 aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca cttgtgattt
     8701 taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct ccaggacatg
     8761 gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca cctgtagact
     8821 caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat gacaaagcaa
     8881 atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta gatttcttta
     8941 ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg gcaactccac
     9001 aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta gaaaaagtag
     9061 agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac cgcggcgatt
     9121 gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat gctgaaaatg
     9181 gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt gctgaacatg
     9241 gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga tttacccacc
     9301 caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc tggtttgatt
     9361 ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc ccagcagata
     9421 tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc acgactagtg
     9481 tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt gctttagatg
     9541 gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa aaagtgatca
     9601 aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat gaagcagatg
     9661 ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa atcagaaata
     9721 cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat actgtttcgt
     9781 acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa gtaatcaaag
     9841 aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt acagttatga
     9901 acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat gtagtttata
     9961 ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat caaacagctg
    10021 tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa atctggtcat
    10081 tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat gaagagtttg
    10141 caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt gataatgtct
    10201 taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg gaagcaaaac
    10261 taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat gcagatattg
    10321 cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat gaacaagtgc
    10381 cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca gacggggaaa
    10441 cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc
    10501 tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta gctgaaggat
    10561 tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac ccccttttgt
    10621 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
    10681 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
    10741 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
    10801 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
    10861 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
    10921 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
    10981 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
    11041 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
    11101 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
    11161 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
    11221 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
    11281 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
    11341 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
    11401 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
    11461 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
    11521 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
    11581 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
    11641 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
    11701 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
    11761 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
    11821 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
    11881 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
    11941 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
    12001 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
    12061 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
    12121 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
    12181 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
    12241 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
    12301 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
    12361 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
    12421 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
    12481 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
    12541 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
    12601 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
    12661 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
    12721 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
    12781 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
    12841 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
    12901 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
    12961 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
    13021 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
    13081 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
    13141 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
    13201 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
    13261 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
    13321 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
    13381 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
    13441 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
    13501 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
    13561 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
    13621 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
    13681 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
    13741 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
    13801 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
    13861 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
    13921 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
    13981 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
    14041 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
    14101 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
    14161 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
    14221 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
    14281 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
    14341 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
    14401 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
    14461 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
    14521 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
    14581 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
    14641 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
    14701 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
    14761 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
    14821 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
    14881 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
    14941 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
    15001 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
    15061 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
    15121 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
    15181 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
    15241 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
    15301 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
    15361 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
    15421 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
    15481 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
    15541 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
    15601 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
    15661 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
    15721 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
    15781 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
    15841 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
    15901 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
    15961 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
    16021 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
    16081 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
    16141 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
    16201 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
    16261 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
    16321 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
    16381 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
    16441 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
    16501 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
    16561 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
    16621 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
    16681 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
    16741 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
    16801 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
    16861 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
    16921 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
    16981 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
    17041 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
    17101 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
    17161 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
    17221 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
    17281 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
    17341 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
    17401 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
    17461 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
    17521 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
    17581 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
    17641 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
    17701 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
    17761 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
    17821 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
    17881 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
    17941 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
    18001 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
    18061 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
    18121 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
    18181 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
    18241 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
    18301 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
    18361 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
    18421 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
    18481 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
    18541 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
    18601 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
    18661 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
    18721 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
    18781 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
    18841 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
    18901 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
    18961 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
    19021 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
    19081 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
    19141 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
    19201 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
    19261 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
    19321 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
    19381 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
    19441 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
    19501 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
    19561 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
    19621 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
    19681 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
    19741 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
    19801 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
    19861 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
    19921 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
    19981 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
    20041 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
    20101 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
    20161 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
    20221 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
    20281 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
    20341 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
    20401 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
    20461 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
    20521 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
    20581 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    20641 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    20701 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    20761 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    20821 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    20881 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    20941 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    21001 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    21061 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    21121 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    21181 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    21241 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    21301 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    21361 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    21421 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    21481 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    21541 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    21601 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    21661 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    21721 gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca
    21781 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    21841 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    21901 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    21961 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    22021 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    22081 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    22141 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    22201 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    22261 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    22321 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    22381 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    22441 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    22501 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    22561 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    22621 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    22681 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    22741 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    22801 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    22861 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    22921 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    22981 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    23041 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    23101 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    23161 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    23221 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    23281 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    23341 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    23401 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    23461 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    23521 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    23581 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    23641 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    23701 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    23761 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    23821 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    23881 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    23941 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    24001 aaatttgaat tttttgtttt cttttgtttt aggtgtttta gggttttctt ttttgtggtc
    24061 ttggattgct tctttcgtaa tcttttcccg atttttttta gatgaatcta agcgttctgc
    24121 gtatactttt tcttttggct tatcattcat tggaaaggac ctccctacat tatttcattc
    24181 tacctctagt ttatcatctg ttaaggacta gtggaaacgt tttgcctaac ttataagctg
    24241 atttttttag cagataagct tgcaagtaaa aaacaaacaa gctacaattg atttattgaa
    24301 attgttcctg tagctcagta ggatagagca atcgcctcct aagcgatagg tcgctggttc
    24361 aattccagtc aggaacgtat aaataacccc tgtatccttt gcggtacagg ggtttgtttt
    24421 gtatttgtgc caaaaatgtg ccaaactagt tatcattttt atataaacta tcgaacacag
    24481 tattgatttt ttctttatcc tgatcttcaa gctctcgaac aatatgaa
//
LOCUS       DAXWLH010000027        24476 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXWLH010000027 DAXWLH010000000
VERSION     DAXWLH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.027"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE36_000784"
     CDS             complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE36_000784"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFF8383202.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE36_000785"
     CDS             complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE36_000785"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFF8383203.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE36_000786"
     CDS             complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE36_000786"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFF8383204.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3545..4852)
                     /locus_tag="ACGE36_000787"
     CDS             complement(3545..4852)
                     /locus_tag="ACGE36_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFF8383205.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4855..5436)
                     /locus_tag="ACGE36_000788"
     CDS             complement(4855..5436)
                     /locus_tag="ACGE36_000788"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFF8383206.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5552..5649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6008..7300)
                     /locus_tag="ACGE36_000789"
     CDS             complement(6008..7300)
                     /locus_tag="ACGE36_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8383207.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7297..7920)
                     /locus_tag="ACGE36_000790"
     CDS             complement(7297..7920)
                     /locus_tag="ACGE36_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383208.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7923..8759)
                     /locus_tag="ACGE36_000791"
     CDS             complement(7923..8759)
                     /locus_tag="ACGE36_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383209.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8776..9663)
                     /locus_tag="ACGE36_000792"
     CDS             complement(8776..9663)
                     /locus_tag="ACGE36_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383210.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9679..10962)
                     /locus_tag="ACGE36_000793"
     CDS             complement(9679..10962)
                     /locus_tag="ACGE36_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8383211.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRIKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11645..12328)
                     /locus_tag="ACGE36_000794"
     CDS             complement(11645..12328)
                     /locus_tag="ACGE36_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8383212.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12346..12963)
                     /locus_tag="ACGE36_000795"
     CDS             complement(12346..12963)
                     /locus_tag="ACGE36_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8383213.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12973..13797)
                     /locus_tag="ACGE36_000796"
     CDS             complement(12973..13797)
                     /locus_tag="ACGE36_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFF8383214.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGTKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13790..14350)
                     /locus_tag="ACGE36_000797"
     CDS             complement(13790..14350)
                     /locus_tag="ACGE36_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383215.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14524..15480
                     /locus_tag="ACGE36_000798"
     CDS             14524..15480
                     /locus_tag="ACGE36_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8383216.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15785..16741)
                     /locus_tag="ACGE36_000799"
     CDS             complement(15785..16741)
                     /locus_tag="ACGE36_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8383217.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16845..17447
                     /locus_tag="ACGE36_000800"
     CDS             16845..17447
                     /locus_tag="ACGE36_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8383218.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17535..19034)
                     /locus_tag="ACGE36_000801"
     CDS             complement(17535..19034)
                     /locus_tag="ACGE36_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFF8383219.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19050..19574)
                     /locus_tag="ACGE36_000802"
     CDS             complement(19050..19574)
                     /locus_tag="ACGE36_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFF8383220.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19872..20621
                     /locus_tag="ACGE36_000803"
     CDS             19872..20621
                     /locus_tag="ACGE36_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8383221.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20612..21397
                     /locus_tag="ACGE36_000804"
     CDS             20612..21397
                     /locus_tag="ACGE36_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8383222.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21366..22082
                     /locus_tag="ACGE36_000805"
     CDS             21366..22082
                     /locus_tag="ACGE36_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8383223.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22184..23203)
                     /locus_tag="ACGE36_000806"
     CDS             complement(22184..23203)
                     /locus_tag="ACGE36_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFF8383224.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23222..24223)
                     /locus_tag="ACGE36_000807"
     CDS             complement(23222..24223)
                     /locus_tag="ACGE36_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFF8383225.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7258 a         5270 c         4269 g         7679 t
ORIGIN      
        1 tattcttcat cgatcattaa cagacaaaga actggcccgc caaggatatg aggacatctc
       61 cttgaagtac cagttcgtac actcaactta ttgaaccgcc gtatacgggt ccgtacgtac
      121 ggtggtgtga gaggggcgga aggtcagttg gctttcccct ctactcgatt atttttgcta
      181 ttctatttta attgaatttt gcaactcact cttctttatt ccaaacctac tcgtttgaat
      241 atcacgtcaa cgtttttcaa atgatgatga taatcaaagg catcgtctat ttcttcttta
      301 gataataccg atgtgatggt tggatcttct tctaacagcg ggcggaactg cacttggttg
      361 tcccaagcgt acgcagtttt tggctgaaca agatcataag cttcttctct tgtcattccc
      421 ttatcgatca atttcagcat aacgcgctgg ctataaatca aaccaaatgt tgcagacata
      481 ttccgcttca tgttttcagg aaagaccgtt agatttttaa cgatattgcc aaaacgattt
      541 aacatgtaat tcaataaaat cgtggtatca ggcaagatga ttcgttctgc tgaagaatga
      601 gaaatatctc tttcatgcca taatgtgaca ttttcatagg cagtgaccat gtgtccgcgc
      661 accactctgg ctagaccagc catattttct gaaccgatcg gattcctttt gtgcggcata
      721 gcagaagaac ctttttgacc tttcgcaaaa aattcttcta cttcacgtgt ttcggatttt
      781 tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg tagcaataag cgccatcgct
      841 gagaaatatt cagcatgtag atcccgaggg agaacttgag tagagatttc ttgtggacgg
      901 atccccaatt gtttacatac atactcttca ataaatggcg ggatatttgc gaacgttccc
      961 actgctcctg agatttttcc tgcttctact ccttttgccg catgttcaaa tcgttcgatg
     1021 ttccgtttca tttctgaata ccatgtggca agtttcaatc caaaagtagt tggttcagca
     1081 tggacaccgt gtgtccgacc catcatcact gtatatttgt gttcttttgc tttttcgcca
     1141 atgatttccg taaaacgttt caagtcttcc cgtaaaatat catttgcttg cttgatgaga
     1201 tagccataag cagtatctac gacatccgta cttgttagac cataatggac ccattttctt
     1261 tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa cgacatcatg gcgtgttaat
     1321 ttttcgattt ccaagatccg atcgatatca aatgatgcat gctcacgaat ttttttcaca
     1381 tcttcttgcg ggatttcacc aagttctgcc caagcttcat ctgctaaaat ctcgacttcg
     1441 agccacgcac ggtatttgtt ctcttccgtc cagatattcc ccatttcagg gcgtgtataa
     1501 cgttctaaca tgttttttct cctctaatcc caaatattcg tttgtttgat ttcttccagc
     1561 gtttgctgga tgtcttctgt caagatagtg atatgtccca tcttgcgacc tttttttgct
     1621 tgtttttttc catagaaatg gaattgccag tctggcttat tctcaatcaa gcgatagctt
     1681 tccagcagtt gttctcccaa aacattgacc attactgcat tgcttatcaa acgtactttt
     1741 tctcctaatg gccaaccaca aatacctcga atatgggcat cgaactgact catagaacaa
     1801 gcttcaatag aataatgtcc agaattgtga ggacgaggag ctaattcatt cacatatagc
     1861 ccgcctgttt ttgttaaaaa catttctacc cctaaaacac ctgaaaggct cactgcttct
     1921 gcaatcacac gggcaatacg ctcagcctcc tcgatcacat cctgatcaat cgcggctgga
     1981 gcaatcgttt catgaagaat attgttgtgg tggatgtttt ccacgaccgg aaaagtcgtg
     2041 aaatctcctt gaccatttcc tgccaccatg atcgaaatct ctttttcaaa cgggatccac
     2101 gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg caggagctag atccgcccga
     2161 cttttcaaaa catactgccc cttgccatca tagccgcctc gagtggtttt caaaacacaa
     2221 ggatagccga tcccgtcaat cgcatcttgg atatccgtcg gactgacgat cgttgcataa
     2281 ggagcaatca cgatgttatt cgtttccaaa aaagattttt ctaacaggcg gtcttgcgta
     2341 atcgcaagca agtctgtccc ttgaggaata aatgacatgg gtaaaatcgc atttaacgcc
     2401 tctacactga cgttttcgaa ttcataggta atgacatctg ttcttctggc aagctctttc
     2461 agcgcaaagg tgtcatcata gacactttca atgtgccaat ctgcaacttg tgcggctgga
     2521 caatcgctca caggatccag cacacctact ttaaagccca tttcttttgc actaatcgcc
     2581 atcatacgac ctaattgacc gccaccgacg atcccgatcg ttgaaccagg caacaaaggt
     2641 ttaaccaagt tgatcactac tttctataac agttttagcc aacattgaac gtctttcagc
     2701 caatttagcc tccaattcta aatcatacat ggaaagcatc tgtatcgcaa gtagtccagc
     2761 attaattgct cccgcttttc cgattgctgt agtagctaca ggtactcctc caggcatttg
     2821 gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt gattggaccg gtacaccgat
     2881 aaccggcaaa gttgtttttg atgcgaccat acccggcaga tgcgcggctc cccctgctcc
     2941 tgcaataatg attttgattc ctttttttcg agcgttttgt gcatattcaa acatcaaatc
     3001 tggggttcga tgagcagaaa cgaccttttt ttcataagac acaccgaatt catccaatat
     3061 ttgacatgct tctttcattg tttcccaatc tgaagtactc cccataatca cagagatttt
     3121 tgctgtcatt gtttttcctc ccagtttttc tacattcata ttttaacaat cacttttttt
     3181 gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt ttattgttcg tgtttattaa
     3241 agataaaaaa aaggaatgtg acaaaagtta ggtcacattc ctttttatgg ataaacggtg
     3301 ttctcctgcc aactcctcgg tcacaaggaa caatattcaa caaacatgag aagctttttt
     3361 ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct tccttcaacg
     3421 gtgttcaatc agctgaccct cggtattagc tcaaacaaac ggcaaaatac gaggaactat
     3481 ttttccgttt gtttctctaa caatcaggtc aaaacgctga tttcacaacc tttcttttgc
     3541 tctattactt cttgaaggct tcttccaata tttcttcatc ttttttcaat ccattaaaga
     3601 tcaaattcaa tatgatagcc gtaatgctac tcattacgat gccatttcca gtgaacattt
     3661 gcaatgtttg cggcatttga ctgaataatg tcggcgtaac attgaaaccc agcccaaatc
     3721 caacagaaac agcaacaatc aatagatttt tatcattgct gaaatcaact tccaacagca
     3781 tacggattcc ttggactgcc accatcccga acatgactaa cattcctcca ccaagtaccg
     3841 gctcagggat gatttgtgct aacgcaccga ttttagggaa taatcctaaa acaatcaaga
     3901 agaatgcaga gaaatagatg ggttttcttg ttttgattcc cgaaagttga accaatccga
     3961 cattttgaga aaagccagta taagggaatg tattgaaaat accgcctaag ataacggcaa
     4021 gtccttctgc acgatatccc tttttcaatt cttcttctcc aacatttttc ccagtaatgt
     4081 caccaagtgc aaaatataca cctgttgatt ctaccatgct gaccatggaa atgatgatca
     4141 ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa ataaaagggt tgtggaacat
     4201 ggaacaacgg cgcatctcca atcgcgctca gatcaatcat tcccatgaaa gcagctaaga
     4261 tgcttcctcc gactaaacca atcaataccg cgattgaacg aatgaaccct tttgccagca
     4321 tttgcacgcc tacaattagg agaatcgtca caaatgcaag tagcagattt gttttgtcac
     4381 caaaatcagc tgctgttgca ttacctccac ccattttctc gattgctaca ggaatcaaag
     4441 tcaaaccaat aacggtgata accgttcctg ttactatagg agggaatagt tttttaattt
     4501 tagaaaagac accagaaact aacacaacaa agatcccaga agcgataata gatccgtaga
     4561 tagctcctac tccttcattg gttccgatca agatcagtgg agctactgct tggatcgcac
     4621 atcctaaaac gacaggcaag ccaattccga aaaatcggtt aactgtcaat tgcaataatg
     4681 tcgctaatcc acacataaag atatcaattg aaatcaaata agtcatctgt tcttgattaa
     4741 agcctagtcc tgtaccgatc aaaagaggca ccgctactgc tcctgcatac atcgctagca
     4801 gatgctgcag ccctaaaacc gcagcttttc cattttgtgt ttctttttcc acgattatgc
     4861 gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa gcaatacgcg caagagaaac
     4921 gactctcaat cccatttctt caagtaactg acgtccatct tggaaagact tttcgatgac
     4981 gatcccgatt ccttctactt gcgctccagc ttgctggcat aattcgatca atccttttgc
     5041 cgcttgtccg tttgccaaga aatcgtcgat aatcaatact ttgtcttctg aagataaaaa
     5101 tttacgagaa atagaaatcg tacttgtcac ttgtttcgta aaagaataaa cagaagctgt
     5161 caataattct tcatccatcg tcaaactttt tgcttttcta gcgaagatca ttggaacatc
     5221 taattctctt gcagcaaata aagctggtgc gatcccagaa gcttcaatgg tcacaacttt
     5281 cgtgattcct tgttctttaa atacttctgc aaaccgtttt cccatttgtt ccatcaatac
     5341 agggtctact tggtgagtga cgaagctgtc gacttttaat acgccctcgc ctaatatacg
     5401 tccgtctttt tgaatacgtt caactaattc tttcacttta tttgctcctt tatcatttat
     5461 taataccgaa ataaccgctt ttgtgcaaaa acctactatg tttctgttaa agaaaaacac
     5521 cctccacttt atcataagaa aagaagggtg aaatagccaa tcttttactt atagtccagc
     5581 atttacggta ctggctagaa actgtcatcc atattatgac gatatataag taggtatgta
     5641 aaactaataa tagtgtaaat tcattttctt gcaactgttt tgcacgtgaa ggcagtttgc
     5701 catatttgaa taaaaaatgt tgtttcaaat attgaaaaca gtctaacact actttctgaa
     5761 actgacaacg tggtttactt atttttttgt aaaatctaat gatttttaat gcattattcc
     5821 tttttgttcg ggtttaaata gttattttta ttttctccta tctaaaaaag cagcccacat
     5881 aaaaagaagg catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa
     5941 aagacagcct atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa
     6001 tctattctta ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa
     6061 tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg
     6121 cccgattcca cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat
     6181 ctcctccgcc atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca
     6241 tcggatagac atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg
     6301 cattgatgta agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag
     6361 aaacattgaa tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag
     6421 tttcatattt gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac
     6481 tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct
     6541 cattggctga caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc
     6601 gactgtaaaa tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc
     6661 caccgaatgc aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat
     6721 gatgggtaga tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc
     6781 tgaaataatc tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat
     6841 caccaatcac atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat
     6901 aattaaaagt gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag
     6961 ctttttcctc atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg
     7021 cacgaatgat ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc
     7081 gtacgtcatc cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc
     7141 catacttgtt cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa
     7201 aaccgactag ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg
     7261 ctctcccacc aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc
     7321 ttttaagcaa aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa
     7381 atcatgctgt atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt
     7441 gacgtttttt gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt
     7501 atcttctttg cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag
     7561 acacaataga aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc
     7621 aactgcaaaa ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat
     7681 ttttcctttc ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga
     7741 aggatacatc atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa
     7801 agtgaacaaa catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa
     7861 aatccgacag acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat
     7921 attcagccct tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag
     7981 aaaatcagaa aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc
     8041 gtactagatc cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc
     8101 ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg
     8161 atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac
     8221 ttgctgcatc cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat
     8281 tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca
     8341 tacgtcccaa ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt
     8401 gcaggaatca tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg
     8461 attctcgata atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact
     8521 gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc
     8581 catccttttg cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc
     8641 ataatctctt gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg
     8701 actagaccta atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt
     8761 tcttcctcct ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt
     8821 gacagctccc ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt
     8881 aaaagaaaaa gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc
     8941 accttgggta atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac
     9001 caagttatac aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca
     9061 tttgttcgct ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc
     9121 tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat
     9181 catgaccagt ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa
     9241 aatattcact aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact
     9301 gccaccgatc aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt
     9361 cctcgttaaa agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa
     9421 tacatagaga aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat
     9481 ataattatcc aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata
     9541 ataaagtgac aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga
     9601 agcaaatacg taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt
     9661 catgccttcc tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc
     9721 tgcttcttct gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc
     9781 ttgaatttcc ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga
     9841 aatcatttct aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg
     9901 ttctcgaata ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc
     9961 ttcactgttt tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt
    10021 tgccatactc agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttat
    10081 gcgaggtgga ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt
    10141 caactggtta cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga
    10201 cagatccgtc gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag
    10261 atttttacgc agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt
    10321 agatagatct tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat
    10381 acagccagct ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat
    10441 ttgatagctg atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt
    10501 taattgttca aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag
    10561 tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat
    10621 atcaattatc ccttgctcca catattcatc taatggttca atctgattga tgtattgagg
    10681 aaattgattg ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa
    10741 ttttgtgtaa tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt
    10801 ttcttcataa agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca
    10861 agccatgttc agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa
    10921 cataacactt aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc
    10981 cattgttatt tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt
    11041 tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata
    11101 ctttcaagaa aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt
    11161 caatgatggg aggataatta cgcaaagaaa aaaggatata agacaactgc ccaatatcct
    11221 tgacatagct aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata
    11281 actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat
    11341 ggcaacttga ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta
    11401 aattgtcggg tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat
    11461 aaatcaagac atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat
    11521 gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt
    11581 actggagtac aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca
    11641 cctgttattc atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa
    11701 gctctccgct acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag
    11761 caatactttc ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac
    11821 gaaaatggcg tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac
    11881 cagctgctaa cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa
    11941 tatcaactac tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga
    12001 cccctaacga accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg
    12061 tcaaaccacc acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag
    12121 taatcgctgg atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac
    12181 gtatgatcgc aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga
    12241 tcccgcttgc ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca
    12301 tgatttctgg cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata
    12361 ctgattcaat tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt
    12421 cgcataacct gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac
    12481 tttttcatac agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc
    12541 catggactgt tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc
    12601 gttaacaaat tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc
    12661 gaaaagatta ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc
    12721 ttgtcctaaa atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg
    12781 aacaagccgt tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt
    12841 aaaaggagaa gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc
    12901 ggcaaccact gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc
    12961 catgatcaat tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg
    13021 tttggtgata tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt
    13081 gatcttagtt atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc
    13141 attatctgcg atccatttag ttccaggctg atctccgtaa cttgcatcca atacagtaga
    13201 aggataactg ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc
    13261 gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac
    13321 accttgacga ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac
    13381 agaccggtaa gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa
    13441 tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa
    13501 ttgagtagac tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt
    13561 ccaatcgtct gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc
    13621 ctttaccttg ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct
    13681 tgttcgttcg actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac
    13741 atagcccatt gctgcgatca taatcacaac tgctccaaat cctaaaaatt tattcacttg
    13801 aaaaatcctt tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca
    13861 gattttcatt cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat
    13921 ctgctggcag acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta
    13981 ctttcaagtc aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt
    14041 ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg
    14101 caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta
    14161 gcatccattc ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat
    14221 ttttctcgcc atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta
    14281 aaaaattttt ttcagtttca aactgttcta ataatacact cataataaga tcaatatata
    14341 cttgatgcac gtttatcccc tccttttaac tattctactt aagttttgat caaaaagcac
    14401 ctcttaaatc gctgctttca ttttttttaa tgtttcaagt cctctcctct ttattcttcg
    14461 tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa ggagcgtttt
    14521 cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc
    14581 gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac ttcagaaaaa
    14641 gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta tcaagaaatg
    14701 ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca gcatatcgac
    14761 catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc catcacgatg
    14821 aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt gatcttagca
    14881 gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat aatggataca
    14941 ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa agaaccagac
    15001 cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga tatcggtacg
    15061 tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt tgcctctaca
    15121 atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg taacaaagaa
    15181 aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt
    15241 gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg tgcggagatc
    15301 ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga agcaatgaat
    15361 tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg ctctctcttc
    15421 ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa aaatatgtaa
    15481 ttttaaaagg ttgtgaaaaa gctgttctgc atcaattaat aagaacagcc aaacaaaaat
    15541 agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct tttgaacacc
    15601 gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag caatttcaaa
    15661 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg acaggagaac
    15721 accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc tgaattcaca
    15781 acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata aaacttttaa
    15841 aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga ctagtcggca
    15901 gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt tgtctgccat
    15961 catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata tgttttccat
    16021 aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc acattggcct
    16081 gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc cagtagtttt
    16141 cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt
    16201 tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt ctcaaaatat
    16261 ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta cgatctggca
    16321 tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc gcttcattgc
    16381 cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa accgaattta
    16441 ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa
    16501 gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct gttgccgtag
    16561 ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc
    16621 ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat aagctacccg
    16681 ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt cctatacgca
    16741 tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag ctatcagaca
    16801 aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt
    16861 ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt atcttgtttt
    16921 ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc
    16981 gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag
    17041 gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg
    17101 tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta
    17161 acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc
    17221 ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat
    17281 tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac
    17341 tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag
    17401 agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa
    17461 taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg
    17521 tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt tggcgcttca
    17581 tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag
    17641 tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa
    17701 tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag
    17761 attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg
    17821 ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt
    17881 tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct
    17941 aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg
    18001 gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg
    18061 gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt
    18121 tcttccagtt cgatttgttt ttgacgttct acgattttaa tctgcatttc ctgttcgatt
    18181 acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca
    18241 acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg
    18301 atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg
    18361 atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg
    18421 attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg
    18481 aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt
    18541 tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag
    18601 cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct
    18661 aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa
    18721 gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt ggagacgtcc
    18781 aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg
    18841 ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt
    18901 cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct
    18961 acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca
    19021 actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg
    19081 acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc
    19141 tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac
    19201 tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc
    19261 agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag
    19321 aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa
    19381 gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa agttaaccaa
    19441 gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc
    19501 aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg
    19561 attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa
    19621 aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat
    19681 cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga
    19741 ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc
    19801 ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt
    19861 ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg
    19921 ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca catgcgattc
    19981 aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt gtcatggacc
    20041 ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc
    20101 atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt
    20161 tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt attggtgcag
    20221 tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga caaaaaatga
    20281 ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt tttcttgccc
    20341 aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg
    20401 aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa
    20461 ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg
    20521 atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta aagagacgat
    20581 tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag aaactatttc
    20641 tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa
    20701 tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat atataaactc
    20761 catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg gtttgattga
    20821 tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa attataaaaa
    20881 agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt ttttattagg
    20941 caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg attcttttat
    21001 ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag ggaacttact
    21061 tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg tccggattca
    21121 tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg acggtgtcgg
    21181 gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga agggaatcaa
    21241 tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg gatttttaga
    21301 attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa aaaaggaaga
    21361 tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca gaattgaatc
    21421 gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga aatgtagaag
    21481 aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat gtatacatgc
    21541 ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat gatatcccag
    21601 tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc cgctacggtg
    21661 ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt
    21721 atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc
    21781 aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat ttgcctaaag
    21841 gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa gatactattt
    21901 tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc aagaaatatt
    21961 tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg gaaatcggcc
    22021 ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca gattactgtt
    22081 gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca actgtttact
    22141 tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt aattgacggg
    22201 ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca cgaaagttct
    22261 cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct acatgcgggg
    22321 ttacaagaac tctagggtac atttctacta attgctggat agcagggtct ggaatcgtct
    22381 cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa
    22441 aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct tgaagctctc
    22501 cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat ccttcattga
    22561 tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata tcgcttttgg
    22621 ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct gtctgatgaa
    22681 tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc gtcaacccta
    22741 tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta aacatttgct
    22801 catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa
    22861 gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac gatggtacac
    22921 gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg actcccacag
    22981 tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg gtctcatctg
    23041 ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg atattgtcat
    23101 gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat ttttcaaaaa
    23161 aatctatctc gttcggacga acaccgtaac atgcgattct catactttct tcctccattt
    23221 cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat acaggtgtca
    23281 gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt gcctccctat
    23341 caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc atgataacga
    23401 ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa actgctagaa
    23461 tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac catgaagttt
    23521 tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt gctagccacg
    23581 ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac ggtgcagtct
    23641 ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta actgacactt
    23701 gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg ataatgattg
    23761 gattgacgaa catcgctttg atatttttag cttccatctt caagccactc atttttatgt
    23821 atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa tacatcacgc
    23881 cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga
    23941 tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa aaaggttttg
    24001 aaactagaat aagaataata taaataacta atccccaaat caagacattg attccttgca
    24061 tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct ggcaaagcca
    24121 ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc cccttttttc
    24181 tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg acaatcgaca
    24241 tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat ctttctcctc ctaattatgt
    24301 tcatttattc acaataaaaa aagcttttat tttgctaata cgtattaaca tttttaattt
    24361 tatctttaca ttgttactta ttgaacaata ggaaattaaa gaaacttttg aaacttatgt
    24421 aaagtgattt ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga agttta
//
LOCUS       DAXWLH010000028        24329 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXWLH010000028 DAXWLH010000000
VERSION     DAXWLH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.028"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(280..495)
                     /locus_tag="ACGE36_000808"
     CDS             complement(280..495)
                     /locus_tag="ACGE36_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383226.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            complement(547..1461)
                     /locus_tag="ACGE36_000809"
     CDS             complement(547..1461)
                     /locus_tag="ACGE36_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383227.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(1461..2360)
                     /locus_tag="ACGE36_000810"
     CDS             complement(1461..2360)
                     /locus_tag="ACGE36_000810"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFF8383228.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(2360..2947)
                     /locus_tag="ACGE36_000811"
     CDS             complement(2360..2947)
                     /locus_tag="ACGE36_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383229.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(3111..3302)
                     /locus_tag="ACGE36_000812"
     CDS             complement(3111..3302)
                     /locus_tag="ACGE36_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383230.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(3375..4277)
                     /locus_tag="ACGE36_000813"
     CDS             complement(3375..4277)
                     /locus_tag="ACGE36_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383231.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(4294..4770)
                     /locus_tag="ACGE36_000814"
     CDS             complement(4294..4770)
                     /locus_tag="ACGE36_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383232.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(4786..5385)
                     /locus_tag="ACGE36_000815"
     CDS             complement(4786..5385)
                     /locus_tag="ACGE36_000815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8383233.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(5944..6423)
                     /locus_tag="ACGE36_000816"
     CDS             complement(5944..6423)
                     /locus_tag="ACGE36_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383234.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            complement(6442..7071)
                     /locus_tag="ACGE36_000817"
     CDS             complement(6442..7071)
                     /locus_tag="ACGE36_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383235.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(7068..8444)
                     /locus_tag="ACGE36_000818"
     CDS             complement(7068..8444)
                     /locus_tag="ACGE36_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8383236.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(8524..9786)
                     /locus_tag="ACGE36_000819"
     CDS             complement(8524..9786)
                     /locus_tag="ACGE36_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383237.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(9799..10146)
                     /locus_tag="ACGE36_000820"
     CDS             complement(9799..10146)
                     /locus_tag="ACGE36_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383238.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(10166..11035)
                     /locus_tag="ACGE36_000821"
     CDS             complement(10166..11035)
                     /locus_tag="ACGE36_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8383239.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            11338..12783
                     /locus_tag="ACGE36_000822"
     CDS             11338..12783
                     /locus_tag="ACGE36_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFF8383240.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(12800..13588)
                     /locus_tag="ACGE36_000823"
     CDS             complement(12800..13588)
                     /locus_tag="ACGE36_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383241.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(13694..14494)
                     /locus_tag="ACGE36_000824"
     CDS             complement(13694..14494)
                     /locus_tag="ACGE36_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383242.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(14833..15942)
                     /locus_tag="ACGE36_000825"
     CDS             complement(14833..15942)
                     /locus_tag="ACGE36_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8383243.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            complement(15932..16789)
                     /locus_tag="ACGE36_000826"
     CDS             complement(15932..16789)
                     /locus_tag="ACGE36_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8383244.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(16864..18699)
                     /locus_tag="ACGE36_000827"
     CDS             complement(16864..18699)
                     /locus_tag="ACGE36_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8383245.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(18748..21138)
                     /locus_tag="ACGE36_000828"
     CDS             complement(18748..21138)
                     /locus_tag="ACGE36_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8383246.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(21406..22653)
                     /locus_tag="ACGE36_000829"
     CDS             complement(21406..22653)
                     /locus_tag="ACGE36_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8383247.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            23045..23461
                     /locus_tag="ACGE36_000830"
     CDS             23045..23461
                     /locus_tag="ACGE36_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383248.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(23478..24215)
                     /locus_tag="ACGE36_000831"
     CDS             complement(23478..24215)
                     /locus_tag="ACGE36_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383249.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
BASE COUNT         6898 a         4839 c         3473 g         9119 t
ORIGIN      
        1 ccatctttaa agaaatacga tcaaaaaaca taattcccat attatttgct cgttcaacaa
       61 tttgaaatat atctattagt tcttcttttt tcataactgc tcttccttta acttgttaat
      121 ctaacttgct tacaaatata tcctaccaca tttgtgtact atctgtcaat atttttttgt
      181 gtataaatgt gtattataaa acaaataatt ttacacaaac aaaaaagagc ctaactgctc
      241 ttagcaataa gctctcatca ctgctcctcg tgaatttagt cagtacaatg tatttgcctt
      301 ttctttgata aaatccttat acgaaaaatt gttcaaccaa aattgtttta gcactaccga
      361 atttttgttt ccatcattca actccaattg accaaatctg ctgatcatat catcaatttc
      421 atcaattatc tcttgtgcca tcatgccgtc ttctagacgt tctaatacgt acaattcaaa
      481 aacatccctt ttcattcaaa atgccccttt caggttacct tttgccattt tatgggctaa
      541 ctccgcctac tcagcttttt ttatatacct actgatattt gctttcgtat ttcttggcat
      601 accacctgct tgcaggtaat gttcacgata ataagcatct agctctgaaa cagctttttc
      661 taatctattc gttgcattca acactgagcg gtattcacgt ttttccctta atacacgtct
      721 taaatggttc atcgctgata tataaggctt atagtattcc aatacagtat atcctctaga
      781 aatataggca attggatttc tagcttcatg gatcaattct aaaatatctg ttggatcagg
      841 cttatccggc atctcttctt cgatttccat aatactgatc aagtagtcca atccggaatc
      901 atcgactcca taactttcac accattcttt taggttgttc aagtcatgaa taacatcttc
      961 aggatcaaac tctctaaaag cagtgatccc ttggctataa tattttatat attccattgc
     1021 cgattcaaaa tcctctggcg gtatgaaagc ttcaatttct tcttcatcaa tcgctttcag
     1081 cactattccg atacctgagt ttgaaggata ttttctcaat ccttcttcaa ttaaatcctc
     1141 atctattcgt cctttttgaa acatcaccaa taattgttct aacagctgat ctaaaaccct
     1201 gcgctcttca ttcagttcca ttgcattttt aacttctttt tcaccgagtt caaagtaagc
     1261 tttgatcaac ttttcttgtt cttttcgttc aactaatttc ttcttcaagt cattcatatt
     1321 gagaatgaaa tccatgtgat tactcttttg ctgattgaca aaatcatatc tttgttccaa
     1381 aacatcccga tattttttta gttcttcttg aattatttca aagctatcaa gtacatgaag
     1441 aattttttca ttattattca tcagcccatc tcctgtagtc tcacgtttgt tttcgacttt
     1501 tcaaaagtat tgtatttaaa agcaattaac cctacaaccc tgctatcttt gatgcctatt
     1561 tccttgttaa aaattccgtc caatgttcct ttgactaaat tatcaatatc cggctgtgaa
     1621 gtacgcatca gtgaatcaag ctttttctta tcttttctca cttggtaaaa ataatctatc
     1681 tgagctataa cccccatagg agcattcatt acctttttca gttgatcatt taccaaatct
     1741 ttctctttaa gcgtattctt gatattttcc aaatagtcgt tgtactcttt tggatagtaa
     1801 gtgataatcc catttcctct aaatttagac tttggtcttg gcgttgctac tggtttaaaa
     1861 tcaaatgtag cttcccatat atctccgttc aatccttcag aagtatccca ctcaaattct
     1921 gaatatcgtt tgatcctcac aactgttttt ccttcgttcg ccacattata tttaaaagca
     1981 attaagcctg tgatatggct atctcgtata gctgagatgt taaaaatacc atccatagca
     2041 ctttttacta aattatctaa atctggcgct gttgtcttca taatgttata gactttttta
     2101 tagctttttg gtatctggta gtaaaagtcc acttccataa ttgcgcccat aggatcattt
     2161 acaatcgagt aaaattcatc atctaataaa tcttgatctt cgataaagct ctttattttt
     2221 ttcaaataat caagatactt tttatcatag taggttgaaa tccttttctc tgctacgtca
     2281 ttagtttcat cagcagcatt ccttcttgca cttggtcttg gtgcagatac aggttggaac
     2341 ggtaaagtta cagtccacat tagtcttttt tgatctcttt caaatagatt tttttaaagg
     2401 tatcaaggct taaaggagct accttttgca tcttccggat tttatttact tgttccaatt
     2461 gtttcaatag accatcttct tttctaaggt actcaggatt tgttatttgt ttattccgat
     2521 actcgattaa tgattgaagc tctcttactg ttaaaacatc ctcaattttt gcttctttac
     2581 tcgttatctt ttttagatac cctaatactg ttggataaga tttgccgatc acttctgcga
     2641 tttctttttt attgtactca ccaaattttc caagcaacca gtcattcatg atcttacttg
     2701 atacttttgg gtatacaaga attgcttttt caatggaatg actgccatta tttctgatca
     2761 gttgactatc tttacactca actgtaacgc catcacctaa atcaacaaca tattgccaaa
     2821 cgcctcttcg attcttatta agagaaataa tcgttccctc tttatctcta taatcctttg
     2881 ataattcact tactgttgta actctttccc caatgctaaa atgtctaata tcattattgt
     2941 tttccactta ttatccctcc aatattgctg gttatctctt cctctgacaa ggaactatat
     3001 atcatataaa gaattttaac attattcgga atgagtgtaa agaaacttta tataaaaaga
     3061 gtgatttagt ctaagaccaa atcactcttt cttttttatt aattatattg ttatctgata
     3121 gacctattag taaatgcacg tcttattgtt tgatcattct cgatcaattc ttctaacaac
     3181 tctgaaactg ttactttctt acctcttgct ctttcttctt ctttagctcg cttttctaaa
     3241 tatttaacag aagctatttt taaagtcaca tactttctaa ctttttcttt atttactacc
     3301 atcagctatc atctccaagg tgtcacacct atatattatt ggtgtgacac catttgttta
     3361 ttataacttt ctggctattt agccaattta atcgttaatg cttcgtttgc tgtagaatct
     3421 ccaaaaactt taccgtcttc ataaactaca tgaactggca aatctgcacc gtctgaatct
     3481 ggtttagcag taccacgata caaaccaatt actctttttc ctagttctct tgcaaccgta
     3541 acttttttat cctgatccat agattcaact gaattgaata aggtaacatt aatcatttgc
     3601 ttttcagtat ccaatttaac agttttgata gctttgaaga cttcagtgtt aaaccgatca
     3661 gccatagaac cagaaacaga ttcaaacgat ttgacttttt caggatcaaa atcaatttta
     3721 tctggtagtt ccgcttcgac agtagaactt gattcagttg tactttcttc tgtagctact
     3781 tcttttttcg ctgattcgat aatctttttc gttgatgttt tatttatttt tacatcttta
     3841 tagtattctt ctccatcaac gttaaaaacc aatgtcagct tgtgtacagg gctattctct
     3901 ttaactgtat aaaacacttc aaattgtgat tgagacggct taatagtagc gagaacgtcg
     3961 tctgatgctt tcttgccatc ttccttcact gtcagcgaaa catttttata actcgttaca
     4021 ttttcaaatg ttccatctat tactaccctt cccaaagaat ctggagcata actattatta
     4081 actcccgata taccaaattg tttatcgtta cttccaaaaa tcttagtggc attaaacaca
     4141 ataaaggtta caatcacaat tccaatgcct atgttacgca taatcggatt atttgtttga
     4201 aattgaccaa taattatagc gattgcccca aaaaaagcaa gaattaaaca taaataacct
     4261 aacagggtta atagcatgat tcatcacaac tttctattct ttatttttct ttttggtttt
     4321 cgtttgttca aactcatgct catttagata agtaatcaat aaatctcgca tggatttgct
     4381 atccttactt agcgtatcaa acgcttcttg taagtcagca tccttctcta cagcattaaa
     4441 ccaatgcttc gttccaaatt caaaaagatt actaatcact cttaacggtt cttgatcagt
     4501 attcgtatta agccattcaa gtatccgttg ttgagctaat cctaatttac tttcgtcttc
     4561 gtgatttgta cgtgcttcat agattttagt gtgtatatca tcaaaatagt ggattcgttc
     4621 tcttagcgct tcacgttgtt cgtacatagc ccttttattt ctaacttctt cgtctattgt
     4681 ttcaatcttt tttcttaaca gtatttgaag tgataacatt tctgagacag acaaaaacac
     4741 tggaattttc ttttcttttc tttttggcat ttttagcact cccttttaat tcttttgttt
     4801 gatcaatgaa acgttcttta gaataaatcc caagatcatt ccacagacaa atccgattac
     4861 atgtgacaag acatttgcct cgcctgattc aaaagtagat accaataaaa tgccgattat
     4921 caataataca aaaacactta gtaataaatg tttcttgcta tcaaaacgga tgtatgcaat
     4981 cgcaaataaa gcaatccatc caaaaatacc tccactaagc cccatactta cgatgttgct
     5041 gtgtagcaga aaaggaacta agcttcctag aaatcctgat atgattaaaa cgatcaataa
     5101 tgttaaccac tcatactgat cattcagtgc ttcttcaaga actactaaga acaataaatt
     5161 agaaattaaa tggtacaagt taatatgaga aacgatagaa atgaatatat tcgggaacaa
     5221 gtgtccttca tacaaaccac ctaaagcaac catttccttt gcactcaatg tatttccaat
     5281 tatgcttttt ccaatctgca aaaaatcaaa caggaaaatc aatacacaaa caaaaactac
     5341 agtcgctact aggtatcgac tactattttt gtttttttct ctcattcgct acttcctttg
     5401 ctttagtaac tgccgattct ccatattctc taaaaagagt cgatgttagc tttcctctcg
     5461 gaatattgta tttttttaaa atttcagttg ttgaaagatt tttttcaaag tcttcaataa
     5521 tttgtttatg aagatttatt ttagccaaat cattggttgt caaaacaggt tctttagctt
     5581 gaatatcgcc ttgacgaatt aacctttgaa ttttattttt gctttttaaa aaaggatatt
     5641 tttcaattaa agggtcaatt gcattccctt catcaatcca cttacctatt tcttgataaa
     5701 ctctcttttg aaattttttc ctcagctcaa tcgggttcgg aaaaacattg tgcttaaaat
     5761 cgtgtgatac taggctcttt gatttgccta attcatcagc gacttcttgc attgtaattt
     5821 tgttttcgat atagcattgg acaagagcat tatatttttt agggttatta ttatttattg
     5881 tcatgttctt acctacgttc attgctaatt ttttggtatt tttctgtttg tgtattgcga
     5941 tttttaaaag gcatttccta atacgttaaa ttttgataat cggttcaatt ttactgcttt
     6001 atcaatttct gcctgtgacc aaggttctat taaacgcatt tggtacgtca ctagcagatc
     6061 gacatgttca tattcaaact tttctattcc gtaaatctga taaaatattt tttcttcgtt
     6121 gttactataa ggaatcactg ttccgatctt aaatatgttt cgtgtgttgt ttacaccaat
     6181 ttcatcagta atcttgtgtc tatattctaa taacacgtat ttcttatacc tgcttgtcag
     6241 atcaggtgaa tagttaacag attgacaaac caagtcactg gttatacgtg gatcacctat
     6301 tctgttaatt ctcgtgttat agattccgat aataatatag tttttatctt tatattctat
     6361 agtttctccc acttctaact tttgattaaa gcctgttcta tttgcttcaa aaagttctag
     6421 caatcttttt cacctctact gctactgcca aacaaattta gctttatcat gccatgtttc
     6481 agacattact tttttaccat tttcgtcaaa tatcaccgct tcccactttg ctgttccatc
     6541 cggctgttta caaatatctg caaaccatag gatcgttttt cgatcgttga atttccttac
     6601 tttcttctca aatttattga tctgaactct attttgattg ctgttccaac ctactaatag
     6661 gcggttatct gattctgtac catgaaaacc aatgggaacg atgtgggttc tatcaaatgc
     6721 ttcttttcct tctggataga gcattggtct aagtttccta cgtttcaagt catctcttgc
     6781 ttgataccgc catgccgatt ttggattgaa tttaatagct atttgatgct tgcttttgtc
     6841 atggtaaaaa tatgcgacat cggcttcagc tccaagcaac ttttctcttg cactatttga
     6901 ttcaaacggt ttattcactt ctaaaacctt cacccctaaa atgccatgat caagcatctg
     6961 tttaggagtt agtaccgctt ttttatctct tttcttcgct cgtttcacaa ctacttcatc
     7021 tgtatattta tatggagtaa gccatcttaa aaaagaactg acgatgatca tttatgcacc
     7081 tgtttttttg cttttaacat tttttgcatg ataggtgaag tacttccatt tatttgtttc
     7141 ttttcagatt tatcagttgt gatcactacg ttttctgagc cttttggatc attagcttta
     7201 ggtttggttt tagatgcaat cggctgttta ttattcgtgt tgtttgaggt ttgcggttca
     7261 ggatcagctt ttcctaatgc ccttaaaact ttttctagtt tttctgcttg cagtttttct
     7321 agtgttttag ctccctgctc gactttttct tctttgttct tttcaagctt aatagcaaac
     7381 gcactcttct tttcaattga tggctcttta gatgatggat ttgctaaagg tgtgttcaaa
     7441 tttcttttag cagattttct tacaggagct ttaccaattc caccaaaatc ctcgtcttca
     7501 ccgccctcac cacgagtttc agctacgatt gctcggtaat caacatattc tggttctggc
     7561 atagtatttc taaggtgatt gaagattgtt tcaatttctt tgttactgat gtaagctccc
     7621 tgcgctctaa ccatacttcc atcaacaagc ttaataagca tatctccctt tggacttaga
     7681 ctttctccac cgccttcatc taatacaatc ctactttcta aatcacttgc taccataagg
     7741 caaacacgtg acggaaaatt ggctttgata ataccagtga caactgttga tcgaggagat
     7801 tgtgttgcta agataacatg aatacctgct gcccttgcca tttgtcctaa ttgaacaatc
     7861 aaaccttcta cttctttatg tgttccaacc aactgtgaat attcatcaac taataagatc
     7921 acatagggaa atctatctaa tccatttttt tcggcatatt cgttatatag cgtgatgttt
     7981 tgtaaccctg ctttttccat cagtttgtaa cgttcatgca tcaagatcac aagatattca
     8041 aaaaaatcat aagcttcatc catatctgta attggatcag ccaacatgaa cggcaatttt
     8101 ttgtatcttc caaattcaac cctttttggg tctacaattc ctatttttaa ttcatcaggc
     8161 gttgagtgac acattactgt aacaagaatt tcattcagta atactgattt ccctgatcct
     8221 gtcgttcctg caaccaacat gtgtggcatt tcgctgatgc aaaaatgagc aattgtgcca
     8281 tcaggcttaa ttccaaaaat agcatctgtt gggtctttag cctttgtgtt caacatattg
     8341 acactgaatt ttccattaag ctttatatta ggttctttat ctagcaaaat agattcagct
     8401 ttttcggctc tctttttgac tttctctaat aatttcatag ccatttctat tcctcacttt
     8461 tctcggtact tttctgtttg tgaactggta gtttgtattt tctgtttcta tttgtgatgt
     8521 cttctaaaac acgctattga tcattgatgg tacatcaatt ggaatacgat cattttctcc
     8581 taatggtaaa gtaatttgaa tacgccctgc gttgatccgc acgattacac ctgatatatg
     8641 caagtaagtt tcaatttctt ctttcattga gcttagttga atttttctgg tacgagataa
     8701 agtaaacaaa tattcaatag atgtattccc aacgtgcata tctgttagac ttgcaggtac
     8761 gtcagcagaa gatagataaa gttctaaaga ttgcttttga gtttcggcat attttctagc
     8821 tttttcggtt tggttttgga tatttttgga attatcggca aagagattga ttggaaaaga
     8881 aaattgtgaa tttgaaactg cgattgaaga tgttgctctt tccgtttctt tgtttttggt
     8941 acttactctt tccttacgtt ctctttcaat atttactgat gctcggaaaa tgaaataatc
     9001 gcctgaaact tcatcttctt tttcaccaaa tttggctcgt ccgcttgttg ctcttgtcag
     9061 gatcgaccct aaatcttgaa tttgtcgtaa aactgtttct ctaactttct tcccttttgg
     9121 ttgcgggatt tttacgatat attctcgaat atcttcattg acacttagat cacgtcttgt
     9181 atgaacagaa attttgatat ttttaagcgt tgatagcaca tcggattcta attgcgtttt
     9241 gtttcgtttt ctttttttgt tgttcgtttc atctcctata atcttgtttg gatcatactt
     9301 cacatccata gcttctagag cttctctttt caaccttcta ctttccatat catcaaaaaa
     9361 tggtctttgt tctccatagt gatgcctgaa catcaaagta accaaatatc caacaaagaa
     9421 taccgttcct gcttctaata ctaaaataga gatgtctacc aagtgtagct ttgcaaaaaa
     9481 gctttgtaca ttatatgaat aacttaaata attgggaaaa aacgcattga gtaactcttt
     9541 aaacatctct tttaatccta aatcatctgt tccgttaaca tcatttaacg agacaagaat
     9601 acttactaag gtatctacaa gcagttgtac aacctttgtt acccaatgca aaatatatat
     9661 gccaattgca ccaactacca atgcacgtcc aaccagatat actgatttag aaaaactggc
     9721 tacaggataa tatttttctc catcgcttcg cttatattcc tttaattcaa tggatttttc
     9781 gctcatctga atatctcctt attttctttc tgattttggt tcatcgtcac ttatgctgtc
     9841 ctcacacatc tgcgtatctt taggaatgat tactctgatt tcgtctaaca tttctctctc
     9901 aatgatcatc gccccttttg tactaacagt tttctctgat aattcaacac atttccctat
     9961 ttcagaatct ggatcaaaaa tagcctgttc tacgtcaaaa tgattcttag ctacatacac
    10021 gatactcatt gtttctaact ctctagcaac tgaatattta cccttcttca gaagatagta
    10081 gccatttcct ttttttattc gatttgcaac tgatttatct aatccattga tgcttattgt
    10141 ttccaattct tcaccttctc aatttctaat ctcttaccca agtaccattt tgatagttga
    10201 tgttgattcc tttttgattg ttaaatacat aaacattaag gtttaccgtt tttccattat
    10261 cgttaactga gaaagcttcg acatgagaac cacgaggaac taattcattc cctttataaa
    10321 ttggatcaac tttatacaat actttttcat tcttattttt atagattgca tcacgtacaa
    10381 tcgtttctga gtaagccatc cctccattct gatcattttt cccaacattt tgcgctctcg
    10441 ttccaagtgt taaattgtgt acctccatat ctcctcctag cgaccatgct aatgaatgac
    10501 ttttgttata gagataacct ttatagcctt ctagctgagt gatttcattg tttgaatctt
    10561 tgccgaccca agtatctctc tcttgatcaa atactccatc cttgtattct cctgcaatat
    10621 ggacagaact tttgaaagat ggacgttcca catttttaga tgagtgagaa tttacagcat
    10681 cataggtgat caacgcttca acatctcctg ctcttccaag ttgatctaaa tctgcataat
    10741 ttacccaaaa ggtttgactg cctttttctg ataatgcttc atggtcttct tttgtcagtg
    10801 tatatttccc atcattaatg tttacgatat tatttggata tttgtttgga tcataacgta
    10861 tccctaaaaa ttcttttgat tcactgtctg ctttattacc tacctgaaca ctacttttga
    10921 tctgaaaatc atgttcctga gtactgttat tctgaaagta acccttgcca ctaagaccaa
    10981 ccgcaactaa aaccaaaatt cctaacaacg atcggctaaa cttcttcttt ttcataccac
    11041 cattcctttt attgtgttat cataataaga taaatatata ataaactata ttgtaaattt
    11101 tgtcaaatgt atttttattt attcccacta taaaccttga tatatagcca atttaaaaaa
    11161 ggatggcaaa aaattatcaa gttaaaaaaa taaattttga accaaattat ttttaataaa
    11221 atagctagtt tttttatcaa aaaaaataga gcttttcagc tctattcaga ctgtagacaa
    11281 actcctaaat ttggagtgtt gtttgcagtt ttttgtttta tactaattgt ataaactatt
    11341 ctgggagtga ttctaatgat gagaaaacaa tcgattgaag gtagaaatca atttgctatg
    11401 ttaacaattg atgatcttgt acctaaagat cacttagttc gaaaaattga tgctgcgatt
    11461 cagtttgatt tcatttatcc cattgtagaa tctacgtatt caacctatgg tcgtcccagt
    11521 attgatccag tggtcttaat taaattagta tttatccaat atttatttgg tatccgttcg
    11581 atgagacaga caattaaaga cgttgaaaca aatgttgctt accgttggtt tttaggttat
    11641 tcattcgagg atcctatccc acacttctct acttttggta aaaactatgt tcgaagattt
    11701 agagaaacaa ctttatttga agatattttc tctcatattc tagaacaagc tgtaaaagct
    11761 ggcttcgtta cggaagacaa cctttatatt gattctactc atatcaaagc aaatgccaat
    11821 aaacataagt tcacgaaaga gatgacctac ggtcaagcaa aagcttatca agatgaatta
    11881 gaagatgaaa taaataaaga acgtattgct gtaggaaaac gtccttttac atgggacaca
    11941 gaaagtgagt tgagtcttca aaaaataagt cattcagatc cagaaagtgg ttactatgtt
    12001 aaaggtgaac gagaaaaaca atttgcttat tctgcacata cttcctgtga agataacggt
    12061 tttatcttat ctacattaat cacaccagga aatgtgcatg acagccaagt cgcaatcgat
    12121 ctggttaaac agtcgaaaag agcttttcca aatataaagc gtgtggttgc tgatgcagga
    12181 tataagacgc ctaaatttgt tcattttctg tcacacttga aactctaccc gattcttcca
    12241 tatactgcac ctcatggagt gaaaggaatg tttcgtaaaa aagactttgt ttatgatgcc
    12301 tattttgact gctatttatg tcccaataat caaccgttgt tattttcaac tatcactcgt
    12361 gatggttatc gaacctataa atcaaatcca aaattctgtg tgagttgtcc attacttgaa
    12421 aattgtactt tgagtacaaa acatcaaaaa atagtgactc gacatatttg ggctgatctt
    12481 atggatgaac ttgaacacca acgccatacg gcgttgaata gagaaattta caaaaagaga
    12541 aaacaaacca ttgaacgaat atttgcagat gcaaaagaaa agcatggtat gcgttggacg
    12601 aaatatcgag gtcttgaaaa agtcgccacg cacacgatgc ttacgtttgc tgccatgaat
    12661 ttaaaaaaac tagccacatg gctatggaaa acaaaaaagc ctatggcttt ttatttcaaa
    12721 attatgcaaa gactaacaaa aaggagtggc ttaattacca actccttttt gtctacaatc
    12781 tgaatagagc ttttcagctc tattcaatat cattttgatc aattaataat cgtacactcc
    12841 aattaaccga gttttgatca ctcaaatatt tataatcttt gtcatcaaaa accagttgcc
    12901 aaactctggt acgaacagtt tcttcgtcca cttcctcatc tgaagagtat tgcgataatg
    12961 cttcttcaac tatatacttt acaacttcct caaaatcagt aggaacttta tcaataacca
    13021 cttcatagtc ttcttgtttt acgatattgg gatcaatatg ttcatccaaa catacctcag
    13081 tgagtttttc tttcacttct gtatctcttt ctgtttttag gtactctgca tagtcttcct
    13141 tagaccattg tttttcaaga tcatccgcta tacttttaat aaaggattct ttatttaacc
    13201 aattatctgc ggtatctata ccttcaaact caaaagctgt tccttctata cgtgatttga
    13261 cgaaagcgtc aattttcttg tattgattat tcatttccca tgaaacacac tcatcttctt
    13321 gcgtaatatc ataaattttt tcggaaaaac taactaattc attccacgat ttatcctcgg
    13381 caacttgact tagtttcgat gcaataaaag gaaattttgc ttttaagtca tccatattct
    13441 tgatctcaaa ttcgatattt tcttctccta cagctgaaat ccatgctgaa agattgattg
    13501 caatatcgct aaagtcttct acattttcga catttcctgt attatctttt attactgctt
    13561 ctaaagccat atcggttgtt gcactcatgg tttgatctcc tctcataata gagcaaaaag
    13621 ccggtttaac cgactttttg catggtacgt tttgtttggt agttttctgc ttgtgtttat
    13681 gttgaaataa tccctatagc tcttctttta atagttcttc aactttatct cttaactctc
    13741 cctgaccact ctctaataga tttaaatagc tcatcagaat atccgtttca tacgttttta
    13801 attcagctct atactcactt tctgaatatg atcgtaaata cccatcattg ctaaatgcaa
    13861 ctagatcatc ttgaaagttc cattctccta aatttgtttt acttactgct tcccatggac
    13921 tattactaaa catttcatta aagaaatctt cgtcattttg atataccttc aattcaggaa
    13981 aatagttgct gtcttcattg atttcttgca ccatttttcc aagttcttct tcagataatt
    14041 taccttccaa taattttact ttttctctgt catttgcata agccatttga acctctcctt
    14101 tttctttttc aatttcttca ggtgtttgtt ttggcgtaac tttcaaatct attccattgc
    14161 ctattagacc tattagataa ccatagaacg ttttaaagtc acttgtagca agagatttgg
    14221 catcagggta tgtatcccct ccaattttat ctaaaacaaa atcagcaatc gttacccaag
    14281 gagaatatat cgtacatact acatcccatg tagcatcttc aatattcgtc catgtcggaa
    14341 agtcattttc attcaaatag tcttttttca ttgtttcgat cagagttttt ccactttcgt
    14401 tttggcttag atattcaaca aattctgcac catcaaacct gctactaaat agtttagcta
    14461 atgagtctaa agctgttttt gatactccca ccattactac acctcctatt aatattttac
    14521 gatgacttgt ttttcattca caaataagat tttctacatc ttatctattg ctttatagct
    14581 tttccataca ttatttctaa atacaaccaa taaccctatt aaattaagct tttgagttta
    14641 tcttttctaa taatattctg ataaatattc tgataaaaaa atttattgta tttttttctt
    14701 gactttttct ttagaattaa gatcagtttt gattttattt aaaagtgaat ctagctctat
    14761 tcaaaataaa aaagagatca ataatagtat caatcccttt ttactgtact ctatcaattt
    14821 ttataatctt cattatgctt ttcgaaactc tttgattaaa agaataagaa cctttagact
    14881 tgctaatgct cctattacag tccaaatgac taaaagcatt ttctgatgtt tattggtaat
    14941 tcctgtaaat attgaacttg ccttttcagc aaacgttctg tcttgcatct ctacgctacc
    15001 ttgttccgga atattaagat cattatttcc atctaattcc ttgttggcac gatcaacatc
    15061 ttcaaataaa tcattagaag atgtaacagt ttctgtctta tctgaaacgg actcgctatc
    15121 aatggcatca gcgtatgcgg tattgccaac tgttacttgt ggcgtgatgt attttgcttt
    15181 ttcatctctt agacttctaa ctaataaacg atcgtagttc tgaggatatg gatgacaagt
    15241 ataaagtgtt aacatatcta aatcaggaat atcagtcagt gttgaattct gaccgtaatt
    15301 tctaataacg tctgttcgga aaaccgtata aactgctttt tcaccaagat aatctatata
    15361 aatgtggtct ccggctacta attgatcaac ataaagaaat tgtggtgacg tataaccgcc
    15421 acgatgacct gctattactg tttgatgacc tttacctgat aaaggtagac tagtagcact
    15481 catttgtgcc acacctctat ataaatgctc ctgtgaagcg cctagaaaca aaagctctag
    15541 cacatttatt tttggaatat agacataacc aaccgctcct gaataggtag atggttcaaa
    15601 atctttcgtt tgattttttg aaaaaacatc tcctgtgtat ggattgacct ttccttcagc
    15661 ttcacgaata tcaaatgatc caccgtctgt ttcctgagta ttgtctactc ctttatctgt
    15721 tttctgagta ttgtctaatc cttggttacc gctgttcgat tttatttgtt cgactttttt
    15781 tgccacttcc tcttttgaag gagtttgttt tttctcaatt ttctcttgga tttccgccac
    15841 attctctttt gttttgaatt gatgatagag ctgatttgca ggataaattg ttaaaaccaa
    15901 agtgcaaata agagtcaaaa aaagtagtat tttattcttc atttttcatt cctcgtctct
    15961 ttgctactat ttttttaata atgactatca cagctattat taataaaacc cctacaacta
    16021 cgataggata ccagttcatc tgcgccttta cctttaaatc ttctttcttc ttttctttct
    16081 caacaatact tttttggtaa ggtatgctaa cccctctaac gagtaaccta tgagaattat
    16141 ctccaacagg tgtacatgtc ataagtgtta cataatcttt tcctgccaca ggaagcaaat
    16201 cctcaaattc ggttggtaga attgttttta tccgatctac ttgataagca agttttttcc
    16261 ctgaaatatc aataaaaaat ttatcaccaa gtttgagcaa ctctaaatct gtaaaatatt
    16321 ttttattagg aatgcctgaa tgagctgata taacagaatg gtttccaatc ccaccagttg
    16381 gataatcagt tccggttaat attcctgctc ctgctgatag ttggaaatcg tttacaccat
    16441 cataaattgg caaatccaca ttaatctttg gtataagaat ttcacccact aattcaccta
    16501 atttatcctt ctgaagattt ccatttgacg gaatattgtt attatcttca ggaatcgtga
    16561 ctgaggtttg ttctgtgtct tttcgtttcc tattaacttc tgaatatctc ttgaagactt
    16621 cttcattctc cttttcagaa gcttcatcat atttttcctt ataggagttt agtgtttgtt
    16681 gagtttggta gtaggtataa gcctgagtaa ttacaggata tagacctact cctaaacata
    16741 gcaacatccc aatttttaag atgatcaact tgatattctt ctttttcatg ctcataccta
    16801 cttttcacac actttttaaa aagaaaccct agagccaaaa acactctagg gaattttatt
    16861 tcattaagct ttgtgttgtt ctcgtttgta gacaacatat agccctgttc ctgccacacc
    16921 tgcaaccact agaaatacta ctgttccaat tccacctgta cgaactgttt caggtgtttt
    16981 aaggttcttg atgtctgtta cttcgctaga agcatcatca ttcgatacca caaatggaac
    17041 atcatttcta gttggtgtat atcctgaagg agcaacaact tcttcaagag cataatcatt
    17101 gattttttta tctgtgattg ttggattacc ttgtttatca aaatctacat cataggtata
    17161 ttgcaagcct tcaatttgga acttaccatc tgatccggaa acgaattcag tagctttaga
    17221 tttatctgca atccaagtaa cagatttcgt tgttggatct tgttttaggt aagtaacttt
    17281 attcggatca attttgttct tcactacaaa ttttgctcca ctcaatgctt tgcttgtatc
    17341 tccatctgtt ttaatgaatt ggtgatcacc aatgattgta taagcgtgat caactggtgt
    17401 ttcgtcttcg ggttcatatc cattgttgta gatcgttgaa acatcattct taagtggtgt
    17461 atccatttta gctttactat tagtatgagc gttcatcaaa attgtaaaga agcgatcccc
    17521 tgcatcaaat tgtttctgaa gcttatccat acctgattca gtaaagacat agtttgtcgt
    17581 ttgtccgtca cgttcgatac tgtaatcagt atctaaagtc attccttcga tcacttctga
    17641 tgaatcataa gttaactctg cacctagaac atctttgaca acatattttt cgtaattacc
    17701 aatattacca ggaattgatg gtttcaatgt atattgaaca tcttcgccag tatcaacggt
    17761 ttctttatca attaccttag taattacagg tcgtttgtag ttggtaacat caagaactac
    17821 tttagcagtc tccccacgtt taacgctaaa tttgaatttt ttaccatcta gtaaataggt
    17881 tgttttatta gcatctgttc ccaaatcggt ttctaggaaa taatagtcac ctaattccaa
    17941 accatctaca gttaatttac ctgtgctgtc tgttttataa gttccaacaa cctgatcaga
    18001 tccatcatct tttcgcaatt caaaaacagt tccggcaaga gcctttccat cttcgtcttt
    18061 tttatcaagc tctgctgagt ttaattggaa atagttttta ggatagacat gtacatcata
    18121 attaaagtat tgatcatctg atccgtttag ttcaggtacg ttaacaatca taggatacgc
    18181 ttcttcaact acacctttga ctgttcctgt ttcaacaatc aaatacaagt tatctgttaa
    18241 atcgtctgta tttacttgta taggtatgtc tatattccct tgtgaatcag tcttcattct
    18301 acttacttca ctgtattttt tgtttagtaa taatcgtgct ttagcatcat caatatgttg
    18361 accttttgta gtatcactat ccgtccatcc caaggattta gcttcatctt tagtaagttt
    18421 cacataagag aactcaacgc cttcaagagg ttttacacca tttggtaaag ctatttcttt
    18481 ccctttgtta tcaatatctg agacagggtt cccatcccca ttagtatttg ttactttatg
    18541 gatatgaatc gttccagttc cttgataatt atagttttgt tgtgcatatg ctacttgccc
    18601 ccctagtcca aggctagaag agattgagct tgcgcccaat cccactccta gcaaagtacc
    18661 aaatagcatt aaatttgaaa aaattttttt tgatttcata cgtcagcctt cttttttcat
    18721 atttacttct tttccattaa cactttttta ttttgctttg tgctgttctc gataatacag
    18781 tagcaccata ctcaatcctg caattagaaa tacaagaagg aaggctgatg taccgattcc
    18841 gcctgttctt actgtttcag gtacaaggtg attaattact gtatattcaa aggtttgtcc
    18901 atcgtcagca acggtgatgc cttctttaga ccatttccct gtataaccat tagggttttg
    18961 tgtttcttcg attttgtacg ttttgttggc aacaatatta aatgttgcca agccattgct
    19021 gtccgttttt tgtacaactt ttacaccttt gtcgtctgtc aaagtgaatt ctgcgtttgc
    19081 taatgctttc ccttgatcat ccactttatg aactttgatc acaccttttt tcaaagtgtt
    19141 tttagcagta tattcaaaga cttgtccatc atctttgacg gtaattcctt cttgtttgaa
    19201 tgcgcctgtg taacctgttg gtacttttgt ttcttctaag ttataagttt tgttcgcaat
    19261 aatgttgaac gtagccagac cagttgcatc cgtcttttta acttctttta cacctttttc
    19321 atccgttaag gtgaattctg catctgctaa aggttttcca tcttgatcga ctttatgaac
    19381 ttttactaca ccttttttca aattatttgt agctgtatat tcaaaagttt gcccatcgtc
    19441 tttaacggtg ataccctctt gcttaaatgc acctgtgtaa cctactggta ctccggtttc
    19501 ttctaggcta tacgtcttgt ttgctacaat attgaagctt gcaagtccat tttcatctgt
    19561 tttttctact tgtgttgcac ctttttcatc ggttaaagtg aactctgcat ttgccaaagg
    19621 ttttccatct tgatcgactt tatgaacttt tactaaacct tttttcaaag tgtttgtagc
    19681 tgtatattca aaagtttgtc catcatcttt aacggtaatt ccttcttgct tgaatacgcc
    19741 tgtataacca gttggtactc cggtctcttc aaggctgtag gtcttgtttg ctacaatatc
    19801 gaagcttgca agtccgtttt catctgtttt ttctgcttgt gtttgggatg aatcatccgt
    19861 caaggtaaat tcagcattag ctaatggttt tccatcttgg tcaacctttt taacttttac
    19921 tttacctttt ttaattgtgt tcgtagcagt gtaactaaat tcttgtccat cggtattaac
    19981 agtaatacca tcctttttaa atgaaccttg ataaccttca ggaacttttg tttcttctaa
    20041 gctgtatgtt aagccttgtt gcaaatcaaa cgtagctaac ccatcatctc cggttgtctt
    20101 aacagattta gcattgttat tgtcagttag agtaaactcc gctcctttta aagcatttcc
    20161 atcttgatca actttcttaa ctgttacctg accaacagct cttgtccatt tagcactcaa
    20221 agatttagag tcctttgttt ctgaagtagt gatcataact acggcatctt gatacacacc
    20281 tcgtccatca aaagtaaaag gattggttgt gctaacatta gcagtacctt taaatgacaa
    20341 agtaccactt ggtgtagtca caggtacttt aatagtaaaa cttgaattta atttcacagt
    20401 gttgttagta atttctttac catctgcatc ataaatcttc ataccagctg gttttgctga
    20461 tgtaaaagtg agcttcgcat taccagtctt atttgatgct gttttatatg tgaaagtgtg
    20521 atattttcct tcgtcagctg tcttagaagc tgtgatagaa aactcagtgt tgggcgtatc
    20581 ttttccattc ttagcatagt cataaatttt ttcaaatgct ttttttacac gagctgtttc
    20641 tgcggcggta tggtttggat gtgaccatac aatctgtgaa actttaaagt ttccggctaa
    20701 aacccatgaa actaattgtg ttgcatacca tgcttctgta tcatttcctg cacccagttc
    20761 ctttgctgtt tttgatggaa aaccaccttt aaacatacga tagaactcat cactgccagc
    20821 tcccttgtac ttcaagcttc cggctccact tcctgttggg gatggcaatt ctgaatccat
    20881 acaatatcca atatttccat gagaatcttt aaattgactt gaaatcatcc attcatttgg
    20941 atgattcggc atattaaccc taatataacc gtctgttttt acgccagttt ctataaattt
    21001 tgcacttgtc ggttttgtga catctgcttc agctcttatt gctgaaaaag gcattataag
    21061 cgtagataac atcattacta aagtcgcaaa acaataaatt atttttgatt ttaaaattat
    21121 cgttttttca tgcttcatct ctcgttactc cttctttata ccagttaggc gaagaaaatg
    21181 agccacgata tgtcggtgtg gtaatgacct tcgcgcccat tcctaagtta aaatcaccac
    21241 acctattaat catttttttc gttgaaagca agctgtttca acgctctagt attcatagca
    21301 tcaccccttt tagttctacc caccatgagc aacaagtttt ctaagtagtt aagcaaggac
    21361 aaataaagac tatataacta cttagtatat aaacgatatc ttttattaac gtaccgaaac
    21421 cgctaattga gatacgttat atgctgtttc aaatgatcct gcattaatcc aacttgcccc
    21481 aaacacagga tcgacaacat ggtaaaaacc gtctttaaat ccatcaacgg ctactacatg
    21541 tcgattccaa atagcatcgc cccaaaacat tggtttatgt tgagcaggtt caaaggcata
    21601 agttacccac gctactacag gatttccatt tacaatctca tcttttaatc cttgtaccga
    21661 tgaaccagaa atatctacaa catttccgcc attagcaaca ccccatgaaa ctacaggagc
    21721 cggattcata ccttgataag ttccatccac attgtgtctc cattcaccag aaaaaccatt
    21781 atttggatta ttgtcaggag atagtggcat tgatttaaca aaagtcatta aatcttgatt
    21841 tgttgcaaca cctttataat gtaacgcttc tagcaaagat gttccttcgc aaagcattgt
    21901 gttaccttga ctaataaatg gaacgcctag cacaacatta gttggttgtt cgggtttact
    21961 tggtgaagta gaactgtcag tttcatcact cgaacctgtt tgtgattgat cactagaaga
    22021 tgtactggtt gattcatttg catcatttga tgttgattgt gagttatttg ttgatgttcc
    22081 agtatcagct tccgtagtgc tactttcact tgttccctgt gatgcttctt tctcacttgt
    22141 agaattattt gtattagatg aacttgctcc atcaacactt gaagcacttg tttctgtctt
    22201 atcagaagtg tttgctgttg gttccgattc ttttgtagtc tcggattttt tgttctcttg
    22261 ctccgtattt tcaataggat caggtttatc tttttgaaca gtttgactaa ctacttcgcc
    22321 atctgcttcc ttaactgtta atgtatcttt actgatgttg aaagttcgtc caatcgggat
    22381 agtccgttgt tctccttttg ccaaatcaac attgttcaat tcggcaaact cagcaacagt
    22441 agtcttgtaa cctaagcttt taagcttctc tacagttgac caaatagtat ctcctttttt
    22501 gaacgcataa cttgattgtc ctgttgtaat ttggatttga tccggtgtgt ttgcagtcca
    22561 ttcatcagca tgtgaggtta ctggtactga taaccccgca agtacagctg atgccaataa
    22621 aaccatacca agcgaatttc tcttcatatt cattccttct ttttgttttt attttctgtt
    22681 tgtgatcgca atgcttctat tccttcttcc gttataatat aggttttgtc acccactttc
    22741 ttgaacgaac ctttaggcaa acgatttgga taatagttga ttatgtttgt aacataattt
    22801 ctacctttac ccaaaatcaa acttgcttcc cttgcggtat acaagttgta tttgtctagg
    22861 ttcaatttct ttttgtttgc gatttagacc tacccctttc gcaaaattta ttgtataata
    22921 atagtaccat tgcactgtca taattgcaat attttttggg gtttatcttc atttaatagc
    22981 ctaaaaaggc ttgttagata aggaatatct aactaatcaa aacaaaaaag gatgttaaga
    23041 aaaaatgaac aaaaaaagga aattatcagt attaattaca cttgtttcta tattgactat
    23101 tcttagcgtt atcttgtcgc tgaaaatatt caatcaaata aacacaagct caaatgccaa
    23161 agatggaata gtcgtagaaa atacctctaa ttctaaagaa actggtacta ctacaaaaaa
    23221 atacaaatct gctgagatca ctacgaaaaa ttatttaata aagactgttt attttgatga
    23281 acgaaaaaca gcaattctca aagatgagga aggatcagaa ttaaagctac ctataacggt
    23341 atccgataag ttcaatggaa cgttctcctt acaaaccgct gaaggatcac ctgacaatct
    23401 caaagttggg caaacaatca ttcttggtaa aaacgacgaa ctgtattcat ttgtaaaata
    23461 aaaaaatgga gcaaaggcta gtcaagccct tgctccattt tccattcttc aagaggtgta
    23521 gctatttcaa caccaaagtt taaatactgt ggatgctgta gatacatgat atccattcta
    23581 ttttcgataa actcttttaa cgttgagtag atgtattttt ctaatgctat atctgcccaa
    23641 ctaccataat caagtttatt cgtgatcttg acgtttattt caatttgaga aacatcaata
    23701 ttttgaacat caggaaactt attcagtctt agctcttctc taaccgcttc tactagcggt
    23761 tcatattgtc cgcctagttc gctaattgtt gaaactattt catctgaatt ccaacgatct
    23821 tccaatacct ttgctacatt ttctttaggc ggttgtcttt ctataaattc ttcaggtgtt
    23881 tcatcaaact ctactctaaa gttcaattta ttttgattgt tagcatacat ataatcatct
    23941 ctgtttgatt caaaaaagtt acgaattcct gtgtctgatt tatcacttaa ctccttattt
    24001 gctaaagtag cccaaaaatc ccaagttcct ttattttcag tatacaaagt aaaattaatg
    24061 tttatatagt ttgcagtttc aggataccct tgctctctaa gagcatcttt aacaatcact
    24121 gctaagtcag tttcaaaatt ctccataaaa aattcactaa attcttttgg attccacgtt
    24181 tcttctaata acttttctaa tttatgatct gccattatta ttctccccca ctttactttc
    24241 aatcttatat ctaaaactgc ttaaaattaa gagctatacc ttatcataaa aggtatttta
    24301 tgctttattt aagtctacaa ttctatatt
//
LOCUS       DAXWLH010000029        24306 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXWLH010000029 DAXWLH010000000
VERSION     DAXWLH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.029"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..226
                     /locus_tag="ACGE36_000832"
     CDS             <1..226
                     /locus_tag="ACGE36_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383250.1"
                     /translation="VAEVQKLGKSMWTEKPKRRTPRKIAIWHDDRILVTGTAEQLSEI
                     TGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            226..582
                     /locus_tag="ACGE36_000833"
     CDS             226..582
                     /locus_tag="ACGE36_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFF8383251.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            542..787
                     /locus_tag="ACGE36_000834"
     CDS             542..787
                     /locus_tag="ACGE36_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383252.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            784..1203
                     /locus_tag="ACGE36_000835"
     CDS             784..1203
                     /locus_tag="ACGE36_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFF8383253.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            1200..1757
                     /locus_tag="ACGE36_000836"
     CDS             1200..1757
                     /locus_tag="ACGE36_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFF8383254.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1754..2050
                     /locus_tag="ACGE36_000837"
     CDS             1754..2050
                     /locus_tag="ACGE36_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383255.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            2127..2540
                     /locus_tag="ACGE36_000838"
     CDS             2127..2540
                     /locus_tag="ACGE36_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFF8383256.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2849..3001
                     /locus_tag="ACGE36_000839"
     CDS             2849..3001
                     /locus_tag="ACGE36_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383257.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2998..3273)
                     /locus_tag="ACGE36_000840"
     CDS             complement(2998..3273)
                     /locus_tag="ACGE36_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383258.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            3769..3933
                     /locus_tag="ACGE36_000841"
     CDS             3769..3933
                     /locus_tag="ACGE36_000841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383259.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            4129..4296
                     /locus_tag="ACGE36_000842"
     CDS             4129..4296
                     /locus_tag="ACGE36_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8383260.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            4322..4666
                     /locus_tag="ACGE36_000843"
     CDS             4322..4666
                     /locus_tag="ACGE36_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFF8383261.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4671..4952
                     /locus_tag="ACGE36_000844"
     CDS             4671..4952
                     /locus_tag="ACGE36_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383262.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            5055..5369
                     /locus_tag="ACGE36_000845"
     CDS             5055..5369
                     /locus_tag="ACGE36_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFF8383263.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5347..7041
                     /locus_tag="ACGE36_000846"
     CDS             5347..7041
                     /locus_tag="ACGE36_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8383264.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            7061..8239
                     /locus_tag="ACGE36_000847"
     CDS             7061..8239
                     /locus_tag="ACGE36_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8383265.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            8202..8888
                     /locus_tag="ACGE36_000848"
     CDS             8202..8888
                     /locus_tag="ACGE36_000848"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFF8383266.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8888..10048
                     /locus_tag="ACGE36_000849"
     CDS             8888..10048
                     /locus_tag="ACGE36_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8383267.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            10058..10933
                     /locus_tag="ACGE36_000850"
     CDS             10058..10933
                     /locus_tag="ACGE36_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383268.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFLEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYRKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10930..11241
                     /locus_tag="ACGE36_000851"
     CDS             10930..11241
                     /locus_tag="ACGE36_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383269.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            11231..11584
                     /locus_tag="ACGE36_000852"
     CDS             11231..11584
                     /locus_tag="ACGE36_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8383270.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11574..11975
                     /locus_tag="ACGE36_000853"
     CDS             11574..11975
                     /locus_tag="ACGE36_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383271.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11968..12372
                     /locus_tag="ACGE36_000854"
     CDS             11968..12372
                     /locus_tag="ACGE36_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383272.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12384..12992
                     /locus_tag="ACGE36_000855"
     CDS             12384..12992
                     /locus_tag="ACGE36_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8383273.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPNNMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            13012..13374
                     /locus_tag="ACGE36_000856"
     CDS             13012..13374
                     /locus_tag="ACGE36_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383274.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13377..13559
                     /locus_tag="ACGE36_000857"
     CDS             13377..13559
                     /locus_tag="ACGE36_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383275.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13577..16897
                     /locus_tag="ACGE36_000858"
     CDS             13577..16897
                     /locus_tag="ACGE36_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8383276.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            16948..17685
                     /locus_tag="ACGE36_000859"
     CDS             16948..17685
                     /locus_tag="ACGE36_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8383277.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17695..19986
                     /locus_tag="ACGE36_000860"
     CDS             17695..19986
                     /locus_tag="ACGE36_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFF8383278.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            20010..22232
                     /locus_tag="ACGE36_000861"
     CDS             20010..22232
                     /locus_tag="ACGE36_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383279.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVASGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDAPHYLSKTPLGENIANKSVTF
                     PINTSAYKLYEYNTEEDLIIGQTYTITLKGTKPASQTFAVYNYWNINLGSLKPVEGLT
                     DVWSITFTPTTLEPGLPKDLRILQSPKETAGACQIDWLKIEKGDTRTPNIEQYKYRGI
                     GMRDSNNPKDYVWDLAPEYVENNLATDIKVAEITGKANNYTDGKVSQINSQLTASINE
                     VDTTAKGAQTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGT
                     GYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGRKYASWLYTDLYKDGTSVD
                     NLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD
                     "
     gene            22310..22552
                     /locus_tag="ACGE36_000862"
     CDS             22310..22552
                     /locus_tag="ACGE36_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFF8383280.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22568..22765
                     /locus_tag="ACGE36_000863"
     CDS             22568..22765
                     /locus_tag="ACGE36_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8383281.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22776..23795
                     /locus_tag="ACGE36_000864"
     CDS             22776..23795
                     /locus_tag="ACGE36_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8383282.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            23866..>24306
                     /locus_tag="ACGE36_000865"
     CDS             23866..>24306
                     /locus_tag="ACGE36_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8383283.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAY"
BASE COUNT         8595 a         3653 c         5168 g         6890 t
ORIGIN      
        1 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
       61 gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta ctgctgaaca
      121 gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta ggagcttatg
      181 gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga tctcattaca
      241 caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga taaaaaatca
      301 aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg agtgcgaagt
      361 agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat tcaaaaaggg
      421 ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact tcatcaagat
      481 cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag gaggacagca
      541 aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat caggagcaac
      601 actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt
      661 gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc
      721 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      781 cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc
      841 gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      901 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat
      961 aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc
     1021 gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc cggattagtt
     1081 tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa
     1141 tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga ggaacagcga
     1201 tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga aatgatgcaa
     1261 tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa aaaatagacg
     1321 aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa tctacggacc
     1381 cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt gattcatatt
     1441 atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga gaagaattac
     1501 tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac ggctacgagg
     1561 ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt actaaatgtg
     1621 ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct catcgattgc
     1681 ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga acagtggaag
     1741 aggtggtaga aagatgaaac taaaagacgg attttacgct agcagtcacg gtatcggcgg
     1801 tttaatgcta gatatgccga caaagaaccc taaaacacgc aagaaatcaa aattcaaagt
     1861 cggtgacatg gtccgctgtg aagcagaaga gttcgtttat ccgttcagag gatacgttaa
     1921 aaagatactg tcaaactcag caatcattcg tattgaaaat acaatgaagt gtgatcgttc
     1981 gactgcaaaa cacaagcatt atttagctgt agcgagatta gttgacatgg aagtaatcaa
     2041 gagcaaataa aaaaagccgg atcgctccga ctgattcaat aaatccaaca catttattat
     2101 atcacataaa ggagcggttt gacttgatgc aattgttacg agaggtagat ttcaaacaga
     2161 caagatgtaa tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc
     2221 gctctttgat agatattaag tcgccgatta taacggatat gccgaaggca ccgaagcatg
     2281 gcaataaggc agaagacgcg attattcaga tgatggatat agaaacggag agagatgcga
     2341 ttctagcggc tttgatggct cttagtctga ttagtcgtca gatactctac tacagcttct
     2401 gtgtgccaga tagcttctca aactacagaa ttagccgtga agtgggttat tcagaaagaa
     2461 gtatacaacg gatgaagtcg gaagctctaa tagagtttgc agaagcatat aaacacggaa
     2521 aaataattgc ttataaataa tttggcggtt ttttggcgga atgatggcgg tttttagaca
     2581 tttaccagtg ttattatggt agtgtcgaaa gattaggaaa caggatcgac aaaataaacg
     2641 aagggaggca atctccctca tcgttttaaa cttcttctta gatagacagc aactaattat
     2701 gaagaaaagg atgtgaattc aactcctcct aaattcttca tcggttgctg tttattttga
     2761 ttagacagca gcactcacaa aactaaacat gttcttgtcg tctttcaact gctgctgtct
     2821 attaatttat gtattggagg gaagaaaaat gaaattatca gtagaaggca ctccagaaga
     2881 aatacaagaa ttgctccaag ctattggtag tagcaaggag caaaaggtaa gtgtttctaa
     2941 ttcagatatt gacggtattt gcaattgttt aaacagagaa aaaatatcta atccttacta
     3001 agagaaaata tgttcgttaa tgaatttcca attttcctct gtagctctac cttgataatt
     3061 tttttgcagt tcacagatga ataaggaatc gttattatca agtactgtat ttaatttttc
     3121 acacatttga tttggattca aattagtttt aactaaccaa aaagattttt gtagctttat
     3181 atatgcccca aattctttta ttatttcaaa tactttatca tacttttgcc ctgggttatt
     3241 aagatcataa ctaatcatgt agggtttata catgttgtct tgcacatcct tgttttatat
     3301 ttcagcggac cactcgctga taaataaaat tatacgctta gtatttattt tcacaatatt
     3361 aatttatcgt aataactttt gagtaaatag aacaaaaaac ctgcactagt ttccgctagt
     3421 acaggcagtg actatatcgg ttgataatct agcatatatt aaaaataatt gcaagaaaag
     3481 aaatttatag tggtttatac caaattatca aagcaatgtt attttgttgc tgtctattgt
     3541 tttttaatta ttcacacgat aactaaaggt gggtgaagag aaatgattcc attaataatt
     3601 tcaatttttg cgctctgtct taatgtctat atgattggat ttaaaaatgg gcaaaataaa
     3661 aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa
     3721 gcatgtgtgc taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagcgaca
     3781 ttaaatgcct atgacggtta cgactaccga aaaaagatcg ttaagaagct atacggtgct
     3841 acgtacggca atgtagtaag tgtgctatct gtacaccacc aagcttcggt cactgtggcg
     3901 gaagtagaag acgcagcggt aaatggcgag tagcctcgtg agagcctggt aagttctcgt
     3961 gagtggtgca atcccactcc agcgacttta agcaaccgag gcatcggcgg tttaaaaata
     4021 taggggtgcg caatttcgta cgcgttttgt gcatcgtgca agttactatt acatattaga
     4081 tcactctttg agtggtcttt ttatttttgc acaaaggagg aaacaacaat gtatagacca
     4141 caatacttag aacagaagta tgaagtaatc actgttcata aaggtaatgg cgaaaaagta
     4201 tatgagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa caatgaagtt
     4261 attccattgt atggcaaaag aatagctaag cattaaataa gattgcgaaa ggagacggaa
     4321 catgaccgag gaattctata gatggctatt acagttgaca agagaagatc gtttggttaa
     4381 gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta
     4441 tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat
     4501 aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg
     4561 tgttgaacat cataatgaag ttcatggcag atatcttaca gcattagata aacaagagaa
     4621 gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca
     4681 atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg
     4741 gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata
     4801 actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg
     4861 tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat tacattgact
     4921 tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt
     4981 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
     5041 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
     5101 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
     5161 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
     5221 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
     5281 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
     5341 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
     5401 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
     5461 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
     5521 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
     5581 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
     5641 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
     5701 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
     5761 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
     5821 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
     5881 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
     5941 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga
     6001 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
     6061 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
     6121 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
     6181 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt aaaacggaaa
     6241 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
     6301 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
     6361 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
     6421 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
     6481 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
     6541 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
     6601 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
     6661 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
     6721 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
     6781 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
     6841 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
     6901 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
     6961 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
     7021 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
     7081 ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca
     7141 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
     7201 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
     7261 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
     7321 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
     7381 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
     7441 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
     7501 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
     7561 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
     7621 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
     7681 gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg tatttcaatt
     7741 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
     7801 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
     7861 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
     7921 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
     7981 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
     8041 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
     8101 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
     8161 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
     8221 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
     8281 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
     8341 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
     8401 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
     8461 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
     8521 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
     8581 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
     8641 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
     8701 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
     8761 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
     8821 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
     8881 taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
     8941 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
     9001 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
     9061 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
     9121 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
     9181 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
     9241 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
     9301 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
     9361 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
     9421 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
     9481 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
     9541 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg
     9601 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
     9661 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
     9721 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
     9781 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
     9841 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
     9901 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
     9961 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
    10021 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
    10081 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
    10141 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta tttttcttga
    10201 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
    10261 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
    10321 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
    10381 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
    10441 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
    10501 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
    10561 taaccgtcct aaagcgaatt atcggaaaaa ggtttatctg gattctaatc ggctatatcg
    10621 ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt gggcacagct
    10681 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
    10741 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
    10801 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
    10861 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
    10921 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
    10981 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
    11041 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
    11101 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
    11161 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
    11221 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
    11281 gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac
    11341 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
    11401 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
    11461 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
    11521 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
    11581 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
    11641 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
    11701 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
    11761 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
    11821 gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
    11881 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
    11941 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
    12001 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
    12061 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
    12121 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
    12181 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
    12241 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
    12301 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
    12361 ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca acgttcgata
    12421 acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca atcgcaacga
    12481 attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag
    12541 cgacagaagt aaaatcaaaa tctaaaccaa acaatatgac ggtaacaatt actgcacatg
    12601 taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt attaaaccag
    12661 gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct gcagagatcg
    12721 tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact tcgaatacag
    12781 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    12841 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    12901 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    12961 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    13021 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    13081 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgtcg
    13141 atgggaaaag gctctatgga tattacagtt attcaaggtg caaaagccgt atatgtagct
    13201 tatcgtcaag cgaacatggt cgattacatt tcattcgatg aatttataga taaatgggat
    13261 tttgatatgg aggttgcagt agctgtatac agtactatga tgttcaaaca agcacgtgat
    13321 gcctatcaaa aagaattcga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    13381 caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    13441 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    13501 acaagagcgc ttatgaagga tggaagaact atgcaataaa tcaatcccag aaaaactaaa
    13561 ggaaaggagg taaaaaatgg ctaaaaagag aacggaagca gaagtaactt tcatagctaa
    13621 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
    13681 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
    13741 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
    13801 tagtcaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
    13861 acttgaaaga gaactgatta atcaacaaac agctcagcaa aggttatcta atgatataga
    13921 taaaacgagt aaggcattgg ctcaatctaa aggtgaattt aaaacgtatg agtcaactat
    13981 gaaagactta gataatgagc aacaacatct aaagtcaagc gcatcattag tagaatccga
    14041 atataaaaaa tggcaagcaa ctgctggcca gtctgctaca gaatctgaaa aattagcaaa
    14101 agcacaagaa tatgtcggca aacagagtga tattgctgaa caaaaaatcg aagtgctaaa
    14161 aaaacaatta gatgccacac aaaaagaatt tggagaaaca tctaccgaag ctttagaaat
    14221 gaaaactaag cttaatgatg cggaaagaga attcgaagaa ttaagtaatg ctgctaaaaa
    14281 cgttgatacc tccacagtag atgatatcgg caaaaaactt gatatgggta atttaatgga
    14341 agctactgat cacttgtcag tgatcggaga taagcttatt gatgtaggta gtaagtctat
    14401 tgaagcagct ggaaaagcac aagctatgca ggcccaattt aaacaagtct ttggctcttt
    14461 agaaggggaa gcacaggacg ccgttgaggg aatggctgaa gaatttggaa tgttaccaaa
    14521 tacgatcaag cctgttttta cacaatatac gtcaatgttt aaaggacttg gatatgatac
    14581 caaagaagct atggagttag ctggtgatag tactcagtta gcagcagatg cagcagcttt
    14641 ttacgataag tctatggatg atgctagtga atctcttaat tcatttataa aagggaacta
    14701 cgaaggtggg gagcaaatag gtttatttgc taatgatact caaatggcag cttatgctgt
    14761 taagcataat ttgataccag cgactgaagg agcaaaaaaa gccagtgaag agtcattgtt
    14821 agctgttgaa aaagcacaat ctaagtatgc tgatgctatt aagaaacatg gtgaaggatc
    14881 tttagaagca agagaggctg ctttaaaact taaagatgcg caagataaaa taaatgaaga
    14941 attaggccca caaacgcaaa aatggtctga tttagatgag gctaccaagc aagcagttcg
    15001 agttcaatat gctgaagata tgcaaaaatt agccggtgct acaggtcagg ctagtaggga
    15061 gtctgatggt ttagaaaacc aaatgacaag agcaaagcaa gcgttagaag atttttatgc
    15121 ctcattgggt gaagatatac tgcctgtgtt tatcaaaggg ttgcaagcag gagcgaaagc
    15181 tttgcaagga ttagccgaat ggtggagtaa acttgatggg ccaatgaaaa atttcatttt
    15241 agctcttgga ggaattctag cattattaag cacattagct cctgttataa ccgcagttgt
    15301 tacgatagtt ggcacatttg gttctacagt tttgctgcca ataataggaa ttatagcggg
    15361 cgttgcagct gtaatagcga ttgtaattac agcgtttcaa aactggggcg caatcactga
    15421 ctggtttagt gatttatgga aaaaatttac cgattggtta ggtgatacct gggaaagtat
    15481 aaaagaaagt gcctcatcag tttgggatgg agtcaaagaa acctggtctg gatttgtaga
    15541 ttgggttcaa gaaatttggc aaggagtttc tgattggttt ggagagttat ggagcggatt
    15601 agttgaagga gcttccaaca tctggcaagg agtccaagag acttggcaag cattcgttga
    15661 ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat
    15721 tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa
    15781 tattatcgtg ggtatttttg atgtagctgg acagttatta ggcgcagctt ggcaagctgt
    15841 ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag
    15901 catcgcttgg aatggaattg taactacatt ccatactata tttgatccag tagtggcatg
    15961 gtggaatggt atatggacat ctattagtac tacggcttca aatatttgga actcaattag
    16021 tgcaacagct tctagtattt ggaatagtat caagaataca atcactagct tggtacaagc
    16081 agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg
    16141 gaattcaatc agctctacag catcaaatat ctggagtagc gtgactagca gtataagcaa
    16201 tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat
    16261 cagcggaatt tggaacggta tcaaaaacac tgctttgaat ctttggaatg gaattacaag
    16321 cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct
    16381 aacaggtatt gtttccgaca tattcaataa tgttaaaagc acgatagcta atatatggga
    16441 aggtatcaaa aagactgtta gcgctccgat tgattggatt agagataaaa tcagtagtgt
    16501 ctttgataat ttgaatattt cgataccaca tattccgtta ccacatttta aattgagcgg
    16561 agaattcaat ccattgaagg ggaaaatccc aacattgggt gttgattggt atgcgaaagg
    16621 tagtgtgttt aattctccaa atattatcgg tgtcggtgaa gcaggacctg aagcagtttt
    16681 acctttgaaa agatctgtgc tgcaagaaat tggggatcgt atcttgagta gcacatcagt
    16741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    16801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    16861 aaaagttcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    16921 ttttagtttt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    16981 tttcctgatt atttgcataa attggcttta gcggaaagac caaccatacc ttctccaaaa
    17041 agacagagag ttgaaacttc tgttttagga cggctaggtg gcttagtaca agattactcg
    17101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    17161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    17221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    17281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    17341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    17401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    17461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    17521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    17581 aacaaaatga agaccatgca atatccgaca ttgcaagtgg atatcaatca ttttaattgc
    17641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    17701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    17761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    17821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    17881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    17941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    18001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    18061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    18121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    18181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    18241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    18301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    18361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    18421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    18481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    18541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    18601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    18661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    18721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    18781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaacggt
    18841 ggcgaaatca gtgatttact caatgatatt gtagatcatc aaactgatat gattactggt
    18901 caaaatggtg gttatgtttt attagatcct aaagaagcgc ctagtcgtat tttgattatg
    18961 gacacaccag ataagaatac cgcaaggaat gttttacaaa tcaacaatgc tggtattggt
    19021 ttttctaaaa ctggcattaa tggaacatat gaaacggcat ggacgttaga tggcggattc
    19081 aatgcctcgt ttattacggc tggtgaaata gtaggaatta ctattagagg tactacatta
    19141 attagtgatg gtgctgatta tagaacaagt attgctaatg gcaaaatgac ttggtactca
    19201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    19261 ggtattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    19321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    19381 ccaaatttca gatatagcaa tgctagttat gtaacttctg gcgatggtaa ttctttgggt
    19441 atagatggta gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac tatgaaattt
    19501 actagtgatg gtatgctaac gttaccaggt tgtttttttg gttcatggga agatgggaaa
    19561 cttgctaggt ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    19621 aaaaactcaa ctgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    19681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgacgatgg taaagttcgt
    19741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    19801 acacctacag aactagtttt gtgtgctgtt actcatgaag atattgacca tttcatcatt
    19861 gaaactagta agccaaacgt attagttaga tggaatttag tggcacaccg tctagggtat
    19921 gaagatatta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    19981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    20041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    20101 ttacggtgac tgttaaacca tttttaaatg gcttgcagat ggatacgagt ggcggaacat
    20161 ttactttaaa aggaacaaca ccatctaacc gttatgtaga tagtgttgca actagcgtaa
    20221 ctagtgaaga agtcatattt tctcttgatg gcacatttat gagtgaagca ggatattata
    20281 aacactgcta cgtagaatat agaaaaggta atcaaatttt aacaacgcaa gacatcattt
    20341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatac gtttcgcaat
    20401 tggaagagtt gattcgaaag tataacgaaa cttttgatgc ttttatggct gaaattaaag
    20461 gtagagtgga tagcttaaat caacagatta ctgatttaac tggtcaagct aaaacgctac
    20521 aagacaagtt agatgctctg aaagaagaaa tttctaagtt aggtaactta caagtgatgt
    20581 acagcaacag catcgacttc ggtgactatg attattcggg gaacccgaat ttgttaagta
    20641 agctatcata cgacttaatt gaaaatcaaa atacttcagc tggaacactt tctaagggtg
    20701 aaaactcgtt taaatataat aagatatcag ctgaagtgga aggtggagta gagttatatt
    20761 ataaacgaag aggtatagct aactggttac cctctaataa aacgcttgta atgactgtta
    20821 agcttagagc tggagcggac tatagtccag ttgacggaaa acttatactg attagatata
    20881 ggtatgttgc cagtggaact ggcaagattg ttttagactt acctattaac agtaattcga
    20941 taactcagga atggaaagag tttagtatta ctggaactac tccaacattt agcccacaag
    21001 cataccatcc ttggatacaa tttagggctc aagatggggt acttggggaa atagaaatga
    21061 gctatgacat caaaatcgaa gaaggctcaa cagctacacc ataccagcct aatttgctgg
    21121 acgctccaca ttatttaagt aaaacaccat taggtgaaaa tattgctaat aagtctgtga
    21181 ctttcccaat taacactagc gcgtataaac tatatgaata taatacggaa gaagatctta
    21241 taataggtca aacgtatact attacgctta aaggaacaaa acctgcaagt caaacctttg
    21301 cagtgtataa ttattggaac attaatcttg gaagcttaaa accagttgag ggattgacag
    21361 acgtatggtc tataacattc acaccaacga cacttgagcc aggtttgcct aaagaccttc
    21421 gcattcttca gtcgcctaaa gaaacggccg gcgcatgcca aattgactgg ctcaagattg
    21481 aaaaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga atcggcatgc
    21541 gagactcaaa taatccaaaa gactatgtct gggatttagc accagaatat gtagaaaaca
    21601 atctggctac agatatcaaa gttgctgaaa ttactggtaa agcaaacaat tataccgatg
    21661 ggaaagtatc gcagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag
    21721 ctaagggtgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata
    21781 agatcgatga acgcattaat gatacagcta ctaccgcatt ttatggcgca caaggtgaag
    21841 tctctaacaa aacgaaagta gcggagtatg gagtaggaac agggtatact actacagctg
    21901 taggagaaac acatttcgaa cgtcaatcta acggatacgt taaatgctta aaagctggta
    21961 agtattcaat tagtgcgcaa atacgtgtgc aattgggcag gaaatatgct tcatggttat
    22021 atactgattt atataaagat ggaacttctg ttgataattt agtatcttat ggggtcgacg
    22081 ccttgcaaaa tagatttgca gctagcggaa acattgtaac tgatttagaa gtaaacgatg
    22141 tactaaatat gcgtactgaa atcggcgttg cagatggaat tttacaattt acatcttgcc
    22201 gtactttagt cttgactaga ctatctgatt aatacaatat ttataatttt gctatcaaca
    22261 cgctcaaagg agggtgtttt ttattttgca atgaaaggag gctagttggt tgaaagacga
    22321 agcaatacaa gacgtggtag aacgcttagt gcgtattgaa acgaaactgg ataattacga
    22381 attattacgt gaaaaagcgg aaagtgcaaa agatagagcg gatcaggcat attctattgc
    22441 gcttaataat gcagaagaca tcaaagagat gaaagccaat aataaatggt cgtggggtta
    22501 catgatcggt ttaggcatta caatcattgg ctatttcttg actaaattgt aaaggaggtg
    22561 agaagaaatg gttttaccag ataagtatta tcaagtcatt aaatggacag ttttaacagt
    22621 attgccagct gcatctgtat tagtagcgac gttagggaaa gcgtatggat ggaatggaac
    22681 agatatgaca gtactcacta tcaatgcagt agcaacattt ttaggtgtta tcactggtgt
    22741 gtcggcttat aatttgaaaa aataggagga aacaaatgaa aaagaaaatt actattactg
    22801 cgatgagcct attaacggct ctttttttat tgccaattaa tgggtttgcc tatactatca
    22861 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtaca
    22921 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
    22981 aacgttcatg gactagtgct tacactgctt acattgtggg tgatggcgga attgtttacc
    23041 aagttggaca acctggttat gtacagtacg gtgctggttc gtatgctaat gccaacagtc
    23101 ctgtgcagat tgagttacaa cacacacata ataaagcaac ttttgaaaag aactataaag
    23161 catacgttga attggcgaga gattccgcta agaaatacgg tattccgctt acattggaca
    23221 cgccttataa ccaaccagga atcaaatcac atttatgggt aacacaaaat atttggggcg
    23281 atcatacaga tccttacggt tatctttctg aaatgggcgt aagtaaagaa aaactagcct
    23341 atgatttagc tcatggattt accgatgaaa atccgacaac ttcagatgat aaaccagtca
    23401 ttgatccaac tagagcaggt gcagcaaatc ctacgctgac agatggaaca aattacgccc
    23461 acattgatca gttcggagaa atcgaaaacg caaacttgca tgtggctgga tggcacattg
    23521 ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa gaattagctc
    23581 gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat acttctggaa
    23641 atgttggtta tcatgtatct ttcaatatgc gtaattttcc taataagaaa gtctatgtca
    23701 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
    23761 acaaacgttg gtatttgaat attccgcaac gataaaaaat ggcccctcgt tgtgatgtgc
    23821 accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg gaaatataca
    23881 tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg ctacaaatat
    23941 cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag gatttataac
    24001 tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa atttaaactt
    24061 gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc taaaaaattt
    24121 aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga aggcggtgct
    24181 tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa gctaaacatt
    24241 cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag agcagagaac
    24301 gcatat
//
LOCUS       DAXWLH010000030        23576 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXWLH010000030 DAXWLH010000000
VERSION     DAXWLH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.030"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            21..1448
                     /locus_tag="ACGE36_000866"
     CDS             21..1448
                     /locus_tag="ACGE36_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383284.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="ACGE36_000867"
     CDS             complement(1540..1905)
                     /locus_tag="ACGE36_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8383285.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="ACGE36_000868"
     CDS             complement(1902..2267)
                     /locus_tag="ACGE36_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8383286.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="ACGE36_000869"
     CDS             complement(2459..2707)
                     /locus_tag="ACGE36_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383287.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="ACGE36_000870"
     CDS             2706..2834
                     /locus_tag="ACGE36_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFF8383288.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="ACGE36_000871"
     CDS             2891..4585
                     /locus_tag="ACGE36_000871"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFF8383289.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4685..4981
                     /locus_tag="ACGE36_000872"
     CDS             4685..4981
                     /locus_tag="ACGE36_000872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383290.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5359..6492
                     /locus_tag="ACGE36_000873"
     CDS             5359..6492
                     /locus_tag="ACGE36_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFF8383291.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6523..6843
                     /locus_tag="ACGE36_000874"
     CDS             6523..6843
                     /locus_tag="ACGE36_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFF8383292.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6872..7336)
                     /locus_tag="ACGE36_000875"
     CDS             complement(6872..7336)
                     /locus_tag="ACGE36_000875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383293.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7467..8720)
                     /locus_tag="ACGE36_000876"
     CDS             complement(7467..8720)
                     /locus_tag="ACGE36_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8383294.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8749..9066)
                     /locus_tag="ACGE36_000877"
     CDS             complement(8749..9066)
                     /locus_tag="ACGE36_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8383295.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9092..9412)
                     /locus_tag="ACGE36_000878"
     CDS             complement(9092..9412)
                     /locus_tag="ACGE36_000878"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8383296.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9876..11291)
                     /locus_tag="ACGE36_000879"
     CDS             complement(9876..11291)
                     /locus_tag="ACGE36_000879"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8383297.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11308..12624)
                     /locus_tag="ACGE36_000880"
     CDS             complement(11308..12624)
                     /locus_tag="ACGE36_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8383298.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12774..13472)
                     /locus_tag="ACGE36_000881"
     CDS             complement(12774..13472)
                     /locus_tag="ACGE36_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFF8383299.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13761..14754)
                     /locus_tag="ACGE36_000882"
                     /pseudo
     CDS             complement(13761..14754)
                     /locus_tag="ACGE36_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14835..15668)
                     /gene="msrA"
                     /locus_tag="ACGE36_000883"
     CDS             complement(14835..15668)
                     /gene="msrA"
                     /locus_tag="ACGE36_000883"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8383300.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDTDYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15902..16645)
                     /gene="truA"
                     /locus_tag="ACGE36_000884"
     CDS             complement(15902..16645)
                     /gene="truA"
                     /locus_tag="ACGE36_000884"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8383301.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16836..17471)
                     /locus_tag="ACGE36_000885"
     CDS             complement(16836..17471)
                     /locus_tag="ACGE36_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8383302.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17618..18838)
                     /gene="sstT"
                     /locus_tag="ACGE36_000886"
     CDS             complement(17618..18838)
                     /gene="sstT"
                     /locus_tag="ACGE36_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFF8383303.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(19180..19857)
                     /locus_tag="ACGE36_000887"
     CDS             complement(19180..19857)
                     /locus_tag="ACGE36_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFF8383304.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20108..20650)
                     /locus_tag="ACGE36_000888"
     CDS             complement(20108..20650)
                     /locus_tag="ACGE36_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFF8383305.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20655..20882)
                     /locus_tag="ACGE36_000889"
     CDS             complement(20655..20882)
                     /locus_tag="ACGE36_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFF8383306.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(20961..22841)
                     /locus_tag="ACGE36_000890"
     CDS             complement(20961..22841)
                     /locus_tag="ACGE36_000890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8383307.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23142..23462
                     /locus_tag="ACGE36_000891"
     CDS             23142..23462
                     /locus_tag="ACGE36_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8383308.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECFFIYDI
                     IASK"
BASE COUNT         7309 a         4835 c         3984 g         7448 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
     4741 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
     4801 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
     4861 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
     4921 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
     4981 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
     5041 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
     5101 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
     5161 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
     5221 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
     5281 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
     5341 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
     5401 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
     5461 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
     5521 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
     5581 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
     5641 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
     5701 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
     5761 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
     5821 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
     5881 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
     5941 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
     6001 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
     6061 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
     6121 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
     6181 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
     6241 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
     6301 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
     6361 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
     6421 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
     6481 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
     6541 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
     6601 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
     6661 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
     6721 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
     6781 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
     6841 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
     6901 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
     6961 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
     7021 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
     7081 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
     7141 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
     7201 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
     7261 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
     7321 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
     7381 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
     7441 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
     7501 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
     7561 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
     7621 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
     7681 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
     7741 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
     7801 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
     7861 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
     7921 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
     7981 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
     8041 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
     8101 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
     8161 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
     8221 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
     8281 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
     8341 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
     8401 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
     8461 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
     8521 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
     8581 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
     8641 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
     8701 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
     8761 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
     8821 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
     8881 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
     8941 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
     9001 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
     9061 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
     9121 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
     9181 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
     9241 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
     9301 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
     9361 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
     9421 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
     9481 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
     9541 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
     9601 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
     9661 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
     9721 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
     9781 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
     9841 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
     9901 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
     9961 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    10021 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    10081 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    10141 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    10201 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    10261 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    10321 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    10381 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    10441 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    10501 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    10561 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    10621 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    10681 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    10741 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    10801 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    10861 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    10921 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    10981 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    11041 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    11101 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    11161 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    11221 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    11281 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    11341 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    11401 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    11461 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    11521 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    11581 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    11641 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    11701 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    11761 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    11821 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    11881 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    11941 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    12001 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    12061 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    12121 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    12181 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    12241 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    12301 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    12361 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    12421 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    12481 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    12541 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    12601 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    12661 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    12721 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    12781 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    12841 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    12901 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    12961 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    13021 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    13081 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    13141 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    13201 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    13261 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    13321 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    13381 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    13441 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    13501 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    13561 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    13621 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    13681 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    13741 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    13801 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    13861 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    13921 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    13981 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    14041 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    14101 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    14161 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    14221 atttttttgt atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc
    14281 ttacaagttg catcaacgct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat
    14341 gatgatagaa gatgtcaaca taatctagcc ccattctttg gaaactttgg tcacaacttg
    14401 atatgatgga tttacgagag ccccattccc catatggccc ttcccacata tgataacctg
    14461 ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc
    14521 caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg
    14581 taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat
    14641 caccaaaatt ttgccataat ccgagaggag agataaaatc ggtaatttta aacctgaatg
    14701 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    14761 tcctcctcat caaatctttt taaaacgctt tcttgttttc tatttttagt ttactacatt
    14821 ttaaagaaaa ggagttaatc aaaatgattt tttcgtatat ttttttgcca tttgtttttt
    14881 actcgttgat agatcagaaa ttgattacgt gcttgctgga tttttttata gcgtttcggt
    14941 tgttttttgt aaaattgctg atgatagttt tctgctggcc aaaacgtacc ggctggttcg
    15001 atttttgtga cgattggttg gtggaaagtt cctgaactgt ctagtttctg ttttgattgt
    15061 tccgctaatt ctttttgtcg ttcgtcttga taaaaaatca ctggacgata ttgcttccct
    15121 ctatcttgaa attgtccaaa tgtatctgtg ggatcagtaa tttgccaata gattgtcagc
    15181 aactcgctat aacttatttc tcttgtgtca taaatgatct ccacagcttc cacatgacct
    15241 gtgtaaccac cacttacttg atcataattc ggtttttcaa catgtccgcc cgtgtatcct
    15301 gacaaaacag ataggatccc ttttttcgat tcaaacggct cgaccataca ccagaaacat
    15361 ccccctgcaa atactgcacg ttcttgatag tcagtatctt gcatggcatg cttggaaaag
    15421 tcatattttt gctccttatc aaatccttcc gtaatcaata aataaaagtc cattacatca
    15481 ggcgtcagat tcattcggat agctaatgga cgtagtgtta cttctagttt tgataactgt
    15541 tctttaacgg atatattttc tcttgtgtca ttctttgtct gaatcaatac ttttcgttcc
    15601 caatctcgtg tattcggatt caaaatcaga ttataaagtg tttgaatgaa tatttgttcc
    15661 tcatccatag attctcctcc tttccgaaga agacaagcta gtgactttag ctgtaattta
    15721 tcataaagtg aggaaataat cgattgaatc gtatttattc aaagaagata agcagcttca
    15781 gatgatataa gatgatcttt atcttaaaaa gcatactcta tggtttggtt taactaaaaa
    15841 acgacctctg aaaaaaggga tgttctccaa ttctcagaag tcgagtaaag atgattcttt
    15901 tttatgagcg ataataaact tcatccaaaa acagaccttg tgcagggacg gtttctcctg
    15961 catgttgccg cactttgctt tcaaatattt catcgattgt ttctatcggc atggcacctg
    16021 tgccgatttc tagtaatgtc cccatgataa tccgcaccat tttatataag aatccgtctc
    16081 cgacaaaagt aaaatgaagc atttctccct ttcgttcgat agataattca tcaatcgtac
    16141 gcactgtgga tttttttgat tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca
    16201 ctaatttttc gcaagctgca ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc
    16261 tgtggtttcg ctcaaatgct gaaggaatca cgttgttcca aacataatag ctgtattttt
    16321 tgcccaccgc attatatctt gaatggaagc gctcaggcat ttcttcgatt cttttgatga
    16381 caatatcccg cggaaggtaa cgattcataa agtcgatcat ttctgccagt ccaatttctg
    16441 tgtttgtttt aaaatttgct acttgtcctc gcgcatgggt gccagcatct gttcttcctg
    16501 atccaataat ttctatctct tcatttgtca tctgatgcaa tactttttcg atttttcctt
    16561 gaatggtttt atcgtcatct ccaagtcttt gccatcctga atatcttttt ccgtcatatt
    16621 cgattgtcaa cttgatgttt ctcatatttt agtggctcct ctgtcttcca tttggtcttc
    16681 tctctacctt tttaagattg tagcacaaaa tatagctcct tgcttctcta agacagaagc
    16741 tatacaagaa aactgtggaa atatatattt cagaaaaaca aaaaacagcc accggatgaa
    16801 gtatccgctg actgttcaat ttgacggctc aagttttact gtttgagatg caagatactc
    16861 aaccaggcaa tgggcacata attaaagaaa aaactgattc tttggcaaaa acctatttta
    16921 ttcagtaatg gccaagaatt tatagaagga attcttgcca gtccataaat cgccgcaaaa
    16981 agcaaactga tcacagacag tccaaggtat aagtggctta cgtccatttg cccatttttt
    17041 cgatagatca agaaaagaag aactggaaac aataaaaaac cactgtaacc taaacctgat
    17101 ccgatattat ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac
    17161 aaacctgtaa agatacaatc acctatacca tagaacgcga tggctgctgt aactagggta
    17221 gctaacacac gagatgtact tacaaatgca gcatacaatg caggcaatga caggatgaaa
    17281 aagatccctg aaaccacaga ccaaataaga aacgcttttc ttacaggact ggaaacctct
    17341 ccaaatacac tgatcacgct cttcatttga tctaattcag gataatacaa tcctaatacg
    17401 tacggagtca aaaaatcact cagcacgcct aataataaaa agaaaaaacc aaatttacgt
    17461 aaaaaatcca tgatacgcct ccaataacaa ttacctatgc ttttaattgt tattataaac
    17521 gactttatct tgatttaaca gaaacctcta tttacacgaa catctatata actcattaaa
    17581 aactcgaatc attttaaaaa tactaaatat ctaacgttca acgttcactt aatccatcta
    17641 aacaatcttt caatttgatt ttttccccac ttcttttacg cgcgcctagc tctcctattg
    17701 cagtaaacaa cagatcactt gacgaattta aggcagtttc cattgaatcc tggataacac
    17761 cgataataaa tccaatacca acgatttgca tcgcgacatc attagagata ccaaacaaac
    17821 tacatgctaa tggaatcaat aacagtgaac cgcccgcaac acctgaagct ccacaagcag
    17881 ctaaagcaga caaaatacat aaaaggactt tcaaaacaaa tggtgcttca atccctaaag
    17941 aatgtacagc agtcaacgtc atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg
    18001 tagcacctaa cgggatcgat atggaataag actcttttgt caatttcaat ctttcagcca
    18061 acatcatgtt tatcgggata tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc
    18121 tttctttcaa acaaaagaaa atcaatggat agggattttc tttaataaac aaaaacgtga
    18181 ggaatgggta cacaactaat gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct
    18241 gcagatactc tgccaatcca gcgattccag tcgtagcaat tgattgataa accaatccta
    18301 aaatccctat cggagcaaat gcaataatca cttgtacgac ttgtgacaac gctatagata
    18361 tctgatccac caactctttt gtcggttctg aacgtgctcg aaaagctagt cccaacaata
    18421 cagcccagaa taaaacgcca aggtaattcg catcaataag agattgaaca ggattttgtg
    18481 tgacattcgt caacatagta gataaaacac tttccaaact ttgcggtgct ttttcagata
    18541 ctgtttcttg taaagtaata ggaacagtaa aaattgaact tacaatgact gctacaagtg
    18601 cagaaagcaa tgttcccact aaatataaga tcaaaatagg cttcacgaat gtcttattcc
    18661 caattttatg cttggcaatt gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg
    18721 ccttcaagca actgacaaac aacgtcccca atattgaaat aaatgtccac gtggggaaaa
    18781 agataccaaa caatattccg actaaaatac caacaacaat acgttgaact aacgacatat
    18841 ccaaaaactt ctctaacaaa tgaccaccct ttcacaattt taacaatcat tgtatcattt
    18901 tatctaaaga aactaaaggt gcaattttct aaaaattcta ataatacaat tggcaattca
    18961 tgctcttgca acattcaatt ttagtattgg agggtttatt caatctctaa agaagtcgag
    19021 agaaaactat ttatcgttat tattatagtt atccaagtaa aacgtaacta agatgaagga
    19081 cgaaactaga aatttcatcc ttcatcttaa ttcttagaca tagcaatcaa ttaatatttt
    19141 ccactgagtc tcttctttct ctcactctca ctggtttact tacccataaa cgatttgatt
    19201 aaagccattt ttcaattcat tattcgctgt ctcgttaaag taaagataat tatcgggatt
    19261 cccctgattt tgttgtgtca ccaatacttt tggctgattt tgctgaaacc cgaatagata
    19321 gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa tagacggctg tgatttgata
    19381 gtctgcttga cctgtcccac tttctgacca ttctgcagaa acaggcactc caccaatagc
    19441 catcttccac tccccgttca aaataacttg agggacttta agaccatata agtccacact
    19501 catttgattc gtatagcttt tgtattgttg tcccattgtt tggccccatg tagccataaa
    19561 tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt tgttcgactt gttcgctgct
    19621 ttctgaagat gaagcctcta cagaagaact tgaagtagat gtttgagttg tttcagtcgt
    19681 tttggaagac tctgacgaag tagaggtatc tgctgtttcc gtacttgctg cagttggctt
    19741 gctctctgtg ctagttgttg tagtagttgc aattgttgct ttcgctgttt cttttgttga
    19801 ttcttttttc gattcattcc cgttggtaca accagctaac aaaaaagcta gaccaattgt
    19861 tactaaaatc ctttttttaa attaaaaaac tcctttcgat tactcccact tctttattct
    19921 atcaaataag caatgtgatt ttattgattt tttgtatttt ctcatccatc ttttaaacct
    19981 tttaaaaact aagaagcacc cgctgatgtt gtctgtaata catcagcggg tgcttctttt
    20041 attttaatct tcgcgatttt ccaatttacc tcacgcttta tatttgtatt tgaagaaaca
    20101 tagactttta gtcatcatcg tcttcctcat ccaaatcttg tcggacagtt tttccttgga
    20161 gtctttgtac ttggtagatg atttctttat tgaaacttaa aagttcaaca agcaattgtt
    20221 tcaagccata tttttcttct ttttcagcta atttgattgc ccgtgtgata aagacatttt
    20281 ggaaatcaaa atcttttgca aagttttcca acattgttga ggctggtaaa tatttgtttt
    20341 gaccatgttc gtaaattttt ccataacgca tgacttcttc taaagtagac ggaacgattt
    20401 cattttcaat cactaactca tgagagattt tattgaacca tctcgcttct tgatcagcat
    20461 attctgaaaa aagggtactt aattcttgcg aagatgtacc caaaacatac aattgtgctt
    20521 ggatcaattt catttcttct aatttaaaat ttgccaatac atgccccacc atggcgccag
    20581 cagttggttt atgatgatcg atatctgctt gttttgtttc ttgagcaaat ttttcttcca
    20641 ctatattcac tgatttacgc ctcttttact tttgttgatt ggacttcata acctaatttt
    20701 tccacaactg cagctaattg ttcagggtgg acttcatctg gatcaaagtt tgctttgatt
    20761 ttgctcgcat taaacaaaac tttcgtattg cttaccccag gctgttgaga aaccgcatgt
    20821 tctatttttt gcatacagct tggacaagct agtgtatcta attgcataat catttttttc
    20881 atcgtagttc ttccttcctt tttcttgata gtcttattct agattacagc ttccataaaa
    20941 aaattgattc ttatcaagtt ttacgatttt tcttctttgc tttcgtgtcc aaaatacaat
    21001 aaacgcatcg cgttaaagat aacgatcaag atactagctt cgtgaacgaa catcccacta
    21061 gccatgtaaa taaacccggc aaataagccg atgagtaaaa ataagacgac accaatggca
    21121 atcactacat tttcacgggt attcaaaaca gttttcttag ccaaacggta agcatgaacc
    21181 aacgatccaa aactagattg catcaagaca acgtcactcg tttcgatagc gacatccgtt
    21241 cctgatccca tagcaatccc gatatcagcc gcagccaaag agggagaatc attgattcca
    21301 tctccaatga aagcaacgtg caatccttgt tcttggtatt ttttcacaaa ttgaaccttt
    21361 tcatctggca ataattcagc atggacttca tcaatgccta acatgtcagc tacataatca
    21421 gcagtcatct gattatcacc tgtcagcatc actaaatgtt ttgctcctgc ttgtttcaac
    21481 atagctaatt gttcagcgac ttctggtcga atcacatctg acacaccaat gatttgagtg
    21541 atttttttgt cgattgctac aataatgact gagctgcctc tttgttgtaa ttgatgtaaa
    21601 tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg caatcaattt ggtgttgccc
    21661 gcacaaactg tatgaccatc aatctcagct gtgattcctt gtcccttgat cgtctgatta
    21721 tctttgactg ctaattgttg aaaatcaatg tctttctcct gagcataatg gatcacggcg
    21781 cgccccagag gatgatcgga taacgtttct aatcgagcca ctaaagctaa gctgtcccct
    21841 tcatcctctg tatagttctt gatagtggat acagcagtag ttcctttggt caatgtaccg
    21901 gttttatcaa agacaaaagt atcgattttg gaaaaagtat ccattgcttc accgcctttg
    21961 actaagatgc cccgtttcgc tccattaccg attcctgcta cattcgaaac aggcgcacca
    22021 atgactaaag cgccaggaca accaagaacc aaaacagtga tggctaaacg aaaatcacga
    22081 ctgatcagac caacaagcaa cgcaataagt aaaacggttg gagtataata ctgagaaaat
    22141 tgatcaatga atttttccgc atgtgatttg gtatcttgtg cttcttcgac taattctata
    22201 attttggcaa aggtcgtatc atctccgacc ttagttgctt cgatcgtcac gtagccatct
    22261 gctaaaatcg ttccagaaaa tacgttatct ccactgattt tgacgacttg tcgtgattca
    22321 cccgtcactg cggcttcatc cgcataccct ttcccgtcaa tgactcgtcc gtccacagga
    22381 atagctccac cagtttttac taagacgata tcgccttctt ccacgtcatc gatttccact
    22441 tcttcggtcg ttccatcttc ttggacaacg ctggcagtcg atggggccat ctcggtcaaa
    22501 gccttgattg attcccgcgt tttggtcaat gttttttgtt ccagataact accaaacaaa
    22561 aacaagaatg taacgatagc tgactccttg tattctccga tcataaaggc accaagcaca
    22621 gcaatagtaa cgagtaaatc aattgagata actttgactt ttactgcctg atacgcatgg
    22681 attataatag gtatgaccgc aataatagaa gctatggcta gagcaatgtt atagaacaag
    22741 acaaatccag taaatttccc gataaaacca aaaataatca acacagctga taaggcggtg
    22801 atatgatttc gatgctcttg gatccacttt tgtaatttca tctttctccc tcttttcctt
    22861 ttcattctta cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa
    22921 gtttattcaa aaaggcggtt ttcttttggt atactgaatt cctacagtca ttaaacgatc
    22981 atatagaaaa aggaagcgcc gaaacgcttc cttaggtagt tctatttttt tattcaaaga
    23041 taagatgctt tgtcatctta cgactgaaga acaacaatca aaaaagggca gcagccactc
    23101 attcacaaga aatatcgttt tttgatatgg gagttgaagc agtggacaaa tatctctact
    23161 tattagctgg gaataagatc caaaaaagct taatggattt tatccaagaa ttggaatgta
    23221 ctttccacaa aaaattcact cattccattc ttttaaaact tttgattcat acagcgtgtc
    23281 taattgagcg tacattgata aacggtcatg aacttaaaat tatatcagaa gatgacacaa
    23341 gaccttctca tgaaacaatt tttcatgtaa aaaaggcctt caaaaatatt gaaacagaat
    23401 ttgggatcac tgtttcttat gatgaatgtt tctttatcta tgatattatc gcatctaaat
    23461 aatctttttt tatgtaaacg agtattttgg gagcgtcaat aattttgtgt aaataaattg
    23521 tcctcctgca aaataattag ttactcagta aacattgaaa ctaatgtatc ggttac
//
LOCUS       DAXWLH010000031        23066 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXWLH010000031 DAXWLH010000000
VERSION     DAXWLH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.031"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            90..2531
                     /locus_tag="ACGE36_000892"
     CDS             90..2531
                     /locus_tag="ACGE36_000892"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFF8383309.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2861..3565)
                     /locus_tag="ACGE36_000893"
     CDS             complement(2861..3565)
                     /locus_tag="ACGE36_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383310.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3752..3997)
                     /locus_tag="ACGE36_000894"
     CDS             complement(3752..3997)
                     /locus_tag="ACGE36_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383311.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4014..4865)
                     /locus_tag="ACGE36_000895"
     CDS             complement(4014..4865)
                     /locus_tag="ACGE36_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8383312.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5031..6035
                     /locus_tag="ACGE36_000896"
     CDS             5031..6035
                     /locus_tag="ACGE36_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFF8383313.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6276..6632)
                     /locus_tag="ACGE36_000897"
     CDS             complement(6276..6632)
                     /locus_tag="ACGE36_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8383314.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7000..8187
                     /gene="tuf"
                     /locus_tag="ACGE36_000898"
     CDS             7000..8187
                     /gene="tuf"
                     /locus_tag="ACGE36_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8383315.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8375..10966)
                     /gene="mprF"
                     /locus_tag="ACGE36_000899"
     CDS             complement(8375..10966)
                     /gene="mprF"
                     /locus_tag="ACGE36_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8383316.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10992..11753)
                     /locus_tag="ACGE36_000900"
     CDS             complement(10992..11753)
                     /locus_tag="ACGE36_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8383317.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11920..12552
                     /locus_tag="ACGE36_000901"
                     /pseudo
     CDS             11920..12552
                     /locus_tag="ACGE36_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            12611..13471
                     /locus_tag="ACGE36_000902"
     CDS             12611..13471
                     /locus_tag="ACGE36_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFF8383318.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13656..15971
                     /locus_tag="ACGE36_000903"
     CDS             13656..15971
                     /locus_tag="ACGE36_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8383319.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16153..17994
                     /gene="dnaG"
                     /locus_tag="ACGE36_000904"
     CDS             16153..17994
                     /gene="dnaG"
                     /locus_tag="ACGE36_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFF8383320.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18018..19127
                     /gene="rpoD"
                     /locus_tag="ACGE36_000905"
     CDS             18018..19127
                     /gene="rpoD"
                     /locus_tag="ACGE36_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFF8383321.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19347..21446
                     /locus_tag="ACGE36_000906"
     CDS             19347..21446
                     /locus_tag="ACGE36_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFF8383322.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21642..22505
                     /locus_tag="ACGE36_000907"
     CDS             21642..22505
                     /locus_tag="ACGE36_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFF8383323.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22610..>23066
                     /locus_tag="ACGE36_000908"
     CDS             22610..>23066
                     /locus_tag="ACGE36_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8383324.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         7769 a         4038 c         4807 g         6452 t
ORIGIN      
        1 taaaatttaa tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga
       61 atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa
      121 gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca
      181 cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag
      241 taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt
      301 tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg
      361 gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga
      421 tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa
      481 caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt
      541 caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag
      601 aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg
      661 gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg
      721 atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct
      781 cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg
      841 tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca
      901 aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca
      961 ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca
     1021 atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa
     1081 aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac
     1141 gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt
     1201 cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa
     1261 cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc
     1321 aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac
     1381 tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa
     1441 tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag
     1501 aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg
     1561 cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc
     1621 aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa
     1681 gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca
     1741 tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg
     1801 gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc
     1861 ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg
     1921 cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg
     1981 ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc
     2041 acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac
     2101 gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa
     2161 cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg
     2221 ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag
     2281 atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc
     2341 tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag
     2401 ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa
     2461 aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc
     2521 gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt
     2581 tactgcagat cttgcttttt tctctatcag aaaatatgtt tattattaga attttttata
     2641 gttaaggtta ggcatcttaa gaaatcaggc aactccaaga tatcttcagg tagaagagaa
     2701 gtcgcctaac attgtaggat cataggtgat ttgttggaca ggtcaagctg atatttgagc
     2761 ctgatgttag aaggaatttc tttttacttg ctgtagaaat tattatatga ctaaaaaggc
     2821 tgggatttta aatcccagcc ttttatgaca aatagaattt ttatcgatca gtaatcgctg
     2881 tcgctgcaaa ttgttcgatc aaaggaatca aagtcaattt gactttgtat ttccgttcga
     2941 gtgctttttc gataacgaac tcattttcag tagtcataga aatagttgct aatttacgag
     3001 gttcatcagc atttttttca ttaataacta cttcatgttc attttgttct gtgtaaaatt
     3061 taggatactc agcaacggct tttgaaaaat cagcaaaact taaagcggca ttcgtatcag
     3121 ttgagcgtgt attttcagtt gtgtccgctt ttggtttttc cgttttttct tctggcacac
     3181 tgactgtttc ttctgtaact ggtgtcgcaa cagcttgcgc agttggttca tcgtttttca
     3241 atcgttgatc attccagtaa gacacgattt caataagaag attgtcacgg aatcgttcga
     3301 aaatcccgtt ttctcctgaa cgaattctct ttaatacagt attgaagttg tcatcgattg
     3361 gcacgttgat ccgtgtatct tcatttgttt tcccagctaa tccgatcgtt aagatttgac
     3421 ggtttttggg atcaagcatg acttgtaacg gcaaaccata acgtcgcaag ctctcttctt
     3481 ctagagaacg gaataattgt cctcttacac catccaatga aggacggaat tgatttaata
     3541 atttttgttt ctgttttttc ttcatatgtt ctctccttcg ctcataccct ttcgattata
     3601 agaaaaaaac agccaaatga aaaggagtaa tgcaaaaaaa cttgcgtata cgcaaattac
     3661 aacagaaaaa tttgcgtatt ttcccgtttt ttgaaagaaa aataaaaaat ggaacggatt
     3721 atgtatatat acataatccg ttccattctt tctatttttt cggtatcaag gcaaaaacag
     3781 gtagttcagg tgaataaatg acttcatgtt cgtgccaggg tttgaccatt agcgaatgat
     3841 cgaaccgttt tttcagttct tcgatgattt gatcatgtgt ccagtttctt gcaggaaaat
     3901 gttggatttt ctctgcatcg atcaaaaaga caaaatcgga atgaaaagtc tgggtaactt
     3961 cttccgagag agaattcaag cgttcgattg gatccataaa atcacctttc gacttaaaat
     4021 tgggtagtat ctgggtcttt tgctttgccg actacaccat ctaactgatc gatttgtttc
     4081 atttcttctt ctgttaattc aaaatcaaat aattcagtat tttctttgat tctgcttggt
     4141 gtcactgact taggaagagg aaggaattcg tgttgcagcg accaacgcaa agcaacttgt
     4201 gcaatcgtct tgtcatgtgc ttcagcgatt tgtttcattt caggcacatc gaagatttta
     4261 ccagtgccta atggactata tgcttcgagg atcatatcat gtttttttgc atactcaact
     4321 acagttgtct gtaattcgcc tggtgcaagg aaaacttgat tgaccatcgg cataattttt
     4381 gctgtttttg ctagtgtatc tagatggtgg ggtaagaaat tgctcacacc gattgctttg
     4441 attttacctg cttcatacag ttcttcaaat gctcgccatg tatctgcatt tgcttgttcc
     4501 caattatcac gaaaagcaac agggtttggc cagtggatca aaaacagatc taaataatct
     4561 gtttggagct tcttcaatga ttcttcaaaa gaagacatca caagttcata gctgtggttt
     4621 gcattccata attttgttgt caagaatatc tcttctctag gaattccact ttccttaatc
     4681 gcttggccga cgctttcttc attcttgtat ccttgggcag tatcgatatg gcgataacct
     4741 gcagccagcg cttctttaac agaggaaaca gcgacatccc catcaggtgt ttgccatgtg
     4801 ccgaatccta ctaccgggat ttcgtaacca tttgatagac gataagtgct tgttaatgaa
     4861 ttcatacttt cagctccttt tttattcgtt caactataag tgtagcgcat ttttcgaaaa
     4921 aaagaatcca ttttgtttct actcttgaaa tttttgttta aaagaaaatg attttgattc
     4981 tggtattttt gatttttttt ccgtaaacta agacagaagg gaatgagaaa atggatatct
     5041 atttaaaaaa agcaattctg catattattg acagagaaac tggttctcca gtcttttcac
     5101 aaaaagagct ggatctgaca aaagaatata ttcgtgattt tcttcaaaag aagatccaaa
     5161 aaatctcttc tgctcaaaca aaaactggac aactagcaga agaaagcatg atcgttgaat
     5221 cacttaaaat cgctcaagaa aattttgtgg aagcaagtga agcaatcgtt cagcggtggt
     5281 tcgatatcta ccaagaaagt gaagaagcac ctagtgcaga tgtatttgtt gtcttgtatg
     5341 aattagatac agtgatgtac ttatctttat tgaaagtcaa ttatcgtgag gcttacactc
     5401 actttgttga agcagacgaa gcagggatcg acaaccgctt gattttgaat cgagcaattc
     5461 taggggcgaa aacacaaaaa gcagatgaag cattcgctgt gaatttgact gattggacct
     5521 acgaattaat tgaaaaaaaa tatgagtttt ctggcaaaaa agagctttat ttttctggaa
     5581 gagtgatcga aagtgttcct gcaccttctt tagaagaaaa tgtaaaagtg atccaaaaag
     5641 tagccaagca tcttggaaaa aaatttgaga cagaagaatt tgatatcatg gctgatttga
     5701 aagaagctgt ctatgatacg atagaagaaa aaggtcggtt ggatcatgag atgattgcgg
     5761 aaaaagtctt caaggaaaac atcacggcaa aaatggcttt taaagaagaa gtacaagagc
     5821 aaggatttgt cccagaagca cctcctgtaa aagaagtaca agaaatttct gagaaaaaat
     5881 ttggaaaaca aaaattcaag ctatcaaacg ggatcgaact gatcgtacca gtagatgtat
     5941 atcgcaaccc agatttgatt gaatttgtga ataatccgga tggtacgata tccgtgatga
     6001 tcaaaaatgt ggatgaagta ttgaatcgtt tataaaaatg actagaatat taggtgttca
     6061 agtaccagtg atcagttaaa tgaattgtgt caagagaagg atcggaaaag acgaatataa
     6121 caaaaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
     6181 aaacggcgga agcagaagca actccttctt atttcttgga gttaaacgct tctgtcccag
     6241 cctcttttta tctaataaaa ttttaagacc atttactaat tatcgatttt ttctgccgca
     6301 tttttaggca cttcagaagc ctttatttct tcccagctta atacaccttt acgaggattg
     6361 actttgagtt taagatacgc attcatcttt aatggatgtt ctctttcttc gcgaagcgtc
     6421 acctgttttt tttcaccatc tttattataa gccgcttgct tgtaggtata agtcgtgaat
     6481 ttctctccgt tatctgcttg ggtagctgac atttcgccat tcgtcgtgat ttttgtataa
     6541 taagcttcgc cgccgtaaaa ataatgataa gctgcaaaac ttccgccgag aaatatgcct
     6601 aaaacgacaa gtacgccaat taattttttc atgtacttca cctctttcct tattattaac
     6661 gaaatagaag atttcgtcta ttgaaagttg ttggcaacag ctgaatttta aacttttcct
     6721 cattttgttc ttattgcttc aaaagaaagt taaggactat cttgaagttt ttctcaaaaa
     6781 acacattttt ttatttataa aaggtacttt tttcgcgtat aagacagacg agtgctctta
     6841 gttgactttg accaatattg acgtataata agcttagttc gaatcttaca aaaagtaagt
     6901 cggacgtatt ctttttgatt tagtcaaaaa aagactataa taaaaaggtg agagctgaaa
     6961 tttttcagtg atctatatta tttaggaggt ttgtaaatta tggcaaaaga acattatgac
     7021 agaagtaaac cacacgttaa cattggtacg attggacacg ttgaccatgg gaaaactact
     7081 ttaactgctg cgatcactac agtattaggc aaaaaaggcc tagcaaatcc tcaagactat
     7141 gctagtattg atgcagctcc agaagaacgt gaacgtggga ttacgatcaa taccgcacac
     7201 gtagaatatg aaacagaaaa acgccactac gcgcatattg atgcaccagg acacgcggac
     7261 tatgtgaaaa acatgattac aggggcagca caaatggatg gtgcaatctt agttgtttct
     7321 gcaactgacg gtccgatgcc tcaaacacgt gaacacattt tattgtcacg ccaagttggt
     7381 gtaaaatacc tgattgtttt cttgaacaaa gttgatttag tcgatgatga agaattgatc
     7441 gatttggtag aaatggaagt tcgcgagtta ttgagcgaat atggtttccc aggcgatgac
     7501 actcctgtga tcaaaggttc cgcattaaaa gcattgcaag gcgatccaga tgctgaagct
     7561 gctattatgg aattgatgga tacagtagat gaatatatcc caacaccaga acgtgataca
     7621 gataaaccat tactattgcc agtggaagac gtcttctcaa ttacaggtcg aggaactgtt
     7681 gcctcaggtc gtattgatcg tggtgctgtt cgtgtcggtg atgaggtaga gatcgtaggg
     7741 atcaaacctg aaacacaaaa agcagttgta acaggtgtag aaatgttccg taaaacgtta
     7801 gattacgggg aagctgggga taacgtaggc gtgttgttac gggggatcca acgtgacgat
     7861 atcgaacgtg gacaagtact tgctaaacca ggttccatta ctccacatac aaaattcaaa
     7921 gcagaagtgt acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcaacaac
     7981 tatcgtccac agttctactt ccgcacaact gatgttacag gaacaatcac attgccagaa
     8041 gatacagaaa tggtcatgcc tggtgacaac gtgacgattg atgttgactt gatccatcca
     8101 atcgctgttg aaaacggaac gacattctca attcgtgaag gcggacggac agtaggttca
     8161 ggtatcgtta cagagatcga agcataagaa acgcgactcg aatattttgg taatgaaaaa
     8221 tgtttaaaat aaaagagcgg tccaaacagg atttctgttt ggaccgctct ttgttcttca
     8281 attgtataag aagagattgt agaagcatga actttcgttt tacttcagtc tcttttattc
     8341 atcatgagag tgttcatcta tcggtatgca gtttttaata ctttcttcgt atccattttt
     8401 tcaaaccact tattctacct tgtgtctgct caactggcgc attatcaata atcagcaaag
     8461 caatcataac atatgcaatc caactatctc tagaatatag agtataacgt gagacccagt
     8521 ttgtagcgta cttgctttta tattctctta atccatggaa agaataaaaa tgagaaccaa
     8581 actcgtaaac caagtatgca atcctttcct gtaaaaaact ctttctggaa gttccgacat
     8641 ttgaaagagg ggccatccct aaattgaacc attggatatg ttcttctttc atataggcaa
     8701 acaaatgcaa gaacaagaaa tccatactgc cagaaggtgc ttttttagaa tcatatcgca
     8761 tcagatcaat cgttcctact ttattgttcg tgtaagtagg catgatcgtt gcaaaagcga
     8821 caatttcttc ttgggtattt ttgacaactg caataggcgc tctttgtaga taatcttcag
     8881 agaaaaagcc taaagaaaac cctttttcct ttcgtgaacc caaccaatta tcagagatat
     8941 ttttgaaagt actcatttgc tcagtagaaa atggtggttg taagacatca aatgtaaatc
     9001 cttctcgttc gatccgattc aagacagcac gagtgccctt catttttttc cctgaagttg
     9061 taaaactatt caggtctaca taagcttctt cccccatctt aatgaaatcg tagccgaatt
     9121 catgcaaaat cataacaatc tcttcgcttg tttcgtagaa aactggtaaa tagcatagtc
     9181 gatctgtttc ttcgataaaa gcttcgattg cttctggaaa atcttctttc ttaccagaag
     9241 gatcacccat tacaatacat ttattattga agcaagcgaa ttggagaaga acagttggtt
     9301 caccgtcttt ttcataggca aacatccgct tatcttttaa gaagatcaat tgactatcgg
     9361 agtttcctcc ataagttgta aggatcttca gggccttttc ttcattaaag gcttcgccaa
     9421 tctgtttctt ttctccttga aggaaatgaa caaatagaac gatcatgaaa ctaacagcga
     9481 tgatggccaa caatcccgaa aaccaaatct tttcagaagg aaataagaaa aaggaaataa
     9541 aatggtgacg ccgatgaggg aaatccggca gattgtatac cccaataacg atgtaaagca
     9601 aggacagtgc accaataatg attccatcga tcgtacgcca ctcccatgaa tagatcaatt
     9661 gttcccgaaa taattcgttt ttagaagcaa gaatgatcag aagcagaata gacaagaaaa
     9721 ccaccggtgt gaaactaaaa tcacttaata aaacataaag taaagctaga acaatcaaga
     9781 agattgtagg caaatatgct cgtttcacac gtgctgcgat ccctctgccc ataatgatta
     9841 aaagaaatcc caatagtata ctaggaaatt ggataataaa atgaaatttc aatgggttca
     9901 aactatgaag ccagcgaaac tctgtaaaag cttgcgggat agtggcagaa agtactagca
     9961 tgattccaga aaagtataat aagacaacca cgattttatg cgcaatttct gttgctaatt
    10021 gtttagggat accagaataa cgctgatcaa aagtggaacc gatatttttg aagaaaaaga
    10081 cgattccaat caaaaacgga atgatgtagt agaacaggcg atagagcaag atccacacaa
    10141 cgactatttc tcttggcaca ccaatagcgg acaacccgat aatcatcatt acatcgaaac
    10201 tcccaatttc tcctggaatc atagacacga ttcctatcac tgaagcagca atgaataatg
    10261 gaattgtttg ccacagatca atccggattt ccattaaata accaacagaa aggaaactaa
    10321 acaggacacc ggtccattcc aacaatgaaa ctagcaatag ctgtccttga gttttaagtg
    10381 aggtaccacc aaggaatcct ttctttttga aagtagtaaa taaaaagacg acaggaaaat
    10441 atagaccgcc gccgatcaac catatccaat attgcttcaa aaaaggagca actggcgtga
    10501 agactacaag taaaaaagaa agcagactgt aaatagaaag tccagccatc aaaaacaaga
    10561 aaactttcga taatgcttgg atcacgtttc tgctctcttt cttttgtcca tatagttcag
    10621 agcgaagccc cacactaaca aacccgccga aaccggcaat attgttgatc gtattgatca
    10681 gccagcttgt ctcaaacagg taacgaggat tctgcttttg ttcaagcatc cgattcaaaa
    10741 taatatcata accaaccata ggtaacacag agataagacc aatcaatagc attagtccgc
    10801 ttttccatac aggtatagtg gcaaacgttt ctcctaattg ctggatagat agagttttac
    10861 cgatggacat caattcaccg acaataatga caatgacaga aatcaaaaag attgttttga
    10921 acagtcctag gtggtttttc agccattgta tggtattttt taacaaataa atcaccttct
    10981 attcctaata gttatgtttc agaaagaaat tgaagaactt ctcgattgaa gcgttcagga
    11041 tcttttctag ctaattcatg tccttgttct ttcacaagca caaaggaagc ttttggtata
    11101 gttttagcta tatacaaaga atgttttagc ttgattacat ctttttttcc cacgatgatc
    11161 aaagtggggg agttgatttt ttttaaatca ttttctgtaa gcccgatgtc atgcagcaaa
    11221 agtttgataa ctaaaagatt tttgcgaaga cttgggcgaa ataaagagag taaccatacc
    11281 catgcataat gaatattact gatgactctg gcagaaaaac gaacgccttt aacaagcgtg
    11341 tttccagaat ttaaaatcaa tcgatgaact tttttaggga agctgctggc aaataccagt
    11401 gctagatttg ctccatcact aaatccaaga aaatctgcct gatcgatttt ttccagcaac
    11461 atgatcgtat tcagatcctc ggccattaac tgaaaattca acatagatgc ttcgtttgtc
    11521 gaacgaccat gtcctcggct atctactaga tacacggtat agtagcgttc gagtacaggg
    11581 acctgttctg aaaaaaagcg gccgctgccg tcattgccat gcagtaaaaa gagagggaag
    11641 ccttgaccgc tcttttcata gtaaatttcg ctcccatctg gcatttttgc atatttcttt
    11701 tcttttttca cataatcacc tactgtgtaa agtataacga ttatatcagt cattgggcaa
    11761 ttgtttttac tgaaaaacaa cgatcatttt cgtaagatgt caaaaaatac aggaaaattc
    11821 attttttctt aaggcgtaca cagaaatcaa ggaagaaata gccaagaaga taaagaaata
    11881 gtaagataaa aaaagatgtc aaaataacta ggtgatgaaa tgaagcgaaa acgattatct
    11941 attatccgta ttctttttta catgatcatt ctgacggtta ttggtgcggt gttgttttta
    12001 tgaggaagaa cttatcgttc ggtaaaacga gtggaaagct atcaaataga aataacaaca
    12061 gctgcaaaaa attatcatat ggaaaactac gagaatttga tcaaggcgat aatattgacc
    12121 gaatcaaaag gaaaagggac agatctcatg cagagctcgg aaagtctgca tggagaagct
    12181 gagatcattg ataatccagc tgacagtatt gatcaaggtg tcaaatattt ggcggaaatg
    12241 atagtagaag caaagaaaca aggatgcgat ttatggacag ctgtccaagc atataatttt
    12301 gggaaagatt atattgccta tgtaaaaaat catggcggag aaaacacagt agaattagca
    12361 gaagcgtatt ctaaagatgt gctgagccca cttttaggaa atgaggaagg gaaacaatac
    12421 cgatattggc ggatccagtc cttgctttac aatggcggct atctctatca taatggaggt
    12481 aacatgtttt atcctgatat tgtcaaaatg aatgaaaaaa taatagagac ctatgaaaaa
    12541 gtctttaagt aaaatagcag gaaatgttca atcatgaata aaattaaatg aaagaaggag
    12601 tgcgcaaata gtggaaaaaa ggcaaaaaat ggaaaatcaa gaagtggggt acacaaccaa
    12661 agactggatg ttacggtttg tgaagggaat gtttatcggt tcaggattta tcttgccagg
    12721 agtcagcggt ggtgcattag cagctgtctt tggcatgtat gagcggctga tttctttttt
    12781 agcccacctt acaaaaaatt tcaaagaaaa tgttttgttt tttctgcctg ttggtctagg
    12841 cggagtgaca ggcatctttc tactttcttt tttagtcagt tttttattag gtgcgtatga
    12901 aacaacgata ttatggtttt ttgtcggatg tatcgctgga actgttcctg cgttatggaa
    12961 agaagcaggg aaaaaaggaa gagatacttc tgatgtgatc atcatggttg ttacttttgt
    13021 tctggcttat ttgtttttat tatacggcgc tcaattattt gacgggcaag tcgatcagaa
    13081 ttttggaaca tggctgattg ccggcggatt gattggtttg ggtatgatcg tcccaggatt
    13141 gagcccttct aattttttgg tgtatatggg attgtataaa gcgatggctg acggatttaa
    13201 agaagtacga cttgaagtga ttgtgccgat cggaattggt gggattcttt gtgttcttgg
    13261 attgtcaaaa atcatggatt acattttctc aaaggcatat agcaagctat tccatttcat
    13321 tttaggtgta gtttttgctt caacgattat gatcattcct accgattacg cgggattggg
    13381 agttgctggg gtactagctt gtcctgttct ttttatagcc ggtacagcac taggttggtg
    13441 gatgagccgt ttggaggaac aatataagta aagatagatt gataataaaa acagcacacg
    13501 ggtgattcaa acaaaaaggt tggcatcttt tttgtaaaat cattcgtgtg tttttctata
    13561 aattttataa aaacaagttg tttttaatct tggaacacgc tagacttaaa cacgtgatta
    13621 tttttgaaag ttggcggaaa atggagaaaa agaaaatgaa tggacttaca cagcaagagg
    13681 tagttcagaa aatggcttct ggccagcaaa atgactacca ggaagatgca tcaaaaagta
    13741 caaaacaaat attcagcgat aatatattga cgttattcaa ttttctgaac tttggtattg
    13801 ggatttgtct gttatttgta ggtgcgtatt ccaacatggc gtatttagca attattgtcg
    13861 tcaatatcgt tattggaatt tatcaagaaa tacatgcaag aaatcttgta cggaaattat
    13921 cgattgtttc ccaatcagaa gtactagtga tccgggacgg taaagaacaa tcgatttcta
    13981 ctaaagagct ggtattagga gatttggtaa aaatcggagc aggagaacag ataccagccg
    14041 atttacaggt tatatctgga cgggcagaag caaatgaagc tcttttgaca ggcgaatctg
    14101 atttgattgt caaagaaact ggagctgaat tgctttctgg aagctttttg acaagcggtt
    14161 ctgtcatggc tgaagttatc cgagtaggtg cagataatta tgctgtccgc ttgactagcg
    14221 aggcaaagac tcacaaagct attcgttctg aacttgtaga ttcgattcgc aaagtatcca
    14281 aattcactag tttcgtgatt attcctttgg ggatcatact atttttggaa gcactgttca
    14341 tccgcgatgc ttctatcaaa atttctgtta ttgcatctgc agcagctttg ttaggcatgc
    14401 ttccaaaggg tttggtattg ctaataagta ttgcattaac gacaggggta accaaactgg
    14461 caaaaaaacg aatccttgtt caagacatgt attcaattga aacgctggcc catgtcgata
    14521 cattatgctt ggataaaaca gggaccatta ctgaagggaa aatgagtatc cagcgggtag
    14581 aagcactaca tccgctgtac gagaaagcca tcccagaaat tatggggacg tatctggcag
    14641 aaagcagtga taacaacatc actatgcggg ctatgagaga ttattttgag ctttctaatc
    14701 ggtatgatgt gacaaacaaa gtcgcatttt cttctgatcg caaatggggc gcgatggaat
    14761 ttccagaact gggagtcgtg tacgtaggag cgcctgaacg tttggtggaa gaagatcggc
    14821 ttccggacag tctaaaaaca gcccaagcga atggctatcg cgtacttatg ttggggatcg
    14881 ctccaaatca gcaattgaac gaagaaaaac cagcatttgt tgagccggtt gctctgtttg
    14941 aaatcgatga tccaatccga aaaaacgctc aagaaacact agcttatctt catgaggaag
    15001 gtgtggatct gaaagtgatt tctggagaca atccttttac tgtctccaat atcgctagac
    15061 gtgcaggatt accgaattat gatgcgtatg tcgatctttc tcaaattact gaagaaatgg
    15121 aagtgcgaga agctgctcat cgctatacgg tttttggacg agtgagtccg cagcaaaaga
    15181 aattgctggt aaatgaattg aaagaaagcg gcagaacggt tgcaatgaca ggcgacggtg
    15241 tgaatgatgt tttggcgtta agagaagcag attgcagcat tgctatggca gaaggtgatg
    15301 gagcaacacg tcagattgcg aatctggtat tgttggactc tgattttact acattgccag
    15361 aagtcctgtt tgaaggacgc cgagtggtca acaatgtgac gaaagtctca ggcatctttt
    15421 ttatcaaaac gatttattca tttattttat cgattatatg tgccgttaca gcaattggat
    15481 ttccgtttat ccctatccaa atcacgctga ttgatttagc gattgaaggg tatccttcct
    15541 tctttttatc ttttgaacaa gataaaagaa aaatcactta ccgttatttg cctacagcac
    15601 ttattaatgc tttaccaaat gccttgcttg tagtagtgaa tatcattgcg gtgtatctac
    15661 ttggaaaaac attcgcatgg ggaaatgctg aaactactac tttgatgtat tacttgctga
    15721 ttgggatcag ttgcatggca gttgtaaaag cttgttatcc cttcaaccca ttgcgcatct
    15781 tcctagcagt aacgacgatc gtagggatat atgtagctgc aatgctgttc catggaattt
    15841 tggaagtaaa cttagggttg ggagagagcg taggatactt ctttgtattc atgctcgtaa
    15901 atattgttct tcgagtattg tactggaagg ctgatattca aaatcagtta ctgaaaagag
    15961 tagcaaattg atgcttaatg agatagaaat gtgttatact tgaaaaagca atgaaatatg
    16021 atacaatcaa acatgtataa ttgtgtcctt taaaatcgca ctgatttcag ttagtgcgat
    16081 tttttaccgg ttattctcct gaaatatctt ctgttaaacc caaaagatat caatagagca
    16141 gggggtgaaa aaatggctca acggattcct caagaggtta tcgaaacagt acgcagtcaa
    16201 acgaatatcg ttgaggtgat tggacaatat gtccagttga aaaagtctgg taaaaattac
    16261 ttggggctgt gtccttttca tgaagaacgt acaccgtctt tttctgttgc agaagacaag
    16321 cagatttttc attgctttgg ttgtggtaaa ggcggaaacg tgtttacttt tcttcaagaa
    16381 ctagaaggtc taagttttcc agaagcagtg gtaaaagtgg cagacttcga acagataccg
    16441 ttagaagatc aatggcgtca gcgtactata gctgtccaaa atcctgaatc tgctacgggg
    16501 aaactgattg ctgctcatga aaaagcagcg gaaatctatc atcacatgct attgcataca
    16561 aaagctggac agccagcttt agaatatttg caaagtcgtg ggttgtcttt agaattaatt
    16621 gaagaattca acataggttt tgcacctaat gagcgttctt ttcttcagca agtattcaag
    16681 aatgaatcaa tttcaaatga attgttagaa cgctctggat tgtttgtcac gcatgatgat
    16741 ggccgtatgt ctgatcgttt ttatcaaaga gtgatgtttc cacttcgtaa ttctcaaggc
    16801 cagacgatcg gtttttcagg acgctggctt gcacctgaaa aagggcagga aaaagatcag
    16861 cccaaatact tgaatagtcc agaaacagaa ctgttcaata aaagacaagt tttatttaat
    16921 cttgataaag caagaagcga tatccggaaa aacggagaaa ttttgttgtt cgaagggttt
    16981 atggatgtga ttgctgcatg gcaggctggt atcaaaactg gattagcttc tatggggaca
    17041 agtctaacag ctcaacagat ccaacaaatg gaaaagctga cgaaagaatt ggtcttttgc
    17101 tatgacggag acaacgcagg ttttgaagca accaatcgag ggattgaatt gcttcggcaa
    17161 aacagtcgat tagcactttc tgtcgtagtc gttccagaaa agcttgatcc tgatgaatat
    17221 ctccgtaagt atggagaagc atctttccaa gaattagtgg ctcatgggcg cgaaacagtc
    17281 tttactttta aatcacgtta tttaaaacaa gggaaaaatt tagataatga aaaagacaag
    17341 atcgaatacc tcgaagaatt actagttgaa cttagccgag ttgtttcacc aatcgaacaa
    17401 gatatgtatc tatctcaact gtcaacagag tttcagatcg gcagagagac gatacaaaaa
    17461 caactgcgcg atcttcgaag gacaaatcag caaaatcgtc aagaacctca ggaaagtcaa
    17521 cacactcagg ttacgagaca agaaactgca cggaaaaaga gacctttgac gcaagtagaa
    17581 aaggcagagc agattctatt atatcgtgca tttaaagaag tttctgtccg taatatttta
    17641 aaagaacgag aatttcagtt tattcatgat gcatatgcag aagtttattt tttattcgat
    17701 gcatatgtct cacaacatta tgaattcaac cttgcagagt ttttagactt tttgcaagat
    17761 gaaaatttaa ggcggttggt ggtggaaatc gaatatttac cggtggcaga agaaagttcg
    17821 ccacgagaaa tccaagactt gctagatgtg atcagcaaat ctggcttagc tgatgaaata
    17881 acattaaaaa aacaaatgca gcaagaagcc agacgaactg gaaacaaaca gcttgagctt
    17941 gaattgacga ttgaaatcat caatttaacg aaacagttaa aacaagccaa atagctactc
    18001 aaggggggcc ttcttttatg gcaaatggaa cagataaaaa aacgtatgaa gcagcagtag
    18061 cagctttcat caaagaaaac aaacccaaac gacaagttgt atatgatgat ttatccaatc
    18121 agcttgctac accattcact ttgaatgcag atgaaatgga caaattgatc caaaagattg
    18181 aagacgcagg tatcagtgtg gtggatgaaa atggtgatcc atcaattcac agcttgaaga
    18241 gtgctgagaa aaaagctgaa caagcaaaaa cagaagatct ttctgcgcca accggtgtaa
    18301 aaatcaatga cccggttcgt atgtatctga aagaaattgg ccgtgtctcc cttttaactg
    18361 cggaagaaga agtagcgtta gcattgaaaa tcgaagaagg cgaccaagaa gcgaaacagc
    18421 gtctggcaga agcaaacctt cgtttagtag taagtatcgc taaacgttat gtaggacgag
    18481 gcatgcagtt ccttgacttg atccaagaag gaaacatggg actgatgaag gcagttgaaa
    18541 aatttgacta ccgcaaagga tttaagtttt caacctatgc tacatggtgg attcgtcaag
    18601 ccatcactcg agcaatcgct gaccaagcac gtacgatccg tattccagtt catatggttg
    18661 aaacaattaa taaattgata cgtatccaac gtcaattgct gcaagacctt ggaagagaac
    18721 ctacgccaga agaaatcggt gcggaaatgg atcttccaac agaaaaagtc cgagaaatct
    18781 tgaaaatcgc gcaagaacct gtttcccttg aaacgccaat cggtgaagaa gatgactctc
    18841 acttaggtga tttcatagaa gaccaggaag caacaagtcc agcagaacac gcagcttatg
    18901 agttactaaa agaacaattg gaagatgtac tagatacatt gactgatcgg gaagagaatg
    18961 tccttcgctt acgttttggt ttagatgatg gccggacgag aacattggaa gaagtaggga
    19021 aagtatttgg tgttactcgg gaacgtattc gtcagatcga ggctaaagca ttaagaaaat
    19081 tgcgccatcc ttctcgttca aaacaattaa aagatttctt agaataagcg tggactattg
    19141 accagaagag aaatcagtaa aactgtttgg tttattttaa aagaaaaaaa gcttgagact
    19201 acgcagtctc aagctttttt ttctatagga aggaaattca ttcattattt ttaagtaaat
    19261 cctaaaaata agctattggc gtgacgtata gaatatgata gattaaatgg tagcgtaaac
    19321 ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc aaattgcgga tcgaaaaaaa
    19381 tggagcagaa tactatctgt ccagactgcg gtcatcaaat aactgcagaa gatcagcaaa
    19441 taaaagaatc aattgaagaa acaaatacag atgcagctat gcaggataaa tcaactgaag
    19501 atgcagtctt tcctgataca gaattaaacg atccaatcga atggtcggaa ttgaaagatt
    19561 tacctctaga atctgtaatg gaattattcg aaaaagcaga accagaagac gaagaaccac
    19621 ctgctcacga tcaagtaact aatatagaga aaaatgaaca gaagaaagca actgttcatg
    19681 agacttcgga agaatcagga aagaatcaag aagaaacgct tgaatcaaaa caaaaaagaa
    19741 gagtagctga actgaaagaa atcgtcgata atgaggaaga aaattcgata ctatccgctt
    19801 atatcaaagc acatcgtgaa gatacaaaag aagagcacgc gaaagagctg ctcaaaatga
    19861 tcagtgaaaa aattgcacag gaaaacaaag tagagccaga aaaggaacaa gaaactgaaa
    19921 aagactctga tagagaaaac gccacaggta aagaaacagt aaaaataaaa gacgtaaaaa
    19981 cggatatcac agaaaaaact gaagtattta ctgaaaaaca attagaaaag gaaaaaacga
    20041 ataaactgcc ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa aagtcaacag
    20101 agcccgatga aataaatagt gaatccgatg ctgaaaatca atctgtaagc aaagagggta
    20161 ctgatcttga agcaaaaata gaaaaagaag agaaccagcc taagctgttt gaagcagaag
    20221 cgaaatcaat aacaatcgtg ccagatactg aaaaaacaca tcaagaatca aacttagaaa
    20281 aaacagatcc agataaagcg caaaagaaaa aatcaaaaaa agtaccttat ctcgttttag
    20341 cagcagtact tctactcggt ggaggaagct gggcttatta tgaccatcat caaaaagtag
    20401 aagccgaagt agcagcagaa gcaaaaagac agcaacaaat ggaagaactt catgagaaac
    20461 ttgctgcctt ttatacagat gatcaccatc aatttattcg tgcagatatg attcatcaag
    20521 atttatcaaa actaaaaaat gatttatcca gtcttaagaa tgaaaaagga tattcagagt
    20581 tagaaacaac ttaccaagat atccaatcta agatccgaga tatccaaaaa gtgaatgagt
    20641 ggttcatagc acctgccatc gtcgatgatc atctagcaga tgacatcaag ttgaaagcag
    20701 ataaagagat ccaagcaatc gaaacggata acacagattt tggaaaattg attcaacaag
    20761 cacaaacaga agctaaaaat caatatgctg aattgcaaac agctaaagaa aaaacagcta
    20821 tagtctttaa agacggaaaa gtagtggatt cagcaacacg cgaccaatac aagacggcaa
    20881 aagaagcagt agaaaaagtg aaaaatacag atctatctgc tcagttgaat gaacaattga
    20941 aacaagtaga taagatgctc actgataaag agaaaaaaga agaagaggaa aagaaaaaag
    21001 cagaagaagc gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa gcagcagctg
    21061 cagctcaagc cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa gcggcatcct
    21121 cgaccccttc ctcttcaaac tcagcgaatc agccaatcaa atctgccaga gcaagtgata
    21181 ttgccgatgc atctaatcca gcatggacgt gggcaccagg tgtccaaagc aaagtgattg
    21241 ctacatgtat ccaacgtgga tatatcgttg aaggaggtta cagattagaa aaagccagaa
    21301 tcgaaaatgg ggaaggctat tataatctat atgccacttc aacaaaatct gcattgatga
    21361 aaggcattgg agaaagtgct ttgccatttt acatcgtaac catcaactgt aaaactggat
    21421 ggttcggagg aaatgggagc aattaatttt aggcaaatga aagaggctgt gagcctgccg
    21481 tttaacaccg tttattcgaa aaaggggagt atgacacaac tgttgtcata ctcctttttt
    21541 cacctttcca aaccaaggct cagctattca agtagataac attatgttat gatagcaaag
    21601 aaatgtatta agaaaacctt tcactataga gggagaaaaa aatgaacgat ttattagcaa
    21661 ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc caaaaaaacg
    21721 gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa gcagttgaag
    21781 gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt cctaaaagcc
    21841 gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta ggtgcgtttg
    21901 tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg ccgacaatga
    21961 cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt gatgcaaaag
    22021 accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc aaatatggaa
    22081 cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa ttaacaggta
    22141 cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa cgatataatg
    22201 aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca gacggtgtac
    22261 tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca caaatgattt
    22321 taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa tcggatccag
    22381 aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca ttaggtaatc
    22441 ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc aaaaaatcta
    22501 attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat taacatagtg
    22561 ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa tggtcatgaa
    22621 tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt ttaagctgac
    22681 accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc atttatctgc
    22741 agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg ctactgttta
    22801 tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt ttaatgatgg
    22861 attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc atttactatg
    22921 cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag tggagcaagt
    22981 agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc atggaatctg
    23041 tcaggaatgc tatgaaaaag aaaaaa
//
LOCUS       DAXWLH010000032        22986 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXWLH010000032 DAXWLH010000000
VERSION     DAXWLH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.032"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(105..560)
                     /locus_tag="ACGE36_000909"
     CDS             complement(105..560)
                     /locus_tag="ACGE36_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383325.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="ACGE36_000910"
     CDS             complement(714..1922)
                     /locus_tag="ACGE36_000910"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFF8383326.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="ACGE36_000911"
     CDS             complement(2034..2366)
                     /locus_tag="ACGE36_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8383327.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="ACGE36_000912"
     CDS             2514..3389
                     /locus_tag="ACGE36_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8383328.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="ACGE36_000913"
     CDS             complement(3471..4052)
                     /locus_tag="ACGE36_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFF8383329.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="ACGE36_000914"
     CDS             complement(4058..5317)
                     /locus_tag="ACGE36_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8383330.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="ACGE36_000915"
     CDS             complement(5319..6365)
                     /locus_tag="ACGE36_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383331.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="ACGE36_000916"
     CDS             complement(6600..6875)
                     /locus_tag="ACGE36_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFF8383332.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /gene="der"
                     /locus_tag="ACGE36_000917"
     CDS             complement(7118..8428)
                     /gene="der"
                     /locus_tag="ACGE36_000917"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFF8383333.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="ACGE36_000918"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="ACGE36_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFF8383334.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /gene="cmk"
                     /locus_tag="ACGE36_000919"
     CDS             complement(9974..10654)
                     /gene="cmk"
                     /locus_tag="ACGE36_000919"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFF8383335.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="ACGE36_000920"
     CDS             complement(10703..11338)
                     /locus_tag="ACGE36_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFF8383336.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="ACGE36_000921"
     CDS             complement(11404..12807)
                     /locus_tag="ACGE36_000921"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFF8383337.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="ACGE36_000922"
     CDS             complement(12794..13825)
                     /locus_tag="ACGE36_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383338.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="ACGE36_000923"
     CDS             13856..14077
                     /locus_tag="ACGE36_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFF8383339.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="ACGE36_000924"
     CDS             complement(14242..14979)
                     /locus_tag="ACGE36_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFF8383340.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="ACGE36_000925"
     CDS             complement(15132..16472)
                     /locus_tag="ACGE36_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8383341.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..18232)
                     /locus_tag="ACGE36_000926"
     CDS             complement(16709..18232)
                     /locus_tag="ACGE36_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8383342.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18610..19221)
                     /locus_tag="ACGE36_000927"
     CDS             complement(18610..19221)
                     /locus_tag="ACGE36_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8383343.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19344..19469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19551..20267)
                     /locus_tag="ACGE36_000928"
     CDS             complement(19551..20267)
                     /locus_tag="ACGE36_000928"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8383344.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="ACGE36_000929"
     CDS             complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="ACGE36_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFF8383345.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20826..21596)
                     /locus_tag="ACGE36_000930"
     CDS             complement(20826..21596)
                     /locus_tag="ACGE36_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFF8383346.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21593..21964)
                     /locus_tag="ACGE36_000931"
     CDS             complement(21593..21964)
                     /locus_tag="ACGE36_000931"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383347.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="ACGE36_000932"
     CDS             complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="ACGE36_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFF8383348.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6443 a         4913 c         3899 g         7731 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    18241 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    18301 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    18361 cctattccct cctctttttt cagtttttta aagctttctt ctttttcatt gtagcgtttc
    18421 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    18481 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    18541 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    18601 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    18661 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    18721 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    18781 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    18841 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    18901 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    18961 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    19021 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    19081 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    19141 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    19201 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    19261 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    19321 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    19381 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    19441 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    19501 acatatctat tcaaaaaacg caacatctaa caattataaa aagtaagtgt tcattttgag
    19561 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    19621 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    19681 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    19741 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    19801 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    19861 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    19921 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    19981 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    20041 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    20101 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    20161 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    20221 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    20281 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    20341 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    20401 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    20461 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    20521 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    20581 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    20641 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    20701 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    20761 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    20821 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    20881 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    20941 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    21001 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    21061 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    21121 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    21181 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    21241 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    21301 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgccgaa ttcttggaca
    21361 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    21421 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    21481 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    21541 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    21601 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    21661 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    21721 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    21781 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    21841 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    21901 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    21961 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    22021 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    22081 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    22141 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    22201 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    22261 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    22321 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    22381 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    22441 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    22501 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    22561 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    22621 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    22681 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    22741 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    22801 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    22861 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    22921 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    22981 tctttt
//
LOCUS       DAXWLH010000033        22660 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXWLH010000033 DAXWLH010000000
VERSION     DAXWLH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.033"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            47..1336
                     /locus_tag="ACGE36_000933"
     CDS             47..1336
                     /locus_tag="ACGE36_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8383349.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1462..2541)
                     /locus_tag="ACGE36_000934"
     CDS             complement(1462..2541)
                     /locus_tag="ACGE36_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8383350.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2650..3090)
                     /locus_tag="ACGE36_000935"
     CDS             complement(2650..3090)
                     /locus_tag="ACGE36_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8383351.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3147..3557)
                     /locus_tag="ACGE36_000936"
     CDS             complement(3147..3557)
                     /locus_tag="ACGE36_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8383352.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3738..4172)
                     /locus_tag="ACGE36_000937"
     CDS             complement(3738..4172)
                     /locus_tag="ACGE36_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8383353.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4272..5120)
                     /locus_tag="ACGE36_000938"
     CDS             complement(4272..5120)
                     /locus_tag="ACGE36_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8383354.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5509..7275
                     /locus_tag="ACGE36_000939"
     CDS             5509..7275
                     /locus_tag="ACGE36_000939"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFF8383355.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7377..8636
                     /locus_tag="ACGE36_000940"
     CDS             7377..8636
                     /locus_tag="ACGE36_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8383356.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8783..10084
                     /locus_tag="ACGE36_000941"
     CDS             8783..10084
                     /locus_tag="ACGE36_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383357.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10087..10938
                     /locus_tag="ACGE36_000942"
     CDS             10087..10938
                     /locus_tag="ACGE36_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8383358.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10951..11766
                     /locus_tag="ACGE36_000943"
     CDS             10951..11766
                     /locus_tag="ACGE36_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFF8383359.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11917..13083
                     /locus_tag="ACGE36_000944"
     CDS             11917..13083
                     /locus_tag="ACGE36_000944"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8383360.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13313..13960
                     /locus_tag="ACGE36_000945"
     CDS             13313..13960
                     /locus_tag="ACGE36_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8383361.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14044..14988
                     /locus_tag="ACGE36_000946"
     CDS             14044..14988
                     /locus_tag="ACGE36_000946"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFF8383362.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15123..16574
                     /locus_tag="ACGE36_000947"
     CDS             15123..16574
                     /locus_tag="ACGE36_000947"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFF8383363.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16595..17431
                     /locus_tag="ACGE36_000948"
     CDS             16595..17431
                     /locus_tag="ACGE36_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8383364.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17431..18090
                     /locus_tag="ACGE36_000949"
     CDS             17431..18090
                     /locus_tag="ACGE36_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8383365.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18216..18905
                     /locus_tag="ACGE36_000950"
     CDS             18216..18905
                     /locus_tag="ACGE36_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383366.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18911..19867
                     /locus_tag="ACGE36_000951"
     CDS             18911..19867
                     /locus_tag="ACGE36_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFF8383367.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19911..20423
                     /locus_tag="ACGE36_000952"
     CDS             19911..20423
                     /locus_tag="ACGE36_000952"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383368.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20679..21227)
                     /locus_tag="ACGE36_000953"
     CDS             complement(20679..21227)
                     /locus_tag="ACGE36_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8383369.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21316..22497)
                     /locus_tag="ACGE36_000954"
     CDS             complement(21316..22497)
                     /locus_tag="ACGE36_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFF8383370.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         7658 a         4102 c         4360 g         6540 t
ORIGIN      
        1 actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac
       61 catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca
      121 cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat
      181 tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca
      241 aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc
      301 agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt
      361 gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt
      421 aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt
      481 aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga
      541 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
      601 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
      661 aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga
      721 tgtagaccca gaagaaaaac cggatgtgac tccagaacca gatacggatt ctggaaacca
      781 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
      841 ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
      901 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaactaga
      961 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
     1021 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
     1081 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
     1141 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctag
     1201 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
     1261 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
     1321 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
     1381 tatccagaaa acgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
     1441 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
     1501 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
     1561 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
     1621 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
     1681 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
     1741 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
     1801 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
     1861 caacgtgttt aaaagagcga taacaaattg agcttctaat accaaaccaa atgtattgac
     1921 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
     1981 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
     2041 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
     2101 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
     2161 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat
     2221 atcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
     2281 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
     2341 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
     2401 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
     2461 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
     2521 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa
     2581 aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat
     2641 ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag
     2701 atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg
     2761 aataccttct gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat
     2821 tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag
     2881 taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt
     2941 ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg
     3001 cttgaggagg gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca
     3061 tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt
     3121 atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata
     3181 tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc
     3241 gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc
     3301 gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt
     3361 ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac
     3421 aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg
     3481 aatgtacgat cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta
     3541 gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac
     3601 acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca
     3661 tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac
     3721 taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt
     3781 acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag
     3841 ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt
     3901 aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt
     3961 ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat
     4021 ctcgtgccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca
     4081 atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt
     4141 aatacaaaat ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac
     4201 agttactaga ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt
     4261 tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg
     4321 ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa
     4381 aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct
     4441 tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct
     4501 tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata
     4561 taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt
     4621 ccaaaaacaa acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa
     4681 gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa
     4741 gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca
     4801 gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa
     4861 aacaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt
     4921 accaacataa cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct
     4981 tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac
     5041 atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata
     5101 aattgtctcg tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta
     5161 ttataactta cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa
     5221 attattatgt ttcattcttc tagaatatac ttctacgtta aaaactgtta aaaaactttt
     5281 actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag
     5341 gaagtagatt aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa
     5401 aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgtttct
     5461 ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct
     5521 gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt
     5581 attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctttg tggagatcca
     5641 tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt
     5701 accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaaggcg cttacagtat
     5761 gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt
     5821 ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat
     5881 caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt
     5941 cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga
     6001 agtaaagatc cagaccgaga agacttcttc gggggagatt tgcaaggtgt gagggatcat
     6061 ttggattact tgacggacct tggcgtcaat ggcatctatt tttgtcccat atttaaagcc
     6121 acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat
     6181 aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc
     6241 gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac
     6301 ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat
     6361 cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact
     6421 ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc
     6481 gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa
     6541 gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc
     6601 tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat
     6661 gcagtgatga attatgcatt cacagattcg attaaagatt attttgcaaa aaagaaaatc
     6721 acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg
     6781 aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt gaccctttgc
     6841 caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct
     6901 ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc
     6961 aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa
     7021 cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta
     7081 atagtatatg atcgtgaaga taagctctat ttcacacggg aactagaggg acagatcatc
     7141 tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt
     7201 cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt
     7261 ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt
     7321 tgtgcccatt ataataaggg tgtaacagca cgttgaatac acaggaggaa atgaagatga
     7381 aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat
     7441 tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt
     7501 taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg
     7561 aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat
     7621 cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc
     7681 ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag
     7741 cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg
     7801 cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa
     7861 cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag
     7921 gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg
     7981 caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga
     8041 ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc
     8101 caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca
     8161 ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata
     8221 tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg
     8281 gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat
     8341 ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa
     8401 tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag
     8461 ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag
     8521 tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag
     8581 aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa
     8641 aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga
     8701 gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag
     8761 caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa
     8821 aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa
     8881 aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt
     8941 tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt
     9001 tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg
     9061 tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa
     9121 tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac
     9181 acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt
     9241 cactgcattt actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt
     9301 aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc
     9361 agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg
     9421 tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat
     9481 cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat
     9541 attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc
     9601 ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat
     9661 tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt
     9721 acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca
     9781 gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac
     9841 acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca atggtggtcc
     9901 cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact
     9961 tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt
    10021 catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga
    10081 ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt
    10141 acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca
    10201 agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg
    10261 actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat
    10321 acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc
    10381 tatacttata gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg
    10441 attcagatgg taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta
    10501 gaagcgctcg ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg
    10561 aatacttggc tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct
    10621 aaacttgatg gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa
    10681 ccgatgctcg ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta
    10741 gctaagttcc tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt
    10801 atttctgatg tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca
    10861 ctaccgattt gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct
    10921 ggcggaacaa aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc
    10981 gcattacgta cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt
    11041 ttcttgatat ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc
    11101 aatcagatcc aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa
    11161 caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa
    11221 caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa
    11281 tccgatcatg gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat
    11341 aaatctggta tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta
    11401 cgtacgcccg aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca
    11461 acaatcattt tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc
    11521 attttattcg gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt
    11581 catacattca aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca
    11641 gcagcagttg tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg
    11701 ataactgtct tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc
    11761 gaatgacaag tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta
    11821 gtttttgaaa aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat
    11881 taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat
    11941 caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg
    12001 aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc
    12061 ccaatgaaag agattacaag taacgcttct ttggcactag gagaagccta tatggatcat
    12121 cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac
    12181 agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa
    12241 caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt
    12301 tggctagatc caactttgac ttattcttgt gcttatttca atacacctga agatacgttg
    12361 gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc
    12421 gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag
    12481 tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa
    12541 agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa
    12601 ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct
    12661 gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct
    12721 ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat
    12781 attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac
    12841 gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat
    12901 tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc
    12961 tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc
    13021 gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca
    13081 taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct
    13141 ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc
    13201 tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt
    13261 cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa
    13321 aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac
    13381 caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc
    13441 ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc
    13501 aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa
    13561 atacccagat actgcaatta ctagtttgga attagatagt gattggggat cttacttcta
    13621 caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga
    13681 aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa
    13741 aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc
    13801 cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac
    13861 tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag
    13921 ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag
    13981 agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt
    14041 tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca
    14101 tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga
    14161 gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat
    14221 tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat
    14281 tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt
    14341 ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa
    14401 agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca
    14461 caactagctt acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt
    14521 tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg
    14581 gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat
    14641 ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc
    14701 ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca
    14761 cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca
    14821 tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa
    14881 gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga
    14941 tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa
    15001 ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa
    15061 gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata
    15121 atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag
    15181 aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg
    15241 tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag
    15301 ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc
    15361 tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg
    15421 tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg
    15481 gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg
    15541 aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc
    15601 ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat
    15661 tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat
    15721 ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat
    15781 tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc
    15841 aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact
    15901 cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag
    15961 aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt
    16021 attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc
    16081 agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc
    16141 agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg
    16201 tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg
    16261 cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca
    16321 gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga
    16381 agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta
    16441 ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag
    16501 aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa
    16561 atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct
    16621 aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga
    16681 taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact
    16741 gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc
    16801 aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg
    16861 acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac
    16921 aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat
    16981 gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa
    17041 agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt
    17101 agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac
    17161 agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat
    17221 ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa
    17281 agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga
    17341 tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa
    17401 agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ctctaattga tttttccctc
    17461 atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc
    17521 gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat
    17581 tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca
    17641 ctagtcctgc tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct
    17701 tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca
    17761 ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt
    17821 ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt
    17881 aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa
    17941 atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt
    18001 ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta
    18061 gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca
    18121 ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa
    18181 aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa
    18241 tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca
    18301 cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa
    18361 gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca
    18421 aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag
    18481 taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag
    18541 aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg
    18601 ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa
    18661 aagcaaagca aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat
    18721 tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt
    18781 tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc
    18841 atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg
    18901 agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa
    18961 agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg
    19021 tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc
    19081 tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg
    19141 gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag
    19201 tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga
    19261 tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag
    19321 tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag
    19381 aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt
    19441 tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt
    19501 cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg
    19561 caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt
    19621 gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact
    19681 cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg
    19741 actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct
    19801 agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat
    19861 ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc
    19921 ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt
    19981 tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga
    20041 ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg
    20101 gaacagctgc attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga
    20161 aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag
    20221 gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata
    20281 tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt
    20341 ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag
    20401 catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca
    20461 ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa
    20521 aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt
    20581 acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac
    20641 gatctctcgg cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt
    20701 attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata
    20761 gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc
    20821 tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta
    20881 ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct
    20941 gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa
    21001 ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga
    21061 ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt
    21121 gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa
    21181 gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt catttctcct
    21241 ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact
    21301 ttgctttttt gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa
    21361 agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt
    21421 taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag
    21481 gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc
    21541 tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac
    21601 tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat
    21661 ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc
    21721 aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta
    21781 ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc
    21841 tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt
    21901 caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat
    21961 cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag
    22021 gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc
    22081 tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac
    22141 agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat
    22201 aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc
    22261 ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc
    22321 caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag
    22381 ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat
    22441 caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat aactcaaata
    22501 atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt
    22561 tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa
    22621 gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
//
LOCUS       DAXWLH010000034        22525 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXWLH010000034 DAXWLH010000000
VERSION     DAXWLH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.034"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            177..2390
                     /locus_tag="ACGE36_000955"
     CDS             177..2390
                     /locus_tag="ACGE36_000955"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFF8383371.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSNLKSIVDKIKAVPDVYSVRRTNG"
     gene            2401..2847
                     /gene="dtd"
                     /locus_tag="ACGE36_000956"
     CDS             2401..2847
                     /gene="dtd"
                     /locus_tag="ACGE36_000956"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFF8383372.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            3018..4277
                     /locus_tag="ACGE36_000957"
     CDS             3018..4277
                     /locus_tag="ACGE36_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFF8383373.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(4366..5313)
                     /locus_tag="ACGE36_000958"
     CDS             complement(4366..5313)
                     /locus_tag="ACGE36_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8383374.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            5707..6999
                     /gene="hisS"
                     /locus_tag="ACGE36_000959"
     CDS             5707..6999
                     /gene="hisS"
                     /locus_tag="ACGE36_000959"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFF8383375.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            7014..8783
                     /gene="aspS"
                     /locus_tag="ACGE36_000960"
     CDS             7014..8783
                     /gene="aspS"
                     /locus_tag="ACGE36_000960"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFF8383376.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            8892..9767
                     /locus_tag="ACGE36_000961"
     CDS             8892..9767
                     /locus_tag="ACGE36_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8383377.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            10033..11916
                     /locus_tag="ACGE36_000962"
     CDS             10033..11916
                     /locus_tag="ACGE36_000962"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8383378.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(11991..12275)
                     /locus_tag="ACGE36_000963"
     CDS             complement(11991..12275)
                     /locus_tag="ACGE36_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8383379.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEDAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(12265..12999)
                     /locus_tag="ACGE36_000964"
     CDS             complement(12265..12999)
                     /locus_tag="ACGE36_000964"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFF8383380.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            13128..13751
                     /locus_tag="ACGE36_000965"
     CDS             13128..13751
                     /locus_tag="ACGE36_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFF8383381.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            13943..15064
                     /locus_tag="ACGE36_000966"
     CDS             13943..15064
                     /locus_tag="ACGE36_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8383382.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            15071..18199
                     /locus_tag="ACGE36_000967"
     CDS             15071..18199
                     /locus_tag="ACGE36_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8383383.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            18218..21412
                     /locus_tag="ACGE36_000968"
     CDS             18218..21412
                     /locus_tag="ACGE36_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFF8383384.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(21558..22265)
                     /locus_tag="ACGE36_000969"
     CDS             complement(21558..22265)
                     /locus_tag="ACGE36_000969"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8383385.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         7588 a         3984 c         4787 g         6166 t
ORIGIN      
        1 ttgtataata gaaacaatta tttatacaat ggatacgaaa aatcgtttga aaaagctttt
       61 aaaaagcgaa catttccgta aaaaaacgta taactgcacg gattttggga caattatcaa
      121 acagatattt gtcccgattc ccgttttacc gaaaaaagaa agggggatcg attcctgtga
      181 caaaagaaga aattttaacg ggacccggtg tcataaagct cgttagtcaa tacatgggtc
      241 ccgaacatgt agcatttgtt gagaaagcat gcgaatacgc gactgctgca catgacggac
      301 agtttagaaa atcaggcgaa ccctacatta ttcatcctat ccaagtcgca ggtatattag
      361 cagatttaaa aatggatccc catacagtgg ctacaggctt cttacatgat gttgttgaag
      421 atacagaaat cactttagaa gatctgagag aagaatttgg cgatgacgtt gctatgttag
      481 tggacggcgt aaccaaatta gggaaaataa aatataaatc ccacgaagag cagctagcag
      541 aaaaccaccg aaagatgctg ctagcaatgg ctcaagattt acgagttatc atggtcaaat
      601 tagctgatag actacataac atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa
      661 ggatcgctca ggaaacttta gaaatctatg cgcctcttgc acatcgtcta gggatcagcc
      721 ggatcaaatg ggaattagaa gatacagcac ttcgttatct aaatccaaaa cagtattacc
      781 gtatcgtcca cttgatgcag acgaagagag aagaacgtga aaaatacgta agtggtactg
      841 ttgaagatat tcgaatagcg acagaagagc tggggatttt tgcagaaatc tatggacggc
      901 caaaacacat ttattcaatc tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa
      961 tttatgactt gctagctatt cgagtgatcg tagattcaat caaggattgt tatgctgtat
     1021 taggagcaat ccacacaaaa tggaaaccaa tgcccggcag atttaaggat tatattgcca
     1081 tgccaaaagc taatatgtac caatctttgc atactaccgt gatcggtccg gcaggaaatc
     1141 cggtagaaat tcaaatccga acacaggaaa tgcatgaaat cgctgaattc ggggttgctg
     1201 cacactgggc ctataaagaa ggaaaaaatg aaaaagtaga accagatggt atgacgaaac
     1261 aattaagctg gttccatgag atactcgaac ttcaagacga aagctatgat gcttctgaat
     1321 ttatggaagg cgtaaaagga gatatcttta gtgataaagt ctacgtcttc acaccaaaag
     1381 gagacgttac tgagttacca aaaggatccg gaccattgga ctttgcatac agtatccata
     1441 cagatatcgg taacaaaacc actggtgcaa aagtaaatgg caaaatggtg cagcttgatt
     1501 acaaattgaa aaacggagat atcattgaga tcatgacttc tccaaattca tttggcccaa
     1561 gtcgcgactg gttgaaatta gttgctacta gcaaagcaag aaataagatc aaacgtttct
     1621 tcaaagccca agatcgagaa gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta
     1681 ttacagatct aggatttacg cctaaagata ttttgacgaa gaacaaactg caagaagcac
     1741 tcgatcgttt taattatcaa acagaagatg atctctatgc agctgtaggg tatggagaag
     1801 ttagcccctt gacgatggcc aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg
     1861 agcagcaaaa gcaagaagca gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa
     1921 tgaaagtacg tcatgaaggt ggcgttgtca ttcaaggggt agaaaactta ctaattcgta
     1981 tcagtcgctg ctgtaatccg attcctggtg atgatatcgt tggttatatc actaaaggca
     2041 gagggatatc cattcatcgt cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag
     2101 cagaacgttt gattgaagtc gaatgggaag atacatcgaa tacacgaaaa gagtatgatg
     2161 cagatttgga aatttacggc tataatcgtt caggcttatt gaatgatgta cttcaaacag
     2221 tcaatgcgct aacgaaaaat ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg
     2281 cgacgatcca tttgacggtt ggtatccaga atttatccaa cctaaagagt atcgtggata
     2341 aaatcaaagc agtacctgat gtctacagtg tacgccggac gaatggatag gaggacagat
     2401 atgagagcag ttgttcaacg ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt
     2461 ggaaaaattg gctacggcct acttgtttta ttaggcattc atgaaaccga tacgcaaaaa
     2521 gatgtggact atctggtaaa aaaaattgca caaatgcgta tttttgaaga tgaacaagga
     2581 aaaatgaata ttagtgtaga agatgtggga ggacaaattt taagtatctc tcaattcaca
     2641 ctgtttgcgg atacgaaaaa aggaaatcga ccaagctttg tggaagccgc acgaccagaa
     2701 acagctattc ctttatacga atcattcaat gaaggaatca gaagtcgcgg catcacagtc
     2761 gctaccggac aattcggagc agatatgtcg gtttctctcg tcaatgatgg acctgtaacg
     2821 atcattattg attctaaaaa caaataaagt aatctggttt tcactagtaa aaactatgaa
     2881 acacgtcgct taagttagac tgataaactc taacttaagt ggcttttttg tctgcaaaaa
     2941 gcgcttcttt tgtcagcagt cggaaaaaaa gttaagctta aattatacaa gttatttttt
     3001 ataggaggtt gctgaagatg aaagaagtac cagtagtaga tatcaaaatc acagatttaa
     3061 aaaaattgta tcaaaaagga tacaacatgt tggaggagtt acgccatgaa gcggacgctc
     3121 ccgtcgtcaa agcaaagatt ttcaataaag aagctatcac gatctacgga tcatcagcag
     3181 caaaagtatt ttatgatcca aggaatttca aacgaaaagg tgctatgcca aaattagtat
     3241 tgaagacact ttttggtcaa ggtggagtac agactttgga tggagctgct catcaccatc
     3301 ggaaaaatat ttttatggat ctaatgacac cggaaagaat ggaagattat catcgtatct
     3361 tagataagaa tctgacacaa gcacttgaag cacagcatgg tcaatttgaa ctatttgatt
     3421 taagtaagat ggttttcttt acatctattt gcgagtgggc aggtatcaat ttatcagcga
     3481 tttctaaaga tgaggtcgaa aaattagcag aatatcaaat ctctatgatc agcggaacgt
     3541 ttacttctcc gattgatcac ataaaaggtg tcgaaaatcg caaaaaatca gaaaaatggg
     3601 cgcaaggctt gattgaagaa gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat
     3661 tatatgcttt tgccaatgcc acagatttag atggtcagct tttacctctg gaagtggcag
     3721 ctgttgaatt attgaacatc atccgtccaa cggttgctct aactgtgtgg gcggcattga
     3781 tgggacatgc acttttcagt agaccagatt tgtatcagca attaaaaaat gacttttcga
     3841 cgttacaaga tccgtttatt caagaaatga gacgctatta tcctttcttc ccaatgctac
     3901 ctgcgatttc gctgaaagaa gtagaagtag atggttatcg tataccagaa ggcagttggg
     3961 ttattttaga tttatatgga acagaccatg atgaacgaac agttgaagcg ccagattcct
     4021 ttatgatcaa acggtatgta gggaaagcaa aagatatttc ctataaagaa gaatatgaga
     4081 tgattgcaca aggcggggga aattttcgac aaatgcatcg ctgtgcagga gaatggatca
     4141 ctcttcatag tctacgtgtg ttttccgatc agctagtaaa taagtttgag ttctctgtac
     4201 ctgaacaaga ttggacgatt ccattcaatc agtttccgac ttatccgaat agccgagcgc
     4261 tactttacaa gaattagatt agcataaact tatcgaaaaa aaggaagatc aaacagtgct
     4321 gatcttcctt ttttcatatt actataaatt gataaggtca attgatcaaa atccacgaaa
     4381 tgcttcatca atggtttgat aatcttcatt ggataaatgc aaatctaaag cttggacatt
     4441 gtcagatact tgctctggac gcttagctcc aggtataact acagacactc gtggattctt
     4501 catgtaccaa gctaaaacaa gttgcgcagg agtggcttgg tatcttttag caatggggcg
     4561 aagtacatcg acagcggtta aaatttctct gaaacgttct ccttggaaat ctggatttct
     4621 ttttcgagga tctccttcac caaattgttt ttcctcacca agttcatatt ttcctgtcag
     4681 caaaccggaa gcaagaggaa aatagggaac aaaactgatt ttgttttttt caagataagg
     4741 gaataattct ttttcagcct gccgatggat caaactgtat ttatcttcta ctacatcgac
     4801 atatccatct gcattcgctt ctttcaattg ttctaacgtg aagtttgaca caccgactgc
     4861 tcggattttt cctgcttctt ttaattcgtg caatgtagca actgattcat tttttggtgt
     4921 gctttcatcc ggaaagtgga tataaaaaat gtcgatgtaa tcagtttgca gacgttttag
     4981 tgcatcttcc accgattgtt tgagaaactc aggagaatta tcaaatgttc gccctttatt
     5041 tggtacatga gcagcttttg tagcgatgac gacacgtgag cgatcatatt ctttttcctt
     5101 cagtacctcg ccaatcagtt cttcggaacg gccgtttccg taagcataag cggtatcgat
     5161 caaattgatc ccgctattca aagctgtacg aactacttgt tttcctgtct catcttccaa
     5221 accgtcaaaa agattatgtc ctccaacggc atttgctcct aatccaagct gttctgcgta
     5281 gacttgtgaa tgtccgattc ttatttctgc catagctgat tcctcctttt tgtctagtct
     5341 aaaacaaaat accaaaaagc tgaactaaaa cgtttttgtg aggggaaata gtcagatatc
     5401 tcttgacttt gagactggtt tttagtaggt tataagtaat atcgaatgtc aatgaaagag
     5461 cgagtaagtt tttgaaactt tacaagagag gatcatcgag gctgaaaatg atctaaagtt
     5521 tcattagcct aaagacactc tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt
     5581 tattcgtaag aggaaagaga aatctttcaa aattaggtgg taccgtgtat gaatttcatt
     5641 cacccttgtt catgatgaac aagggtgatt tttttattta catagctaag aaagggagag
     5701 aaaactatga gtttccaaag accaaaagga acaaatgata ttttgccagg cgaatcagaa
     5761 aaatggcagt ttgtcgaaga aacagcacgt ttattattca gagattatca gtataatgaa
     5821 atgcgtacac cgatttttga acactatgaa gtgatatccc gaagtgtagg ggatacaacg
     5881 gatatcgttt caaaagagat gtatgatttt tatgacaaag gggaacgcca tgtgacgctt
     5941 cgaccagaag gtacagcacc aatcgttcgt tcttttgttg agcataaatt gttcggccca
     6001 gagtatgcga aaccgttcaa aactttttat atgggaccaa tgtttcgcta cgaacgccct
     6061 caaaaaggac ggttacgtca attccatcaa atcggagtag aagcatttgg atcagaaaac
     6121 cctgcaactg atgtggagag catggtgatg gcacttgatt tcttcaaaca gttggggatc
     6181 acgcaaatcc ggctagtcat caattcatta ggtgataaag aaacgcgtca agcctatcgt
     6241 caagcattga tcgactatct tttacctttt gaatcagagc tgagtgaaga ctctaaacgc
     6301 cgccttcatg aaaatccatt acgcgtattg gacagtaaag acaaacgaga tcaaaaattt
     6361 gtggcagaag ctccatctat cttagattat ctaagcgagc ctgctcaaaa acattttgat
     6421 accgttgttt ccatgctgga tgcattggct gttccttatg aaatcgacag taatatggtt
     6481 cgcggactag attattacac acatacgatt tttgaaatca tgagtgacgc accaaaaatg
     6541 ggggcacaag caacgatttg cgcaggcgga cgttatgaca atctagtgga agaactaggt
     6601 ggtccttcaa cacctggctt cggatttgca atgggtatcg agcgattact cttgactatg
     6661 gaggcagagg aagtagttat ccctgcattt aatgagttgg atgcttacgt ggtcgcactc
     6721 ggagacgaaa caaatatcga agcattgaaa gtggtccaag cgattcgaaa ctttggtttt
     6781 tcagctgacc gcgacttcat gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa
     6841 gcgaatgcca aattagtatt ggtgatcggt gaagatgagc ttgctagtca aacgattaat
     6901 gtcaaagcaa tggcaagccg aaaagaaaaa agttttccat tagaagagat atatgaacat
     6961 tttgatgaag tctatgatga aatgacttta ttcgagtagg aggaaaaaaa taaatggcac
     7021 aaagaacgat ctattgcgga ttagtttccg aacaacaatt agaacaaaca gttacattaa
     7081 aaggatgggt acaaaaaaga cgagaccttg ggggagtaat ctttatcgac ctacgtgatc
     7141 gtgaaggaat cgttcaaatc gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg
     7201 cggataaatg tcgcagtgaa tacgtgatcg aagtgactgg ggaagttaga agacgcggag
     7261 aaaatgcagt aaatcctaaa atggccactg gtgaattcga agtgattgca agtgaaatcg
     7321 tgattttgaa tcaagcaaaa accccgccat ttcaaattga agatgatcaa gcaatcagtg
     7381 acgaaatacg tttgaaatat cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca
     7441 tcaaattaag aaatgcgaca acaaaagcga ttcgtcatta tttagatgat aatgactttc
     7501 tcgatatcga aacaccttac ctagggaaat ctacaccaga aggcgcaaga gactacctag
     7561 ttccttcacg tgtccatgct ggacatttct atgcgttgcc gcaatctcct caattgttta
     7621 aacaattgct gatgaatgct ggattcgacc gttattatca aatcgttcgt tgtttccgcg
     7681 atgaagattt acgtggagac cgtcagcctg aatttacgca agtagatatt gaaacaacgt
     7741 tcttaacagc agaagaaatc caaacttaca cagagggatt gattgcgaag gtaatgaaag
     7801 aagttcgcgg tatcgaagtc acattgccat ttcctcgtat gacctatgat gaagcaatgg
     7861 ctcgctacgg cagcgacaaa ccggatacac gttttgatat ggaattgatc gatctaagcg
     7921 atacagtcaa agaagtagaa ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg
     7981 tgaaagcatt gaatgctaaa ggcgctgcag atcgctactc acgaaaagac atggatcagt
     8041 taggtcaata tgtaggacaa ttcggagcta aggggcttgc atggctgaaa gtagaagaag
     8101 acggattgaa aggtccaatt gctaaattca tgggagaagc aacagaagca atcattaaag
     8161 caacagatgc aaaaccaggt gacttgctga tgtttggcgc agataaaagt gaaatcgtcg
     8221 ctgctgcatt aggtgctatc cggacacgtt taggaaaaga attaggacta attgatgaat
     8281 cgaaattcaa tttcttatgg gtaactgatt ggccacaatt tgaattttca gaagaagaag
     8341 gtcgatatgt ctctgctcac catccattca cgatgccaaa agaagaagat atcccgttat
     8401 tggctgaaga ccctgcaaaa gtctatgcag aagcttatga tatcgtcttg aacggttatg
     8461 aactaggcgg aggttctatc cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa
     8521 ctctaggctt tacaaaagaa caaatggaag aacaatttgg tttcttgtta acagctttag
     8581 attacggatt ccctccacat ggcggtattg cattaggatt ggaccgtttt gcaatgttat
     8641 tagccggaga agataatatt cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag
     8701 atgtgatgag tgatgcacca agtatggttt cgcctttaca attgttcgaa ttgaatattg
     8761 atgtaacgaa tatagaagaa tagccgataa aaaggtaaaa aggatttgtc gaatacgctt
     8821 cttttgacaa ccttgattta attgaaaaac actttataat gaagccataa catataaagc
     8881 ggggcttttc catgaaaaaa acattcgaaa attgggctta tcatgattac acgaccaagc
     8941 tttcggtatc tatcgtttat gcgttgatcg catcagtcgc attgaatttc ttttatcagc
     9001 caggacatat ttattcaagt ggtattactg gtctagctca gattttgacg actttgtcaa
     9061 aatcactgat aggattcgaa gtaccggtat caacaacttt gtatttactg aatatacctt
     9121 tgtttgtatt ggcgtggtta aaaatcggga agaagtttac aattttcact tttatcacag
     9181 tcactttgac ctccctttgt atccatattt taccggaaac agtcctatct accgatccaa
     9241 tcatctgtgc gttgtttggg ggggctgtga tgggactagg tattgggcta gcgttgagaa
     9301 acggcctatc atcgggcggt ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa
     9361 agtcgattgg ttctatttcg atctacttca atgcattgat cgtttttgtt gccggaattc
     9421 tatttggctg gcaatatatg ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa
     9481 ctgatgtcgt ttatactaaa caaaagaaga tgcaggtaat gatcgtaacg aaacatccag
     9541 aaaaagtagt aaatgaaatc caaaactgta tgcgtagagg gatcacgatc atcaatgaag
     9601 cagaaggtgc ttataagcat gataagcaga cagtattgat gacgatcgtt acacgatacg
     9661 aactgcctct acttagagaa gcaatgaaag cttctgatcc acaagctttt gtaagtattg
     9721 cagaaaacgt ccagatactt ggtcgtttct atgaggatgg catttagatc gagtttgaca
     9781 aaaattcatg aaatactgct gtagacagac gttcgtttgt ctgcagtttt tttctaccat
     9841 agtagaatca gcaaagaaaa actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa
     9901 gcatgtttat ttttttaaat ttatgggatg atagaaaaaa atagtagaga aaggacacga
     9961 ttctacttgc tattttcact taatacccgt atattacgaa tgaaaaaagt ttatgaaaag
    10021 ttggaatatt gaatggaaaa acaaaaaagg ttaaaaaaca gccgctacat cactgggttt
    10081 gatgggctcc gttcattagc tgtggtgggt gttatcttct atcaccttct gccaactagt
    10141 atgaaaggcg gctatttggg cgttccgatt tttttcgtcg tatctggtta tttgatcacg
    10201 gatctgctgc ggcaggaatg ggaacaaaat ggaaaaatca atatttggca attttatgtc
    10261 agaagaatga agagactcta tccggcactt gtttttatgc ttgtgacagc ttcggcttat
    10321 atcactttgt ttcagcgagg gctgttaaac aatcttcgag gagttgtggt cagcagctta
    10381 ctgtatgtca acaactggtg gcaaatcaat aacggtttat cttatttcga tcgatttgct
    10441 aatgagtcac cgtttacgca tatctggtct ttagcagtag aaggtcaaaa ctatttggtt
    10501 tggccgatct tgtttgtatt gttgatggta tttgttcgta aaagaaaatg gatcttttat
    10561 actgtgttag gtacatcctt cctctccgcc atcttgatga tgatcttgta tacaccagga
    10621 ggcgatccaa cccgtgttta ttacggtacc gacactcgtc tgttttctat atggttggga
    10681 agtgcgcttg cgtttgtttg gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg
    10741 aaaaatattc tgaatatcgc tggaggaatc tcgcttttgg cactgattct ttgcttcttc
    10801 tttttagatg atcattacaa tttcatctat tacggaggaa tgctcctagt gagtctgtta
    10861 tgcgtagtgc tagttgcagt gacagcacac cccggagcaa gtttgaatcg atggctgaca
    10921 aatccagtat ttacttggat tggcaaacga agctatggca tttatctgta tcagtttcca
    10981 gttatgattt tttatgaagc aaaggtgact gatattgccg atcatgtttt gttgcatact
    11041 ttagtggaaa tcacattgat tctaatcatc agtgaacttt cttatcgata tgtggagcgt
    11101 ccgctagcac gttttgatta tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa
    11161 ccgatcatca gtaagagcaa accatgggtt gttccaggca cattacttgt agcaattgca
    11221 ctcgttggtt tcgttacggc accgaaaaac gctgtaacag ccgatcaaaa acaactgcag
    11281 gcaaaaatcg ctgaaagtaa aaaattagca gaagaaacca gaaacaattc agaaaacacc
    11341 actagttcgg ttgaccaagc gatccttgat aagtacgagt tgtccaaggc acaagggaat
    11401 aaggcacaaa aattagaact aacggcattt ggtgattcag ttatgttgga tgctgcaacg
    11461 gatctacaag aactttatcc aaaagtagtg gtagatggag atgtcggacg acaattatat
    11521 gccagtgtac cgtacattga aaaattaaag gaacaagatt tgttgaaaga cactgttctg
    11581 atcggactag gaacgaatgg ctcattctcg gaagctcagt ttgatagtgt gatggatgca
    11641 atcggtgatc gaaaagtata ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat
    11701 gacgtgaatg cgatgcttga acagatgacg aaaaaatacg acaatatgac gctgattgat
    11761 tggtacagct acagtaatga ccatagtgac tggttttatg atgatcaagt ccatccaaat
    11821 cctgaaggga tgacacatta cattcactta gtcagcgaag ccttgctagg aggaagttca
    11881 agtacgaaca gcagtgagac aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa
    11941 aacatttgtt tttgcctgaa gtttttttat atttaccgtg tttttaacat tcatatagat
    12001 tcaggaagca aaacggttga gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt
    12061 tttttgaatg catcttctgc ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg
    12121 atcatcttaa caggtcttct tttttgaaga cgagtatatt tagcaccagt accattgtta
    12181 tgttcctcta atcgtctaga aagatcggtt gtatatccac cataaaatgt ctgatcttta
    12241 cagaataata cgtaaaaata atgttcattt gccatagagc atctcacgaa tctctgcagt
    12301 gtattctcca tccatattgt aagtgatcaa tggtggagcg actttgaatc cgtctaattt
    12361 gccttctttg attccttcaa ctaacaaaat atttgcctct tttccaggtt ttgggtaaac
    12421 aaattggatc cttttaggtg caattcgata gtcttccatg acctgaatga tttctaagaa
    12481 ccgatcagga cgatggacca tagtaaagcg tccatttgtt tttagtagct tacttgaaat
    12541 atcgatcact tcttctaacg tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt
    12601 agggttcgtt ttatttgttg gcagcccttt gaaataaggt ggattacaga cgactaagtc
    12661 acaagaatta tgtcgaacga cagataagct gtctttcaga tcgatggtgt ggacagtcat
    12721 ttgttcattt aaatggttaa gcgcgatact acgtcttgcc atgtctgcta gacgaggttg
    12781 aagttcgata gcgtcgatag ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc
    12841 atttcctgca caaaggtcaa caatcttccc ttttttaggt acacgggaaa agtgagcaag
    12901 cagcacagca tctaaagaaa aagaaaacac tttggaactt tggataatct tgatatcttc
    12961 tgcgtataat tggtcgattc gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa
    13021 aaatagtcgt ctgtattata ctatttttat taaaaagaac caaggtggct tgtcaaacct
    13081 ctatttttta tgcatactat gaccataaga agggaaagga agagactatg ttttatcgat
    13141 ttatgcgcgg ggtagttcga gtactattag ccttagttaa tggaaatgcc cgctatgaga
    13201 ataaagaagt tttaccgcaa gatgagaatt acatactagt tgctccccat cgtacttggt
    13261 gggacccatt atatctggcg gtagctgcag cacccaagga attcagcttt atggcaaaag
    13321 aagaactgtt caaaaatcca gttctgcgat ttatattaac tcgatccaat gcttttccag
    13381 ttaaaagaga aaatccagga ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga
    13441 caaatttgag tttgatcatg tttcccagtg ggacaagaca tgctacagaa cttaaaggcg
    13501 gaatggcatt gatttccaga atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc
    13561 cgttgacact tggggatctg ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa
    13621 tcgacttatc agatatcaag aaaacggata aagaaggtat ggcggagatc gaacgacgaa
    13681 cacaagcagc ttttgacaaa ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca
    13741 aaaaaaaata aaatgataag tttatgcgtg ggcggtcttt tgaatctact gtcctgttca
    13801 gttcagactt tttcaatgaa aacgagggag agacgtcaag tctgccctct ttttgcgttc
    13861 caatatgtaa aatagaagac aggtgtcatg taatggatca aagtgttata ataattagaa
    13921 ggacattttt tgaggtgatt gaatgcgttt tttacataca gccgattggc atatcggaaa
    13981 aaaactacaa ggttatgact tgattgaaga ccagcgtcat gtactatctt ctatactaga
    14041 tatcgcgaaa gaagaaaaag tagaggcaat cgtgattgcc ggagacttat atgataggag
    14101 tgtcccagct gttgatgccg tagaattata caatgaattg atggtcgatt ggaatttaaa
    14161 ggaaaagatc ccgatttttg ctatatcagg aaatcacgat agcagtacga gactttcagc
    14221 gggtacgcca tggttcagtt attcgaacta ttttttgcga acacagctag cagaagcatt
    14281 tgaaccagtc gagtttaaaa acgtacaatt tttctttctt ccttactttg aaccaattgc
    14341 agcgagatta tattttgaag atgacaatat ccgaacgatc aagcaagcgg tagccaaggt
    14401 gatagataaa atgcaggagc tatttgattc agaaaaaaaa caagtattga ttagccattt
    14461 ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa atcgaagtag gcgggttgga
    14521 ttcgatccct ggtgaactac ttacaccgtt tgattatgta gcactaggac atttgcataa
    14581 tcaagctgct ttgagacagg ataatgctcg ctatagcggt tccccattga aattctcttt
    14641 atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac ctgacaacag gcctggagtt
    14701 gaattttagg gagatcgaac ccctgcatga tattgaagaa ataacagcga gtttcaaaga
    14761 attgctggat cctgcctttt atcagtccgt caatcgggag aattacatct atatctatct
    14821 cactgataga acgatcatcc cgaatatgat gaaccagctt cggcaaatat atcctcgtat
    14881 tttggggatt gaacgggcaa acggtcgagc agaacaaaca aagcgcaaac gaaaagtact
    14941 agaaatgaaa gatcctcaaa aattagcaga agcatttttt agtgaaatga cggaagagca
    15001 gctaactgca aaccaagaaa aatggctcgg agaaacgttg caagaaatca tggaaatgga
    15061 atgatcaaga atgaaaccga aaaaattaca acttaaaaat tttggtcctt ttatcaatga
    15121 gaccgtagat ttttcacgtt tgacagaagc accgcttttt ttgatcagcg ggaagacagg
    15181 agctggcaaa acgacgattt ttgatgggat caccttcgca ctttttggtg aaacttccgg
    15241 aagacttcgc tctggaaaag agatgcgttc tttgtttgct acaccagacg aagaaacaag
    15301 cgtgactttt tcttttgaac atcagcagat gagctatgaa atcgaacgaa aaccagaaca
    15361 agttttagca aaacgaaaag gaaacggaac aaaaaaacag gcagccaaag taacgttgac
    15421 catttttgat gggaaaggaa aagaaataaa acaaatcaca aaacgtacag aagcagatca
    15481 gttgatcaga gaactgatga atctcgatgc gaaacagttt tctcaaatcg ttttactacc
    15541 ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt gataaagaga ctgttttacg
    15601 tcatttattc ggtactcaat ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa
    15661 acaacagcaa caaaaattgg atcatttaga gcaagagctg catcttctgc aaaaaaggtt
    15721 tgttttagtc aaagaagagg aaccagcagc agggtttgaa gccgttttag cccaatggac
    15781 aaaccatcag gcaatactcg aaaaacaaat cacggaagaa aaagaagctc taacggaact
    15841 tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag
    15901 ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa ttagctgctc aaaaagacga
    15961 aatagaggaa aagaaacgat ggattgggta ttatgaattt acgcaacgat tagtcgaacc
    16021 aatccagcgc ctacaagaaa taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga
    16081 gcaaacaaag aaactagcga tgactacgca gaattatgaa gagtggcaaa aaaatgaagc
    16141 aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct caaaaactgc aaaacaaaca
    16201 gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa caagacgcag aacaattaac
    16261 aatttcattg gataaagaag aattgaatgc cgagaagctg tatcaaaatc tcgctatcta
    16321 tcaggaaaga actcgaattc ttcaagaaaa tttactggat caggaaaaac tgcaagaaga
    16381 tcgtcttgct tttggacagt taaaatatct agatgaaaag cagcaagaaa tagaaaaaca
    16441 aaaagcaaaa gacactgggt cgctcgcaca gataagagaa gaactcaaaa aagcaactga
    16501 agaaataaca aaaaatagtc agctattgaa agaacagaca gaacaagcag aacagctgaa
    16561 aagccaatgg gcaaagcagc aaattgctcg tcttcagctt tttttgcgtc caggggagcc
    16621 atgtcttgtt tgtggatcaa cggaacaccc catacaacaa ttaaaacacg aacagccaaa
    16681 taaggaagat atcttaaaag tagaacaaca actggcagaa gctgagtctg ctgtagaaag
    16741 aactcagcaa atcattgcca aaagtgaagc acatgaaaat aatcttcaac aacaattatc
    16801 agatttagaa caaaaaatca tggcaacgaa tatacaattg gctgaacaag aagaacactt
    16861 gcaacaggct ttgaaaaagg catttcctga gataacagaa caaaaaacaa aaacaactct
    16921 tgtagtaatc gaacaatctt tagatagacg aaaaagcaag atggccaatg caagaataga
    16981 gctggaagaa ataagacaac aagcagaaaa gctcgaacaa caattagctg tctacaaaca
    17041 gcagcagaca gaaaaaaata atcagctcca gcaactgcaa ggagaaatca aaagtttaga
    17101 acaacaattg caagatacgg atagtaactg tttagaagag gagatacgac gacttgaaaa
    17161 acagttagaa gaagactcga atcaactagc tgttgagcag aaagcaggag tgcaattgtt
    17221 gcaagatctt catacgcttc ggacacagct tgatcttcaa catgaacaaa tagaaaaaat
    17281 agatgaaaaa atagaaaagg aacaagaaag aatagctaaa aagttagaaa gccagtcgta
    17341 tttttcaaca attgaagaaa tctgtgcatt tgcacaacag caagcacaat ataaagaaaa
    17401 ccaacgtaca gttacttctt atcatgaaga gcaattagtg acagctgacc ggctgcgtca
    17461 attagaaaat atccagctaa ctgagacatt cccagataca gaagagatca gccaacagtt
    17521 agaagaaata agacgtatga tcgaaataaa gcaagaaaag atatacggtt tacaagaaca
    17581 aaaaattagt aatcaaaaaa tctacgatga atgtctttcg atttatcaca caagccagac
    17641 gaaattagat gagcttacgc aattacagca gctttcgcaa acattcaacg gagaaaatcc
    17701 taagaagact agtttggaac ggtatgtgct tcaggtttat cttcaagaag tgttacaagt
    17761 agcaaatgat catctccaac gtttaacgaa aaatcgttat caatttgagc tagcggatac
    17821 cattggcagt tatcgaggga aaaccggatt agaaatcaat atctatgatg atcaggcagg
    17881 tatgtctcga agtgcgcata ccctgtctgg cggtgagagt tttattgcag cactttcttt
    17941 agcattagct cttgccgatg tgatccagac acaagctggt ggtgtagcaa tcgatgcatt
    18001 gtttattgat gaaggatttg ggtcattaga tgaagaagct ttggaaatgg caatggaagc
    18061 tttagaaacg atcgaaagtg aaggaagaat gatcggcatc attagtcatg taagagaatt
    18121 gaaagaacga gtattacaac aaataagaat tgatacagaa ggtagcggac aaagcaaaat
    18181 acgctatcaa cttggataaa aagggagatt ggtaactatg aaatggagta ttccagaaaa
    18241 agtcattgca cgtggacgtc aatacttgca agaaggccga gtgttatcgg ttgtccctga
    18301 ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt gaattgtata tggttgattt
    18361 ggatggtaca gcaaaagaga tagattactg ccaatgtccc tattgggagg aacataaata
    18421 ttgtaagcat acagttgctg tggaacttta tttacggtcg aaaaatctat ctcgtatcat
    18481 gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca agtgagggag aaatcattgc
    18541 aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa gcggtgcaac ctttgattat
    18601 tgaataccaa gtagaaacga ttgagactaa tcagtatcac ccagagcttt ctattttggc
    18661 actttacttg cgtatcggtt cattggcatc accgaaaaaa acatatatcg taaaaaacat
    18721 ctatgatttt ttacaagctt acacagaaaa agaaacttac actgttaata aacagtacca
    18781 gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc
    18841 aggaagtgct cagacgcatc aattattagg tacgaacggc ttacaagtca aaggaaaact
    18901 agataaaaga tatctgcttc tcccagtaga acaagcccgc ttcctgttag agcagatgaa
    18961 tcagactaca cgtttgcatc tgcagataaa tgaacaaaac gatcaaggaa tacaattttc
    19021 cgatcaaaac aaacctttat cattcaaagt ggaaaaattt ggagaagatt atttgctgca
    19081 agcattgaat gattttgatt ttttctttat gcattatcag tggggagtta tccaagggac
    19141 attttattct ctgacgaaat atcaacaaac ggtttatcaa acttggaaac aattgattcg
    19201 ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga
    19261 gattatccct ctgctgtcag agatcggcga cgtagctatt gcccctgcag tagaagagga
    19321 agtagcagat atccccgtcg aatttatttt ctttttccga aaagcaaaag gaaaaatcca
    19381 agctcgagtc gatttcgtct atggcgaagt catctattcg actgacccga agcatgaggt
    19441 ctcgacagaa caggcttttc aggtgttacg tgacacagta gaagagaaac gagtacttga
    19501 tctattcaga atgtatcgct accagaaaaa tgaaactggt tatgaacgtc cattgccaag
    19561 cggggaagaa ctatatgcct ttttccgcac agaactatct gtcttccgtc agtttggtga
    19621 agtcagactt ggaagaaaat tgcgagagtt gtatttagat gcacataaac atcaaccgaa
    19681 aatcgaagta accgagaccg gttcatggct cgatattcga ttcgatgtga cagggataga
    19741 ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga aatgatgctt tttatacatt
    19801 agaaaatggt caggttcttt cttttgattc agaagaattt caacaaacaa gccaagtgtt
    19861 gcaacaatta cgggaatcga tacgtacaga agaaggaacg atccatgtac ctaaaaatca
    19921 aggattgatc attcaaaatc aattggaaaa aagcaatgct acttttagtg aatcgttcca
    19981 aacgatggta caagatctca ttcatccaga aagataccag gcacagcttc ccaaaggatt
    20041 gaatgcaacg atgagagatt atcaaaagca aggttttcga tggttgaaga tgttaggtca
    20101 ttatcagttt ggcggtattt tagccgatga aatgggactt gggaaaacac tacagacaat
    20161 tgccttttta ctttcggaaa aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc
    20221 tgcgagctta atttacaact ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat
    20281 ccaagtaatc aatgggaata aaaaagaacg tgaagaatta ctggctaaag acacagatat
    20341 ccgagtgact tcttatgcca gcttaagaca ggatctagct aattatcagt cgcaaaaaat
    20401 tgattatttg attcttgatg aggcacaaat ggtcaaaaat agcagtacga aaactgcaca
    20461 agcattgaga gaattagctg tacctcagcg gtttgcactg agtgggacac caatcgagaa
    20521 taatttagaa gaattgtggt cattgtttgc aacgattatg ccaggatttt tcccgactaa
    20581 aacaaaattc agggaacttg ccgcagaaga aattgcgcaa atgattcgac cgtttatttt
    20641 acggcgagat aaacaaactg tgttgaaaga cttgccggaa aaaacagaga tgaatctata
    20701 ttctgcactt acagaagaac aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga
    20761 agaaatcaca tcaatggatt ctgaagcatt caagaaaaac cggatcggga tacttgctgg
    20821 tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg tttattgaag attaccaagg
    20881 cggttccggc aagctcgaac aagtaaaaga tctactggta gctgccaaag agaacaaacg
    20941 aagagttttg ctgttttctc aatttacagg gatgctgact atcttgcaag aagagttagc
    21001 cgagctaggg atctcgacat tttatttaag aggcagtacg aaaccacaag atcggttaag
    21061 tatggtagac gcatttaatg ctggcgaaaa agatgttttt ctgatttcct taaaggctgg
    21121 gggaactgga ttgaatctca cgggagcaga tacggtcatc ttgtatgacc tttggtggaa
    21181 tcctgccatt gaagaacaag ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt
    21241 agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa cgcatggatt cactccagca
    21301 agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact
    21361 gactgaagaa gacatccgca tgattttaag tgtaggagaa atcgaggagt aagagactcg
    21421 aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga agcgattgtt tatttgattt
    21481 aaaaaatagc aaggaggctg ggattccatt ttatcccagc ttccttgcta tcattttttt
    21541 atttctattt ttaaaggtta tacaaagatt gtcttcattg tctctaacag ctgatcaaaa
    21601 gaagaaatct cgatatcgca aattggagtc tgttcaattt tgcgttctcg atggttgaac
    21661 caaagcgctt gccagttagc actttttgca cctagaacat cattgtcgta attatcgcca
    21721 acgtataagg tctcttctgg aagcatatga aattctttct cagctagctg aaaaatctct
    21781 ttttctggtt tttggaaacc tgttgcttga gaaatgagca tattgcggga aggaatccaa
    21841 ttgttcaatt gaagttgctt cagttttttt gtctgatggt cagtcggccc atttgtgata
    21901 attcctaatg ggacattctt ttccttcaag aaatcgaggg tttttttgac ctcctcatgc
    21961 aagcagatat tatcaagttc ttcctcgtaa attttttgaa ataagaggcc atcttcttcc
    22021 gtaatatgag ggtagtctaa atcttttagt gattggctga tccgatgtgc tcgcatatat
    22081 tctagtgtcc aatctcctgc cattactttt gggaagtttt catcactatg atggcggaac
    22141 cggatatata gtggatgcat atctgcatct gaaacaagcg gaacgactct tttgaccgcg
    22201 tttcgaaatg gctgctgctg atcatacatc gtatcatcaa catcaaacac aactgattta
    22261 atcaaaattt tacacgcctt tcggttttca ttgatttttt cacaaatcct tgactattgt
    22321 aacagaaaaa attgtctaaa aaagctgata aataaaggtt tttcttacta tatttctatt
    22381 tccttgacag aataaggttt ttatatttca gtttttttca ctaaaaatcg tgaaacacgt
    22441 caataagttt gatgaacaaa ttaatcatta tggtagaagt cgattgattt attttaatgc
    22501 aatctctgtg gaattttcga aagat
//
LOCUS       DAXWLH010000035        22388 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXWLH010000035 DAXWLH010000000
VERSION     DAXWLH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.035"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(12..380)
                     /locus_tag="ACGE36_000970"
     CDS             complement(12..380)
                     /locus_tag="ACGE36_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFF8383386.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            551..1507
                     /locus_tag="ACGE36_000971"
     CDS             551..1507
                     /locus_tag="ACGE36_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8383387.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1580..1696
                     /locus_tag="ACGE36_000972"
     CDS             1580..1696
                     /locus_tag="ACGE36_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFF8383388.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1751..3433)
                     /gene="recN"
                     /locus_tag="ACGE36_000973"
     CDS             complement(1751..3433)
                     /gene="recN"
                     /locus_tag="ACGE36_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFF8383389.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3451..3900)
                     /locus_tag="ACGE36_000974"
     CDS             complement(3451..3900)
                     /locus_tag="ACGE36_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8383390.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(4007..4825)
                     /locus_tag="ACGE36_000975"
     CDS             complement(4007..4825)
                     /locus_tag="ACGE36_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFF8383391.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVASNAHEQFDHK"
     gene            complement(4834..5724)
                     /locus_tag="ACGE36_000976"
     CDS             complement(4834..5724)
                     /locus_tag="ACGE36_000976"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8383392.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5726..5950)
                     /locus_tag="ACGE36_000977"
     CDS             complement(5726..5950)
                     /locus_tag="ACGE36_000977"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFF8383393.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5955..7292)
                     /gene="xseA"
                     /locus_tag="ACGE36_000978"
     CDS             complement(5955..7292)
                     /gene="xseA"
                     /locus_tag="ACGE36_000978"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFF8383394.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7293..8147)
                     /locus_tag="ACGE36_000979"
     CDS             complement(7293..8147)
                     /locus_tag="ACGE36_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFF8383395.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8244..8693)
                     /gene="nusB"
                     /locus_tag="ACGE36_000980"
     CDS             complement(8244..8693)
                     /gene="nusB"
                     /locus_tag="ACGE36_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFF8383396.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8686..9111)
                     /locus_tag="ACGE36_000981"
     CDS             complement(8686..9111)
                     /locus_tag="ACGE36_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8383397.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9357..10421)
                     /locus_tag="ACGE36_000982"
     CDS             complement(9357..10421)
                     /locus_tag="ACGE36_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8383398.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10593..10838)
                     /locus_tag="ACGE36_000983"
     CDS             complement(10593..10838)
                     /locus_tag="ACGE36_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8383399.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10977..11351)
                     /locus_tag="ACGE36_000984"
     CDS             complement(10977..11351)
                     /locus_tag="ACGE36_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFF8383400.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11434..12348)
                     /locus_tag="ACGE36_000985"
     CDS             complement(11434..12348)
                     /locus_tag="ACGE36_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8383401.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12369..13172)
                     /locus_tag="ACGE36_000986"
     CDS             complement(12369..13172)
                     /locus_tag="ACGE36_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFF8383402.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13205..14194)
                     /locus_tag="ACGE36_000987"
     CDS             complement(13205..14194)
                     /locus_tag="ACGE36_000987"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8383403.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14297..14788)
                     /locus_tag="ACGE36_000988"
     CDS             complement(14297..14788)
                     /locus_tag="ACGE36_000988"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8383404.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14979..17786)
                     /locus_tag="ACGE36_000989"
     CDS             complement(14979..17786)
                     /locus_tag="ACGE36_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8383405.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18220..18513)
                     /gene="rpmA"
                     /locus_tag="ACGE36_000990"
     CDS             complement(18220..18513)
                     /gene="rpmA"
                     /locus_tag="ACGE36_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFF8383406.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18526..18870)
                     /locus_tag="ACGE36_000991"
     CDS             complement(18526..18870)
                     /locus_tag="ACGE36_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFF8383407.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18897..19205)
                     /gene="rplU"
                     /locus_tag="ACGE36_000992"
     CDS             complement(18897..19205)
                     /gene="rplU"
                     /locus_tag="ACGE36_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFF8383408.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(19225..19301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19487..20128
                     /locus_tag="ACGE36_000993"
     CDS             19487..20128
                     /locus_tag="ACGE36_000993"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8383409.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20190..21821)
                     /locus_tag="ACGE36_000994"
     CDS             complement(20190..21821)
                     /locus_tag="ACGE36_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFF8383410.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21944..22303)
                     /locus_tag="ACGE36_000995"
     CDS             complement(21944..22303)
                     /locus_tag="ACGE36_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFF8383411.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT         6518 a         4922 c         3715 g         7233 t
ORIGIN      
        1 ttctattttt ttcataggta agaaagtatg ttcacaatga tcaagacagc gtacaagacg
       61 attgtcaaca gaaaaaccaa acgccaagtc atcttgaaat aacgcggata aataatttct
      121 tcataataat atgcttggaa aacagcaata caaattccca ttagcaaaac agaaataaag
      181 aaaaacggta agatagaaat tgtccctgta tttctagaca atgcatgcat accgccccac
      241 aaaaaaggaa tcgccaaatc aggggctttt attttaaaac gacgatttaa cgaaaataat
      301 tttacaagaa attggcaacc aaacaaaacg attaccgggt aaatatacca aaacaagata
      361 agtggtgtga atgtgcccat gaacgtaaac tcctttttta tcgttgcttc aagtatactg
      421 tatatacttt acacaactgt ttcattttat ttaaatttat gcagttatat tctatatctt
      481 acaaacacat tccatgtatt ccaattccct atccgttata ataaaagaaa caggcaacag
      541 gaggaaaatc atgatcaaat attttacatt agataatgag aaactgcgta ataccggaaa
      601 ggaaactgac caaacgatct ggtattgtgt agaacgtcct aatcaagaag agattcatca
      661 gctgacacat caatttgaaa ttccgtcaga ttacatcact tccatattgg acgatgcaga
      721 aaattcccgt acagaagaat tcaatcaatc aaagttgaca aagccagcgt tattattgtt
      781 acaatatcct tttgctaaaa caagtcctag cggttatttt caagtggata catacccgct
      841 ctctattatt ttgacgccca aaaagaagct tatcacaatt acgaatcatg aaccactgtt
      901 tttaaagaaa gtctttagcc agacctttcc aaaaagtgat atttcgccta atctcaatat
      961 cttctttcaa ttcatttggc aggtcacact ctcttacaac agttatttag ctacgcttca
     1021 atctcaatgc gatactttgg aagccaaact tcaagtatcc actgaaaaca aacaacttta
     1081 tcaaatcatg gatatccaaa aaagcctcgt ttatttcaga gaagcgactt ctgctaattt
     1141 agatgcattg aaagaactag cgcaaaataa acttttccaa gaaaatcatg ctttgaaaaa
     1201 tcatttacgt gatgtattgg tggaaacaga gcaggcaatg actacttctc gcattcaatt
     1261 aaaactagta gaacaaatga atcagacttt ttcagcaatc gtatcgaata atctgaataa
     1321 tattatgaaa attttaactt ctttaacgat tattttaact ataccgacaa tcgtcggcag
     1381 tatctatgga atgaacatta agttacctat tgctgaaagg gatgatgcct ttatatggct
     1441 agttctatta acattattta tttgtatcgt aactacctat tatttaaaaa aagggaagtt
     1501 tttataaaaa agccctcttg tggaaatgga aaaaagattg tacacttgaa tcaactaatt
     1561 aatgacgagg ttgaaaacaa tgcgtgaaaa aaaacaaaca agtacttttg ctaaagtaac
     1621 aaaatttgtc atttggacaa tgctgatttt aacgattgga tctgttgtat tgactgctgt
     1681 aatgagtgtc atgtaaaaat aaaatatagc tatcagctgc tgaaaaggac ctgaaccact
     1741 gcgaggtggc tcaggtcctt tttgcgagtt tcaatagttc ttttgcatgt tcgagtgtga
     1801 gttttgtaat ctcacttcca gccagcatac gtgcaatttc ttcttctctt tcttttgttt
     1861 tcaactctga aacagaagtt tgcgtacgtc catcaatcac tgctttcaca atataatatt
     1921 gatagtcggc gactgcagca acttgaggca gatgagtgat gcacaaaact tgagagtatt
     1981 tcgatatttg agaaatttta tctgcgatgg cctgtgcaac tcttccgctc acacctgtat
     2041 ccacttcatc aaaaataata cttgtcacac cttgctcaga agaaaaaata gttttcaaag
     2101 caagcaacat ccgagacagt tcgccaccag aagcgacttt caccaaaggc ttcagtggct
     2161 ctcctggatt ggtcgtaatg taaaattcga tttggtcaaa tccatcatga ttcaattgtc
     2221 tattttcttc ttttaagaaa cgtacctcaa actcagtgtt ttccatatac agacttttca
     2281 attcatgcag aatggatttt tccaattctt ttgcgatttt tttacgcgct tcatgaagat
     2341 ctgcggcttg ttgctgaagt agaagctctt tttgctctag ttcctctttc attttgtcca
     2401 attgaccttc tgaaaaatca gaagaagcta gctcttcggt gatttcgtca tagtaagcca
     2461 atattgactc aatggattcg ccgtatttac gtttcaactg tcggattaat tctaaacgtt
     2521 gcgtaacttc ttctaaacga ctctcatcta actccaataa atcaatctga cgactcatat
     2581 cgccgattgc atcttgcagc aaatagtaag cactttggat attttcataa attgcttcat
     2641 actccacatc tagatgggcg atcccttgca gttctgaaac ggccaagcca acaccatcta
     2701 cactgttttg ttcattattc gtcagtgctc cgtaacctgc agctaatcca tcagcaattt
     2761 tttgataatt gctaagtttt tctctttctt cttttaattt ctcttcttct ccgacttgca
     2821 aatcagcagc agctatttct tcttgctgga aatggagcat atctattctc tgaacatagg
     2881 atttttcatt ttgctgcact tttcgtaccc ttttttccag ctcacgataa gagatatatt
     2941 cttgctgata actctttttc ttctgttgga aagctacatc tccaaaacga tccaataaag
     3001 caagatgtga ttcaggctgg agcagttcct gatgttcgtt ttgtccttgg atatctacta
     3061 aataactgcc gattcttctc agattagcta atgtgataat atgaccattc actcgacaaa
     3121 cattttttcc agttaaggac atatcccgtc gaacaatcag attgtcctca tctgtttcga
     3181 tccctaattc aaccatcagt tcagagaatc cttcttgttt tggtagttca aacaaccctt
     3241 caagcacaca tttttctgct ccttgacgta tataatcgct tgaacctctt cctcctgcta
     3301 acagacctaa agcatcgata ataatggatt tacccgcacc agtctctccg gtcagcgcgg
     3361 tcattccttc gtgaaaagaa agccgcaatt ccggaataat cgcaaagtta gtaatactga
     3421 tttcttgtag catcacattc gctcctcaac ttaaaggtaa tgcaggaagt ctttcttcaa
     3481 taaaactgcc tggtcttcag ttcgtgtaat catcaaaaca ttatcgtcat cattgataat
     3541 cgaaaataat tctttcttgt aacgtttatc aatcaaattt gctgctgccg aagcatttcc
     3601 cggcaatgtt ttcaaaataa caaatttttc catttggtca actgccacga aagcgtcttt
     3661 taatagtttt tccaatttag tatttacatc ttcgtttgtt tcagctggta tgctataacg
     3721 gtagccgcca tctgaagccg gtaccttgat cagtttcagt tcctttatat ctctggaaat
     3781 cgtagcttga gtaacagata ttcctttgct tttcaggatt tcaacaaatt cttcctgctt
     3841 tcggatatct tgctcactca acaatcgagt aatcaaacga tgccgatctt tttttctcat
     3901 tacgtcttcg ctccttagca tattcttctt aatcaatgat agcttatttc cttggtataa
     3961 taaagaagaa tccgcatact tctcacttga ttttcacagt tacaatttat ttatggtcaa
     4021 actgttcgtg tgcatttgaa gccacttctg ccatattaat agctgaattg attgttccgc
     4081 tttcgggtac tttcctcaag tgagccaaga actcgatatt tccttctccc cccgtgatcg
     4141 gagaataact taattccagc acatcatatc caccttgtgc cgcaaatgac aaaatgtctt
     4201 ctaaaacttt ttgatgggtc tgagggtcac gaacaattcc gtttttccct acagcttctc
     4261 tgcctgcttc gaattggggt ttgattaaag caatcacttc tccctcttct tttaaaatag
     4321 catgtaatgg aggcagcatc agttttagtg aaataaaaga aacatcgata acagcaactt
     4381 ctggaattcc taaggtaaag tcttcgggtt ttgcataacg aaaattcact ctttccatta
     4441 cgacgactct aggatcttgt cttattttcc aagctagctg attgtagcca acgtctaaag
     4501 cataactcat ttttgcgcca ttttgcaaag ctgcatcagt aaatccgcca gtggatgcac
     4561 caatatctaa caatgttttt ccttcaacag atagatcaaa tgctttgagt gctttttcta
     4621 attttaatcc ccctcgagaa acataaggaa gttttttacc tttgactctt aattcactac
     4681 tcactggaat tttttctccc ggtttatcaa accgctcgtt tttttcgttg taaatcaaac
     4741 cagccatcac tgaacgtttg gcttgttctc ttgtttcaaa taatccttgt ttgaccgcta
     4801 atacatctac gcgttctttt tccattcttt ccctcatttc tccacatcaa attgcttgat
     4861 catctggcgc atcagcccgc catcaaaaga aaaaacgtcc tcttctaatt tgtctatgat
     4921 cttgttggcg gataacagct caatctccaa cgcctcacgt gtcttttcta ggcctaataa
     4981 acgcgggtaa gtattttttt ccatacgttc atctttacca gctgttttcc ctaatgtttt
     5041 cgttgtacca atcacatcga gtagatcatc tcttatctga aaagctaaac ctaaatgttc
     5101 ggctaatcgt tgaagtagca ataagatttc ttcaggttgc ttcgctaata tgccaccagc
     5161 cagcaaggca taacgaatca aatgccctgt ctttctttca tgaataaatg ctaattcttc
     5221 taaagaaagc tttttcgatt cgccctcgat atctgcagct tgcccagcaa ccataccttg
     5281 tgatcccgca caaacagcta attgctgaag cagcagaagc ttaggagaat tacctaagtg
     5341 tgccatactg attagctgga atgctcctgt aagcaaacca tctccggcca atatagctaa
     5401 tgcttcgccg aatactttat gattggtagg ttttcctcta cgaagatcat cattgtccat
     5461 cgcaggcaaa tcatcgtgga tcaaggaata cgtatgcacc atttctaaag cagctgctac
     5521 ttgatagacg ggaacatcaa taggctcatc aaaggaagca acggccgtta aaagtaataa
     5581 gggacgtatt cttttcccgc ctgctttcac tgaataactc atcgcatcat agagtcctct
     5641 atccgttgta tgctcatcta aaaaagacaa gatttctttt tctacagcag gtagatgaat
     5701 agctgaaaag tcagttaatc tcatctcagc tttcctcgtt tccgtcaaaa tcaacttctt
     5761 catttgattc tgtcatcatt ttcgtcaagg tctcttctgc tttagctaat gtatcttggc
     5821 aatgtttgct taattccatt cctcgtttaa acgaatctag agctgattct aatggaacgt
     5881 ctccttgttc taactgcatg acaatttttt ctaattcttg cagtgactct tcaaaagttg
     5941 gattttccat atttttattc tcctttgtct atctcttgta cttgtccttt aacgattcca
     6001 tcttgataat ggatcgtcag caaatcccct tgatgaatct cggtgacact ttttactact
     6061 tgatcattta acgtcgtata gctgtagcct cttcccatga ttttcaatgg actcaacaaa
     6121 tcaagagatt gaacagcttg agcaaaacgc tgctgctgtt gtttcatata ttgttggatt
     6181 ttctcttcta agcgtttttc tagataagca gtttcttggc gagctgcttt aactcgataa
     6241 agcggagcca cttgttccaa ttggtggctt aatgcgatcg tttgtttttc tttcgcatga
     6301 taaatttgct gtgtagcttg atataatcgc tgtttcaact ggtctaattt gatggtctga
     6361 ccttcataca atctttcagg ctgccgaaat acataggaac gtcgagcacg ctcgaaccgt
     6421 tcttgtttgc gctggatctg gcgtacaaac gcctgctgca atctagcttg ccgttcttga
     6481 atgcgcatca actcttctga caaaacagga acagctaatt ctgcagctgc tgttggagtg
     6541 gcagcccgaa catctgctac taaatcagcg atcgtggtat ctgtttcatg tcctacagat
     6601 gaaatcaccg gcgtattagc ttgataaatc gcccgagcaa cccgctcttc attaaacggc
     6661 cataaatcct caatcgaacc acccccacga ccgatgatca ttgtatcgaa attgcccatc
     6721 tcttctactc tctggatatt cctgacaaca ttgtccgctg cttggtttcc ttgtacaact
     6781 gttgggaaca aaaccaattg gacaattgga tatcttcttt tcaccgtcgt gatgatatcg
     6841 cggataaccg ctccgcttgg actggtaagg accgcgattc gctttgggaa acgtggcagc
     6901 atttttttag gcccttgaaa aaggccttcc ttcatcagct tttcttttcg ttcttccaat
     6961 tcttgatata aagcacctat tccatcaggc tccatgtgct ctacatagat ttgataagaa
     7021 cctcccgctt catataatga aatacgccca acgacaagga ctttcattcc ttcttttggc
     7081 tggaatcgta atttttggaa tgctcctttg aacataatag cagatatttt tgcatggtca
     7141 tcttttaagc tgaaatattg atgggcattg gcacgtaaac ggaaattcga tatttcgcct
     7201 gttaaatata cgcgttctaa ataaggatcg gcatcaaatt ttcttttcaa atacttcgtt
     7261 aatgtagtga cagttaaata ttcttgtgcc atctattcac ctgctccaaa agcttcggca
     7321 gattttacgg tttgatacat cagcatcgtg atagtcattg gccctacacc ctttggcaca
     7381 ggagtgatcc agcctgcaat aggctccact tcatcaaatt tcacatcacc gatcagctta
     7441 ccgttagcat cacgattcat ccctacatct atcactacag cacctggttt tacgaattct
     7501 tttgtgacaa aatgtccacg tcctattgct acgactaaaa tatctgcttc ttgtgccagt
     7561 tcagccagat tttcggtttt agaatgagca acagtaacag ttgcatctgc catcaagagt
     7621 agctgagcca tcggtttacc gacgatattg cttcgtccga taactactgc tcgtttaccc
     7681 gccggatcga tagaataagc ttcaaacatt ttcatgatgc cgtatggagt acaaggaatc
     7741 ttgtccggtt tacccgcaag gagtctgcct aaattcatcg gatgaaaacc gtctacgtct
     7801 ttttcagggc ggatagctaa caggatcttt tcttcatcta tatgctttgg caatggtaat
     7861 tggaccaata taccatgaaa actaggatct tgattgtatt tttcaatttc tgcaagtagt
     7921 tcgtcttcag aaatcgtctc aggataacgc tctaccaatg aatggattcc gatttctttt
     7981 gctacacgtt ctttatttct tacatagatt tggctggctg gattttcccc taccaataaa
     8041 acgactaatc cgggatggat accattatct ttcaattttt ggattttttc agcaatctct
     8101 gcctgcattt tttcagctaa ttcttttccg ttaatcaatt ctgccatcta accactccca
     8161 gatatttttt gtgttttcat tctaccatag aattttcccg atttataaca ggtttatgat
     8221 gattcataga aaaaagctag gacttacgcc ccagcttcta attctttcaa tacattggat
     8281 aaaacaccgt tgacgaattt acgtgaacgg tcatcactaa atgtttttgc caactcgatt
     8341 gcttcattta aagcaacagc cgctggtaca tctgtcacat aagtcatttc aaaaatcgcg
     8401 atacgcaaga tcaccaaatc catttttgag atccgattga tgtgccaatt gttttttaaa
     8461 tgtttttcaa tcactttatc caattcatct ttttttgcac agacaccgcc aactagcgta
     8521 tcaagataaa caggaacaaa ctctgattca tcttcgttga tcatgtcgcg gtggtctaat
     8581 tcgattgcgt tgaaaatcgc atcttctttt gttaggtctg cattgaagtc taatggaaat
     8641 aaagcctgca atgccatttc ccgaatctcg tgtcttgata attctttatt cattctcttc
     8701 ctcttcgtct tcaaaaagtt cttcgattgt tggttgttct aatttttcag gaacaacagc
     8761 tactacatgg atattgactt cagataaatc gatatccgtc atgaaaagga cttgctggct
     8821 gacccgttcc tgcatttcca ttgctacttt aggaactgaa acaccatatt ctagataaca
     8881 gtaaagatct actttcaagc catcttcctc gtttgctaag taaacacctt ttccgtgagc
     8941 tgcacgtcct aataattcag tgacattgct ggcaaaagtt ccgcgcattc cgtatacgcc
     9001 ttctacttta gaagctgcaa tcccgataat tacttcgatg acttcaggag cgatcacgat
     9061 ctctcctaaa tcttggtttg cgtttaaaat caaatttttt tcatctgcca tttatttatt
     9121 cctccctttg cctaagcacg agatacatcc gaattctcta tgatgcggca tagtgatcac
     9181 gtggtaagaa aactgccgcc gatcagctgc aaactgctat gaattggaga aaacacccaa
     9241 ctttattatc tcttacagct taaaagttct tctatagtgt atcattaaat cgagtgattt
     9301 tctataacaa agtctgtgaa cactgatttt tacgcttact tcttctattg atgaaactat
     9361 aagatgatca attcttttgg tgaacgagtc aacacacgat ttccatcttc tgtgatcaac
     9421 aagtcatcct caatacgtac tccaccgatt cctggtaagt agatccctgg ttcatcagtg
     9481 atcacatttc ctggtacaaa ttgcttttct gcacgagaag aaacgtttgg tccttcgtgg
     9541 atctctaaac cgatcccatg tcctgtagaa tgtccaaatg cttccccgta accatgtttt
     9601 gaaatataat ctctagcaac tgcatctaat tggacaccag tcattcctgg ttttgcagca
     9661 tcaataactg ctaattgagc atctaagact atttgataaa tttcttttaa tttttcccca
     9721 ggatcaccaa tagcgaatgt acgtgtcata tcggaaacgt acccttcgta atagcaacca
     9781 aagtccaatg tgatcaagtc accttgttcg atgatttttt tgcttgctac accatgcggc
     9841 atagccgaac gcaatccgct agcaacgatc gtatcaaaag aaacacctga agcgccaaga
     9901 gaacgcatat aaaagtctag ttgatttgcg acttcaatct cagtcatgcc cggttggatc
     9961 attttcagga tgtgatcata tgccatatcc gcaatatgac atgctttttc gataatagcg
    10021 atttcttctt catctttcac ttctctgagt tcttcgatca tctgtgaaac aggtactaat
    10081 tccgcatcga tgatttcttc taaaaccgaa tattctaaaa atgagactgt tgtttcctca
    10141 aaagctaatg agtgcagttc ttctttgcgt actaagtttg ctacctcatc aaaaataggt
    10201 ccgacatttt taataatttc aaacccttga gcctgcgctg ccgcctgctc ggtataacga
    10261 aaatcagtaa taaaaaatgc tttttccaaa gtaataacag ctaatcctgt cgtacctgta
    10321 aagttcgtta agtaacgaag attgtacgga ctggtaacca aaaatgaatc caaattttct
    10381 tcttgcattt ttttgcgcaa tttttctaca cgtagcatca taatgaaact ccctctttct
    10441 ttgcacgctt ttttccatta tagcaaactt ttcttcgtac acgacagtta aataaaaatt
    10501 tccggaaaac ttctttttat tttaaataaa taaagccaaa aaggagtcac agtctcgctg
    10561 caactccttt tagtttacta ttttgtaaaa acttatgatc gttttccaaa gaaaaatgag
    10621 acaactgcta ccaaaatcac tgcgcctaaa atagatggaa taagtgccat acctgcagct
    10681 tttggtcccc attcgccgaa aatcgcttgt ccaatacttg aaccgactag tccagcaatg
    10741 atattcgtta cacagcccat cgaatttcct ttactggtga tcgcacctgc aattgctccg
    10801 atcacggctc caacaatcaa tgtccataac caactcataa tataacctcc ttctttgcta
    10861 atcaatttta gcatatggga tttaaagggg acaaataatt ggttttgcta tttcatattt
    10921 tcttctgcaa agtaaaaaaa taaagaaaac ctcctcttag aagttttctt tattttttac
    10981 ttgcgtttaa acatactacg aataccacgt ttcactacat caaagaaact caatgattgt
    11041 accattctat cttcatctac atattctctg atcgcacgaa gcactttttt aggttctttg
    11101 gaagaaaatg tgtatgtacc attttttttc gtttgaatgg catatcgagg aatccatttt
    11161 ccctttagca tgactgatgc tagtacataa tccacttctt cccatggaat ttggatatat
    11221 ttgcgtctat ctcgcgcatg gtaaaactca aaaccattat cacctatcat gatttgcccg
    11281 taatctgtca gacccgtaaa cgaagtagca tcaattacca agtctacttt tgtattgatt
    11341 gattgaacca ttttctccct ccagatattt gtttatgaac agtatactat ataaaaaaag
    11401 ctcgggacaa cgcccgagct atatttttat ggattataaa agtccgatga cgtgtccaac
    11461 aacaccgact acgaataaac caaggatgat aacgattgga gaaacctttt tcttaagcaa
    11521 ccacatacat aacaatgtta atcctaatgc agctaaacca ggaatcaatt ggtctaagtt
    11581 gtcttgtaat gttgttacct tttcaggaga aagggctaag ccattattaa cttgttcaaa
    11641 tgcactcttg attccttcac cacctgaagg caatttatcc cattcgatgt aagcaccttt
    11701 atctaatttt acagttgata caactggtaa gaatttgatt gatacccatc tttgtacaag
    11761 ggcagctaat acgaacatcc ctaaaattga agcacctttt gttacgtctt gtaacaatcc
    11821 gccagaaagg tcatcagtaa ttttagaacc agctttgtag ccaaattctt gtgtatacca
    11881 catgaatgcc caacgaataa tgttccaacc taagaagaag atgattggtc caaggatatt
    11941 tcctcccata gctaatgaag cacctagtgc acctagcatt ggacgtactg taaaccagaa
    12001 cactgggtca ccaacaccag ctaaaggacc catcatacct actttaaccc cttgaatcgc
    12061 tacatcatca acaggcgcgc cattcgcacg ttcttcttcc agagccaatg taacccctaa
    12121 aattggagaa gcaatgtatg ggtgtgtatt aaagaattcc aagtgacgtt ttaatgctgc
    12181 agaacgttct tcttttgttt tgtataattt tttgattgct ggaatcatag aaaatgccca
    12241 gccaccgttt tgcatacgtt cgtagttcca tgaaccttga atgaaggtag agcgccacgc
    12301 aacagctaaa cgatcttttt ttgataattt gattttttct tctgccattt tattttcctc
    12361 cttcaagatt aatagtcatt caaaatgtcg cctaaagggt caccagagtt gcttccgcct
    12421 ccattatttg atgaaccacc cattttagaa aggttcaagt aaataagagc taatgcaaca
    12481 cctaaagcac ctagagcaat caatgttaat tgagaaattg cagctacaac gaaaccaata
    12541 acgaagaatg gccatacttc ttttgtagcc atcatgttga ttaccaaagc gtaacctaca
    12601 gctacgacca ttcccccacc gatagccata ccatctgtta accatgctgg cattgattca
    12661 aggaatgatt gcacagtttg tgctgggata aatagcaacg ctgctgctgg gaccgcgata
    12721 cgaacccctt gtaaacatac agcaatgatg tgccacattt caaccttacg gatgtttcct
    12781 tcttcagcag ccgcatccat caaatgaacg attggaacag cgattgtacg tacgatcatt
    12841 gttaagaaca accctgcaac agccaaagga acggcaatcg cgatcgcaga aggaacacct
    12901 ttaacacctt gtccacctaa aactaaaata attgctgatg ctactgatgc taatgcagca
    12961 tctggtgcta cagcggcacc gatatttgcc caacctaaag cgatcatctg aagcgtaccg
    13021 ccaagtacga taccagcttc gaggttaccg gtaactaaac cgatcaatgt acatgccact
    13081 agtggttgat gaaattgaaa ttcatcaaga ataccttcca taccagctag aaaggcaact
    13141 aatacgacta aaattattga aataatagac atgataagcc tcctataaat tttaatatta
    13201 tttattacgc ttttgctaat tcagcttttg cttttttcaa aatatcatcc atgttatcgc
    13261 gtgaatcatt aggtacttta cgtacatcga atttcacacc tttttgttca agcttctcaa
    13321 acgcttcaac gtcttcttgt cccatagaca atactttact tacaacgact ttaccgaccg
    13381 aatgcgccat tgacccaaca tttaattctt tgatgtctac gccaccttca acagctgtca
    13441 atacgtcttc aggattttca aataaaagca atgctttggt atttccaaaa cgagggtctt
    13501 ttgctacttc gatcattttg ctaataggaa taacatttgc ttttacacct ggaggagcag
    13561 cttgttcgat tagtctctta cgcagatcat cctttgatac tgcatctgaa acaacgataa
    13621 tacggtttgg ttgcactgct tttgtccaag ctgtcgcaac ttgtccatgt aacagacgtg
    13681 agtcaacacg tgctaataca tatttgattt ttccgtctcc gaccacagtt ccttctggaa
    13741 gtgctccttt aggttgtgcg tcttcaactg ctgctttcgg tgcttctgcc ggttctaatt
    13801 cttccggttt tacttttaca ccgtcttttg ctgtttcaat gatgtgtgct gcaatttcat
    13861 gagcagattc cattgaaaag cgtgatgcgt aggcttcaat caacattggt aaattcaaac
    13921 cgctgacgat tgcccattta tctttgtgct cttcgaataa tgtgtttgat tgattgaacg
    13981 gtgttccgcc ccataaatca actaagaata acacttcgtc ttgattatca aaagaagcaa
    14041 ccgcttcttc caatttggct ctcaagtcat ctggaccttc acttggtttc aaaatcacgg
    14101 ctttgacgtt ttcttgttcg ccaaagatca ttgaaccgga ttgtaagatt ccttcagcaa
    14161 attgtccatg acttgctagg ataatcccta ccatatttat acctcctatt tacttttgtt
    14221 gtaacaaatt caaaaatatt aataattgct aaccattaca acagaattat ttgacctttt
    14281 ccaacaacca ttcttgctac acctttttga ttttcgctag tagttccatc aaaaggattt
    14341 tcctgtcagc aggtactttt cgaatctcta actccacttc ttgactgttc aagtaacgaa
    14401 aggaatcaat atcttgttga tttactgaaa caaaattggt aatcattgat ttgccttcag
    14461 taaaactcat accgccgata ttcaacgact tcaactgtac accgcagcgg acaaccgctt
    14521 caacatcttg gggattggtg aataaaagca tcacttttgc tcgtgataat aatccactat
    14581 gatagatttg aatcattttc tgcacggtta ttacatttgc tgtgatcccc ggcggtgcag
    14641 cttcttttag tagaaacttt ctcaatttat cttgagcgac tacatcgctg atcacaatga
    14701 tacgttcaac acctgtttgc ttcgaccaaa ctgttgcgac ttgcccgtgg attaaacgat
    14761 catctatacg tgccaatcga atatccatct gcaaaactcc ttcttcctca ctacttcaca
    14821 gaaaccccta tcatttctgt gtgaacaacc acctcctagc acacactact ataagcaaaa
    14881 agcgtgccaa cttttttacg atacactaat tgaatttaaa ggattcttaa ccaatacaca
    14941 aaaacgatac actataaaag tgtatcgttt ttgtgtattc atggcacagt gtattctttt
    15001 ttatctaact gattatggac tagacgtagc agataataca tttcttctat cggcactttt
    15061 acttttatct gatcttccaa cagaacaact gaggctgcca attggctata aagaggatca
    15121 ttcgttgtat ttgtcaattc gttttcttcc acagtcaatg gttcattccg aataatacgt
    15181 tcgatcattc ctgctgtgtg catcactagg ttgattttga atccatattc ttcatcgccg
    15241 attcccgtcg tctgagagat ttgtgtagaa aattgccaca ttggatcgat taacttacta
    15301 ccattaataa agatgaagtt atcagagata aactcaacac acgtattttt agctgactct
    15361 tcatctaggg agatgaattc agtttcctcc aattctgatt ccatcagcaa ctgatccaaa
    15421 atgacttcga tattttgatc aataaaacgt tctaaaggaa taaatggggc ttggatattc
    15481 aaatcagtaa tcccagtagt agctatgatc tgatattctt tttggataac tggcagttct
    15541 tctttcactt ccacaatcga caaagctaga acttctaatt ctgtttcttg acgcttgttt
    15601 accgctcgtt cgatcaattc tttgattctt tgtgctgtac cttcgccaga tgcgcagata
    15661 gccaaaacag cttttttctt atgaataggt tttgcttcct gaacagattc tacagaagat
    15721 atttctgcgt aacctcgaaa atttttcaat gattcatgca acatatctag atctgtccct
    15781 aatactgaga cctttcgtac agtttccaaa actagagaag ttgttaccat atcaacagta
    15841 cgtactggaa ttccagtgtc tctttgaatt tgcgaattaa acgacgcaag ggaccccata
    15901 tctactaaga gtatgacccc gcttccttca tctacttctt gtacattgcg ttcaattcgg
    15961 tccaatgctg ttttcggatc catatccaaa ggcatgtcta ctgctcgtac gttctctgca
    16021 tctaacaact gttggacaac ttggaccata ctgcttgctg tgctgtttcc gtgagctgca
    16081 acgactacac cgattctgcc gcttgcttga tttgcccgta acgagacgag tagaacggtc
    16141 aagtaatagt cttcactttc tggaatctcc acttgaaaat acgtttcgat ataactacgc
    16201 acttctttcg caacagcata ttcatctgga aaatcaaaag ccatctcacg gatgttatca
    16261 ttcgtatgcc gttcttctcc taagttgatt tttttcaaaa aagaactgat atgcaagctc
    16321 atagcataaa taaagttttg ctgaaactca tatggtaaag atcttttgac cattacataa
    16381 atctgattgg tcacttctat gattttcgga tctacaaatt ctgctaattt gctgtctgca
    16441 ttaaaagaaa aaccatggtc tttatagaaa gacttcagat ggatgttgat atctgtagaa
    16501 atgaaatgat tgattgcttc ttgatccaaa ccatccgact tcagtaaagc tgctttatct
    16561 ccgatgatat catatagatt ataaggtaac tcataggagt ccgattggat tttcatgatc
    16621 gtatcatttg gtgctacagt tatctttggc tccaatattt tagaaagctc tgccattgca
    16681 ctgcggttgc ttgctaattg aatcaatccg ctacgaatcc cttctgtcaa atcatcgatt
    16741 gtgatcgaga tttcgggtga ttgcatatga tttagaaatc ctcgtgcaca tactaactgg
    16801 atatttgact tcaattgtcc tatatttcca taggtcacac tgccgatcaa tgcttttgct
    16861 acatcttctg tcaaagaaat ctttcgctgc gtgcggtttg cttcgtgggc aatcatgatt
    16921 tttactaaat cgactttctc atttgcagga cgtttttcaa aagccggaag tttgatattg
    16981 attgggatac gccgaacaaa agtttctaat agagaagaac ctgggtcttc tgtggtcgca
    17041 cctacgatcc gtacatttgc ttcatgggat ttcgttgttt ccccaagacg actgtaagtt
    17101 ccatgatcca taaagtaaaa gatcatttct tgaccttcag gcggcaaccg gtgaatctca
    17161 tccaaaaaca gcataccgcc atccgcttga tcgataatcc ccattttttc ttcatctgcg
    17221 cctgtaaaag caccttttgc gtacccaaat aaatgactca tcaaaagctc agggttattg
    17281 gcataatcag cacaattgaa aacaatcagt tccgtttcct catctattac atgatgcgct
    17341 tttgcaaaat gatacatcgc gtgtgcaaaa taagtctttc ctgatccggt aggtcctgta
    17401 atcaaacaat ttaatcctct tggcggatac aaaatcgcag cttttgcctg ttccactgaa
    17461 tttttcatgc tgccgttagc tccaatgatt tttgcaaaga tatccttagt atctatttgg
    17521 agtgtatttt tttcttcaga gaccggcgtt tctttgtaac tgactacatg ttttgtaact
    17581 ggctttacgt gcgtaacgag tgtttgatat atgtaacgta ctggtcgtcc gtctgtcttt
    17641 atcagttttc cttcgcgcac tagctgattc agatctttac tgctatttgt tcgctgaatc
    17701 cctaacactt cagcaatttc ctgtgttgtt actccttgat catattctgc ctttgttaaa
    17761 tgagcggttt tttctttgac atacatataa attcggtcaa tgcgttttgc caatggtatc
    17821 gctccttttc tatagactat tgtacttagg cttcaaaaca taatactatg tatttatttc
    17881 tgtttcgtca actatttacc tgattttgtt ttgttgataa cacgcggata gatagaaatg
    17941 ttcaatttaa aagaacgaaa aatcccgata cgatttttat aatggaaaca agaaaaagaa
    18001 cactgtttac catataatta tttcactgat agcaaacaat atagcttcca tcaattgaaa
    18061 acgatagtta tcaaaacatc ctgattccaa attggcaaaa aaatattaag tgtaaaaaga
    18121 caaagtaaac ttcgtagcct ccactacaac ttttattttt ataaacaaaa aaggctgtga
    18181 caaaagtctt gtcacaacct ctttaatcat aagattcaat taagcttctt gagctactgg
    18241 ataaacagac acttgttttt tgttacggcc tttacgttcg aaacgtacta cgccatcaac
    18301 ttttgcaaat aaagtgtcgt ctccaccgat acccacgttt acacctggat agatttttgt
    18361 accgcgttga cggtaaagaa ttgatccacc tgtaactgtt tgtccatcag cacgtttagc
    18421 acctagacgt tttgattctg agtcacggcc gttagatgta gaaccgccac cttttttgtg
    18481 ggcaaataat tgtagattca tagataacaa catggtctgc acctcctatt tatcattaat
    18541 tgttttaatt tggatatatt ctgagttttc agcttctatc gattgtaatc ctaataaaag
    18601 attttctaat agaatctcag caatattgtt ttgttcttgt gtcattcctg aagtgacttc
    18661 ggtataaaga taaccgcctt cttcttcatt catttcaaca atcggctcaa aaccggctaa
    18721 tgaggcaatc ccgttgacag tgctgatcgc taacgcagaa acacccgcac aaacgatatc
    18781 acttccataa ggaccagcat cagcatgccc ggtcaaagtg aatgaaacga tctgacctgc
    18841 gtcatttcgc ttaaatgttc ctttgatcat atataggcct tctttctcaa acgctattaa
    18901 gcgttgatcg cgtcgataac cacttttgta tatggttgac gatgaccttg tttacggtgt
    18961 gaatgttttt taggtttgta tttgaaagta acgactttct tttgtttgcc gtgtttttca
    19021 acagtacctt caacagttgc acctgcaaca gttggagcgc ctacttttgt agattctccg
    19081 cctactaaga taacttcgtc aaaaacaact ttttctcctg cttcaacgtc aagtttttca
    19141 acatagattg cttgtcccac ttcaactttt acttgtttac caccagtttt aataattgcg
    19201 tacatgctat aaagcacctc cttctgatat acttagactc gccatccgaa gtgacagcat
    19261 atgctgtgct tattacttgt tctgagcggt tgtagctgtg gagtccacaa ttacaacagt
    19321 actagcttac cagaacagca accgttagtc aactaaattt aaaatcaaaa agtaaaacaa
    19381 aaattcaagc aatacttctg cttttctatc ctcacagttc taccaaataa aatagtcgca
    19441 ttgtgtttct tttattctta taataaaaga aaaaggagga gcataaatgg aatacccaaa
    19501 agaactgata caagaagctt ctttaagaat gaacggccgt cctgtagaag gatttatcgc
    19561 tgggcaagga cctcttcatc ctaaactgat gctcgtggga gaagccccag ggaaaacaga
    19621 gattgatacg catgttcctt ttagcggtca agctggaaaa gaattgatgc aggcactttc
    19681 ttcaactggc ttaacacgag aagaggtgta tatcaccagt gctgtaagga gccgcccgta
    19741 tcgcgtgact catcggatca ataaacggac acaacagtca gaaactgttt atccaaatcg
    19801 gacacctact cgttccgaag tatttgcgca tgcaccaata ttggattacg aactgatgaa
    19861 ggtcccgcca aaattaattg cgacacttgg aaacattggt cttcaacgtt tacttggcaa
    19921 agaatatacg gttaccaaaa atcatggtca actctataca gggcctgtct tacaattaac
    19981 agaacaaaag gatgcctacg aatggtcaga aaaaaactat cgaattcttc cactttttca
    20041 tccagcagct attttctaca atcgctccct tgtacctgtt atccatcaag actggcaagt
    20101 actagcttca cttttacagc aatattaaaa agaacagaca agaccataaa atcttcagtt
    20161 tacagtcttg tctgttcttt ttcattgttt taatggctaa ttgcagtata tttcgcatgt
    20221 aaagctgtca acgcatgagc aactgtttga tgttgatgcg tgaatgtatg ttcccacaag
    20281 tgagaaagtt gtttttctac ttctggctgc aaggaagtat agataatcgt ttgtcctctt
    20341 tgctctgctt tttcttgaaa ttcatcaaaa actgcacgtg ccgtcaaatc aatacttggt
    20401 acgcctcgca tggagaaaat gacaccatcg tgatgattta gttcttccaa tttatttttg
    20461 agtttttctg aagacataaa aaacagtgga ccactaatat acactactgt ccagttattc
    20521 agcttttccg tttctggcag ccccattctt tgccaatcga tcgtttctaa tgaaatcgtg
    20581 atagcagcgc ttttgacaac aaaaaataca cagccgccga tcaccccgat cacgatagca
    20641 atacttaaat caaagataac tgtacaaatc atcgtaagga taaacaatag aactgctgac
    20701 caatatttat tagtaaatag ctccttgatc gtttcccatt cattcatacg ccaagccgtc
    20761 acaatcaaaa cacctgcaag tgctggcatt ggaatctgtg acatcactgg cgcaaaaaca
    20821 agcatggaga gcagtaagac aactgcatgt atcataccag ctaatctagt ttgtgcacca
    20881 gatttgatgg ccacgcttgt acgggcgatg gccgcagtag caggaatccc gccaaagaaa
    20941 ggaagcaaca gatttccaat tccttgcgcc attaattctt gattgctatc taagggctta
    21001 tttgccattc ggccagccga agcaccacat agcaagcttt caatcattcc taataaagca
    21061 atgctaatag ctggaactgc cacttgttgc aaggcagaaa agctaaaatc tcccatattc
    21121 aaacgattac tactgatcaa tgtttgtgga atcttaccca cagttgctac tggtaaatgg
    21181 aacaatagca taactgctgt tgccaaaatg atagccagca gcgaactagg cattcgtttc
    21241 gcccattttt ttggatagat aaacatccct aaaatgacta gccctcccat gatcaaagag
    21301 gctgtgttta tcggaaatcc tatcgtttga taactactta gtttttcaat aacgtttgta
    21361 ccttccgagt gaacaccaaa taaattatcg atctgaccta atgcaataat gatagcaata
    21421 cctgaagtaa accccgtaat tacgggtgct gggataaaag aagtcagtgt tcctaatcgg
    21481 aaaagtcctg ctgcaagtaa aaatagaccc gctaaaaaag tagcaagcaa tacaccttgc
    21541 attccctttg ttgccgcaat agacatcaag atagctgcca tcgcacctgt cggacctgat
    21601 atttgataaa aaccgcctga taaaccgcca ataacgatac cggcaatgat agccgtaatc
    21661 aaacctgcgg ccgcatcagc tccgcttgat accccaaaag ccaaggctaa aggcaatgct
    21721 acagcagcta ctgtgatccc agccaaaaga tctttctgaa atcttgaaag atcataacct
    21781 gaaaattctt tttttagtga ggaaacataa tttttgaaca cctatctaac tcctttttca
    21841 tgaaattttc taaaatacca ttcattagaa tatcatattt catgagataa agtaatattt
    21901 ttcacaaagt ttcctgttag ataaattctt attttttcta tatttataag aaaatagaaa
    21961 gcaatgccaa cagagctagc ccaccttgtt tgagtaaaat ccaacgatcg ctagtcaaac
    22021 tgccatataa tgcaacaaaa aaaatagata ttaatagcat acttgtgatt tctttagatg
    22081 cggaagaaag ataagctcca taaagtagtc ccactcctaa tagtccattg tacactcctt
    22141 ggttcttaaa caatgtttga acagattttc tgcttaactc ttctttactc attttaaaaa
    22201 ccctacttgt cgtgtcagaa tcagttctca ccgtttcgat atacatgata taaaaaaatt
    22261 caattgcaac tagagtcact aaaattctag atagtatcgt catatcttct tcctcttttc
    22321 tgatgagttt tatttttttg ctccttataa ttgtactcga taaggatgtt tttttatttt
    22381 atttgcct
//
LOCUS       DAXWLH010000036        21761 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXWLH010000036 DAXWLH010000000
VERSION     DAXWLH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.036"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(177..290)
                     /locus_tag="ACGE36_000996"
                     /pseudo
     CDS             complement(177..290)
                     /locus_tag="ACGE36_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(673..1905)
                     /locus_tag="ACGE36_000997"
     CDS             complement(673..1905)
                     /locus_tag="ACGE36_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFF8383412.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2159..2728
                     /locus_tag="ACGE36_000998"
     CDS             2159..2728
                     /locus_tag="ACGE36_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8383413.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2906..3346)
                     /locus_tag="ACGE36_000999"
     CDS             complement(2906..3346)
                     /locus_tag="ACGE36_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFF8383414.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3504..4268
                     /gene="map"
                     /locus_tag="ACGE36_001000"
     CDS             3504..4268
                     /gene="map"
                     /locus_tag="ACGE36_001000"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFF8383415.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4300..5223
                     /locus_tag="ACGE36_001001"
     CDS             4300..5223
                     /locus_tag="ACGE36_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFF8383416.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIVPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5299..6438
                     /locus_tag="ACGE36_001002"
     CDS             5299..6438
                     /locus_tag="ACGE36_001002"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFF8383417.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6431..7231
                     /locus_tag="ACGE36_001003"
     CDS             6431..7231
                     /locus_tag="ACGE36_001003"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8383418.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7231..8058
                     /locus_tag="ACGE36_001004"
     CDS             7231..8058
                     /locus_tag="ACGE36_001004"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8383419.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8063..8770
                     /locus_tag="ACGE36_001005"
     CDS             8063..8770
                     /locus_tag="ACGE36_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8383420.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8870..9736
                     /gene="rfbA"
                     /locus_tag="ACGE36_001006"
     CDS             8870..9736
                     /gene="rfbA"
                     /locus_tag="ACGE36_001006"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFF8383421.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9750..10322
                     /gene="rfbC"
                     /locus_tag="ACGE36_001007"
     CDS             9750..10322
                     /gene="rfbC"
                     /locus_tag="ACGE36_001007"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFF8383422.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10344..11372
                     /gene="rfbB"
                     /locus_tag="ACGE36_001008"
     CDS             10344..11372
                     /gene="rfbB"
                     /locus_tag="ACGE36_001008"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFF8383423.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11470..12321
                     /gene="rfbD"
                     /locus_tag="ACGE36_001009"
     CDS             11470..12321
                     /gene="rfbD"
                     /locus_tag="ACGE36_001009"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFF8383424.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12356..14389
                     /locus_tag="ACGE36_001010"
     CDS             12356..14389
                     /locus_tag="ACGE36_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383425.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKQPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14433..15713
                     /locus_tag="ACGE36_001011"
     CDS             14433..15713
                     /locus_tag="ACGE36_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFF8383426.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15923..16729
                     /locus_tag="ACGE36_001012"
     CDS             15923..16729
                     /locus_tag="ACGE36_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383427.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16741..17961
                     /locus_tag="ACGE36_001013"
     CDS             16741..17961
                     /locus_tag="ACGE36_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383428.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17951..19537
                     /locus_tag="ACGE36_001014"
     CDS             17951..19537
                     /locus_tag="ACGE36_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8383429.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19576..21714
                     /locus_tag="ACGE36_001015"
     CDS             19576..21714
                     /locus_tag="ACGE36_001015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8383430.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6856 a         3691 c         4527 g         6687 t
ORIGIN      
        1 agtacttgtg ccaaattatg tgccaaaaaa attcgaattc aacaaaatgt aaacaaatag
       61 aaaaccttct atattaagat ttcctgcaat aacaaacaca acagaagacg tgtaatagtt
      121 agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa
      181 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
      241 actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga
      301 agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg
      361 attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc
      421 caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca
      481 atgggaatga actggagatg actttttatt atttttagta atagctgatg ggttaatact
      541 tacaactttc cttttcaaag taccatataa aaaaacggtg gggttgttta gcgaaataaa
      601 ggatctaagc caatagctta gatcctttat tctatgcttt atattatttt ttgcatgtgt
      661 gcgaaagtat actcaagccc aatcgccatt tcggaaaatc ggtactgtag agccatcttc
      721 acggattccg tcgatatcca tcttgtcaga tcctaccatg aaatctacgt gggtttgact
      781 tctattcaat ccagcttctg ctaattcttc atcggacatt tctgtaccgc ctttcacact
      841 gaaggcataa gcagaaccta gtgctaagtg attcgatgcg ttttcatcaa ataatgtatt
      901 gaaaaagatg atgccagatt gagaaatagg tgaagggtca ggtacaagtg cgacttctcc
      961 taaacgacgc gcaccttcat ctgtatcaag cagttttgct aagacatctt ctccttgttc
     1021 ggcagaaaaa tcaacaactt ttccatcttt aaaagtaaat ttcatacccg agatgatggt
     1081 gccagcataa ctcagtggct ttgtacttga aatgtaacca tctactcgat gactgtcagg
     1141 tgctgtgaac acttcttccg tcggcatatt tgccatgaat ttttctccac gagcattata
     1201 gcttccagcg ccttcccaaa gatggttttt aggcaatccg ataatgatat ctgtaccggg
     1261 agcagtatag tgaagggcag aaaattgttc tcgattcaat tcttctgctt tttttgctaa
     1321 tttttcatca tgcttcttcc atgctaagac agggtcttct tcatagacac gagtcgtttt
     1381 gaaaatctga tcccataatg cctcaacttg tttttcttct ggcagttctg ggaagacttt
     1441 tgctgcccat tgtttaccag ccgcagccac gacagtccaa ctgactttat ttgcttgagt
     1501 tgccttgcgt aaattcatta gtgctttgcc attcgctgat tgataagacg ctactcgatg
     1561 gctatctaca ccagcaaatg catcgggatc ggcagaaaca acgctgatcc tgcttgctcc
     1621 tttttcaagc cattcatccg cttgatcgat tttagattgt ggtacattct caatacgatc
     1681 cgttgcagca tgtaaaagaa attctctttg gatctggtcg tcagtccatt gaacgataac
     1741 ctcagcggct cctagtttat aagcttcttg tgtgatcaat cgagcaaggg gtgcctgttc
     1801 cacgctgatc tgtaaaacaa ctgtgtgtcc tttttctgtg gctacgccag tttctgcaat
     1861 taagcgtgca tattttttta gaagttcatt aaaatcagat aacataataa aaattcctcc
     1921 tcaccatttt ttctggtaac tattagcatg ataccattta attatgattc aaacaatgag
     1981 gctttaaata aaaaaaggag gttatttttt taaggttaag tttcatgata tatgttacta
     2041 cttgctattt cagcttgtga agaaaatcaa aaactaattt tgcatatcaa aaaaacatgt
     2101 tacaatgaaa ttaatcaatg gtggaagcgt tataaaaaaa ttaaggagat taaaaaatat
     2161 ggcaagaaaa aagacgatta ctagagaaca gattttggca gctgcttatg aagtagctgc
     2221 caccgatgga tttacacgat ttactgcgag aaacatcgcg aacaaaatga attgttccac
     2281 gcaaccgatt tatcttgagt ttaaaaatat ggatgacttg agacaagctc tttttgagca
     2341 gatctatgat catttacaat atgaagtgtt cactgttgag catacaggga atacgattat
     2401 cgatcttgct tttaattata tccattttgc aagaagagag aagaagttgt atcgttcact
     2461 gtacttagaa gaatatggtg gaggaaaaga gatgcagaat ttctcttata cttactttgt
     2521 gaatacagtg aaacgcgatc cagcttatgt gaatctttca gaagagcaga tcgaatcttt
     2581 gtataatggg acatggatca tagctactgg gatcgcagct ttgatgtctt caagcatcat
     2641 ccatccaact gatgaacaga ttgaaaagat gatccaagac agtatagatg ctatcttgaa
     2701 attcaaagaa ccaatcgaaa tcgattgaat atactcaaaa gttccacaaa tcagtaggta
     2761 acaagagatg tgtcaaaata aggaaaagtt cgtttttctt acagcgtatg catttttaac
     2821 tcccgaagaa ctcttccatg aatgcaatga ataaaagagc catacagatt gaataaatct
     2881 ggatggctct tttattgcaa gtatattaaa tttttgcgac tagttcttta gcgaaagctt
     2941 ctaaattttc gatatcttct tcttcagcat tcagatccac tttaacattt tcggctcctt
     3001 ttgatgcgcc tgtttttgca aaagcagcat cgaaatcatc caccgatttg cagaattcat
     3061 cgtaaaaagt atcaccagat ccgcagacac cgtaaatttt tcccaataga tccaattctt
     3121 gcaagtcttc ataaaagtct acgatctcat caggcagatc gccatcatcg tatgtataag
     3181 tagctactac gcaaatatct gcctcctcga attcatctgc atctacttgg gtacattcgt
     3241 tgatttcgac ttcgatatcc agattttcaa atgcttctgc aacaatatcg gcgatttctt
     3301 cagtgtttcc tgtcatgcta gcatatacga tttttgctaa agccatgatg tgcctcctcg
     3361 tttataatta tttaacaatt atgaattata ccaaaacatc attgaaaaga aaataaaacc
     3421 taaagacacc tggggaatgt catttggcta aatgtatgcc gctatgttaa aatgagaggg
     3481 atttgtaaaa aggagacgaa aatatgatta ctttaaaatc agcacgtgaa attgaaatga
     3541 tggcagaatc tggagcttta ttagcagatg tacataaaaa tctgcgtgat tttataaaac
     3601 caggaatcac aagttgggat attgaggtat ttgtcagaaa ctatatcgag agtcatggcg
     3661 gaattgctgc gcaaattggc tttgaagggt atgaatatgc gacttgtatc agtataaatg
     3721 atgaaatttg tcatggattc cctcgtaaaa agcctttgaa aaacggtgat ttggtaaaag
     3781 tcgatatgtg tatcgatttg aaaggcggca tttctgattc ttgttgggcc tatgtagttg
     3841 gagaatctac acctgagatc gaccgattga tggaagtaac gaagaaagct ttgtacatcg
     3901 gtattgagca agcacaagta gggaatcgga ttggtgacat cggtcatgct atccaaactt
     3961 atgtagaagg cgaaaatcta gcgatcgttc gtgattttat tggtcatggc gttggtccta
     4021 cgattcatga agaaccagca gttcctcatt acggagaagc tggaaaaggg ttgcggttga
     4081 aagaaggcat ggttatcacg atcgaaccta tggttaatac cggaacttgg aaaatgaaaa
     4141 tggatcctaa cggctggact gcgtatacaa gagacggcgg actttcttgc caatatgaac
     4201 atacattggc tattactaaa gatggtccaa gaattttgac atcacaagga gaagaaggca
     4261 cttattgatc tatccagtca ataggtacgg gagaggtaca tgaaagtttg gaacaaaata
     4321 aaacaaaatg aaaatctgat gcgctttatt gagacgactc aaaagcgcat cactgattca
     4381 gaaatgagta cgacctctgt cgtcgttgct tattatttac tcttgtcttt atttccattg
     4441 attattgctt ttggtaatat tttaccgttt ctccacatcg atcaagagac tgtactgacc
     4501 tatttacgag aagtgattcc tgagaccgtt taccagttta tcgttcctgc aatcaaagac
     4561 cttttgacac aaagttcagg aggcttgtta tccgtttctg cattagcggc tttatggtct
     4621 gcaagtcaaa gtatcaatgc tttgcaaatt gcgatgaata aagcgtatgg cgtagaaaat
     4681 agaaaaaatt ttttgatcgt ccgtttcttc tcactaattg tcattgtctt gttcatgatt
     4741 gctattagtg gggtaactat cgttcttggg ttgggacagc tgattttgga tgctattcag
     4801 ccaatcgtac aaatacccgt gaactttatc gatcagttcc aaactttgaa gtggccaatc
     4861 acattggtag ctttatttgt catcatgttc ttgatttatt taattgtccc taatgctcag
     4921 ttgaagttga agacgatcat tccgggcacg atcttcgcta ctgtcggctg gatgctgtta
     4981 tcacaagtgt ttagtatcta tgctaggtat tttgcaacga ggatcagcgg ttatcagatc
     5041 ataggaagtt ttattgtact catgctttgg cttaattttg ctgctacgat catcattttg
     5101 ggcggaatca tcaatgcagt tgttcaagaa tacgtgacgg gtcatcaaat caaaaaacgt
     5161 cgagatccaa gccgaaaatg gttcaataaa ttgaaaacta aattttctca taaaaaaaag
     5221 tagaaacaag agttcttatt gtaaatttat atcagtaatt tataataatt atgactctta
     5281 attaaagtag gtgcccatat gcttgtttct tttagtatgg ttataagatt ttttctgaca
     5341 attttattat cactgatatt aacacctgta ttcaaaatca tttcagtaca aacaggtatg
     5401 gtcgacaaac caaatgaaag aagaataaat aaagttccaa tgccttcagc gggcggactg
     5461 cctatcttta tttcatttgt tatatcgact ttattcttat tccgtaaaat tatccctcaa
     5521 tcgtatattg taccgatctt gattgcttct ttagtaatca tagtcacagg agtgctcgat
     5581 gataaatatg agctgacacc taaacaaaaa agtataggga tcttgattgc ttctcttatt
     5641 atttattttg tagcagatat acgtattgat tccttcacgc ttccttttat tggatatatc
     5701 caactaggct ggcttagttt tcccgtaacg attttttgga tcttcggtat cacgaatgcg
     5761 gtcaatctga ttgacggttt ggatgggctg gcggcaggga tttcattgat cggtttaatt
     5821 acgataggaa tcattggata ttttttcctc catgcttcta cagtgtatat tccagttgtt
     5881 attttctgtc ttgtagcaag tattatcggt ttttttcctt acaattttta tccggcaaag
     5941 atttatttag gcgatacagg agctctattc ctagggttta tgatggccgt cctttcacta
     6001 caagggctga agaacgtgac ttttgtttca tcgatttctt tgctgatcgt catgggagtg
     6061 ccggtaacag acacgttttt tgctatcatt aggagaaaag caaaccgtgt ttctttttct
     6121 acagcagata aaaaacatct tcatcaccga ctaatcagtt taggctttac tcataaaggt
     6181 gctgtcctga cgatttatgc gatggctttg atgttttctt tcacagctat ggtcatgaat
     6241 tatactggaa gaatcggaac gatcatattg attattgcca tgttgtttgc tgcaatttta
     6301 ttatttgaat tgattggtct gatcaatgag aaacatcagt ttattttaaa aacattgcga
     6361 tttttaggga ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac
     6421 cgtcatatag atgaataaca aattaaacca gccgatcctt attagtattg tgacggcaaa
     6481 cagtgataaa atttttcgga cactagataa ttttatttca actgtaaaag atgaaacagc
     6541 agtaaaaatc cgaatttttg acaacaattc acttcccgag tacaaaaaaa gactaacaga
     6601 gtataggaaa tatccctaca tcgatatcta ttttcatgat gaaaattccg gatttggtta
     6661 tggacataat cacaatctga tagctagcga ttctcgctat ggcattattt gtaacccaga
     6721 tattttagtg gatgaagaga cagttacagc attggtcttg ttattgaaag aacatccgga
     6781 atgtgcgatg ttagcaccta agattcttaa tgaagatgga acaactcagc acctcatccg
     6841 tgagcagctg gctgtttttg attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa
     6901 aaagtttgac aaacgactgg ctgtgtttga atgccgagga ttgcctactg atgctaatag
     6961 ttatgtacgg atgatctcag gtagttttat ggtagctgat attgataaat ttaaagagat
     7021 aaaggggttt gatgagcgtt tcttcatgta ctttgaagac aacgacttgt gtttgagttt
     7081 tgaaaaagca ggttacaagt tattgtacac acccttttat gcagttatcc atttttacgg
     7141 gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt
     7201 tttcaataag tgggggtggc gttttttctg atgaaaccag aaatattagt agtcgttgtg
     7261 ctttatcagc aactattcag tcaatctcca gcttatggac cattaagcaa agcacttagc
     7321 gaaaagcaaa ttcagcttat tatttacgat aatagtcctc aaaggcagca aaatgagctg
     7381 tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa atcctggttt agcggtggct
     7441 tataattttg cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac tctggatcag
     7501 gatacgaggc tagtcgacga ttattttgaa ctattgcgta aagtaacctt tacagatgaa
     7561 tgtgttgctg cagttccgat gattttttct gggagccgac aagtttctcc cctatattca
     7621 gatcgttata tcaatcgtac agctcaagca gtagaagttg aaggcccaac agctgaacga
     7681 atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg
     7741 tttaatcctg ctttttcttt agattttttg gatcattggc tgttttggaa agtggatcag
     7801 ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac atgatctttc ggttttagac
     7861 tatgaaaaag tttcaagtaa tcgataccgt tctattttac aagcagaaaa tcgattttat
     7921 catgaatatg atcaaaggca tttatccagt caccgacgac aattattgtt aagaacagct
     7981 aaacagttct tgaccgtcaa aaaccggcat atctggcgga tgactttgcg ctcatatgtt
     8041 aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta atggaggcag
     8101 atatttaaga gagcagttag atagtatttt atctcagctt tcagcagaag atgaagtagt
     8161 catttctgat gatgggtcag tagatgatac atgggatata ttaactgaat acgcagaaaa
     8221 agataagcga atccagcttt tttcggggcc tcgtcaagga ttgatcgcta attttggtta
     8281 tgcgatccag cagacgaaag gagatctaat ctttttggca gaccaagatg atgtttggtt
     8341 ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag aatccggatc agctagtagt
     8401 agtttctgat ttaatcattg tggacagcga gctgcaagag atccatgatt cgtatttttc
     8461 gtttcgaaat tccaaagaag gatggcttaa taatgtgata agaagtcact atattggtgc
     8521 tggtatggct ttccggtctt ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc
     8581 gatgcatgat atgtggattg gtttgctggg aggaaagaag actgggttcc taagagaacc
     8641 gttaactctg taccgaagac atgatttaaa cgcgagtgag attgaaacac attctaaatt
     8701 acggcaaaaa atttcttggc gtgttcatct gattacggcg ttgatcaaac gaaaatggtt
     8761 taaacgttga taaaagatgt ttaaatcctt aatatttcca tgacaaatat aacaattgat
     8821 gagataataa caaagtgaaa tttgaaatca aaaaggagag cagatatcca tgaagggaat
     8881 cattcttgct gggggcagcg gaacacgtct gtacccttta acaaaggcaa catcgaaaca
     8941 attgatgccg atttatgaca aaccaatgat ttattatcca atgtctactc tgatgttggc
     9001 gggaatcaaa gaaattctga ttatttcaac gccacaagat acaccacgtt tcaaggaatt
     9061 atttggaaac ggaaatgatt taggactgca gatcgaatat gcagtacaag aaagtccaga
     9121 tggattggca caagcgttta tcattggaga agaatttatt ggagatgata gtgtctgctt
     9181 ggtattaggg gacaatattt actatggtgg cggtctgtct aaaatgttgc aacgagcagc
     9241 ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc aatgatccag aacgctttgg
     9301 tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc
     9361 aaaatcaaat tacgctgtaa ctggccttta tttttatgac aatgaggtaa tttctatcgc
     9421 taaaggtatc aaaccttcag aacgtggaga actagagatt acggatgtca ataaagcata
     9481 tttggataaa ggaaaactat ccgtagaact tatgggacgc ggatttgctt ggcttgatac
     9541 aggaacacat gaatcattgc ttgaagcatc tacctttatc gaaacaattg aaaaacgtca
     9601 gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt atgggctaca tcgataagga
     9661 acaattgctg gcccttgcac agccattgaa aaaaaatcaa tacggtcaat atttattgaa
     9721 tttggccgct gaataaggag tccttacaga tgaaagtaac agatacaaaa ttaacagatg
     9781 tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg tttcttcaca gaaagttatt
     9841 caaaagcaaa atttgcagaa catggattga aatatgactt tgttcaagac aaccattcac
     9901 tatcagctga agctggtgtc cttcgggggc ttcatttcca aaaaggagaa gctgcacaga
     9961 caaaattgat tcgggtagtc actggagcag ttttagatgt agtagtggat atccgcaaag
    10021 gtagcccgac ttatggtcaa tgggacggat ttatcttatc tgaacataat catcgacagt
    10081 tgcttgtacc taaaggatat gcccatggat tcgttacttt gacaccaaat gtgaacttcc
    10141 tttataagtg tgataattac tataatgcag aagcagatgg gggcatcgca tttgatgatc
    10201 cagatttagc gattgattgg ccaattgatc catcaagagc gattatgtcg gaaaaagatc
    10261 accgtcatcc gacattaaaa gaatttgaag cagaaaatcc atttgtttac ggagaaatct
    10321 aagaattaag gagaaatttt cttatgaaaa acatcattgt taccggaggt gccggattca
    10381 tcggctctaa tttcgttcat tacgtggtaa acaatcatcc agaagtgcat gtcactgttt
    10441 tggataaact gacttatgca ggaaacaaag agaacttggc cggacttcct agcgatcgcg
    10501 tagaattggt cgtaggagat gttgcagatg cagaattagt ggaccgtcta gtaaaagaag
    10561 cggacgcagt tgttcattac gcggcagaat cccataacga taattcatta aaagatcctt
    10621 tcccatttgt acaaacgaat atcattggta cgtatacact tttagaagct tgccgcaagt
    10681 atgatgtacg ctatcatcac gtatctactg acgaggttta cggcgatctt ccattacgtg
    10741 aagacctgcc aggacatggg gaaggcgaag gagaaaaatt cactgcggaa acaccatata
    10801 atccttcaag tccatattct tctacaaaag ctggttcaga tctgttagta aaagcatggg
    10861 tgcgttcttt tggcttacgt gcaaccattt caaactgttc aaacaactat ggtccttatc
    10921 agcatattga aaaatttatt cctcgccaag taacgagtat cttaagcggt attcgtccaa
    10981 aattatatgg cgaaggaaag aatgttcgtg actggatcca tacgaatgat cattcttcag
    11041 cagtgtggct gattttgacg aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg
    11101 gtgaagaaga taataagacc gtgatggaat tgatcttaga aatgatgggc caaccaaaag
    11161 acgcatacga ccatgtaaac gaccgcgcag ggcacgattt gcgttatgca atcgattcga
    11221 caaaattgcg agaagagcta ggttggcagc ctgagtttac gaacttccgt gatgggttgg
    11281 ctgacacgat caaatggtac gaagaacatg aagactggtg gaaaaaagaa aaagaggccg
    11341 ttgaagcagc atatgccaaa aatggacaat aatacagagg aaatttcact gtattaatgg
    11401 aaaaacacat gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg
    11461 gataaccata tgtttttatt aactggagga aatggacaat taggtacaga attgcgccat
    11521 ttattagatg aaaaaaatct gccatatgtt tcaacagatg caaaagactt ggatatcaca
    11581 gatgctgaga aaacaatggc ttatattaca gaattaaaac ccgatgttat tttccattgc
    11641 gcagcttaca cagcagtaga taaagcagaa gaagaagcaa aagaattaga tgagaaaatc
    11701 aatgtcgatg gcacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc acaattcgtc
    11761 tatatcagta cagactatgt tttcgatgga aaaaacaaaa atgtagaata cgaagtagac
    11821 gaccaaacga atccactaaa tgaatatggt cgtacgaaat tattaggcga aaaagcagta
    11881 gaagagattt tagaagacta ttatatcata cgaacttctt gggttttcgg gatctatgga
    11941 cacaatttcg tcttcacgat gcaacgccta gcggaaaccc gcgacaaact gactgtagta
    12001 aatgaccagt ttgggcgacc aacttggaca cgtactttgg cagaatttat ggtatatgtc
    12061 atcgaagaaa aagcaccatt tggtatttat catttgtcga acgaaaatag ctgttcttgg
    12121 tatgagttcg caaaagaaat attgaaagat acagatgtag aagttgcacc tgttacgtca
    12181 gaagaatttc cgcaaaaagc aacacgtccg cagtattcag tcatgagcct gaaaaagaca
    12241 gaagcgttag gttttgtcat accaacttgg caagaagcac ttgcgcaaat gctggaagct
    12301 gccaaaaaaa tgaataagta atttcctttt tatcaaatag aatttggagc atgatatgga
    12361 aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaatcagg cagactcgaa
    12421 tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag tcatcgtggt
    12481 catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat atgtgtcgca
    12541 aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag tgcggtcaga
    12601 cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact tttcgttggt
    12661 gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt ctcctgtaaa
    12721 ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg gaagagatcg
    12781 aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt
    12841 actgatgata ttaacgaaga ggaaaaaagc tctatcgcta atcggtgcat ttgcgctgac
    12901 attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga ttttctttac
    12961 tttgggactg atgattgctt tttatcatta tttctacaat catgataaaa aatggctacg
    13021 aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag tgatttaccc
    13081 agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc tgcttattta
    13141 ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag gggcagtttt
    13201 atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat tgttagcttc
    13261 tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga cgattggcga
    13321 atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact attcgaataa
    13381 ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa gtccatttgt
    13441 gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga tgattttttg
    13501 tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag aaatcactct
    13561 gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg cttgtttatt
    13621 atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt ggtataaatt
    13681 cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt cgaaaatgga
    13741 taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag tgattttagc
    13801 ttttgcatta tttggctgga gacgcttgtt ttacctcgct tttgtcgcct tagttatcgt
    13861 atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg aaaaaacact
    13921 ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt tgtctgaaga
    13981 tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag tacgctttta
    14041 tccagatata gctgcatgga agaaaatcga tccgaaagaa gaatacgaga agtattataa
    14101 tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca ttttagatcg
    14161 tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag ggatcgacta
    14221 tgtgatcagt aaacaaccat taactgatta caaccaatta tatgaagcgc agttcacgca
    14281 gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc cggattatcc
    14341 agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat aggctagctg
    14401 agaataatcc tgaaatatta gtagaaagga gagtgaagat aattcaagca atcgtacaaa
    14461 aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt gctgacgcta gtcttttttg
    14521 tttggacagc aggggtaggt tttccagcaa ctttgtttat atataacaat agttcattac
    14581 ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca aaaggcgtcg
    14641 attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac tttcgggaat
    14701 taagaaatga gtctttattt ggagacttac tgtttccttt gattatttta ttttgtttag
    14761 taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt gtctttacgg
    14821 tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca gaaggacaaa
    14881 gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat acaatcggaa
    14941 gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt gaacgatggt
    15001 atgttcgaat catcggtggg atagcagtcg ttgcagcctt tttaggtatt tgggtctgcc
    15061 aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt ttacctaaat
    15121 cgttgatgcg tgtcattcgt gcgccattta ttgcctacac ggttacgatt cttttagctt
    15181 tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag
    15241 atatttggca taaattctat cagacgctag gagaaaaatc tgaacaagtt ttactaggaa
    15301 tgaaaacatt tatcttccag cgaggaaacc aatttttagg aaaccacaat agttataatt
    15361 ctattttgaa tatctatgga ttgataggat ttgggattgc tgctttgctg cttatccttt
    15421 ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact tttatctggg
    15481 ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca tacccatggg
    15541 ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa ccagaagtta
    15601 cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat gatacatctt
    15661 atgaggaaca accagatagc gctcattctt ccaggatcca acgctatcat tgataggcac
    15721 aaaatacgag agaggctgtg acaaccgtcg cagccttttt tggtttattg caattgtcta
    15781 gcatcttctg taaaatagaa acgttagaga aaatcttgta tcatagaaaa ttaagaaaga
    15841 aataagaacg cagctcggta ccaaaaatat ggtaaaatgg cacttgactg taattcactg
    15901 aaaatcgctt ggaggaacac aaatgtttaa agatttgtat ttatttatga aggacattta
    15961 tcagaacaga aaattactgc tacagttttc gatcaatgac ttcaaatcaa gatatgcagg
    16021 atcgttttta ggaatcttat gggcgtttat caatccgcta tttactgtat tgatttattg
    16081 gctggtattt ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc catttatcgt
    16141 atatttagtt accgggatga ttccctggtt cttcttctca gatgcatttg catcaactac
    16201 ggttgttttt cgggaatata cttatttagt aaagaaagtc gtattcaata ttcgtatctt
    16261 gcccacgacg aaaatcctat ccaatctgta cacccattta ttcttcattc tgattggcat
    16321 agctgtcgcg cttataaatg gtatatatcc ttcagcaatg tctttacagc tgatttatta
    16381 tttattttgc atgtgtgctt tcttgacagg attgacttgg ctgacggcat cgatccaacc
    16441 atttttacct gatatcatgc aattaattac gatcgtgctg cagttgatca tgtggacatt
    16501 accgatatta tggagcccta atcagtttga ttcaggtctt gtaagcatat tgaaattaaa
    16561 tccgctgtac tatgttgttc aaggttatcg tgagtcattt ttaagtgaag cttggttctg
    16621 ggagcatgga acatatactt tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg
    16681 ctctgcagtg ttccgtagat tgaaaccaca tttttcagat gtgctgtaag gagaatagct
    16741 atgacagaat atgcaattag actaaaagat atcacaaaat cttataatat gtacgcaaaa
    16801 ccttcggatc gatttcgtga agccctcaat ccttttaaga aatcctatca tgatgtattt
    16861 tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga
    16921 gaaaacggat cgggaaaatc tacaatgttg aaaatcatta cgggagtact gacaccaaca
    16981 tccggcacga tggaaatcga aggaaaaatc tcagctttgc tagaactagg atcaggattc
    17041 aatcctgagt attctggcta tgaaaatatc tatttaaatg gaatggttct aggattttca
    17101 agagaagaag tcgatgaaat ggtcgatgat atcattgaat ttgctgacat cggcgaccat
    17161 atttatcaac ctgtaaaaac ctattcaagt gggatgttcg ttcgtctagc ttttgctgta
    17221 gcgatcaatg tgaatcctga tattttgatt gtggatgaag cattggcagt aggagatctt
    17281 gaatttcaac taaaatgtat ggaaaaattt acagaaatca agaactcggg aaagacgatt
    17341 ttattcgttt ctcatgatat tagttcgatt cgccgttttt gtgatcgttc ctattggttg
    17401 aaaaatggaa aagttgtcga atatggtgat acgatggaag taacgacgaa ttacgaaaac
    17461 tttttgaaga aaaaatcagt taaaacagta gatcgaaata aaaaccaagt agaacgcttc
    17521 gcagctccag atatcgtaga cgtgacgaaa gccgaattgt tggataaaga taaaaatcca
    17581 gtggatatgc tggaacaagg agaaaaacta tacgttaaag taacctatga agtaaaagac
    17641 gatacaatca aaaatccagt tttaggaatt gctctgcgta cagtcgacaa tttttatgtg
    17701 tgcggactca atactctact agatggtatc aagatccctt ggaaaaaagg tgagaatatc
    17761 ttttatctgg agtatgaaaa gttaggtttg ttagctggtg aatattattt tgacgttgcg
    17821 gtctttgaag aaaatgcaac ggttcctttg gtatacaaga caaaatacat gaacttgttt
    17881 gtcagcggtt cttatatcgg agaaggcatc gtggtcttag accataaatg ggaagaaggg
    17941 actcatagca atgaaatatg attttgaaat ggacctagat gaacaaagca gtgtgggaaa
    18001 aattgccgca cagatcaaac caggaagtaa agttttagag tttggtcctg gaaacggccg
    18061 attaacgaaa catttgatcg gagcaaaaca atgtgaagtg agcattgtcg agctagataa
    18121 ggaattgttc gattttgtca gcgagtttgc ccaagatgga ttttatggag atatcgaaag
    18181 ttttgaatgg gcaaactatt atgctggaca aacatttgat tacgttttgt ttgccgatgt
    18241 tttagaacat ttggtagatc ctggaaaaac gttgaagaaa gttagagaat ttttgaatga
    18301 agaaggagaa attctaatta cattccctaa ccttgcgcat aattctgtca tgattgattt
    18361 gttcaataat caattgccat gggcttctta tggtttgcta gatgaaaccc ataattcttt
    18421 ttatactcat gatggcttcc aaaaagtttt tgaaaaagca ggattataca tcaatattga
    18481 agattatttg tacttagctg taggtgatac agaattaaaa tcaacttatg aagaattgcc
    18541 ggaagctgta cgttatgatt tcaaaatgcg accatttggc gaagtctacc aatacttctt
    18601 ctcattgatg aaacatccag tagcacaaag ttccattgcg cagccgcaaa attctaatta
    18661 tgtgaaagta ttagaagtaa tccagcaaac gaaacaagat gaaataattc aacaaatacc
    18721 attcaataat ttcacagggg aaaatgagac actaagtttc catgtatcag aattagcaga
    18781 acgaatggtg tttcgcttcg cgcagcaacc tagctttatc gaattcagcg cagaagctgc
    18841 tggagaaaaa ctcacattta tcgattcaaa tgctgtagtc aaaacagtaa atgattgtta
    18901 tttgtttgat ggtaaagatt taccagagtt ctttttaacg gatattgcag ggaaagaagt
    18961 gactatccat tgtcactatc gttttatcgg agaattgaca ccaacaatga aagaattgct
    19021 ggaaacaatc cgtccaatgg ctgaagttac aaagcagctt tctgagaaaa atgatgaatt
    19081 agaaagagaa aatcattatc ttattgagaa taatacaaga ttagatcaga cgttgaaaac
    19141 aaccataaat cgctattgca cgttgttgga aagtgatgaa tggacaatca aacatagatt
    19201 agggcgacgt aagaaagaaa catcgaaaaa aatccaagaa aaagagcttt ctctatgtat
    19261 tgacgaaaaa atctgggatg cagaaacaaa gatattgaaa atcatcgggt ggggaatcgc
    19321 taattcggat cgccaaccat tgtcctataa gttatcagcc gaccaatcac cattttttga
    19381 agcaattcct gttttacgag aagaagtcaa tcaagcaaaa cagctagctg aaggagcaaa
    19441 agcaggattt gaactgcgga tcctttgcga gaaggaacgt tcattcttag ttgaagcagt
    19501 ggctcaaaat ggtcaatcat ggatcattga aatgtaaata atgattaact tataaagata
    19561 aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca aatcactcgt
    19621 gaacgggcca ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa
    19681 atccctacga taaaggtaga aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga
    19741 ttggatatca atcatttata taatcttgat gttaagaccc aaagcggttt taaggtacgt
    19801 ttatcaggga aaatgagagg aaaagcaatc ttagacttcc aaacagcaag acatcaaaat
    19861 ggcatagctg tgaaattaaa cggtaaatat ccttatgatg acggcattga atcttcatgg
    19921 gaaagaaaaa aaagattact gaaaaaaggg ataaattatg caaggactca tggcgtaaaa
    19981 aaagcaatcc gccgggtcaa actggaattg aaaccaggat cgatcgacta tgcagagtgg
    20041 atcagtcgtc atgagaaacc tgatctgaaa gagcaaagga aagaagtaca aggatttgcc
    20101 taccgcccat tgatttccat acttatgcct gtctataacg ttgaaatcaa atggctcgaa
    20161 aaatgcattg attcggtact tgatcaaacg tatgatcact gggaactatg tatcagtgat
    20221 gatgcttcta ctgatccagc tatccgaaag tgtctagaat cctatcaagc aaaagatgat
    20281 agaattaaag tcgttttccg acaagaaaat ggacacatca gtctggcaac taattctgca
    20341 ttgaaaatgg cagaaggaga gtttatagcg ctcttagata atgatgacga attacctcca
    20401 tttgccttat atgaagtggc aaaagtgcta aacgtacatc cagaactgga tttgatctac
    20461 agtgacgaag ataaaatcga tgcagatggg aatcgctttg acccgcactt caaagcagac
    20521 tggtctcccg atacattgat gggaaataat tatatctctc acttaggcgt atatcggaca
    20581 agcattgtta aggaacttgg cggtttccgt aaaggatatg aaggctctca agattatgat
    20641 ttggttcttc gtgtgacaga acaaattcct gcagaacaca tttaccacat tgaccgtgtc
    20701 ttgtatcatt ggcggacgat cccgggatca actgcaagca atggagaagc taaaagctac
    20761 atctatgatt ctggagtgaa agcattgaca gatgctctat ctagaagaaa tatcaaggga
    20821 agcgtacatc ctggcagaat ttctggtttt tatgaaatca cttatgatgt tttgcaagaa
    20881 gacttggtca gtgtgatcat tccaactaag aatggctatg aagatctaaa aacttgtgtg
    20941 gattcgatca tcgaaaaaac gtcttatcca aattatgaaa tcattattgc agataatgga
    21001 agtacagatc ctaaaatgca agaattattt gcagaataca agcatcagct gaaagaccga
    21061 tttatcgtcg aattaattga cattccattc aattattcac gtatcaacaa tctggcagca
    21121 gaaaaagcaa acggcaaata ctttttattc ttgaataacg atacagaagt gattgaacct
    21181 gactggatga cagcgatggt ttcttatgca cagtttgatc gaatcggctg tgtaggagca
    21241 aaattatttt atccggatga tacaacgcag catgcaggtg tattattagg gatcggtggc
    21301 gttgcaggac atgcattgaa taactatgat cgaacacatt gtggttactt tggccgattg
    21361 gtcattgacg tcaattactt agcagtcact gcagcttgta tgatggtcaa ggcagctgac
    21421 ttcaatgctg tcaatggatt tgatgaaata ttagaagtag cattcaacga tgttgacttg
    21481 tgcttgaaag tttatgaact gggtcgttac aatgtctatg cacatcaagc agaattgtac
    21541 catttcgaat ctaaatcaag aggttatgaa gatacaccag aaaaacaaag acgttttgct
    21601 ggggaaatca aaaaaatgca agataagtgg ccttcttata ttgctcatga cccattttat
    21661 aacgataatc taactaaaga aggaatcggc gatttttcgt tgagacctga ttgattaaaa
    21721 gaactgtatt ttcattttta ttaatgaaaa tacagttctt t
//
LOCUS       DAXWLH010000037        21467 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXWLH010000037 DAXWLH010000000
VERSION     DAXWLH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.037"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(212..2593)
                     /locus_tag="ACGE36_001016"
     CDS             complement(212..2593)
                     /locus_tag="ACGE36_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8383431.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITERTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2583..3230)
                     /gene="recU"
                     /locus_tag="ACGE36_001017"
     CDS             complement(2583..3230)
                     /gene="recU"
                     /locus_tag="ACGE36_001017"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFF8383432.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3294..3833
                     /locus_tag="ACGE36_001018"
     CDS             3294..3833
                     /locus_tag="ACGE36_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFF8383433.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3923..4342
                     /gene="gpsB"
                     /locus_tag="ACGE36_001019"
     CDS             3923..4342
                     /gene="gpsB"
                     /locus_tag="ACGE36_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFF8383434.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4364..4731
                     /gene="rnpB"
                     /locus_tag="ACGE36_001020"
     ncRNA           4364..4731
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGE36_001020"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4940..6106
                     /locus_tag="ACGE36_001021"
     CDS             4940..6106
                     /locus_tag="ACGE36_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFF8383435.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6152..7648
                     /locus_tag="ACGE36_001022"
     CDS             6152..7648
                     /locus_tag="ACGE36_001022"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFF8383436.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7729..8412
                     /locus_tag="ACGE36_001023"
     CDS             7729..8412
                     /locus_tag="ACGE36_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8383437.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9057..10247
                     /locus_tag="ACGE36_001024"
     CDS             9057..10247
                     /locus_tag="ACGE36_001024"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFF8383438.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10349..11302
                     /locus_tag="ACGE36_001025"
     CDS             10349..11302
                     /locus_tag="ACGE36_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFF8383439.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11371..12720
                     /locus_tag="ACGE36_001026"
     CDS             11371..12720
                     /locus_tag="ACGE36_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8383440.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12903..13226)
                     /locus_tag="ACGE36_001027"
     CDS             complement(12903..13226)
                     /locus_tag="ACGE36_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383441.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13355..14518
                     /locus_tag="ACGE36_001028"
     CDS             13355..14518
                     /locus_tag="ACGE36_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8383442.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASRQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14588..15937
                     /locus_tag="ACGE36_001029"
     CDS             14588..15937
                     /locus_tag="ACGE36_001029"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8383443.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15982..16413)
                     /locus_tag="ACGE36_001030"
     CDS             complement(15982..16413)
                     /locus_tag="ACGE36_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383444.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16801..19443
                     /gene="alaS"
                     /locus_tag="ACGE36_001031"
     CDS             16801..19443
                     /gene="alaS"
                     /locus_tag="ACGE36_001031"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFF8383445.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19643..20239
                     /locus_tag="ACGE36_001032"
     CDS             19643..20239
                     /locus_tag="ACGE36_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8383446.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20361..>21467
                     /locus_tag="ACGE36_001033"
     CDS             20361..>21467
                     /locus_tag="ACGE36_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8383447.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDY"
BASE COUNT         6940 a         3786 c         4539 g         6202 t
ORIGIN      
        1 attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg gattttctaa
       61 tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata aaaaatcatg
      121 taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac acaaaaaaga
      181 aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct gaggtgctgc
      241 tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca ctggaagaag
      301 caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg
      361 ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac tcagcagttg
      421 attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt gagcttccag
      481 gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca ttcgatctag
      541 ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg cttggcatct
      601 cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga taaacatcag
      661 aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa cctgtccaca
      721 cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac accccagaag
      781 aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc ttaccggctt
      841 gatataaccc agcgatttgc gcattcgtac cagttctttc agtgatcgta tcttttaaaa
      901 tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct ggctcataag
      961 tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg taataagtcc
     1021 ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct ccgtattttg
     1081 tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc gtactgtatt
     1141 cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca ttgaataatt
     1201 tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg gtgcccatat
     1261 attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac ggtgcgtcag
     1321 taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa tctgtgacag
     1381 gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg cctaaagtta
     1441 cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta ttggtgtcga
     1501 taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga tccgtgttga
     1561 cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt
     1621 ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct ttgacataat
     1681 tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca tccgttaccg
     1741 gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga agcatcgtat
     1801 acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga tcataggcgg
     1861 aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt
     1921 tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt gacatataca
     1981 ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc aatcttacag
     2041 ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg gtggagaaga
     2101 aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa
     2161 agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt ttataaaaac
     2221 ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa
     2281 ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt gtatatagtt
     2341 tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct tttgcgtaat
     2401 accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt attcctaaaa
     2461 ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa cctttgccag
     2521 aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag cttctagttt
     2581 gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata gaagtcaacc
     2641 gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc tagttcttcg
     2701 atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc
     2761 aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa caagacaaag
     2821 cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc atggaagttt
     2881 ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa gtaaaagcct
     2941 cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc cttgatcaca
     3001 gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg ctttttatgg
     3061 ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc ttcttcaaag
     3121 cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat tttttcgacg
     3181 ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat ctttaatcac
     3241 actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct gagatggata
     3301 agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt tttaaagaaa
     3361 atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat cagttagcag
     3421 aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc
     3481 aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt ttgtatcctt
     3541 tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac
     3601 aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct agtcagctaa
     3661 agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc tatgatgaag
     3721 aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa gcagatcatc
     3781 cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa taatcgatat
     3841 tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg ttattacgta
     3901 aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag acattttaca
     3961 acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg aattcttaga
     4021 caatatcatc aaagattatg aaacttacag caaagaactc cttgctttac aagaagaaaa
     4081 cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag ctcaaacacg
     4141 cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata tcttaaaacg
     4201 cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag caagtgcagt
     4261 caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct tagatgataa
     4321 tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg ggtaatcgcg
     4381 gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt
     4441 tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg aaaacaagct
     4501 aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc ggctggggaa
     4561 acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat ggtaggggcg
     4621 ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata gatggttacc
     4681 gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga aaaccgcaaa
     4741 catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa aaacatttga
     4801 aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact tttgtatcac
     4861 cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc tccttttttt
     4921 ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg caactgcggc
     4981 aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg aatgccaagt
     5041 tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag ctaatttatg
     5101 gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga
     5161 tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac tagatgcagc
     5221 ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg attgccaagc
     5281 gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt
     5341 accactgacg gaaactggcg cacattttca gttggaagta gctttgctga aagaccatgt
     5401 gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc gcttagaaaa
     5461 aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga caaattggcg
     5521 aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta ttgaagcagc
     5581 cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg aatcttggga
     5641 ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa aagcagatta
     5701 tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg aaattgccaa
     5761 agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac aagcagtcaa
     5821 agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt acggagaacg
     5881 tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg tattccgtcc
     5941 attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg aattttacgg
     6001 acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg atctattcca
     6061 gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt actaattgat
     6121 tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt tacaagaagt
     6181 aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata cacaaacagg
     6241 tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt acagcattta
     6301 tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt ctgaacatcc
     6361 ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag agtatgattt
     6421 ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt cttcagcaca
     6481 cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac acgcattgaa
     6541 tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg aaaagacgaa
     6601 ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc ttgataaggt
     6661 atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag aaaaagggac
     6721 tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga agagatttgt
     6781 agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag atgacactgt
     6841 ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac gatggaacga
     6901 aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg gtatgtatga
     6961 acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat caatgggtat
     7021 ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca gcttctggaa
     7081 aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata ttccttttga
     7141 caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg aggcagacag
     7201 tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga
     7261 aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag agtatctggg
     7321 aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt caggtgcaag
     7381 ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat tacttcattc
     7441 tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt ctccaatcaa
     7501 aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa atcaattaat
     7561 catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata tgaaagacct
     7621 gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct gaaattcatc
     7681 taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat ggataaatta
     7741 caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg acatgggagc
     7801 gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga accgcaggca
     7861 gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga taaagtcgtt
     7921 aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt gccaatagta
     7981 gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc taaaaatctc
     8041 tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga tcgtatcgaa
     8101 gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca tgggcacccg
     8161 atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc
     8221 acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact
     8281 gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa
     8341 ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg gatcaaaaat
     8401 aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt cggatcattt
     8461 tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg ccgttcagct
     8521 ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat attgtaactt
     8581 gtgattcgga attggcagga gaacaccgtt tattcggaag aataggactg tgacaagact
     8641 tttgtcacag tccttttttc gaattcaaca agtaaaactt ttaagaaata ttcagagagt
     8701 aaaattaaaa attgctgata aacgaagatg gaaattttat gtatttttat tggtctatgc
     8761 aataaataaa ttaaatattc agaatacaat tgattttaac gaaagggctt ctatcaatgg
     8821 ttgtgttgta tttatctgcc caggtagagt aaaatagatc attatctaaa aggtaattgt
     8881 tcatactata taagtcattt gaaagtaaaa agatactgta aataaaaaat gtggttaaat
     8941 aattctcata atcagtgtaa gcgttatcaa aaatatagca tacttgcttt gtagatgaat
     9001 gacacgatat atgtttggta tgttctgcaa gacagatgaa tatggaggtg gagacaatgt
     9061 cttccaaaga actaacaatg tatttagaga aagaattgga tcaactgaaa gaaaaggggt
     9121 tatacaatac gatcgacata ctagaaagtg aaaatggtgc ttgtatcatc gtagacggaa
     9181 aaaagatgat caatctagca tccaacaatt atcttggatt tgccaatcgc gaagaattga
     9241 aaaaagcatg tattgaagca accgaaactt atggtgtagg agcaggagca gtccgaacga
     9301 tcaatggttc gctgaaaatc catcagcaat tagaagaaaa aatcgcagaa ttcaaaggga
     9361 cagaagcagc aatcgccttt caatctggat tcaactgcaa tatgggcgct atctctgcag
     9421 taatgacaaa agaagatgct atcttatcgg atgaattaaa ccatgcatct attattgacg
     9481 gttgccgctt atccggagca aaaatcatcc gaattaaaca tcaagatatg aaagaccttg
     9541 agaaaaaagc aaaggaagcg atagaaagta agaaatataa aaaaatcatg tatatcacag
     9601 atggtgtttt ctccatggat ggagacattg cacgattgcc ggaaattatt ccgattgttg
     9661 aaaaatatgg actgatcacc tacgtcgatg atgcgcacgg ctcaggcgta actggaaaag
     9721 gtgcaggaac cgtcaagcat tttggattaa gcgacaaaat cgatatgcaa atgggcacgt
     9781 tatcgaaagc tataggtgtt gttgggggtt atgtagctgg atcgaaaaca ctaatcgact
     9841 ggttgaaagc tagaagtcgg ccattcctat tttctacttc gctaactcca ggtgcagctg
     9901 cagctgcatt ggcttcgatt actttgatgc aagaacatcc ggaacttgtc gaaaaagttt
     9961 gggaaaatgc taattatttt aaagaagaat tgaaaaaagt aggctataac atcggtatgt
    10021 ctgaaacacc tattactcca gtcattttgg gagacgaaaa agtgactcag actttctcta
    10081 agaagctgat cgaacatggg atttatgcta aaccaatcgt ttatccaact gtgcctcttg
    10141 ggacaggcag gatcagaaat atgccgactg cggaacatac gagggaaatg cttgatgaag
    10201 cagtagcagt ttatcaaaaa ataggaaaag aaatggaaat tatttaacta aatttagttt
    10261 tttcgtctat ttaaaattag agaacatgtt cacgcatata ttgttaatca tctgaagata
    10321 gtagtaaaaa agatcaggga ggatcatcat gaaaaaagta ttggtgacag gatgtttagg
    10381 acaaattggt tcagagttga tcacacggct acgtcaagaa ctaggcagtg aaaatgtcat
    10441 tgcgacagat atacgcatgc ctgaagtcat tgagaaaaac gatatctttg agatcctaga
    10501 tgtaatggat tacgaaaaaa tgcgcagcat agcagaacaa tatcaagtgg acacattgat
    10561 tcatttggct gctcttttgt cagctgttgc agaagcaaaa ccacaattag cctggaattt
    10621 gaacgtgact gggctggtca atgcattgga agtcgctaga gaattccagc tgaaattttt
    10681 tacgccaagt tcaatcggcg cgtttggtcc aaatactcca aaagatcata ccccacaaga
    10741 tactcttcag cgtcctacaa ccatgtatgg ggtaacgaag gtagctggag aactactatg
    10801 tgattattat tatgtgaaat atggcgtaga tactcgtggt gtccgtttcc ctggattgat
    10861 ttcttatgca accttgccag gaggcggtac gacagactac gcagtcgata tttattattc
    10921 agcaatcaaa gaaggaaagt acactagttt cattgatcaa ggtacatcaa tggatatgat
    10981 gtatatgcca gatgcgatcg atgccattat aaaactcatg aatgcagatc ctgatcgatt
    11041 ggttcatcga aatgccttca atatatcggc gatgtctttt gacccggaag agatcaaagc
    11101 atctattcaa aaaatcatac cagattttga aatggattat gatgtggatc ctgtacgtca
    11161 gtcaatagca gaatcttggc caaattcact agatgtttct tgtgcgcaag aggaatgggg
    11221 cttttcgccg aaatacgact tagatgtgat gacaaaggat atgttggaaa aactgaaaga
    11281 aaaattggta gagacggtat aaaacaagaa ccacatccaa aaagtttttt atacttttgg
    11341 gattggctat ggtatgaaag gggacaaggg atggaagaat atcaagccac gccagtaaaa
    11401 gaagtaaaaa tagaaaatga attaaaacgt acgatgggat tttttactgc tttatcgact
    11461 gttatgggca cagtgatcgg tgcaggggta ttttttaaag ccgcaagcgt agcagaagtt
    11521 acaggatccg ccagtttgca tatgttttct tggtttctcg gtggtatgat ttctgtctgt
    11581 gcaggactaa caggagctga actggcagct gctattccag aaacaggagg gatgattaaa
    11641 tatatcgaac ggatttacgg aaatacagca gcttttttat tgggctgggc gcaagtcgtg
    11701 atttattttc cagcaaatgt agcagccttg tcgatcattt tcggtacaca gttcgtcaat
    11761 ctatttggtc tttcccagtc aatgattgtt cccgtagctg ttacagctgc tgtatcgatc
    11821 ctgttgatca attttttagg ttctaaagct ggaggggctt ttcagtcgat tacgcttgtc
    11881 tgcaagctta ttcctttgtt tgtgatcgtc attttcggtt tgttccgaca agaaggagta
    11941 gacttccaac tgtttccgat ccaagcagga gaaaatcttt catttttctc agccttggga
    12001 gcgggcttgc tagcaacgat gttcgcatac gacggttgga tacacgtagg aaatatttcg
    12061 ggagaattga aaaagccggc gaaagatttg ccaaaagcaa tttctttagg aatcatcgga
    12121 atcatgatcg tttatttatt agtaaatgcg gtgttcttaa gaaccgcatc tatcgacgga
    12181 gtcgcaggaa acagcaatgc ggcaagtgac gttgcaaaaa tgatttttgg cggcttcggt
    12241 ggaagactgg tcacagttgg tatcttgatt tctgtttatg gaaccatcaa tggctatacc
    12301 cttactggaa tgcgtctccc ttatgttatg gcaaaagaaa acaacctgcc ttttagcaag
    12361 ctctttgcta aacttcatga taaaactaaa gttcctgtag ccgcaggtat cttagagcta
    12421 gtcatcgcta ttggcatgat gatgatcggt ggttttgata cactaacgga tatgctgatc
    12481 tttgtcattt ggatttttta tacgatggtc tttgttggtg tcattcttct tcgtaaaaaa
    12541 gaacctgatt tgtttcgacc atacaaagtt ccaatgtatc cgtttattcc actagtagca
    12601 atcattggag ggacttttat cgtatcaagc acgcttatca ctcagacctt tttagcttcg
    12661 atggggatag tattgacact tgctggtatc ccgatttatc tctatttaaa acgtagatag
    12721 ctaaaacatt cgaattttat tagaactgca cttctctgcc aaacaaaata ttttatttaa
    12781 atgaggagta cgtggaagaa atgtcaaaag tactcgttaa ttttgatcac gtaaatgtcg
    12841 ttttgtctac gatgaaatag caaagtaaaa gacagaaatc gggctcaacc gatttctgtc
    12901 ttttactttg atttgcgtaa ctgaactatt cggcgtacgc cgcgataaat atttaataaa
    12961 accaagcaac cagaaatgaa aatgggaagc caaatcagat agacagggta gccaagcact
    13021 ttagacaaag catgtggagt cattttatcc atttcaagaa gtgcttgctg ccaagtctga
    13081 agattgaaga ctagcatgaa acccgaaata atcaggagaa taaatcctaa ataatgaatt
    13141 gtagttcttt ttcttacata agcgatcgta gcataaagga acagtaaaaa aataggtacg
    13201 ccaaaaacaa agtacatatt cagcatctcg acaccccctt tgtgtttatc ttactttact
    13261 ttgaaagaaa tgagaaataa tagcctcgtt acttgcttga aacagcaaag gactgctaag
    13321 ataagagaga tacaaagaat caaggaggag tcatatggca agttatatcg aaccgattcg
    13381 ttttgcgctg attgtttttc catttttagc cttagcgatc tcatctgttt tttttattta
    13441 tgaatatcga aagtatggaa catttctagt aacaagggca gttattttgt attctttcgt
    13501 tttttattta ttgtgtgcgt attttttggt gatcttacca cttccggcaa aggagacggt
    13561 tgcccaaatg acagggccaa agatggagct gcatttattt gcttcaaggc aacatttcag
    13621 agacaacacg gtattggtct tgtctgaacc tagtacctac ctgccggcaa tgaagcagag
    13681 tgtatttttg gaaccagctt ttaatgtttt attactcata ccatttggca tttatttaag
    13741 atattatttc cggctttctt ttatgaaaac gatgattgct agtttctgcc tttctctatt
    13801 ttttgaattg acgcagctaa gtggacttta ttttatctac ccaagaccat accgcttatt
    13861 tgatgtgaat gatttagtga caaatacatt gggtggaatc attggttttg tcgttacgcc
    13921 gttatttaca tttatgcttc ctacaagaag acgaatggat gaactttctt atgaaaaagg
    13981 aaagactgtc tcattgactc gacgcttggt cgcttatttg atcgactgga catttttatc
    14041 tcttattaat ttggtagcgg cgattttctt taaaattgcg ttcgattcag gggcagttac
    14101 ttctcatatc tggtggcttt tattagaagt aggggtatac tttatgattg taccgtttat
    14161 aaccaatgga tggacacttg gcaaaaaaat tgtgcgtatc cgtttggtgg aagaagaccg
    14221 cgaacgaatt tctttaaaag cattgatgat ccgctacggg tatctgtact ttctttttta
    14281 tggtttaagc tggttggggc agttgcttca gcaaatcatg aattcgacaa cgaatacggt
    14341 gttgcagatg agtgcagtat tcatttctct cggtgtcttg ttactacaag gtatttttat
    14401 tttaaatatc ttgctatcat ttttgagaaa aaaacggatt ctgttttatg aaaaagctag
    14461 tcacacatat actgttagca cgattggcca aacaaaggat gatgattctc gaaattagct
    14521 gagcaaaaac gctttgtctg tgttataatg ccaaagtcgt gataatttaa agagaagagg
    14581 tgcacgaatg aataattttg atatattccg cttcaaaccg tttatcaatg aagcgttaac
    14641 cgcaaagggc ttcaaacagc ccacagaggt ccaggaacgt ttgattcctt taattgcaaa
    14701 aggaaaaaat atcattgggc aatctcaaac tggtacagga aaaacacata cgtttttgtt
    14761 gcctttgttt gaaaaaatca atgcagaaaa agaggaagta caagttgtta ttacagctcc
    14821 aagtagggaa ctagctgcac agatctatga agcagctaca caaatcgcta aattttctac
    14881 accggagatc cgtgtctcga attttgtcgg cggtacagac aaacagcgtc aattaaatcg
    14941 tctgcatcac caacagccac aggttgtagt aggcacgcct ggtagaatat tagacatgat
    15001 gaatgaacaa gcgctgaaga ttcacaccgc atttgcattt gttgtcgatg aagcggatat
    15061 gacattggat atgggttttt taaatgaagt agaccaaatt gcaagtcgat taccagaaaa
    15121 gctgcagttt ttagtttttt ctgccactat tccagaaaaa cttcgtccat tcttacgaaa
    15181 atacatggaa aatccaatca ttgaagaaat caaaccaaaa gcagttattt ctgaagacat
    15241 tgaaaattgg ctgatttcaa caaaaggtaa agatgagaat caaatgattt atcaactgct
    15301 gacaattggt catccttatt tagcaatcgt atttgccaat acaaaacagc atgtggatga
    15361 aattgccgat tacttgaaaa accaaggctt gaaagtagca aagattcatg gagacatcac
    15421 cccaagagag agaaaacgag tgatgcgaca agtacagaat ctggagtatc aatatgttgt
    15481 agcgactgat ttagctgcta gagggatcga tatcgaaggt gtctctcatg ttattaatgc
    15541 agaaattcca gaagatctag acttctttat ccatcgtgtc ggacgtactg gacgtaatgg
    15601 cttaaaagga acggccatta cgttatattc gccaaatgat gagcaagcca ttgatgcgat
    15661 agaaaaattg ggcgtctcat ttgaaccaaa agaaatcaaa aacggggaaa tcgtcaaaac
    15721 atacgatcgt aatagacgtg ccaaacgaga aaaatcaaaa gatgtattag atcccacgct
    15781 gattggtctt gtgaagaaga aaaagaaaaa gatcaagcct ggttataaga aaaaaatcga
    15841 ttgggcaatt gcagaaaaaa ataaaaaaga acgcaaaatc gaacgcagac agcaaagccg
    15901 tactgctcgt aaaaataaga aaaattcaca taaataaaaa acaagaggtt gtgacaaaag
    15961 gcgtgtcaca atctcttgtt tttaagctag actttttttc attccgatac aaagtacttc
    16021 atcttcaata aaaggttctg tgcaagtatg atacccttgt ttttcataaa aatctacagc
    16081 cgtcatttca gctactacat aactagtctg acaaccttct gaaaccgccc gtttttcagc
    16141 agcttcaagc aactgaccgc ctaacccttt tcctcggtat tcttttgcta cgcaaaatcg
    16201 ttccggcttg aagcaagtag gtgttttctt ttcataacgc aaagtagcta ctggtctttt
    16261 ttgatcaaga aatagataat aggtttgttg cgggatatct tttccgtcga attctagttc
    16321 tgcaggaatc ccttgttcta atacaaaaac ttgatatctc aaaaagcagc tagctgcgcg
    16381 tatccattca tccgctccaa ctattttttt catcatattc ctccttgtca tgaatagcag
    16441 agatgtattg attcctttat tcttatcgta ccaaaaatcg tggagtatga ccaaaaggaa
    16501 atgaaaataa aaattgagat attgacagtg atcaatagaa tgcgtatact aattaagtac
    16561 gaaaaggata tgatttgttt tttaagactc acagaaaatt ctttcttggc tgaaaaaaga
    16621 atagtcacta atcaaattgc tccctttttc tattttggta gaaataccga cgcagctcag
    16681 cgttatcgag tttaagaggt aatgattaca catcattgca atcaaggtgg taccgcgagt
    16741 tttcgtcctt ggctgtgatg gtgttttttt gtatttaatt tatagaaagc aggtaatgac
    16801 atgaaacaat taagtagtgc acaagttcga caaatgtttc ttgatttctt ccaatctaaa
    16861 ggacatacga ttgaacctag tgcgtctttg atccccgtga acgatccaac cttattgtgg
    16921 atcaactctg gtgttgcaac attgaaaaaa tattttgatg gttcagttgt gccagaaaat
    16981 ccaagaatca caaatgccca aaaatctatt cgaacaaatg atatcgaaaa tgtcggtaaa
    17041 acagcacggc accatacgat gtttgagatg ctgggtaatt tttcgattgg tgactatttc
    17101 aaaaatgaag ccatccactg ggcatgggaa tttttgactg atccaaaatg gatggcattc
    17161 gatccagaaa aactttatgt aacagtttat ccaaaagata cagaagcaaa acgcatctgg
    17221 catgaagaag ttggcctgcc actagatcac attattgatg tagaagacaa cttctgggat
    17281 atcggtgcag gcccatgtgg tccggatacg gaaattttct atgaccgtgg agaagcttat
    17341 aacgatttag cagaagacga tcctgaaaac tatccaggag gagaaaatga acgttattta
    17401 gaaatctgga acttagtatt ttcagaattc aaccataagc ctgataacac atttgaacca
    17461 cttcctcata aaaacattga tacggggatg ggattggaac gcatggtctc aatcgttcaa
    17521 gatgcgtcaa cgaactttga gacagacttg tttatgccta ttatccacgc cgtagaaaag
    17581 ctaagcaaac aagtaaaata tggtgaaaac ccagtaacag atatttctta caaagtaatc
    17641 gcagatcata ttcgcgccct ttcatttgct attggcgacg gtgcattgcc atcgaatgaa
    17701 gggagagggt atgtcttacg ccgtttgctt cgtcgtgctg tcatgcatgg gaaaaaatta
    17761 ggtattgaac aagcattctt atatcgttta gtccctgttg ttggggaaat catggtaagt
    17821 tattatcctg aagtacttca acaaaaagaa tttattgaaa aagttatccg tacagaagaa
    17881 gaacgtttca atgaaacaat caatgaaggt ttggaaatct taaatcaagt gatccaagaa
    17941 gtaaaaaacg aaaacgggac gactctagca ggaaaagaca tcttcaaact atacgacaca
    18001 tatggcttcc cggtagaatt aacggaagag gtggcagaag atgaaggact gaaagttgat
    18061 catgctggtt ttgaaaaaga aatggatgca caacgcgaac gtgctcgctc tgcccgcagt
    18121 actgaaaaat caatgggggt acaatctgct ttattgacag atatcaaagt agaaagtaaa
    18181 ttcattggat acacagaaca agaatcagat agtcaattat tggtcatcgt taaaggtgag
    18241 tctcttttga atgaagtcgc agaaggtgaa gctcaaatga tctttgatca aacacctttc
    18301 tatgcagaaa tgggtgggca agtagcggat caaggatgga tcatgaatga agtaggcgaa
    18361 atcgtagcag ttgttacaga tgttcaaaaa gcgcctaatg gtcaatttat gcacaccgta
    18421 gaagtaaaag caccaatgag tgaaggacaa acctatcatc tattgatcga tcaatcgatg
    18481 cgcaatcgga tcatcaaaaa ccatacagca acccatctac tgcacaaggc attgaaagaa
    18541 gtacttggcg agcatgcaaa tcaagctggt tctttagtcg ctcctggaca tttacgattc
    18601 gactttaccc actttggtca aatcacttca gatgaattag ctgaaatgga aagaatcgtc
    18661 aacgaaaaaa tctgggaagc tatcccagtt gaaacaattg aaacagatat cgatacagcc
    18721 aaaaacatgg gtgcaatggc tttgtttggt gaaaaatacg gacatgacgt tcgagtagta
    18781 aatattggcg gctggtcaat cgaactgtgc ggaggcacac atgtagcaaa tacagaaaac
    18841 atcggaatct tcaaaatcgt ttctgaatca ggaattggtg caggcgtgcg acgaatcgag
    18901 gctgtaacaa gtaaagaagc gtacgaacta cttgaagaag aagaaaaaca actaaaacaa
    18961 gtagcaacaa ttgttaaatc acctcaattg aaagaagttg tttcaaaagc ggaacaattg
    19021 caacagcaag taagagagct acaaaaggaa aatgaacaat tagctagcaa gttagcgaat
    19081 caacaagctg gtgatgtatt caaagatcca gaaacgatca atggaaaaac aatcattgca
    19141 gcacaagtaa aagtgaaaga tatgaatcag ctgcgtcaat tagctgacca atggaaacaa
    19201 aaaaatattt cagatattct tgttttagca aacgaacaag aaggaaaagt cagtctgcta
    19261 gcggcaatga cgaaagaatc aaatgaagct ggactaaaag ctggcgactt gattaaagca
    19321 atcgcaccga aagtaggcgg tggcggtggt ggacgtccag acatggcaca agctggcggg
    19381 aaaaatcctg ctggtattcc agatgcactt aaagctgcga aagaatgggt tgcgcaacaa
    19441 tagggcgttt acgtttgaca gaaaaacaaa agttcaaaca ttgaacgaaa catgattatg
    19501 ggggcgaaga caaacaagtc ttgccctcat acttttttca attggatggc gcatctaatc
    19561 aaatggtcgg attcggatgg tctttagttg acgaaagtga cagaaatttt tacgattagt
    19621 ataaataaaa aggggtgaaa aaatgaatag tagaaataag acctttcgac tagtcttacg
    19681 tgcgatcttg ttagcaatca tcatcgtaca agcgatggtt ccttggctgg ggtttattcc
    19741 attaggattc attagtttaa caattatcca tatcacagta attattgctg cagtagtatt
    19801 aggcccaaaa gatggcatgg tgattggctt gttttgggga atcgctacaa ttgttcgtgc
    19861 ctatgccatg ccaacaacgc catttgatac attagtgttc acgaatccga ttatctcggt
    19921 agttcccaga gtacttgttg gcttagtagc aggcttggtt ttccactgga tttatcaaaa
    19981 aaataaatca ataacgatca gctcagcctt tgcaggggta ctgggttcat tggtcaatac
    20041 agtcttagtg ttaggattca tgggattatt ttatacaggt gcaacggcag atgcctatgg
    20101 tgtagactct gctcttttat ttaaaacctt agcaggggtt gcagcaatca atgggatacc
    20161 agaagcaata ggtgcgggaa tcataacacc attactagca aaagcgttat ttgcagcaac
    20221 accgttgaaa ccagaataat gaccatcttt taatagtaat aggacgcacg atagatagaa
    20281 aaaggaagag aggagaggaa aacaatcgtc ctctccatct ttttacctga gcaacaagca
    20341 aactagaagg ggtaaaaaac ttggctaaga aacaagaaga aaaaaagaaa caaataccgc
    20401 aacagcggac aaaaaaagga gtactagcgg gtatcttagg attggttacg aatctgatct
    20461 tgtttgtctc taagctaatg atcgggcttt tttcaggaag tgtctccatt atggctgatg
    20521 cagtgaacag tctatcagat actgcttcat ccatattgac tttgatcgga tttaaaattg
    20581 ctgcaaagcc agcagatcag gaacatcctt atggacacga acgcttcgaa tatatcagtg
    20641 ggttatttgt ttcgatcatc attacttatg taggatttca atttttagag tcttctgctg
    20701 aaaaaatcat ccatccagaa aatgtctctt taacacccat cgtctttttt gtcctaatca
    20761 tttccatctt tttgaaattt gcacaaggca aaatgtatca ggttattgcg caatccattg
    20821 attctgaaac attgagagct acagcaacag atagttataa tgatgtcttt actacactgg
    20881 ctgtattgct ttcagcagca atcgaatggg taactggatg gcgtattgat ggatatatag
    20941 gttttgcttt agcattatat atcctttata gtggattgac gatgcttcga aattttgtat
    21001 acgaactatt aggaagtcgc ccaacgccag cagagattga agaaatggaa gagcatttaa
    21061 atgcttgcga tgatatactt ggctatcatg atttattggt gcataactat ggacccaata
    21121 aacgctttgc atcagtccac atcgaagtag atgaatcaat gaatttgaat gtggctcata
    21181 agattattga tctgatagaa aaagacttta aaaagtcttt ggatgtggaa cttgtctgtc
    21241 acttggatcc aatgcctgtt gataacgaaa aatactataa tatcttgcgg cagttaaagc
    21301 aaattcttct tgaaacaggt acagggctta atattcatga ttttagagta attcatcaag
    21361 gaaaaacgct gcaatttgat gtagtggtcc ctgagaaatt ccagtttgtt gatacagaac
    21421 tagaaagaat cttacgtagt caaatagaag aaaaaatcgg agattac
//
LOCUS       DAXWLH010000038        21270 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXWLH010000038 DAXWLH010000000
VERSION     DAXWLH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.038"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            80..2245
                     /locus_tag="ACGE36_001034"
     CDS             80..2245
                     /locus_tag="ACGE36_001034"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFF8383448.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2549..3382
                     /locus_tag="ACGE36_001035"
     CDS             2549..3382
                     /locus_tag="ACGE36_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFF8383449.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3484..5184)
                     /locus_tag="ACGE36_001036"
     CDS             complement(3484..5184)
                     /locus_tag="ACGE36_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383450.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            5691..6851
                     /locus_tag="ACGE36_001037"
     CDS             5691..6851
                     /locus_tag="ACGE36_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8383451.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6867..7310
                     /locus_tag="ACGE36_001038"
     CDS             6867..7310
                     /locus_tag="ACGE36_001038"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383452.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(7793..8323)
                     /locus_tag="ACGE36_001039"
     CDS             complement(7793..8323)
                     /locus_tag="ACGE36_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFF8383453.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            8428..8979
                     /locus_tag="ACGE36_001040"
     CDS             8428..8979
                     /locus_tag="ACGE36_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8383454.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(9132..9605)
                     /locus_tag="ACGE36_001041"
     CDS             complement(9132..9605)
                     /locus_tag="ACGE36_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8383455.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            9743..11215
                     /locus_tag="ACGE36_001042"
     CDS             9743..11215
                     /locus_tag="ACGE36_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8383456.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            11212..11922
                     /locus_tag="ACGE36_001043"
     CDS             11212..11922
                     /locus_tag="ACGE36_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFF8383457.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            12274..12558
                     /locus_tag="ACGE36_001044"
     CDS             12274..12558
                     /locus_tag="ACGE36_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFF8383458.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(12839..13258)
                     /locus_tag="ACGE36_001045"
     CDS             complement(12839..13258)
                     /locus_tag="ACGE36_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFF8383459.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(13260..13844)
                     /locus_tag="ACGE36_001046"
     CDS             complement(13260..13844)
                     /locus_tag="ACGE36_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFF8383460.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(13891..15366)
                     /locus_tag="ACGE36_001047"
     CDS             complement(13891..15366)
                     /locus_tag="ACGE36_001047"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFF8383461.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            15547..15858
                     /locus_tag="ACGE36_001048"
     CDS             15547..15858
                     /locus_tag="ACGE36_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383462.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(15974..18310)
                     /locus_tag="ACGE36_001049"
     CDS             complement(15974..18310)
                     /locus_tag="ACGE36_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFF8383463.1"
                     /translation="MLQLKDIKKYYKVGEPTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            18452..19120
                     /locus_tag="ACGE36_001050"
     CDS             18452..19120
                     /locus_tag="ACGE36_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8383464.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            19123..20166
                     /locus_tag="ACGE36_001051"
     CDS             19123..20166
                     /locus_tag="ACGE36_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8383465.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(20403..>21270)
                     /locus_tag="ACGE36_001052"
     CDS             complement(20403..>21270)
                     /locus_tag="ACGE36_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8383466.1"
                     /translation="FEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELDVKLFE
                     RAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMINYKGF
                     SKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWLPTETD
                     DFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPKISYKG
                     ARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGEHSLAS
                     DTFWEYLNQEEN"
BASE COUNT         6693 a         3763 c         4337 g         6477 t
ORIGIN      
        1 aaaataaagc atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg
       61 cgtaaaggag ctaaacggaa tgaaaaagat atttagtttt gagttttggc aaaaatttgg
      121 aaaggcttta atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg
      181 gaaatcaatt cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat
      241 tgaaaatatt ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg
      301 aggaagttgg gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt
      361 aatcaatcgg atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga
      421 tgcatttacc cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt
      481 attggaagca ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg
      541 ggcaatggct tataataagt actataatta tagaaaattg ccggatgctt tgtcattttt
      601 caatggaaag cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat
      661 tcttgctgtg gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc
      721 agaatcacaa gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt
      781 actattgcca tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg
      841 cggatcgtat gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc
      901 gttatggttg gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca
      961 atatgagttt gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg
     1021 ttcttcaggt atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa
     1081 gaaagcaaga tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt
     1141 aacagaacca ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc
     1201 agtgatccaa ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt
     1261 tgggaatatc gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga
     1321 tcttatcaac ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa
     1381 tttcttgatc aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga
     1441 cagtgaagaa accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca
     1501 gatcattcat ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg
     1561 gttacgcgta agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc
     1621 aggggcgatg ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc
     1681 agatgtcttg aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc
     1741 agaaatggtt tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca
     1801 agaactagtt tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt
     1861 attctcacaa aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt
     1921 tgcgccgatc gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga
     1981 aacgaaagat ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca
     2041 gccagctttt gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc
     2101 tgagatggat cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt
     2161 tacaaacgat aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa
     2221 agtgatcggc tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc
     2281 ataagaacag ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg
     2341 agaagcagca gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa
     2401 gtgataaaaa cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt
     2461 tagcaggaga acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg
     2521 tttcgaaatg aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg
     2581 gcttgagcca gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga
     2641 ttatgaagtg attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc
     2701 tgcgaagcta atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt
     2761 tgcctaccgt ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga
     2821 aatgagccac atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac
     2881 attggaaagt caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg
     2941 caagatcttg attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca
     3001 cttttcttgg tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa
     3061 ctactttttg gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga
     3121 ctctcaaggt tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt
     3181 cgcgaatgaa aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa
     3241 tacagaaaaa ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata
     3301 tcaacggatt tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat
     3361 cgaattagat gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata
     3421 aaactctcca aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt
     3481 tgcctaagca tgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca
     3541 gccaatcaaa gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt
     3601 gagctcgttg attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata
     3661 atcagtcaat gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat
     3721 atgaacagtt ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt
     3781 ttttataaaa gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc
     3841 ttttgtctcc ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct
     3901 ggtagacata tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata
     3961 agtatgtcga ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg
     4021 gatcgtctgt gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg
     4081 ttcagaccac tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac
     4141 gaaaaatcga aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt
     4201 cgtccatgga atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat
     4261 tttttggaat aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat
     4321 caatatagga aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt
     4381 ttgactcatt tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc
     4441 taatcctgcc caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg
     4501 aagcagtgct tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata
     4561 catcaaagct aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac
     4621 agcgactttg ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca
     4681 taaaggataa aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat
     4741 agaatacgta ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac
     4801 atatctcagt aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag
     4861 aatctgataa tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa
     4921 aaatgggccg taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg
     4981 aaaataatgg aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg
     5041 gaatggggca tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc
     5101 caaagcaatg aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt
     5161 gcaaacttta gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa
     5221 ttcatgaaaa tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag
     5281 gatgcgacat aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg
     5341 aaacaaagta gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt
     5401 aataagctca ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact
     5461 caaagcataa cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg
     5521 cggtaataag cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt
     5581 acttgatgca ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg
     5641 aacccatata ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga
     5701 gtaagcaaaa acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg
     5761 tagggttact gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc
     5821 aaaaacgtat cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga
     5881 aaatggagga gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt
     5941 tcaatgaaaa gaacagaaat gaaagtacaa catccagcca attagaggaa tggctgatcg
     6001 gaagtatcaa tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta
     6061 ggatccttga aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt
     6121 cgacgcattc agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat
     6181 tagatcgttt agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt
     6241 atcaagttaa aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag
     6301 aaggagaaga cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt
     6361 tgctagtgac aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag
     6421 gaaatcagga aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag
     6481 tcatactgtt agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc
     6541 ttgatttttc atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca
     6601 ttttgaaaca taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg
     6661 accatcatgg gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag
     6721 ctgatgatcc taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat
     6781 acttttttga ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa
     6841 tcaatgaata agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag
     6901 aactggaatc tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc
     6961 catcaactta ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga
     7021 tttacttagc agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta
     7081 tcgcaggaat ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga
     7141 aaaaaataaa agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag
     7201 ctattcattt ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag
     7261 taaatgagga ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat
     7321 aacgaaataa tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac
     7381 taatttaact gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct
     7441 gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc
     7501 tctaatactc aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc
     7561 tgcttctcgg taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg
     7621 ttcttattac tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca
     7681 gctgaccctc ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct
     7741 ttctctaata ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta
     7801 ctcgtttata attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt
     7861 atggttcttc gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg
     7921 tagcaaattc aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca
     7981 tttctgcgat atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt
     8041 tagggaaaaa gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt
     8101 ctgtccctgt tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat
     8161 cagcttcttg gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa
     8221 cagtatggtc atttttggca taccattcat attcccaacc attgtcatac gtatagataa
     8281 agtgagttcc aataaaatct tccaattctt taaattcttt catgtttaag catctcctta
     8341 taaattaaaa catgtaataa actacatgtt ttcgattact catttatgat agaatggatt
     8401 taaagatttg tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg
     8461 ttagggttat taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat
     8521 gaaataggag aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg
     8581 gaagagcaag gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa
     8641 gtttatgaat taacagaaga agggaaggaa caattggcag agtggattca tacacccacc
     8701 aatgaactac cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg
     8761 aatgatccgg cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga
     8821 cttcatctat tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat
     8881 tacggacact acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg
     8941 ctaacagatg tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg
     9001 gagaattcct ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa
     9061 caggcctttt ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag
     9121 attagtggcg tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga
     9181 aggagcttgt aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc
     9241 tcttctttga agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct
     9301 ttgattgctt ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc
     9361 ttttctgcag cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact
     9421 tttatgactg taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga
     9481 atgaatccct tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt
     9541 aattcactga tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca
     9601 accatttttg tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat
     9661 gtgtcaagta ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa
     9721 aaattcagaa agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc
     9781 atataatcgg acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa
     9841 tcagacctta ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc
     9901 tgatgtgcaa tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac
     9961 agcatggctg atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt
    10021 tttgatcgga acgattactt gctttacagc acctaatttt agtacattac taatcggtcg
    10081 tttgatccaa gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc
    10141 tattttcccg ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact
    10201 tgctcctgct ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga
    10261 cttgtttggt atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat
    10321 gaaaagtgtt attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac
    10381 gattggattc ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag
    10441 cttgaaagta attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg
    10501 tcaattacat ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac
    10561 gattgcagct gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt
    10621 accattgtat attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt
    10681 gccgggtgca ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata
    10741 cggcgcgaaa cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt
    10801 tgctttcttg acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat
    10861 gtttgggatc tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa
    10921 taacttgatc agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat
    10981 tggtacagct attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa
    11041 atctgtattg gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc
    11101 aggttaccat gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc
    11161 ctttttattg aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt
    11221 ggagttttga ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca
    11281 tggcaaacct ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc
    11341 gtcgctaatt tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa
    11401 attgtttcca gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat
    11461 ggaaaagaaa aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact
    11521 ggtacggata acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg
    11581 aaaactactt attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat
    11641 aacaatcacg aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa
    11701 ttaagtacgg accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg
    11761 gaaagtgaag caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca
    11821 aaaatgagca aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa
    11881 caagcattcg caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta
    11941 ggaggttgtg aaaaagctgc tctgcatcaa gcagtaagaa caatcagata aaaatagcac
    12001 tccatatttt tcatctgatt gggcttagtg ccgtagatgc aagcttttga acaccgttta
    12061 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
    12121 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
    12181 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
    12241 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
    12301 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
    12361 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
    12421 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
    12481 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
    12541 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
    12601 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
    12661 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
    12721 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
    12781 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
    12841 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
    12901 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
    12961 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
    13021 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
    13081 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
    13141 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
    13201 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
    13261 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
    13321 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
    13381 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
    13441 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
    13501 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
    13561 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
    13621 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
    13681 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
    13741 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
    13801 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
    13861 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
    13921 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
    13981 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
    14041 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
    14101 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
    14161 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
    14221 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
    14281 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
    14341 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
    14401 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
    14461 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
    14521 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
    14581 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
    14641 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
    14701 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
    14761 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
    14821 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
    14881 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
    14941 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
    15001 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
    15061 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
    15121 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
    15181 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
    15241 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
    15301 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
    15361 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
    15421 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
    15481 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
    15541 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
    15601 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
    15661 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
    15721 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
    15781 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
    15841 agtataccag aattataaaa aataattaaa aaaaaacagc gcgaaacaag gaacatatta
    15901 cttcctcgtt tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt
    15961 ggttgtcaca accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc
    16021 gtgcaggaag atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg
    16081 catggacagg atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat
    16141 tgatagggaa cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta
    16201 aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt
    16261 ttagtacacc gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca
    16321 tgatcatact tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat
    16381 ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat
    16441 cttcatctga tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt
    16501 caaaattgtt cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa
    16561 gcaatgtttg tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt
    16621 ctctttgagc tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt
    16681 gatcgctata agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta
    16741 ggatccccga gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat
    16801 tagggataaa attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga
    16861 atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa
    16921 gattcttcaa tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg
    16981 ttgctgaggc tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt
    17041 tgctagtgtc aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg
    17101 tactagataa agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta
    17161 ctttgccatt tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat
    17221 tacttaagtt aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg
    17281 tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat
    17341 ccgctttgct tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa
    17401 tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc
    17461 cagaagaaag ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg
    17521 tcaagaaagt acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc
    17581 aaaaactcat ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag
    17641 gatgggaatc tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat
    17701 gggccagttc aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga
    17761 tcaattccat gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca
    17821 aaaggatatc tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac
    17881 cagataactg atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat
    17941 cttctgcccg ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa
    18001 cgttttcaat aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag
    18061 agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga
    18121 ttaccatatc accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt
    18181 taccagaacc actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca
    18241 caccatccaa tgctttggtg gtgggttcgc cgactttata atattttttt atatctttta
    18301 attgaagcat aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca
    18361 gaacaatgat cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc
    18421 tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga
    18481 tccgcaatta cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac
    18541 cgcattgaat ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat
    18601 tacagatatc atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac
    18661 cagatttgac acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg
    18721 cgggttcaaa ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat
    18781 cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat
    18841 cggacaaaca aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat
    18901 tctgcctcaa aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt
    18961 cactcgccaa cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac
    19021 gatcgatgta catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat
    19081 catcacagta cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt
    19141 tcacaattat ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg
    19201 tgttttctcg gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct
    19261 ggaaaaccaa cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca
    19321 ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat
    19381 atgagtctgg tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca
    19441 gaagtacagc aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag
    19501 acactgagga atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca
    19561 atccaaggat atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata
    19621 tttttatatc ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta
    19681 aaattgaaca aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat
    19741 gaacaaatca gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa
    19801 ttggatatcg aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg
    19861 tggctgaata tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc
    19921 aaactatttg agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg
    19981 aatgaagaga ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa
    20041 atttctggga atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga
    20101 cgtattgatt atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa
    20161 caataggaaa aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt
    20221 cgtttttttg tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt
    20281 cttattgaac tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag
    20341 agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg
    20401 gcttagtttt cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca
    20461 ccgactttac gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca
    20521 gtattttctt ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca
    20581 tttgcaatca tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca
    20641 aatccagctt cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg
    20701 agcaaaaagt tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga
    20761 tgatttttag gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa
    20821 cttggtgggg cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca
    20881 ttgtaagtta attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt
    20941 ttcaaaaact cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag
    21001 atatttaatg aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg
    21061 tattgttcta cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg
    21121 atttttctat gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc
    21181 tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt
    21241 gtttcggcta aatcaagaaa aacttcgaat
//
LOCUS       DAXWLH010000039        21231 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXWLH010000039 DAXWLH010000000
VERSION     DAXWLH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.039"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(126..1175)
                     /gene="recA"
                     /locus_tag="ACGE36_001053"
     CDS             complement(126..1175)
                     /gene="recA"
                     /locus_tag="ACGE36_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFF8383467.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1284..2525)
                     /locus_tag="ACGE36_001054"
     CDS             complement(1284..2525)
                     /locus_tag="ACGE36_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFF8383468.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2765..3343)
                     /gene="pgsA"
                     /locus_tag="ACGE36_001055"
     CDS             complement(2765..3343)
                     /gene="pgsA"
                     /locus_tag="ACGE36_001055"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFF8383469.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3552..4475)
                     /locus_tag="ACGE36_001056"
     CDS             complement(3552..4475)
                     /locus_tag="ACGE36_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFF8383470.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5214..6500)
                     /locus_tag="ACGE36_001057"
     CDS             complement(5214..6500)
                     /locus_tag="ACGE36_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8383471.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6493..7761)
                     /locus_tag="ACGE36_001058"
     CDS             complement(6493..7761)
                     /locus_tag="ACGE36_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8383472.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8013..8474
                     /gene="mscL"
                     /locus_tag="ACGE36_001059"
     CDS             8013..8474
                     /gene="mscL"
                     /locus_tag="ACGE36_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFF8383473.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8533..8979)
                     /locus_tag="ACGE36_001060"
     CDS             complement(8533..8979)
                     /locus_tag="ACGE36_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383474.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9072..9317)
                     /locus_tag="ACGE36_001061"
     CDS             complement(9072..9317)
                     /locus_tag="ACGE36_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFF8383475.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9432..10010)
                     /locus_tag="ACGE36_001062"
     CDS             complement(9432..10010)
                     /locus_tag="ACGE36_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8383476.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10087..10617)
                     /locus_tag="ACGE36_001063"
     CDS             complement(10087..10617)
                     /locus_tag="ACGE36_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFF8383477.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10621..11481)
                     /gene="rlmB"
                     /locus_tag="ACGE36_001064"
     CDS             complement(10621..11481)
                     /gene="rlmB"
                     /locus_tag="ACGE36_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFF8383478.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11474..11869)
                     /locus_tag="ACGE36_001065"
     CDS             complement(11474..11869)
                     /locus_tag="ACGE36_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFF8383479.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11866..13278)
                     /gene="cysS"
                     /locus_tag="ACGE36_001066"
     CDS             complement(11866..13278)
                     /gene="cysS"
                     /locus_tag="ACGE36_001066"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFF8383480.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13275..13811)
                     /gene="epsC"
                     /locus_tag="ACGE36_001067"
     CDS             complement(13275..13811)
                     /gene="epsC"
                     /locus_tag="ACGE36_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFF8383481.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13897..14136)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14133..15593)
                     /gene="gltX"
                     /locus_tag="ACGE36_001068"
     CDS             complement(14133..15593)
                     /gene="gltX"
                     /locus_tag="ACGE36_001068"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFF8383482.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15763..16920)
                     /locus_tag="ACGE36_001069"
     CDS             complement(15763..16920)
                     /locus_tag="ACGE36_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFF8383483.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16954..18324)
                     /gene="radA"
                     /locus_tag="ACGE36_001070"
     CDS             complement(16954..18324)
                     /gene="radA"
                     /locus_tag="ACGE36_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFF8383484.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18387..18914)
                     /locus_tag="ACGE36_001071"
     CDS             complement(18387..18914)
                     /locus_tag="ACGE36_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8383485.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19144..19950
                     /gene="proB"
                     /locus_tag="ACGE36_001072"
     CDS             19144..19950
                     /gene="proB"
                     /locus_tag="ACGE36_001072"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFF8383486.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19947..21194
                     /locus_tag="ACGE36_001073"
     CDS             19947..21194
                     /locus_tag="ACGE36_001073"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFF8383487.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
BASE COUNT         5749 a         4620 c         3885 g         6977 t
ORIGIN      
        1 atatttttat tttatgtacc cactcgtata attaaataac tcacgatttc tttattcaat
       61 aaaaaaacgg caggtcaagg agagtaccta ctcttcagac tagccgtttt tgtttatcat
      121 aatttttatt cttctactaa aggcaattct tcttgtcctt tttcatcttc tgggacagcg
      181 acttcttcac caataccata agctgctcta actagagcag aaacttctgc catcatttca
      241 ggatgattag ccatatagat tttagcgttt tcccgtcctt gaccaatccg atcttcttta
      301 taagaatacc aagcaccgct tttatcaaca atgtcttttt ctactgccat gtctagaagt
      361 tctccttctt gtgagatccc taaaccatac atgacatcta cttcagcgat tttgaaagga
      421 ggtgctactt tgttttttac gactttgatc tttgtacggt taccaacaat atctgtacct
      481 tgtttcaatt gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg
      541 cgtcctccag gagtgatttc tggattccca aacatcacgc ctactttttc acggatttgg
      601 ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac cagagagttt acgcaatgct
      661 tgagacatca aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc gtcgatttct
      721 gctcgtggaa ctaaagcagc aactgaatcg actactacga tatctacagc cccacttgat
      781 actaaagcat cagcgatctc tagaccttgt tctcctgtgt caggctgtga aagaagtagt
      841 tcatcgatat tcacacctaa tttttgtgca tattgcggat ctaacgcatg ctcagcatca
      901 atgaaagcgg ccgttccgcc atttttttgt acttctgcaa tagcgtgtag tgcaactgtt
      961 gttttacctg aactttcagg tccatatact tcgatgatac gtccacgggg gtaaccgccg
     1021 acacctaaag cgacatctaa cgcaagagag ccacttggaa ttgtagagat ttgttggtcg
     1081 attttttcgc ccaatttcat aatagagcct ttaccatagc tcttttcaat ttttttcagt
     1141 gcagcatcta aggctgcttt acgatcatct gccaatacga taatcctcct tatatttaca
     1201 aatcagagca acatttcatt atccttatca tatctttttt agtgaaaaaa agcaagaaaa
     1261 aaccgaacaa atattcgcat ttatcatttt cttaaaagct gttttctcaa aagatcaaat
     1321 ccttgcataa ccgcattata tctgatagca gcacgatctc tggcaaagtg atatttttcc
     1381 gaaaccacgc cgtctttaga tgaaatagcg atccatactg ttcctgcaga ttgaccttct
     1441 aacgtgtccg gtccagccac tccggtgaaa gctacactgt aatctgttct tgctatctca
     1501 cgtgcttgaa tggccatctg ctccacacat tctttgctga ctgttccata ttctgctaat
     1561 agctccggat caatctccag aaaaccagct tttgtttgga gcgagtaggt aacgaatccg
     1621 cctggaaata cttcagatac accagcaatg tcgcctaatg cggcttggaa ggcgccggct
     1681 gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt tcagtagttc cactacgact
     1741 tttgctagcg aattatcatc gccgtatcca taaaaatatt cccctactcg ttcttggatt
     1801 ttttcttcta atgctaaaag agcttgattc cctgcctgaa catcatttgt tttcactgtc
     1861 agtcgtaatg tcacttcatt tggtttggca tatggtgcga tcgttggatt gatctgtgtc
     1921 tcaatcagat cttttagctc agtgaccagt cgcgattcac caatcccata aaagcgcaaa
     1981 actttagaaa ttagtttttc ttcagaagga aaacgttcct ctagtaaagg acgtacttgt
     2041 tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa gaaggatata cgcatgtgta
     2101 tctgtttgat aaaaagtccc taaagcaagc cctgtacgat ttggtagagg aaccccgcct
     2161 tcgataatct gggactgttg cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa
     2221 tatgccaata attttttgta tccttccgta ttttgtatga gagacttacc gaggtgagcc
     2281 gcagttgctt cttttgtcag gtcatcttct gtcggtccta atccgccaca taagagtatg
     2341 agttcacttc ttgtttcagc tagagcaatc aactcttcta aacgctgctg attatctcct
     2401 acaactgttt gataataaac ttcgatacct agatcagcta gctgttctga caagaaagtg
     2461 gcattcgtat tcacgacttg tcctagtaaa agttctgttc cgacagctat tatttctgct
     2521 ttcattactt cccgctccct tatatagtat acgctgtatt tttatatttt tttatttaaa
     2581 aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa ataaacagtg
     2641 ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa aagctgtttt
     2701 ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct ccactaaacg
     2761 atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc acacctgaat
     2821 agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata ccgatcaggc
     2881 tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta gccgtcttga
     2941 cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt aaacgcaagc
     3001 ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg gcctttcctt
     3061 ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac gggtccgcaa
     3121 acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct aaccagtctg
     3181 tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc agtgtggtgt
     3241 ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg aaaatcggga
     3301 tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg actcctttct
     3361 tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt ttccaaatga
     3421 atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga agaaggaccg
     3481 cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga tgcgtaaact
     3541 aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg actctgattg
     3601 ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc attgcttgct
     3661 gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact ctgtgtttca
     3721 tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc taaccataca
     3781 cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat atttactgcg
     3841 atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc acttgatttt
     3901 gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga ggaagaggac
     3961 tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat tggatcagct
     4021 tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc aaccaatcct
     4081 aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc ctgatgcatt
     4141 gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga tcctgtcaat
     4201 ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac agcttcagcg
     4261 tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa atcatcattg
     4321 tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc atctaaagat
     4381 atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa agccactcgt
     4441 tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga aagacgttat
     4501 gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa acattgttct
     4561 cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc tgttttttga
     4621 acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct taaacggtgt
     4681 tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga gatatttttc
     4741 cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc cttaaacggt
     4801 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt aagaagctgt
     4861 ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttggtaca
     4921 cggtgttcca gaagtagctt cttcggcaat aagccgaaat atcacaaaaa tttgagaagc
     4981 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctctttga
     5041 ttacttcgtt tgattttatt ttaatataag ttgattaaaa gagagcatga tttgttttat
     5101 tttctcacta atttttccaa cacaactaag aataccataa aagctgattt tggaggaggt
     5161 tcctactaca agtctaaaac gaaatttaag acttttcctc ataaaatact ggtttatttt
     5221 ggtctcatgt aaaactcgct gaaggcctct tcacttataa atgcttcccc agctgccagt
     5281 acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt tcgatcgcca
     5341 aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga ttggaaatat
     5401 tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc cggatcatca
     5461 gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc gtccgtatct
     5521 ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc aaagctgtca
     5581 tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc taaaatcatt
     5641 tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc ttgcggcaat
     5701 gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc ttccaatgca
     5761 ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc ttgctttgga
     5821 gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc aggctccatc
     5881 ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata gcacgtatac
     5941 aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc gatatgcaat
     6001 gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt agggtcgtct
     6061 tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt ttcttctgtg
     6121 aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac ctgatccgtc
     6181 gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga agctccttgt
     6241 ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg ttctaaaaaa
     6301 tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat aaattcgtta
     6361 tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc atttttaggc
     6421 aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc attgatttgc
     6481 tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg cctgcaattc
     6541 gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac gtttcaacca
     6601 ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag cttcgattgc
     6661 cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt ttgtctgaga
     6721 cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt gaatcagatg
     6781 aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga atcctcggaa
     6841 cgtatccacg ctactagagg cataataagc tagactttct ttctctctga cattcatgaa
     6901 aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg caaaacgttc
     6961 tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt gaacgattgg
     7021 ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa acagatctgg
     7081 ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg ctttgtccgg
     7141 atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt cttgatagta
     7201 ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca accctgggat
     7261 cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag caaaagtttg
     7321 tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca agtcaaacgt
     7381 caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt ttaaaaaatc
     7441 taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa aactgatccc
     7501 tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc ttgcgccata
     7561 aagctctgca agttgcgcac ttaactttgt ctgatccgga taattcaaac tatttgtttc
     7621 caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac gcgcagtaaa
     7681 acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa gattcacgcc
     7741 ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg cccgcttact
     7801 cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt ataagttcag
     7861 gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag atgtttttta
     7921 tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt tttcgatata
     7981 ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa gagttcatca
     8041 tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg tttacaagca
     8101 tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt gtcgcaatga
     8161 tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg ccgacaaaag
     8221 gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg atcactggtt
     8281 ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc tccaaagaag
     8341 agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac atacgcgatc
     8401 ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca aatctttccg
     8461 atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg tcggctttct
     8521 ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg gaatatcatc
     8581 ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag cttgtaggta
     8641 ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca tgttaatcgt
     8701 ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa caaggacacg
     8761 tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga tcttcccatc
     8821 agtatcttga aaaaacgtat gccaagcttg tccatcaatg tcatcaactt cttgataagg
     8881 acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt aaaactctgc
     8941 tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc cccctgcttt
     9001 tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg aatctcaggc
     9061 tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg agtaagtaca
     9121 tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa atcaacaacg
     9181 aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg ctttcttcct
     9241 gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt cttgattgaa
     9301 gctaaagttg ttggcataca gttcacctct actatcaact ataccacata tttttaaaat
     9361 tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa gctcattaag
     9421 atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt gagttttact
     9481 ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc catgtctaca
     9541 aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga aaactggatt
     9601 tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg ttcttgcaat
     9661 gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat tgagacttca
     9721 tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata gatttgattg
     9781 cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc gtaagttctc
     9841 aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac ataatttctt
     9901 aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata cagggcagca
     9961 aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat aaaataattc
    10021 ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac tgattagaac
    10081 gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt tttttaactg
    10141 ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt tattttcttg
    10201 ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag aagttcgtaa
    10261 tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg
    10321 tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag ccgtttcgtc
    10381 ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga tgcccggaac
    10441 tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat aatttgataa
    10501 acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa gctggacaag
    10561 ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt ttttcatggt
    10621 ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag caacactggc
    10681 atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca tttctttttt
    10741 caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg atcctttggc
    10801 attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa atcctgtttc
    10861 tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac
    10921 tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt gttttggtat
    10981 gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg
    11041 atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc
    11101 aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga ctccttgatg
    11161 cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga ctggcacagc
    11221 ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat cttcttgaag
    11281 aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt gcccaaagac
    11341 gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc gtccagtttg
    11401 ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg attgatgttt
    11461 gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag cggatcaatt
    11521 cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct tcgaaccctg
    11581 ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt ttagcatttc
    11641 ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc agcatttgct
    11701 gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga tgcagcttat
    11761 tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg taaatcgcat
    11821 ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg cttctcttct
    11881 ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca acaaatcacg
    11941 aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa
    12001 ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga agatcgaaag
    12061 ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt tcggctggtc
    12121 tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat ttgctgcatt
    12181 aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt
    12241 atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca aattgtcaaa
    12301 cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt cactgtagcg
    12361 gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt
    12421 tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt tttcatcgtc
    12481 ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt tccctgtttt
    12541 cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt gcccgccacc
    12601 atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt caatatgcca
    12661 accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt
    12721 ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg tacgctgact
    12781 tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat tttcaaattt
    12841 acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt caaccaacac
    12901 ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca gtgtagcagg
    12961 ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat cagcaacttc
    13021 tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta catccgtgaa
    13081 attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa
    13141 agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg gtccgcagac
    13201 atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc tggtcaatgt
    13261 attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg aacgactttt
    13321 gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac ggcacctgca
    13381 ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt cgcagagagc
    13441 atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt gccgcctaat
    13501 gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt
    13561 cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg gatctcaaca
    13621 ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag ccggtgctga
    13681 tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg
    13741 atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat cgctcgggta
    13801 aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat catcttctta
    13861 taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt ccgcgtggtt
    13921 ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat acgttcttgg
    13981 ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa gaccactccc
    14041 agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc tcttgtaatg
    14101 atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt taaatgtgct
    14161 aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc atgcatttgt
    14221 cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc agtttctttt
    14281 tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat ttgttctagc
    14341 tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac ttcttttgct
    14401 gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac gatttctgca
    14461 gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc gattgtcttt
    14521 tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca catgtcagta
    14581 aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag ttttttcgca
    14641 tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat caattcttgg
    14701 tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat aaagttgaac
    14761 attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa gattgattcg
    14821 tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat atgtccgaag
    14881 gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt attggcaata
    14941 tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca
    15001 aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc gatattatcg
    15061 gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt attgcgaggc
    15121 acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg ttcttcaggt
    15181 gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc
    15241 cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc
    15301 agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta agggccgtat
    15361 tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag gttttccaac
    15421 tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat acgaataata
    15481 aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct tgcatttcca
    15541 atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt cattttcttc
    15601 ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa
    15661 atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta aatagtaaag
    15721 ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg attgtttagt
    15781 ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa
    15841 gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga tacgttcatt
    15901 catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt aggctactcc
    15961 ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac cagggatgac
    16021 gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat tttggaattc
    16081 agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa gcttagccaa
    16141 tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt acatttcgac
    16201 agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac gaccacgcac
    16261 acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa caaaattagg
    16321 gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc gaccgtcaat
    16381 gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt ttcgttctaa
    16441 cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa
    16501 aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat agcctaagat
    16561 gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat tgtaaagagg
    16621 ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag
    16681 ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag ctgagatatg
    16741 tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa
    16801 tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg agggtaaaaa
    16861 tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac gtttttgcat
    16921 cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct tcaacgtttc
    16981 agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt tacctaagtt
    17041 gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga ctcgttgttc
    17101 aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga aacattccgt
    17161 tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag ctaaatcaat
    17221 cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt gattctgaag
    17281 aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat tgaaatcaag
    17341 acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg cttggatctc
    17401 taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag ttgcaccgtc
    17461 taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc catgctcatg
    17521 catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg ctcgcaaaat
    17581 ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca tatgttctaa
    17641 cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa aaatggctat
    17701 cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt ggctgacact
    17761 tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa tgataacata
    17821 atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt cagcgtatag
    17881 ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct gctctgcgct
    17941 ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct gagaaacttg
    18001 taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca tagaccctgg
    18061 caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga tccgaggttc
    18121 ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg tcaaacttgt
    18181 ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat tccattttcc
    18241 gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt ggcaaacaaa
    18301 ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca tgagacatta
    18361 aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc gccttgacgt
    18421 tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc ttggccgata
    18481 cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg gaacatgata
    18541 tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac tccgtttgtc
    18601 aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa aaattcatct
    18661 tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg tttttcattt
    18721 tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt ccagcctgct
    18781 ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc agcagcatga
    18841 tgtgtagcgc gctgcggtag attgatgttt tgatctttat atttagaaat aacttcaaat
    18901 cctctttttc tcaccggttt tcctccctaa ataaactact cctattctaa catatccgag
    18961 gctgtaaaag actttcttta caaattcttc aagaaattct gctatcttaa agtaaaccat
    19021 taattcgttc tttttccaaa cgactgcaag ttgtacaaaa aaactatttt ttacatgact
    19081 ttactcgtca aaacaagtag aatagaacga aattaatgag tggatatttg attgaggtgt
    19141 cacatgcgta aagaaattaa aaatgcaaaa cgaattattg tcaaagtagg aacaagtaca
    19201 ttgatttatc ccaatggaaa tatcaatctt gcggcaatcg atcagttagc ttttgtcctt
    19261 tctgatctta gaaatcgggg aaaggaagtg atcttagtct catcaggagc tgtaggcgtt
    19321 ggtatgcata aattacattt gtcagaacgt ccgactttga ttcctgaaca gcaagctgcg
    19381 gcggctgttg gtcaagccga actgatgaat atctataatc aaagattcag tgtatataat
    19441 caacagacgg cacagatcct cgtgacaaga gacgtggtag attatcctga aagtcgtaaa
    19501 aatgtgagga atacattgga acagcttttg attatggaca cgatccctat cgtgaacgaa
    19561 aacgatactg tagcagtgga tgaattagat catctgacaa aatttggcga taatgatcag
    19621 ctgtcagcca tcatggcaaa acttgtcgcg gcagaccttt tgatcgtcct ttctgatatc
    19681 gacgggtttt attcagataa tccgactaca aatcccgagg ctcagatgta tcgctatatc
    19741 gataaaatcg atgaaaagat tctggcacaa gcaggtggtg ctggtagcac atttggaaca
    19801 ggcggtatgc agagtaaatt aaaagccgct tctaggatat ttgaagcaaa cagcgcaatg
    19861 attttagcaa atggaaaaaa tccggcaatt atttttgata ttcttgcagg aaaagaagta
    19921 ggtactttat ttaaggagga cttgaaatga ctgacttgat gcaattagga caacaggcaa
    19981 aagatagcgc gcaacaacta gcacttatgc ctacaaaccg caaaaatgag ctacttgttc
    20041 aaatggcaca aaccatcaaa aagaatcaac aagcgatcat tgaagcaaat cataaagact
    20101 tagaaaaagc agtcgaaaac aatatcagtg aaacgatgca agaccgcttg cagctgacac
    20161 ctgagcgaat caatgccatg gctacagaga tcgaaaaaat tgcttcgtta gacgatccta
    20221 ttggaaaagt agatgagatg tggacgaaca ccgacggatt gagaatcggc aaaaagcgcg
    20281 tgccactagg cgtgaccggc atcatttacg aatctcgtcc aaatgtcacg acagatgcag
    20341 ctagtctagc attcaaatca gggaatgccg ttatccttcg cggtggcaaa gaagcattct
    20401 tctcgaatca attattagtc caattattgc agcaagtact ggtcagtgct ggcgaatctc
    20461 cttatgccat ccagtttgtc gatgatactt ctcatgaaac tgcaggacag ttgatgcgat
    20521 tgacagaata cttagatgtg ctgattcctc gaggcggagc aaaattaatc caacgagtca
    20581 aagaacaggc aacggtacct atcattgaaa caggtacagg aaataatcac gtatatatcg
    20641 ataaagaagc acagttatct atggcagtga acatcatcgt caatgcaaaa gcttcccgtc
    20701 catctgtctg taacgcggca gaaacattgc tgatccattc tgagatcgca ccttttttct
    20761 tgccagctat tgaaaaagaa ctggtagaac acggggtctc tttacgtgcg gatgctcgtg
    20821 cgttagaata tctagagacg gctgctttag cagaagacac agattgggat actgaatatc
    20881 tagattatat ccttgctgtc aaagtggtcg attctttgga tgaagcaatt gcacatatca
    20941 atcggcataa tactaaacat tcagaaacga tcgtgacaga tagttatgcg gctagtcaac
    21001 gtttcttaaa tgaagtggac gcggcggctg tctatgtcaa tgcctccact cgttttacag
    21061 acggatcggt atttggtttt ggtgcagaga ttggtatctc tactcaaaag ttgcatgccc
    21121 gtggtccaat ggggttaaat gaattaactt ccacaaaata tatcatttat ggcgatggac
    21181 aaatccgcga ataatgtttc gtgtgaaaca ttattcccgc cttttgcctt t
//
LOCUS       DAXWLH010000040        21217 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXWLH010000040 DAXWLH010000000
VERSION     DAXWLH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.040"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(25..789)
                     /locus_tag="ACGE36_001074"
     CDS             complement(25..789)
                     /locus_tag="ACGE36_001074"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFF8383488.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(790..1737)
                     /gene="prmA"
                     /locus_tag="ACGE36_001075"
     CDS             complement(790..1737)
                     /gene="prmA"
                     /locus_tag="ACGE36_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFF8383489.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(1750..2238)
                     /locus_tag="ACGE36_001076"
     CDS             complement(1750..2238)
                     /locus_tag="ACGE36_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFF8383490.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(2204..2875)
                     /locus_tag="ACGE36_001077"
     CDS             complement(2204..2875)
                     /locus_tag="ACGE36_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFF8383491.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3013..4299
                     /locus_tag="ACGE36_001078"
     CDS             3013..4299
                     /locus_tag="ACGE36_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFF8383492.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            4371..4565
                     /gene="ssrS"
                     /locus_tag="ACGE36_001079"
     ncRNA           4371..4565
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGE36_001079"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4643..4927
                     /locus_tag="ACGE36_001080"
     CDS             4643..4927
                     /locus_tag="ACGE36_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383493.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            5000..5479
                     /locus_tag="ACGE36_001081"
     CDS             5000..5479
                     /locus_tag="ACGE36_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8383494.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            5702..5845
                     /locus_tag="ACGE36_001082"
     CDS             5702..5845
                     /locus_tag="ACGE36_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFF8383495.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            6190..6318
                     /locus_tag="ACGE36_001083"
     CDS             6190..6318
                     /locus_tag="ACGE36_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383496.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            6423..8006
                     /gene="abc-f"
                     /locus_tag="ACGE36_001084"
     CDS             6423..8006
                     /gene="abc-f"
                     /locus_tag="ACGE36_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFF8383497.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(8443..9627)
                     /locus_tag="ACGE36_001085"
     CDS             complement(8443..9627)
                     /locus_tag="ACGE36_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFF8383498.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(9652..10659)
                     /locus_tag="ACGE36_001086"
     CDS             complement(9652..10659)
                     /locus_tag="ACGE36_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8383499.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(10831..11190)
                     /gene="comGG"
                     /locus_tag="ACGE36_001087"
     CDS             complement(10831..11190)
                     /gene="comGG"
                     /locus_tag="ACGE36_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFF8383500.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(11162..11587)
                     /gene="comGF"
                     /locus_tag="ACGE36_001088"
     CDS             complement(11162..11587)
                     /gene="comGF"
                     /locus_tag="ACGE36_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFF8383501.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(11550..11897)
                     /locus_tag="ACGE36_001089"
     CDS             complement(11550..11897)
                     /locus_tag="ACGE36_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383502.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(11869..12339)
                     /gene="comGD"
                     /locus_tag="ACGE36_001090"
     CDS             complement(11869..12339)
                     /gene="comGD"
                     /locus_tag="ACGE36_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFF8383503.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(12314..12592)
                     /gene="comGC"
                     /locus_tag="ACGE36_001091"
     CDS             complement(12314..12592)
                     /gene="comGC"
                     /locus_tag="ACGE36_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFF8383504.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(12589..13638)
                     /gene="comGB"
                     /locus_tag="ACGE36_001092"
     CDS             complement(12589..13638)
                     /gene="comGB"
                     /locus_tag="ACGE36_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFF8383505.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(13595..14524)
                     /gene="comGA"
                     /locus_tag="ACGE36_001093"
     CDS             complement(13595..14524)
                     /gene="comGA"
                     /locus_tag="ACGE36_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFF8383506.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14656..16410
                     /gene="ade"
                     /locus_tag="ACGE36_001094"
     CDS             14656..16410
                     /gene="ade"
                     /locus_tag="ACGE36_001094"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFF8383507.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(16508..17170)
                     /gene="trmB"
                     /locus_tag="ACGE36_001095"
     CDS             complement(16508..17170)
                     /gene="trmB"
                     /locus_tag="ACGE36_001095"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFF8383508.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(17240..18022)
                     /locus_tag="ACGE36_001096"
     CDS             complement(17240..18022)
                     /locus_tag="ACGE36_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFF8383509.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(18226..19437)
                     /locus_tag="ACGE36_001097"
     CDS             complement(18226..19437)
                     /locus_tag="ACGE36_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383510.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(19430..20167)
                     /locus_tag="ACGE36_001098"
     CDS             complement(19430..20167)
                     /locus_tag="ACGE36_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383511.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            20305..20739
                     /locus_tag="ACGE36_001099"
     CDS             20305..20739
                     /locus_tag="ACGE36_001099"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8383512.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            20732..21076
                     /locus_tag="ACGE36_001100"
     CDS             20732..21076
                     /locus_tag="ACGE36_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8383513.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         6468 a         4443 c         3789 g         6517 t
ORIGIN      
        1 atactctttt attaatgaag aacgttagct gaaagaagga ttcattaatt cccattgata
       61 gctagcagca cttaataaat aaagaggtgc tgtttctgta cgaaggattc ttggtcctaa
      121 tccacaaagt atccctttgt gttctacaaa ataatcgact tcatcgcggg ataatccacc
      181 ttctggtcca aagacagcta agattcgact atctggtttt gtttcactaa gaattttggc
      241 tgcatttgat ttttcgcctt gctttgctga ttcctcataa gcaaccaaga ccacgtcata
      301 atcatctagc cgctcaaata aatcttgttt cgtttccaat aactctactg atggcacgat
      361 ctgccgatgg gattgttccg ctgcctcttg agcaatcttt tcaagccgtt gttgtcgttt
      421 catacgtttt tttgcatccc atttaacgac agaggctgat gccgggaaac caagaaaagc
      481 ttgcgctcct aattcagtgg atttctgaac gatccaatcc agcttttccc ctttaggata
      541 gccactcgct atggtaatcg aacttggcag ttccttttga ttttcttctt ttttaatttc
      601 tttcagataa acagcctctt cagtaatctc cgtgatttct gcaacaatgg ctatttgatc
      661 attgaatgcc aaaaaaactt catcctcggg ttccatcctc ataacccgta caatgtggtg
      721 atagttttcc ccttctaaaa caaaatagtc ttttgattca tatgattctt ttaaaaaata
      781 ccgctgcatc tattccacct ctggtttttt caaaataatt gcataccagt ctttttgctg
      841 gaaaatttga tccacgataa acccttgttc taacatggca tccagaacca tttgcttctt
      901 ctcttcaatg attcctgaaa cgatcagcgt gcctgtttgc ttcagtaaac gccaagcatc
      961 tgggatcatc aataagatga tatctgctaa aatgttcgca acgattacat cactttcaat
     1021 ctcgattcct gtcaaaagat cgttagctga tacatggaca tctttggcta tttcattcat
     1081 atccatattt tctttcgccg aacgaacggc tacttcgtct aaatcaaagg catagacatc
     1141 ttttgcacca agatattttg ctgcaatact tagcacgccc gacccagttc ccacatctag
     1201 caatgtttca ccaccacgca agactgtttc tagtgctgtc aatgtcaagg aagtcgtggg
     1261 atgagtgcct gtgccaaatg ccataccagg atctagtgtg ataatttttt ctagaggatc
     1321 ttctgtctgg tagttttccc agcttggcgc aaccgtcagc attcgcgtaa tacgcaccgg
     1381 atgatagtat ttcttccagg ctgttgccca gttgctttcc tctacttcac taactgttac
     1441 tttatttttt ccaatagcta gaccaaactc aggtaattgt gtgatacgat ctttgataaa
     1501 aggcaagatt tccggtaaga aagtcgtttc cggaaaataa gccatgacta gtgcgccttc
     1561 tttcagcgat gtaaaatcat ttttatctaa aatctcacca tattgatccg actgaaaatg
     1621 ttcaacatct aaagaatctt caatcgcgac accactcgcc cctgcctcca tcaaaatatt
     1681 agcaactgct tcgactgctt cactttcagt ttctactttt acttcattcc atttcatatc
     1741 tgtcactcct taataacttg ggtacggtaa ataagtatct gcttcatttt gctgttttac
     1801 ttctactaat tctgctagtt gctgattatc ccagtgaagc tcgaatacag cttcgtcatc
     1861 gtcttcatcc aaaaaatcaa gcaaatcgct ttgcccttga gctaatactt catttagata
     1921 ttccactaaa gaatcagcta ctgattttgc caaccctttt tttccttcat aagggattac
     1981 tgctagataa ttgcgttcat caaaaacaga tttctgagga ttaaaaagaa gtacgccgtc
     2041 ttcaaattcg ataattggtt cttgcgaaac gatcccttct gcatcatcaa tctcaaattg
     2101 tgctttattt tctgcaaaca agcgaacaac gatctcgatc gtatgatttt ttgtatccca
     2161 atctaaagct acatcatagt ctgtcacttg tttttcaatt atttcatcta aatatgttaa
     2221 tagatccttc tttttcattt tctttcctcc aaccccaatc tttctcgatt tggttttttc
     2281 tttgtaaaga gatataagga tttcccgaca caacaaaacg caacggcttt tctgtccata
     2341 cacctttgtt tgggataccg attctaggca aagcattgat cttttttggt gtcttttttt
     2401 tctcaaaaac taagcgtaac gaactatctg ctatagactg accataaagt tcttgaggaa
     2461 ttgctaatgc ttccactaat tttcccggac cgttgctgat atctggacca gtttttccgc
     2521 cacgattttt actcatttga tcaatcattg cggcgggttc gattgcacgt atcattactc
     2581 cttgaggaat cccttctggc tgagtaatga tgttcaaaat ccgatgagta tgcatcgtgt
     2641 aaaggtagat cgtacctggt ttttcataca ttgcccgcac acgcggtgtt cttcttaaac
     2701 catagctatg ggcggcctcg tcttctggac ctaggtatgc ttccgcgtct acaatatatc
     2761 cagctaatct tccttctggt gtgtcatgct ccaaatacat ccccaataaa tcccgtgcca
     2821 cttctggcgt cgttttattc caaaaaaatt catctatctt ttgtttatcc gtcatttttc
     2881 tcacctactt tatcatacac aatattagtt ggataagcta tgaaaacaaa acaacagaag
     2941 gctgttcttt ctaggcatct tgctaaatat gatataatta agggacgtaa aaacaagaag
     3001 gaggaaaatc ctatgcaaca gccattggct tatcgcatga gacctcgaac gatcgatgaa
     3061 gtcgttggtc agcagcatct agtcggggaa ggaaaaatca tacgccgcat ggtcgatgct
     3121 cgcatgctct cttccatgat tctatatgga cctcctggta ctggaaaaac gagtatcgca
     3181 agtgcgatcg ccggctctac taattatgca tttcgcatgc taaacgccgc tacagattca
     3241 aaaaaggatc tacaagttgt agcggaagaa gcaaaaatga gtggtacagt gatcctctta
     3301 ttggatgaag tacaccgatt agataaaaca aaacaggatt ttctgcttcc tcatttagaa
     3361 agtggccgaa tcatcttgat cggagcgact actgagaatc cttatattac catcaatcca
     3421 gcgatccgca gccgtacaca gatctttgaa gtcaaaccat taacagaaca agacatccag
     3481 ttggcagtcg aacatgcttt aagggataaa gaacgcggcc ttggacaaca agcaatccag
     3541 ttagacgaag atgctcttct tcacctttcg cgtgcaacga acggcgattt gcggagtgca
     3601 ttgaatgggc tggaactggc tacactatcg actccttcag ataaagaagg aaggattcat
     3661 cttacactgt cgatcattga agaatgtgtc caaagaaaag ctcttaccca tgacaaaaat
     3721 ggagatgctc attatgacgt tatttcggct tttcaaaaat cgattcgtgg aagtgatgtg
     3781 gatgctgcac ttcattatct cgcccgttta gttgaggcag gtgatctggc aagtatttgc
     3841 cggcgtctga tggttatcgg ttatgaagat ataggtttag gtaatcctgc tgctgcggct
     3901 cgtacagtga acgctgtatt agctgctgaa aaattgggat tgcctgaggc acgtatccca
     3961 ctagcagacg tcgtcgtcga tctttgcttg tcgccaaaat ccaattctgc ctacatggca
     4021 ttagatgcag cacttgctga tattcgagag ggcaaagctg gagatgttcc tgatcatttg
     4081 agagatagcc attacaaagg tgcgaaatca ctcaaccgtg gcgtgggtta tcagtatcct
     4141 catcattttg atcaggcttg ggtcaatcag caatatttac cagataaact gaagaatgct
     4201 cagtattatc agccaaaaga tacagggaaa tatgaacagg ctctgggtca acagtattac
     4261 cgtataaaag agtggaaaaa gcatcctcca aaaaactaga caagaaaacg gttctgtttc
     4321 tgcgagctct ctcattgctc tttttaaatt catatgctac tatagaggtg ggaaatctgt
     4381 gatgttcgaa ttgtcctctt tgtgtgttga ccgaacattt ttattgatat cttgggaccc
     4441 gtcttgtgtc actgctggta acatgcctta tcatttggta gttcgaagct acgaccatgg
     4501 gacccacctg cttgaattcg cgggatcaat actacaggac aaacaacggc attaacggag
     4561 attaccatct tttaaagaat aaagcaattt gctcttggca aattgctttt tctatttgtt
     4621 gcttattttg gaggatttgt tcatgattag gattttaatg attatcgcca ctttagtatt
     4681 attattcgtt agctattatt tatttaataa acaggatatc ttttttgtat tgatcaacaa
     4741 aaatgataaa aatcaaggat tcctacaatt ttacggagct gcatatgctg ttttaggcgt
     4801 aatgggtatt ttaacagctt ttttcaatca acgctttatt gccttggtct ttttactgat
     4861 cgtaattatt gtttctgcca ccttcagtat ccgatttgca aaaaaaattg ctgaaccaaa
     4921 gcagtaaata attctctttt tccctaaagt gttctacaat aaaagagtac aatacgattg
     4981 acttgaggag tgaatgctta tgttacaaca atacaaaaaa atcatggttg ccgtagatgg
     5041 ttctgatgaa gctgaattag cttttaaaaa ggcggtcaat gtagctatcc gaaataacgg
     5101 agaacttcta ttagcacatg tcattgacac acgttctttc caaaccgttt catcctttga
     5161 tggaatgctt gcagaacaag ctactgaaat ggcaaagcaa acattggcag attatgaaag
     5221 caatgcgaag aaagctggct tgaataatgt aacctctgtc gtagaatatg gctcaccaaa
     5281 acaaattatt gccagagaaa tcccagaaga taatcaagta gatttgatta tgttaggagc
     5341 cacaggattg aatgctgtag aacgtttatt tattggttct gtttctgaat atgttattag
     5401 aaacgcagct tgcgatgtgc ttgttgtccg aactgattta gaaaaccaat tgccagctaa
     5461 agaccacacc aaagcttaac acgtagacga cctgccaact atcggcaggt ttttttgcac
     5521 aaaaaaccgt gataaaaaca aaagacgcgg caagcactca tcggtcttta taatgtttaa
     5581 aataaaggga aaacagtcaa tgagttcaag taaaataaaa aaaagtgaac accagggcga
     5641 ttagtcgccc tggtgtggtt taaaacgacc taagcatgcc aaagcttatt ggttcgtcaa
     5701 tgtggaagtt tccgatgaat ctgatacgaa aaaatatgaa gtatcgatgg cggataccga
     5761 ttttatcgcc aaactaaaat tttatttcgg gttcgggtac cgcgagaagg caagtgctac
     5821 attcggtaaa tcattcaaga attgatgaaa ggctaccgct tgccattgca accacaacga
     5881 tactcctgaa cgcgatgttg gtgagatttc caaagagaag agctgtttaa tgcaacggta
     5941 ttaagcaaat ggcaaaagga agtcatgtag acaaagtttt ttatcaaaca ccatatgatt
     6001 tcgcctgaac gctggtttag ctcagaatac aatgatgtgg tccacgagac attaccctca
     6061 gtcagataaa aaaagctctc tgacagaatt acacatcgat aaatattaag taaatgtcag
     6121 cgctagttga actttctgac actttcaggt acactggaaa agacaactac acgaaggagg
     6181 gaaaatacta tgatgcgcat gactaaaata acaagtaatg aaaattacca acgataccat
     6241 atcgcattta gccatgcaat tggttttaca gttgctgttg acgggacaag tttgtccaaa
     6301 acagttgaat tcacgtaaaa tcaatggcca tctcaaggta tttttctcat gataatctga
     6361 gtttaaataa cacaggccat ggataaccat ggcctttttg agtggtctca aggagagaga
     6421 tcatgatcat acaagcaatt tctattaaaa aaacaatcgg cgggaatcca ttatttgagg
     6481 atttatcctg tcaaattgaa gctggtgaaa aaattgcctt agtcggtatc aacggaactg
     6541 gcaaatcaac ctttttacaa atcttgaccg gcagagaagg tgtcgacagc ggtgttatca
     6601 gtcgcaaaaa aggcttaaag atcgggacgg tcgaacaaga acttacagtt tccgaagcga
     6661 ccgtcaacca ctacttgctc cattctgccg ctgagataca ggacctcaaa cagcaaatga
     6721 gtcgctacga agcattgatg acagaaccag atacaaactt agagaagtgt ctggcccgtt
     6781 acggtgagct gcagcatcgc tttgaagaac taggcggtta cgtattagaa gaccggattg
     6841 cagcgatctt gcaaggctta gggattccac accgtcagga agcacggtta acagaactta
     6901 gcggaggcga acgagttaaa gtcgctttag ccaaaatttt agcagccgat gccgatttac
     6961 ttttactcga tgaaccgacc aatcatttag atcttaacag tattcgctgg ttggaaaact
     7021 atttacagaa tacaaaaaaa tcggttctgg tcgtttctca tgatcggcaa tttctcgatc
     7081 aagtcaccac aaaaacatgg gaactggaag aaggtgcatt gattgagtat cccggaaact
     7141 acagccgttt tcgagtcttg aaagaagctc gcttggctga gctgacaaaa aattacgaac
     7201 tgcagcaaaa agaagtccag cggttgaaag tcatgattcg tcgttttcgt cagtgggcac
     7261 atgaaggaga caatgagtcc ttcttcaaaa aagccaaaga actggaacgc cgcctggcga
     7321 aactgacact cgtcaaaccg ccacctcccc cgaaaaatcg gctgcaaagc ttgtctaatg
     7381 gtggaaaatc tggcaaagaa gttttcatca ttcaaaactt gcaccagcag tacgctgatc
     7441 aagtactatt caaagacagc agttttgcag tctatcgtgg agatcattta gccatcatcg
     7501 gcgacaatgg tgcaggaaaa tcaaccttac tcaaattatt actcggtgag gaaaagcctt
     7561 acggtggcac catcaaatta ggtagtagct tgcaaatcgg ttatttgccg cagcagctgc
     7621 agtttgccga tcccgatagc cgtttgctgg cttacgccat cacattgact ggaaacgaag
     7681 aagtcgctcg acggcaatta gccaaaagtg gcttttatca aagcgatgtg ggtaaacgga
     7741 tcaaagattt atccggtggt gaaaaaatcc gtttggcgtt gctgaaactc tttttagaaa
     7801 aaatcaacgt cctgattttg gatgaaccaa ccaatcacct cgacagctat gctcgagaag
     7861 aaattgaaga aatgctgcaa gactatcaag gaacgctcct agcagtcagc catgaccgct
     7921 actttttgca gcaacacttc caagaagcac tcgttattga tcaggagcag atcagccgcc
     7981 agccgctcgg ccaactgatt gactaggacg ttttttcgca acaaaaaact tgattttcat
     8041 acctgtgctt tcacgattca ggcaaagtac aggtggaaaa tcaagttttt attattcgat
     8101 caatgaatgt ttcggatcgg ttttctagct aaacataaag cgaagaataa agtcgacagc
     8161 ccacgaggct gatctaaaaa tgttttttct ttttacatat gtaatgtccc ttataaagat
     8221 gaatgaaaat aaaatattgg gattttcctg aaaatcaata aataaaatgc cagtcttttc
     8281 ccttttgacc acttttccaa gcaaaattcc ctaagaagat cctgtaaaaa agaagggaaa
     8341 ttgaagttaa attaccattt tgcggtcttc tccgcaaaaa aaacacatgc aagaattccc
     8401 tcttgcatgc gtttcatctt ttatcctaac gaatgctttg gcttagccgc gcagacgctc
     8461 cacatcacgg gcaatcatta gttcctcatc tgttgggatc actaatactc ttacttttga
     8521 ttcttttgta gagatatcta cttcttcacc acgcaattca ttcaacgctg gatcgatttc
     8581 acaaccaaac caagacatac ctttgatgat ttcactacgt acatgtccgt cattctcgcc
     8641 gataccagca gtaaagacaa ttgcatcaac accattcatc gttgtcacat aaccgccgat
     8701 gtattttcgg atgcggtcca cgaaaatgtc ataagctaaa cgaatatgtt ctttttcata
     8761 gttcttttca agatcacgca tatcactaga caatcctgtt aggcctagca gaccagattt
     8821 cttgttcaaa acattgatca tttcatcaat atcaatctct aatttttcca ttaaatatgg
     8881 gagaacagct ggatcgatat cacctgaacg tgtacccatt gtcaccccag ctaatggtgt
     8941 gaatcccata gaagtatcca tggatttacc tccatcgact gctgtgatcg atacaccatt
     9001 tcctaagtgg caagtgatga tttttagttc ttcgatcggt ttgttcaata attttgccgc
     9061 acgttccgac acataacgat gacttgtgcc atggaagcca tattttctta ctttgaaatc
     9121 ttcataataa gacaccggta agctatataa ataattttgc tttggcattg ttgagtggaa
     9181 agatgtatcg aatacagcca cactcaaaat gtcaggtaaa atctttttaa acgctttgat
     9241 ccccattaag tttgcaggat tatgcaaagg agcaaattct gctaattcag caatgcggtc
     9301 cattactttc tcatcgatca ctactgaatc cccataagtt tctcctccgt gaacaacacg
     9361 gtgccctact ccggtgattt catcataaga gcttaaaatt tgcagttcaa tcaactgatc
     9421 taacagcatt tttactgcta cttcgtggtt ttcgatatcc atgatttgtt cgaatttttg
     9481 attatcgccg tacttgatgg taaaaataga atcttgtaaa ccgatacgtt cgacgatccc
     9541 tttcgcaact acggtttctt ctggcatttg atataattgc catttcaaac ttgaactccc
     9601 agcattgatt gcaattgttt tagacatatg actctccttc tcgttgaatg attacagagc
     9661 agatgatttc cagtttttaa actcgttgaa aaattccgtc accttctctg gttcttttaa
     9721 tgaagcaagt ttcacaagca agacttcttt tacttgttgt gcttgtggtc cttttttctg
     9781 taagatcaaa atactttttt gcgattgttt attacggaac aattcatctg gcaattgaat
     9841 cattccttga agataacctt cttctttaaa ccattttttg atttcttcac tttgatccgt
     9901 ttctaaaaat ccactcggca tcaaaaacaa accaaaacca tccggcttca catatttcat
     9961 tgattgctct agcaataaat gatgtgcata actatggcct tcttcggtac ttgttaaaaa
    10021 ctcttttgct ttttcgtcat tcggataata accgattggc aaatcgctga ttgcaaaatc
    10081 gacaggatct attaataatt cctgcaaccc gtcttgatga aagtattgaa catttgcttg
    10141 tgtcaaatca cttgtagaag ctgcgacagc caacaatgta tcatcaatat ccacaccaat
    10201 tccttctgtt tggtaccccg cattcgatag gtttaaaaga actgttaata acaagttgcc
    10261 cattcctgcc gcaatatcca gaatcttaac cggtgctttt ttatttgtgt atagttgttc
    10321 aataagaaaa acaaataaaa aaccgatact atcaggagtg agttgatggt tcgcctgcaa
    10381 atgttctgtc tggctgccct tcaataaaag taattgagac agtctgcgcc attcttctgg
    10441 ctcgaaagaa agtttcttca actcttcata aagagctgtg atccgttgcg ttgtctcttt
    10501 tgtcggcact ccatccacca cacgtacttg ataatcatct attaaatttt ctgcattttc
    10561 gacataggct tctaaaaagg aagttcccaa tgcattctgt aaaagctgaa tagcttccag
    10621 gttttgatta aacccttgtt ccattttttc tggtaacatg gtgcacctca tttctagata
    10681 tattataaca attttttcac ctgtttaatt ctatcgaaca attgacaaaa tttcaatcgt
    10741 tttcactgtt ttttatttgt gaaacggcaa acattttttt attaaaaaca gtgttataac
    10801 agaaaagagg gcgttttcac gaacattcca tcattctgta ctagtatctg tatcattttt
    10861 tacttcttct gtgaaccgaa agataaacgg tgagacttcc acctctaaga atagggactg
    10921 ttgtacccac tgatatgtta cagatccagt tgtgtagtaa aaaactcctt ccgaactttc
    10981 ggcaagcgta gactgttccg ataaagccat ctgcttcatt atttttgcta tataataatt
    11041 tttgattcgt atattaagat ccattgacaa acggtagctt tctaatgctg ccatcaataa
    11101 aaacgtacat aaaagaaata aaagcaagac agaaaataaa atagagccct tataacattt
    11161 tctactgatc gttttttgtc catatggcat atccatcttc tccattttca aagtgtacat
    11221 aaaaagaaat tgtgtgttct ttttccaata attgaagctc agtgatactt gttaacatag
    11281 gttgatgtcc gccggaagtt cttcttttac gtatcattgc ctgatatctt tcgatgatat
    11341 actgtttatc ctcgatcgtg tagtgcagtt gctgattttt cacagagaca ttgtgactgc
    11401 tcttgagttc ttcttctaat tgtgctaaaa atattagcca ctctttttga tctttacgct
    11461 gcaaaaattc acttacttgg atggtttgct gaatcaaaag attgaatgtg agcaatagac
    11521 tgctcaatat catcagtcca attagacatt caatcagcgt aaatgctgga attacttgtt
    11581 ttttcaatac tgatcgcttc tttctccata ttgacaaaca ctttttcgat tactccatta
    11641 ttatcgacaa tttggatatc gtatgggatc atttcttccc ttcgctctgg tcttttgtga
    11701 tacatccgct gcgtcttgat atcttcataa atcattcgaa taaatagcag ttcctcccca
    11761 cttttctttt ctgcctgaag caaaaacagc tggccaaagc tcactaagta gacacagaca
    11821 gaagcgatca taagcgctgt aagggactcc aacaaaacaa atcctgtttt attcaattct
    11881 ttttgtatag cgaccactcc ctatctggaa ttgatacgta atcgtccgct ctttttcttc
    11941 ccaataaaat tgataagctt ttagtgaact ttcattcccg gttctagcag caaaagtaat
    12001 agaagcatgg cgtttcaagg taatctcatc aggaatatct aatacttccc aatttatttt
    12061 atctggattc ggaacatgaa aaatgatttg attctcttca ctattccaat aaaagcctgt
    12121 ttggatctga ttcacaatag cgattttttg cgtagaatag attcgttttt caaattggcc
    12181 gaaaaattga tagactgcca attcattctt ccatgcagaa aatgcgagaa ctggaaaaga
    12241 aaggatcgtt gtaagtaata aaatcagtaa gagtgattca aacaacgtat atcctgatgc
    12301 taaaacctta ccttcaaggc cgctgataga tttctgcatg ttctcgtttg atatagcctt
    12361 cattcaacaa ttcctccaca gtaggaactc ttccattatt ttgaagcgca tagagctctt
    12421 tttgtgtgtc taccaactgg atgatcgctt cacgactttc ttggtctaca tgattcctat
    12481 aacgcgacaa attagggacg aacaacaaca ctaatacact gatgatcagt aagacgatta
    12541 gcatttcgat caatgtaaaa ccgctataat aagatatttt tctctttttc atggcaaaac
    12601 ctcctctatg cttccgtaaa caggtaataa aacagcggca taaactaata aaatcaaagc
    12661 cgctacgccc aaaaaaatga ttggctgaat aaaatgcatc cagtgatgaa gacgtttgaa
    12721 aagctgtttt cttgtccatt cgctgtaaaa aatcagttct ttacctaaac ttccctttgc
    12781 ctctccctgt aaaacaattt tactgaattc ttctgataga aaacgataat tctggaactg
    12841 ctcagccaat gacatacctg cctccaatcc ttgaatcaac ttagaggcaa gatgctgaat
    12901 cagactttct tgtttcgttt cctttaagca gactatgatt tgccttaatt cgaaaccttc
    12961 atgaaaaagc tttccaatct ctagtgacaa ataagccgat tgatacaagg aatatatcgg
    13021 tccaatcaat aattgtctgc ttatccattc actctgatca acaggagaca acttcatcag
    13081 acgatacctt actagtaaag ccaatagagt aaccgctagg atgaatagta gaacatacca
    13141 atgaaagact tgtatcagct gaatacccca atgctcttgc atgaccattc ctgaaccaat
    13201 aagctgaggc aaaacgaaca accgcatact aaacaagata cctagcaaaa aggtgagcaa
    13261 tagcatcgga tatgcaagca gctttttcaa atcattgcga aatccttcaa caagccgaaa
    13321 ctgtttggca atttccaaca aagttgtcgg cagattccca tgacgttcag ctaactcgat
    13381 ctgtaccagt tgctctgctc catatcctat ttgcgaaagt acctcactaa acgcttgccc
    13441 ttcctttagc tgatgttgga tatttgccaa aataacaggt ggaaacgaac ggatcgtgag
    13501 taataaataa agggattctt gcagactaaa accagataaa agcagttctc ccatggtttg
    13561 tgcaaactgg ttttgctggc ttcttgttaa tcctttagaa tagttctgac tgccaaatat
    13621 tttcctgagc ttcttcataa ctttcctccc atgatttttt tactgattct tcctccataa
    13681 attgataact ccataaaaca gaaggatggt gattggcatc ttccaaaagt tcttgataaa
    13741 tgacaccagc taaacattca gatagctctt cttttttctt tgtcagttca tggattctga
    13801 ccaatgtacc ttcaagattg cgtgcatgta cggttgcaaa aacccggtgc cctgttagtg
    13861 ctgctcttac cgcagcatgt gcagttgatt catctcttat ctcgccaatg attagcaagt
    13921 ctggacgatg ccgtaatgcg agtctcaaaa gtgtatcata agtctgtgct attttcggat
    13981 tgatctgtag ctgcagaaaa aacggttctt ctatctctac aggatcttcg atagtaatca
    14041 cttgcaattt ttcttccaaa gccaaccgat acattagaga agtcttgcct gatccgactg
    14101 gtccgctgaa aaggtataat ccacgtctga cggttttctg taaaatagtt gcaaaatctc
    14161 gagaactaaa gcaacgatgg ggtgtttcaa aagtaccgta taatagccga atcactaaac
    14221 tctctcgttg aaggtaatct cctacagaag acaaccgcag acgaacttcc tgtttttcta
    14281 aagaataagt aaatgcacct aactgagctt ttcttttttc tccgatatcc atctgtccca
    14341 aatacttaaa tcttgagatc agttgctgtc catctgccga agagactttc ttccagaatg
    14401 ttcgtttttt tccatagcga aaatagatgt aaaatacttc tgaagctggc aatatatata
    14461 cgtcttgggc atgtttggca aaactttcac ttatcatttg atccgataat cgttttattt
    14521 ccattattct cccctccaca aaaaacatac gtctttttga tctttttgtt tcaaataaaa
    14581 tgaatttaca aaaaaatatt ggcaaaaacc gaatttatgt tatacttata aaaatttatc
    14641 ttgggaggca ctccattgga aacaacatta aggaaatata tcgaagtagc aagtaaaaaa
    14701 aagcctgctg acctagtgat caagcatgct aagatcgtca acgtctttac gaaagaaatc
    14761 atggaaaaag atgtagcaat ctgcgaagga atgatcgtag gaatcggtga atacgaagga
    14821 aaaagcgtac ataatgctaa tgggcaatat ctagtgccag gattcatcga tggccatgtg
    14881 catatcgaaa gctcactttt atcaccaagc gaattttcaa aagtatcctt gcttcatgga
    14941 gttactactg tagtgacaga tccccatgaa ataggaaaca ttgctgggag tacaggtctt
    15001 gagtttatga tagaagatgc aagacaaaca ctcatgaata tcttttttat gctaccttct
    15061 tgtgtacctg taaccccttt tgagaccaat ggtgcaaatt taaatgcaaa aaaattagcc
    15121 ccttttttac aaaaacccga agttttaggc ttagctgaag tcatgaacta tcaagcagta
    15181 gcaaataatg aaacagacat cctagaaaag atccaattga tgcaccagca taagaaaaaa
    15241 attgacgggc atgcagctgg tatcggcatg gaagagttaa atgtttatcc tgcagcaggt
    15301 attcggacgg atcatgaagc tacaactgca aaagaagcta aagaacgttt ggatcttggt
    15361 atgtatttaa tggttcgaga agggaccgtt gcaaaagatc tggcatcgct actaccggcg
    15421 atcacacctg aaaattcaag aagatgcttc tttgtcaccg atgataaact gatcaatgat
    15481 ttagtaaaag aaggatcaat cgatcatatc gtccgtcaag ctattgcact tggaatcgat
    15541 ccattacaag ccattcagat ggcaacattg aatgcagcag aatgttttga tttgaaaaca
    15601 ctaggtgcga ttgcccctgg gtatcaagct gatttcttct taacagacga tcttaccaag
    15661 ctgccaattc atgaagtttt ctataaggga acattagttg ttcaatctgg gcaactccaa
    15721 gaccatttat ttgctgatca ttcaaattca tatacgtgtt gtctgccaaa actaaacgct
    15781 caaccattaa atccacattc gttagagctg cttctttctt cttcacaagc ccatgtgatc
    15841 gaaattatcc cgaatagtct agtgactaaa gatttggtag aaaaagtgga ccgacaaggc
    15901 gaacgattca ttccttcaat cgaaaaagac caattaaaga ttgcggtgat cgagcgtcac
    15961 catcaaacgg gaaacattgg attaggcata gtgaaaggat ttcagctgaa acaaggagcg
    16021 atagcgacaa cgattgccca tgactcgcat aatctagttg tcgttgggac aaacgacgag
    16081 gatatgctgt atgctgctaa tcttttgatt caaaaaggtg gcgggatgac cgttgtcaac
    16141 aaacagcagg aaatagcttg tgtttctctt cctataggcg gaatcatgac acaggaacct
    16201 ttttcgatcg tctatgaaca gttgaatgaa ttaataacta aagcacatca actaggagca
    16261 gctaaaacct ttgatccttt tttgacactt tctttcttaa cattatctgt catacccgaa
    16321 ttgaagatca ccgacaaagg attattttca ttttctcgct tccaattgat tccaccttgt
    16381 ggaaaaaccg atgaaataaa tacattttaa atcaaataaa tcccagaaaa tacattacat
    16441 ctgtaatgtt cattttctgg gatttattta tggatattaa tgtttttatg aaagcagaaa
    16501 gttagtttca cagacttttc tttttggtct tatcttgaaa ttgagcttct actcgataaa
    16561 tacgttgacc ttttgaagaa aatttttctt catactccgt cataatattg ccttcaaatt
    16621 gactttgatg taaatctaac catacctgtt tgaggatcat gccatattga gaaaaacttg
    16681 ccagagaata ctcaaacaat ccttgattat ctgttttgaa atggatttct ccatttggac
    16741 gaagaatctg ttcatctaca gcaagaaaag tcttatatgt caatcgccgt ttttcatgac
    16801 gtttttttgg ccatggatcg gaaaaattca gatagatttg atctacttcg ctgtcagcga
    16861 aatactctgt caacgctgat ccatccacat gtagaagttt caggttagga agcggttgtt
    16921 cgatcaactt gtctagtgca atcgaaacaa cactgacctg catttctact ccaatataat
    16981 tgatttcagg atgggctttt gccattccag tgatgaactg gcctttcccc ataccaatct
    17041 caatgtggat tggatgatcg ttttcaaaca attcattcca ttttcccttc cacaatgttg
    17101 gatcactaat tacaaaattc gggtgagccg ctaacatttc cgctgctccc ggacgatttc
    17161 ggacacgcat tatttatctc cattcctttc ctctaattaa aaaggtctgg ccaaatctcc
    17221 gcagagtttg ggccaaacct cagccactga aaattcgttt taattgtaaa atttcttcgt
    17281 tcatccgtcg atgatcttca cgtttgcaat atctggtgat ttcttggagc aaattcatgg
    17341 ccgcatacca atgaaatttt tcaagcattt cgtcagtcgg gcgtatgcca taactgatca
    17401 gccactgatt ccagctagac agtggcacat aatttcctaa aatcgttgca atatccaccg
    17461 cagggtctgc aaacatgatt gaatcccaat cgactagata taaataattt ttacatacta
    17521 accaattacg atggtttaca tctccgtgta ctactgataa gttttcctta tctattgacg
    17581 ggatctgctc acgaagaaaa cgatggacaa tttgcagaaa ttgatttttc tggagttctt
    17641 ccggtaattc tttttcatat ttttgcagca tcttctccgg cgtcatgaca cgaccaccga
    17701 tacgggtcag catggtttta agagaatttg aatgatgtaa atggtacaaa acatcaatca
    17761 cgtcgtttct ttgaccaatc tcattaggag tcagcaagcg accttccagc cactcttgtg
    17821 ctgtcaaagt atcgccattc cccgttcgtt ttgtccaaac aagttttggt gtaattcctt
    17881 cccttgaaag agcagccaac atcggagtcg tgtttcgttt aataaagact ttgtcctcat
    17941 cacgcatgcc tatgtatgtt tttcctgtgt cgcctttgat gggacgtaac cgccactctt
    18001 ggtctaactg aaactccatc atgcattctc ccgtcctctt actctttgtt ttgcagtact
    18061 tattattcta gtcgcctgaa cactacacgt caagcacata tacgttcttt ttcatttaat
    18121 tgccattcaa aaaaccagct tttctcaaaa aaaatagaac gaacagcatt cttactgtca
    18181 acgctctatt tttcattctc atttttaccc tacacagctt tgttttcagt ctgacatctt
    18241 cttcaaacga gttggtacat atagtttaac aaagagcata gtaacaatca aataagccgc
    18301 tagcggaacc catcgctgct gcccatctaa tacgactgca gcaatcaaac caaaaataac
    18361 cgacgcgata attaataccc atgtgagtag atactgaatc gcctgcttct tctgattctt
    18421 atggattgga taaagctgaa cgagaaccat atagcggaac tggttcgcta aaggtaacag
    18481 ttggaaaccg atcaaatata aaaataagaa accgataatc agactaaaat aaaagtcgac
    18541 tactccggca atcaataacc cacctataat tactaatcgg aaataaagac cactaaaatc
    18601 agaacctctt gccaatcttc ttgcaaacaa gtaaagataa gtgttttttg attctctttt
    18661 aatatgagtc aatataggat ccaaatagcg tcttcttttg atctttgccg taatttcagg
    18721 aacatctgtg aataaattga taaattgata gatctgatgc aacctatgtt cttctacatg
    18781 caccattttc tcccaatcca atcctttttc ttgttgcttc cacaaaaacc agtaatatag
    18841 acttgcctgt actaatgcac ctgctattcc tgcccatatt agccaataaa atgatagaaa
    18901 caaaaagatc agagaagaaa aaaaccagcc taatttccaa taacgaataa cttgttgatc
    18961 tagtcgaaac aattctgctc tcttgatttg cagatgactg aatttcaaac tccataatat
    19021 gatcagatag acgaaaaaca tcgaaaagga ttgctccgta ctaaccacca ctaatggcat
    19081 ggccaatccg catgccaata tcaaaaagat caacggaaaa atgcaagaat agcgaaaagc
    19141 tcgttccaaa taggtttgca tttctttttc tttaggaagt aaaaaaacaa tatctgctgc
    19201 ctctgcaaaa gtagccatct tacccacgaa taaacagagc atccaaaata cagagattat
    19261 gagccctcca attgcaaacg gtggctgcag ggttttcaac caattagaat aatataatcc
    19321 cacaccgcca atcaagaaaa gacaaactaa aacaaaatga tcgttcaata cataacgcat
    19381 atatttcatc attttctttt gatgtcttgc taaacgtttt tgaaaaaaac tactcatgtg
    19441 cctgctcctt tgttaaagca atatagattt cgtccaaaga agaatccggc agctgaaatt
    19501 cttttctcaa catctcaaga gaaccgtctg ctcgtatctc tccgttatgt aagaccacaa
    19561 aacgatcaca atatttttct gcagtagcta aaatatgcgt agacatcaaa ataccggctc
    19621 cttctttttt catttctacc atcaattcaa gcaatgcatt gatagctagt ggatccaagc
    19681 ctaaaaatgg ttcgtcgatg atatataaag aaggctcgat caaaaaggcg cataagatca
    19741 tcactttctg tttcattccc tttgaaaaat tagctgggaa ccagtcaaga cgattagata
    19801 accgaaaagt ctccaacaac ttttctgctc ttttcattgc tatatccata ggtatatcgt
    19861 aggccatcgc agtgacttcg atatgttctc tcaatgtcaa ttcttcatat aaagaaggag
    19921 tctctggaat atacccaatt tgtttgcgat attcttccgt atttttcttt aaggatagat
    19981 gattgatttc tatcgttcct tttgaagctt ccagcagacc gataatgtgc ttgatggtcg
    20041 tacttttccc tgctccgttc agtccaatca accctactat ctctccaggc tgtacatcga
    20101 aactgatatc ttttaacaca gggagatgtc cgtacccccc tgtgacatga tcgataatta
    20161 aactcattct ttttcctccg ccatttattt ttatttatta taccacgaag aaaatgacgg
    20221 tattttgttt ttttccttac aatagcttta acttatggta aagttaagct gaagtattta
    20281 ttctttatag aaaggatgtt ttatatggaa aactgtattt tttgcaaaat catcaatcaa
    20341 gaaatcccaa gctataaaat atatgaagac gacaaggtct atgctttttt agacatctct
    20401 caagcaacga aaggacacac attagttgtg cctaaacgtc atgtagcaga tatttttgaa
    20461 tatgatcccg aacttgcagg agaggttttt tcgcgtgtgc ctaagattgc tcaagcattg
    20521 gaaaaagctt ttccagaaat gaaaggtctt aatatcttga ataacaaccg tgaactagcc
    20581 tatcaatctg tttttcattc acatatccat ttagtccctc gctacagcaa agaggatgat
    20641 ttttctattc actttgtcaa tcatcaagat agttatggat cggaagaact aaaagctatt
    20701 caagaaacaa tcgtaaagca ggtgtcctgc gatgattaaa aactttttca aaggactgtt
    20761 atttggagct gctgctggca ctgtcggagg tctgttattg gcgcccagga gcgggaatga
    20821 aacaagaaag aaactgatcg aagaattgga agaagcaaca actctgacaa atgatttaaa
    20881 taacagcctg aatcatttta aaagcgcatt gatcgaaaca aaagaaacag ctgaatctgt
    20941 gattcctgct ttccaagaag caatcaaaaa agatatcgaa gattttaaat tccaagccga
    21001 accacgtatc acacaaatca atgaacaagt agaaaaacta aaagctgatc tgccagagta
    21061 tcctgaacaa atataataat gattattttc tcaacagatt taactatata agtgagaaaa
    21121 gtttgcgtat ttcctaggaa tgtgatgaaa ttttgaattt tgtttgaaac taccacgaca
    21181 acgtttttaa aggaaatggg atatggtata gttaata
//
LOCUS       DAXWLH010000041        20525 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXWLH010000041 DAXWLH010000000
VERSION     DAXWLH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.041"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="ACGE36_001101"
     CDS             complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="ACGE36_001101"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFF8383514.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="ACGE36_001102"
     CDS             complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="ACGE36_001102"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8383515.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8109..9077
                     /locus_tag="ACGE36_001103"
     CDS             8109..9077
                     /locus_tag="ACGE36_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8383516.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9095..10000
                     /locus_tag="ACGE36_001104"
     CDS             9095..10000
                     /locus_tag="ACGE36_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383517.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9997..10803
                     /locus_tag="ACGE36_001105"
     CDS             9997..10803
                     /locus_tag="ACGE36_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383518.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10960..11928
                     /locus_tag="ACGE36_001106"
     CDS             10960..11928
                     /locus_tag="ACGE36_001106"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFF8383519.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12245..12769)
                     /locus_tag="ACGE36_001107"
     CDS             complement(12245..12769)
                     /locus_tag="ACGE36_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFF8383520.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13056..13688
                     /gene="scm"
                     /locus_tag="ACGE36_001108"
                     /pseudo
     CDS             13056..13688
                     /gene="scm"
                     /locus_tag="ACGE36_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
     gene            complement(13698..14657)
                     /locus_tag="ACGE36_001109"
     CDS             complement(13698..14657)
                     /locus_tag="ACGE36_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8383521.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            14751..15932
                     /gene="scm"
                     /locus_tag="ACGE36_001110"
                     /pseudo
     CDS             14751..15932
                     /gene="scm"
                     /locus_tag="ACGE36_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
     gene            complement(16087..17073)
                     /locus_tag="ACGE36_001111"
     CDS             complement(16087..17073)
                     /locus_tag="ACGE36_001111"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8383522.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(17096..17662)
                     /locus_tag="ACGE36_001112"
     CDS             complement(17096..17662)
                     /locus_tag="ACGE36_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFF8383523.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(17676..18092)
                     /locus_tag="ACGE36_001113"
     CDS             complement(17676..18092)
                     /locus_tag="ACGE36_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFF8383524.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(18549..20483)
                     /locus_tag="ACGE36_001114"
     CDS             complement(18549..20483)
                     /locus_tag="ACGE36_001114"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8383525.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         5887 a         4440 c         3955 g         6243 t
ORIGIN      
        1 ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt
       61 ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga
      121 cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac
      181 ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat
      241 ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta
      301 atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga
      361 ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga
      421 tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga accattactt
      481 cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa
      541 gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg
      601 atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg
      661 ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg
      721 tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg
      781 cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga
      841 tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt
      901 caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag
      961 ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga
     1021 cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg
     1081 aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac
     1141 gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga
     1201 tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt
     1261 gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat
     1321 cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt
     1381 cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac
     1441 cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca
     1501 tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt
     1561 ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac
     1621 gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt
     1681 tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt
     1741 tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt
     1801 tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt
     1861 gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt
     1921 ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct
     1981 cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa
     2041 atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc
     2101 cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc
     2161 gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga gaaggagtaa
     2221 caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg
     2281 cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat
     2341 cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa
     2401 ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg
     2461 ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt
     2521 tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga
     2581 atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga
     2641 aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt
     2701 gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac
     2761 ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt
     2821 gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt
     2881 tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa
     2941 aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt
     3001 ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac
     3061 ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg
     3121 ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag
     3181 cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca
     3241 attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa
     3301 tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc
     3361 ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac
     3421 ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac
     3481 ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct
     3541 cacagaacaa cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt
     3601 ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac
     3661 tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg
     3721 caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt
     3781 ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt
     3841 tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga
     3901 tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca
     3961 agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg
     4021 gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc
     4081 cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag
     4141 cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc
     4201 ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat
     4261 cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg
     4321 gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac
     4381 gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga
     4441 tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac
     4501 gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg
     4561 gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca
     4621 ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta
     4681 cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac
     4741 cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa
     4801 agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt
     4861 tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt
     4921 ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc
     4981 ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt
     5041 catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag
     5101 tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc
     5161 cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt
     5221 tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac
     5281 cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt
     5341 tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc
     5401 atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg
     5461 cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt
     5521 gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt
     5581 gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg
     5641 cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa
     5701 cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta
     5761 ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga
     5821 tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt
     5881 cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa
     5941 gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac
     6001 ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga
     6061 tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt
     6121 gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat
     6181 tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac
     6241 gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag
     6301 ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt
     6361 cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt
     6421 ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat
     6481 caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt
     6541 ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat
     6601 tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa
     6661 cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga
     6721 ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa
     6781 atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat
     6841 aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag
     6901 tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg
     6961 aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt
     7021 ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc
     7081 ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag
     7141 cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat
     7201 tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta
     7261 agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac
     7321 tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca
     7381 agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat
     7441 atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa
     7501 accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta
     7561 tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc
     7621 aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct
     7681 ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta
     7741 attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct
     7801 atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta ctttagctac
     7861 tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat
     7921 gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg
     7981 agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac
     8041 ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg
     8101 aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag
     8161 gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg
     8221 tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat
     8281 tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa
     8341 acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc
     8401 tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga
     8461 tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac
     8521 ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac
     8581 tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa
     8641 actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca
     8701 aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag
     8761 atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg
     8821 gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag
     8881 acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa acagggatca
     8941 tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg
     9001 atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca
     9061 tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt
     9121 atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt
     9181 ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat
     9241 cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt
     9301 agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc
     9361 ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg
     9421 tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg
     9481 cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg
     9541 tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa
     9601 tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat
     9661 gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca acggctttgc
     9721 tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga
     9781 agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt
     9841 catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa
     9901 acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt
     9961 ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa
    10021 aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt
    10081 aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa
    10141 tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg
    10201 aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta
    10261 ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt
    10321 gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca
    10381 tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa
    10441 atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag
    10501 cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa
    10561 acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc
    10621 actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga
    10681 aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta
    10741 gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca
    10801 taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct
    10861 tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc
    10921 ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta
    10981 cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg
    11041 gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt
    11101 tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa
    11161 atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat gttgacttct
    11221 gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt ttcgaagtta
    11281 tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa
    11341 cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg
    11401 cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt
    11461 cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg
    11521 attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg
    11581 atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc
    11641 atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc
    11701 tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa
    11761 aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt
    11821 cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg
    11881 agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc
    11941 acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct
    12001 ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct
    12061 ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt
    12121 tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg
    12181 taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag
    12241 tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag
    12301 cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct
    12361 cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat
    12421 cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg
    12481 cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc
    12541 tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat
    12601 ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat
    12661 cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc
    12721 gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc
    12781 ctttttattt atcctactta ctttttccta attctaggat acctacttat atgttactac
    12841 acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa
    12901 aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat
    12961 tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata
    13021 ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag
    13081 tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat
    13141 acattcctaa agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag
    13201 gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag
    13261 acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg
    13321 aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt
    13381 caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg
    13441 tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag
    13501 atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta
    13561 aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg
    13621 cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt
    13681 ttttattgtc ttaattgtca aattaagtta gacagatcat caatacttac gtaactcaaa
    13741 aatacttcca aaggtgttcg ataatccagt gattttctag gaatattatt tcgtttagat
    13801 gcgacagatt gaataaaaaa ttcatctact gaattgaaat ccatagattt caataaacca
    13861 tctctacgta acaatccgtt agaattctcg tttaggcctc tttgagacgg cgttcctgga
    13921 tcagcgaaat aaatggcaat atcattgaca ttactgatct gtttccaatt tgaaaattcc
    13981 tttccacaat caaaagtgat ggatttgaat aggtttttcg gtacagattc aaaccattga
    14041 tttaattttt tttcaatatc aatcgcttgt ctaccacacg gtttcaatgt gatgataact
    14101 tttgataatc gttcaactaa ggtaattaca gcacttttat gtttcagacc tacgatagtg
    14161 tcaccttcaa ggtgaccaaa ctcatttgag aattggctat aatccttttc acgttcatga
    14221 atagagcggc ggaaagtttg ttttccacgt ctttcttgat gtccattcgg tttacgcttg
    14281 cctttcatcg gtaagtcaga agaatcaaat agcccctttt tgaacatacg ataaatggta
    14341 cgagcagaac aactaatagg aaacgctgca cgaccaacaa tcacatcggg tgtccatcct
    14401 tgaacaacct ttctttgaat atattctgat tgttcctcgg gtaaaacaag cggacgtcta
    14461 ccacagtttg atttgttttt cttatactgt tgataatatt ctaaggctga ttttccttgc
    14521 ttgaagaaca gatatacttt atggatcgtt tgtcttgaac gattcaagca atgggcagtt
    14581 ttagcaacag attgattcat tttgaaataa gactctatta ttacaagttc atccgttgta
    14641 agatgggtat aggtcatttg tactcactcc ttataattct ttggtcggaa ctattttgag
    14701 tgtagcacaa atgacttttt tagttgtcta gcttaatttt acaatcggcg ttggtaaata
    14761 tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag
    14821 tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat
    14881 acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt
    14941 ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc
    15001 ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga
    15061 ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta
    15121 cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt
    15181 catcaacttc aagcgacagt acagatacta cctctaccac aagtgatagc acggatacat
    15241 cagcttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg gacacatcag
    15301 cttcaagtga cagtacagat actacctcta ccacaagtga cagcacggac acgtcagctt
    15361 caagcgacag tacagatact acctctacca caagtgatag cacggacaca tcagcttcaa
    15421 gtgacagtac agatactacc tctaccacaa gtgatagcac ggacacgtca gcttcaagcg
    15481 acagtacgga tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca
    15541 gtacagatac tacctctacc acaagtgata gctcagattc atctaccact acaagtgata
    15601 gcacggacac gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct
    15661 cagattcatc aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc
    15721 atgcaagtgt ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta
    15781 ataaaacaag caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc
    15841 aatcgaataa ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca
    15901 cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac
    15961 gtcgctaacg gacgttgctc tttatttttg ctttttatca taaatacaag aactacaatt
    16021 caatttgtca aaaaacacgt agatttcctt ctgagaaatt tatttacgta tcgaaccttt
    16081 ccctattcag tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt
    16141 tcgaagagag gtggcaggta atttctttat ttccttcaaa gccttttctg tgtaagaggc
    16201 cgctaacttc ctagcttctt ccacgcctcc tgtatgttca acgattttgc gtacggcgtc
    16261 gacatcttct tctgtcattg ccgctttctt ctctaaataa ggtagtagct ttggtttgtt
    16321 tttttgcaaa gagtagatca acggtaaaga atagacccct tgacgcatgt cttctagtac
    16381 aggtttgccg atttcctggc tctcttttgt ataatctaaa atatcatcga taatctgaaa
    16441 ggccatcccg atagaaagtc cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc
    16501 actttcaaaa gcgccaacag aacaacttaa ttggaacagt tcagctgtct tacctgaaat
    16561 attttccaaa tattctttta cagaaagttc ataattatag cgatcatcca tctgccctaa
    16621 ttcgccattc aatacttttt ccatactgcg agaattcaat tggagacttt tcaaggaagt
    16681 ggagtaatca gacaatagtt taaaacagca gacaaataaa tagtctcctg catagacaga
    16741 aacgacatta ccaaactgac tacggatatt aggctgacca cgtcgcagat ccgcttcatc
    16801 aacgatatca tcatggatca gtgtggccgt atggagcatt tcgatagaag ctgccaaagc
    16861 gatggctttg tctcgatctt gctctggtcc aaattgtgag aacaatagct gataggcagg
    16921 acgcaacagc tttccgccgg catggatcgt ttctttgatg gcttgttcga caggtttgtt
    16981 tttcaggcgg acagtttctt ccatccgttt tagtgtttct gtcaattctt tacttaaagc
    17041 aggatagttt tcccacattg gatgtagctt cataaaaaaa ctccttgtgt gacgattatt
    17101 ttttggtaaa cattgggctc catttttgtt tggttccctg ctcataatct ttttggaatc
    17161 gttgaagtgt ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt gcaatccctg
    17221 ataaaatccc taaaactgaa aggactggca agtacaacat gactgtccat gattgtgcaa
    17281 tgaaagaagc catcaaaagc tgtccgacat tatgcatgaa accgccggcc gcactaattc
    17341 cgatgatact gacacgtttt ggtcctaatt gcttgaccaa taacattcca aaataactaa
    17401 gcaaagcacc actcatacta taaaggaatg tggaaatcgt tccgcctagc aaagtagtca
    17461 ggaatagtcg tagccaaagt agtaagaagc tgtcttgttt tttcatggta aaaatggcaa
    17521 cgatcgtgat tagattggct aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag
    17581 ggatcatatt ttccaataaa ccaatgatca ctccttgtgc aacaagtagc gaaatataaa
    17641 tcattttttg taatttactc atggtgtgtg aaggtctaat aaatcagact gtcttcgtca
    17701 cctccatctg ttgaacggac ttcgatcact aatttatgtg gcaaacagac gatggtttcg
    17761 ccatttttcg ttgcccagcc tcgacgtacg cagatctggt catcgcaatc tgcttctttt
    17821 atccgtactc gatcgccatc tatctcgatc aagttataat ctccgtgttc atctttgtaa
    17881 gtatatgtgt tgctctcttc tccagccatc aaagggaatg ttttgatttc tgtgccatcc
    17941 acacgcagta ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg
    18001 actggtaaaa aagaaagaac agtcaaaagc aaaataataa aaacatccca aggtttgagc
    18061 agactttttt cagcaaattc tttaaggctc atttttgttc tcctttttcc agattaactc
    18121 acccaatttt agcataaaaa caagaggttg tgacacctgt gtcttgcttt gagtaataag
    18181 aacgaagaat agaaaacagc tttccatgtt ttctaattct ttgggcttat taccgagaag
    18241 cagcaggtgg aacaccgttt ataacaagag gttgtgacag aagtgggcaa cttcaagaaa
    18301 taagaaggaa tttacgaaaa ttacttttca agtttttgtg aaatttcggc ttatttttcg
    18361 aagaagctac ttctggaaca ccgtttataa caagaggttg taacagaggt tgtgacacct
    18421 gtgtcttgct catggaggct tatctcgggc atcttattcc tttaatttta cataaaaagg
    18481 aggctgtgac ggtttgtctc agcccctttc tattgaaatc ttgcattcaa ataacgttct
    18541 ttatctattt atttcttgcc gtactgggaa cttggtctac cgtaccacca attacctagt
    18601 gtacgttgta gccatggaat tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc
    18661 atcaatgcga aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat gcctgataag
    18721 cagaacgtga tcaccaatac aatcgttgtc gcactgcata gccatgtgaa taatccagca
    18781 atcaatgcta atgccaaggc aatttcaaag aataccatga atttttggaa gaacattgct
    18841 acatcattgt ttggcatgat cacttgcatg atctttgtga accagtctgg tgacttctca
    18901 atcacagcca ttggttcttc accgtatgca taactcaaac caaaatgtgc agcttgagtc
    18961 gctgcttctc cggcacttgc ccctgctccg ctggcagctg tggttgcatc accagcagca
    19021 gaagcaccac tggcaacatc tgttacattg tattcccatg tccaagggaa cacactgttt
    19081 ccagtgaacc atgatccttc accaaaccaa gtacttggtt tgaacacttc accattccca
    19141 atgatttttt tggaagcttc aacgaaccaa actaaaccat agaaaatacg aagtggcaca
    19201 ctccataaga cattgccgta acgagaagaa tgtcctcgag tcacgttgcg atcatctttg
    19261 atatggaaaa actcatgcat taaatattgg aacatataat aaccagaacg aatatcaaag
    19321 aagtatttca aattcacgat atgtttcatt acgatagcta aaaatccgct taaatggatc
    19381 ttatcaaata ggttagctac cccccatttg gcaccaatag aaaccataaa tccttgatag
    19441 tttcctttaa atttatgctt ttctccgcct ttgatcgaag ccacgatatt cgctgcggct
    19501 gtgtgtcctg tttgttcagc agcttgaacg atctgcggtg tcggtgttcc tgggaattct
    19561 tcataaaaga ctaaatcacc aatgatatag atgtttttat cttcgtagcc ttttgcctgc
    19621 atatattcat tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg
    19681 tcagaagttc ctttgacacc agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg
    19741 tctttcaatt tgatatgatc agcagccact tctacgattg gtgcattcaa taataattcc
    19801 acgttgtgct tacgcaaata gcgttcagct tttgcagcat catttcgatc caacatattc
    19861 aagatcgtcg gcattgcctc tacgaccatc agtgtgattt cgtttggatc taatttataa
    19921 tctttggcta ggcgattttt ccagtcgatc agttccccaa tcatttcaat accggtaaat
    19981 ccagaaccac agacaacgaa agtcagcatg gctttacgca gttcatcatc tggttccaat
    20041 gcagcttttg ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac
    20101 caaagggtga atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg ctctccgccc
    20161 attccaagga tcagctgatc aaaaggatag gaacctaatt tggtcttgac gatttttcgt
    20221 tctttatcga taccgattac agtgtctgtg acaagttgta cattcttttg acgagcaaac
    20281 aaatgctgca gatcgtattg gattgcttct ggttctacac gtccgccagc tacttcatgc
    20341 aactcggtca tcatagtatg gtacgaatga cgatcgatca gagtaattgc tacatcttct
    20401 tttttcagtt tctttgccaa gtattttgta gctgaaacac cggcatatcc tgctcctaca
    20461 acgacaatat tttgctttgc cattgatcat ccgctcctta tagactatct atataatgaa
    20521 tatat
//
LOCUS       DAXWLH010000042        20313 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXWLH010000042 DAXWLH010000000
VERSION     DAXWLH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.042"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..3386
                     /locus_tag="ACGE36_001115"
     CDS             <1..3386
                     /locus_tag="ACGE36_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8383526.1"
                     /translation="TTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAEVI
                     SDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITLEI
                     SSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIEEE
                     SSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVLDH
                     QGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDVYL
                     NARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINMGY
                     VGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPNGH
                     KKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQNN
                     LSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSSDE
                     KGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVGTS
                     PTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTTFQ
                     PNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAAAW
                     KNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIKEL
                     PVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLTDH
                     GDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVTTS
                     DDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQTTN
                     EKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNVWT
                     LTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLKPG
                     TYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITNQA
                     KVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3389..4810
                     /locus_tag="ACGE36_001116"
     CDS             3389..4810
                     /locus_tag="ACGE36_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8383527.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4807..6684
                     /locus_tag="ACGE36_001117"
     CDS             4807..6684
                     /locus_tag="ACGE36_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8383528.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6776..7618
                     /locus_tag="ACGE36_001118"
     CDS             6776..7618
                     /locus_tag="ACGE36_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8383529.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7770..8645)
                     /locus_tag="ACGE36_001119"
     CDS             complement(7770..8645)
                     /locus_tag="ACGE36_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8383530.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8852..9883
                     /locus_tag="ACGE36_001120"
     CDS             8852..9883
                     /locus_tag="ACGE36_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8383531.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9972..10277
                     /locus_tag="ACGE36_001121"
     CDS             9972..10277
                     /locus_tag="ACGE36_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFF8383532.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10456..12471
                     /locus_tag="ACGE36_001122"
     CDS             10456..12471
                     /locus_tag="ACGE36_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8383533.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELWSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12473..12757
                     /locus_tag="ACGE36_001123"
     CDS             12473..12757
                     /locus_tag="ACGE36_001123"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8383534.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12783..14156
                     /locus_tag="ACGE36_001124"
     CDS             12783..14156
                     /locus_tag="ACGE36_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8383535.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14239..16254)
                     /locus_tag="ACGE36_001125"
     CDS             complement(14239..16254)
                     /locus_tag="ACGE36_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFF8383536.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16276..17490)
                     /locus_tag="ACGE36_001126"
     CDS             complement(16276..17490)
                     /locus_tag="ACGE36_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8383537.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17711..18655
                     /locus_tag="ACGE36_001127"
     CDS             17711..18655
                     /locus_tag="ACGE36_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8383538.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18951..19895
                     /locus_tag="ACGE36_001128"
     CDS             18951..19895
                     /locus_tag="ACGE36_001128"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8383539.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6934 a         3722 c         3871 g         5786 t
ORIGIN      
        1 cgaccacagg gaagaaactg aaagttattt tcatgctgat aatattgagt ttatcaaact
       61 ttgtgccatt atctgcaata gcagacacta cagatgatcc aacagtttta gaaacaattt
      121 cagctgaagt catttcggat cagtctggaa aaaaagcact gaacatcaag ctaaatgcga
      181 ataacaccag tgctgaaaag atagaaaaag aaattggtct agtcgaaaat tacttaagtg
      241 atgtggaaag aaaagaagga gatggctatg cttatcaggt aaatagcggg aaaattacgt
      301 tggaaatctc atcaaacact aaacaaacta tcgatctgag ttttccaatc gatccagcac
      361 tttaccacag ccaggcaaac aagctgatcg tcgataataa agaatatgac attattgatg
      421 agacagaaaa taagaaagat acagatgtgt cagtaccaaa gccagacgaa atagaagaag
      481 aatcatcaaa agaaaacgaa aattctgtca gcccatttac attgcctaca ttatccttgc
      541 cagctgtgag tgtgccatct aatcaaacga ttcctacaga atatacaaca gatgatcagg
      601 gcacttatcc taaagccagt tggcaaccta caggaaatac aaatgttctt gatcatcaag
      661 gcaataaaaa cggaacaaat caatgggatg gtataaattc ttggaatgga gatcctaatg
      721 atcggaccca ttcgtatatc gaatatggag gaaccggtaa tcaagcagac tatgcgatac
      781 gaaagtatgc aaaggaaaca agtacacccg gattgtttga tgtttatttg aatgctcgtg
      841 gaaatgtaca aaaagatatc acgcctcttg atctcgtatt ggtcgtagac tggtcaggaa
      901 gtatgaacga caataatcgg atcggtgaag taaagattgg tgtcgatcgt tttgtcgata
      961 ctttagcaga tagcggtatc acagacaaaa tcaatatggg atatgtcggc tactcaagcg
     1021 aaggatatag ctacagtaac ggtgcagtac agatgggttc atttgattca gtgaaaaatc
     1081 aagtaaaatc cattacacct tcacggacaa atggtggtac ttttacacaa aaagcactaa
     1141 gagatgcagg aagcatgcta tccgttccaa atggacataa aaaagtgatc gttttgctga
     1201 cggatggtgt accaacattt tcctataaag tacagcgggt acacgcacaa tcaagcagca
     1261 attattacgg aactcagttt tctaatacgc aagatcggcc gggaaatact tctctaatct
     1321 caagaatcta tgatgcacct gaccaaaaca atctatccag aagaatcgac agtacgttta
     1381 tcgcaaccat cggagaagcg atggcactca aagaacgagg aatcgaaata catggtcttg
     1441 gcatccaact tcaaagcgat ccggcagctg gtctctcaaa agcagaagta gagtctcgta
     1501 tgcgacaaat ggtttcatca gatgaaaaag gcgatcttta ctatgaatca gctgatcatg
     1561 caacagatat ctctgaatac ctagccaaaa aagctgtaca gatctcagca actgtaagca
     1621 atggacaaat aaatgatcca atcgcagaac cattcattta tcagcctggt acactttcag
     1681 tcaagagtgt ggggacaagt cctacaacgg tcactccatc tatttccata gaaggaaata
     1741 ccatcaagag caatcagatc tatttaggaa aagaccaaga aatccaaatc cattaccaag
     1801 tgagaatcca aacagaaaat gaggacttcc atccaaattt ctggtatcaa atgaacggca
     1861 ggacaacttt ccagccaaac attgatacca atgaattagc tgaattcggt ataccatctg
     1921 ctaaagctcc cggagtcagt cttcacatca aaaagttatg ggaagaattt gacaacaatc
     1981 tagctgatcg tccagatcaa gttacttttg agattcaacg ggaacatacg acaaatgctg
     2041 cagcttggaa aaacggatat attcgaatca ttaaaccagc taaagataca acaaatacgt
     2101 gggaacgtgc agacattgac aaattatctg caaatagcgg agaaagttat caagagatat
     2161 tatcactacc tcaatacaat aatcaaggtc aagcattcag ttaccaaaca atcaaagaat
     2221 tacctgtacc aggatacgat tctcaacaaa tagatgcaat gacatggaaa aatactaaac
     2281 aattcacacc gttaaacttg aaaataacga aaaattcctc tacaggtgaa aaggatctta
     2341 ttggcgctgt tttcaaatta acaggagatt ctattgatac tttactaaca gatcatggcg
     2401 acggaaccta ttctcttcca gaaaatgtca aattgcaaaa agaaatgacc tatacgctga
     2461 cagaaacaaa agctccagaa gggcatggat taagcaaaaa gactacttgg gaaatcaaga
     2521 tcgcttctga tggtacggta accattgatg gaaaaacagt cactacttcc gatgatacga
     2581 tccagttgac tattgaaaat ccttttgttg aagttcctgt agcagtacgt aagtatgcga
     2641 tgcaagggac ggacaaagag ataaatctta aaggagcagc attttcccta cagaaaaaag
     2701 aagcaaatgg tacttatcag ccaattgaca gccaaacaac gaatgaaaaa ggtcttgcca
     2761 gttttgattc actcacacct ggtaaatatc gagtcgttga aacagctggt cctgccggat
     2821 atgatacttc gccgggaaat tatgaattcc aaatcgataa atatggaaaa atcatttaca
     2881 cgggaaaaaa taccgagatg acaaataatg tatggacgct cactcatcaa aatcgactaa
     2941 aagcgtttga tctaacggta cacaaaaaag aagacaacgg acagacatta aaaggagcaa
     3001 aattcagact gcagggacca gaaatggact tagaatcgcc aaaagatgga caagaaacag
     3061 atacctttct attcgaaaat ttaaaacctg gaacttatac gctgaccgaa acttttacac
     3121 cagaaggata ccaaggtcta aaagagccag ttactatagt tatacacgaa gatgggtcaa
     3181 ttcaagtgga tggacaagat catgaatctg ttctgtcacc aggagccaaa aacaaccaga
     3241 tttctttaga catcacgaat caggcaaaag taccattacc tgaaacggga ggaattggcc
     3301 gtttaggaat ctatctagta gggatgattg gttgtgcgtt ttctatttgg tatctttttt
     3361 tgaaaaaaga aagagggggc agctaaatat gaaaaaactt ggttggctta gtatgtgtct
     3421 cttcttgtta ctatttaaac cagcttttac tcaggtagca acagaaacag aaacagaaat
     3481 ggttcagatt actttacaca aattgctttt cccaaacggg caactgccga aaaatcatcc
     3541 aaatgacgga caagaaaaag ctttattaca aacgtatcga ggattaaatg gtgtcacatt
     3601 ccaagtttat gatgtcacag attcttttta ccatctacgg gaaaagggca aaacggtaga
     3661 agaagcacaa gcagagatcg caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc
     3721 aacaactaca actcttaaca acgaagatgg tatcgcttct ttttctctgg ccgctaaaga
     3781 tcaagaaaaa agagataaag cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa
     3841 agaaaaggca gagaatatgg tagttgttct tcctgtacat ggacaaaaca atcaaaaact
     3901 ttcaactatc catttgtatc ctaaaaatga agaaaacgac taccctgatc caccttttga
     3961 gaaggtatta gaagagccta gaaatgattt tacgattggt gaaaaaatca cttattcctt
     4021 gcatacgaca attcctgtaa atatccttga ctatcaaaag ttcgaattgt cagatagtgc
     4081 ggatgaagca ttaacgtttt tacctaatag tttaacgatt tcatcgaatg gagaaaagct
     4141 gacagaaggc tttgtcatac acaagaaacc tcacggattt gatgttttat tttcgatccc
     4201 ttcgttggaa aaatatgctg gaaaaaaact gaccatttct tatcagatgc agctaagcag
     4261 tacagcacag gcgaacaagg aaatcaacaa caacggaaca ctggattttg gttttggtgt
     4321 cagtacaaag aaagtctctg tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa
     4381 taaaccagat aaacgattag ctggcgcagt attccttatt aaaaacaaag caggaaatta
     4441 cctccagcaa acagccaacg gatacaagtg gacaaagaac gaatcagatg cgcttcacct
     4501 gatttccgat aaaaatggcg ctttttcaat ttccgggttg aaaacaggaa gttatcgatt
     4561 aaaagagatc gaagcacctt ctggttatat tttaagtgaa acagaaattc cgtttaccat
     4621 ttcaactttt ctttctgagg ataaagaggc ggacagtata ttgaaagtag tcaataaaaa
     4681 agaaaatagc cgtccatttc ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt
     4741 tgttggtatg gtattcgcaa gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt
     4801 gaaaaaatga aaaatcataa aaaaataaac gttatgttag gagtcctttt ccttatttta
     4861 ccattactca caaacagctt cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa
     4921 gtcatccttc ataaaaagaa aatgactgat ttacccgatc ctttaatcca aaacagcggg
     4981 aaagaaatga gcgaattcga tcaataccaa ggattagccg atatttcatt ttcagtttat
     5041 aacgtcactc aagaatttta tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa
     5101 caagcagtcc agtctttgac tcctggtaca ccagttgctt caggaacgac agatgctgat
     5161 ggaaatgtca ctttatcttt acctaaaaaa caaaatggga aagatgcagt ctacacgatc
     5221 aaagaagaac caaaagacgg agtgtcagct gccgcaaaca tggttttagc tttccctgta
     5281 tatgagatga tcaaacaagc agatggctct tataaatacg ggacagaaga actagatact
     5341 atccatctct accctaaaaa tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc
     5401 ggtactgccg aaaacgaagc actaaatgga gcagaattta ttatttctaa agaagaagga
     5461 acaccaagcg tcaaaaaata catccaaagt gtcacagatg gattgtacac ttggacaact
     5521 gatcaaacca aagccaaaca tttcattact ggtcattctt atgacatcgg caacaatgac
     5581 tttgccgagg catctattga aaaaggccag ttgatcgtta atcatttaga agttggaaaa
     5641 tataatttag aagaagtaaa agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc
     5701 acgccttttg agatcctggc aaatagccaa acaccagtag aaaagaccat caaaaatgat
     5761 acgtctaaag ttgataaaac aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa
     5821 aaaattcaat atgagatttc tgtcaatatc ccattaggta tcgctgataa agaaggaacg
     5881 caaaacaagt acacaacatt caaacttatc gatactcatg acgctgcttt aacatttgat
     5941 aatgattctt caggaacgta tgcttatgcc ttatatgatg gaaataaaga aatcgaccca
     6001 gtaaattatt ctgtcactga gcaaacagac ggattcacgg tttcagttga tccgaattat
     6061 attccttcat taactcctgg cggtacattg aaattcgttt actatatgca tttgaacgaa
     6121 aaagcagatc caaccaaagg attttctaac caagcaaatg tcgataacgg gcatacaaat
     6181 gatcaaacac caccgtcagt cgatgtcgtt actgggggca aacgatttgt taaagtagat
     6241 ggtgacgtta catcagacca aacacttgct ggagcagaat tcgtcgttcg tgatcaagat
     6301 agtgacacag cgaaatattt atcgatcgac ccatccacaa aagccgtcag ctgggtatcg
     6361 gcgaaagaat cagcaacggt ttttacaacc acaagtaacg gtttaatcga tgtgacaggt
     6421 ctaaaatatg gcacgtacta tctggaagaa acgaaagcgc cagaaaaata tgttccatta
     6481 acaaaccgtg tagcatttac tatcgatgaa caatcttatg taacagcagg acagttgatt
     6541 tctcctgaaa aaataccaaa taaacacaaa ggtacacttc cttcaacagg cggtaaggga
     6601 atctatgtgt atatcggtgc aggagtagtc cttctactga ttgctggact gtactttgct
     6661 agacgcaagc acagtcagat ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt
     6721 acgcaaatgc tcctttttct tttattatca aacagtcatc acaaggagaa cacaaatgaa
     6781 accaaagaaa aggaaacttc tagatctgct gatgcttttc ttgcttctct caggaatagg
     6841 gatactagct tatccatttg ttagcgacgc gctgaataat tatctggatc agcagatcat
     6901 ctctcattat cagcaacaag cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga
     6961 aaacatgaca aagaaaaacc agcaattagc aaaagaaggt ggaaatccag gtgcagatcc
     7021 ctttactaag aaaaaagaac ctgcgaaaaa ggatagaacc tattttgaga aacatacgat
     7081 cggtattttg acgatccctg agatcaatgt ccatttgcca atttttgatg aaacgaatac
     7141 tgtgctgcta gaaaaaggtg cctcattatt ggaaggaacg tcttatccta cggggggaga
     7201 aaatacacat gccgttattt ccagccatcg aggactgcct caagcaaagt tgttcacaga
     7261 cttaccgaaa ctgactttag gtgattcttt ctatatcgaa atcaatggac ggacactcgc
     7321 ttatcaagtc gatcagatca agacggtaga accgactgat acaaaagatc tgagaatcga
     7381 aaaagggcag gatttcgtga cactcctaac ttgtacacct tatatggtca acagtcatcg
     7441 cttacttgtc agagggcacc gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca
     7501 gaaagtatcc gagaacaaac gatttttcct gtatggattg ttagctgcat gtatactgat
     7561 tttcctcctt cttatatatc tttttcgtca atcgttcaaa ataagaaaca gaagttagct
     7621 attcctcatt tctataatat atctattcga atcgttccgt aaacaaacag gctgaggttt
     7681 gaaaaagaaa acttcagcct gtttttatga tgatatcaaa gagagcctat caaacactat
     7741 ctttcttatt actaaatgag gatcttttct taagttttcc gatattcttt aagaaggcct
     7801 ttttgcatca attcttgttt gtttttcaag ttgcggatat tctcttcaaa attttgagaa
     7861 tatatcactg agaataacgt atttaatacg taatcgaagg caatttgaga ggagaaagtc
     7921 gctactttag caaaatcata ttcctcctgc gatataacaa gacacatatc agcctgtttg
     7981 gccatttctg aatgctgatt tccggtaatc agcaaggtag gggcctctac atgcttcaaa
     8041 taggtcatta ttcgttcata atgatggatg cgaccgctgt agctaataaa aatggcgcaa
     8101 tcctcgctag tcaaattggc tgcattccaa gaagaatcac tgtattcttc agccatgatc
     8161 aaaaacttat tcaacttgac cattttattc tggaatttcc ttgccgtgat ttgagaatca
     8221 ccttttgcaa aaagaaagat tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa
     8281 tcttcatgtt caatctgcaa ttgtgctttt tttattgact ggatcgataa atccgccatt
     8341 ttctttgcaa tttgctgagt gctatcttca cttgaaaaag gaaaattagg atcaatcgtt
     8401 tgattgaact gagcaatcaa ttgatgaact tcccgcccca gctcgaaacg gaattcttta
     8461 aatccgctga atcccatttt tttgcataaa cgaatgatag ctgaatggga agtatacgta
     8521 gcttttgcta attcttcaat cgtcatgtag actgcagctt cgatattcct acggatgtaa
     8581 tcgacaatcc gtttctctgt cgtggtaaac aactcttgtt ttgttagttt ttcttcaata
     8641 agcatgatct gacctccaaa ttctatctta ctgataaaca cttggttccg ctactatctt
     8701 tttgattttt atccttactt tgaacattaa tttcaccaaa ccgaatgata acaagtgttt
     8761 ttctgaactt ttttaccaga atagctatta taaatacttt ctgcgctttt ctaggatata
     8821 ctgaaaacat caataaaagg aggcaccact catgcttaca atcgcttatc ttggcaatgg
     8881 aaagagtaca aatcgctatc atttgccttt ttcaagaaaa cttacgaaca aaatcaatat
     8941 caaaaaaatt ttttccccat ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac
     9001 tgatcagctg tctgatcttt gggatgatcc tgagatccaa ttagttgtca tcactacacc
     9061 tagtcaattt cattatgaat atggaaaaat ggcactcgaa catggtaaga atgtactagt
     9121 agagaaacct tttgcagaaa cgtcaaagga agcagaagag ttatttgcat tagcaaaaga
     9181 aaaaggtcta tttatccaat gttaccaaaa cagacggttc gattctgact tcttgacagt
     9241 ccaaaaagta atcgaaagcg gaaaattagg ggatatttta gaagtagaaa tgcattatga
     9301 ttatttcaga ccggaagtac cagaaaatac gcatgaattt tctactgcga acagctattt
     9361 atatggacat gcttgtcata cagtagatca agttctttct tatttcggcg aaccagatga
     9421 tatcagctat gatgtacgtc aacttttagg gaaaggacga atgaatgatt acttcgatct
     9481 ggattttttc tatggaaata tgaaaatttc gataaaatcc agttatttcc gcatcaaaga
     9541 acgtccaagt ttcgtcgttt acggaaaaaa agggatgttc gtgaaacaaa caaaagatcg
     9601 acaagaagaa catctgaaaa tgttttacat gcctgatcat gaagactttg gtatcgattt
     9661 gcccgaacat tatggtgtat tgacctatat agatgaacaa gggagttatc atgaagaaaa
     9721 agtaatttcc gaggttggtg attacagccg tgtctacgaa gggatttacg agacattagt
     9781 taatggcgca gaaaaagtca tcaaagatga agaaacaatc ttacaaatga aaattttgga
     9841 agaaggaatc aaaagcattc aagaatcaga aggaaaaata tgacatattt gtaagaatcg
     9901 ggtttgtttt ttgacgcata aactgtctta ttgtacgatg aaaaagtata gaactactag
     9961 gaggaaccaa catgacaaat tatgacggtg aaaaacacaa acgattaaga ggttcaatgg
    10021 cccctaaaac agaagaagaa gtggctttag caggaactaa ttctggtgaa acagaagaca
    10081 aagaagaatc attagctggc acaaactctg gtgaaacaga aaataaagaa acagctttat
    10141 ctggttcaaa ttctgtagaa gaggaaacaa agcctaatga gcccaatcac tttgaaaaag
    10201 atatcgaaaa agcggttgaa gatgtcaaag aaaaatttga agaactgacc tcaaaaaata
    10261 aacatgatca tcattgatcg gacgaaatca attcataaaa gcaggagaca tttggtcacc
    10321 tgcttttttt ctgtatccat tcgatagaaa tcgttcggta cagtcgtaaa aaaaatccaa
    10381 ctgtaagttg taccaaacac ccctcgattt aaaagcgcta ccatactaga atataagtag
    10441 gaggtgaaca gcgctgtgaa ctacaaaatt agttatctcc tgaaaacacc aagagaaatc
    10501 agtctagaaa aagcagcaga gatcatcgaa gtaaatacaa gcgaggcgat caaacaatta
    10561 actccttttg ccgaatatgt ggatgatacc cgtatccgta taaagaaatt ccgagcaatc
    10621 gactgggcag aaaaattttt ggatattact cgatttgaag ctgtgtatac cgaacaagag
    10681 cgaagagatc ttctttattt gatggtttat tcgactgcaa cgtctgtttc tgtttttcat
    10741 tttcaagatt ttcttcaggt ttctaaaggg acagtcctgt cagatatcaa aaagataaga
    10801 aatgaattag aagtggaaga ttgtcagctg tcgtatacta ggaaaactgg ttttaggctg
    10861 ctagggtcag aagcaatatt aagaagagaa gcaaaaaatc gcactgctcg tttgatgcag
    10921 tcacttgctg gaagatttgg gctcacgtat ttaaccgcaa ccctttccat tgattattac
    10981 gccaaagtta gagatttgct agataaaacg atttcactat cagatcttcg attcgcacca
    11041 aatcgaatgg atgaattagc ttattatttg agtttgacaa ccattcgaat tggaaaaaca
    11101 tccatggaac tggtaaaaga tgctgaactg ttggcttctc ttgcagtcac tgacattagt
    11161 cgacaagtat tgactggcct tggctattcc gaacatcaaa attcggagat tatatttttc
    11221 tctctttgct tagcatcgat cgttgaagga gatgtccagg agcctgcact tgattttttg
    11281 ctggaatgtt cttcagaagt catccatcgc atggaattcc tgatggccgt agaatttgct
    11341 tcgtttcgtg aattattgat gaatgtcttt tatcacttgg tccctgccta ttttcgtatt
    11401 tcctattctt tttatttacc taatgtgatg atcgatcaaa ttaagcatca atatgcttcc
    11461 atctacgaaa tgacaagaaa agcattactt ccgttagaaa gacgaatagg gaagagcatc
    11521 cctgaagaag agattggttt tttcactata ctatttggcg gagagatcag aaaagcggac
    11581 gcagaagaaa gaaatagaaa aataagagcc gtgatcgtct gtccaagtgg cattagctct
    11641 tcattgatct taaaaagtga acttcagcaa ttattcccga tgatcttttt tacagagact
    11701 aattcagctg accgcttaca cgaggttgac gaaacttctt acgatattat tttttctact
    11761 gtacctctca cacttactga taagaaaaag tctctctatg ttctgcatcc aatcatgact
    11821 gacttggaaa aaaatcagct gatcaatcaa gtacaaagag attggctgat accaggattt
    11881 tcaatgcctt ctgcccaaga attgtggtcg gcgctactgc cttatatcga attaaaagaa
    11941 ggcgtagacg aagaaaagct ctatcaagta ttaaatcgta aaatgaacaa acttttagaa
    12001 aagaaggagg atcaacgtcc tatgctatct gaattattaa cagaagaaat gatccaatta
    12061 tcggatcaac caaaaaactg gcaagaagca atcacatttg ccgcacagcc gctattatca
    12121 gaaaataaaa tagaagaaag ttatatcaaa gctatgatcg agcgcgtgga gcagtatggt
    12181 gcgtttatcc atattggcga tcacatcgca ttacctcatg ctcgtccaga agatggcgta
    12241 aacgaagtag ggatgtcttt gctgaaactt caacaacctg tggatctgct tgataatcca
    12301 gcccatccca ttagtttgtt catctgttta gctgctatcg ataatgaggc acatcttcgg
    12361 gctttggcaa atttaacaaa gctactttct aataaacaaa atcttcaaga tttattacac
    12421 gcatcaacaa aagaagaaat attagcgatc ataagaaaag gagacgaatg atatgaaaat
    12481 ttttgcagta tgtcaaagtg gattaggaac aagttttatg gtagagatga acatcaaaca
    12541 agcacttgca gatcttggag cagatgtgga cgagtttgaa gtaagtcata cggatgttgg
    12601 aagtgtatct agcgatatgg ctgattattt cttcattgag cattctttga aagatacgct
    12661 aacttctata cctgatgaaa agctcgtacc tttgcaatcg atcattgaca gcgaagaagt
    12721 aaaagaaaaa gtaacgaaga tcttgtcaaa agaataaaaa tgattcaagg gaggaactat
    12781 caatggataa attgttgaat ttgtttgtgt caatcgtcag tcagccctcc atcctcgttt
    12841 ctttgattgc actgcttggt ctcgttcttc agaagaagaa attttcagaa gtgatccaag
    12901 gaactatcaa aacatttgtc ggattcctag tactgatcgg cggtgctggt ttaatcagta
    12961 attcactaac cccttttgct tcgatgttcc aaacagctct gcatactcaa ggagtagttc
    13021 ctagcaatga agcagtagtg gcgattgctt tgcaacaata cggtacacct actgctttga
    13081 tcatgctagt agggatgatc gtgaatattt tacttgctag attcacaaga tataaattca
    13141 tttttcttac aggtcaagca atgctttatg tttcttgtct aacagcagtc attttagtaa
    13201 gcgcggggtt tactgcaaat ttgtggatga tcttattagg cggggtcttc gaaggaacac
    13261 tattaactat tacgcctgct ctttgtcaac cgtttatgaa gaacatcact ggaaacaatc
    13321 aagttgcgtt agggcatacc ggaaatatcg gttatgcttc tagtggatgg atcggaaagt
    13381 tttttggaaa caaagaccgt tctacagagg acataaagat tcctaaaagt ttcagctttt
    13441 tgcgagattc tactgtatcg atcatgctct tgatgtcgat cgtatatgtc gcacttgctt
    13501 tgttagctgg tcctggattt gttgaaagag aactaagcaa tggagacaac gctgtaatct
    13561 ttgcactgat ccaagctgga acattcacag ctgggtttgt tatcgtcctt caaggggttc
    13621 gaatggtgtt ggcagaaatc gttccagcat tccaagggat tgctaaaaaa ctcgtacccg
    13681 attctaaacc agcattagat gttcctatcg tttttccata tgcacctaat gctgtattaa
    13741 ttggcttttt tgttagcttc tttgtaggaa cactatccat gtttgctatg ataggcttgc
    13801 aaactacagt tatcattcct ggggtagtag gacatttctt ctgcggagct gcttctggtg
    13861 tcttcggcaa ctcgactggc ggtagaagag gggcggtatt aggttctgct ttcaatagct
    13921 tattgattag ttggctgcca ttatttatcc ttccagtatt aggagatctt aaaatcgctt
    13981 cttctacatt tgccgatact gattatctag taccaggact tgtacttggg aaactaggag
    14041 aatacggtca aatagccttg acagttggaa tcatcgcttt ctttttactt gtcgtattat
    14101 ctagctttct ttttgccgga aaaagaacaa acattgtttc taaagaacag aattaagcaa
    14161 acttcaaaat aaaaattgct tggacaatat cagaagaaga tttaaagtct tctgatatgt
    14221 cgcaagcagt tttttgtttt atgcatttat ttttgaatag cttgttaaga caagtggctg
    14281 ttttgctaga agaggataac ttttaccatt cattttcact gttccgcctg ttctatcaga
    14341 agtaatcacg atcttatctt ttgttttttc aagcgaaatc tttccatctt tgataggtag
    14401 cgtacaattc agtgtttcaa atgaatctag attaggttgt atcataaatt cttcataacc
    14461 tggctttgtt ggatacaaac cgataaaata gcgtcctaaa agatagattg ggcttgctcc
    14521 ccatgcatgg cataagcttt ttccataagg atctccatac atactgtact tttcatttcc
    14581 tgtttgagta gggtcgtatt cttcccagaa agttaccgca tcatgagata acatccctcc
    14641 ccaataatca agaatcgttt ggtagacgat attcgtctgt ccgagtttgc ataaagcatc
    14701 ttgttcatag aatttgaaat atggagtagt aatctgtgtg atagtgtcat tcaataagac
    14761 attctttaag atcgattgct gttggtcttt gttagcaaaa tcgaataaaa cagcgaaaat
    14821 attcgcatga cgcgtcacat ttcttctccc agattcatag caatcgataa aagcgccaag
    14881 ttcttcatcc caaaagtagt caaataggtt ttgtttcaat agttgatatt tttcttgata
    14941 gagatgagta tgataatttt cgttctctcc atatgtttca gctaagatat ctccgcaagt
    15001 gatcatggaa tgataggctt tcagtaataa gacttgttct gcagcaagcg ttccttcttt
    15061 atccatttca gaccaatcaa taaatatcca atcttttgct ctcccataaa taaaccctaa
    15121 ttcattcgtt tgctccatca agtatctcat taatgattgc atcttagggt agacgatgcg
    15181 taaaaattca tagtctttcg tcatcatata gtggttttcg ataccaatca accacaagat
    15241 cgaatagtca acgatagtat tcatatgctg cttgatttca ttttgtcctc ttaatgctaa
    15301 tatcgttcgt ttattgattt cctcgtcaaa aaaaagatat tgattgatca tgaatgattg
    15361 atacgcatct ccagaccaaa tccagcgatc tcgtttgata ccgtcgataa aaaacaaatc
    15421 actacataac ttgaaggttt cttctgatac tgaccagatc tgattcagta aagaagaatc
    15481 gcttgaaaaa gcagcttgtt tcgaaagaga taaaaattca tgaaaaactt gaagttgaac
    15541 cattccagta gaaacctctg gtataaacaa atagcgaaaa gcgcgtttac gagctttcat
    15601 ttttcttgtc attcctattt gcttatagta gcaataatcg atatccatag cttctacatc
    15661 ggactctcca taacatagag tgatagattc gatatggtca tccagagata ttagttctac
    15721 acagccattc actgctcgcc caaaatcgaa taagattcct ccgccaatct ccgtttcagt
    15781 gactggatgg acgcattctt tttgataata gatttgattt ggatcttttg cgatatcgac
    15841 ataaagcgga ctccagccag ctggatattc ttcaataaaa ttacttgctg tccagccaat
    15901 atctgatttt acttcgtctc cctcgataaa catcgctggt agccctgatg tatgtcctac
    15961 aaagatacga atttttgctt taccagctgg acaagtcagc cattttccac atggatgttt
    16021 ttgtccatta atttctacat acccaactcc ttggatcgtc actttaaaca tggaaggttg
    16081 atcaagaaag taatatctct taaacttgac gttccgatgg caatcatcca tgtaccagta
    16141 cgctggccag tcaaaacctc gttcttcccg ttggaaattt tgtagcagtc catggcggat
    16201 ctcaaagtct ccaggatacc aaagccatct atctttatgt ttttcatttg tcattttaca
    16261 cgacctccat caaatttatt ttgttgtcaa ttgttctttt atttcttcat ctactcttat
    16321 cactgtcttt ctattcgtcc tgtgtactac tagaaggcta ataagcgcgc taatcaacag
    16381 caagatactg ccattgacga aaacagcaag tgttttactc ccaagagaag caaaagagag
    16441 aaagagcaaa ccatatggtg caatgaaagt acctaaatta gcgccaacca ataagattga
    16501 agtattgaaa ctctccgcgt acttgggcgc ttggatactt acttggttga ataagtaagg
    16561 aatgaataac gaaaaagaaa ttcctgttag gatcgcacca aacccaatca catacaggtt
    16621 tatcacagtg gcaatcaata caaagccgat tcccatcagt agtaaagaaa gtgtaagcat
    16681 atagcgatgt gcttttgtct gaagccagcc aaaacaaact ccggcagcca tacttgccaa
    16741 ttgcatgatt gttaagatat tcgctgcatt agtttcgttt cctagagaat aagtcgtcag
    16801 gactgtggct aatttaatcg atacagcatt atataccacg atgaccagca agagaaaaat
    16861 accataacta atcattttag gattcgtttt gattacttgt cttgttgggg ctgtgccatg
    16921 tgattgaagt tcagttgttt caggtgtagg aacaaacaaa acaaacaaga caagaatagg
    16981 taagctgatg gcgtagacta aaaaggtata ttgccaaccg aactgtacta gctgtcctgc
    17041 aacaaatgtc attaaagctg caccaaatcc ttgaaatgca ctttgaaaac caatcataga
    17101 ggctcgttca tctccgtgat aaaaggcagc aatcatgctg actgccaaag aattgatcaa
    17161 tccaaagcca atacctagtc cagctcttga agctaagaca atactgtaac tttgtgagaa
    17221 gagggggagg ataccataaa tcgatgcaat cactaatccg atgatcacag tttgtttttg
    17281 tccgattttc ctagcaataa aactactaat caaaacaaaa atagtgacca taaaagaggg
    17341 agcagtcgtt aataattcaa cagcagataa agaacgcgtg gcataagtag ctgacatact
    17401 gggaattgca gacgtgactg caccagcaga agtcaaaata agagagactg acagcagaga
    17461 aaatttcatc atctttttat tcataagcat gttcctttca tttttgtaag cgcttatcaa
    17521 aaatatagta tgaagtggtt attctctcaa tagcggaatc acaggaagtt catgtcccaa
    17581 ccatagaaag tctttatgaa accaaaaata gctttgcata taatagcagt acagcgtgaa
    17641 ctaataagcg aaagaaaccg cttacaaaaa ggagggagtc aattggaact agctgaatta
    17701 gacatttttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac aaggaatgaa
    17761 tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc accgagggga
    17821 tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg aagttcccga
    17881 gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt tacaaattat
    17941 taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata aagaagctac
    18001 tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat taaaagaaga
    18061 aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac tttcttcttt
    18121 tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt ttcgcggcgg
    18181 aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt ttgatacata
    18241 cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg aattgactca
    18301 tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg agatgcttag
    18361 agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa cagcagctca
    18421 ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg atgcttcctt
    18481 tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc aagaaacgag
    18541 tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt ctttattcaa
    18601 attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac attaagaaga
    18661 aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct gtcttattat
    18721 atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt tgtattatca
    18781 ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga attcgtttcc
    18841 atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa taaacaagac
    18901 tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc atgaaaaaaa
    18961 caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc gcttatgcaa
    19021 tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa gaaaaagcag
    19081 aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat gtggctgtat
    19141 ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca gctggtatca
    19201 accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca atcatgcgcc
    19261 aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt gcttcaaacc
    19321 cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca tcacgtgtta
    19381 tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca ttgaaattaa
    19441 aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat tcagaagttg
    19501 ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta gaaaaagacc
    19561 atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat gctgcttatg
    19621 agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact gcacgtatcg
    19681 taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat ttaactggtg
    19741 aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa aatggtgttc
    19801 gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc aaatcaacaa
    19861 gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg aactaaaaat
    19921 cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat atctaatagg
    19981 taagcaaata aacagaatgg ttaaaattaa tcggtccgaa aatgtatccg tacatatgaa
    20041 catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt tatctgtggt
    20101 taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt cgttcatatt
    20161 tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa aaaagtaaga
    20221 aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa aatgacgatt
    20281 tttgataaaa aaccgttcga gagaggttta tca
//
LOCUS       DAXWLH010000043        20269 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXWLH010000043 DAXWLH010000000
VERSION     DAXWLH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.043"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1087
                     /locus_tag="ACGE36_001129"
     CDS             <1..1087
                     /locus_tag="ACGE36_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8383540.1"
                     /translation="TFMMMVTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLIN
                     GVLFNTFLIVFGGYLGWLLIETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGV
                     VVMKKESRSLANLLTLLLGVALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVF
                     FNFMTVSILYQFNRPRYNQDYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATL
                     NPPVLLMSGGKGTDEKVAEAIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMD
                     QQMNGPYRAIFTSNNYHIFRAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVL
                     HKKRHLIICGLMIGFAVFSALILKFAQ"
     gene            complement(1264..2532)
                     /locus_tag="ACGE36_001130"
     CDS             complement(1264..2532)
                     /locus_tag="ACGE36_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFF8383541.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(2583..2650)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2693..3340)
                     /locus_tag="ACGE36_001131"
     CDS             complement(2693..3340)
                     /locus_tag="ACGE36_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFF8383542.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3605..4108)
                     /locus_tag="ACGE36_001132"
     CDS             complement(3605..4108)
                     /locus_tag="ACGE36_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8383543.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4232..4996)
                     /locus_tag="ACGE36_001133"
     CDS             complement(4232..4996)
                     /locus_tag="ACGE36_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFF8383544.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5104..5379
                     /locus_tag="ACGE36_001134"
     CDS             5104..5379
                     /locus_tag="ACGE36_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFF8383545.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(5727..6878)
                     /locus_tag="ACGE36_001135"
     CDS             complement(5727..6878)
                     /locus_tag="ACGE36_001135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8383546.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(6898..7620)
                     /locus_tag="ACGE36_001136"
     CDS             complement(6898..7620)
                     /locus_tag="ACGE36_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8383547.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7613..9064)
                     /locus_tag="ACGE36_001137"
     CDS             complement(7613..9064)
                     /locus_tag="ACGE36_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8383548.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9206..10288
                     /locus_tag="ACGE36_001138"
     CDS             9206..10288
                     /locus_tag="ACGE36_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8383549.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10622..11560
                     /gene="yidC"
                     /locus_tag="ACGE36_001139"
     CDS             10622..11560
                     /gene="yidC"
                     /locus_tag="ACGE36_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFF8383550.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11741..12145)
                     /locus_tag="ACGE36_001140"
     CDS             complement(11741..12145)
                     /locus_tag="ACGE36_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8383551.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12161..12970)
                     /locus_tag="ACGE36_001141"
     CDS             complement(12161..12970)
                     /locus_tag="ACGE36_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8383552.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12957..13847)
                     /locus_tag="ACGE36_001142"
     CDS             complement(12957..13847)
                     /locus_tag="ACGE36_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8383553.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(13860..14339)
                     /locus_tag="ACGE36_001143"
     CDS             complement(13860..14339)
                     /locus_tag="ACGE36_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8383554.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14356..16122)
                     /locus_tag="ACGE36_001144"
     CDS             complement(14356..16122)
                     /locus_tag="ACGE36_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFF8383555.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFATGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16168..17109)
                     /locus_tag="ACGE36_001145"
     CDS             complement(16168..17109)
                     /locus_tag="ACGE36_001145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFF8383556.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17106..18101)
                     /gene="lacD"
                     /locus_tag="ACGE36_001146"
     CDS             complement(17106..18101)
                     /gene="lacD"
                     /locus_tag="ACGE36_001146"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8383557.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18094..19287)
                     /locus_tag="ACGE36_001147"
     CDS             complement(18094..19287)
                     /locus_tag="ACGE36_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8383558.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19305..20033)
                     /locus_tag="ACGE36_001148"
     CDS             complement(19305..20033)
                     /locus_tag="ACGE36_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8383559.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         5873 a         4392 c         3556 g         6448 t
ORIGIN      
        1 gacttttatg atgatggtca cttacttcgt cgttttagaa gttttttatc atacatttta
       61 ttttttgatt ccattgctat ttttcagtat ttttgcattt ttctatttta aggaaaaacg
      121 taggttaatc aatggcgtac tttttaatac gtttttgatt gtctttggtg gttatctagg
      181 gtggctacta atagagacgc aaaatatctt tatcggtggg ttattggctt tgatcgggat
      241 accaatatta attcttttgg caatcggtat ctacattttg atcatttttt tattttggaa
      301 tggtgttgta gtcatgaaaa aagaatcccg ttctttggcc aaccttttga ctctcttgtt
      361 aggagtcgct ttgactatct ttttgatatt ggactataca ttagtccatt ggctcccaca
      421 atgggcaaat gctctatttg ctgtcattcc gtttattttt gcttatctac tcatcgtttt
      481 ctttaatttt atgactgttt caattttgta tcagttcaac cgtccaaggt ataaccaaga
      541 ttatatcgtt gttctcggcg ctggcttgat tgatggtgaa cgcgtatctc ccctacttgc
      601 taaaaggatc aataaggcga tcgcatttta ttgggcacaa agtaaagcaa ctttgaatcc
      661 gccagtcttg ctcatgtcag gaggaaaagg aaccgatgaa aaagtagctg aagccattgc
      721 aatgaaacaa tatgctttag aaaaagggat tcccgagcgt gatattttag tagaaacgaa
      781 ttccacaacg acgctagaaa atatgctgtt ttcaaaagaa ataatggatc agcaaatgaa
      841 tgggccttat cgcgcgattt ttacttcaaa taattaccat atctttagag ctggactata
      901 tgcgcgacaa gcgaaactca atgcaaacgg aatcggtgca aaaactgcac tatactactt
      961 accgaatgct atcttaagag aatttattgc catccttgtc ttacacaaaa aacgccatct
     1021 tatcatctgt ggcttaatga ttggctttgc agtattttct gctcttattc tcaaatttgc
     1081 tcaataaaaa agagcgtggg acaaaactaa aaatcagttt tgccacagac tctaactccg
     1141 aataaacggt gggagcagaa gcaactcctt cggaaataag cgaaattcac aaaaatttaa
     1201 aaagcaattt tcgtgaattt cttcttattt ctcggagtta aacacttctg tcccaacctc
     1261 tttttatttc tctttttgtc gtgagattct tccaaggatc ggtaggaaca agattccttt
     1321 tttcacttca actttttgcg cagaatagat tccactattt cctgaacaca taaagctaat
     1381 caaacagatc ataaagaagt agctggctgc ttgacctcca aaaagttcga tccccataac
     1441 aaagcaagca atcggtgtat tcgttgctcc agagaacacc ccaataaacc ctaatcctgc
     1501 taaaaaaggt actgatacat gaagaatggg tgctaatgtc gcacctaaag ttgccccaat
     1561 ctcaaacaaa ggggtcactt cgcctccttg gtatcccgcg ccaagtgata gaacggtaaa
     1621 gccaagtttc ccaagaaaat cataaggatg agcacttcca ttaaatgcat cttgtaataa
     1681 aggtaagctt aatccgagat atctttgcgt ctgtaaaatc actactacga gtaccacgac
     1741 tgctcctcca ataaagttgc gtaaaactgg atgggggatc cgctttgcat accaagcttt
     1801 taaccagacg atcgaacggc taaagagcca gccaatgata ccaaatgcaa tgcccgcaag
     1861 caacaatttg aagaaaagtg aagggtccca tgcaggaatc gcccccattt gataatgggt
     1921 atgcttgaca ttgaaacttt ccgttacaaa gttcgcaaaa agtgctgcaa aaaagcttgg
     1981 aaacagtgct tcggttctca cttttccgat tgccaatact tctaatccaa acaatgtccc
     2041 agccaagggt gtcccaaata ctgagctaaa acctgcgctg atcccactga tgacaagaat
     2101 ctcacgctcg attttgttca attttagcca acgagcaaaa ttctcagcaa gtgccccgcc
     2161 catttggacc gctgtaccct cccgaccaac agaaccacca aaaagatgcg tggtaatggt
     2221 gccaaataag gtcagtggga tcagccgtaa tggaatcttt tcatttcctc cattcccttg
     2281 atcgatcact aaattgtttc cccgggaaga attgccccca tacgtcgtat atagataagc
     2341 aaaaagtccc ccacttatcg gcaaaagaaa aagtaaccaa ggatgctcca gccgaatagc
     2401 tgtcacatca gtcaaactag ttagaaagaa tgcggataaa cttcccataa aaatacctaa
     2461 aattgctgtg atcaataacc atttgatcat atagatgatc accttcaatg gatcaggtaa
     2521 tcgattttcc atctcatttc ctcctcaaaa aaacgcctac taaaaaaagg ggtagccccg
     2581 tttctttagt aggcgtcatt agttatctta cgatcaacgt ttaaaggcga acaccatcac
     2641 ctattcactt caccaaataa gatagcatat ttttcagaaa aatacaatga aatcaccgct
     2701 cacgtttcac tcgttctgtg aattcgtaaa aaggagctaa ttgtaccaac tgctgattac
     2761 attggtcact atcatgacaa atataatttc cttttttcgt gtatgttcca tctgaaccag
     2821 tttttgtggt agaaagaaag agtgaaacat ttgaagttct ttgacaaatc gaacaaattc
     2881 ctttgttgat tgtaggagat aatgttccac tgatcccttt tagtttttct tcttcataat
     2941 agatcataaa ttttttttgg ctacctggat cattccaacc aatataagta tgttccctta
     3001 aatccagttg attccaatct ggttgtttta actttttcac ttttcggaat aatttatcga
     3061 tctgcggttt tgtcggttgt atgaatggaa gaacgatcac tttcaattcc tctaatagtc
     3121 gttctgcttt ttcttttttt agagtgacgt ctagtacctt ttccataaat tcatttacag
     3181 ctgtattagg atgaccaagt acctcgtgga tcttttccgc agctaatgct tggactgttt
     3241 ggactgtctt tggatcattc actgaactat aggcgcttaa cagctgctcg acttctcgct
     3301 tgataaaata atattgatat ggtttgattg ttttttccat gactttgagt cctttcttct
     3361 ttcaaaatcg gctactttga agaaaaaagt gatctttcta tcaaagtgcc tttagataaa
     3421 gccctcaaac tcttttaaat aagatacaca tcgcatctta tcatcacccc ctttattttt
     3481 atgttggtta tgtacttttt catctcaaca aagtaaacac taaaaagacc aattagtcaa
     3541 tgtctaaaca aaaaacccga ggattttttt caaaatactc gggtcaaata cttagttaac
     3601 taccttaaat cgattgttct ttaccgaaat atcgttgcca tttttgtttc attgaagctg
     3661 tcattggttg agctgcttct ttgactgcac aatatttatc cacaaaagta acgatataac
     3721 cttctttata tttaggaggg gcaatcgttg ctccccacat atgtttcaaa ataatatcac
     3781 gctctagatc tgaaagctcc gtgatttttt cagcattttt cacagcgatc cttgggtgaa
     3841 catacgcatg tgtaccttca tcgaacttcg tcgtacgcca atcgtagtaa aataaatcat
     3901 gtaaaaggcc tgctcgagca gtcgctcttg catcaccgcc ccattttttc gctaatttgt
     3961 agctatagta agacacactg attgaatgct ctaatcgtgt cgagtggaca tgttgcgtgt
     4021 aatttgctaa tttctttact gcatcagttt ctaatagatc atttatataa gacatatatt
     4081 cttcgtcttc acgccatttt tctgtcataa ttatcgatct gccacctttt tgaattccat
     4141 ttatctttga aattgaactt agtataccac tagtgttcgt aatattctag tgaggttttc
     4201 gcaaaatgca gaaagcttaa tagttcctta actatacgga gaagtgataa agcatgatgc
     4261 ccgctgcaac tcctacattt aaagactccg catttccttg aatcggtata tacacatttt
     4321 tatccgttag cgccagcact tctggacgta cgccttgacc ttcattcccc atcacgatcg
     4381 cataattttc tgttgtctgg atatcattca gggacactgc ttcttcattc agttctgttc
     4441 cataggttat aaatccttgc tctttaagga taggcaatag ttttgttaga tcctcttgga
     4501 tgatcggtaa atgatagtta ctcccctgca tgcttcttaa tactttggta ctgtagacat
     4561 ccgccgttcc cttacctagt acaacacaag atagaccaaa agcatcggct gtgcggatca
     4621 ttgtccctac attgccggga tcttgtacgt tgtcgagcaa caaccaacca ccttcaaaat
     4681 tcggtatctc tcttttttca gcttccacca ctgccatgat cccttgcgga gttggtagat
     4741 ctgaaacagt tgccatcact tcttctgata ctagtgtaac agaaagcgcc tgattttctt
     4801 caagccaact agcccactca gtcagaccgc gttgcgtaat aaaaagttcg ttgaccttcg
     4861 catttgaggc aacagcctcc tcgatcaggt gaaacccttc aagcagatat tccccttttt
     4921 cttcacgata ttttttctta tgtaattttt ttaattcttt gattcttgga ttttttgctg
     4981 attggatctc tttcacattt ttcacctcaa ctgattatac catggattag atcattgcac
     5041 ttgttcagta gcaaaaaagt cggtatcatt aatttaaaaa taaaagaaag aacggtgaat
     5101 aaaatgaaca aaatcaagat gaacgtgcaa ggacgtgtac aaagcgttgg ttttcgctat
     5161 atgacaaaaa tcgtagcgga tcaattaggg atcaccggaa cagtgaaaaa tgaagatgac
     5221 ggctctgtca cgatcgaagc tgcgggcgac gatgaggcaa tcaaaaaatt cgtggaagaa
     5281 gtcaaacgat caccaagccc cagcggacgt gtccaatatg tagaccttca ggaaaatccg
     5341 atgatggagg acagaaaaaa gtttgttgta ataggctgac cttctagaaa tgaataggaa
     5401 atgtcagaaa aatcggactg tctgtatgta gatcgaaaaa gtttgcccct ctctccatcc
     5461 tagatcgaca aaaaaacatt cttattagcg agtttagtaa ccagtgatag gcatacgtaa
     5521 aagcgggagg acttcaaaaa ttttggttca ataaaatgga gtctataata ttttatggta
     5581 gtggaaaatc gactttgatt ttccactacc actttttcat tgtcaaatcc tgacgctgcc
     5641 cagtcaacaa gccaactctc gaaaggatca ttttccctct ttgttctaat ggatacccat
     5701 agctgttttc aaatatacag ttttatctaa aaatttagtt ttgctaaacg atcaaaggct
     5761 tcacctaaga tttccaatgg ctgagtcgct gctagtcgaa tatagttgtc cccgatcgtt
     5821 tccccaaacg cttttccagg gacaaaaagt acgccgcttt tttctaaaac ctcttccaca
     5881 aacgttactg aatcgagccc actcttactg atattaataa aagcatacat gcttccagaa
     5941 acagaagata aagataagta agggatttct gccactcttt tctcaatata agctaatcgt
     6001 tctttgaata cttcaacaac tggcggtaca agctgttcat agtgctttaa ggcgtagatt
     6061 gcacctcttt gagaagcagt cggtgcggaa taagtgatgt tttcattgat caacccagca
     6121 atttgattga tgtatgctgg tgcgatcatg taccctaagc gccagccagt catcgcaaac
     6181 cccttcgata caccactgac agtgatcgta ttatcgggcg caaatcgcgc catcggtacg
     6241 aacttctctt taaaggcaaa gtcttcataa atctcatcgg aaatgatata gagttcattt
     6301 tcgattgcga cttcagcgat ttttttcaac gtgctttcag aaaaaacagc tcccgtagga
     6361 ttattcggcg aattgatgac gatcaccttt gtacgatccg taatcgctgc ttttaaatct
     6421 tccacatcca actggaagcc cttttcttca taggtaggaa caaaaacagg cttgcctcca
     6481 gataaaatga cttgttcccg atatggtgaa aaataaggtt catggatgat gacctcatcc
     6541 tctggatcta gtaatgcctg taaaactaga tacatcccgt gcattgctcc gactgtagct
     6601 cgaacttggt tgcattcaac gctcaatcca tatttctttt gataaaaatc tacgaccgtt
     6661 gacaagaatt ctttgctgcc atcaggcgca gtataattag tagcaccatt ttttacatct
     6721 gtataagctg cttcaataat cgtcggatca gtgatcaagt caggatcacc aatcgataaa
     6781 tcgataatat cctctttttc ctttgccaaa atagcaatat tcatcaacag attttctgct
     6841 ggttgctgaa accggcgtgc aatcttactt ttgtccatct tacttcctcc atctttgtta
     6901 caacactttg ttcaagaaat cctgtgttcg actattttgc ggattttcaa aaatctcttt
     6961 cggtgttccc tcttcttgaa tgatgccgtc agccataaaa atcacacgat cggcaacttc
     7021 tttagcaaaa cccatttcat gagtgaccac gaccatcgtc atcccttttt tagctaaatc
     7081 ctgcatgact gccaacactt caccgaccat ttcaggatca agtgcagatg taggttcatc
     7141 gaaaagcatc acatcaggat tcattgctaa tgctcgagca attgctaccc gttgttgctg
     7201 tcccccagat aaactggtag gataactgtc cactttttcc tttagaccaa cttggttcaa
     7261 taatgataga gcatgttctt ttgctttttt agggtcttct tttttgattt ttacaggtgt
     7321 gatggtcaaa ttttcgagta cattcatatg gggaaacaaa ttaaaacttt gaaataccat
     7381 tcccattttt tgacgaattt gattgatatc gctgtcaggg gctgtaatgt tttgtccctt
     7441 aaaaaagatt tcaccagaag ttggttcttc cagcagattc atacaacgta gaattgtact
     7501 tttcccactt cctgaagggc caatgatcac cactacttct ccttctttca cagaaagatc
     7561 aattgatttt aagacttcat tctcgccaaa actttttgac agttttttta cattaagcat
     7621 tcagacccat tctcctttca actaaagcta atatacggga aatactgaag gtcaacacga
     7681 aataaatcaa agagaccaac acatatggta ggaatggttt gaagctagca ccttgtacca
     7741 cgcccgtttg aaagatcaat tccgataccc caatgaccga aacaactgag gactctttga
     7801 tgatcgtgac aaactcattt cctagagctg gaagaatatt tttgattgct tgtgggaaga
     7861 tgatgaaacg catggcttct ttctggtcca ttcctaaaga acgtgcagct tccatctgtc
     7921 ctttgttgac cgcattgatc ccggcacgga caatttcggc tacataagca gcactattca
     7981 acgagacagc aatacaacct gctaccagtt tcgatagatc caatcccagt actcctgttc
     8041 cgaagtaaac gataaagatt tgaactaaga gaggtgttcc ccgtacgtat tcgatataaa
     8101 taatggctaa tgcctttaaa aatttattct tacccaattt catcagtgca agcaaactac
     8161 caaagaccat tccaaacaat acacccacaa aagctaataa aatagtgtat tttgttccaa
     8221 tcaaataata cggaccgtat ttttgaagaa aggactgatc atcttggaac atcaattgat
     8281 tcgcttgttt ttgataatct ttcagcaagc cgtcgtcatt gatcgttttg attgacgcat
     8341 tgacatgatt tttcagttct ggtgtatttt ttgacatcgc aatagctgtg gttttttcgc
     8401 cttgcgcaaa ttttactgga gtcaaagcta ctttcttatt ctgttgggca tatgcttctg
     8461 caactggttg ttccagcaca acaccttcaa ctttttcatt cagcaaatta ttgatcagat
     8521 caggaacttt ttgcagggag gtcaactctg caccagtcag ttcatttttc actaactcct
     8581 cttgtgtggt ttgcttttgc gccccaacgg ctaagccaga aaagtcggct gtcgtcttaa
     8641 atttattttt gtcctcttta cgaacgagta ctttttgctg taccacgaaa tatgggtcag
     8701 aaaagtcaac ttcttgtaaa cgttcttcag tcaccgccat accagatata actaaatcga
     8761 tcttgccagt tttcaacgca cctaataatg catcaaaacc aagttcttct acttttagtt
     8821 tcacacccat atcctttgcg atcttttcgg caatggatac atcaaaacca acgattttat
     8881 ccttaccatc cacattcgca tggaattcat agggcgcata gtctgcagat aggccaacga
     8941 ctaattctcc actttgtttt atccgctcgt aaatttcatc tttttctgca ccttgtactt
     9001 ttactggtaa aaaaaccagt agaaacatca gcgccactaa taatatgttt attccttttt
     9061 tcatccattt ggccaccttt gaataataat gtaatcagaa aagctataaa agaataaata
     9121 ttcataaata acttgctaat atactagcat aactattata ataaaaaaag attaatttaa
     9181 aaaaataatg gaagaagttt tttatatgac aaaaaattta attgttcgcg gtgcccctca
     9241 agagtatgaa ataaaagaaa acgcatggga ttcactccct gaacaccttt acaaaaggca
     9301 tattgacaac gttttgattc tccatggaaa aacatcttgg aaagcagtaa aagatgtttt
     9361 tccagattta tcaataacaa aaatttttga atattatggt ggagaatgta ccgatccact
     9421 tgctgacagt tttgtttctt tatgcaaaaa gaaggatttc aatggtatta ttgcagtcgg
     9481 cggcgggaaa gtagcagatc taggaaaatt gattgcctat aagacaaact taccaatcat
     9541 catcttacct accttagctg cgacgtgcgc agcttatact ccacttagtg tggtctataa
     9601 tgaagaagga gtgatgcttc gctatgatat gttccctaaa gcaaatgatt tagtgttgat
     9661 cgatccaaag atcattttgc actcccctcg cgagatgatg attgccggta ttggtgacac
     9721 cttagcaaaa tggtatgaat ctcacgcaat catctctcag ttaactatca agcctatcga
     9781 agtccaagta gcggaatttg cagcgttacg atgccgcgaa atccttttag agaaaagtgc
     9841 agaggcaatg gaagcgatgg accagcaaaa aatcaatcaa gcttttatcg atgtcgtgga
     9901 aacaaacatt ttattggctg gtatggttgg tgggtttggc gatgactacg gccgaacagc
     9961 tggagctcat tccattcacg atgcgatgac cgtactccct gatagccatc agcaacttca
    10021 tggaaacaaa gttgcatacg gtatccttgt ccagctgatg atcgaaaata aattagcaga
    10081 aatcgaacac ctactgccgt tttatcatca cttgaactta ccaacgagtt tagctgatat
    10141 gggtttacat ttaacagaaa gtgactatca agccgtagct gaacgtgcat gtttacctga
    10201 tgaaatgatc cacttcatga aagaaacgat cacaccagca ctggttgtcc agaaaatgaa
    10261 agagttagaa caagtgacac agggatagtt agccccttat accatggcag tcagcttttt
    10321 tatggatcgg ctcaatggta aaaaaaaaga agtggaaaaa attgacattc cacttctttt
    10381 ttttgcgcta aaattatgat ttcaaggaaa tatccgttgc tcatcttatt ttagagaacg
    10441 gaggtgctaa gtatgcttta tactgaagta tttttagctg gattcttctt tttaacatta
    10501 agcgtctttt ttgatcctcc aatcggttat ttctatgaaa tttctatgaa gttgattgaa
    10561 ttatgagcgc ttttttagta tagttaagct tgtgtaaaaa ttataaaaga ggaagtattt
    10621 tatgaataaa tggaaaaatt ggttattggg ctctgggctt gtcacgatca tgcttttctt
    10681 gtccggttgt gtccgcatgg attcaaatgg taaccctgac acttcaggaa tcatttaccg
    10741 tatattggtt cacccaatgg gacaagcgat tacatattta gtggaaaact ttaattggtc
    10801 ctacggctgg gctgttatct tgatgacagt catcgttcgt atcatcattt tgccattagg
    10861 tattagtcaa tcgaaaaaaa caatgatcca atctgaaaag atgcaagcat tgaaaccaca
    10921 agttgaagct acgcaacaaa aattgaaagc tgcgacaact cgagaagaac aaatggctgc
    10981 acaagctgaa atgcaacaag tttatcgtga aaatggctta agtatgactg gtggtatcgg
    11041 ttgtttaccg cttctgatcc aaatgccgat cttttctgcg ctatatttca ctgctcgcta
    11101 tacagaaggc atccgtgaat caacctttta cgggatcgac ctaggtaacc ctagcttagt
    11161 tttagtagcg attgccggta ttgcttattt acttcaaggc tatatctcaa ctatcggtat
    11221 ccctgaagaa caaaagaaaa cgatgcgtac gatgttgatc gtatcacccg ctatgatcgt
    11281 cttcatgtcg atcagtgccc cagctggtgt cactctttac tgggtagtcg gtgggatctt
    11341 tagttgtcta caaacattca ttacgaatgt catcatgaaa ccaagattaa aagcgcaagt
    11401 tgcggaagaa ttaaaacaaa atccgccaaa acaagtggtg acgcctcgta aagatgtcac
    11461 acctgctgaa cctgcgaagc aaacaaaaaa acaagtacaa gcaccaaaaa acaatgcgaa
    11521 gaatggtcgg aatgctggta aacagcaaaa gcgtaaatag ttatttgtaa ctagcttctt
    11581 cgatcgaccc agatcatttc tgattttatg acgataaaag ccaacgtact gtgaaatata
    11641 gaaaactata tttcgcggta cgttggcttt tttatacacg gcctctttat acatccgtat
    11701 ttatatccag ttgtatcacc agtcaagaag ttgtgcttga ttattcttct tcatcgtctc
    11761 ccatgccagc aatcacttgg ttcactgaaa cgatactctc acgacccgaa ccaataaaat
    11821 ctggttctgc tccaatcatt tgcgaattga tgaattcgat gaccatcggc aaattcatgc
    11881 cagcatatag ttcgatcgtt tcaccttcca tgattagctt ggaaacggta tttgcaggtg
    11941 tcccacccaa cagatcgcaa agcactacgt aatctttcaa tggtgctgta acggagagga
    12001 atctttcttt atacgtgtcc gcgccatctt caggcaataa agggatcgta tagatatttt
    12061 cttgtggtcc catgatcatt tctgtactgt tttttagttc ttcacaaaat ttaccatgac
    12121 taatcaagac tagttgtcta ctcatctttc caattctccc ttatttcgaa atcacaccta
    12181 atgcagataa tgctacacat acgatcaata cgatgaatat tgctcgagta ggtgtcatat
    12241 ttttcttacc taacatccaa taaacgccac caacgattcc agctggtaaa agtttaggta
    12301 agatcatatc gatatagttt tgcatattca acgttacatc cccaatcgtt ggtgcccatg
    12361 agaatttaac attgatcatg gttgctacta acgctccaac catgaaaact cctagtaaag
    12421 tagccgcatc tgtcaatgca gtcaagcgtg attgcatcgt ggtaactaga gaaatccctt
    12481 ctttgtaggc aaattctagt tgtttccaac gaaagaccat gacagcgacc tgagcaagga
    12541 tccagataag gatacctact ggattaccat ctgttgccat atttgcggct aaagctccaa
    12601 atatggtcgg gatcaatgcc gcaaatacag aatcaccaat tgcagcgaag gaacccatca
    12661 atcctgcttt catcccagaa acagtttcct ttgaagagac tccttctttt tcttcgatag
    12721 caagatcgat cccagtaatg atcgtgttaa agaaattaga cgtattaaaa aattgcgtat
    12781 gtgttttcat catttctttt aattctggtg tatcatcacc atagattttt ctaagttgtg
    12841 gcaagatagt atacagataa cctgaacctt gcattctttc atagttccaa cctaattgga
    12901 aagtaaacaa acttctacga ttgatctgct taaaatcttc tttggtcaat ttataattag
    12961 atttcgtcat cttcgatttc cccctcatca gattgtgatt gctgacttac tactggatgt
    13021 tggtcattct tttgatcatt cttataactg atcgttgcca gtgctagacc aatcaatgcc
    13081 acagcaagca tcggtaatga attgaatgtt cctgggaaat ctttgaccac gctagccaca
    13141 cttgttccta acaattggac attcgagaac actgttgcta ataacgctgt gatcgtaaat
    13201 cctaaaatca gataagaaat gtgtttcttt actggtaggt agcgtagtaa aattgcaaac
    13261 ccaactgctg gtaagactgc ccctgcaaca gataagccat ctcctagcca ttttaggtcg
    13321 ccgtttaaaa cagccacaac attttggact aagccaccac cgaatgctag ggccaagaat
    13381 acaggaacca ttctggaaag tgcccaaggt aatgccccca tcaagaagtt ccgttcaatt
    13441 gctttgtaat tcattgtttc cacatgttga tcgatccgat gtgcaaaata cgtattcgca
    13501 aaacgtgcca aaatatctaa ttggatcatc aaacttgcta caggaaccgc gatggccgca
    13561 atcgcttgtt ctgggttcat ccctagagaa atggagaaag ctgttgctaa gatcgttcca
    13621 gagttggcat cgatttttga tgcaccacca aatgtcccta cccctaaaac agtcaactgc
    13681 atacttccac caatgattaa acctgctttt aaatcaccca ttactaaccc agcatataat
    13741 cctgcaaaaa ctggggcgct cattgaagaa taaatctgta attcatctaa aatttgatac
    13801 cctgcatata atgttaataa taatatctgc caccattgaa tatccatgaa tcattcctcc
    13861 tatttgatta aaagactcat aaagtcttct gctttgtcat ttggcaccat ttgagcgatc
    13921 aaatgaacgc caagctgatc taatttcttg aagacctcta catccgcatc tacgacattg
    13981 attgattttg tgatggaacg tgtttcatct gtttgtgaca tattgccgac atttaattct
    14041 ttgatcggta cacctttttc tactagcgct aagatacgat ctggtttttt cgctacgatc
    14101 agcactcgtt gagaatcata tttacctgct aaaatatttg cggctgcttt ttcaataggt
    14161 aaaatactta atttgactcc agctggcgtt gccaacttta acccactttt ttcgatcgca
    14221 tttgttgcta cctcatcgtc tacgaccatg atccgaccaa tattcaactt tgtcgtccaa
    14281 agatttgcca cttgtccatg tatcaaacgt ccatcaattc ttactccaat aattgccatc
    14341 atccattctc cttttctaac ttttttctat tggttctttg acaaaatcta taacttcact
    14401 gaatctacct tctgtttcaa agataacgat ttcattttgt ccttttctaa ataaaccctc
    14461 gggaacaaac agtgaaagaa ttggtcctac ttcccaaaaa cgaccaatat tcgtgccatt
    14521 cacccaaacg atccctttcc caaactcact aaggtccaaa taagtatcac cgatttctga
    14581 cagttccgct tcaaagcggt aaaatgctgg ttgacctgct tcccattttt ttgaaaaatc
    14641 gatgtgttct gtagtctcta aaggtaaaca atactgtgtc cattctgtga taaaatgaag
    14701 atcgctcatc actcctgtac gtattccttt tctttgcgta tcagctagca gcttgtgtcc
    14761 atagttgacc cgtcccatat tttcaaataa tagatcaatt tgatggtctt ctttctcaag
    14821 tgtcaatgga atggtctcac cgatctctgt ttggtattga atcgcttgct tttcttcatc
    14881 gagaaaaaca tggacccgat cacgtccgtc gatgatccgt aatttttcat tgaccgcatc
    14941 ttttttgatc gttgttcgat acaataggta cccagtattc tgacccaatt cttccattgt
    15001 ttgaggatag gtcgagtgga tcggcttgct gatggtatcc agtgttgcga aaaagctgac
    15061 tttttctttc aagttgattc cttttttgac gattgtttct tttatccatg gttccatttg
    15121 ctgcatttct ggaaaatttt ctgccatcat tcgtttcaga gcaaaatatt tttccgttgg
    15181 atttccctgc tcatccaatg gtgcatcgta atcataggaa gtgacttgcg gcaaatcaat
    15241 cgcgcctctg gctgaacagc cattcataaa cccaaaattg gttccgccat ggaacatata
    15301 gagattgata ctgccgatct gtaatgcttc tttgactgca tccgctaatt cttccggatc
    15361 tcttttaatg atcggctctt tccaacgatt aaaccagcca tcccaaaatt ccatacacat
    15421 caacggccat tttttaccat gtgcttcaaa aaactgggcc atatctgcaa aattttcttt
    15481 tgcttttgag ccaaaattcc ctgtcgcaaa aacatcatcg tcaatcaaaa tgcctgctct
    15541 taatgtggct tgccaaggtc catcagaagt gaatagcggt acatcgattc caccttcctc
    15601 caagtatttt tttacttggc gcaggtatgc tttctcttca ccatacgaac cgtactcatt
    15661 ttctacctgc accattaaga cgttgccacc atgagtgact agatgaggaa cgattttagg
    15721 tagtagcgtt tggtaatacc gtttgacata cttcatgaag cgaggatccg ctgaacgaat
    15781 acggcactcc tttgtcaata gccaagcagg caagccacca aattcccatt ctgcacaaat
    15841 ataaggggct ggacgtacga ttgcgtataa tccaagggat tgcgctaact ctagaaaatg
    15901 ggaaagatcc aatattttcg aaaaatcata ggttccttct tctttttcat ggagattcca
    15961 aggcacatag gtttccacag tattaaatcc aagcgctttc aaattgtgca atgaccgctc
    16021 ccaattcgtc ggatgcactc ggaaataatg gatggcacca gataaaattt tgaaaggttg
    16081 accgtctaac aaaaactctt cttttatttc aaatctagtc aagcaaattt cctcctcgtt
    16141 tttagaaacc gctttctaat tttataatca acgaatcttt ctgacgttga tttcccccct
    16201 aaaataatcg actttcacag gatcgatcca tcctgttttt tcttgttgtg cattcgccat
    16261 cccacaggcc atcgcaaacg caagtgtttc ttcaatcgta tagctctttg atagagcatg
    16321 tgctaagcct gcaatagtcg aatctcctga ccccacagga ttcaccaccg cgattttagg
    16381 aatcgtaacc tgatagaagt gtctccctac tttagcgatt gctccttttg cgcctagcga
    16441 aagaacgatc caattgattc cttcaaaaag cggatgatcc aataatgatt ggatttcatc
    16501 taacgaagta gccgaagaaa ttgtttggtg gaccaactct cctaattctt ccaaattagg
    16561 tttgatcaaa aatggttttt ccttgccttc caagacttca agcaaggttt gaccagaagt
    16621 atctaacaat actcgtgttc cttttttatt cgttagggca agtaactctg cataataact
    16681 tttagaaaat ccttgaggca gactacccga aatcgtaaag atatcagttg tttccattag
    16741 tcgtgcaaaa tgctcggtaa atttatgttg ctcttcttcc gtcacaacag gaccgctttc
    16801 caatagttct gtttgattca ctccatgtaa aatagcaatc gaatctctcg tttccccttt
    16861 gataggtaaa aattgatgcg ttatccctga ttgatcgagt tgttctttga tatatgctcc
    16921 atgctgcccg ccgactaatc cagtagctaa aacatcatct gctaataaat gaaggacacg
    16981 agcaacattc aagccttttc ctccagccgt tttgttgacc gttttgaccc gattgacttg
    17041 gtcgatccgt aattcatcca acgtgtaagc gatgtcaatt gaaggattca tggtaactgt
    17101 cgtgatcatt ttgctgtcac cgaatcttct tttactttgt caaaccaagg ggtggcgttt
    17161 tcatttatca ctttattcaa cgattcgata ttttgtcgcc cttgtgttct caaccactca
    17221 atcgcacctt ctctaccatt ttcaacatat gctttgactc catctttcca agtcgcacgt
    17281 ccgcataaca caccgttgaa tgttgaacca gattcatgag caaagatcaa tgtttgttgg
    17341 aacaatgcag tcgaaacccc tgcactcaag aagataaatg gtaacggtgt tgcatcactt
    17401 tgttccttga agaatgccgc tgcttctttt ttactatacg caatttcacc atcagaataa
    17461 ccttctacat agttcatatt gactggcact tctactttca atacatccac tttatattta
    17521 ggatcagaaa attctttcat catctcgatc actttatgcg gtttgatctt cgcatattct
    17581 aatgaagtgc tgtcattggt tttggcatca tacgaaacca attctagata aaacggacgc
    17641 ttttcacctg cacattcact gcctaaacgt tcgataaata catgtttcaa atgattgatc
    17701 tctgggtctt catctacatc ataataaagc aaaaacttga tcgcatcggc tgtttgttcc
    17761 tttaatctaa gcgtagacca atccgctaat aaatcaggca gacgtcctgg agttgtcgca
    17821 tcataacccg ttttttcata ggctaaaagt agtcctgctt cctgatgtct ggctttagca
    17881 gctggcaatc catactctgg atctagtaaa atagcagaag cataaggcgt taattctgct
    17941 gatactaatt ctttgaattc agtgatctgt tgcgccgttg gttcttttcc taacgctttg
    18001 atcatctttt tcaaagcacc gcgttgatca attgccaatg cactgatgat tccttctttt
    18061 gttgataaac tcttcatagc tgcaattttt tcttcattca tccttcgttt cccctttgtt
    18121 cacatattca tggataatga cacctttgac cactcgattg actgtacctg taggcgatgg
    18181 tgtatctggt ttattcgcta cttttatcga cgtatcgagc gcaatgattt gggctgtcat
    18241 gatcataggt aaggcaagat acgcatcagg taggctgatc gaagcttcta acaaaagact
    18301 tgtacctgtg aaatcgatgt ttcctggttg ggcgatcgcg atgacactcg tggtaatttg
    18361 gtcatctttt atttcttcta gaatatctaa gtcataattt cttgtatacg gttggttatt
    18421 aacaaaacca ataacaagtg tttcgctatt taaaaacgat tttggtccat ggcggaagcc
    18481 catagaagaa tcaaagatcg ttgccacttt accggctgtc aattccaata ttttcaattg
    18541 tgcttctctc gttgcaccag ccaaacttcc tgaaccaaga taggcgatac gttcaaatga
    18601 cttaggcaat aatttttgga tttcttgatc tcgtgaaatt agatcttctc cagttctgat
    18661 caattgttga agataacttt cttttttctc aacggcagtt tggtcgaata cccataacgc
    18721 actcaacaac atgcacgtaa aacttcctgt catggcaaat cctgcatcat ttgatttttc
    18781 tggcatcagt aaaagataat ttcgttcatc tgtcgctgca ttctttgcta actgaccttc
    18841 tttagcacaa gtaatcgtca aatgaaacaa tgtcttgatt tgttcattgg caatcttgac
    18901 tgttgccaca ctttctggac tatttccact acgtgcaaat gacaccagta atgtcggttc
    18961 atccggaaat agaaattctt ttggtgaaga cacgatatct gtcgtaccaa agctatgaaa
    19021 aataaagtga tcgttatcac cgtattcatt taaataaggt actgctgtat ccccgacata
    19081 ttgtgatgtg cctgcaccag taaaaatcac tcgaactctt ttgccatttg ctgctttttt
    19141 tatctggtca aaaaacaatg ctaatttttc ttgattctgt gctaatatct cagctgtttc
    19201 tttccataat tgcggttgct gtttgatttc tcgcgtagtt atttccgcac ccaactcttt
    19261 taatttatcc ttcgatagtt caaacatgtt gttttccctc tcaatcattt tcggacgtgg
    19321 cggatcttat aatgaaactg gtctgctctc gctacactat ccgtatattc aataatttca
    19381 tttttcacat tataggtcgt tcggcttaga tataataccg gggcaccatc agcgatacga
    19441 agcggatcag catctttctt ttgaacgata cttgcataaa attcttcatc tgctaattgg
    19501 acttgctgtt cataatcctg agaaaacaca tcatacaatg gcttactttc aagtaatggc
    19561 agatcaagac caataaatag ttttgcaggt aaataagaac gttccaccat caatggttcg
    19621 tcatccgcta aacgaagtct tttcaatttg aaaactgttt cgcctaaatt gatgtttaga
    19681 ttcttggcaa cctttttgtt tgcttccatt tgcttgaatt ccaagatgac tgtattaggc
    19741 acccgtccca attccctcat ctcttctgta aaactatacg ctcccgccag atcagacgac
    19801 ttatctgata gatcagacac atatgttcct tttccgtgtc ggcgataaat atacccttgc
    19861 gtctctaatt cttgcaaagc aaggcgaacc gttgtccgac tcactccata atgctgacaa
    19921 agttctcttt cagataacaa tttgtcatga ggtgtcatct gtgtttcgat acgttctttc
    19981 aatacgtcaa ctagttgata atataatgat tttcctttac ttgagctctc cataaaaata
    20041 cctcttcttt actggttata accacaacac aagaataccc acatattcct catttgtcaa
    20101 agggtttttg gcgtaaaaaa gctattataa caaggtttca aggtaattta gaactggtat
    20161 caaaagatac ctcaaaaata ccagttgaca aataaaaaac aaaaaaaagc tgaatcttgg
    20221 caaagattca gctaaatggt ctagagaagt aaagaaaaaa tattcttta
//
LOCUS       DAXWLH010000044        20259 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXWLH010000044 DAXWLH010000000
VERSION     DAXWLH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.044"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="ACGE36_001149"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="ACGE36_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFF8383560.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACGE36_001150"
     CDS             complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACGE36_001150"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFF8383561.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACGE36_001151"
     CDS             complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACGE36_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFF8383562.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACGE36_001152"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACGE36_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFF8383563.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="ACGE36_001153"
     CDS             3621..4100
                     /locus_tag="ACGE36_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8383564.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="ACGE36_001154"
                     /pseudo
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="ACGE36_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5401..6186
                     /gene="phnX"
                     /locus_tag="ACGE36_001155"
     CDS             5401..6186
                     /gene="phnX"
                     /locus_tag="ACGE36_001155"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFF8383565.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACGE36_001156"
     CDS             complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACGE36_001156"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFF8383566.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8645..9592)
                     /gene="arcC"
                     /locus_tag="ACGE36_001157"
     CDS             complement(8645..9592)
                     /gene="arcC"
                     /locus_tag="ACGE36_001157"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8383567.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9707..10726)
                     /gene="argF"
                     /locus_tag="ACGE36_001158"
     CDS             complement(9707..10726)
                     /gene="argF"
                     /locus_tag="ACGE36_001158"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8383568.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10817..12046)
                     /gene="arcA"
                     /locus_tag="ACGE36_001159"
     CDS             complement(10817..12046)
                     /gene="arcA"
                     /locus_tag="ACGE36_001159"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFF8383569.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12507..13208
                     /locus_tag="ACGE36_001160"
     CDS             12507..13208
                     /locus_tag="ACGE36_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8383570.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13780..14796)
                     /gene="tsaD"
                     /locus_tag="ACGE36_001161"
     CDS             complement(13780..14796)
                     /gene="tsaD"
                     /locus_tag="ACGE36_001161"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFF8383571.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14793..15257)
                     /gene="rimI"
                     /locus_tag="ACGE36_001162"
     CDS             complement(14793..15257)
                     /gene="rimI"
                     /locus_tag="ACGE36_001162"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8383572.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15264..15806)
                     /gene="rimI"
                     /locus_tag="ACGE36_001163"
     CDS             complement(15264..15806)
                     /gene="rimI"
                     /locus_tag="ACGE36_001163"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8383573.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15790..16512)
                     /gene="tsaB"
                     /locus_tag="ACGE36_001164"
     CDS             complement(15790..16512)
                     /gene="tsaB"
                     /locus_tag="ACGE36_001164"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFF8383574.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16703..17683)
                     /locus_tag="ACGE36_001165"
     CDS             complement(16703..17683)
                     /locus_tag="ACGE36_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8383575.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17707..19191)
                     /gene="galT"
                     /locus_tag="ACGE36_001166"
     CDS             complement(17707..19191)
                     /gene="galT"
                     /locus_tag="ACGE36_001166"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFF8383576.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19203..20192)
                     /gene="galE"
                     /locus_tag="ACGE36_001167"
     CDS             complement(19203..20192)
                     /gene="galE"
                     /locus_tag="ACGE36_001167"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFF8383577.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         5625 a         4248 c         3594 g         6792 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttaatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgctcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccaccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg catccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt aaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaattt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aactcctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagcg
     4501 gctcctttgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat ggacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcgtaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccctct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaaaagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgattcg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaacta gaaaagcgaa aaaatgtgag tcgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtact acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aacggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgcgaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgactggat ttactgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaggg
     5821 tattcaccag acttcatggc aacttctgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc tggcgctgat
     6121 atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga caagaatcca
     6181 gaatgacttt tatacaaaat cagaggtcgt gaaaaagctg tactgcatca agtaataaga
     6241 acagccaaac aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg
     6301 caagcttttg aacaccgttt attcgaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttacaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg cgcatatcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctcttcatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga catataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgata ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgtt cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgctcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttctgataa aaaaacaatc cagatggcct
     8581 aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa
     8641 caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt
     8701 ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgcct
     8761 ctactttcgg aagcatgctt cctggagcaa attgcttttc atcaatgtat tgtttcattt
     8821 ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat
     8881 tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg
     8941 aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa
     9001 caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga
     9061 tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac
     9121 gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt
     9181 cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat
     9241 ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta
     9301 cttcgcccat ggcattttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg
     9361 tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt
     9421 taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt
     9481 tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg
     9541 tagacaagat tgcattaccc cctaaagcga caactacttt tcgtttggcc atttcattcg
     9601 tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt
     9661 tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga
     9721 taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg
     9781 cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag
     9841 tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg
     9901 gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat
     9961 tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg
    10021 atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg
    10081 taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt
    10141 cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg
    10201 tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac
    10261 cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat
    10321 gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta
    10381 ccattttttg actgaatcca cggaattcga ttccatcaaa catacgacct agtactttag
    10441 ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct agatattcag
    10501 gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt
    10561 tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt
    10621 tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt
    10681 ctttttctgc taataagctt cttccttgga atactgattc tttcatatta aacattctcc
    10741 tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagacc
    10801 gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg
    10861 gaccaccacg gcctcgtgac aattcacttg aattgatttc taacactttg atgccatagc
    10921 tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga
    10981 ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat
    11041 taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta
    11101 agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca
    11161 tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg
    11221 taaatacggt atccaaatgc atcattgcac ggttgtttgg aattttgatt gctaacactt
    11281 tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat
    11341 tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc
    11401 cttcgatatg atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc
    11461 ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag
    11521 tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca
    11581 agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcgtatagac
    11641 tgctagaacg tacttcaata tcttttgtac gaaccccagc catgatttta tctaccattt
    11701 cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa
    11761 tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg
    11821 cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtctttg
    11881 caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac
    11941 gatccataat atctggtgtc aaattttcca cttcttgtcc aggccttttc agtaaaactg
    12001 tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc
    12061 ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct
    12121 cattcaaaat gagacattcc tcacattaat attcgataat taaataatta aacgtttttc
    12181 tatttttaag cgtaatttat tcatttttta ttctgtatac actttcaaaa aaatatttat
    12241 tcattgaaaa aaattttttg aataaattaa aaaaataaat tatattagcg ttgttagcgt
    12301 ttactatatg tattattgtt gttttttata cacaaatcat tcgcttcaaa acaaattatt
    12361 caataccatt gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg
    12421 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
    12481 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
    12541 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
    12601 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
    12661 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
    12721 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
    12781 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
    12841 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
    12901 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
    12961 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
    13021 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
    13081 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccggac
    13141 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
    13201 gagaataacg cgaacaaaaa taagcagtga ccctgatatt tatcagggtc actgcttatt
    13261 ttcctcccta aaacttctga aagatccggc agttttagat cactgggcaa gactcaaaga
    13321 ttttctatgt gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgatagatta
    13381 actagaggaa gcgtcttgtc ctcatccgac ctagattatt cgatattcct cgacaataaa
    13441 cgattaatca gtgagggcaa tcagaggaac ggataatgat gaataagctg taaaaaatag
    13501 ggctttgaca aaagcgtgtc acagccctta tttcgattaa acggtgtgta cctgtcaact
    13561 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
    13621 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
    13681 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
    13741 tcttattact tgatgcaggg cagatttttc acaacctctt tattcagata aaaccaagct
    13801 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
    13861 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
    13921 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
    13981 tacacctcca gctacaatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
    14041 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
    14101 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
    14161 aaaactgaaa tcataattat cttcatggat catcgctcgt ggaaaatggt aattatcttt
    14221 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
    14281 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
    14341 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
    14401 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
    14461 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
    14521 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
    14581 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
    14641 gcggctagct acttcaggaa ctacgccacc aaaacgttta tgactgttta tttgagaagc
    14701 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
    14761 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
    14821 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
    14881 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
    14941 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
    15001 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
    15061 attactttcg agtatccaat aaagccacag atctggttat cttcaactaa aagcagataa
    15121 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
    15181 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
    15241 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
    15301 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa acctaacttc
    15361 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
    15421 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
    15481 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
    15541 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
    15601 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
    15661 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
    15721 cgatcgattt gttcgattgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
    15781 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
    15841 tttttcttct gcttctaccc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
    15901 tggttcagct aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
    15961 aactgtatta atcatcgcac taggcaatat ttcctttatt tcatagaaga atttgtgaca
    16021 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
    16081 agcagaaata tgtacatctt caagtacaat ttctaatcta ttttctttcc agcggtatgc
    16141 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataattt tatccacacc
    16201 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
    16261 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
    16321 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
    16381 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
    16441 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
    16501 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
    16561 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaatgat acatgattat
    16621 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcacttcat
    16681 ttccccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
    16741 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
    16801 ctagctctcc catccattcg gtataaactt ttacagtaga aagagagggc gttacatatt
    16861 tcgctacact gacatcattg aaacccatca cagaaatatc ttctggtacc actaaacctt
    16921 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
    16981 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
    17041 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
    17101 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
    17161 caagcatagc gatatgttgc gctttatggg agacaaaatg ttcaatgact ttttgaacac
    17221 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
    17281 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
    17341 gtgcgatcgt tccatctgtc ggaagatcac taatggaatc ccacgtttct ttcaataaag
    17401 taacgtttgc ttcttctgcc tttttttcga ttcctagacg aatagataaa taataaagat
    17461 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
    17521 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
    17581 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
    17641 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
    17701 ttacgtttat aaaattgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
    17761 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
    17821 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
    17881 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
    17941 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
    18001 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
    18061 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
    18121 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
    18181 ttcatccgaa tatgctttcc acttttccaa tactgagtta gctgcttcaa tcaacaattt
    18241 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
    18301 ttgcggataa tctgctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
    18361 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa tcggcaagtc
    18421 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
    18481 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
    18541 gtaataagca taaggagaat attggaatcc ccagctttca ccgtctagat tcattcgaat
    18601 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
    18661 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
    18721 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
    18781 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
    18841 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa
    18901 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
    18961 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
    19021 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
    19081 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
    19141 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
    19201 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatt
    19261 gctttgatat ctgtgtattc aggtttccaa cccagaacct ctctcgcttt ttcacttgaa
    19321 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
    19381 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
    19441 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
    19501 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
    19561 tcgtctccat aaacagccaa tgcttttctt tgaccaagag ctacttgtaa aatgattggg
    19621 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
    19681 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
    19741 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
    19801 tctgtgattg gagactcttt tggttctccg taagttgctg cagtagaaga gaacacgatt
    19861 ttattcacat tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
    19921 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
    19981 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
    20041 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
    20101 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
    20161 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
    20221 gtgtttgttg attatatata atttaaaaaa acgaagttt
//
LOCUS       DAXWLH010000045        19607 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXWLH010000045 DAXWLH010000000
VERSION     DAXWLH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.045"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..392
                     /locus_tag="ACGE36_001168"
     CDS             <1..392
                     /locus_tag="ACGE36_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383578.1"
                     /translation="QGAEILGLIQGSGLVVSRNFPALTVFYLVISVGLLLPKSLINHY
                     NWQLLYFSCLLVFTSMFSLLPRLIIGPNGLSESGLKDSAYSSMYFLTIKPWFYLVIAS
                     FILATFFYFWTEIRIKSERSVSIKNNY"
     gene            589..1479
                     /locus_tag="ACGE36_001169"
     CDS             589..1479
                     /locus_tag="ACGE36_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFF8383579.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1805..4279
                     /locus_tag="ACGE36_001170"
     CDS             1805..4279
                     /locus_tag="ACGE36_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8383580.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4382..6118)
                     /locus_tag="ACGE36_001171"
     CDS             complement(4382..6118)
                     /locus_tag="ACGE36_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8383581.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6115..6819)
                     /locus_tag="ACGE36_001172"
     CDS             complement(6115..6819)
                     /locus_tag="ACGE36_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8383582.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6950..7453
                     /locus_tag="ACGE36_001173"
     CDS             6950..7453
                     /locus_tag="ACGE36_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFF8383583.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7413..7751
                     /locus_tag="ACGE36_001174"
     CDS             7413..7751
                     /locus_tag="ACGE36_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFF8383584.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7772..9190
                     /gene="ffh"
                     /locus_tag="ACGE36_001175"
     CDS             7772..9190
                     /gene="ffh"
                     /locus_tag="ACGE36_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFF8383585.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9302..9880
                     /locus_tag="ACGE36_001176"
     CDS             9302..9880
                     /locus_tag="ACGE36_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFF8383586.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9884..10405
                     /locus_tag="ACGE36_001177"
     CDS             9884..10405
                     /locus_tag="ACGE36_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFF8383587.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10484..11038)
                     /locus_tag="ACGE36_001178"
     CDS             complement(10484..11038)
                     /locus_tag="ACGE36_001178"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8383588.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11291..11566
                     /gene="rpsP"
                     /locus_tag="ACGE36_001179"
     CDS             11291..11566
                     /gene="rpsP"
                     /locus_tag="ACGE36_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFF8383589.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11578..11829
                     /locus_tag="ACGE36_001180"
     CDS             11578..11829
                     /locus_tag="ACGE36_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFF8383590.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11953..12744
                     /locus_tag="ACGE36_001181"
     CDS             11953..12744
                     /locus_tag="ACGE36_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383591.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12914..13435
                     /gene="rimM"
                     /locus_tag="ACGE36_001182"
     CDS             12914..13435
                     /gene="rimM"
                     /locus_tag="ACGE36_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFF8383592.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13425..14186
                     /gene="trmD"
                     /locus_tag="ACGE36_001183"
     CDS             13425..14186
                     /gene="trmD"
                     /locus_tag="ACGE36_001183"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFF8383593.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14322..14669
                     /gene="rplS"
                     /locus_tag="ACGE36_001184"
     CDS             14322..14669
                     /gene="rplS"
                     /locus_tag="ACGE36_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFF8383594.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14979..15221
                     /locus_tag="ACGE36_001185"
     CDS             14979..15221
                     /locus_tag="ACGE36_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFF8383595.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15309..15455)
                     /locus_tag="ACGE36_001186"
                     /pseudo
     CDS             complement(15309..15455)
                     /locus_tag="ACGE36_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15512..15826
                     /locus_tag="ACGE36_001187"
                     /pseudo
     CDS             15512..15826
                     /locus_tag="ACGE36_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            15855..17114
                     /locus_tag="ACGE36_001188"
     CDS             15855..17114
                     /locus_tag="ACGE36_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8383596.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17167..18063
                     /locus_tag="ACGE36_001189"
     CDS             17167..18063
                     /locus_tag="ACGE36_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383597.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPESAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18074..18943
                     /locus_tag="ACGE36_001190"
     CDS             18074..18943
                     /locus_tag="ACGE36_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383598.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            18953..19607
                     /locus_tag="ACGE36_001191"
                     /pseudo
     CDS             18953..19607
                     /locus_tag="ACGE36_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6431 a         3435 c         4065 g         5676 t
ORIGIN      
        1 tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg attagtggtt tctaggaatt
       61 ttccagcttt gactgttttc tatttagtga taagtgtagg tctcttatta ccaaaatctc
      121 tgatcaatca ctacaattgg cagctgcttt acttttcttg cttattagta tttacatcta
      181 tgttttcttt gctgcctcgc ttgattatcg gccctaatgg tttgagcgag agtggattga
      241 aggattcagc ttattcctcc atgtatttct tgacgatcaa accatggttc tatttagtaa
      301 ttgcctcatt tattcttgca acattttttt acttctggac agaaatcagg ataaaaagtg
      361 aacgatctgt atctataaaa aataactatt gatggtggat catcagtaaa aaagcatatg
      421 agctgcgtaa aatgcatgtt tctcatatgc tttttttatt attatgtaaa gaatgcgaaa
      481 aacgtttaca tgatttttac ataacttaac tatattttta cagcaaccat acgagggatt
      541 tacagtcgac tgataaactt tagttgtaaa taagaaagga gcaataaaat gaaaaaagta
      601 ttgtttactc taggtatgat gggaattctt ttagccggat gcggctcagg atcaggaaat
      661 acagatggct ctgccacaca aaataattca agtgactcaa atgaacaagt aaaaatcgtt
      721 gcggtaggtt cgacagcatt acagccgtta gtcgatgcag cacaagaaag ctttgtacaa
      781 gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa
      841 gtcgaagctg gagcagttac tatcggaaac tctgatgtat ttgcagaaga aagagacgga
      901 gttgatgctt caaaattagt ggatcataaa gtagcagtgg tcgggatggc accaatcgtc
      961 aataaagata cagatgtcaa agatatcacc aaacaggaat tgattgatat ctttacagga
     1021 aaaatcacta actggaagga agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt
     1081 gcaaacggca gcggtacccg cgcgacattc gaaaaatggg gactggatgg tgctacccct
     1141 gtgcagtccc aagaacaaga ttcatcagga acagttcggc agctggtaag tcaaacacca
     1201 ggagcaatca gctatttagc tttctcatat ctcgatgatt ccacacaagc actaagtatc
     1261 gacggtgtag aaccaaaaga agagaatgta gcagataaca gctggggaat ctggtcatat
     1321 gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc aaaaattctt agactacatg
     1381 atgacagaag aaatccaaga aggacctgtc aaagaattgg gctatttacc aatcacaatg
     1441 atggaagtag aacgtgatca cgaaggaaat atcaaataac tagcagataa acatgccacg
     1501 gtagtcgtta ctattaagga aaaatcaact atgtttcccc acatatacat ttttccttta
     1561 attgtgaaaa atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg
     1621 attgaacacc gtttattcga aaaaaagagc tacgacatag aactgtcgta gctttttttt
     1681 tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac tttccatatc
     1741 tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca atcgaggtag
     1801 ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac aaagatcatc
     1861 agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag atcaagtcaa
     1921 ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca aaacaaatca
     1981 gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta tcttagccat
     2041 tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta tcatagccaa
     2101 tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg aactaaatgt
     2161 tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa tagatataga
     2221 agaccttgta atagatgaca ttgttatttt agagactgga catcaaattc cagcagatgc
     2281 agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg aagcagatga
     2341 aataaccaaa gaaataggcg atccattaat gtccggcagt ttcattgttt ccggaaaagc
     2401 ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga cagtcaaagc
     2461 caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc aattgatcaa
     2521 atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa gttacttttt
     2581 taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat
     2641 acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga ctaaactagc
     2701 aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa gggtaaatgt
     2761 cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc aaaaagtgat
     2821 cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat gcattgctga
     2881 ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag catattttac
     2941 aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc aaaaattcag
     3001 cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga
     3061 tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt
     3121 ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag cagtcacacc
     3181 attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca catttgatta
     3241 cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc tcaccgtatc
     3301 caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg catctaaatt
     3361 atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac gcgtcaagcc
     3421 agaacaaaag aaaatcttcg ttcaattgct aaagcgtcgt aacaatacag tagctatgac
     3481 aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc
     3541 ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc
     3601 acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc
     3661 cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt
     3721 tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg
     3781 acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat
     3841 catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc
     3901 gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac
     3961 catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa
     4021 attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt
     4081 gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct
     4141 atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga
     4201 acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat
     4261 atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc
     4321 gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca gttgctcttt
     4381 tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga ctgtctacag
     4441 taatcgtacc atgaaggagt tgcacgtagt cttttacaat cgctaaccct aatccggtcc
     4501 cgcctgaatg ccggcttcgc gccttgtcta cgcggtaaaa acgctcaaag atccgttcct
     4561 gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga tcatctgttg
     4621 cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca ttttcaatca
     4681 gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg ttctttccaa
     4741 tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga acgatttgct
     4801 ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca tcttttgaca
     4861 gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc atgatctcaa
     4921 gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac
     4981 caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg aaatcctttt
     5041 gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga atctgtccgg
     5101 taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa
     5161 gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc agctgaatga
     5221 gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt accacgttta
     5281 aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca tcaatcagga
     5341 atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg gtagtatacg
     5401 cccgatacat cttactcatt tgttcgctca acgcattgat cgtctgatac aattcttccc
     5461 attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc atttttttta
     5521 gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa tagatcattc
     5581 ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga aaactctcag
     5641 ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct aactggccac
     5701 tttttttgac tggtaatgcg acatataaaa gatccccatt caaagtagta cttttgcgca
     5761 aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg gagtttcgct
     5821 gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca tccattaaag
     5881 taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt tgcgctgctt
     5941 ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc aggtagcttt
     6001 cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag ccaccaaaaa
     6061 acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc catttcatcg
     6121 tttgggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact ttgggtgttt
     6181 tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga ttcggctttg
     6241 tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc
     6301 ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt caatcagatg
     6361 gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa tcgatagata
     6421 ttcaggctca gcttcatctt ttgattgctt acgaggttca attcgtctga atattgcttt
     6481 catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt cagcacctat
     6541 ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca aaatcggtgt
     6601 ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg ttggaagcat
     6661 cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc cttcgcctcc
     6721 atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca agagcgttac
     6781 aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct ttaaataggt
     6841 ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac aaaagaaata
     6901 aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat
     6961 cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga
     7021 attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg
     7081 attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg atgtgtggaa
     7141 agatcaagca aaagtatgga cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg
     7201 cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt gatctagtca tcatgaagga
     7261 aaaaagccct tcatgcggaa cccattcgat ttatgacggg agtttttccg gaataaaaaa
     7321 aaatggtgcc ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga
     7381 tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga
     7441 acgcgctctt tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat
     7501 tatattatgc agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac
     7561 aagcagtata tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac
     7621 ttcatctttt ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata
     7681 ttacagataa gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa
     7741 tcgaagaata aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct
     7801 ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga
     7861 aatgatgcgg gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa
     7921 agattttact aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc
     7981 acctgcacaa caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga
     8041 aacagcagag ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca
     8101 aggggcaggt aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa
     8161 tgctcgtccg ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa
     8221 agtattaggt caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt
     8281 agagatcgtc cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat
     8341 cgatacggca ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa
     8401 agaattgacg caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc
     8461 cgtcaacgta gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa
     8521 acttgatggg gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc
     8581 aatcaaattc atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg
     8641 tatggctagc cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca
     8701 agactatgac gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga
     8761 tttcaatgac tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct
     8821 aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc
     8881 aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa
     8941 tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt
     9001 tgttgaagtg aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat
     9061 gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg
     9121 aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa
     9181 acggaaataa gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct
     9241 tttctatcag caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca
     9301 gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg
     9361 gggtgttcct gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca
     9421 agccggattg agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga
     9481 tcgattcgat attaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga
     9541 aaacaaagga attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc
     9601 agtcttgaaa attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa
     9661 tggaaaaatc gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct
     9721 ttcagatgaa ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac
     9781 aatctatgct tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg
     9841 caaagaagag gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt
     9901 ttatggtgct ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct
     9961 ttttgacact ccaacaggta agcaaaccac ttatgaagct ttttatgaaa caatcgatac
    10021 gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat
    10081 ttatccagag aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc
    10141 tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa
    10201 agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat
    10261 tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca
    10321 aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat
    10381 cgaattgcat tatgtacgaa agtagaagta aataaaataa aaaaggcaac tatatgcaga
    10441 aatgtcctgt acatagttgc cttttttatt accgttatgc tttttactct gttaatttgt
    10501 tagaaaaatc tagatacgca tctaaaatcg tttggaagaa acttaatgtt ccttcaacga
    10561 tattaccatt ttcatcaatt agattcgcaa cgtttccgat atatgcttcc ggttgctgca
    10621 aggttggcac attcaaaaag acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc
    10681 cgatcccacc tggtgatgca gtaacgacta aacctggttt atgatcccaa acactgtgtc
    10741 cataaggtct tgagcctaca tctaaagcat ttttcaatac tgctggcaca gaacggttgt
    10801 attcaggagt aacgaaaacg acaccgtcta gtcccctcac ttcctcacga aaacgagtcc
    10861 attcagcagg agcctgttct ggagtttcga gatcctcatt gtaaaatggc agatcatcga
    10921 tttttacaaa tacactttcg tacccttcag gaagcaattt cgcaaaagct tctgctactt
    10981 ttttgttgta agaatctttt cttaaactac caataaaaaa tccaattttt tttgtcatac
    11041 gatttttcct ccttcaaact atctataaca atcttacaaa aaataagtaa ctggagcaaa
    11101 taaaaacact tactgacaaa agctacatag aagatagatt acttattttt aacaaaaaag
    11161 aagtctaaaa tcttctattt cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa
    11221 agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac aatggaggtg
    11281 gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt
    11341 ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca tcgaaactgt
    11401 aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag acttagttct
    11461 tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc tttcaaaaga
    11521 aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg accggatatg
    11581 aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta tcctgatcaa
    11641 gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac agtggctcct
    11701 gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc
    11761 gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag
    11821 gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg tttttttatt
    11881 aattgaagaa atgattattt ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg
    11941 agtgaacagc caatgagaaa gaaaatgtta tttattccat ttgctctatt gttaagcgct
    12001 tgtagttcgg ttactcaaga atcagacaat acacatctat caagcagtat atctgaaact
    12061 gttaccagtc cagaaaaaag tagtaccaaa acggctacca ccactgaaca aacaatggct
    12121 acctcaaata atgagaaaaa gactgcttta gaccagttga aagaacaaca gccaaatgtt
    12181 cccatgcctc tagatgtacc agtctcttca ggctatctca atatcgcagc gacacataca
    12241 aagcaaggtt attctatttt atattacaga acagatcgtc ccctcgggtt gaatgctgac
    12301 gaattgaatc aagaaacgcc tattgcaacc tatctatatc aatatgggtt tgcttctagt
    12361 caagaaacaa tccaagtttt gcagcccttt gagatcgaca caaatggtca gcaagtggac
    12421 ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg
    12481 caagaaggaa actggcgtat tcgaatccgt gggaataata tagaaggcca agatccctta
    12541 ctattagcta aagaaatcgt agcttatttg gaagaaaaca gtctaccagc acctgaacaa
    12601 tttggtaaaa ttactgttga tatgggtgat acgacgaatc gagccgtaga agtaagctgg
    12661 caagaaccga aaaatgccta tacgatcact catcaagatc ctatgtctgc gttgaagatg
    12721 gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc tgagcccatc atttaactcc
    12781 aagtcacagg aagtaatcaa aaatagcacg ccgcattttt gcataatttt ggcttaatac
    12841 cttaaaagaa aactagaatt gcctcttcac acatgctaaa ataaaacaga caaaaaagaa
    12901 atggggatat aagttgactg aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat
    12961 caaaggcgaa gtccgtgtta tctcaacaac ggatttccca gaagaacgtt acaaaaaagg
    13021 aacggtgtta acactattcc aagacggaaa agcacctgtg gaattaacag tcaaaagtca
    13081 tcgcaaacat aaaaattttg acttattgag ttttgaagga catccttcta tcaatgatgt
    13141 ggaaaaatat cgggatggca tattaaaagt gtcaaaagac aactctgtag atctagctga
    13201 aaatgaattc tattaccatg agatcatcgg tctgcatgta ttagaagatg gaactgagtt
    13261 aggtaaagta aaagaaatct tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc
    13321 gaaaaaagct gatatattgc ttccatatat cgcttctgtc gtcaaagaag tcgatttaga
    13381 agctggagtg gtccgtgtag aaattccaga aggactgatt gacgatgaag attgatgtat
    13441 tgactttatt tccaagaatg tttgaaggtc ctatggggga atccatcatc ggtaaggctg
    13501 tagataaagg attattagat atcaatatct ctaacttccg cgattattca gataacaaac
    13561 atcagacagt agatgattat ccatacggcg gtggagcagg tatgctgctc aaagtccagc
    13621 ccatctatga taacatccaa gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa
    13681 ttcttcttga cccagctggt aaacgattcg atcagaaaat ggcagaagaa ttttcacaag
    13741 aagaacactt agtattcatt tgtggtcatt atgagggata tgatgaacgt atccgcacat
    13801 tagtgacaga tgaagtttcc ttaggagatt atgtccttac tgggggagaa ttaggtgcga
    13861 tggtcatgat cgatgcaaca gttcggcttt tgccagatgt gttagggaat cagacttcag
    13921 ctcaaacgga ttcttattca accggtttat tggaacatcc ccaatatact cgcccggcag
    13981 aatataaagg gatgaaagta ccagaagtat tgacaaatgg aaatcataaa ttgattgaag
    14041 aatggcaatt aaaagaatca ctaagaagga cctatcaacg tcggcctgat cttttggaaa
    14101 cacttgaatg gacacctcaa atgacgaaat ttttagaaga aatcaagaaa gaagaacaag
    14161 aaaaaacagc agatgaagcc aagtgacttt ttctaaaata ttctttacag taggttgata
    14221 atatgttagt attttatggt gagtgcaaaa gcacttaact attacgatat tccgctatga
    14281 gaaaagctca taagaatatt tggaataagg agaagaaaac catgaatcca ttaatcgaag
    14341 aattaacaaa agaacaatta cgttctgaca tcccagcgtt ccgccctggt gacactgtgc
    14401 gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat ccagttattt gaaggtgttg
    14461 ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac agtacgtaaa gtttctaacg
    14521 gagtgggcgt tgaacgtaca ttcccattgc atacaccacg tgttgcaaaa atcgaagtag
    14581 ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct acgtgcatta cacggaaaag
    14641 ctgctcgtat caaagaaatc cgtcgttaat cattgttatc agactgagaa agaacccttg
    14701 gtattcaagg gttctttttt cttagcatgt aatttgaaca ccctgatcca gtttttttct
    14761 ttaattattg gtaggggaga ataatggatt agaataaatc tacctataag tagtagtaaa
    14821 aaaaagagca ctatgataat atctttctta agagtaaccg ctttaataaa aaatccagaa
    14881 tattctaaca taaatggagt attcttagaa tcaaaaggag aaagaagatg gtttatactt
    14941 atacggagca agaaaaaatg aaagtttgag acacattcat gaatgatgga aaagtaaaac
    15001 aagtaccaag ttcaacaaaa aagaaaaaca tcctattgaa agaagtcttg aaacgctttg
    15061 accatggagt gacttataca gaaacagagg ttaattcgat actgctgaac gtattttcta
    15121 gtggcgatta tgtcgaacaa agacgttatc taatcacatt tggctttttc aaaagaagtt
    15181 cagacggtcg ggcatatcag atgatgggaa ttgaaaatta gaagttttaa tttatactca
    15241 ttgaaaaaag ttcaagtatg cgatattggt tctacaccgc atacttgaac tttttttgtc
    15301 atctgtatct ataatacaaa gccaagaacg ttattcaaaa tatggatagc tatgataaaa
    15361 gctaatgagc gattcgcagt taaataagca atcgtcagta aaatcgttcc aggagcgtac
    15421 atgaaaaaat caattggatg cgttacatga ataaagataa ttctctttta tttgtacgaa
    15481 taaataccaa aacgtacctc ctaaaattag attcaaagaa gatagcaaag aggaaaaaga
    15541 tagttctgca gcagctatag cggcatgtgg attgctagaa ctttccaaac aactgcctgt
    15601 ttcagatcct cgtcatttcg attacgaaga gatagcaatc aaaatcttgg aagaattaca
    15661 gaaaaactat acaacgaaac gagttaacgg atcaaacggt ctgcttctcc atgccgttta
    15721 tgataaaaac agcttaaaag gtgtagatga atgtgtgatc tggggcgatt acttttatgt
    15781 agaaggaatc actcgtctag ctaaaacatg gtactgttac tggtgatgta taagagaaga
    15841 ggaggattca tataatgaag cgatggaaaa aaatgatgat tggatcaata gttttaggaa
    15901 tgtcactcgt atttggtgct tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg
    15961 aaattaccgt ttcgacatgg aattatgaaa caacacctga attcaaagcg ctctttgaag
    16021 cattcgaaaa gaaaacggga atcaaagtga aagcagtaga tattgcttca gatgattatg
    16081 atacgaaact caccacgatg ctttcttctg gagacacgac agatgtattg acgatgaaaa
    16141 atcttttgtc gtattcaaat tatgctttac gagatcagtt agtcgatcaa acagaacgaa
    16201 ttagagaatt agacactgat gctgctgaag aaacatatga aatgtatgat atcgatggaa
    16261 aaacatatgc attgccatat cgaacggact tttgggtact atattacaat aagaaaatgt
    16321 ttgatgatgc aggaatcgaa tatccaaaaa atctaacttg ggacgaatat gaagatttag
    16381 caaaaaaact tagtaaaaat gacggacaag tttacggagc atatcaacat atctggcgtt
    16441 cgacaatcca agctattgca gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc
    16501 aatatatgac tgattattat gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg
    16561 attttggcac ggctaaatca acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag
    16621 cagcaatgat gtacatggga acttggtata tggctggcat actcgcaaac aaagaagcaa
    16681 gtaagacaga agtagaatgg ggaattacag ctattcctca gaagaaaaaa ggagaatcgg
    16741 tcacgacgtt tggctcacca acagcatttg ctgtcaataa aaactcaaag aaacaaaaag
    16801 cggcgcaaga atttattgaa tttgcagcca gtgaagaagg agcaaaagta ttagcaggag
    16861 taggagtagt tccttcatat cgtacggatg agattgatca gctttatttt aatctcgctg
    16921 gaatgccaac ggatgaaatt tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat
    16981 ttccgattga tacgtatggg cctgcaatcg ataagatttt acaagaagaa tatgacttag
    17041 ttttggttgg agatgaaacg ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg
    17101 cggaaaaaga ataatcatga aaggagagaa gccaagagat aaaacactct tggtttcgtc
    17161 atcgaaatgg agaatagtat aaataagaaa gctacaaatc atttgcgtag aaaacgaacg
    17221 tggatcgctt attcgtttat cgccccgaat ttcattggtt ttttcgtttt tacattaata
    17281 ccaatcctgt tttcgcttgt actagcattt atgaaatggg attctttttc aacgccagaa
    17341 tctgctggac tagataactt tcgtaaaatg gccaacgatg agacattttg gatttctttg
    17401 aaaaatactt ttttatatac cgctgctgta gtgcctggca cacttgtttg ttcgttaggg
    17461 ctagctttac tactaaatca gaaaattaga gggatgaagc tttttcgtac gatatttttc
    17521 tttccgtatg ttacttcctt agtagcaatt gctgttgttt ggaatatgtt gtttcatcca
    17581 acagtgggac ctattaatat ctttttaaga cgatttattg aacatcctcc aggttggact
    17641 tcaagttcaa attgggcgct ttgggcaatt gtaattgtca gtatttggcg agggatgggt
    17701 tattacatga ttctatacct tgcaggacta caaagtatcc aaaaagaact atatgaagcg
    17761 tcttcgctag atggagcaaa ccgttggaaa caatttttga atgtgacatt accttcgttg
    17821 agaccaacaa ccttctttgt aacgatcatg cttgtcatca attgtttcaa ggtgtttgac
    17881 cttgtgcaag tgatgacgga tggcggccct ggtcgagcga caaatgtgct ggtttaccaa
    17941 atctacaatg aagcattcaa aaaatttgat tttggttatg cttcagctat ttcacttgtt
    18001 ttgttcgtaa ttgttttggg cgttacggtt gttcaatttt cttggaataa gcgtcgtact
    18061 taggaggtgt gatatggaag agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc
    18121 aaaaaacatg ccctttattc aatggttggg tatgattctg ctgattgtat tagctttatt
    18181 gacgatcatt ccttttttat ggatggtttc tgcttcattc aaagcaaata atgaagtgtt
    18241 aacgattccg attcaatgga tccctaaaac ttggcattta gagaattatc tggttatttg
    18301 gtctcgtatt cctttagtga cattttttaa gaatacagct tttttaagcg ttgtgatcac
    18361 gttgattcag ttgtttactt ctagttttgc tgtttacggg ttttctgaac taaattttaa
    18421 agggcgcgat attttatttt tagcatatat cggcacgatt gctgtccctt ggcagtctta
    18481 tatgatcccg cagttcatta tgatgagaaa agtgggttta acagatacgt tgtggtcgtt
    18541 ggtgctatta caagcgttca atgcttttgg tgtgttcttg ttgaagcaat attacagtag
    18601 tatcccagat tctttatgtg agagtgcgcg gatcgatggc ttatcagagt gggaaatcta
    18661 tcgcaagatc attttacctc ttacaaaacc ggcgattgcc agtttgacga tcatcacatt
    18721 cgttaataca tggaacgatt atatggggcc attcatttat ttgtcttcta cggaaaataa
    18781 aacaatccag ttagggttga aaatgtttat tgggctattt gatgcagagt acgcattaat
    18841 catggctgcc tcagttgttt ctgttcttcc tgttgctttt atatttttag cgatgcaaaa
    18901 atattttgta gaagggatcg cttcatcagg aatgaaggaa taggaggtga ttatgaaaga
    18961 gcagcgtttt gacaaaaatg tttatatgga attcttttat tggttgtatc aatcattggc
    19021 aagtagcctt tgtttttggt gtgtcaatat cttgtatata gcagctgcgt tccttttgtc
    19081 gatagatgga aggaatgtct tttcctttat tttttcttta ctttttattg gtccaggttg
    19141 catcgtgatg ataggtatga cagaagaatt agcaaaagaa aaaagttttt caccagtacg
    19201 tttgttcata aagacgtttc gccaattttg gctaaaggga tttttttatt ggctatttgc
    19261 ttggatagtt agtgtgataa tgatttttga ttgtttcttt tttatacgtt tttcttatgg
    19321 aaaatggttg attccgttgt ttgttttact tgcttgtttt acttgcttgt ttatcagttt
    19381 cctttagcat caactgctgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag
    19441 ttctacggat agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt
    19501 tactattgac aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag
    19561 gtccttctat cgtatttggt ttgatttact tcaacaatcg taaattg
//
LOCUS       DAXWLH010000046        19594 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXWLH010000046 DAXWLH010000000
VERSION     DAXWLH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.046"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            133..3660
                     /locus_tag="ACGE36_001192"
     CDS             133..3660
                     /locus_tag="ACGE36_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFF8383599.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACGE36_001193"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACGE36_001193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFF8383600.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACGE36_001194"
     CDS             7448..7789
                     /locus_tag="ACGE36_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8383601.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE36_001195"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE36_001195"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFF8383602.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE36_001196"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE36_001196"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFF8383603.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE36_001197"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE36_001197"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFF8383604.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACGE36_001198"
     CDS             13000..13737
                     /locus_tag="ACGE36_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8383605.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACGE36_001199"
     CDS             13751..14566
                     /locus_tag="ACGE36_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8383606.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACGE36_001200"
     CDS             14579..15226
                     /locus_tag="ACGE36_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8383607.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACGE36_001201"
     CDS             15242..15898
                     /locus_tag="ACGE36_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8383608.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACGE36_001202"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACGE36_001202"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFF8383609.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACGE36_001203"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACGE36_001203"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFF8383610.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACGE36_001204"
     CDS             18240..18758
                     /locus_tag="ACGE36_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8383611.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACGE36_001205"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACGE36_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFF8383612.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6578 a         3364 c         4205 g         5447 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggcaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gtgaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc ttta
//
LOCUS       DAXWLH010000047        18454 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXWLH010000047 DAXWLH010000000
VERSION     DAXWLH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.047"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..610)
                     /locus_tag="ACGE36_001206"
     CDS             complement(<1..610)
                     /locus_tag="ACGE36_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8383613.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTE"
     gene            complement(632..1606)
                     /locus_tag="ACGE36_001207"
     CDS             complement(632..1606)
                     /locus_tag="ACGE36_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383614.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1906..2778
                     /locus_tag="ACGE36_001208"
     CDS             1906..2778
                     /locus_tag="ACGE36_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8383615.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(3236..5434)
                     /locus_tag="ACGE36_001209"
     CDS             complement(3236..5434)
                     /locus_tag="ACGE36_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383616.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            5666..6658
                     /locus_tag="ACGE36_001210"
     CDS             5666..6658
                     /locus_tag="ACGE36_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8383617.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            6718..8139
                     /locus_tag="ACGE36_001211"
     CDS             6718..8139
                     /locus_tag="ACGE36_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFF8383618.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            8132..9631
                     /locus_tag="ACGE36_001212"
     CDS             8132..9631
                     /locus_tag="ACGE36_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFF8383619.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            9670..11064
                     /gene="uxaC"
                     /locus_tag="ACGE36_001213"
     CDS             9670..11064
                     /gene="uxaC"
                     /locus_tag="ACGE36_001213"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFF8383620.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            11223..11906
                     /locus_tag="ACGE36_001214"
     CDS             11223..11906
                     /locus_tag="ACGE36_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFF8383621.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            11908..12744
                     /locus_tag="ACGE36_001215"
     CDS             11908..12744
                     /locus_tag="ACGE36_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8383622.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            12755..14509
                     /locus_tag="ACGE36_001216"
     CDS             12755..14509
                     /locus_tag="ACGE36_001216"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383623.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(14785..15126)
                     /locus_tag="ACGE36_001217"
     CDS             complement(14785..15126)
                     /locus_tag="ACGE36_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8383624.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(15194..17365)
                     /locus_tag="ACGE36_001218"
     CDS             complement(15194..17365)
                     /locus_tag="ACGE36_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8383625.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            17576..18433
                     /locus_tag="ACGE36_001219"
     CDS             17576..18433
                     /locus_tag="ACGE36_001219"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8383626.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
BASE COUNT         5612 a         3667 c         3423 g         5752 t
ORIGIN      
        1 tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct gtccacgtga
       61 ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt gtttggatga
      121 tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga agatgtgaga
      181 atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca taatgagcca
      241 tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata tacaaagcga
      301 tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc ccgatggcgc
      361 catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat gtgaagtacg
      421 ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact gcaaccatcg
      481 catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa ccgccccaca
      541 gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt ttatctaatg
      601 actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct cctagctttt
      661 tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta aacaatccca
      721 ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata taggtgtcga
      781 aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc tcgaacccta
      841 agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga cgaattgccg
      901 gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct gcagcttcat
      961 acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac cagcccaact
     1021 ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat gttagaaaac
     1081 tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg tcaacattca
     1141 aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg atgtaaatga
     1201 tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt aaagacaaaa
     1261 tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac gtatttctta
     1321 ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa ccgacccaag
     1381 gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg agtcccccca
     1441 taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc acatataaaa
     1501 atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt tcttcttttt
     1561 tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc ccctccttag
     1621 ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat tacgtatata
     1681 atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct atacaaaacg
     1741 ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt ttttctatct
     1801 gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa attgaacata
     1861 tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa aaagaaagtc
     1921 tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt gctagctttg
     1981 atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac acaagaagtc
     2041 gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta tcgctacatt
     2101 ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac aggtgtcgga
     2161 acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag aaagtatttg
     2221 tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag cggggggatg
     2281 attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg ggccatgatc
     2341 ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt ccaagccttg
     2401 ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg tgtatttttc
     2461 aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt ttatgctgtt
     2521 acttactgga acacttatat gactgctatt ttgtatatca acgattcatc taaatggccg
     2581 atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc agaaagtagt
     2641 gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt tattgcaaca
     2701 gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg agcactcgta
     2761 ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc agaagtggtc
     2821 agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg tgaaatttcg
     2881 gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt gaaagagacg
     2941 ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac ccacaggagc
     3001 aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc cttattgtcg
     3061 atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag acttttgtca
     3121 cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa atactagggt
     3181 tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc ttgattcaca
     3241 aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag aaacgaataa
     3301 aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag aggttagtct
     3361 ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca ccaaattttt
     3421 ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc aacctatcag
     3481 cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc ataatattct
     3541 ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg attggcagaa
     3601 ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc agtgctttta
     3661 cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac tgtcctaatt
     3721 gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga tttttatttt
     3781 gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga tttccagaac
     3841 gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt gttttttcat
     3901 gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta tttacacgat
     3961 aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc gtttcgaacc
     4021 ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt ttttgaatga
     4081 gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc attcgataaa
     4141 tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc tttggcacaa
     4201 tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca ccgtgactat
     4261 cttctaacag atcgatttga accaaagctg tataatagca gtcattttga gaactataac
     4321 caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta aataaactaa
     4381 gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt aacgcttctt
     4441 tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc ccactgaatc
     4501 cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca aatctgtccg
     4561 aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact gcaactagcc
     4621 cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct aatctgtaaa
     4681 tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct cttatcactt
     4741 tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct ttatttttta
     4801 ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg tcttctccac
     4861 tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc aatgcatatt
     4921 tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt ttcttttgcc
     4981 aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt tcctcttcgg
     5041 tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg actaaatcat
     5101 aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg atttcagaat
     5161 aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa tttttggtcg
     5221 aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg ttagttttaa
     5281 cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat tcataggtat
     5341 tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa aacatcggta
     5401 aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc ttttaaaata
     5461 aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca ttccccgctt
     5521 tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg caatcttttc
     5581 atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa
     5641 gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta aagcagctgg
     5701 cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa aaaatgaaac
     5761 aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact atagtgcaaa
     5821 aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt tgcataaagg
     5881 aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat tagacacaaa
     5941 ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca acctgcaacg
     6001 ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca tcgactatct
     6061 caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc cagcggttat
     6121 caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg gtattggatg
     6181 cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga caaaagaacg
     6241 ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc cagcctattt
     6301 tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc tattatcact
     6361 ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg gtgcattgag
     6421 agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg ggttcgatga
     6481 ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga
     6541 aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga cgatcgaaca
     6601 atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac
     6661 tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga agtttatatg
     6721 aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt
     6781 ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct gaataaccaa
     6841 caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct tggaaatatg
     6901 atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa tggcgaaatc
     6961 gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta tgatgaatgg
     7021 gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc caatacaaca
     7081 gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc acagagcctc
     7141 ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc tggctttacc
     7201 atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat cattttacgc
     7261 tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa agaaaatgtt
     7321 ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt
     7381 ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc attcatgctt
     7441 tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca ggctggcttg
     7501 aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca tttgcttaat
     7561 ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac tgtagaagaa
     7621 gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct agaattgatt
     7681 cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat caaagaacgt
     7741 ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag tgtttcaaag
     7801 ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa tcaggtacca
     7861 ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga tcaagtaaag
     7921 ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga taatggaaca
     7981 gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc tcaaataccg
     8041 aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg tgtccggtca
     8101 gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac caacaatgaa
     8161 attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac aaacacaact
     8221 gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc ataagattgc
     8281 attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa tcggtcatgt
     8341 gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa ccaatctttc
     8401 tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc tttttgagaa
     8461 ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt
     8521 tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa
     8581 ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc atccttatgg
     8641 ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg acgcagtaaa
     8701 tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca atacagttcc
     8761 tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc tagtggctca
     8821 agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt taacagcggc
     8881 ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg
     8941 cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat tttctgacta
     9001 cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga
     9061 acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg ttgacttgat
     9121 caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa acccatctcc
     9181 tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct gcacgcaaaa
     9241 atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg
     9301 gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc ttgcttcatc
     9361 cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct catatgtccc
     9421 aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt ggatggattt
     9481 taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa
     9541 aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat
     9601 tgccattttt aaaaacggag taacactttg attttcataa aacagtataa aaagaaagaa
     9661 ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc gaaaaagctt
     9721 taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt agatccaaaa
     9781 gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg aggcgatcat
     9841 tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct
     9901 tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat cggaaatccg
     9961 ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt gttgataaat
    10021 gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt
    10081 cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac agatgatccc
    10141 gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt gaaggttcta
    10201 ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa tgcatgggtc
    10261 acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact gatagaagtc
    10321 ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga ccacgcttta
    10381 gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt tcaaaaagca
    10441 cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga aacactgata
    10501 catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat ccatgcttat
    10561 cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta tgatggaatg
    10621 aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga agaaacggat
    10681 cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc gattttagct
    10741 ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc tggatggtgg
    10801 tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga tggtagtgct
    10861 ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta cactagacat
    10921 gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca
    10981 cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac aaatgctaaa
    11041 aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc aaaaacagga
    11101 tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac caaatttgct
    11161 ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa agaagagata
    11221 acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat gaaaacaagc
    11281 gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag atccagatca
    11341 ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga aggttagccc
    11401 gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga cataacgacc
    11461 agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa aatctgcaga
    11521 tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca tctgtgacgc
    11581 gacgagatta cttagaaaac ggaacattga atccagatat acttggagac tatccaaaag
    11641 cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc tcaagatcac
    11701 aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg catcttatac
    11761 ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt agtccagaag
    11821 gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt ctgcctcttg
    11881 ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat ccgtgcccac
    11941 gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct tcacttgtcc
    12001 catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac tgacatgaca
    12061 cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga actttccatt
    12121 ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca agcattggca
    12181 actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat tgctacggaa
    12241 accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc ttatcagatc
    12301 gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt aactgttgct
    12361 gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg tttaatcgat
    12421 gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat gcataaagaa
    12481 aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca tggttatatt
    12541 acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat ggtacctgtt
    12601 ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga gcgtcctcta
    12661 atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat agaacacttg
    12721 cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac agaaaagtta
    12781 tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg gaaattattg
    12841 gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt ggttaatgcc
    12901 gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc agttgcaggc
    12961 catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat tgacgaatta
    13021 ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac taagagcgac
    13081 ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc aatctggacg
    13141 aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct ctcaagtatt
    13201 tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca cgctttataa
    13261 aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat tcgttactct
    13321 ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa tggaagagac
    13381 ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc atgggttaga
    13441 aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa aaggattcca
    13501 cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc agatatttca
    13561 gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc aacccggcga
    13621 tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg gaatgatcac
    13681 gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg aactgttgat
    13741 tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg atggaaattt
    13801 tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta tcgatcattt
    13861 ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg gctctggaaa
    13921 atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa aaatttttct
    13981 agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt tagccgttgt
    14041 tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt atggcttgaa
    14101 aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt cttcactgat
    14161 tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca agctgtctgg
    14221 tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa aaatcatttt
    14281 gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc agcaagccct
    14341 gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt caacgatcaa
    14401 agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag gtacttacga
    14461 aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac aaaaaatagg
    14521 agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt ctcctgccaa
    14581 ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt gaaattgttc
    14641 cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg ttcaagagac
    14701 agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc tctttcacac
    14761 cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg gtgtcatgat
    14821 gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca tcaagtcatt
    14881 gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg catcttcgtc
    14941 gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg cttggaagtc
    15001 tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt ctttcaacgc
    15061 tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat cgtagatgtt
    15121 tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat ggggttattc
    15181 gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc gcttcaccaa
    15241 ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct tttgcttgtt
    15301 caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa tttcctgtgt
    15361 ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct gctacactaa
    15421 atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag actttcccga
    15481 caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg taacccatcc
    15541 atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt gcttttgtgg
    15601 catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct gcaggattcg
    15661 cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt tcatcggctg
    15721 tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc accgctccag
    15781 tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca ttggcaaatg
    15841 cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca ccaagggcta
    15901 agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct ttatcatatc
    15961 ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa ctttgtgcaa
    16021 cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc gcatcgtaat
    16081 aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc agtgctggtg
    16141 tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt gtagcaaagc
    16201 tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca ccacctgtat
    16261 tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca tttgctggga
    16321 ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga tctaaagctg
    16381 tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac tcgttaactg
    16441 cttcatcgat cactgcatca aaataataag gataacgata accagaatct gaattatcgt
    16501 atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct tgttcttttg
    16561 tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca ttcgcatttt
    16621 ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc aaagtagcag
    16681 cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa gcgtcttgga
    16741 caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag atttcttctt
    16801 tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt cgatcaaacg
    16861 tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta cttcctccgc
    16921 cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc cctttgatat
    16981 caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca tcttggatat
    17041 agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt gtgccagctt
    17101 cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt aacgtttcga
    17161 catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca cagatcaaac
    17221 caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt cgccatcgat
    17281 ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca gcttttatct
    17341 tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat tcaatcgtct
    17401 tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca atatgttttt
    17461 ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt tttttcttaa
    17521 atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta cacttgtgaa
    17581 aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact tgcttgaaaa
    17641 ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa acgtttggat
    17701 caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga tcacattgtt
    17761 attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga agcacatccg
    17821 tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa tgaagactca
    17881 tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt atctcgacac
    17941 taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg gtgccgtttt
    18001 atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac aaaaaatgat
    18061 ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga ccatcaccaa
    18121 taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata aaaatggtca
    18181 gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac aagtctattg
    18241 tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata agggacatcc
    18301 gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc tccatgcatt
    18361 agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag ctttacctgg
    18421 attatgggaa tagaaataaa aaagaacaga gtct
//
LOCUS       DAXWLH010000048        17976 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXWLH010000048 DAXWLH010000000
VERSION     DAXWLH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.048"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..283
                     /locus_tag="ACGE36_001220"
     CDS             <1..283
                     /locus_tag="ACGE36_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383627.1"
                     /translation="GVGILSLFFQFKKRSIGSQREKWSQYFDKIGQKGLVTRLHISYM
                     VALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKYQLNKNHLNRTFR"
     gene            complement(400..726)
                     /locus_tag="ACGE36_001221"
     CDS             complement(400..726)
                     /locus_tag="ACGE36_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383628.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(1099..2300)
                     /locus_tag="ACGE36_001222"
                     /pseudo
     CDS             complement(1099..2300)
                     /locus_tag="ACGE36_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2417..2932
                     /locus_tag="ACGE36_001223"
     CDS             2417..2932
                     /locus_tag="ACGE36_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8383629.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(3131..3577)
                     /locus_tag="ACGE36_001224"
     CDS             complement(3131..3577)
                     /locus_tag="ACGE36_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8383630.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3624..5102)
                     /gene="msr(C)"
                     /locus_tag="ACGE36_001225"
     CDS             complement(3624..5102)
                     /gene="msr(C)"
                     /locus_tag="ACGE36_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8383631.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(5456..6085)
                     /locus_tag="ACGE36_001226"
     CDS             complement(5456..6085)
                     /locus_tag="ACGE36_001226"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8383632.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(6069..6533)
                     /locus_tag="ACGE36_001227"
     CDS             complement(6069..6533)
                     /locus_tag="ACGE36_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8383633.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(7020..8288)
                     /locus_tag="ACGE36_001228"
     CDS             complement(7020..8288)
                     /locus_tag="ACGE36_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8383634.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(8519..9256)
                     /locus_tag="ACGE36_001229"
     CDS             complement(8519..9256)
                     /locus_tag="ACGE36_001229"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8383635.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(9323..9892)
                     /locus_tag="ACGE36_001230"
     CDS             complement(9323..9892)
                     /locus_tag="ACGE36_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8383636.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            10020..10892
                     /locus_tag="ACGE36_001231"
     CDS             10020..10892
                     /locus_tag="ACGE36_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8383637.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            11234..12106
                     /locus_tag="ACGE36_001232"
     CDS             11234..12106
                     /locus_tag="ACGE36_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8383638.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            12103..14061
                     /gene="nagE"
                     /locus_tag="ACGE36_001233"
     CDS             12103..14061
                     /gene="nagE"
                     /locus_tag="ACGE36_001233"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFF8383639.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(14312..15163)
                     /locus_tag="ACGE36_001234"
     CDS             complement(14312..15163)
                     /locus_tag="ACGE36_001234"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8383640.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            15286..15753
                     /locus_tag="ACGE36_001235"
     CDS             15286..15753
                     /locus_tag="ACGE36_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFF8383641.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(16016..16498)
                     /locus_tag="ACGE36_001236"
     CDS             complement(16016..16498)
                     /locus_tag="ACGE36_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8383642.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(16598..17461)
                     /locus_tag="ACGE36_001237"
     CDS             complement(16598..17461)
                     /locus_tag="ACGE36_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8383643.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         5375 a         3730 c         3177 g         5694 t
ORIGIN      
        1 gggagtcggt attttatccc ttttctttca attcaaaaaa cgaagcatag ggagtcaacg
       61 ggaaaaatgg tcgcagtatt ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca
      121 cattagttac atggttgcat tatgcttatt agcgacaatc aattactatt cttttttcga
      181 tcattcaatc gcttatacga ttacgctact gattgctggg atttttcact tatcttataa
      241 atatcagctg aataaaaacc acttgaatcg gactttccgc taaaaaatat ccgtacgata
      301 agtagagtgc cttgaagatc ggcaaaaaac tgccggactt tttagaggct gtgacaaaga
      361 ttttgtcaca gcctcttttt ccatataaac ggcgttcgcc taccagctgc ctggtaaatc
      421 tgtttcctct tcgttaagaa gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag
      481 cagctcatcc atggacaagt tcatcgtttt ttctttttct tcttccgctg attcagccag
      541 tagctgttcc cgatacacct gatacatacc agctattttt tgctgcatcg cttttctagt
      601 ttcaccttcc gcattgatta ttggcaattc tttgatatag ccacgataaa tcaaccgatc
      661 atctgaaaat tcaatgggta attcaacgag tgtcaacccg acttcctgaa aatattcttc
      721 cagcatctat tatcccccca ttctttttct taaatgaatt ttagcttacc tactgaaaat
      781 tttccagttt cattattttg agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc
      841 tttttccgaa taaatggtgt tcttctgcca actcctcaat cataagtcac aatattcaac
      901 aaacatgaga aactgttttt tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc
      961 acaacctctt ggtaaacggc gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
     1021 gaaaaatatg agaagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt
     1081 ttcacaacct ctgatttctt attcttcttc atttgtagca aaataattac ggtgataaat
     1141 gatacaacat acactgataa gaacaatggc acctgcaaaa taaaacgcat agctttcacc
     1201 aatatgttca ccaatcacac ctgcaataaa tggtgctaaa gctccaccga aaaagcggac
     1261 aaaactatag ctggacgaag caacgtttcg ttgcagacct ggtatttcca tagcaatcgt
     1321 cgtcaacagt gtattgataa ttccttggaa aaatcctgct aacacaatgc tgatcggtac
     1381 aaggactggc atagaagttc cagctcctaa taacagcaaa caaataatga ataagacaaa
     1441 tgctgctaaa attgtccgat aagtcgtaaa cttccgctca agagccggtg caacgaaaat
     1501 cgaagcaata gccagaagca caccccagcc aaaaaataca aaaccgactt ctaattcaga
     1561 atagccaatc aataaaaatg gcgcatacgc aagtaaagta aagaatccga aattatataa
     1621 caatgcgatt agtccaactg aacgaagttt atgattgctt aacgctttga caccttccca
     1681 aaggcctact tttctctttg gtttctcagt ttcaggtaaa agtaccgcta gggctataaa
     1741 accgcaaaac atcaaaaatg ctaccccaaa aaatggataa cgccatgaac cactgcccaa
     1801 gaccccaccg actagagggc caacagccat tccgcagcca agtgctgctt catacatcat
     1861 gattgctttt tctgtgttac caactaaaat actgacgatc gttgctaaag cagtagagac
     1921 aaataaagca ttcccgacac cccagccggc acgtaaccca atcagcattc cgatattccc
     1981 agataatccg ccaagaccag cgaatacaac gatgataaac aatcctgtca tcaatgtttt
     2041 ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc agtgactaac
     2101 atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc
     2161 gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca ggtagctgct
     2221 acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg tatatctatt
     2281 tgattctcca atatttccat ccctttctct ctattagcat catacatgat ttattttaca
     2341 tgtaaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag aaaataagac
     2401 gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc ttttatccaa
     2461 accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag cccatcacag
     2521 tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg ttttggacaa
     2581 agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg ctcttgtaga
     2641 ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat
     2701 ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca ctagagaaca
     2761 acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct cgctagacaa
     2821 gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt gtcagcagga
     2881 actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt aagggataaa
     2941 cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa gagcaataca
     3001 gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg atcactattt
     3061 ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt tttcaaataa
     3121 cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa agcgggccaa
     3181 cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca ttcatataag
     3241 cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa gacatacatg
     3301 tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc ttgctttctg
     3361 gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg aagacatcca
     3421 aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat aaatgaagat
     3481 cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa cacatcattg
     3541 ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc tttaaatttg
     3601 tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt tattttctgc
     3661 ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc atgagaagta
     3721 aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat cgttgccata
     3781 tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt aaaaagcaaa
     3841 gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt cgtcaaagtt
     3901 tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga aaactctgtt
     3961 tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc cttttgctga
     4021 tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt gcggatatgt
     4081 tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa
     4141 gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa ttggacattt
     4201 tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg aaaacggatt
     4261 tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct ctctaatgtt
     4321 ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga agacaaccga
     4381 tccggtctga tttgttgctt tttcttttta ctggacactt tttccaactt ttccgcttgt
     4441 tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat cgactctgct
     4501 tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact ataattcccc
     4561 gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag ctcatctaga
     4621 aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag ctcttcaatc
     4681 agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag catcccttgt
     4741 tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc gctccatttc
     4801 cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa atttaggtcc
     4861 tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc tgtttggatt
     4921 ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga tttaccgaca
     4981 ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga atcaatagat
     5041 agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc taaattttcc
     5101 atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa aaagagcaga
     5161 aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga aataactatg
     5221 actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga aaaaatggac
     5281 atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta gttgttattc
     5341 aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt ttttattagg
     5401 ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa accttttatt
     5461 cgaacgtagt gatcttttct ggtgctaaac gaacagactc gtaaccttct tctaccgctt
     5521 ttcctattga taaaataata actggaacat aacggtcttt atccaaacca aatgcctctg
     5581 ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga tcacgcgcaa
     5641 ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg ttcatttctt
     5701 ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct ctgacttctt
     5761 ttggcatctt ccctgattcg taagcttggt tatagatttc ttcgccatat tcgtaacatt
     5821 gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc gtattgaaac
     5881 ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca aagcgccatg
     5941 gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt
     6001 cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca gtcataattg
     6061 ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt tgttatattg
     6121 caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc tacatcgctg
     6181 aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg ctcgattttc
     6241 ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg atttctctga
     6301 cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc tccttgatca
     6361 atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt cagtctcatc
     6421 atgatctcgt atctagtaag acttataccg atattttctt caaagatctg gacgattttt
     6481 tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga cataatttcc
     6541 tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa
     6601 aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag tatagagaaa
     6661 agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc taaaaccaag
     6721 attattgatt cttatcaaac tatagaagat agctcttctg cagtagaaaa ttttacaccg
     6781 ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa attggatcag
     6841 tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt ttttgagtaa
     6901 acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa acatgaaaat
     6961 ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac tacctctttt
     7021 taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag ccaatctctt
     7081 tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt aaatagtcga
     7141 taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca accgattttt
     7201 gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact gtaaggattg
     7261 tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc
     7321 cagcctacag ttggcagata atttttttct ccggcattca atgtatataa atgttctcct
     7381 gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt atcatactgt
     7441 gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg cgcagtggca
     7501 taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct tttccatgca
     7561 ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt tacgttatct
     7621 tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt aatccattgc
     7681 ccattcacat attccgaagt aggcatcgta acagattggc cattatagct tcctttaatc
     7741 ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc actttcaacg
     7801 atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa actttgtgca
     7861 gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg agcataaaaa
     7921 gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg tgtcaatgtt
     7981 tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt ctgagcttct
     8041 gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt
     8101 gaaattgtat tttctgagac agtagctgac tgcaaagtac tctctgtatc gttttcaatt
     8161 cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc
     8221 actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa taaattcttt
     8281 gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag tgtaactaaa
     8341 aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt gataaatttg
     8401 ctttttctac ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg
     8461 atggtcgttt gaataactat ttaacaagta attctctttg gatattatcc gttatggatt
     8521 atacttcctg tgtcgtcgga cggataatca tgttactgac agacatccgg tctggcgttt
     8581 ctatcgcaaa tgctacagca gaagctatat cttcggaggt caatccccat tctttttgcg
     8641 cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa
     8701 cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc
     8761 cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa
     8821 caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga
     8881 ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt
     8941 gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt
     9001 acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg
     9061 caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta
     9121 ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta
     9181 atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt
     9241 ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact
     9301 atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag
     9361 ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa
     9421 caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag
     9481 catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt ggatcggtta
     9541 gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga
     9601 tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa
     9661 tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt
     9721 cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca
     9781 gcgctgctaa aggctcttcg ttttcagctt ggatctgatg gatcacttca aagagcggat
     9841 ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc
     9901 ctccttttat ccttacttta taacggacgg cttatcatgt ctaatactta taaaacatac
     9961 ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa
    10021 tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag
    10081 cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag
    10141 aagaacttgg tgtgacactt ttcactcggg gctcccgaga gatacagttg acggattctg
    10201 gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca
    10261 atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag
    10321 caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt
    10381 ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg
    10441 attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag
    10501 cccaagatcg ttggggactt ttgatacatc gaaatcatcc tttagcaaag caaaccgtca
    10561 tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca
    10621 gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata
    10681 acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag
    10741 atgggatcat caatacttat gggacggaaa aaatattcat cccactcgat ccgccattac
    10801 aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat
    10861 ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct
    10921 ttttaacaac gaataagata agcaaaagat ccaacgatta aataacataa gtttcttaat
    10981 ttatttcttc cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat
    11041 tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga
    11101 ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg
    11161 tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa
    11221 aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga
    11281 cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga
    11341 tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa
    11401 gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat
    11461 tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac
    11521 cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact
    11581 tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga
    11641 gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat
    11701 ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac
    11761 gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga
    11821 accattttca ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac
    11881 ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata
    11941 cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct
    12001 cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc
    12061 aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa
    12121 cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta
    12181 gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct
    12241 ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg
    12301 atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca
    12361 gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt
    12421 gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg
    12481 gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc
    12541 ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta
    12601 ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt
    12661 ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga
    12721 ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt
    12781 aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc
    12841 ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca
    12901 gaaaacaaag caaaaacagc ttccttgatg ctagcagcag cttttgcttc attctttact
    12961 ggtgtgacag aaccacttga attttcattt atgttcgttg cttggccatt atatgtcttg
    13021 catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga
    13081 tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat
    13141 cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt
    13201 aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca
    13261 gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc
    13321 tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt
    13381 cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta
    13441 cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa
    13501 ttcgttgcag atgagatggc aaaactacat ggtggtgctg cagcacgacc tgcacaaaca
    13561 aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga tcgaacaaga tatttacgct
    13621 atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa
    13681 atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct
    13741 ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt
    13801 atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact
    13861 ttatatgtag aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt
    13921 gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac
    13981 aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc
    14041 tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac
    14101 aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct
    14161 gccaactccg cggtaataag tccagctaaa cgaaacatat gaagagctat tttttatttg
    14221 actcttctta ttacttgatg cagaacagct ttttcacaaa atttttcttc atactcaaaa
    14281 ctaatcggct ttttcacaac ctctttatac attattcctg atccaatatt tcttttaatg
    14341 ctgcttcaag agaagttaat ggttttccca atactttttc taaatcatca gaggaaatag
    14401 ctaactgatc ctttttaata tcatgttgga tcgctaaaaa catgtcagct gcttcttgag
    14461 ggaatccggc ttcttttaga ttttcgacaa acgctgcatc acttgcttca ataatttcta
    14521 actctttacc tgtcgcacgc tctaatgcct tggctaattc ttcatagtca gttagattgc
    14581 cggataattc taagatttct ggagagtcac tttctactag tacttttgct gcagcttctg
    14641 catattcacg tttcaatgcc caacctgttt ttccatttcc tgcagcataa acgaattgac
    14701 cagtttttaa agcctgccca atgataggta attcattctc tacataccaa ttattccgta
    14761 aaaatgtatg ggaaatacct gaatccttaa tcattttttc agtgaattgg tgatctggcg
    14821 ctaatacact gatcgagtga tcggcatctg caaaacttgt ataagcgata taagataccc
    14881 cagctgcttg tgctgccttc actacattct tatgttcggc ttgacgattt cctggaacgc
    14941 cagagacaaa aagcaatcga tctattcctt tcaacgcttc tttcattgag tctaaatcat
    15001 cataatcacc aatacgtata ttaaatccag cttctttcag tgcttctcct ttttcttcac
    15061 tacgtactag tacatagatt tcattttgtg gaaccatctt ttttaagaat tccagcgcat
    15121 aaccaccaaa acctccagtt gctcctgtta ctaaataatg catatttgcc actccctatt
    15181 ctttttattt gttaagattc acagctgtac atttataaat tacaccttta tcataaagag
    15241 tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac aactgatgaa gtattctctg
    15301 caatttagtg atgcaatcca tgtacttgcc tatattgaaa tgtttcaaga tacccgatca
    15361 ctttctagtg agatgattgc cagtagtatc gaaacaaatg cagcaaatgt acgtaaaatc
    15421 atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa ccggaaaacc gcagccttcc
    15481 cttgccaaaa gtccagatca gatttcttta ctagatgtct atcgcagcat cgaaggtaat
    15541 acacagctta tccaagtcga tccaaaaaca aatccaaatt gcgtcgttgg tgcgaatatc
    15601 caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa aggcagaaga agaaatggct
    15661 aagatcacac ttgatagtct gatacataaa atagccgttt tggaaaaaga aaaaagacca
    15721 gaaaacacag gcctcgtaga gagattttta taattctctc tactgtgcct gcatttttct
    15781 gatcggaatc gtctacataa attcttttat tctccggggg acaaaagcct tgtcatgccc
    15841 ctttttcctc tctaatgctc aggtcaaagc gctgaagtca tgacgtcttg ataaacggtg
    15901 ttcaagtagc tgaccctcgg cattagttct gaaaaatgca aaatatggaa agctatttcc
    15961 catttttcct ctctaatgct caggtcaaag cgctactttc acgacctcta tattatcatg
    16021 gtttattgac taccggtatc cagatttctc ccatatattt cccgttggta actcccattt
    16081 cgactacagc atttggaccc ccaacatagg cgtaatcttc tacttctctt aaaacatgcc
    16141 caaaggccgt attcgtcaaa tcatttctta attcttcagg cgattctgct aaagcgctta
    16201 caacaagata cgttcctttt gggaatggga tgattcgcgt agtgtttgga acatcctgac
    16261 tcgtttgaac accgacatag tacatcattt tcccgttata gggttcgttg actgtaaaga
    16321 aataatgatt ttcagcagct tgtttcaatg catctattgt tccattaccg ataacttttt
    16381 gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc cgtattgcta ctcttcaatt
    16441 ccacaccaat acccgtcacc gtaaaatctg gtttttcttc gatcctatac tttgccattt
    16501 ctcccaccct ttctattata ttaagttcat cttacataaa attaaaggga aatgacacga
    16561 ttacgctctt ttttctaggg atatttttat tttttaacta tttttttgct ttcactgcaa
    16621 tccaaatctc actgtaaaca ttactgaaat cctctcccca ttttgtaaaa gagatctcgg
    16681 gagcttcgac taacccataa tctgaagaag gaagccattc tgcatagatt ttcccccaag
    16741 tatcttgtaa agtttgggga aatggccctt tattaggaaa aatggcccaa gtgtgttctt
    16801 ctatagcaag catttctaaa tcagcgtacg gattttcttt tgtcgtagca aagccaatca
    16861 tgtgcatcaa actgcccttt tcttccaagc ggtctccatc aaatccataa gaaacgttga
    16921 ttacttgatt tggatataaa tctcctaact tatgcatctc ttccctttga gtctccgtga
    16981 tcgtctgcgc taacttttgt atttcttgat tgactccttc aaatttgaca ggaacttttt
    17041 tcgccacacc tgccacataa aactgttctt tcttttcgat acgaaattcc atcgattttc
    17101 ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga tttttgtatg ccgtatctac
    17161 tgatttctga aggcagatat cctgaccaat cacgaaaagc tcttgaaaaa ccttcgactg
    17221 tttcatatcc atatctaaac gccgtttctg tcactcttga tccatctaga agatcgcgat
    17281 tagccaaagc caatctacgg tttttgatat agtccattag cgacatccct gcaataaacg
    17341 taaagatgcg ttttaattga tattcagaga ttcctgtatt ttcggctatt attttatagg
    17401 aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa ttggttcaga tctttaagca
    17461 tatttatctt cctccttgat tgccttttta tcatattatc aaagaaagtc aattacttga
    17521 tgaatcagga accaatttag tcagatgtag aaaatgttgt atggatctaa agaaaattct
    17581 caagcagcgt ttcgtaacgg ataattggta ttatctgcct atctatgttt tacctttaga
    17641 aaaataaatt ttggggaaga cagattctaa gtaaacaaag cggtaaaaat atcttttatt
    17701 cccttagtta atccgcttac tacctactgt acaaagcaaa ttgttgttca aactgctgat
    17761 cagaacgctg cttggatttt tctttgcgat acatcatacc tcttttatca attggaacaa
    17821 ttatttattt ctaacaaaat cacttcattt ttgtcagata aataaaaaac aaaaaaagtt
    17881 ccatctttaa aacacgctat tcttttactt tttctcacat tctctaagaa aagaaaagga
    17941 gaatgaattt ttgacaattc gagcaaaagt gattat
//
LOCUS       DAXWLH010000049        17832 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXWLH010000049 DAXWLH010000000
VERSION     DAXWLH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.049"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            121..933
                     /locus_tag="ACGE36_001238"
     CDS             121..933
                     /locus_tag="ACGE36_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFF8383644.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLLRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            930..1730
                     /locus_tag="ACGE36_001239"
     CDS             930..1730
                     /locus_tag="ACGE36_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFF8383645.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE36_001240"
     CDS             1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE36_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFF8383646.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3227..4936
                     /locus_tag="ACGE36_001241"
     CDS             3227..4936
                     /locus_tag="ACGE36_001241"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFF8383647.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5148..9500
                     /locus_tag="ACGE36_001242"
     CDS             5148..9500
                     /locus_tag="ACGE36_001242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFF8383648.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE36_001243"
     CDS             9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE36_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFF8383649.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE36_001244"
     CDS             10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE36_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFF8383650.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11338..11631
                     /locus_tag="ACGE36_001245"
     CDS             11338..11631
                     /locus_tag="ACGE36_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFF8383651.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11628..11939
                     /locus_tag="ACGE36_001246"
     CDS             11628..11939
                     /locus_tag="ACGE36_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFF8383652.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11952..14258
                     /gene="infB"
                     /locus_tag="ACGE36_001247"
     CDS             11952..14258
                     /gene="infB"
                     /locus_tag="ACGE36_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFF8383653.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE36_001248"
     CDS             14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE36_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFF8383654.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14740..15244)
                     /locus_tag="ACGE36_001249"
                     /pseudo
     CDS             complement(14740..15244)
                     /locus_tag="ACGE36_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15409..17772
                     /locus_tag="ACGE36_001250"
     CDS             15409..17772
                     /locus_tag="ACGE36_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFF8383655.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6095 a         3153 c         3920 g         4664 t
ORIGIN      
        1 aaaaaaagaa aaaattattc agtttttaaa taaaaaaaca atgatttcat aggaaaaaca
       61 gttagatatt gctataatac ttaagatagt gggaatcttt taagtatagg aggaaaaaga
      121 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
      181 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      241 gcgcaaaacc gtcgtttgct acgagttgct ggccataaag aaggcatgga aacagtaaag
      301 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      361 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      421 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      481 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      541 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      601 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      661 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      721 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      781 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      841 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      901 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      961 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
     1021 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
     1081 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1141 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1201 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1261 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1321 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1381 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1441 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1501 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1561 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1621 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1681 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1741 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1801 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1861 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1921 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1981 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     2041 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     2101 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2161 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2221 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2281 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2341 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2401 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2461 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2521 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2581 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2641 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2701 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2761 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2821 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2881 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2941 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     3001 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     3061 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3121 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3181 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3241 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3301 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3361 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3421 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3481 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3541 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3601 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3661 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3721 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3781 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3841 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3901 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3961 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     4021 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     4081 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4141 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4201 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4261 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4321 ccactataaa aatgtgaatc caaaacgcga tttcactgtg ctttcttatg aagatcttcg
     4381 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4441 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4501 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4561 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4621 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4681 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4741 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4801 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4861 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     4921 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4981 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     5041 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     5101 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5161 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5221 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5281 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5341 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5401 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5461 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5521 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5581 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5641 tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg
     5701 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5761 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag
     5821 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5881 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5941 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     6001 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     6061 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6121 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6181 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa
     6241 aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag
     6301 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6361 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6421 acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6481 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6541 caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc
     6601 gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6661 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6721 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
     6781 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg
     6841 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6901 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6961 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     7021 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     7081 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7141 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7201 gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7261 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7321 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7381 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7441 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7501 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7561 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7621 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7681 cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg
     7741 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7801 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7861 ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7921 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7981 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     8041 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     8101 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8161 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8221 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8281 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8341 cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc
     8401 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8461 ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg
     8521 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8581 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag
     8641 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8701 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8761 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8821 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8881 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8941 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     9001 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     9061 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9121 aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc
     9181 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9241 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9301 catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa
     9361 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9421 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9481 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9541 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9601 ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga
     9661 aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt
     9721 cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg
     9781 tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc
     9841 agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg
     9901 gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt
     9961 ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga
    10021 acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa
    10081 aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa
    10141 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10201 gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc
    10261 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10321 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10381 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10441 gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10501 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10561 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10621 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10681 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10741 ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt
    10801 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10861 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10921 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10981 gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    11041 aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    11101 cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11161 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11221 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11281 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11341 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11401 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11461 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11521 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11581 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11641 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11701 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11761 ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11821 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11881 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11941 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    12001 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    12061 cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12121 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12181 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12241 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12301 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12361 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12421 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12481 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12541 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12601 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12661 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12721 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta
    12781 tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa
    12841 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12901 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12961 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    13021 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    13081 taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt
    13141 atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat
    13201 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13261 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13321 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa
    13381 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13441 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13501 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13561 gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact
    13621 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13681 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13741 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13801 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13861 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13921 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13981 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    14041 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    14101 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14161 tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14221 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14281 cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14341 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14401 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14461 agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14521 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14581 tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa
    14641 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    14701 acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag
    14761 aaaagtaaaa aattcgactt tcttttttct atagtatata aatttttgca tatagataaa
    14821 gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag tcaggtatag
    14881 ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg tcggtccgtt
    14941 gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc cgcttcgatt
    15001 tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc tgggatacca
    15061 attcacatat tgacaaagat aacagagact tttacataaa acggatcaat caggaaagga
    15121 attgctcgat ccagccccag ttttccaaca atgtgttaac tggcctagcg tcattcagca
    15181 aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata acgacaagtt
    15241 ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt
    15301 tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag gtttcaattg
    15361 ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat ggattattct
    15421 agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta tctttctgtt
    15481 gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc agaagatgtc
    15541 aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat ttatgtccat
    15601 ttgaaccgag tcatcaataa atatgatttg aacatgtttt atatcgaagg gccaggacac
    15661 ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga gatctatcca
    15721 gaagtaacgg aagatgagac aggaatgcaa aaattattca agcgattttc atttcctgga
    15781 ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg cggagagctt
    15841 ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt aatctctgct
    15901 gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg gttctcaaat
    15961 gtctttatca atccagtgat agatggagct gttttaccga tccttcattt aaatggtgca
    16021 aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc aaattacttt
    16081 aacggacttg gctgggagcc tttctttatt gaaggaaatg atccagaaaa attaaatcct
    16141 gttatggctg aaaaaatgga tcaagcaatc gagaaaatca aatccatcca aaaagaagct
    16201 cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca
    16261 ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg aaccttccga
    16321 gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga tgctttactc
    16381 cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg tattttagaa
    16441 gaaatcagag aaattgcgcc tactggtgac caaagaatgg caaaaaatcc tatcaccaat
    16501 ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac tttgcaattt
    16561 gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt cgttcgcgag
    16621 atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac aaaatcaaac
    16681 cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa
    16741 aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa
    16801 gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt tgcaagttac
    16861 gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg gatacggaaa
    16921 tcacatgacc tttcttggcg aaaagattat ccttcgctga atctcattgc aagctcaaca
    16981 gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt gacacatttg
    17041 gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa tacattgctc
    17101 gcagtcatgg ataaagcatt ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa
    17161 catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg
    17221 aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt cattgctgct
    17281 gcaggaacgg aatctaactt agaagcatta gcagctgtaa ctttgctgct ggaagaattc
    17341 ccaaaactaa aaattcggtt catcaatgtc gtagatttat tgaaattacg tcatccaagt
    17401 caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa agacaaaccg
    17461 atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt tgatcgtcat
    17521 aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac accatttgac
    17581 atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc tttaaaaatc
    17641 aaaggaagtc aagcagaaga ttttgcggaa aaaatggatc agaaattaca agaacatcaa
    17701 aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa atggaaaaat
    17761 cttgaccaat aaaatgataa aatcgatccc gcaaaacaga attttttaca ttgtattatt
    17821 tgtcaaatta ag
//
LOCUS       DAXWLH010000050        17543 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXWLH010000050 DAXWLH010000000
VERSION     DAXWLH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.050"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(116..745)
                     /gene="pyrE"
                     /locus_tag="ACGE36_001251"
     CDS             complement(116..745)
                     /gene="pyrE"
                     /locus_tag="ACGE36_001251"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFF8383656.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(752..1468)
                     /gene="pyrF"
                     /locus_tag="ACGE36_001252"
     CDS             complement(752..1468)
                     /gene="pyrF"
                     /locus_tag="ACGE36_001252"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFF8383657.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1465..2397)
                     /locus_tag="ACGE36_001253"
     CDS             complement(1465..2397)
                     /locus_tag="ACGE36_001253"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFF8383658.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2394..3170)
                     /locus_tag="ACGE36_001254"
     CDS             complement(2394..3170)
                     /locus_tag="ACGE36_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFF8383659.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3224..6409)
                     /gene="carB"
                     /locus_tag="ACGE36_001255"
     CDS             complement(3224..6409)
                     /gene="carB"
                     /locus_tag="ACGE36_001255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFF8383660.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6414..7490)
                     /locus_tag="ACGE36_001256"
     CDS             complement(6414..7490)
                     /locus_tag="ACGE36_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFF8383661.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7495..8775)
                     /locus_tag="ACGE36_001257"
     CDS             complement(7495..8775)
                     /locus_tag="ACGE36_001257"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFF8383662.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8794..9720)
                     /locus_tag="ACGE36_001258"
     CDS             complement(8794..9720)
                     /locus_tag="ACGE36_001258"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFF8383663.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9733..11016)
                     /locus_tag="ACGE36_001259"
     CDS             complement(9733..11016)
                     /locus_tag="ACGE36_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFF8383664.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11030..11569)
                     /gene="pyrR"
                     /locus_tag="ACGE36_001260"
     CDS             complement(11030..11569)
                     /gene="pyrR"
                     /locus_tag="ACGE36_001260"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFF8383665.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11804..12715)
                     /locus_tag="ACGE36_001261"
     CDS             complement(11804..12715)
                     /locus_tag="ACGE36_001261"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8383666.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12712..13176)
                     /gene="lspA"
                     /locus_tag="ACGE36_001262"
     CDS             complement(12712..13176)
                     /gene="lspA"
                     /locus_tag="ACGE36_001262"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFF8383667.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13181..13879)
                     /locus_tag="ACGE36_001263"
     CDS             complement(13181..13879)
                     /locus_tag="ACGE36_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8383668.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13866..14252)
                     /locus_tag="ACGE36_001264"
     CDS             complement(13866..14252)
                     /locus_tag="ACGE36_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383669.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14399..16069)
                     /locus_tag="ACGE36_001265"
     CDS             complement(14399..16069)
                     /locus_tag="ACGE36_001265"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8383670.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16252..16485
                     /locus_tag="ACGE36_001266"
     CDS             16252..16485
                     /locus_tag="ACGE36_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8383671.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16584..17015)
                     /locus_tag="ACGE36_001267"
     CDS             complement(16584..17015)
                     /locus_tag="ACGE36_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFF8383672.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17094..17507
                     /locus_tag="ACGE36_001268"
     CDS             17094..17507
                     /locus_tag="ACGE36_001268"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFF8383673.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         4835 a         3968 c         3118 g         5622 t
ORIGIN      
        1 tttcttttca tacctgatga aaagatgctt ctcgcaatca gtttttggat ttttgctatt
       61 ccaattgatg attctggaga cacaaatcgg atttctctat cttcatcaag cgcgcttaaa
      121 tctgccaatt ttcaggatct tttttccatt cttttagcag agttaattct tgctcatcga
      181 tataacgatc ttctaaagct gcttcaatca atacagaata attggtcaac gtcataagag
      241 ggatttcggc tttttcgaaa tttgcttttc cttttggcaa ctcatacgta aagatagccg
      301 caacaccaag gatatccgca ccttctcttt ttgctgcttc agctgcttcc aagacacttc
      361 cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg accttcaaat atacgtcctt
      421 cgatttggtt acctttccca tgatctttcg ccttacttcg aatatagacc attggcaaat
      481 ccagtatttc cgctacccat gcggcatgag ggattcctgc tgttgcagtt ccagcaatca
      541 cttgtacttc agggtaagct tctttgattt tgcttgctaa tccttttgcg atctcttttc
      601 ggacttttgg atagctcatc gtgattcggt tatcacagta aattgggctt ttgattccac
      661 ttgcccatgt aaaaggatta cttggattta aaaatactgc ttcaatttct aataagtctt
      721 ttgcgatact ttgttcaaca ttcattttag gttagctcca ttctgtttga atcgtttgat
      781 atgctttata aggattagtt gactgagtga ttggtcgacc tataacgata aaagttgagc
      841 cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg atctcctgcg actgttcctt
      901 ctggtcttat acccggagtt aagcaaacaa attcgtctcc tgccatttct ttgatcgctt
      961 ctacttccca agcagaagaa accacaccat ctaatcctgc ttttttggca cagctagcat
     1021 aatggatcac acttttttct aaagaaactt cgattagttg atcggcatgc atttcttctt
     1081 cacttgtaga agtaagctga gtgatagcta gaagtagcgg acgttttcca ccttctggag
     1141 tcccctcttc taatcctcgc attgccgcct ccatcatacg aataccgcca gcggcatgaa
     1201 cacaggtgag atcaacacca agctttgcca atccccgcat tgctttttcg accgtattcg
     1261 gaatatcgtg aagtttcaga tctaaaaata cattatgacc agcctctttt agataacgta
     1321 cgatttcagg tccttcttgg tagaacaatt ccattccgat tttcacaaac aatttttctt
     1381 ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct agttgaaaaa tctaatgcaa
     1441 taattggtcg ttggtctagt tgattcatcg ttcacgttcc tctctcactt ctttgattaa
     1501 tctttccagc gattcgatgc ctagttcgct cattcgcaca gggagctctt taattagttt
     1561 aggacagata tacggatcgg tgaaattcgc ggtccctata gctacagcac ttgctccagc
     1621 cataaacatc tcaagaacat catccactgt ctgcacgccg cccatcccaa tgattggaag
     1681 gctggatata cttgctactt ggtgaatcaa acgaatagcg acaggcttga ttgctggtcc
     1741 ggagagtcca cctgtttgat tggctaaaat cggttttctt gtcttaaggt caatccgcat
     1801 accaagaaga gtattgatca tcgtaaaccc atctgcccct ccagcttcga ttgcctgagc
     1861 gattggaacg atgtctgtta catttgggga caatttgaca tagacaggaa cagaagaaac
     1921 tttctttact gcttgtgtta attgaaaagc aacatcagaa tctgttccaa aagcaattcc
     1981 gccatgtttg acatttgggc aagaaatgtt cagctcgatt gctttcacat ttggtgcttc
     2041 gccaattttg ctacaaactt ctacatagtc ttcttcacaa gcaccagcaa catttgcgat
     2101 gatgggtaac tcattgtact cctctaattc tggaagaagt ttatgcataa caacgtctag
     2161 accaggattt tgcaaaccaa ttgcattcaa catgccagat ggtgtttccg ccactcttgg
     2221 cgtgggattg ccaaagcggg catttggagt cgttgccttg accatgatcg atccaagctg
     2281 attcaaatca tagtattttc cgtattcttt gccaaaaccg aaacagccgc ttgccggcat
     2341 gataggattt tttaattcta ggccaggaag tttgaccgct aaaggattct cattcatata
     2401 attacctctc ctattttaaa aatcggtcct tcgtcacaaa ctttcacact ttttgttcct
     2461 gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag cccctatccc acatgccatg
     2521 cgggattcta atgataactg gacattttga tgcgaagaaa acagctgttc cactgtcttc
     2581 aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt ccgtcttcgt tcctaacaat
     2641 aaattaccga catttccgtg cataccgtat gttccatcgt cagtagcgat tcttgtttct
     2701 cctagacgat taaattcttt ttcataataa acgacttcat gtgtggcaaa acctaaaaaa
     2761 tgcgttggct taattccttt ttcgactaac tgttttgata actcatatag aggagggaca
     2821 ccgattcctc caccgattat aaatacttcg tctcctgtat gaagattgga caaatcaaaa
     2881 ccattgccta aaggacccat gacatctaaa tggtctccgg cagaaagagt agagaagact
     2941 ctagtcccct caccttccac ccgataaatg atacggcaag tatttgtttc gtggtcgata
     3001 tgattcaagc taatcggtcg gcgtaatagc aagtctgcac gcggcacgag aatatgcaag
     3061 aactgtcctg gttgctgcat ttcttttact agatcacctt tcaatgtcat ttcaaaaatc
     3121 tttggtgcca gctgtttttg ctgaacgatt tccatcattt cttgtttcat ctgtcccctc
     3181 ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg ccgttaaata gggttagttg
     3241 aaaaagcacg ggattccatc actcgaacga tcgcatctgc tgtgtctaaa gaagtaaaca
     3301 atggaatacc atgttcaact gcttctcttc ggatgatgaa accgtcttta gaagcatttc
     3361 cccgatcttt atcaatcgta ttgacaacca cttgtgtttt accatctctt attaagtcga
     3421 ctacattttt ttctgctgct tcctcagaaa ttttcgctac tgtttcaacg accagaccat
     3481 ttttttcgaa ataagcagct gtatgtttcg ttcctatcag actatagcca atttctgtaa
     3541 aacgtttagc taattctagt gcttcttctt ttgtttcgtc cgcaatcgta aataatacgg
     3601 ctccatattc aggcaagtgt aatccagaag cttcgaatgc cttgtacaaa gctttgtcta
     3661 attgtctgtc agatcccatt acttctccag tcgatttcat ttcaggccct aagtaagtat
     3721 caactttctg caatttcgta aaagaaaaga caggtgcttt aacatgaact tcttttgttt
     3781 ctggataaag tccgttttca taaccaagtg tctttagttt ctctcccaaa atagctttcg
     3841 tcgcaatttg tgccattgga attcctgtca ccttacttaa gaatggaaca gtccgacttg
     3901 ctcgaggatt gacttcgatc acataaacct cattttcatg gatgacaaac tggatattca
     3961 tcatcccgac acaattcaaa cctaatgcta agcggatcgt gtaatcttcg atcgttttct
     4021 tgatcgattc agataatgtt tgaggtggat acacagccat tgaatcccct gaatggacac
     4081 cagcacgctc aatatgctcc atgatccctg ggatgagtac tgtttcaccg tcacaaatcg
     4141 catccacttc gcattcacca ccaagcaagt aacggtccac tagtactgga tgttctggag
     4201 atgctttcac agcatgttcc atgtaatctt ctaaatctcg ctggttttca acgatttcca
     4261 tcgcacgtcc gcctaataca tagcttggac gaacaagaac tggataacca attctcgaag
     4321 cgataaccac ggcttcttct ttacttgtag cagtatcccc taaaggttga ggaatatcca
     4381 actctttcag cgcttgctcg aataaatcac gattttccgc acgatccaag tcttcgatcg
     4441 ttgtccctaa gatttttaca ccagcagcag tcaaaggttc ggctaagttg attgctgtct
     4501 gaccaccaaa ttgaacgatt actcctatcg gctgttctag ctcgatcaca ttcatcacat
     4561 cttcgaaggt caatggctca aaatataatt tatccgaaat agaaaagtca gtcgaaactg
     4621 tttcaggatt gctgttcatg atgattgctt cgtatcctgc ctgctggatt gctttgacag
     4681 aatgcacagt cgcatagtca aattcaaccc cttgtccgat acggatagga ccagatccca
     4741 gtactagaat tgagggcttt tctgatcgtg aactttcatt ttcatattca taggaactat
     4801 aaaaataagg tgtttttgat tcgaattccg ctgcacaagt gtcaaccatc ttatatacag
     4861 gaatcaaatc attttctttt ctcatctggc gaacggcttc ttcgtttatt tcccacagct
     4921 ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt tttcaatatc ttgtcatcca
     4981 acggctgctg tttcaactgc tgttcgattt ctacgatatg caacagttta tctaaaaaga
     5041 ataaatcgat ttttgtcaaa tccgctaatt cttctatggt gtaccctctt ctaatagctt
     5101 cagctacata aaacaggcga tcatcttgtg ctcgaaccac tttttggatt aggtcatcgt
     5161 ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt accgatttct aaagaacgca
     5221 cagctttcaa taaagactct tcgatattcc gaccgatagc catcacttca ccagttgctt
     5281 tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt atcaaacggc cagcgaggaa
     5341 ttttagctac aacataatct aatgcaggtt caaattctgc aaaagtcgtt cctgtaacag
     5401 gatttttcat ttcgtctaat gttaatccaa gcgctatttt agcagctagc ttagcgatcg
     5461 gatagccagt cgccttactt gctaacgcag atgaacgaga aacacgcgga ttgacttcaa
     5521 tcacataata attaaaacta tgtggatcaa gagcgagttg gacattacag cctccctcga
     5581 tcttaagtgc tcgaataatc gaaagagaag catcacgaag catttgatat tcatggtcag
     5641 acaatgtttg actaggggca aatacgatag aatcacctgt atggataccc accgggtcaa
     5701 aattttccat attgcagaca acgattgcat tatcagccga atctctcatc acttcatatt
     5761 caatttcttt aaaacctgca atacttcgtt caatcaaaca ttgggtagcg ggggaaagac
     5821 ttaatccatt ttctgatacg atacgtaatt cttcttcatt ttcacacata cctccaccag
     5881 ttccgcctaa agtaaaggct ggacgaacga tgacaggata gccgatttta tttgcaaaat
     5941 ctacagcttc ttctactgta ttgacgattt cagactcggg gattggctga ttcaattctt
     6001 tcatcaactg tttgaataga tcacgatctt ctgcttgatc gatcgcagaa agctttgttc
     6061 ctagaagttc tatgttcaat tcttctaaaa tacctgatgc tgccagctcc attgccatat
     6121 tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc tggacgctct tttcgaagaa
     6181 tccttgatac aaattcaagt gtgatcggtt cgatatatac ttgatctgcc acttctttat
     6241 ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac ttcatatcct tcttctttta
     6301 aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc ggcttgacca atgatgatcg
     6361 gaccagatcc aatcaccata attttcttga tatctgtacg tttcggcatc tgtttactgc
     6421 tcctttcctg cgtccatcat ttccataaat tcatcaaata aatgtagtgc atcatgcgga
     6481 ccaggggcag catctggatg aaactgcaca ctgaaagcgg gataatctct gtgtttgact
     6541 ccttctactg ttccgtcatt tacttctaca tgagttacga tgagtttttc tggatcaatc
     6601 gtggcttcat cgactgcata gccatgattt tgagaagtaa aatcaattct tccggtcgct
     6661 acttctcgta ccgggtgatt taatcctcga tgtccgaatt tcattttata ggtatctgca
     6721 ccgttggcta aagcaaataa ctggtgaccg agacaaatac caaaaatcgg tacttttcct
     6781 tggatttttt tgatcatttc gattgcttct ggcacatctt ttggatcacc aggtccattc
     6841 gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct cggcatctgt gttataggga
     6901 agtactgtga ggttacagtt tcttttactc aattctcgta aaatactatg tttcaagccg
     6961 aagtcaatga caatgacttt ccgacctgtt cctggacttg gataaggttt gttagttgag
     7021 acttgctgca cttgattggt tgctagaacg gttgctttca gctgatcaaa tgcatgttcg
     7081 aaggaatctc cagcatcaac gatactagct ttcatcgttc cgacttcacg gattttgcga
     7141 gtaagggcgc gcgtatcgat acctgatatt ccaggaattc ctttttgttt caaaaatgaa
     7201 tctagcgtca tttggtttcg ccagtttgaa gctacacgtg catgttcttt tactactact
     7261 cctttgcatg ttggagaaat cgattcataa tcatctctat ttaccccata attacccacc
     7321 ataggatagg taaatgtaat gatttgaccg ttaaaacttt gatcagtgat agtttcttga
     7381 taaccagtca tacttgttgt aaaaacgatt tctccgaaga cactagcatc tgcaccaaaa
     7441 gcctttcctt cgaaaactgt tccatcttct aatataagca aacgttccat acttttattc
     7501 tcccttctgc cacacaagtt ttccgttgac aaaagtcatt tgtgtttcgc cttttacttt
     7561 ccagccgata aaaggtgtat tctccccttt tgataaaaag tcttctttat cgatcgtgca
     7621 agtttgtgta atatcaaaaa ctgctacatc agcgggggca ccaaccgtta gcgttcctgc
     7681 attcaaacca aaaatctcag ccggtttaac tgccaaccaa ttgatcactt gttccaacgt
     7741 gaatcgacca gtttctacaa aatgagtata gatcagttga aacgctgttt cgcttcctac
     7801 gataccaaat ggtgatttca tgaaactttg gcttttttct tctaatccat ggggggcatg
     7861 gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct tcgatcaaag cttctctgtc
     7921 ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg aaatcttcgg gaatatcttc
     7981 gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt acgtggattc ctgctttttt
     8041 tgcgtctctg attacacgga cactttcttt agtagaaaca tggcaaacat ggtaatgaac
     8101 accagttgct tcagctagca acagatctct agcaatttga gaagactcgg ttacgcttaa
     8161 gatcccggga agaccaagtt cttctgcctt cttgccagcg tgcataacgc cgccaaataa
     8221 taaagattca tcttctgtat gggctactag ggctttattg ttttttgcag cttctttcat
     8281 agccagatac atcgtcccag ctgtttggac acctacaccg tcatttgtga aagcaaaagc
     8341 accttcttca atcagtgctt cttgatcaac taatttctca cttcgcagat tttctgtgat
     8401 tggcgcatac tgcaatacct tcacaactgc atcttttcta atgatgtcat atacttgtct
     8461 cagcttttct gccgtatcag gtacaggatt taaattcggc atcgcgcata cagtagtgaa
     8521 accgccacga gcagctgagc gagtcccagc ttcgatcgtc tccttgtatg taaatcctgg
     8581 ctctctcaaa tgaacatgga cgtccactaa accaggtgtg atcaattgtc cctttgcatc
     8641 aaacacttca tcaaattcgg cttctgagaa tcctgtaccg attgctttga ttcgcccatc
     8701 ttctatccaa atttctgctg gtgtcgtcat attttttcgt gtattgatct gaccgttttt
     8761 aatgagtgtt ttcatttttt catcctccta taattacgct tttccattta gcactgcttc
     8821 taagattgcc attctgacga atactccatt gctcatttgc tgaacgatcc gcgactgtaa
     8881 tccttccact aatgaatcag ctaattctac atcacggtta actggtgctg ggtgcataat
     8941 gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt aacccatgtt cctgatggta
     9001 gccttctttt gagaaacttt cttttccatc atggcgttcg tgttgcaccc gaagcatcat
     9061 catgacatca acttgatcta gtagttcatc caatggcata taatgtccgt acacttcgaa
     9121 ttcatcgtcg taccattctc taggacctga gaagaatact tgtgcgccta gacgttttaa
     9181 catctgcata ttggatttag ctacgcgaga gtgagtgata tcacctacga tggcaacttt
     9241 cagaccttca aaatgaccaa attcttcata aatggtcatc aaatcaagca agcattgggt
     9301 tggatgctgt ccgcttccgt ctcctccatt gataatcgaa cactggattg ttttactttg
     9361 tatcagttcg tcataatagt tctcatcacc atgacggata accgctacgt ctacgcctaa
     9421 tgctgacatc gtcagtactg tatcgtatag tgtttcccct ttttggacag agcttgtgct
     9481 tgcttcaaat tcaatcactt ctagccctag ttttttctcc gctacatcga agcttttatg
     9541 tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag tactggcgct tttgcggagt
     9601 ccaattagca ccttgcttga attcctgccc tctgcggatc aagcccataa cttcttgatc
     9661 agtaagtgcc tcaacggtca ataaatgctt taagctgata cgttctgatt tgatgatcat
     9721 aatgtttcct ccttagtttt cttcgcttcg tgaagtttct ggtaagataa gatttaaaat
     9781 gatgccaagt acagttgcta atgccattgc cgacaatgta aatgtgccta cttcaaagac
     9841 gagtccgcca attcctacta ctaagataac tgaagcaatc aacagatttt tctttttatc
     9901 aaaatcgatt ttgttctcga ttaaaatctt cattccactt gcggcaatta ctccaaataa
     9961 gataaaactg attcctgaga tgactggtcc agggatactt aaaatcaaag cgcttaattt
    10021 tcctacgaat cctaaaatga ctgcaaatac tgcagctcca ccgatcacaa acacgctatg
    10081 gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa cttgttacag gaggacctcc
    10141 gacaaaggct gccacgattt gtgctaatcc atcacctgtc aacgtcttat gaagacccgg
    10201 ttcttcaaag aaattacgtt tagttagttt attcaatacc ataagatggc cgatatgctc
    10261 tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt gctccaagat atagctttgg
    10321 ttcatattgt acaaagggaa tctcaaagtt aggaacagca aaccaggcag cgtctacgat
    10381 tggcttagta tccacgattc caaacagcaa ggctacgatg tatccgctaa taatacctag
    10441 taaaatcgga atcaatccga gaaaacctct tagccacatg ttgaagaaaa ttgttaatcc
    10501 taatgtgatc agcgctacag caatatactt gaaatcatac gtgccctgat tatacatagc
    10561 atttgttgct gcatttgccg caagtcccaa cccgataacc atgatgactg gaccgacgac
    10621 gattggaggc aagattttat ccagccatgc tgaaccgatc tttttgatga tcagcgcaac
    10681 aatcaaatag actaatccag ttgtgactgc tccttgtgca atcgctggat aaccatctgt
    10741 tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg cttcctaagt acgcaggtat
    10801 ctttccttta gtagtaaata agtacaccaa tgtacctagt cctgaactga ctagcgcaat
    10861 acctggatcg atacctacta agatggggac taacacggtt gctccgaaca tcgtgaacaa
    10921 gtgttgtaaa cttaatccta cccaatgtcc tggggttggg cgatcgtgga tatctaacac
    10981 cacgtcatca ttatgaaatc cttttttctc cgacatgttt ctctctcctc taatcttcct
    11041 ctttcaaaat catgatgcga tcttgtccat caagctcttg catctctact aaaatctctt
    11101 ccgcccgaga tgttggaatg ttcttcccaa cataatctgc acggattggc aattctcgat
    11161 gcccacgatc gaccaggact gctaatgaaa tttttcttgg tctgccaaaa tccattactg
    11221 cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac atcatcaatc aaaataactt
    11281 cttttccttc taaggaaact gggatgtcag aagaatgaag ttccggttct tctggatttt
    11341 cttttttgtc atctcggtat aaagtgatat ccagttctcc tacaggaata tcgatatctt
    11401 ctagctgctt caggcgttcg gcaattcttg atgcaatgta gattcctcgt gttttgatac
    11461 ctaccaatac gatatcttgg atactatggt ttctctcgat aatctcataa gtaattcgag
    11521 tcaatgctcg cttcatggtt acctgatcta ctacttcttt tgcttgcatt ttcattcccc
    11581 ctactttttt gcataaaaaa actcctgccc taagaggaca ggaggaatgt ttagactgag
    11641 cgtatactga tacactgata gctatactat gtcaatgagc aatctactta tggcgcacca
    11701 tacgtatcct tgctcccacc cttcttagcc tcacgggact aaacttaaag gaattctatt
    11761 aagttttatg ctttcgttgt taattactat accgttatct aaattaaaat gcaatgtttt
    11821 ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag aggtgcttca aagatcatct
    11881 tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc atggagaaac tgaccattgc
    11941 ctttcaaagt tttcttcggt ccgtatactg gatctccagc taacggataa ccgatatact
    12001 tcatatgcac acggatctgg tgtgttcttc cggtttctag ttttaattca accagagtaa
    12061 atccttcgaa acgttccaat acagtaaaat gtgtgacagc cgacttccca tcttctctta
    12121 tcgcctgcat tttccggttt tccttcgaac ggccgattgg cgcattgatg gttccttttt
    12181 cgtgaggaat ctctccatgg actaaagcaa catatttgcg caaagaggtc ttatctttta
    12241 attgctgagc taatgattcg tgtgcctggt catttttggc aaccattagc agtccggaag
    12301 tatctttgtc gattcggtga acaatacctg gacgaataac gccgttgatc gtagaaagat
    12361 ccttgatatg gtacatcagc gcattgacca ttgtgccatg cgtatgacct gctgaaggat
    12421 gaacgaccat tccttgaggt ttgtttatca ccgctacatc ttcatcttcg taaacgatcg
    12481 tcaacgggat attttctggg accacatcta atgtttctgg ttcggggatc aagacagtaa
    12541 tctcgtctcc ttgttttact ttgtaatttg ctttgactgg ctgtccattg acagaaaccg
    12601 ctccattttt cagccacagc tgaatttgcg aacgactgta atcggtgaat aaattagtca
    12661 atactttatc gatcctacca gattcatggt gaatcgtcgt tttaagttct gtcatttttt
    12721 tctttgatcc ttttcatcta aaataatata aattaacata caaatcactc cacatacaag
    12781 tgtcacatct gctacgttaa aaatcggaaa gctcataaaa tctgtctgga acatatctac
    12841 tacatagcca agacgaatac ggtcgataaa attgccaatc gcaccagcta gaatcaaact
    12901 caatccaatc atgaaccagc gattaccttt ttcacgattt ttaatcatca atgtgagaat
    12961 cacaacgact gcaatgagtg tcactatagt aaagaaccac atcttccctt ctagcatact
    13021 ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa atacctggta taagattttt
    13081 cgtctctcca agcgctagat tatcagtgat ccaccatttg accaactgat ccactcctac
    13141 tagcaaagcg ctgaatatcc aataaataat taacaaaaaa tcaatccttt tctatttgtt
    13201 tataaatatc acgaggtcgt acgatcccga gcagattacc atcgggattc ccatctttag
    13261 tgataagtac ggcttccaaa gtcccttgct cacgaaacat cgcttcaacc tcgtaaatat
    13321 gtgttttagc tggaacaaaa tgataatttg tgtactttcc gttttggatg atcaaatcca
    13381 acgctcgttt atttgttaaa tcaaccggac ctttttggct ttcttttgct agccaaaacc
    13441 cgatattacg taaagtaatc aatgctacaa attttccttt attgtataac ggaaattgtg
    13501 aatacctttt ttctgctact atctttaaca gttcaagtaa cggaagatct tgttcaaaac
    13561 ctgttacacg ttttgcaaat cgaggcagca cggtttcagg ctgaatcagt tcttgttcga
    13621 tcagctggat tcgcttgact gtccaatcat ttggctctgc aatgacaaaa tcgactgcga
    13681 tccgatcatg gacgatcgca ttccttaact gagcaagctg taaaagatca tcttcgtatt
    13741 ttctgatcat taattgtttt ctttgagcta ggcgtctgac aagttcggta aatcccatat
    13801 ttcgtgcatt tcccatctcg tctctcatcc atttttcgat tcgattgaaa ctgattaaaa
    13861 aaagatcaga gtttgccata attttcctcc ttgatcaaat atgcccggtt ttttatatag
    13921 aaaaagaacg caatcaacac aataactgca aaaaaactga aaaataaatt ttgtaggaaa
    13981 acggccccca acccagttgc tagaaataaa aacggataga tttgaagaat caagtaattc
    14041 attgtgatca cataagcagg ctttgcttct ttcactgtat aaacggggct ttttagccaa
    14101 taaaaggtga cctgcatcgt aaagggcact tccagatcaa gcgttatctt ttgttcatga
    14161 ctgagaacac tgcgtttttc attgttgatt cttagctgaa tcgagctacc cataccgtaa
    14221 aaaccggttt ttcttataaa tgtcgttttc atcataccct cctattatgc tttagtatag
    14281 ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa caaaattaat tccctatcgt
    14341 ctaaaaaaag aagctgtagt caaaatacag ctccttcgat ggtagattat aatcaagctt
    14401 aaaacaatcc caacacttct ccgtcatttg tcacgtccat attcaatgca gcaggttttt
    14461 taggcaaacc aggcatcgtc ataacatctc cagtcaaggc tactaaaaat cctgccccta
    14521 attttgggat gatttcgcgg atcgtgatgg tgaaatcttc cggtgcacct agcagtgaag
    14581 gatcatcaga aaatgaatac tgtgtttttg ccatacaaat cggcagtttg tcccatccat
    14641 tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct gaaaacaaca ccatttcctc
    14701 cgtaaatttt cttgacgatt gtttctgtct tttcttgtat cgtttcttct aggcggtata
    14761 atggcttgta attagcttct tttctatcaa tcaatcgtac tacagctttt gctaaatcaa
    14821 cgccgccttc tgctccatct gcccatacac tcgctctctt ggcaagaatc ccctgatttt
    14881 cacacaagtg ctccaacagt gtgagttcat tgtctgtatc tgttacgaat tcattgattg
    14941 cgacgatcac aggtagctga tattgttcca tattacgtat atggcgcttt aaatttgcaa
    15001 aacctacttt caaagcatca agattctcat ttttaagttc atcttttttc attccgccat
    15061 gcattttcaa tgcacggaca gtggcgacaa tcaccacagc atccggcgct tttttcaagt
    15121 taggtacttt gatatctaaa aatttctctc cgccaagatc tgcaccaaat cctgcctctg
    15181 tgacaacgta atctgctaat ctcaatgctg tcttggtagc taaaatacta ttgcagccat
    15241 gagcgatatt ggcaaacggt cccccgtgga caaaagctgg tgttccgtaa attgtttgta
    15301 caagattcgg cttgattgca tctttcagca gtaatgccag agcgccttct gctttcaagt
    15361 ctccagcagt cacaggctga cggtcaaatg tatagccgac aacgatcctt gcaagacgag
    15421 cctttaaatc ttcaaggtct gccgcaagac acaagatagc catgatttcg ctggcaactg
    15481 tgatatcaaa cccatcttct cttggaacac cttggattgg cccacccaag ccaacaatta
    15541 ctttacgcag ttcacgatca ttgagatcga ctacccgttt ccaaatcact cggcgggcat
    15601 cgatgttcaa ttcatttcct tgatgaatat gattatctaa aagagcagat aatgcattat
    15661 tagctgttgt gatagcatgc atgtcaccag taaaatgcaa gttgatatct tccataggca
    15721 atacctgtgc atatcctccg cctgcagcac cacctttgat tcccatcaca ggaccaagtg
    15781 agggttctcg caatgcaata acagattttt tattgatttt attgagcgcg tctcctaacc
    15841 ctacagtgat cgttgacttt ccttcgccag ctggtgttgg attgatggaa gtaactaaga
    15901 tcaaatgccc ctcagcgtta tcctttgatc gattgattcc agaaaaattg attttcgcct
    15961 tatatttccc atacagttcc aaatcgtctt ccttaagatc taatttacta gccacttcaa
    16021 caatcggttc tagctctgtt tcttgtgaaa tttgcagatc attcttcatt tgattacccc
    16081 ttttcacgaa ctttattcaa caaaatatct tctttgttaa gatatgccaa ttataccttt
    16141 ttttattcag aaaaaaaaca taaaattaca ggtttttaat ttatttgacg tacttataaa
    16201 tactctttat aatgaaggct atgatcagac tttagaaagg tcgtgagtgc tttgaccaca
    16261 ggaattgtaa aatggtttga caacaaaaaa ggatacggct ttatcagtta tgatgacaca
    16321 gaagaaattt ttgtacattt tactgcgatt gaagaagagg gattcaaaac tttggaagaa
    16381 aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag gaacacaagc tgcccatgtc
    16441 aagaaaggca acgttcagac taccgatact agtatgcatg aatagataaa caaatagagt
    16501 gagacgctaa acgggatgaa ccgtacgtct cactcttttc ttttcctgta aaaattcgtt
    16561 tttttaggtt ctgattttta aaattagctt tttctttttt tcgtataagg ataaggaatt
    16621 gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa ggactacttg atcttttttt
    16681 cgcatcatga aaacagatgg ggctttctca tcttgcaaca tcatttgcac cccagtatta
    16741 gttggcgtca aagatcgaat ctgtccgaca gatatttctc ggtctttcca aaaagcagca
    16801 taacgtataa tcacagagtt tgattttaaa ataaaagaac gccgcaaacc tagaaataga
    16861 aaaacaagaa aaatacctaa aacaaaattg cttttccaat aaggacctgt gttttctaaa
    16921 gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg accaatagat gatcgtatgt
    16981 gccagttcag gctgccatcg aatatttttt ttcattctct aacctctcac cgattcagta
    17041 atgatataat cttaccataa cattagtttg aacaaaaagg agaatctctt tttatgttaa
    17101 aagtatacat tgatgcctct acaaaaggca accctggtcc tagtggaggt ggaatcttga
    17161 ttgtctatca aggtaaacaa gaacagctat ctatcccgct ttctataggg acaaaccatc
    17221 aagcagagtt cgaagtcttt ttaaaaactt tagaatttct aaagaaaaac gacttacaga
    17281 atgaaacgat tttctgtttt tcagacagca aaacactagt ttctacggtc gacaaaaacg
    17341 caacgaagaa cgagctcttc cttccctacc ttcaaagaat ccaagaactt ctatccgaat
    17401 tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa taaaggagca gataatcttg
    17461 cacgtcaggc tctccataaa caattaaaag aaaaaaagac tctttaaatt aaaaacttta
    17521 aggagtcttt ttatagtttc tca
//
LOCUS       DAXWLH010000051        17424 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXWLH010000051 DAXWLH010000000
VERSION     DAXWLH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.051"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(275..1057)
                     /locus_tag="ACGE36_001269"
                     /pseudo
     CDS             complement(275..1057)
                     /locus_tag="ACGE36_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1122..1958)
                     /locus_tag="ACGE36_001270"
     CDS             complement(1122..1958)
                     /locus_tag="ACGE36_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8383674.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1976..3385)
                     /locus_tag="ACGE36_001271"
     CDS             complement(1976..3385)
                     /locus_tag="ACGE36_001271"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8383675.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3399..5222)
                     /locus_tag="ACGE36_001272"
     CDS             complement(3399..5222)
                     /locus_tag="ACGE36_001272"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8383676.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6065..6391)
                     /locus_tag="ACGE36_001273"
     CDS             complement(6065..6391)
                     /locus_tag="ACGE36_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383677.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6437..6799)
                     /locus_tag="ACGE36_001274"
     CDS             complement(6437..6799)
                     /locus_tag="ACGE36_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFF8383678.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6836..7051)
                     /locus_tag="ACGE36_001275"
     CDS             complement(6836..7051)
                     /locus_tag="ACGE36_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8383679.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7178..7513
                     /locus_tag="ACGE36_001276"
     CDS             7178..7513
                     /locus_tag="ACGE36_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8383680.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7589..9586)
                     /locus_tag="ACGE36_001277"
     CDS             complement(7589..9586)
                     /locus_tag="ACGE36_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383681.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9684..9959)
                     /locus_tag="ACGE36_001278"
     CDS             complement(9684..9959)
                     /locus_tag="ACGE36_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383682.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10191..10925
                     /locus_tag="ACGE36_001279"
     CDS             10191..10925
                     /locus_tag="ACGE36_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8383683.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10964..12106
                     /locus_tag="ACGE36_001280"
     CDS             10964..12106
                     /locus_tag="ACGE36_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8383684.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12201..13592)
                     /gene="sufB"
                     /locus_tag="ACGE36_001281"
     CDS             complement(12201..13592)
                     /gene="sufB"
                     /locus_tag="ACGE36_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFF8383685.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13597..14067)
                     /gene="sufU"
                     /locus_tag="ACGE36_001282"
     CDS             complement(13597..14067)
                     /gene="sufU"
                     /locus_tag="ACGE36_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFF8383686.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14054..15289)
                     /locus_tag="ACGE36_001283"
     CDS             complement(14054..15289)
                     /locus_tag="ACGE36_001283"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFF8383687.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15286..16572)
                     /gene="sufD"
                     /locus_tag="ACGE36_001284"
     CDS             complement(15286..16572)
                     /gene="sufD"
                     /locus_tag="ACGE36_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFF8383688.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16585..17358)
                     /gene="sufC"
                     /locus_tag="ACGE36_001285"
     CDS             complement(16585..17358)
                     /gene="sufC"
                     /locus_tag="ACGE36_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFF8383689.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         5226 a         3613 c         2910 g         5675 t
ORIGIN      
        1 ttttcataaa aaaacagtac tgtctagtag tataaaatat acaaatttct taaaaatttt
       61 ttcactaaaa tcactacctg aaacagtcta aaacacttat atatcaacgt ttaatgaaac
      121 tatctccgtt tcatttcaga tacaaataaa tatagtcggg ctccaaatcc gaaaaaaaat
      181 acccccagta gcttatccta agctactgga gatgtataag tacggtcata taacaacggg
      241 gtagggtaaa taaatgaaaa agatggaaga atcattattc aataactttt ttaaatactt
      301 cgttgtaatc agttacctgt ccgttcttaa ataccacaga ataatctttt tacccatttt
      361 caagtaaccg aagtggaatg tctttaccaa cttgctctgt cactgcatta ctgatacctg
      421 ttagattagc taataaatct gataaagatt gagcatctgt agaaatctct gtatcagtat
      481 ttatcgtgat tcctttatct tcatttacaa gcttccaagt aggtggtata gctgtgtatg
      541 tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc agtatctact tctgtctcat
      601 tactcttaga tgaatcgatt ctttcatcca tcgtctgctc cgtaactgct tctgtctcat
      661 tattctcacc ggaaactctc ttcacaccca aacttatata ttgtccatct ttagtgtgca
      721 tagttaaaat tcccacgctt ttactaataa attcactata accagttctt tcaatagaga
      781 cttcaatgta tttcacacta tcatactcaa aagaaaaata gacatgtaaa agcattttgt
      841 tgtttacatc tacataataa tcctttgact ctacttgaga ccttcctata cggaatacag
      901 ctttactgtc tgatatctct ataccagcat ccttattatt aatctgccaa tctccaagta
      961 gctcttctgg caagctagta gtttcttgtg aacactttac cacttcactg gtagaggtcg
     1021 aaacattcac ctccgcactg ttagaagaat tttccaagga atcaggagcc ttagcacaac
     1081 caacaagtct ttatatattg tgcagccgat attttcttca ttcagtacac ccttgaaata
     1141 tgcagtgtta gataaagcag ttcgtcctga tttgctgagg cacccatctc ttcttgaacg
     1201 tactccttga ttttcaaggc cgcccgatac tctttcggaa aacgctgctt cactgaagcc
     1261 agcaaaaaat catccttctc cggcaactct tccttcttaa tcattcgggc aataaaaaac
     1321 tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt tatcaacctt tttttcggga
     1381 aacagatatc ctagcatagc caagatatcc tttagtgctc ttaccgagag catggtttct
     1441 tccatatttg cttgttcact ttgggcattt acaatgtgaa acgcaatgtt tccagcctcc
     1501 tcttctggca actgaacatt catttttttg ttgatcaatt ggatagcata gcagccgatc
     1561 tcaaattctt ttgggtaata cttttgaatt tcaatcagca ggcgattttg aaaaacaatg
     1621 cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa ccaaggtaaa aaatagactt
     1681 ttattcagct taaagtcaaa cttggcgttg gcataatcga taattcgctt gacgcaaata
     1741 attagatcat ccggagcagc ctcaaacagt ttaatgtaat ccttttttag agcatcagaa
     1801 agtgaatcta atttaaatac tttttggatt ttgtcagccg gaaccaaatc gcctttcttt
     1861 agttgaaatc caatcccttt ccccatcaaa attacttctt tgccttgctt atctaagcaa
     1921 agaattaaac tgttgtttaa cgttttaatt actctcatta gattcaccgt tctctctaag
     1981 ctaagccatt tcttgcgatt actttttgat accaaaagaa gctttctttt tttattcgtt
     2041 ttaattcttt aagattctca tcggttcggt caacatatat aaaaccataa cgcttattga
     2101 agccttgatg ggtactaaca acatcaatcg ctgaccaagg taaataacca aaaacatcta
     2161 caccatctgt taaagctgct tgaatagcca gaatatgttt ggctagataa tcaattcgat
     2221 cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg tgccccatag ccattttcag
     2281 taattaaaat aggtaaatca tagcgatcgt acaatttcct caatgttaat cgtagtccag
     2341 tagggtcaac tacccagcca tatggcgttt tatctacata aggattttca gctgcccggt
     2401 agacaccttc ttctcccaac atgatctgtt gatcccccgc tctagcggaa acatctgatc
     2461 cgtcattttt gctggcagcg atggtggcag ttgaataata attaatcgca ataaaatcgg
     2521 gtttaccgga ggataaaatt gcctgatcct ctgcctcaat tttaggatat aatccacgat
     2581 cattcaaata gttttcaaac aaataattgt attttccccg aactgctaca tctaagaaag
     2641 accagcctct caatgtttcc caattatggg ctgcaattgc atcttctgga ctggaggtag
     2701 cttgatacat agccgttaaa ttgattgctg gaccgatttt tcctttaaga ttcaactgat
     2761 gatacaagtg aattactcta gcttgagcta ataaaacatg gtgattaatt tgaaagagtt
     2821 ctttttttga aggcagctct ccacttggtg gcaaaccaat cgctccagga tgaaggatca
     2881 tcgtgttttg ctcattaatt gtcagccaat agttcacctt gtcaccatac tcagtaagca
     2941 atacctgtac atagtccaca aaataatcga tagttgctcg atttaaccaa ccgccctgct
     3001 cctctagaaa ataaggtaaa tcgaaatggt acatggtgac aatcggttcg attccctgat
     3061 tcaggcattc atcaattact tgatgataaa aagaaattcc cttttgattg gttttattat
     3121 tttcaatatc cataattcgc gtccaagcga tggaaaagcg aaaagctttt aagcctaatt
     3181 ctttaaacag ctgtatatct tctttatagt gatggtaaaa atccgaggca acttcaaaat
     3241 tagctacccc ttctgtatgc tgatacttgt caataataga cggttgtttc ccatcctcct
     3301 gataggcacc ctctgcctgg taggcactaa tggaggcacc ccataaaaac ttctctggaa
     3361 atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt aactaatttg catcaatggt
     3421 tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg cgtaattttc actgttggtc
     3481 actaccacaa tcactgtatc atcgtagcca gcgtttctaa tggcagcgaa atcggcgttg
     3541 attaataatt gtcctgctga cacctgatct cctgctttta caaatacttc aaagggttcc
     3601 ccttttaaat tcacggtgtc gatccccaca tggatcaata attcaatacc ttctttagaa
     3661 cgtagaccaa tcgcatgttt agattctaaa atagtaatga cttctccctc aacaggtgaa
     3721 taaatgctgt tttcttctgg atgaatggct acgccctttc ccaatagtcc cttagaaaag
     3781 acagcatcgt tcacttcaga aataggacac acggttcctt ttaccggatt ttcaacaatg
     3841 actgcttcgg ctattgccgt ttgctctgat tcatcagaag tatcgtcaaa tccaattaga
     3901 taagcaatga taaaggttgc aacaaaggcg attacacacg taataattgc gtgaacaata
     3961 ttcattggat tttctccaat aaaggctggt aaggctgcta agccagggga aacaaaggca
     4021 tatctgacta aaccgctcaa gccagcatac aaaccagcaa ttgcaccacc aatcatggaa
     4081 gcaactaatg gttttttcag cttcaaggtt actccataga gtgaaggctc agtaattccc
     4141 attaaagcag tgatcccagt ggacacagct agctgcttca actctttgtt tttagttttc
     4201 gcggctacag caaaggtagc acctgattga gcaatattag aagccagcat tcctggccca
     4261 ttgatcattt cagaacccgt actgctcaat tgcatagtag cgattggcgt cattgcccaa
     4321 gctgtacccg taaccaccaa aaatggctgc aaaccaccca tcaaaaatgg aattaaccaa
     4381 gctgctttgc cgttaatcca ttcagccccc attgctataa gatcgttcag caacgttcca
     4441 aacggtccaa tgacaaccaa cgctaaaggc cccataatta aaaataccag catcggctta
     4501 gtaaagaatt taataaacga aggcgaaatt cgatccgcaa agggctctac ataggacatg
     4561 atccaaacag taaagataat cggtaaaact gatcccgcat aatctgcagc taataccgga
     4621 attcctagaa aagaaacggc atcgccagta gaaaaaagct gtgaaatatt tggatgaagc
     4681 aaaatgccag caacagtaat cgccaaaatt gggttgacat caaactttat ggctgcccca
     4741 taggctaata gtactggcat aaaataaaag gctgcatcag caatagtatt caaaatgatg
     4801 taagtttgac tactgtctga tagtacacca gtcaaagtca agacggccaa aatggctttt
     4861 accatcccgg cacctgttat agctggaatc atcggtgtaa aggtggtaga aatgacacta
     4921 atcaatcttg aaataatact ttcttgattg tctgaagagg ggcccttttt aattgaagtt
     4981 cccacttcct tttgaatttc cttgaaggct gtttggactt cattgccaat caccacttgg
     5041 tattgtccac cgctatccac cacactaatt acaccaagtg ttttttctaa ggctttcgta
     5101 tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg tggcacaatg ggttaagctg
     5161 accacatttt cttttccccc aacctgttgc aatacatttc ttcctacttc tctgtaatcc
     5221 attattctct ctccttaaag cagtaaaaaa gaccaagtca aaccacgccc caatcaacat
     5281 tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt aacatgccaa taacgttatt
     5341 ttgctgtaat ccaataatag cacattatga tagcgcaatc aacagggaat ttaatttttt
     5401 atcttttcgt ttgaccgtat agggtaatat aacagttaga tcacatcaac tagggtatat
     5461 ctgcgtatac ttactgctaa aataagtata tctaatatta actataccag taaaaccatt
     5521 cctacataac cagcgggaaa ttcccaaaat cgtgaaagca tcaaaaatgc atataacgga
     5581 tcacacaact tcaacaattc tgctcttggg aaaaattgat gaatttggtg agtaaaggat
     5641 ggaataaaca gcattccaac aatataggca acaatagtga tacttgtttt tttggtaaaa
     5701 atcagtaagc aatagccaat ttcaatcatt ccgagatata aaggtatcaa tagaatgatt
     5761 tgggctagta ttcttaaaaa ttaactagca aatgtttaaa ctgatttatt tctaatctac
     5821 atgtagtaag tatttcaatt ccaccaatta tcaataaaaa taccaaacat atccataata
     5881 attttttctt tttcatttct tttatattgt tataacagtg aataacataa aaattcccga
     5941 caatcattgt aatatttttt tgtcacctgt attagtattt aatttatagg aaactataaa
     6001 aactccctag tgaatttaca cctttgggag ttcctgtttg gacggtatgc ataatacatt
     6061 taaatcaatt taacattaac acctcagcat atggtatctc cacatatata cggtctttct
     6121 ttggatagta ctcaatattt atgttgatag cacttccatt gtctaatata gctttagccc
     6181 ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc atcacatgat cgatttaagt
     6241 tgtactgttc ttgtaaaatc tgtgttacca atggttttga tgacttctct aatccagatg
     6301 tacctgtcat ttttgaaaaa ataataccta taatagccaa gacaacaata cctataatga
     6361 caaacttctt gttcatattt actttattca actttttatc tcctatctcc aaaataattt
     6421 gttcttatcc ctcttatcag tacattgaca tccctaaaag tacaccaaag attgctccag
     6481 ctacccctgc tatcatcaaa atcgtaccca ttttctcatc tttttctcga tataagtagc
     6541 ccataataac agcaacagca ccaaatacaa caggaataaa tgctaacgat actaccatag
     6601 aaatccaacc aataatcaaa tacagcttcc ctgtctcttg tccatgtacg atttgttcct
     6661 tcatttcatt aattgtctgc tcagtacttt tctttactgt gtctgttccg gttgtctcat
     6721 ctgtttcttg tagtgctcca caaaactggc aatacttact ttcatctgtt atttctttgc
     6781 cacattttct acaatacatg tcttctcctc ctaaaatcat tcaattgctt attagttaac
     6841 tgtttacttt attaaataca tcagaaaaca gctcattcat tggtacactt aatatctcag
     6901 aaagcatctt catttgatag ggtttaggaa tcgttctccc tgtttgccac gaacttacac
     6961 attgctgtgt aacattcatt ttttttgcta actccgtttg agaaagtcct ttttcttttc
     7021 tgtatcggct gagattattt acttttttca tgattctccc ctcctgtttt attatataca
     7081 acaatatgat gtatttcaac tcataaaata taaaaaactt caaataccat tacaaataca
     7141 acaacttgtt gtactatact gagatggggg tgtattaatg aaacagaaac aatatgcaaa
     7201 ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa aaagaattag ctgaaaaaat
     7261 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagagc ctaacctcga
     7321 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     7381 tgtcgtagat tatgaggaag agtttttggc tctctatcgt tctcttagca ctgaagatgc
     7441 agttcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     7501 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     7561 ataagattaa gagctatgga taacctgtct agtttaatac aattgggtct tttctcttcc
     7621 ttactctccg tccatcatct cttgttacac ttgtaaattg atacagagga actatcccag
     7681 ctcctgataa tatcaattct tttattgttt catctatccc tgcccctttc gctaaatata
     7741 ttaaaaaact actacttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     7801 caagagtgaa tccttgttta acaaagtagc ctctgttttt tattaaagca tttttcacgc
     7861 tgttatcccc aacattacgt aggcgttcac aaacagtgtt aataagttct ttctctgtca
     7921 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     7981 attgtttttc cttgctgagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     8041 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaattt
     8101 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     8161 cgtctaatcg atagacattg cttctcttat gattaaagtt tcctaacgct ttctcttcct
     8221 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     8281 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     8341 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     8401 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     8461 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     8521 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     8581 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     8641 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     8701 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     8761 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     8821 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     8881 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     8941 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     9001 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     9061 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     9121 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     9181 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     9241 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     9301 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     9361 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     9421 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     9481 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     9541 tttcttgatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtttcctcct
     9601 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     9661 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     9721 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     9781 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     9841 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     9901 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
     9961 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
    10021 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
    10081 catcgacata cacataatac ctcgataaaa aaggggtgtc aacagctttg taaaatttat
    10141 ttacaacacc ccttttttga ggtatactaa ttttaggagg tgtggtcaat atgataaaac
    10201 gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata aaagctataa
    10261 gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac ggtcaattaa
    10321 ttgacaaaaa aactatctac gaatgggaga agggaaccta tttaccaata ccagagcgat
    10381 tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt ggcaatgtag
    10441 aagaatatat tttgggaatt attctttata gagatagcat tgtactagat ggtataacat
    10501 ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta cactctaatt
    10561 tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa tttattgcta
    10621 acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag
    10681 aagaattatt catcaatgct attgtagaag aatttgacaa taacattctt ttcctcacta
    10741 gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt caattgaaag
    10801 acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac aagctagaac
    10861 aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca
    10921 tctagtggta agtactcctt gtaaactaag aggagatgat ttaatggcaa aaatatcaaa
    10981 gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg ccaccgatcc
    11041 acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac aagaagctca
    11101 aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga aagcacctga
    11161 tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca gtgttaaagc
    11221 ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag catttggtaa
    11281 gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag
    11341 taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac gctacggggt
    11401 tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga
    11461 gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac tggatgcttt
    11521 taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat acacaggtat
    11581 gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta ataaagaact
    11641 acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc aatctccgaa
    11701 aacaagagct tctagacgag ttatctcttt agatactgag actctttcta tcctcaacaa
    11761 ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta gctctaaaga
    11821 gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg ttaatgattg
    11881 gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg ggtttagaca
    11941 tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc aacaacgatt
    12001 agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa ataatgcacc
    12061 taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga gctggtcaaa
    12121 atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat atatcaaggg
    12181 ttctagctcc tataacaggt ttacccaaca ctgccttcca tctcataact gatcaatcga
    12241 ttgagttcga ctgcatattc cattggaagt tctttggtga atggttcaac aaagcccatg
    12301 acgatcattt cagttgcttc agattcggtc aggcctcggc tcatcagata gtagagttgt
    12361 tcttctgaga tttttgagac tttggcttcg tgttctaaag agacttggct gttgtggatc
    12421 tcattgaatg ggatcgtatc ggatttcgac cattcgtcca tgatgatcgt gtcacattcg
    12481 atatgagaga tcgaacccgc actgtttttg gcaaaggtta cttgtcctcg atagtttact
    12541 tctccgccgt cttttgaaat tgatttagag acgatagagc tagatgtatt tggtgcattg
    12601 tggatcattt ttgcacccgt atcttggatt tggtttgctc ctgcaaaggc gatggagagc
    12661 atggtgcctc gtgcaccttt tccatctaag taaacacttg ggtatttcat cgttgttttt
    12721 gcacctaggt ttccgtcgat ccattcaacg gttgcgcctt cgtatgcttt tgctcgtttg
    12781 gttactaagt tgtacacatt gtctgaccag ttttggatcg ttgtgtaacg gcaatacgca
    12841 tcttttcttg taaagatttc tacgatggcg gcatgcaggc tgttacttga ataagtaggt
    12901 gccgtacaac cttcgacata gtggatgctt gctccttcgt caacaatgat cagcgtacgt
    12961 tcgaattgcc ccgtgttttc cgcattgatc cggaagtacg tttgtaatgg aacatcgact
    13021 ttgacgcctt ttggcacgta aataaaggtc ccacctgacc atacggctga attcagcgct
    13081 gctaatttgt tgtcggttgg cggtacaagt tttgaaaagt actctttgaa taattcaggg
    13141 tattctttca aagcagagtc agtgtcagta aagacgattc ctaacttttc gaattcatct
    13201 ttcatattat ggtacacgac ttctgattca tattgtgcgg aagcaccggc tagataagct
    13261 cgttcagctt ctggtatacc aatcttttca aatgtatctt tgattttttc tggtacgtct
    13321 tcccagtcac gagcgggttt gtcacttggt ttttggtaat atttgatggc atcgaaatcg
    13381 atgtctgaaa ggtcggggcc ccatgtttgc attggcattt tgttaaatgt ttctagagat
    13441 ttcagacgga attcaagcat ccattctggt tcgcctttga ttcttgacat ctcacggatc
    13501 acttcttctg tcaagccttc tcctgtactg aaaaccggtt cgacatcatc gtgaaacccg
    13561 aatttatatt cttctaattc tggtacgccc atgttctcac tcctcttcct tgtggtaatg
    13621 atggaaagct gcggtaccat cgttttccat tgcttttcct agtgctttcc aggcaagtgt
    13681 cgcacatttg atccgagcgg gaaatttagc tacaccacca agcatagcag catcgcctaa
    13741 ttggtcggga tctttgactt cttttccttg gacaagctgt agaaagtcta cagttaaatg
    13801 ttctgcttct tctattgatt ttccgatgac ggcgtctgtc atcatcgagg cacttgccgt
    13861 actgatggag cagccactgc cgtcaaaggc gatatttttg atgattccgt cttttacatc
    13921 gacttgcaat tcgatcacat ctccacatgt ggggttgttc aattcgatct tgcttgtcga
    13981 gtcagtcaag cttccacgat gatgcggatg ggaagaatga tccaagatca cttgacggta
    14041 taaattgtct aatttagata gtcccatgtt ggaaaaactc ctttgttgcc atgattgctt
    14101 caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata aaagctcgcg cgagcagtcg
    14161 ctgatacttc aaggtacttc aatagcggtt gcgcacaatg atgacctgct ctgacggcta
    14221 ccccttccat gtctaaagct gtcgcgacat catggggatg taacccatcc aaattgaatg
    14281 caacgactcc tgtccgaaaa ttcgggtctt gtggaccata aacagtcaat ccatcaattg
    14341 cttgtaattt tggcaacaca taagcggcta gttcttcttc atatcgatgg atattgtcca
    14401 taccgagttt ttgcaaataa tcgattgcat ggcctaatgc gatagcgccg gcgatatttg
    14461 gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt actgtcgtat aattcgacaa
    14521 aatcgatcat ttcgccacca aattcaacag gttccatttc ttctaaccat tttcgtttac
    14581 catataaaac accaatacct gttggtccgc acattttgtg tccgctgaaa gcaaagaaat
    14641 cacagtctag tgcttggaca tcaatggcca tgtgcggggc agattgagca ccatctacta
    14701 cgatgacccc gccgttttca tggacaagtt cagctaattc ttgaacagga ttgaagacac
    14761 ctaagacatt ggatacatga gcaatggaga cgattttggt tttgtccgta atctttttcg
    14821 ctgcatcctc catatccagg aagccttctt ctgttagttc gatatatttc aagacagcac
    14881 ctgttcgttt tgccaactgc tgccacggga taacattaga atgatgttcc atgtaagaga
    14941 gcactatctc gtctcctgct ttgatatagc ggtcgccgaa ggttctggct acccaattca
    15001 agcttgtcgt cgtaccacga gtaaacagca cttctgctgt ttcttttgca tggatgaacg
    15061 cccgtacttt ttctcttgct tgttcataat catgggtcgc gcgttctgct aacgtatgga
    15121 caccgcgatg aacattggcg ttatcgtgtt cgtaatagta cgctaatgcc tggagtactt
    15181 gttttggctt ttgggtcgtc gctgcattat ccagataaac taaaggttca tcattgacgt
    15241 cttgaaataa gataggaaaa tcattgcgta gctttttagc atcgatcatc cattcaactt
    15301 cccttcgatt acttcgacta attctttccg aacttgttct acagggattg ctgttaagac
    15361 agaaccgagg aatcctcgaa tcactaagcg ctctgcttct tctttatgca atcctctact
    15421 catcagatag tacatttctt ctggatcaac tcgtcctaca ctggcagcat gtccagcagt
    15481 tacttcgttt tcatcgatca aaagaatagg attcgcatca ccacgtgctt tatcagaaag
    15541 catcaagaca cgactttctt gttgggcatc tgcacctttt gcccctttca agatgtggcc
    15601 gataccatta aaggtcaatg ttcctttttc tcggatcact ccgtgttgca aaatatgccc
    15661 gatcgtatga ggggctttat tggttactcg tgtatcgatt ccttggattt gtctgcccga
    15721 gctgattgcc acgattttga cttctgcgtg tgcaccttct cctgctaaat ctgagtcaaa
    15781 gtcagcgatc acatgaccgt cattcatgac accaattgcc cagtcgacag aagcatctcg
    15841 taaaatgtgc ccacggcgat tcatataaga agtaatattt tctcctaatt gatctacggc
    15901 tgaatatttg atttttgaac cagctttagc gatgacttcc acgatgatat tacctgatga
    15961 ttttgctact tgttctttgg ttgtctggaa acgttccaga taagaaaatt cactatgttc
    16021 atctgccacg atcaacacat gcttgaagaa gtgctcatcg gatgcgccat cttgaatgaa
    16081 taaggactcg agtggttcat cgatcacgac atttttaggc acatacagga aaataccgct
    16141 gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg ttcatgttta ctgctttttt
    16201 catataatat tcttggacta gttctggata ttcgatcatt gcagtgaaca agtcggtaaa
    16261 aatgactcct ttttctgcta attccacagg cagttgttca aaaatcgtcc atgaaccttg
    16321 ttggacaagc acaggattgt ctttcattgc atcaaatgcc ggaatcgtgc ctgtttcact
    16381 gatggtttgt gtttctttca catcaaataa cgaccagcga tggaatttca cccgatcgat
    16441 atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct tgacggaagg ttctcatcca
    16501 atcaggttcg cctttactta aagaaaaatc atttaccgcg tcaagataat cgagccagtt
    16561 tttttctttc attcttgctc ccccttacgc ttcttcttct ttatagtcga ttcctaattc
    16621 tttactgata ccagcgtagc cttctgcttc cagacgttta gctaggtcag cattccctgt
    16681 catgactact cggccttcca tcatgatgtg aacgacatca ggtgtgatat agttcaataa
    16741 tcgttgatag tgggtgatga tcaatgcacc gaaattgtct ccgcgcattt catttactcc
    16801 tttagcaacg actttcaaag catcgatatc cagacctgaa tcgatttcgt ctaaaattgc
    16861 aaatgtaggt tctaacatca acaattgcag gatctcattc cgtttctttt ctccgccaga
    16921 aaaaccttcg ttcagataac gttcagccat ttcttctggc atatttagta aagccatttt
    16981 ttcatctaac tttttcaaaa atgccataac agaaattttg tcgttttcat cacgtttcgc
    17041 attgatcgct gcacgcatga attccgcatt cgtgatacct ggtatctcac ttggatattg
    17101 catagctaaa aataagccaa gtcgcgcacg ttcatctact tccaagtcta aaacattttt
    17161 cccgtcaaac aaaatttcgc cttctgttac ttcatagttt ggattgccca tgatagcggc
    17221 agataaggta gattttcctg tgccgttagg tcccatgatg gcatggattt ctccggtttt
    17281 cattgttagg tttacgcctt taaggatttc tttttcctcg atcgacacat gtagattttt
    17341 tatttctaag acagacatgc attatacccc caaattctct ttcgttctca ctcattttag
    17401 actaattgag aatgaagcgc aatg
//
LOCUS       DAXWLH010000052        17023 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXWLH010000052 DAXWLH010000000
VERSION     DAXWLH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.052"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..418
                     /locus_tag="ACGE36_001286"
     CDS             <1..418
                     /locus_tag="ACGE36_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383690.1"
                     /translation="LLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTLI
                     IAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPK
                     IFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            513..1397
                     /locus_tag="ACGE36_001287"
     CDS             513..1397
                     /locus_tag="ACGE36_001287"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8383691.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1621..4788
                     /locus_tag="ACGE36_001288"
     CDS             1621..4788
                     /locus_tag="ACGE36_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFF8383692.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4856..5353
                     /locus_tag="ACGE36_001289"
     CDS             4856..5353
                     /locus_tag="ACGE36_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFF8383693.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5372..5836
                     /locus_tag="ACGE36_001290"
                     /pseudo
     CDS             5372..5836
                     /locus_tag="ACGE36_001290"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5833..6591
                     /locus_tag="ACGE36_001291"
     CDS             5833..6591
                     /locus_tag="ACGE36_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8383694.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6677..7417)
                     /locus_tag="ACGE36_001292"
     CDS             complement(6677..7417)
                     /locus_tag="ACGE36_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8383695.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7478..8731
                     /locus_tag="ACGE36_001293"
     CDS             7478..8731
                     /locus_tag="ACGE36_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8383696.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9053..9280
                     /locus_tag="ACGE36_001294"
     CDS             9053..9280
                     /locus_tag="ACGE36_001294"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8383697.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9403..9729
                     /locus_tag="ACGE36_001295"
     CDS             9403..9729
                     /locus_tag="ACGE36_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8383698.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9797..10510
                     /locus_tag="ACGE36_001296"
     CDS             9797..10510
                     /locus_tag="ACGE36_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8383699.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10820..11671
                     /gene="licT"
                     /locus_tag="ACGE36_001297"
     CDS             10820..11671
                     /gene="licT"
                     /locus_tag="ACGE36_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8383700.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11792..13657
                     /locus_tag="ACGE36_001298"
     CDS             11792..13657
                     /locus_tag="ACGE36_001298"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8383701.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13671..15114
                     /locus_tag="ACGE36_001299"
                     /pseudo
     CDS             13671..15114
                     /locus_tag="ACGE36_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15222..15923
                     /locus_tag="ACGE36_001300"
     CDS             15222..15923
                     /locus_tag="ACGE36_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8383702.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16049..16561
                     /locus_tag="ACGE36_001301"
                     /pseudo
     CDS             16049..16561
                     /locus_tag="ACGE36_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5668 a         2873 c         3678 g         4804 t
ORIGIN      
        1 tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc
       61 aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg ttttggtgtt
      121 aggcacacta attatagcgg ggattgtcgc atttattatt caaggccgtg acatattaac
      181 agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga
      241 taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc
      301 acctaagatt tttactttat taggtataca atgcctatca ttttctttta tttcctatat
      361 cattgtttta attttcctca gtttcatagt atggatgttt tatcttgtca aaaaataaat
      421 gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata
      481 ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc
      541 ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat
      601 cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta
      661 ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag
      721 tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc
      781 cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag
      841 cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg
      901 actattttat gaatgtcgtc gcaattggct ctatccctga atcaatcaat gatgtagatg
      961 cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag
     1021 cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga
     1081 gcagtacctt gctgatagga ctaactaact cagtcggcgg ttttgaaaca ttacttccaa
     1141 atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg
     1201 atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag
     1261 cctaccttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga
     1321 atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtattg ccctcacatt
     1381 tacaggttta ttgttaaaat ggaggttgtg accgaggagt tggcgggaga acaccgcttg
     1441 ttcgggataa gggactgtga caagactttt gtcacagcct tttttttgta caaaaaagaa
     1501 atttacacct aatagaaaaa tttttccata aaaatacata ttttaaatat gaaagtacaa
     1561 ttggaaatag attaaaacga taaaataaaa ttgtcaaaac gtcagagagg atggtggaga
     1621 atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg
     1681 gtgacatccg tttttgccga tgtagagaaa acaaacgaat caacgcaatc agagaatcaa
     1741 agttttgaca agactacttt ttcagaagaa tcgattactt ctgaaactac agaatctcaa
     1801 agtgaggctt ctactaataa aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     1861 gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa gagctgtttc agcggatggg
     1921 tacagcgaag ctgccacaac agaagaactg gcgcagcttc tcgcagacga atcagtaact
     1981 aagatccgac tgattcagcc tttgacttta gacagggaat tagagatcaa acgagatatc
     2041 gttattgatt ttggtggatt cgctcacaat ttcggcacac accatattta tatcaatgag
     2101 atacctcagt cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc aggtggattg
     2161 acaccaaata tcgatggaaa tgccattatc attgcttata tgaccgattt ttggggaagc
     2221 agatatcatt ttacgggaga aataaaattt accgggacgc ttgatttgta tgatgggtcc
     2281 aaattaggac tgatttacgc tcctcgtgca actgtcacat tagatggtgt ttccggtgtg
     2341 ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc ctatttttgt
     2401 agaagctatc aattgaatgt gatcaatggt agtcaacttt atggaccgta tctagggaaa
     2461 ttctacggtt ttctagattc aggagatgag actggtagtg gaaaaacagc gccaggaatc
     2521 catatcttaa gcggttcaaa ggtctctctt gattatgatc gggcagatgg cgttagcgat
     2581 gaaggagagg cagtcgatac gctacctggt aatgtcatgt ttgaagtcag tggttcggga
     2641 agcgaatttt cggtaaatac aaagatcaat attaccaatg acaacaatcg cggaatcatc
     2701 caaatgagag gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat caccatcaat
     2761 acagcaacta cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt
     2821 ggtggacaaa tctcggtcaa aatggctgga gatgacgatc aacccgggaa caatgggatg
     2881 cgttttgtag gaaaaggcct ttctttgatc gtagaaggag ccggatcagt tatcgacatc
     2941 gataagcaga gtgggcgttc cagcgcagtt cgttttgaga atggtacgca aaagctgaca
     3001 gtaaccaatg gaggctcatt gagggtaaga aatacaggtg atggcaaatc ttacgtgaac
     3061 agactcaatc aaggaaatca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3121 ccaggttcag ctgattttac agtcactgga gaaaaatcaa atatcgatat acgcgcagat
     3181 tccggagcaa ctgtagatac aactggagat attgatttaa attttacggc aggtgaaaaa
     3241 acttatttag tcatggtcgg gaaaacaggg gaagataaga ccggcatcat cagtggggat
     3301 gtgttgaatg tggacttaga atctcctacc tattttgatt tccaaaacaa acggattgga
     3361 agcgagacca atggcatagg cggctgggta ttccaaggaa atggagattc ttctttaaag
     3421 atcgcccatt cagaaatcgc tttatggcga aaaacaggcg caacattcga taactttgaa
     3481 aaagacccgg attatttttc tgatatgaca gacttatcga tcaatgggac cgacttaggc
     3541 aatttcgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3601 atgaaaaatt ataatcgaat cagtgccaat aatcagctcc cgatgattga atccttaaga
     3661 gtgccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3721 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttacgca
     3781 gatacgacga aatcatctgt agaaattact gctctcacgc aaggacaaac agatgaaaat
     3841 gaaggggcag gatataaccc atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa
     3901 gtacccaatg atgagagatt aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa
     3961 gtaagaggtg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4021 cctcctgaac ctgctaattt aagtaccaca ttgatcaatg cagcttctcg cacgatcaca
     4081 ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa
     4141 acaactattg atgatacagg ggaatttgtt ctttctatcc cagaaaatca actttttgca
     4201 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctactgc taagggtgtg
     4261 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     4321 gtttatcatg atgccccgcc ttttgcacct gctacgaaga tcgtagtaga aggtggtctc
     4381 tcatttcttg ccccttccaa actcgatttt ggtcagatca aagcaactgg tttgacacaa
     4441 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaacg
     4501 aaaaatccgt ggatcttaac gataaaatta tctcagcctt tcacaaatga tgcaggattt
     4561 tctatgcagg atgtcctata tttcaagaaa ggagaggagt atcaattagt gaatgaagaa
     4621 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aagcagatta ttcagaatat
     4681 ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     4741 tactcagcag agataacatg gacattagca gatgcgccga cagaataaag atcaaggagg
     4801 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttatgat
     4861 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     4921 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     4981 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     5041 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     5101 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     5161 aacagtggga gaaataactg ttcctcctca aagtgcggtc actgagacac tttcactgaa
     5221 agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa
     5281 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     5341 aatcttgatt tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa aaaaagctta
     5401 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     5461 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     5521 cctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     5581 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     5641 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     5701 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     5761 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     5821 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     5881 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     5941 tctgtgacca aaccagatgc tgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     6001 ccaaatgatc ctgatactcc ttttacacct gacgaaccaa atcagggaac atccggatta
     6061 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     6121 acagtatatg ctgcattaca aagcggaacg gacagtactg gagcacaaac acaagtacag
     6181 aatttcgtac aagtaacgga taaaagcgga aattatgccg gttggaaatt atcagtcaaa
     6241 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     6301 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaatgcc atctaccgtt
     6361 tctggaactg gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga tctagtaact
     6421 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     6481 gggagacaat cagtggaatt atttgttcct gtatccaatt atgctgtaga caactacgtt
     6541 tcaacattca catggtcgtt aacagatgct cctacagaag aggaagcgta aaatcatatc
     6601 aacgatttca gggagtggag ccgattcaag gcccactccc tgaaatctgc tatgcttacg
     6661 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     6721 tcttttcaaa gatctttcga ccaacgagta aggagtcacc gttgaaaaat tctaacacat
     6781 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaaaagta
     6841 aaaagtaagc atacttttct aactctgtct tcaattgttt taaagaagta ttcactaatt
     6901 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     6961 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     7021 cgacatttac gatctgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     7081 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     7141 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     7201 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     7261 aaacatcgtg attatcaata gtgcccaact ggatcaactg attgatttga gagatagaag
     7321 tgagctgcac gatattctcg atatgatgat tgaccaattc tctgatgatc cattcgctgt
     7381 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     7441 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     7501 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     7561 agtaaaaaac agctttggtt gtatagcttt gatggtggtg acacttttac ttcctatccc
     7621 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     7681 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     7741 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     7801 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     7861 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     7921 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     7981 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     8041 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     8101 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     8161 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     8221 aatattagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     8281 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     8341 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     8401 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     8461 tcagagtccg attacgatca atctgctgga agaaaaagag ggatcacgtt ttagtatagc
     8521 gaacaaggca aatccaacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     8581 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     8641 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     8701 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag tttgaggaaa
     8761 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     8821 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     8881 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     8941 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     9001 tcgcttattt taggcgtgga gaagcaagca aatccctacg atcacaaaaa aaatgctgac
     9061 ccagcagtta agagagttag aacaagatga aagtatccat cgaaaagcct ataaccaagt
     9121 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     9181 agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga
     9241 cacgctactg catacacatg gtggctacct aaattattga ttttctatga catttatttt
     9301 aaataaaaga aaacaggagt ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg
     9361 tatgattaat caagtaagaa gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa
     9421 gtcaaccagt ttagagatga acgtaattgg cgacaatttc ataacgaaaa agatttggct
     9481 atctctattt ctttggaagc atctgaattg ttagaacttt ttcaatggaa aacgccggaa
     9541 gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt attgatctat
     9601 tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga agaaaaattg
     9661 atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca
     9721 gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag
     9781 tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct
     9841 cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga tcaaggatta
     9901 ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag aagatctgaa
     9961 aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa tgacgacaga
    10021 aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta gtatcgcagt
    10081 cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt cactacttta
    10141 cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg ccttagcaag
    10201 ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag agatgggtgc
    10261 caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa
    10321 agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact
    10381 aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag aactggaaag
    10441 tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc tagagacaaa
    10501 aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa aggatatgaa
    10561 tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg catctctgat
    10621 ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat gaaaaggctc
    10681 gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg ttactgtacg
    10741 gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt ttttattttt
    10801 cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg tcgtcatttc
    10861 gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa
    10921 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgttttaa gagattcttt
    10981 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
    11041 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
    11101 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
    11161 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
    11221 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
    11281 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
    11341 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
    11401 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
    11461 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
    11521 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
    11581 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
    11641 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
    11701 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
    11761 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
    11821 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    11881 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    11941 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    12001 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    12061 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    12121 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    12181 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgcag cagatggtgt
    12241 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    12301 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    12361 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    12421 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    12481 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    12541 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    12601 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    12661 aagtttatgg caagtattcg tgatgttcgg catgcattgg ggattcgtac cgatcatgtt
    12721 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    12781 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    12841 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    12901 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    12961 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    13021 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    13081 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    13141 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggagaac cgattacttc
    13201 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    13261 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    13321 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    13381 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    13441 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    13501 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    13561 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    13621 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    13681 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    13741 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    13801 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    13861 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    13921 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    13981 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    14041 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    14101 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    14161 cagaaagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    14221 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    14281 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    14341 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    14401 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    14461 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    14521 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    14581 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    14641 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    14701 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    14761 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    14821 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    14881 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    14941 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    15001 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    15061 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    15121 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    15181 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    15241 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    15301 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    15361 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    15421 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    15481 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    15541 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    15601 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    15661 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    15721 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    15781 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    15841 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    15901 accatattga ttatgaaaca taaataaaaa aagaggttgt gaaaaagctg ccctgcatca
    15961 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    16021 accgcagata cacggctgtt tctggattac catttattct ggataagggg gtgtggcacg
    16081 attttcagaa ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc
    16141 attcggcttg acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tactcaatcg
    16201 tcaagaaatc gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg
    16261 atgtttccct atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg
    16321 gacagtaggt ggcggattaa gcatgttttg gggcgcagat agccgtattc cggcatcggg
    16381 aatgtttgca gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg
    16441 ttcttttgtc acaggaataa ccattttatt attgaaaaaa ccagtagatc cgaatgcaga
    16501 aatcatccaa gaagaaaaag aggaagaaga catatcttgg gatgatttga caattagtta
    16561 ggagaggcag aatggattac aagaaaaaag tacaatagat gtaccttgag acaggaatgg
    16621 tatgaaagaa tcagagttag aaagaatcga atatacagat tttggtttag gagcgatcga
    16681 aactgtccag agcatcggca ccctccttga aatgacgatg aaggaaaaat gagaaccttt
    16741 cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct
    16801 gtactattcc aaaaaggaaa gaaagttatt atacagtaaa aaaagaaatt attcttcatg
    16861 ctagagatca gtacacgttc gagcccaata tcaaacacta gttccaggca ggcgcaacag
    16921 gtgcggttat cactgaattt tcttccagca gtgacgattc aagtgatatt tttacaaatc
    16981 cagctattta tcgttaacaa aaagattttt aggagaagct gat
//
LOCUS       DAXWLH010000053        16632 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXWLH010000053 DAXWLH010000000
VERSION     DAXWLH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.053"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(46..780)
                     /locus_tag="ACGE36_001302"
     CDS             complement(46..780)
                     /locus_tag="ACGE36_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8383703.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="ACGE36_001303"
     CDS             1106..1516
                     /locus_tag="ACGE36_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFF8383704.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="ACGE36_001304"
     CDS             1509..2210
                     /locus_tag="ACGE36_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFF8383705.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3823
                     /locus_tag="ACGE36_001305"
     CDS             2210..3823
                     /locus_tag="ACGE36_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383706.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3813..4034
                     /locus_tag="ACGE36_001306"
     CDS             3813..4034
                     /locus_tag="ACGE36_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFF8383707.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4031..4792
                     /locus_tag="ACGE36_001307"
     CDS             4031..4792
                     /locus_tag="ACGE36_001307"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFF8383708.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5173..5682
                     /locus_tag="ACGE36_001308"
     CDS             5173..5682
                     /locus_tag="ACGE36_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8383709.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5752..6291)
                     /locus_tag="ACGE36_001309"
     CDS             complement(5752..6291)
                     /locus_tag="ACGE36_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8383710.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE36_001310"
     CDS             complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE36_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFF8383711.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7304..8155
                     /locus_tag="ACGE36_001311"
     CDS             7304..8155
                     /locus_tag="ACGE36_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8383712.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8183..8818
                     /locus_tag="ACGE36_001312"
     CDS             8183..8818
                     /locus_tag="ACGE36_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFF8383713.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8838..9611
                     /locus_tag="ACGE36_001313"
     CDS             8838..9611
                     /locus_tag="ACGE36_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8383714.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10019..10816
                     /locus_tag="ACGE36_001314"
     CDS             10019..10816
                     /locus_tag="ACGE36_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFF8383715.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10794..11207
                     /locus_tag="ACGE36_001315"
     CDS             10794..11207
                     /locus_tag="ACGE36_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8383716.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE36_001316"
     CDS             11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE36_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8383717.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE36_001317"
     CDS             13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE36_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFF8383718.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15984..16544
                     /locus_tag="ACGE36_001318"
     CDS             15984..16544
                     /locus_tag="ACGE36_001318"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFF8383719.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5399 a         2970 c         3602 g         4661 t
ORIGIN      
        1 cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag ccataaataa
       61 taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa ctggtaccca
      121 tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt gtacaaaacg
      181 tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa gtgaagcaac
      241 taatgtccat acaaggaaac cgagtgctaa gtgagctgta ccaagattat cttggaagaa
      301 tggtgctttt gtaataccta aggcaccaaa gaataaaacg aatgaaccaa agaattcatt
      361 gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac ctaacacatg
      421 gttcacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga tcaattgtcc
      481 aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc aagggaatag
      541 accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc tgattccacc
      601 aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca gcatccagtc
      661 actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt tacctagaac
      721 gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg agtaatccat
      781 aaatacctcc aaaagttata aaaatgcaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaacca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagctatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agagggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc aggaagggta tctgtaaacg aaatggagga
     1501 aaatcaaatt gactgagttt tttgtgaatc cactatttgg cttaagtttg actttaggag
     1561 tgtatgtatt atctgataaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat cggtacagca aaagcaatcg
     2101 agattggcga aatagaagga atcatttccg gacttgcgat atgcgtaaat gggatattga
     2161 ccgttttgtt gctgccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatacatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaagtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg ggtcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgaccgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaaaaaac
     3781 tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt ttaccagaat
     3841 tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt tatgttgttc
     3901 tcgtatccga tgatgaattt gatggctgtc cagtgacacc ttacttattg atcaaaacaa
     3961 gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca gatgattggg
     4021 cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat ttatcacagg
     4081 agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag atgcttttgt
     4141 atttggatta gatcaaaagt tcggtaaaat ggaagaactc tctttgcgat ttcctgaaac
     4201 ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag cggtatcgaa
     4261 atgccaggat cgttttggtg aagttacgat tttactgaat acatcgggga tattggatgc
     4321 ctacaaacca ttgatggaaa ctgacgaatc gctgtgggat cttatttatg agacgaatgt
     4381 gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc gaaaaagcgg
     4441 aacaatcatc aacatggctt cgattgcggg tttggttgct ggcggaggtg ggatcgctta
     4501 tacaagtgcg aaacatgcaa tcgtcggatt taccaaacaa ctcgctctgg attacgcagg
     4561 acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa tgaatgcggc
     4621 tgattttgaa gggaaaggtg agatggctga gtgggtcgca aatgaaacac cagtcaagcg
     4681 atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc ctcaagcaag
     4741 ctacatacaa ggaacgatca tcccaatcga tggcggatgg ttgttaaaat aaccttgtgt
     4801 ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat gataaaagaa
     4861 agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat tctttttaat
     4921 aatcgttaat attcgaaact aagcggcttt tcacgacctt ttgatataaa cggtgttcaa
     4981 aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc tatttttatt
     5041 tgactattct tattacttgt tgcagggcag ctttttcact accttctgcg gcacttcagt
     5101 tggaattttc ttcccatcct ttggtcgcag tcattaccaa gataaaatta tcttgcaaag
     5161 gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac aacgacagat
     5221 gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt tttgtctgtc
     5281 tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct gtccttgtat
     5341 aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat ggcttctcct
     5401 cttggactga tagatgttgt agtaggaagc gtcagcacgc tgcttgtatt gcttatcaac
     5461 tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggctgtgac agcatgcgta
     5521 tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca actacctttt
     5581 ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt cggtgggata
     5641 attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt ttaatttaaa
     5701 atatggcagc gtggaagaga ctttgagtct tcttccatgc tgcttttttg attaatttgt
     5761 atgaagaaat cgatttagcc gttttttcat caagataccg atataagctg ctccagttgc
     5821 cttgattgct tccggcagta aaaacggcag tagtccactg gcaaatgcag ttgtaaaagt
     5881 aagatcacca cttattttca gccagatcgt tccgcagatc aatgcagcaa gagagcccat
     5941 tagattggca aagattgcat ataagtaatg agcatccgtt ctttcgatca tccaaccagt
     6001 tatccacgtt tgtaagagaa atccaatcaa atatccgcca gttggcccaa ataaaacagc
     6061 tatacctcca gtcatccctg aaaatacagg aagaccaatc aagccaagta aaagataaac
     6121 tacaacagat atcaatccta gttttcttcc gagaaaagtt gctgttaatc caatagcaaa
     6181 tgtttgcccc gtcaaaggaa ccaagccaaa aggaatggtc atctgagaaa ggacagaaat
     6241 aatcgcggca aattccgcag caagtattaa ttctttcaat gttcggttca taatgccctc
     6301 ttttcgttaa cttaatattt ttatttggtt aacgaaattg taaaacaaag tgattacttt
     6361 gtaaatagaa aatataaaaa aagaccagct atccaataga tagcggtctt tttcaagaaa
     6421 gcttttgaaa ttataagctt tggttgtagt attcaacgac aagagcttcg tcgatttctg
     6481 gatatagttc gtcacgttct ggtaaacgag tcaatgaacc ttccaatttt tcagcgtcaa
     6541 agcttacgaa tgctgggcgt ccaacagttg cttctacagc ttcttttact gttacgatgt
     6601 tttgtgattt ttcacgaact gagatcactt gacccacttc tacgtggtat gaagggatat
     6661 caacgcgttt gccatcaact aaaatgtgac catggttaac taattgacgt gcttgacgac
     6721 gagtcgttgc aagtcctaaa cggtaaacaa cattgtctaa acgttgttct agtaagatca
     6781 tgaagttaac accatgttta ccttctttga ttttgctagc ttttacgaac aagttacgga
     6841 attgacgttc gttcattccg tacatgtgac gaagtttttg tttttcagtt aattgcatgc
     6901 cgtattctga ttgtttgcca cggctgttag gtccatgttg acctggtgcg taagggcgac
     6961 gtgctaattc ttttcctgtg cctgacaatg agatgcctaa gcgacgagaa attttccatg
     7021 atggtcctgt ataacgagac attaaaattt cctccaatga atataagttt ttggagtaaa
     7081 ataatccgtt gaaaaattca taatcatata gtttgttctt caatcttcac ctttgcagcc
     7141 gcggttacac aatcgaaccc gagacggggc aacaaactag ggatgagtta ttatttttct
     7201 gctgcattat tttacacaaa ctacagtata tgatagatca tccacgaatg tcaagataat
     7261 tagtacataa aaacgcttta caaacctagg ctgtctttat aaaatgagag ggcatgtgca
     7321 aatggaaaaa agcgaaggaa gtttaaggag aaaatcaatg aatccggtat caccattatt
     7381 tgagaaaatc gatgcttcaa ttgaaaagaa ggcgaatctt tttgaaaaca gcttttcacg
     7441 ttacgctgtt cgagcggttc tcgcctgttt gtttttgact ttaggaacgg ctgtggcatt
     7501 tgcaatcgcg atgaaggggg aagatatcgc gcatggtcta ggaaagattt tatacgcatt
     7561 tatgttcagc tggggtctgg tcatgattct atacatgaac gctgaattag gaacatcgaa
     7621 tatgctttat atgaccgtgg gtgtttttcg gcagagactt gctttaagta aggcagccaa
     7681 gattctattt gcctgtattt ttttcaatat gattggcggt atattatgtg gatttcttat
     7741 ttctatgact acgccatttc aagatttaac gttggacaac tttatgttag aatcaatagc
     7801 agtaaaacta gcaaaacctg cagcaacgat tttggtggaa gggatgtttg cgaatatcgt
     7861 tgtcaatacc gctgtactga tcagcatgcg gatgaaagag gatgccggga aagtgatcac
     7921 agtcgtattc atcattttca tttttgcttt tttaggatat gaacacgtga tagcgaattt
     7981 tcctgccttt acgcttgctt actttgcttc tcaaggacag acggatggaa tgacagtcag
     8041 caatgtgtta cataatttgt tttttgcttt ggcaggaaat tatattggtg gcggtttagt
     8101 aatgggactg ggatatgctt ggctgaatca gtcaaaatca tcctatgtgg actaaaaagt
     8161 cgttcattaa aggggaagtg ccatggaagt tttagttttt ccaaaaaatg aggaaaaagc
     8221 gttacaaatg gaaaagtaca tgaaaaatct tttttccttt gctggagtac ctaagccgga
     8281 acgcgctctt ttacaacaac cgttactgga tgagagcaaa aatttatcta tttctgaagt
     8341 tttagctttt gtttattatt attatgaaca aatcgaaaga gagtatcagt atgtagcgat
     8401 cgatttagtc cagaaaaaca tcaaacggct tacatttgaa gacttagtcg tattgttgcc
     8461 actagttcag aaaaaagaat ggtgggacag catcgactct tggagaaaag tatacagcac
     8521 atggtgtaaa gcctacccag agcagcgtga agaagtcttt caattgtttg aaaaaaagga
     8581 agatttttgg ctacgtcgaa tagcaatcac gttgcaatta ggttataaac agcaaacaga
     8641 cacaggacta ttgatccgag caatagacga agatcgtcaa acaaaggaat tctttatcca
     8701 aaaagcaatt ggttgggcat tacgcgatta cagtaagacc gatgccgatt gggtaaaaag
     8761 acagctgaca acagatttga gtaagttagc tgttcgagag gcaagtaagt atttataaga
     8821 aagcgagacg acaagagatg agacgcaaat tatttgcttt tgatattgat ggaacgttgt
     8881 tagattcaaa caaacaagct ttagatagca cgagagaagc attggaaaaa ttacgcaaag
     8941 ccggtcatct ggtcacgatt gctacagggc ggagccgatt tcatgcgcaa gagatcattc
     9001 gcgatcttga ttttacgaac tacattttat gtaatggagc ggccggtttt ttagaccatg
     9061 aacaagtata taagaattta ctagatcgcg accagttgga tgcgttcgtt caagaagcgc
     9121 atcatgagca gatcgataca gcttttgtca gcttagacaa tatcaagcgt tcttcttcca
     9181 atgatgtaac ggtgatggaa gaagcgatgc attctttcgg tgctgtcttg cctgaattag
     9241 atcagttttt tgcagaggaa caagaaatat accaagcgtt agcatttttc gatcaaacat
     9301 atgatgggaa gttctcttca tatgataaaa ttcgatttgt ccgctggcat gagaacagtg
     9361 tagatgtcgt acctaaaaat ggttctaaag cagcgacaat tctgcactta gcagaacaag
     9421 taggcatccc tcatgaagac atcatcagct ttggcgatgg acaaaacgac cgtgaaatgt
     9481 tgcgcatgtc aggaattggg atagctatgg ggaatgctgt gccagaagta caagcagaag
     9541 ctaagatggt cactgataca aacgatcagg atggaatttg gaaggcattg aaagagttaa
     9601 gcgtgattta aaagaggttg tgaaaaagct gttctgcatc aagtaataag cccagccaaa
     9661 caaaaatagc tcctcctatt tttcgttttt ctgggcttat taccgcagat gcaagctttt
     9721 gaacaccgtt taaggaagag gtcgtgacag aagtggtcag tttcaagaaa taagaaggga
     9781 tttacgaaaa ttgcttctca aatttttgta aaattacggc ttatttctga agaagtagat
     9841 gaaacaacgt ttattcggga aatgggactg tgacaagatg tttgtcatag tcccattttt
     9901 ttattttatt tttcaaaact aagaaatacc aggaaagagt tccttttgtt tgaccttttc
     9961 tttcttattg aaaacaacta taatatagag tgatgttaaa agagaagagg aatttagtgt
    10021 gcgggtattg tttattggtg atgtagtagg ttcaatggga cgagagatga ttactgaata
    10081 tcttcctaga ttgaagaaga agtaccgtcc gcaagtaacg attgtaaatg gtgagaatgc
    10141 cgcttctgga cgtggaatca cagaaaaaat ctataaaaaa tttttgcaag acggcgtaga
    10201 tgtcgtgacg atggggaatc atacgtggga caaccgcgga atctttgagt ttgtaaatga
    10261 agctaaaaag atggttcgtc cggcaaattt tccggaaggg acgcctggac aagggatggt
    10321 ctttgtcaaa gtgaaccaag tcgaactggc agtgatcaac atgcaggctc gttcttttat
    10381 ggtggacttg gatgatcctt ttcgtaaaat gaatgagctg gtagaagaag ccagaaaacg
    10441 tacgccgatc atttttgtag attttcatgg agaaacaaca agtgaaaaac aagcaatggg
    10501 ctggttttta gatgggaaag tttcagcagt ggtcggtaca catacacacg ttcagacaaa
    10561 tgatgcgcga attttgccaa gaggaacagc gtatctgaca gacgttggga tgacaggtcc
    10621 ctatgatggt attttgggta tgcgcagaga acctgtaatc gaaaagtttc tgaccgcatt
    10681 accaaaacgt tttgaagttg tggaacaagg ccgatcgatt ttaagcggct gtatcttgga
    10741 attggatgat acgacaggtc atgcaaaaaa aatccagctg attcagatca acgatgatcg
    10801 accgttcatg gagtagaaag agaggcagtt ttttgcagca ggagccggaa attcaaaaag
    10861 aagtaaggaa actgacaaaa cttttaagag agaatgaaac gatcattcgc tacaaagaac
    10921 ttgaagaaaa gatccagcaa aaccagtatt tagctgagtt gagagaaaaa atcaaacaag
    10981 cgcaaaaaga tgctgttcat tttgcccact atgacaaacc agcagcagaa caagaagcaa
    11041 tcaaacaagc ggatcaattc atgcaagaat ttgatcagca tccgcttgtt gtagcttata
    11101 gaaaacagct gttggaagca gatgatctgc ttcatcattt aacaactatg atacaagaag
    11161 aaatcaatgg acaaatagag gaggaaaaac atgcctcaaa aaactaaaaa tactcctatg
    11221 atggaacaat atctaagcat caaggcccaa taccaagatg cttttttgtt ctatcgttta
    11281 ggagactttt atgaactatt ttacgaagat gcgatcaaag cgtcacagct tttggaatta
    11341 acgttgacga gcagaaatcg aaatgcagaa gaacctattc ctatgtgcgg tgtacctcac
    11401 catgcagcac aaggatatat cgatgccttg attgaaaaag gctataaagt agcgatttgc
    11461 gagcaggtag aagatccaaa gacgactaaa gggatggtca agcgcgaagt gatccagcta
    11521 gtcactcctg gtacagtcat ggacagcaaa ggattgtcgg caaaagacaa caatttttta
    11581 acagctgtag ttagtcaagg aaatgaattt ggctttgctt atgccgattt aagtacggga
    11641 gaattgaaga cggctcggtt gcatgatgaa gaagctgttt taaatgaagc ctctgcattg
    11701 cagacaaaag aactcgtatt aggcagtgag atcactgata cccttcgtga acagctaagc
    11761 gggcgtttag ggctagtctt ttctcaacaa aatgagatgg aagaaaacgc ggaatttagc
    11821 tttttgacaa gcgaactagt cgatccgctg aaaaaagaag caaccggcaa actattgact
    11881 tatttatcgg tgacacaaaa gcggagttta gctcatatcc aaaaagcagt agaataccaa
    11941 ccagatcatt ttttgaaaat ggattattat tcaaaattca acctagaatt aagccaatct
    12001 attcggactg ggaaaaagca cggcactctt ctatggttgt tagatgaaac aaaaacagca
    12061 atgggtggcc gtttactaaa acaatggcta gaccgtccgc ttatccaggc aaaacaaatt
    12121 aaagcaagac aagaaatggt cgcttctttg ttagacagct attttgaacg tatcgatttg
    12181 cagtcagcat taacgaaagt ctatgatctg gaacgcctag caggtcgagt agctttcggt
    12241 aatgtcaatg ggcgagactt gatccagctg aaaaattctt tagaacaagt gccaatgatc
    12301 agagggttga ttgaaggaat cgatcaaggt caatggcaaa gtctgctttc agaaatgcag
    12361 ccgatggacc atctcgtcca attgatcgac caagctatcc aagatgatcc gccgttacaa
    12421 ataacggaag gaaacatcat caaagatgga ttcaatgagc agctcgatat gtaccgttct
    12481 gctatgagaa acggaaagaa atggttggca gaacttgaag caaaagagcg tcaagaaaca
    12541 ggaatcaaaa acttaaaaat cggatttaac cgcgttttcg gttattatat agaaatcaca
    12601 aaagcaaatc ttggaaatgc agaactggag aagtatgaac gaaaacaaac tttagcaaat
    12661 gcagagcgtt tcatcacacc agaactaaaa gaattggaga cacaaatcct cgaagcagaa
    12721 gaaaaatctg ttgatttaga atatcagttg tttttagctg tgagagaaga agtcaaaaag
    12781 gctatccagc cgctacaagt acttgcaaaa gcaatcagtg cagtagatgt gctacaaagt
    12841 tttgcaacga tcagcgaacg ctatcagtat gttcggccag aacttgtcag tgacaagcat
    12901 cagcttctga tcaaagacgg acgacatcct gtagtagaaa aagtattagg acatcaggaa
    12961 tatatcccta acagcgtaga aatggcagaa gacgaaatga tcttgctgat tacaggaccg
    13021 aatatgtctg gtaaaagtac gtatatgcga cagttggcat taactgttct gatggcgcaa
    13081 atgggctgtt tcgttccggc aaaacaagca gttttaccta tttttgatcg gatttttacc
    13141 cgaattggtg caagtgacga cttaattgct ggacaaagta cttttatggt agagatgatg
    13201 gaagccaatc aagccctgcg ttatgctacg ccaaacagtt tgattttgtt tgacgaacta
    13261 gggcgaggaa cagcaaccta tgacggaatg gcgttggccc aagcaatcat cgaatatatc
    13321 catcgtcatg tacaagcaaa aacactcttt tctactcatt atcatgaact gacggtactt
    13381 gagaaagaac ttccgcagct taaaaatgtc catgttggag ccgttgaaaa agacggagaa
    13441 gttgtcttct tgcataaaat gatggatgga cctgcggata aaagctacgg catacatgtg
    13501 gcaaagatcg caggattgcc ttccaatcta ctagaacgag cagcagatat cctgcatacg
    13561 ctagaaaatg gcgataacgg acaccatact gttccagctg aagtagaaga gaaaactaaa
    13621 acagccaaac aggagcaaat aaaaccacct gcagaaaaag taaatcaaga ccatatacaa
    13681 gaccgtgtaa aagaagaaaa ccaacagctt tctttattca gtgaattatc cgaaaatgaa
    13741 acagaagtca ttgaatcact gaagcaaata aatttactag aaatgacgcc aatggatgca
    13801 ctaaacacat tatatgaatt acaaaaacaa ctgtaggaag aaggcggata tcgatggcca
    13861 aaatccaaga attatctgaa cgtttagcta atcagatcgc tgcgggagaa gtagtggagc
    13921 gaccagcttc cgttgtgaaa gaattagtgg aaaatgcaat tgacgccggc agtactcaga
    13981 ttgatatctt agttgaagaa gcgggactgc ggacgatcca agtgattgat aacggagaag
    14041 gcattttggc agacgacgta gagaatgcct tcaagcggca tgcgacaagt aagatccata
    14101 atcgcgacga tctttttcgc atacgaacgc tgggctttcg aggggaagct ttgccaagta
    14161 ttgcctctgt ttctgaaatg actctggaaa cggcgaatca ggaagaaaaa caaggcacct
    14221 atctgtcgct gaaaggcgga gaagttattg agcatcgacc agcaccactg cgtcaaggaa
    14281 cgaagattac cgtcagcaat ctgtttttca atacgccagc ccgcttgaaa tacgtgaaaa
    14341 cactgcacac agaattagcg aacatcgggg acatcgtcaa tcgactggca ttgagtcatc
    14401 catcgattgc ttttcgttta gtacatgatg gaaacaaaat gctggcaact gctggaaatg
    14461 gcgatctgaa acaaacaatt gcgggaatct acggattatc taccgcaaaa aaaatgctga
    14521 aaatcgaagg aaaagatcta gattttgagt tatttggcta tgtttcattg cctgaggtga
    14581 ctcgtgccag taggaattac ttatcgacga tcatcaatgg acgttttatc aagaactttt
    14641 ccttgaataa agcaatcgtt gcaggttatg ggtcaaaatt aatggtcggt cgtttcccta
    14701 ttgctgtatt ggaaatcaaa atggacccgc ttttagtaga tgtaaatgtt catccgacga
    14761 agcaggaagt ccggctgtcg aaggaaaaag aactaacaga gctaattagt aacgccattc
    14821 aaaaagcatt gtctcaagaa aatctgattc caagtgcagc ggataatcta cggtttaaga
    14881 aaaaagtacc tgatcagcct aaaacagaac aaatcgaaat cgatttgtcc tacgcttttg
    14941 aagaagaaaa acctaaaaag ccaagtagtt tgaattttga tcgagagaca ggtacctttt
    15001 tcgtagaaga accaaagaac acatttgttg acagaattgt tgataagtct gtggaaaatg
    15061 ttgaaaacta ttctggtttt tctgaagaaa acaccactta tccagaaaaa acagcgaaaa
    15121 cggtggataa gtttggtgaa aatgttgata actttgtaga aaaaacagtg gaaaaatctg
    15181 aaaagaaaga atttcccgtt gtggataaca tggagaaaac aaatgaagaa caagcagata
    15241 aatccgtaga agaggatgaa aagatttacg aggaagaatt gacactccat cctgaatttg
    15301 atttcactca aggaaacgac aaaacattag aacaagccat caagaaatta gaaacggaaa
    15361 aaccagtaca acgttttcct cagttggaat attttggaca gatgcacgga acgtatcttt
    15421 ttgcccaaag tagcgatggg ttgtacatca ttgatcagca tgctgcccaa gagcgcatta
    15481 agtatgaata tttccgggaa aaaattgggg aagtatctga tgatctgcaa gaattgcttg
    15541 ttccatttgt gttagactat ccaaacagtg atgctttgaa attaaaagag caaaaacact
    15601 tgctagaaga tgcagggatt tatttagaag aatttggtca aaacagcttt attgtacggg
    15661 cccatcctac ttggtatccc aatggagaag aagaatcgat tattcgggag atgatcgata
    15721 tgctgctgac aactggaagt gtgagtgtaa aaaaattccg tgaagcaaca gcaatcatga
    15781 tgagttgcaa acgatcgatt aaagccaacc attatttgaa tgaagcacaa gcacgtgtgc
    15841 tgctgaagga tctggcacga tgcgaaaatc ctttcaattg tccccatggc cgtccagtat
    15901 tgattcactt taccaattcg gatatggaaa ggatgttcaa acgaatccaa gacccgcatt
    15961 aagtcaaaga gaagagggaa aacatgaaga ttgtattagc atcccaatca ccaagaagaa
    16021 aagagttatt ggctcatatc gtttcagaat tcgagattga acctgctgat atcgatgaaa
    16081 cgccatttcc agacgaacag cccgtcgatt atgttcgtag aatggctgaa gaaaaagcac
    16141 gaattgtctg ggaacaagat gaaaaagaag accatttagt gattgccagt gacacgactg
    16201 ttgttctcaa tcaagagatc atgggtaaac cagaagatct ggctgaggct gaattcatgc
    16261 tgaaaaaact aagcggggaa acacatactg tatatacagc tgttgtttta aaaacaaagg
    16321 aacgagaaga acggatacta gcagaagcac atgtcacttt ttatcctttg acagacgaag
    16381 agatcaaacg ctacttggag acgggggatt atgcggataa ggcaggggca tacggcatcc
    16441 aaaatgctgc gagtgtattc gtcaaagaaa tttctggaga ttattacagt atcgtaggat
    16501 ttccgattgg tgctgtcaat caggcactga aaacattcat ttaaacaaaa aagcggacta
    16561 tctgattttc gattgaaaat ccatgatagc ccgttttttt tattgaaaag attacttatc
    16621 tactctgact aa
//
LOCUS       DAXWLH010000054        16470 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXWLH010000054 DAXWLH010000000
VERSION     DAXWLH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.054"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(397..1053)
                     /locus_tag="ACGE36_001319"
     CDS             complement(397..1053)
                     /locus_tag="ACGE36_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8383720.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1246..1524
                     /locus_tag="ACGE36_001320"
     CDS             1246..1524
                     /locus_tag="ACGE36_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383721.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1543..2751
                     /locus_tag="ACGE36_001321"
     CDS             1543..2751
                     /locus_tag="ACGE36_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8383722.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2899..3264)
                     /locus_tag="ACGE36_001322"
     CDS             complement(2899..3264)
                     /locus_tag="ACGE36_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFF8383723.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3411..4184)
                     /locus_tag="ACGE36_001323"
     CDS             complement(3411..4184)
                     /locus_tag="ACGE36_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8383724.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4358..5218)
                     /locus_tag="ACGE36_001324"
     CDS             complement(4358..5218)
                     /locus_tag="ACGE36_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8383725.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5218..6636)
                     /locus_tag="ACGE36_001325"
     CDS             complement(5218..6636)
                     /locus_tag="ACGE36_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8383726.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6693..7142)
                     /locus_tag="ACGE36_001326"
     CDS             complement(6693..7142)
                     /locus_tag="ACGE36_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8383727.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7587..7850)
                     /locus_tag="ACGE36_001327"
     CDS             complement(7587..7850)
                     /locus_tag="ACGE36_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFF8383728.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7949..9244)
                     /gene="rho"
                     /locus_tag="ACGE36_001328"
     CDS             complement(7949..9244)
                     /gene="rho"
                     /locus_tag="ACGE36_001328"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFF8383729.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9241..10512)
                     /locus_tag="ACGE36_001329"
     CDS             complement(9241..10512)
                     /locus_tag="ACGE36_001329"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8383730.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10677..11546)
                     /locus_tag="ACGE36_001330"
     CDS             complement(10677..11546)
                     /locus_tag="ACGE36_001330"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFF8383731.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            11649..11861
                     /locus_tag="ACGE36_001331"
     CDS             11649..11861
                     /locus_tag="ACGE36_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383732.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(11838..13448)
                     /locus_tag="ACGE36_001332"
     CDS             complement(11838..13448)
                     /locus_tag="ACGE36_001332"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFF8383733.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13670..13915)
                     /locus_tag="ACGE36_001333"
     CDS             complement(13670..13915)
                     /locus_tag="ACGE36_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383734.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(14641..15981)
                     /gene="glnA"
                     /locus_tag="ACGE36_001334"
     CDS             complement(14641..15981)
                     /gene="glnA"
                     /locus_tag="ACGE36_001334"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFF8383735.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(16009..16401)
                     /locus_tag="ACGE36_001335"
     CDS             complement(16009..16401)
                     /locus_tag="ACGE36_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8383736.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4691 a         3510 c         2811 g         5458 t
ORIGIN      
        1 cgtgtcacag ccccttttgt tgcaaataaa cggcgttcaa aagcttacat ctacggtgat
       61 aagtccagcc aagttaaaaa cataagagac tgtttttact tggctcttct tattacttga
      121 tgcagaacag ctttttcaca acctcttggt aaacggtgtt ctcctaccaa ctcctcggtc
      181 acaagtcaca atattcatca aacatgaaaa gctgtttttt gaaattgttc cttgtgactc
      241 agagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
      301 tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa
      421 caagtccata ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt
      481 cagttgtttt aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat
      541 ttcttcattc gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa
      601 atcatataat tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg
      661 ctgcattttt ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg
      721 tagctttggg tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc
      781 tgcatattct gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc
      841 ttttaaaaga gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc
      901 atttgaaata acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc
      961 catcccttcg aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt
     1021 atccaaatat tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc
     1081 taaaaagatc atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata
     1141 ttaacatttg ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc
     1201 gttatactgc agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg
     1261 aacacaatca ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct
     1321 ttagtctttt taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag
     1381 aatccaacaa acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt
     1441 attcgccaac ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa
     1501 aaattaaaag catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt
     1561 aataaatatt ttattggaat cacattgatc aattttattg tctacctgat ttattatctg
     1621 ttgatggtca tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca
     1681 ggttttgctt ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa
     1741 cagttagaac tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt
     1801 tcaacattag catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg
     1861 aatggattcg gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc
     1921 cctgcatcta aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc
     1981 gctgttgggc catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc
     2041 tggttttcaa ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa
     2101 aatattccat tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt
     2161 attgaaaaga aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca
     2221 agtgttttat catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca
     2281 tttttcttcg tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt
     2341 ttcgatgtca aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga
     2401 ttatttttac tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc
     2461 ggcctaggtt atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc
     2521 gaacatcgaa tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc
     2581 gttggtcctt atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg
     2641 attgccggag tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat
     2701 agtcatgaag cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga
     2761 atagataaaa aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata
     2821 gtttatgatt attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag
     2881 aatagtttaa ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag
     2941 caaccacttg tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt
     3001 catccaaata tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc
     3061 tgaaccagca tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc
     3121 ccctagcaca tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct
     3181 aacaccgtat aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac
     3241 taccaacgcc ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga
     3301 ttatctaaca agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat
     3361 aaggtcctat ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac
     3421 agacattcga tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt
     3481 gtcaccagat aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt
     3541 ttttgctgtg taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc
     3601 attgcctctc cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc
     3661 attatcgcta aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt
     3721 gttgctcgtt tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca
     3781 ctcaacatag cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct
     3841 aggtttctga ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct
     3901 cgaatcaaag aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg
     3961 gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg
     4021 cgaactctac ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct
     4081 tcgctataat ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga
     4141 taattcacaa tctcctgctg tctattctcc acttcattca acataaaatc accccattta
     4201 ctttatatag taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag
     4261 aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag
     4321 actattgtca taccctcttg accacacatt tttattttta gtataaacta gccttgccta
     4381 ctgtgttcaa aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg
     4441 gataaattgc attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat
     4501 tgaaaaagac gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg
     4561 cttcgctgac ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga
     4621 tttgttggtt caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat
     4681 acaagccatg cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa
     4741 aaatcttcgc ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac
     4801 cattattccc aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg
     4861 caaccacttt tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca
     4921 tcacggaaga atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat
     4981 ccaagtggat aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa
     5041 actcatcact taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta
     5101 atctctctgc ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg
     5161 gtactgcaaa ttgattctct ttagccactt tcaataactc tttcattgta tataacatta
     5221 cgcttccacc tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt
     5281 cactgttttt ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc
     5341 caagacatac ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc
     5401 atgaggtgcc attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc
     5461 tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc
     5521 tgtaatatac gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt
     5581 aaatttatgt ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc
     5641 agccatacaa gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt
     5701 cgaagtcgat ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa
     5761 tccgaacaat agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa
     5821 cacccatgtg atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga
     5881 taaaaagggg acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt
     5941 atttgccaga aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa
     6001 acctgaacca cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt
     6061 tccagcaatc gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt
     6121 atctcctccg atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc
     6181 agccgctgta atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa
     6241 tggaagcatg taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt
     6301 tgcagctggt tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc
     6361 tattactact tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc
     6421 ggcaaatcgg tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt
     6481 gatttcttgt tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg
     6541 atggtttaga tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat
     6601 tccagtcggg caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca
     6661 ctatcgatac tcggacaaag actctactat tttcattctt gactaatctg tctgatgata
     6721 tctgctggcg tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg
     6781 gagagtgtgc tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca
     6841 aaaatatatt caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga
     6901 aaaaaacaca gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca
     6961 tcttctattc ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga
     7021 acatttctta ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct
     7081 tttgaatcta tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc
     7141 ataaaaaacc tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag
     7201 catcgctagc tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc
     7261 gtgtataagt cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa
     7321 caaaaagcct tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact
     7381 tcgaaataac ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa
     7441 actatatact agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa
     7501 acgaagtttt aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc
     7561 taaacgttga gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat
     7621 tttttgttga aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag
     7681 aatggatgtg agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc
     7741 cattcaactg tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt
     7801 gaatccatga atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg
     7861 ctcctttgcc ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca
     7921 tcttaacatg tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt
     7981 taccaaagga gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca
     8041 aaaagcggat cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt
     8101 tccaaatttc ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt
     8161 ttttgatatc gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt
     8221 ctagatttcc agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat
     8281 cgactaaagc ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc
     8341 cgaaaaattt cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc
     8401 cgcttggtgg aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga
     8461 taacgacatc tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa
     8521 ctcgagtatg gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc
     8581 ttcgttctag atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta
     8641 cttccggata gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg
     8701 cttttggcgg tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga
     8761 tcattcgggt agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa
     8821 gaggtgttaa ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa
     8881 cgctttccac gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc
     8941 ctgccacttt gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga
     9001 tatcttctgc acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa
     9061 cgatatccaa tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca
     9121 cagctagaga taattccttt ttattcatct tactgtaata aggaatttta aattctttgg
     9181 cataactata aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc
     9241 tcattcagct cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc
     9301 gataatatga tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc
     9361 tgccaatagt ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc
     9421 tgtgccgtga agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc
     9481 agcgcccatt cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat
     9541 gatttctgaa gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt
     9601 ggcaaatcct ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta
     9661 aacttcgatt tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat
     9721 aaagctttcc aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc
     9781 tgctaatgct agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc
     9841 atgtaatgtt tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc
     9901 ccccatatta ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc
     9961 aattactgtc tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac
    10021 tgaaaccacg tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata
    10081 catggcacca tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag
    10141 gtcaatcggt ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa
    10201 tttaccaagc agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg
    10261 cggcatcgga atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg
    10321 gacagttgcg cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg
    10381 cacaccttcc aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc
    10441 actatttttt gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac
    10501 gatttttttc atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt
    10561 ttatcataga taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga
    10621 gagcgacttc aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac
    10681 gctttgttag ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct
    10741 ttacctggag ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct
    10801 tcgatatatt tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact
    10861 tttgagatac ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca
    10921 tgtaatacta aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa
    10981 gctaatcctt tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag
    11041 tagtcgatac ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta
    11101 ccgatgattc cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct
    11161 tttgcatgtg ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga
    11221 tgtgaaccat cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg
    11281 taatcaccat ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa
    11341 tcagtgatta aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat
    11401 gtttggataa gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat
    11461 tccaagttgt ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa
    11521 aattcagctc ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat
    11581 ttgttaagac aattcttaac caacgaccct attttaccaa acatttcggt agttttctac
    11641 taaaatcaat gaaaatgaaa attttcaaga agttttttgc aaaaaataaa atatccaata
    11701 gcgctttaat cgaaaaaagt cttttgcttt ctatcttttt tcctacaaat aataacaaat
    11761 cagaaaaaga gaaaagtgac aagaaaattg cttatcccct tctcttgcta cttttctctt
    11821 tctatcaagt tcaaaaatta ttttgtttcg tgatctgcta atgatgctcc gacaaatgct
    11881 ttgattaatt tttgcgggcg atttggacga gaaatcaatt ctgggtggaa ttggcatccc
    11941 acaaagaatt ttttctctgg cagctctacg atttctacta aacggttatc tggtgagaca
    12001 cctgaaaaga ctagcccttg ttcttcaaac aattgacgat atttgttatt gaactcatag
    12061 cggtgacggt gacgttcttg cacaacatcg gctccatcat atgcttcagc cgtcttcgtt
    12121 ccttttttga ttttacatgg gtataatcca aggcgtaaag ttccacccaa attctcgatg
    12181 ttttcctgat cagccattaa atcaataata ttgtttgctg tatctggatt cgtttcagct
    12241 gatgacgcat cttctaatcc aacgacgtta cgtgcaaatt cgacacaagc catctgcata
    12301 ccaagacaaa tacctaaaaa cggaacgtca ttttcgcgag cataacgaat cgcttcgatt
    12361 ttcccttcta ctccgcgatc accaaatcca ccaggaacca agattccatc tgcgtctttc
    12421 aaacgttctt ctacattttc tctcgttaat tcttgtgctt tgatccaatc gatctcgatg
    12481 tctgaattat aggcgaaacc agaatgtttc aatgcttcaa ctactgagat atatgcatct
    12541 ggtaactcga catatttacc gacaagcgca atccgtactt ttttcttcaa gttcaagaca
    12601 cgttcttcta attctttcca ttctgtcatg tctgcttctg gtgtatctag ttttaaatgg
    12661 tcgcaaacaa tttgatccat tccttgtgct tgtaaaacta atgggatgga gtaaagtgta
    12721 tctacatctg gcgactcgat aactgcttct ggttcgacat cacaaaattg tgccaatttg
    12781 tttttcacgc tttgagaaac aggttgctct gtacgtacca ccaaaatatt cggttggata
    12841 cctaaaccac gtagctcttt tacactgtgc tgcgttggtt tagttttcat ttctccagca
    12901 gctttcaagt aaggaatcaa tgttgtatgg atatacataa cattatctgt accgacatct
    12961 gctttcattt gacgaagtgc ttcaaggaat ggcaaagatt ctatatcccc tactgttccc
    13021 cctacttctg taatgatcac atctgaatca gtcattttag ctgctcgcat gattttttct
    13081 ttgatctcat ttgtaatatg aggaataact tgaacagttg ctcccaagta ttcgccctta
    13141 cgttctttac gcaaaacttc agaatagatt tttcctgtcg tcacgttgga gtatttgttc
    13201 aaattgatgt caataaaacg ttcgtagtgg cccagatcca aatctgtttc agcaccatca
    13261 tctgttacga atacttctcc gtgctgatat gggctcatcg tccccggatc cacgttgatg
    13321 tatggatcaa atttttggat agttactttt aagccgcggt ttttcaataa gcgtcctaaa
    13381 gaagctgcaa caatcccttt tccgattgat gaaacgacac cgccggtcac aaaaatatat
    13441 tttgtcataa gttcaaaaac tcctttttaa tttatgtgca ggaaaagtct cagacaataa
    13501 acaaagctcc ctattcttca ataagaatag ggagccgaaa tttctgtaaa cttctgaccc
    13561 tctaaaaggg tgcccaaggt gtataataca agctgactaa gggaaagtca agtactcaaa
    13621 tgaatactat ccgcttacaa ttttttcttt ttcgatatcc tttgagaaat tatttcttaa
    13681 gtcctttatg agtgcaaaaa aaatttaacc cacataataa gcttgatata aacatagcta
    13741 acgttaaacc taagcaaatc agaaaatcgt tactgaaaaa ttttaagtga aataatagat
    13801 aaaaaaataa aacaaacatt atgatagata atatcccacc taacaaagaa ctactttttc
    13861 ctacttgcag caacagttta tatagcttat cgtagaaaca atctttttct ttcaccctta
    13921 tttacctcct ttacgtaaaa aacgtagtcc acagttctcg gatttttggc atatcttttc
    13981 tattaaataa tatcaaaaac aaaacaattc cactaatttt ttcaaagatg accttttttt
    14041 caacaaaagt gccttgtagg gattcattga aggtaacgaa tcacataagt aaaaaataag
    14101 gagatgcgac aaaagtcgtg cctccttatt tctaaataaa cggtgttctc ctgccaactc
    14161 ctcggtcaca agtcacaata tccatcaaac atgagaagct gtttttgaaa ttatttcttg
    14221 tgattcggag ttaaatggta ggctcacaac ctctggataa acggtattca aaagccagtt
    14281 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
    14341 taatactcga aactaaacgt ctttttcaaa acctcttggt aaacggtgtt caaaagcttg
    14401 catctgcggt aataagttca gccaattcaa aaacaagaaa agctgttttt gcttggctat
    14461 tcttattact tgttgcaggg ctgctttttc acaacctctc cactaaacgg tgttcaatca
    14521 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgttagt
    14581 ttctctaata ctcaggtcaa aacgctgatt tcacagcctc taattcacgg tattcctgtt
    14641 ttaataaagt tctagatatt gttctctttc ccactcggaa acagtttgtc tgaatgccgc
    14701 ccattcgaga cgttttgctt caacaaaatt tgagaagata tgttgtccta atgcgtcaat
    14761 cattacatta tccttacgca attctttgat agcattatgg atagttgaag gtaagtcctg
    14821 gatatgtgca gcttctcttt cttcctcatc cattacataa atgttacggt caacagctgc
    14881 tggcggtgtg atctgattac ggataccgtc tagtcctgct tgcaacaata ctgccattgc
    14941 caagtatggg tttgcagatg gatcaactga ccgcaattcc aaacgtgtgg ataaaccgcg
    15001 agattctggt acacgtacta atggcgaacg attacggcca ctccatgcga cataaacagg
    15061 cgcttcataa cctggaacga gacgtttata agaattgact gttggattgc aaacggctgt
    15121 gtaggcacga gcatgtttca gcaaaccacc taagaaatga taagctgttt cacttagttc
    15181 caattcaccg tctttatcat agaaaacatt tccttcatca ttgaacaaag acatattgca
    15241 atgcatacca gaaccattga tcccaaataa cggtttcggc atgaaagtcg cgtgaagtcc
    15301 atgcttgcgt gcaatcgttt tgacaacgag cttgaaggtt tggatattat cacaagcatc
    15361 taccacatct gcatatttaa agtcaatttc atgctgtcct ggcgctactt catgatgtga
    15421 agcttccact tcaaagccta gactttctaa ttccaacacg atgtcacggc ggcaattttc
    15481 acctaaatct gttggtgcaa aatcaaaata accaccttta tcattcaagg tcgttgtgat
    15541 cccaccgtct tcgtctaatt taaataagaa gaattctggt tcaggtccta aattgaaaga
    15601 ggtaaaacca aggtcgcgca tatctttcaa tgctcgtttc aaattcccgc gaggatcgcc
    15661 ggcaaatggt gtcccatccg gattatagat atcacaaatt aaacgagcaa cttttccatg
    15721 agcactttcc cacgggaaga tcatccaagt ggagagatct ggatacaaat acatatcgct
    15781 ttcttcaatt cgaacaaatc cttcaatcga agaaccgtca aacatcattt tattttctaa
    15841 tactttgtcg atctggctca ctggaacttc tacatttttg atcgtaccta agatgtctgt
    15901 gaacatcaat cgtaaaaatc gtacgttttc ttcctcaatg attcgtttga tttcttcacc
    15961 tgttatctgt ttctttacca tggtatccct cttccttttt taatttattc agtcaaagaa
    16021 ttgtctttgc aatccttgcg actgataatg acctgttttg cttaacccac cttgcgccaa
    16081 aatctcatca cgaaaaattt gtcgaacgtc atgatctgtc aacggcttct tgttttttgc
    16141 ttcttgtttt ttcgcctgtg ccatttcgta tacacgtttg atcccagcca tattcaatcc
    16201 ctctgaaaga tagtctttaa tctcaagcaa catatcaata tcattcagcg aatacatacg
    16261 tcggtttcct tcgcttcgtt ccggatgaac taaatcttgt tcttcataat agcgaatttg
    16321 ccgagcagac aaatcagtca gcttcataac tgtaccaatt ggaaatacgg acatagatcg
    16381 gcgcaattcc ttttctccca tcagacgtcc cctttcgttt tataagttgg ctaaagtgta
    16441 ccaattaaaa aaaatattgt caactgcata
//
LOCUS       DAXWLH010000055        16304 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXWLH010000055 DAXWLH010000000
VERSION     DAXWLH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.055"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            42..638
                     /locus_tag="ACGE36_001336"
     CDS             42..638
                     /locus_tag="ACGE36_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFF8383737.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            619..1065
                     /locus_tag="ACGE36_001337"
     CDS             619..1065
                     /locus_tag="ACGE36_001337"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8383738.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1408..1731
                     /gene="relB"
                     /locus_tag="ACGE36_001338"
     CDS             1408..1731
                     /gene="relB"
                     /locus_tag="ACGE36_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8383739.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2051..2278
                     /locus_tag="ACGE36_001339"
     CDS             2051..2278
                     /locus_tag="ACGE36_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFF8383740.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2294..3460
                     /gene="hemW"
                     /locus_tag="ACGE36_001340"
     CDS             2294..3460
                     /gene="hemW"
                     /locus_tag="ACGE36_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFF8383741.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3695..4738
                     /gene="hrcA"
                     /locus_tag="ACGE36_001341"
     CDS             3695..4738
                     /gene="hrcA"
                     /locus_tag="ACGE36_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFF8383742.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4832..5395
                     /gene="grpE"
                     /locus_tag="ACGE36_001342"
     CDS             4832..5395
                     /gene="grpE"
                     /locus_tag="ACGE36_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFF8383743.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5446..7275
                     /gene="dnaK"
                     /locus_tag="ACGE36_001343"
     CDS             5446..7275
                     /gene="dnaK"
                     /locus_tag="ACGE36_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFF8383744.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7426..8592
                     /gene="dnaJ"
                     /locus_tag="ACGE36_001344"
     CDS             7426..8592
                     /gene="dnaJ"
                     /locus_tag="ACGE36_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFF8383745.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8960..9697)
                     /locus_tag="ACGE36_001345"
     CDS             complement(8960..9697)
                     /locus_tag="ACGE36_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8383746.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9699..11201)
                     /locus_tag="ACGE36_001346"
     CDS             complement(9699..11201)
                     /locus_tag="ACGE36_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8383747.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11460..13454
                     /gene="uvrB"
                     /locus_tag="ACGE36_001347"
     CDS             11460..13454
                     /gene="uvrB"
                     /locus_tag="ACGE36_001347"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFF8383748.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13464..16280
                     /gene="uvrA"
                     /locus_tag="ACGE36_001348"
     CDS             13464..16280
                     /gene="uvrA"
                     /locus_tag="ACGE36_001348"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFF8383749.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
BASE COUNT         5435 a         2806 c         3533 g         4530 t
ORIGIN      
        1 tctaatgcaa ttatattgat atcattagga gctgcattgt tatgaatcca acattgatta
       61 ctaagaaaga attactaaaa aaattagata tttctactgg tgtattagct aatttaatta
      121 gaaatggcat gccaaaagag ggagaaatgt ttaatcttga taagattatt acatggcgag
      181 aaaattggtc taaaaacata ttaggtgagt tagaagttgg tagagtatat actaacaaag
      241 aaatttcaga gaagtttaaa tgttccaaac agggtggaat gagaagatct catcaaacta
      301 atactcttgt actgtttagt gatcaaacag gaagtaatgt ttataaagat aagtggttaa
      361 atggtatttt acaatatacc ggtatggggc taaaagggga tcaagtattg gataagaatc
      421 aaaataaagt tttagccaat tcaaaaagta attttgtaaa gattcattta tttgaaacat
      481 ttaaaccaaa agaacacaca tatctgggag aagtatattt agcaggacaa atatatactg
      541 ttaatgaaaa agatagttcg ggcaacagca gaaaagtata taaatttcca ttagctttaa
      601 taaaccaaga gcaattgatt gaagataaag atatataacc aggaagaaaa tcaaactaga
      661 catattcgta atttatctaa tgctaaatta gaggaagaag caagaaaagt tagtaattat
      721 aataaaaaaa tggctgcaaa atatgttaac gaagaggagt tttgcttctc aaaaacaaaa
      781 atatataaac gtaatccctt cgtatctgaa tatgttaaac gacttgcaaa aggtatttgt
      841 caactttgcc aagaaaaagg gccatttata aaagacggtg taccttattt acattgtcat
      901 catatagaat atttgtcgca aggtggaaaa gatgtgattg aaaactgtat cgcgttatgt
      961 cccaattgtc atgctagaat tcatgaactt gagttacctg acgataaaga aaaattgctg
     1021 aagatcgttg aaaaaagaga aaatagcttt tctaaaatac cgtaatttat ttttatagga
     1081 caaataagct aatgtgagct atgccttcta tataagacta agttctatta taagtaacga
     1141 agtaattctc gttccttcct gtagtaatat tttttaaata acctataggc ttcttcaaaa
     1201 gtacatcctc cagtaaattc taagtactgg aggattcttt ttctagtgtt gctttactca
     1261 tgaaaaagta gttggctact tcatcaaaat ttctgaactt ttttaatgta taataaagag
     1321 ctcgatcatt tactacaagg atgctagctc ctacattttc acagtttttt agcaagtaaa
     1381 tttttctaaa atcacatcag gaaatgcatg agattcttta agagaggcaa ttatagggct
     1441 atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc tttcttatat ggctgaattt
     1501 tatgggatta ctttatctga acttctaaaa aactattcga tggaacaact agaagatgaa
     1561 tacaatcgtc aaacggctga aattgcccat aaacattgga taagaagtgg gaaaacaaca
     1621 gtattcatgg aagaatcctt aatgaatttg gtgggctgga atgacctgcc gtttaaaatt
     1681 cacgcccgaa attcagaagc agctctgatt ggcaaatacg tgtattggta gagatagaag
     1741 ataatcaatt cgttgttatc gctattcaag tagggcaccg aagaaatgtc tatgattaag
     1801 agactgttta tttttacaaa aaaataataa ttggaatcta gttaaaactc ttgaagtgag
     1861 agatttgact agattttttt atgaataaaa taaggacaaa attgtttagg aagaatcatg
     1921 agtgtaaaaa aatcatccaa gaagtcgatt cttggatttt ttagtggatt tgttcgccaa
     1981 atacgtttag tatgcgtata tgagaggttg taagcaaagc gttgtggtct gagcacttaa
     2041 ggaggaatct gtgaaaatag cttttcatat ttttgcagat tttgacttaa tgccgaaggt
     2101 cagctttgcg attaccgttt atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa
     2161 taagcaagca tttttagagc taggtttcct tgattctaaa tatgcggctt atttcaatga
     2221 gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg
     2281 agaaagaatt tcaatgatag aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat
     2341 tcatatccca ttttgcgagc atatctgcta ttactgtgac tttaataaag tttttttaga
     2401 agggcagcca gtagatgagt atgtggaaat gctgctgaat gaaatgagat taatgctgga
     2461 aagtcatcct acagcgaatt tggaaacact ttatgtagga ggcggaacac caacttcttt
     2521 atctgctaaa caacttgatc gactattatc aggtgctaga gagatcctgc cttttcaaaa
     2581 tggtaatgag tttactgtag aagcgaatcc tggtgatttg acccgtgaaa aacttcaagt
     2641 gatgaaaaac tatggcgtca atcgtttatc catgggagtg cagacatttg acaatcgctt
     2701 attgaaaaaa attggacgta aacatactgc agaagatgtg tatcaaacaa tgcaattttt
     2761 agaagcagaa aattttacca atgtaagcat cgatttgatt tatgcattac cgggacaaac
     2821 actagaagga tatcgggata ctttggaaaa agctttggct ttggatctgc ctcactattc
     2881 tttgtactca ttgatcctcg aaaacaaaac aatgttcatg aactgggtac gacaaggaag
     2941 actcgaactg cctgatcagg aaacggaaac cagaatgttt gaagagacga ttgaagcaat
     3001 ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt gggctctccg gacatgaatc
     3061 tcaacataac ctcatgtact ggaacaatga ccactatttt gggtttggag ctggtgctag
     3121 cggttatctt ggagatattc gttaccgcaa caaaggacca atccaacact atttacgccc
     3181 attacgagca aatgaacgcc cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat
     3241 agaagaagaa atgttcttag gactaagaaa aaaaacaggg attactaaaa aacatttcta
     3301 tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat gtgctaaggg aattagagca
     3361 agaagggtta cttgttaatg aagaagaccg tgtttatttg acaccaagag ggactttttt
     3421 ggggaacaat gtgtttgagc gctttttatt tgaaaaatag tttgaagaat atttgttttt
     3481 tatcgaaact tgaaaagaaa aagtttagaa agtttgacaa tgaggtattg aagtattgat
     3541 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
     3601 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg aattagcact
     3661 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
     3721 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
     3781 aatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
     3841 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
     3901 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
     3961 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
     4021 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
     4081 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
     4141 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
     4201 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
     4261 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
     4321 gacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagaaaa
     4381 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
     4441 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
     4501 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
     4561 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
     4621 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
     4681 agaactagca gctaaacttg ctgattacta ccgctcgctt gacttgtcaa actcgtaatg
     4741 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
     4801 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
     4861 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
     4921 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
     4981 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
     5041 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
     5101 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
     5161 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
     5221 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
     5281 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
     5341 ctacaaatta catgatcgcg tgttaagacc aactatggtc attgttgcac aataattgtg
     5401 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
     5461 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
     5521 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
     5581 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
     5641 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
     5701 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
     5761 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
     5821 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
     5881 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
     5941 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
     6001 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
     6061 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
     6121 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
     6181 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
     6241 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
     6301 cgtacaaaag taccagtacg tcaagcgtta aaagatgcag gtctgtcaca atcagaaatc
     6361 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
     6421 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
     6481 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
     6541 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
     6601 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
     6661 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
     6721 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
     6781 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
     6841 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
     6901 atggtgaaag atgcagaagc aaacgctgaa gcagataaag ctcgtaaaga ggaagttgac
     6961 ctacgtaatg atgtagacgc attgctattc tctgtggaca aaacattgaa agaattagaa
     7021 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgtgacga attaaaagcc
     7081 gctgttgaag caaacaacat cgaggaaatg aaaactaaac gcgatgcatt gaacgaaatc
     7141 gttcaaaact tgactgttaa actatacgaa caagctgcac aacaacaagc tcaagaaaat
     7201 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
     7261 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
     7321 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
     7381 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
     7441 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
     7501 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
     7561 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
     7621 tacgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
     7681 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
     7741 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
     7801 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
     7861 atcaagtata atcgggaaga aatctgtcat acttgtggtg gaaacggtgc aaaaccaggc
     7921 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgatcaatgt agaacgccaa
     7981 acgccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg cacagggaaa
     8041 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
     8101 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
     8161 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtttt tcgtgtagaa
     8221 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
     8281 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
     8341 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
     8401 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
     8461 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
     8521 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
     8581 aagcggaagt agtttttcta gtaaaaatat aaagtagact tgcccaaatt ttctatttct
     8641 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatac gttcgcctgg
     8701 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat aagataatca
     8761 tcatagtttc tgaaggtggt atcgactatg tacttgatat cgtcaatgaa ccgatggtac
     8821 tttcttatgc gatggaacag gtagagcgat tgcttgtttg tgcaggagaa caagcgatgc
     8881 ggctggtaca gttaagtgaa aaaacaagaa actaaaaatg cccggtcgtc atgaagcgac
     8941 cgggcatttt tggtttttat taaatattta atactttatt caagaaatct tttgtccgtt
     9001 cattttgcgg attttcaaag atttgttgag gcgttccgtc ttctaaaaag tttccgtcgt
     9061 caatgaacat gacacgattg gccacttctt tagcgaagcc catttcgtgc gtaacgataa
     9121 ccatcgtcat accttgttct gctaactttt tcatgacgtt caagacatca ccgaccattt
     9181 cggggtcgag tgcagatgta ggctcatcaa aaagcatgat gtcagggttc atcgctagag
     9241 cacgagcaat agccacacgc tgcttttgtc caccggaaag catttcagga tacatatctt
     9301 ttttatcttc tagaccaact gtctgcagca atttgattgc ttttttctct gcttcttctc
     9361 tagattcttt cttcaggtcc atcggtgcta aaatgatgtt ttccaaaata gaaagatgag
     9421 ggaataggtt aaagtgctgg aataccattc cgatatgttg tctcaccaag ttgatatctg
     9481 tgcttttatc catcaagtgg gcgccgtcaa taataatttc accgtcagaa ggttcttcta
     9541 aacgattcaa gcaacgcaaa aatgtacttt ttcctgaacc tgaagggcca atgatacaga
     9601 cgacatctcc ttcgttgatg gacacattga tgtcgtttaa tactgtgtta tcgccgtatt
     9661 ttttgactaa atgctcaact aagatttttt ctgccatctt acttcacctt cttttctaaa
     9721 acttttgcta attttgtcaa tgctgtgatc aaaatcaagt acatgatcgc aatgatcaag
     9781 taaacatacg tactttgtgt tgtacgtgca acaatgatct taccagtttg cagcaattcg
     9841 actacaccga ttgcggaaat gatcgttgtg tcttttaatg agataacgaa ctgattgacg
     9901 aatgatggga tcatgatgcg tatcgcttgc ggcaaaatga ttttttgcat tgtccgatta
     9961 taagaaagac ctaaacttct ggaagcttcc atttgtccga caggaacggc gttgatcccg
    10021 cctcgaacaa tctctgcgat ataagcactt gcgttaagag tcaaggtgat aacaccagca
    10081 aggaagtcag gaatcgtaaa tccaacgata ccaggcaacc caaagaaaat aaagaacgca
    10141 agaaccatca ttggaattcc tcggatgata tcgacataaa tactagcaat tgtgcgaaga
    10201 gcgcgtattg gtgctacact gaataatcca aaaatcacac cgattactat agccaaagca
    10261 aaggagatca aagttaggag aattgttctc caaagtcctt tcaacaatgc ttgatagttg
    10321 ttttggataa gtccaagaaa tgttgattca tcagcagtgg aagactcact ggaagtttct
    10381 gaaccatccg ctacatattc tttaataatc ttatcgtatt cgcctgttct ttttaattct
    10441 ttcaatcctt cattgaacat attaagaagc tcaggatttt gtcctttttt aacggcaaat
    10501 gcatattttc cgccagattc ttttttgatc ggtgtttcca attcttgtcc ttgtttgatc
    10561 gcataaccga ttaccggata atcatccatc attgcatcga ttgcgccaac ttttaacccg
    10621 tcgtacaatt gatcagcagc atcaaaatat ttgatagaat agccatattc agattcatgg
    10681 tcgcttaaaa attcagcact ttcggtacca acttttgctc cgactgtttt tcctttcaaa
    10741 tcttcataag acttgatcga agtatctcct tttttgacag ctaattggat cccgctatca
    10801 aagtaagaat cagaaaaatc atagcttttc tttcgttcat ctgttacagt cattccggca
    10861 atcattccgt ctgcttgtcc tgattccaaa gcttgcaaag cagcagagaa accacggtag
    10921 tccattcgga tgttaaatcc ttgcagtttg gcgatgtttt ccatcaattt tacatcgatg
    10981 ccttcgtact cgccctttgc gttttgaaat tcaaatggag caaaagcaga gtcactggca
    11041 attacgtata cgtctttttt gggctctatt ttttgcattg tctcgcttgt gcttgactct
    11101 gccgttgtag tgttgtcagc ttgtgcaaca tgtccaaacg caagtgtcac aagtcctgtc
    11161 attaagaaaa caaatgagtg aaataaatgc tttcttttca taatcaacct cctaaatttc
    11221 caatatttta ttattatagg cgaaaagtgc tgaatcagac aagactgtaa gctcgaaaaa
    11281 atgaaaaaaa tgcgagtttc taacatttta tgttataaaa cgtaacatgc ataataagat
    11341 aaaaaagtgg cgaaagtatg ttcggtgtgg tataattttt tagtaaggaa tgcctttgct
    11401 ttccttttct ttttattggt cgtaaaatga aaaggaatga atgaaaagga ggacgaagaa
    11461 tgattgagag agatacctcc cgacactttg agcttgtctc gaaataccaa ccggctggtg
    11521 atcagcctga agcgattaac caattagtgg atggcgtagt cggcgggaaa aaagcacaaa
    11581 tcttattagg tgcgactgga acagggaaaa catatacgat ttccaatctt attgagaaag
    11641 tgaataaacc aacactaatt attgcccata acaaaacatt ggctggtcag ctgtacgggg
    11701 aattcaagga atttttccct aacaatgctg ttgaatattt tgtttcttat tatgattatt
    11761 atcagcctga agcttatgtg ccttcaagcg atacgtatat cgaaaaagat tcaagtgtaa
    11821 atgatgagat cgacaaatta cgacattcgg ctactagttc attattagaa agaaatgatg
    11881 tgatcgtcat tgcatccgtt tcatgtattt ttggattagg ttcgcctttt gagtaccaaa
    11941 aacaagtagt ttcgattcga cagggagcag aactagatcg caatcagttg attcgtgact
    12001 tagtcagtat ccaattcgaa agaaatgata tagattttca gcgcggtcgt tttcgagtgc
    12061 gcggtgatgt cgtggagatt tttccggctt caagagatga acgtgcctta cgtgtggagt
    12121 tttttggaga tgaggtcgaa cgtatccgag aagtcaatgc actgacaggt gaagttttag
    12181 gagaaacgga acacgtggca atcttcccgg cgactcactt tgtgacgaat gatgaacata
    12241 tggagcatgc cgtagcgaat atcaaagcgg aattagaaca gcgtttaact gtcttgcgaa
    12301 atgaaaacaa acttctggaa gctcaacgtt tagaacagcg aaccaattac gatattgaaa
    12361 tgatgttaga aatgggctac acgtcgggaa ttgaaaatta ttctcggcat atggacggac
    12421 gtaaagaagg tgaaccgcct tatacgctat tggatttctt tcctgaagat tttttgatcg
    12481 tggcggacga atcgcacgta accatgcctc aagtcagagg aatgtataat ggtgaccgag
    12541 cgagaaaaca gatgctagta gattacggtt tccgtttgcc atctgcttta gacaatcggc
    12601 cacttcggtt agaagagttt gaaaaacatg tcaatcagat catttatgtc tctgcgacgc
    12661 caggaccata tgaacatgaa caaacagata ccgttatcca acaaatcatc cgtccgacag
    12721 ggctgcttga cccagtgatc gaagttcgtc caatcatggg acaaatcgat gatctagtag
    12781 gcgaaataaa tgaacgagtc gaaaaagacc agcgggtgtt tgtaacgaca ttgacgaaaa
    12841 aaatggcaga agacctcaca gattatttca aagaattagg gatcaaagtc aaatatttgc
    12901 atagtgatat caaaacgctg gaacgaacag aaatcattcg agatcttcga ttaggtgaat
    12961 ttgatgtgtt agttggaatc aatttgcttc gtgaaggatt agacgtacct gaagtttccc
    13021 ttgttgctat cttggatgcg gataaagaag gctttttacg tagtgaacgt tccttagtcc
    13081 aaacgattgg acgagctgct cggaatgaag aaggtaaagt aatcatgtac gcagacaaag
    13141 taacagattc tatgcgtcta gcgatggatg aaacatcaag acgccgtacg attcagcaga
    13201 agtacaatga agaacatggg atcgttccaa aaacgatcat taaagaaatc cgagatttga
    13261 tttctattac gaaagaatcg gaagatgata caaaagaagc ggtacaagtc tcgtatgaag
    13321 aaatgacgaa agaagaaaaa gatacgttat tgatgaaatt agaaaaagaa atgaaaaatg
    13381 cggcgaaagc attggatttt gaaacagccg ctaatgtgcg agatatgatt ttagaattaa
    13441 aagcttcaaa gtaggaggac aacatggcta acgataaaat tacgattcac ggtgcacgtg
    13501 cccataactt gaaaaatata gatgtaacga ttccacgaga taaattcgtg gtcgttacag
    13561 gtttgtccgg ttctggaaaa agttccctag catttgatac gctgtacgct gaagggcagc
    13621 ggagatacgt agagagcctt tctgcttatg ctcgtcagtt tttaggacaa atggataagc
    13681 cggacgtaga tagcatcgac ggattgagtc cggcgatttc tattgatcag aaaacaacaa
    13741 gtaaaaatcc tcggtcgaca gtaggtactg tgacggaaat caatgactat ttacgcttgt
    13801 tgtttgcacg tgtaggacat ccgatttgtc ccaacgatca tatcgagatt accagtcaat
    13861 cagtagatca aatggcagat caagtaatgg ctttgcctga gcgcacaaag atccaagtgt
    13921 tggcaccgat cgtggtaaag aaaaaaggac aacataaaaa aatattcgaa cgaatccaaa
    13981 aagagggata cgtccgcgtt cgagtcgatg gtgagatcta tgatttaagt gaagtgcctg
    14041 agctggagaa aaacaaaaaa catgatatcg cgatcgtcat cgaccggatc attgtgaaag
    14101 aagggattcg ttctcgtcta tttgattctt tagaggcggc tctgcgattg gcagacggct
    14161 atgctctggt tgacgtgatc ggtgaagaag aaatgttgtt tagcgaacac tatgcctgtc
    14221 cttattgcgg atttactgtt ggcgaactag aaccaagact gttctcattc aatgcgcctt
    14281 ttggagcttg tccagactgt gatggtttag gtgtcaaatt agaagtcgat caagatttag
    14341 tgattccaga tagaacgaag acacttagtc aaggtgctat cgttccatgg aatccaatca
    14401 gctctcagta ttatcctcaa atgttagcgc aggcttgtga aagtttcggt atcgatttag
    14461 atacaccatt cgaagaatta ccggaagagc atcaggatat catcttgaat gggacagatg
    14521 atcttttcca tttccattat gaaaatgatt ttggtggcgt tcgcgatgta gaaacgactt
    14581 tcgaaggggt attgaagaat atcgagcgtc gttatcatga tacgaacagt gatttcacgc
    14641 gtgagcaaat gcgtctttat atgacagaat taacttgtca gacatgtaaa ggttatcgat
    14701 tgaatcccca agctctatct gttcaaatca atggaacgaa tatcgggcaa acaaatgagt
    14761 tgtctattca aaaagcagta gatttcttct caaacttaac gttatctgaa caagagaaag
    14821 taattgccaa accgatttta aaagaagtaa atgacagact ttcattcttg gaaaatgtgg
    14881 gactagacta tctaactttg agtcgcgctt ccggaacatt atctggggga gaagcgcagc
    14941 gaatcagatt ggctacacaa attggatcga atctttctgg agttttgtat attttagatg
    15001 aaccatcgat cggattgcat caacgcgaca acgatcgact gattggctca ttaaagaaaa
    15061 tgcgtgatct tggcaacacc ctcgttgtag tcgaacatga tgaagatacg atgtgggcag
    15121 cagattatct gattgacgtc ggacctgggg ccggagaaca aggcggacaa atcgtggctg
    15181 caggcactcc tgaagaagta gctaatgatg aaaattcttt gacagggaaa tatctttcag
    15241 ggaaaaaatc aatccctgta ccaaaagaac gccgtaaagg taatgggaaa gcaatcaaga
    15301 tcactggtgc ttcagaaaac aatctgaaaa atattagtgt tgaatttcca ttaggagaat
    15361 ttgtagcagt cacaggtgtg tctggttctg gaaagagtac attggtcaat agtatcttaa
    15421 agaaatcgtt agcgcaaaaa ttaaataaga attctgctaa gccaggtaaa ttcaagacaa
    15481 tttccggcta cgaaagtatc gaaaagatca tcgatatcga tcaaagccca atcggccgga
    15541 cgccgagaag taatccagcg acttatacaa gtgtatttga tgatatccgt gggttatttg
    15601 ctcaaacgaa cgaggcaaaa atgcggggtt ataagaaagg gcgttttagt ttcaacgtaa
    15661 aaggcggtcg ttgtgaagct tgtcgcgggg atggaattat taagatcgaa atgcactttt
    15721 tgcctgatgt ctatgttcct tgtgaagtat gtcatggcaa acgatataac tctgaaacat
    15781 tagaagtgca ttacaaagga aaaagcattg ctgatatttt ggaaatgaca gtagaagatg
    15841 ctgtagaatt cttcaagcac attccaaaga ttcatcgcaa actgcaaacg attgttgatg
    15901 ttggcttagg ttatgtgacg atggggcaac cagcaacgac attgtccggt ggtgaggcac
    15961 aacggatgaa acttgccagt gaattgcaca aaatctctaa tggaaagaat ttctatatac
    16021 tagatgaacc aacgacagga cttcatagcg atgacatcgc ccgcttgttg catgtattac
    16081 aaagattagt agatgctggt aacacagttt tagtgattga acacaatcta gatgtaatca
    16141 aaacagcaga ttatatcatt gatttaggac cagaaggtgg agaaggtgga ggaacgatcc
    16201 ttgcgactgg aacaccagaa gaaatcatta acgtaaaaga aagttataca ggtcactatt
    16261 tgaaaaaaat aatggtataa agaggataga tagctgagat agtt
//
LOCUS       DAXWLH010000056        15680 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXWLH010000056 DAXWLH010000000
VERSION     DAXWLH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.056"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            62..1363
                     /gene="murA"
                     /locus_tag="ACGE36_001349"
     CDS             62..1363
                     /gene="murA"
                     /locus_tag="ACGE36_001349"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8383750.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1376..1552
                     /locus_tag="ACGE36_001350"
     CDS             1376..1552
                     /locus_tag="ACGE36_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8383751.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1687..1983
                     /gene="yidD"
                     /locus_tag="ACGE36_001351"
     CDS             1687..1983
                     /gene="yidD"
                     /locus_tag="ACGE36_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFF8383752.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2168..2635
                     /locus_tag="ACGE36_001352"
     CDS             2168..2635
                     /locus_tag="ACGE36_001352"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383753.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2720..3604
                     /locus_tag="ACGE36_001353"
     CDS             2720..3604
                     /locus_tag="ACGE36_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8383754.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3718..3954
                     /locus_tag="ACGE36_001354"
     CDS             3718..3954
                     /locus_tag="ACGE36_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFF8383755.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4042..4122
                     /locus_tag="ACGE36_001355"
     tRNA            4042..4122
                     /locus_tag="ACGE36_001355"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4076..4078,aa:Tyr,seq:gta)
     gene            4129..4200
                     /locus_tag="ACGE36_001356"
     tRNA            4129..4200
                     /locus_tag="ACGE36_001356"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4161..4163,aa:Gln,seq:ttg)
     gene            4325..4495
                     /locus_tag="ACGE36_001357"
     CDS             4325..4495
                     /locus_tag="ACGE36_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383756.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            4715..5422
                     /locus_tag="ACGE36_001358"
     CDS             4715..5422
                     /locus_tag="ACGE36_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8383757.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5481..6125)
                     /locus_tag="ACGE36_001359"
     CDS             complement(5481..6125)
                     /locus_tag="ACGE36_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFF8383758.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6251..6538
                     /locus_tag="ACGE36_001360"
     CDS             6251..6538
                     /locus_tag="ACGE36_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383759.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6646..6966
                     /locus_tag="ACGE36_001361"
     CDS             6646..6966
                     /locus_tag="ACGE36_001361"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8383760.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7047..8360
                     /locus_tag="ACGE36_001362"
     CDS             7047..8360
                     /locus_tag="ACGE36_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8383761.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8357..9076
                     /locus_tag="ACGE36_001363"
     CDS             8357..9076
                     /locus_tag="ACGE36_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFF8383762.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9204..9755
                     /gene="raiA"
                     /locus_tag="ACGE36_001364"
     CDS             9204..9755
                     /gene="raiA"
                     /locus_tag="ACGE36_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFF8383763.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            9977..12511
                     /gene="secA"
                     /locus_tag="ACGE36_001365"
     CDS             9977..12511
                     /gene="secA"
                     /locus_tag="ACGE36_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFF8383764.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12637..13738
                     /gene="prfB"
                     /locus_tag="ACGE36_001366"
     CDS             join(12637..12708,12710..13738)
                     /gene="prfB"
                     /locus_tag="ACGE36_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFF8383765.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            13843..14529
                     /gene="ftsE"
                     /locus_tag="ACGE36_001367"
     CDS             13843..14529
                     /gene="ftsE"
                     /locus_tag="ACGE36_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFF8383766.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14522..15406
                     /gene="ftsX"
                     /locus_tag="ACGE36_001368"
     CDS             14522..15406
                     /gene="ftsX"
                     /locus_tag="ACGE36_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFF8383767.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
BASE COUNT         5285 a         2654 c         3429 g         4312 t
ORIGIN      
        1 gttataatag aaaagatttt gagtaagtgt atgctcattg gaattttttg gagggaacaa
       61 catggaagag atcatcgtaa gaggtggcaa tcaattaaat ggaacagtaa gaattgaagg
      121 agctaaaaat gctgttttgc ctattttagc tgctagtttg ttagcagaag aaggtattac
      181 gacattagac aacgttccga ttctttctga tgtcttcact atgaatcaag tgattcgtca
      241 cttaaacgta gatgtagatt ttgatgaaca aaagaatcaa gtaacaatcg acgcttcaag
      301 acaattagaa atcgaagcgc cttatgagta tgttagtcaa atgcgtgctt caatcgttgt
      361 gatgggtccg ctacttgcca gaaatggaca tgcaaaagta gctatgcctg gtggatgtgc
      421 gattggtaaa cgaccaatcg atttgcattt aaaaggattt caagctttag gagcaaaaat
      481 tattcaaaaa aacggctata tcgaagcaat tgcggatgaa ttgatcggta acacgattta
      541 tttagatttc cctagtgtag gtgctactca aaatatcatg atggctgccg taaaagcaaa
      601 aggaacaacc atcattgaaa acgtagctcg cgaaccagaa atcgttgatt tagcaaacat
      661 tttgaataaa atgggcgctc aagtctatgg agctggtact gaaacaatgc gtattgaagg
      721 cgtggatcat ttacatgcag tcaaccactc aatcgtacaa gatcgtatcg aagcaggtac
      781 ctttatggtt gctgctgcaa tgacacaagg caatgtgctg attgcagatg ctatctctga
      841 acataaccgc ccattgattt ctaaattgat cgaaatgggt gcagaaatca ttgaagaaga
      901 aggcggagtc cgtgtcatcg gaccaaaaca tatcttacct acagatgtaa aaacgatgcc
      961 tcatcctggt ttccctacag atatgcaagc tcaaatgaca gctatccaat tagttgcaga
     1021 aggtacaagt gtagtgactg aaacagtgtt tgaaaatcgt ttccaacatt tagaagagat
     1081 gcgccgtatg aacgctcatg tcaaaatcga cggaaatgta gcaatcatgg atggaaacca
     1141 tgaattgcaa ggtgcagaag tatatgcaac agacttgcgg gcagcagcag cattagtttt
     1201 agctggcttg aaagcaaatg gaatcacacg tgtgcgtaac ttgaattatc tagaccgtgg
     1261 atattataat ttccatatca aactacagca attaggtgca gacgttgaac gtgtagacat
     1321 ggatcaaact tctgctgaaa aaacagcaca aacgattgct taaggaagtg gctgaatgag
     1381 taaccaacga tatatcttat taactttgat aaagatttta atggtgatct tattgcttat
     1441 ccttcttttt gtggccggaa cgatgatcgg atatggtgtc atcggtggcg gcaatccatt
     1501 taaagtattt cagccaagtt tgtggattca tattcgagac ttttttcatt gataaataag
     1561 aatcacagtg gttctagtcg atatgaagtg agccatgaag cagaacatga taaaagaaaa
     1621 cagaagggaa cgttgacgtt agtcgcgatc ccttttttta gtaaaagaaa agaaggaatc
     1681 taattgatga agaagatatt gatttattta gttagaggat atcagcgctt tatttcgcct
     1741 gcatttccac ctagctgccg ttattaccca acctgttcca attatatgat aaaagcaatt
     1801 caaacccatg gcgcgctaaa agggacggta atggggactg cacggatctt acgctgtcat
     1861 ccttttgtca aaggcggtat cgattatgta ccattgacat tccgattaac gcgaaataag
     1921 aaagataaaa aagaaccaga ttataaaaag gttgtgaaaa atacgggtag cactgagcga
     1981 taaagaaaca atttggaaaa tagcttctta tattttgcaa aattgtgagt tattgccgaa
     2041 gtgttggatt ttgaacaccg tcaaaaaaaa ggttgtgaaa aatacggcta gcactgagcg
     2101 ataaagaaaa agaggaaatt tattgtcaaa gtaacaactc cgcaactact tatatggggg
     2161 acaaaaaatg atcgaagaac taaacaagaa agatttacca tggtccttgt tattagatgc
     2221 agatcctgat aaagaaaaag ttcaagtata tgtttccaga gggagtggct ggatatggaa
     2281 agaaaaagaa aaaacaatag gtgtactgat ctatgtggca agggaaaaag aatacgaaat
     2341 cgtcaatgta gcagtaaccc caacgtatca aggaaagggg attggcggaa aattacttga
     2401 gacagctttt cagaagcttt cacaactcgc ttccagtcaa actcagatca tcatacgaac
     2461 cggcagtact agcagtgcgg cgttacatct ctatcaaaaa atgggctatg tggagattgg
     2521 tcgagtcaaa gactatttta tccatcatta ctctgagccc atttttgaaa atggagatca
     2581 attaagggat caagtgattt tagcaattca cttacctaaa aatagcttta actaataaaa
     2641 tttcaattca agaaaatgct ttcaaatgct aaatagatac attacaatag ctttagcaaa
     2701 caaaaaggag aatattttta tgggaatatt agtttttgat tttggtggat cggctgtcaa
     2761 atatggttgt tgggatggca aagagattaa aggtaaggga caatttgcaa cgcctgaatc
     2821 ttgggaagaa atgaaagcgc aattatttca agtttataaa aaaatactcg tttcttttga
     2881 agtcgaagga attggaatca gctctcctgg agtagtgaat aatgagaaac aggtgattga
     2941 gggaatcagt gcgattcctt atattcatgg atttaatatt ttccgagatt tggaaactct
     3001 gtttcaactt ccagtaacga ttgaaaatga cgccaactgt gccggaatgg ctgaatttta
     3061 tgaaggtgct gcccgcgatt atcaagatgt tgcttttgtt gtggcaggaa ctgggattgg
     3121 cggggcgttg ttcacgaaag gacaaatcaa taaaggggca catctttacg gtggagaatt
     3181 tgggttgatg ttcttaaatg gcgataagac attcagtaaa cttggtacag cggtacagat
     3241 ggcttggcgt tattgtgaac gcaaaggtct cgataaaaat gcttataccg gaaaagatgt
     3301 ctttgaatta gcagaaactg gagatgcaat agcaaaagaa gaagttgaga gtttttatac
     3361 gtatcttaca aagggattat tcagtattca attttcgttt gatccagaag tgatcgtatt
     3421 aggcggcggg gtttctgcaa aagaagggct cattgatgag atcaaaagcc gaatgaagaa
     3481 gctgacagaa caattcgatc tgcatgattt cgaaccgcag attttattat gtgaatacag
     3541 aaacgatgct aatcttgtag gtgcagcagc aaactatctc gctgtacgtc aaggggagaa
     3601 ttaagagctt tagagaaggt gcagcttgca taaaagaagt gaatgaaatg aataggagat
     3661 ttttacctaa ggaatgtttt atttgctgca gtatgataaa atatctgaga ggtgaaaatg
     3721 atgtcaaaaa aaaataaaga tattgaagtg cgtatagaag aagtgaaaaa agatatcaaa
     3781 ggtaagaatt atgaagtcac tcagttattt atagggaaaa aaatgattgg tgagatctta
     3841 gcctatggac caaaagaata tgaaattttc tttggtgaag aagattttgg aaaagaaaaa
     3901 agtcttgaaa atgcgataga aacagtgatt cgacactgga atctgcacga ataaaaaaat
     3961 atcaaaaaaa ctttcaataa agggttgcaa aaatctggaa aattaggtat tataactaag
     4021 tcaggttgat acaaaccttt cggaggagta gcgaagtggc taaacgcgac ggactgtaaa
     4081 tccgttcctt agggttcagt ggttcgaatc cactctcctc catttcattg gggtatagcc
     4141 aagcggtaag gcaagggact ttgactccct catgcgttgg ttcgaatcca gctaccccag
     4201 tataccacaa tcgtaagctc tcgttagttc gagagctctt ttttttgctt tgctaccttg
     4261 ttccttgaag attctgctga tttaagatag agttaagtta actacaagaa ttggagggat
     4321 ccacatgaaa tggaaagaaa aagtagacga agcagctgaa aagttatatg atttggtcaa
     4381 atcagaaaaa tacaatattg aagtgaatat tccgaaaaaa ggcggaaaag cggtccgggt
     4441 aaaatcaaaa cgcccaacga atcatacaaa aaaatggatg gctaaaaacc gctaataata
     4501 acaaaagatc cataagtcgg cattttcatc gattttaggg tcttttgtta ttttctaggt
     4561 tggacatatc taaaaaattg tttctgacaa taggagtagg aagaacgatg tgtgtctaaa
     4621 tcctctgttg atggatagaa acctttatct ttttcagttg tgaatcaggt atataccagt
     4681 tataatagaa gaaacgaaag gacgggtgta cgttatgtcg aatcaattac ctgtatatat
     4741 acagattcat gaccaattaa aaaatgaaat cgaacaaggt atatggaaag tcggagaccg
     4801 tcttccttca gaaagagaat tagcagtgaa attcaatgtc agccgtatga cgttgagaca
     4861 agcaattcaa aacttggcag atgaaggaat cttagaaaga aagatcggtt cgggtacata
     4921 tgttgcacga gaaaaagtac aagaaaaaat gtctggtgca acaagtttta cagaaattat
     4981 ggaatcacaa aatcgagtac cgtctagtcg gacaatctct tatttcttga cttcgccaag
     5041 ctcaagtgaa atggaaaaac taaacttgag taaagatgac acgatcttgc gaatggaacg
     5101 tattcgtttc gcagatgatg tccctatttg ttttgaagtt gccagtgtgc cacaaaagct
     5161 gatcgatggc tacagcaaag cagagattac tgaatcgttg tatcgcacgc tagaagaaaa
     5221 aggcgggaaa aagatcggtc cagcgaatca aacgattact gctgtgttgg cttctgagaa
     5281 aattgctgac tatttagagg taaaacgagg agatgcagtt cttcgtttgc gccagatttc
     5341 ttattttgaa gacggtacgc cgtttgagta tgtgcgtacg caatacgtag gaaatcgttt
     5401 tgagttttat ttggaaaaat aaaaaacagg tgatccctga ttccgtatta gaaataaaag
     5461 agaatcagaa tcacctgttt ttatatatga tcagttacca gttgctcgtg ataagaaaca
     5521 ggaccctctt caaattggac ttgtccgttc agcagctcaa tgatggattc tttaaagtgg
     5581 tcagcacttt tttcatctac agcacagatg acgctaactt gatcagtata ttggatttct
     5641 tttataaaaa aatgttggag ttcaagataa ttctgtaatt ttcctagttg cggatacgtg
     5701 atcatcacag agatctcctg ttgcaaggta ccttctacga ttccaatgtc agacaatgct
     5761 tgagagaccg catgagtgta tgctcgtatt aatccgcctg tgccgagttt cgttcctcca
     5821 aaatatcgag tgactactgc agctacattg atcagttgat tttttttgag gacttccaac
     5881 atcggtatgc ccgccgttcc acttggttct ccatcatcgc tgcttcgctg gatttcattt
     5941 ttttctccaa tgatgaaggc gctgcagtta tggttcgctt tccaatgttc tttttttatt
     6001 tcttgaatga aactttttgc ttcgtcctct gattctattc gtctaagaga acagataaag
     6061 cgtgattttt tgatctcgat ctcactttgt ccattttctt ttattgtacg atagcgctcg
     6121 atcatcttgt cacctctttt ttcattttct ttattcagta taaataaagt tctaggttgt
     6181 ttcaacactg taacggttgc gcaattgctg tagtatgcga taataaagac aaaggcaggt
     6241 ggatctgggg atgcaggaac gaatgaaaaa gtacgatgca attacgcatt atttgaagaa
     6301 caatggcggc tctcaagtaa cgttgacctt tacccaattt gacgaactgc tgtttcccag
     6361 taacggactt cccaaaacag caagggaaag tacagattgg tgggcaaatg attataaaca
     6421 ccctgaaaaa ggtgcctatg gctggataaa tgcaggctat gaagtagttg tcatcaattt
     6481 ggacaaagaa tatgtcgtgt tcaataaatt agtgaaatct agttggctat ttgattaaat
     6541 aaaccgcgtt tagaaaacgt tgacaaattt ctttcttttt atataataga gtaacagacg
     6601 cgaagaaaac gtttgcgtag tttgttttaa aaaggagaga aaagtatggc taaaaaaaca
     6661 atcatgcttg tatgttcggc tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa
     6721 gctgctgaag caaaaggaat cgactctgat attttcgctg tgtccgcttc tgacgcagat
     6781 aacaatcttg caaataaaga cgtggatgtg ttgttattag gcccacaagt tcgctttatg
     6841 aaagctgatt ttgagaaacg tctggaacca aaaggcattc ctttagatgt catcaatatg
     6901 gctgattatg gtatgatgaa tggcgaaaaa gtgttagatc aagcaatcac gctcataggt
     6961 gaataaatag aacagtataa ctatcgaaca agcgatccaa aaaatagatc gcttgttttt
     7021 tcgtatattt ttttagggcg aataatatga atcatttttg gggtagaagg cttctgacga
     7081 gagatattaa agcaatagac tgtgaggagg taaatttacc aaattatgag aagatcgcag
     7141 cagtggaacg tttgagcaac tggattcttt gtcatcgctg tggaatgaga acgtccgtca
     7201 ttgatggaca gcttgcagat tacggttatt tttgtacaca ttgtctatat ttagggcgat
     7261 gcgacactca acaagattta tatttgtttg atcagcctga agcaaaacct cgagaagtcg
     7321 tctttttttg gacaggacag cttactgaaa aacaaacgga aatctcaaag agaatacttt
     7381 gccattcaga aaaaagacat catcttattt gggctgtaac aggtgcaggg aaaacagaga
     7441 tgctttatcc tattttagtg gaaactttaa aagctggagg aagagtagcg atttgtacac
     7501 cgcgagtcga tgtttgtaat gaattgtttt tacgctatcg ttatgttttt ccagaagaaa
     7561 aaatcacgtt gctccatggg aatacagcaa cggcttatac ctttacttct tttgttatct
     7621 gtacgattca tcagctatac cgtttttatc ggtcctttga tttaatagtg atcgatgaga
     7681 tcgatgcgtt tccttataac ggggatgcag gattagctca tgctgtgcaa acagcgatta
     7741 aaccagacgg acgattcatc tacttaagtg ccacacctga taaaaagtta ctaaaggaga
     7801 tcaagcagac gtttgacatc cacaaacttg ctttacgctt tcatcggcgt ttgcttcctg
     7861 aacccgtctt ggttttcatg aataattggc gacaaaaatg caagaacaag aagaagataa
     7921 aatctttggt gcggatcatt catcaattaa ttaaagaaaa ccatatcctt ttattttgtc
     7981 caagtatcac gatgatgcat cgtttgaaag ccacacttca agaggctctt ccgagatatg
     8041 agatcacaga tgtttcttca agagacaatc agcgggcgga aaaagtccag aaaatgcgag
     8101 aagcaaaata tgacatactg ttaaccacga tgatccttga acgtggagtc acatttgaaa
     8161 gaatatccgt tatcgtttta ggagcggatc atccggtttt tactaaatct tctttggtac
     8221 aaatcgcagg aagagccgat cgtaaagggc cttatacgaa cagtcaagta tattttttct
     8281 atgaagaaaa aactgcagct attcgaaaag cttgtcagga aatcaaagaa atgaatgaag
     8341 aagggaagaa gatactgtga ggtgcagttg ctgcagtaaa gatatcgtcc aaaatttaac
     8401 attcagcgag attattggtg tttcgaaata cagattacct cagacttgtt cagtttgtcg
     8461 cgcacaattt catttattgg aaacaagagg ctgttctggc tgtcaaaaac caatccattt
     8521 caatctagag agtacgaaaa agaaagtaaa ttctatcccc gtttactgca gtgactgtca
     8581 agaatggaag aagaaatatc cgacctatga ttttcagcac cgtgcctttt ttgtgtacga
     8641 tgaaaatatg aaggcctggc tcaatcaata caagtttatg ggagatatcc gactagcagg
     8701 cacctttgca tataaatgga cgcagataaa aaaagaatac ccgacataca tctattgtcc
     8761 aataccttta tctgaaaaaa ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt
     8821 gaaagcagca agaattcctt ttcagatgct actcaaaaga gagaagcatc tgctggctca
     8881 agcaaaaaac acgcgaaagg aaagattgga gatgcctcaa ccttttgaac ttgcagtcac
     8941 cttttctaag atacaaaata aaaaaatatt gattattgat gatgtctata caacaggaca
     9001 gactcttttc cacgcagcag attgtttgca gatcgcacag ccgaaaatca tccgaactat
     9061 ttctttggcg agatgaatta gatgtggaaa tttgatagcg atttaatttg caaataacct
     9121 agagttcgat ataatagaat taagaagagg gaaagagtgg tccttctcct tcttgattgt
     9181 ggtagacgaa aggggtaatt tttatgttta gatataatgt acgcggcgaa aatatcgaag
     9241 taactgaagc gattcgcgac tacgttgaga aaaaagtagg caaactagaa cgctatttca
     9301 gtgacgcacc cgatgcaacg gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa
     9361 aagtggaagt aacgatccca ttaccttatc ttgtattacg tgccgaagaa acttctccag
     9421 atttatacgc aagtatcgat ttggttgttg acaaattgga acgccaaatc cgcaaattca
     9481 aaacaaaaat taatcgtaaa tcccgtgaaa cgggcatgaa tactgcaaat gctgctgctt
     9541 tattcaatga agaggacgaa aacgaaaatg attctgaatt agatatcgta cgtacgaaac
     9601 gcctttcatt gaaaccaatg gatagtgaag aagcagtctt gcaaatgaac atgctgggac
     9661 ataacttctt tatttttgaa gatgcagaaa caaatggtac aagtatcgtt taccgccgta
     9721 aagatggaaa atacggactg attgaaacag actgatcagc cttacttaaa taacaacgaa
     9781 aacttgcagc agctactgac agctgccgca agtttttttg agtttcctcc tcttttagat
     9841 gaaatatgaa taaattatga agttcttact ttaatacgct taagatattt caattatcta
     9901 taactaatga taaaatgaaa agtaaagtct acatagtctg gataatacac agaaggaaaa
     9961 ggagattaca caacgcatgg ccaatttgat tcgttcactt atcgaaaacg ataagaaaga
    10021 actaaaacgt ttagatagca ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc
    10081 attaactgat gaacaattac aggcaaaaac tgctgaattc aagggcagat accaaaaagg
    10141 agaaacatta gatcaattac ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg
    10201 tgtattaggt ttatatccat accacgtaca attgatgggt ggaatcgtgc ttcacgatgg
    10261 aaatatccct gaaatgcgta ctggtgaagg taaaaccttg accgcaacga tgccagtata
    10321 tctaaatgct ctgactggcg aaggggtaca cgtagttacc gtcaatgaat atttagcaac
    10381 ccgtgactcg acggaaatgg gcgaacttta taatttctta ggattaacag tggggttgaa
    10441 tatcaactca aaatcttcgg atgaaaaacg cgaggcttat aattgtgata tcacttatag
    10501 tacgaataat gaattaggat ttgactactt gagagacaat atggtcgttt accgccatca
    10561 aatggtacaa cgtccgctga attatgccat tgtcgatgaa gttgactcaa tcttgattga
    10621 tgaagcgaga acgccgctga tcatttcagg gcaagcagaa aaatccacag ctttatatac
    10681 aagagcagac aacttcgtga aacgtttgaa agaagaagaa gattataaaa tcgatgtaca
    10741 atcaaaaaca atcagtttaa ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt
    10801 ggataactta tacgacattg aaaatactgc attgacccat cacttggatc aagcattgag
    10861 agccaactat atcatgcttt tagatatcga ttatgttgtc caagacggaa aagtactgat
    10921 tgttgaccag ttcactggtc ggatcatgga tggacgccgt tattcagatg gacttcacca
    10981 agcaatcgaa gctaaagaag gcgtagagat cgaagacgaa acgaaaacca tggcgacgat
    11041 cactttccag aactatttcc gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa
    11101 aacagaagaa gaagaattca gagaaattta taacatccaa gtataccaga ttccgacaaa
    11161 tcgtccggtt atccgtgatg atcgtccaga tttgctttac ccaacattac aaagcaagtt
    11221 ccacgctgtc gtgcaagata tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg
    11281 aactgttgcg gttgaaacat ctgagttatt atcagatatg ctgaatcaag aacgtattcc
    11341 tcatgaagtg ctgaatgcga aaaatcactt taaagaagca gagatcatca tgaatgctgg
    11401 tcaaaaaggt gctgtgacga ttgctaccaa catggctggt cggggaacag atatcaagtt
    11461 aggacttggg gtaagagaac ttggtggtct agcagttatc gggacagaac gacatgaatc
    11521 tcgtcgtatc gataaccagc ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc
    11581 acagttctac ttgtcattgg aagatgattt gatgaaacga ttcggttcag aacgaatcaa
    11641 atctttctta gatcggatga agatcggtga ggaagatgct gttatccaaa gcaagatgct
    11701 gacgaaacaa gtagaatctg cacaaaaacg tgtagaagga aataactatg acacacgtaa
    11761 aaacgtctta caatacgatg atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg
    11821 acaagaagta atcatggaag aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg
    11881 gacaatcagt cgtgtagtag atgcgcatac acaattagag aaagaaaatt ggaatttgga
    11941 aagtatcatc gattttgccg gcagtactct tgtccatgaa gatagtatta ctcttgcaga
    12001 tatcgaagga aaaacagctc aggagatcaa agagtatcta aacgaacgag cacaagaagt
    12061 atttaatacc aaagcggctc aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt
    12121 cgtgatcttg cgtgttgtgg actctaaatg gacagaccat attgatgcaa tggatcaatt
    12181 acgtcagtca atcggcttac gtgcttatgg acagaacaat ccattagtcg aatatcaaac
    12241 agaaggctat aagatgtttg aagatatggt aggcgccatc gagtatgaag tcactcgaat
    12301 ctttatgaaa gctgagatcc gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc
    12361 ttctcactca gaagacggag atgccaaaga aaatagtaat actagcgcga aaaaacagcc
    12421 aatccatgtg aacaaggtcg gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata
    12481 taagaactgt catggaaaaa atgcaaacta atttctgaca gcaggattgg tgagacaaat
    12541 tcttttttta agaattcgtc tcaccttctt tctgttattt gctgattctg ttagactaga
    12601 tataatgaaa aaagtatagt gaaagaagga aaaaacatgg aaatatccga aattcgaaat
    12661 ctcctgacag aaatgcagga aaaggtcaca agcttcagga ggtctctttg acttagacca
    12721 gctggaagaa gacattgcgg aagcagaagc aaaaatggct gaacctggtt tttgggatga
    12781 tagcgaagct gctcaaaaag tgatcagtga aaacaatgca aataaagaaa cttatgatca
    12841 attccattct cttgctgaag aactggaaga aatcgaattg atggtggaga tgttgcagga
    12901 agaacctgat gcagccatgc aagaagaagc agaaacacgt atcaatgcgc tagatgaaaa
    12961 aatgaagaca tatgaattat ctatgttgtt agatggtcct tatgatcgaa acaacgccat
    13021 cgttgaattg catccagggg ctggcggaac ggagtcccaa gattggggca gtatgctttt
    13081 acgtatgtat acacgctggg cagagcagca tgggtatcaa gtagagacac tagattatca
    13141 agcaggtgat gaagcaggaa tcaagagtgt gaccctactg atcaaaggac acaacgcatt
    13201 tggttatctg aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc cttttgactc
    13261 agcgaaacga cgtcatactt ctttttgttc ggtagatgtg atgccagagc ttgatgaaac
    13321 gatcgatata gaaatcaatc cagatgattt gaaaatcgat acgtaccgag caagtggtgc
    13381 aggtggtcag cacatcaata aaacagaatc tgctgtacgg atcactcact tgcctacagg
    13441 aacagtagta gctagtcagg cacaacgttc acaattgaaa aaccgtgagc aagcgatggg
    13501 gatgttaaaa gctaagcttt atcaattaga agtggaaaag aaagaacaag aagcagctgc
    13561 acttcgagga gaacaaaaag aaatcggctg gggttcacag atccgttctt atgttttcca
    13621 tccatattct atggtaaaag accatcggac aaacgtagag acaggaaatg tccaagcagt
    13681 tatggacggt gaccttgatt tatttattga cggctattta aaaatgcatc tacaataaga
    13741 acccattgtt aaattgaaat aaaattgtaa gattttgcaa gcatgttgaa acattgccat
    13801 gctataatag ctaaagactg aaaaagatat ggagagaacg ccatgattga aatgaaggat
    13861 gtaatgaaga agtactcaaa tggtacgacc gctatccgca atctctcaat tgtcatcgaa
    13921 caaggagagt ttgtctatgt ggttggtccg tctggagccg gtaaatctac attcatcaaa
    13981 ttgatgtatc gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg tcatgatcta
    14041 atagcaatca aaaaccgcga agttccttat ctacgcagag aaatcggcgt ggtgttccaa
    14101 gactataaat tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt
    14161 atcggcagaa aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt
    14221 ttgaaacaca aagttcgagt ctttcctagc gaattatccg gtggggaaca gcaacgtgtc
    14281 tctattgctc gggcaatagt gaatacgcct aaagtactta tcgcggatga accgacagga
    14341 aatctggatc cagaaaactc atgggaaatc atgaaattat tagatcggat caatgcccaa
    14401 ggaactactg tcgtgatggc tacacataac agtacgatcg taaatacgat tcggcaccga
    14461 gtaattgcta tcgaaaacgg ccgcattatt cgagatcaag cggaagggga atatggatac
    14521 gatgattaga acctttttcc gccatttatt agaaagtatc aagagtttga aacgtaacgg
    14581 ctggatgaca gttgcttcag tcagtgcggt aacgatcaca ttgactttag tcggtgtatt
    14641 catggcagtt atcatgaacg caactaaatt agctcaagat atagaaggaa acgttgatgt
    14701 ctctgtattc gtagatatcg gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt
    14761 gaatgactta gatcatgtta aaaaagttga attttcaagc aaagaacagg aattgaaaaa
    14821 aatccaaaac gaaatgggtg attcctggaa attgtttgaa ggtgacaata acccgttata
    14881 tgatgtgtat gtagtaagtg ctactgatcc ttcttataca aaacaagtag cagaagaagc
    14941 agcagatttg aagaatgttt ctcgttctga ttacggtgga gcttcctcag atcggatttt
    15001 ccaaatcgct ggtgcagttc ggacatgggg attagttgca gcggcattgc tgctgttcgt
    15061 agcgatgttt ctcatttcga acacgattcg gatcacgatt ttgtctagac agcgtgaaat
    15121 tcaaatcatg cgtttagtcg gtgcaaaaaa tggatatatt cgctggccgt ttttccttga
    15181 aggtggctgg atcggtctga tcggtgcgat tcttccgatt ttgatcatca catttggtta
    15241 tgcatggttt ttccaattga tcaatccatc attattacgt tcccattatt cattgattca
    15301 tccacaaaat atcgtttgga aaatcaatct tttaatggtc ggcattggtg tgatcattgg
    15361 ttcacttggc tcgatcattt ctatgagacg tttcttgaaa ttctaatacc acaatataaa
    15421 taaaatgaga aagttggaca gtttcgttcc agctttcttt ttttattggc taggagaata
    15481 gaaggaagcg gcttttttcc ctttacttta aaaaacaact tgggtattct taagttggta
    15541 gaagaagagg tgaagccgtg aaaattatcg ttgacaattt ttttacgatc cttttgatta
    15601 tgaatatttt gctttccttt atcatcgttt ttcgagaaag aaaggaaact gctcaaacat
    15661 gggcatgggt gctcgttttg
//
LOCUS       DAXWLH010000057        15548 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXWLH010000057 DAXWLH010000000
VERSION     DAXWLH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.057"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(73..792)
                     /locus_tag="ACGE36_001369"
     CDS             complement(73..792)
                     /locus_tag="ACGE36_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8383768.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(923..2074)
                     /locus_tag="ACGE36_001370"
     CDS             complement(923..2074)
                     /locus_tag="ACGE36_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFF8383769.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2291..3031)
                     /locus_tag="ACGE36_001371"
     CDS             complement(2291..3031)
                     /locus_tag="ACGE36_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8383770.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3035..3391)
                     /gene="rsfS"
                     /locus_tag="ACGE36_001372"
     CDS             complement(3035..3391)
                     /gene="rsfS"
                     /locus_tag="ACGE36_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFF8383771.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(3415..4008)
                     /gene="yqeK"
                     /locus_tag="ACGE36_001373"
     CDS             complement(3415..4008)
                     /gene="yqeK"
                     /locus_tag="ACGE36_001373"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFF8383772.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3992..4642)
                     /locus_tag="ACGE36_001374"
     CDS             complement(3992..4642)
                     /locus_tag="ACGE36_001374"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFF8383773.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4652..4963)
                     /gene="yhbY"
                     /locus_tag="ACGE36_001375"
     CDS             complement(4652..4963)
                     /gene="yhbY"
                     /locus_tag="ACGE36_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFF8383774.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4979..6088)
                     /gene="yqeH"
                     /locus_tag="ACGE36_001376"
     CDS             complement(4979..6088)
                     /gene="yqeH"
                     /locus_tag="ACGE36_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFF8383775.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6085..6612)
                     /locus_tag="ACGE36_001377"
     CDS             complement(6085..6612)
                     /locus_tag="ACGE36_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFF8383776.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6839..7624)
                     /locus_tag="ACGE36_001378"
     CDS             complement(6839..7624)
                     /locus_tag="ACGE36_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFF8383777.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(7617..8486)
                     /gene="accD"
                     /locus_tag="ACGE36_001379"
     CDS             complement(7617..8486)
                     /gene="accD"
                     /locus_tag="ACGE36_001379"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFF8383778.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8487..9866)
                     /locus_tag="ACGE36_001380"
     CDS             complement(8487..9866)
                     /locus_tag="ACGE36_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFF8383779.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9874..10293)
                     /gene="fabZ"
                     /locus_tag="ACGE36_001381"
     CDS             complement(9874..10293)
                     /gene="fabZ"
                     /locus_tag="ACGE36_001381"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8383780.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10318..10794)
                     /gene="accB"
                     /locus_tag="ACGE36_001382"
     CDS             complement(10318..10794)
                     /gene="accB"
                     /locus_tag="ACGE36_001382"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFF8383781.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10797..12032)
                     /gene="fabF"
                     /locus_tag="ACGE36_001383"
     CDS             complement(10797..12032)
                     /gene="fabF"
                     /locus_tag="ACGE36_001383"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFF8383782.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12051..12788)
                     /gene="fabG"
                     /locus_tag="ACGE36_001384"
     CDS             complement(12051..12788)
                     /gene="fabG"
                     /locus_tag="ACGE36_001384"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFF8383783.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12800..13717)
                     /gene="fabD"
                     /locus_tag="ACGE36_001385"
     CDS             complement(12800..13717)
                     /gene="fabD"
                     /locus_tag="ACGE36_001385"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8383784.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13824..14048)
                     /locus_tag="ACGE36_001386"
     CDS             complement(13824..14048)
                     /locus_tag="ACGE36_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8383785.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14101..15063)
                     /locus_tag="ACGE36_001387"
     CDS             complement(14101..15063)
                     /locus_tag="ACGE36_001387"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFF8383786.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15073..15513)
                     /locus_tag="ACGE36_001388"
     CDS             complement(15073..15513)
                     /locus_tag="ACGE36_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8383787.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4070 a         3552 c         2677 g         5249 t
ORIGIN      
        1 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
       61 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
      121 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
      181 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
      241 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
      301 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
      361 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
      421 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
      481 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
      541 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
      601 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
      661 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
      721 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
      781 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
      841 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
      901 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
      961 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
     1021 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
     1081 ttccaacatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
     1141 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
     1201 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
     1261 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
     1321 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaat
     1381 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
     1441 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
     1501 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
     1561 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
     1621 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
     1681 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
     1741 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
     1801 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
     1861 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
     1921 tatcgatgat agccggttcg cctcgttgca aaaaattccc gctcattaca gcaatgacga
     1981 cctctgctcc tgttgttttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
     2041 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
     2101 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
     2161 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
     2221 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
     2281 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
     2341 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
     2401 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
     2461 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
     2521 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
     2581 attctggaaa aatttcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
     2641 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
     2701 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
     2761 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
     2821 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
     2881 aggcaactgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
     2941 gtaaatgacg tttcgaaaaa gctagccatt gctgataaag tgtttcgtcc atcacttcat
     3001 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
     3061 ctagcggagc atctgaccat agtttttcca gattatagaa acttcgttct ggtgcatgga
     3121 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
     3181 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
     3241 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
     3301 catttcgaat gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaaccg
     3361 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
     3421 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
     3481 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
     3541 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
     3601 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
     3661 tgcatgaaga atttctacat cggtgattcc taattcccgc tctacaaacg aagcgccaac
     3721 aagaccatgc cagattgcat tattataatt caataactct ggatcaaaac cgtctcttac
     3781 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
     3841 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
     3901 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
     3961 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
     4021 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
     4081 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
     4141 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
     4201 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
     4261 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
     4321 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccacag
     4381 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
     4441 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
     4501 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
     4561 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
     4621 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
     4681 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
     4741 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
     4801 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
     4861 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
     4921 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
     4981 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
     5041 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
     5101 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
     5161 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
     5221 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
     5281 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
     5341 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
     5401 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
     5461 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
     5521 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
     5581 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
     5641 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
     5701 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
     5761 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
     5821 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttctgcccta
     5881 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg atctcattat
     5941 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
     6001 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
     6061 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
     6121 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
     6181 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
     6241 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
     6301 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
     6361 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
     6421 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
     6481 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
     6541 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
     6601 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
     6661 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
     6721 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
     6781 acaaaaaaca actaagcgag caagaccatt ctttactctt tgcttagttg ttaaacacct
     6841 aatattggcg gaacctcttg tatctttttt ccagtaattc atgctggtcg aatcgaacaa
     6901 gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca
     6961 actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt
     7021 ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca
     7081 aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga
     7141 cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg
     7201 cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt
     7261 cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc aatgtaatca
     7321 ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag
     7381 gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac
     7441 cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat
     7501 ctgcaaacaa acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat
     7561 ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat
     7621 ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgcttc
     7681 aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt
     7741 tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa
     7801 ccgatcaagc tttggggttc tgctagaatg atatcgcctt ccattgcaaa actggcagtt
     7861 actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc
     7921 cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc
     7981 cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg
     8041 aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga
     8101 tccatcactc caataatgaa gggaatccct tgaatggttg ctttaccagt gaggactgct
     8161 tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct
     8221 agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc
     8281 aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt
     8341 tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg
     8401 ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata
     8461 tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa
     8521 ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcaggg
     8581 tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca
     8641 cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt
     8701 tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct
     8761 acccgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga
     8821 gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat
     8881 actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt
     8941 actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc
     9001 atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt
     9061 tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt
     9121 tgtgagatca ctacagaagg agattcttcc agtacttttt gattattacg ttgcaaagaa
     9181 caatctcgtt ctcctaaatg aatgacattg ccaaaatgat cacctaaaat ctgaacttct
     9241 atatggcgag caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct
     9301 gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc
     9361 cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata accaattctg
     9421 tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact
     9481 gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc
     9541 gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg
     9601 ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca
     9661 gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag
     9721 attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct
     9781 acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg
     9841 ttagctatca atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc
     9901 ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt
     9961 cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa
    10021 ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag
    10081 taatgcaact gctcctgctt gagccatcgc ttcaacaatt aatacccctg gcataacagg
    10141 ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac
    10201 gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg
    10261 tggaatgatt tcttttattt cttgtatgtt cattgatttc cctctttcct gctttgctta
    10321 tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag
    10381 gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc
    10441 aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt
    10501 gtcgggcgcc ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc
    10561 aaatactgcc gattcttttt ctttcattgt ttctggagca gttgttgctt gttcaggtat
    10621 gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg
    10681 acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt
    10741 tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat
    10801 tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc
    10861 attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca
    10921 ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa
    10981 gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg
    11041 ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct
    11101 gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc
    11161 tgtacaccag cttcttccat cgcaagcttc attgctttag acgcaccaga accatctgga
    11221 gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaatgat ttctccatag
    11281 attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac acctgctcct
    11341 tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca
    11401 gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt
    11461 gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct
    11521 tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt
    11581 gcacctacgc gcaaagcaat atttccagct gccatgttag caatcgccat tggtacgaac
    11641 attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt
    11701 ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct
    11761 ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac
    11821 atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact
    11881 tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca
    11941 ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg
    12001 actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc
    12061 atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag
    12121 aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt
    12181 tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct
    12241 ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt
    12301 aatcctacta cgccagcttt actagctgca taatttgcct gaccaatatt tccaatcaac
    12361 ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat
    12421 acgtgctgcg tcatattgaa ggttccaatc aaattgatat caagacattt tttgaaatct
    12481 tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca
    12541 tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca
    12601 gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt
    12661 tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct
    12721 aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt
    12781 acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct
    12841 caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc
    12901 cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat
    12961 agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca
    13021 ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac
    13081 ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc
    13141 tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt
    13201 ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag
    13261 atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat
    13321 tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata taagcgcctc
    13381 tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg
    13441 aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata
    13501 agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca
    13561 aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt
    13621 catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt
    13681 gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa
    13741 tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc
    13801 agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct
    13861 tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca
    13921 ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc
    13981 aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg
    14041 aataccataa taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt
    14101 ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac
    14161 tttctgctgt tttcccaagg tgatctttcc actagaaact gcttcatcca ataagatagg
    14221 gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg
    14281 catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag
    14341 ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc
    14401 aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga
    14461 atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc
    14521 agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc
    14581 tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag
    14641 tgtttcacca ccaatcacta gtcccttagt tgagccgcag cggatcattt tttcagccat
    14701 gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt
    14761 tgctcccaat ttcccttgta cttgtgctgc tacagatggc gtcgtgtaat cgggcgacat
    14821 tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt
    14881 ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg
    14941 aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt
    15001 attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc
    15061 cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag
    15121 caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg
    15181 aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac
    15241 gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat
    15301 agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt
    15361 tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa
    15421 ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag
    15481 ataatcattt actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat
    15541 tatcaaat
//
LOCUS       DAXWLH010000058        15508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXWLH010000058 DAXWLH010000000
VERSION     DAXWLH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.058"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..606
                     /locus_tag="ACGE36_001389"
     CDS             <1..606
                     /locus_tag="ACGE36_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383788.1"
                     /translation="KEQEEKQRQKEKEREQLEIRKREQDLAEQKMAIEKQRLEQEKEK
                     EAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQANLTPSPQRSFNGWMVGCI
                     ASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQEQARKEYEQQLEELLNRTNQ
                     STTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            606..3218
                     /locus_tag="ACGE36_001390"
     CDS             606..3218
                     /locus_tag="ACGE36_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFF8383789.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            3215..5593
                     /locus_tag="ACGE36_001391"
     CDS             3215..5593
                     /locus_tag="ACGE36_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8383790.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            5654..6196
                     /locus_tag="ACGE36_001392"
     CDS             5654..6196
                     /locus_tag="ACGE36_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383791.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            6274..6606
                     /locus_tag="ACGE36_001393"
     CDS             6274..6606
                     /locus_tag="ACGE36_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383792.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            6607..7200
                     /locus_tag="ACGE36_001394"
     CDS             6607..7200
                     /locus_tag="ACGE36_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383793.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7222..8916
                     /locus_tag="ACGE36_001395"
     CDS             7222..8916
                     /locus_tag="ACGE36_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFF8383794.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            9604..11481
                     /gene="ltrA"
                     /locus_tag="ACGE36_001396"
     CDS             9604..11481
                     /gene="ltrA"
                     /locus_tag="ACGE36_001396"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8383795.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            11612..11989
                     /locus_tag="ACGE36_001397"
                     /pseudo
     CDS             11612..11989
                     /locus_tag="ACGE36_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            11989..13194
                     /locus_tag="ACGE36_001398"
     CDS             11989..13194
                     /locus_tag="ACGE36_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8383796.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            13210..13854
                     /locus_tag="ACGE36_001399"
     CDS             13210..13854
                     /locus_tag="ACGE36_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383797.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            13865..14671
                     /locus_tag="ACGE36_001400"
     CDS             13865..14671
                     /locus_tag="ACGE36_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383798.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            14693..14923
                     /locus_tag="ACGE36_001401"
     CDS             14693..14923
                     /locus_tag="ACGE36_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383799.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         5687 a         2453 c         3215 g         4153 t
ORIGIN      
        1 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
       61 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
      121 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
      181 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
      241 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
      301 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
      361 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
      421 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
      481 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
      541 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
      601 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
      661 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
      721 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
      781 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
      841 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
      901 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
      961 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
     1021 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
     1081 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
     1141 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
     1201 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
     1261 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
     1321 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
     1381 tgagtgataa tccattggca ctcattgtga aatactggaa gcgaggagac atagacaccg
     1441 caacacagtt aaccaatact tttacgaata cgatttatta tgatgcaaat gcttcagaca
     1501 ataaatactt taacgaaaat gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg
     1561 aacaagcgga tcaaacaggg gattactcca aattaacacc aaataatctc gttgaactac
     1621 tttcagaaat tggcggtttt aattattatc cagatcctga gaatgaattc aagcagctca
     1681 atgccttaga tgagttcatg aatcagttac cccctggaaa tatagcaaaa aaacaatatg
     1741 gggcaacaaa aattggatct gataaagcga aagggaatat cttgtctacg gcaattacag
     1801 gattgaatcc atttacccta acgaaaattg cgaagatgac ttctcaatcg accattgatt
     1861 acaagtccat tggttttccg aagtatttgg atctcaaatt acatgaaagc ctattaaatc
     1921 aacgcattgt cgtggaattt tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag
     1981 ttggctatcg tggtttttgt gaaatcaact ttgattgtca tttagagcag ggagatcatg
     2041 tttgcttaaa atacaatcat caaggaaaac aatatgttgc ttggtatgag ttttctcaaa
     2101 aagtattgaa agacgaagag ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt
     2161 tgccagaact agaaaaagaa gccgttctaa cattagatga gaagaaaagt aacctattag
     2221 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg ttaacgcctg
     2281 attatgatcc atctaaccat atcattgtta gcatctttct tagtcagttg tataaagaat
     2341 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgtaggatt cattttattt
     2401 tagatgaatt tggaaatatg ccaccgatgg acaacatgga aggaatcatg acggttacgg
     2461 caggtcgtaa tatgttatgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
     2521 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
     2581 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
     2641 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
     2701 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
     2761 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
     2821 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
     2881 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
     2941 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
     3001 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
     3061 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca acggatcgtc
     3121 ttcaagaata tgttgaaaag ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac
     3181 ggctgttatt ggaagctgaa aaggcggtga aagaatgatc attggaggaa ttttctctgg
     3241 catagccgac tggtggtcag gtttattcgg agaaggggat gcagatccca aaaccgtaac
     3301 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattca gttggatttg
     3361 taatactttt ttatggggat tgatcaaagg gttatataat ctcaatactt tattagaaaa
     3421 aacaatttat caaagctttg atttaaaaga cttattgaac gccgctggtg ttaatgaact
     3481 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
     3541 aggcttgaaa ttttttatga gtaaacaccc accgaaagtc aaaaatatct tggttaattt
     3601 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
     3661 cagtaaaaac ttttatggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
     3721 tgcttttcaa attattcaaa ataatgtgat tgatgtcagt acattactag cgactgaccc
     3781 gaataaagta gaatccttac caacagataa aagaaatgct ttaacagcga aaaatttcaa
     3841 aacagcgaat atcaatggga ttattacacc tgatcaagct aagaaaatgt ctgaatcaag
     3901 tgatattagt acagtacaaa aagatcggtt aaaggcatta cagtatcgac tagagttaga
     3961 cgataatggg aaagaaatcc ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta
     4021 tacgtcaggc tatcgtcgat acattagtag tccagggatt attttggctg gggaatcttc
     4081 tttgtcggta gcctatttat ttattttgtt tacaatcgtt acttgcatta ttgagctagt
     4141 atttaaaaaa ttctatttag taattgccgc gtcaacggac tttgagacag gacaacggat
     4201 gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta cgattttaga
     4261 attacgaatc tatacattaa tgctttcagg tatcggtgat ttgcatgcgg taggaaagat
     4321 caatggattc ttgtatgttg ttgctttaat tgtgctaact attgcgctat tcaaaggctc
     4381 tcaatcggtt acgaagattt tcggtgttga tacgagtctg aaaaacggca gcaattctct
     4441 tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct
     4501 tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaga atggatttgg
     4561 ccatcgaaaa gatattaccc atgataacgg ggaagaacaa tcagacgata aagctacaac
     4621 tgtacctaat agtaagaaga cagtttccga aatgttcggg aaagcgaaaa atggtgtttc
     4681 taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga
     4741 tcttacagaa aaagccaaag acaccttaga agagaaatta gacggcacga gagctaaaga
     4801 atttgctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag aaaaagcttc
     4861 agcagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg cagcagtgaa
     4921 ggcaaataca acaagtgaaa aagaaaatca agaaggtgca gcgattcctt cagccaaaga
     4981 tcaccaagat gagttacatt ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg
     5041 acaacatggt cagttaccaa aaaccgatcc agaacaatat cgttataaat ctaacattaa
     5101 ttctgaaaac gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga
     5161 tgaggattcc atcgatctgc caaatgataa tggcatcgaa gttggaactg aaaaatcaac
     5221 gaaaattggt ttgcctaaga cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt
     5281 caaagagaat gatcaaagct ttgttcatca atcacctgca ggtactgttc aaatgaaatc
     5341 aagtcgtttg cctgaaaatg gaaatctgac aactgtttct tacaatttag ctgaaagtgg
     5401 tgaaacagag aatgcgtccg ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat
     5461 caataaagtt cataatggat acagaccagc aaacgtagca tttactgcac cgacaaaaat
     5521 aaatgtgaca agagaaacta aaaacaggaa caatgaattg aaagaacaac tattgaagga
     5581 tatcaaacca taaaaggcct atttaagggc ctttttcttt ataaaacaag tttaaaatgg
     5641 aaaggagaaa attgtggagg atattttaat agctacgttt ataactgcac tgggagcatt
     5701 tttaacgata tttgtggtga ctattaaagg atttaaagat acggatagtt tacaaaaaga
     5761 tcatcagtct attttagatg gtcaaaaaac acataccgaa agcattattg tacacctttc
     5821 agaaggattc acttatttga aagatgctat gcccacattc acagtaaaat taacggaaat
     5881 atttcattct gttgagcaaa tatcgtcaga attgaaagag caaagacaac aaaaagatcg
     5941 tcaacttggc tcacttaatt ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa
     6001 tcgattaaac gatgaaatgc aacgagttca tttggaaaat gaagaattac gcgcacaaaa
     6061 ttatgattta attcaaaaga aaagaaatct ggaacaaaaa attgttgaat taaaagaaga
     6121 aaatcgtcaa ttgaattatc aattacgacg aggaaaaaat cgttctcgtg atttaggaaa
     6181 tgaattagag ttgtaatatt tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct
     6241 aaaaatacaa aatataaggg gtatggttat attatggata aatacattca tatgaaaaat
     6301 cctccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga tctattaacg
     6361 cttggtggct gctttttttg ggcgaaaata gtcaataatc ttgtccactt accactcttt
     6421 tttgcactct tgaattatgg attatggctt gcgatagcgt tagtgattgt ttggcgaccc
     6481 tataataacc ctggtcaacg ccaaattttt gcgattatcg aatctatttt ctttacggat
     6541 aagaaccact atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
     6601 gaataaatgc gagtcaaagg acaaaattca gttgataaga aatccatttt gcagtttgga
     6661 aaaccagaag agcgctctat cttggactta tttccatata aatcctgtac agaagaggga
     6721 gtcttaatta ctagagaaga tcattttcag cgttattatc gtgtcgtttc cactgacgtg
     6781 gagggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
     6841 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
     6901 atcgtgcaaa agcggcagtt attagataaa aatcgtatgg aacagtcact aggaagaaat
     6961 ctacagcgat taagaaaata tgaaaagaaa ttagtagagg agatccagga attaaaacgg
     7021 gcagaacaag aacgtcctga cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag
     7081 gatttacaaa ctagaaatcg tcagttaatg cgatctagtg gcattttagg gttaaaacca
     7141 ctctacaaaa aagaattgat taagattctt tatcgaatga acaacatgaa tgacgaataa
     7201 aaaagatgga ggaaatgatt catgctagat gtgataaaaa cagctttttc aacgcaaagt
     7261 aatcaaaagg tgattgatcc attaaaacaa cttggtaatc gtgaaataga gattttaaaa
     7321 tcaaaaggtt atgatttaga tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt
     7381 gatgaagaat atgtgaccta tggaaatggt tattcacgat gtgtgacagt ttatcattac
     7441 acaaaaaatc ctactctttt ttggttggcc aatctcatga acaatcctca tacagtggcc
     7501 acactagatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta
     7561 gatgaattaa atgatcgatc agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg
     7621 gaagaatacc tggaactagc ggaatatgcc cgaaaattga cttctaatgg ggaaatttct
     7681 aaacaagtca aagtgagagt acatgtttac ggaaatacga tccaagaagt ggatgaacgg
     7741 gcgcaggaac tgatcgaaaa tctaaaaggg atggattatc gtgcggtgac ttatctgcat
     7801 aaaaccaaac gagattggca atcgatgttt accatgttcg atgaacaagc tgaatggttt
     7861 ggaaatatca aagggcaatc acttccatcg ttaaacattg gcggaggatt cccgttccac
     7921 caccaaacgc taaaagatcc aggaggcaca caatatgggg aaaccatgac aggcggccca
     7981 tttgtctgga atgccacgta taaaaatagt gtacgtttat ctaccaatac cttgcttcta
     8041 ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc ccatttagct
     8101 gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccttt cttaaaggaa
     8161 tataacggta ttatggtgcg tttagacgga tctgacggaa tgatcaatcc tttagagata
     8221 tttgccacac ggacgtatga tgaagcatcc agtttatatg acgatggatc agtcaaagat
     8281 ttgaagatca atgaagcagc gtcctatcaa acacacttag acaaagtggt ctatcaggta
     8341 cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac ctacttaaat
     8401 cgtttttatg aagagtatgg aacagtacca cgcggcttta cttcctcaaa ttcgcgttca
     8461 aatactgata cgcaagtcac agggaaagat ccagaagctt atccgacgtt caaagaattt
     8521 ttagcctttt taggacaact ggaactacca ggggcttccc aagagaaaaa gaatcggaaa
     8581 gaagaaatgg agtctatcgt ggaatcctta tgtgagactt atggcatgat ttttgatggt
     8641 catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat tgactctatt
     8701 acaggcctag atcgaggggt acaacaatgt cagatgtaca tggccatgac gttgatttgg
     8761 aatcaggcat tgattcaagg acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa
     8821 gaacaagact tcaaacgctt cttggcgatg attgatgagt gccataatgg tgcgacctgt
     8881 tctgcagtga aaagctgtgg gttgcaagta gtctaaaaac tattgtaata actcttataa
     8941 cgagaagtca aatgagtggg taacgccaca aagggcttcc actgatactc cgacatgcaa
     9001 caagtagttt atgcagacca acgttgtgtg aagctcggtg aagtcggctg aaggataccg
     9061 taaggtaaaa gaaatagtga ataccacgaa agttctctag aggtaaaggg tgtatccgat
     9121 aagtcgggtg tccaaaaaaa cttaatatgg tgagtatgta caaacgtact gacgaactag
     9181 cgaatgtact gtccgaaagg catagctcct agaaatggga gagacagtga gcaaaaaatc
     9241 actcattgtg ctaacttgtg gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt
     9301 acaggcgtag gagaagttag ggggcaaaag ctagcatcta agttaaggca tgtggataga
     9361 tataagggaa caggggaagc catcaacatg gagagtgcat actcgatgaa gtggtagtaa
     9421 gaaatatgtg aatgacaaat aacttacttt agtgatggtg aaagtagagt cacgactgat
     9481 gaaggttctg taatggaact ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc
     9541 cgttcaacca tgggattctc gtatgactaa aaaggtcacg agccaattgg agcgtgataa
     9601 accatgacaa aagaaacaag tccaaaagat aggaagttgc ggcatgcaga atattatggc
     9661 atgacggaaa tcttcgataa gatatactcc aaaagtttga agggagataa tttcaagaat
     9721 ctaatgcaga ttatcatttc tgataacaac attgtcctag catatcgtaa tatcaagaga
     9781 aatcacggta gtgtgacaag aggagaagat ggagtaacga ttaaagatat tgaaaatctc
     9841 aaccgtaatg aattcgtaga aattgttagg aagcgattcg cacattataa ccctcggaaa
     9901 gtaaggcgtg tagaaattcc taaatataat ggtaaattaa gaccgttggg tattccatca
     9961 atatgggata gaatcgcgca gcaatgcatc ttacaagtat tagaaccaat atgtgaagca
    10021 aaattcaata atcacagcta tggtttccgc ccaaatagat cagcagaaca cgcaatagca
    10081 gattgtatgg cccgaatgaa tcaaagtcac atggaatatg tggtagatgt agatatcaaa
    10141 ggtttcttcg acgaagtcaa tcatactaaa ctcatgagac aaatctggtc tatgggtata
    10201 cgagacaaac agcttctctt aatcattagg aaaatgttaa gagcacccgt agtacttcca
    10261 aatgggaagt tgatataccc aacaaaagga acaccacaag gcggaatact tagcccatta
    10321 cttgcgaaca tcaatctcaa cgaatttgac tggtggatag caaatcagtg ggaggaaaaa
    10381 gatgccaaag agctaaaaca tcattttagg aaaaatggag cttgggataa aggtaatatc
    10441 tatcacaagc tcagaaagag tacaactcta aaagaaatat acctagtgag atacgcagat
    10501 gattttaaaa ttttctgtag aaattataat gaagcgaaaa aagttttcta tgcaactaaa
    10561 atgtggttac aggagcgtct aaagttacca atatctgaag aaaagtcaaa aatcacaaat
    10621 ctttcaaaag aagaaagtga atttttaggc tttacgatga aattagtgac caaaggtaac
    10681 aaaaaggttg ttaatacgca tataggtaca aaagcacaaa gcatgattaa agtgaaacta
    10741 aaaaaacaaa tcaaacgaat tcaaaaaagt cctaatagca aagatacctt tatctatatt
    10801 ggagtctata acagtatggt tattgggatt cataattact tcaaaattgc gactcattgt
    10861 agtgtagcaa tgcgaaaaat tgcaagagaa ctcagaccaa gttttgaaat acgactacga
    10921 gaacaaggaa tctcaaaaga aggacaatac ggaaagtacg aaggatacaa gccttaccta
    10981 cggtcaggac aaatacgata tataagaggg tttccaatat taccagtagg gtatattcaa
    11041 cacagacatc cgatgtgtaa aaaaagaaat attaataaat acaccgaaga aggtagggaa
    11101 gccattcata agaagcaaca aaccgtagaa gaatggaaga tatcgtggtt gtgtagcaat
    11161 ccagtgctta gtaagagagc aacgattgaa tttgcggaca atcgaatttc aaaattcatc
    11221 gctcaaaagg gaagatgtgc agtaacaggt gaggagctta tcttatctga aatgcattgt
    11281 catcatatta tcccttatca tgaaagcaaa agtgatagct atgaaaacct agtgattgtt
    11341 actgaggagg ttcatcgggt aatacacgct acacaaagtg aaacgattga ggaacttcta
    11401 aaatatctaa aattaaatcc aaaacaaaaa gaaaaactca atgagttgag attaaaagtt
    11461 ggtaacgaag aaatatctta ataaatattt gtaggacggg aaaacagaat gtcttttata
    11521 gacgagggaa cgccgtatgc ggtgaaagtc gcacgtacgg tgtggactgg gggaaaagac
    11581 ggagataata tcaaactctt acctatcagt atcgattaat ccagatttta ttgaaacggt
    11641 aaattatgtc acaaactttc aacgagaaat gcgtaaagta gaagcaatga ctgttttagc
    11701 tacacaatcc ccacaagaaa tggtgccaga aaatatgtct tctgataact tctcgaaact
    11761 gaagaaagtc tttgaatttt cttctacgaa gatctttatg cgtatggatg aatctatttt
    11821 acgtcatata gaaggattag tgggtaaatc aatgacgaac tcagaattag aatccattcc
    11881 tcaacaaaat caaggagacg ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca
    11941 ttttagtccg aacaaacgtc aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa
    12001 aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa agcggcgctt atttttgtgg
    12061 ttgtttggta cttctgcagg tttgattact attttgatca ctgtgttcgt gaccctattt
    12121 ctaatacttg cggcgggctc gattgataat tcggattcag attcaagcag tggtggcgaa
    12181 gcttttacag gagaatattc ggaaggctta ccgatttata aagaaatcaa aggaagaggc
    12241 cctttttctg atgagattgc gcaatatgcc gtaggtgcag cagtgaaata taaattatta
    12301 ccaagtgtga tcctttcgca atacggttac gaatcagcgt ttggtacatc ggcgtccgca
    12361 agaaatgatc tcaactattt tgggattact tggtttgatg gttgtctgtt tcctaaagga
    12421 actgcacgag gaattggtgg cattgaaggt ggctggtaca tgaagtttcc caactcaaaa
    12481 gctgcatttt cctattacgg ctttatggta gccacacaat ccaattttaa tgcttgtgta
    12541 ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggcggtta tgccgctgca
    12601 ggaattacgg aagatagtcc gtattataca ggttgtatgt caattatcac ttctaataaa
    12661 ttaaccgaat atgatgaatt cgccatcaaa cattggggtg aaggtggcaa taacaacggc
    12721 acgattactg gtgaatggac gaatccattc cctggcagca gtttagataa aagttccttc
    12781 agcggtggtc aattatttgg aacgaatcca ggcggtgaat tccgtccgaa cggcttccat
    12841 gatggattag attttggttc agttgatcat ccaggaagtg agattcatgc ggttcatggc
    12901 ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggcgcgtg tgtgatcgtg
    12961 attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac aggaaattct
    13021 cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg agatacagca
    13081 cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca tgcattcatt
    13141 gatgatggta catggatcga tccgttacca tttttgaata gcagtaaaaa atagatcagg
    13201 agaaaaaata tggaaaataa aacgttaaaa atcgtcttaa cagctgcaat tagcgttagt
    13261 ttaatcggca atattgcgtt gttttggcag ctgaaagagg aacaagcagc aaatacctat
    13321 aaaattgaag aagtggctaa aaacgcgtct gagaagctca aaaaaaattc ctctgaggca
    13381 caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag
    13441 ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg
    13501 gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga attaacggaa
    13561 aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctac agacgatgat
    13621 caccaacatg atgattacgg aagttactcc tttacattag gtgacgtgca aacttacgct
    13681 caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc
    13741 aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac tgtcactgaa
    13801 aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg ttaggaggaa
    13861 gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa gttatcttag
    13921 atggctggga atctttatga ttctcggctt tttgattctt gctagtccga tttatacgaa
    13981 tcagaaggta gatataccag atgaggtaag cacgaacgcc tttttaaaca cgaaagagct
    14041 gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat ttgcgatcga
    14101 tgatgaagat caaagcttgt taaaagaact ggctaatctt aattatcgcg tagaagtaaa
    14161 gacagctaaa ggggattatc aagcaatacg agccaaagtg attaaggtgt ctgatgatta
    14221 ttttgcagtg aagctaacgc atgtaccaga agaatatatc gccatgcgtt taaccattat
    14281 tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga tttattacgt
    14341 gcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac acgcgatcaa
    14401 ttacaaagtg aaaggataca agaaagaaca aaaagcagct caaaaacaaa tggaatcttt
    14461 ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc aattgcctta
    14521 tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc agcaagaaat
    14581 tgaaacatcg aaaacgcaac tgaaagatca agaagagatc attcaaaaac ttcaagaaaa
    14641 aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa aaatgaagcg
    14701 aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta aagcctatct
    14761 caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaaca caacaatatc
    14821 tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat ttgaagctct
    14881 atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag gagtatttta
    14941 tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa ccatttgtta
    15001 atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac ataaaacatt
    15061 taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg tatcaattat
    15121 agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt ctaatcgaca
    15181 aagttagaag gattttttat tgtctaaata cacaaataac cttaattttt aaagagcata
    15241 aggaaacgtg atatcacttt ctcacgaaag gtgttaggta ggctgtgcct accacgtttc
    15301 taagaaagat aaatgatatc aaaattttgg atttgtgtgt agatttgtgg gtaggatgaa
    15361 agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa aatcaaaaaa tgtgtaggga
    15421 tatgtgtagc tacacatatc cctacacata ataaatttgg aggaaaatat ggacttatta
    15481 attagaaata ttgaagaaaa gtatatta
//
LOCUS       DAXWLH010000059        15342 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXWLH010000059 DAXWLH010000000
VERSION     DAXWLH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.059"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            72..2132
                     /locus_tag="ACGE36_001402"
     CDS             72..2132
                     /locus_tag="ACGE36_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFF8383800.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2215..2784
                     /locus_tag="ACGE36_001403"
     CDS             2215..2784
                     /locus_tag="ACGE36_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383801.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2959..3765)
                     /locus_tag="ACGE36_001404"
     CDS             complement(2959..3765)
                     /locus_tag="ACGE36_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383802.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3903..5417
                     /locus_tag="ACGE36_001405"
     CDS             3903..5417
                     /locus_tag="ACGE36_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFF8383803.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5820..6803)
                     /locus_tag="ACGE36_001406"
     CDS             complement(5820..6803)
                     /locus_tag="ACGE36_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFF8383804.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6808..7455)
                     /locus_tag="ACGE36_001407"
     CDS             complement(6808..7455)
                     /locus_tag="ACGE36_001407"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8383805.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7540..8172)
                     /locus_tag="ACGE36_001408"
     CDS             complement(7540..8172)
                     /locus_tag="ACGE36_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8383806.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="ACGE36_001409"
     CDS             complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="ACGE36_001409"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFF8383807.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8736..9473)
                     /locus_tag="ACGE36_001410"
     CDS             complement(8736..9473)
                     /locus_tag="ACGE36_001410"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFF8383808.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9470..10459)
                     /locus_tag="ACGE36_001411"
     CDS             complement(9470..10459)
                     /locus_tag="ACGE36_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFF8383809.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10717..11259
                     /locus_tag="ACGE36_001412"
     CDS             10717..11259
                     /locus_tag="ACGE36_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8383810.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11276..12361
                     /locus_tag="ACGE36_001413"
     CDS             11276..12361
                     /locus_tag="ACGE36_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383811.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12373..13179
                     /locus_tag="ACGE36_001414"
     CDS             12373..13179
                     /locus_tag="ACGE36_001414"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383812.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13180..14007
                     /locus_tag="ACGE36_001415"
     CDS             13180..14007
                     /locus_tag="ACGE36_001415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383813.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14004..15077
                     /locus_tag="ACGE36_001416"
     CDS             14004..15077
                     /locus_tag="ACGE36_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8383814.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4929 a         2772 c         3133 g         4508 t
ORIGIN      
        1 ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag agtagggagg
       61 actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac gcttgtcgtt
      121 tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg cgcacgatgc
      181 tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg acacaaacca
      241 tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact attactcata
      301 ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat aataaagtca
      361 gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta tggacatcgg
      421 aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct acaaaaggtg
      481 atcagttaaa acagatcact gatattttga atcatgcttt gacagatata aaggacggtg
      541 gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa agaaagaaaa
      601 tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg ctgaagaacg
      661 cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat atcgtggata
      721 cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt gctttaaaaa
      781 caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc aaaggaaaag
      841 atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa tatgtaacaa
      901 aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca gtagatacta
      961 caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg
     1021 cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact gtcatcactc
     1081 attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg agtagtgtaa
     1141 atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca aatatagatt
     1201 tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg
     1261 gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga
     1321 ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc
     1381 gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt
     1441 tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta
     1501 caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga
     1561 ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac
     1621 atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa gaagaaagct
     1681 tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa
     1741 caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa
     1801 ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta
     1861 aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat
     1921 cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc
     1981 atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc
     2041 cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc
     2101 gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat
     2161 aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta
     2221 gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca
     2281 atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct
     2341 tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat
     2401 tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag
     2461 acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa
     2521 gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca
     2581 aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc
     2641 tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt
     2701 acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag
     2761 gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa
     2821 aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat
     2881 ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct
     2941 cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt
     3001 aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc
     3061 aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt
     3121 ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt
     3181 gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg
     3241 ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac
     3301 aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa
     3361 aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac
     3421 acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc
     3481 ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat
     3541 ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag ggatttcaaa
     3601 taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc agacctgatc
     3661 cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg tgattctacc
     3721 tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat aactccttca
     3781 tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga cttaaagaaa
     3841 aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa agggggatac
     3901 aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct atgagcgcat
     3961 gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa tctgccgaag
     4021 tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa gaatcttatg
     4081 atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg
     4141 ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac acatctaaat
     4201 catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga atcaaagact
     4261 cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa
     4321 tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat caaaacggta
     4381 tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca catcgtccgt
     4441 ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac gtccatgagc
     4501 gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat ggggaaacgg
     4561 tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa
     4621 tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg
     4681 atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt atcaccatga
     4741 ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat ccaactgtgc
     4801 agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca atcttggtcg
     4861 atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta tctgcagcaa
     4921 ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg
     4981 ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag acaatggaag
     5041 aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt gatggatgga
     5101 atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc atggaacatg
     5161 acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat tacacgatgg
     5221 gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag ccaattaggg
     5281 ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa
     5341 attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca gctcgatttg
     5401 cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga
     5461 gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg aaaaagctgc
     5521 cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt taacttggtt
     5581 ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa gagcgtggga
     5641 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacagcg gaagcagaag
     5701 aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
     5761 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc agcatgattt
     5821 tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat atattcatgc
     5881 gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat cttgccattt
     5941 tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt atactgatca
     6001 cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa tacaccaagg
     6061 tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg tccatcaacg
     6121 gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa aataacatcg
     6181 actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt tttatagttg
     6241 atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga tcctgcagta
     6301 acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt tcccacacca
     6361 cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag ccagtaaagt
     6421 gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata gtctagcgta
     6481 tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata accacctaga
     6541 ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact tttattctca
     6601 actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa agagactgat
     6661 tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat cagtaattcg
     6721 ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac agaaccagga
     6781 tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt ggaatgctgt
     6841 attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat ccgtttcttc
     6901 cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg atgccttctc
     6961 attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg tgactggttg
     7021 aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat gtttagtaat
     7081 ataataatct gtcatggact caggccattt tgctggttca tctgcataaa cagcactgac
     7141 catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga ttgcgccatc
     7201 caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca atacatcgat
     7261 gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag agacatacga
     7321 aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg caaaaatttc
     7381 atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt ttgcacctac
     7441 aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc aatagtaaca
     7501 ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa gagaagggtg
     7561 cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg cgacgattcc
     7621 ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag catttaaatt
     7681 tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat tcatcactaa
     7741 aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg aatctgcttt
     7801 ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc caatcaagat
     7861 gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc cgatgactgg
     7921 gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac ggatcatgct
     7981 gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc
     8041 aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga taaaacctaa
     8101 aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg taaagttttt
     8161 tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag tcagcatgac
     8221 ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc cacaagctaa
     8281 aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt ccagattacg
     8341 ctgagtgatc gggttttgat acatattcgt gttcatggca ggggcaatca attttggtgt
     8401 tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc cgtttgctag
     8461 cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt gttttgctag
     8521 ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat ggactgcgcg
     8581 cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt tagacataat
     8641 cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc ttttgtaagc
     8701 agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc cagtctaatt
     8761 ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac tgttccatct
     8821 ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa
     8881 ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag agattccttt
     8941 ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca ttcacgtaga
     9001 taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt gttagaagag
     9061 atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc cagttcttct
     9121 tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact aaaagcagga
     9181 gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt tttcgttagt
     9241 tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt tatattggag
     9301 tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt atccggattg
     9361 gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca
     9421 cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt catttatggt
     9481 cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta gatgctgttt
     9541 caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc tgcaaatccg
     9601 ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa tcaattccaa
     9661 tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg attcccccag
     9721 cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga ctgcttagct
     9781 gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa gcttttgttg
     9841 tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt tttagccatg
     9901 tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca
     9961 gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact tttttatcgg
    10021 aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac cctgctgtac
    10081 caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac cagtctcctg
    10141 gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca aaaccagtag
    10201 caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca gttccggata
    10261 aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg ctcattgcca
    10321 atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca ttttcagcca
    10381 actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt attacctgaa
    10441 aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt atcataccat
    10501 aaagcctact cttttattct tgttcttccg taaatttaga caaaaattag gtgaaaaata
    10561 aagaatcaaa caactagtat ttattccaaa agataaagaa atattcagca aaaaaaattt
    10621 tacaaagggt tgcctttttc aataaatttc gtatactaat acgagtggta gtaaacagga
    10681 agtttactat tagaaaactt cgattcggag gaaattatgg aaattggtga gaagttaaga
    10741 aatttgcgca tccaaaagaa tttgacacaa gaagaactag gcgaacgcac agatttgtca
    10801 aaagggtata tttcccagtt ggaacgagat ttaagttcac catcaatgga aacattcttt
    10861 tctattcttg aagtcttagg tgtgactcca gaagaatttt ttcatcaaga agcagcgaat
    10921 ctgcaagtgg tttattcaaa agaagatcat acggtttatt tcgatgaaga aaacgggtat
    10981 gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt cttgctgact
    11041 tttgaaggtg caggagaata taagacgttt gaaccttcat tatctgaaac gtttattttt
    11101 gtattagaag gggaattaga gttaagctta ggagagacgg tctatgtggc aaaagcagga
    11161 cagtcgatct attatcaagc gacaaaacaa catcaattaa ggaatcatct caaaaaacga
    11221 acaaaagtat tgattgttgc aacgcaatca tatttgtaat atagggggta aaaacgtggg
    11281 gaaaccaatc atttcatttg atcaagtagt gaaacgctat gatgatgaaa cagtattaaa
    11341 aaaagtaagt tttgagatcg aacaaggaaa attttataca ctgcttggtc cttccggctg
    11401 tggtaaaaca acgatcctac gtattatagc tggattcacc gaagctacag aaggggacat
    11461 ctattttgaa gggaaacggt tgaatgattt gccagctaat aagcgacaag taaatacagt
    11521 cttccaagat tatgcacttt ttcctcatat gaatgttttt gataacgttg cttttggatt
    11581 gaagatcaag aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat
    11641 ggtccagctt cctggatacg aaaaacggga gattagtgaa atgtctggtg gtcaaagaca
    11701 gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa gtattgctat tagatgaacc
    11761 gttatcggcg cttgatttga aactacgtac agatatgcag tatgaactgc gggatctgca
    11821 acaacgatta ggaatcacgt ttatttttgt tactcatgac caagaagaag cgttggcaat
    11881 gagtgatgag atttttgtca tgaataaagg gaaaatcgtc caaagtggta cacccgtaga
    11941 tatttatgat gagccaatca atcatttcgt tgctgatttt gttggggaga gcaatatcgt
    12001 ggacggtgta atgatcgaag ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt
    12061 cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag aggatttgac
    12121 gatcacgaca ccagataaag gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg
    12181 tgtccattat gaaatcatct gttatgatga acagcaaaat gaatggatgg tccattcaac
    12241 gaaaaaagct aaagaaggaa gcaaagtagg attggccttt gagccagaag atatccatgt
    12301 catgcgtttc aacgagtctg aagaagaatt cgatgcacgt cttgacagct acgaagaata
    12361 ggggggacaa gaatgaaaac aatgcgcagg atctattccg tcccttatat tttgtggctc
    12421 ggtttattcg tgatcgcgcc tgtattaatg atcatttatc aatctttttt tgatatgaat
    12481 gggcaattca cgctgaataa ttatctatct tattttacgt cggggacatc gctttctatg
    12541 acgatcaatt ctgtatggta tgcatttttg atcacgtttt tcacgctgat catcagttac
    12601 ccgactgctt attttttaac aaaactgaag cacaagcaat tatggctgat gctgattatt
    12661 ttgcctacat gggtgaatct gctgcttaaa gcttacgcat ttattgggat tttcagtgtg
    12721 cacggcagcg tgaatcattt ctttgatttt atcgggatcg ggccgaaaca gatattattc
    12781 acagacttca gttttctgtt cgtggcaacg tatatcgaga ttccatttat gattatgcca
    12841 attttcaatg cattggaaga gatgaatcct tctttgatca gtgctagtag agatttaggc
    12901 gcgaatagct ttgagacctt ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa
    12961 agtggtgtcc aagcagtatt tattccttct ctttctttat tcatgctgac tcgtctgatt
    13021 ggcggaaatc gggtgatcac tttaggaacg gctatcgaac agcatttctt ggttacacaa
    13081 aactggggga tgggttctac aatcggtgtg attttaatca ttgctatgtt catcgtgatg
    13141 ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg
    13201 tatctttatt tagggatcgt ctttttattc ttatatgcac cgatcttcta tctgattttt
    13261 tattccttca atgaaggagg aacgatgaac agctttacag gatttacttg ggaaaattat
    13321 gaagctgttt ttgaagatac acgtttgatc attatcgtat tgaatacgtt catgctggct
    13381 ttcttatctg cattgatcgc cacgatcatc gggacatttg gggctatggg aatctattac
    13441 acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga ataacatctt attagtctct
    13501 cctgatgtta ttatcggtgc tagtttcttg attttcttta cactactagg attcggatta
    13561 ggttttcaga gtgtattgct ttcccatatt gctttcagca ttccaatcgt cgtcttgatg
    13621 gtcttgccaa aattgcagga aatgaacgat tcaatggtcg atgcggctag agaccttggt
    13681 gctaataatt tccaagtagt caaaaatatc attttgcctt atctttctcc aggaatcata
    13741 gctggatatt ttatggcatt tacctattct cttgatgatt ttgcggttac atttttcgtc
    13801 acaggaaatg gctttacgac gctctcagta gagatctatt ctcgtgcaag acaaggaatc
    13861 agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt tctccatgat attggtcatt
    13921 ggatactact ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta
    13981 cgcgaggggg tggcacggct gcgatgaaaa aattacaatc tctgttttta ggtattctca
    14041 tcattattgc ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg
    14101 gagcaaaggt acttaccatc tataactggg gagattatat cgatcctagt ttgatcacga
    14161 aatttgaaaa agaatatgga tacaaagtga attatgaaac atttgattct aatgaagcga
    14221 tgttcacaaa aatccagcaa ggtgggacaa actacgacat cgctatacca agtgaatata
    14281 tgatccaaaa aatgatcaaa gaaaaactag tacttccatt agatcattcg aagatcaaag
    14341 gattaaataa tattgatcca cgatttttag acttggattt tgatccagat aatcagtatt
    14401 cgattccata tttttgggga actttaggga tcgtatataa cgataaattc tttgatgcaa
    14461 atcaaattaa gcattgggat gatttgtgga agccggaatt aaaagacagt ttgatgctga
    14521 ttgacggtgc acgagaagtt atggggcttt ctttgaatag tttaggctat tctttgaata
    14581 gtaagaatat ggcacagctg acagaagcag caaataaatt gaatcattta acaccaaatg
    14641 tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg
    14701 cagtgacatt ttctggcgaa gctgccgata tgatgtggga aaatgaacat ttacattatg
    14761 tgatcccacc agaaggatcc aatctttggt ttgataatat cgttatgcca aaaaccgtga
    14821 aaaataaaga aggtgcctat gatttcatca actttatgct agaaccggaa aatgcggcac
    14881 aaaatgcaga gtatattggt tattctacac caaatgaaga agcaaaagcg ttattgccaa
    14941 aagacatttc gtcggacgaa caattctatc catctgatga cacgatctcc catcttgaag
    15001 tctacaaaga tctaggatcg aaatacctag gcatctataa tgacctattc ttagaattca
    15061 agatgtatag gagataatgg caaaactcag taagaatggg gacttatcaa cgttctaaag
    15121 agctaaaaat cgtccttttt ctatctgagt tttccaatct tctaccaaaa gcggatgaaa
    15181 cgccgctttt tttgtgttat tttatttttt ctaccacgtt tttctaccag agaaaagtgg
    15241 acaaaaccat tgtaaaatag ggcccgagac gggctagtaa atctaagatt gaaaaagtta
    15301 cagaaggata aagtaattgg aaactatgca aatatactta ac
//
LOCUS       DAXWLH010000060        15332 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXWLH010000060 DAXWLH010000000
VERSION     DAXWLH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.060"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(12..1376)
                     /locus_tag="ACGE36_001417"
     CDS             complement(12..1376)
                     /locus_tag="ACGE36_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8383815.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1373..2932)
                     /locus_tag="ACGE36_001418"
     CDS             complement(1373..2932)
                     /locus_tag="ACGE36_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383816.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2988..5468)
                     /locus_tag="ACGE36_001419"
     CDS             complement(2988..5468)
                     /locus_tag="ACGE36_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383817.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5452..5787)
                     /locus_tag="ACGE36_001420"
     CDS             complement(5452..5787)
                     /locus_tag="ACGE36_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383818.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5801..6835)
                     /locus_tag="ACGE36_001421"
     CDS             complement(5801..6835)
                     /locus_tag="ACGE36_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFF8383819.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6925..7605)
                     /locus_tag="ACGE36_001422"
     CDS             complement(6925..7605)
                     /locus_tag="ACGE36_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8383820.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7889..8992)
                     /locus_tag="ACGE36_001423"
     CDS             complement(7889..8992)
                     /locus_tag="ACGE36_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFF8383821.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9621..10091)
                     /locus_tag="ACGE36_001424"
     CDS             complement(9621..10091)
                     /locus_tag="ACGE36_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFF8383822.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10421..12061
                     /locus_tag="ACGE36_001425"
     CDS             10421..12061
                     /locus_tag="ACGE36_001425"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFF8383823.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12215..13537
                     /locus_tag="ACGE36_001426"
     CDS             12215..13537
                     /locus_tag="ACGE36_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFF8383824.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13605..14039)
                     /locus_tag="ACGE36_001427"
     CDS             complement(13605..14039)
                     /locus_tag="ACGE36_001427"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383825.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14104..14940)
                     /locus_tag="ACGE36_001428"
     CDS             complement(14104..14940)
                     /locus_tag="ACGE36_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8383826.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(15131..>15332)
                     /locus_tag="ACGE36_001429"
     CDS             complement(15131..>15332)
                     /locus_tag="ACGE36_001429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383827.1"
                     /translation="DFTVSSLNGVLLPTPRSQVTIFNKHEKLFFEIVPCDSELNGRPT
                     TSFLNGVQKLASAVISSTKRKL"
BASE COUNT         4477 a         3096 c         2775 g         4984 t
ORIGIN      
        1 cgtgatattt ctcacagtct tttatctact ctattgctat ttgctctctt agctagtact
       61 ttaaaagcta actcattgag gtagtgaaaa cgagaggtgt gttcagacag aaatccaaaa
      121 tcctcaactg ctagctgtgg aatcgtagtt ttgtgtttca cgatatccgc aagcaggttc
      181 aaatctgata ataagaattc ttggcttatc agctgtccgc caattatttc taatgtatcc
      241 ggatggtaga tcagctttgc atttactgtt ttgttttctt catcgtattg tggaaataga
      301 tcaaattctc ctgaacacga accggtattt tgttccaaga atggcgcttc atcttctgtc
      361 agccctacag ttcctaaaaa gtagtctata atgactgctg ctgttatctt ttgtctgagg
      421 tcgtaagcgg ttggctgacc actcaaagtt agcgctactg ctcttgccat atgtacagct
      481 cgagtaacta aaggtaaaaa gagatgccgt ttatttttgt caaaactgac tgggacgata
      541 tctccaatcg catagatgtc cggatcactt gtctgcagat attcattgac tatcacagtc
      601 tcatcctcat acaaagccac tttttctttt aacaatgtcg tattaggacg agtttgggca
      661 gaaaaaatga ccatatctgc ctttattgct tgagatagaa gttgtacgga tactactttc
      721 tcttctgatt ttttccaatc gatcaagaat tcatttggat aaaaattgat ttgcttagtt
      781 tgcagctgat tttgaaaact gttgatcata tccttatcga aatatcttga taaaacattc
      841 ggcatataat caactagatg aataatgtat cctttatttc tcaaagcatc tgctaattcc
      901 aatccaatat aaccagcacc gaagatcact acttcttttg cttcttttaa agcggcaagt
      961 acttgcttag attgcactag atttttatag gtgtagacat tttcagcttc aatttgttta
     1021 tacaaggtga caggagagga acctgttgct acgattaatt tatcataggt tatggtctcg
     1081 ttttttcctt ctttggatgc gaggatgagc tgatgtttgt ctggatcaag ttcagttact
     1141 ctccattcgg tgatcagttt tgccccactt tcttgaagag tccgcatgtt atgggcaact
     1201 tcagctaaac tacgtatctt tccttttaaa taaaggttga ttccatttga aacatagcct
     1261 agtccatctt tatgatcttc atcgatcagt aaaacttctg ctttcggttg aagttttctt
     1321 agatttaggg cagctgtgat gcctgcatgg gaagcaccaa tgatcacgat tttcatgttt
     1381 tattcatcct taaattcaat ttttctaaat aacattcttt taaaaattgg ttcaaatagg
     1441 ggaaatcata attatattcg ttgctagtga atacaaaaac tttagcggac ttctgtgacg
     1501 ggtactcttt caattggcta gtcaaaatga tatcatactg cttttctttc tcgtattcga
     1561 ctaaatcata tttgatcgta ttcagcaatt gttcctggat gatttctttc acgatatccg
     1621 ccattagcct ttcataagga agatcataag caatttgcag acgtaattct aactgcttga
     1681 tcagcgtccg caaaatagat gcatagcgag caaataattc aaagcgggca ttttcctcgt
     1741 ctacttcagt aaaatcaaca atagcaaatg tttgtctgac caatctttca cataaaacca
     1801 ggaattgttt atcttctact tcagttaacc gctgttttaa tccttcttgg ccaaaaatat
     1861 tcatccatcc tctaaaatag gcaaaatgag cgtgattttg catcactgtc aacgcaatct
     1921 tttgcgaaat ctcttctgga aaaacataaa aagagaaata ttcttctatc gattgtttaa
     1981 acgttcgatt caattgagct actacgtcgt ctttgtattt ttcgcttatc aaaaactcct
     2041 ggacatgcga gttatgtgta tctaatgtaa agttactgat catgaaaaga taaaaaacca
     2101 acgtttcatt ttgattccaa ggaaaggcat attgaaagaa gtaacgggtc aaaaatgtat
     2161 ggaggtccaa taaaaattgt gtcgtttgaa acaatccaac aggaagattg ctataatctg
     2221 gtttggattt tttcgtacag cgcattcgct tcttcataat ggaaagccat atttcaaatt
     2281 tcaatcgtcc ttcttctgta atagtaaaac taaactcttt ttgcagcaga tctaaaaaag
     2341 tgtttcctga taaatcactg tattcttttt tgttttcaag ataaggacgt ccaaaccaaa
     2401 ataattggaa ataaaaataa caaatttgac tttctggacc ggtcagccgc ccgcttttta
     2461 ttttcagatg aaactcattt aaagaatcat ttaattcctt cactctgcga gacaaagtag
     2521 ccgaactgat attaagttcc atgacaagtt tctgccgatc aaatgagcca ttttccaaca
     2581 gataaagaag tatctgatat tttgtagaat agcgcaagta gcaatgataa taaacatcga
     2641 ctgctgctat atttccatgt ctgattaata aaatattccc ttcttggata acaatttcca
     2701 attgtttgat attttccaag ttagcttgta aataaaacag atcttctttc aaggtagccc
     2761 gatcaactga taagcgggta agtacgttat taacggaaat ctctcctttt tcctctaata
     2821 aaagttggaa aagtttgatt tgccgttgtt catttttttc aagcaaactg aaaagttcca
     2881 agtccactcc tccaagcatt tctgatttta acagcagaag tttccttctc atccttgaaa
     2941 aagaggatct gtgacagtta tcacagatcc atacaaattc taatcactta gctggttggc
     3001 ccagctgtca gttcccatgt gaccatgcct tgatacgttg cgccaggttg agcattcgta
     3061 ctgctattta cctctaagag taaaccatcg gtatcgggaa agtcaacgag aacctctcct
     3121 ttagctgttc catcactctc ataaatcaac gtgttttgac cttgctgaat caattgtttt
     3181 gatccagaat gattgaagta taattttgtt ccgcttaatt cttgaccttg atcattctta
     3241 aataaagaag tcaatgtccc tgtcagttgc caaggagacg tgcttggaag ccgagtatcc
     3301 cgcacatcaa aagaaaaatc gccttttgct tcataataac ttgtcttcaa cggtttgggg
     3361 agatcgttac caaatgtaaa atcatctggt acattaacta atgttaattc ttgtttgggg
     3421 acaagaattg ttttagcaga tgtttggaca actgggttag gaagcttagc accatctgct
     3481 aatgtaatat tgctggaaag tacagctgcc tttgtcagta tgctgacgga ttgtacagaa
     3541 gtatctacag tatttaattg atagttgtaa gtaagatgaa tggtatctcc tattttgctc
     3601 aatagatctg ctgggacaga aactccttgt tgaatgccgg tatctgcgat agttgtacca
     3661 tttaatttta aggaaccatt gatataagta gtattttcag gaagtgagat cgtgattttt
     3721 ttgtcggtta tcgcatcccc agattttgtt aacgtgaatg tcacattttc ttgaatcgta
     3781 tctcctgata gagcagacag gggatctcct gtctcaggtt gtctgctaag gttttttagc
     3841 tgactatctg cactcacaga tggaaaatta gccactgtta tttgaggaag agtggtgatt
     3901 ccagcttgtt cagactgcca tttgtacgca aaagacggat acatgttagt aagtacatcg
     3961 tccaccaagc gataatcaaa ataatattgc cctgaagcca atgcaggtag tgtagaagta
     4021 gctgtattta cacccccgtt atttgattgg atcgtttgga aaagttgatc tgtccatcct
     4081 gtcgctaatt tttgggttcc tttcgaataa agcctgtaat ggatcgaacc cttttttagt
     4141 tcagttgctg accaattggc cgtgacttgg tacgatgcag aagaaaggag ctttccccac
     4201 tgactatcgg ctgttaagtt tgttggtaaa accgttccgt ctagcttgta ctgctccaat
     4261 acctttaaag caacaggaga aagcaatccg ccttgcgcat ctttcccact gtacacgata
     4321 ttggctgtct gattcaatcc acttgtgctt gctggagtat gagtcagtat tgctccttgt
     4381 gtaacgtttt ttcctgtaaa atagttgact acgcgatcaa agcgactaaa tggcaactga
     4441 tcaaaacgtc cttcagtcag ccccatcgtc aatgtccgac tattggtaga gcctggaagg
     4501 ttctggttat ttccatccac gtattctgtg gtatcagcaa atgtattata ttttcctgtt
     4561 gaatcacttg ttgagatgta actattccac gtatttatct ctgcacttgc agaaaatgca
     4621 tatgctcctg gattcaagtt tccgccagtt aatttaggat aaaatctacc tgcagctgga
     4681 gctaaaatcg aattgaacat acgatcttgg ataatattat tcgaatcata aaaatcaaca
     4741 gaaaaaacgg cctttttaga catagaaggt tgtaatttac ccttggtcaa aattttgttt
     4801 ggactcgtat catatttatc tccgttttct gtcatcttcg cgacattagc tgtaacatca
     4861 aattgaagcg ggatatcgtc cacgttgaat tcgcctcgcg taataatagc aataatatct
     4921 gccaatagag tatttgtatc ccacgacccc atcccaaaac gcaaggtatg gcttcctgct
     4981 gataataaac taccgctagt aaccttgatg ggggcaccgg caatatacaa gtaggcgttg
     5041 ttccagtcaa ttgcttttaa tacatcactg cttgatacat gttccggcaa attgatgatc
     5101 gccacaaatg caaatccttg tgcagtgata ttcaccaaat caagtacatt tgttagtttc
     5161 acatggcttt cccaatgaaa acggaccgtg ccacctttcc aaggatcatt tgaaccatcg
     5221 ttcaaataag tagcagtcaa tttaggcaat tggttagccc cagttagttg gacaagcttc
     5281 ccgccattga ctgcactacg tccaccattt ggaggtacga tattttcata cgtgtatgga
     5341 tcataagaaa tggcaggagg cgttgctcca acagcttctg ccgaaggtga aaagataaat
     5401 atacttaaaa caaaaagcga agtaaaaaag aaggagcgaa aaaatgaaaa tttattttgt
     5461 agcaacatgt tcatcctcct tttcctttct tctccataac aacaaccacg caagaagcca
     5521 taattctata ccaatgatca aataaagaaa acggatcttt tcttcattcg tctccgggaa
     5581 tacgccatgt tgattagatt ctttataaat cggtagctgt gtctgctgtt ttgatgcgcc
     5641 attgactgaa ccacttggat gcggaactgg acttagatca taaaatgtaa tcgtacctga
     5701 acttttggta tctgcttgga ctggctcttg ccagctgaag aacagcaaac ttgcaataat
     5761 ccctcccaat aagatcgatg ttttcaacga aatcacctca tcatgttgtt tctccttttt
     5821 tctttttctt gtggacccac aaaaacagca aaatcaacca taacactaga actgcagcaa
     5881 ccaatcctgc ataaagccat gggaaagatt gttcttgtac tgcaaaaacg gaattttcat
     5941 ttactttttg tgctttttct tttttgatcg taaagttttg tgtaaatcgc cattcattac
     6001 tgtcgcctga ttttgctatg atttctgcgc ggtaatcgcc ggcttttgct ttatcaccag
     6061 ctaaatcaag attcaagtga aaattcgaat ttggtgcaac taaaaattca ttagacgtat
     6121 tttcaatata aagctgattt tcataaaaga tcttgctcgt aaatatcaaa ttgctgataa
     6181 tcctaggctg aggattttgg aaacgaagct gaacagttgg ctttgcgttg acctcgtcca
     6241 aatctcctga taaaagtttc agatccggtt ctatcgtctc ttgactttct tgtaaaacga
     6301 ctgcgagatt ataagcaata cgatttttga tttgtgctcc ttctgtagca ttttctgttt
     6361 cagcttcttc tttaggtgaa acatgaatac ccgctaatac acgtccctta aagctttttg
     6421 ctgggatttt cacttctata ggtatagttg tttctgcatg tgctgataca ttgatttcct
     6481 ttttcgcaat gagcaaaacg tcacggacat caaacggcaa tgtctcatct aacgttgggt
     6541 ttttttgact ataatcgatc gttccaccat cactcgttac cgccggattg acagagacct
     6601 caaatgttcg ttcttcatct gaagaattgc tgataattac ttctagtgtc tgattttgtt
     6661 ccggtgccac taataaatca aaataaccga ttgctttatc tatttggttc tctggaaata
     6721 ccggtttgat tccaaagtct cctgctgaat cctttgcttg gctgattgac ggaaaaatga
     6781 aaaatagact taaaatagaa aaaaacgaaa caaacagtcg tttactttga ttcattgtcc
     6841 accacatcct ttcaacaaca actgcggtaa aaaatccttc acctatagta ttttcaccgc
     6901 agtttgttga atttatttca tactctatgc tactgggcca gccacgaggt tccaagtcaa
     6961 atcagccgtg aatgttccag ctgctgcttt cggcactttc agtgtgatat ttgctggatc
     7021 ataaacagaa agccatgttc ccagaccttg atcttttgct gctgtaaata ttggtgtcgc
     7081 tgcacttgct ttactcaacc cagttacagt ttgactagtt ggtccagtgg attcgttatc
     7141 tgacgtactt ttaacagtgg atgttccaaa atctaattca gcgccttgca aaacttgtga
     7201 accgtttaca aaatctgaaa ccgaaactgt cacgttccac ccttgtgctt ggccatctgc
     7261 acctacacct cgacgatctg atacttggag ataaggtgta tcgttttttg aagcgtctac
     7321 ttgatacgtt tttgttccgc tttcaatctc gtgtgtacca aatggcaatt caggaactac
     7381 atccaatgtt aaagaacctg tctggccagt tgctggattt tccggatctg ctggctctac
     7441 tggttttgaa ggatcagtag ggttaagagg ctcaacggta tttgttggtt cagtaaaggt
     7501 aatatctcct tttgaagtcg cttgaccctc agcaaaagct ggtgttgcca agccgcctag
     7561 aagaagtgtt gaaagtaata atgtcccaat catgttcttt ttcataataa atcactcctt
     7621 tttcttagtg acctaagtat agtagagaca gtatgcaaaa aatcctcatg aattcatccg
     7681 cttacttgaa attatgaccc tttttgtata aaagagaaaa aaacaataaa atcgtttaca
     7741 tgaatattct tcctttttta ttaagtatga aaaaatattt caattttatt ttttcgtttc
     7801 aaattattct attagtgaca tccttgaact ataaaaatag gagtacggca tcatctgccg
     7861 cactcctatt tttttcgtca tttatttctt aaataaaacg attctcacgc aattttattt
     7921 ttttgatatc ttctactgtt tgtgttccag ctaattgcat gaccatttcc aattctgttt
     7981 taaagaaatc aaacacttga tggactcctg tacttcctcc taaagacaat ccataaataa
     8041 cgggtctgcc aatagcaaca agatcggcac ctgatgcgat tgctttgaaa acatgttgtc
     8101 cacgtcgtac accgctgtca aacacgattg gtacacgttt atctactgct tctgccacat
     8161 attgcaacga atcaaacgca gcaggccccc catctaattg acggccacca tggttagata
     8221 cccaaattcc accagcacca gattctaatg atcgatagac atcttcttct gattgaacac
     8281 ctttcacgta gactggcaaa tcagaatgag cagcgataaa ttctacatct tttggactaa
     8341 gtttttgttt ggaagattta tatacagcat ccatcgtttg gcctactcct gattgataag
     8401 cctgaacgat cggcattggc agcgggaagg taaacccgtt tctgcggtct gtttcacggt
     8461 ttccgccaac tgttgcatcc gctgtcaata cgattgcttt tgctccatta cgcttggcca
     8521 cttcaagaat atccaaattg attccattat ctttgctcat ataaaattga aaccattgcg
     8581 gtgctttctc cccgcctgct tctcttatct cttctaaagt acaagaagcg taagaacttg
     8641 ctgtatagat cgtcccaaaa tctgctacac cttttgcaga agctttctcc gccttcacat
     8701 gagctaatcc atgagcagca actggtgcca tgatgattgg tgcagtcagc atttcttcgt
     8761 caaaatgtgt agttgtatcg ggtaattcta catctcttaa tacatgagga ataatcaaac
     8821 gatgattgaa tgcccgttca ttttcttgat aagtaaataa gtcgccagca ccactgctga
     8881 tgtaaccata accaccagct ggaatcactt gtgaagccgc gatttctaaa tcttccatat
     8941 tgataaaatc tactattcct tcatttgtac ctgcttgata tgttttttcc atctcaatgc
     9001 cttctctcta taaaatttga atgatgaaca atatgatagc gtttacactt tttatattac
     9061 tcttcgacat tgatacaggc aaataatctg tttacatttc agccattcca gatgtcacta
     9121 ctctggaaga aatgattgta ataaaaatag ataaaaaacc gatgggactt tgacaaaagt
     9181 agtgtcaaag tccctttttt tgaataaacg gtgttcaaaa gccattttct cggtattaag
     9241 tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tactcgaaac
     9301 taagcgactt tttcacaacc tctcgataaa acggtgttca atcagctgac cctcggtatt
     9361 agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg
     9421 tcagaacgtt gacttcacag tctcttcctt aaacggtgtt caaaaacttg catctgcggt
     9481 aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
     9541 tgatgcaggg caggtttttc acgacctctg attcacggtg tttctttttc atcaacattt
     9601 ttacgaaata ctgtggaaga ctattgaaat aagtgtggat tgtttctttg tgtttcaatc
     9661 gtttcttgcc attcttcatt taattttgga ttaccgtttt cttccttgaa ctcataaggt
     9721 cctaattcaa aaatcccaga cagtatttga ttcacgattt catatagttc agtgtgatca
     9781 gacacatgat tcttgaagat catctcatta tcaccacgtt tgaacctcac attcgggctc
     9841 tcatgatgaa cctctatgca tttttctcct ttttttaact gacggacttc tgaaaacatc
     9901 tcataaatat cttctccagt aaaaccttca gcaagcgcca gactgtccac aagaccgcct
     9961 actgtcagtt cagagattat tgcttgattg gaatctaatt ttaatgtata gcttgtcgct
    10021 tctactacgc cacatgcatt gcaaccgcct tctgatagtt cgctttttct aatcaattga
    10081 acttctttca ttttctcttc ctcctcttaa ttttctactt atgtattttt atcacctgac
    10141 ataggtgact cctatcaacg ctatcatgtg cttttttcct cagaaatcct atttatccat
    10201 tccatctatc ccttattctt ctacttttct ttctctagca tataagagat acgcatagta
    10261 ttcaagcaag gattgtcgat ataaaaagaa caaatactgc tattttgatt tcttttggct
    10321 atattacata taaataaaat ttatcatacc tgctcttttt gactatatca tcatctgact
    10381 tctatacttc gtttgtatta aaacttagga ggaatcattc atgcttacag ggttaaactt
    10441 attaaataat ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata
    10501 tggaatcact ggtatgcttc caagtactgt ccaaacactg gaacaacaat ctgttcaagc
    10561 ttatggtcaa tacttaagca aacaaacaga tttggaaaaa cgtatcttct taatgaatct
    10621 attcaatacg aatcggacat tgttttataa attgatgggc gaacatttag ttgaattcat
    10681 gcctgtcgtt tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt
    10741 gaaaccgcaa gatgctgcat ttctttcaat cgacgaacca gaaaacatca aagcaagttt
    10801 gaagaacgcg gcagatggac gagacattcg tttgattgtt gtgactgatg cagaaggaat
    10861 cttaggtatg ggcgactggg gtgtgaacgg tgttgacatc gctatcggca aattgatggt
    10921 ctatactgct gctgctggta ttaatccagc acaagttttg cctgtatcga tcgacgctgg
    10981 tactaataat gaagaattat tgaataatcc cttatactta ggcaatcgtc atgctcgtgt
    11041 agaaggcgaa acttactatg aattcatcga tcaattcgtt caatcagcta ccgaattatt
    11101 cccagaattg ttattgcatt gggaagattt tggacgagga aatgctgcag cgatcttaga
    11161 aaaatacgaa gatcaaatca ctactttcaa cgacgatatc caaggtacag gaattgttgt
    11221 cctagcaggt gtattaggcg gattgaatat atccggtgaa caattgaaag atcaaataat
    11281 tttgacattt ggtgctggta ctgctggtgt gggtatcgca aacatccttt tagatgagat
    11341 gatccgtcaa ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca
    11401 aggtttattg tttgaagata cagaaggatt gactcctggt caaatcccat tcgctcgcaa
    11461 acgctcagat ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcgat
    11521 ccatccgaca atcatgattg gtacttctac ccaacctgga gcattcactg aagaaatcgt
    11581 gaaagaaatg gctgctcata caccacgtcc gatcatcatg cctttgtcta atccaacaaa
    11641 attggcagaa gcaaaagcaa aagatttaat agaatggacg gacggaaaag cattagttgg
    11701 aacaggtatc cctgctgctg atgttgaata taatggtgta acttaccaaa tcggtcaagc
    11761 aaataatgct ttgatgtacc caggtttagg acttggtttg attgcttcaa cagctacacg
    11821 agtaaatgct gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac
    11881 aaaaccagga gcagctatct tgcctccagt agcaaaaatc actgaatttt ctcaaacgat
    11941 tgctgaaact gtcgcaaaat cagttgtcgc acaaaacttg aatcgtgaag aaatcacgga
    12001 tatcaaagaa gcagttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta
    12061 agatcgttat agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg
    12121 actgcggcaa aacagcggcc agccaactcc tgctcttttg tcgcttttct ttggcttttt
    12181 tcatatgata cagaataaga aaggatctgt aaaaatggca caaaaaacaa caactgcaac
    12241 tgcagttgag gaaaagaaaa aagaaggttt ttggtccaca aaaatcaccg gcgtaagttt
    12301 gccactttat ttgatcatgg tccttgtcct gatcattgca atggctttag ggaaattacc
    12361 gacgaatatg attggaccaa tcgcagtttt ggttattttc ggtaatttat ttcattttat
    12421 cgggacaaaa atccctatcg tcaaaagtta tttgggtggt ggttccgttt ttgcgatctt
    12481 cgcttctgct gcgattgcta catttgggat cttacctgaa tatgtagtga aatccacaga
    12541 aaactttgtc agcaatatgg gcttttggga cttttatatt gctgccttga ttactggtag
    12601 catccttggt atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc
    12661 acttatttca atggtcgtct ccttttttgc cgtaggactc gtgggcatgc ttatcggaaa
    12721 tggttttgca aattccgttt tatacatctc attacctgcg atggctggtg gtgttggagc
    12781 cggagccgtt cctttatcaa cgatttatgc aaatgtttta ggtacggatg cttcttctat
    12841 catttcccgc ctgattcctg cgacagcaat gacaaatgtc ttagctatca tcggtgcagc
    12901 attagttgct cgtgctggac aatcaatgcc aaaattcaac ggcaatggta aattaatgga
    12961 aaaaggcgat cttggcgatg gaaaacctcg aactgtgaaa ccttccattc cacagttagg
    13021 tgttggtttg atggtatcat tgactttctt catcttagga caaattggta actattttgt
    13081 tcctaaagtc catgcttacg catttatgat tattatcgtt gtcatctgta aaatcgccaa
    13141 cctattacca gagtattatg aagatgcagc tatcatgttc aataacttga ttgtcaaaaa
    13201 cttgacagca gctgttcttg ccggtatcgg tattgcgttg ctgaatctga atgtattagc
    13261 ttctgctttg acatggcaat ttgttgtctt atgtctgaca agtgttatcg cgatttcagt
    13321 tgtttcagga tttgtcggtc gtttgtttgg tttatatcca attgaatcaa ctattactgc
    13381 tggcttatgc aacaactcga tgggcggaac aggaaatgta gccgtcttat ctgcagctaa
    13441 tcggatggaa cttattgctt ttgctcaaat gggcaatcga cttggtggtg ccatcgtttt
    13501 gatcatctct ggtttcttga tgcaattact ttcttagtca aaaaaacaaa aatacctgat
    13561 catcaagtat cggcttggtg atcgggtatt tttttgataa agatttacag gattttcaaa
    13621 aatctttttt gcattttatc ggaatgataa ccgatgtgtt cataaaattg gtgtgcttct
    13681 actctgtatt ctgcagaatt aagccttatt gtcgttattc ctcgtttttt cgcttcttct
    13741 tctacgccag tcatcaaagc ctttccgatg cctctttttt cagcatattt agacacagca
    13801 agtgccataa tattgaacat tggatcgcta aaaatcgatt cataggactc cgcttgtaca
    13861 tagccagctg cttttttaga agtaggatct tcataaacca agaaaaaatt tttctctggc
    13921 tccttcagca gtttcttcat ttgtctcacc gtaacggtta atggtatatc ataccctaat
    13981 tgatccgcat tgatttcacg tagtgcttct gcatcttcta aacgcatttc ccgtaacatc
    14041 caaatcacca gccaatttct gattttttaa aatgaatttt tttatcatag tatactcttt
    14101 ttttcattta gaacaatatg tttcagtgat tttgacttgc gttagtctag attctcctaa
    14161 tgtttgtaag atgacaaagg cattgtaaca attgaccaaa agaaccacga ggacaattac
    14221 ttgaaaaata aaggtcactt tttttggaga caaaaccctg ctaaatcttg ctccccacaa
    14281 acctccaata acagcagcgg ggataacata aaacaacata gataaatcaa agcgcataaa
    14341 gctacttgtt aaagcaatcg ttaccaattt tgccagttga gaaaagaaaa tcgtagatag
    14401 agaatatagt gttgcttctt ttattggcaa tgcaaacatc agcatcaaca aagaaacatt
    14461 gactggtcca ccaccaatac ctaaaaaact agctaaaaag cccagaaccc ccccacatat
    14521 aaggtaccaa gtccatgacg tcaaatgaaa ttttggctga tgatgcttgg tgtagaagaa
    14581 cgcaaaaacc aaggtcaata ccgtcaaaaa aatttggatc agctgcacat gcttctcgtt
    14641 ttcaaataac tgtaaaaaaa cttcgaatac aatgttgccg actactcctc caacaacagc
    14701 tccactagat acccaaccga caatctgcca attcagtgat ttcccgcttg atacttgtcg
    14761 cacagtcgaa acaatggaca tcgtgaatac cgcaactgtt gagtaaaaag aaatccctgc
    14821 aacagaatgg gcgccgataa gatcaagaat cggtttgatc aacacgcctc cacccatccc
    14881 ggaaatagcg ccaactgtgt tcgccagaac gatcaccaca aaataaatca gaccaatcat
    14941 cttgtatttc ctttctcttg tttatatctt ttagcgattg ttactctttt cttttattat
    15001 acagtaaaag aaacaaacga ggcttcgaaa gcttttctac tttttaagga aatttagtgc
    15061 taaacgaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
    15121 aaatagctcc tcataatttt cgtttggttg aacttattac cgcagatgca agcttttgaa
    15181 caccgtttaa gaaagaggtc gtgggcctgc cgttcagctc cgagtcacaa ggaacaattt
    15241 caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga
    15301 gaacaccgtt taaggaagag actgtgaagt ca
//
LOCUS       DAXWLH010000061        15229 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXWLH010000061 DAXWLH010000000
VERSION     DAXWLH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.061"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE36_001430"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE36_001430"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFF8383828.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE36_001431"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE36_001431"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFF8383829.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE36_001432"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE36_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFF8383830.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE36_001433"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE36_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFF8383831.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE36_001434"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE36_001434"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFF8383832.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE36_001435"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE36_001435"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8383833.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACGE36_001436"
     CDS             complement(8054..8524)
                     /locus_tag="ACGE36_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFF8383834.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACGE36_001437"
     CDS             complement(8570..8995)
                     /locus_tag="ACGE36_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFF8383835.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACGE36_001438"
     CDS             complement(9062..9331)
                     /locus_tag="ACGE36_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFF8383836.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACGE36_001439"
     CDS             complement(9342..10937)
                     /locus_tag="ACGE36_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8383837.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE36_001440"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE36_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFF8383838.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE36_001441"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE36_001441"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFF8383839.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3948 a         3439 c         2699 g         5143 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaa
//
LOCUS       DAXWLH010000062        14995 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXWLH010000062 DAXWLH010000000
VERSION     DAXWLH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.062"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE36_001442"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE36_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8383840.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACGE36_001443"
     CDS             complement(322..891)
                     /locus_tag="ACGE36_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFF8383841.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACGE36_001444"
     CDS             1077..1979
                     /locus_tag="ACGE36_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8383842.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE36_001445"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE36_001445"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFF8383843.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE36_001446"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE36_001446"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFF8383844.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE36_001447"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE36_001447"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8383845.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACGE36_001448"
     CDS             8547..8732
                     /locus_tag="ACGE36_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFF8383846.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACGE36_001449"
     CDS             complement(8794..9990)
                     /locus_tag="ACGE36_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8383847.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACGE36_001450"
     CDS             complement(10160..11596)
                     /locus_tag="ACGE36_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8383848.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACGE36_001451"
     CDS             11889..12698
                     /locus_tag="ACGE36_001451"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8383849.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..>14995)
                     /locus_tag="ACGE36_001452"
     CDS             complement(12884..>14995)
                     /locus_tag="ACGE36_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFF8383850.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDRELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4378 a         3179 c         2660 g         4778 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actctcgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DAXWLH010000063        14628 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXWLH010000063 DAXWLH010000000
VERSION     DAXWLH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.063"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            39..812
                     /locus_tag="ACGE36_001453"
     CDS             39..812
                     /locus_tag="ACGE36_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383851.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(893..1462)
                     /gene="thiT"
                     /locus_tag="ACGE36_001454"
     CDS             complement(893..1462)
                     /gene="thiT"
                     /locus_tag="ACGE36_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFF8383852.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1528..1630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1636..2607)
                     /locus_tag="ACGE36_001455"
     CDS             complement(1636..2607)
                     /locus_tag="ACGE36_001455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8383853.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2701..4074)
                     /gene="glmU"
                     /locus_tag="ACGE36_001456"
     CDS             complement(2701..4074)
                     /gene="glmU"
                     /locus_tag="ACGE36_001456"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFF8383854.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4577..5395)
                     /gene="purR"
                     /locus_tag="ACGE36_001457"
     CDS             complement(4577..5395)
                     /gene="purR"
                     /locus_tag="ACGE36_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFF8383855.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5562..6386)
                     /locus_tag="ACGE36_001458"
     CDS             complement(5562..6386)
                     /locus_tag="ACGE36_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8383856.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6359..7039)
                     /locus_tag="ACGE36_001459"
     CDS             complement(6359..7039)
                     /locus_tag="ACGE36_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8383857.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7062..8000)
                     /locus_tag="ACGE36_001460"
     CDS             complement(7062..8000)
                     /locus_tag="ACGE36_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8383858.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8245..9096)
                     /gene="ispE"
                     /locus_tag="ACGE36_001461"
     CDS             complement(8245..9096)
                     /gene="ispE"
                     /locus_tag="ACGE36_001461"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFF8383859.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(9250..11823)
                     /gene="mprF"
                     /locus_tag="ACGE36_001462"
     CDS             complement(9250..11823)
                     /gene="mprF"
                     /locus_tag="ACGE36_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8383860.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11964..12770
                     /gene="proC"
                     /locus_tag="ACGE36_001463"
     CDS             11964..12770
                     /gene="proC"
                     /locus_tag="ACGE36_001463"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFF8383861.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12849..14405)
                     /gene="rny"
                     /locus_tag="ACGE36_001464"
     CDS             complement(12849..14405)
                     /gene="rny"
                     /locus_tag="ACGE36_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFF8383862.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         4259 a         3179 c         2540 g         4650 t
ORIGIN      
        1 aataaaagag attaagagga aataagagga ggaacatgat gactgagtca gtttatgtac
       61 atattgatac cacaagtaat gctgttttaa caaaagggct gactgctact gactttgcaa
      121 acagtatcgt gcattttcct caaaacttgc tcttgcttga tccatctgct tctgctggag
      181 aatatgaatc ccatacaggg ctgaaagtaa tccgtggtac agaaaatatt cagcgtttct
      241 tttcctcttc tcgtaacagg cgtggattcg atgaattgaa gtggatcgac tttactgatt
      301 taacgatgct gaaagaattg acacctttag aaatttcaga attattatat tttggtcata
      361 tgaaaacaca tcttcattcg ccgttttttt ataaactaca gaataatttt gtatacttcg
      421 atcttaatga tcagctgaat cggatttatt atcgctattt agatgaattt tataggattt
      481 ttgctagtaa attaagttcg atcttgtcag ataagatcaa tgtaaaaaaa tcttttttca
      541 aaaaagaaac acctgttgaa aaaatcagtg tggaattatt gaaagaaatg cgtaatttga
      601 tgcaggaagg gatcattttt tcttttgatc aagttggttt gatcgatggt aaatatcgta
      661 tcccaatcta tgtgattgaa gattatattt ggaaattaaa aggcacccgg tatcgagaag
      721 aggaaatcat cgctacttta atctactctg tcaccgatcg acactggcaa ttggtagaag
      781 atgccgatct ttcttatctg ccattgaagt agatctattt cttatcaaaa aataaaaagg
      841 aaacaagaaa ctaggacatg attctgtctc aagtttcttg cttctttttt atttacagct
      901 cctgtcgttt atctggcaca aaaatcgaag gagtcgtttg ttggatccct aataaaacga
      961 tgatcgttac aatagctgta ctcaacccgc tggcaccatt cattacgaaa gaaaagagcc
     1021 aagaattcat cccccatagt gcatacgctc cccagaaaac cactcctgca ataaaatgcc
     1081 aaaaatatct ggaaagtgcg ccaatcaatg caccaaacac gatgcttttt ctaatttgtt
     1141 tcgtattatc gtctacatag cctttttgga catttgaagc catgagccct gctgcgcctg
     1201 caaaagtata ggcaatcggg tactcaatca acacttgaac gacacttaaa taataaactt
     1261 gtcccaacgg aaaatgtagg atgccccaaa tgaaacctgc cagcatccct gcctttgtcc
     1321 ctcttcttaa cgcaaaaaga gtcaatggaa tctgccctaa tgaaatagaa aaactgctgc
     1381 cgatctggat cggaatcaac gacaacacca tcgctaacgc tgcaacaatc gttccttcta
     1441 cccaaactct attttttccc ataaaaaaac ttcctccttt gaataaaaaa gtcacaaagg
     1501 aggaagcagc tttcgaaaat agtcaaacag ctgtttattc acaattccta cgctcgcata
     1561 accgatacag gtacgaaggg tttagaaata gattcaatct cagcccaatt ggactcccct
     1621 ttgtgagttt atttattatt tattttttgt ttcaaacaat ggactaactg gtttgttttc
     1681 atggatacgt ttgattgcat ctcccattag tccgccaaca cttacttcat cgattttgtc
     1741 gattttacga tcttctggaa gatagatcga gtctgtaaca actagacgct cgatagctga
     1801 atcttctatt ctttgtaaag cagggccgga taatactgga tgtgtacatg aagcatacac
     1861 actagttgca ccagcttctt tcaaagcatt ggcagccaat gtgatcgtac ctgctgtatc
     1921 gatcatatca tcgatcagta cacaagtttt gcctttgact tgtccaatga tattcattac
     1981 ttctgcaaca tttgcttttg gacggcgttt gtctatgatc gcaatcggag atttcaaaaa
     2041 ttccgctaat ttacgtgccc gtgtgacacc accatggtct ggtgaaacaa cgacaacatc
     2101 atcgcctttg attccttttt ctaagaaata attggcaata agtggcgcac ccatcaaatg
     2161 atcgactgga atatcaaaga aaccttggat ttggactgca tgaaggtcca aagtcagcat
     2221 tcttgttgca ccagcttttt gaatcatgtt tgctactaat ttcgctgtga ttggttcccg
     2281 tgcacgtgct ttacggtctt gacgcgcata gccataataa ggcatgacta cgttgattgt
     2341 ttttgcactt gctcgtttca gtgcgtcaat catgatcagc aactccatca agttatcatt
     2401 gactgggctg ctcgttgatt gaataacata gacatgcgat ccacgaatac tttcttcaat
     2461 gtttacttga atttctccat cactaaattg agtaacagat gattttccta actcgacgcc
     2521 gactgctgct gcaattttct ctgctaatgg gcgattggaa tttaacgcaa aaatttttaa
     2581 tcttgggtca aagtaatgtt ttgacatggg gacctccact ttctttttca caactctaga
     2641 attctaccta ataaatagta gtcatatttt ttaaataaat caagtgattt tgcaaaagtc
     2701 ttagtttaga taagggagtt tttttgcata tccttcttta ttgacttgtc ttgctctcgc
     2761 aattgccagc gcatattctg gaatattgtc tgtaattgtt gacccggcag caatcgaact
     2821 gtttttagct acctcgacag gtccgatgat attcgtgcta gaaccaataa agctgtggtc
     2881 gccgacagtc gtatgatgtt tgttctttcc atcatagttg acgaagacaa caccacatcc
     2941 gacattgatt tcttctccca gcgtagcatc accaacataa gtcaagtgac cgactttagt
     3001 tcctttgccg atttgagcat ttttcacttc cacaaagttt ccgatatgga cgttttcgcc
     3061 aatttcagct tttggtcgta aatgtgcata aggtcccaca tctgcatgtt tcttgacatg
     3121 gctgctttca atgactgagt tttcaatgac gacgtgatcc tcaatcctac tgtctacgat
     3181 ttttgaatgt gacccgatga cacaatcaga accaatgact gtatttcctt ggatttgtac
     3241 acctgcttcg atcaatgtgt ctggaccgat ttctacgcct gcatcgatgt atgtcgttgc
     3301 tgaatcgaca aagcttacgc cattgaccat atgcatctgg ttaattcgct ttctcatgat
     3361 ctcatttgct ttagctaacg cgatccgatc attgactccc attgattctt caaaatcatc
     3421 cgtttgataa gcagcaactg tatggccttc ttcttttaag atttcgatga tatctgttag
     3481 atagtattca ccttgagcat tgtttgttcc aactttgctt aacgcatcga acaaagcttc
     3541 gttgtcaaaa cagtaagttc ctgtattgat ttcttgtaca agagcttctt ctggtgttgc
     3601 atctttttgt tccacgattt tttctacgat cccaatatgg tcacggatga ttcgtccata
     3661 tcctgttggg ttttctgctt gtgccgtcaa gatcgttgca cttgcatttt ttccttggtg
     3721 atattcaaaa agattgttca gcgtttcagt agtcaataat ggtgtatcgc cactgataac
     3781 taacgttgtt cctttttttc cttccaagaa agaagcagct tgcaaaacag catgtcctgt
     3841 tcccaattgt tctgcttgta acgcatattt actgcgttct cctagttgtg ctttaacttg
     3901 ttctgctccg tgtccaacga tcgtaatcac ttcatctggt ttcgtttcac tgacccgatt
     3961 aatgatatgt tctaccatag gtttaccaga aacgggatgt aaaactttat ataattttga
     4021 cttcatgcgg gtacctttcc ctgccgccaa aataattgca taacgtgcgt ccaactgcgt
     4081 cactcctaaa tatagtttgc tgatttcagt ttatcccagt atacccattt tttcaacaaa
     4141 acaagtgatt ttcttttatc ccagcgtttt ctggataaaa aaatagatag accataacgg
     4201 gcctatctat ttggtaaccg gtgctctcct gccaactcct cggtcacaag tcacactact
     4261 cataaaacat gaaaagctgt tttttgaaat cgtttcttgt gacttagagt taaacggtag
     4321 gcttacaacc tcttcattaa acgtttttca atcagctgac cttcggtatt agctcaataa
     4381 acggcaaaat atgaggagca atttttccgt ttattgagct aatactcagg acaggacgct
     4441 gatttcacaa cctctagata aacggtgttc aaaagccagt ttctcggtat taagccaaat
     4501 ttatgaaaaa atgtggaaag ccattttcac aaaatttttc ttactactcg aaactaagcg
     4561 gctttttcac aacctcttat tctgaaaaat aatttcctgg cacaaccgta atatttttcg
     4621 tttgagtatc tacatctttg acataaagca gtgacgtata atcatcgatg gcgcgatgac
     4681 cgttgaattt tgattcggca aatacagtga tgccaacaag ttctgattcg aattcttcaa
     4741 tcaagctctt cattccgttg actgttccgc cgcctttcat gaaatcatcc actacaagga
     4801 cacgtgaacc acgtttcaaa cttctcttag acagttccat cttttcaatt ctttcagaag
     4861 atccagacac atagttgaca cttactgttg atccttctgt tatttttgag tcccggcgta
     4921 caataacgaa tggcgcattc agataatagg acacagcttg ggcaataggt acgccttttg
     4981 tggctacagt cattacagca tcgatttttt caccaaggta tttagaagca atgattcttc
     5041 caacttgacg cagcagttca ggttctccta acaaatcaga gagataaaca tatcctcctg
     5101 gaagcagacg gtcttgttct gatagacgtt ctgctagaga ttcaatatat tttttcgcct
     5161 cttcatatga aatttcaggg atgaacaata caccacctgc agcacccggg attgtttcca
     5221 aggttccata ccctcgttct ttaaatgttt tcttgatgat tcccaaatct tcactgatag
     5281 aagatttagc agattcataa cgttcggcaa agcttgttaa ggaaatcaat tcatgcgggt
     5341 gattcaataa atactgtgtc atgtcaacta gacgttcact gcgacgtact ttcacctatt
     5401 ccaactcctt ttaagaaatt ttatcgtttt acattatata agtttttctt caaaaattcg
     5461 agcattttat gaaaaaataa gaaaatagtt cggtatttat taaaataaaa agaagcctat
     5521 aaaaaatttt tcgtttttat aaggcttctt ttctttataa tttagttatt ttttcttctg
     5581 aaggcaacca atattcgttt gatgatattt acaatcagga acaaagcaat aaagactaat
     5641 gtgattgttg ctcctggagg tgtatctaga taaaacgagc tagttagtcc tgacagcatc
     5701 cctagaaatc ctacacaaac actgatagca atcacgacat tgaagctttt gcctaatcgc
     5761 ataccgattg ctgcgggaag aaccatgatt gcggatatca aaagtgcacc ggcaatcggg
     5821 atcatcacag caatagccac accagtaatc acattgaaca tcatcgacat caaatgaacg
     5881 ggtagcccat ccacgtgggc ggtatcttca tcaaaagtca gaacatacat cggccgtttg
     5941 aataaaaaga acaatatacc gatggcaacg aataagattc ctaaaaagat gacttgctcc
     6001 catgtaatgg tcacgatcga accaaacaga taggattgga tacttgttgc tgaattccct
     6061 ccgctcaaat tcatcaatac taacgctagc gccaaaccac cagccattaa aatggcaatg
     6121 gagatttctg aataggttcg gtagatcgtt cgcagatact ccaatatcac agctgctaat
     6181 acaacgacta gcatggtcgt aagtgtagga ttaaggttca aaaagaatcc taacgctacc
     6241 cctgctaaag agacatgaga aagcgtatca gccatcagcg attgtcttct gatcactaaa
     6301 aacacaccta gcatcggggc gattccggca atgaagatag cggcaagaaa cgctcttctc
     6361 atgaattcat atgaaagcaa cgccattgag aatcctcctt tctcaccagt cggatctgtc
     6421 tgtccgcata ctgtttgata tcttcatgat catgggtgat cattagaact gatttcccat
     6481 ggacatgcgc actatgtcgg agaagacgat aaaattcatt tctggattct tcatccatac
     6541 ctgttgttgg ttcatcaaga acaaataagt cagggtcagt agcaaaaata cgcgctaaac
     6601 tgattcgctg cttttgtccg ccagacaatt cacctattcg ccgatttcgc atatcccaca
     6661 tccctacagc ttctaaagca cgctgtacat gttgatgatc tttttcagag agacgcttga
     6721 accagcggcc tcttggatat cttcctgaac ggactaattc gatcaccgta ctaggaaaac
     6781 ctgcattgaa agatgcaact tgttgaggaa tataaccgat acttaatttt tttccttctt
     6841 gatttttctc agcaatcttt atcattcctt ttgaaggttt cagtaatcct aacgatgctt
     6901 tcaccaaggt tgacttggct gctccatttt ctccagttaa aatgacaaat tcacccgcat
     6961 ctacatgata agagacatct tctagtacag gttcatcatc atagtaaaag gtcaaatcag
     7021 atatttcgat ataacgcacc ttacaacctc cattctaaaa cttaattgat actttctttt
     7081 aaagctttca gatttttttc catgacagaa atataatttt cgcctgcttt gatttgttca
     7141 ttagtcaagc tttctaaagg attcaacact tctagcttca ctcctgtttc attcgacaat
     7201 gtctcagcta ccttcgaaga ggcattttct tcaaaataaa tcactttgat gttgtttttc
     7261 ttcacaaatt cttttagctc agctaagcga cttggtgtgg gttcctgatc tggtgaaacg
     7321 cccgaaatcg ctacttgttt taagtcgtat tgattcgcta aataagcaaa agcagcatgc
     7381 tgagtgacaa atgtgcggtt tgttgcatct tttagctcat cggagtattt ttgatccagt
     7441 gcttcaagct ttttgatata agcatcggca ttcttcgtaa aagtttcctt tttatctgga
     7501 tatttttcgc ctaattcttc agcaatcgtt ctgacttctt ttatagctaa taccggatcg
     7561 agccaaacat gtggatcgta ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt
     7621 tcgtctgctt cttgcgattc taaacggtcg attcctttag ctgcttcgat catcaaggtc
     7681 tgtttgctat ccactgaatc ctttaacgaa tccacaaaaa attccatgtc tgaactatta
     7741 tacacgaaaa catctgcatc ggaaatttct gctctttctt ttgcacttgg ttcgaaatca
     7801 tgcggctccg ttccagcagg gatcaaaagt ttcacgtttc cttcatcccc tacgatctct
     7861 tttgtaaaat catacatcgg ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct
     7921 gcttccttgt tcgtatttcc acatgctgca aacagtaaag cacctatcat taccgtagct
     7981 gtcaaaagat attttttcat ttatatgctc ctttcaatga acaaatcgga attttacgat
     8041 ttattaacgt gaactaatgt atcaaaataa aaaaacacag tcaatagaaa tccatcaatt
     8101 tctgatttaa gcatgtatat tcttgattaa atcgtaaaat ggatgagttg ctgctcatat
     8161 ctagccattt cactacaata aaaaactgaa gaataccatt ttaggattcc tcagtttttc
     8221 atcacctatt ttacttctta tattttattg cagggttcgt actaaataaa cttcctcaca
     8281 aaaacctttt aacccattat atactctctg cgctcttgaa taatgttgac acaaggcaaa
     8341 aacggttggt ccgcttccac tcatcaatgc agcatctgct ccgtacttga tcatacgctc
     8401 tttgatttga tggatgactg gatgccgctt tcccgttata tcttctaaac tgtttcccat
     8461 ttttttgatc atctgctgat aatcttgctc tcggatggct tcagcaagtc cgtggatgtc
     8521 ttgatgttgg atggcttgcg tatctacttc tctaaacact gtacgtgtag agacactcat
     8581 tcttggtttg accaacacaa cccagcactg tggcatagaa ggtaatactt ggatttcttc
     8641 ccctttgcca ccgacaaatg ccgtacttcc gtagacacag taaggaacat ctgtaccgac
     8701 ttccaaaccg atttctgcca gttcttctaa cgaacagccc agattccata attggttgat
     8761 ccctcttaga gcagctgcac agtctgtact tcctccgcct aatccagctg ctactggaat
     8821 attcttctta acaaagatct tgacaccggt tttgatattg tatttatttt ttagcaataa
     8881 agcagcttga taaacattgt tccgttcatc taccggtaaa aatgctttat ttgtttctac
     8941 aatgattttg tcttctgtta tttcttcaaa aaatagatga tctgccaagt ctacgctcga
     9001 catcaccatt tccaactcat gatacccatc ttgccgtcta tataatacat ccagtcctaa
     9061 atttatcttg gctggcgctt tttcaatcaa ttccatcctc tcacccattc gaaaagtcta
     9121 agtcgcaagt attctatcat agggaaaaat aaaagtatag cttatgtctg cctagtggtt
     9181 gattttgtca tcgtttcaac agctttcaca gaattttctc ttcataatcc aaacaagttc
     9241 tagtgattct tataattctg tgagcacttc ttctgccaaa ctcggaacat ttcctttttc
     9301 ttttcctatc agactgagta gctgcatggt agcaaatccg aaattacttc ctttcctata
     9361 agcaaaataa cgtccttccc aatgatcgac atattttaat ttcttttcat aagcagactg
     9421 gaaggaatag aaaaaattac cataattata tacgatatgc atgacttttt cttgaaagaa
     9481 agaaaaatct gtttctccaa cattagctaa tggagctaag cctagattgg cttccgagta
     9541 gcccattttc tcgtatacaa taaacagatt ggctaacaga tagtcagcta attcctcaga
     9601 ttctttaggt aagacccgca acaaatcata tcccgcctgc ttcttcgtca tcggctgagc
     9661 ggtgatgaag ccaacgactt tttgatcttt acggacaata ccgacaccgc tagataatac
     9721 atatgattta tcaaatcgtc ccacagagaa gtatttttct cgtgcacctt gaagccattt
     9781 ttgtgaaact tcatttacag cttgatgtaa ttcttccggc agttgatcgt accaagtaaa
     9841 atggtatcct tctttcatca aacgttgata ttcaagttgc tgactcagcc gagacaagtc
     9901 ttctgttgct agatcaacaa tccctgcttc acctactttc gtgaagtgaa aacccaattc
     9961 atgaagcatc actacgtagc tttctgatat cttataaaac gcaagctgat agcctaaaca
    10021 atctgcttgt ttcataaact gattcgttgc tgcccgcatc attttaggat tgccaactgg
    10081 atctcctaaa acaaagcatt tgtttccttt gatctgataa ccaaaaacga cttgatcttg
    10141 gtcgtcttct tggtaataaa aataactgta acctgacaag cgaagctgat ggcttgtagc
    10201 tgtgcctccg tatgtttcca ataaacggtc aaaacggtct ttttcgtact cactgccaag
    10261 cttcgtagta tcttcagcta aatattgata aagcgccact aaagctagca atgagatgcc
    10321 caaaccaata agccctgaga accacacatc atctgaagga aacaaaataa aatgatttcc
    10381 tgttattcga ccgactaaaa agcgtccgct gtgatagcct gcaattgcgt aaacgataaa
    10441 caaaaagccg aacaagctag catcaaagat tatagctccc caagaataaa cgaattttgt
    10501 tcgataaaat tcttttctgg ataaccacac agctaacaga atcaagacat ataccaatat
    10561 caactgccaa gataacgtac gtgtgattgt atttaaaata ccaaaagata gtaaagcaat
    10621 cgtaggcaga aatgcctttt tgacttttac tgaaagtgct ctagcaaggc cgagtaataa
    10681 aaagccaacc aatagattca ctgtctgatc taaaaaattg aatgagaacg gcaataatac
    10741 ttgaaatagc cgactgattg cagacagatt agtgattgtt gatagaagta ccatcatgat
    10801 ccccgcaaaa tagacagcac ttacgagcac gatatgcgca accttttgga aaaacagtcg
    10861 cggaatgtta tctaaaaatc gattgatttt gactcctgta tgtgtgatga acaatagaag
    10921 tcccgtcata aaaggtagaa cataataaaa caaccgataa tataatagcc aaactactgc
    10981 tacatcttgc cgaacagaca attgactcat tcctaagatc atcaaaacgt caaaagtccc
    11041 cattccacct gggaccatcg tcagcattcc tatcaatgta gcaatcatga acataggata
    11101 gatcgaaagc atcgaaatat ctattctcat caacttgccg atgatcaaaa ataccgcaag
    11161 ggctcccgtc cattgtccaa aggatgccag tagcagacca agcaagcctt ttggataaaa
    11221 ctcgaagaac aatgtctttc tctttagata agcaaaaaag aaaatgactg gtgctaaaaa
    11281 actaccgccg aaaagccaga tccaatagct tcgaaaaata ctttctgact gaataaaaaa
    11341 gacatcaata aatgtaagaa acgaccatac agacaaacca gtcagcataa atagtgcgac
    11401 ttttgataca gtggctaaaa ccatttttct atttgtctgc tttccataaa aactagcccg
    11461 cagacttgct cctaccacac caccaaaacc ggcgagattg ttgatcgtgt ttgttaccca
    11521 cgcagcaaga agaaactctt ttctttccat ttttggtttt ccttgttttt ctaagactct
    11581 gatcgtcacc caatcataca acaacatcgg agtgacaccc agcagtccag ccagaatcat
    11641 gagcaggatc gtcatgcggc tttgttgtcc catcctttgc caaatatctt gccaactcat
    11701 gccattagca atatttgcga cttgatttgc cacaaagact aacacggagc cgaaaaaaat
    11761 cagttttaaa atcagactat gttcctttat ccattgcagc atcaagtatc gtttattttt
    11821 cacattttcc cattcctaac ttgtatactt ttgtgtcata ccccaatgtt atcttattta
    11881 ttttaatttt tccactttag acctgttctt tgcttgcgat gatagaaaaa gaaagatatc
    11941 ctaatgctaa aggagaggat aacatgaaag taggatttat cggcacaggg aatatggcac
    12001 aagctattat tgatggttta ttaaaaaaag gaacagtctc gaaagaagag gtgttcgttt
    12061 ccagtggaca ttttgaaaat gcacaaaaat ttgctgctga aactggcgtc catgcatgca
    12121 aaagtaatca ggaagttgca tcacaatcag atatcgttct tttagccgtg aaaccaaaaa
    12181 tcattgaaaa agtattggaa gaactaagag aatatagcca gaaagtctta ttcatttcta
    12241 ttgcttcagg aaaatcatta acagatttag aaacagcgct tgaagatcct caggcctcca
    12301 tcgtacgtgt gatgccgaat gtcaatgtca gcgtaggagc aggcgtttct gcgatttgca
    12361 aaaatgaagc tgccacggag gaacagcttc agcaagctac gatgattttt gaagcaatcg
    12421 gcagcgtcta tcctttagca gaatcagatt tcagtacatt cattggcctt gctggcagct
    12481 cacccgcatt cgtttacatg ctgatcgatg cgatgggacg gactggtgta ctgaatggtt
    12541 tgtccaaaca gacagccact gagattgctg caaaagcaat tatgggttct tgtcaaaaat
    12601 tcctttcaag tgataaaaat ccatgggaac ttgtagacca agtttcttct cctggcggta
    12661 caactgttgc aggtgtagtt gccttagaag aagcaggatt catcccagca atcatcaaag
    12721 gaatcaatac gacgatcgag aaagatcttg agatgttgaa taaaaaataa tgatagcaat
    12781 gttttttcgt gccttacaaa aaaagaagag ctgtggcttt tgccgcggct cttcttttgt
    12841 attttttatt atttagcata atcagttgca cgagtttccc gaataacgat tactttgatg
    12901 tgtccaggat aatccagttc ttcttcgatt cgtttacgaa tatcacgtac taaacgaaca
    12961 gcgtctaaat ccgagatttc ttctggcttg accattaccc gaacttcgcg tccagcttga
    13021 actgcgtaac ttgattctac accggcgaag ctgttcgaaa tattttccag acttcttaaa
    13081 cgacggatgt agttttctaa tgattcactt cgcgcaccag gtcttgctgc tgagagcgca
    13141 tctgctgcag ctacaaggac agagataaca gaagtagctt ccacatctcc atgatgagaa
    13201 gcgatcgcgt tgattactac tgggttttct ttgtacttgg cagctaattc cgcaccgatc
    13261 tcaacgtgag atccttcaat ctcatggtca agtgctttcc caatatcatg gagcaacccg
    13321 gcacgttttg ctaattggat atcttcgcca agctctgctg ctaagatacc agctaatttt
    13381 gcaacctcga ccgaatgctt caagacgttt tgtccataac tagttctgaa tcgcagacgt
    13441 ccgatgatct tgattagatc tggatgaagt gtatgagcac cgacttcaaa cgctgcctct
    13501 tccccgtatt cgcgaatacg ttcatccatt tctttacgag atttctcgac catttcttcg
    13561 atacgcgctg gatggattcg tccatcttgt atcagttttt ccaaggtcat ccgtgcgatt
    13621 tctcttcgga ttggatcgaa tccggaaaga acaactgctt ccggtgtatc atcgatgatt
    13681 aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg tccaatgata
    13741 cgacctttca tttcatcatt cggaagggta acgactgaaa cagtcgtttc tgaaacttga
    13801 tctgctgcgc agcgttgaat cgctaatgat aataagtttt tcgctttgcg atctgcttct
    13861 tctttggcac gctgttcaga ttcttttacc attagagtta actcatgatt cagctcttct
    13921 tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg ataaaccagc aattttttct
    13981 agttcttttt gttgatcttc aatcattttt tcaacgtctt tttctcgctc ttcaattaat
    14041 tgctgttttg aactaagtgc ttcttcttta ctttcaagtg agttctcgcg tttttctaac
    14101 gagtcatctt tacgattcag cgtttgttcg cgttgaatca aacgattctc ttgagatttt
    14161 aattctaact tactttctct cagctcactt tcaacttcag aacggtattt ctgattttct
    14221 tccttagctt ctaacaatgc ttcttttttt agagtttctg cttctttttt tgcactatcg
    14281 atgattccag cagctgatga ttgtgcacca gctatttctt tctcgtgacg tgactttgcg
    14341 accataaacc ctaaaccaag accgacaatt aaaccgatga tagcgaggag aatgttgaat
    14401 accataaaat ccacctccat actatctttt tcatttttat catctataca gcttctatga
    14461 taaattattc taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac
    14521 aactttattc taatgtttgt accccgtagt gtcaacttta aataaacaaa gaaacggctt
    14581 tctacttatt cattaaacga tggtttttaa aataaatgac actagttt
//
LOCUS       DAXWLH010000064        14614 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXWLH010000064 DAXWLH010000000
VERSION     DAXWLH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.064"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="ACGE36_001465"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="ACGE36_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFF8383863.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..3118
                     /gene="iadA"
                     /locus_tag="ACGE36_001466"
     CDS             1934..3118
                     /gene="iadA"
                     /locus_tag="ACGE36_001466"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFF8383864.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3229..3432
                     /locus_tag="ACGE36_001467"
     CDS             3229..3432
                     /locus_tag="ACGE36_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8383865.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3533..4195)
                     /locus_tag="ACGE36_001468"
     CDS             complement(3533..4195)
                     /locus_tag="ACGE36_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383866.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4208..5710)
                     /locus_tag="ACGE36_001469"
     CDS             complement(4208..5710)
                     /locus_tag="ACGE36_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383867.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6258..7937
                     /locus_tag="ACGE36_001470"
     CDS             6258..7937
                     /locus_tag="ACGE36_001470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8383868.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8064..8543
                     /locus_tag="ACGE36_001471"
     CDS             8064..8543
                     /locus_tag="ACGE36_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFF8383869.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8762..9442
                     /locus_tag="ACGE36_001472"
     CDS             8762..9442
                     /locus_tag="ACGE36_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8383870.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9450..10781
                     /locus_tag="ACGE36_001473"
     CDS             9450..10781
                     /locus_tag="ACGE36_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFF8383871.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10796..11758
                     /locus_tag="ACGE36_001474"
     CDS             10796..11758
                     /locus_tag="ACGE36_001474"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFF8383872.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11869..12207
                     /locus_tag="ACGE36_001475"
     CDS             11869..12207
                     /locus_tag="ACGE36_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFF8383873.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12286..12993
                     /locus_tag="ACGE36_001476"
     CDS             12286..12993
                     /locus_tag="ACGE36_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFF8383874.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13096..14577)
                     /locus_tag="ACGE36_001477"
     CDS             complement(13096..14577)
                     /locus_tag="ACGE36_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383875.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4640 a         2525 c         3145 g         4304 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3121 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3181 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3241 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3301 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3361 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3421 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3481 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3541 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3601 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3661 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3721 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3781 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3841 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     3901 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     3961 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4021 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4081 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4141 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4201 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4261 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4321 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4381 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4441 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4501 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4561 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4621 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4681 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4741 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4801 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4861 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     4921 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     4981 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5041 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5101 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5161 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5221 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5281 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5341 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5401 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5461 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5521 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5581 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5641 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5701 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5761 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5821 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     5881 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     5941 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6001 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6061 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6121 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6181 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6241 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6301 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6361 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6421 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6481 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6541 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6601 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6661 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6721 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6781 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6841 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     6901 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     6961 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7021 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7081 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7141 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7201 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7261 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7321 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7381 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7441 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7501 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7561 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7621 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7681 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7741 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7801 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7861 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     7921 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     7981 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8041 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8101 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8161 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8221 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8281 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8341 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8401 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8461 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8521 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8581 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8641 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8701 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8761 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8821 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     8881 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     8941 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9001 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9061 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9121 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9181 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9241 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9301 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9361 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9421 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9481 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9541 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9601 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9661 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9721 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9781 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9841 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     9901 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     9961 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10021 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10081 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10141 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10201 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10261 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10321 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10381 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10441 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10501 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10561 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10621 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10681 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10741 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10801 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10861 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    10921 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    10981 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11041 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11101 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11161 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11221 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11281 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11341 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11401 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11461 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11521 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11581 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11641 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11701 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11761 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11821 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    11881 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    11941 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12001 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12061 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12121 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12181 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12241 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12301 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12361 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12421 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12481 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12541 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12601 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12661 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12721 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12781 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12841 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    12901 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    12961 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13021 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
    13081 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    13141 ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    13201 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    13261 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    13321 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    13381 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    13441 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    13501 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    13561 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    13621 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    13681 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    13741 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    13801 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    13861 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    13921 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    13981 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    14041 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    14101 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    14161 gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    14221 ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa
    14281 catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac
    14341 agctaacttt ttagtttgat cggtttcttt ggtttggatc tctcctttat gattagaata
    14401 aataaattca tcaatcaata aaaattgctt gagtgataca tttatttttc ggatcagttc
    14461 tttgattgtc cgttttggaa tatgtaagtc attggataat tcttgtaaag aacaattatt
    14521 tttttgaatg accatttgta gaacaagtaa ctcttttttt cttgcttcaa ttagcataaa
    14581 tacctccttt ttattctgtt tttgtatata gtta
//
LOCUS       DAXWLH010000065        14523 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXWLH010000065 DAXWLH010000000
VERSION     DAXWLH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.065"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..941
                     /locus_tag="ACGE36_001478"
     CDS             <1..941
                     /locus_tag="ACGE36_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383876.1"
                     /translation="NELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1162..2925
                     /locus_tag="ACGE36_001479"
     CDS             1162..2925
                     /locus_tag="ACGE36_001479"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFF8383877.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3009..3467
                     /locus_tag="ACGE36_001480"
     CDS             3009..3467
                     /locus_tag="ACGE36_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFF8383878.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3576..4259
                     /locus_tag="ACGE36_001481"
     CDS             3576..4259
                     /locus_tag="ACGE36_001481"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8383879.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4406..5020
                     /locus_tag="ACGE36_001482"
     CDS             4406..5020
                     /locus_tag="ACGE36_001482"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFF8383880.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5036..5242
                     /locus_tag="ACGE36_001483"
     CDS             5036..5242
                     /locus_tag="ACGE36_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383881.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      5467..5633
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            5781..6338
                     /locus_tag="ACGE36_001484"
                     /pseudo
     CDS             5781..6338
                     /locus_tag="ACGE36_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6396..8435
                     /locus_tag="ACGE36_001485"
     CDS             6396..8435
                     /locus_tag="ACGE36_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8383882.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLYAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(8544..9695)
                     /locus_tag="ACGE36_001486"
     CDS             complement(8544..9695)
                     /locus_tag="ACGE36_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8383883.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9849..10379)
                     /locus_tag="ACGE36_001487"
     CDS             complement(9849..10379)
                     /locus_tag="ACGE36_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8383884.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(10478..10828)
                     /locus_tag="ACGE36_001488"
     CDS             complement(10478..10828)
                     /locus_tag="ACGE36_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFF8383885.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(10985..12514)
                     /gene="ahpF"
                     /locus_tag="ACGE36_001489"
     CDS             complement(10985..12514)
                     /gene="ahpF"
                     /locus_tag="ACGE36_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFF8383886.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(12524..13087)
                     /gene="ahpC"
                     /locus_tag="ACGE36_001490"
     CDS             complement(12524..13087)
                     /gene="ahpC"
                     /locus_tag="ACGE36_001490"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFF8383887.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(13231..13893)
                     /locus_tag="ACGE36_001491"
     CDS             complement(13231..13893)
                     /locus_tag="ACGE36_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8383888.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            14077..>14523
                     /locus_tag="ACGE36_001492"
     CDS             14077..>14523
                     /locus_tag="ACGE36_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFF8383889.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYR"
BASE COUNT         5002 a         2577 c         2776 g         4168 t
ORIGIN      
        1 gtaacgagtt gattgattat gtagagactc atttttttaa aagaagatat tcggaagatg
       61 aaaagtcctt tatcatacga ggaatagaga tcgcaatcgg aagaaaggca tttccccttg
      121 gactgaaaga agaagataag aaaagcgcag aaacgaagcc gttatttcaa ttagtctgtc
      181 aaggattgga agagcaaaaa ggaattctag acttacaaga agaagatgtc tattatattt
      241 tttctttgtt caacagtaga aattatacca atgagaacat ggagctgtta agaaaagatt
      301 tcgaagttgt ttataaaaat tttgttctca atgacctatc actgaatgaa ttgattttac
      361 agattattag tatcgctgaa atcgacgatg cagaaaattt tattttcaag caatcctttt
      421 tacaatttgt ccgtacgttg tgggcagatg gacaagtatt tttaccggaa aggatctatc
      481 ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt agaaatattg gaagagtgga
      541 aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa ttcgtcaaaa
      601 gtctttcttt gatcaacagt gaacaagcgg ggagacagga aatagaaatt tttgttgttt
      661 cagaaagttc agtcaaacaa tttttttatc gaacccaatt tcatttgtat gcggaagaag
      721 gcatcgctga aataaatcct attttatttc gttcattaga agaattgcct gatgaatgtc
      781 tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat ggattagaaa
      841 ctgaaaagac aaagatctat ccaatctcat taaaaggtgc aaacatccat ttgtatcata
      901 ttgctcagga acttcaccta ttaaaaaaac atatttcttg atgagacctt aattaggaga
      961 ccttctacaa agctttttaa ttgtctgtag aaggttttta tttgagtcct gattcgtgcg
     1021 aagatctact tatataacaa gctttctctg aaattttttt aaaatgctat tgacttagag
     1081 tcaactctaa gtagtagaat agataaagtg taacaaatct attttcatta aaagaagata
     1141 gtacggagga gagaatgtat tatgagtaca gtattacatg tattggatgc aatcaatagc
     1201 cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac agttggcaga agagatgcgc
     1261 caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg ttggtccgaa tctaggtgta
     1321 gtagaattaa cgattgcgtt tcattatgta tttgattcac caaaggataa aatcgtttgg
     1381 gatgtttccc atcagtctta tggtcataag cttttaactg gtcgaaaaaa tggttttgaa
     1441 aaaggacatt accgtgaagt gagcggatat tccaaccaac atgaaagtaa acatgactat
     1501 tttacagtgg ggcacacatc tacatctatc agcttagcaa caggtttagc taaagcgaga
     1561 gatctgaaga aagaatcagg aaatgtggta gcatttatcg gtgacggctc cctaagtggt
     1621 ggcttagctt ttgaaggatt gaacaatggt ggtgttttac aatccaatct aatcgtatta
     1681 gtaaacgata atgagatgtc tattgatgag aatcatggca gtttatataa aaacttagct
     1741 gagcttcgtc aaaccaacgg taaaagcagc aataacctat ttaaagcaat gggatttgat
     1801 tatcgttacg tggataatgg aaacgatctg gaaacggtga tccgtttatt tgaagaagta
     1861 aaagatattg atcatccgat tgtacttcac gtccatacgg aaaaaggtcg cggttatcag
     1921 ccggcaatcg ataataaaat gaaatatcat tggcatgtac catttgatct gtccaccggg
     1981 gaggcaaagc aagtaacttc tggagaaaca tttaatatga tcatcatgga ttatttggat
     2041 aagaaaatgc aaacaagtac agctcctatt gccgcagtca atgcagcgat accaggcgcc
     2101 tttggactga aaacattcca agaaaaacat ccagatcatt attttgacgt ggggatcgct
     2161 gaacagcact ctatcacttt cgcttctgct ttggcttctc agggagtacg tccagtcgtc
     2221 ttccaatcaa gtactttctt gcaacgggca tatgatcaat tatcacatga tttagcgatc
     2281 aacgagaatc cagtgattat tttagtcggt ggcggaacga tctcagctag tgaccgaaca
     2341 caccaaggaa catttgacat tccttactta tcaagtatcc cgaacatcaa atatcttgct
     2401 ccagcaacaa aagaggatat gatctctatg ctagattggg cgttcgaaca aacagcgcag
     2461 cctgtagcaa tccgaattcc tacacatggt gtagaacatg gtacagctat cgcaaatgat
     2521 tatagtattc cagcctatca aacagtaaat aatggaggaa aagtagcaat tttagcttta
     2581 ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt ttagaaaaaa aggaatcgaa
     2641 gcaacgttga tcaatccagg aacgatcaca gaaaatgacg tagaaacatt agataaatta
     2701 aaagaaacac acgaattagt catcacatta gaagatggta gtttgtcagg aggatttggc
     2761 gaaaatatca gtcgttatta tggtgattct tcaatgaacg tattaaattt tggtgcagag
     2821 aaagaattca cggatagcgt tcctgtaggg cagctttatg aacgttatca tttaacacca
     2881 aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc aataattcat tatgctcacc
     2941 atccagataa ttgtttatac tagtatctgg atggtttttt cattataaaa agcaaagggg
     3001 tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa aaatatccta
     3061 gttatcaatc agaaaaatta ggtttctttt catttgggga tacagaaaaa atggcagacg
     3121 agttgagcga actagttgta aaaaagataa agactggtac atcaagtggt tacgatctgt
     3181 ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa
     3241 aaaatgaacc agtttgtatc atcaaaaata taaatgtgac catcttgcct tttagtgaag
     3301 tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg gacattagct tattggcgga
     3361 aagtccatgc tgagtttttt aaacatgctt ataaagaagc gttgaacata gaatttacag
     3421 aagagaagct ggtcgtatac gaagaatttg aagtaatttt cttttagtaa gaaaggtctg
     3481 ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag ggttacattt ttatatgaag
     3541 ctgtattttt atataacatt actggaggag atattgtgga tacactggaa caaatcaaag
     3601 gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc gctacacagt tcttttatta
     3661 tggggagaat ggctttggct gcaaaagaag gcggagcagt tggtattcga gcaaatacga
     3721 cagaagatat caatgaaatc aagcgaatcg ttgatttacc ggtaataggg attatcaaaa
     3781 gagattacga tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt
     3841 tggagacgca agcagaaatc attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa
     3901 aaacaactgc tgaacttgtc caaatgattc acgaacacgg tcggttagct atggcagata
     3961 tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt ttgtctacga
     4021 ctttagcagg ctatacagaa tattcacgaa agacgcagac acctgatttt gatttattga
     4081 atcaaatcat agaacaagtc gatatcccag taattatgga aggacatact gactctcctg
     4141 aacaagtaga gaaagcatat gaattaggtg ctttttcagt cgtagttgga tctattatca
     4201 cccgtccgca attgatcact aaaagatatg tcgaagccgc aagtaaagca aataactaaa
     4261 aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga
     4321 ttctaagaag atgaaaaaag cattactttt cattttgtct tcaagatctg ctttttaacg
     4381 aattttatag aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat tgacagaacg
     4441 tgaaaattac aagttgctga ttggttcagt catccctaga ccagtagcag tagtaacgac
     4501 taaatcagca aaagatatag tgaatatcgc acccttcagt tacttcaata tcgtcagttc
     4561 gaatcgaccc atcttatcct tggcgatcca gagaaaacaa gatgcagtaa aagacacagc
     4621 aaagaatatt cttgagacaa gagaagctgt tatccatatc cttgatgagg acaacgtaga
     4681 agaagtaaat aaaacagcag ccaatctgcc taatgaggaa agcgaactgt acctgactga
     4741 cttgacttta actgattcag taatcatttc agtaccagca ttgacagaag caaaagttcg
     4801 ttttgaaaca tccttatatg aacatgtcga gatacggaat aaaaataaag taacagctga
     4861 tttatttttg ctagaagtac agaaatatca tttaaatgaa acagtctatg atgataagac
     4921 aggaaggatc gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa atgattatgc
     4981 agcaatagga gaaattttca cgattgcacg tcccgaataa aaaggagcac tagttatgtt
     5041 aaaaataagc tataaaccat cgacagattc aaaagaaatg aaaaaagaat atgaaacagt
     5101 gaatgatttt ctgcaagggc agtacttaga agttccgcca ttgcaagacc attttgtcgt
     5161 tactactgtt actttagatg gtaaggaaat agaaatgcca gatcaaacga ttagcggatt
     5221 gtttaattac tttaacaaat aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac
     5281 acataaaact aaaaaattat caaagaatcc tggtgcaact gaaagaattt gcaacgggat
     5341 tctttcaact tatttgttat ttttttctaa aaaatattaa agattgttaa atttcactat
     5401 ttcatttcaa attccattgc ctaatcatta ggaaaagtct atcatgaact cgtaaataga
     5461 atatagtaat aaccttgtta ggtgaggctc ctatacaaac ataggctgtt gcccagaaac
     5521 gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt aaggcttttt ccaatagcta
     5581 agaatgtttt ctttacgttg tatagtgcta aaactcggac gatgagatga tgctaggtca
     5641 gaattcttgt tcgaaaaggt ttctgacctt ttctttatat ttttgtgact gacttatttg
     5701 atgagacaaa ctcgctcctc acctgacaag tggattgctg tgtgaggagg agatgcggtg
     5761 aaaaaagatc gattggtgga ggtcgctcgg attcacttct ggttgcacaa tacaaccaga
     5821 aaacacaaca agcaaaactg atctctatca tgagagattc atatgtcgat attccaggtt
     5881 acggaatgaa taagataaat gcagcttact cttatggggg agttgattta ttaaatcaaa
     5941 cattaaagga aaatttcaaa tttgaagctc cctattatgc aagtatcaca tttcaagatt
     6001 ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc
     6061 tagatttaga tggcgtatat ataaacaaag gagagcaagt gatggatggt aatactttac
     6121 tccagtatgc tcgatttcgg gaagacaaag aaggagactt cgggaggatc agaaggcagc
     6181 agcaagtgat caaagctatc tctcagcagc tgaaagacgt aacttcaata ttcaagctac
     6241 ccaaagcggt tgggaaatta cttggtagta tccaaacgaa ccttccagaa agtgtgcttc
     6301 tggattgtgg tatggatttc ctcaaagaca tatgttagaa taaacaggta taaatagtga
     6361 aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac aagcacggca
     6421 aaaatcgaac aggcgcttat attgccggcg cagtgatctt aatagcagct gcgggtggcg
     6481 gttattttta ctaccagcac tatcaagaaa cccaagcagt agaagctgga gaaaagacag
     6541 ttgagcaatt tgtccaagct ttgaacaagg gagattataa caaagctgca gaaatgacat
     6601 caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga gatcttagat aaataccaga
     6661 atatatacgg tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa
     6721 aagacgattc tacttacagt ttttcatata aagcaaagat gaatacctca ttaggtgaat
     6781 tgaaagatct ttcttataaa ggaacattag acagaaatga tggtcaaacc acgatcaact
     6841 ggcaacccaa cttggttttt ccagaaatgg aaggaaatga caaagtaagc ctgaccacac
     6901 aagaagcagc aagagggaat attatagatc gaaatggtga accattagca acaacaggca
     6961 aactaaaaca attaggagtc gttccaagta aacttggaga tggaggcgaa aaaacagcca
     7021 atatcaaagc gattgcttcc tccttcgact taacagaaga tgctatcaat caggcgattt
     7081 cacaaagctg ggtacaaccc gattactttg tcccattgaa aatcattgat ggagcaacgc
     7141 cagaacttcc agctggagct accattcaag aagtagacgg cagatattat cctttgggtg
     7201 aagcagctgc tcagctgatt ggttacgtgg gagatatcac agcagaagat attgataaga
     7261 atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg gcttttgata
     7321 aggatcttcg tgggacaaca ggtgggaaat taagcattac agatgcagac ggtgtagaga
     7381 aaaaagttct gatcgaacat gaagttcaaa atggcaaaga tatcaaattg acaatcgatg
     7441 cgaaggcaca aaaaatagct tttgacagtc taggaggaaa agctgcatca actgttgcga
     7501 caacgccaaa aaccggtgat cttcttgcgc ttgctagctc tccaagctat gatccaaaca
     7561 aaatgacaaa cgggatctca caagaagatt acaaagctta tgaagaaaat cctgaacatc
     7621 cattcatcag ccgatttgcg acaggttatg ctcctggatc tacgtttaaa atgattacag
     7681 cagcaatcgg tctcgacaac ggcactatcg atccaaatga agtgttgacg atcaacgggc
     7741 ttaaatggca aaaagacagt tcttggggat cttttaaaac aacgcgtgtc agtagtgatg
     7801 tatcacaagt agacttaaaa actgctttga tctattccga taatatatat gcggcacaag
     7861 aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt agataaattc atttttggtg
     7921 aagaccttga tttgccaatt agtatgaatc cagcacaaat ttctaatgaa gatagcttta
     7981 actcagatat cttgctagct gatactggat atggacaggg cgaacttcta attactccta
     8041 tccagcaagc agcaatgtat tctgttttta ccaacaatgg cacacttgtc tatcctaaat
     8101 tgattgcaga taaagagaca aaagataaga agaatgtcat cagcgaaaca gcagtacaaa
     8161 cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt
     8221 ctgctttagg gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg
     8281 atgtaaaggg gcaagagaac agtttcttgt atgctttcaa tcttgataac caaggttata
     8341 tgatggtcag catgttggaa aataaagaag atgatgattc agcaaccaaa cgagcatcgg
     8401 aactattgca atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa
     8461 aggactgtgg cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct
     8521 ttcacaactt ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga
     8581 acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt
     8641 ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc
     8701 gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc aatctgagca
     8761 ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat agaaataaag
     8821 agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac aaaaaatact
     8881 ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc tttaaccttt
     8941 gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag cccaacaaga
     9001 tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat agaagaattt
     9061 gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac taataagaaa
     9121 ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa
     9181 gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa aatgtcatct
     9241 accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt tagttctttt
     9301 aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct
     9361 tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac cgggaagatg
     9421 atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag agataaatca
     9481 ctttccagac cagctaaaaa cattaataag atcacaccaa tctccgaaaa gtcatggact
     9541 agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc aacaagtaat
     9601 tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat cgttgttgaa
     9661 atcaatatca ggcataaaat accaataaat tccattctca tcattccttt gtataaaata
     9721 tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac tcacaaaaaa
     9781 accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat tttaataact
     9841 aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat ctcctggatg
     9901 cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact tttgaatgga
     9961 ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac actccaactt
    10021 ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat aacagctaat
    10081 ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa aagcatagac gatcagagct
    10141 aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt tttcctttta
    10201 aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc ggtgatcaga
    10261 taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc agaaataata
    10321 taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata
    10381 taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa attaaatgtt
    10441 acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg atcttttcca
    10501 tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt tctttcatca
    10561 agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc aaggaacgtg
    10621 aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt
    10681 gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca tctacttcct
    10741 cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg cttgcaaaac
    10801 ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct ttcttttttt caaattattt
    10861 actacttata gtatataaga aaaagagaag aaaaagcgat tattcaaaaa aacaaaaagg
    10921 attgtgacaa aattcttgtc accatccttt tttccgctct ctgacttttt acaatcttca
    10981 tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct cctgaaccca
    11041 ttgaaacaat gatttgtttg tatggactat ctgtacaatc accagctgcg aaaacgccgg
    11101 ggatattcgt tgcgccattt ttctcggtca ctatttcatt tcttggattc atttcgatcg
    11161 ttccatctag ccaatccgta ttagggagta agccgactag aatgaatact cctgaaacag
    11221 ctagtgtatg catctccatc gtacggcgat cgatatagga gagagattct acctgattcg
    11281 taccgctgat ttctttcgtc gctgcatgtt tgataacagt gacattcgca agagagacta
    11341 gtttatcctg taagatttga tctgctttta attctggtaa gaattctaga acatatacat
    11401 gttttgctaa cccagccaaa tcgattgctg cttcaacacc agaattacca ccaccgataa
    11461 cagcaatctc ttttccttta aataaagggc cgtcacagtg tgggcaataa gcaattcctt
    11521 tatttttaaa ctctttttcg cctggtacat tgatagaacg ccagtgcgca cctgtcgcga
    11581 tgatggcggt ttttgcttta agtgatatac cgttagctaa gccaatatta ataaaatgtt
    11641 gctctttatt taaggaaacg gcctgttgat tcatcataat gtcgataggg taactacgca
    11701 catgactttc tacttgctgc atcaattttg gaccttctgt gtaagaggta ccaatcatat
    11761 tttcaatccc tagtgtttca acgacttgtc cgccgaaaga atcagcgaca agtcctgtac
    11821 ggattccttt acgtgctgca tagatagctg cactactagc tgctggtcca ccgccaatca
    11881 ctaatacatc ataaggttct ttttcaaaca actcttcttc aatcaaaggg ccagtgattt
    11941 tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca tttaaaaaca
    12001 cagttggcac tgccatgatt tttttatcat cgatttcttt ttggaacatc ccaccttcaa
    12061 tcatcgtatg agtaatattt ggatttaata cagctaatat attcaatgcc tgtacaacgt
    12121 cggggcaatt atgacaagtc aagctaacat aactgtcaaa atgcaatgtt tgtgttattt
    12181 ttttgatttt aagactcaaa gattcactga ttttcggtgc ccgtccacta atttgtaaca
    12241 tagccagtaa aaaagaggaa aattcatgac cgagggggat accagcaaaa acgattccac
    12301 tttctttgcc aactggattg atagaaaaac taggtgtgcg catcgaatct cttttttccc
    12361 aagagatttt aggagacatt tctgctattt ccttcgtaaa ttcagctagt ttttgactat
    12421 tttcactatt atccaaacta acagaaaaaa caataggaga ttcaatcagt gctaaatatt
    12481 gttcgagatt ttgtttagtt tctttgtcta acatatccac cacctaaatc tttccaacaa
    12541 gatcaaaact tggtttcaat gtttcgccac tttctttcca ttttgccgga caaacttcac
    12601 ctgggtgggt acgtacatat tgagcagcgc gaatcttatc aatcagtgca ctagcgtcac
    12661 gaccgattcc atctgcattg atttccatag cttgaacgat tccgtctgga tcaataataa
    12721 atgttcctcg ctgagctaat ccttgctctt catccaaaac atcaaaaata cgagaaatct
    12781 gatgagacgg atcaccgatc atcacgtatt cgattttacc gatagcatct gatgtatcat
    12841 gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga aaagacttca acacctaaag
    12901 atttcaatgt actgtattgt tcttgtaagt cttctaattc agtaggacaa acaaaagtaa
    12961 aatcagctgg atagaagcaa agaatactcc agttccccat aagatctttt tcactgactt
    13021 ctaaaaattc tccttgatga taagcatgtg cattaaattc ttcgattttt ttcccaatca
    13081 atgacatagt aaaacctcca ataataatta tttatttata attattgtaa tttagttgga
    13141 gttgcaaagt caatggatat tttgttattt aataaagatt actcaaaaac gaaggttaat
    13201 tataaaaata gaagaggtcg caagaaaatg ctactgctgg aaatactcgg gataacgttg
    13261 agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc aatcccgttt gaaatcgagc
    13321 ttcttctggt tttttatcaa ctaaagcctt gagtaaatca taatgttgtt ccatgatcgt
    13381 ttcccatgga agcccctgaa tggtcactcg taaccagcgg aaacgttcta aatgggtatt
    13441 atggtcatct aaccagttcc aaacttcttt tcttcctgca atttcgaaac aagcttcatg
    13501 aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt attttgactg ctttttcttg
    13561 ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg tccaatttgg aacaacattc
    13621 aatcatgatt tgctcttcca actgttctcg gacaaagcga gcattttctg cagaaacaag
    13681 attaattttt gaaacatacg taccactttg aggtatcgta tacacgagtt cttgctgacg
    13741 tagttgaatc aatgcttcgc gaatgggagt ccggccgata gcaagatgtt cctccagtgc
    13801 cttttcagat attttccttc caggttctaa gtcagcatag ataatttgtt tacgaatcgc
    13861 ttgataagct tgagattgca agctgctttt catctttatt cctccaaaat catttctatg
    13921 aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta actagtaaat
    13981 ttgtataaat agtaaaattt tggttgacat agcgattttt attgcataaa attcacttga
    14041 atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa caactggagg
    14101 aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga ccgtgcatca
    14161 ttaagagaaa atggacaaaa aaatcctgtc tggatccatt tcggtggcgg aaatctttat
    14221 cgaggatttc atgcagaaat cgctcaacgt ttagcggatc taggcgaatt gtccactggt
    14281 gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa gggcttacca agattttgat
    14341 gatttgattc ttgaagtggt catgccaagc agtggagagt tcgtcaaacg tatattagct
    14401 gcgacagctg acagtctgta ttgtcatcca gattgttctg aacactttga taagatgatt
    14461 cgacactttg aaaatcctag tttacaatta gttacttttg ctattactga aaaaggttat
    14521 cga
//
LOCUS       DAXWLH010000066        14424 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXWLH010000066 DAXWLH010000000
VERSION     DAXWLH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.066"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            115..696
                     /locus_tag="ACGE36_001493"
                     /pseudo
     CDS             115..696
                     /locus_tag="ACGE36_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            700..1341
                     /locus_tag="ACGE36_001494"
     CDS             700..1341
                     /locus_tag="ACGE36_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383890.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1338..2255
                     /locus_tag="ACGE36_001495"
     CDS             1338..2255
                     /locus_tag="ACGE36_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8383891.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2252..2923
                     /locus_tag="ACGE36_001496"
     CDS             2252..2923
                     /locus_tag="ACGE36_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383892.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3464..3952)
                     /locus_tag="ACGE36_001497"
     CDS             complement(3464..3952)
                     /locus_tag="ACGE36_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFF8383893.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4153..4419)
                     /locus_tag="ACGE36_001498"
     CDS             complement(4153..4419)
                     /locus_tag="ACGE36_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8383894.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4419..4682)
                     /locus_tag="ACGE36_001499"
     CDS             complement(4419..4682)
                     /locus_tag="ACGE36_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8383895.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5203..5343
                     /locus_tag="ACGE36_001500"
                     /pseudo
     CDS             5203..5343
                     /locus_tag="ACGE36_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5485..7308)
                     /locus_tag="ACGE36_001501"
     CDS             complement(5485..7308)
                     /locus_tag="ACGE36_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8383896.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7906..10611)
                     /locus_tag="ACGE36_001502"
     CDS             complement(7906..10611)
                     /locus_tag="ACGE36_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFF8383897.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10917..11864)
                     /locus_tag="ACGE36_001503"
     CDS             complement(10917..11864)
                     /locus_tag="ACGE36_001503"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFF8383898.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            12054..12323
                     /gene="rpsN"
                     /locus_tag="ACGE36_001504"
     CDS             12054..12323
                     /gene="rpsN"
                     /locus_tag="ACGE36_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8383899.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12499..12861)
                     /locus_tag="ACGE36_001505"
     CDS             complement(12499..12861)
                     /locus_tag="ACGE36_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8383900.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12872..13993)
                     /gene="alr"
                     /locus_tag="ACGE36_001506"
     CDS             complement(12872..13993)
                     /gene="alr"
                     /locus_tag="ACGE36_001506"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8383901.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(14009..14359)
                     /gene="acpS"
                     /locus_tag="ACGE36_001507"
     CDS             complement(14009..14359)
                     /gene="acpS"
                     /locus_tag="ACGE36_001507"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFF8383902.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         4075 a         3073 c         2709 g         4567 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca
      181 gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc
      241 ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc
      301 acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta
      361 gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg
      421 ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt
      481 tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg
      541 aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca
      601 ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca
      661 gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag
      721 cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg
      781 atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct
      841 gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg
      901 gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt
      961 tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat
     1021 tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa
     1081 ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt
     1141 gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt
     1201 ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta
     1261 gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt
     1321 agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc
     1381 ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt
     1441 cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg
     1501 ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca
     1561 accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag
     1621 atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg
     1681 tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat ggttttgaaa
     1741 atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca
     1801 gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc
     1861 gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat
     1921 tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc
     1981 ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc
     2041 gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata
     2101 cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc
     2161 aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt
     2221 tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt
     2281 tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg
     2341 tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg
     2401 gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat
     2461 ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt
     2521 caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta
     2581 taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac
     2641 tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg
     2701 cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc
     2761 tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat
     2821 tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat
     2881 tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca
     2941 aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc
     3001 aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta
     3061 tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta
     3121 agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga
     3181 attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc
     3241 ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg
     3301 ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat
     3361 acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc
     3421 tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt
     3481 cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt
     3541 gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg
     3601 cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt
     3661 gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac
     3721 tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga
     3781 accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt
     3841 ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg
     3901 ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc
     3961 ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca
     4021 aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt
     4081 tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg
     4141 gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc
     4201 ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc
     4261 atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt
     4321 ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac
     4381 tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata
     4441 tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta
     4501 cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat
     4561 acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc
     4621 tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc
     4681 attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa
     4741 atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc
     4801 cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc
     4861 caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg
     4921 tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat
     4981 atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt
     5041 ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg
     5101 ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg
     5161 gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa
     5221 gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca
     5281 ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc
     5341 taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg
     5401 cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt
     5461 ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac
     5521 gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat
     5581 attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg
     5641 tttcgctgca tttttactta cgatatctac ataacggata attggatttt caatcgagcg
     5701 ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc
     5761 gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc
     5821 accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa
     5881 tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg
     5941 ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat
     6001 aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc
     6061 atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc
     6121 ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat
     6181 agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga
     6241 acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata
     6301 catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa
     6361 accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa
     6421 aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg
     6481 aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa
     6541 tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac
     6601 agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa
     6661 aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg
     6721 cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat
     6781 aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag
     6841 catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg
     6901 caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta
     6961 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
     7021 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
     7081 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
     7141 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
     7201 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
     7261 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
     7321 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
     7381 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
     7441 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
     7501 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
     7561 cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct
     7621 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
     7681 catctgcggt aataagctca accaaattaa aaacataaga agctgttttt atttggtcgt
     7741 tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag
     7801 ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt
     7861 ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg
     7921 gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc
     7981 cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc
     8041 atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg
     8101 caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc
     8161 tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa
     8221 taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga
     8281 cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac
     8341 gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc
     8401 tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact
     8461 tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga
     8521 accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa
     8581 gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc
     8641 ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca
     8701 gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc
     8761 atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat
     8821 aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc
     8881 tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc
     8941 atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac
     9001 tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc
     9061 tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt
     9121 gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac
     9181 aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc
     9241 tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag
     9301 ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa
     9361 ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa
     9421 tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct
     9481 tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac
     9541 atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt
     9601 ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca agatatcttg
     9661 tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc
     9721 ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc
     9781 tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt
     9841 gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa
     9901 ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac
     9961 ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt
    10021 aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt
    10081 caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact
    10141 tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc
    10201 gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt
    10261 tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt
    10321 tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc
    10381 cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat
    10441 tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata
    10501 tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat
    10561 ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc
    10621 ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct
    10681 tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta
    10741 catattgaga aataattaag gattacagcc tttactttat catgaaataa aagggaaaga
    10801 taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt
    10861 tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat
    10921 ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct
    10981 tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct
    11041 ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga
    11101 acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt
    11161 aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt
    11221 cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt
    11281 ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg
    11341 gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg
    11401 accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg
    11461 ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc
    11521 attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc
    11581 ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca
    11641 atctctgtca caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt
    11701 atctctttca tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt
    11761 atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa
    11821 acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt
    11881 tttctttaag tttacgttta tgtctttatt ttgacaaaaa aaatgtcttt tcgttttggc
    11941 gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa
    12001 aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta
    12061 aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt
    12121 tacgccagga attaaaagcg caaaaagatt acgaaggctt gcataaactt cctttagatg
    12181 ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc
    12241 gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag
    12301 gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat
    12361 ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta
    12421 ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc
    12481 atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat
    12541 tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt
    12601 caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat
    12661 tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc
    12721 cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac
    12781 caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata
    12841 aatatctcct ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct
    12901 gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca
    12961 tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt
    13021 cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt
    13081 tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga
    13141 tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc
    13201 cttcacctgc tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct
    13261 gcaatggata aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc
    13321 cataacgaat catattgccg attgtttcat catgccataa agccgttgca ctattgctga
    13381 catgtacata tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt
    13441 gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt
    13501 cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg
    13561 tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct
    13621 ctagcttact tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag
    13681 ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga
    13741 taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa
    13801 cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac
    13861 cgtatccgtt cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt
    13921 catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc
    13981 aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg
    14041 tttcatttgt atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa
    14101 caggctgacc attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc
    14161 cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta
    14221 cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg
    14281 caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga
    14341 tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct
    14401 accaaattat aaccattttg ccac
//
LOCUS       DAXWLH010000067        14212 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXWLH010000067 DAXWLH010000000
VERSION     DAXWLH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.067"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..108
                     /locus_tag="ACGE36_001508"
     CDS             <1..108
                     /locus_tag="ACGE36_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFF8383903.1"
                     /translation="GLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(170..1981)
                     /locus_tag="ACGE36_001509"
     CDS             complement(170..1981)
                     /locus_tag="ACGE36_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFF8383904.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1978..2343)
                     /locus_tag="ACGE36_001510"
     CDS             complement(1978..2343)
                     /locus_tag="ACGE36_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFF8383905.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2506..2901)
                     /locus_tag="ACGE36_001511"
     CDS             complement(2506..2901)
                     /locus_tag="ACGE36_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8383906.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2919..3881)
                     /locus_tag="ACGE36_001512"
     CDS             complement(2919..3881)
                     /locus_tag="ACGE36_001512"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFF8383907.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3881..4093)
                     /locus_tag="ACGE36_001513"
     CDS             complement(3881..4093)
                     /locus_tag="ACGE36_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFF8383908.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4114..4812)
                     /locus_tag="ACGE36_001514"
     CDS             complement(4114..4812)
                     /locus_tag="ACGE36_001514"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8383909.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4832..5374)
                     /locus_tag="ACGE36_001515"
     CDS             complement(4832..5374)
                     /locus_tag="ACGE36_001515"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFF8383910.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5506..6510)
                     /locus_tag="ACGE36_001516"
     CDS             complement(5506..6510)
                     /locus_tag="ACGE36_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8383911.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6507..7496)
                     /locus_tag="ACGE36_001517"
     CDS             complement(6507..7496)
                     /locus_tag="ACGE36_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8383912.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7493..8299)
                     /locus_tag="ACGE36_001518"
     CDS             complement(7493..8299)
                     /locus_tag="ACGE36_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383913.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8465..9421
                     /locus_tag="ACGE36_001519"
     CDS             8465..9421
                     /locus_tag="ACGE36_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8383914.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9498..10016)
                     /locus_tag="ACGE36_001520"
     CDS             complement(9498..10016)
                     /locus_tag="ACGE36_001520"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8383915.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10104..10253)
                     /gene="rpmG"
                     /locus_tag="ACGE36_001521"
     CDS             complement(10104..10253)
                     /gene="rpmG"
                     /locus_tag="ACGE36_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8383916.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10481..10927
                     /locus_tag="ACGE36_001522"
     CDS             10481..10927
                     /locus_tag="ACGE36_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFF8383917.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11122..13017)
                     /gene="asnB"
                     /locus_tag="ACGE36_001523"
     CDS             complement(11122..13017)
                     /gene="asnB"
                     /locus_tag="ACGE36_001523"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFF8383918.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13342..>14212)
                     /gene="bsh"
                     /locus_tag="ACGE36_001524"
     CDS             complement(13342..>14212)
                     /gene="bsh"
                     /locus_tag="ACGE36_001524"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8383919.1"
                     /translation="RFDFRKVKNLDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLN
                     FPGNADYKELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATINLVNIDFSEKLPLSP
                     LHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENH
                     FSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNAASGDSESESISQFFHILGS
                     VEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVS
                     YPVIKEQQINYVN"
BASE COUNT         4428 a         2997 c         2408 g         4379 t
ORIGIN      
        1 ggtttggtcg cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg
       61 atcgaagaaa accgtaaaaa agcaatgcgc aaacgcgttt ctcgttaata attatttaaa
      121 aacacgcatg aagacttgtg aaagaacaga taagtcatca tgcgtatttt tatttgtcag
      181 tcaatataag ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc
      241 tagctactgc tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt
      301 cagactggat ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag
      361 cttctgaatg ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt
      421 ccttcaaatt ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag
      481 cttcaatcaa atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt
      541 gcttttgctg aatatcaaat ggcggttcga acaaatgaaa aatacccgca tctatcagtg
      601 ctgctaattc tttcgtccga atgactggcg gaccgatcgt taaaaagcta tttagatgag
      661 taaaccatcc ccatagtatt tctttgtatt cctttgcaga aaaaacgtgc cattcaatag
      721 cctgccggat tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg
      781 tgcaattgcc tttttccgct tcttttatct gcccttcaat aaatgctcgg ctagcttctg
      841 caaaggactc atcctgatag gtaagaggaa tttctaaata cgaccaatcc catctataag
      901 ggattagtgc tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct
      961 caaaattttt ctttggaatc ggcaaatgat attcttcgat gattttcttg taataaaaca
     1021 actcaacgtc ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat
     1081 gccattgctc caggttttct tttgttagta accgtggcca tgccattgat ccgccttgct
     1141 tttggtttct tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg
     1201 gttccttgcc tgatggatga tagacaagtt ttcctttttc gttctcgaag cgcccttctc
     1261 ttgatactgt aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga
     1321 cattttcttt aggcggtatc ttcgaaagat cgacatctgc aggattagaa ggaagctgat
     1381 acagtaaatc atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat
     1441 tttcccagtg accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct
     1501 gtaaaagata accatctttt cttttgctga ctttttttac taaagaataa acaaactgaa
     1561 tagaagctgg aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata
     1621 aacatcgagt agattgctga tctttttcca gacgattgat ttcttctata attagttgct
     1681 tatttttggt taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca
     1741 agttgggtcc ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca
     1801 cttgttgaga aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac
     1861 ctccaggacc attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat
     1921 ggagaatcct ttctgctata cttaatcctc taggtccggc accaatgatc cctattttca
     1981 tttgcatgcc tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt
     2041 ttttgcacca taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa
     2101 aatagcttgc ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga
     2161 agcaaatcgt tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc
     2221 tttcaaccac agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaacctatc
     2281 cacgagtatt ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat
     2341 catagttgtt tccttctttc tgccttttcg cttagcttat atatgtagat aatagcacaa
     2401 aataaaaaaa atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat
     2461 ctaatgattg attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact
     2521 tttgattttt ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc
     2581 tgctttacgt aatttatttg ctgtacggat gctcaagctt ttcttttgat cgtaaatata
     2641 gacaggctgg tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt
     2701 tcttgcacct aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg
     2761 tgcttttctc attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgcttggc
     2821 cattactcgt agatagactt cccaaaagat gattgcaagc acaatcaata gcaaaatgat
     2881 attaattacc cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat
     2941 gaggcagcac caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat
     3001 tctcttactt gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat
     3061 tctcctgctg ccgatactcc tccacctaac acaatacttg acggattcaa tgtattcccc
     3121 aagttggcgc aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac
     3181 ggatcgtctt cttgagccat ttcaaagata tctttacttg ttacttcttc accgttgtct
     3241 aacattgctt tcagttttga atctcctgcg tattcttctg ccaagtaacg acctagacgt
     3301 accacaccag tagcgctaga cacagtttct aaacatccgc gtttaccgca agtacattcg
     3361 aacccttcag gatccacagt gatatggccg atctctccgc ctgctccgct cacgccgtga
     3421 atcaaatttc cgccagcaac gataccaccg ccaacacctg ttcctagagt aacgaataca
     3481 acgtctggat cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgca
     3541 tcattatcga tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga
     3601 acttctgtcc agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt
     3661 cctggggtac ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa
     3721 cgatggttga ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg
     3781 gtatcgatgc tccatttttg ttgaatctct ccttctggtg ttaaaattgc aaattttgct
     3841 gttgttccac ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc
     3901 ggctttcttc aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat
     3961 gatcaatcaa acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat
     4021 agatgcgtgc acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat
     4081 ataagctctc catctttaag cacttccttc atattataca agtaaaccat atttttttaa
     4141 acccagtaaa atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg
     4201 gtgtatcgaa tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc
     4261 gccaattagc ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac
     4321 aaatgtcaac acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg
     4381 tttaaaatga aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtactcgc
     4441 accaccagac agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc
     4501 gctgaacata taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc
     4561 cataaaatac agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat
     4621 tggtgtcact aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc
     4681 ataataaggg atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat
     4741 ccctaaaaaa gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg
     4801 ttgataattc atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct
     4861 gatcaaagct ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg
     4921 tttttttatg aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat
     4981 aaccggctct attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat
     5041 agagcttctc tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt
     5101 catccatttc ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct
     5161 tttgttcttt ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata
     5221 aagtcgtccc tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga
     5281 ttcgctgttc tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc
     5341 ctttccggcg taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa
     5401 atggtatagg aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat
     5461 aaaaagaaga cctagaaatg attggccttc cttttttaat aaatttcaac tgttcagcgc
     5521 gtgtttttgt ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa
     5581 aattcctgta ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag
     5641 cagaaaaatg gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac
     5701 gactagcttc gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc
     5761 tacactaccg cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac
     5821 gttaaggcct aatccttgcg cgcgttcttg accaaatgat agcaatccaa tcctctgtgc
     5881 ataaagtcct cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg
     5941 ccagctcgca ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt
     6001 agataattgg atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc
     6061 ggacaataag attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac
     6121 acagcaagca gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga
     6181 atagtgcgaa aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc
     6241 caatatacta gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac
     6301 gccaagacaa gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac
     6361 aatcagcgcc aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta
     6421 aataaaaggc gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa
     6481 gaaatagaac gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa
     6541 aacggaacgc caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc
     6601 atcctcgctg taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt
     6661 agaacataac gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca
     6721 ataaatgaaa caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag
     6781 aaaacaatca accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc
     6841 aaatgaatca aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat
     6901 aaaggaaaca atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa
     6961 aaagcaagat cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa
     7021 aaagaactaa tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa
     7081 aactgcaaaa aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga
     7141 aacacagaaa aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa
     7201 gcacccgcgt tgattcctag taagcctgaa tctgctaaag gatttcttgt cactccttgc
     7261 atcaatgcac cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc
     7321 aaacgaatat ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc
     7381 tgccaaaaat ctgcttttcc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata
     7441 atcaaaagaa caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt
     7501 gatcctcctg ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg
     7561 gaagcgtgac aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca
     7621 cttcttcagg tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg
     7681 aaaaacgtga agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt
     7741 gattgatttc agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg
     7801 tcggttcatc caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac
     7861 gctgggtttg tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg
     7921 tagcatccaa cgcccactcg attgcttcat gatcttgcgt gtccatgcca ctgaacagtt
     7981 tttgataagg gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt
     8041 cactagtttg tgctaataat gtaatttttt ttgcaagttc ttttgaactg tatgtagaga
     8101 tatcagagtt attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt
     8161 tcaataaagt agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag
     8221 gaatcgtaac agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag
     8281 ctgctcttaa tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg
     8341 ctgtttcaac atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca
     8401 gcgttcgaaa tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa
     8461 agaaatgaaa ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt
     8521 actgagcgct tgctccccct cttcagataa agaaacagct acaactgcca attcgacgac
     8581 ttcatccaat agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa
     8641 cgcgcccaaa cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa
     8701 acctatcgcc cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt
     8761 aaaggatgtc ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc
     8821 tgatttgctg ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag
     8881 caaaatcgcg ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact
     8941 aaaagatgtc ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta
     9001 tcaaaaatcc atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc
     9061 tgcagtgttg tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg
     9121 aagaatcgta tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa
     9181 aaaagccact tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga
     9241 ctacatcatc cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa
     9301 gaatcttaaa gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct
     9361 atataacgga cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg
     9421 attttaacta aaaaaagaat cttcacgatt agtcgtgaga tgcttttttt cttattaaca
     9481 aactagacgc atagatttta ttcttctttt gaagcaaccc tattttttac tctttcccat
     9541 ttttgaaaac gatgggcata tagattttct tgatccgtta tcccctcata ctcctctata
     9601 agggcgaagt ctgaaaaatc aatattccca atatacgtat cgccttcaaa cgtgctatcg
     9661 atcaatgttc gccagatccg accagtgtaa ggaagaagtt cttgaaaaat ccgactacca
     9721 cccgaaacat agatatcttc tgagatcgtt tgactatacg acaaaagttc atcgatcgaa
     9781 tgcattactt ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata
     9841 tggcgataag gaagtgaaag atcccccatt ccttcatagg tctttcttcc catcactaaa
     9901 attcgatcca ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac
     9961 aaaccatttt tgccgatcag accattttta tcttgtgccc acatggaaat aaacatatta
    10021 tccgctcctt atgattttag atgttttgag ataaaaaaag tctcaattct ttgaccagca
    10081 aggaattgag aactttaaaa acattatttt gtttcacggt gcaaagtaac tttgcgttcg
    10141 cgtgggcaat atttttgttt ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt
    10201 agatagttac gttctttaca agaagtacac tctaaagtga tgtttacgcg catgtttttc
    10261 cctcccattt ttacaagaat cctctaagat gactctcaga cttgattatc ttaccatgat
    10321 tttttctaga ttgctagtgt ttttcacgat tttgttaagt aaaactactt gacgataaat
    10381 acaacggttt tacttctcaa acaaaatctt tttctttttg tataaatacc cttacacttg
    10441 tttatataga ttatgttttt taataaaggg ggatactaat atgccatgga acatgaagga
    10501 ctttcctgca tcaatgaaaa acttagataa attgacaaga aaaaaagcaa tcgacatcgc
    10561 aaatgcatta ttagatgaag ggtaccctga taaccgtgcg atcccaattg ccattgatca
    10621 agcaaaaaaa tggtatgaag atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa
    10681 aaatccaagc aagacggatg aacacgatac gaatcctcgt gctggaaaac tgcttgattc
    10741 agatgtgatc gtagaatatg aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa
    10801 ggcaagcaac cattttgata caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca
    10861 aaacaaagaa tcaactttgt tcatttataa aaaagatggg acaaaagaaa aagaaatcga
    10921 ttattgaaca tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga
    10981 aacaatggtg ttcctctgcc aactccgaac cataagtcat aatatctatg aaacgggaag
    11041 agctgttttt tgaaattgtt tcttatcact cagtgttaaa cggtaggaga aaacctcttt
    11101 tttattgata tttgattttc atcaaacagt cacatagtcg attgcttctg cttgcggaat
    11161 cgtttcatct acaaagtaga ctttatacca tgtcaagaaa ctgaagattg tccagatttt
    11221 tctttgctct tctttacgtc cttcgtgatg gtcatctagc aacttcatga ttttttcttg
    11281 atcaaaaaat tcttttgcaa aatctgcttc gaataatgct ttgacttgtt cgtagccgtg
    11341 ttcttctttc aaccaagctt tgattggcac agggaatcct aatttcacac gattcgacca
    11401 ttcttccgga agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt
    11461 caataaaaac ttacttggta tttgttgagc caatttcatg acttcgatat ccaataaagg
    11521 tactcggact tccaaagaac ttgccataga aagtttatct gcctttaaca agatatcttt
    11581 tggcatccat tgatgaagat cgacatattg catcttattg acttcatctt tgattccttc
    11641 tgttttctcg taatgaacag tcatgatttc atccacagat ggtgctgttt gatattttgg
    11701 tgttaacact tctgatgctt ctgattcttc gaacactcga gcatgaccga taaaatattc
    11761 ttttgcagga gcaagtgatt catataaatg gatccgtcca tggaaatttt tcatttttcc
    11821 gattttacgt cctaaattat agcgtgttcc ttttggtaat tttttcaatc cttgagcaat
    11881 cacacggatg aattttgaat tcgtatggaa tccatacgtt tggtatcccg caaacagttc
    11941 atccgcccct tctccagatt ggactacccg cacactttgt gaagcaagat ttgctaaaaa
    12001 gtacaaaggt acgcaagaag gatttgaatc aggttcatct aagtgatact ggatcaaagg
    12061 gaatgctttg aacgacatgt ctccgtcgat cactgctgct gtattatcta agtccaatag
    12121 ttcggttagt tttctggctt caatcgcttc attataggtt ttatcatcaa acccgattga
    12181 gaaagaatga tctggtttca atacagaggt tacataactg gaatcaaccc cgctggataa
    12241 gaaagaacca acttctacat cactgaccgt atgtgcttca atagaagctt gaacggtttc
    12301 gtcgattttt tcgatccatt cttgtcggga atgcgtttct tttgtttcaa aatctgcatc
    12361 ccaatattgc tgaatgtcta gttttccatc tttatacgta taataatggc cttcaggcaa
    12421 acgatagaca tctttgaaga atgtttcgcc gtttaatgga gaatattgga aagtcatata
    12481 aggtttcaac gcttctttgt tcaattcttt gttaaaatca ggatgaggca gaaaactttt
    12541 gatttctgaa ccaaacataa atgttccgtt catttctgca taataatatg gtttgatccc
    12601 aaagtggtct cttgcaccga ataactcatt cttttcatta tcccaaatag caaaagcaaa
    12661 cattccgcgg atcttttgca ataattctgt tccccattct tcgtaaccat gcaaaaggac
    12721 ttctgtatct gcatgtgttt gaaaaacgtg gccagctgca atcaattctt ctctcaaagg
    12781 ctggtaatta tagatttcgc cattaaaaat aatgatcttt gtccgatctt cattgtaaat
    12841 aggctgagta ccgccctcta agtcaataat acttaatctt ctgaatccta aagcaacatg
    12901 tttatcgata tattctcctg aactattagg tccacggtga acgattcgat ccatcattgt
    12961 attaataatt gtttttttgt tgtccttgtc gttaacaaaa cctacaatac cgcacattta
    13021 actttcacat cctaatataa aattactgtt tatttcattc atatgcatct tatcatagaa
    13081 taaatagcgg acgaatattt tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa
    13141 gtttctgttt ttacagaaaa taaagtccca atatctaatt taccatgttt tcttttttga
    13201 ggctacctta ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc aaaaaaaaca
    13261 gcaaaaaaag acacgaccta aagatcgtgt ctaattatga ctgtatcaga aaatatgata
    13321 ccaaacggat ctattcatta attaattgac atagtttatc tgttgctctt ttatgactgg
    13381 ataactgacc aattcatgac gatccaagtc ttctttattt atatttacag cagtaatttg
    13441 actgtcttca tacgtacgat aataataaat ccctttatca acgttacagc aagaagaata
    13501 aatcgtatat tcgtattttc cttctccagt atcgcatact cctttttgtt gctcgactga
    13561 gcctagtata tgaaaaaatt gactgatact ttcagattct gaatcacctg atgcagcatt
    13621 cattttagta aatgtggctt ttacaaatcg agaaacagaa gagagatctc caggtaagcc
    13681 caatcctccc atcccacgac tataaacatt caagctgatc tggtttgaaa aatgattttc
    13741 tggtgttcca tttgatagcg agcgataatt gttcagatta aataattgat aatcaaatgg
    13801 cggattattt gtcaacacac cgacaggatt atcatataaa tgaagtccat ctttcatact
    13861 ttcaattact atcgattttt ctttatcagc taataaccag tgtaaaggtg acaagggtag
    13921 tttttcacta aaatcgatat tgactagatt gatagtagcc aataattctt tagcttcgtc
    13981 tatggttgag cattgcccta gtatccatgg aataaactca aatggtgcaa cgttatcttt
    14041 tccttcttgt aattctttat aatcagcatt gccaggaaaa ttcagtcctg ccatactcaa
    14101 ccctttttca tttgtagcct cataatagag tggatagttg gataccccag ctgctattcc
    14161 tatcattgca taatgtttat ctaaattttt tacctttcta aaatcgaaac ga
//
LOCUS       DAXWLH010000068        13954 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXWLH010000068 DAXWLH010000000
VERSION     DAXWLH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.068"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..229)
                     /locus_tag="ACGE36_001525"
     CDS             complement(<1..229)
                     /locus_tag="ACGE36_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8383920.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFS"
     gene            complement(708..1427)
                     /locus_tag="ACGE36_001526"
     CDS             complement(708..1427)
                     /locus_tag="ACGE36_001526"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8383921.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1476..3122)
                     /locus_tag="ACGE36_001527"
     CDS             complement(1476..3122)
                     /locus_tag="ACGE36_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8383922.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3301..3891
                     /locus_tag="ACGE36_001528"
     CDS             3301..3891
                     /locus_tag="ACGE36_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8383923.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3983..5488
                     /locus_tag="ACGE36_001529"
     CDS             3983..5488
                     /locus_tag="ACGE36_001529"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFF8383924.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5572..6924)
                     /gene="celB"
                     /locus_tag="ACGE36_001530"
     CDS             complement(5572..6924)
                     /gene="celB"
                     /locus_tag="ACGE36_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8383925.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6924..7442)
                     /locus_tag="ACGE36_001531"
     CDS             complement(6924..7442)
                     /locus_tag="ACGE36_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383926.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7458..7784)
                     /locus_tag="ACGE36_001532"
     CDS             complement(7458..7784)
                     /locus_tag="ACGE36_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFF8383927.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7797..9776)
                     /locus_tag="ACGE36_001533"
     CDS             complement(7797..9776)
                     /locus_tag="ACGE36_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8383928.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9797..10111)
                     /locus_tag="ACGE36_001534"
     CDS             complement(9797..10111)
                     /locus_tag="ACGE36_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFF8383929.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10279..10572
                     /locus_tag="ACGE36_001535"
     CDS             10279..10572
                     /locus_tag="ACGE36_001535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFF8383930.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10650..11762)
                     /locus_tag="ACGE36_001536"
     CDS             complement(10650..11762)
                     /locus_tag="ACGE36_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8383931.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11915..12763
                     /locus_tag="ACGE36_001537"
                     /pseudo
     CDS             11915..12763
                     /locus_tag="ACGE36_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13116..>13954)
                     /locus_tag="ACGE36_001538"
     CDS             complement(13116..>13954)
                     /locus_tag="ACGE36_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8383932.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4359 a         2852 c         2386 g         4357 t
ORIGIN      
        1 aagaaaataa aaatttgaaa ttttcccaac ccacccaagg actttccctc aagctagcca
       61 agaacccatc tgctgaaata tgaaaatttt tgaatgctac gacattcgcc attacaggta
      121 tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc catcaggaca agtgctttat
      181 aacgccatag attcataaaa aaatgattga acccctttcg ttttttcatc taaaaacccc
      241 tttcgattca ttcgcaacgt aattatagcg ctttcaataa tgagaataaa taaacaaagt
      301 atagggaaaa tgaaacaaag tataggagag gttgtgaaat cagcgttttg gcctgagtat
      361 tagagaaaaa aacgggaaaa tagttcctca tattttgccg ttttttgagc taataccgag
      421 ggtcagctga ttgaacaccg tttatcagag gttgtgaatt cagcgttttg gcctgagtat
      481 tagggagatc gtgtccttac tatctaactc tgagtcacaa ggaacaatgg ggactgtaac
      541 aaaagtcttg tcacagtccc ttattccgaa caaacggtgt tccagaagta gcttctacgg
      601 aaataagccg aaatttcaca aaaaagccat ccgaaagtca gacggacaag tctgcttttc
      661 agatggcgct acaactactc tgcttattct tttagctatc cattcaatta tttttctctc
      721 acttgttcta gctcttcttt ggtcatgggt cgatattgcc ctttattcaa atctttaggc
      781 agccaaaatt ttcccatacg gattcttttt aaatataaga cttcttttcc tactgctttg
      841 accattcgct taacttggtg aaactttcct tcatgcaaaa tcagacggat aattgaagtt
      901 tctgtctctt gatctacttg atcaatttgg agttttgccg gcaatgtctg ctcatcgcca
      961 tcaataatca gtccttctgc aaataaacgg atatcttctt ctgtcattat accgctaacc
     1021 tcagctagat attctttctc cacatgtttt ttaggtgata gtaactgatg ggcaaacacc
     1081 ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc gactggaaaa
     1141 agatcttccc gataatcctg gtcttccaat aaatcgatta cggtttgatc atgattatcc
     1201 tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc tttttgatac
     1261 gtaatgattt ctcctaaata agtaaccgca tcttcgaatt catttacttg cgctttatcg
     1321 cttttcacca cttgtccatt gacttcgatc gttccttttt tgattaatcc tttgacttct
     1381 ttgcggctgc caagtcccat atctgctaaa aacttatcta aacgcatgcg tttcccccat
     1441 ttcaaaagag catcaatcta gctcttctga ttcacttatt ttatttttaa tttatgtcgt
     1501 aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc ggattttcaa agctaaataa
     1561 ccatacacta aagcccctat acctgctact acaagaatca acaatagaga ctggaatttg
     1621 ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga tccacgaagc caaaagcata
     1681 atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga caaaacttgg tttaaagcgg
     1741 gctacttcat ggattctctt taagatcaag gtacaagaca cagcaaatcc gatcattgtc
     1801 gccaataaag ggccgtatac ttcaaataaa cgaatggctg ggtattgaag aaggagtttc
     1861 actagaaacc caataaccaa ataattaatt gccgccttgt tttcaaacat tccttgcagc
     1921 atgttggaaa ctagcatata tagtcctagg aagagtccta caaaacttgc ttggatcagt
     1981 acattacttc ctagagaatc cggtgtataa aataatgtat ttagcggata ggcaagtaac
     2041 ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt tccgcttgtc
     2101 aattttgcca gacctactcg atctttcaac gtaacagctt ctgtaatcaa cggtaaccca
     2161 gtagacgcga tcgatgttgc caatgcaata actaccatcg tcaacttatc tggattggca
     2221 ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat actcggtgga tgcagacatg
     2281 atcttcataa aagtgaactg atcgactaat ttgaagatcg ttacccctga acccacaata
     2341 ataaacggaa tcgcttcttt aacggtatct aacaacaatt ccttcgttgc aatatttatt
     2401 ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt gatgtttctt caaaaaatac
     2461 aaaaggacaa gaatacttgc tagcataccg ataaatgccg cgaaagttga ttgcgtgacc
     2521 gccgtcacat agtcaccatc catgactttc atgatgatga atgtagccaa tagcatataa
     2581 aaaatacgtg ccacctgttc tacaatctgc gacagcgcat aaggcatcat ttcttgattg
     2641 ccttgcaaat accctcgtat cacactcata caaggaaaaa ccaatacagc gatactcaaa
     2701 gaacgcatcg ttggaatcag ttcttctccg ccaccagaag catgagctag ccaaggagag
     2761 gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc ccatcatctg taatgcacgg
     2821 ataaataatc gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc agtttgtttt
     2881 gcaatcgcag caggtattcc tgctgtagag atcagtagaa acaatgcata tatattgtat
     2941 cccatattga ataatccatt ggcagcctta gcattttcgc ccatccaagc ataccaggga
     3001 atgatataga cagcgcctag caatcttgaa atgatattac tggcggtcat ccaagcagag
     3061 cctcgtgcca ttttttcttg cggtgttaat tgatctaatg ctggttttct ttgattatcc
     3121 atcggtcaga ttccaacttt ctacaaaaca ttactatcat tttaattggt aaaacagaaa
     3181 ctggcaatcg attttatata acttttataa gaacaattgt ttttttgaat tcggtttcgt
     3241 ttttttggta aagtaaagaa atatacagcc ttttatggct aacatttata ggagatatca
     3301 atgacacaag aaataaaaaa actacccgtt atatttttag gtcttttctt tatcacagtt
     3361 ggattgaatt ggttcttgct ccctcacgat atagcttccg ccggagtggg cgccatcgga
     3421 catttagttg aaacggcctt ttctatccaa cgctcagtta ctgtttggag catcaatctt
     3481 atgatgctgc ttctagccgc tgtcctatta ggaaaatcag tattttttaa gactgtagcg
     3541 ggaagtctgc tttttcctgt tgttttagag attgttccaa aagtggaaat gatctcctca
     3601 catttctttt cactgatttc aggaagtctt ttgttcagtc ttggggttta cacattgtac
     3661 acagtcgggg cctcaaatgg cggtataacg ataccgccga ttattttcca caaatttttt
     3721 cggcttccaa tgccgcaagg acttttactt accaatagtc ttatcgtctt tttgaattat
     3781 tatgtttttg gattgttgga gacactattc gttctcctat ctattacagt cagttcattg
     3841 tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa aatttgaata aagctggatt
     3901 ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg ctataataaa ccaagaaatt
     3961 gaataaagcg agggatcaat caatgagctt ctctttagaa gacatccgtc atttactatt
     4021 aaaagaaaat ctattaaaag aatttgtttc aacaagaggc tggcatcttg atgtaccaga
     4081 aaaagctgaa tttcttcagt taagttatga ttcaagaaaa gctgatgctt ctactttgtt
     4141 tttctgtaaa ggaatgaatt tcaaagaaga atatttggat tcagcaatcg aacagggtat
     4201 tcaatattat gtatcagaac aaccatacga aaattcagca gtaggaatca tcgtaacgga
     4261 tatccgtaaa gcaatggcgc ttcttgctat ggctttttac gactatccgc aaaataaatt
     4321 gaaagtgatc ggattcactg gaacaaaagg taaaacaact gctgcgtact ttacaaaagc
     4381 aatcttggat cacacaacaa ataaaaaaac agcattgctt tctactatga atacaacgtt
     4441 agatggcaaa acatatttca aatcccattt gacaacgcct gagtcattag atttatatcg
     4501 tatgatgtca gaagcagttg aaaatggtat gactcatcta gtgatggaag tttcttcaca
     4561 agcttacaaa acacaacgcg tatacggctt gactttagat gtcggaatct tcttgaatat
     4621 ctcgcctgac catatcagtc cgatcgaaca tccgacattt gatgactatt tctattgcaa
     4681 gcgacaattg atcttgaatt caaaaacagt tgttttaaat catgaaagtg actattttga
     4741 tttacttagg gaaacagccg atctattcca gattccaacg atcacttatg gtcgttcgga
     4801 agaaagcaat tatcaagtga tccgttcaga taaaggtact cacggtttta ctttatcttc
     4861 tcatgaagat cgcttagcta tctgcgatac tgcttatgat attttgctgg ctggaggatt
     4921 caaccaagaa aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg cctccagtca
     4981 agatgcacaa gacggattaa aagaagctcg tgtaccaggc cgaatggatc aattgatcca
     5041 atccaatgga gcgcatgtct atgtggacta tgcacataat tatctaagct tgaaaacatt
     5101 gctgaaattt gccaaaaatg aacatccaga tggccgtgta attgttgtat taggcagccc
     5161 aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa gtgctatcag aaacagctga
     5221 catcgccttt ttgacggcag atgacccagc atttgaagat ccgaaaaaaa tcgcggaaga
     5281 aatcaatgaa gcaattacta atccagattt gatcgttcat tatgaaatgg atcgtcctga
     5341 agcgattcga cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta ttgcgggaaa
     5401 aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag gggattatgc
     5461 aatcgtgaag cggttgattg aagaataatg gtatttatat actagtggtt tcccatttct
     5521 aagaaaaaaa gggactgtga ttttttgtca cagttccttt tttcttagaa atcattttaa
     5581 cttttcacta gctaattctg ctgccttttc tctttcttca tttaataatt tattatcata
     5641 gaatttgata aatgggaacc agataatgaa tgcgatgatt gcacaaatga ctgctaacac
     5701 agcagctttc caatcaccac cactaccaac aaatgctccg atcccgactg gtgaaggcca
     5761 cggcatctgt gcaagcatcg gtttgacgat ttccagccga atggcaaaat aagcgagtgt
     5821 agcagaagcc atcggagcta agaaaaacgg aaccgctaaa tagggattgt agacgattgg
     5881 catcccgaaa atgattggtt cattgatatt gaataatcct ggtacaagtg aggcttttcc
     5941 taacactttc agctgatcag atttcgcgag gaatgcaata aagatgatca atcctaaagt
     6001 cgcacctgat ccaccaacgg ttacataaga attgttgaat tctcctgcca acggataatt
     6061 tgcgccttgt gcgttagctg ccatattagc taatacgatc ggggtcaaga aagaagtgat
     6121 gatattcgct ccatggatcc ctacgatcca caatgcatgg atcaagaaat aaatcaccat
     6181 gacaccaagc catgtattcg tcaactgcgt aacaaaccca aatgggatag caatcatttt
     6241 gaagatatcc gttcctagtg caactaataa tccattgatg atcatcacca caaacgcaat
     6301 gacaaatgtt gggatcagag cagtgaaaga acgagaaact cctggcggaa caacatctgg
     6361 catttttatg acccaatttc tcttgacaca catacaatac aattcgaccg caataatcga
     6421 cataataata gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct
     6481 aataccattt actacagttt ctgtctcatt gatagaagca atcaagctga ttttaccatc
     6541 agtcatgatc aattcaggga tacacataaa gaatgcaaaa acagatagta atgcccccgt
     6601 gagcggatta acatttaatt tttgttcatc tcgttcaatt gacgttaatt cataagcaaa
     6661 cacaatggca aagtaaatag ataaaatccc catcgtcatt gtattgacga tcatgtacaa
     6721 gtcactaact ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga acggtaatgg
     6781 caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag aagccatccc
     6841 cgctgccatg acagcccgaa cgattttaag ttgcgcaatt ttccccatag gtcccatcag
     6901 gtatttctgc aaaaaatcaa acattagtta ttctcctttc ctaaatataa cgctttttca
     6961 tattgtttaa tacggatata ctgttggtct tggttgagtg aaattttctt taaacgatac
     7021 tgtataaatg cactggcaaa aataccgctt acaacaagaa tgaaaacaaa taactgtcct
     7081 ccattgttat ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa caacactgga
     7141 atcactaata aattaaagac actctggaac aaaaaatacc actttgtcca tggaacgttc
     7201 gtttggtgtt ttcgataaag ctttacttgt tctcctactg caggcaaaat agctaaaatc
     7261 agaatcatcg gtaaactact tcctaaactt ttagctgata gaagcagaat catccattgc
     7321 atgttcatga aaaagaaaaa ggcagtcagg taacgtacga ttagataacg attgaaatac
     7381 atgtttttca aactaagttg ctttttttct aattcgattt tcctttttac tgtatcctgc
     7441 acaatgatcc ttcctttcta tttatttttt tgatacaaat aattcatttc ctgtgcaact
     7501 tccaataatg tcatcgtcgt catcagatga tcttgtgcat ggaccatgat aatttccatt
     7561 tctatttttg tcccgctggc atattctttc agaagttgag tttgtgactg atgcgcatga
     7621 agaatctctt catttgcttg tcgcaacttc tcttctactt cttcaaacat ctctttacgc
     7681 atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa aatgatatca
     7741 aaagaagtca cttgtagtgt ttcactgttt attttttcag ccatctgttt tcctccttat
     7801 tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa taggttcagc taagatttcc
     7861 tgctgtactt ttagctggtc aactaaccta acgattgctt tagtcacgac cgtaattcct
     7921 tcattttcga cataagaagg cgaaactaaa aaaacaaaat gaatttcttg atattgatcc
     7981 cactgcatcc cagaaggaat caatgcaacg gctacctttg tttcgattcc aactggaaca
     8041 gcgggatgtg ggactgccac tgtatcactg aatatgattt gtcccatatt ttcacgttgc
     8101 tggatctgct ttcgcatttc tcttatatat ccttcttctt catttatgct cagcttttgc
     8161 agcaaatcat gaagaatttc ttcctttttc ggttcgtacg tataagtctt gaaaaatttt
     8221 tcagacaact gttcggtgta aatctgcttt ttttgctgta gagaaaatgc tggtatcggt
     8281 ctatctgagg aatgataggg tatagattct ccaactacct ttcgtatttt acgaacatca
     8341 tcttcattca aaaaaatact gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga
     8401 tcaatagaag aaataatcag gtcgattcct tttaaagttg tttcattgat ctcataatag
     8461 ccttttacac ttaccactgt aatattttta ccgaattcac tgactacgcg attctttaat
     8521 agttgggcac tgccgtaacc cgttgcacag ataatcaatg catgtagttt atgcgcagct
     8581 ctttcttttt ccaatgccgc catgagatgc aaagccaaat aggcccattc atcttcattg
     8641 atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc gtcaaagact
     8701 ttgggatttt ctttctttat ctcctcagtc aatgggttct caatgcgaat acctcggctc
     8761 aatcgaatca tcatcggtga caaatgatcg agtagtccat tgaaaagctg gagatcctct
     8821 actaatgaaa aatgaaattc ctgagacaat cttttcagaa ctttcgctaa ttcctttgat
     8881 agttcctgat cattttgatt ttctttgtga ttcgacttag ccatgagatg taatgtcaaa
     8941 taagcaattt cttcttcggg aaacgataca cctgcgatta cctcaatacg ttgaacgatc
     9001 cgctgcgcca cttgatactc tactcgctta gaaacttctt gttcgatgcc taattccttg
     9061 atttcaaacc cttctcggag ccgtttgatc cccaaagcca agtgtaaagt cagattctgt
     9121 atgataaaat ccgaaacttt taacttagct tctcttgttt catcgagaat gataatgact
     9181 agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa
     9241 tttccataat ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt tctttcttcc
     9301 ccttcgatcc atgtcccttg accatgctta gaaacgattt ttagtgaata tggaatcaat
     9361 cgctccttga tttcttgcat gtcctttgtc aaactggaac gactgatata aagctcttct
     9421 gccaaatcat ccataaaaag aatggcatct tccaaaagca attggttcaa tatatacttt
     9481 tgtcgatctt cggactcata aaatccttgc ggttctctag catatccagg tactatttct
     9541 tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa
     9601 cctggtttag caatgatctt acctccgttt ttttctacta actctttgat ccgctgcaag
     9661 taattacgga ttgtccgatc agataataac aattcagaag ctaaacgttg actggtgaca
     9721 tactccttct tgtgatcaat caaatatctc aacagcaatt tttccttctg tttcattatc
     9781 atccactcct attccatcat atgttctctt cgatcacctc tgctaacttc tggatgcctg
     9841 tagggattgg gacatacgct tggaaaggga tactaacaac aggctttcca acagattctc
     9901 ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa
     9961 aatcactatt cttaatcata ttatcgcctt ctgtagctga aactgcatct acgagtacct
    10021 cttctctttt ttgcttaaag aaatccgttg tctttgaggc catcatcgag gatgacattc
    10081 ctgccgcaca aataatcaat gcttttttca taaatcaatc actcctttta tttttataaa
    10141 ccaagcatat cttgtaagcg ttttctattc aaaatatata tttccgtagc attgcggaaa
    10201 aaatgtgtat catatcttga ataataattt ctggcaccgt gatatactag tgatagaatt
    10261 ttttcaggag gggatttagt gagtatcaca gtgaatattt tgtatacaag tacaaatgga
    10321 caagccagaa aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca
    10381 gaagaaggaa acgagaaata tgactattac tttccactag aagatccaga atctctttta
    10441 ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg
    10501 gctcaaataa acgaactccg taaaaagtat aaattaaaaa tgaaagtcga acgtttcact
    10561 gatttaccgt aaaaagatac acatggaatg atagcaattt atcatccatg tgtatcttca
    10621 ctttttcctt attttttatt cgatctcatc taatgattca aaccgtgttt ttctcttatt
    10681 tttcggataa gtatttcagt caccccaata tacatgattc ctaaacatac cccaaggata
    10741 ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga ataaactggc agtgattgcc
    10801 aaagcaccaa aagaattaaa aactgtttta tatctatagg tagaacaaac acctaaagct
    10861 agaccaccta atataccgac ccagttaaaa ggaataaatt ggctgatacc aataaataaa
    10921 acacatccaa tcaatgagcc aatgattcgg tctactgctc gttctttgaa accaatcgac
    10981 atcaatccat agctagatac aatagaagaa aatgcaaacc ctgcccacat caatcgctga
    11041 aaagggacat actctcctgc aacgatcaaa agactaatgc caaatgcata gatgagcatc
    11101 caaatttttt cctgcgaata catgcctttc aaaaaaatct ctttaaacaa gcttttgtct
    11161 ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat
    11221 aataagctac ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg taacgaataa
    11281 acaataaaga gatatgaaaa gccgtacaaa gaagcattcc ccattttggg atcactagcg
    11341 gttgccatta atatgctgct tagtaaaata aaatgaatca acatgcccat aactgaccat
    11401 ttgatcagct ggacatacgg tgctactaat agtgagaaca tgacgattgc taaacctatg
    11461 atcgaatggg aaatcttata accaaagtca acaaaacgaa tactcatcaa catacagaat
    11521 aaaaccacag catatggttt atgcttcgat tcaaataatg ttgtaattat aatgataaaa
    11581 caaatggcaa acaatacgat gaataatgat cgtaaaaaca atgaacaaat atacatgcac
    11641 ttttcttttt tagaagctgc ttctcgtatt ttttgtttta aaataaacgg atctagttgt
    11701 agagactgat aaaaatccac ctgttgtcgt cctttctttt tacttttatt ttttacagtc
    11761 atctatgaca ggttgtaaat actatggaac atcatatggc gttgtcattt tcttgtcaac
    11821 tatttttcct attcatgttt ttataaaaaa acatatcgtt ttcttgtatg attaaatcac
    11881 cacaaaataa acaactgaga gaacgatatg ctttatgaat ggttctatca aaaaaattct
    11941 gagatcaata gacaaaaatt tgatgattac agaggtctct tacgaaacct tacagaagaa
    12001 aaaaacattg atcgttgctg cagttctctt ccctgctcca tgtgtctgta gaaattgtgg
    12061 ttctgctgtg gtagatggaa ataggaaatt aatggtggtg aaaaacaaga agaaggaaac
    12121 cattgtccac tttgaataat ataatcatat gccaatggtt atacgcctaa aaaagcagcg
    12181 ctatacctgt aaaaactgtc gaagccattg gaacgctcaa agttatttta tccgtccaag
    12241 gcactcgatt tcaaatcatg tcagacataa aatcacttct ttactgactg aaaaagtgtc
    12301 gttatttttt atttcgaaaa gttgttaggt atctttgaca accgttattc gcacattgaa
    12361 agagttgaga agctatttac caaagtcttc caaaagaatt ctgtcttgag tgttgagggt
    12421 tgtgaatttc gttctcacgt ttccgttgag gataaactga gttttatttc cgcggatggc
    12481 gagacagaaa aattaatcga gaaaatttac ctacacaaaa aattcctcga ttaacaaact
    12541 atttccttag ctgcactaat cctaaagaaa tagaattctt agttacagac atgaacgcca
    12601 cctactttca gctcaccaaa catgttctac caaatgcgaa attggtgatt gatcgattac
    12661 ttggtttttc cacgcctttc aaggaggcgt aacctttatt cacgagttgg ttgaagcttt
    12721 ccgagacaaa agacccgact tgtttttctc cttattgaca aagagccatt aaaactagag
    12781 aaaaaattac aactgatcgc tgattatttg tagaaagaaa atcaaagtat tacccaataa
    12841 acttgagtca ttaggcaaaa aacactgcga aactcaagaa tgattttcgc agtgtttttg
    12901 tatatttttt attaactagt attactacaa aggtttattt ttcacttctc aacgcagcac
    12961 aaaattgtct ttacttattt actggttggg ttcaaccttc tctactccga tactaatatt
    13021 tgtttgtata ccccttctaa aatcggtagt ttccattcaa ttgatgggta ctagttgatt
    13081 ttaaaaacta tgcttattca attttttatt ttagctcata tatttcgttt ctttctaaat
    13141 tttatactga tgaaaacaag tagcacaccc aatattgaca atataaactg gctaatgatg
    13201 gtttctccag tttttggaag atgcttattt ccactgcttg ttggattgtt tcctcttata
    13261 tctgagctat cagcaacttt tctattctct tttgttaaaa agatcgccac ttcttgttct
    13321 ttttcgatct tgaaagttac tggtgtcgca tccaattcat accccgttgg cgcttgagtt
    13381 tctaccagct gatatgctcc tggtgctaag ccatctatcg ccagttttcc atctgctcct
    13441 gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa aacagctcct
    13501 tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt
    13561 cgattttctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc aaactcgatt
    13621 ggtgtcgcat ccaattcata ccccgttggc gcttgggttt ctaccagctg atacactcct
    13681 ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg tggttagacc tgactgcaga
    13741 acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc cccgctttgg
    13801 tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc ggtttgcttt cgtcaattct
    13861 accaccgctg tctgacttct ttcgatctca aactcgatcg gtgtcgcatc taattcatac
    13921 cccgttggcg cttgagtttc taccagctga tatg
//
LOCUS       DAXWLH010000069        13884 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXWLH010000069 DAXWLH010000000
VERSION     DAXWLH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.069"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            128..2422
                     /locus_tag="ACGE36_001539"
     CDS             128..2422
                     /locus_tag="ACGE36_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFF8383933.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2415..3080
                     /gene="pgmB"
                     /locus_tag="ACGE36_001540"
     CDS             2415..3080
                     /gene="pgmB"
                     /locus_tag="ACGE36_001540"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8383934.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            3073..4080
                     /locus_tag="ACGE36_001541"
     CDS             3073..4080
                     /locus_tag="ACGE36_001541"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8383935.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            4102..5121
                     /locus_tag="ACGE36_001542"
     CDS             4102..5121
                     /locus_tag="ACGE36_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8383936.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(5402..6769)
                     /gene="nhaC"
                     /locus_tag="ACGE36_001543"
     CDS             complement(5402..6769)
                     /gene="nhaC"
                     /locus_tag="ACGE36_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8383937.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(6878..8299)
                     /gene="tyrP"
                     /locus_tag="ACGE36_001544"
     CDS             complement(6878..8299)
                     /gene="tyrP"
                     /locus_tag="ACGE36_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFF8383938.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(8542..10419)
                     /gene="tdc"
                     /locus_tag="ACGE36_001545"
     CDS             complement(8542..10419)
                     /gene="tdc"
                     /locus_tag="ACGE36_001545"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8383939.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(10710..11966)
                     /gene="tyrS"
                     /locus_tag="ACGE36_001546"
     CDS             complement(10710..11966)
                     /gene="tyrS"
                     /locus_tag="ACGE36_001546"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFF8383940.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(12078..12283)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12546..13847
                     /locus_tag="ACGE36_001547"
     CDS             12546..13847
                     /locus_tag="ACGE36_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8383941.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
BASE COUNT         4347 a         2856 c         2609 g         4072 t
ORIGIN      
        1 tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata ttttcttttg
       61 tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc tcattaggag
      121 gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa tcagcacgac
      181 acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg gaaacggtta
      241 tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc aaggtactta
      301 tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa atggttatcc
      361 agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata tttcgatcaa
      421 cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg aattagatat
      481 gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata aagtacgcat
      541 ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct atttaactat
      601 agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg gaaatgttca
      661 aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg gaactataga
      721 agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg aacaatttac
      781 tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg actatcacga
      841 agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg agacatggtc
      901 agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc agcaagcaga
      961 aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa aagaagagca
     1021 aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag gagacgaaga
     1081 agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact acggcgaaga
     1141 tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg gagccactta
     1201 ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac ctgaagtaac
     1261 taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca acgctcagca
     1321 acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg aatgccataa
     1381 cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct atgcaatcta
     1441 caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac tggaagtttt
     1501 agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag caaataaata
     1561 catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata acaattggta
     1621 tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt taacttatca
     1681 aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag atatcgcaga
     1741 gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg acacattctt
     1801 agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc tgaatcagca
     1861 ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg tactacaggg
     1921 gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact ttgatttcta
     1981 tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga ttttagcagc
     2041 agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc gcttggattt
     2101 ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga ctggtagctg
     2161 gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt tgagattcgc
     2221 accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc gcggacgttt
     2281 attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag gttctcctct
     2341 agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa taacgctaag
     2401 aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc attaccgata
     2461 ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc attacgatcg
     2521 accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc tctttgcttt
     2581 tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa ttagcgaaaa
     2641 gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat gtcttccccg
     2701 gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct cttgcttctg
     2761 caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac tttgatgcta
     2821 ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc ctccttgcag
     2881 caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg caagccggta
     2941 tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct gaagaccttg
     3001 gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac ttgaaagaaa
     3061 agtggcatga ccatggataa tccagagatc tccgtcgtac cagttccaga gactgaaata
     3121 aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta cggtgcccgc
     3181 ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt attgtcttac
     3241 gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt cggacctgta
     3301 gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa ctgcggatca
     3361 caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt agaagttttc
     3421 aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc tacctatcca
     3481 ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga gatgacaacc
     3541 tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta cttcaacctt
     3601 tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt agatggtgtg
     3661 cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga aaaactgcca
     3721 atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc agatggacta
     3781 gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca attattcgag
     3841 aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt attattttct
     3901 acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc aaactacgga
     3961 ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt tggatcgatc
     4021 aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc tgcacaataa
     4081 caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa aaaagccggt
     4141 gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca
     4201 aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat gactgcccgc
     4261 aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct tgattcaaaa
     4321 gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga agaagcgcgg
     4381 aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct gttagagaac
     4441 gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta ttcagatagt
     4501 caagatactg tcattgatta tttataccaa aaccacatcc ctttcacttt gatcggacag
     4561 ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct tctaggtaaa
     4621 caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt gacaaacacg
     4681 actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat gatgatggct
     4741 ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct gtcctttgag
     4801 aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat cttcgccctt
     4861 cgcacgatgc agttggcgca aatgtatggc tatgtcattc cagagacact ctccgtcatt
     4921 agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag tatcgatatc
     4981 gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa
     5041 ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga gacagtcgtt
     5101 cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat ttatcctatg
     5161 gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct gtaaaaaaga
     5221 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     5281 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc
     5341 ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc
     5401 ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg
     5461 caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc atcgttccca
     5521 taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg
     5581 ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg
     5641 tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg attccgatac
     5701 agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt
     5761 gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta
     5821 aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta
     5881 gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga
     5941 ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta
     6001 atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca
     6061 atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag
     6121 ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga
     6181 atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa
     6241 tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac
     6301 cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca
     6361 aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa
     6421 ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat
     6481 aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca
     6541 aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga
     6601 tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa
     6661 ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca
     6721 acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc
     6781 tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta
     6841 aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct aaactttttt
     6901 ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga ataacgaaca
     6961 tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta cctaatgtag
     7021 caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa gctaatccgt
     7081 ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta cgcattttaa
     7141 gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta gcatttgtaa
     7201 gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca aataatgaaa
     7261 taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt tctttatgga
     7321 atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt tttgatggtc
     7381 cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag ctgaagatat
     7441 ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc tgcgtgatat
     7501 ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca ttgatgacat
     7561 tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt tttgttgcgt
     7621 ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata aatgtgatac
     7681 cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt gaccatgaaa
     7741 agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa acacctaaaa
     7801 cgaacatgat gactactggg atatataccc ctagccatac accgatgttc ccgccgactt
     7861 ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact aaaatacaac
     7921 ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt cctaacaatg
     7981 gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat aatagccatg
     8041 caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga ggaccgcctt
     8101 cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg gcaaaaaaca
     8161 aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac gtaggaatac
     8221 tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga cccattttac
     8281 ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa ataacggtgt
     8341 ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg ggattctttg
     8401 agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat cgaagagctt
     8461 aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta ttcaagctgt
     8521 tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt ctgcgtagat
     8581 tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt caaagtgttc
     8641 ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt ttccggcacg
     8701 gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc tgttgccata
     8761 gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa tgcttccttt
     8821 cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa ctaagtcatc
     8881 gttgcctttt tctttgaata catagtctac catattgaaa tctggataag ttaatggatg
     8941 aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt tgtagaaacg
     9001 atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat tcaaaggtaa
     9061 gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac cttccaagat
     9121 ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg tcgcaaagta
     9181 agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag aatatggaac
     9241 atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg ctttatatgc
     9301 actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt atttgtaatg
     9361 aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga aaatcgcacg
     9421 gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt ctttttctaa
     9481 tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt ctgttgatcc
     9541 tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac gaacgatttt
     9601 ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa tcacttggtc
     9661 taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag tttgtggaac
     9721 caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag ctttgatttc
     9781 gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg acaagttcat
     9841 taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca ttgcaagcgg
     9901 caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag aaccatcagc
     9961 aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca ttcctacttc
    10021 ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac cattccatag
    10081 catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca tatgacccca
    10141 gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg aaatttctga
    10201 tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt tttcttctgg
    10261 cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat gttcatccac
    10321 caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc caatgaatag
    10381 ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct cctaaaaaaa
    10441 tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa actaagtttt
    10501 ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc acacttttga
    10561 aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag attgtattaa
    10621 cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta atagtatttc
    10681 cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa aaagtaattt
    10741 ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg atttatgaca
    10801 aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga aacatcttca
    10861 cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac gatattttca
    10921 ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca
    10981 tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc ttctttcaag
    11041 tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg tgcttcacgt
    11101 ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg agacaagaaa
    11161 gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca gaattggtag
    11221 aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc agtttttccg
    11281 aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc ttctgcttct
    11341 ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca ttggtctgct
    11401 ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc
    11461 aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct ggcaacgata
    11521 tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa aaattcaagt
    11581 aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc atctttacca
    11641 aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt
    11701 tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt tccgccgcca
    11761 atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat aaatggaatc
    11821 aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc
    11881 ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc
    11941 caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt ttatgaaagc
    12001 aggaacctgc tagcatgtgt aattaattat ttcttcagga taggatagga aataaaaaaa
    12061 gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt tgtatgtcta
    12121 gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact gccaaagact
    12181 cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca gctttctgaa
    12241 acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta atatgtgtct
    12301 atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt aaattcctca
    12361 ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta ttgttaaaag
    12421 aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac tttgcctctt
    12481 attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag caagggacga
    12541 ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta actatcgtta
    12601 ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc
    12661 ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa caactagtta
    12721 ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg ttattggaaa
    12781 aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta aaaaaggaga
    12841 aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt ttccacagct
    12901 tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac aaagattata
    12961 tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat gattttgttt
    13021 tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc tttaacgggc
    13081 ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc agctattatg
    13141 aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac aatgaaatca
    13201 aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg gttcctctga
    13261 tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg
    13321 ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa tatggagaca
    13381 atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag
    13441 acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa gcttcactag
    13501 aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa
    13561 caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct tattcagcag
    13621 aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt aatgtcgatg
    13681 cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct cggatcaata
    13741 tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt gatccattca
    13801 tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt tacgtcattt
    13861 atgaaccaaa aaagccgaac tgac
//
LOCUS       DAXWLH010000070        13105 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXWLH010000070 DAXWLH010000000
VERSION     DAXWLH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.070"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(27..1505)
                     /locus_tag="ACGE36_001548"
     CDS             complement(27..1505)
                     /locus_tag="ACGE36_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383942.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1803..2738)
                     /gene="murB"
                     /locus_tag="ACGE36_001549"
     CDS             complement(1803..2738)
                     /gene="murB"
                     /locus_tag="ACGE36_001549"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFF8383943.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2811..3731)
                     /locus_tag="ACGE36_001550"
     CDS             complement(2811..3731)
                     /locus_tag="ACGE36_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8383944.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3844..4596
                     /locus_tag="ACGE36_001551"
     CDS             3844..4596
                     /locus_tag="ACGE36_001551"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFF8383945.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5460..5993
                     /locus_tag="ACGE36_001552"
     CDS             5460..5993
                     /locus_tag="ACGE36_001552"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFF8383946.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6483..7007)
                     /locus_tag="ACGE36_001553"
     CDS             complement(6483..7007)
                     /locus_tag="ACGE36_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8383947.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7000..7473)
                     /gene="tsaE"
                     /locus_tag="ACGE36_001554"
     CDS             complement(7000..7473)
                     /gene="tsaE"
                     /locus_tag="ACGE36_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFF8383948.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7596..8576)
                     /gene="pta"
                     /locus_tag="ACGE36_001555"
     CDS             complement(7596..8576)
                     /gene="pta"
                     /locus_tag="ACGE36_001555"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFF8383949.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8729..9409)
                     /locus_tag="ACGE36_001556"
     CDS             complement(8729..9409)
                     /locus_tag="ACGE36_001556"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8383950.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9535..10404
                     /locus_tag="ACGE36_001557"
     CDS             9535..10404
                     /locus_tag="ACGE36_001557"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8383951.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10430..10945
                     /locus_tag="ACGE36_001558"
     CDS             10430..10945
                     /locus_tag="ACGE36_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFF8383952.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(10992..11555)
                     /locus_tag="ACGE36_001559"
     CDS             complement(10992..11555)
                     /locus_tag="ACGE36_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383953.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11610..11789)
                     /locus_tag="ACGE36_001560"
     CDS             complement(11610..11789)
                     /locus_tag="ACGE36_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383954.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11866..12375)
                     /locus_tag="ACGE36_001561"
     CDS             complement(11866..12375)
                     /locus_tag="ACGE36_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8383955.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12471..12851)
                     /locus_tag="ACGE36_001562"
     CDS             complement(12471..12851)
                     /locus_tag="ACGE36_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8383956.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3830 a         2627 c         2251 g         4397 t
ORIGIN      
        1 tgagacaaag ctgccatcca taaatactag ctgatttttt cagttaaatg tctatttttt
       61 tctctgatga tggtatttcc aaaatattcc cagtcatctg gagtcggtgg atttttccaa
      121 attatctgtt ctttattaaa tttttcaagt aaacaatcag ataaataaat attcgtacga
      181 gaagtgattc gactctcaat cacaatattg taatttttaa atcctttcac ttgagaaata
      241 atgtaatcgg tataagcaga tccttgcgaa aaatcaatac agacatgaat cggtgcttca
      301 aaatatgata ccggacaaat accgaccaat aaaaagagat aatcataaaa taagcgaatc
      361 atgaaacgtt catcttttcc caagaaatag cgtttattct cttgtatgac cttttggatt
      421 gctgtactga atattggata cgtctcgaag aaaaactgta gctgcttttt agacgtaaag
      481 ctagtcatct caaagtcgaa tatccaattt tttcgattga catgcatcat acccatacga
      541 agttcttttc gtatatatag tctttcttct tcagagattt caatcgaaaa tggtaattga
      601 tcaatcaatt ccactgacat tttttgacta atcatgtgga tatcatgaaa atgagcttcc
      661 gcaagtccaa atgcgccatc taattcaaat tcttcttccg tgatcaaaaa caacatgata
      721 taagaaatct catcaatgat ctgctcttta gggatatgaa gaaaagaaat atgtgataaa
      781 tccttgatga tcttcattcc ttctgagctc ttaaagtatt catctgaaaa gaaacgtttt
      841 ccgattggtt gattcccata ggccctcaac aaactaatag tggttagcaa attgaatttg
      901 attcgttttg ttgcagacag ttttaaattg aacagatatt caaaatattg cacgatactc
      961 tttgcttcat tcatgacgct ttctctaaat ggcatagaaa aaccattaaa gaccccgaaa
     1021 tataccccaa ataatacgtt ccgtatgttt atttcttccc caactaatat gtttttcttt
     1081 aactttattt gatgctgttc aagaaaagag actaattctc ttctttttac ataggctctt
     1141 gatatgctta aaaaatgatt ctctgcaaat ttttcgatcg ttacttttga atccacgatc
     1201 tccgttaaca gtcgagcaat ttttgatcgt tgaaagtaga ttcttcttaa atttttgata
     1261 tccgtcaaat tcagacgttc gacatggatc tctgcttctt ctgaaatttg gatctctggc
     1321 tttgttttga attcagtaaa ttctttattt aattctatga tataatttct tgttttaaat
     1381 ttagacaaac ctaatgtttc atataactta ggcagcacta cataattctt tttctcaaat
     1441 tctaaatatc tagctagcag cagtttttct ctatcttcct cttctataag gtaatccaac
     1501 ttcatatcct acacctcaaa aaatgcctca taccaattac ttgatatgca gatgaaagtt
     1561 catcttttca cctatactga ttttatgatg gattatgctt gttttttcat cagttattct
     1621 cattttttat cttttttttg agaatattgc aaaaaatgga aaaatttatg aggaaatatt
     1681 ctatattttt tgttttattg tgttaaaatg tacaagattt gcgccatttg cgtaaacaac
     1741 cacaaaaaag agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact
     1801 tattaattgg ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc
     1861 acttctgttt ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc
     1921 aattcaatat aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt
     1981 tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga
     2041 cctgtatagt gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc
     2101 tgttttgatt gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc
     2161 cctgtttgca aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg
     2221 tgacgataag aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc
     2281 aaaagatcca cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata
     2341 taaaccgctc caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat
     2401 tctgccaaag ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc
     2461 gttcctttga catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg
     2521 ccatcttgaa cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt
     2581 cggcaataat cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca
     2641 gctggtccac ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta
     2701 atcgtggcta agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct
     2761 caaacgtcct cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga
     2821 tgacggtagt gatctttaag cacttcctca cataattcat gacttcttac aacttctttt
     2881 tgtttaagca attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc
     2941 attggctcaa acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt
     3001 tttccatcag catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt
     3061 ttacttgtta cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata
     3121 gccgtttgat tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct
     3181 cttatctggt attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata
     3241 agatgtaaaa acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt
     3301 ttttgcaggt ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca
     3361 aagcgccggt tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat
     3421 tcacgaactt catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt
     3481 tctagacatt ttttggctaa ttcataatgt acttttgttg ctgcgtgtat catacatgct
     3541 tcgatttttt cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct
     3601 ttttgaattc tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct
     3661 tgttttttcg cataagttgg taaatatgct ttttctgcga tattccctac accgattaca
     3721 ccaatcttca tctactttcc tcctggatca tttagttact ttcatcttac accatttttt
     3781 actaaaatga ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt
     3841 ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt
     3901 ttagaagttt ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa
     3961 gcaggacaaa ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga
     4021 aaaggctatt ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga
     4081 atgggtatcg atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc
     4141 tttcttgtaa cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga
     4201 ttagaatggg aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc
     4261 atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg
     4321 aatcaaaaaa atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat
     4381 aacgggttta tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg
     4441 tggaactacc gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg
     4501 acgagcaaag atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc
     4561 agtgatcatt gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag
     4621 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca
     4681 taataccgag aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct
     4741 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg
     4801 cttattaccg cagatacaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc
     4861 actgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt
     4921 gggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtgaaaaagc
     4981 tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg
     5041 gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa gttgtgaaat
     5101 cagcgtcctg acctgagtat tagagaaaca aatggcaaaa tagttcctca tattttgcta
     5161 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag aagttgtgaa
     5221 aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa atggttcctc acaactttgc
     5281 ataaatttga cttaataccg agaagtcatg atccccatct aactccaaat cacaaggaat
     5341 gagttcaaaa actagctttt ctcaacaaat attacataaa aaattctatg ttttttgtta
     5401 aatttatttg cttttttcaa aagtatgtta cagtaatttc ccaaggaggt tttacagaaa
     5461 tgaattttgt tgccatggat tttgaaactg ccaatcatca gccttatagt gcttgttctc
     5521 ttgcgctagt catggtaaaa aatagccaga tcgttgatga attctataca ttgatacagc
     5581 ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa gaagacgtac
     5641 gcaatgcgcc gaaattccca gaagtctggc aaactatcca accatatttt caaaaaaatc
     5701 gtttagtcgt tgcacataat gctgcttttg atacaaaggt cttagcggga tgcttagatt
     5761 attatcatct tgaacagcct aattatcttt ctttatgtac agtgaaaagt agccgtcgcc
     5821 tgtttcctga atttccaaat catcgattga acactgtgtg cgaaaatttg aacatccaat
     5881 tagatcatca ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc ctgctttatc
     5941 aagaaaaaaa attcgggaca gatccattga aaaaacttgt gactataaag taaaagaata
     6001 cttatgaaac acacaaagag gtcgtgagcc tgccgtttca ctccgagtca caaggagcaa
     6061 tttcaaaaaa tagcttctca tgtttattaa atattgtgac tggtgaccga ggagctggca
     6121 ggagaacacc gtttattgga gaggttgtga aaaagctgtt ctgcatcaag taataagaac
     6181 agccaaacaa aaatagcttc tcatattttt catttggttg gacttattac cgcagatgca
     6241 agcttttgga caccgtttac caagaggttg tgaaatcagc gttttgacct gagtattaga
     6301 gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggtc
     6361 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
     6421 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
     6481 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
     6541 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
     6601 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
     6661 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
     6721 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
     6781 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
     6841 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
     6901 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
     6961 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
     7021 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
     7081 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
     7141 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
     7201 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
     7261 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
     7321 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     7381 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     7441 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     7501 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     7561 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     7621 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     7681 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     7741 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     7801 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     7861 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     7921 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     7981 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     8041 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     8101 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     8161 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     8221 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     8281 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     8341 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     8401 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     8461 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     8521 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     8581 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     8641 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     8701 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     8761 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     8821 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     8881 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     8941 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     9001 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     9061 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     9121 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     9181 ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa
     9241 cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct
     9301 tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa
     9361 attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct
     9421 cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt caccctcagc
     9481 acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc
     9541 aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct
     9601 gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact
     9661 ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg
     9721 atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg
     9781 attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat
     9841 gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt
     9901 gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc
     9961 tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa
    10021 gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta
    10081 ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa
    10141 aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct
    10201 cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc
    10261 agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa
    10321 gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg
    10381 cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt
    10441 tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg
    10501 acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta
    10561 tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg
    10621 ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc
    10681 cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata tctataatca
    10741 tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt
    10801 ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg
    10861 tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt
    10921 ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca
    10981 ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc
    11041 tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca
    11101 tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct
    11161 aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa
    11221 taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga
    11281 tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa
    11341 ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg
    11401 taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct
    11461 gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt
    11521 tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct
    11581 aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta
    11641 atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact
    11701 ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt
    11761 ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc
    11821 ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca
    11881 tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt
    11941 gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt
    12001 catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc
    12061 ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac
    12121 ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt
    12181 ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc
    12241 tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc
    12301 ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa
    12361 catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga
    12421 acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc
    12481 agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta
    12541 tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca
    12601 atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt
    12661 tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat
    12721 ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta
    12781 acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt
    12841 gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag
    12901 ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa
    12961 ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat
    13021 gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt
    13081 tataattgaa agactagttt ttttt
//
LOCUS       DAXWLH010000071        12891 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXWLH010000071 DAXWLH010000000
VERSION     DAXWLH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.071"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            107..1393
                     /gene="efmA"
                     /locus_tag="ACGE36_001563"
     CDS             107..1393
                     /gene="efmA"
                     /locus_tag="ACGE36_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFF8383957.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVSYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1531..2130)
                     /locus_tag="ACGE36_001564"
     CDS             complement(1531..2130)
                     /locus_tag="ACGE36_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8383958.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2534..3655
                     /gene="mnmA"
                     /locus_tag="ACGE36_001565"
     CDS             2534..3655
                     /gene="mnmA"
                     /locus_tag="ACGE36_001565"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFF8383959.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3901..5322
                     /gene="arcD"
                     /locus_tag="ACGE36_001566"
     CDS             3901..5322
                     /gene="arcD"
                     /locus_tag="ACGE36_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFF8383960.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5336..6454
                     /locus_tag="ACGE36_001567"
     CDS             5336..6454
                     /locus_tag="ACGE36_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFF8383961.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6514..7806)
                     /locus_tag="ACGE36_001568"
     CDS             complement(6514..7806)
                     /locus_tag="ACGE36_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8383962.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7956..8516
                     /locus_tag="ACGE36_001569"
     CDS             7956..8516
                     /locus_tag="ACGE36_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8383963.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8645..9022
                     /locus_tag="ACGE36_001570"
     CDS             8645..9022
                     /locus_tag="ACGE36_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFF8383964.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8955..11027
                     /locus_tag="ACGE36_001571"
     CDS             8955..11027
                     /locus_tag="ACGE36_001571"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8383965.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11238..12185)
                     /locus_tag="ACGE36_001572"
     CDS             complement(11238..12185)
                     /locus_tag="ACGE36_001572"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8383966.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12431..>12891
                     /locus_tag="ACGE36_001573"
     CDS             12431..>12891
                     /locus_tag="ACGE36_001573"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFF8383967.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQ"
BASE COUNT         4154 a         2339 c         2752 g         3646 t
ORIGIN      
        1 tgaaacgatt gttatttagg gaaaaaggta ttttttgaaa aaagaatccc gtataatgat
       61 tgttgtgcaa acgctataca aaatgaaaaa gaaaagaggg atgtttatgg aaaatgaaca
      121 gtcggtcgtt ttaacgaatt ggaagagaaa ttatttgttc tttttatcgg gacagttttt
      181 gtcaggaatc actagtatgg tcgtccaata tgcgatcatc tggtatttga caagagaaac
      241 cggttcggcg acaattttga gttttgctac tttactagga atgattccca tggttttact
      301 aagcccattt gtaggaccgc tagtggatcg ctgggataaa aaagctcttt tgattgtcac
      361 ggatattatt gtagcgattt tcgcgcttat tttagcagta gtcggaacga tttcagaatc
      421 cttcccgatt tggctagttt ttgtgtcttt gtttatgcgt tcggtcgcac aaacattcca
      481 aatgccaacg atccagtcga ttatgccgac aatcgttcca tcttctcaca taacaaggac
      541 gaatggacag ttaggaatgg tccaatcagc gaatttcatc attgcgccag ctctaggtgc
      601 ggctttgttt tcagtcgttc ctgtcagtta ccttatttta ttagatgtat tgggggcagt
      661 ttttggtgtc ggcttattga tttttgtgaa gatcccaaaa gtttctcctg aaatattgga
      721 agttccgctt actattttta aagatgcaaa atttgggctg cagcaattga tggataataa
      781 agggttatgg tacataacga tcaatggtgc atttgtgatg ctgttgttca tgccagcaat
      841 cagtctgtat ccattgatga cgctagatta ctttggtggt tccgttggac aggcgggagc
      901 agtggaagta gtctacgcag tcggaatgct cttaggcggt gcactgatca gttttatagg
      961 aacatggaaa gaccgaatga aacctattat tatagcctac atcattatgg gcctgacgat
     1021 cggtgcaagc ggattggttc caaacgatag tcaagggttc ttgtactttc ttatcttgaa
     1081 tgcaggagca ggttgtgcaa cgccatattt caatacactg ctgatggcaa tgatccagca
     1141 gagttatgaa tcgaatgttt taggtcgtgt cttagggaac ttcaactctt tgatgaacct
     1201 ggcaggtcca attggattat tatttgcagg accattagct gatcgtttag gggtagaaaa
     1261 aatgttcttg ttttccggaa tcgggatttt attatgcgga atcgttttat ttttaacgtc
     1321 agctgcccga aattatgata aagaattaca gaaaaaactg gtaaaagaac accatgagca
     1381 aaaagacgaa tagtatggaa tggagttgtg acagaagtct tgttacaact ccatttctga
     1441 tcctaatgtt cctgtggcaa taagcctact caaatgaaaa atatgagtaa ttatctttat
     1501 ttgtttaata cttgatatag ggcagttttt tcacgaccta tcgtacttcc tctttgcttc
     1561 atagatttct ttgatttgtt gctcagataa cggagtgcca aatggtagtt tttcctttaa
     1621 gagttctctt tccggtgcgt ctacgcctac attgacatcg ttctcgatag gtacgctgta
     1681 atgatggata tgtccatgaa gattgcggat agtgtcgtta ggcccgagca acatcggata
     1741 atgagtcaag taatattgtt gatggtcgaa tttaacgatg gcacctacat cataaaaata
     1801 aaatttggga cttccgtcag taagcctagg atcatttttt tctaaataac ggaaaaaagc
     1861 gcgactgtca tggttccctt tgatcaagtg gattgttcct tgcagttgag tgaccagttc
     1921 caaaatttct gcatttgctt tttcatattg cggatgcata gcaaggtcgc ctagatgata
     1981 aacatggtcg gtttctttta ttactgcatt ccagctagtg atcaactgtt ggttcatttc
     2041 atccacattt gcaaagagcc gcggagcaaa gtcgtttttt cctaataagc gttcatggaa
     2101 aaagtgcata tcactgataa agtaattcat gtatcttttg cctccctttt ttcttagagt
     2161 aaacaaaatc acttatctga gcaactcata acatctaaaa agaaaaggtt gtaaatgaga
     2221 cgtgcagctt taagcgataa gaagcaattt tgaaaaatag tttttcacaa tttataaaat
     2281 tattccttat tgccgaaagg ctggcccttt ttatgagtat gccaattttt tatatcgaat
     2341 gctcaaaaga tgagtgattt tttggttgac tatcatgttt gtgtactgaa taggtccagt
     2401 agaacagtat ttttacgact tgataattaa gggatgaaat tttcagaaat atgacgtata
     2461 atgtaaaata ctgaaaagtt gcgaccttca actcgtagat gtttcagaat ctaattgaaa
     2521 tgatggtgat atcatgacag acaacagcaa aacacgcgtt gtcgtaggga tgagcggcgg
     2581 tgtcgactca tctgtaacag cacttttgtt aaaggaacaa ggatacgacg tgatcggaat
     2641 attcatgaaa aattgggatg acacggacga aaacggtgta tgcacggcca cggaagatta
     2701 taaggatgta gcgaaagtag ctgctcaaat cgggattcct tattactctg tgaattttga
     2761 aaaagaatat tgggatcgtg tatttgaata tttcctagcc gaataccgcg caggacgtac
     2821 acctaaccca gacgtgatgt gcaacaaaga aatcaaattc aaagcattct tggattatgc
     2881 aatggatctt ggcgccgatt atgtagctac tggacattat gcgcaagtca ctcgcgatga
     2941 aaacggcgtg gtccacatgc ttcgtggagt agacaataat aaagatcaaa cttacttcct
     3001 tagccaatta tcacaagaac agctttcaaa aacgatgttc cctctaggcg gcatggaaaa
     3061 atcagaagta cgggcaattg ctgaacgcgc aggccttgcg actgctaaga aaaaagattc
     3121 aacgggaatc tgctttattg gtgaaaagaa cttcaaacaa tttttgagca actatctgcc
     3181 agcaaaaaaa ggaaatatgg tcactcttga tggtgaagtc aaaggacaac atgctggctt
     3241 gatgtattat acgattggtc aacgtcaagg actagggatc ggtggcggag gagattcgca
     3301 agaaccctgg tttgttgtag gaaaagatct agcgacaaac acattatatg tggggcaagg
     3361 gttccatcat cctgcattgt atgccacaag cttggatgca agtgaaatcc actttacgac
     3421 gaatgaacca atgccaaaag aattcaagtg tacagcgaaa ttccgttatc gtcagcaaga
     3481 cgttcctgtg acagttcgct tattggatga taatcgtgca gaagtcgtat tcgacgaacc
     3541 tgtacgtgca atcactcctg gacaagcagt tgtattttat gatggaatgg aatgtctggg
     3601 tggtggtttg atcgaccacg catatcaaga aacaaaagtt ttacaatacg tttaagcgat
     3661 attaaatgaa tcaaaacagc tatggctaac aaaagaaaca ccttttcttt tggatagcat
     3721 agctgttttt ttgcaataaa attaattttt attcatttta tacttgaaat acttgtgtaa
     3781 aaagttcata atatccaata atatgaataa agttgctttt tgttaagcaa gagcagttgc
     3841 aaaatagaag ggaatctttt gcaagtgatt tgttcaattt tataagtgag ggtgatggaa
     3901 atggaaaaga aatcgacggg tattagcaaa tggggactgg ttgctctggt tgtcagttcg
     3961 tcaatcggaa gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc agctccaggg
     4021 ccagcgatcc tttcatggat aatcgttggg attggtattt tagctttggt attatcgctg
     4081 aatcatttag gagaaaagcg tccagatcta gatggtggta ttttcggtta cgcggaagct
     4141 tcatttggta aattaggtgg cttcatcagt ggttggggtt actggttgtc tgcgtggctg
     4201 ggaaatgttg cttttgcaac gatgctgatg agcgccatgg gagaattttt cccgatattc
     4261 aaaggtggac aaaatgttcc ttctatttta tttgcaagta tttttatttg gggtctgact
     4321 ttattagtaa acaatggagt tgaaggagcg agcgtcatca atacaatcgt gacgatctgt
     4381 aaattagtgc cattattcct atttttaata tttgtattta tcgcatttaa agtgaatata
     4441 ttcactgctg atttttgggg taacgtatct gataatctag tgaatggaac agggcagcaa
     4501 ggaacgatct ggttgcagat taaaggttgt ttgatggtta tgatgtgggt ctttgtaggg
     4561 atcgaaggcg caagtgtttt agccaaccgc gcgaagaaac gttcagaagc ccagcaagca
     4621 tcgatcattg gtctgctttg tttattgttt atctatatcc ttgcttcgat cttgccatac
     4681 ggcgtcttat ctcaagaaga gttagcaact atttcacaac ctgctatggc aaatatttta
     4741 aaaggaatcg tgggaaattg gggagcagcg ctgatcaata tcggcttgat tatttcgatc
     4801 attggttctt ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt gatggctcga
     4861 gacggtctgt tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc aacttattcg
     4921 ttagttgtta ctgcaatctt gactaatcta ttcttattga ctttcttagt tactgattat
     4981 gcctatcaat ttgcctattc tttatgtaca gcggcaattt tgatttgtta tctacttgtc
     5041 gggatctatc aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt agtgatcggt
     5101 gggattgctg tagtttttga attgatgggc atcattttag caggattttc ttatgtcctt
     5161 ttatgtagta ttgcctatct tcctggtttt tgcttctact ggatcgcttg tcgtgaaacc
     5221 aaacatcaaa ttacgggaaa agaaaaagta atgattgggc tgatttgtgt ggccgcactg
     5281 ctttcaatcg ggttgcttgc tgtaggctgg atcaagatat aagggggaaa gaaacatgaa
     5341 aatcgcaaat ttcggtgtag aagaatggct aaataaatgg gaaaagaaag cggtatatga
     5401 tattgcacaa agctcgatcg aagcattgac attagacgaa ctcgttggct tagatgggac
     5461 tagcgtaagt aaatttttcg aaaaacgcaa aaacgaacca ttggactatg gctggatcga
     5521 aggatcagat gttttcaaac aagaagtcgc ttctctttat caaaccgtcg atccggaaaa
     5581 catcttacaa acgaatggag cgactggtgc taatctttta gctctatacg cgttagtgga
     5641 agcaggagac catgttgttt cgatgcttcc tacttatcag caattatatg acatccccaa
     5701 atctttaggt gccgctgttg actttgttca tttgaaggaa gaagaagatt ggcagtttga
     5761 tttagaacag ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt tgaatagtgc
     5821 aaacaatcct acaggcacat tgctagatcg gaaaacttta gaaaaaatcg cagaaattgc
     5881 tcgtacagta gatgcatatg tattgataga tgaagtatat gcgcctttaa cggataaagg
     5941 tgaattcttg tcgatcgtag atgtctatga aaaaggtata gcaaccaatt ccctatccaa
     6001 aacctactcc attccaggta ttcgtattgg ctggacagct actggacctg agttggcaga
     6061 agtttttcga aagtatcgag actacacgat gatttgcggc ggagtcctgt cagatgatct
     6121 tgctgtacat gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa agatcattac
     6181 agagaacctt gtgattttaa aacaatgggt agccaatgaa ccgaaagttg aacttgtcgc
     6241 tcccaattac gtctctactt ctttcattaa attagccatc gaagaagatg accagacatt
     6301 ttgtatcaat ttattagaag aaacaggagt actattagta ccaggaagtg cctttgattt
     6361 accgaagcat gcccgactag gttactgttg taaaaaagaa accttggaaa agggattatt
     6421 cctcttatct gagttcttga aaaaacaggc ctaaacaaaa acactgcaag gacagtctgt
     6481 gacaactgtt caagcagtgt ttttcattta cgcttattgt ctatgagaag gagagtgccc
     6541 tgaagctatg gagactcctg aagctttttc ttctgcagtt tcttgctgat ggatctcttg
     6601 tgtattgtgt cgtacaagca agaagttgac caaaacaaga agagaagtcg aaagaaagac
     6661 gccacgataa ccaaatgcac tggaaacact ggagccgatc ataggcccta ctacgttacc
     6721 agttgcttgg aaagattggt tataactgaa gatccgtcca gcagcttgat gaggcgaata
     6781 ttttgtgatc aatgcttgga cagcagggag cagacaagca tcagagatac caatcaaaaa
     6841 acgtaaggca gctaattgcc aaacattcgt gacgaatgcc attgggatat aaaccagaat
     6901 cgcaaaaccc aaaccaatcg caagtattct ttcactccca atcttgtcac ctaagcgacc
     6961 aaatcttgga gcggctatca aagtggcaat cccaggtatc gaagcaatga ttccgctgat
     7021 caacgtaaca ttcccatgtc catgcagcaa ttggcgaatg tacagactga tgattggact
     7081 aatggaattg ttggatgctt ggatgatcat cgttgtgacg aacataccga tgacgacgtg
     7141 tggatatttc aagtctttga aaatctgttt ggcagaaacc atgttttctt ttttcactgg
     7201 gacaaatttt tcatggacga aaaacagact caacaagaag acgaaaaata agatgatccc
     7261 tgtaatgaaa aaggtagggc gatagccaaa cgctgaagca gagatcccgc caagaagcgg
     7321 tccaagtaat gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt gaccactttt
     7381 ttcttttggc gtcccagttg cgacaagggc agtggcatta ctgatataac cagaaaaaat
     7441 cccttgaagc agacgcaatg cgactaattg atacacacta gtcacgcaac ccatcaaaga
     7501 aatgacgatt gccattccta atgaagcacg caacagcatc aattttcttc ctttttggtc
     7561 ggctaatttt ccccaccaag gagagataag cgtagtcacc aagaaagtag atgaaaaagt
     7621 caatccgctc caaaagttca attgtgacgt actataatta cctaacgtat ctatgtaaag
     7681 tgacataaaa ggcatgacta aactaaatcc gataccagcc ataaatgttc caaaccataa
     7741 gacaaacaag ttttgttccc aagtttctct tttcctaaat aatttttcct ttattcttgc
     7801 agccaattag actccttctt tctgaaaatt ttcatctaca gctctttatt ataaactaca
     7861 atatgtagtt tatcaatgaa agtctctttt ttctctttct ttttcgtggt aaactgaaag
     7921 agtgaaggat ttattttatg agaggaaaca tggaaatgaa aaaaagaaat ctgtctcaag
     7981 agaaaatcat cgactgcttt cgagaactag cagaagagat ggatgttcag caaataacct
     8041 ttcagcattt agccaaacgc ttggatatca aggctccctc gctgtataac catttcaaaa
     8101 atattcgtga tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt aatgaccagc
     8161 tgcgacgggc tcttgtggga aaaagtggtc aggaagcact gcagatctat gcagaaactt
     8221 atcgatcctt tgctttttca aatcaagcag tgtatgagtt actgatcagt gtaccgcata
     8281 ccaatgaagc tgttttatta gatggaatcc atgaaacgaa tcagatcatt ttgcagatat
     8341 ttgaggcttt tcaattaacg aaagaagaaa aagttcatcg aagcagggaa cttcgtagta
     8401 tgatccacgg ttatctttcc ttacgttttt taggatactt tacaaaagaa ccaacaatcg
     8461 atccagaaga cagctatttt tggatgatca ataactttat cgctactttg ccttaaacaa
     8521 tcagattcat ttgtaacaat tttaagatgt tcattaaatg acccttttat aagagaaaat
     8581 tcttgcaact ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa gaaggagcac
     8641 gagtatgtat caagtaatta ccatgttcgg ggacaatgag ccttggtggt tttttgaaga
     8701 ctgggaagaa gatattgaag aagaagaaac attcgagtct ttcgaagaag cacgacaagc
     8761 gtacgaaaaa caatggaacg aaatccatca aaattatgaa tatatcaatg caaaaagtaa
     8821 ctttctcagc gccttttgga atgacggaga tgagcgctgg tgtgaagagt gtgacgatga
     8881 tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat cagcccatca cagtagaaag
     8941 cagaaaggaa ttttatgaaa caactaattc tagcggaaaa accaagcgtt gcgaaagacc
     9001 taagcaaggt gcttggtgct aatcaaaagc acaaaaatta ttatgaagga cccaaggtga
     9061 tcgttacttg ggcccttggt catttattag gattgaaaat gccggaagat ctgaataagg
     9121 aatggcagag ctggcaaatg gacacgttgc caatgatccc taaaaaacta ggaatcaaac
     9181 cacttcctaa aacaggacat caattaaaag caatcaagca gctggctcag cgaaaagatg
     9241 tctcagaagc agtcattgcg acagatgccg gaagagaagg ggaacttgtt gctcgctgga
     9301 tcttagagta tgtgcatttc aataaaccag tgaagcggtt atggatctct tctcaaacag
     9361 ataaagcaat caaaactgga ttcaagcaac taagacctgc gaaagaatat gatgcattgt
     9421 atgaatctgc tcttgcccgg gcaaaagcgg actggctggt aggattgaat gtgaccagag
     9481 ccttaactgt gaagtacgcg gataatttat cagcaggccg tgtgcagaca ccgacactag
     9541 cattagtgag agaccaagaa aagaaaatcg agcagttccg tccacaaacg tatttcacga
     9601 tcacgttgca agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa tttgctttaa
     9661 aaacacaaga agaagcacaa aaacttgttc aattgatcag tacaagcaaa ggaactgtca
     9721 cagaagtcca agaaaaatta aaaacggaat ctgcgccatt gccttatgat ttaacagaga
     9781 tccaaagaga agccaatcaa cgctatggct attctgcaaa gaaaacatta agtcttgtac
     9841 aaagtttgta tgagacccat aagatcgtta cttatccgcg tacagacagc aaatatctga
     9901 caaatgacat gaaaagtacg atgaaagaac gattgcaagc agtcagcgac tttgctcctg
     9961 aggtcaagca gtatttgaaa aacggagcca tcgtccaaca gacaaaagta ttccaagaca
    10021 gtaaagtgac cgatcaccat gcacttttgc caactgaaaa cagagcacgt tatgaaaaat
    10081 taagtaatga agagcgaaaa atctatcaaa tgattgtctc tcgtttttta ggactgtttg
    10141 ctcaacccca taaagtcagc caaacaaaag ttacggttga atttggtaaa gaacgattca
    10201 tttttcggca aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa actgagacgg
    10261 aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc aaaaaagaac
    10321 tatctgcacc gcctaaacca ttgtcagaag caagcttgct tggtcaaatg gaaaaataca
    10381 gtctgggaac cccagctacg cgtgcagaga ttatcgagaa actaatcaaa tctgaactga
    10441 tggaacgtac ccctcaagga ttgcaagtat ctcctaaagg taagcaatta ttagtattgg
    10501 taaatccctc tttagtaaca ccagatttga cagaaagctg ggaaaaagac cttgaagcta
    10561 ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa gaaacaaaac
    10621 gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca agatttctcg attacccaaa
    10681 agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac ggcaaaatct
    10741 atgtctgcac caattcagat tgcaactacc gcagaagaaa agatccaaaa gtctctaatc
    10801 atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac ggtgcttatt
    10861 tccgttgtaa atacgatgga acgacagaaa aattgcttga taaaaaagag cagaagaaga
    10921 aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca gaagaaccag
    10981 aagaaagccc attagcagca gcactaaaag cagcaatgaa gaagtaataa gatatgaaag
    11041 aggttgtgat agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct
    11101 caaatttttg tgaaatttcg gcttatttcc gaagaagcta cttctggaac accatttatt
    11161 cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa aaaaatgttt
    11221 acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac tttttctgtt
    11281 tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc aatatatttc
    11341 ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat cgagaccatg
    11401 gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat gaacaaaatt
    11461 gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt taacggtgca
    11521 tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa ggaccaagat
    11581 gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt aaatccgacc
    11641 cagctaaata atccttttga aaatcggttg tattctgtta attctaggac ggcatccacc
    11701 atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt atcgctgatt
    11761 ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg ctcgccttca
    11821 cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg gttgatcatc
    11881 tcaggaagca gttcgggcgg atcttgcaga tcaacatcca tcacggtcac taaatctcct
    11941 gtagaagctt gcaatccagc gtacagagcc gcttctttcc caaaatttct ggaaaatgag
    12001 acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa cgtaccgtct
    12061 tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa tttttcaacc
    12121 tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac aattgatact
    12181 ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca aaatttaata
    12241 aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat tctttttgta
    12301 aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag aacaattttt
    12361 attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct attgactagg
    12421 agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg cagatgaatc
    12481 cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga atcagcgaat
    12541 tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa aagaatttga
    12601 tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga aagctttgtc
    12661 gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg ttgatagtcc
    12721 gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa tcgttattcc
    12781 ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa acgcaataga
    12841 tggggttgaa atcaaacgcc aattgttgtt gttagaagga tatctgcaaa a
//
LOCUS       DAXWLH010000072        12205 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXWLH010000072 DAXWLH010000000
VERSION     DAXWLH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.072"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..286)
                     /locus_tag="ACGE36_001574"
     CDS             complement(<1..286)
                     /locus_tag="ACGE36_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8383968.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
     gene            938..1909
                     /locus_tag="ACGE36_001575"
     CDS             938..1909
                     /locus_tag="ACGE36_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFF8383969.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1808..2047)
                     /locus_tag="ACGE36_001576"
     CDS             complement(1808..2047)
                     /locus_tag="ACGE36_001576"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8383970.1"
                     /translation="MQNTKAQADLLPRLLLPQNVKQAYLELGGLPELDGRYTVFGQVY
                     QGLEIVAAIAAQPTNSEDVPIEPVFIRQINFEKTS"
     gene            complement(2022..2246)
                     /locus_tag="ACGE36_001577"
     CDS             complement(2022..2246)
                     /locus_tag="ACGE36_001577"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8383971.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFAGEVHRGLY
                     HFNGALALGDHGPHTNGNQFFYCAKHKGAG"
     gene            complement(2215..2346)
                     /locus_tag="ACGE36_001578"
     CDS             complement(2215..2346)
                     /locus_tag="ACGE36_001578"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8383972.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2422..2805)
                     /locus_tag="ACGE36_001579"
     CDS             complement(2422..2805)
                     /locus_tag="ACGE36_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8383973.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2838..3623)
                     /locus_tag="ACGE36_001580"
     CDS             complement(2838..3623)
                     /locus_tag="ACGE36_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFF8383974.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3801..4901
                     /locus_tag="ACGE36_001581"
     CDS             3801..4901
                     /locus_tag="ACGE36_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8383975.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4891..5715
                     /locus_tag="ACGE36_001582"
     CDS             4891..5715
                     /locus_tag="ACGE36_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFF8383976.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5712..6527
                     /locus_tag="ACGE36_001583"
     CDS             5712..6527
                     /locus_tag="ACGE36_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383977.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6524..7354
                     /locus_tag="ACGE36_001584"
     CDS             6524..7354
                     /locus_tag="ACGE36_001584"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8383978.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7361..8392
                     /locus_tag="ACGE36_001585"
     CDS             7361..8392
                     /locus_tag="ACGE36_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8383979.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8499..9772)
                     /locus_tag="ACGE36_001586"
                     /pseudo
     CDS             complement(8499..9772)
                     /locus_tag="ACGE36_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9999..10184
                     /locus_tag="ACGE36_001587"
     CDS             9999..10184
                     /locus_tag="ACGE36_001587"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFF8383980.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            10209..10928
                     /locus_tag="ACGE36_001588"
                     /pseudo
     CDS             10209..10928
                     /locus_tag="ACGE36_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(10926..11446)
                     /locus_tag="ACGE36_001589"
                     /pseudo
     CDS             complement(10926..11446)
                     /locus_tag="ACGE36_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            11734..>12205
                     /locus_tag="ACGE36_001590"
     CDS             11734..>12205
                     /locus_tag="ACGE36_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8383981.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKC"
BASE COUNT         3512 a         2270 c         2776 g         3647 t
ORIGIN      
        1 agtagcctaa aaaccagcgg taagcgacat tggtatcgac ttccttaatg gtttgtcgca
       61 tggaacggat accgaataaa tactgaataa acaccaattt gatcagaatc actggatcga
      121 tacttgggcg acctaaatcg gagtaagtgg cttcgacgat tggataaata aattcaaaat
      181 caagcgcggc atcaatcttt cgaacgagat gatcttgagg aactagatca tcaatcgtaa
      241 gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga catcatagct atcactcctt
      301 gcattcgttt tcataaatct attataaaag aaaagactgt agacatcatc cataaaatgg
      361 actttgtcta cagtctggat aacgtgattt tttgctgtaa tgacaaggtt ttagctgttt
      421 cagataccat gtatccaaaa atagtttatt gagaaaatag gagagtattt gatactaact
      481 agtaggaaaa aatgagataa aaagtaaatg gaatcattta aggtaatctg aaataaaaaa
      541 tatacagccc tgtggtactt tacctacaag gctgtatata ttttttactc tcagcgtttt
      601 tgactccgtg tctgactccg ttttattgat tttgaatgtt attcaatgac catttaacag
      661 gtcaaatttg atgtttttga ttccgtatga cgccgtttga tatctagtga tatagtgatt
      721 tgagtctaca tcgaataagg gaataaacca ataaaaaatg atttagaata ttaaaataac
      781 aaaaaagagg actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt
      841 acatcctagt atatttatat gagctaattt tatgattaaa tggtaagttt ttccttgtgt
      901 gttttagttt aaactagtgt gtaattcaaa aatctatatg gaaggaacag tattaagaat
      961 agaacagggt tctctacatg atggtgctgg tttacggacc gttgtttatt taaagggctg
     1021 cccattacgc tgtgcctggt gttcgattcc cgaatcacaa agcaagcaga ttgaaaaagg
     1081 atttggccag accatgaccg ctgaagaggt aatggatgag attgagaagg atgctgtttt
     1141 ttactttcac tcggatggcg gagtgaccat tagcggaggc gaggcactgg ttcaggcgga
     1201 ttttgctaaa gaaattttgc aaaaatcgaa atacattgga atcaataccg tactggaaac
     1261 cagcttttgt ggtgcttata atgaaatcca aaaggtagct ccctatgtgg atacattgtt
     1321 tgtggatgta aaaatgttta ccagcaagct ccacaaacaa tggaccggct tagataacca
     1381 acaaattttg aagaatattc gtcgcttttt aattgagtat cctaactgcg aagtgcgaat
     1441 tcgagttcct gtggttccag gtatcaatat gaacctgacc gagctgctga cgattgcttg
     1501 ttttgtggca gatcttgatc ggttcgtacc gttagaatta ctaccgtacc actgctatgg
     1561 tatgcacggt taccaagcat tgggactgga gtatccgttg gcagacacgc cagcgccttc
     1621 acctaaagaa atgtttgcat tggcagatca actggctcgc acggttcctc atttgctggt
     1681 cacagcttta accaaaacat ttatttattt attgaatcca agctttatct tatggataaa
     1741 tgaaagacac tcactagctg cagccagctc aagaatagag cttttgctga agtcagtgag
     1801 tgtcttttta cgatgttttt tcgaagttaa tttgtcgaat gaacactggc tcaatcggta
     1861 catcttcact gtttgtcggc tgagctgcga tcgctgctac gatttctaac ccttggtata
     1921 cctgtccaaa aacagtataa cgaccatcca gctccggtag tcctccaagc tccaagtatg
     1981 cttgctttac attctgagga agcaagagcc tcggcagcag gtcagcctgc gcctttgtgt
     2041 tttgcacaat aaaaaaactg gttgccgttg gtgtgggggc cgtggtcccc taaggccaag
     2101 gcgccattaa aatgataaag tccgcgatgc acttcaccag cgaaaaagcc ctcatagctg
     2161 gaaacagctt catcaccaga accatccaaa gcgccacctt gaataacaaa ttccttaatg
     2221 acacgagccg aaaggtgtgt tgtcataaac gccttgctca gctagggagc gccagttggc
     2281 tacagctagg ggagcttggt caggaaataa gcggagacga atttcaccag catcggtgac
     2341 cagcacacaa acttcttcct gctgcggttt ttctaattga aagagaggaa ggtttgcaac
     2401 tgaaacgcct tgatcctgcc atcaaatatc gaaaatccgc cactcgacct tcgcctgatc
     2461 accaaggcga gccattccca ataaaacggt ataggcggtt tcatccgctt gaaaggtcaa
     2521 ttgcaactgt cctcgtaaat cgttgtagtc atctggggcg gctacttgat aaaaaagatg
     2581 aaactgcgca tgcaaatatt ccaaaggctg atctttaaag gctgcaaaat aagcctgaac
     2641 tttttcccgc tgcaagctgt cagccaccaa aaaattttcc agttgttgtt ttggaacggc
     2701 tgctaacaag gatcggcaaa ccgcctcaat tttcaacgca tcccaatgtc gcgcaattaa
     2761 ataatggatg ggtgcctctt gattgtagtc ccacaggttg ctcatttttt tcaccacttt
     2821 cttctattat ataggaatca caaaatcggc agcggcgtca ggcggcgttc ttttccttga
     2881 atgactagca acttttctac ctgacagtct gccaacagct gactcatttt tttaaagccc
     2941 agctgataat cttcggcaac atgagcatcc gagcctaagg taaagagctt tccgccgaca
     3001 ctttgataca aaggaacggc ataacggtaa aggtcttcgt tgccgtattt caagaagctc
     3061 ttggcattta actccatcgc caattctcgt tcaacaacct ttttgaaaat cgttagcagc
     3121 tgagcctcaa aatgctccgc gaattcttcc gctgtgaagg tgaagcggcg taatccataa
     3181 tcaaagtgag tcaaaatatc tccttctgga aaatccgtca agacctgcaa catctgatca
     3241 aaatattcct tggtagtttg aatgggatct ttggttaaaa ccacatcgtc catataatca
     3301 aagcggccat tttgatgaat acttaccagc tttaaatcat aaggatgctg agcgaggtaa
     3361 tccacgatcc gctcttcttg tcctggaaca acgccaatct cgatacctcg cagaaatttg
     3421 gtggaatagg tttcttttaa agagtccagt ttgtttatat aggctggata atccggaatg
     3481 tcatcttttc cggtagatgg attgtttaaa tcaaagtgat ccgtagaaac gaaaaactcc
     3541 ggctgatagg ctaaatagtt ttcaaatttt tcctctgaat caaaggaaaa atacgtgtgg
     3601 agatgctgat cataatactg catagggagc ctcctttttt ctactatcat accgcaaaag
     3661 ctttagaagg ttaacgggaa ttcgtcgaaa tttacaaacc atatacaacc tttacattgt
     3721 tcatgaaacc ttttcaagaa actgattctc tcattacaaa gctttgttag aatggaatta
     3781 tcgaaagggg agaagaatga atgaaaatga atgaaaaaat tcgtaacatg actatcggtt
     3841 tattttgtgc agcaacagta agtatcctgc tgacaggctg cggctcaggt gaagcaacga
     3901 agcaatcggc tgcggcagac gactctttag atacgattat agaaaaagcc aaggaagaag
     3961 gagagattgc cagcttggga atgccggata cttgggcaaa ttgggtaggc acttggaacg
     4021 accttgaaaa agaatacggc ttaagtcacg tggatacgga tatgtccagt gcagaagaat
     4081 tggctaagtt tgaatcagaa ggaaaaaatg gaaccgccga tattggtgat gtcggtatca
     4141 gcttcggtcc gttggctgaa agcaaagagt taaccttacc ttataagacc aactattggg
     4201 aggaaattcc ggattgggca aaggatgatg atggggattg gttgttaagc tatacgggaa
     4261 ccatttcctt tatcaccgat aaagagaatg tgaagcaagc accgactagc tggaaggagc
     4321 tgcaagaggg tgactatcaa gttagcgtgg gggatgtgac gattgccaac caagcacaat
     4381 ttgctgtatt ggctgcggcg attgccaacg gaggtgacga aaaggatatt cagccaggtc
     4441 tgaattacta caaaaaaatg gcagaaaaag gccgtttgtc caccgttgat gccagcgtag
     4501 caaatcttga aaaaggtgaa atcgatgtgg cagttgtttg ggacttcaat ggactaaatt
     4561 atcgtgacca aattgactcc gagcgttttg aggtaactgt gccaacagac ggctccatca
     4621 ttagcgggta tacaacctta atcaataaaa atgcccctca tcccaatgcg gcaaagctag
     4681 cacgggaata tattttatct gatgaagggc aaatcaattt ggcaaagggc tatgcgcgac
     4741 ctattcgcaa ggtagagctg cctaaagaag tagctgaaaa attactgccg gaggatgctt
     4801 atgaagcggc ggtgccagtt gaagacaatg aagcttggaa caagacctct gtagagcttc
     4861 cagagctttg gcaggcggag gtactgacct atgcaaagta gacttgacaa actgattgta
     4921 gttatactag acggctgtcg ctacgacaca gccgtcgccc agcttgggtt catgaatcat
     4981 ttagttgagc accagcaagc aagcttattg aaggtaaaaa gtgagctgcc cagcaattca
     5041 cgtccgttat acgaagtact gatgaccggc gttccagcct atgaaaatgg catctatagt
     5101 aattaccatg ctcagcgatc caaagagtgg tcgctgtttg aattagttaa ggaagctggc
     5161 ggaaaaacag gagccgccgc ttaccactgg tacagcgagt tgtacaatca agccccctat
     5221 aatcgtttgg ttgatcgcat ccagcatcag gaggagcgac tgattcagca tggcatcttt
     5281 tatagtgagg acacttaccc tgattcgcat ttatttgcgg atgcccatta tttagtgaat
     5341 cagtatcagc cagacttttt ggtcgtacat tcgatgaata ttgatgatgt cggtcacaaa
     5401 tttaccgccg attctcatga atatgcagca gcggttaatc aggcggacag attattggct
     5461 atttaccttc ctgaatggct gcaaaaaggt tatcaagtcg tggtgactgc ggatcacggg
     5521 atggatgcgc acggactgca tggcggcagc ttggctgcac atcgtgaagt tcctttttat
     5581 ttgatttcaa ataaacgcca aaagctgaag gaacaaactg taccacagct gctggcggct
     5641 ccattgtttt gctggttatt aggaattgca ccgtcagaga aaatgcagga cattcaagaa
     5701 ttgatgaggg gatgaaagag atgaaaaaac gcttggttct ggttcctgcc tcctttatct
     5761 tctgtgcatt tttactgctg ccgctgcttt ttatggttct gtgcagcttg cacagtgatt
     5821 taactaacca gtggacattg gccaattaca gtgatatttt cagcaacaaa tattacacac
     5881 aggcctttgt taacagcgta ctgattgctg tcagctccag tgtgattggg ttaatcggga
     5941 cgtttatttt atgtttatgc ttaatgaatc tatcgactaa attgcaggag aaaatcacct
     6001 ttgtttccaa tttggcagct aattttgctg ggattccgct ggcattctct tttattattt
     6061 tgttggggaa tgtaggtgtg ctgaaaatga ttttgccatt tctagcggac ttcaatttgt
     6121 attcttggtg gggattgacc atcacgtata cctatttcca aattccgtta ggggtcttgt
     6181 ttttgtatcc cagcatgaag gaagtgaaaa aggaatggct ggagtccgca caattgctgg
     6241 gcgcttcatc agcgtatgct tggttcaagg tggctttgcc ttttttgcga ccatcgattg
     6301 ccagcacctt tgtcatctta tttgctaatg gcatggggac ctatgaaaca gcgtatgccc
     6361 tgaccagcag taatgtaaat cttttaacga ttcgaattgc ggctttagtg tcgggagatg
     6421 tgttcgccaa gcctaattta ggcagtgcgt tggcagtcgc tttaggtgtc ctgctggtaa
     6481 cggcaatgtt gattattcaa aagaaaggca aggtggtgca gacgtgaaaa agttaataaa
     6541 accgattttg ttctgctttg tcctgtatta cttgctgccg attattggaa cgattttgta
     6601 tgccagctcc actaaatggt caaaatcgct tttacctagt gattttacct tgcaatggtt
     6661 ccaacagctg ctgacggaca gggaatttat tgctgcggtg ggtcgctcac ttttattagc
     6721 aggtgtggtc ttggtgacga ttctgttgtt gatgattccc accattattt ggattcattt
     6781 gtatttccct cgtttgaatc gttggctgga aaagctgctc ctgctgccgt atgctttacc
     6841 aggagtgatt ttggtaacgg ctttgcttcg aacgtatgct gaaactggta tcccaatgtt
     6901 tgtagtgttg gtaggtgcat tgtttattac ggcgttgccg attgtctatc ttagtcttaa
     6961 caaccagatg cgcttaatta atctgaagga attagtggat gcagcagaaa ccttgggcgc
     7021 cccgatgtct acgattatta tccaggtgct gtttccaaac atccgtatcg gtgtgaccct
     7081 agtttctctg atgatttttt cttcggtttt tggggagtac atgctgacga acctgttgat
     7141 tggcggtcga tttcagacgg tacgcatcta tatgctgcgg cggatgaacg aaaacggcca
     7201 tttggctagc gctgtgatgg ttttgtattt gctgtttatg gtgggaattg ctttaactac
     7261 ctttatgctg acttatcgac aaaagcaaca gctgtccctt aagcagacga gggaagagaa
     7321 gcagtcgaaa aagaagccct taaaggagtt gtgacggaaa atgtttttag aaatacatga
     7381 tctcaatctg ttttttgatg aaaaacagat tttagctatt gatgaaattc agattgaaaa
     7441 gggtgagctg gttactttac taggtcccag cgggtgcgga aagtctacgt tgctgcgctg
     7501 tatcacggga ttggaacaac caaagagcgg tgagattgtg ctggacaatg agaatattgc
     7561 cgataagcct acgaaggatc gcaatatcgg ctttgttttt caacagtatg cattgtttcc
     7621 aactatgact gtttttgaaa atgtagcctt tggtttgaag gtaaagaagc tgagtcctga
     7681 ggtgattcag gaaaaggttt ttgagatgct atcattggtg gatatgacgg agcaggcaga
     7741 taaaaatgtc cagtttcttt ctggcggtca acgacaacgg gtggcactgg ctcgctcatt
     7801 ggtcactgaa ccgaaagtcc tgctgctgga tgagccatta agtgctttgg atgcccgaat
     7861 tagaaaacag ctgcagcggg atttgcgcgc tatccagcaa tcgctaggga tgacgatgat
     7921 ttttgtgact catgaccaag aagaggcgat gcgaatcagt gatcggattt ttgtgatgga
     7981 agctgggcgg gtggctcagg tttccacctc gaaggaattg tatcagaatc ctcagagccg
     8041 atttgttgcg gaatttatcg gcaactataa tcgtttcgaa tggtatgagc tgaatcagta
     8101 tacccacgag ttttccgcta agggcaatcg ctgcatttat tatttaagac cggagctgat
     8161 tcaatttgaa ccggttcctc agggggttaa gattccggtt ctatggaagg aatcatttat
     8221 tttaggcaac attcaacggt atgtttttca aacgctggag ggcaaagaaa ttttagtgga
     8281 tcggttgaac gatcgggaag agaaaattag cgatgcctta tacattcagc tgaaagatat
     8341 tctagcaatc cctgtggaaa ttgatactca gcattttgca caacaaagct agtgtatagt
     8401 cggcaaacat ttctggctct ttgtcaaata ggactgatag agtgatataa gaacatctaa
     8461 ggtagaattg attttctgct ttagatgttt ttttatgttt aataagtgat cagatgattg
     8521 attaagaaaa ttctggttct catcctaacc cgtaagctat tcgtttcaat gctttgatgt
     8581 gggtatgcag attttctatt ttgccatttg agtaaggcaa ctctaaggct aaacgaatgc
     8641 cggattcata gtttataaac gcttttttta tatgatgcag ttctcttgga accgacttgg
     8701 gcattgtttt tacaagctga aagaagatgt ccgcttcttt gtcataaaaa gcttgaagaa
     8761 tttcttgata gtaagcataa gctattcgta gttcatcaga agtagaaagt agacgttcta
     8821 cgacttcact ttcggttaaa taggcccaac caaaattacg ccattttgtc catttatcat
     8881 aaacaagcgt gtcctgtcgt ttgatcagaa gtcgagataa agccttcact tgtcgatagt
     8941 gtttggcttg tattggatcg tttcggtcga agcttttcat gactcatagt caaatatcct
     9001 taaaactgcg aatgaggtgc ttgacgatgt gaaagcgatc cacaacgagt ttggcatgag
     9061 gaaagcactc ttttatcagc aaagcataag aggcgttcat atctgttacg agtattttta
     9121 cgtttaaacg tgcacgtcgt tcaaattgca taaaataaga aatgagatct ttcttcttac
     9181 gtgaaggaag aatatccagt aatttcccag tatcgccatc catacaagaa aaagaatagg
     9241 gaccgacttg atttttcaat gaacgaaatt catctaccaa tgaacagcga ggcaaccaat
     9301 ttattttcac gtttgtcatg gggagcagag aagcgagtgt tcgcgaaacg gttgaccaag
     9361 aaacgccgca atgtttagaa atcgtcgtca ttgcttgttt ttcagtcaat tcctccaata
     9421 ttttataatg tacttgtttg gcgataaaac agtttttttc aacaatgtag gtattcgccg
     9481 taaaataggt gtgacagcca cgacagaggt accgttgctt tgccaaagca agataagtcc
     9541 gttgattcgc acacttattg agaaggacca gcgatttttt ctttccgttc ttaacgatca
     9601 gcccatgttg attcacctgt ttacactgag gacagggctg aatcgtttta atgagcgtgg
     9661 cagaaacaac gatactgttg attgaatgta tttttttctt ctcgacatga gtgatttgaa
     9721 tgtttccatc tttgattcct agtaatagtt tgataagatg aagatcaggc atacagaacg
     9781 ctcctttgat tgtgttgtgg tgattcaatt ctaacggagc ttctgtatgt tttgtttttt
     9841 tatgctcaaa aggactgata gaatgattta ctatcagtcc tagaaattat agcgccacat
     9901 ttctttcacc tttgactttt cagacggcag tttttatgct gatactgtag agagagcagg
     9961 acacagcttt tgcttgtttt gggtaagggg gaaagaagat gaccaaagca cttatttttg
    10021 attttgatgg gacgatgatt gacagtgaat ggatgtttta cgagagtatt catcactatt
    10081 tactgaagcc tatttactgg agcagttcgg ctataaaatt ccttttgcag actatcaaca
    10141 aggtgttggg acggcagcag aactgtcctt ttatgaaggg ataagtcaag cggttgggag
    10201 accagtggat gctgaacatt ttgagcagcg gatcagcagc actggaaaat cggagtagtc
    10261 tcaattctga actgcgggag ctagactatt tttttgaagc acatccgatg attaaggaaa
    10321 aaattgattc ctttgtgacg atcgatgatc ttcaggtcgg caaaccagat ccagaaggtt
    10381 atttgttgtg cttgaaacgg ctgagggttg aacaggctgt tgtagttgag gatagtccag
    10441 tcggtgccca agcagcaccg catatgacag ttgatgtaca agcgttggat gcagattttt
    10501 tcgcctttag cgggcataaa atgtgcgggc cgacaggaat cggtgtatta tatggcaaac
    10561 gtcaattgtt agaacaaatg gagccagtcg aattcggcgg tgaaatgata gattttgtca
    10621 atctttacga tagttcatgg aaagagctgc catggaaatt tgaagctggt actccgaaca
    10681 ttgcaggagc aatcgattac ttgaatgaga ttggtatgga gaatattcat cgttatgagc
    10741 aagaactggt tgcctacgtt ttacctaaat tacaagcgat cggcggtatc accatttacg
    10801 gaccgcaaga tcctgaacat catactggcg tggttgcttt taatttggat ggcatccatc
    10861 cccgtgatgt ggcgactgct ctggatatgg aaggtgtcgc tgtacgttca ggacaccatt
    10921 gtgcacaacg gttcatcgac gatttctgtt tgactaattg gtcgtttaaa cagtcgatga
    10981 taatgccgtt tgagcggctc caatgtcgtg gaatctttga gtaagagttt ccaatatcgc
    11041 ttgagccgac ggtacttctt ctgattttca gattacgtga ttgattttcg tacagttcta
    11101 tgagaatcgc ctgcttcagt tcctttgtta gttgatgatg ttcatcaact aagtccgttt
    11161 tcgcagaaaa ggtctgccca caatcatagc atagaaaacg ggtacgtttt aattctaaaa
    11221 tagtcaactt ttctttgact ttcaacatct gagtacgtgt cgtataggaa ccgttgcgga
    11281 taatacgaat cgatccacag tgttcacaat gggaaggtga gtcatccaat ctccctttaa
    11341 taaactgcgc ttgaacgcct ttatattctt tttcaatcag ccattcttca tcaaaaaata
    11401 aatgtttatc tgttaatccg agtaattttc tagtatgatt atccatagag cgactccttt
    11461 ggtagggtga tgttttggtc gactaaattt taccaaaagt gagtctcttt ttgagttgaa
    11521 acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caacaacaca aaatattata
    11581 gaagcctttt ttctaaagga tttcaaggtg tctgtttgca tttattaaaa aaaagaaaaa
    11641 gtattggcaa cagaaggcgt caccaacact agatattata gagccataat atgctgtata
    11701 atatctagtg tataggctta agtggaggta tatatgaaaa tattaactgt tgaagatgat
    11761 aatatgataa gagagggaat aagtgaatat ctttcagaat ttggatatac tgttattcaa
    11821 gctaaagatg gaagagaagc cttgtcaaaa tttaatagcg atataaattt ggttatctta
    11881 gacattcaga taccttttat aaatggttta gaagtgttga aagaaattag aaagaaaagc
    11941 aatttaccaa ttctaatctt gactgcattt agtgatgaag aatataaaat tgatgcattt
    12001 actaatttag tagacggata tgtagaaaag ccattttcat tgccggtctt aaaagctaga
    12061 atagattctt taattaagaa gaattatgga catttagaga agtttgaata taaggatcta
    12121 tcggttaatt ttaatagcta tacagctaaa ataaatggtg aagaaataga tgtaaatgca
    12181 aaagaactag aagtattaaa atgtt
//
LOCUS       DAXWLH010000073        12147 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXWLH010000073 DAXWLH010000000
VERSION     DAXWLH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.073"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..102
                     /locus_tag="ACGE36_001591"
     CDS             <1..102
                     /locus_tag="ACGE36_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HFF8383982.1"
                     /translation="LRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            147..1172
                     /locus_tag="ACGE36_001592"
     CDS             147..1172
                     /locus_tag="ACGE36_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8383983.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            1169..3352
                     /locus_tag="ACGE36_001593"
     CDS             1169..3352
                     /locus_tag="ACGE36_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFF8383984.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            3352..4480
                     /locus_tag="ACGE36_001594"
                     /pseudo
     CDS             3352..4480
                     /locus_tag="ACGE36_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            4477..5154
                     /gene="pgmB"
                     /locus_tag="ACGE36_001595"
     CDS             4477..5154
                     /gene="pgmB"
                     /locus_tag="ACGE36_001595"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8383985.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            5238..5765
                     /gene="msrA"
                     /locus_tag="ACGE36_001596"
     CDS             5238..5765
                     /gene="msrA"
                     /locus_tag="ACGE36_001596"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8383986.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5966..6652)
                     /locus_tag="ACGE36_001597"
     CDS             complement(5966..6652)
                     /locus_tag="ACGE36_001597"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFF8383987.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            6732..8270
                     /locus_tag="ACGE36_001598"
     CDS             6732..8270
                     /locus_tag="ACGE36_001598"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFF8383988.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8390..8857
                     /locus_tag="ACGE36_001599"
     CDS             8390..8857
                     /locus_tag="ACGE36_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFF8383989.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            9391..9819
                     /gene="rplM"
                     /locus_tag="ACGE36_001600"
     CDS             9391..9819
                     /gene="rplM"
                     /locus_tag="ACGE36_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFF8383990.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            9835..10227
                     /gene="rpsI"
                     /locus_tag="ACGE36_001601"
     CDS             9835..10227
                     /gene="rpsI"
                     /locus_tag="ACGE36_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8383991.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10315..11541)
                     /locus_tag="ACGE36_001602"
     CDS             complement(10315..11541)
                     /locus_tag="ACGE36_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8383992.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTSDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(11613..11915)
                     /locus_tag="ACGE36_001603"
     CDS             complement(11613..11915)
                     /locus_tag="ACGE36_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8383993.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         3951 a         2258 c         2529 g         3409 t
ORIGIN      
        1 ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt gaaaaactgt
       61 ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa taacaataag
      121 taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc gctggacaag
      181 tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta gaattggccg
      241 ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca ggattttctc
      301 atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc gtgagtgtct
      361 gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct aatgcttcag
      421 tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg gcagagacag
      481 ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct gacgaagcaa
      541 caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa gcgaatgcgc
      601 ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt caaggaggcg
      661 ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt actggatttc
      721 ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa aaaaattcag
      781 ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt tttggcacac
      841 tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg aatatcagag
      901 aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg tatccaggcg
      961 aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg ccagttaatg
     1021 acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac cagactcaga
     1081 tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat ttatatcgtt
     1141 cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt ttgatttaga
     1201 gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg gtatcagagg
     1261 aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg gtgtatataa
     1321 ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca tcagatggga
     1381 aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg catacgagcg
     1441 ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa ctgctcaagg
     1501 aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc atcaggcagc
     1561 cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga aaacgggtat
     1621 cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac tgcgagtatt
     1681 tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg atattgggat
     1741 tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga tcaccgcttc
     1801 cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca tcgtgctctc
     1861 tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc agaaaagcgt
     1921 tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt ggcatcagca
     1981 tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact tcgctgctta
     2041 ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg cgaagggact
     2101 aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta tcctgccttt
     2161 ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt atgatcgatt
     2221 agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt tcccatggga
     2281 aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata tcaaaaccgg
     2341 aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag accttgctta
     2401 tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa caaagggaaa
     2461 aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag tcgatgatca
     2521 tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg acaataatgc
     2581 atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc aagctggcga
     2641 tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc cagtgcctaa
     2701 caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta ttgatttgac
     2761 cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc gtagcgaagt
     2821 aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact tattacctga
     2881 gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca aaacaatcca
     2941 cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc aagaaatgga
     3001 atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag atccgcattc
     3061 atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag ttatatttgg
     3121 tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac ttccttcagc
     3181 atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat ttgttttgac
     3241 aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt ttgtcaatgg
     3301 agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat aatgcgtatt
     3361 ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc acaagccatt
     3421 caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc tataggcgat
     3481 ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg gcaaacagca
     3541 gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga cggtcaaact
     3601 gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa ccaacaacag
     3661 ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt gcaacaaggt
     3721 gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg catcggcatg
     3781 gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga agcactggga
     3841 gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga gctgattaaa
     3901 gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga aggcgcttct
     3961 tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc tgaccaagcc
     4021 atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg cagtagtgct
     4081 ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt ctctggcatc
     4141 gactacgtat tggatttact ggagttcgat acgggtacag caagcagacc tcgtaatcgt
     4201 aggcgaagga cgtctagatg gacaaagtct aaaaggaaaa gcgccagttg ggattgctag
     4261 aagagtacca gagggcgttc ctgtgatagc tatttgcgga agtgttggta aaggaagcga
     4321 aaaagcagca gatgttggca tttcggccgt ttttccgatc cttcctgcag ctgtgccttt
     4381 acaagaagct ttacagcaag ccactgataa tcttcatcgc acagctaaaa acatcgcatc
     4441 tttacttcaa gtcgttcatt ttcaaggagg taatcgatga aaatcaaagc agcactattc
     4501 gatttagatg gcgttcttgt agacacagct cgttatcatt acgaagcgtg gctggtttta
     4561 gctaaccagc tgtccattcc attcactgag aaagaaaacg agcaactgaa aggaatatcc
     4621 agaaccgagt cactagagcg attgctgtca tttggaaaaa tggaacagaa attctcagaa
     4681 aaagagaaat cagcctttgc tgagcaaaaa aacaatctat atcttcaagc gattcaaaaa
     4741 atggatgaaa catccgtttt gcctggtgca atagctgttc ttgagtactt gaaaaaaaca
     4801 aacatcaaga tcggattggg atcagcaagc aaaaatgcac gcctcatttt agaaaaaacg
     4861 aatttgacaa gttattttga tgtccttatt gacggcacgc aagtatcaaa agcaaaacct
     4921 gatccagaag tttttctcaa aggagcacag caattgaatg taccgccaaa tgcatgcttg
     4981 gtcattgaag attcagaagc tggttgtcaa gcagctttgg ctggtaacat gcatgtattg
     5041 ggaataggtg aaaacataaa tcttccttct gctgaatatg tgattcctga tttaaccgtt
     5101 tttgatcaag tacgttcgtt ttggcattta tcagaagccg tatcacgtat ataatagttt
     5161 ccgttttttc tgtcacaagc aaaacgttat cctccttttg gaaatcaagg tatgatgaga
     5221 aaaaaggggg attttatatg acaaaaaaag cagttttcgc aggcggatgc ttttggtgta
     5281 tggtcaaacc atttgaagag cagccaggga tcatttctgt gacatccggt tatactggcg
     5341 ggcatgtacc aaatccaact tatgaacaag taacaacagg cacaacaggg catacagaag
     5401 cagtagagat cgaatatgat ccagaagtta tttcttatga tcagttggtg gaaatctatt
     5461 ggcagcaaac cgatccgact gacgcattag gacagttcgc tgatcgcggt gattcttatc
     5521 gtccagtgat ctactataat gatgaagaag aacgaaatat tgcagagcgt tcaaaacaag
     5581 cattgcaaga cagcgggcga ttcgataagc caatcgtgac aaaaatcgaa ccaagaagcg
     5641 aattttatcc tgcagaagat taccatcagg attattacaa gaaaaacaag ctccattaca
     5701 atctttatcg agaaggttct ggacgtgcag gtttccttcg tcgacattgg ggcgtcaaga
     5761 aataagaagc cgaaaaagct acttatggaa caccgtatat cagaggttgt aaaaccagca
     5821 ttttgagcta atactgaggg tcagctgatt gaacaccatt tagtggagag gttgtgaacc
     5881 tgtcgcctag ctccaagtca caaggatttg gtaagagaac actatatatc agaggttgtg
     5941 acaagtcgtt tgtcacaacc ttttttcaat acctaagatg aaggacaatc agcaacgcac
     6001 tgctcaaaaa aggaatgaaa ggcagctttt ccaacttttt tttatgacag ataaagtaaa
     6061 cagaaaaaac ggatattcca aacagactag caagaaacaa gatctgaagc aactcttcta
     6121 gagacaagcc accactccaa gaaagcatca acagagtatc gccagctccg agataggatt
     6181 gaccatagcg aaggcacaca ccgcctacga taaacgttaa cagcacactt tgccattgga
     6241 agacgccggc ctgcaaccaa aaaatccata aaattgccca agaacagaat agcgtgtgag
     6301 aatggaccat gaagtaaaat aaatccatca acgaaaatag caaagccgaa agcatccata
     6361 tgaacgtcga aaaatttctt gcggaagaaa aagtaaagaa ccaggcaaaa agaccaccac
     6421 ataaggcttc tgccaacaaa tagtagaagg gcaaccgaca cctgcaatag cggcagcgaa
     6481 atcgttggac gatgatcgag agaatcggaa tcaattcata ccaagaaagc gaacgacagc
     6541 atttgacaca atgagatcta ggataaataa tagagtgacc taacggaatc cgctgggcgg
     6601 ttaaacataa aaatgatccg atacagcaac caatgataaa ataaagtaac acgccatctc
     6661 ccccttttca aaaccaagat acgcaagctt aaactaaatt tttgtacata cataaaattt
     6721 gaggtgaaaa aatgaacgtt tcaatcggat tagatatcgg aaccacacaa accaaagcag
     6781 ttgctttcac agatgaagcc caagaagcag catccgctta ttttcgctac cctttgatcc
     6841 aagaaacaca agggatggca gaacaagatc aggaattgat ctttcaaggt gtctgcaccg
     6901 ttataaaaga agtaattaaa cagttgcctt cagacacaat cattcaattt gtctccttct
     6961 cctctgcgat gcatggcctg atattgttgg atgaagagaa aacaccgtta tctcgaatga
     7021 tcacttgggc agataaccga gcagcggacg aagcagaaaa actgaaatat accgtgcttg
     7081 gcaaagaatt ttatcggcag actgggacgc cgattcatcc aatgacacca ttgaacaaaa
     7141 tcaagtggct gcaaaacagc caacctgaat tattcaaaaa aactgcttat tttgtcggta
     7201 tcaaagacta tatcttttat agattgtttg gcgaactgat cagcgactac ggaacagctt
     7261 caggaactgg atattttgat atccatgaat tccaatgggc agaagaagtg cttgactact
     7321 tgaacattcg aatagagcaa ttgccgcaag tctttccttc cacctatcaa gtaacaggtc
     7381 taccatcaaa aacagcagca gaactaggtt tgccaaatga tatatcattt gttttgggag
     7441 gggcagacgg gccgctttct aatctagggt taggtgcttt tggtgatacg atcgccgctt
     7501 tgaccgtagg aacgagcgga gcacttcgtt ttattactac tcagcctcag ttccatccag
     7561 aaatggagac gttttgttat gttctggatc agaaacattg ggtagtggga ggagcgacta
     7621 gcaatggtgc tggcatcttt gattgggcag ggcatacatt tttacaggaa gtcgttcaat
     7681 ctgctaaaga aaaaggagaa aatccatatg atgcactgct ttcggctatc gcttttgtgc
     7741 cagcgggtgc caatggtctg atttttcatc cctatttact tggtgagcga gcaccgcttt
     7801 gggacgcaga tgcgtttggc agttttatcg gtttacagcg gcaacataca gaaaaagaaa
     7861 tgatgaaggc gattctagaa gggatctgtc tcaaccttta tcggatttta aaaggagttt
     7921 gccaggaaaa tcagctcaca gagatccgtg ctacaggcgg gtttgctcaa tctccgattt
     7981 ttcgtcagat gatggctgat gtactgggtt acccgcttgt tttccctact gtcacagaag
     8041 catccgcatt aggagctgtg attttaggct ggagaagcat cggaaagatc caaagtatca
     8101 gtgaagcaca ggaattgata gaattgaaag agcaagttgg agtacaagaa actgatcata
     8161 aaaaatatca gaaaatctat ccattatttg tttcgacaca aaagcattta gcacagactt
     8221 atcagcaatt tgctcaactg cgagaagaat tagctgattc tgaaaaatga ttggctttac
     8281 ggataaaaaa gcgaactgaa aatcgttctc aggtaaatga ttttgttgta cttttcattg
     8341 aaagtctact attattttaa gtgtaagaaa atgttaggag gcaagaaaga tgaaattcca
     8401 acaaaccaaa gaagtattaa atcaattagt tgcagatcta agccaaatgt ctgttgttgt
     8461 tcaccaaaca cattggtaca tgagaggacc aggtttcctt acattgcacc caatgatgga
     8521 tgagttcatg gatgatttga acgctcagtt agatgaagta tccgaacgct tgatcacatt
     8581 agacggttca ccttattcta cattgcgtga atttgcagat aacacaaaaa tccctgatga
     8641 aattggccgc tgggatcgta ctattccaga acgtttggaa acacttgttg caggctatcg
     8701 ttatctagca gatctttacc aaaaaggaat cgaagtttct ggagaagaag gcgacgatcc
     8761 aacacaagat atctttatcg aattcaaaac agccacagaa aaacgtatct ggatgatcca
     8821 agctcatttg ggcaaagcgc cagagatcga cgcgtaaaaa ctgttgttga acttgaccga
     8881 aaaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac gtcaaaatag
     8941 ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
     9001 accaagaggt tgtaagcctg ccgttcagct ccgagtcaca agaaacaatt ttaaaaaaca
     9061 gcttcttatg ttttatggat attgtgactt gtgaccgagg agttggcagg agaacaccgt
     9121 ttattcaaaa taaggggttg tgacaagact tttgtcacag cctctttttg tttttcattt
     9181 tgacaggaaa cgaccctttt atataaaaga aagaaaagaa gtaaagtttc atataaattt
     9241 ttcaatcaag cagaaaaacg ttgacattga ctagattagc gggtaatatg ttaaatggta
     9301 ttgtttgccc cacaaagagc cccggaaact cgaagtgtat gtcaaacatc agaacttaaa
     9361 ttggaggaaa ataacgtgcg tacaacatat atggccaaac caggcgaagt agaacgtaaa
     9421 tggtatgtag tagacgcaac tgatgttcca ttgggacgtc tttcaacagt tgttgcatct
     9481 gtactacgtg gtaaaaataa accaactttc acacctcatt tggatactgg cgatttcgtc
     9541 atcgtcatca atgcagacaa agtgaaatta acaggtaaaa aagcgactga caaaatttat
     9601 taccgtcaca gcaactaccc aggaggattg aaatcaatca ctgctggcga attgcgtgct
     9661 aaaaattctc gtcgtttgat cgaaacttct gtaaaaggta tgcttcctaa aaacacttta
     9721 ggccgcaaac aatttacaaa attgaacgta tacggtggag cagaacatcc acatgcagca
     9781 caacaaccag aagttttaga catcacaaac ttaatctaat aggagggaat ttcattggca
     9841 caagttcaat atatcggcac aggccgtcgt aaaaacgcag ttgctcgcgt acgtctagta
     9901 ccaggaactg gcaaaattac tgtaaataaa aaagatgttg aagaatacat tccacatgct
     9961 gacttgcgtg aagtaatcaa tcaacctttc gctgttactg aaacaaaagg cgcatatgac
    10021 gtatttgtta acgtaaacgg cggaggatac gctggacaat caggagctat ccgtcatggt
    10081 atcgctcgtg cattgctaga agttgatcct gacttccgtt tagctcttaa acgtgctggc
    10141 ttacttactc gtgacgcacg tatggtcgaa cgtaaaaaac caggtcttaa gaaagcacgt
    10201 aaagcttcac aattctcaaa acgttaatat cctttatata tcaacgtttc tggcactttt
    10261 caattatttg gaaagtgctt cttttgtttt taagaacaca tttaagaaca catatcaatc
    10321 acacttattc aatcctctct gaaaagcttc aaaaacagcc ttatcaacta tatctggtgt
    10381 gttcacatat atctttgttg tagaaatatc tgagtgtgtt aaagcatttg aaatttgatt
    10441 catatctgct ccaccttgtc tagctatagt tgcataagta tgacggagtt tgtgtggaga
    10501 taaatgaact aagtcaggat gccttctttt aattgaattg attcgatagt ttaggtaatc
    10561 ggcatgaaca ggacaattta cttctccaga tggtttagta taagtaaaga gatactgatc
    10621 tgacgtttgc ttaattttta atttcaacaa ttgatcagct tgtaccgatt tccattcact
    10681 tagcaatgtc attacaactt ctggtacttg aatgacggta tcttttcgac cttttgtaaa
    10741 cttttttctt tgatatttat ctaaagtatg tttaagacgg accgtttgtt tttcaaaatc
    10801 aatatatttc cattgcaaag cgtaagtctc actttttcta tcaccaagat acagggtaat
    10861 cataaaaagt gtgtaatcat gaaaagtaag taaatgatta ttaaaatcat catttactgc
    10921 ttctatccaa ttagtaagtt cttttgatga tagtgcttgc ttaccttctt gtattctttt
    10981 ttcttctaaa gcattttttt taggtgcagt gatactttga ataattttag tagttcgatt
    11041 aaattcgata taatttagta tttctgcaat atcgaacatg gaacgaacat atccttttat
    11101 gatttttata tttgcatatt ctttcgattt tttggttaat tcatctgata ttatctcagt
    11161 attgatattt agataattca ttgcgtattc accaaacata ggtaaaatat gcaaccgaaa
    11221 tatatctttt gtattactaa tggttatatc actaggaggc ctgtttgatc taattgtttg
    11281 acctaattca tatctaggaa gccatttaga ttcgtaaaat tttttaaacg ttatttttcc
    11341 ttttaattct aatgagtttg cattctcctc tctcaaaact ttttctattt tcttcttcat
    11401 atcattttcg gctttaatcg ccaattgcct atttggaaat gttttttgat acttttctga
    11461 tgcaattcct gttatttctt taaacctttt tggatatctc agattagttt tatatttccc
    11521 atttgaaagc tttgtaggca aaatcctcac tccttaaaaa aagtaaggca gtatatttca
    11581 ctaattttag tatatgcggc gagaagaaaa tttcaagtta ttttaaatgt attcataaag
    11641 tctttaatat cttctacgtc aaagcgaact acaccaccaa ctcttattac tgggaaatta
    11701 tatatacgaa tccacttatc tagagtatta ttatcgattc ctagaaatgc acaggtttgt
    11761 tttttattca ggtagactct ggattctata aaatcatctc ctaaaatagt agttatgaaa
    11821 tttttgaatt gctttgggta agcacatagg aatttcacct ctccccagtt tacggcgagc
    11881 cttacggaag tatcattatt tgctgctatt gtcatggcat tttgttttcc cagaacaaaa
    11941 agaaatctct aagtatttgt cgctaatcag gtcatggcgt acttgagttt ctagctcgaa
    12001 tagcagggat cgttattcaa aaattgtatt taattgtcaa tgatcaaaaa tagttttttt
    12061 cctattcact attaataacg ataaacatgt ataaaaagac acactacgaa aaaagttaaa
    12121 tggagtttac tgattttcat atttgta
//
LOCUS       DAXWLH010000074        12136 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXWLH010000074 DAXWLH010000000
VERSION     DAXWLH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.074"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..610
                     /locus_tag="ACGE36_001604"
     CDS             <1..610
                     /locus_tag="ACGE36_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8383994.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACGE36_001605"
     CDS             723..1001
                     /locus_tag="ACGE36_001605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8383995.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACGE36_001606"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACGE36_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFF8383996.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACGE36_001607"
     CDS             1458..2213
                     /locus_tag="ACGE36_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8383997.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE36_001608"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE36_001608"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFF8383998.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE36_001609"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE36_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFF8383999.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE36_001610"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE36_001610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFF8384000.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE36_001611"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE36_001611"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFF8384001.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE36_001612"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE36_001612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFF8384002.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACGE36_001613"
     CDS             6950..7492
                     /locus_tag="ACGE36_001613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFF8384003.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE36_001614"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE36_001614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFF8384004.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACGE36_001615"
     CDS             9085..9987
                     /locus_tag="ACGE36_001615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFF8384005.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE36_001616"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE36_001616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFF8384006.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACGE36_001617"
     CDS             11430..11852
                     /locus_tag="ACGE36_001617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFF8384007.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAXWLH010000075        11971 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXWLH010000075 DAXWLH010000000
VERSION     DAXWLH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.075"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(148..312)
                     /locus_tag="ACGE36_001618"
     CDS             complement(148..312)
                     /locus_tag="ACGE36_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384008.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            607..1935
                     /locus_tag="ACGE36_001619"
     CDS             607..1935
                     /locus_tag="ACGE36_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFF8384009.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2137..2970
                     /locus_tag="ACGE36_001620"
     CDS             2137..2970
                     /locus_tag="ACGE36_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFF8384010.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2986..3723
                     /locus_tag="ACGE36_001621"
     CDS             2986..3723
                     /locus_tag="ACGE36_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFF8384011.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3878..4846)
                     /locus_tag="ACGE36_001622"
     CDS             complement(3878..4846)
                     /locus_tag="ACGE36_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFF8384012.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5078..5911
                     /locus_tag="ACGE36_001623"
     CDS             5078..5911
                     /locus_tag="ACGE36_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFF8384013.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5945..6784
                     /locus_tag="ACGE36_001624"
     CDS             5945..6784
                     /locus_tag="ACGE36_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8384014.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6810..7370
                     /locus_tag="ACGE36_001625"
     CDS             6810..7370
                     /locus_tag="ACGE36_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8384015.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7569..9290
                     /locus_tag="ACGE36_001626"
     CDS             7569..9290
                     /locus_tag="ACGE36_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFF8384016.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9455..10597
                     /locus_tag="ACGE36_001627"
     CDS             9455..10597
                     /locus_tag="ACGE36_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8384017.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10613..11824
                     /gene="thiI"
                     /locus_tag="ACGE36_001628"
     CDS             10613..11824
                     /gene="thiI"
                     /locus_tag="ACGE36_001628"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFF8384018.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4073 a         2020 c         2488 g         3390 t
ORIGIN      
        1 actgccggag taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt
       61 ttcttttatt attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta
      121 cgaaagagta gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa
      181 ataaatcagc attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt
      241 cccaaagtta tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat
      301 caggtttttc attttattca tctcctttct tacgttatag tatttccttc tttcaatgag
      361 tattttgaaa gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc
      421 cattttcgtt atctcataga aatattcctt tttatctgaa aactattgta taccgtatga
      481 catttttctt catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag
      541 agattgtatt tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat
      601 gattggatga aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca
      661 aatatcaaac agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct
      721 gaaaatgttt cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg
      781 aaagtggcta atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc
      841 cgtcataaga tgctgaatca attccattta aaagagtttg aattatcaca aaattacact
      901 tttttctggg ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga
      961 aaagaggcaa tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc
     1021 ggccaatggg atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg
     1081 atgccggaaa gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta
     1141 ctgcgtaaag atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct
     1201 attgaagaaa agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta
     1261 ggaacacctc caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt
     1321 gaccgaggac tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat
     1381 tatgttagcg tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta
     1441 gagatgctag gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa
     1501 actttcaaaa aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt
     1561 gatgtaggtc aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg
     1621 gatgatctat ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa
     1681 agtctgatga cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca
     1741 aaatggaaag tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg
     1801 agtgatgcct ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg
     1861 aaagagcaat taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg
     1921 ggtgctttag catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg
     1981 gcttaagcca gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct
     2041 ataattctaa ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga
     2101 cgaagtagaa taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct
     2161 gatctgaaat taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat
     2221 cgtataaaaa ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga
     2281 ttttttgaag cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat
     2341 acgtatatgc taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg
     2401 ggaaaagggg atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg
     2461 ccacaatttg gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt
     2521 gaagaagatt ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca
     2581 gaagaaacga atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt
     2641 gtattgaaat cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg
     2701 agtggtaatg tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct
     2761 tatgggaatc gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa
     2821 tttttagatg cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg
     2881 atttaccagt tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc
     2941 caagtagatc aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat
     3001 accaaaaagc atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct
     3061 tcgatgttag gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc
     3121 agtgaattcg tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt
     3181 aatcgtctcc ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat
     3241 aagtttttct gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa
     3301 atagaatctg ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa
     3361 gagatcattg ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt
     3421 gaaaagattg attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt
     3481 aatcagcatg ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac
     3541 tttttgacta cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac
     3601 gggaatgaga tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa
     3661 catgaaatcc gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa
     3721 taaaaaagag gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa
     3781 ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca
     3841 gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca
     3901 attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac
     3961 tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat
     4021 aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact acagggattt
     4081 tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta
     4141 aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt
     4201 gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc
     4261 gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg
     4321 gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc
     4381 gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac
     4441 aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac
     4501 ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa
     4561 aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg
     4621 atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg
     4681 gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt
     4741 ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt
     4801 tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct
     4861 tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta
     4921 tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga
     4981 aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga
     5041 aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc
     5101 gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg
     5161 gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt
     5221 tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg
     5281 aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca
     5341 ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca
     5401 attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa
     5461 gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga
     5521 atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga
     5581 tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt
     5641 tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg
     5701 gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga
     5761 agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg
     5821 tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg
     5881 actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa
     5941 gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa
     6001 agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg
     6061 tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga
     6121 tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca
     6181 cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca
     6241 aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc
     6301 gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa
     6361 aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat
     6421 attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt
     6481 agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat
     6541 cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga
     6601 aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt
     6661 aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt
     6721 gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta
     6781 ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag
     6841 aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa
     6901 atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta
     6961 cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata
     7021 aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag
     7081 tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag
     7141 tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg
     7201 gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg
     7261 aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg
     7321 ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc
     7381 actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat
     7441 gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt
     7501 tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg
     7561 aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca
     7621 ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct
     7681 tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta
     7741 aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga
     7801 atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc
     7861 tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa
     7921 tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa
     7981 cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag
     8041 aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga
     8101 tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg
     8161 ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga
     8221 tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag
     8281 aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc
     8341 ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa
     8401 aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt
     8461 tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa
     8521 cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg
     8581 atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc
     8641 aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac
     8701 acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag
     8761 atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa
     8821 aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg
     8881 tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta
     8941 ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg
     9001 tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg
     9061 atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc
     9121 atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat
     9181 atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg
     9241 ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag
     9301 ttagtaaacc agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt
     9361 tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat
     9421 aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac
     9481 aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg
     9541 gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag
     9601 aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg
     9661 aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa
     9721 tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa
     9781 agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga
     9841 cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa
     9901 tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa
     9961 aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt
    10021 gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac
    10081 tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca
    10141 agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc
    10201 tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt
    10261 agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga
    10321 ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca
    10381 tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca
    10441 tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt
    10501 tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt
    10561 caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata
    10621 cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt
    10681 tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat
    10741 ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat
    10801 tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa
    10861 agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa
    10921 agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga
    10981 attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat
    11041 gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta
    11101 tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat
    11161 gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga
    11221 aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc
    11281 aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc
    11341 atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat
    11401 tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt
    11461 cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc
    11521 tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga
    11581 aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga
    11641 ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca
    11701 tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa
    11761 gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct
    11821 ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata
    11881 gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt
    11941 atcaagaagt tatggaaaag ccggttcttt t
//
LOCUS       DAXWLH010000076        11945 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXWLH010000076 DAXWLH010000000
VERSION     DAXWLH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.076"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(920..1432)
                     /locus_tag="ACGE36_001629"
     CDS             complement(920..1432)
                     /locus_tag="ACGE36_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFF8384019.1"
                     /translation="MKNYYFFFLSCFCLFGLTSCGWIFSEEKTEVSLISKEKMIQAES
                     SSKESKRNQEDKTECNISEEIQYLLKNFGRKWVNYTDIYERNQSVKNYLTDRCIKENG
                     IDNDPHVQYKTIGRIHTITQDVIQSDQYLLFGEETTNEMTRFVLLQIKVDQEVQKIDK
                     IKIYYVRSAY"
     gene            complement(1483..2541)
                     /locus_tag="ACGE36_001630"
     CDS             complement(1483..2541)
                     /locus_tag="ACGE36_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8384020.1"
                     /translation="MMSCLFLLFAIISMSSGESKHMDSAVGVTHTGEYIETLPIFSEI
                     KGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRNDTNFFGITWFPG
                     CPYPQGSARGIGGSEGGFYMKFPNLNACYDYYGFMVASQSNFNQCVGNKDPGSCLLIL
                     GRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDAFAIKHWNSLPTKKTSLIAGKSKGN
                     AKVLDRIIGRPVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGEDYNWATYGWQVI
                     MNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSNSGQSFSTVEQNAEQGRIMAR
                     YQRSYNQAKIRSLVRKVK"
     gene            complement(2574..3362)
                     /locus_tag="ACGE36_001631"
     CDS             complement(2574..3362)
                     /locus_tag="ACGE36_001631"
                     /inference="COORDINATES: protein motif:HMM:NF024056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8384021.1"
                     /translation="MVFSVCFGVYNNLTSQDTHTIHETKVIDKQLKDMTGIESYVKNF
                     AFLYFTVSLESDQQINRSKAVESYLADGLQMDNEIDKDIETNVKVKNVSIWAIEEHES
                     KKHTFVVIFHVLLEIEKEFKERVYKVEVYRNELSYAIVKLPIVASNVKKATIKVDTRF
                     ETTSISRKEQEELEKFLYTFFRIYPKANAEELKYYGTTLLPIQQPLAFEELSNLQVAK
                     KEGRYEIHCNILYQDQETHLWFTMYYEMSMKQLENGNFRIEAIH"
     gene            complement(3509..5530)
                     /locus_tag="ACGE36_001632"
     CDS             complement(3509..5530)
                     /locus_tag="ACGE36_001632"
                     /inference="COORDINATES: protein motif:HMM:NF046089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8384022.1"
                     /translation="MKQWYSKVSFSITRRKLVFISLVIMMGVFFFHPIFVEAAGWIKE
                     TLGGKNAVYDFETYPLDNYNLDFFVDTSWNWLPWKWGEGISNTVIYGIFAVTNILWML
                     NVYLCYFLGFLVQEAFELDFLSELIGSLSHSIQNIAGMNRAGTLSHGLVPFFGGLLIA
                     LAGCSVVYQGIVKQHPTQAIQQAVTFLFTFICCGIFFMNTHTYLEKANEVQKEINSEM
                     LTIAKDLLPEQKGKPDAIKAIRENLFAIQIKQPWLVLQFGDSDIKTVGEERVINLLKE
                     SPYDEGEQRNKLVEEEVTKQKNGNLSVQKVYLRFGMVFLTLIVNIVISMGVLALVVIL
                     IASQVLFLLFIGFLPVAMIFSLFPNSGSLLGNALQKVFQLLFTKMGILLILTITFSIS
                     HELNHLSKNKGMIWTSFLQVTLWFSVSTRVNELLGWMKIGGAQTRAGDRTGRFLRGMM
                     LGTMGQKMIRGIGENFVGGAAGGVADQMISKSMSGQRSITTTPLIERVGKKVSAMTEL
                     PATIESRAEQWKRTIQYVPTNAQYKAQKMKYDYFSGRMNESERQTRARNLANERQKNF
                     YEARKDALQLEQQERKQQAMRSMAHQTGKTQVTSGKLQTRKSAMIGRTEQFPTIEKRS
                     NISNVAKNKKLSKQEHPIHERSTNRKIETPVRWTSTSCQNARKGRTKKE"
     gene            complement(5531..8068)
                     /locus_tag="ACGE36_001633"
     CDS             complement(5531..8068)
                     /locus_tag="ACGE36_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8384023.1"
                     /translation="MRLPKLVPKIPFYYLEDNLVFGEEDRVYAYYEFPMYSYSFVGED
                     QAYQLQQAIMRLIRQSYSSHVRFFFCTNEEKIEKTIKASKELVRSEGELKTIAYQHLE
                     GVQHILEELHGKYIQNVCWYVGIELVLEESAPKEAHWMKEFRIATKDFFHRSNARLFG
                     DFETIPNEKIQRYKRLENLLYNRISKYFRLRKTTPEDMVFLVEHLEGKKEQSLDEVMY
                     RPNSFKEETNTYTYHYDVLNLASAGITEKKNYLILEQGTEKEYITYLSLSRMTRDLVF
                     PFQSEIYYFLQSELDFPIDSWLDVNLLDNRLALAKVRGKKSDLSDIYESGMSVGKDVG
                     NNVREAYQSANELEQKLEDTREDLYVLSLNVRVSGKTYDEMLKRVSDVRNFFDVYSMY
                     LEVPCNVQLFLHFGSYPAGRRVIEDYRQYVGAEFIASLGIGATQQLGDGCGIPIGFNA
                     ETGIPVFIQPWLAAQGIKGTVTNTLAKALIGSLGGGKSLTENLLLFWSVLFGALAFCI
                     DPKSERSNWSEDLPYFAPYLKTVNITSEKENIGLLDPFQLMRRKKDQESLALDVLTYI
                     TGVSIRDEDRFPLLQRAVKTVSDFEGTKGLLLVIQELYAQEAAIAQRLAKHIESFKEL
                     SIAGLLFGDGTNQQGLNLDAKCNLALVQDLTLPDSETAPEDYNSSEILSIAIMMSLAT
                     YALDFIKQNRDIYKAVGLDESWAWLNVAQGKILGNKLVREGRSRNAGIDFSTQNTDDL
                     GDEKMRSKIGLKFIFRSNDREEITKALNFCGISVTEENIQRVKNLENGECLFCDIHGN
                     IGILYVYYWFEDLFHAFDTRPPMEKNYEEEEYKDEILEKLVEEIEVN"
     gene            complement(8090..8449)
                     /locus_tag="ACGE36_001634"
     CDS             complement(8090..8449)
                     /locus_tag="ACGE36_001634"
                     /inference="COORDINATES: protein motif:HMM:NF024062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFF8384024.1"
                     /translation="MNKIRNWTKIWYIERVFYKLWDGVALPRPVTQTFAIWFGIGFFG
                     GIFRIFPFVMEHTITRQFFCPWLFAFLMTRKQFQGRKPYNYLASFLLFQCRSKESFHG
                     QWVKPIEKKKSNEEFVV"
     gene            complement(8451..8669)
                     /locus_tag="ACGE36_001635"
     CDS             complement(8451..8669)
                     /locus_tag="ACGE36_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384025.1"
                     /translation="MNFFQNVFTDFVGDFKYLIMLAMLGGAAYIAFERKVSKAIPLIL
                     VLAVAVWLVGDTSGVFNWILDRMRSWGR"
     gene            complement(9191..10171)
                     /locus_tag="ACGE36_001636"
     CDS             complement(9191..10171)
                     /locus_tag="ACGE36_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8384026.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFDRASRFAEEMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVNKAKENNLGKLIY"
     gene            complement(10184..11059)
                     /locus_tag="ACGE36_001637"
     CDS             complement(10184..11059)
                     /locus_tag="ACGE36_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8384027.1"
                     /translation="MRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPFT
                     SFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAINV
                     GIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKVFVGIGLVIAALLIFAGQMNWIPSNG
                     TASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACTF
                     SVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYVLKWIVILVLLYSA
                     YTMLGSQLKKVSAAN"
     gene            complement(11078..11878)
                     /locus_tag="ACGE36_001638"
     CDS             complement(11078..11878)
                     /locus_tag="ACGE36_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFF8384028.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENLTDSLLKTLQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESSEELKAKYGEDFEGIRKIY"
BASE COUNT         3577 a         2524 c         1789 g         4055 t
ORIGIN      
        1 atttataaaa ttaaatggtt tatgaaacaa tgttaacaag aaggagctac taagtcaaat
       61 aaaatgaacg ttcaatttgt ttttttaact cgagcatttt ttcatgaaaa atcaaacaat
      121 caattataaa agacacagca ctatctttct tattcaaaac tagtaaacat tgtagcttct
      181 atatttgtca agaaaggatc ataatttgtt cggaaatgaa aaatactcta tttagaattt
      241 ttttcgataa aaaataaaag tataaattat ttttctgaaa gttttaatgg taatgaacgt
      301 taattttata aggtaaaagc tatgagtgta agagcaaatt tgttgagata acgtgtaggt
      361 attcttataa tagacttggg aatattccta tatcactctc taggggtagc tcaggggaag
      421 gagttgcccc tttaaatgta tattagaata atcaagataa ccaggtgctt tgaaattctg
      481 aatctattta tccaattcaa ctgtgtttcc tatagagttt atacaggaac ccatcgatca
      541 taatcaattg ttctgaaatt acatctaact gattataaat ttgctctaca agctcatccg
      601 cttttgtatg tagcgataga aatttgcgtc cgtgtatcta cttaggatca ttttttcaca
      661 aattcatccc atgattcact aaatcatggt tcaaaaagac atttttcaac tctagtactt
      721 gttttaacca aagtacttct actttttccg tttctatctc attcctcctc ctattttttg
      781 tacattattt tcttatctct ttccttttaa ttgatttatt cccaaaattc ttcatattct
      841 ttaaacatct taaatagttc agcttcttcc ttttcgttaa actataattt ctagtaaata
      901 aaacaacgtc atttttcttt taatatgcac ttcttacata ataaatcttg attttatcaa
      961 tcttctgcac ttcttgatcc accttgattt gaagtaacac aaaccgagtc atctcatttg
     1021 ttgtttcttc tccaaataat aaatattgat ccgattggat tacatcttgt gtgatagtat
     1081 gaatacggcc aattgtttta tattgtacat gtggatcgtt gtcaatccca ttttctttta
     1141 tacaacgatc ggttaaatag ttttttacag attgatttcg ttcgtatata tccgtgtaat
     1201 tcacccattt tcttccaaaa ttctttaaca aatattggat ttcttcagaa atattgcatt
     1261 ctgttttgtc ttcctgattt cttttgcttt ctttacttga actttctgct tgtatcatct
     1321 tttcttttga tattaaggaa acttctgttt tttcttcaga aaaaatccac ccacaactag
     1381 taagtccaaa caaacaaaaa caacttagaa agaaaaaata ataatttttc acaatcgatc
     1441 cttctccctt ttatactaaa aattttcatc gaaagaattc ttttatttca ctttccgaac
     1501 cagacttcga attttcgctt ggttatagct gcgttgatac cgtgccataa ttcgcccctg
     1561 ctcggcgttc tgttctacgg tggagaaact ttgtccacta tttgagacat ctgttatgat
     1621 acccgtgtgg ccatagattc caggagacaa tgcaccacct gcttgccaat tgaccacatc
     1681 tcctgcttgt aagtcactgg gctttggatt catgatcact tgccaaccat aggttgccca
     1741 gttataatct tcccctatta acatcgcaaa catgtgtcca gaacccatta actgtgggcc
     1801 acctagttga gatacatacc atgccgtacc accataacat tccccattat ttacgggtcg
     1861 tccaatgatc cgatccaaaa ccttcgcatt gcctttactt tttcctgcaa taagcgaagt
     1921 ttttttggtt ggtaggctat tccaatgctt gatcgcaaag gcatcatatt ccgttaagtt
     1981 ataggacttt atgatactca ttgcgccttg ataatacgga ctgccttcgg tgatacctgc
     2041 cgccgcataa cctccacgac ctagaatcaa taaacaactt ccaggatctt tattgcccac
     2101 acattgatta aagttgcttt gactcgccac cataaatcca taatagtcat aacaagcatt
     2161 caggttcgga aacttcatat agaaaccacc ttcagagcca ccaatcccac gtgcagaacc
     2221 ttgtggatag ggacaaccag gaaaccacgt aatcccaaaa aagttcgtat cgtttcttcc
     2281 agattgtgac tgcccataag ctgattcata tcctaattga gaaagaatca cactcggtaa
     2341 taactgatac tttacagctg caccaactgc tgcttgggca tgttcatctg ttatcggacc
     2401 acgccctttg atctctgaaa aaataggtaa ggtttcgata tattctcccg tatgagtaac
     2461 accaactgct gaatccatgt gcttcgattc accactagac atgctaatta tcgcaaataa
     2521 taaaaacagg caactcatca tacagatgcc actaaataaa aaaatctttt tcactagtga
     2581 atcgcctcaa ttcgaaagtt cccattctct aattgtttca tagacatttc atagtacatg
     2641 gtaaaccaaa ggtgcgtttc ttgatcttga tacaaaatat tacaatgaat ctcatatcta
     2701 ccctcttttt ttgcgacttg caaattcgat agttcttcaa aggctaaagg ctgttgaatt
     2761 ggtaaaagag ttgtcccata atattttaat tcttctgcgt ttgctttagg ataaatacga
     2821 aagaaggtat ataagaattt ctctaattct tcctgttctt ttcgagaaat gctcgttgtt
     2881 tcgaaccgag tgtctacttt aatcgttgct tttttaacat tactcgctac aataggcaat
     2941 ttcactatgg catatgataa ttcgtttcga tatacctcca ccttatacac acgctctttg
     3001 aattcttttt ctatctctaa taagacatga aatataacca caaatgtatg tttttttgat
     3061 tcatgttcct ctattgccca aatcgagaca tttttaactt ttacattcgt ttctatgtct
     3121 ttatctattt cattatccat ttgcagccca tcagctaaat aactttctac tgcttttgat
     3181 cgatttattt gttggtcgct ttcaagactc actgtaaaat acaaaaatgc aaaatttttc
     3241 acataacttt ctattccagt catatctttt aattgtttat caatgacttt tgtttcatgg
     3301 attgtatgcg tatcttgaga agttaaatta ttataaactc caaaacaaac actaaaaacc
     3361 aaaaagctcc agcctacaaa aaaaaggaag cgcctttttc gcgctccctt atagctttct
     3421 tttttcttgt ctttctttcc ttttagtctt gtttcttgtt cagagagaat agacagtttt
     3481 ttcttccgag gaaacatagc cctcctcctt attctttttt agttctacct tttctcgcgt
     3541 tttgacaaga agtcgaagtc caacgaaccg gagtttcaat ctttcgattc gtacttcgtt
     3601 catgaatcgg atgttcctgc ttcgacaatt tcttattttt agctacattt gaaatgttac
     3661 ttcttttttc tatcgtagga aattgttctg ttcgaccaat cattgcactt ttccgtgttt
     3721 gtaatttacc agaagtcact tgtgtttttc ctgtttgatg cgccattgaa cgcatcgctt
     3781 gttgttttct ctcttgttgc tctaattgca atgcatcttt tcgtgcttca taaaaattct
     3841 tctgtcgctc attcgcaagg ttacgcgctc gtgtttgtcg ttccgactca ttcattcgcc
     3901 cagagaaata atcgtatttc attttttgtg ctttgtattg ggcattggtt gggacatact
     3961 gaattgtccg tttccattgt tctgccctac tttcaatcgt tgccggtaat tctgtcatag
     4021 ctgaaacttt ttttcctact cgctctataa gaggtgtagt agttatactt cgttgaccac
     4081 tcatagattt ggaaatcatt tggtcagcta cacctccggc agcaccacca acgaaatttt
     4141 caccaattcc tcgtatcatt ttttgaccca ttgtacctaa catcatgcca cgtaaaaaac
     4201 gtcccgttcg atccccagct cgtgtttgtg cgccaccaat tttcatccaa cctaataatt
     4261 cattgacacg tgtcgagact gaaaaccata acgtcacctg taagaaactg gtccaaatca
     4321 ttcctttgtt cttggataag tggtttaact cgtgactaat agaaaaagta atggttaaaa
     4381 tgagtaaaat gcccattttt gtaaacaata attgaaagac tttctgtaag gcattaccta
     4441 ataacgatcc tgaatttgga aacaacgaga aaatcatcgc tacaggtaaa aatcctataa
     4501 ataacaagaa caatacttgc gacgcaatta agatgacgac taatgccaat acacccatac
     4561 taatgacaat gttgacaatg agcgttaaaa acaccatacc aaaacgcaaa tacacttttt
     4621 gtaccgaaag attgccattt ttttgtttgg ttacttcttc ttcaacaagc ttatttcgct
     4681 gctctccttc atcataagga gattctttta ataaattgat tacacgttct tcaccaactg
     4741 tttttatatc gctatcccca aattgcaata ctaaccaggg ttgcttgatt tgaatggcaa
     4801 atagattttc acgaatggct ttaattgcat ctggttttcc tttttgttca ggtaacagat
     4861 ctttggcaat ggttaacatt tcactattga tttctttttg tacctcattg gctttctcta
     4921 aatatgtgtg cgtattcata aagaaaatgc cgcaacagat aaacgtaaat aaaaatgtca
     4981 ctgcttgttg aatcgcttgc gtagggtgtt gtttcactat cccttgataa actactgaac
     5041 aacctgctaa agcaattaac aatccaccaa agaaagggac taatccatga ctcaatgtcc
     5101 ctgcacggtt cattccagca atattctgaa ttgaatgact taacgaacca atcaactctg
     5161 aaagaaaatc taattcaaag gcttcttgga caagaaaccc taaaaagtaa cacagataga
     5221 catttaacat ccataaaata ttggtaacgg caaatatccc ataaataact gtattagaaa
     5281 ttccttcacc ccatttccac ggcaaccaat tccacgacgt atccacaaaa aaatctaaat
     5341 tataattatc taaaggatat gtttcaaaat catatacggc atttttgccg cctagggttt
     5401 cttttatcca accagctgct tctacgaata ttgggtgaaa gaaaaaaaca cccatcatta
     5461 tgactagact tatgaaaacc agttttcggc gagtaattga aaatgaaacc tttgaatacc
     5521 actgtttcat ctaattcacc tcaatttctt caaccagttt ttctaatatc tcgtctttat
     5581 attcttcctc ttcgtagttt ttttccattg gcggtcgtgt atcaaatgca tggaataaat
     5641 cttcaaacca ataatacaca tacaaaatgc caatattacc atggatgtca caaaacagac
     5701 attctccatt ttctagattt tttacacgct ggatattttc ttctgtgaca gagattccac
     5761 aaaagtttaa tgctttggta atttcttcgc gatcattcga acgaaaaatg aatttcaacc
     5821 caattttact tctcatcttt tcatcgccta aatcatctgt gttttgtgtt gaaaaatcaa
     5881 tccctgcatt gcgagatctt ccttcacgaa ccaatttatt tcctaaaatt tttccttgcg
     5941 caacattcaa ccacgcccaa ctttcatcta agccaaccgc tttataaata tctcgatttt
     6001 gtttaataaa atctagcgca tatgtagcta aactcatcat aattgctata cttaaaattt
     6061 cagaagagtt atagtcctct ggtgcagttt cactatctgg taacgttaaa tcctgaacca
     6121 gtgcgagatt acatttggca tccaaattta acccttgttg attcgtgcca tctccaaata
     6181 ataggcctgc aatagatagt tctttgaagc tttcaatatg ctttgccagg cgttgtgcga
     6241 tcgctgcttc ttgtgcatat aattcctgaa tcactagaag taatcctttt gttccctcaa
     6301 aatcagaaac agtttttact gcccgttgta acaaaggaaa ccggtcttca tcccgaatac
     6361 ttacacccgt gatataggtg agcacatcca aggctaaact ttcttgatct ttttttcttc
     6421 tcatcaattg aaatggatca agtaaaccaa tattttcttt ctcactggta atattaactg
     6481 tttttaagta gggggcaaaa tatggcaaat cttcggacca atttgaacgt tccgattttg
     6541 gatcgataca aaaagccaag gcgccaaaca acaccgacca aaataacaag agattttcag
     6601 ttaaggattt cccaccacct agcgaaccaa ttaatgcttt ggcaagagta ttggtaaccg
     6661 ttccttttat tccttgtgcc gctaaccatg gttgaataaa cactgggatt cctgtttctg
     6721 cattaaatcc aatcggaata ccacagccat ctcctagttg ttgtgtcgca ccaatgccaa
     6781 gactagcaat aaattctgcg cctacatatt gacgataatc ctcaatcact cgccttcctg
     6841 ctggataact tccaaaatgt aaaaacagct gtacattgca cggtacttct aaatacatag
     6901 aatagacatc aaaaaaattg cgaacatcac tcacacgttt taacatttca tcataggtct
     6961 tccctgacac gcgaacgttt agtgataata catacaaatc ttctcgcgta tcttctaatt
     7021 tttgttccaa ctcattggct gattggtacg cttcgcgtac attgtttcct acatcttttc
     7081 caaccgacat accactttca taaatatcag ataaatcact ctttttgcca cgtactttcg
     7141 ccaaggccaa acgattgtct aaaagattca catctaacca agaatcgatt ggaaaatcca
     7201 attccgattg taaaaaataa taaatctctg attgaaatgg aaataccaaa tcacgggtca
     7261 tacgtgaaag agataaatac gtgatatatt ctttttctgt cccttgttct aaaattagat
     7321 aatttttctt ttctgtgatt cctgcactgg ccaaattcaa tacatcatag tgatacgtat
     7381 acgtattcgt ttcttcttta aagctattgg gtcgatacat cacttcatct aaggattgtt
     7441 ctttttttcc ttctaaatgt tccactaaaa acaccatgtc ttctggtgtt gtttttcgta
     7501 accgaaagta tttactaatc cggttataca ataaattctc caatcgctta tatcgttgaa
     7561 ttttttcatt tgggatggtt tcaaagtctc caaacaaacg ggcattggat cggtgaaaaa
     7621 aatcttttgt cgcaatacga aattctttca tccaatgagc ttctttggga gcactttctt
     7681 ctaataccag ctcaatccca acataccaac aaacattttg aatatatttt ccatgtagct
     7741 cttctaaaat gtgctgaaca ccttctaaat gttggtaggc aatcgttttt aattcccctt
     7801 cagaacgcac caattctttt gaggctttaa tcgttttttc aattttttct tcgtttgtac
     7861 aaaaaaagaa ccgaacatgc gaagaataac tttgacgaat cagacgcatg attgcttgtt
     7921 ggagttgata cgcctgatct tctcccacga agctatatga atacattggg aattcataat
     7981 aagcgtacac acgatcttct tctccaaaaa ctaaattgtc ttctaaataa taaaaaggaa
     8041 tcttaggcac tagttttggt agtcgcatcc ttttcacctc cataacgtac tacacaacga
     8101 attcctcgtt tgatttcttt ttttctattg gctttaccca ttggccatga aaggattctt
     8161 ttgaacgaca ctgaaataaa agaaaagaag ccaaataatt ataaggcttc cttccttgaa
     8221 attgttttcg tgtcattaaa aaagcaaata accacggaca aaaaaattgt cgagtaatcg
     8281 tatgttccat aacaaatgga aaaattcgaa aaatcccccc aaaaaagcca atcccaaacc
     8341 atatcgcaaa tgtttgtgtt acgggacgag gtaacgcgac cccatcccac aatttataaa
     8401 acactcgctc tatataccaa atctttgtcc aattgcgtat tttgttcatt ttatcgcccc
     8461 catgaccgca tacgatccaa aatccaattg aatacgccag acgtatctcc aactaaccaa
     8521 acagctactg ctagcacaag aatcaatgga atggctttgc ttactttccg ttcaaaggca
     8581 atatatgccg caccaccaag catcgccaac ataatcaaat acttaaaatc cccaacaaaa
     8641 tcagtaaaca cattttgaaa aaaattcatt tgctttttcc tccctgttaa tactcattga
     8701 actaatgacg atacacaata ccacagagca aaactgataa aaatttcact tgatattcct
     8761 cattatgcat tttttccgtt ccttgattgc tgttttttac gtaagaagta taaataattt
     8821 ctcacacgac gatacattac ttttacagga acatgcgtac aataaatccg gtgctgcgta
     8881 cttttctttg ttttaggatc aagtttcata gaatagaaat cataaaaata cttaccactt
     8941 tttttatcgt atgttccttc aatcaccagt gaaaagctag ggtggtaaat ctaataaata
     9001 caattgatac ttccctctac tagataaagg gataaaacca agtaccttta aatgatctga
     9061 tacagtcata aacacgatct atctcctccc aaataaaaca aaaaaaggct gtaatcgcat
     9121 tttgattaca gtcttttttg ttctcttttt ataaatgtac taattaaagg tttacttccg
     9181 gtctttttat ttaataaatc aattttccca aattattttc tttagcttta ttaacgattt
     9241 ctgtcgcaac taccacatct aatacagccg aaccaactgt tttgaagatt gtgatttgtt
     9301 ctccacttgt ccgaccgatc aattcttcat tgatcagttg gcctagttcc ccatgataac
     9361 tgtctctttg aatataccca tcttgcagcg gtgaaatgaa gtccccagct tcttttagaa
     9421 caccgtccat ggtatcaaaa ataacaacat ctgcggcttt gattatttct cgtggaattt
     9481 cacacatttc cggtgtgtaa gcaccgactc cgttgatgtg cgcacctttt ttgacccact
     9541 cagcagagaa tgtcgcacgt ttggaagtgg tcacacttgt aatgatatct gctccttcaa
     9601 cgcattcttg actggttttt gttgggcgca tcgttacaga aaactgttgc atcatttctt
     9661 cagcaaatcg gctggcacgg tcaaaatcaa tatcaaaaat ccgaacttct tctaatttac
     9721 gaacggtcaa catcgcttct aattgcgact gtgcttgtcc gccggttccg atcagtgcgc
     9781 cgattttagc atcttctctt gcaagaagtt ctgttgctgc accttgaaca gcgccggttc
     9841 ttaattgtgt caaataggta ccatccaaac aagcggatac catacccgtt tctggatcaa
     9901 ggacgatcat cgttgctggt acacttggta agtttttctt gatgttttca ggatacacgg
     9961 aaacgatttt gacacctaac gcaccttcgc caccttctac ataagctggc atatataagc
    10021 tttgtccgtt gctttttggc acatcgatat tggtgcgaag aggcacactt gcctttcctg
    10081 ctgaatacaa tgacaatgct tttttgtctg catcgatggc ttctctcatt gagaaacttt
    10141 tgataatatc ttctttttta agaaataaca tttgaatcgc tccttaatta gctgctgaaa
    10201 ctttttttaa ttgactgccc aacattgtat atgcactgta tagtaagacc aaaattacga
    10261 tccatttcaa gacataggta tctaaagatt tgactaggaa tacagcgacc aataccccca
    10321 atacaccgaa ggttgaagtg aaaagtgtga tttttctgct gtattcatta aatttaatga
    10381 attgaacact accgatcggt actgagaatg tacaagcacc catcatgata ggaaaggctg
    10441 ccactggatt caagcctagc aagtagacag ttaccatcgt taacgcataa gaaccaatgc
    10501 cgatattgtt caatgcgcca tagacaaaga gtaaaaatcc tgccaagatt aattttccgc
    10561 catacaattc actggctgtt ccgtttgatg gaatccagtt catttgtcct gcaaaaatca
    10621 ataatgcagc aatgaccaat cctatgccga caaacacttt gatcgttttc tctggtaact
    10681 tcacgacaaa tcttggtcct aaatatgcac cgataatttg acagataata caaacagcta
    10741 gtgtcttgat ccctacgttg atggcagtga tataagacaa tgccatgacg gctaccggta
    10801 cgacacattg ggtattcaac gttccgggta gttttttcat gctgacccat ttcaactttg
    10861 gataaagaac ggttccgata gcaaagtcgg aaacgccaaa tgttgaaaga aagaagatga
    10921 taaatgacgt gaagggcaac agacgcatat ccccaggttc ttctttgaaa ttttgtttgt
    10981 ttttgaaaag gtctcttaga aagtaaataa taaaatatag attgattgcg acaattagtg
    11041 tcagcaaaat attccgaatc ctaaaaattt cctcctacta gtaaatcttt ctgattccct
    11101 caaagtcctc accatatttt gctttaagtt cttcagaaga ttctgcaatc acttttagtg
    11161 tatatgcatc atgtttgaca tattcttttg ctagattgac catggcgtgg ttttttgtcc
    11221 aagtgacatc ggttgattgc agttcttcgt taatggtgcc aaacacgact tcttgctcat
    11281 cggcgacgat gtttatccat cttgctccaa agtcagcaag tttgtctgtt tcaaagccat
    11341 gaatataata tttttctaag ggaagatcat attcatgggt agaactgaat aaaattagta
    11401 caaacttatc aactcttttt tcagcttgct gcaatgtctt tagcagtgaa tccgtgaggt
    11461 tctcatgcca aacttgaatg agcaaacttt cttttgcgtt tttcaccaaa tgctctactt
    11521 tatccagaac atactcgatg ccatcaactt tccacagcat ttcttcttca tctttttctt
    11581 tgatcatccc tagattcttg gtaagaaaag aaagatcatt tttaacactt ttttctaatt
    11641 tctctaaaaa ctcatttgcc gagatggcat gataaagttt aggttctgat ttattaacga
    11701 caatcaaatt cttttttaat agtttttcca aaatgttata tactttcgag cgtggcacac
    11761 ctgagatttt acttacttca tatcctgtaa gattaccttt ttctaatagt gtggtataca
    11821 ctcttgtttc catctctgaa aaatcatatt ttttcatgat ttgaataatc gtttccattg
    11881 attaatcctc actttatttt tagtagctac tacggtaaca actataaatt ctttttgatg
    11941 atctg
//
LOCUS       DAXWLH010000077        11811 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXWLH010000077 DAXWLH010000000
VERSION     DAXWLH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.077"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1964
                     /locus_tag="ACGE36_001639"
     CDS             <1..1964
                     /locus_tag="ACGE36_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8384029.1"
                     /translation="KAKRGAAIVGTAMVGCSVAAPLVQPVPVQADETPVQFGARINTA
                     AFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSALSQAPYYNLFGIKGSYQGQT
                     VYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRNTSFGNGYYYAGTWKSNTKS
                     YTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPASGNAGGGATTGGNNTGSAGS
                     TGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITMDQLIQWNNIQNNFIYPGQK
                     LTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKYTVQSGDSVWKISNDHGITM
                     AQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTGNTSGSSSSSSSSSNTASGT
                     KYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQQLTVSNSGSTNSTSGNTGS
                     STNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGITMDQLIEWNNIKNNFIYPGQ
                     QLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGNTMYTVKAGESVWSVANKHH
                     ITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVTGSKTYTVKSGESVWSVADS
                     HGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2034..2240
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2391..4805
                     /gene="leuS"
                     /locus_tag="ACGE36_001640"
     CDS             2391..4805
                     /gene="leuS"
                     /locus_tag="ACGE36_001640"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFF8384030.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5153..6706
                     /locus_tag="ACGE36_001641"
     CDS             5153..6706
                     /locus_tag="ACGE36_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFF8384031.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7087..8871)
                     /locus_tag="ACGE36_001642"
     CDS             complement(7087..8871)
                     /locus_tag="ACGE36_001642"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384032.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8886..10607)
                     /locus_tag="ACGE36_001643"
     CDS             complement(8886..10607)
                     /locus_tag="ACGE36_001643"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384033.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10707..11468
                     /locus_tag="ACGE36_001644"
     CDS             10707..11468
                     /locus_tag="ACGE36_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8384034.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3853 a         2148 c         2398 g         3412 t
ORIGIN      
        1 gaaaagcaaa acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg
       61 ccccattagt acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa
      121 gaatcaatac agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag
      181 ccaacgatct atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca
      241 gtagtgcgtt atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag
      301 gacaaaccgt ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg
      361 agcctttccg acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta
      421 gaaatacttc atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat
      481 cctatacaga tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag
      541 gcaaattaaa taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg
      601 gaaacgcagg cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg
      661 gcggttcaag caattctggt aatacaaact caggttcagc aacttcgacc acttatactg
      721 tccaatctgg tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat
      781 tgatccaatg gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca
      841 aaggtagcca ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag
      901 gaaatgctgg tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg
      961 gtgattctgt ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat
     1021 ggaacaatat caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg
     1081 gttcttctaa tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt
     1141 cgtccagctc aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag
     1201 actctgtttg gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga
     1261 acaatatcca aaataacttt atctacccag gacaacaact gactgtttca aacagtggat
     1321 caacaaacag cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag
     1381 ttaatactgg cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt
     1441 ggagcgtggc aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca
     1501 aaaacaactt catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata
     1561 caaatacagg ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga
     1621 caagtacatc atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga
     1681 gcgtagcgaa caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa
     1741 ataatttcat ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa
     1801 accaacaatc agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga
     1861 gtgttgctga cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga
     1921 acaactttat ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga
     1981 aataacgatt gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca
     2041 cttgaaaagg aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt
     2101 gcacggatgc ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc
     2161 ggcggtgacg gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc
     2221 gcgtagatta cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt
     2281 gaagtttgag aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg
     2341 ttatatttat aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca
     2401 atcataaaga aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca
     2461 cccatgatga tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg
     2521 gtcaagggct acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg
     2581 tgaaaagaag tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc
     2641 ctgctgaaca atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata
     2701 tcgaaacatt ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag
     2761 tgaatacaac tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg
     2821 aaaaaggtct agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag
     2881 ttatttcaaa tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca
     2941 gaaaaccaat gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg
     3001 atctagaatt agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc
     3061 gttcagtagg agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat
     3121 ttacgacacg accagatact ctattcggtg caacttactc ggttcttgca cctgaactag
     3181 atcttgttag agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag
     3241 agacagctaa aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat
     3301 ttactggtgc ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg
     3361 attatgtatt atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac
     3421 gtgactttga attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag
     3481 atgttgaaaa agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg
     3541 gcatgaacaa acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag
     3601 ggaaaaaaga agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg
     3661 gtgaaccaat cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag
     3721 agctaccatt acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc
     3781 cattagcaaa tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc
     3841 gtcgcgaaac gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata
     3901 tcgaccctca taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag
     3961 ttgacatcta cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct
     4021 ggcataaatt cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat
     4081 acaatcaagg aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg
     4141 tagtcaatcc tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa
     4201 tgttcatggg gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc
     4261 gtaaattctt ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc
     4321 gtatcactac attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag
     4381 tgacagaaga ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta
     4441 tcaatgaagc ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac
     4501 tactcgcacc gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg
     4561 gtggtatttc ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag
     4621 tagaagttgt tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact
     4681 taggaaaaga tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg
     4741 aaggcaaaac agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta
     4801 attaattggc tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga
     4861 atgaacttca ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa
     4921 aaggttttga ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg
     4981 taagagatct ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata
     5041 aactacctga aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg
     5101 attgtatgga ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca
     5161 agaactattt attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa
     5221 aagagcgaaa agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt
     5281 tttcggagct gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg
     5341 gatgcaaaaa ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt
     5401 tgtgcttatt gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt
     5461 cgatcaatgg cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga
     5521 tgatgactat ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag
     5581 tgattgccag ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa
     5641 atcaaaattc ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga
     5701 aaaaagccag tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg
     5761 ttttttcctc attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat
     5821 gtcttctcat ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat
     5881 acaaaagaca aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca
     5941 aaagataaat gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag
     6001 tggtgctaat tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac
     6061 cctgacaacg gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc
     6121 cgatttctca tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga
     6181 taatggtaaa acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg
     6241 aaaagcggta ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc
     6301 tgcagaaact ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg
     6361 aagtacaatg atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt
     6421 tgtttcgaca cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc
     6481 agttctgtcg attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa
     6541 tgaagttact caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg
     6601 accaatcgat gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac
     6661 atggcaattc gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt
     6721 gagcctgacg ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt
     6781 gttgaatagt gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt
     6841 atagcttctc gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc
     6901 gtttattcgt aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga
     6961 aggagctatc caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt
     7021 acatttaaaa aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa
     7081 tgtttgttac gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg
     7141 gagtagttca gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat
     7201 gtctgcattt tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt
     7261 cacagcttcc attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc
     7321 ttcatctaaa ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg
     7381 ttgctgtcct tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg
     7441 cagtgttcga ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct
     7501 tgtggcatcc attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt
     7561 attttgtaaa accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc
     7621 aagaccgtcg aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac
     7681 taaagtcgtt tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac
     7741 tgaaaaatcg acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt
     7801 gaaagctatt tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc
     7861 atctgtttca tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga
     7921 gttgatcaca taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata
     7981 agcttgtaag aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc
     8041 acttatgatg aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat
     8101 aaattgagca cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc
     8161 tgcatgagct tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat
     8221 tcgattattt aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt
     8281 cgcaatctta gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag
     8341 cttgctttcg atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa
     8401 aaattgattg atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg
     8461 actaatgatg tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt
     8521 ttttgtgatt tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg
     8581 ttcctgaata aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg
     8641 gaacaacaat gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat
     8701 ttctgataag agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag
     8761 gttcccgaat aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat
     8821 tttccaaaaa cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct
     8881 tctttctata ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt
     8941 tcttcatgag taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct
     9001 tgttggattg tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta
     9061 tgaagggctg ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg
     9121 aatacataga tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg
     9181 ccccctgaat aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg
     9241 tccactagtt cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca
     9301 tattcatttc ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga
     9361 gggacataac tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca
     9421 ccaagggtta cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta
     9481 ctttttccag atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa
     9541 tcaatatctt ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa
     9601 gcagctgcat ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca
     9661 atttcgatct ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg
     9721 ataatgacca ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg
     9781 actacagcag tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac
     9841 cacaaaatag aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca
     9901 aatattcggt tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc
     9961 ttttcttcat atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct
    10021 ctcaatgtga tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct
    10081 cgtttcatca gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat
    10141 gttagtaata gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct
    10201 ggtaagatca tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc
    10261 attcttgtca aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg
    10321 aatttacgga aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa
    10381 aagtaactag aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact
    10441 ccaatttttt ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct
    10501 actaattttg gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg
    10561 agagcgagtc ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt
    10621 ttttcctgat acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga
    10681 ataaactgaa aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg
    10741 aactatttga tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt
    10801 caagtagaca agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt
    10861 atgatatcgt tttttataga aaattagata taccgatcaa acagatgaag aatttatatg
    10921 gaaaaacatt gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag
    10981 aactggtggt gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat
    11041 tgatgatcga tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga
    11101 tctcaacgga gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct
    11161 ttgggatgat gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc
    11221 ctagtgaagt acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt
    11281 atcgtcgatt cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa
    11341 tcagagaaag aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat
    11401 tgacgaatac ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga
    11461 aaaaatagga aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga
    11521 aaaattttct ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga
    11581 attttagttt tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat
    11641 atatacgatg taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg
    11701 tataattcat ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat
    11761 tgtatcagat gagaatgata aattaaatag gtgcataaaa gcattacact t
//
LOCUS       DAXWLH010000078        11624 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXWLH010000078 DAXWLH010000000
VERSION     DAXWLH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.078"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            39..410
                     /locus_tag="ACGE36_001645"
     CDS             39..410
                     /locus_tag="ACGE36_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384035.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            495..695
                     /locus_tag="ACGE36_001646"
     CDS             495..695
                     /locus_tag="ACGE36_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384036.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            829..2055
                     /locus_tag="ACGE36_001647"
     CDS             829..2055
                     /locus_tag="ACGE36_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384037.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2652..3047
                     /locus_tag="ACGE36_001648"
                     /pseudo
     CDS             2652..3047
                     /locus_tag="ACGE36_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3125..4164
                     /locus_tag="ACGE36_001649"
     CDS             join(3125..3622,3625..4164)
                     /locus_tag="ACGE36_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFF8384038.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            4177..5115
                     /locus_tag="ACGE36_001650"
                     /pseudo
     CDS             4177..5115
                     /locus_tag="ACGE36_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            5108..5455
                     /locus_tag="ACGE36_001651"
     CDS             5108..5455
                     /locus_tag="ACGE36_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384039.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            5452..5634
                     /locus_tag="ACGE36_001652"
     CDS             5452..5634
                     /locus_tag="ACGE36_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384040.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            5770..6627
                     /locus_tag="ACGE36_001653"
     CDS             5770..6627
                     /locus_tag="ACGE36_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8384041.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            6647..7207
                     /locus_tag="ACGE36_001654"
     CDS             6647..7207
                     /locus_tag="ACGE36_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFF8384042.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            8096..8863
                     /locus_tag="ACGE36_001655"
     CDS             8096..8863
                     /locus_tag="ACGE36_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFF8384043.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            8889..9434
                     /locus_tag="ACGE36_001656"
     CDS             8889..9434
                     /locus_tag="ACGE36_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384044.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYVHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            9435..10001
                     /locus_tag="ACGE36_001657"
     CDS             9435..10001
                     /locus_tag="ACGE36_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384045.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            10016..10264
                     /locus_tag="ACGE36_001658"
     CDS             10016..10264
                     /locus_tag="ACGE36_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384046.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANGFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            10284..10523
                     /locus_tag="ACGE36_001659"
     CDS             10284..10523
                     /locus_tag="ACGE36_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384047.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            10894..11175
                     /locus_tag="ACGE36_001660"
     CDS             10894..11175
                     /locus_tag="ACGE36_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8384048.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            11165..11506
                     /locus_tag="ACGE36_001661"
     CDS             11165..11506
                     /locus_tag="ACGE36_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8384049.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKTGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         4334 a         1618 c         2304 g         3368 t
ORIGIN      
        1 taaataggac acaaaatttg gaaagtaggt aagtcactat gagagaacta actttatctt
       61 ctaaggaatt agaaaagaaa actgctttat taaacgaaat atttagtgct gattgtataa
      121 tccaaagttt aaaagaaagt tcattttcaa aactaacaga tcaggaaaaa atttcttata
      181 tgctaagttt agataatcta ggaactaaat atgatgaatc agcaagtagc gataagcttt
      241 tgattgaatt agtatcaagt cttagtagtt ttggaaaaat gaaaagattt aatgtgatat
      301 tagaaatttt tgcttggtta atcccttcat ttatttggtt tttttatatt ttcatagttg
      361 aatctgagtt aatagccaag gtctctcttg gactgttaat tccgatataa ataggttcag
      421 caatttactt taataacttg tatacaccta ttcaaataaa aaagcagtta ttacatgaag
      481 gattaagtgg taatatgtta aaaagaattg catgggataa agataataaa acagatattg
      541 agcttaaaga atataaatac aattatttct cgagatgtat agatagaaag ttactgttta
      601 aaaaagaagg attagaattt agttttgatg aagatgaatt tgaaaaatta gtgatggact
      661 ggcgtaagaa tttactggat acggtacaat tctaaaagca attgaggtcg aaattccgat
      721 acttgaatca tgatctatat tatcgtgtta tgagtgaaat ttctaataaa gataagatat
      781 ttcatttgga cagtcatgtg aatatgagct taacaattaa acgtatttat gatcttttat
      841 atgtttataa acattaatga ggtgctaaaa ttgacaacaa taactatgac aaaaaaaata
      901 gaagtatatg ttgatggatc acaagaagaa agggattcct tttttgattt tttttggaaa
      961 aatagtagag aatatgtaaa agcgttgaat ctggtatata aacagctaat gtttgaagag
     1021 ttgataaaag atgcaattaa agaaatggat caaggttatg ctgagagaga agaaagtatt
     1081 caaaatcgga ttgaaaagtt agcatatact gatccaatag atcaaaaaaa acttgataaa
     1141 gcaaaagctg aattatctaa attgagaaat gaaaaaaata aagaagcgag agaaatatta
     1201 acgcaagcga tagggttgaa gagtcaaacg agaacacgag atgtagcaaa aaaattagac
     1261 tttgactttg gagattttgt tgattccgca aatatgaaag ctagtaaaga ttttggcaat
     1321 gattttatcg gaattgtgac cggacaaaga acgcctagaa tatataaaaa tagaaacaaa
     1381 tatggattat taccttttag aggtcgagat attaaaatcc ataaagatga taagttttat
     1441 atttatttac ctaaaggctt taggtttaaa gtgaatttag gtagcaagcc aagaaaagca
     1501 agagatgttg cttcagtttt acaacacatt atggatggcg attacaaatt aaatcaaagt
     1561 aatcttattt ttgatggaag aaaattattt tttaatctaa caatttcttt tgaaaaaagc
     1621 tataatgaaa tggaattcaa gaaaggtaat gaattgatcg taaactttgc agatttgtgt
     1681 agcggtgaat gtttaattaa tgataaatct gtaatgtctt ttggtgatag ccaatatata
     1741 agaaacttca aaagtaaaat tttagcaatg aaatcaaaag aacaaagccg aggagtcttt
     1801 tctaagggcg gtcatggtcg aaaaagaaaa ttagatcaag atcgatggga agcaataaag
     1861 aaaagagaaa gtaattttgt taaaacttat aataatcaat gttctgcaaa aatcgttaaa
     1921 actgctattc gcaataaagc tcaaattatt cgtatcattg aaccagataa ggcttatggc
     1981 gaatgggctt actatcaatt gaaagaacaa ctagtaaaca aaggaaaaag atatgggata
     2041 gaagtcagaa gttagtaaat taaacaatcg ttgatttggc aacgaaatag tgagggaaaa
     2101 tccactcact gaagggtaaa ctccgtcaag ttgtgtgacc agacggaagg acgaatgttg
     2161 ttgaggtttt agaaccatat aaaacagcat gtttctcaaa tttttaggat aagggaaatt
     2221 gctagcagtt tgttttagaa ccatataaaa cagcatgttt ctcaaatgag tactttaaat
     2281 taattgatgg cgttcctgct ttagaaccat ataaaacaac atggatttaa aacaagaagt
     2341 aagtcagttc tccttttact ttagtaagga aaaataaagt attctcaata aaacttaaaa
     2401 taagcaaacc tttgttttaa aatgattgtc aagttaaaaa aatctgggat catataaaat
     2461 agtatagttc tcaaatatga ttagaaaccc tattaaagag caatattaag ccaacttact
     2521 taaatcgttt aattaagaac aataatctct tttagtcaga cttttagtct gacttttttg
     2581 tatttccaaa atttattctt tacaaaaacg aacatacgtt tgtataatta cttgtgaatg
     2641 ggggaataaa aatgtttgat tacagtagag aaccggtgaa tgactatttt ttaatcgaca
     2701 tgaagtcttt ctatgctagc gtggaatgta ttgaacgaaa tttagaccca atgactacac
     2761 cgttaattgt catgagtaga tcggacaaca ctggttcagg tctgactcta gcttcttcac
     2821 ctactgcaaa gaaaaaatat catattacta atgttagtag agctactgat ttaccttatc
     2881 cattaccaaa agaacttcat attgttccac cacgaatgaa cttatatata aaaaaaaata
     2941 tggaagttaa tcagattttt agaaagtatg tcgcagatga agatttattg atttattcga
     3001 ttgatgaatc aattttaaaa gtgacaaaat ccttaaattt atttactata taaattgagt
     3061 taaattgtat tcgtttttcc tcctataggc gtatactaaa gaaaacactt gaggagtggg
     3121 gaacatggaa acagaacgcc aaatccgtga attggaaaaa gcgatgaata cgacagacaa
     3181 ccgccggttg tacgagcgtt tcctggcagt caagttggtt cttgaaggcc acacccggaa
     3241 agaagccggg cacaccatcg gccgcgacga gcatactgtc agccactatg tgaaaaacta
     3301 ctgccataat ggaatccaag gtttagtctc caaaaaacaa agcggacgac cttcacggct
     3361 gacagatgaa caggaagcgg aactggtcca aatcgtcgcc taccacactc cggaggaagt
     3421 aggtttcaaa tcccgtgcca attggacatt ggcgattgtg tgtgacctga ttctccggga
     3481 atggagttat ggatatacgc aaaaaggcgt ggcggacctc cttcaccggc taggactaag
     3541 ctggacccgt ccgacgtata ccctcaaaaa agcggattcc aagaaacaac aggtattttt
     3601 ggaagaaacc ttcccgagcc taaaaaaaaa ttgctgaata gcgatattga ccacctccta
     3661 ttcgaggacg aaaccatgat ccgggattac caagccattg gctgcacctg gtttctccgc
     3721 ggccaccagc gaaaaatccc cacttatgga caacacaaag gggtaaaatt gataggcacc
     3781 ttggattatg aaaccggtcg gatcatttgt acagaagaag gggcatacga cgcagcggcc
     3841 ttcctccgtt ttctggagcg gacgttgcgg gaatacccca ccggaaaaat cgtgatggta
     3901 ttggataatg cccttatcca ccacgccaag ctgatccagt cttttcttga agaaaacgct
     3961 aggcgcctgg cactcatctt cctccctccc tacagccctc agttgaacct aatggaaggc
     4021 gtttggaagt ggctcaagga atcggtcatc aacaatgttt tttttgacca cgtccagaaa
     4081 atcaaacagt cggtccgcgg tttcttagcc gatgtaaacg agcgtcccct tgaggtgatt
     4141 gaccgactgt gcgtgcggat gtaaagtatt taattcaacc tatatagttc aggaacaaga
     4201 agcgagagaa gaagagaatt agctaaaata atcaaagatg agattcagaa agaaacaggt
     4261 ctaatagcaa cagtcggaat aggagataac ccattattag ctaaacttgc actggacaat
     4321 gaagcgaaga gtagtaaaga atttatagct gagtggactt atgagactgt acctgaaaaa
     4381 atatggaaaa taaacgagat gactgacttt tggggcattg gatcacgcat gaaaaaaaga
     4441 ttgaatcata tgggaattat gagtatagaa gatttagcaa attggaatcc ctacacaatt
     4501 aaaagtcagt taggtgtaat cggactccag ttatatttcc ataccaatgg aattgatcgt
     4561 acggatattg ctattccacc agaaccaacc aaagaaaagt cttatgggaa ttcacaagtt
     4621 ctaccaaaag attatacgag gagagaagaa gtagaactag tagtcaagga gatggcagag
     4681 caagtagcca ttcgaattag acaacacaat tgcaaaactg gttgtgtacg cttagcaatt
     4741 ggtacgtcaa ttttggaaga aaaatcaggt ttctcgcacc agatgaaaat accaattaca
     4801 gataatacga aagaattaca aaattattgt ctttttttat tcaataagtt ttatgagggg
     4861 caggaagtac gacatatagg agtaacttat agcagattag tatacacaga ttccctacaa
     4921 ttagatttat ttagtgatcc aagaaaaaag attgctgaag aaaatttgga taggattatt
     4981 gataagatta gacaaaaata tggatttaca gctattatac atgcttcatc tatgttagaa
     5041 ggtgcaagaa gtattacgag aagcacttta ataggaggac acgcaggagg attaggaggt
     5101 ttgcataatg attgaatata acgatagagg acgtttaaaa tggtcgggat tttatttgtc
     5161 tgatcatact gaaagaatca atgcagacaa tgtacaacgt gctaatcata atttggcaaa
     5221 agaacaaatg acgactgaag agattgcgaa aatactgaga actgctgtca taaaaaatag
     5281 acccgtctgc attcaaaaag aggaacgaaa cgctgaaggt tactatccag atgatgtagt
     5341 aggaaaaatt attggatatg acgagcttgg tgtatttgtg gaagaagaaa aagttcacta
     5401 tgatgaaatt aggaatgttt ctttctcgga attattaaaa tggagtcaga tatgaacatt
     5461 attaaaaaat acgaattcgg ttatattacg tatgaacagc taatagagga attatgggga
     5521 tatggtcaac aattaattaa tgaagtgggg attgattgct tttgtttcta tattgaatcc
     5581 ggagcaggat accataagaa cagatactat atatttccat atccaccaaa atagtctctt
     5641 agatattttt aaattttctc caaatacttt caattattgt gaaatatttc cctttaatta
     5701 atcaaatttt ttataaaagt aatatataaa ataaattgct tgaagtatta tatttagata
     5761 tcataattta tggaagaaaa attaattaaa aaggatgaga ttataatggg gttactggtt
     5821 ccgagagcta atacttatac tataccttta acgaaacgcc aattaattgc taaaagatat
     5881 caaaggatta ctagagccat caatagagag ttttggaatt ctgaaagtga tactgctcac
     5941 agtttatacg taggttctta tgggagagga acggcaatta gtactagtga tatagatata
     6001 atagttgaat taccgatggc agaatttgac agattcaaaa attatttatc aaatggcccg
     6061 tctaagttat tacaagttat aaaaaacgca tttcaggaaa tactaccaaa tagtgatatt
     6121 agagctgatg gacaagttgt taaaataaat ttccatgatg gaatcaaatt tgaaatagtt
     6181 ccagctttta atgaaaaaga ttattggggt gagtcaaaag ggtttattta tccagattca
     6241 aacatgggag ggaactggaa agctacaaat cccaaaaaag agcaagaagc catgaaatta
     6301 aagaatacaa aaagtaataa tttattatat gctacgtgta aacattttcg acatgtacga
     6361 gatacagaat ttacgagtta tcatctttca ggaatagtaa ttgacagttt cgtatatgag
     6421 gctatgggaa attggaaatt cgttgaaaat aacagtgggg gtcaaaacat tagttctgta
     6481 tcttatgaga cagctttatt agaatactat aactcacata aagttatggg cggtttaaat
     6541 ttatactctc ctggaagcaa tcaatttgtt aattctgata gtagtatcat atgtttagaa
     6601 aaagtattaa aaaaaatagc tttataaagt tttatggagg aacaacatgg gagacgaaaa
     6661 aaagcgcttg gaagttcaat tacgtgaagc atatggacga gtaacgtata cttacaccac
     6721 tcatttaaag caagccgata atattatttc tgacaataaa attttaaggt gtcttcaaat
     6781 tggtttgtca gcccttacta ctggaggggt acttagtacc attattactg ataatagtga
     6841 gttaaccttt gttgctggat tatgttcaac aatttctctt ggattaaact tatactttaa
     6901 gaattttgat cttgctgaag aagctaaaaa tcatcaaatt gttgccgata aattatggtt
     6961 gatcagagaa gaatacgtaa gcttactaac agatttaaat cgtttaagtg ttgaagatat
     7021 tgtgaaaaaa agagatcaat taatgaacga aactcatgaa gcatataaaa atgcccctaa
     7081 aacaaatagt aaaagctatg aatcagctca gaaagcatta aaatctgaag aagagcaatt
     7141 ttttacagaa gaggaattgg ataagatgtt accagagcat cttagaaaaa agagttctaa
     7201 atattgaact ctttttttat gcttaaaatt ctcttgtaat ttttctattc atctatattt
     7261 aagaagaaat atgatgatag aaacatctcc taaagccacc gcaagccgtt ctaagcatag
     7321 cttagcccga cattgctaga tatgctcatc tcaccatgaa cgtttcctaa agccaccgca
     7381 agccgtccta gccattgcct agtccgacat cgctagatta gtgcatctcg ccataaaagt
     7441 tttctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct
     7501 catctcacca tgaacgtttc ctaaagccac cgcaaaccgt cctagccatc gcctagtccg
     7561 attttgcttg atcggttcca tttctgttta tttagtgggt caacaccgta atcttaaata
     7621 gccatcgcct atttaagatt actaggtctg ctttgaaggt aaaaagtctt tcattcttca
     7681 agacttttag gctttcaagc tacgcctgaa agcattgaaa ttcatcgctc tttatttggt
     7741 tttgccattt ataattcctt acgcccatta tctttttcgc ctgtcatacc ttgtcagaag
     7801 tgtttagagc ttatctcatc tttggattaa tacaagtggc gtaaattttt ttctaaacgc
     7861 aagcgttacg aaaataattt acttaaacac ttgtattaat ccggcagata agaacgcatt
     7921 ctaatcactc ccgacaaggc aagacaggcg aaaaagataa ataaatgaac gaaaggaaga
     7981 aacataaaat gcaaaaccaa atagcgatga agaaaaaacc acctccctct aaaacaatcc
     8041 aaacaaaaaa gaattttgaa aaaccaaaaa tcaaaaacag aaagaagcgt tcaacatgag
     8101 agatagaatt ttagaagtta atcaagaaat ggtaaacaag ttaacaaata tcagtgggaa
     8161 ctatgaaaag tacgatggta ctttacttga taactatatt tttcacgata cacaaaatat
     8221 cagaattggc agaagtaaac cgagagcgtt cattctcatc aaagagaaat atttgaatga
     8281 atggaatagc acctatgaaa tggttatgac tgataacttt gaaaaggtat tggatttcag
     8341 aaatgctttg ttgggttctg atatgggaaa tagcagtctt gaatctattg aagttgaaaa
     8401 caagaccgag aatgaaggtg aaggattgat taagaatgtc ttgaaaatcg aacaaaaata
     8461 tatcagtgaa gatagaagtt tttatagagt cacaagctcg gttggtctag caaactttgc
     8521 tactaactta attggaaaca atgctagtga atctttgcta gtgatgtgtc tgaatacaaa
     8581 gaatgaggtc attgcttata gcgaagtttt caaaggtgga attaatcaat cagtagcgat
     8641 accaagagag atttttcaac tagctttgct aaacaattca gcaaaaatcg cagtatcaca
     8701 taatcatccg agcggatatg aatttccaag tgaaaatgac aaaagtttta ctacgaattt
     8761 gaaaaacgca ggaaaaacaa ttggtattga attgttagac catatcatcg taacaagtaa
     8821 aaacacaagc tattactcat ttcaagaaaa tggttcttta taaaaattag agaaaagagg
     8881 aaaacaagat gggcgaaaga acacaagcgt taattacagt caaatatggt aacagcaaaa
     8941 cgaaagcaag cgtgtcgatt cattatcagt gggggtatgg tcgatatatg ctgatggacg
     9001 tattgaatca aggaatttat ttaatcaata aagcatacga cttacgagat aaatcaagag
     9061 aagaattagc aaaaggaatc tttgatagaa gtacaggagt tatcaatttt gttacctatg
     9121 aatctggagt gagtgcttat gatgaaacag ggtttggtgg gcgctatgaa ccttatcaag
     9181 aaacgattga agatgtgatg aagttttcag ataacaatga tggctatgtt catttagaga
     9241 tggtgttcga tgaaaatgga tttagtctag atgataaaac cagattgcga ttgtttgatt
     9301 ttgagggtca acagatagga ctaaacaagt tttgtgaaag cggaaaagag tttgctacga
     9361 aagattttat taagggatac agattattgc tagaaaatta tggaataaac ctagaagaag
     9421 agagcgtgaa ataagtgttt gaaaaaagaa aagaccagtt ccctaattta gaagcgaaaa
     9481 gtcaaaaagt gattgatggt gaaatgattg aattattcca aaaaagttat ggaaccatag
     9541 aaactaccgc aagttgcaaa tactactttc aaggaaagag aaacacgttt aaaggctttg
     9601 ccgattctga tgaagaattg gcaaagaaaa tcattgattg gcttgtcagc agtgaacacg
     9661 tagcaaggga agataccgtt agaacaacga ttaatgggga agtcctaaca ggtactagat
     9721 gtgtttgttg taatcatgaa tttgtagaag atgaaggttg ctaccgaatc aatgaatatg
     9781 gtgattttat ttgccagagt tgccacgaga tagaatttcc aaccgaaaga agttggtttg
     9841 aatacatgct acaagattat gagatggaaa aagaaaatgg tgaagtagtt ttttgtacca
     9901 aagaggaatt gagtaagaga agcgatgatg aaattgaaga ttggtgtgtg tggttagtag
     9961 gggcagatga ttgcgttgca tattacacaa cagcagaata ggagtgtgaa tcagcatgga
    10021 aggaatggat tttctagatc atgaagattt ggttgatttt ggttatacat ggaaaggaat
    10081 ggtaggtatt agcagaagtc ttgctaatgg cttttacgaa agaaattatg cagtatatgt
    10141 tctttatgat gacgatacag aaagccttgt tgacgaggaa tataaattgg atttagagaa
    10201 tgttttatat ggaatagaaa aggaagattt agcaaaatat attttttcat ggttgggtca
    10261 ataagtgaaa ggaagataga aacatgacaa aatttaaaag taagggtatg caatggtgtt
    10321 cttattatta tgttggaaat gatgaagatt tattgagaaa aacccaacga aaaggttatc
    10381 cagataagat attgaaacaa ataggttatt acatgccaga agagaggata gaaaaagaat
    10441 tttgtgaggt tctttttgat agtgaacagc tattagtttt gttaacccaa aataaattga
    10501 tttgccaatt tgaaaataat taagatgagg tggtgaacca tgaatgaaga taagatggta
    10561 ttaagtgaat atgctagatt gagaactatt aattctttag catctcattt aagttgttct
    10621 ttcctaacaa aggtgctaaa attaatttac aaatgtgaag attttgtaca agagaagcac
    10681 aaagacaagc ctactgcgaa taggcttgtc tttttttcat tcattacgat tccgtatgtg
    10741 tgggttagcg acaatttgtt caattaccgt atgatatagc atcttaattt gattagatta
    10801 cttgcaatgt agtttattaa gagtataata taatcataaa tgtggacaat ttgataaata
    10861 atgagtgttt tatactcaaa aggagtggat gaaatgaagg tcaaaaatcc aactgaaaaa
    10921 agcagaatcc aagtcggaat agacaagaat ttaaaagaga acgcagaaat gatattggaa
    10981 gagctaggtt taaatcctac aacagcaata acaattcttt acaaacaagt agtcgctaga
    11041 ggtgagtttc cagtagagat aaagctaagt gaagaggaga agcaaggaat cagattgcaa
    11101 cagctaacta aggacatgcc agtagatgtt ctagatacag atgaaaagtt agaagagtgg
    11161 ttcgatgaag cctaatgaga tagtaacggt atatgttgca tttacagata aaaatggtgg
    11221 taaaagaaga cctattttgg tggttagtga taaagaagat agagtagaat tctttggaat
    11281 cacaagtcaa tatgagaaaa aatcagacca aataaagagg gtgtattttc ctattgcaga
    11341 atgggaaaaa acaggactta aaaagcaatc atggattgac gtagggtctt taaaggctat
    11401 tcccaaaagc cgagagaaca tatcattcaa aaaggttggt atgttatcag ctagtgatac
    11461 gaaaaagcta aataggttta tcgagaaatt acaaagaaaa gtctaacaag gcttttcttt
    11521 tttgtttata tgtatattaa gatatacgtt tatacaaacg tatttatgta tatgaaaata
    11581 tacgtaaata tatacgtgat tataaacatt aagaaaacgt taag
//
LOCUS       DAXWLH010000079        11609 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXWLH010000079 DAXWLH010000000
VERSION     DAXWLH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.079"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(44..841)
                     /locus_tag="ACGE36_001662"
     CDS             complement(44..841)
                     /locus_tag="ACGE36_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFF8384050.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(838..2022)
                     /locus_tag="ACGE36_001663"
     CDS             complement(838..2022)
                     /locus_tag="ACGE36_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFF8384051.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2047..3294)
                     /locus_tag="ACGE36_001664"
     CDS             complement(2047..3294)
                     /locus_tag="ACGE36_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8384052.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE36_001665"
     CDS             3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE36_001665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFF8384053.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5274..5588
                     /locus_tag="ACGE36_001666"
     CDS             5274..5588
                     /locus_tag="ACGE36_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFF8384054.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5613..6209
                     /gene="recR"
                     /locus_tag="ACGE36_001667"
     CDS             5613..6209
                     /gene="recR"
                     /locus_tag="ACGE36_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFF8384055.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE36_001668"
     CDS             6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE36_001668"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFF8384056.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6932..7261
                     /locus_tag="ACGE36_001669"
     CDS             6932..7261
                     /locus_tag="ACGE36_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFF8384057.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7275..8213
                     /gene="holB"
                     /locus_tag="ACGE36_001670"
     CDS             7275..8213
                     /gene="holB"
                     /locus_tag="ACGE36_001670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFF8384058.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8249..9073
                     /locus_tag="ACGE36_001671"
     CDS             8249..9073
                     /locus_tag="ACGE36_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFF8384059.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9066..9413
                     /locus_tag="ACGE36_001672"
     CDS             9066..9413
                     /locus_tag="ACGE36_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFF8384060.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE36_001673"
     CDS             9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE36_001673"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFF8384061.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE36_001674"
     CDS             10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE36_001674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFF8384062.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
BASE COUNT         3714 a         2174 c         2512 g         3209 t
ORIGIN      
        1 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
       61 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
      121 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
      181 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
      241 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
      301 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
      361 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
      421 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
      481 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
      541 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
      601 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
      661 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
      721 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
      781 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaat tttttcatca
      841 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
      901 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
      961 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
     1021 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
     1081 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
     1141 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
     1201 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
     1261 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
     1321 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
     1381 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
     1441 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
     1501 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
     1561 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
     1621 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
     1681 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
     1741 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
     1801 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
     1861 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
     1921 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
     1981 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
     2041 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
     2101 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
     2161 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
     2221 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
     2281 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
     2341 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
     2401 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
     2461 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
     2521 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
     2581 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
     2641 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
     2701 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
     2761 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
     2821 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
     2881 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
     2941 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
     3001 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
     3061 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
     3121 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
     3181 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
     3241 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
     3301 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
     3361 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
     3421 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
     3481 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
     3541 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
     3601 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
     3661 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
     3721 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
     3781 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
     3841 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
     3901 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
     3961 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
     4021 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
     4081 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
     4141 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
     4201 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
     4261 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
     4321 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
     4381 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
     4441 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
     4501 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
     4561 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
     4621 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
     4681 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
     4741 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
     4801 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
     4861 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
     4921 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
     4981 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
     5041 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
     5101 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
     5161 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
     5221 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
     5281 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
     5341 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
     5401 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
     5461 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
     5521 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
     5581 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
     5641 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
     5701 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
     5761 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
     5821 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
     5881 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
     5941 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
     6001 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
     6061 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
     6121 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
     6181 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
     6241 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
     6301 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
     6361 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
     6421 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
     6481 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
     6541 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
     6601 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
     6661 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
     6721 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
     6781 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
     6841 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
     6901 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
     6961 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
     7021 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
     7081 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
     7141 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
     7201 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
     7261 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
     7321 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
     7381 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
     7441 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
     7501 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
     7561 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
     7621 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
     7681 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
     7741 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
     7801 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
     7861 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
     7921 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
     7981 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
     8041 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
     8101 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
     8161 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
     8221 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
     8281 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
     8341 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
     8401 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
     8461 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
     8521 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
     8581 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
     8641 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
     8701 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
     8761 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
     8821 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
     8881 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
     8941 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
     9001 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
     9061 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
     9121 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
     9181 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
     9241 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
     9301 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
     9361 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
     9421 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
     9481 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
     9541 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
     9601 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
     9661 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
     9721 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
     9781 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
     9841 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
     9901 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
     9961 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    10021 attgacgaat ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    10081 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    10141 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    10201 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    10261 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    10321 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    10381 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    10441 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    10501 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    10561 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    10621 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    10681 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    10741 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    10801 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    10861 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    10921 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    10981 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    11041 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    11101 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    11161 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    11221 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    11281 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    11341 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    11401 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    11461 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    11521 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    11581 ataaaaacag ctttgagagt tgaaaccgg
//
LOCUS       DAXWLH010000080        11357 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXWLH010000080 DAXWLH010000000
VERSION     DAXWLH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.080"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(274..2283)
                     /gene="metG"
                     /locus_tag="ACGE36_001675"
     CDS             complement(274..2283)
                     /gene="metG"
                     /locus_tag="ACGE36_001675"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFF8384063.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2551..2829
                     /locus_tag="ACGE36_001676"
     CDS             2551..2829
                     /locus_tag="ACGE36_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384064.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2926..3747
                     /locus_tag="ACGE36_001677"
     CDS             2926..3747
                     /locus_tag="ACGE36_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8384065.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4032..5042)
                     /locus_tag="ACGE36_001678"
     CDS             complement(4032..5042)
                     /locus_tag="ACGE36_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8384066.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5054..6007)
                     /locus_tag="ACGE36_001679"
     CDS             complement(5054..6007)
                     /locus_tag="ACGE36_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384067.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5997..7067)
                     /locus_tag="ACGE36_001680"
     CDS             complement(5997..7067)
                     /locus_tag="ACGE36_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384068.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7073..8116)
                     /locus_tag="ACGE36_001681"
     CDS             complement(7073..8116)
                     /locus_tag="ACGE36_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384069.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8117..9058)
                     /gene="opp3b"
                     /locus_tag="ACGE36_001682"
     CDS             complement(8117..9058)
                     /gene="opp3b"
                     /locus_tag="ACGE36_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8384070.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9142..9489
                     /locus_tag="ACGE36_001683"
     CDS             9142..9489
                     /locus_tag="ACGE36_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFF8384071.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9559..11220)
                     /locus_tag="ACGE36_001684"
     CDS             complement(9559..11220)
                     /locus_tag="ACGE36_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8384072.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3195 a         2306 c         2065 g         3791 t
ORIGIN      
        1 aataaaaaag agtaccagat gaatcaatta aaatctgccc gaccctttat cattatgctt
       61 tttttctgtt tttacgattc ctattagtat tattctaaat taacaaaaat cagcgaagct
      121 cgtacttctt tttctaggat ttattttgga tagttctctg tttctgtatt atatgattgg
      181 cttcaagtaa taagaaggaa tttacgaaaa ttgcttctca aatttcggaa aaagggggta
      241 tgacacaact agtgtcatac cccctttgaa tttctatgcg atttctgagc catttggcat
      301 ggattttggt gcttcgatta cttgtaaaga gccatctggt gcttctgcag aaaggatcat
      361 tccttgactg atttgtccac gcatctttcg tggtttcaga tttgctacaa tgacgacttt
      421 tttcccgatc aattcgcttg gatcaggata aaattcagcg attcctgaca agatttgacg
      481 gtcttggctg tctccagcat ctagtcggaa ttgcagcaac ttatctgccc cttctacttt
      541 ttgacagttg atgacttctg ctacttttag ttctactttt tcaaatacat caaatttgat
      601 ttgtttttct ttcgttgaaa caagttctgt ttcttctgga tcccatttga ctgtatcttc
      661 gtttgtttga gtaccttcag acattttttc ttgaataaaa gcaacttctt cttccatatc
      721 taaacgtggg aagattggtg taccttttgc aactactttt gttcctgaag gaaattcgcc
      781 aaagtgtaat ccttcaagat tcattgtttc agagtccaat cctagttgat tgaagatctt
      841 ctctggcgtt tctgtcatga ttggctgaag taatatcgct gcgatacgca aagattcagc
      901 taaatggatc atgacactat tcaattcagc tttcttttct tcgtcttttg ctaataccca
      961 tggttctgtt tcatcaatgt atttgtttgc acgagaaatc agcacccaga tttcagccaa
     1021 tgctgtattg aattccattt tttccattgc atcatggtaa cgtccgacaa cgttagcagc
     1081 cgtcgtagac agttcactat caaatggagt gacaagtgag gcatagtcag gaacgagtcc
     1141 atcacaatat ttgttgatca tagcaatcgt tcgattcaat aagttcccta gatcatttgc
     1201 cagatcataa ttgacacggg aaacaaaatc ttctggcgta aagacaccat ctgacccaaa
     1261 cggaacagca cgaagcaaat aataacggag tgcatctaaa ccataacgtt ccacaagcat
     1321 ttcaggataa acgacatttc cttttgattt agacattttt ccgtctttca tcaatagcca
     1381 gccatgccca aatacttttt taggtagagg caaatcaagg gccatcagca tgattggcca
     1441 atagatcgta tgaaagcgaa cgatttcttt accgaccatc tgaacatctg caggccagta
     1501 tttttcgaat agactttcat cttcggagcc atatcctagt gcagtaatat aattggataa
     1561 tgcatcgatc catacataca ccacatgttt cggatcactc ttgactggta ctccccaagt
     1621 aaaagttgtt cgtgaaactg caagatcctc taatcctggt ttgataaagt tgttgatcat
     1681 ttcgttttta cgagattctg gctggatgaa ttcgggatgt tcttcatagt attccaatag
     1741 acggtctgcg tatttgctca tgcggaagaa gtatgattct tcttttacca attctacttc
     1801 gtgtccactt ggcgctttac caccgatcac gttaccttcg tcatctttgt acacttctgc
     1861 aagttgtgtt tctgtaaaga attcttcgtc agaaacagag taccagcctt catattcacc
     1921 taagtaaata tctccctgtt ctaataaacg atcaaatatt ttttgaacag ctgctttatg
     1981 gtaatcatct gtcgtgcgaa tgaatttatc ataactgata tcaagcgttt tccataattt
     2041 cttgacatca gccgccattt tatctacata ttcttgcggt tttacaccta gttcttctgc
     2101 ttttgtttcg attttttgac catgttcatc cactccagtg agataaaaca catcaaagcc
     2161 catcaaccgt ttataacgag caattgcgtc acatgcaatc gtagtatacg agttgccgat
     2221 atgaagtttg ccgcttggat aatagattgg cgtagtgata taaaaagttt ccttttctgc
     2281 cacggggctc ttcctccttt atcgcatgat gcgaattcgt ttccttgcta gtataccata
     2341 aaaaaagcat gctgttagct ttttttcaaa atgaaaaaga ctggataaac gacttctatc
     2401 tacgttcaat ctatccttta aaaggatgaa taggttgaat aaatgcgggt ttttgttttg
     2461 tttcatatct actaaaaaac ggctttttat ttctcccctc acacgtggta taataaagac
     2521 tatctatctt agtaagggac tggaacaatc atgcagaaga aacatctttt ttttacactt
     2581 agtattgctt ttctttcatt agcacattta atattcagct atttttatat tcgtatgtat
     2641 ggttatttta acctccatgg gcatttgaac agttttatga cagcagcttg gattttgcgt
     2701 tttatcattg acgtatatat cgttatttgt ggcttctttg ccattcgtga agagcggtat
     2761 aaagtattgc cgttttatct gcttttcttt ttattcaatc tcatacttcc atttattttt
     2821 catatttaaa tgataaccta gatttttcaa gtaaatcctt ggtttttttt cagagtctgc
     2881 tacaataagg taaaaagtac ttttactgtc aatggaggtg gtcagatgaa ccgttttgct
     2941 tctaaagaag aagaaaaatt atattatgaa cataaagcaa gtgaagaaga atttcttaac
     3001 tggtatcaaa aacaggaacg tccagaatat gacaagccct ctttgactgt cgatatcgta
     3061 ctgatgtgct ataacaaaga agaagatcag ctgaaagttt tgttgatcca aagaaaaggg
     3121 catccttatc gaaattcatg ggcattgcct ggtggctttg tccaaaagga tgagtcaact
     3181 ggagaaagcg tcttaagaga aacaaaggaa gaaactggtg tggttatttc aaaagaaaat
     3241 attgagcaac tgcatacatt cagtacgcct aatcgagatc cgcgaggctg ggtcgtcaca
     3301 gtcagctact tagcctttat tggagaagaa ccgctaatcg ccggagatga tgccaaggaa
     3361 gtacgctggt tcacactaga acgtcatagt aacaagatcc atttatctag cggagaagtg
     3421 gctatctcgc ttgatttagt cactggagag tcttctggca aagatacttt ggcttttgac
     3481 catagtcaaa tcatcttgaa agcattcaac cgtgtagtca ataaaatgga acacgagccg
     3541 caagttctac aagttttagg taaagatttc acgattacgg aagctcgaaa agtatttgct
     3601 aaattcttag ggatcgatta taaaacgatc gatcattcca attttaagaa agctatgctc
     3661 caatatttcg atgagatcgg tgaacgtcct gtaggaatcg gccgtccttc aaaaatctat
     3721 cagctcaagc cggggcacca tgaataaggt tgtgagcaaa agattcagac ctaatcattc
     3781 aagaaaaaac gcaaaaacaa ctctttgtga gttacgtttt ttgtttcaga attttgatgt
     3841 attcgtaaac tttctgaata aaaaattaac aatagcagaa aaagctcgta cgaacttttt
     3901 ctgctattgt tttattggtt tgtcataatt agtgagcttt tcttcaaaag aaaaattcct
     3961 agaaacaaaa gtcattaaga ctttttatta acaaggctaa ccctaacgct cctatcagtt
     4021 tcattcattt tttatccctt gataaccgaa tgtctgccca ttttttctac tacttggctc
     4081 tttcttacgg aattgctttc ttctgctccg tcggtattga tcaaaagaaa ttttttatta
     4141 taccattggg acttataacg caattttgtt attaatttat gccagtcgtg aatagaaaaa
     4201 tcagcatgtg cctcttcttt tggaaaagat tctacaaata agtagtcata ttgtttgacc
     4261 aatttcccag ataattgttc aagataatct tctcgctggt tcttttgttt cagataaaga
     4321 tccattactt ttctcttttg tttctgataa ttgcttgcct gatctaacct gatctttcta
     4381 tggtgagcgg agcggacttt cacattcaat ttccgctgtg catgttgcag cttttcttct
     4441 gtctggtgaa attttggtaa agttacttct agaggtcgag atgtttggat caatgtcttc
     4501 ggatgatagg cgatcccgat ccatttagct aaagattctt tttttactgg cgaaacatca
     4561 caaagtagtg cgacataaaa ttcttcatta tatcgagcag aaatcgtagc ggagcgaatc
     4621 gatccttcaa ctggccgatg ctgatttatc ttgattagtt ctttttgttt tggtaacttt
     4681 aagtagccat tttccagata gatcgtacgg gtttggttgt tggtcgtata agattgccac
     4741 atactttttt tcgttttaag ctttgggaag ctggcacgtc ccttgaaata attgcgaaaa
     4801 gcccgatcca aattaagctg tgcatttgct aaagcaagac tgtctacttc ttttaagaat
     4861 gggtattctt tttttaatgt agcgggtgtg agcgaaaaat tggctactcc tgtttgttgc
     4921 caacttttct gccgcttttt cagcaaatgg ttataggtaa atcgagcaca tccaaaagta
     4981 cgaatcaatt gctgtttttg ttcttctgtc ggatacatac ggaatttaaa agcttttagc
     5041 atctatgtcc ctcctagtta gcaaccagaa gagattcctt ttgtcgtttt agtttttttg
     5101 cgtaatatgg gacttcctgc tcagttgcat agacaaaatg ttctggtgcg atttcacgaa
     5161 gttgacgtac ttgtccgtca tctggttcag gttgatattt gattcttctg cgttttctct
     5221 catggtctgg atctggtaga ggaatagcag ataacaaact ttctgtataa ggatgaacac
     5281 cgtgattata gatttcatca cttgttccca tctctaataa caagccgtta tgcattacac
     5341 cgattcggtc actaatatgt ttaaccatcg acaaatcatg tgcaataaat agataggtca
     5401 attgatgttc tttttggaga tcttgcaaca aattgaccac ttgggcttgg atcgacacat
     5461 ctagtgcaga aattggttca tcacaaatga taaaacgcgg ttttactgcc aatgctcgtg
     5521 caataccaat acgctggcgt tgcccacctg aaaattcatg aggataacga gtaccgtggc
     5581 ttggattcaa tccaacagtt ctcaatagat catcgacttt ttttgcacgc tcttcagatg
     5641 atttagctag tccattaaca tcaattcctt cggcaatgat atctctgact ttcatacgag
     5701 gattcaacga agcgtatgga tcttgaaaga tcatctgaac atcatgccgg aatttcgtca
     5761 aacctttttt atctttgatc ttcgttatat cttgaccgtc gaataatatc tcaccatcag
     5821 taggttcatt caagcgaatg atcgtacggc cagtagtgga ttttccgctt ccagattctc
     5881 caactaaacc gaatgtttct ccttcataaa tgtgaaagct gatgtcattt acagcatgta
     5941 cttcattctt ttttcctaca ttgaaatact gtttcaatcc tttaacttca agtagtttat
     6001 ttggcatcag caattcctcc ttgaccgtaa gtatgttttt gtttctcttt aaagatagcc
     6061 cagcgacgtt ggatttcttc tggcggtgtc actttgggtg cctgcggagc aagtagccaa
     6121 gttgctgcct tgtgtgtttt tgatagttca aaaaatggtg gttcttgttc tgcatcgatt
     6181 ttcatcgcga attcatttct tggatagaat gcgtctcctt ttggcgggtt caataaatcc
     6241 ggtggagaac caggaatagc atacaatcga tcgttcgcac tatctaatgt cggcattgaa
     6301 ccaagcagtc cccaagtata gggatgttgc gggttgtaga aaatttcttc cgatgttcca
     6361 acttcaacga ttcttcctcc atacatgaca gcgacacgat cagcgacatt cgctactaca
     6421 ccaaggtcat gagtgataaa gataattgat gtatcgattt ttgtctgaat ttctttcagc
     6481 agctccaaaa tctgtgcttg aatagtcaca tcaagcgctg ttgtaggttc atcagcaatc
     6541 aggatttccg gataacatac cagtgcaatc gcgatcacga tccgttgacg ctgacctcct
     6601 gagaactgat gcggataact gttgatacgt ttctcagctt cgggtattcc aactaattta
     6661 agcaattcta atgcagcttt ttgtccctct ttttttgaaa ctttattatg ctttctaagg
     6721 gactcagcga cttgcttccc aatgggcatc gtcggatcta aagaggtcat tggatcttgg
     6781 aaaatcatcg ctatttcttt accacggatt ttttgcatct ctctttctga ttttccaacg
     6841 atatcgttac ctttaaagag gatttgacca gcatctatat tggcgttgct tgacagcaag
     6901 cgcatgatgc ttctggtcgt cactgatttt ccacttccag attccccaac gatcgccaat
     6961 gtttctcctt tattcaaatg aaaattgaca ccgcgaatcg ctcgtacctt tccagcatac
     7021 gtatcaaaag agatttctaa gtctttcact tctaaaattt tcgtcatcgt cttcactctt
     7081 tcattttagg atcaaaggca tcacgcaatc catcagcaag taagttaaag ccaatcatga
     7141 taaccgataa agttactgcc ggaatccata atagataagg cagatacagg aatgtcttgt
     7201 acccttcgct caacatcgtt cctaatgaag ctgacggagg cgtaagacct aaaccgataa
     7261 agcttaaaaa ggcttcgaag aaaatagcag atggaatact aaacatcatt tgaataataa
     7321 tgacactaga gatattcggc aaaatatgtt tgaatgcaat tttcattctt gattcaccta
     7381 atgtcaatcc agctaaaaca tattcttgat cttttaattt cattgtttgt gcacgaacga
     7441 tacgtgccat tggtatccag tttgtgatgg ccatagcagc cacgattgaa aaaattcctg
     7501 gttcaaaaac gaccaacatc aaaatcatga caacgaggtt tggaattcct gataagattt
     7561 ccaaaaaacg ttgcatcacg ttatcgacac gtccgcctaa caaaccagaa accaacccgt
     7621 aagctacacc aatcgttaca tctagtaatg cagcaataaa tgcgatcagc aacgagattc
     7681 tcattcccat aaataagcgg cttaatacat cacgtcccaa accgtctgtt cctaaaaaga
     7741 aatttacgtt tgaagggacg tttgcttgag catatttatc aacaagttca ccaccaacca
     7801 ttgcttttcc attcaatcca tcgatattga ttcccggaat acgaggaggc aaattgatat
     7861 aagccacgtt ttgggcagtt ggatcatgag gagaaacaaa gattgtgatc acagagatca
     7921 aaatgatgac caatagtaaa accattgaaa taacggcagc tttgtttttc tttaaacggc
     7981 gccatgaatc ttggataaaa ttcaaagaag gtgcagtaat ctgttcacgt tcttcttcgg
     8041 tatttttttg taatggaaga aattcatccg cagggatatc tgcaactgtt tcataacgct
     8101 ttggtatcat ttccatttat cctcggctcc cttctgaaac acgaattctt ggatcaacta
     8161 gaccgtacaa tagatccacg atcaaaataa caaaaatcag cataaatgag taaaggatcg
     8221 ttacagccat gatcgtagga taatcatttg ttgtgattga cttaacgaat tgttccccga
     8281 taccaggaat agcaaagata ttttctacca ccagtgatcc ggtcatcaat gctacagcta
     8341 acggtcctaa taaagtcatc aatggaatca aactatttcg aagaccatgt ttaaaggcga
     8401 tttcccaacg gctcaatcct tttgcttttg ctaattccac ataatcacta tgcagtactt
     8461 ccaccatttc cgtacggata aaccgagcag agtcagcaag cggcgacatg gccaacgcaa
     8521 ttgttggtaa gattgtgtac acaaagccat cccattttgc gattggcaaa acttgtaatt
     8581 tgattgcaaa gatatattgt aataaaacag caaatacaaa gtttggaatg gaacgaccta
     8641 atatcgctac gagtgtagaa gatgtatctg cccatgtatt ttgcttcatg gcagagatcg
     8701 ttcctaaaat cgtccctagg actgttccaa agataattgc ctgtaaccct aactgcaagg
     8761 atggtccgat tcttccagct aacaaatgtg cgactggctg gtttttaaat tggaaggaaa
     8821 ttccaaaatc tccttgaagc aaattagaaa gataaatccc atattgtacg ataactggtt
     8881 tatccaaacc gacttgttta tttagcatct cgattgtttc aggactcaaa cgttcctgat
     8941 tcgtatatgg cgtgcccggc agcaattgca tcaagaaaaa ggtgattgtt gcaattaacc
     9001 aaagcgtgat gatcatgaaa aagacacgtt ttaatacata tttgatataa ctattcacta
     9061 atgtttcccc ctagcttttg acttctgaca gatgtttcgc tacattatta gataatgaac
     9121 caaaaataaa ggagcaatca aatgaaaaaa aaatctatcc cctacgcagt agcttttcta
     9181 ctgattctgg ttattttaat aaaaaatctc atcaaccatt cctttacact cattcaatta
     9241 tctaatgatc tattcttatg gtcattgccg tttctgatta tcggcggttt tttatgggtc
     9301 ttttcttcgg gtttcttcga tcattttcag cgatcggtcc atcttgcacg aacaaggaac
     9361 cgaaagaaaa aaccagaatt ttcctcgctc tcgtctgcca gctacggtat gtacagcttt
     9421 tggctgatca ttgccggaat actaattgct ctttctgcta ttttcatgct tttttctttg
     9481 ttaggataag tgaatatgag aacgaggttg cgacatttgc cgcagcctcg ttttttctca
     9541 tgctcacggt attaacgatt attcttcaac gtatgtccat ttgtaatccc atgaagcgcc
     9601 tgccgcatgg tgaacgatac ctttgatttt ttcattcact aggtgacctt ctgctttttg
     9661 gtacaccgga acgacaccca tatcatcaga gatgatcttg attgcttttt ggaaatcatc
     9721 ccaacgggct tgttcatcgc cagcgtctgt tgatgaagct gctttaactg cttcgtcata
     9781 ttctgcattg ctccagcgac cacggttgta tgaattacct gtgacaaaca aatcagtaaa
     9841 gctgcttggg tctgcatagt cagcagccca tccgcctagt actgcgtcaa aatcacccga
     9901 agttgagcgg tcaagacgta cagagaatgg aacaggtgtt ggtttgactg ttacgccatc
     9961 caagttttct tggattgagt tttgaacata ttcgattact tttttcgatg aatcatcatc
    10021 agaagccatc aaatcaaatt ctaaagaatc aacgcccaat tcttttttcg ctttttccca
    10081 atattctttt gctttatctt tgtcataaga aactaatttt ccagcttctt ttgcaaagtc
    10141 ttcgttcgtt gttggacttt ttaccatatc tgatgggatc aatcctgtag agacaactga
    10201 tccgtcacct agtacttgtt ttactaaagc ttcacgatcg atagaataag aaatagcttt
    10261 tcttaggttc acattgttga atggtgaatt cttttcacgt tgattgaatt cgatatagct
    10321 tgtccgcgct tctttaacag aagtataagc tggatcgttt gcgttttgct gtgctaactc
    10381 accagacaag atcacgtcat ctgcttggcc atctttgaat aagttcaatg cagtagaaga
    10441 ttcttttact acactgactt tgatttcaga aagttttact gtatctttgt cccagtaatg
    10501 atcatttttc ttataagtcc attctgtatc tgttcctggt ccatcaaaat cttctaatgt
    10561 gaatggtcca ttataaacag ctttatcact tgcagatgca taggcatccc cattttcttc
    10621 cactacggat tgtttttgtg ggaaaaatga tgggaaagca agcagatatt ggaaatatgg
    10681 tgtttgtttc gtcaatttga tttctaattc atagtcacca actgctttga tccctagttc
    10741 tgatttatct ttctttccag ctgtaatgtc ttctgcattt tcaactggcg catacaggta
    10801 agcatattca gaagctgtag ctgggtctac tgttcgttgc cagccataaa cgtaatccgc
    10861 tgctgtaaca ggatcaccat ttgaccattt tgcgtcttct cgtaatttga ttttgtaagt
    10921 taatccgtct tcactgactt ctgccaactc gctagctcct gccggctgtg gtttgctgtc
    10981 ttcatcaaga cggtaaattc cttcataaac gttattcaat gctgtaaaac tgattgtatc
    11041 tgttgcaact gataagtcag ctgtaggcat ttcttgaggc acgactaagt taaatacgcc
    11101 tcctccatct gctgaaccgc tttttgctga actactgctt gtggctgaac tgccgccata
    11161 acatccagca agtacaaggc cgcataccgc aaccaaacca aatgtaaatt tctttttcat
    11221 tttacgtacc cctttattgg aattttcaga ataattaata ttttctgaag ttgtttaaga
    11281 gtttaatgaa taattattaa aaaatcaaca cttttttttg tctttttcat aaaataatga
    11341 ttttttcgtg aggtttc
//
LOCUS       DAXWLH010000081        11167 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXWLH010000081 DAXWLH010000000
VERSION     DAXWLH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.081"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(197..1045)
                     /locus_tag="ACGE36_001685"
     CDS             complement(197..1045)
                     /locus_tag="ACGE36_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8384073.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1209..2111
                     /gene="gnd"
                     /locus_tag="ACGE36_001686"
     CDS             1209..2111
                     /gene="gnd"
                     /locus_tag="ACGE36_001686"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFF8384074.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2113..3114
                     /locus_tag="ACGE36_001687"
     CDS             2113..3114
                     /locus_tag="ACGE36_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFF8384075.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3111..3395
                     /locus_tag="ACGE36_001688"
     CDS             3111..3395
                     /locus_tag="ACGE36_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFF8384076.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3428..4225
                     /locus_tag="ACGE36_001689"
     CDS             3428..4225
                     /locus_tag="ACGE36_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8384077.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4239..5072
                     /locus_tag="ACGE36_001690"
     CDS             4239..5072
                     /locus_tag="ACGE36_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8384078.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5090..5947
                     /locus_tag="ACGE36_001691"
     CDS             5090..5947
                     /locus_tag="ACGE36_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8384079.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5969..6478
                     /locus_tag="ACGE36_001692"
     CDS             5969..6478
                     /locus_tag="ACGE36_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8384080.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6515..6916
                     /locus_tag="ACGE36_001693"
     CDS             6515..6916
                     /locus_tag="ACGE36_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384081.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7028..7381
                     /locus_tag="ACGE36_001694"
     CDS             7028..7381
                     /locus_tag="ACGE36_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384082.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7415..7858
                     /locus_tag="ACGE36_001695"
     CDS             7415..7858
                     /locus_tag="ACGE36_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384083.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7875..8960
                     /gene="uxuA"
                     /locus_tag="ACGE36_001696"
     CDS             7875..8960
                     /gene="uxuA"
                     /locus_tag="ACGE36_001696"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8384084.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8953..9666
                     /locus_tag="ACGE36_001697"
     CDS             8953..9666
                     /locus_tag="ACGE36_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8384085.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9873..10022)
                     /locus_tag="ACGE36_001698"
     CDS             complement(9873..10022)
                     /locus_tag="ACGE36_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384086.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10418..10618
                     /locus_tag="ACGE36_001699"
     CDS             10418..10618
                     /locus_tag="ACGE36_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8384087.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3736 a         1750 c         2411 g         3270 t
ORIGIN      
        1 actaactttg ttatcgcatc taacaagtaa ctcgaataat accaatacaa ccttctccaa
       61 agatgcgatg acatacgttc taacccaccg actattgatg ttctgtacca cccttgcaga
      121 accaaaaaag ccactaccaa atatttggta gtggcttttt ttgttttatg gatttttcgc
      181 tttttctgtt atggtgctat tctttgattt cagaaatagt atgtcgaact ttagcgatag
      241 aacgacgtga ttctttttcg ttgtaaaaca tcaaaatcac atatagagag tccaaaatgc
      301 ttagttgaga aatacgggaa gctaaagctt cagatcggaa ttctgtttct tcagaaatag
      361 aaataaaaac gacatcgcca agtttagcaa gggaagaatt agcttggcta gtaataacga
      421 ttgtttttgc tccagttttt cttacggttt cagcaatatg gattgtttct tttgattgcc
      481 ctgtgtgaga aatcaaaaca gcacagtctt tttccgtcaa taaagaagct tccatcaatt
      541 gaatatggta atccgtgctg tgagaagttg cgatagggga acgcaaaaat ttatgataag
      601 cgtcagttgc tacaatctct gaaccgccaa caccgaaaaa atagacacgt ttagattcat
      661 tgataatagt agccgctttt tttagatcct tttgttgtaa caactttttt gtgtcattta
      721 aagttgaaat actagactcg aacacctttt gcgccatagt gagctcagta tctgttttct
      781 gaatattttc gtggatagaa atagcagata gatcattttc ttggatcaac atggccattt
      841 taaaatcttt aaatccatta taaccaagtt tttttgtaaa ttgaaaaaaa gtagagtcag
      901 caatgcccag ttcacttgct aaattgctaa taggactatg acttactttg cttggatttt
      961 ctaaaatata atcggcgata gcctgttctt ttgggctgaa gtcgttgtac attgttttga
     1021 tttgtagtct tatggagacg ctcatgagaa ttcctcattt tcataataag attatcattt
     1081 tttccgttct cattaatgtt aaagatacaa ggagagtagg aaaaaatcaa acaaaaaata
     1141 tttttgtaaa atctatttac aaaaaaattt ttttgtataa tgagggtata agaaatggag
     1201 gcgctatcat ggaaattggt gtaattggtt tgggaaaaat gggattgaat ttagctttaa
     1261 acttaaaaga gcaccatcac aaagtacaag gactcgacat ttcaagtaca gctgttagtc
     1321 aggcaagaga aaatgacatt cctgcttatc aagagatctc tgaatttcta gctacttttg
     1381 agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga gataaccgaa aacatgttaa
     1441 gaaaactttt tccaaaactc ggaccaggag acattattat tgaaggcgga aactcttatt
     1501 ataaagattc aatccgtcga gcagaagagt ttcagaaaaa tgaaatcggt tacttggatt
     1561 gtgggacttc tggaggaatc gaaggagcac gacatcaagc ctgcttaatg attggaggag
     1621 ataaagaaac ttttctatcc gtggaacaat tattcaaaga tgtagcaatt gaaagaggat
     1681 atctgtatgc tggtgcatcc ggaagcggac acttcttgaa aatgatccat aatggaatcg
     1741 aatatggaat gatgcaagca atcggtgaag gattccaact tgtacaaaaa agtgagtatg
     1801 cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg ttcggtgatt cgcggctggc
     1861 tgatggaaat catggaagat cagttaaacg aacatcccaa tttaagtaat tatcggggaa
     1921 tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac agcgctagaa atggctgttc
     1981 cagttccagt aattgcaact agtttgttca tgaggaatct ttcacaggaa gaagatagtt
     2041 tttcagcaaa agtagtttca gcactaagaa atgggtttgg cggacatgaa atagttgaaa
     2101 agggtgagta aaatgccaga ttatcatata gcaatcgtta attccagcag tttcggaaga
     2161 gtctttccac accacttgga aagattaaag tcagttggag acgttcattt ctttactgtt
     2221 gatcaagaga ttccaggaaa agaactagcg gagttactta aaggattcaa tatcattatt
     2281 gcaagtgtta cgccattttt tcgacgagat ttttttgaaa acaaagatga actgctattg
     2341 atttcccgac atggtattgg ttacaacaat attgatttaa aagcagcaaa agaacaccag
     2401 acaatcgtat ctatcatacc tgctttaatt gaacgaaatg ctgtagcaga aaataatata
     2461 actaatttgt tgtccatgct tcgacgcacg acacaagcac aagcaagagt caaggacgat
     2521 cgatgggaag atcgggcgga gttcgtcggt aggacgctgt ttaacaaaac agttggtgtg
     2581 attggtgcag gaaataccgg aagcggagta atcgaaattt tgcgtaacgg ttttcgctgt
     2641 gatgttctag cttatgatcc ttacaaatca gatttataca tcgaaagctt tggtgcaaaa
     2701 aaagtagaac ttcaagaatt actggaacaa tcagatatca tttgtttatg tgcgaatttg
     2761 acagaagaga actatcacat gatttctgat aaagaagtcg atcagatgaa agaaaatgtt
     2821 tatctatcta atagtgcaag aggcgctcta gttgatgaag aagcgatgat taatggtctg
     2881 aaaagcggaa agatcgcagg atatgccaca gatgttttag aagaagaacc gggacgtcaa
     2941 gatcatccgt atttagcgtt tgaacaagta gtgattacgc cacatatatc ggcctacaca
     3001 agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag atgtcgaaca gatcagtcag
     3061 caccttcttc ctgaacgttc tgtccagaca aagagcaact atataaaagg atgatgaaga
     3121 aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt ttatttatat gaagaaggtg
     3181 gtatttcaaa agtttctgat ctcttgaaat catacggcgc gaaaagagta ctggtcaccc
     3241 acggaacggt atcatgggaa aaggcgctac ctaaattagt atttttgaat gacgaaacga
     3301 ttcaattttt ttatcatcgt tattctggag aatgtagtta tgctgaagcc agacgaatag
     3361 ctacaatcat caaaaaaatg aaatcgattt cgtagttggc gttggcggag gaaaactgac
     3421 cgatctagtg ggttatagtg ccaatcttgc aagaatcgat ttcggagtca tccctacttt
     3481 agctagtaat tgtgctccat ggacacctct tgctgtcatg tataaggaga atggacaagc
     3541 agaagggaaa acagaacact tttacagaca agctgctttt tttataacag atccccaatt
     3601 agtgattgat tcccctgttc catatttcat tgccgggtta gcagatacac tagcaaaatg
     3661 gtatgaatct gagctcatct tagaacaaga ttttttgcaa gatgaatcat ttttgaagct
     3721 agcacaagat actgcgaaga tctgcaaaga agagattttg aatcattctg ctaaagcaat
     3781 ccaagatatg gcacaaagaa aactgacatc ggagttcaaa cagctttcag agattatctt
     3841 tgctgttgct ggtttagttg gaggattagg agataaatac gcaagaaatg cggcagcaca
     3901 tgctatgcat gacgctatca gtaaatatat tccggaaagc catcgttttc tacatggaga
     3961 aaaagtagcg tatgggattt tctatcaact ggcactagaa ggtcgctggg aaaaaataga
     4021 cgagttatat cctttgtatc gggaattaga actgccggtt tctctagaac aaatgggatt
     4081 atttccgaaa gataaacaga taatagataa aatggctgaa tttattgatt cgaaagagaa
     4141 agtgcatttg attcctatag aggtaacaca gaaatcactg atacagtcaa tgatggaact
     4201 ggaaggttat atcagaaaga tctgaaggag gaggacacat ggaaacatta agtgtagtac
     4261 aaagtttgtt gattgcttta tgggtagccg cggttatgtc ccgctggtta ggtggaggag
     4321 ccacgttgac cttacgtttt tctcctttga tgactggatt agtcgtcggg ttgatcatgg
     4381 gggaagttgc acaagcgatg attgttacag ccgctttgca aatgatttat atgggggttt
     4441 tttctccagg aggatcaatg ccagctgaac catctattgc agcagctatc gcagtaccag
     4501 tcgcattatt aggggatctg tcgccagaag cagcgattgc cgttgcggtg ccagtcggat
     4561 tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa tactttttta ggtaaatata
     4621 cagacaaagc tgtagaagat ttgaatacaa aagctatggc ccgatcaatt attctttatc
     4681 caacggttgc ctcattttta ttattcgttc cgcttgtttt cattgcttta tatttagggg
     4741 cgccattgat cgctgatgta atcaaagctt tagagggaac agtcgttatc cacattcttg
     4801 aagtagtagg tggagggtta gcggcaatcg gtatcgcgac aactgtatac gttatcggtc
     4861 gaaaagatta tctggtcttt ttcttccttg cttattttat gagtgttgtt ttgaaatctt
     4921 tagatattac gatggttact tatgccattt ttgggattat tatcgctttg atctttgtcc
     4981 agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa taacaattca gcatcttttg
     5041 atgacgaaga tgactatgat gacggatttt aatttaggag gaatgggaaa tggctgaaat
     5101 aatggataat caaacaggtg ctcctgaaga aatcacgaaa aaagatgtaa caaaagccta
     5161 tctgagatgg cattttgcaa acgaaattcc tcactctttt gaacgttact tggcgccatc
     5221 actactatac ggaatgatgc caatcttgaa aaaactttat aaagatgaag actctttaaa
     5281 agcagcatat aaacggcagt tactgttctt caacactcaa ttaagctggg gtggcggtgt
     5341 gattactggc ctaatgtctt ctatggaaca gcaaagagca gaagaagagt atgaagaaaa
     5401 agagatcttg atgcaggatg acttgatgta taacacgaaa gcgggattga tgggggcact
     5461 agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa tatattttta tcgcaatcgc
     5521 ggtaccttgg gcacaacagg gaagtccttt aggcgcactt tttcctttta tctgctttgg
     5581 gttgtatcag gttttactag gggtattttt cgcaagacaa gctttcacta tgggccgtaa
     5641 tgctacaggc ctaatgcaaa acactggtgt tcagacagcg attgaaatgc tatcagtttt
     5701 agggttgttt atgatggggg tgcttgcggg aaactatgta aaagtagaat cttcattgaa
     5761 atttgcgata tccggacgtg agtttgttat ccaggaaatt cttgatcaaa tcgtgccagg
     5821 gatattacct ttagctgtcg taatgggtgt gtacttgttc tatacgaaaa aaggattgaa
     5881 agtcacacaa gcattattat ggctgactgg aatattaatt gtgctagcag gaatcggtat
     5941 tttataaaaa gaagggaacg tgaataagat gggtgtagtc aatttagcgc gtgtggatga
     6001 gcgtttgatc catgggcaag taatggtaac gttatctcaa aaaagtggag tgaattccat
     6061 ttttgttgta gacgaagttg tagcaaaaga taaatttatg agagatcttt acaaaaatgc
     6121 aggaagccgt acaggacaaa aaacaattgt cattacacca gagaaagcta aattctattg
     6181 ggatgaatat caatttaaag aatacaattg tattttgatc gcaaaaacag tccaaacgat
     6241 ttacgactta gtaaaacacg gaatccccat ggaagaattg aatattggag ggattgctca
     6301 aaaaaatcct gatacagatc ttttagtgac gaaatctgtt tatttaaata aatcagatgc
     6361 tgaaaaattg aaagaaatga atgaacaata tggcgtaaag aatatttatt ttcaagccac
     6421 tccatctgca cctaaaacag gattaaaaga tgtattgaac aagtttgatt tgtcataaac
     6481 aaatgcttag aaaaaagata aagagggcga aaaaatgaag aagaacgagc tgtttcgtga
     6541 ttgggaattt cgttatcgct atatttaccg taagcgccgt acaaaaaaga gtaaacagcg
     6601 ttttctttca gcattagtat cagatattta cagtatgaga acagatgtga cagtcattgc
     6661 ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt ggagatatcg aaaaagcaga
     6721 gaaagttatc tgtacttatt acgatacacc cgttcatgca ttaggaagtt atttcatgtt
     6781 tgattggaaa gaccaaagga aaaaaacaat ctactctata ttattgtcct tcattttgtt
     6841 gttcagttta ggttggtggg gaatgatgat atataacaaa aatcctcatc acgtattcga
     6901 tctgctatcc gtataaacag gtatcactgt actagcattt gggagctact ttttcttatt
     6961 gggaaaagca gctcgcggat ggcctagcag acaaactttt attcgaaata cttcatccat
     7021 attaacgatg ctagaaatga tccgtacgat tgatgatccg agtgtagcgt atgcgtttgt
     7081 tgatgaaggc tgttatggag aaaagggctt agacagcgtg agatcgggta tgaaaaaaga
     7141 ggctattctt ttttatctag actctgttgg ggcagataca ccgctacagt tttcgggaaa
     7201 ttatttttca aataaagaac agtggctgaa acaagttgat aagttgaaag aaaaaaacgt
     7261 caattatatt tttagtgcaa gaaaaaagca agcgcagttc ttctatttga ccaaaacaga
     7321 tttacgaggg aaaacattta attggcaaaa tgccaatcag attattgctc tttttcgata
     7381 agtaaaaagt gcaaaacaag gagtgagaag actaatgttg gaaatcatga tcgttacaca
     7441 tggtaagttg agcgaaggac tgaaagatgc ggcggaagta attgtcggga catcgagcca
     7501 tattgaaaca atgtgcttag agtctgggga ggacatccaa acattcggag agaaggtcaa
     7561 agaaaaatta actatgatca acacagcaga acccatgcta gtatttgttg atctaattag
     7621 cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa ctgactgaag aaaagaaagc
     7681 agccacttat gtcatagggg gcgtgaatct gccaatgttg ttagaagcaa tcaatcatca
     7741 gatgcttcac acaccgatag aagaagcggt ggaacaaatt gtcaaacaag gaaaagataa
     7801 tttaactgtt tggcatgctt cagcggaaga aaaaaaatta gatgaggacg atttttagaa
     7861 ggaaggctga aaagatgaag tggggattta gatggtatgg tgaaaaaggg gattcgatcc
     7921 cattaaaata tgttcgccaa ataccgggaa taaatgggat aattggaact ttgctgacta
     7981 aaatgcctgg agatgtttgg gagatttcag aaattgagga gttgaaagca tctgttgaga
     8041 gccaaggact ggaactgctt ggaattgaaa gtgtagcgat ccacgatgca atcaaggcag
     8101 ggacagatga acgagattac tatattgata actatatacg aacaatcaaa aacttgtctc
     8161 gatgtggggt tcgattgatt tgttatagct tcaaaccaat ttttggatgg gcgaaaactg
     8221 atttgtttaa tgaaaacaac gatggaagct attctctttt atacgatcag aaaatcgtcg
     8281 atcagatgga gccaggagaa atgtatcaat tgatacacag ccagtcaaag ggattcaaat
     8341 tgccgggttg ggaagaagaa cggttgaaaa aattcgctca gctggaaaaa ctgtatgaag
     8401 gaatgaccga agaagaatta tttgaaaatc tgacttattt tttagaaaaa gtgattccag
     8461 tgtgcgaaaa agcagatgta aaaatggcta tccatccaga tgatccgcca tgggaacttt
     8521 ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa gaaaattcta aatgcagtag
     8581 attctccata taatggacta acgctgtgca caggatcttt aggggcaaat ccggaaaacg
     8641 atttagttga aatcatccat gaggtaggtt cgcgcatcca ttttgtccac ttcagaaacg
     8701 tggagttttt aggagaaaga aagttcaaag aaacggcaca tccctctaca gaaggctccc
     8761 ttgatatgta tcatattatg aaagcgttgg tgcaagaagg gttcgacggt cctatacgcc
     8821 ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc tggatatggc ctgtatgatc
     8881 gtgcaatggg actcacttac atgcaaggtc tatatgaggc gataagtaag gaaaaaccac
     8941 tgggagagca ttatgaataa aatcaagctc ttcatctttg atatggatgg ccttttatta
     9001 gaaactggac gtttagctta tcgtgcttat gtaaaatcag cacaaaagta tgattatgaa
     9061 atgcgtaagg aagtctatta tttactaact ggccaaacag aaatggcgat aagagaacaa
     9121 atgggcattt tgtacggaga agatgttcca tatatcaaat ggcgagaagc aatcaatcaa
     9181 tataaagaaa aaatcgtgaa agaagataag agagtatata cgaaaaaagg agcagaagaa
     9241 attttatctt ttgcaaaaga aagaggaatc catacaatcg tggcttcgtc taatacacgg
     9301 gtaaaagtag aaatgtattt aaaaatggaa aatctatatg ccttgtttga tcaaatcatt
     9361 tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga tctttttgaa agcttgttca
     9421 aaaatgaata ttcctccaag tgaagcactt gtatttgaag actcgattgc agggatcgaa
     9481 gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag atcatttgat agggttgccc
     9541 gatcataatg ggaagcataa gttgaaagta gatgtaagtt tatatgaatc caataaagca
     9601 gactatgaat tttatgacct gcatcaagca aaacgatttt tagaaagtaa agggttgtat
     9661 ctctaataaa gaggactatt ttctagtcct ctttatttat tgtcctgcac gactgaggga
     9721 acagatgaaa aagaagtaat tttttatttt gaagtatcta ccttatttgt agtgaacaat
     9781 ttagaacaaa cacaaaacgc atctactgtt atctaccagt agatgcgttt aaagagaaga
     9841 acgagaatcg tccatatgag ggtttccact ccttatgcag atttttttaa ttcttgcact
     9901 gcttgttcga tcgtaacacc tgtcgctaca tgattggtat tttctttgtc aaaaacgaca
     9961 aatagattca aatctgtatc caaggttact cgatattgta tatctttgtt atagaatgtc
    10021 atagccattc tcctttcttt aaatacttgc tgaaaggcaa cgaacgcgtt gataacgaaa
    10081 ttccatttat aagtatacca ccaattgaaa ggttttcata gttaagaaat tttaaagtca
    10141 aaagttgcag ataaaagcaa ataaacaaga aaacaaaaag tgaatcaatc gcggttattc
    10201 aactttttct tcgattcgaa gacaatagaa gacaatgaat tttatatttc attgcattat
    10261 ccttgtgtta ttagaaaaag catgatatat taaatgagta atctattttg aaacgtaatt
    10321 tgagcgatat atctacatag ataaaactcc ttttgcctat caaatattta ttgcaacgaa
    10381 aaaacgtaca ttgatacgta tcaggaggaa tttatttatg aataacggta cagtaaaatg
    10441 gttcaacgca gacaaaggtt ttggctttat cactggtgaa gatggaaatg atgtatttgc
    10501 acatttttca gctatccaag gtgacggctt caaaacttta gaagaaggcc aagcagtgtc
    10561 ttacgatgtt gaagatggtc aacgtggccc tcaagcaaca aacattgtta aagcttaata
    10621 aaaattgact tttaaccgtc ttttcgaaag aaaagacggt ttttttgtac gaaaaaattc
    10681 attggtcgtt gaatgggctg aatagtagtg tataaaacaa ttttgtggag cgtgaagctt
    10741 ttatggtaga ggaaaatacg aataaagtga tgttgataaa aagattccta tcttgaatat
    10801 gaatcgagat gtcttgaaaa tgaatgagtt tgaccaatca ttatcctatc gaaatttaca
    10861 gtttcgttcg attggtctgt caattaatga aaaagtaaaa aagaatatgt tctaggaaga
    10921 gggcggcaca tctaaatttt taagtaacgg acagttcaaa tatcaagcat tgaaagaggc
    10981 tatcctactg tttgaaggaa tacgaatata taaaaacgac aaagaggaaa aagtagttca
    11041 ttaaatccct tcaagtgatg aaagaatgag atctaataaa acctgatctt tcatctgttt
    11101 ttttaataac gtaataattt ttttattttt tgtgctttcg ccgttattgt agagtgattt
    11161 agcattc
//
LOCUS       DAXWLH010000082        11049 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXWLH010000082 DAXWLH010000000
VERSION     DAXWLH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.082"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..1435)
                     /locus_tag="ACGE36_001700"
     CDS             complement(<1..1435)
                     /locus_tag="ACGE36_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384088.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDK"
     gene            1557..2141
                     /locus_tag="ACGE36_001701"
     CDS             1557..2141
                     /locus_tag="ACGE36_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384089.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2152..2397
                     /locus_tag="ACGE36_001702"
     CDS             2152..2397
                     /locus_tag="ACGE36_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384090.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2403..3125
                     /locus_tag="ACGE36_001703"
     CDS             2403..3125
                     /locus_tag="ACGE36_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384091.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3256..3999
                     /locus_tag="ACGE36_001704"
     CDS             3256..3999
                     /locus_tag="ACGE36_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384092.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4005..4370
                     /locus_tag="ACGE36_001705"
     CDS             4005..4370
                     /locus_tag="ACGE36_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384093.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4386..4916
                     /locus_tag="ACGE36_001706"
     CDS             4386..4916
                     /locus_tag="ACGE36_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384094.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5078..5386
                     /locus_tag="ACGE36_001707"
     CDS             5078..5386
                     /locus_tag="ACGE36_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384095.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5517..5801
                     /locus_tag="ACGE36_001708"
     CDS             5517..5801
                     /locus_tag="ACGE36_001708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384096.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            5938..6738
                     /locus_tag="ACGE36_001709"
     CDS             5938..6738
                     /locus_tag="ACGE36_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384097.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6775..6957
                     /locus_tag="ACGE36_001710"
     CDS             6775..6957
                     /locus_tag="ACGE36_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384098.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7068..7385
                     /locus_tag="ACGE36_001711"
     CDS             7068..7385
                     /locus_tag="ACGE36_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384099.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7388..7531
                     /locus_tag="ACGE36_001712"
     CDS             7388..7531
                     /locus_tag="ACGE36_001712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384100.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7735..8214)
                     /locus_tag="ACGE36_001713"
     CDS             complement(7735..8214)
                     /locus_tag="ACGE36_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384101.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8298..9671
                     /locus_tag="ACGE36_001714"
     CDS             8298..9671
                     /locus_tag="ACGE36_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFF8384102.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9694..10221
                     /locus_tag="ACGE36_001715"
     CDS             9694..10221
                     /locus_tag="ACGE36_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384103.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10208..10771
                     /locus_tag="ACGE36_001716"
     CDS             10208..10771
                     /locus_tag="ACGE36_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384104.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
BASE COUNT         4070 a         1438 c         2119 g         3422 t
ORIGIN      
        1 ctttatcctt taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac
       61 tataatgttt acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa
      121 taaaaggcgg aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta
      181 atttgttaaa ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct
      241 tatctgctaa cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca
      301 tctctttttc accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta
      361 gcgaagaaac tctcattttt tgctggagta taggatcaac tagactattt aagtgatttt
      421 gtaattcttc attgttgttt cctagaactg caagggaacg taaacggtag taatattcta
      481 tttctgttag tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa
      541 ttgtctcgtc tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa
      601 agaacccttt tgtatacgtg atctgttgat tttcaccaag tgatttatta atttcctctc
      661 ttgattcagc tgtgaaccta tactttgcaa tttctttttt tagaatattt aaatagaatt
      721 ctttattatc aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg
      781 cttcctcatt tccataagat aattgaaaaa caaacttaaa ggctgtctta cataggtttt
      841 ctttggcttt ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt
      901 caggtgtaac agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt
      961 tttttcctaa tattggattt tttaggaata taatttcata ggacttccgt ataacatcct
     1021 gacaagtttc ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag
     1081 gatgagctga ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat
     1141 caattagctc agttgaacta taagcttttt ctagtagttc catttcccat tttggagagt
     1201 ttggatcact ttttcttagt ctagctatat cttctaaaat tttcttagaa gtattatcct
     1261 cgtaaatatt tgctaaatca gataatttat caattaaatc taaaataagc gcactgtaat
     1321 tgacagctat tgcagaacga taatttccct gataaaaact acttctaact tcttctaata
     1381 atggaataat tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa
     1441 tctccttttt ttatttgtat gacaagttta ccataaaaaa aaattaaaaa gcgagacata
     1501 tttatgttgt tgtgttatca taataacata aatatgtaga gtgagatgat gaaaaaatgg
     1561 atttgaaaca atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg
     1621 atatttttgt agttagcatc ttattgtatt tattttatct aattttcttt cacaatttaa
     1681 tcgggtacat gattcttgat tggaaaggaa tgttcgatat agaaagaaaa ccatttgcta
     1741 acaatcaaac ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga
     1801 tccatgaaat tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaatga
     1861 caacggatac tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg
     1921 atggtcaaga aattatcaat ggtcgtgctg aaaaaacaat caattctaat actgaaattt
     1981 ctttttgtat gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa
     2041 cagcctttgg tgatattgaa acaagtatta aggaacttag ggaatatgtg cagaacccat
     2101 ataattttca atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa
     2161 attataaatc aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat
     2221 tttactgtag gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta
     2281 tttagtcttt atgcttggat tgtacaaatg gtttgggttt ccatattgat gctgttgttt
     2341 tattggatag tacgggttat tcttttattg ttaaacaaaa gaaaaaagga taggtgaata
     2401 aaatgaaatc aactaaattg aaggttgtat tgttatcgac agcagcatta ataaccctta
     2461 cagcatgtgg tacaactaat agtgctaata ataacaaaga cacaaagtct agtagtacga
     2521 tggttactaa aaacagcact acatcggcat caacaacaat gtcttcaatg actgaaaata
     2581 atgctgaaaa gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gatgaagttt
     2641 ataacaactt tattaataca gtaaaaaatg atgaaaatta tgggaatgat ccattgcaaa
     2701 ataaaaatct gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat
     2761 caaaaatcat gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata
     2821 ttaccataaa taaaccagct tacgagtatt acgcagttgc tgtagatatt actgatggtg
     2881 tacctgagtg ggtacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc
     2941 caacagttgc agtagtaaca ccaaagaaag ttaacggcac actatactgg gatgtaatgg
     3001 gaatgacaac aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt
     3061 tgggagcata taaattatat caacaatcag agcctgttat ggacgatcaa tccaaagtta
     3121 aatagatctt tagggcgtaa gccctatttt tttaaaatta agtgataaca aataaatgat
     3181 ttctgaaaca aacgttctta ctcaactact cgaaacacac aagctaatta actagattat
     3241 aaaggagaat gcgtaatgaa agaaatcagt gcaaatgaag ctcatattcc gcaaaagaat
     3301 caggttggta tatataaggt gcttcatggt gaaggaattt ataaaataca gttacttacc
     3361 ggttacaata atggtgcaaa tgggaagtgg ttaaaggcaa caagtattcg tgaacttaga
     3421 agtgaagtag aaaaaattag gcttggtctc acaagtgaag aaatagctaa actgaaggga
     3481 aataattctt ttgtaattga aaaagaaaag gaaaaacatc tactttatcg tgagctgaaa
     3541 attgaagaat actatgaaat tgatttacct gaaaaattaa gaattattga tcaacgttca
     3601 gaagaaaaat ggaaaactga aatatatcca agcttaaatg actacaagac atcaggaaag
     3661 cccatgtata actacacagg ctttttaacc tacacacaat acaaatatgc ctttcgcaaa
     3721 ttaaaaggca ttaaacaagc tgaaattgcc ctgtatgaga tgaaaaaagt ggatagattg
     3781 aaattgattc aagatttagt gcaaaataca tggaatcaag cgattgtcga gctagaaaaa
     3841 gaaaatggga aaattttaac taataaatat ctgatcgtaa atgaaattga agaattaatt
     3901 ggtttatttt catgtgaaaa aggtgaagtc ttcttgaagt catcaccaac ctattcaatt
     3961 ttggacagtc cgtatcagtt tatacaggca aggagatgag ttagatgtct agcttgaaag
     4021 atgactatat ccaagcaaaa caagagtatg aaaaaataca gatgttgaaa tctgagaaag
     4081 tcgaattatt tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa
     4141 ctttagagga atatgatcgg cagtttaatc agatacatca caatatcatt ttgcttgttg
     4201 agcaggcaaa gcgaaggttt gaaaaagcgg aatatgatct attagatcaa ggaattgtat
     4261 gcttggataa gggattgcga agatattatg gtgcagagtt aaaaaagcaa aatgtagata
     4321 gaaatgcact aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa
     4381 agcaaatgtc acaaaacatt agtgctgtaa aacaattaat agaagaagat ttagagacca
     4441 cagttcagag attagcggaa cgaatccatt ttgcaaatta tcaaaccatc tctaactggt
     4501 caagcaggaa cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag
     4561 aatatggtgt tgatccgata aaaattttcg agaagaccat gatttattac tatcatggaa
     4621 cacctaacga agtgttagaa catcttttgg aagaggcaaa agaaaaatca tcaatattag
     4681 agctatcgca aaatgacaaa gttattcaag tgaaatgcgt tgatccaaca ggaaagatta
     4741 ttgtcggttt tgatattgaa agaaaatcca aaatagattt tattacacaa ttaaaaaaga
     4801 taaataattt agatattcta acgcttagac cagatcatga acagttgttt atgagtttta
     4861 aaaaacaatt gaaagaaata caaaaaagat ataacaaggt gtttcctaat gcgtgaaaag
     4921 ctcaaagaaa tcagagtttt ttggataatt aacaaagaat gtatcatctt atatactctt
     4981 ggatttctta gcatgatctt gttttggtgg ttatatttaa aacagtaatt ggagagggtc
     5041 aagaggattt ttatcaagat aaggatgtgg aaactgcatg tggaataata gattaaaaac
     5101 aggattgtta ttaatagtta tttcctgcgc tatgatgatt ggtatgagga tacaaaggga
     5161 acaatcctat tttgaagttt ctgctaacaa tgtaatagaa agatgccatt atggtcaaca
     5221 tttttggact ggggaagtca gagaaaatat agatcgtgaa tatgttcaaa gaattgtttg
     5281 ggatgcatat tctatcaaag attatcctaa aagtttaacg agtagattat tttacagtga
     5341 aaaagataat caaaaactct cagatttgat gatgaaaaaa gtatgactat tattacgaaa
     5401 gagaaacaga gtgctatatt cgaggggaaa aagcctacaa caaaagtgac tattggggtg
     5461 acaaggcgaa gcgattaact gcaaatttta gagtgtatga atcagagttt ataaacttga
     5521 cctttcttaa tagtgttttt attgattatg taattactac gaaacgcatt ggaagttatt
     5581 caagtaccta cgcatcctta gttccaatgt taaactcgat gagccgtttc ttaaaagaaa
     5641 gagaaaagaa agagctacag tatatcaaag aaatcatgcc ggattttgaa atgcacgaat
     5701 ggcaaattgc tttatcagat tggaaattgg cgaataaggt tcgtagaata acaccttttc
     5761 aagccaagcg ctttttgaga gctatgaaat ttgtgcaata aaaaaagcca ccagacaagt
     5821 tgatggagac taacacaatt accaacagtt gtaattgtgt tagcttacaa attaattatg
     5881 gtgtttagtc aatcccaaat tgtgaaatgg caccaataat tggaaaggaa aaatgaaatg
     5941 tctgaagatt tacgagagct gaagttacga aagaagcagt atgatcagta tttttccggt
     6001 ttgttgaata aacatattga agaaggactt gatcatgatt ttggaaaaga tgaatattgt
     6061 ttatattgcg aacgttacaa tgaaaaaatg tctgaatatc tacagctgaa aaaagagata
     6121 cagaaattag agaaaaaagt acatgcttgg aagtataaag attccatgtc tggttatgtt
     6181 gaatctcaaa ataagccaag aaaaaagaag caagcgtatg ttctaaagat ggtaaatggg
     6241 gaagagttaa ttgtattagc gtacagtgaa gaagaggttc gagaactcgt tgaaacacct
     6301 gtcaggaaaa aggttgaaac aataaatgag atagacgaca gccaatacaa tagcatctat
     6361 attgaaacaa gtcatggtgg atataagaaa ttagcaactt tccttagaag ttctaaagtt
     6421 gggataattt ctaaaaaaga cataagtgct gttaccgata gtttttgtac tttatctgtt
     6481 cctaaagctt atttccaata cgatagtaaa agatttgaac gcttagagaa attctgtaga
     6541 ccgtatactg tattgaaaac taaaaaattt aataaaacta aaagagtttc tatacagtta
     6601 ccgactggtc aaatctcatt aggtgaaaaa gactatctga taaaagtagg aaaagagtta
     6661 attataaaag taccggaaca cttatttaaa gaaaattttg agatacgtga aaccttggta
     6721 tggaaagaag aaaaatagtt tggaagacat agaatggtat aaggggaaat tgaaatggca
     6781 gtaaataaaa tattgcaggt actaatcata gacgaatttg gcggaggaga atggtttgat
     6841 gtgcagtcca tagactttga agaagaagtc gtagttatag aaaatattta cggcgaagta
     6901 tattcatatt cgcttgaagg tgagaatatt gaggatttac ggttgattgg tgagtagaaa
     6961 aaaacagaaa tatcaaaatg gagataaaaa ttaatagcat gtaaagatag tcatgcttgg
     7021 gttaaaaatg ttaaaattca aaaaaatgag tcagaaggga taaaagtatg aaaagagagg
     7081 aatacaaaca aagattaaac gaactgcttg aagaggacga aacattaact catggttcgc
     7141 ctgatgaaat tctatacatg attgataata tggtgatctt tggtggatat gaacttggta
     7201 ataggtcagt tgatcataat attttagaat ttgatgatgt atcatgggaa gaaattcttg
     7261 attggggaat tttggcggta cctgaaacta aaacctatat tagtgacact atggttcctt
     7321 tttttgagga attggattat aaaagacttc ccaaaaatga aaatcatata ctgggaggaa
     7381 actaacaatg ttaagtgact tatttatttt actgatctat ctaggtgtaa gtgaaattat
     7441 tgcgtatttt ttgtttgtca ttgctaatta tggtatttcc agtgcaatcg ttcaaatgtt
     7501 tagattacca gtaagtgaac taaaaaaatg atgagattat cagcatatct tttaatgttt
     7561 ttgaatgtgg tcacgttaat ctttgtagta atcggtgtga ctttcgattt aatggaatag
     7621 tgggaaatct ataatggaaa aaagagacac gaatgaacat attttttgaa tcaatattgt
     7681 tgatggtttt tgcgatcagc gcttaccaga aatctaaaaa agagtaatga ttgtttaatc
     7741 atcactcttt ttttgtaggg aaattgaaat atgtggagct tgtattggtt tagttaggat
     7801 tgcttggcta taaggagcta gttctttatc aaaaccatca aagtaggttt tcagtagcga
     7861 tttaactgat cgctcatctt gtttgatttg gataatttca gcatccaaat cggttgtgag
     7921 atcaggagct tttgatgcga ttacctgatt ttttttatct tccaactctc ttaggctgtt
     7981 taacaagttg taaaaagaat cattttgatt gttcattcca acgtgagttg caattttaac
     8041 agaagtttcg tccgtattgt catcaaaaga actcattgga atatcaagta gtaacgagga
     8101 tgtttctttt actgactttt tctttacagc attttttgtt tttccatctt ttttgctctt
     8161 tactcgagat gtttttggag ctagtttagg taaatcactt gttttatcag tcatttatta
     8221 gcttttcctt tctattaatt tatgttatta tattatcata ataacataaa aaagtacact
     8281 ttttgggggc gtagttattg aaaaaatcta agagaaagat tgccacattg cttatgttaa
     8341 tggtgcaatt tacagggatt gcaatgagtg taatcacaat aaatccgcaa gtgatacatg
     8401 ctgatgggat agctacttat gatgcacaat ttaactgtac tactgaaggt agtgggtcag
     8461 gaacagatag cgatggaaac gatactggaa gtggcgctgt aacaggtgat gtagccaaaa
     8521 atattgaaga tatttacaaa tacatgaatg gagaatatgg cttatcaggt gctagtattg
     8581 caggtatttt aggtaactgg aaacaagaat caggtattga tcctcgagca gtagaagggt
     8641 attacggaga tggacagaga gaagttgact tcatcaaaca atttactgat ggtctgatgg
     8701 gtattggcta tggtcaatgg acagctgggc gacatacagc attagttgat tggtctaaga
     8761 aaaataatgg cggttcttgg tggactacag aagcccaatt ggactttatg ttcaaaggtg
     8821 atgggggatt cgtaggaata ttaaaagatt atgctttaaa tgcttctgat gatgtcgtag
     8881 aaaatgcagt aacttttcat aaaaactggg aaatttcagc tgattccgaa tcagctgtaa
     8941 tagctgtacg tggtgaaaat gcaaaagaaa tctggaagta catgaaaagc aagggtatgg
     9001 acggtgcaaa agatgaaagt aagatcaata aaatatccgg aggttcaagt accggagatg
     9061 gatcgaataa ttcatcaagt tcagcatcag gaacagtaga agatgcctgt gcaactacgg
     9121 atgacgctaa ttctggttct ggatcaattg ggaaagaagg aaaaattaac ggtaaaaaag
     9181 gtcaagtaat agcgaatatg acttatgaag aagctttgaa aaaatatggg aaagcgatca
     9241 cgctaccaaa attcaaagaa ccagattggt caacttcacc ttttaacgga atatatcaag
     9301 gtcagtgtac agagttgacg tgggcttata tgagccaatt atacgatggg aatcaaccga
     9361 ctactggaaa tggtggttat gtatgggaaa gctacaaatc ggcaggagca aaaataacga
     9421 acaaaccaat cgtaggttat gggtttagtg cttcggatgg atggttatgg gctgttggag
     9481 atgcagggca tactggtgta gttatagcgg tgtttgaaga tggatcattt ctatgtgcta
     9541 actttaatgt tccaccaacc caagctccaa caaggggagt ttcggtaacg ttgattgatg
     9601 gagtggacgg atcagataat gtgcatttct ttagcggtgt aggaaaagct aaatttgaaa
     9661 tcgaaaaata aattggaatg aaggacgata tagatgaata gtaaagtgaa agatacaatt
     9721 atcatttctt tgctttctgg tatgttatta gcactgcttg ttggaatcct attcttcaat
     9781 aagcagaatc aggacataga agctgaaaaa cacagtgtgc atattgaaga taagctgaca
     9841 ttctttaaag aggttaaacc aaatgaggtg gatggattac ggaaagattt agaaaatgat
     9901 attcaagaaa tgttgaatgg atcagttcaa aataagtttg cagatcaatc aggtgaagaa
     9961 tttttgagaa aactatttat tgtaacaggt aaaccaccaa ttactgataa gagtagtgag
    10021 aaagaaaaaa tcgagtacta taagcctttt acttttaagc taacaaactt tggaattaag
    10081 acatctagta gtgggttgta tgaagcattg accgattttg aagttcaata caatggaaaa
    10141 aaagtgtatg ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt
    10201 gctttttatg caaaatcatg aaatcactac tgctaaaaag gcgatcggaa tactttccat
    10261 aattgtgatc ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt
    10321 caaagaattg gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt
    10381 gaaagctatg caggaagctc aatacaaaga attgggaaat gctgaaatta aggaacaaag
    10441 taggaaattc tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat
    10501 gaaggaaata caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatttctgg
    10561 cacgatggta ggcactggta atagtccaat atctagctat actgctgatt actatacaac
    10621 aaatcaaaaa ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc
    10681 aacaacaaca atttggcgtg gagtaacaag cataggagaa aatcaattgt tttatttgga
    10741 ggatttagat agctatgtaa gagcagagta aaataaaaaa agagctaacg gtaccaacgt
    10801 tagctctttg tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa
    10861 cacaaacaga aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat
    10921 ttagattata ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa
    10981 agtcaaaagt aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg
    11041 tttatttga
//
LOCUS       DAXWLH010000083        11019 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXWLH010000083 DAXWLH010000000
VERSION     DAXWLH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.083"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..662)
                     /locus_tag="ACGE36_001717"
     CDS             complement(<1..662)
                     /locus_tag="ACGE36_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8384105.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QL"
     gene            796..1083
                     /locus_tag="ACGE36_001718"
     CDS             796..1083
                     /locus_tag="ACGE36_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384106.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1499..3037
                     /locus_tag="ACGE36_001719"
     CDS             1499..3037
                     /locus_tag="ACGE36_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFF8384107.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3034..3999
                     /locus_tag="ACGE36_001720"
     CDS             3034..3999
                     /locus_tag="ACGE36_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384108.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3986..5308
                     /locus_tag="ACGE36_001721"
     CDS             3986..5308
                     /locus_tag="ACGE36_001721"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8384109.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5371..5475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5549..6292
                     /locus_tag="ACGE36_001722"
     CDS             5549..6292
                     /locus_tag="ACGE36_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384110.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6307..7299
                     /locus_tag="ACGE36_001723"
     CDS             6307..7299
                     /locus_tag="ACGE36_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8384111.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7311..8048
                     /locus_tag="ACGE36_001724"
     CDS             7311..8048
                     /locus_tag="ACGE36_001724"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384112.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8038..8760
                     /locus_tag="ACGE36_001725"
     CDS             8038..8760
                     /locus_tag="ACGE36_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8384113.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8844..9467)
                     /locus_tag="ACGE36_001726"
     CDS             complement(8844..9467)
                     /locus_tag="ACGE36_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8384114.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            9802..10236
                     /locus_tag="ACGE36_001727"
     CDS             9802..10236
                     /locus_tag="ACGE36_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8384115.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            10259..10579
                     /locus_tag="ACGE36_001728"
     CDS             10259..10579
                     /locus_tag="ACGE36_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384116.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            10720..10911
                     /locus_tag="ACGE36_001729"
     CDS             10720..10911
                     /locus_tag="ACGE36_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384117.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT         3696 a         1863 c         2325 g         3135 t
ORIGIN      
        1 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
       61 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
      121 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
      181 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
      241 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
      301 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
      361 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
      421 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
      481 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
      541 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
      601 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
      661 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
      721 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
      781 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
      841 aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg
      901 gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt
      961 gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag
     1021 tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata
     1081 taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt
     1141 ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc
     1201 ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt
     1261 aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt
     1321 gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc
     1381 atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt
     1441 tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat
     1501 gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca
     1561 acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc
     1621 gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct
     1681 ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac
     1741 tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta
     1801 tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc
     1861 cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg
     1921 tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc
     1981 aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt
     2041 gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt
     2101 gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg
     2161 cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa
     2221 attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac
     2281 aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact
     2341 ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt
     2401 gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc
     2461 agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa
     2521 tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc
     2581 cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg
     2641 ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag
     2701 catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc
     2761 ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg
     2821 gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc
     2881 tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga
     2941 tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct
     3001 gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat
     3061 gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa
     3121 ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg
     3181 atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat
     3241 gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg
     3301 ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa
     3361 aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat
     3421 atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc
     3481 atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta
     3541 gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc
     3601 acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt
     3661 gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat
     3721 cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga
     3781 gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa
     3841 caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc
     3901 attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct
     3961 tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg
     4021 tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt
     4081 cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt
     4141 gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga
     4201 gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt
     4261 cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc
     4321 tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt
     4381 agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa
     4441 aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg
     4501 tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat
     4561 tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt
     4621 ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat
     4681 ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat
     4741 tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat
     4801 cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat
     4861 ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt
     4921 tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc
     4981 gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag
     5041 tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca
     5101 tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat
     5161 tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat
     5221 caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc
     5281 aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt
     5341 tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca
     5401 tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga
     5461 aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta
     5521 ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat
     5581 ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat
     5641 tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc
     5701 ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc
     5761 ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt
     5821 actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt
     5881 tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt
     5941 agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac
     6001 gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg
     6061 cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga
     6121 atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca
     6181 gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg
     6241 cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg
     6301 gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc
     6361 actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt
     6421 ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat
     6481 tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt
     6541 ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat
     6601 gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat
     6661 acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga
     6721 aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa
     6781 aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa
     6841 agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca
     6901 aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga acggttagat
     6961 tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta
     7021 cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac
     7081 agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca
     7141 caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa
     7201 aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta
     7261 ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc
     7321 aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca
     7381 ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat
     7441 tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc
     7501 aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc
     7561 cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca
     7621 atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt
     7681 tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga
     7741 atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag
     7801 ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg
     7861 tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc
     7921 gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc
     7981 tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg
     8041 ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa
     8101 cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag
     8161 cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc
     8221 tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg
     8281 ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag
     8341 cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa
     8401 gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg gtattcttct
     8461 ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg
     8521 aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt
     8581 gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat
     8641 tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt
     8701 gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa
     8761 aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa
     8821 actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa tgctttcgcc
     8881 gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc ttggtttgca
     8941 gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat gtaacgtccg
     9001 ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt tgcagagctt
     9061 gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt ttcagcaaca
     9121 ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc agcttggact
     9181 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
     9241 attgttaatt gttgtcctga gtaaatcagg ttaatatcag aaattgaatt tgattctgca
     9301 atggcattga tcaaggagtt atcaccaacg tatttttgag aaatcgttga tagagtatca
     9361 cctgattgaa cggtataagc ttcgtccgca tgagcagtcg ttcccatgaa gaatgcagcg
     9421 ccggcagcca aagttgttcc aaaaagtaaa gttttaagtg atgtcatgaa aaatctcctt
     9481 tattcgttaa aaagttttat ttgttgtgtt caacgaggaa tactttaaca cgtgaaaata
     9541 tttcataggt aaaagaagca tgacaattta tgacaaatcc acgttttttg taacagaatg
     9601 atgttcgctc gatgtgaaaa atgatgagga atttcacata atgcagaaat aatgggatta
     9661 gaacaggtgt tctgatgaga aaagaaatgt ataaataact tgaaagaaat tgcttttgtt
     9721 catcatttta tttacatatt gcgtatattt tttgtatttt tgcttgagat gttacaataa
     9781 aagaaaaagg aggcggttcc aatgagtaaa agataccaga acatcatggt agcagttgac
     9841 ggatcaagac aatcgatcaa agcgttcgct gaagcccttg acttagcaaa ggacaatgaa
     9901 gcccacttat ttatcgtgtc aatcatcaac aaagttgagc tgacccatag tgcctatgcc
     9961 ttttcaaaaa tctatgcaga tgaaaaacaa agaacagaag tagccatgct gaaaaaaatc
    10021 aatgatgcca aagagtacgg cattgctgag atccacgcaa tagttgaaac tggagatcca
    10081 agaaatttga ttgccaatgt catccctgga caagaaaata ttgatttgat cgttatggga
    10141 gcgacaggaa aaggtgctat acaacaagca ttagttggat cgaccgcttc ttatgtcgta
    10201 tcacatgccc cttgcagtgt gttggtcgtg aagtaatgag ataagggagg aatagaaaat
    10261 ggttcgtcct caagaagtcc gtgcgccaaa agaaaaaata gaaattttag caattattga
    10321 agacggcaca caaacaaaga agggatactc gatcgcttta gtaaagtgga aaggaaaaaa
    10381 aggtgttgct ctgagatggg atggagataa ccaacaagat aaaggattcc ctgtcacagc
    10441 taacggttat catccagcgt ggtttgttct gcctgataaa tttatggaat tatacagcta
    10501 tgattatgca agagccgcag catcaatcaa agcattagat aaattaacag aagagcagaa
    10561 tgaaatggag gaaaaataaa aaagacgaac caatcagcct ttagaaaact gattagttcg
    10621 aggagtaaaa atgaaaaaag gttatttggg atgaaactag aataatgcgt ataacttaaa
    10681 taaaacttaa agcaataaaa tggcaagagg gtgaggcaaa tggaagcagg aaaagtatta
    10741 caggaagtaa aagcactatg tgcagataaa aaatatgaag aagcgaaaag cctgatcgaa
    10801 aagaacaaag aaaatttgga agaaaagtac ccgatcgcac aagaatttat agaattaaaa
    10861 caaggaacga tccttcaccg attcaaacat ttttttggta taacagaata gaaaaaacga
    10921 ttaaagatcg gcagaaattt gctgcatctt taatcgtttt ctttttaaac atgcgaggga
    10981 ttcaattctg attcttatat aaaaaataga tccaaataa
//
LOCUS       DAXWLH010000084        10919 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXWLH010000084 DAXWLH010000000
VERSION     DAXWLH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.084"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..634
                     /locus_tag="ACGE36_001730"
     CDS             <1..634
                     /locus_tag="ACGE36_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8384118.1"
                     /translation="AIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKG
                     IFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAA
                     MVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQV
                     LDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            646..1566
                     /locus_tag="ACGE36_001731"
     CDS             646..1566
                     /locus_tag="ACGE36_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8384119.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1607..3064
                     /locus_tag="ACGE36_001732"
     CDS             1607..3064
                     /locus_tag="ACGE36_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8384120.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3409..4023
                     /locus_tag="ACGE36_001733"
     CDS             3409..4023
                     /locus_tag="ACGE36_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8384121.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4016..5740
                     /locus_tag="ACGE36_001734"
     CDS             4016..5740
                     /locus_tag="ACGE36_001734"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8384122.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5737..7188
                     /locus_tag="ACGE36_001735"
     CDS             5737..7188
                     /locus_tag="ACGE36_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8384123.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7661..9814)
                     /locus_tag="ACGE36_001736"
     CDS             complement(7661..9814)
                     /locus_tag="ACGE36_001736"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFF8384124.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIRQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9816..10862)
                     /locus_tag="ACGE36_001737"
     CDS             complement(9816..10862)
                     /locus_tag="ACGE36_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8384125.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
BASE COUNT         3582 a         1923 c         2161 g         3253 t
ORIGIN      
        1 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
       61 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
      121 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
      181 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
      241 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
      301 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
      361 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
      421 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
      481 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
      541 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
      601 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
      661 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
      721 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
      781 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
      841 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
      901 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
      961 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
     1021 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
     1081 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
     1141 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
     1201 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
     1261 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
     1321 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
     1381 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
     1441 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
     1501 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
     1561 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
     1621 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
     1681 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
     1741 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
     1801 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
     1861 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
     1921 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
     1981 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
     2041 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
     2101 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
     2161 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
     2221 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
     2281 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
     2341 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
     2401 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
     2461 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
     2521 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
     2581 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
     2641 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
     2701 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
     2761 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
     2821 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
     2881 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
     2941 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
     3001 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
     3061 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
     3121 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
     3181 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
     3241 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
     3301 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
     3361 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
     3421 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
     3481 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
     3541 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
     3601 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
     3661 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
     3721 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
     3781 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
     3841 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
     3901 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
     3961 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
     4021 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
     4081 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
     4141 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
     4201 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
     4261 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
     4321 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
     4381 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
     4441 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
     4501 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
     4561 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
     4621 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
     4681 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
     4741 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
     4801 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
     4861 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
     4921 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
     4981 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
     5041 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
     5101 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
     5161 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
     5221 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
     5281 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
     5341 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
     5401 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
     5461 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
     5521 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
     5581 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
     5641 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
     5701 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
     5761 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
     5821 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
     5881 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
     5941 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
     6001 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
     6061 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
     6121 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
     6181 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
     6241 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
     6301 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
     6361 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
     6421 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
     6481 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
     6541 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
     6601 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
     6661 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
     6721 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
     6781 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
     6841 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
     6901 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
     6961 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
     7021 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
     7081 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
     7141 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
     7201 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
     7261 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg gagttcaaaa gccagtttct
     7321 cggtattaag ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa
     7381 tactcgaaac taaacagctt tttcacgacc tctccactaa acggtgttca atcagctgac
     7441 cctcggtatt agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct
     7501 aatactcagg tcagaacgct gatttcacaa cctctcaata aacggtgttc aaaagcttgc
     7561 atctgcggta ataagaccaa ccaaacgaaa aatatgagga tctatttttg cttggctgtt
     7621 cttattactt gatgcaggac agctttttca caacctcttg ttaattatag tttgataagg
     7681 aaaaaggcaa atcttttgct tgatatttct tagggtgagg gatgatccct aattggtaag
     7741 agatcgtgcc gcctttcagc aaatcttgat gggtaaaata taatttcgta tattctttgt
     7801 ccatccgttt gattttatgg ataaattgtt gctgcggccg attaggcgtt gcttcaatcg
     7861 tcaattcctt tccatttgac aaaagcaaaa cagccttgtc aattaatggc ataccgataa
     7921 catattcacc ggttccaggc gtgacaggat aaaatcccag actattgaag atataccaag
     7981 cggccatact tccattgtcc tcatctccag ggtaacccat tggagaatcg tcgaaggtct
     8041 gagtcatcaa ctgtttaagc agtggctgtg ccatttcagg ttgtcttata taactgaaca
     8101 agtaaggata gtgaaaactt ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg
     8161 ccatctcact catttcgtgg atctcaaaac catatcctcc tgggtcaaac gttgggggct
     8221 gattgcacag ctcaatgagt ttttctttat atctctcact gccaccatat tttccgatca
     8281 gtcctgaaaa atcgtgataa acagcaaagc tgctttgcca agcacttcct tctgcataat
     8341 cttgtcccca acgattagct gaaaatggtt ctctgaactt gccatcttta tcttttgaac
     8401 gcataaatcc tgactcatag tcaaagatat tttgatagtt caatgattgc tgctgatatt
     8461 tcttcgtcat ttcctcttct tctagtacct tggcaacttg gctaatacaa aaatcgctat
     8521 aagcatagtc taatgtgtga ttgactgatt cgtggtagtg attaggaaca tagccatatg
     8581 tcaagtaatc ttctgttcct tggcgtccat aatttggatg tttgctttga accgtagcac
     8641 ctttttccat tgccgttaga aattcaggca tcaagtctcg tcggatatct tttacagcag
     8701 catctgcaat gaccgcatcg atcaatgtcc caggcataag tccacgttca tcaggagaca
     8761 gccattttgg caaatagcca ctttctctat agctgttcaa aaaaccttcc agcatttctt
     8821 cgtatttttc tacagcaatc aaagaataaa gaggataaac cgttttatac gtatcccaaa
     8881 acccattatt tgtatagaga acaccaggtt ttatagatcg actagtcgta tcatagtgga
     8941 ttttttgccc actaggatca atttcataaa atgtttgtgg aaataaaaac acccgatata
     9001 aattatgata aaacgtccgc aattgttgtt gatcatggtg tgtgagctgt atacgatcaa
     9061 aataggactg ccattgttct tgactgtgag caaggtaagt ctctttgctt tggttttgtt
     9121 ctagttccag gttataagct gcttgttccg agctgataaa agatgtccca aatcggatag
     9181 tttgtttttt tgtctgttca aaatacaacg ggatcgagtc gctatttttg ttggaaacag
     9241 ctaactcatc aattgttaaa ggatattcaa atctcaatat aaaataaaaa gaaaaatcag
     9301 gatcttctga accagcaaaa tgtgtgattt ttccttccac tgtgtaagga tctataacaa
     9361 agagctgatg gcgtcccgga aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa
     9421 tagataagat tccgccatac atgctaggta tcaatgtaga acagatgcca taacgtaatt
     9481 gacagatcga cagatgagtc gggcaaaata tactttcttc tgggcggtaa gaactttgag
     9541 catggaaaag ggtgttctcc tgtagttttc cattgatcgg cgtaaacagc atatgactga
     9601 aatcacccat ccaaggactt ggttgatggg taagacggta gccttgaaaa acgcggtcat
     9661 caggatgaaa ccaccagctg ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc
     9721 caaaaggaac gccagtataa ggctggcagt tcccattaga gaaatttggc tgattcgctg
     9781 ttgcatgtct agtatctatt tgaccgatat tcattttatt tctcctttgt tgattgtctt
     9841 ttgatgaatg aaactggaaa tttactatcg gatggtcgat cttttgtctc taaccaagtg
     9901 accaaagcat ctcccgcaag cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt
     9961 ggcgggtcaa caagagctgc tgcttggata tcatcaaatc cgataattga aaaatcttca
    10021 gggatatgat aaccatgctg tctcaagaga ttcatggtct ggatagcgac aacatcgttt
    10081 tcacaaacaa aagcagttac ttcatactta gtgatataag aaagcaattc atctaatgtc
    10141 gcgcttttgt catccagtga agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg
    10201 taaccaaggt aacgctgtcg aacggattgt ggatgagtct gcgtaccaaa caaataagcg
    10261 atttttgaat gtccctgttc gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta
    10321 tcggaaagaa cggtaggaaa aggtaactca tagatttttt tatctaatat gacgacaggg
    10381 aattctttca atgaaagctc aaaaagtaag tcaagatgtt gttctgtatc aagagcataa
    10441 taaatcaatc cgtcaaattc attgattagc tcttttgctg ttttattttg tagaaagtca
    10501 agcgtcgtca tcatgacatc aatctgttta tcttgcatca cagggttcaa tcccttgcta
    10561 aaatcaccaa cagacaaatc agcgacatat ggaagaagaa acagaatccg tcgtgttttt
    10621 gtaggagaag caagaatctg ttccggagaa gcggatttaa cgaaactgcc tttcccgcga
    10681 acgcgataaa taagtcctaa ttgctctaat tcagtcaatg cacgtttgga agtgattctg
    10741 cttactttat aggtacgtga aagttctttt tcagttggta gttggctgtc tacaggtaat
    10801 tcaccagtta aaatagccgt ttgcaaatca ttaaatattg tctggtacaa tggttgattc
    10861 atataaacac ctcttaatga tatatcatat tcaaataata ccataatgga tgaaaaagt
//
LOCUS       DAXWLH010000085        10864 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXWLH010000085 DAXWLH010000000
VERSION     DAXWLH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.085"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..523
                     /locus_tag="ACGE36_001738"
     CDS             <1..523
                     /locus_tag="ACGE36_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384126.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2053
                     /locus_tag="ACGE36_001739"
     CDS             584..2053
                     /locus_tag="ACGE36_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8384127.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2064..4173
                     /gene="topB"
                     /locus_tag="ACGE36_001740"
                     /pseudo
     CDS             2064..4173
                     /gene="topB"
                     /locus_tag="ACGE36_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            4189..4338
                     /locus_tag="ACGE36_001741"
     CDS             4189..4338
                     /locus_tag="ACGE36_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384128.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4419..5015
                     /locus_tag="ACGE36_001742"
     CDS             4419..5015
                     /locus_tag="ACGE36_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8384129.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            5028..5927
                     /locus_tag="ACGE36_001743"
     CDS             5028..5927
                     /locus_tag="ACGE36_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8384130.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            5930..6415
                     /gene="ssb"
                     /locus_tag="ACGE36_001744"
                     /pseudo
     CDS             5930..6415
                     /gene="ssb"
                     /locus_tag="ACGE36_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            6437..6694
                     /locus_tag="ACGE36_001745"
     CDS             6437..6694
                     /locus_tag="ACGE36_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384131.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(6853..6975)
                     /locus_tag="ACGE36_001746"
     CDS             complement(6853..6975)
                     /locus_tag="ACGE36_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFF8384132.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            7130..7389
                     /locus_tag="ACGE36_001747"
                     /pseudo
     CDS             7130..7389
                     /locus_tag="ACGE36_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7837..8043
                     /locus_tag="ACGE36_001748"
     CDS             7837..8043
                     /locus_tag="ACGE36_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8384133.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            8043..8294
                     /locus_tag="ACGE36_001749"
     CDS             8043..8294
                     /locus_tag="ACGE36_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384134.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            8310..8747
                     /locus_tag="ACGE36_001750"
     CDS             8310..8747
                     /locus_tag="ACGE36_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384135.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            8740..9435
                     /locus_tag="ACGE36_001751"
     CDS             8740..9435
                     /locus_tag="ACGE36_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384136.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            9600..9800
                     /locus_tag="ACGE36_001752"
     CDS             9600..9800
                     /locus_tag="ACGE36_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384137.1"
                     /translation="MNKIIPLLLVLFVGEILMRLSAAVHLNLAVFLVILMFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            9816..9995
                     /locus_tag="ACGE36_001753"
                     /pseudo
     CDS             9816..9995
                     /locus_tag="ACGE36_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10057..10241
                     /locus_tag="ACGE36_001754"
                     /pseudo
     CDS             10057..10241
                     /locus_tag="ACGE36_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            10275..10706
                     /locus_tag="ACGE36_001755"
     CDS             10275..10706
                     /locus_tag="ACGE36_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8384138.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         3935 a         1832 c         2281 g         2816 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc
      241 tgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt
      361 aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaattca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgct gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg
     1081 tttagatcat gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc
     1141 aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca
     1201 cattgacaag aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca
     1261 tttaaaattc tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa
     1321 actacaagat gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta
     1381 cgttgaagag tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat
     1441 atgtgtggac aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat
     1501 cgttgatcct tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca
     1561 agtacctaaa gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc
     1621 tgaagcaatt atggccatac acaaaactga aactaattta cagctgacaa atcaattggt
     1681 gtctgctcat gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga
     1741 aacaattgat ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg
     1801 cgcagatgga acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt
     1861 taatgcagga atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca
     1921 tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat
     1981 ccagcaacaa gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca
     2041 agaaagggag tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag
     2101 cgaaggctta tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc
     2161 aagatcgatt gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt
     2221 tagattcgcc cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta
     2281 ttttccccaa tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg
     2341 tcaaacgaca gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag
     2401 gggagctgat tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta
     2461 aaagactttg gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac
     2521 gagacgcaga agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt
     2581 ggttaattgg gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac
     2641 caggaagttt ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc
     2701 aggaaaagat tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg
     2761 ctcaaaatgg cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag
     2821 catttgaagc ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc
     2881 ttcaagtcga aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc
     2941 ttcagtctaa aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc
     3001 aagggttgta tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg
     3061 aaggggaata tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg
     3121 aagcgattcc gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc
     3181 aggaacatta cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac
     3241 taagtccact gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg
     3301 cggaaaagta tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa
     3361 aggcaatcga aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg
     3421 acggaaaaga gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga
     3481 cggtggatct acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta
     3541 cgttgatcac tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca
     3601 tattgaaaga agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct
     3661 tgaaacaaaa ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga
     3721 ttttgctttg ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca
     3781 aatgggaaag ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag
     3841 ccaatatcca aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca
     3901 ctgattttgg atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc
     3961 gtcatttagg aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg
     4021 cagcttgtac gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg
     4081 attgttttaa aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac
     4141 ttcaagaaaa gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc
     4201 acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa
     4261 atgcgtgcca gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa
     4321 atcgaattag atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact
     4381 gtttttataa gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca
     4441 agctgctaga agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt
     4501 ctcctgtaac gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt
     4561 ttttcacctt acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac
     4621 gcggcattat ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta
     4681 gacagatcga tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat
     4741 tcgcagttga gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa
     4801 aaagtcgcta tgggaatcct attatagctt atgatccaga aagaacttta tgtgacattt
     4861 ggaatccctg gtataccgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactatatgg
     4921 agtcggacag aaaaaatctt agaaaattga atgagtatcg acgaatttta ccaacagaca
     4981 aaacgatgcg ttcttatatt atggctttaa attaaaggag aagatacatg acacctacgc
     5041 agttaaaagc aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt
     5101 taagacattt tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa
     5161 attttgtgtt aaaaggcggc ttcttgattg gctcaaagta tggcattgaa aatcgcacaa
     5221 ccaaagatat tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttaacaacgg
     5281 ttctaaacga aattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa
     5341 tgaaagaaac gagagaagcc gattactatc caggatttag cttacgtgtg ctggcacatt
     5401 tggaaaacat gcggccagat tttaaggtag atgtgactac aggtgactcg atctatcctg
     5461 caacgatcac tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct
     5521 atcctacaga acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata
     5581 attcgagagg aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg
     5641 agaagacctt gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt
     5701 taaagatata ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata
     5761 cctggaagaa atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa
     5821 tggattcgat tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac
     5881 tagcttatca aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa
     5941 tgtgacatta gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac
     6001 tgcagtagcg acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg
     6061 agaagccgat tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt
     6121 agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa
     6181 ccagcaagga caacgtgtat atgtgactga agttgtagca gataactttc aactattaga
     6241 aagtaaagca gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac
     6301 cgccaccttt gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca
     6361 gaatccattt ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga
     6421 gaaagaagga gtaagcatgg caaaaaaaat tagtctgacg atggatctat tgatacccgt
     6481 aattgcattt atagacggga tttctttggg cgtcactgaa aatcctttgg taaatagaat
     6541 tgcgaaagga tcactatttc ttgtattcgt gtatcttgtc tgttcttatc ttttaaaaag
     6601 agcaggaaag gataattcct tttggcgatt gactaaagtc acagtagcag cgacagctgt
     6661 tatacttacg gctattctac cgctttatat gtagagttaa cgggaaatcg ttgaacttct
     6721 tgcgaaatat tgctataatg aacatgaaaa agaggagttg cgctaacaac ccctcattaa
     6781 cagcaccgat taaagaacgg tggcagaata ttagttattt ggaaataacc gctgacctgc
     6841 taaagtagac ggttatttct ttttgtcatt tttaagcata tttacaatca aactaatcaa
     6901 agcaatggta aacataccaa aaccaagaat ggtttgaatt gtctcatatg cggacaaaag
     6961 gcttactcct ttcatagatt ttagcactta cattcataag caccaccacc tttccagaag
     7021 tcgccaccgt cttcaaactt tactgtcttg actattatag catatctaaa atcgaataaa
     7081 aattctcaat tttagctgtt tttgctataa ttttagcgag gtgatcgtga tggaacaata
     7141 tattggttta ctcgataaat tagggtgatc aagacacagc cattgcttgt gcgatttaca
     7201 ttacgcacga gtcatgaacc aatcaattgt gtagtggcca atatagaaat agcggataaa
     7261 ttgctgattt tggaagaagg caagtatact atttcagtga caggtcactt caataaacga
     7321 aatcaactag tcattgaatc aatgcaaatt agaaaccctg atcattttac gagattaatg
     7381 ggcatataaa taaaagaaga gctgcagtcc taaatgagga ttgcagctct ttttgtttta
     7441 catagaaaca agaaaaatga aaaggccttt ttaggaaggc gcaagcgcct tctcaattga
     7501 aaaacctctc tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgttgc
     7561 acgttcacta agataagggg tcttgcaaag caagacaaaa tagaaaaaac ttcctctcac
     7621 caaaaggttc gttcctgttc gctatctctt tattgatttg ccagaccggc aacaagttgc
     7681 cttttatcac caaaaagcga caaggacaag ggtaaatggc acgctgcacg cccttgtcct
     7741 tgtcgctttt agttgattca ttctggccaa caaatcaata aagaaagaac taacgaaagg
     7801 aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc gaaagaaatt tgattattgg
     7861 ggtgttccct tcagcgaatt attcccaaac tatcatgcac catatactgt cgaatgcgat
     7921 tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt atcaatgtcc aacatgcggg
     7981 aaaaaatata ccttgtctta tggcgattat attttgattg atgagaaagg aaagaaacgc
     8041 tgatgaaaac acaacacaaa ttattgactg taaaagaagt cgttcaaatt ttggtggaca
     8101 aagaagtcat ttctgaaaaa gtagcagaag ctttacttat tccgcggtac tggacgatag
     8161 aaaccccttt agtagaggat gcaccgacta tgctatttga agacctagtg aagattgcag
     8221 gatatgaact gcattatcaa gaaaacatgg aaacttatga aaacgaatat tggttaacca
     8281 aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa ttactatgac gaatttgtca
     8341 aattaccttt ggataaaatg gcacaaaaaa tggaggatat gacattccta tacaatgaga
     8401 cacgtgttcc aaaaaaacat tataaggaaa gattgtcggt agctgtggaa gaaatgattg
     8461 aatcgggtgt cgaaatgaat ctcattgcga cctactatcg aacgcttgaa gaactgaaaa
     8521 agcaaaatgg acaatggttt ttccaagcct tactatgctt ggaagcaggc gtcaagccaa
     8581 acactatcaa accttctgaa taccaagctt tagaactaac gtatgggaaa tttgtcgaaa
     8641 cgaaaaaagc caagactgtt tcaagtgaat ggttggacta cttagaaaat gtcaacaagt
     8701 atggcgcatg ttacatcatg aaaaaggagg acaacgaaaa tgaataaggt gatttatcaa
     8761 ctaacaacgg aagatgtgtc acacttttta acaaacaaag aacatttgaa tgaagtaatc
     8821 agacggattc aaaaagactt tagttgtatg gaagaaatga agtactttat tcaaagagtc
     8881 gaagaacaat tgtatttgga aagtgaaacg agtcaaacga aggttacagt cagcaataat
     8941 tatttagacg tgtataaacg tcgtcctgac acattaaaaa aaggagcgga gcttctacga
     9001 ctagaaacag aaaaatatat catgaatgtt tacctagaaa ggagtgaact ggtcgaaaac
     9061 tttgtcagct acacgatttg caggaaacac tattactcag aatcagaata ttattcaatt
     9121 tttgagagca tggaattaat ggaagagcta aacagcaaag atgcgacggt agacgattat
     9181 taccttgtat ttactaaatt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg
     9241 gattatgaac tcgctgtacc cgaagtattt ttgagggaat atgtagaaga agtagataac
     9301 ccaggcgacg actggtacac gactttaact gattttttag atacctacac ttacgacgat
     9361 acagaagaaa tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg
     9421 aaaaaaggtc aataaccatt tataccgaaa agtgagaatg tgtgaaataa aaacaaaaaa
     9481 ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct
     9541 taggtggtgc gaaataagcg agcaaagccc gaaaacctaa gagaaagaag gagatttata
     9601 tgaacaaaat tattccctta ttattagtcc tatttgttgg agaaatactt atgcgactta
     9661 gtgcagctgt acatttaaac ctcgcagtat ttttggtaat actaatgttt tgtttgatct
     9721 tttggcgatt tgtattagcc ctcgtaagtc tactattcaa gatgattgca gccatatttt
     9781 tcataggatt atttcgatga aaggagagag taccaatgcc aattgcaata ggaaataaac
     9841 ggttacccgt gacgttggat gaaaagagac aaaaagaatt gcagtaacca aagcagaagt
     9901 acggcaaaag tgaatccagg attatgtgta ttgcgttaga tttattgatt gcgcaagaaa
     9961 aagcaggatt tgacatacca gcactcaaaa agtgatcgca ctttgcgcac gtgttaatac
    10021 gtgttataat tattttgtaa ggaggtatag tgcacaatgc cacttacggg aaaagaaatg
    10081 cttaagttat tgaaaaaaat ggctgggttg agcgaaggca agagggattt catcatcatc
    10141 tttataagga tggtgttaga atcactgttc ctgttcatgc caatcaagat cttggccgag
    10201 gacttgagcg aaaaatttta aaggatgcgg gattgaaata atctcgcatt cgattaaaga
    10261 aaggattgac tgaaatgtta agtgagcctt tagcaaaaac ctatcctgct atttttagcc
    10321 cagaagaagg tggtgggtac tttattgaat ttcctgatgt tcaaggtgct tacacaggaa
    10381 ttaacgaaga cgatatatct tatggaattg ctatggcaca agaggtgtta ggtatggttt
    10441 tagcagatta tattgagcat gaagatctac ttccagaacc aacaccaatc aataagattt
    10501 ctgttgaaga tgattctttt actactttaa ttcgtgtaga tgttgccaaa tatttgaaag
    10561 acacagaact tgtcaaaaaa accttaacaa ttcctcaatg ggcggataag ctaggcaaaa
    10621 gggctggcat aaatttttca gtccttctaa cagaatcaat tgcagataaa gcagataaca
    10681 ttcttcgttc tggaagaaat aattagaact caaaagggtc agaaacttaa aaatctttgg
    10741 cttctttttt tactacaaag atggcttctt taaattattt gcctgattga tttaaaaaaa
    10801 ttgcgcaaag actacgagct aattaagttg ctcgtggtct ttttttatga aaacttttta
    10861 gatc
//
LOCUS       DAXWLH010000086        10713 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXWLH010000086 DAXWLH010000000
VERSION     DAXWLH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.087"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(39..1301)
                     /locus_tag="ACGE36_001756"
     CDS             complement(39..1301)
                     /locus_tag="ACGE36_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384139.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1294..2193)
                     /locus_tag="ACGE36_001757"
     CDS             complement(1294..2193)
                     /locus_tag="ACGE36_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384140.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2338..4116)
                     /gene="uvrC"
                     /locus_tag="ACGE36_001758"
     CDS             complement(2338..4116)
                     /gene="uvrC"
                     /locus_tag="ACGE36_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFF8384141.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4298..4612)
                     /gene="trxA"
                     /locus_tag="ACGE36_001759"
     CDS             complement(4298..4612)
                     /gene="trxA"
                     /locus_tag="ACGE36_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFF8384142.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4699..7059)
                     /locus_tag="ACGE36_001760"
     CDS             complement(4699..7059)
                     /locus_tag="ACGE36_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFF8384143.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7174..7721)
                     /locus_tag="ACGE36_001761"
                     /pseudo
     CDS             complement(7174..7721)
                     /locus_tag="ACGE36_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7779..8216)
                     /gene="zapA"
                     /locus_tag="ACGE36_001762"
     CDS             complement(7779..8216)
                     /gene="zapA"
                     /locus_tag="ACGE36_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFF8384144.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8418..9335
                     /gene="rnhC"
                     /locus_tag="ACGE36_001763"
     CDS             8418..9335
                     /gene="rnhC"
                     /locus_tag="ACGE36_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFF8384145.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9591..10502)
                     /locus_tag="ACGE36_001764"
     CDS             complement(9591..10502)
                     /locus_tag="ACGE36_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8384146.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
BASE COUNT         3015 a         2297 c         1800 g         3601 t
ORIGIN      
        1 caataaatgt ccagcttcct tctatatttt tctatatttt atgctttttt tcgttcattt
       61 ctcataattg aaatcgattg tttcaatgaa ctccataatc cgccttcgct atacacgagt
      121 acattcgatt tgtacatgtt tgcagacaag atcatcaata agaatgttac ggctacaagt
      181 accaacaagg aaatcaatgc tccacccata ccgactgtct catttgctaa gcgaaccggc
      241 ataatatatg aagataagaa tggaatatac gaagtgatcc gaataacgat attgtttgga
      301 tctgaagctc ctagtatcaa tccaagcata tatccgccta gagatagata agtcactggc
      361 aaaattgctt ttgctgtatc ttctgctttg ttgaccaaag agccacataa agctgctaaa
      421 acagaaaaaa tcaaaatgcc aaagatcatg aatagaagag aataccacaa gaatggtcca
      481 atcaagttat ccaatgagat accgtctaat atatttttga ccatttccat atctttgaat
      541 tgactgtatc ctataccaaa aataataccg tataatgcca tttgcgttaa tgcaacaagt
      601 aaaacacctg ttagttttcc gtaaaaatgc ttttgtgcag tcgtactgga caggatcact
      661 tccatgatcc gcgtgccttt ttcagaagcg atctcttgtg caatgatctg cgcatacgtc
      721 aaaataataa tgaacaagac aatcgttgct acataactta cgacatattg gactgtcgag
      781 ttatcttctc caactgtcat ttttcctttt gaatcaaaat tgactttttg tcgagtcaaa
      841 cttgcttgtt ggctcaagct agctacttct tctggtgaaa tgcctaattg acttgctcga
      901 agcatcgatt gcaagccagt cagttgttgt tgtatcagta gttgtgtact ttgtcctaat
      961 gaattttcgc tataaagcat cccatttatc ttatcattct cagttgagac tactaaatac
     1021 gcatcgattt tttcttccga aagctctttt tgtgcttttt cttcagaatc aactgctttg
     1081 aactcaaaat catctgtttt gacttggctc atttgctgac tgatttgctc attatcagcc
     1141 aatacgccga ttttattgac atcactattt tgttgagcaa agtttcctgc tacataaatg
     1201 atacccagca tgataaaagg gactaatatc atgatcaaga aagaaatcga cttaacattt
     1261 tttaaataga catctttggt aataatccaa aacttacgca tcgatttcac ctgctttcaa
     1321 tttgaaaatc tcttccaaag ttggcggttg ctggttaaac ataggaatgt agccgaactg
     1381 agtcgcacga gtaaagattt ctttcccagc ttcaggatct tctaaggtaa tctctaacgt
     1441 attgtcttca tgaaggatca cttttttcac gccttcgatt tcttctactt cctgttggct
     1501 taaaccagat tccaagaata acttcgtacg gccaaatgat tcacgaatct catgtacttt
     1561 tccattcaaa accatttttc cgtttcgcaa catgatcaaa tgatcgcaga ttttttctac
     1621 attatccata ttgtggctag aaaaaatgac acatgaacca tgttttttca attccaatat
     1681 tccatctttt agcaattcag catttaccgg atctaacccg ctgaatggct catccaaaat
     1741 gatcaattta ggttcatgga ttaacgtggc gatcagctgg actttttgct gatttccttt
     1801 agacaaagat tttactttat ccgtcttttt cccttttacc tgaaattttt ccatccaaaa
     1861 atcgattttt ggttcaatct cttttcttga tttccctcta agttctgcaa aataaagcag
     1921 ttggtcttga atcgttactt tcggataaag tccccgttcc tccggaagat aaccgatgac
     1981 atcataatct ttctccccaa gatgatggcc attccataac acttctcctt ggtcttgtgt
     2041 caaaaaatcc aagatcaaac gaaaagtcgt tgtcttccca gccccgtttt ggccaattag
     2101 ccccatgatt tttccatcgg ggatttggaa agagacatga tcgacagctg tgtagttccc
     2161 aaaagtttta acaagatcct tgacttctaa catttcttca ctccttcttc aagaaatcac
     2221 atatatcctt agacaccaga cgtaagcgat ttattattta ccaacaaaac gtaaacgctc
     2281 tttttgttgt acataaaatg ataggacact ttactttctt tgtaaagtgc cctttcctca
     2341 ttcctgatgt aatttatcaa atacgttttg tgcgacattt ttcggcagtc cgatttcttg
     2401 taattcttct gtcgtggctg ctgtgatatt tttcaacgat ttgaactctt taagcagcat
     2461 tttttttcgt tttggtccta acccttcaat ttcatctaat cgggaagcaa aactgttttt
     2521 actgcgtagc tgccgatgga aagtgattgc gaatcgatgg acttcatctt ggattcgctg
     2581 gagtaagaaa aactctggtg aatttcgctc aagcggtact acagccaaat ctataccgaa
     2641 tagcaactca tttgttttat gtttatcgtt ttttgccatg ccagcaatag gaatatcgat
     2701 tcctaattga ttttctaaga catcttttgc cacatctact tgtccttttc ctccatcgat
     2761 caagatcaaa tcaggtaaag gcaacccttc gcgaatcact cgtgaatatc tgcgatagat
     2821 gacttctctc attgatgcat aatcatccgg tccgttgact gtttttattt tatatttccg
     2881 atattctttt cttgccggtt ttccatcaat gaatacgacc atcgcagcca caggattcgt
     2941 ccccatgata ttagagttat caaaagactc gatacgtatt ggggtcggga tattcatcgc
     3001 gtttcctaaa cgttcaactg ctccgatcgt ccgttcctgt tttctggcaa tcagatcaaa
     3061 ccgttcattc aaagcaactg aggcattctt attcgccaat tcaactaatt ttttcttttc
     3121 tcctcgtttt ggttgcagca ctttcgtagc taacaaagct tctacactgg gcttgtcgat
     3181 cgtatcagga atcaagactt ctttaggaat gaaatgctca ttttcctgat aaaactgacc
     3241 gaggtaagtc aagaaatctt cttctgcttc gttatagaaa ggaaacattg aaacatctcg
     3301 ttcaattagc tttccttgtc ttacgaaaaa cacttggaca cacatccatc ctttgtcaat
     3361 cgcataacca aatacatctc gatccagtaa atcagtattt gtcatttttt ggcgagtcat
     3421 gattgtctca atggcacgga tctggtctcg gtattctgca gctttttcaa actccatgtt
     3481 ttcagcagct ttttccattt tctgatggat ctctgactcg attttttcgt gtcctccgtt
     3541 taaaaacttt ttcacttcat caacgatttt cgtatagact ttcggatcta cgtcgaaata
     3601 ataaggacac agacattgtc ccaaatgata gtaaagacaa ggctccttcg tctgactagg
     3661 tttgcatttt ctcagaggaa ataatcgatc gaggatcttc tttgtctcat tcgccgcacc
     3721 gacatcggga tatggtccaa aatacagtgc tttgtctttt agtactttac gagtaatcac
     3781 caaacgggga tatttttcat tcgttatctt aaggaaagga taggttttat catcctttaa
     3841 cataatgttg tatttaggat catttttctt tatcagattg atctctaaga gcaatgcttc
     3901 gatattcgat tctgtaacga tgtattcaaa gtcttcgatt tcactaacaa gccgttccgt
     3961 tttcgtatta tggctgccag taaaatatga gcggacacga ttttttaata ttttggcttt
     4021 gccgacataa atgatcgttc catttttatc tttcatcaag taacagccgg gctgatcagg
     4081 aagtaaagct aatttgtttt tgattcgttc gttcatctgg tactcccctt catattcaat
     4141 taaagttatt atagcatgtc tgtcaaaaaa acaatccagc ctgacaacgt aattatactc
     4201 aaaaaatatt aatttgaata accttaactc aaccgcaaaa aaaccgagga cggaactgga
     4261 ttcagtccca ccctcagctt atttttgctg taagttttta taaatattga tcgatcattt
     4321 gacgtagttg ttcttttgaa tgaacaccaa cagctttttc tactacttga ccatcttttt
     4381 tcaacattaa tgttggaatg ctcatgatac cgaattgttg aggagtttct ggattttcat
     4441 ccacatccat tttagcaatt ctgaattctt cttcatcata ttcttgttcc aactgatcca
     4501 aaatcggtgc ttgcatacga catggaccac accaagttgc ccaaaaatca atcaatacaa
     4561 gtcctttatc tgtttcttca ttaaatgtct tatctgtaat tacttgtgac atatcaaaga
     4621 cctccatttt taaaatctat gattagtata gcatttctat acttggatag cgaaccatct
     4681 gcttaccttt agtaagcttt acttaaattt aacaattgtc gcaccatttc cgccttggtt
     4741 tgccggtgca aactcaaagc ttttgacgct ccgatggttt ttaagataat cagtaattcc
     4801 cttgcgaagt gcgcctgttc cttttccgtg gacaatagtc acttgaggat aaccagctag
     4861 aatggcagaa tccaaatatt gatctacctc acttagcgct tcttcgtatc gttttcctcg
     4921 aaggtccaat tgattcggca cgtgactact agattcagat cgtacagcat ggataactcg
     4981 ttgtgtcggc tctttttgag gagcaacagg cgtcagctcg tcttcggaca cattcatttt
     5041 taatataccg atttctactt gccactgatt cccgttttta cgtaaaagcg tccctctttg
     5101 gccgtaagtc gtaaccaaca cttcgtctcc cggtttcaac gttttttgtt ctttcgcctt
     5161 cttcaatact ttatttttct tcaaagtttc ttcttgatgt agatttgcta attgggtctt
     5221 tgcatcgatc aactgatgct ctttcacatt tccttgccca atctgctgtt gcattttacg
     5281 aatatcagca atgatttttt ctgctttttc ttccgcctct gacacgactt cgtttgcttt
     5341 tttctttgct ttttccattt ctttttcacg ttcttcaaag aaatagctgt aagcttcttt
     5401 caaatcatcg tgaagttctt gtgcctcaga aacaaaatga cgcatttcca gatattcggt
     5461 ctctgccatt ttccggcgat tctctaaatc tgtgatcatt tcgtttaaat cttggctctc
     5521 atcattcatc aactgcttcg cttcatcaat aacttctgta tcaagaccta atcggctaga
     5581 gatttcaaac gcattgcttc gtccaggaac gccaatcagc aaacgatacg ttggacttag
     5641 tgtatccaca tcgaactcca tgctggcatt aatcgtattt gcccgattgt atccataaac
     5701 tttcaattca ggataatgcg ttgttgccat cacataagca gactttttac caagatcatc
     5761 taagattgcg attgctagag cagcaccttc ttgcggatct gttcctgcac cgagctcgtc
     5821 aaataatacc agactttttt cattgacttt tgataaaata tctacagtgt tcgtcatatg
     5881 agaagaaaag gtcgatagac tttgctcaat cgactgttcg tctcctatat ccgcaaatac
     5941 ttcctcaaaa ataccgattt gactttcttc acctgcagga atcggtaatc ctgcttgccc
     6001 catcagttgt aacagaccta aagtcttcaa cgtgatcgtt ttcccacctg tgttaggtcc
     6061 tgtaatgacg atggcttgat agtcttttcc gattgtgatg tcattagaaa ctactttttc
     6121 ctgatcgatc agtggatgtc ttgcttgttt caagacgaca tgattgtctt cactgattcc
     6181 cggcacgatg gcttttactt ctttaccaaa tcgtgctttt gcattcatca gatccatttt
     6241 accaatcaca taagcattgt ggatgatttc ttgacgatgg ggaacaagtt cagcagaaag
     6301 ttcacttaaa atacgttgaa tctcatttcg ttcagcgatt tggtattgac gcaaacgatt
     6361 attcaaatcc accacttgtt ttggttcaat aaataaagtc tgtcctgaag cactttgatc
     6421 gtggactaca ccgccaaata caccgcggta ttcttgtttg actgggataa cgtagcggtc
     6481 atttcgcatg gtgatgatcg catcacttaa atactttgca tttttcccac ggacgatccc
     6541 atctaactgt tcacgaacag cttgttcact tcgccgaatg ttctgtctta tgctctttaa
     6601 ctcaggagaa gcgtcatcag tcacgcgtcc gtcttcatcg attgcttcct ttaaacggcg
     6661 gctcaaaaca gggatcgtga ctaactgatc tgcccacata tacaaacgtg caaattcgat
     6721 ttcactgtca gaaagatcat caatgaaacg attcaattcg cttgtcgtag acaatactct
     6781 ggaaacttgt gcaagctcca ctccattcaa gtctgctccg atctcgatcc gtttcatatg
     6841 gggacggata ttttcaagtt taggtatagg gatcccgcct cgcagacgtt gcactttcaa
     6901 tccatcctca gtttcttgaa gccagtttgt gatcgtttgt ttctcactca ctggtaccaa
     6961 ttctgccagt tcttcttttc cttgtgcagt cactacaaat tgtctgacca tctgtttgac
     7021 tttttcaaat tccaatgttt caaggattcg gttattcatt ttatcacctt atctttcact
     7081 atgaaaaaag agctgtgccg gacatacgct acgcagcctt cttaaataaa ctactctgta
     7141 caaaaaacga aatattttcc agcactattt tccttaattc aaaatattcg ttaccaaagc
     7201 tcatacatct tgttggtaag aaatggcgtt ctctcaacga taaaacgagc gaaactatgc
     7261 tcgccgaatt gattttggac cacatctaat ggaacaaagg caagtaactg caaaatcaaa
     7321 aatatagtaa cgtaagccat gattagtgac aggatgcctc ctgcaatcca gtctacctgt
     7381 ctcaagatcg gcacatacgt caatccatgg acaaaaaccc ccacaaatcg ggtaagcagc
     7441 cagccaatta gcaaaataat cagaaaagca gttcctgcat aaaaagcttc atctaggtga
     7501 aaagcaaata cctgatcaaa aaatgctaac tttgatgttg gggtgacagc tgggtatgga
     7561 atgtataatt ccaaatgact tgctaatggt ttgtaaaaat gctgggcagc aataaatgac
     7621 aatacatatc caatcgcgaa tatgatttgc aaagcaaacc cgcgacgtgc tccagaaaaa
     7681 aacgcaatta gcaataaaaa taaaatcagc aaactcagca tgctacgttc ctttctcttc
     7741 taactttttt aaatttttct tattttgata aaaggatctt aattagaaga tttttgttta
     7801 atttgtcctt ttcgtttctc gtttaaaatc tgttgtgctt ccacatgatt tttaatggta
     7861 tgatctgctt gaccattttc tttcaatact tcccgagctt cgttctcgat tgcttcaatg
     7921 cgtttcacac gattttcaag ctctgtaaat ttgatcattt ttttctttaa ttctgctgtt
     7981 tcttcttcca gctccaagat tctttcttgt ttcttcaatt gatccgaaag cgcattgaca
     8041 gccatcaaga ttgctgcttg ttcattatcc atttgtggtg ataattgttt caattctgct
     8101 aactgttcat tgatcagctt cgttacgata tccatatgat gcttggtttc ccgtccgata
     8161 atagtatatg tttggttggc aatcactgct ttgtagcgtg ttttttcgtg cgccatacga
     8221 atcatttcct cctatttgat gaaagtttta tttagtgtac catgaagttg gttctttcac
     8281 cgttctccac aaatattttt cacatactta actattatat gataaacttt aaaaaaatgc
     8341 tatgtctcgc aatctcttag aaaacgaaaa gtcgtgagtt gcggtatagt tttatgtata
     8401 aaaacgagga gaattttatg agcaacgtca ttatcaaagc aacaaacgac caacttagtc
     8461 aaatgaaagc atactatcgc tcttatttgt taaataaaca gattccttat actagctttg
     8521 ctgcaaaaaa agacgggacg accattaccg cttatacatc cggaaaagtg atgtttcaag
     8581 gtacgaatgc agcaatagaa gctgataaat ggggggaggc acaggataaa caagccaagc
     8641 cagcaaaaac agcctcttct ttgccaaaaa atatcagtca attatccgtt ttaggtagtg
     8701 atgaggtcgg aaatggcagc tattttggtc cgctaacagt atgtgctgct tatgtgaaac
     8761 gtgatcagct tgcagaatta cgtaaacttg gtgttagaga ttcaaaagag ttaaaagata
     8821 gccaaatcat ccagcttgct gacatcttaa aaaaaaccat cccctataaa ctgcttgtct
     8881 tatctccaga aaaatacaat gagatccaac caaaatacaa tgccgttcgg atgaaagtcg
     8941 ctttgcacaa tcaagcgatc catttgctgc taaaaaaaat cgagccagaa aaaccagaag
     9001 cgattttgat cgatcagttt acgccggaag caaattttaa aaaatatgcg cgtttagaaa
     9061 aaaatcaatt acaagaaaaa ctgtacttcg ttactaaagg agaacaatat catttagctg
     9121 ttgctgctgc ttctatcatc agtcgtgcca gctttttgga agagttacac aaagcatcag
     9181 ctgaagctgg aatcaccctt ccatcaggag ctggaacgaa atctgaccaa gtagcggcaa
     9241 aactattgga gaaaggcggc atgcctatgc ttgaaaaata tgccaaactt cattttgcca
     9301 atacagaaaa agctttgaag ctgctaaaaa aataatggaa ctgtgacaaa agtcatgtca
     9361 cagtcccttg ttccgaataa acggtgttct catgccaact cctcggtcac aagtcacaat
     9421 agtcatcaaa catgagaagc tgttttttga aattgctcct tgtgacgcgg agttaaacgg
     9481 caggctcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcga taataagccc
     9541 ggccaaacga aaaatatgag gagctatttt tgtttggctg ggcttattta ttactgaaaa
     9601 taagtggtca atgcttgtgt caaacttgca gcaatctgct gctgataact gtctgttact
     9661 aattccttta aatcactatc attattcata taacccatct cgattaaaat agaaggttga
     9721 agattttctc gaagaacttg atagttccca gtggagattc ctcttgaatc cagaggcaga
     9781 tcagtgaagg agtcagcaat tgtctgtgct aatgtttcct cttgtccata gtaataatat
     9841 gtcgtgattc cagttgcttc attcgcatat tccgtagagt ctgcgtgtaa actgataaag
     9901 acatctgcct ttttttcttc actgtagtcg catatatctc caagctgtac taattcatct
     9961 gtagtatggg tcaaataaac cgttgctcca gcatcctcta gcgccttttt tacttttatg
    10021 gctgtactta atgttatatc tttttcttct gtttgttcat cattgcttaa tgccccagta
    10081 tcatttccgc catgcccagg atctaataca atcactgcat cagatagctc ttccggtttt
    10141 gtttgtatat tctttgtgac tttggttcca tcttcgaggg ctaaatatcc agtatagcca
    10201 tcatttgtct tgatctgata agcatttttt gtttcagaaa gaaactccgc cagctctccg
    10261 ccgtttatag ttgcaagtgg ggtagttagc gagtcatctg tatatagtgt acggtcattt
    10321 tttccgattt tgattaatgt ctcttgttct gtatcatcaa atgattcctt tgtctttgta
    10381 accgtactgt tcgtactgct gaccgtttgt ccaactatct ttttttgacc aaaaacatct
    10441 atttgaaaac agataataat gataatgatt gcaaatacaa aagctccaat tatttttttc
    10501 attcttgact ctcacctcgt agatccagac taagggtcaa aacttaaaat cagcttaagt
    10561 acgcaaaaaa ggggctgtaa aagatataac cgtagaaaat aacattactg ctaaaatgat
    10621 ttttttcatt tttggcatat cctgaaatct ccttttgact attcgtggtg attcatctac
    10681 ccaataaata gtttccttca ttctacaaac aat
//
LOCUS       DAXWLH010000087        10710 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXWLH010000087 DAXWLH010000000
VERSION     DAXWLH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.088"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(3..1304)
                     /locus_tag="ACGE36_001765"
     CDS             complement(3..1304)
                     /locus_tag="ACGE36_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8384147.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(1534..3525)
                     /locus_tag="ACGE36_001766"
     CDS             complement(1534..3525)
                     /locus_tag="ACGE36_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFF8384148.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(3669..3935)
                     /locus_tag="ACGE36_001767"
     CDS             complement(3669..3935)
                     /locus_tag="ACGE36_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8384149.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE36_001768"
     CDS             complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE36_001768"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFF8384150.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE36_001769"
     CDS             complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE36_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFF8384151.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            7279..7842
                     /gene="def"
                     /locus_tag="ACGE36_001770"
     CDS             7279..7842
                     /gene="def"
                     /locus_tag="ACGE36_001770"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8384152.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(8095..8616)
                     /locus_tag="ACGE36_001771"
     CDS             complement(8095..8616)
                     /locus_tag="ACGE36_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFF8384153.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(8619..9461)
                     /locus_tag="ACGE36_001772"
     CDS             complement(8619..9461)
                     /locus_tag="ACGE36_001772"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFF8384154.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(9454..10179)
                     /locus_tag="ACGE36_001773"
     CDS             complement(9454..10179)
                     /locus_tag="ACGE36_001773"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFF8384155.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            10355..10651
                     /locus_tag="ACGE36_001774"
     CDS             10355..10651
                     /locus_tag="ACGE36_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384156.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         2994 a         2308 c         1966 g         3442 t
ORIGIN      
        1 ttttattcta tttttctttc cgtggactct actttgaaat aatcgatatg tgcatgctgg
       61 tattctaata ccgtgaaacg gaatgatgca atctcaatga ttgttcctgc ttttatttct
      121 ttatatcggc tagaagcaaa gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga
      181 atctctacat gaaaataacg ctggaaatca tctaatgtta gtttgccgga gatgatataa
      241 gagccatctg actgttccgc tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc
      301 ccgacgattt cttctaaaat atcttcaatc gtcactaagc cgcttgtccc tccgtattca
      361 tcttttaaga tagcaatatg ctttcccttt tttcgcatga tcgtcaataa tttttttata
      421 gggatcgttt cagttgttac gatcggctct tcaatcaact gttctactgt cagctggtca
      481 tctttataag attgcttgat caaatctggc aaggtgacat aacctaaaac attgtctttt
      541 gattctaaga taaccggata gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct
      601 ttgacggttt ctgaagcttc gaatacttgc atggacgtac ggtctacttg tatatctttt
      661 gccaaggtat catcaaattc aaacacgttt tccaaataat gatattcttc tttggtcaac
      721 tctccttttt cataagaatt caatgcgact aatgtcagct cttcttgaga caatgtctct
      781 tctccttcag atacgagacg cacacctaat agcttcccta gccctgcagc tgaatgattc
      841 aatacccaaa taaaaggaaa cattacttta taaaaataat gaagtggttt aactacgaac
      901 aagacgacct tctccgtatt gacgatacta tagctcttag gaagcagttc gccaataacc
      961 acatcgatat acgtaactaa aataaatccc agcgccacag agattgtact gctaatagcc
     1021 gaactaaaag gtagtttacc gatcactggt tctagtaaag ccgcgacaga tccttcaccg
     1081 acccacccga tgatcaaacc ggttagcgta attcctaatt gactagctga taaatagtta
     1141 tctaaatggt gtgtaacatg aagagctagc ttggcgttct ttacattttc cttttctagt
     1201 tgttccaatc ttgatcgtct gattttcact aaagcaaatt cacttgctac aaaaaatgaa
     1261 gtaattgcaa tcatcaaaaa taaaataatg aaattgataa ccatattctc ccgcctttct
     1321 ctttttctat tgtacccttt ttctacaaaa agattgaaaa taatgcgtat tcatatttag
     1381 gaataaaaaa ggagatgcgg catttttcag ccacatctcc ttatcacttc aaaatcattg
     1441 cgttacttgt tgctaaacag ctttttcatg acctctgata aacggtgttc aagtagctga
     1501 cattcgacat tagttcaggt caaagcgcta ctttcacaac cgctctttca accatgtttt
     1561 ccgagtattt ccaatgataa gcggaacagt ttcatgtttt acttcgctgt actctagccc
     1621 aaaccatgtt tctggcgagg tagcgatttt tttgattgcc agttttgcct gcatgatttg
     1681 acgttgccat tttcctagag ttgtcacatt ggaaactact tcatcaatca ggacaaattg
     1741 gaaatcgcca acttctcgcc ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg
     1801 gccttgtttc atcaaatcat ggatgacttg gcgaacatat acgttgattt cctgttggac
     1861 cctgaaacct aagaacagat tcacttggac aataaaatca gtgtccatca tatctacttg
     1921 atattctttc gtatatggct catccgtcac ttccacatga acaaaccaat agactcgtgc
     1981 tcttttaggc cgtttatcta aaatcgaata gatgaactgt cggccaactt tatcttcagc
     2041 catatcagga accaagaaga ccacatttgt ctgatagatt ggtaaagaag tatcatcacg
     2101 taattctcta agctgcggga tataatctct caaagacaca tcctcggttg acgattcgcg
     2161 aataatgttc ccttgttccc aaataaacat cacgactaag atgatcaaac caatcagaac
     2221 ggctacaaaa ccgcctcgga agaatttgac aacacttgag ataaagaaaa cgacttctat
     2281 cgcaccgaag aaaaaggtaa ctaaatatgc aagccaacga gaatgcccat tttggataag
     2341 ataaaagtaa agcagtgctg ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc
     2401 agcttccata tgatgagacg ttcggaatgt gacaaccact agagaacagg cgatccataa
     2461 aatcagattg actgctggga tatacatttg cccgatactg ttcccaggat acatgatctt
     2521 taaccgaggc agcagtttca acttgattgc ttctgaaact agcgtaaacg aacctgtaat
     2581 caatgcttgc gaagcaatga ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca
     2641 actttgaggc atcatttgga agaatggatt caggttcttg atttgctgtg cttcagggtt
     2701 ttgataaaca ttcaacaacc acgcagcctg cccaaaataa ttgataacca aacaaatttt
     2761 gacgtaaggc cagctcagac gaatatttcg tttgccgaca tgtccaagat cggaataaag
     2821 cgcctctgca cctgtcgtag ccaggaaaat atttccaaga atgaacaagc ctaatttgtt
     2881 atctgcacta aaaagcagct ggatagcata ataaggattc agtgcacgga taactgtcca
     2941 gtcttgactg aagttcatta atcccattac gcctaaaaaa gtaaaccaag cgaacatgat
     3001 tggtccgaat gcttttccga caatatctgt accaaagcgt tggaccatga ataaaacaaa
     3061 aataataact aaagtgataa tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa
     3121 ctgcggaatt cctcgcaagc cttcaatcgc agtcgttacc gtcacagcag gggtcaatac
     3181 cccatctgct agtaaggcag ctcctccgat cattgcagga atgatcaaat atttcccacc
     3241 ttttcggaca agtgtgtaga gtgagaaaat tccaccttca ccatggttat ctgcattcaa
     3301 tgcaatcacg acatatttga ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac
     3361 tgatccgata acaaaatcag gattgattcc tgacaatccg ccattatctt caacaatcga
     3421 tttcataacg taaagcggac ttgtcccgat gtctccgtag acgactccca tcgctacaag
     3481 caggccagcc attgatatct ttttggtatt gtgcgttgtt gccacgtttt tccctcattt
     3541 cttgttatat ggatatcctt taggtaagct tgaatgatag atgatattct tatccatgac
     3601 acccgctttc ctaaattgct tctaattatc caagtaacac actgaagtgt caacttattt
     3661 gaataaaatt attctttctt tggacgttct ctgatacgtc ttgccacaaa tgaataaaga
     3721 gcaaggcaaa aaattgcacc caataatgct ggtatcaaat aaaaaccgcc gagttccggt
     3781 cccagtggtc ctagtaaaaa ttcgccgagc caactaccta aaaaaccgat aatgatattt
     3841 ccagatacgc ctcccgggac ttcctttcct aaaattgcgc cagctatcaa acctaatata
     3901 ccgccgataa ataacgacca taaaaaattc agcatctcct ttccctcctt tatccatatt
     3961 tctcactttt attttacgct ttattcagaa ggactccaaa aatatgcgcc aagaaactcc
     4021 agaaaaaaag aagttgtaac aaaagtgttc agcttatttc cgtagaagct gcttctggaa
     4081 caccgtgaat cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
     4141 aaaaatagtt cctcatattt tgccgttttt tgagctaata tcgagggaca gctgattgaa
     4201 catcgtttat tggagaggtt gtaaaaaagc tgttctgcat caagtaataa gaacagccaa
     4261 gtaaaaacag ctcctaatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt
     4321 tgaacaccgt ttattcgaaa aaaaaggact gtgacacggc ttttgtcaca gtcccctttt
     4381 ttcgaataat tattgtcccc ctgtcacctt cacgatcaca agtacattat tcctatgaat
     4441 taaattattt ttcttcattt ttcgttgaac catcttcgtt caacattgct tttcttgaca
     4501 gatttacgcg tccttgttta tcgatttcag ttactttcac aaggatctca tcacctagtt
     4561 taacaacatc ttcgacttta ttgacacgat cgtttgctaa ttgggaaata tggacaagtc
     4621 catcttttcc tttgatcaag ttgacgaatg caccgaattt ttcgatacgg acaactttcc
     4681 ctagataaac ttcaccaact ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt
     4741 ttttgatcat ttctgcatca gctgaagcaa tacttacttt tccatcttga tcgatatcga
     4801 ttttcacacc agtttcatcg atgattccgt tgatcgtatc gccgccttta ccgataacat
     4861 ctttgatttt agcaggttcg atctggatca tttcgatttt tggtgcatat tggcttaatt
     4921 cttcacgtgg agcagcaatc gttgaagtca attcttctaa gatttccata cgtgcttgtt
     4981 ttgcttgagt taatgcttca gttaggattt gttctgtgat tccttgaatc ttgatatcca
     5041 tctgtaaagc agtgatacca tcttttgtac cggcaacttt aaagtccata tcgccaagat
     5101 gatcttccag cccttggata tcagttagga tcgtatagtt ttcaccatca gaaacaagtc
     5161 ccatcgcaat accggcgact ggcgctttga ttggtacacc tgcatccatc aatgctaatg
     5221 tgccagcaca gatacttgct tgggaagaag aaccatttga ttccaatact tctgctacta
     5281 aacggatcgt ataaggaaat tcttcttctg aagggattac ttgtgctaac gcacgttcac
     5341 ctaatgcgcc atgtccgatt tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa
     5401 cagaaaattg agggaaattg tagtggtgga tgaatcgttt gctgacttcc acccctaaac
     5461 catcaatgat ttggtgctct cctagaggtg cgagtgtaca agctgataaa gcttgtgttt
     5521 gtcctcgtgt gaacaaacct gatccatgta cacgtggcaa taagctgacc tctgatgaca
     5581 atggacggat ttcatccaat ttacgcccgt caggacggat cttatcgatg gtgatcaatt
     5641 cacgtacgac atctttttca agatcttctg tgatttgttt aacttctttc aacaagtgat
     5701 cttcatcttc atgacctgca aatttctcag cataagcttc gcggatatcg atcttcacct
     5761 gctcgatatt atcttcgcga gcaagttttt cttcagtcat tactgctgct ttcattgtag
     5821 cgtaagaagc atcaaagatt tcttttttca atacttcatc aacttgcagt aaagtcactt
     5881 ccattttttc tttccctact gcttgaacga tttcttcttg gaaagcaact aactctttga
     5941 tagcgtcaaa accaaacagc aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc
     6001 cgctttcaac catattgatg gcttgtttcg ttccggcaac agtcaattcg atatctgttt
     6061 gttctgcttg ttctactgtt ggattcaaga cgtactcgcc atttacacga ccaacttcta
     6121 cgcctgcgat tgggccgtca aatgggatat ctgagattga tagtgctaaa gaagaaccta
     6181 gcattgctgc catagctggc gagcagtctg tttctacact catcacgatg ttcgttactt
     6241 ggacttcatt acggaagcct tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg
     6301 cagtcaatgt tgcttccgta cttggacggc cttctcgttt gatgaaacct ccagggattt
     6361 tccctgctgc atacattttt tcttcataat tgatcgttaa tgggaaaaag tctgtatctt
     6421 ttgcttcttt agaggccaca gctgcactta agactactgt atctccataa cggactaaaa
     6481 cggctccgtt tgcttgtttt gctagctgac cgacttcgat ttgtaaggga cggccgcccc
     6541 atgtcgtttt gaatacttgt ttttctgaca taacttctcc ttttccttct cgtgaaagca
     6601 agacgctact aaacaaatga aaactgccga aagccgtctt ttgagcagct ttcatttggt
     6661 tagtaggagt ccatctagct tgatcacgga attttactta attgatatac gagtaaaaaa
     6721 agtgagattc atgtgaatcc cactcttttt gactaaaatt aacgacgcaa gcctaaacgt
     6781 ttgatcaatt cgcggtaacg ttgaacgtca gttttacgta agtaagctaa caagttacgg
     6841 cggtgaccaa cttttttcat taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt
     6901 gcatgttcat tcaagtggtt gatttcttca gttaatacag cgatttgtac ttctggagaa
     6961 ccagtatccc cttcatggcg agcgtattcg ttgatgattt cattcttgcg ttcttttgaa
     7021 attgccattt ctttcacctc tttcgattat tcccctgcga ctgagtaaaa cgttggtgat
     7081 tcgcttaacc aagtggcagg tcatgtgcct agagcacaca gctatacttt acctgaaacc
     7141 gggttgaaaa tcaagtagtt aatcattaag aaaaggtagt tttcaaacaa tcttactact
     7201 acttttccat ttttgtggta aactataatg cgaaaataga cttgaaacac agtcttttaa
     7261 gaaaggggta catattccat gattacaatg gatgatatca ttcgtgaagg aaatccgacc
     7321 ttacgcgaag ttgcaaaaga agtttctctt ccattaagcg aagaagatat ctctttagga
     7381 aaagagatgc ttgagttctt aaaaaacagc caagatccaa tcaaagctga agaactacac
     7441 ttacgaggcg gtgttggctt agctgctcct caattagata tttctaaaag aatcattgct
     7501 gttcacgtac caagtcctga cccagaggca gatggacctt ctctaagtac tgttatgtat
     7561 aatcctaaaa ttttgagcca ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt
     7621 ttatctgttg atcgagaagt accaggatat gttgttcgcc atgccaaaat cactgtgtct
     7681 tattatgata tgaatggtga aaaacacaag attcgtttga agaattatga atcgatcgtc
     7741 gttcaacatg aaatcgacca tatcaacggt gtgatgttct atgaccacat caacgatcaa
     7801 aatccatttg cactaaaaga aggcgtattg gtgatcgagt agagaattgg caggttcctt
     7861 cgatactatg ctacaagaaa gctttataaa aaaggggagt gctcaaaagc tgcacttccc
     7921 ttttttatgt ttctccacta atcgaaattt agatagacat ctagaaatcg caattccaaa
     7981 aaattctgtg tattggatac gcatcttttc ttattttttc ttatttcttc catatgggtg
     8041 tttatctcag agatgataga atagtttcta ggataaaaag tagcagctac gatgttatcc
     8101 ttcgtagagg attcctgctt cagctagttt ttgtttgatg tttttcagca tatgcttttc
     8161 tgggtagcgg atcgtatgca gatggatgcc gcctgttaat tcggataaca aagtagcatt
     8221 acttttttga taacgtctca taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt
     8281 tcctgctaac tcaccgtaaa ggggatgttc cacaattaca tcaatgattt caccaccatt
     8341 ttcaacgatt agtctcagtt cttcttctgt ttgatctttt ccatgctgga cagcgatttt
     8401 actgatgtgc ccagtattgg tttcttctgt ttcgaggata taacctcttg ctgttgccac
     8461 gatactttta cctgcagctc gtagtaaagc aatatcgcct acaatgatct gacggctgac
     8521 tccaaaagtt ttcgcaaact tgctagcact cactggtttt tctgtatttg ccaaggtttg
     8581 catgatttgt tttcgccgtg tctctccttc gatcacactt attcctcctt cattttgttt
     8641 tggtattcgc gcgagtgttt catcaaatca acatacgtat ctgttattgt ttccttatat
     8701 cccttatttt tcagcagact aacaacttta tccaatactt gctgctctct ttttgcatca
     8761 taaactgctt ggttttcttc ttttttcact tgtcctattt tagttaccgt atccattctt
     8821 ttttctaata aagcaaccaa ctctcgatcg atcatatcga tcttatgacg taatgtttca
     8881 agagactctt ctgttagttc ttcctctact gccttgatga tacgagcagg atttcctgca
     8941 atcactacat tgtcgggaaa agatttggtc acaaccgaac ctgcgccgac tactacgttg
     9001 tcccctagag tgacacccgg cgtgacgata actcctccgc ctaaccaaac attattaccg
     9061 attttaatgg gttttgcgta ctccagaccg ctgttacgtt tcactggatg tagtggatgc
     9121 gtcgccgtgt aaagctggac attcggagcg aacatgcagt tatcaccgat ttcaatcgtg
     9181 ctgacatcta aaaatgtaca attgaaattt gcataaaaat tctccccaac aaatatatta
     9241 tatccgtaat caaaattgat aactggctcc atatagattt ttttccctgt acggccaaaa
     9301 gtctctttca acaattggga tcgcagttcc gctgattctg cttggttgat gatttggctt
     9361 tgacttcttg caaacatacg gtcggcaacc agctcaggat cattggcaaa ataaagctcg
     9421 cctgcaatca ttttttcttt ttcggttttg aattcagtca taatctgctc ctaattcttg
     9481 tatttttttg aaaacaaccg ctccgtcttc ttgaataaac gtataaggct ccgctaaata
     9541 aacaggcgtt tgattgatta ctaagatttt ggctgacggc tggcgaaact gtaccagatc
     9601 acaaaacgga tgaacttgaa aactcgttcc aacaatgacg atcagctctg cttggctgac
     9661 ggcataagcc gcctgctcca tgactccatc ttgaaaccct tcaccatata acacgatgtc
     9721 tgggcgtatt tgtcctccgc atgcttgatg tttgtcgctg tgaagatatt ctttccagtc
     9781 taccgtttgt tgacattttc gacaatgaca ttgatacaaa cttccatgaa aattgacgag
     9841 ttgcgggctt ttagcctttg cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt
     9901 tttttctttc gctagatgtg ccatttccaa atgaatgata ttgggttttg cttcaggatg
     9961 ataaatcttt ttgacaaatt gatagaattt atccggttca ttcaccattg cttgatgact
    10021 cagcaagtat tccggctgct ccatcccgtg gtagattcct gacaaagatc gatagtctgg
    10081 cactccagaa ggtgtggata cccctgcacc ggtcaaaaat gtaatttgct gataagctaa
    10141 tatctctttt gctgcttcgt ctactgttaa ttgtttcatg ttttgtcacc tcactttcca
    10201 ttatagccca tactcgaaca aatttattgt tgaaaagtca gaaaaatggg aataaaaaag
    10261 tgttatgggc atttgaacaa tttatcacaa tgttcactga caaaagaatg aaaacgtgtt
    10321 acaatactgc tgtataaaag tgaggtgaat cgatatgtta ccattcttta tttattgggg
    10381 cgttattctg gcttgtatcg cctggttagc gttaagcgtt tatttctctg ttttttatct
    10441 tgtacgcaaa gaaaatggaa atctttgggc ttttgcttta ttcaacgtga ttgccgcaat
    10501 tgttttagcc attacattag ccgtatatcg aacatggggc tggggaatca cccaatactc
    10561 aagtttgatt tatctaatat tagccattta tggcgtcgtt gttcttttgc aagcgatcct
    10621 aggacgtgag ccaaagaaag cggcagctta aatgatcgag atggaactgg acacaggaaa
    10681 ttttctgttc tagttctttt tttattgtat
//
LOCUS       DAXWLH010000088        10454 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXWLH010000088 DAXWLH010000000
VERSION     DAXWLH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.089"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(78..2198)
                     /locus_tag="ACGE36_001775"
     CDS             complement(78..2198)
                     /locus_tag="ACGE36_001775"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8384157.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACGE36_001776"
     CDS             2428..3606
                     /locus_tag="ACGE36_001776"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFF8384158.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE36_001777"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE36_001777"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFF8384159.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE36_001778"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE36_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFF8384160.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACGE36_001779"
     CDS             complement(4959..6212)
                     /locus_tag="ACGE36_001779"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8384161.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE36_001780"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE36_001780"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFF8384162.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACGE36_001781"
     CDS             7789..9135
                     /locus_tag="ACGE36_001781"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFF8384163.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACGE36_001782"
     CDS             complement(9443..9829)
                     /locus_tag="ACGE36_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8384164.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACGE36_001783"
     CDS             complement(10097..10246)
                     /locus_tag="ACGE36_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384165.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3332 a         1886 c         2104 g         3132 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaa
//
LOCUS       DAXWLH010000089        10353 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXWLH010000089 DAXWLH010000000
VERSION     DAXWLH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.090"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            6..305
                     /locus_tag="ACGE36_001784"
     CDS             6..305
                     /locus_tag="ACGE36_001784"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8384166.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACGE36_001785"
     CDS             378..1739
                     /locus_tag="ACGE36_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8384167.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACGE36_001786"
     CDS             1761..2021
                     /locus_tag="ACGE36_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384168.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACGE36_001787"
     CDS             2035..2787
                     /locus_tag="ACGE36_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFF8384169.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACGE36_001788"
     CDS             complement(2881..3486)
                     /locus_tag="ACGE36_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8384170.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACGE36_001789"
     CDS             complement(3539..4513)
                     /locus_tag="ACGE36_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFF8384171.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACGE36_001790"
     CDS             complement(4891..5610)
                     /locus_tag="ACGE36_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8384172.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGE36_001791"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGE36_001791"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8384173.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACGE36_001792"
     CDS             7161..8558
                     /locus_tag="ACGE36_001792"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8384174.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACGE36_001793"
     CDS             8639..9079
                     /locus_tag="ACGE36_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8384175.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACGE36_001794"
     CDS             9227..9553
                     /locus_tag="ACGE36_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8384176.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACGE36_001795"
     CDS             9554..10303
                     /locus_tag="ACGE36_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFF8384177.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3285 a         1825 c         2146 g         3097 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aag
//
LOCUS       DAXWLH010000090        10246 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXWLH010000090 DAXWLH010000000
VERSION     DAXWLH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.091"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            151..300
                     /locus_tag="ACGE36_001796"
     CDS             151..300
                     /locus_tag="ACGE36_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFF8384178.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            311..1825
                     /gene="dltA"
                     /locus_tag="ACGE36_001797"
     CDS             311..1825
                     /gene="dltA"
                     /locus_tag="ACGE36_001797"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFF8384179.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE36_001798"
     CDS             1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE36_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFF8384180.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKYIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKLFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE36_001799"
     CDS             3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE36_001799"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFF8384181.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE36_001800"
     CDS             3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE36_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFF8384182.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4717..5799
                     /locus_tag="ACGE36_001801"
     CDS             4717..5799
                     /locus_tag="ACGE36_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8384183.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5799..6602
                     /locus_tag="ACGE36_001802"
     CDS             5799..6602
                     /locus_tag="ACGE36_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8384184.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6672..7412
                     /locus_tag="ACGE36_001803"
     CDS             6672..7412
                     /locus_tag="ACGE36_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8384185.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7423..8427
                     /locus_tag="ACGE36_001804"
     CDS             7423..8427
                     /locus_tag="ACGE36_001804"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8384186.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8472..10244)
                     /locus_tag="ACGE36_001805"
     CDS             complement(8472..10244)
                     /locus_tag="ACGE36_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384187.1"
                     /translation="MLSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFK
                     FDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQ
                     EESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSV
                     PQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGIL
                     FEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTP
                     AELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTA
                     LGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQL
                     RQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVT
                     NLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLY
                     RKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGT
                     LTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3293 a         1695 c         2079 g         3179 t
ORIGIN      
        1 aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac aaaactgtta
       61 gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat tagttacact
      121 ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa tccaaaattg
      181 aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact gctgttcctg
      241 atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa cgaattttag
      301 gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg gaataaatga
      361 acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat tgaaacaagc
      421 atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac caattgtcgt
      481 ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca aatctggcca
      541 tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga tctttcgggt
      601 ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc cttcggttcc
      661 tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat tttatcccag
      721 aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta ctactggaga
      781 accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat ggacgatgga
      841 agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt cttttgattt
      901 atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc cgttaaataa
      961 agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga agttgaatgt
     1021 gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat tcaatggaga
     1081 aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga aaaaaactgc
     1141 agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg gtccaacaga
     1201 agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata gttatgaccg
     1261 actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag aaggtaaacc
     1321 tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat caaaaggtta
     1381 cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact tagcaagtta
     1441 ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg aaggacgtat
     1501 ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg aacattatct
     1561 gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag gaacaaaagt
     1621 acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg attttcagtt
     1681 gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga ttcctcagaa
     1741 gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc gaaaaaaatt
     1801 aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc agagccgttt
     1861 tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt gatatgggaa
     1921 aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt cagctttgga
     1981 ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca gacgatactt
     2041 gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt ttttgccgcc
     2101 gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt tgaacatgga
     2161 aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc tgtacaaatg
     2221 atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta tatccagttt
     2281 ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag atttgaaaaa
     2341 gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa aggcgtccaa
     2401 aatatttttg tcggtttttt atataaatac attatcggtt attattttgg tcaagtactg
     2461 cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc attagtaggt
     2521 tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag cttatttgca
     2581 gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa acttttttta
     2641 tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt ttggttccgt
     2701 gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt taaaagcaag
     2761 atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat atggcatgga
     2821 ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg tctgacagat
     2881 gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa atttacgcat
     2941 ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat tttttcagga
     3001 tttttagata aacttttttt tagctaacga atagtgacat attaatttat ataacaaagg
     3061 agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca ggaacagatg
     3121 aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat tctttagcat
     3181 ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct gtatctgaat
     3241 tcgagcgaga ggattggagc acaccgaata aaatcattca acaagcaact gcattgaagg
     3301 gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag ctgttggctt
     3361 actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag aaacgattag
     3421 acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa aaaattatgc
     3481 attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca gaatcagtcc
     3541 atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat ttttgctggg
     3601 agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag gagatgctat
     3661 gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa atggtgtaaa
     3721 aactgattac tttaatacgt attattctga acttcaaaca tacgactggt tgttttcaat
     3781 gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt tctcaaaagt
     3841 aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga agctaccttt
     3901 acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag aggatgaagt
     3961 attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt caaaacgatt
     4021 accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg gagaacgaga
     4081 aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta tccgagccca
     4141 cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc cagaattttc
     4201 tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat tgttcatcat
     4261 cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa tgttacaagg
     4321 atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa tagctgattt
     4381 tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag gctggaaagg
     4441 ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata ttactgctag
     4501 taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa taccagataa
     4561 actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt tatttttgaa
     4621 tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata agcaatggta
     4681 taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga agaacagcta
     4741 cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt aagagaagta
     4801 ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt aggcagtgtg
     4861 attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca tatggacgaa
     4921 gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca aactcttgga
     4981 ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg tgaagggaaa
     5041 atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca agtaagaaat
     5101 aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca agaagaagcc
     5161 aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata caaacgaatg
     5221 aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac agctgtttct
     5281 ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt cgcaggaagc
     5341 gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct tgtaaatccc
     5401 gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat gacaccaaaa
     5461 gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc atctatgatc
     5521 ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga tattccattc
     5581 caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt gacaagagat
     5641 ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca tacatcagtg
     5701 attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt gaaacggctt
     5761 gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga atacctattt
     5821 ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc aatacttggc
     5881 ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac ggtttcagtc
     5941 cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat aatttacttt
     6001 tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg ggtggcagac
     6061 caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg atacttggca
     6121 tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt ctgcccatgt
     6181 ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat gcggtaccta
     6241 cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg atagggatga
     6301 tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt gctgcatgga
     6361 caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc tggtttttat
     6421 tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc tggtatttgc
     6481 taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg aatgtgtcag
     6541 ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc tattttactt
     6601 agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa atctttgagg
     6661 gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct tataaaaatc
     6721 aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc gatgcaggta
     6781 gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat gctgtgatcc
     6841 ttagccatta tcatcatgat catattgctg accttggtgt attacagtat tactggcaac
     6901 ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca aaagattcca
     6961 ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat tttgaagctg
     7021 aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat ccagtagtat
     7081 gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt acaggtgatt
     7141 caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt ctagcagata
     7201 cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa gaagctggag
     7261 aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct cagtttggtg
     7321 atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt gatatagaac
     7381 ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt tgtaccaaat
     7441 gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca agaaatgcct
     7501 ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat cggcagaaac
     7561 caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat tcatgttgtc
     7621 cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa ctttagtttt
     7681 atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca gccaatcatc
     7741 caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa tgatctagtc
     7801 attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg gatgtttgct
     7861 catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt aaaagtaaaa
     7921 aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac gaatatcaaa
     7981 ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca agaaatcttg
     8041 ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt gactgatgaa
     8101 gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga ttggaattac
     8161 gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg ttcgattgaa
     8221 gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg cgatttcttc
     8281 ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata tgataaagca
     8341 tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc gatagaacca
     8401 gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac atatatggat
     8461 tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt gatccgatca
     8521 atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac gtctttcatc
     8581 atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg actaaatagg
     8641 tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt
     8701 atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc tctaaaggaa
     8761 tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg aagttcttcg
     8821 ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt ctggtaacgc
     8881 gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc gaaatttttt
     8941 gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt atgcattaaa
     9001 tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac ttcttggata
     9061 tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt taagaaatct
     9121 acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta gctgataata
     9181 acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg atagatacct
     9241 aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat ccaaggtgta
     9301 caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag tgaagtgact
     9361 aaagtgctta taacactagc aatattagca atcggcatta attcaggaag ttctgcagga
     9421 gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt
     9481 ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat gaaagctgac
     9541 gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa aagaatacca
     9601 cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca acttaagaga
     9661 ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata gaaaaaggtc
     9721 cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga ttgtgaataa
     9781 ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag tccagcatag
     9841 ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt tgctggtaaa
     9901 tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt ccgttggtat
     9961 aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat tagtaaattt
    10021 gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc tttatccacg
    10081 gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc ttttgtatag
    10141 aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga aaaaataaaa
    10201 taaaagaaat aagcaattcc tgttaaaaat aacaatgata atataa
//
LOCUS       DAXWLH010000091        10218 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXWLH010000091 DAXWLH010000000
VERSION     DAXWLH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.092"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(11..1519)
                     /locus_tag="ACGE36_001806"
     CDS             complement(11..1519)
                     /locus_tag="ACGE36_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFF8384188.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1678..1869
                     /locus_tag="ACGE36_001807"
     CDS             1678..1869
                     /locus_tag="ACGE36_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384189.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2072..2989
                     /locus_tag="ACGE36_001808"
     CDS             2072..2989
                     /locus_tag="ACGE36_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFF8384190.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2982..4055
                     /locus_tag="ACGE36_001809"
     CDS             2982..4055
                     /locus_tag="ACGE36_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384191.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4203..4721
                     /locus_tag="ACGE36_001810"
     CDS             4203..4721
                     /locus_tag="ACGE36_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384192.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4702..5094
                     /locus_tag="ACGE36_001811"
     CDS             4702..5094
                     /locus_tag="ACGE36_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFF8384193.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGESATTGAKEIKLNDGQSVIFNLSKL"
     gene            5170..6105
                     /locus_tag="ACGE36_001812"
     CDS             5170..6105
                     /locus_tag="ACGE36_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8384194.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6238..7017
                     /locus_tag="ACGE36_001813"
     CDS             6238..7017
                     /locus_tag="ACGE36_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8384195.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7010..7327
                     /locus_tag="ACGE36_001814"
     CDS             7010..7327
                     /locus_tag="ACGE36_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8384196.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7571..9181
                     /locus_tag="ACGE36_001815"
     CDS             7571..9181
                     /locus_tag="ACGE36_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8384197.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9338..10186
                     /locus_tag="ACGE36_001816"
     CDS             9338..10186
                     /locus_tag="ACGE36_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8384198.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3210 a         1793 c         2066 g         3149 t
ORIGIN      
        1 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
       61 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
      121 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
      181 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
      241 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
      301 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
      361 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
      421 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
      481 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
      541 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
      601 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
      661 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
      721 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
      781 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
      841 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
      901 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
      961 agcaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
     1021 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
     1081 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
     1141 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
     1201 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
     1261 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
     1321 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
     1381 gtacacgttt cgcatgcttg tcgtcaacta acttcccacc aaattctaaa tataatttgt
     1441 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
     1501 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
     1561 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
     1621 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
     1681 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
     1741 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
     1801 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
     1861 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
     1921 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
     1981 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
     2041 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
     2101 tgagccagac gaactagagg agacgaacgc ggatcaggat acaggacaag aatcgtcttc
     2161 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
     2221 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
     2281 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
     2341 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
     2401 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
     2461 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
     2521 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
     2581 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
     2641 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
     2701 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
     2761 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
     2821 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
     2881 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
     2941 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
     3001 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
     3061 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
     3121 aaatacatga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
     3181 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
     3241 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
     3301 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
     3361 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
     3421 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
     3481 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
     3541 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
     3601 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
     3661 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
     3721 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
     3781 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
     3841 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
     3901 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
     3961 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
     4021 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
     4081 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
     4141 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
     4201 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
     4261 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
     4321 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
     4381 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
     4441 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
     4501 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
     4561 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
     4621 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
     4681 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
     4741 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
     4801 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
     4861 tcggaaaaag aagtggtctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
     4921 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
     4981 aaaaatcaac tctattggat gtttactgtg gacggggaaa gtgctactac tggtgccaaa
     5041 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
     5101 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
     5161 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
     5221 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
     5281 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
     5341 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
     5401 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
     5461 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
     5521 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
     5581 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
     5641 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
     5701 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
     5761 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
     5821 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
     5881 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
     5941 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
     6001 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata
     6061 tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga
     6121 attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc
     6181 gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg
     6241 aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata
     6301 agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct
     6361 ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca
     6421 tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa
     6481 ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg
     6541 tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag
     6601 ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg
     6661 actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat
     6721 gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg
     6781 aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg
     6841 gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga
     6901 tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa
     6961 gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact
     7021 atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc
     7081 gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt
     7141 accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct
     7201 ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa
     7261 aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat
     7321 gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat
     7381 ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa
     7441 aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt
     7501 gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga
     7561 gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct
     7621 tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa
     7681 tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt
     7741 cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac
     7801 aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt
     7861 tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat
     7921 cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt
     7981 gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg
     8041 gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg
     8101 tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac
     8161 agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag
     8221 aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa
     8281 ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt
     8341 cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg
     8401 tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa
     8461 taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat
     8521 attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt
     8581 ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc
     8641 actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt
     8701 gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa
     8761 tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca
     8821 agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt
     8881 cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt
     8941 agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt
     9001 atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc
     9061 aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt
     9121 cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata
     9181 ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt
     9241 cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag
     9301 gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga
     9361 tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat
     9421 ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata
     9481 ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga
     9541 agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca
     9601 tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag
     9661 cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt
     9721 agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat
     9781 cgatcgaatc cgagataata cctatgctta tctgattgta gatgatttga acaatttagt
     9841 acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc
     9901 gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa
     9961 agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt
    10021 caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt
    10081 gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac
    10141 acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa
    10201 aaaacatacc aaaaatat
//
LOCUS       DAXWLH010000092        10108 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXWLH010000092 DAXWLH010000000
VERSION     DAXWLH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.093"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(65..262)
                     /locus_tag="ACGE36_001817"
     CDS             complement(65..262)
                     /locus_tag="ACGE36_001817"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8384199.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(408..1286)
                     /locus_tag="ACGE36_001818"
     CDS             complement(408..1286)
                     /locus_tag="ACGE36_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8384200.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1325..2443)
                     /locus_tag="ACGE36_001819"
     CDS             complement(1325..2443)
                     /locus_tag="ACGE36_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFF8384201.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2440..4539)
                     /locus_tag="ACGE36_001820"
     CDS             complement(2440..4539)
                     /locus_tag="ACGE36_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFF8384202.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4590..5411)
                     /locus_tag="ACGE36_001821"
     CDS             complement(4590..5411)
                     /locus_tag="ACGE36_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8384203.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5408..6217)
                     /locus_tag="ACGE36_001822"
     CDS             complement(5408..6217)
                     /locus_tag="ACGE36_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8384204.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6238..6732)
                     /locus_tag="ACGE36_001823"
     CDS             complement(6238..6732)
                     /locus_tag="ACGE36_001823"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8384205.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6744..7244)
                     /locus_tag="ACGE36_001824"
     CDS             complement(6744..7244)
                     /locus_tag="ACGE36_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384206.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7309..10005)
                     /locus_tag="ACGE36_001825"
     CDS             complement(7309..10005)
                     /locus_tag="ACGE36_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8384207.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3072 a         2272 c         1659 g         3105 t
ORIGIN      
        1 ctttttataa tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa
       61 tctatcacca aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa
      121 tgaacataca ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat
      181 tactatttaa gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag
      241 aaaaaaggaa acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac
      301 ttagagcctt tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt
      361 actcggttaa atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta
      421 tttcttaccg cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat
      481 tcgggcgtaa taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca
      541 acaaatttat cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa
      601 ataattctat ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag
      661 gcttcaatct gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt
      721 ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt
      781 cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg
      841 aacccttcca cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg
      901 aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc
      961 gcattgacat ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca
     1021 aaattgaaat tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat
     1081 ttgcattgaa tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta
     1141 aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa
     1201 ctgactcttt ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct
     1261 ccagcttcaa ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac
     1321 ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt
     1381 ctgttgaata ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat
     1441 catcgttgcc gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt
     1501 gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg
     1561 cattcaattt tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat
     1621 accctaaaaa ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat
     1681 gaaaggccca cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact
     1741 cactatcccc atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc
     1801 aggtaagcgc aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact
     1861 tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc
     1921 tattttcttc caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat
     1981 ccttcggatg taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat
     2041 aggaattaaa gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct
     2101 ctccaaaccc attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc
     2161 catcccaata aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat
     2221 cacgaatcgt catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag
     2281 tttcttcacc agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc
     2341 ttggcaacat cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac
     2401 tctggatttt tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat
     2461 gtgaatcgtt tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg
     2521 cttttctgac aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc
     2581 ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa
     2641 tactcctttt ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc
     2701 cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc
     2761 taagcgatac ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc
     2821 taatcgggtt acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc
     2881 aggaaaagtg gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt
     2941 tggattgaaa gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc
     3001 taaaaattga taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa
     3061 attaattttt ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg
     3121 tttttgatag tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa
     3181 ttgctcacca aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca
     3241 ggcaatgttt ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc
     3301 aagtccaata caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc
     3361 aaaggcgtct gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc
     3421 taatatgtcc tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa
     3481 ttttgtatcc ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt
     3541 actcagagaa tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg
     3601 atcaaaccat agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga
     3661 tgcgtaatac atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct
     3721 gccgacgacc tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg
     3781 acgatagaga tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa
     3841 atagttcaat gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc
     3901 ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat
     3961 cgtgtcctta tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg
     4021 atacgaaggc acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta
     4081 aacctgttga acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac
     4141 aatgacctta aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc
     4201 gacttcaata cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt
     4261 taaggataac gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc
     4321 agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt
     4381 tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg
     4441 atagtagctg tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa
     4501 tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa
     4561 aactgcagcc tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt
     4621 cccaagacga ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg
     4681 aaacagctca atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct
     4741 tggacattca ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc
     4801 atactgatcg tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga
     4861 atttcaatta acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct
     4921 aaaacgccgc catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata
     4981 attggtgcca aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca
     5041 cgccaaactc cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt
     5101 ttgatcgcgt tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc
     5161 gatgccgcaa tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta
     5221 tgacgttcca gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc
     5281 atcatagaat aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata
     5341 gtcatcgagc gccaaaacat cttccgaacc aattttttct catctttaga aattccatca
     5401 gctaatttca ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat
     5461 cgctttgaac tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa
     5521 ttgcaataat cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa
     5581 caacggcccc aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca
     5641 tcgccatccc aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct
     5701 ctggaatatt atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg
     5761 gaataagagc ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc
     5821 ctttttgatc accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa
     5881 aagaaaaaat catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa
     5941 taatcgcatc cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca
     6001 aagaaggctc cgccgtacta gagattccga tattgacgac acccataaag atggcttcta
     6061 gtgacgcccc gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag
     6121 caacaacaat cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa
     6181 taaaaacggc caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta
     6241 aaaatcactc aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac
     6301 ggtatctgga taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat
     6361 cacttccttt gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc
     6421 cacgttcatt aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac
     6481 attagggact aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat
     6541 cgcgtcatca actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt
     6601 catcttttga atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt
     6661 tagactcttt gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac
     6721 tgctttaatc atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc
     6781 tcatcaagaa tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc
     6841 tcctttgtta attccttggt tgccaatact atttcgagca gaattggcag attaaagcca
     6901 gacaaaatag ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca
     6961 cttccgccaa ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca
     7021 aatacattca actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc
     7081 tcttgaacct tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg
     7141 tgagtcgcta ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata
     7201 taagcaattc ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa
     7261 aaaagtgtct aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat
     7321 atacggtcct aataattcat agagcagcga aagctcataa gcattgactt taaaattata
     7381 tttcatctcc attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg
     7441 tttcaaatca taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat
     7501 catcaatgcc gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata
     7561 gtaattttca tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag
     7621 aaagttcaaa cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa
     7681 gcggttaaat tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc
     7741 atcaccgtct aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc
     7801 cgaagtcgtg atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa
     7861 ttcacgtagt tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg
     7921 acgaaaaaca tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat
     7981 gttgctactt tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc
     8041 atcctccgca atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc
     8101 aatggaagtc gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg
     8161 tgtgtaaatt tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac
     8221 ttgccgtttc acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg
     8281 catatccaac gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct
     8341 gctagccgta ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac
     8401 ataatcagat aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag
     8461 cggtggtaaa tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc
     8521 taatgtacga tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct
     8581 gctaataatt tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga
     8641 tacccagacc tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc
     8701 cttatcaatg tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt
     8761 gaaattacct tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg
     8821 tttgattcga tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa
     8881 gctttctact tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt
     8941 taatggttct tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt
     9001 cagcttacca atattcccac taaagggatg agataacatt aaagacaagg cttctcttga
     9061 aacaataata tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc
     9121 taatcgttct cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg
     9181 ccgatcaaag gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg
     9241 aaccttagca gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat
     9301 aaatagaaag agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag
     9361 atagccgttg ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc
     9421 ccccttcttg tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc
     9481 acagtttaag atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc
     9541 aaaaattttt gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact
     9601 gagtccgtta ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact
     9661 tccgtcatga ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac
     9721 cgcatattca tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt
     9781 atcttccact aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt
     9841 cgaaattttc tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa
     9901 aactcgatct ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata
     9961 ataaactatc ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac
    10021 acaaatagtc gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt
    10081 tttatctaat atgattttta tgtttttt
//
LOCUS       DAXWLH010000093        10092 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXWLH010000093 DAXWLH010000000
VERSION     DAXWLH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.094"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(244..756)
                     /locus_tag="ACGE36_001826"
     CDS             complement(244..756)
                     /locus_tag="ACGE36_001826"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFF8384208.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(770..3067)
                     /gene="recJ"
                     /locus_tag="ACGE36_001827"
     CDS             complement(770..3067)
                     /gene="recJ"
                     /locus_tag="ACGE36_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFF8384209.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3209..3652)
                     /locus_tag="ACGE36_001828"
     CDS             complement(3209..3652)
                     /locus_tag="ACGE36_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFF8384210.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3653..4441)
                     /locus_tag="ACGE36_001829"
     CDS             complement(3653..4441)
                     /locus_tag="ACGE36_001829"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8384211.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4438..5379)
                     /gene="rnz"
                     /locus_tag="ACGE36_001830"
     CDS             complement(4438..5379)
                     /gene="rnz"
                     /locus_tag="ACGE36_001830"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFF8384212.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5556..6143)
                     /locus_tag="ACGE36_001831"
     CDS             complement(5556..6143)
                     /locus_tag="ACGE36_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFF8384213.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6364..7677)
                     /gene="obgE"
                     /locus_tag="ACGE36_001832"
     CDS             complement(6364..7677)
                     /gene="obgE"
                     /locus_tag="ACGE36_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFF8384214.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7974..8984
                     /gene="gap"
                     /locus_tag="ACGE36_001833"
     CDS             7974..8984
                     /gene="gap"
                     /locus_tag="ACGE36_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8384215.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9069..10004)
                     /locus_tag="ACGE36_001834"
     CDS             complement(9069..10004)
                     /locus_tag="ACGE36_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8384216.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2781 a         2298 c         1747 g         3266 t
ORIGIN      
        1 tacgggggtg tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc
       61 cagtttttcg gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct
      121 ttgcttgttt ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact
      181 tttgagggtc gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc
      241 tgcttaatat tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag
      301 atccatcaat tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag
      361 ttcgatcgtc gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg
      421 ttgacctggt ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc
      481 ataagtcact tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc
      541 gactcctaac tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac
      601 taccatatca attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata
      661 agcgtcacca tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata
      721 gtcgggaata ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta
      781 accatttctt tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat
      841 tgagccgtcg ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt
      901 tttgtaaaac accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa
      961 aaattaataa tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta
     1021 tatcaattcg ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc
     1081 ctaccccatt caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg
     1141 aataagaccg aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta
     1201 gagagtttgg ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa
     1261 tcgcagcttc ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat
     1321 aaaggctacc ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata
     1381 cttgcagacc acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc
     1441 attcattgat cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt
     1501 gagtaccaaa accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt
     1561 tcagatgctg ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata
     1621 aaaactgagg caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc
     1681 gctctactgt tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca
     1741 tcaagtcaat ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat
     1801 tttcacttgg catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat
     1861 ctcgcatctg gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt
     1921 tagctgatcc atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta
     1981 ttttcccagc aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga
     2041 tatgattctc cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt
     2101 ttctttcttc attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa
     2161 acgtcgcaag taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc
     2221 tgggagcaat cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt
     2281 cttcaaatag cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg
     2341 aaacaaggat tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta
     2401 ctaaatccaa aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta
     2461 ctccgactcc tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa
     2521 tcgcataagc gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta
     2581 caccagcttg ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga
     2641 tgatcaactg gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc
     2701 catgctcaaa tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt
     2761 ctttcattac cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt
     2821 tttctccact gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca
     2881 gatacggatc atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct
     2941 catggtaacc tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga
     3001 tcatttggag aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt
     3061 gtctcacttt ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca
     3121 gaaagaaaga ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt
     3181 ttgtacgacc atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg
     3241 attgagtttt ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta
     3301 actccgcctg ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt
     3361 cttcttttgc ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag
     3421 tcagctgttt gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa
     3481 tcagtgcacc aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag
     3541 tgaagccaaa acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca
     3601 aaactaaaac aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt
     3661 tgttaaaaat tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg
     3721 atgcaacttc taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga
     3781 cgatcatttt tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc
     3841 ttccgcttgg atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg
     3901 taacagctac gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg
     3961 caaatttcgt ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg
     4021 cgatattaat gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca
     4081 aaaccatcat tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg
     4141 taaggaagtc ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga
     4201 cttcttccat caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct
     4261 tgaaagcata agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa
     4321 atgccattct tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga
     4381 tttgtgcgcc cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca
     4441 tgcctcttcc tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt
     4501 gttttcaaac acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct
     4561 cgcactgata tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc
     4621 ttgatgagtc gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt
     4681 gctttcatgt accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct
     4741 cgtatcgcct aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc
     4801 attgatcgtc cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag
     4861 tggtccagct ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc
     4921 atgttcaacc acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac
     4981 agaaaattgc tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg
     5041 ataacttaaa cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt
     5101 tggcccgtaa atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc
     5161 gggaagtcca aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct
     5221 cggacgaata gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca
     5281 aacttcattg cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc
     5341 aggaacacct gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa
     5401 gttcctcttt aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt
     5461 caaattaaaa ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag
     5521 cagcgaaccc gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca
     5581 ttgtcgttgg aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca
     5641 tgattttcag cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga
     5701 gtaatttctc cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca
     5761 tcagcaatca agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac
     5821 acggtctggg catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca
     5881 aaccatccta ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat
     5941 ccacccgtag aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga
     6001 tgtgtcccat taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca
     6061 gcatgtaact ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt
     6121 aaaatgatta ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt
     6181 tacctttaat tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg
     6241 tgacaaaagt cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac
     6301 ttctgtcaca acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct
     6361 tgcctattcg acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc
     6421 accgcgtgcc cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat
     6481 cactgtttca tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga
     6541 taagatccac gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa
     6601 tccatagtgt acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac
     6661 ttctaacaaa tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga
     6721 aattggaaat actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc
     6781 tttgaatttc actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat
     6841 ttgcggacgc tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata
     6901 gtcttcataa ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg
     6961 tgttctttcg atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc
     7021 aatcaagcct ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc
     7081 tagattaggt acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat
     7141 aacggatagt aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa
     7201 tactttcaat tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc
     7261 cggtgcagga ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt
     7321 tgccacaact agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc
     7381 atctcggaca gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg
     7441 catcccttta ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa
     7501 atccatcaat gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc
     7561 gtctccgcca gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc
     7621 gtctccgccc ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat
     7681 taagtcctcc tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag
     7741 tcttacttat tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt
     7801 accttcttct ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata
     7861 attttttgac aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattgt
     7921 attaattgag gatgccgttt cacagcaccc ctaataaagg aggaactttt gatatgacag
     7981 taaaagtagg tattaacgga tttgggcgta ttggccgtct ggctttccgc cgcatcaaag
     8041 aagtctcaga tgatattgaa gtagtagcaa tcaacgactt aacaagtcca actatgttag
     8101 cacaattatt gcaatttgac tctactcatg gtacttatcc tggcacagtt actgcaacag
     8161 aagactcaat cgtagtggat ggcgaagcaa ctcgtgttta tgcagaaccc gacgcaagca
     8221 aaatcccttg ggcaaaagaa aacggtgtag atatcgtact agaatgtact ggtttttata
     8281 catctgaaga aaaagcaaaa gcgcatttag atgctggtgt caaacgagta gttatttctg
     8341 ccccagctgg tgctatgaaa accatcgtct ataacgtcaa tgatgataca ttagatgaaa
     8401 acgacaaaat catctcagca ggttcatgta caacaaactg tttagcacct atggcttatt
     8461 tcttaaataa tgaatttggg atcgaagttg gtacaatgac aacagttcat gcctacactt
     8521 ccacacaaat gctcttagat ggacctgtaa aaggcggtaa tttgcgtgct gctcgttcag
     8581 ctgctgacaa tactatccca cattctactg gtgctgcgaa agcaatcggc ttagttatcc
     8641 ctgagctaca aggaaaatta caaggacacg cacaacgtgt tccagtcgtt gatggttcat
     8701 taactgaatt ggtttcagta ttgaaaacaa aagtaactgc tgatcaagta aacgaagcaa
     8761 tcaaaaaaca cacagttgac aatccttcat tcgggtatga tgaccgtcaa atcgtttcta
     8821 gcgatgtcat tggaacaact caaggttcga tctttgaccc aacgcaaaca gaagtaacaa
     8881 ctgctggtga tttccaactt gttaaaactg ttgcatggta tgacaatgag tatggattta
     8941 cttgccaaat gattcgctta ctagaaaaat tcgctaactt gtaatttgat agatcaatcg
     9001 ataaaagaag cctttgactg gatccagtca aaggcttctt tcttttcatc ccctactttt
     9061 aaactcgttc aatgaacata ttggcttaaa aattgttgga ctacttgctg atatttttca
     9121 ggatctgtct tgtatgcttc cgcatgctcg gctcctttta caacatactt ttcttttggt
     9181 cccttcgttg cacgatagac cttatctaac atttcaaatg gtacaaatgt atcttcgtct
     9241 ccatgtataa agagcattgg gcgctggttt ttcttcaatt gttccactgc atttgcttca
     9301 ccgaaaaagt agccagcacg gacttttgtc actagactcg ttacttgcat aagcggaaat
     9361 gctggaagac taaacatatc gtctagctga taagcaagct catcatgaac ggaagaataa
     9421 ccgcaatctt caatgattgc tttcacatta tctggtagtt tttctccgct agtcatcata
     9481 actgttgctg ctcccatgct gacaccgtat agaacgattt cttgcgattt tccattgttt
     9541 tctagcactt tgtttatcca ctgtacatag tctttacgct ctggccatcc aaatccaatg
     9601 taatcccctt cgctttttcc atgtcctctt gcatcaggaa caaggacatt gtaacccata
     9661 tcatgaaaca tctttgcgta attggtcatc gtttcagcgt ttcccatata gccatgagcc
     9721 acaagagctg ttttttcact tttcgtttca gcaggaagat aaatcgctga taatttcaga
     9781 ccgtcttttg aagtgatggt ccaatcgctt cggttttctt ttgaactaaa ccatttatgt
     9841 gcatccgttt ctttggatgt ctgtgttgta ccaggtgtat cttcgtctaa aaagtctttc
     9901 tcagaaggca caacagcata attataaaaa taattccctg caaacactaa cgcaacaatc
     9961 agagctaaag cgacgcctaa aaggattttc atccaaagtt tcataaatca tccatctcat
    10021 ttctcttttt tcatataacc ttgaattagt ataatcaaag aatctctact atgcaatgtt
    10081 catttttata ac
//
LOCUS       DAXWLH010000094        10022 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXWLH010000094 DAXWLH010000000
VERSION     DAXWLH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.095"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..197
                     /locus_tag="ACGE36_001835"
     CDS             <1..197
                     /locus_tag="ACGE36_001835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384217.1"
                     /translation="KEVATRLLSQPLINGVLLPTLRSQVKIFIKHEKLFFEIAPCDSE
                     LNGRLTTSDINGVQSTDPRY"
     gene            complement(305..1288)
                     /locus_tag="ACGE36_001836"
     CDS             complement(305..1288)
                     /locus_tag="ACGE36_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFF8384218.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(1704..2474)
                     /locus_tag="ACGE36_001837"
     CDS             complement(1704..2474)
                     /locus_tag="ACGE36_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384219.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(2467..3402)
                     /locus_tag="ACGE36_001838"
     CDS             complement(2467..3402)
                     /locus_tag="ACGE36_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384220.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(3996..5645)
                     /locus_tag="ACGE36_001839"
     CDS             complement(3996..5645)
                     /locus_tag="ACGE36_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8384221.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(5840..6736)
                     /locus_tag="ACGE36_001840"
     CDS             complement(5840..6736)
                     /locus_tag="ACGE36_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8384222.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(6729..7409)
                     /locus_tag="ACGE36_001841"
     CDS             complement(6729..7409)
                     /locus_tag="ACGE36_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8384223.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(7410..8192)
                     /locus_tag="ACGE36_001842"
     CDS             complement(7410..8192)
                     /locus_tag="ACGE36_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8384224.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(8185..9102)
                     /locus_tag="ACGE36_001843"
     CDS             complement(8185..9102)
                     /locus_tag="ACGE36_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8384225.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(9105..9389)
                     /locus_tag="ACGE36_001844"
     CDS             complement(9105..9389)
                     /locus_tag="ACGE36_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384226.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         2977 a         2183 c         1748 g         3114 t
ORIGIN      
        1 aaaaagaggt tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa
       61 ctcttcggtc acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc
      121 cttgtgactc agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa
      181 ctgaccctcg gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg
      241 ttcttattac ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa
      301 gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc
      361 gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa
      421 atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt
      481 ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg
      541 tatgttccct cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca
      601 tgatgccatc aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat
      661 ctccattgat cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag
      721 gaatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag
      781 aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt
      841 ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa
      901 tcaattgtgc cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta
      961 cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag
     1021 caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct
     1081 gttcatcttc tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga
     1141 aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga
     1201 catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg
     1261 gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg
     1321 aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag
     1381 tgaacagctt aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc
     1441 acagtccatt ttccggaata aatggtgttc aaaagcttgc atttgcggta ataagtccaa
     1501 ccaaacgaaa aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac
     1561 agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc
     1621 aaaaaacaac aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa
     1681 acgctgattt cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac
     1741 caagtaacgg cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact
     1801 ggcacatcga tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga
     1861 ttgacttttg aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca
     1921 cctagataag ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac
     1981 tgagcaaaaa tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat
     2041 gtaatgatga ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc
     2101 gttacgagag agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata
     2161 atcacatgcg taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga
     2221 gagaagaagg acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa
     2281 ccaggaacga tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc
     2341 atttttccga aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg
     2401 acccatatac gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa
     2461 taaagattaa acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag
     2521 ttcttctaaa cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag
     2581 ctgatcgaag attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc
     2641 atcttcaaat gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc
     2701 aaagataaat gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat
     2761 cagttcaccc gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata
     2821 atgcgtcgtc aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat
     2881 ctcgcgtctc aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg
     2941 ttcatgcatc aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg
     3001 tgcacggaca tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt
     3061 catcgcttct ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac
     3121 cgtttcaaaa gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg
     3181 aactagatcc ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac
     3241 taaagaagta ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc
     3301 atagaaatct ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc
     3361 atagatcttt ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta
     3421 tagacatgat tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca
     3481 cacaaaaaca cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga
     3541 tagaataact atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact
     3601 actacgatga catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta
     3661 agctttcctc cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa
     3721 atggaaatag ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat
     3781 ggaacgtgcg tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac
     3841 agcgtggttt attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac
     3901 tcgttcccat tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct
     3961 ttttggaccc acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc
     4021 tgttcatcga acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc
     4081 aaaactttca gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa
     4141 tcaaaactga ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa
     4201 ttctttaaaa tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac
     4261 tccgcaagcc accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct
     4321 tttaaaatct catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa
     4381 cgtttgatcg atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg
     4441 attgccatgg aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt
     4501 tggatcgtct gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct
     4561 gatgcattgg aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct
     4621 tcttttaaca atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt
     4681 cggcaagtga tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct
     4741 cccagcaatt cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg
     4801 aaatgatagg agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct
     4861 gcttcactcc cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt
     4921 ttagtttgct gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg
     4981 gctgaaatcg ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc
     5041 ttcactagat aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt
     5101 acttgcccta aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat
     5161 aaacctacgc gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta
     5221 tgatcttttt ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt
     5281 tcctgataaa aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt
     5341 aaacgctgat acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat
     5401 acttcgaaca tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg
     5461 aattttttct ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga
     5521 taaggaattt ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg
     5581 tctaaaacaa ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg
     5641 cccatatccc tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg
     5701 aaaagggggt acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata
     5761 aaaacacctt ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact
     5821 tcaggtaaat tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc
     5881 cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa
     5941 gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga
     6001 agactttttc aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa
     6061 tgaatgaatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat
     6121 tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat
     6181 ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag
     6241 agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt
     6301 gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa
     6361 cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca
     6421 gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga
     6481 cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt
     6541 catccaccca tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga
     6601 ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc
     6661 tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca
     6721 acagccactt aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt
     6781 gcctttgcta gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca
     6841 aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga
     6901 tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca
     6961 ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc
     7021 gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac
     7081 agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa
     7141 actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt
     7201 cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc
     7261 aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga
     7321 agtttgtatc tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg
     7381 ataatcaaaa tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac
     7441 attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct
     7501 tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac
     7561 tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc
     7621 atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa
     7681 gccactggaa cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga
     7741 acataatttc catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa
     7801 gcaatcatgg ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg
     7861 agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc
     7921 gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga
     7981 atccatgcag ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc
     8041 gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa
     8101 aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact
     8161 tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa
     8221 ataatcttcc agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac
     8281 caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct
     8341 agattggaca aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt
     8401 cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa
     8461 ttctttgatc aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc
     8521 atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat
     8581 gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat
     8641 caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa
     8701 agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc
     8761 ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc
     8821 agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc
     8881 ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat
     8941 aatagttctc ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat
     9001 tccacgtttg atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt
     9061 tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta
     9121 ttgataagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag
     9181 tgtaaatttt ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt
     9241 gtctgccaat caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta
     9301 atcgttgtta tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc
     9361 cagaccatat aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa
     9421 tacatgccgt ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt
     9481 cacaatccct tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     9541 atatttatca aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc
     9601 acttctgtca caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt
     9661 cccgtttgtt tatagatcga atgatattat agaccaagaa tcctacctat taacatagct
     9721 aaaaacacaa aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg
     9781 taactgcgct cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat
     9841 aatcgcaatt aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt
     9901 ttctttatat cattgaatag ccttgaaaaa ccttgatata aaaaggtttt agtaaaaatg
     9961 attttatcaa atttgaataa ctttgaaata tttgtgttct aaaatgtgtt ctaatttaaa
    10021 gg
//
LOCUS       DAXWLH010000095         9918 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXWLH010000095 DAXWLH010000000
VERSION     DAXWLH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.096"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(11..1522)
                     /locus_tag="ACGE36_001845"
     CDS             complement(11..1522)
                     /locus_tag="ACGE36_001845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8384227.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1844..3196)
                     /gene="murF"
                     /locus_tag="ACGE36_001846"
     CDS             complement(1844..3196)
                     /gene="murF"
                     /locus_tag="ACGE36_001846"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFF8384228.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3349..4425)
                     /locus_tag="ACGE36_001847"
     CDS             complement(3349..4425)
                     /locus_tag="ACGE36_001847"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFF8384229.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4621..6366
                     /locus_tag="ACGE36_001848"
     CDS             4621..6366
                     /locus_tag="ACGE36_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFF8384230.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6646..7083)
                     /locus_tag="ACGE36_001849"
     CDS             complement(6646..7083)
                     /locus_tag="ACGE36_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFF8384231.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7067..8440)
                     /locus_tag="ACGE36_001850"
     CDS             complement(7067..8440)
                     /locus_tag="ACGE36_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384232.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8762..9250)
                     /locus_tag="ACGE36_001851"
     CDS             complement(8762..9250)
                     /locus_tag="ACGE36_001851"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8384233.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9272..9874)
                     /locus_tag="ACGE36_001852"
     CDS             complement(9272..9874)
                     /locus_tag="ACGE36_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8384234.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2894 a         2116 c         1796 g         3112 t
ORIGIN      
        1 cattttatgt ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta tcattgtgac
       61 gtttaccgcc atctttttgg ctgttattat ttttgctata accgcctttt gatttgtttc
      121 cacgataatt accgccgtca cgacgattac ggttgtttcc gccaccacgt ttgccatttt
      181 tattgtatcc ttttttctgc attggcaatg ggcgttctgg tgtgatcttc actggcactg
      241 catcagctgg atcttttgct gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat
      301 attcttctaa taatttatct gctgtttgca ggtatttatc tagaccattt tctgcaagtt
      361 ttgtttcgat ttgttcaact gcagcaccta attgaccttt gaatgcttct ttttcggttg
      421 gtggacgtaa agtcgtcatc cgttttttcg tcaagttttc aatcacatgc aagtagtcca
      481 tttcatttgg tgtaacgaaa gtcacagaca taccgccttt tccggcacgg cctgtacgtc
      541 cgatacggtg aacgtagctt tctggatctt gagggatatc atagttgtac acatgagtga
      601 cacctgaaat atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac
      661 cgctcttaaa tgaacgcaat acgctcattc gtttttgttg agacaaatcg ccatggattc
      721 cttctgcacg gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg
      781 tacgtccgaa gacgattgtt aattcaggtg tttgaacatc aaacaagcga gtcatgatat
      841 cgaatttttc gtattctttt gcgcggacat agtattgatc gatcaagtca gctgtcattt
      901 ctttcgcttt gatcttcaca tgatgtggat ttttcatgaa tttaacgcca atattcttga
      961 tagctggcgg catcgttgct gagaaaagta atgtttgtct ttgatcagga acttgtgaaa
     1021 tgattttttc gatatcttcc aagaagccca tattcaacat ttcatctgct tcatccaaaa
     1081 ctaatgtttg aacggttcca agtttcaatg tgtggcggtt aatgtggtca agcatacgtc
     1141 ctggtgtacc cacaacgata tgtggacgat ctttcaagcc acggatctga cggccaatat
     1201 ctgctccgcc gtaaacggct tgtacacgta cttttttgtc gcgccctaaa cgatataatt
     1261 cttcttgtgt ttggatagct aattcacgtg ttggtgcgat aactaaacct tgtaattcgt
     1321 gacgatctgg atcgattttt tctaacattg gtaatccaaa tgcagctgtt ttccctgtac
     1381 ctgtctgtgc ttgtccaata acgtcttttc cagctaatgc tagtggaatc gtttctgctt
     1441 ggataggcgt tgcttcttca aaaccagcgc gttcgactga tttcaacaat tctggtgata
     1501 attctagttc tttaaatttc aaatgaatcc tcctatatgg ttgttaactg aggatgtagc
     1561 ttttttgcta ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc
     1621 acgcacgttt ttatccattc ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt
     1681 ttaggcatta gaaaagccaa acgggtcgcg ttcagcttta tcattattat gacatctggc
     1741 ttcaacaagt tgtttctatg gcaaaaccag tccaatttct cttcatcttt cggcaatagg
     1801 ttattctaac atagtttttc gttttctgca agtttacaga tttttatttc atttgctgaa
     1861 gttgttctac cacttcaaat aagcccatgc tgttgctgcc ttttagcata atactatctg
     1921 ttgctgcaag attttgtttg atagcatcaa tcaatttatt cttttctttt tcaaaataga
     1981 acacaggtag ttgggggtat ttttggttca attcttcttt tagtgcatgc atttgttccc
     2041 cataaaggaa aatcatggcg aatttctcag gatcgatatg ttcactcatt tgcgcatgca
     2101 tatttaaaga ctctttccct aattccagca tatctgctaa cacagcgatt ctgcgtcctt
     2161 ttgttggcag atttttaaaa gtatctaata ccagtcccat ggctgttgga tttgcattgt
     2221 agacatcgct caaaatagct gcaccatttc ctgccgttag ccattcggtt ctgttttgag
     2281 tgatagaaac atgggcgagc ccttggcgta tctcaggtat ggacagtcca aagaagcgcc
     2341 cgatcgtata cgcaatcaaa gcattcgtta cattataact tccaggcagc gggatcgtaa
     2401 atgtttctcc ttctactaca aaatcggttt gtgcttttcc ttttgctgtc acttccgcat
     2461 gaacatcgcc atttccaata ccgaatgttg tgactgtttg tactaatgtt gccgttaaag
     2521 gtgtcagcaa cggttcatct gcgggcacaa cgagtaatcc gtctgataca agtccgtcag
     2581 tgatttccat tttggcttta gcgattcctt ctcgcgaccc aagattttct atgtgtgctt
     2641 caccgatcat tgtgattgct gcagcttttg gttgagccat aagtgaaagt tccgtgattt
     2701 cattggcgtg atccatgccc atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca
     2761 aaatcgtgta aggaagacca atattattgt tgtaatttcc ttgcgtctta tatgttttgt
     2821 atttttcaga caagacagac gcggttagat ctttcgtggt ggttttacca ttgcttcctg
     2881 taattgcaac gacttctgcg cctacttttt ccaagtaata ggttgccaat tgctgcatgg
     2941 ctttcaaagg atctgctaca ggaataactg gaaaattttt tggagcttgt tcaacaggca
     3001 agctccaaaa tgaagcagat gctccgtttg tgatcgcttg ctggataaat tcatgtccat
     3061 ctcgcgtacc agcaagagga acgaataaac ttccttcggt tatcttccgg ctgtcaaatt
     3121 cgacattggt aatctttgtc agtgcagagc attcgctgcc cacggcttgt gcaatttctt
     3181 gtattgttag attcattgag atctctccta aaaaaggttg tgaaaaaagc gtttggccta
     3241 aacggcgggt tcgcatgact ctcacttcca agtaaatttc tctattcgta tttagaagct
     3301 agaatgaacg cggttacgca atcactccag cctcttcttt atttctcttt attcattttt
     3361 ttcaaaaaaa gattgacgct gatggtatcg attcattcct aactggatca gttcttcaat
     3421 caaatcaccg tatttcaagc ccatattttc ccataagagt gggtacatac tgaactccgt
     3481 aaatcctggc atagagttta attcattcag gaataattca tttttatttg tcaaaaagaa
     3541 atcgcaccgg ctcaatccgc ttccacctaa catcgtgtaa gctaacttcg cgtactcttg
     3601 cgctttttga taaacttctt ctggcacttc ggctggaatc tgcatttcga ttttattatt
     3661 gatatatttt gcttcataat catagaatgc tacgtctttt acgacttcac caggcaaagt
     3721 cgtccgaacg tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt caattccttg
     3781 ttcaacgatt gctcgagaat catactgata ggctgttgct aaagcatttt gcagctcttc
     3841 tcggttttct gcttttgtaa tgccgacact agaacccata ttcgccggtt tgacaaacat
     3901 cggataaagc aaagaacctt cacattgatc aaatactttt ttaggatttt ctttccattg
     3961 attcttaagt actggtacat aaggaacttg cggcacacca gcagcttgta aaatatactt
     4021 ggtcatgatt ttatccatgg cacatgcact ggtcaatacg cctgcgccga cataaggcat
     4081 attcaatgtc tctaagaagc cttggatcgt tccatcttcc ccgtttggcc catgtaaaac
     4141 tggaaaaacg atggctcctt cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa
     4201 gccttcctct gtctgtccac ttgggtccca tgaaagatgc aagacatctt tgctggcagg
     4261 tttttctgtt agtaatggac ctttgaccca ttgtccttct tttgtaataa atacgagttg
     4321 aacttggtaa taattataat aaatggcatt taaaactgaa aatgcggaaa gaatggacac
     4381 ttcatgctct gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat tgtcctcctg
     4441 tattctaaat cgttcagtga tttactctca tattttagca caaaacaaaa ataaacgaat
     4501 aacttattta taaaaaaacg agccagcttc taaaagaacg agcgtcattt attttttgtc
     4561 agctagatta tttgtcccgt tttttataaa aagttatgat aaagaaaagg agggatgatg
     4621 atggcgcaaa caaatccacg cagccgcttg gcagaaatca ttcgagtgtt catcaattac
     4681 aatgtagtgc caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa
     4741 gaactaggcc ccaccttcat caagatagga cagatgcttt ctgttcgaga agatttgctt
     4801 tcttctgcct tcactcaaac attcaaaaca ttacaagata gtgtacctag cgacacattt
     4861 tcaacggtca aaaagacgat cgaaacagaa ctttctcttt cattatcaga tatattcgat
     4921 gatttttcaa aatcgccttt tgcctctgct tctatggggc aggctcatcg tgccaagctg
     4981 aagaacggtg acgcggtagt cgttaagatc caacacccta acatcgccga agagatccga
     5041 cttgatcttc aactatttga acgagcgatc cctttgatca aatacattcc tgaaactagc
     5101 gtagttgatc taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac
     5161 ttcttaaaag aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag
     5221 atccgcagcc ccaagattta tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa
     5281 atgtctggta aaaacttgaa tcatttaatg aacgccgaaa acaaaactga aacttttatc
     5341 actggaattc aaaacaagca gctgaaacaa gaagttgcaa agcttttagt tgagaacttt
     5401 atgaaacagg tgtttgatga tggttttttc catgctgatc ctcatccagg gaatctgcta
     5461 ttccatgtac taacaaaaga agaacagaca caagctagcc gaaaaactga aacagtccat
     5521 gaaaaagagt tcggttcctt tgcttttcga gcctccactt ctgcagaaga ccctgttgcg
     5581 ccatatacca tcaactacat cgattttggt atgatggggc atctttctgc aggacttagg
     5641 caaaaactca cacaagccgt tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa
     5701 gcggtccttc gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa
     5761 ctgacaagtt ttcttgaaca gtattatgat tctcctattg atgaaatcaa cttgcaagaa
     5821 gtattcacac atgtcgtgac gatttgtcat caaaataatc tgcagttcga tcgtgatatc
     5881 acgcttttgc tgaaagcatt tgggacatta gaaggggtga tccgcgtcct cgatcctgaa
     5941 gtttccctca tggaagtcgc ttcgcctttt gctcaacatt attttctcac tcatctggat
     6001 gtagaagata cattgaaaca aagcggatta gacttattgg aagggatgaa ggctgcgcct
     6061 aagatccctc aacagctcca ccatctatta gaaatgtgga cttcgggaca aggaaaagtc
     6121 aatttggaat tgaaaaagca agacaaatta ttgagcagga tcgaaagtat gatcaatcgg
     6181 cttgttttcg gtatgatttt agctgcattg atcgtagggt cctccctgct tgtccaagca
     6241 gcacctgtag aaaacgcaga ggtcgtttcc ttattaggga tatttacgta tgctattgcc
     6301 gcattcgtca ttattttttt ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa
     6361 aagtagaaaa aggttgtgac agaaagaatc agcttcaaga aataagaagg ggttttacat
     6421 aaattgtttc tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa
     6481 acaccgagaa taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa
     6541 agggaacatg gaacacattt atcctgtttc cctgttccct ttttgatgta ttcgagcata
     6601 aataaaatac cttcgttacc tactatcttc tgtacttggt aaaccttagt caattgcctt
     6661 ttttaacgct tgacgaaaag acatatgatc tttctccgta aagggttttg gaccctttgt
     6721 ccttatgcca gcttttctta actttccact atgataacgt tgttctagaa gcgtgccgat
     6781 attcgcttcg tcaaattctt ttccagagtg atggaacact cgcacaccct tcggcaatac
     6841 aagtgaagct aaaccataat cttgcgtgat caaaaagtca ttcttcttaa ccataccgac
     6901 gattttataa tccgcactat cagctccgcg gtctacgtag acgatttgga catgattagc
     6961 gtattctttg gtcgtataat ggtccacact ggtcacgata accacgtcaa gaccatagga
     7021 ttctcctaat tttatgacat cttcttttac tggagaacca tctccatcaa tgaatagcct
     7081 cattcttttt cgccttcttc cataaatgat aaacagttac taatccgctc agtaaaagac
     7141 caacattgat ccattttgcc aagataagca gttgtacact ttctatcggc actgattcat
     7201 ggttcagcgt gatcaataat agatggattg gctctcccat ttcttttgct aaaacaaaca
     7261 aaagcgtcat caataaaaac tggtggatca cactcccttt attttgagta gtatacttga
     7321 caacgatccg gcgatccatt ggaatagctg tatcagtttg ataatattcc ttatttagcc
     7381 agctgcctgt tactacgtgc cagaaactaa gcagaaagac acctatcgca aaaccatttg
     7441 gaatcaagag gaccaataaa gaagaactca gtccaagcaa ttgcatagcc aatggagacc
     7501 atgttaaacg tttcaaaatc catggtccga ctatttttgc tgcaatcaag cctgctgcat
     7561 aaggcaaata cataaaaatc gaaagttttt ctaatccgta cagtatgcca acataaaaac
     7621 tgctaaataa aaaaaggaaa ttccctagca ttccatttag aaaagtaaac agatttagcc
     7681 agcttggcaa cttccattcc ttcttctggt gtcctaaaac aaacacgaac aagatgaaca
     7741 gcagcaagaa accataaata gcaagatcaa aaagcctttc atcgaataac aatcgggcat
     7801 ttctcaaaag aaacaataag ccaaagaata tcaaaaataa aacaagctct cgcgtagcta
     7861 ccagatttct tttgatgtct ttgaaatcca gttcgtaacg cggataatga ctaaccgtat
     7921 ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg
     7981 caggcaactc aatcgcttga tacaggagaa atagccagat agctgcaaat aagtactttt
     8041 tcccatccat attgataaaa ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag
     8101 gtacaagcca tgctgcactc agccctagca aaacacttga aaaataaaat aaaggaaaat
     8161 aaaatacgcc taatattcca agcaatgaac cagcaccacc aatcagcaga gcgatcatca
     8221 gcaacgtgta actatctaga ccaaatcgta gactgttgac taaaaatagt ccagtcatcc
     8281 gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca attatgcgag tctgttagtc
     8341 ccaaaaataa aagataagga ataaaaataa ccgtgttact taagataaag ggcgttgttt
     8401 gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat gacaatggct ccttaaacag
     8461 tcttataact aatctgctct tcgattttag cattaaaggg gaaataaacc aattttacag
     8521 aatatattct tgtaaaaaaa aatacagaaa gaagcataag cttctttctg catttttacg
     8581 gtacttgatt actcaagcag acaagccctt ctccacaaaa gtcttgcccg acacccctgc
     8641 atccggcagg tgataaagat agaaatcaat tatcctgtgc ctccaaaatc aattaggagc
     8701 tctgtaaacc agagtccttc tcgacccgtc gcataaattt actttaacac agttcctatg
     8761 tttatgcgag agcttttttc attttataat cgaccatttg aaatccttga ccgatatctg
     8821 aaataagttc ttctgtaatc tcaaatcctt catgcttata aacctcgatt gcttgatgat
     8881 tcccctgatt cactcgcaaa aagatctctc tttcctttgt ttgcgcagca agttcttcat
     8941 accagcgaaa aactgaagaa gtcaatcctt gaccacgtac tttatttagc aaatataatt
     9001 tacttaaata aatttttcca tttatctctt cataagcagt gtaaccgaca gtcttttctt
     9061 cgctttttaa taaaaagtag gacacacctt ctgtcagttc tttttgaatc tgtttttttg
     9121 actgataagt tttcagcatg taatccactt gtttttgacc aatgatcggc acaaaagctt
     9181 ccggccatac ttcttgtatg actgccatca gctcatccag ttcattttct gttactttta
     9241 ttttttccat acacccacgt ccttttttac attataaatg accttgcgct tgatggaata
     9301 aaaagagcat gatcatgcta tacacaccca ccgaccatgg tttcaagaaa atcacaatct
     9361 gcataaattc cctaaaagat aatttcgtca aaccagctac tagacagatc aagtcgtctg
     9421 gcgcaaaggg aagcagcaag gtaacaacta gtactttttt aatatctttc gtttttcttg
     9481 tcagcaattc atcaaatttc tgatactttt ttggcgataa aataagctgg acaaaggcta
     9541 cgccatagta acggacaagc aaaaatccga ttgcttcccc aatcactagg ccaatacaac
     9601 tatataaaag cccaaatcca ttgccaaaca tcagcatgcc cgctactgag gagatccctc
     9661 ccggcaaaat aggaatgatc acttggatca cttgcaacag aacaaatatc atcactgcat
     9721 acttgccaaa ttgctggata aacgcttgca aagacgtgct cgatcgaaaa atcccatgat
     9781 gataagcata aagaatcaac acaaagaaaa aaaacagacc aataatgggc atgatctgaa
     9841 gacatttttg taaaactgat ccttttttag acatctatat tccccacatc ttttttcatt
     9901 tattaaaact gcctcctt
//
LOCUS       DAXWLH010000096         9896 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXWLH010000096 DAXWLH010000000
VERSION     DAXWLH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.097"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(359..583)
                     /locus_tag="ACGE36_001853"
     CDS             complement(359..583)
                     /locus_tag="ACGE36_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384235.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            774..3551
                     /locus_tag="ACGE36_001854"
     CDS             774..3551
                     /locus_tag="ACGE36_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8384236.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3632..4051
                     /locus_tag="ACGE36_001855"
     CDS             3632..4051
                     /locus_tag="ACGE36_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384237.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4063..4533
                     /locus_tag="ACGE36_001856"
     CDS             4063..4533
                     /locus_tag="ACGE36_001856"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8384238.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4550..5329
                     /locus_tag="ACGE36_001857"
     CDS             4550..5329
                     /locus_tag="ACGE36_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8384239.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5316..6137
                     /locus_tag="ACGE36_001858"
     CDS             5316..6137
                     /locus_tag="ACGE36_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8384240.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6154..7203
                     /locus_tag="ACGE36_001859"
     CDS             6154..7203
                     /locus_tag="ACGE36_001859"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8384241.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7216..8220
                     /locus_tag="ACGE36_001860"
     CDS             7216..8220
                     /locus_tag="ACGE36_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8384242.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8853..9872
                     /locus_tag="ACGE36_001861"
     CDS             8853..9872
                     /locus_tag="ACGE36_001861"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8384243.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3320 a         1644 c         2069 g         2863 t
ORIGIN      
        1 atcttagagg aataaataat agtggagaaa actatggttt ggcgcagatt ctatcaggtt
       61 tgatggaacg aagacaacct cccgacatca agtcaaaagc tacttgagat tttgatctga
      121 aaataaaaaa agaaaacaag gtaggaacat caatgtttct accttgtttt cgtaatcaat
      181 gggaaaaaac taagagtggt gtacgtaatc aataaaaagg atggtgacaa cagtacctct
      241 ttactctctg aaagcatcat aactcgtttt tcctcattat gccaataaat tgttgttata
      301 ccacatataa ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt
      361 attttgtatg gaaggattta tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg
      421 tttcggctat atattgcgta tctacgccaa taaaacgagt ttgttctgtc atagccatat
      481 aaagcaaagg caaaaataca actacagcga gataagatcc gatgatttct cgaaaagttt
      541 tccaggaagt atgtttcctg tttttatatt tatccgattt cattgaatag tcatagcctc
      601 cttattcttt catatgttga aattccagta taaaaactgg aaggaactgt acgtttttta
      661 gaataaaaat tataagggat aagtaggctg ctgtctataa taagatatat ttttcaattc
      721 tttattaaag attttattga caaaatgaat ccgttttcta tgatggagga ggggtgaata
      781 agttgttgaa gaaggaaatt ttatgttact tagagaacca gacagccttt ttcgatttag
      841 aacatatgaa cgaggtcttt acagcacaaa ggttagctga tcattttggg gtaaaaagaa
      901 acacagtcag tcattatttg aaccagttaa atgatgagca actactagtc aaaatcaatt
      961 ctcgaccagt gatttatttc cacaaagaag cttttgaaaa acaatttttt aagttacgca
     1021 cgaattttta tttttctatc gatgatctca aagaggaaca gccttttttc gcgcaaccaa
     1081 aagacttatt ctcttttatg atcggatacg acgccagttt gaaagaaagt atcgaacaaa
     1141 tcaaaacagc tctttactat ccagatggcg gattaccact tttgattaca ggcgaaagtg
     1201 gcacaggtaa aagttattta gttaatttgg tctatcagta ttgtctgctt catgatctgc
     1261 tagaagattc tgcaccattc atcactttta attgtgcaca atatgcagat aatcctgaac
     1321 ttttgactag caatttgttt ggacatgtta agggagcgta cactggtgca gaagaaaata
     1381 aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt tttagacgag gtacatcgct
     1441 taacaccaga aggtcaggaa aaactgttca cctatcttga tcaaggtgtg atttatcgta
     1501 tgggagaacc taatttgtca cgccggatca aaacacgtct cttttttgct acgactgaag
     1561 agattactag tcatttctta acaactttca tccgaagaat acctatccga atcgaactac
     1621 ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact gatttactct ttttttctcg
     1681 aagaacaaag aaaaatgggg agccctttgt cagtcagtgg accagctttg tcacttttaa
     1741 caagtcagaa attatccgga aacataggag agctgaaaaa tatcgtcaaa gtttcagcag
     1801 caaaagcata tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc
     1861 aaaaagaact acttgcgcag cctattacta aaaatacagc acagcaagga atctatatta
     1921 cgcaagacca gaccttagag caattaatgg agaagcaaca gcccgaacag cagcgaatcg
     1981 ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg ctatattctt
     2041 gcgaaggaaa attaaagcaa gaagtgattc aattatttga ttttttgctg tttgagacca
     2101 gtagacaaga aaaacacgag ctgctggttt attggacaca atctatccga gagacctttc
     2161 gacaaatgga aactgcctat caaatcaaat tcgatggaaa cagtgtctat gccttgagtt
     2221 actatctcta tcagcgaagc actatcaaat ggttaccgga agaacatgat attatccaac
     2281 taatcaaaga attagaacaa caagtttctc agtcttatcc atctagttat cactatgtcc
     2341 aacgaattct ggaattaagt aagatcaaat tagatattga agacacaagt atggatcgga
     2401 ttttactgac gatctatcta aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag
     2461 cgatgattgc tgctcacggt tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt
     2521 tattacaaga ggatctattt gaatcatttg atatgccact agatgtcaca ccacagcaga
     2581 ttgcttcaga aatccttgat tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat
     2641 tagtagacat gggatctctg aaagaaatcc atcagttttt taaaaaacag ttatccgtac
     2701 cgcttttgat tttgaataat gtgacgacac ctttagcaat cacagtaggg gaatgcctgc
     2761 aaaaaaatgt atcattagaa gagatagcag aagagactgt tcgacagatc cagccagaat
     2821 ggcgactgct ctatccagag gaaaacaaac ccaaagcact cattactact tgcttcactg
     2881 gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa aagcttacct acggcttgtc
     2941 agttgaaaat catcccatat gaatatcagc aattaaaaga taagaaaaac agtgacccat
     3001 tattttccat atacgaagtc gtaggaatga ttggcaccac agatcctgat atcaccaata
     3061 tcgactatct ttcattagaa gacctcatct cgggggaaaa gatgtctgta ctagctgaat
     3121 ggttggacac tactatgaat gcttcagaga aggaaatatt caatcagcgc atcattcgta
     3181 atttttcatt ggaaaaagta ctggattccg tgactattct agatacagag aaggtgatgg
     3241 gagaaatcga tcattttatg agagaattag aggtgcagtt gaatcgaagc atcagcaatc
     3301 ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat tgaacgattg atccgacaaa
     3361 tgccaatcga aacctatcaa ggattagaga aatggaagca gtgtcaaaaa gaagggttat
     3421 ctgctattaa atctgcgttt agtgtcattg aagagcatta tagtgtcatg atccccaatt
     3481 ctgagattgc ctatatctat gatattttga gtgaagatac agagtttctc tcgatagaag
     3541 aagaattttg aacataagca agttgttgcc actaattttg gcatgcaact tgctttttta
     3601 gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga gaaattatct tagcttccca
     3661 tggaaaattt gcttcaggga tattagattc attagagctg atctatggga aaaatcattc
     3721 tattactgta ttcgattgct atactgatga gaattttgat ttagcggaaa cagtaaggca
     3781 gctttttatg aggtacaaag ataaagaaat cattgttctg acggatcttt ttggcggaag
     3841 tgtcaacaac gaattcttac aatatatcaa tcgggatcat gtctatctcg ttgccggatt
     3901 gaatttgcca ctattgattg agctagtatc tcggttggac gctgaagtag agacaactgt
     3961 tttgatccag caggtgctaa ctgattcaaa aaaaatgatc cagttttgta acgagcggct
     4021 caatcagaaa atagaagaag acgaatttta ggaggaagaa aaatgattgt attgacaaga
     4081 gtcgaccatc ggttattaca cggacaagta gctttttcat ggacacaaac aataggcgca
     4141 gactgtattt tgattgctaa tgatgatgta cctacgaatg agatacgaaa gacaacgatc
     4201 aaattagcca agccacaagg agtcaaacta gtgatcaaat caattgatga ctcgattgcc
     4261 gcgttgaaaa gcggggtgac agataaatat aagttattca tcgtagtgga atcgatcgag
     4321 gatgcatata agctggcaga agcgtatcca gaaatcaaaa ccatcaactt aggagggatc
     4381 aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa atattttacc agaagaggag
     4441 gaacgcttga aacgattaat tgacaaacaa atagaagtag aaatccgtca agtaccagca
     4501 gataaaaaag tggatgtaag agaagttttg taagaaagga agaacctcaa tgattgtaca
     4561 agcagtctta ttaggaattg ttgcatttgt cgcacaatca gaatatgcac tagggacatc
     4621 gttgctttct cgtccgattg tgacggggct gtttacagga atcgtgttag gtgatgtaaa
     4681 aactgggatc attatgggcg caactttaga gttagcattt atcggatcgt tctcagtagg
     4741 tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta gggacggctt ttgctattac
     4801 agctggagcc ggtacagaaa ccgcgctttt actaggtttg ccgattgcta cgttgacatt
     4861 gatattgaaa aatatttact tagggctttt gattccaatt atgaaccata aagcagacaa
     4921 ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg cacttgcttg ccggtatcgg
     4981 tctttccttg atgcttggtg ttgtggtaat ggtttcttat atggtgggaa gcaatacgat
     5041 cggaaatcta ttaaatatga ttcctgactt cgttcaaaag gggttgtcag tagctacagg
     5101 gttgattcca gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc
     5161 accatatttt ttcttaggtt ttgctatagc ttcttactta gagattcctg tgaccggagt
     5221 agctattttt ggtgcgattt tagcagttgt ggtagtcaat attatgaatg tccgtcaact
     5281 accacttcaa acaactgcag gggaggtaga agaagatgaa gaattctgag atcaaaaaca
     5341 gcaaaataac gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa atggagtttt
     5401 cttggaatta tgaacgtcag atgaatatgg cttatgttta tgcaatgatt ccgattttga
     5461 aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat ctagagtttt
     5521 ttaacacgac cccacatatc gttacactga ttttaggaat cacggctgca atggaggaag
     5581 aaaataagga tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa acatctttga
     5641 tgggacctct tgcagggtta ggagattctt tcttttgggg aactttgcga ttgattgcca
     5701 caggtgtggg tacctcttta gccatgcaag gaaatatttt aggccctatc ttgttcattt
     5761 tgattttcaa tattccgcac attctgtttc gctatctagc aacaggttgg ggatatcgct
     5821 taggaacggg ttttttgaaa aagatccagg caaacggtat gatggaaagt ttgacattag
     5881 gcgcgtctat tattggactg atggtcgttg gagcaatgac tgccacgatg atcaccatca
     5941 atattccttt aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt atttttgatg
     6001 atatcgttcc aaatatctta tgtcttggag catttggcat agtgttttat ctattgaaga
     6061 aagaagtgaa accattaacg atcttgatcg gactagcttt ctttggaatt tttggttcgt
     6121 ggatagggat tttttaaaaa aggagcagag aaaatgttta cgatgcaaga ttatatctat
     6181 gaagaaaagg aagtactaag tacgatcttg aaaaagaatg atttttcgac aagagaacat
     6241 atgaaaaaaa cagtgaattt actgatctta gcaacgggtt catcttataa cgcttgttta
     6301 gcagcgaagc cagcattgga atcttatggt gatcttactg tcgatattca agaacctttt
     6361 tattttttca attatggaaa actttctcct tcaatcgata ctgtgattgc tgtgtcacaa
     6421 agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca agggttacct
     6481 gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc acaaattatt
     6541 gatttaggtg ccggggtaga atcagtaggc ttcgttacaa aaggctattc tgcgactgtt
     6601 ttacagttac tactcttagg gataggaatt ggaataagta aaaataaaat cagcaaaaaa
     6661 atagaacaag actacatgca acagttgcgc aaaataatca accatttacc agcgattatt
     6721 caaaaaacgg aagaattttt tgatggatat caaagtttat ttcgtttagc acagcgtttc
     6781 gtcaccattg gatatggtcc aaactgggga acagctaaag aagcagagac gaaattgact
     6841 gaaacaatcc gcgttccgtc acaaggattt gagttagaag cttatatgca cggcccatac
     6901 ctggaggcag atgcttcgca tttgctattc tttattgaag gggaaagtgt aaataaggaa
     6961 cgttcgcaaa agttgcaaag atatatgagt cggtacgttg gagagactct caccattacc
     7021 acaaaaaaag caagaaacga gaaaacactt ggattagcaa tagaatgtga tgaatatctg
     7081 tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa
     7141 ggtattgact tgaacaaaaa gatttttgaa gactttgata cagtcttgaa aagtaaatta
     7201 taagaggtgg aaatcatgct gaaattcaat gaagaagaac aaatcaaagc aataaaaggc
     7261 gcactagctt tacgtccgca agtagaagaa atcatcgata aactttatgc ggaaaaattt
     7321 gacgctgtct attatctagg aatcggtgga acgtatgctt cctcgatgca agctgtcact
     7381 tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga atattacact
     7441 actggaaaca aaagattaac aaaagattct atcgttattt tgtcctctgt taccggtaca
     7501 actcaagaag tagtgaaagc agtagaacaa atcaagaaag taggagcgac actgattgga
     7561 tttatcgata aagcgaacag taagttgtct cagctatgcg attttgtggt cacttatcca
     7621 gctcctggaa cagaacaaat caaatttttt atggcagcag atcgattgat gtatttgaat
     7681 ggagaattcg aagcttattc agaatactat gaacagctcg aacgttattt accaacaggg
     7741 ttagtggaag cagagaaaaa agcagatgca tttggcttat catttgcaga aaaacatcga
     7801 cgtgatagta tgcattattt tattggagca gggaatcaat ggggagctgt ttattcatat
     7861 gccatgtgtt actgggaaga acaaagctgg cttccatcga aatctatcca tgctgccgag
     7921 tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt atttttaggc
     7981 gaagatgaac agagagtgtt atctgaacgt gtggctaaac ttttgccaaa aatttgcagc
     8041 aattatacat tgatagacac aaaagactat ccagtagaag gtatcagtga aaaatatcgc
     8101 ggacgtgttt tgtctttctt attgatgcat gtagtgacac aacgcatcga tgctcatgtg
     8161 gagcagttga actgccatcc attagagata cgccgctact atcgtcagtt tgattattag
     8221 gatagactgg ataaaaagaa tataaaaaat aaagagagca gcgataactg ctccctttat
     8281 ttttttaaaa tattggcatc aattacgtta taaaacacat tttttgcatt atctacagtt
     8341 caacttataa tctgtttaat gggacatgtg ggtgccaacc attttttgtt atgcaatagt
     8401 actatttagt tgttgcatgg atccgtagta aaagtccaag tacaacgagt tgcgccagtt
     8461 tacatagcgt tttgtatcca gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa
     8521 cagagacaat ctgtccgttg caggacctga cagatttaga aatgttcaat ctgtctctta
     8581 tgtattttaa atcaatactc actaggagaa gtttcctaac ctcctaatag taatgctggt
     8641 agtaacattg tcaaaactgc ttcctcttag gaacaagcaa agcataacga gtgtaaaaat
     8701 aaaatgtaga ctattgtaac atatcattga ttatttcaag taaaaacgat aaaagaagta
     8761 aacaaacact taaaaacaat ggaaattagt cccaacttta agaaatgtat actataatgg
     8821 aaattatcaa ttatttattt ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc
     8881 ctggatcttg gggcactgct ttagcacaaa cattggcaga aaacgggcat gatgtccgta
     8941 tttggggaaa tgtacctgaa cagattgatg aaatcaataa gtatcataca aatcagcatt
     9001 ttttacctga tttgacgatc ccagaatcga ttatcggata taaagaattg gcagaagcag
     9061 tggataacgc agatgctatt ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac
     9121 aggaacttat tggtaaaatg aatactaagc caatcattat acatgcaagt aaaggactag
     9181 aacaagatac gcataaacga atctcagaag ttttggctga agaaattcca gaagaaaaaa
     9241 gacaggcaat tgttgtatta tctggtccta gccatgctga ggaagtggca gtacatgata
     9301 tcacaacaat tacagctgca agtattgatc aaaaagcagc agcctatgta caaaaactgt
     9361 tcatgaatga ttatttcagg atttatacaa ataatgatgt gattggcgta gaaactggtg
     9421 cagcactgaa aaatatcatt gcaattggtg ccggcgctat ccatggatta ggattcggag
     9481 atgatgcaaa agcagcaatc atgactcgag gattggcaga aatcagccgt cttggtgtcg
     9541 cgatgggtgc caatcctctg acatttatcg gtttgagtgg tgtgggggac ttgatcgtta
     9601 cttgtacaag tgttcattct cgcaattggc gtgccggtaa cttgttagga cagggacata
     9661 agctggaaga agttctagaa aacatgggaa tggtcgttga gggtgtggca acgacaaaag
     9721 cagcagtaga gctagctcaa cagctaggtg ttgagatgcc aattactcaa acaatctata
     9781 atgttttgta taatggagaa gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg
     9841 gaaaaatgga gaatgaattt tctcttcgtt aaatagatga tcaatagtta atcgtc
//
LOCUS       DAXWLH010000097         9862 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXWLH010000097 DAXWLH010000000
VERSION     DAXWLH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.098"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..523
                     /locus_tag="ACGE36_001862"
     CDS             <1..523
                     /locus_tag="ACGE36_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFF8384244.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2044
                     /locus_tag="ACGE36_001863"
     CDS             584..2044
                     /locus_tag="ACGE36_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8384245.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2055..4259
                     /gene="topB"
                     /locus_tag="ACGE36_001864"
     CDS             2055..4259
                     /gene="topB"
                     /locus_tag="ACGE36_001864"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8384246.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            4275..4424
                     /locus_tag="ACGE36_001865"
     CDS             4275..4424
                     /locus_tag="ACGE36_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384247.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4498..5091
                     /locus_tag="ACGE36_001866"
     CDS             4498..5091
                     /locus_tag="ACGE36_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8384248.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            5107..6006
                     /locus_tag="ACGE36_001867"
     CDS             5107..6006
                     /locus_tag="ACGE36_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8384249.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            6009..6494
                     /gene="ssb"
                     /locus_tag="ACGE36_001868"
     CDS             6009..6494
                     /gene="ssb"
                     /locus_tag="ACGE36_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8384250.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            6860..7120
                     /locus_tag="ACGE36_001869"
     CDS             6860..7120
                     /locus_tag="ACGE36_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384251.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            7561..7767
                     /locus_tag="ACGE36_001870"
     CDS             7561..7767
                     /locus_tag="ACGE36_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8384252.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7767..8018
                     /locus_tag="ACGE36_001871"
     CDS             7767..8018
                     /locus_tag="ACGE36_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384253.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            8033..8470
                     /locus_tag="ACGE36_001872"
     CDS             8033..8470
                     /locus_tag="ACGE36_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384254.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            8463..9170
                     /locus_tag="ACGE36_001873"
     CDS             8463..9170
                     /locus_tag="ACGE36_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384255.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            9335..9535
                     /locus_tag="ACGE36_001874"
     CDS             9335..9535
                     /locus_tag="ACGE36_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384256.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            9551..9730
                     /locus_tag="ACGE36_001875"
     CDS             9551..9730
                     /locus_tag="ACGE36_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384257.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         3617 a         1674 c         2044 g         2527 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc
      241 cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt
      361 aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 aacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgcc gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg
     1081 tttagatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc
     1141 gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca catataagca
     1201 tatagataaa aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca
     1261 gttaaaattt tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca
     1321 gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta
     1381 ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat
     1441 ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc
     1501 agtcgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca
     1561 agtgccaaaa gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc
     1621 agaagctatt atggccatac acaagacaga aaacaatctg caattaacga accaactggt
     1681 ttccgctcac aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga
     1741 agccattaat ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg
     1801 cgtggatggt acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca
     1861 gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga
     1921 atttatacct gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca
     1981 agaaatcaga aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga
     2041 gtgaaatcgt catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt
     2101 atgccgatag cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat
     2161 tgtttcctgg tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc
     2221 ccgatatgta cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta
     2281 cacattatca ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc
     2341 aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga
     2401 tcgcttggac gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt
     2461 ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa
     2521 aagagaccta tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg
     2581 ggatgaacgg tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct
     2641 tttccttagg cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga
     2701 ttagaaattt caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg
     2761 gcgcatttga tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag
     2821 attatttaaa agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg
     2881 aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta
     2941 aaatgaatca gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt
     3001 atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattacg gaaggggagt
     3061 atgcgtattt gttggatcat ttagatgaat acaagcattt tttaaaggcc gaatcaatcc
     3121 caacccctat tcatacgccg aatagtcggt atgtgaataa caagaaagtc caggaacatt
     3181 acgcgatcat tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac
     3241 tgcagcaggc catttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaaat
     3301 atacctatga agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg
     3361 gaaaagtacc gatagatcta ggttggaaga agttatttgg caaagagaac gacggaaaag
     3421 agaaggaaga agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc
     3481 tacaagtcct tgaaaaagaa accaagccac cacagcctta taccgaaggt acgttgatca
     3541 cggcgatgaa aacagctggg aaaaccgttg atagtgaaga agcgcaatcc atattgaaag
     3601 aagtggaagg aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa
     3661 aggaatatat caaggtagag aaaaataagc tcgtggtgac gaataaaggg attttgcttt
     3721 gccaagccgt ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa
     3781 gctacttgtt aaagatcggt gaacgaaaag gaacacaaac cacttttcta gccaatatcc
     3841 aaaaatttgt ctctcactta ttggaagtcg taccagggca aatccaatcc actgattttg
     3901 gatcgacgtt acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag
     3961 gaatttgtcc aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcatgta
     4021 cgtctgaagc ctgtgatttc aaactatgga caacgattgc caaaaagaaa cttacagcga
     4081 cacagctgaa agagatcatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag
     4141 gtcagaaggg ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg
     4201 aattttctga aaagaaaaaa acgaacttca agaaaagaac acgacgcact acaaaataat
     4261 ggagggttaa tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag
     4321 ccaaaattcg ttcagaacag gaaaaaatgc gtgccagcaa acgaagactg cagcaattga
     4381 agaaccagca gcggattcaa aaagaaatcg aattagatcg ataaattcct ttcttgttaa
     4441 tataaaacta acatgttata gttgtgttag ttttatatta actttagggg aagagtaatg
     4501 aaagacaaaa tccaatgtat ttttgataaa cataatggca acctatcctt gaaggatgct
     4561 agaagtgaag gattatcgcc tatgacgtta gatcgattag ccgaaaagga taaaatagaa
     4621 aaggtagcac caggtttcta taccttaaag gatgcgttcg tggatgaatt gtatctcgct
     4681 cagagtatct attccagagg gattttttct catgagactg cgctcgatct ttttcaagta
     4741 ggaacatata tccctaaaaa gatcaatatg atgtttccta aaggatacaa tgtatcaaag
     4801 gagaaattag atcagtatgc cattcaaccg cattacacta ataaagaaaa ttttgacctg
     4861 ggagtcacag aaacagaaag cttttacggc aataaaattt tagtctatga tttagagcga
     4921 acgttatgtg acatgtggaa tccaagatac aaagcaagcg tagaggtaaa acaagaagca
     4981 ttaaaagaat atatgacaag taccagtaga aatacaaata agctaagaag atacatggat
     5041 attttaaaat caccaaaaga aatgactttg tatatgttgc cactttattg agaggagtaa
     5101 agatgaatga caccgacaca atttaaagag aaaacaagaa atttcgcagc agagaaacat
     5161 ttaaacccac aaattgtcca aagatattat atgatggaaa aattattaga atgtattgtt
     5221 gaatcgcctt atcatgatga ttttgtatta aaagggggat ttctgattgg atcaaaatac
     5281 gggttagaaa accgttcaac cgttgatatt gatacgactt atcgaaattc gaaactaaca
     5341 aaagaaaagt tagaaactgt tttttctgaa ttaacagcta atcctacaaa agatggaatt
     5401 cgttttgaat taaagggatt aacagagacg agggaagccg attattatcc aggttttcaa
     5461 gcaaagttta ttgcttatct tgaaaatgca cgtgtacctt ttaaattaga tattacttca
     5521 ggtgattcta ttattccagc agcattgatg tatgaacaca aattaatgtt tgaagataaa
     5581 acgattcata ttccagctta tcctacggaa caaattctag ctgaaaaatt acatgccacg
     5641 tttagttttg gaaaagataa ttcgcgcatg aaagattttt atgacctgta tatgattccg
     5701 aaattagaac gtatcgatga gaaaaatttg tataagagtg ttcaaggaac attcaaggaa
     5761 agaaataatc aatcggtgtt taagcatttg tatgatcagg aaatgcctca attggtgaaa
     5821 gatgagggct tgagagaaaa gtggaaaaga taccaacaag aaaactattt tgccaaagac
     5881 atttctttcg aatctacact aacttccgtt gattccctga tgcaatcaat tgtagagcaa
     5941 gagaaggcag aacgaaaaca aaattttaag agatcacaac aaatgaatca agaaatggag
     6001 agataaagat gatcaataat gtaactttag tagggcgatt aactaaagat ccagatttac
     6061 gttataccgc aagtggcaca gcagtggcga ctttttccct agccgttaat cgtaatttca
     6121 cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag
     6181 cagaaacgat ggcgacatta gctaaaaaag gcagcttgat cggcgttgtc ggacgaattc
     6241 aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtggccg
     6301 ataacttcca actattagaa agtaaagcag cgacagaaag ccgtatgcac gctgatcaat
     6361 cgtccagcag tccaagccct accacctttg aacaacggga tacagcgacg cctaacaaca
     6421 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     6481 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     6541 tagacaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggc
     6601 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     6661 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     6721 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     6781 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     6841 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     6901 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     6961 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     7021 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     7081 tagaaatccc gatcatttta cgcgatcgat gggcatataa ataaaagaag agttgtagtc
     7141 ctaaatgagg attgcagctc tttttgtttt acatagaaac aggaaaagac ctttttagga
     7201 agacacaagt gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc accgccgttc
     7261 gagaagaaag gaagtgaacg ctgcacgttc actaagatat ggtgtcttgc aaagcaagac
     7321 aaaatagaca aaacttcctc tcaccaaaag gttcgttcct gttcgttatc tctttattga
     7381 tttgccagac cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga caagggtaaa
     7441 tggcacgctg cgcgcccttg tccttgtcgc ttttcgttga tttattctgg ccaacaaatc
     7501 aataaagaaa gaactaacga aaggaagtaa aacctttcct aagaggaggc agaatcaaac
     7561 atgcgaaaga aatttgatta ttggggtgtt cccttcagcg aattattccc aaactatcat
     7621 gcaccacata ctgttgaatg cgattgtgga gaaagagcga aatgtatcaa aagctatcgt
     7681 ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt cttatggcga ttacattttg
     7741 attgatgaga aaggaaagaa acgctgatga aaacacaaca caaattattg actgtaaaag
     7801 aagtcgttca aattttggtg gacaaagaag tcatttctga aaaagtagca gaagctttac
     7861 ttattccgcg gtactggacg atagaaaccc ctttagtaga ggatgcaccg actatgctat
     7921 ttgaagacct agtgaagatt gcaggatatg aactgcatta tcaagaaaac atggaaactt
     7981 atgaaatcga atattggtta accaaattag agaactaaaa aggagaaaaa atatggctca
     8041 aaattactat gacgaatttg tcaaattacc tttggataaa atggcacaaa aaatggagga
     8101 tatgacgttc ctataccatg aaacacgtgt acccaaaaaa cattacaagg aaaaattgtc
     8161 agtcgcagtg gaagaaatga ttgaatcagg tgtcgaaatg aatctcattg cgacgtacta
     8221 tcgaacactg gaagaactga aaaaacaaaa tggaaaatgg tttttccaag ccttactatg
     8281 cttggaagta ggtgtcaaac caagcactat caaaccttcc gaatatcaag ccttagaact
     8341 gacttatgca aaattcgcac agattaagaa agctaaagca atggctagcg actggctaga
     8401 tacttttgaa agtatcgaaa aaaatggtgc attttatacg attgaatcgg aggagaaaga
     8461 taatgaataa ggtggtttat cagctaacaa cagaagatgt ttcgcaattt ttaacaagca
     8521 aagaacactt gaatgaagta atcagacgga ttcaaaaaga ctttagttgt atggaagaaa
     8581 tgaagtactt tattcaaaga gtggaagaac agttgtatat ggaaagcgaa acaagtcaaa
     8641 cgaaggtcac agtcagccat aatcatttag acgtgtataa acgtcgtcct gaaacggtaa
     8701 aaaaaggaac agagcttcta cgactagaaa cagaaaaata catcatgaat gtgtacctag
     8761 aaaggagtga gctggtcgaa aactttgtca gctacacgat ttgtaggaaa cactattact
     8821 cagaatcaga atattattca attttcgaga gcatggaatt aatggaagaa gaaatggaag
     8881 agctaaacag caaaaatgcg acggtagacg attattacct tgtatttact aggtttttga
     8941 cgtgtgggac ggaatctatc gtgttaatgg gaatggatta tgaactcgct gtacccgaag
     9001 catttttgag ggaatatgta gaagaagtag ataacccagg cgacgactgg tacacgactt
     9061 taactgattt tttagacacc tacacttacg acgatacaga agaaatccaa aatgaacttg
     9121 accgtaaacg aatgccatat caacgatttg aaaggaaaaa aggtaaataa ccatttatac
     9181 cgaaaagtga ggatgtgtga aataagaaca aaaaaccttg aaattaaggg aatggttctt
     9241 atttcgcacc aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat aagcgggcaa
     9301 agcccgataa cctaagagaa agaaggagat ccatatgaat aaaatcattc ctctattgtt
     9361 cgtactgttt gttggcgaga tacttatgcg tcatgctgca gctgtacatt taaacttcgt
     9421 agtattctta ggaatactgg tattttgttt gatcttttgg cgatttgtat tagcccttgt
     9481 gagtctacta ttcaagatga ttgcagccat atttttcata gggttatttc gatgaaagga
     9541 gagagtacta atgccaattg caataggaaa taaacggtta cccgtgacat tagatgaaaa
     9601 gagacaaaaa gaattgcagc aactaaagca gaagtacgga aaaagtgaat ctaagattat
     9661 gtgtattgcg ttagatctat tgatcgcgca agaaaaagca ggatttgata taccagcact
     9721 aaaaaagtga cgtcactttt catttgaaga aacaaaagtg atagcactta attgttccta
     9781 ctatttagaa tattttctat gtccttttta ttccattttg tatcaataac tatgaaatct
     9841 ttaaagtatc cagaagagat ta
//
LOCUS       DAXWLH010000098         9727 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXWLH010000098 DAXWLH010000000
VERSION     DAXWLH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.099"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            254..733
                     /locus_tag="ACGE36_001876"
     CDS             254..733
                     /locus_tag="ACGE36_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFF8384258.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACGE36_001877"
     CDS             766..2994
                     /locus_tag="ACGE36_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8384259.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACGE36_001878"
     CDS             3123..4766
                     /locus_tag="ACGE36_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8384260.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACGE36_001879"
     CDS             complement(4839..5384)
                     /locus_tag="ACGE36_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFF8384261.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACGE36_001880"
     CDS             complement(5407..5892)
                     /locus_tag="ACGE36_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8384262.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACGE36_001881"
     CDS             complement(5882..6694)
                     /locus_tag="ACGE36_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFF8384263.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACGE36_001882"
     CDS             complement(6833..8365)
                     /locus_tag="ACGE36_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFF8384264.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACGE36_001883"
     CDS             complement(8474..9505)
                     /locus_tag="ACGE36_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8384265.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3248 a         1817 c         1874 g         2788 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagca
//
LOCUS       DAXWLH010000099         9665 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXWLH010000099 DAXWLH010000000
VERSION     DAXWLH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.100"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            220..1191
                     /locus_tag="ACGE36_001884"
     CDS             220..1191
                     /locus_tag="ACGE36_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFF8384266.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1215..3407
                     /locus_tag="ACGE36_001885"
     CDS             1215..3407
                     /locus_tag="ACGE36_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFF8384267.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            3424..3900
                     /gene="ybeY"
                     /locus_tag="ACGE36_001886"
     CDS             3424..3900
                     /gene="ybeY"
                     /locus_tag="ACGE36_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFF8384268.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            3884..4279
                     /locus_tag="ACGE36_001887"
     CDS             3884..4279
                     /locus_tag="ACGE36_001887"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8384269.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            4294..5193
                     /gene="era"
                     /locus_tag="ACGE36_001888"
     CDS             4294..5193
                     /gene="era"
                     /locus_tag="ACGE36_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFF8384270.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            5336..6148
                     /gene="recO"
                     /locus_tag="ACGE36_001889"
     CDS             5336..6148
                     /gene="recO"
                     /locus_tag="ACGE36_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFF8384271.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            6532..7449
                     /gene="glyQ"
                     /locus_tag="ACGE36_001890"
     CDS             6532..7449
                     /gene="glyQ"
                     /locus_tag="ACGE36_001890"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFF8384272.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            7451..9526
                     /gene="glyS"
                     /locus_tag="ACGE36_001891"
     CDS             7451..9526
                     /gene="glyS"
                     /locus_tag="ACGE36_001891"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFF8384273.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         3240 a         1742 c         2082 g         2601 t
ORIGIN      
        1 ttagacggaa tacttgtcct ctatcttatg catagcttac atgatcagct ttttttattc
       61 aaacgagacc tttagagaat ggaagcggct agttttttaa cgtttcttgg tacttatggt
      121 taagcaactc ttttagaacc ttttcttatt tgctatatag tttcttctga gtatggtaaa
      181 ataggaaaga atgaatcgaa aaaaagggga tgtctatttt tgacggaaga gtctagttct
      241 ttagagatta gattgacggc tgctgatgat gtcagcatgc ttttaggtgc acacgacaag
      301 catataaaac tgattgagga aacaacggaa acaaccatcc atacacgtgg agaaatcatt
      361 cagatcattg gtgagcaatc ccagatttcc ttggctgcct cagtcattcg gacattacag
      421 gaattgatca aacgaggaat ccacgtaagt acgcccgatg ttgtgacggc attgaaaatg
      481 gggcaaaaag gtacattaga ttattttgtt gagatgtatg aggaagagat cgtcaaagac
      541 cgtaatggaa aacctatacg ggtaaaaaac agcggccaaa agaaatatgt cgaatcggta
      601 gccaaacatg atatcgtatt tggcgtagga ccagccggta caggaaaaac ttttcttgca
      661 gtagtattag caatcgctgc cttgaaaaaa ggtgaagtgc aaaaaatcat tttgacgcgg
      721 cctgctgtgg aagcaggtga aaatctaggc tttcttcccg gggatctaaa agaaaaagtg
      781 gatccttatc tgcggcctgt ttatgatgct ttataccaaa tttttggatt ggatcatacg
      841 aaccgattga tggaacgagg cgtaatcgaa atcgcacctt tagcttacat gcgggggcga
      901 acattggatg atgcattcgt tatcttggac gaagcacaaa acacgacagt cgctcagatg
      961 aaaatgttct tgacccgtct cggttatcaa tcaaaaatga ttgtcaatgg ggatacaagt
     1021 cagatagact tgcctcgagg gacgacaagt ggtcttgttc atgcagaacg aacattaaag
     1081 caaatcaaga aaatcgattt tgttaatttt gaagcaagtg acgttgttcg gcaccctgta
     1141 gttgccgaaa tcatcaaagc ttatgaaaag gcagatctcc atcaagaata agctttgctt
     1201 aggaggataa gcacatgttg aataaaacac tgcagaaact acaagaaaaa cttggaaagg
     1261 cagcattgcc tttcctgctg ttcttgtttt ttatcattat ctgcggcgtt acatttagta
     1321 gtgtcaaaca aaaggccaat gatttcagag aaggccaagt agcagaagaa agtatccgtg
     1381 cgaacaaaac gatcgagaac acggaagaaa ccgaacaaaa aagaaaactg gcagcagaag
     1441 cagtcactcc agaatacact taccaaaaag atcttgcaga tgaccagaac aatcggatca
     1501 agcaactatt tgaattgatc gataagacga atgatgccat caacaaatct tatgacgaaa
     1561 agattgataa ggcaaaggaa gatgaaaata tcccaaagcc tactacagaa gagcggatcg
     1621 ccgcgctgaa gaaaaatttt gaaaatgtga atgcagaaaa tgttgtgttt tatcaaaaac
     1681 taccgaacaa tttttattcg acgatatttg aaatgactga aactgagatt catactgtga
     1741 gggatgagag tctgaagctc attgacgaac agatgagcaa acaagttcgt gaaagtgaat
     1801 tggaagcttt taaacaagaa gcagaagagc aaatccaata tttgaatgtc acaccagcac
     1861 aacaacaaat gattcgctac ttagtggatc aaggtatcgt agtgaatgac gtattgaatg
     1921 agaaaaagac agaagaattg aaacaatccg cacgagaagc tgtacaacca gtgatgatct
     1981 tccaaggaga aatcattgtt cgtgaaggta atcaaatcga tgcttcagct atgaaaaaat
     2041 tggaattact aggactgact aatcaaacaa cttcgatttt tccgctaatt gccatggttt
     2101 tagctgtttt gcttcaaatc ggcgtactga tttataattc acttcaattc caaaatgcag
     2161 gagaaagaac aaaatacgtg cttttctatg tgacagctat gtcagtcagc atagtgctga
     2221 tgaaattttt ccaattattc caaacggaac aagctactta tatcccgctg ttttatcccg
     2281 ctgcttttgc accacttgtt ttaagctttt tcttgaatcg aagagcaggg atcatggctg
     2341 gtattttcca agcagtatcc gcacttttca ttttttatga ttcgattggc acgaactttc
     2401 taacgattat cttgatgtct tatgttttct ctgggctcat ggcaacgatt gtcagacgca
     2461 aacggatctc agaacaagga ttatctgctg ccatgtgggt cattattttt cctgtgatga
     2521 tggatgtcat tttgatcgtc tatcaaggaa tgagctttgg tgatgcaacg acttggttaa
     2581 tgctcgtctg cgggttcact ggtgcactct tctcattctt gctgacgatc ggtctacacc
     2641 catatattga attgatggtc aatgatgaca gtgtaattgt attaaatgaa ctcagcaatc
     2701 caaatcatcc tttattgaaa cagttgctgg aagaagcgcc aggtacttat catcatagta
     2761 tgatggtagc taatctaagt gctaacgctg tagcagaaat cggcggacgt tctttgctaa
     2821 ctcgagtagc ttgctattat catgatatcg gcaagatcaa acatgctagc tttttcgttg
     2881 aaaacttgcc ttcaggagcg gaaaacccgc acaatttctt attgccagaa gacagtaaac
     2941 atattatctt tggacatgtg acagatggag ccaaaatatt ggaagattat aatatgccgc
     3001 aaatggtgat cgatatttgc aaacagcatc atggaaccac gctaatgcgt tatttctaca
     3061 tcaaagcgaa agaacgcaat ccggaagtaa cagaagagca attccgttat cctggaccaa
     3121 aaccgcaaag tcgtgaagca gggatcgtca atattgctga cagttgtgag gctgcagtac
     3181 gggcaatgga tcatccgacg agcgaaaaaa tcgaaagttt tgtccataat cttatcgaag
     3241 agcgtatttc cgatggtcaa ttagacgata gtggactgac cttgaaagaa atccgaaaag
     3301 tggaaaaatc attaatcagc ggtttaagta gcaccttcca ttctcggatc aaatatccta
     3361 aaatgaaaac agaagttgag aagataaagg aagaacaaga agaatagaaa gaagcgatgt
     3421 ataatggata ttacctttat ggatgaaaca aaccaagtct cagaagagaa aatgaaagaa
     3481 atcgatgact tgctgcaatt tgcagcatct tatttaaagc tgccagagga aacagaaatg
     3541 tctgttactt tcatggacaa tgcggcaatc caagtaatca atcgtgatta ccgtggaaaa
     3601 gatgcaccga cagacgtcat cagttttgct ctggaagaag aaggcgaaga cgaaatccca
     3661 gtgatttttg aagatggaga gaatccatta cctcgtgtac taggagacat catgatctct
     3721 attgaacgtg ctgctgaaca agcagaagct tacggccatt catatgatcg ggaacttggc
     3781 tttttagcgg tacatggatt cttgcatatc aacgggtatg accatatgac accagaagat
     3841 gagaaagaaa tgtttggttt acagaaagag atcttggatg cctatggact caaaagataa
     3901 aaacgtagag aaaaacaaac atttcctgat gtctgtggaa tttgctgtcc aaggagtcaa
     3961 aaccgttttt gaggaagaac gcaatatgag gaaacatgtt gcgttcggta tccttgcttt
     4021 atttatgggc gtgattttcc agcttgatcg gatagagtgg ttatggctgc ttttagctgt
     4081 ttttctcgtt tggatagtag agatcatcaa tactgttttt gaaaatgtag tggacatgtt
     4141 tacggatttt cattttcatc cgattgggaa aaaaatcaaa gatatggcag ctggagcagt
     4201 tttagtgacc gcttgttttg ctgtcatcat tgggttgatt ttatttgtcc ctaaaatata
     4261 tcatttgttg tttaattaaa aaggagaacg tatatgccag aacataaatc agggtttgtc
     4321 gcaatcgtcg gccgcccgaa tgtaggaaaa tccacattat tgaaccgaat cgtgggtcaa
     4381 aaaattgcca ttatgagtga caaggcacag acaactagaa ataaaatcca aggagtatat
     4441 actacaccag aagctcagct gatttttatc gatacaccag gaatccataa accaaagcat
     4501 cgtctaggag attttatggt agaagcagct tacagtgcgt tgaaagaagt ggatgcagtc
     4561 ttatttatga tcagtgctga tcaaaaacgt ggccggggag acgattttat tattgaacgt
     4621 ctaaaaaacg tgcattctcc tgtttatctg attatcaata aaatcgataa gatccatcca
     4681 gacgagttgc tgggaatcat cgaagattat tcgacgcaga tgccttttgc acaagtgatt
     4741 ccgatctctg ctacagaagg aaacaatgtc gaacgcttaa tggatgtatt agtcagtgaa
     4801 atgccagagg gcccacaata ttttccagat gatcaagtga ctgatcatcc tgaatacttc
     4861 atcgtttctg aattgatccg tgagaaagtc ttgtttttaa cacgagacga agtgcctcat
     4921 tctgtcgcag tcgttgttga ttcgatgaag cgaaacgaga atgataaaat ccatatccag
     4981 gccacgatca tcgtcgaaag agatagccaa aaaggaatca ttatcggcaa aggcggaaaa
     5041 atgctgaagc aagtaggaac gaaagcaaga aaggatatcg aaaatctttt aggagacaaa
     5101 gtatttctag aactttgggt caaagtccaa aaagactggc gagacaaaaa agtctatctg
     5161 caagacttcg gctaccgaaa agacgagtac tgatattctg gacttcttat aggttaggta
     5221 tagaaataaa ctggataaaa atccgtacgt tgagaaaaac agcggaagat cgaaacataa
     5281 ataatctgga tcggcagaat ctgcttttcc agatttattt tttaggaggg agaatatgag
     5341 ccagcttgca gaatcgaaag gcttgatttt atctagccgt aattacaaag aaaaagataa
     5401 attagtcaaa atctttacgg aatcttctgg caaaatgatg ttttatatca aaggaatcca
     5461 tcggcaaaat cagccattag ctccggcttt gctccccttt accgaagcag tctatatcgg
     5521 taattttcga agtgaaggtt tgtcgttttt aagcagtgtc aaagacgtcc atgcgtttcg
     5581 gaacatccaa gaagatatct ttttgagcgg ttatggtacg tatttgctaa atctagtaga
     5641 tgcagcaatc gaagatcatc aatatgaccc gcatttattc caatttacgc aacaagctct
     5701 gcagcggatg gaccaaggag atgatgcaga aatcatcacg aatatctttg aagtgcaaat
     5761 actgcaacgc ttcggaatcg cgccaatttg gacgcactgt gtcgtatgcg gggaaacaaa
     5821 aggaaaattt gactattctt ccaaatatgg cggtgtgata tgcgagaaac actggccaat
     5881 ggacgaacac cgttatcatg cagatcctcg cgctgtttat ttcgttcgaa tgttttcggc
     5941 tatttcttat gataagatca gtggaatcca cttgaaagaa gaaacgaagc aggcaatccg
     6001 tgacctcatt gaccagttgt atgatgaata tgtcggtatt catctaaaaa gcaaaaagtt
     6061 cattgatcaa atgaagtctt gggcacacgt attaaagacg ccagagaaaa atatgcctga
     6121 aaaagagata cctgaaaaag aagaataaat gaaagataca acaaaaaaat caaaactatt
     6181 tgacaaatac agggacttcc gtctattatt aaccgtagat taaatagatc gttcactgag
     6241 aggatctatt atacaaacca gaaaactgca aaatgaatac gtacattgac caagatcagt
     6301 aaaaaacacg tggtttagcg agttcgggag agtggaagcc ggacccaaac gatttttgaa
     6361 gaggcacttg cgagtacgat aaatgaagct gccgaatctt tattcggaag tagggtggaa
     6421 ccgcgaaatt attcgtccct atgtctgtaa tcagacatag ggactttttg tcgttttctt
     6481 tcctgtattc ttattgacag caacgcttaa aaattgaaag gggcaaaaca gatgagtaaa
     6541 aaattgaccg tccaagatat gattttaaca ttacaaaaat tttggtcatc aaacggctgc
     6601 atgctgatgc aagcttatga tacagaaaaa ggagcaggta cgatgagccc atatacattc
     6661 ttacgggcaa tcggaccgga accatggaat gctgcttatg tagagccatc ccgccgacca
     6721 gctgacgggc gttatggaga aaacccaaac cgtttgtatc agcatcatca atttcaagtc
     6781 gtaatgaaac cttcacctga aaatatccaa gagctttatt tagaaagctt gcgtctttta
     6841 ggaattgatc cattagaaca cgatattcgc tttgttgaag acaactggga gaacccatcg
     6901 atgggttgtg ccggtgtggg ctgggaagta tggcttgatg ggatggagat cacacaattc
     6961 acatacttcc agcaagtcgg aggactacct tgtaaaccag ttacttctga gatcacttat
     7021 ggactagaac gtcttgcttc ttatatccaa gaagttgaaa gtgtttatga tctggaatgg
     7081 gctgatggcg tgaaatatgg tgagatcttc aaacaaccag aatatgaaca ttctaaatat
     7141 tcttttgaag taagcgacca agacctgcta ctaagcaatt ttgatcgttt tgaaaaagaa
     7201 gctaaacgct gtatcgatga aaatcttgtt catccagcgt atgattatat tttgaaatgt
     7261 agccatacat tcaatttatt agatgctcgc ggtgcagtat ccgtaacaga gcgtgcaggt
     7321 tatctttccc gtatccgcaa catggctcgt gctgtagcca aaatctttgt ggcagaacgc
     7381 gaaaaattag gattcccttt attgaataaa aacacagcaa atccagaaaa cgtgaaggag
     7441 gctgaataag atggcaaaag acttattact tgaaatcggg ctggaagaaa tacctgctca
     7501 tgtagtcaca ccaagcatga aacaattaga agaaaaaaca gcaaaatttt tagatgaaaa
     7561 ccaattgact tatgatacga tcgaaacgtt ttctacaccg cgccgcttag caatcaaagt
     7621 aacaaacatt ccagaacgtc aagcagacag cgaagaagaa gtcaaaggac cagccaaaaa
     7681 aattgctttg gacgcagatg gaaactggac gaaagcagct gaaggatttg tccgcggaca
     7741 aggtctgacg acagatgcta tcacttttag agaattaaaa ggcgtggaat atgtctatgt
     7801 aacgaaatac actcacggca aagaagcaaa agaagtattg acaggtttag cagatgtcat
     7861 taaaagcctc actttcccag taacgatgca ctgggcagaa tacgattttg aatatattcg
     7921 ccctattcac tggatcgtgg cattgttaga tgacgaaata atcccgtttg agatcttgga
     7981 tgtcaaaaca gacagagctt caagaggtca tcgtttctta ggcgaagatg tgacatttac
     8041 gcatgcaaaa caatacgaag aaaaattgaa tgaacaattt gttattgctt caccgcaaaa
     8101 aagaaaagaa atgatcatca cacaagcaaa cgaattagca gaaaaaaatc actggatcat
     8161 tgatctagat gaagatttac tagaagaagt caacaattta gtcgaatatc caactgtctt
     8221 ttctggcgac ttcgaagaaa aatatctatc tgttcctgaa gaagtactag tcacttctat
     8281 gaaagaacat caacgttatt tcgaagtacg gaacgatcaa ggtatgttgc ttcctcattt
     8341 tatttctgct cgtaatggcg atcgggtaaa attggaaaat gtggtacgag gcaatcaaaa
     8401 agtattgact gctcgtttag aagatgcgga attcttttat aatgaagaca agaaagcttc
     8461 aatcgaccaa tacgttgaaa aattaaaaac tgttactttc catgaaaaaa tcggtagcat
     8521 ctatgagaaa atgcaacggg tacaagtcat cgctcgcatc ataggcgaac aagtaggact
     8581 atctgaggaa gaattgatgg atctgcaacg cgctgctgaa atctataaat ttgatctagt
     8641 aaccaacatg gtaggagaat tccctgaact acaagggatc atgggcgaaa aatatgctct
     8701 gcttaaaggc gagaaaccag ccgtagcaac agcgatccgt gaacattaca tgcctatatc
     8761 cagtgaagga gatttacctg agactacagt aggttctgtt ttagcgattg ctgataaatt
     8821 agatagtatc ttttctttct ttgcagttgg gatgatccca agtggctcaa acgatccata
     8881 tgctttgcgc cgtcaagcat atggtgtcgt tcgaatcatc gaatcgaaaa aatgggcctt
     8941 tcctttgtct gttctgcaag aaacgattag tgaagttatc agtaaagata cggatcgttt
     9001 tggcattggc ttgtcagcgg gacaacaaca ggtgatcgac tttatcaaag gccgtctgcg
     9061 ccaattattg acaacgaaga atatccgtca tgatgtcatc gaagctgttt tgaatgcaga
     9121 acaaaaagat ctgacaaaag tatttgcagc agcacaattg ttcaagcagc atcttgcaga
     9181 tgaagacttc aaaccatcga tggaagcttt gacacgtgtc gtgaacttag cgaaaaaagc
     9241 ggagcttgaa aaacaatcag aagtagaccc tgaactgttc gaaaacgaag cggagaaaga
     9301 actgcacaaa gcagtagagg caatgaaaac aagctttgct gaccaaacga tcaacgaaaa
     9361 ttatgatcag cttgtaagcc ttcgcccact gatcgaaaat tattttgatc agacgatggt
     9421 catggcagaa gaaccagcag tacgtaagaa ccgattaacg caattaagtc aaattgctgc
     9481 catggctttg tcactagcaa gtttagatca aatcatcaca aaataatttc taatatgtaa
     9541 ataaaccaga gaacaggcac aaaataggct atttctctgg tttatttgtt ataaatataa
     9601 ataactatta ttaattcaat gttaattttt ttgttatttt attttttgtg taacttttaa
     9661 aaaca
//
LOCUS       DAXWLH010000100         9663 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXWLH010000100 DAXWLH010000000
VERSION     DAXWLH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.101"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            33..656
                     /locus_tag="ACGE36_001892"
     CDS             33..656
                     /locus_tag="ACGE36_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384274.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACGE36_001893"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACGE36_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8384275.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACGE36_001894"
     CDS             2152..3033
                     /locus_tag="ACGE36_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384276.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACGE36_001895"
     CDS             3036..3806
                     /locus_tag="ACGE36_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384277.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACGE36_001896"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACGE36_001896"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8384278.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACGE36_001897"
     CDS             5117..6280
                     /locus_tag="ACGE36_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8384279.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACGE36_001898"
     CDS             6397..6807
                     /locus_tag="ACGE36_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8384280.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACGE36_001899"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACGE36_001899"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8384281.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSNLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..>9663
                     /locus_tag="ACGE36_001900"
     CDS             9380..>9663
                     /locus_tag="ACGE36_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384282.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3356 a         1639 c         2077 g         2591 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctaatt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 tta
//
LOCUS       DAXWLH010000101         9595 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXWLH010000101 DAXWLH010000000
VERSION     DAXWLH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.102"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            73..3648
                     /locus_tag="ACGE36_001901"
     CDS             73..3648
                     /locus_tag="ACGE36_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8384283.1"
                     /translation="MISNKKRWLKVALLVAFCQIAFLASMTTQAEEIVQETTVSQVSP
                     TSNEVEVQSTLPKEAVKEETPSEVVPETEAPSTNEKTVLDETIGKSQQPGDSPVVAEE
                     TPVEAHEAQAIPEKDKSLEESGSTEINEGTEPVQLEDSKEVTTPIESTKEEMDSNTSL
                     SSEKEEKTVLLTEPREPELKKVEEFVDTKKEAVSPTSSKEDTLIVQQTARSARVRRAT
                     NTAVLGGRKIDWGENIGVISSANNPNFTLNYMVRFEINGQVAFCIEPGVAAAEGQVYT
                     ATALENYLKDLNIRKKTSLISYLGYVTSKDKSDEQYIAAQFMIWEVLGTKIPIKNIRI
                     DYDRRKAEIQRLLNNFDVRATFHNQTHTVKIGETLKVKSGNDFLQRVKDIQKPKGVNV
                     SIHDNEVWITITKESPEKAKIHFNQIEALGVPLAYRRPGAQTIGVLNPFVPGDSVVNL
                     TILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQANGKKIKEITLKNINGKVQAEI
                     KDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNTIAKDQVIKGTIDLVKVANKD
                     LVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTVVFDTYILSETK
                     GKEGYKLFEPFEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKVIALAGTQFKIW
                     DKYANNGKGGYVSMALPNDTEVSDIFTTNEKGYFVTTDTLKYGKNRYELREIKAPEKY
                     VLNKTPYVFSVTDDTNTIQLIYFANRLAKANVEVFKYDTSTNFDQKTAIAGVEFDLFK
                     KDETGTTFVGTFKTDKKGKINVKDLLVGDYFFKEVKPLAGYLALDKDVPFTVTVDKDG
                     TVIALEVENKRIPPKIGTTAIGKYTNQQKVTPLKKVTITDTVKYSNLIIGKTYTVNGV
                     LMDKEESITAGKDVPLLVNGKQITASKVFVAKNPNGMISLDFVFDASALAGKEIVVFE
                     NVYRDGRLVASHTDIHDKGQTVRIVNPMIHTKATGQNGEKQLQALPNQKVKELVQLND
                     LIVGQTYKVLVQGYITPDGVKLDGTSAEKIFKADKTTLEFLFEFLVDGRKLAGKGLSF
                     AEWLEAQSEPKDENDYEVRFEQIANHNVDLKSEDQTVYFVEVPKVPEPKIPEKSQPFK
                     EVKQASVVPMLPKTGSEDTVLWMIIGVLFVLAAGLTFYELRKVKD"
     gene            3748..4686
                     /locus_tag="ACGE36_001902"
     CDS             3748..4686
                     /locus_tag="ACGE36_001902"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8384284.1"
                     /translation="MKKKERKKRMKKLFFACLFSSVVLAGGINVFADEIGITPVTPPV
                     IEETTPVVPIPDISKIDESVPDQPTPVDPDISSEVTEPTTPVVPDITENSENETPVVS
                     PSTGETTNDKDTTPTTSESYSGTSSVDNDSKIDDFSSSSTTIDTPKETEKKNDLGAPK
                     NLTPENHIKTVSEKEMTVSVSSDGKIVQGTGTEKSIPIVTSNLEEINHVPTSETPLQV
                     ATGQAIVGVQDGVPLVQDNQGNLVEDLSIPVKKLPSGNIEIKTIDGKTKVLPKTGEEL
                     QISLTILGSLLTAVTGGIFYGKKQWRHLRHMFKKTI"
     gene            4705..5055
                     /locus_tag="ACGE36_001903"
     CDS             4705..5055
                     /locus_tag="ACGE36_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384285.1"
                     /translation="MGLSYVAISQEEVLGKCFNQKHVGSTGERDRRGNMKKVEWEIFQ
                     MNSEKMGEIEVRVAVGGAPKAMRYNAEVLLANPRISRRADVRNIGNDGETNKIARNRF
                     YLLVDRVEVKGGNN"
     gene            5055..5486
                     /locus_tag="ACGE36_001904"
     CDS             5055..5486
                     /locus_tag="ACGE36_001904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFF8384286.1"
                     /translation="MTLEFDQGIVPGLVKETLGEIYFVKAQRPRMLYEDNENTGEIKE
                     RPVEFSSATQETMFVVNFPPKTNLDGLSFGDVVELEEVEFRFWISIDRESMSNRAEND
                     LKVSALRYKKTGKNVFQTSSSTRATYVKEKQDSNKESAKKE"
     gene            5754..7073
                     /locus_tag="ACGE36_001905"
     CDS             5754..7073
                     /locus_tag="ACGE36_001905"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8384287.1"
                     /translation="MFQYKGIRIRNRHKYASERFIFYLMLPVLLLMSGFFSLFYTTIT
                     WEWCCIYWIIAFGLAGLFAYMCLYFYFYNVYQRLVHKGKLAKMIHSRRLILERVLKNG
                     RRKVVYYPKIFYHYKNGLIHVRLPLDLGPFQDRFNEMAGQLEEAFFCDLVEEQREKNY
                     ICYTFLYDVEKNRIGIRDITVKDGIITLMKNVQWAFNKAPHAIIIGSTGGGKTYFIMS
                     LIYALLPYGTFDICDPKRADLASLEKIPIFKDQVFYGGGILSCLINLAKETANRYDFL
                     REQPEYELGYDYTYYHLTPHFLVIDEWAAYYASLDTKEKKKAMGCLNQIALMGRQCGV
                     FLILGTQRPDTKYLDGAIRDNFGLRVTLGRVSKAGYKMIFEHTVKSLFNKPIKGRGYI
                     DSGTDQVREFYAPFIDITRFNFFAEFKQLSDQLMAEKIKETKEGKNV"
     gene            7066..7230
                     /locus_tag="ACGE36_001906"
     CDS             7066..7230
                     /locus_tag="ACGE36_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384288.1"
                     /translation="MYEEIPATCGTRLEIKDLMIDSFLAIVFSEKRLSFFINKKVENR
                     WRLERMYPTS"
     gene            7769..8944
                     /locus_tag="ACGE36_001907"
     CDS             7769..8944
                     /locus_tag="ACGE36_001907"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8384289.1"
                     /translation="MDGTQLKQMRLSRNLTQKLVADTLGWPQSFLSMIENDQRSCSKE
                     QEEALCEFYANFSSFTSLEIKFDYVRVRVPTNDTKTVVEEILGMDFNSFFYAETRLYG
                     YIEMYQKGMIRVLNSKKGDDKGVLIELSGQGCRNYESVLDERNDSWYQFFTRCVSFNG
                     VPRRIDIAVDDFEELFSLHEFAQKLNRGEFDSKFRTWRFQEEKQLLDNLSSGLSVYLG
                     SSQSLMYFCFYQKNYEIAKKQKLPLEEVEVKNRYEIRLRDAKAVSFIEQYLNKVDFSD
                     LTMGILANQLIMFEGEKNANVWKPWGKLIQNVGKVNLTITPEKPNYNRKLMYLKTQPA
                     RTMKIVKTVDEILGLNRFGEILEETELREQDEKIVEDEVRAVYEYIQENPDAIHYLL"
BASE COUNT         3433 a         1385 c         1971 g         2806 t
ORIGIN      
        1 atgtcttatt tccatccata tagtctattt caaacagaag agaacaatta aaaagaaagg
       61 agatttcttg ttatgataag taacaaaaaa cgttggttaa aagttgcttt actggtagct
      121 ttttgtcaaa ttgctttctt ggcttcaatg acaacacaag cggaagaaat agttcaagaa
      181 acaactgttt ctcaagtttc tcctacttca aatgaagtag aagtgcaatc tactttacca
      241 aaagaggccg ttaaagaaga gacaccatca gaagttgttc cagagaccga agcaccttca
      301 acgaacgaaa aaactgtact cgatgaaact atcgggaaaa gccaacaacc aggagatagt
      361 ccagttgtgg cagaagaaac cccagtagaa gcacatgaag ctcaagcaat tcctgaaaag
      421 gacaaatcat tagaagaaag tggttctact gaaataaacg aaggaacaga accggttcaa
      481 ttagaagatt caaaggaggt aacgactccg attgaatcaa cgaaagaaga aatggattct
      541 aatacttctc tttcttctga gaaagaagag aaaacagttc ttttgacaga acctcgtgaa
      601 cctgaattga aaaaagtcga ggaatttgtt gatacaaaaa aagaagctgt ttctcctacc
      661 tcttcaaaag aagatacatt gattgtacaa caaaccgctc gaagtgcacg ggtgcgacgt
      721 gcaaccaata cagcagtttt gggtggtcgg aaaattgatt ggggcgaaaa tattggtgtt
      781 atttctagtg ccaataatcc caatttcact ttaaattaca tggttcgttt tgagattaat
      841 ggacaagtag cattttgtat tgaacctggt gtcgcagccg cagaaggtca agtttataca
      901 gctactgctt tagaaaacta tttgaaagat ttaaatatac gaaaaaaaac aagcttgatc
      961 tcttatttag gatatgtcac aagcaaagac aaatccgatg aacaatacat tgcggcacaa
     1021 tttatgattt gggaagtttt gggcacaaaa attccaatta aaaatatccg tattgactat
     1081 gatcgtcgaa aagcagaaat tcaacgtttg cttaataatt ttgatgtaag agctactttt
     1141 cataatcaaa cacatacggt aaaaattggc gaaacgttaa aagtaaaaag tggcaatgac
     1201 tttctacaac gtgtaaaaga cattcaaaag ccaaaaggtg ttaatgtatc tattcatgat
     1261 aatgaagtat ggattacaat cacaaaagag tcaccagaaa aggcaaaaat tcatttcaat
     1321 caaattgaag cattaggcgt gccattagcg tatcgtcgtc caggtgctca aacaattggg
     1381 gtgctgaatc cttttgtacc tggtgactct gttgtcaatt tgaccatttt gaaacaagga
     1441 aacattcgtg tctttaaaga agatgcagaa accggtgtga aagcacaagg tgcagcttct
     1501 ttagaaaatg cagtgtatgg cttgtatcaa gcaaatggga agaaaatcaa agaaattaca
     1561 ttgaaaaata tcaatggcaa agtacaggca gaaatcaaag atcttgatcc tggtaaatat
     1621 gttttaaaag agatcaaagc accaaaaggt tatgtacttt ctgatgaagc aattgaaatc
     1681 gtcgttgaac ctggaaaaac tgtcaatacg atcgcaaaag atcaagtgat caaaggaacc
     1741 attgatttag tcaaagtcgc aaataaagat ttagtcgatt caaccaatcc agacaacaaa
     1801 cccaaattag ctggtattga aatctctttg acgtcaaaaa caactggaaa agtggtgaaa
     1861 acagttgtga cagataatga tggttacgct acttttggta aaaataccgt tgtctttgat
     1921 acgtacattc tttctgaaac caaaggaaaa gaaggatata aactatttga accattcgaa
     1981 gtcacaattt ctgaaaatgg acaaaccttc cattatgttt tagaagacaa agtaatcgaa
     2041 caacgtctta aagtggtgaa agtggatcaa gaaacaggga aagtcattgc tctagctggt
     2101 acccaattca aaatttggga taaatacgca aacaatggta aaggtggcta tgtatcgatg
     2161 gcattaccga atgatacaga ggtttcagac atatttacaa caaacgagaa agggtacttt
     2221 gtcacaacgg atacattgaa atacggaaaa aatcgttacg agttgcgaga aatcaaagca
     2281 cctgaaaaat atgttctaaa taaaacgcct tatgtctttt cagtaacaga cgacaccaat
     2341 acgattcaat taatctactt cgctaaccgt ttagcgaaag ccaacgtaga ggttttcaaa
     2401 tatgatacta gtacgaattt tgatcaaaaa acggcgattg ctggtgttga atttgaccta
     2461 ttcaaaaaag atgaaacagg cacaaccttt gttggtacat tcaaaacaga caaaaaaggt
     2521 aaaatcaatg tgaaagactt attggttggc gattacttct ttaaagaagt gaaaccatta
     2581 gcagggtatc tggccttaga taaagacgta ccatttactg taacggttga taaagacggt
     2641 acagtgattg cactagaagt ggaaaacaaa cggattccac ctaaaattgg tactacagct
     2701 atcggtaagt atacaaacca acaaaaagtg accccattga aaaaagtaac gattacagac
     2761 actgtaaaat acagtaatct aatcatcgga aaaacttata ccgtcaatgg tgttttgatg
     2821 gataaggaag aatctatcac agcaggaaaa gacgtaccgt tgttagtaaa tggtaaacaa
     2881 attaccgcaa gtaaagtgtt tgtcgcaaaa aatcctaacg gaatgatttc ccttgacttt
     2941 gtctttgatg ctagtgcatt agctggaaaa gagatagtcg tatttgaaaa tgtctatcgt
     3001 gacggacgct tagttgcgtc tcatacagat attcacgata aaggacaaac ggttcgtatt
     3061 gtgaacccta tgattcatac aaaagcaaca ggtcaaaacg gcgaaaaaca attacaagct
     3121 ttaccaaatc aaaaagtgaa agaattggta cagttgaacg acttaattgt tggtcaaacg
     3181 tacaaagtgc tcgtacaagg ttatattaca cctgatggtg tgaaattaga cggtacaagt
     3241 gctgaaaaaa ttttcaaagc agataagaca actttagagt tcttatttga atttttggta
     3301 gatggacgta agctagcagg caaaggatta tcttttgcag aatggttaga agcccaaagt
     3361 gaaccaaaag atgaaaacga ttatgaagtt cgatttgaac aaatcgcaaa ccacaacgta
     3421 gacttaaaat cagaagatca aacggtttat tttgtagaag ttccaaaagt accggaacca
     3481 aaaattcctg aaaaatcaca accttttaaa gaggtgaaac aagcttcagt agtaccaatg
     3541 ttgccaaaaa caggatcaga agatactgta ttatggatga tcattggtgt gctatttgtt
     3601 ttagcagcag gtcttacatt ttacgagtta cgcaaagtca aagactaagt aggacaaaca
     3661 agcggttaga tgcctaatcg cttgtttggt tttaaaaaag ttattgcata agtaaccttt
     3721 gaaaagataa gaaatgatct ttgttttatg aagaaaaaag aaaggaaaaa gagaatgaag
     3781 aagctatttt tcgcatgttt atttagttcc gtagttcttg ctggtggaat aaacgttttt
     3841 gcagatgaaa taggtattac accagttacg ccacctgtga tagaagaaac aacacctgtt
     3901 gttccaattc cagatatttc taaaatagat gagtctgttc cggatcaacc aactcctgtt
     3961 gatccggata tatcttcaga agtaactgaa ccaacaacac ctgttgttcc tgatataacg
     4021 gaaaattcag aaaatgaaac accagttgta tcgccttcta ctggagaaac aacgaatgat
     4081 aaagatacta caccaactac cagtgagtct tattcgggaa ctagttcggt agataatgat
     4141 tcaaaaatag acgattttag ttcttcatct actacaatag atacaccaaa agaaacagaa
     4201 aagaaaaatg atttaggagc tcccaaaaat ttaacacctg aaaatcatat aaagactgta
     4261 tcggaaaagg aaatgactgt ttcagtcagt tcagatggaa aaattgtaca aggaacgggt
     4321 accgaaaaat caattcctat tgtgacaagt aatttagaag aaattaatca tgtcccaaca
     4381 tctgaaactc ctttacaagt agcgactggt caagcgattg ttggggtaca agatggtgta
     4441 ccacttgtac aagataatca agggaatttg gtagaagatt tatccattcc tgtaaaaaaa
     4501 ctacctagcg gcaatattga aatcaaaaca atcgatggga aaacaaaagt attgccaaaa
     4561 acaggtgaag aacttcaaat tagtttaact atccttggtt ctcttttaac agcagtaacc
     4621 gggggcattt tttatggtaa aaaacaatgg cgtcatttgc gtcatatgtt taagaaaact
     4681 atttagtaag gagagagaat aaaaatgggt ttatcttatg ttgcaattag tcaggaagaa
     4741 gtattgggaa agtgttttaa tcaaaaacac gttggctcga caggtgaacg agatcgtcga
     4801 ggaaatatga agaaagtaga gtgggagatt tttcagatga actctgaaaa gatgggagaa
     4861 attgaagtgc gtgtagctgt cggtggtgcg cctaaagcca tgcgatacaa tgcagaagtg
     4921 ttactggcaa atcctagaat ttctcgtcga gcagatgtac gaaatattgg aaatgacggt
     4981 gaaacaaaca aaatagcacg taataggttt tatttgttag tcgatcgtgt agaagtaaaa
     5041 ggaggaaata actaatgaca ctagaatttg atcaaggaat tgtcccaggg ttggtaaaag
     5101 aaacactagg agaaatctat tttgtcaagg cacaacgtcc acgtatgctg tatgaagata
     5161 atgaaaacac aggagaaatt aaagagcgtc cagttgaatt ttcaagtgct acccaagaaa
     5221 caatgttcgt ggtgaatttt ccaccaaaaa caaatttgga tggattaagt tttggcgatg
     5281 tggtggaatt agaagaggtt gaatttcgat tttggatttc tattgatcgc gaatcaatga
     5341 gtaaccgagc agagaatgat ctgaaagtat ctgcgcttcg ttataaaaaa acgggaaaaa
     5401 acgtttttca aacgtcttcc tctactcgtg caacatatgt aaaagaaaaa caagactcaa
     5461 ataaagaaag tgcaaagaaa gagtaatggt ttccattgct ctttcttatt ttaaagctag
     5521 aagaagtagc tgatgtggag aaaattgaat tggctggcta tattattggg ataaattttt
     5581 gaatttttaa gaatttagat agtggttaga tacgattgat tctttgtgta ttttgtatta
     5641 tttaagtatt gtgtggtatt tacattaagt taatattgat gttgttagtg ttaatgaagg
     5701 ttttctttta tccaatacat tagtcactct atgatgcgag gagatgagaa taagtgtttc
     5761 aatataaagg tattcgtatt cgaaatcgac ataaatatgc ttcagaacga tttatttttt
     5821 atcttatgtt gccggttctg ttgctaatga gtggattttt ttctctcttt tatacaacga
     5881 tcacatggga atggtgctgt atttattgga taatcgcatt tggtttagct ggtctatttg
     5941 cgtatatgtg tttgtatttt tacttttata atgtttatca gcgattggtt cataaaggaa
     6001 aattagcaaa gatgatacat agtcgtcgat tgattcttga aagagtattg aaaaatggac
     6061 ggcggaaagt ggtgtattat ccaaaaattt tttatcacta taaaaatggc ttaattcatg
     6121 ttcgattgcc attagattta ggtccctttc aagatcggtt taatgagatg gctgggcaac
     6181 tagaagaagc ctttttttgt gatttagtag aagagcaacg agagaaaaac tatatttgtt
     6241 ataccttttt atacgatgtg gaaaaaaatc gtattgggat tcgagatata accgtaaaag
     6301 atggcataat caccttaatg aagaatgttc aatgggcatt taataaagca ccacatgcca
     6361 taattatagg aagtactggc ggtgggaaaa cgtattttat aatgtctttg atctatgcat
     6421 tattaccgta tggtacgttt gatatttgtg atccaaaacg tgcagatttg gctagtttag
     6481 aaaaaatacc aatttttaaa gatcaagttt tttatggtgg tggtatttta tcttgtttaa
     6541 taaatctagc taaagaaact gctaatcgat acgatttttt aagagaacag cccgagtatg
     6601 aattagggta cgattatact tattatcatc taactcctca ttttttggtg attgatgaat
     6661 gggcagcata ttatgcatcg ttagatacta aagaaaaaaa gaaggcaatg ggatgtttga
     6721 accaaattgc tttaatgggt cgtcaatgtg gcgtgttttt aattttaggt acccaacgtc
     6781 cagatacaaa gtatttggac ggcgcgatac gagataattt tggtttacgt gtaacactgg
     6841 gaagagtatc caaagctggg tataagatga tctttgaaca tacagtaaaa tcattattta
     6901 ataagccgat taaaggtcgt ggttatattg attcgggaac cgatcaagta cgagaatttt
     6961 atgcgccatt tatcgatata acaagattta acttttttgc ggaatttaaa caactatctg
     7021 atcaattaat ggctgaaaaa atcaaggaga cgaaagaggg aaaaaatgta tgaagaaatc
     7081 ccagctacat gtggaactcg attagaaata aaagatttaa tgattgactc ttttttggca
     7141 attgttttca gtgagaagag attaagcttc tttatcaata aaaaagtaga gaatcgttgg
     7201 agattagaac gtatgtatcc aacgagttaa ctgattggtt acagttgcct tatgacaatt
     7261 atcgaaggcg aatatgacgg gtttttactt caaataaaag tgctcgatcc tatagtgacc
     7321 aaattgtcga aaaagcagcg gaacagcctg ttttttatgg acgattcaaa ggactttcct
     7381 gttcttatgt taaaagtaaa gaccggtctc gactgtacct acaagtagag gatattaact
     7441 tgtcctttgc gttgtcagaa aattaaattt gtcctcatta gaatacggaa gatgctgtgc
     7501 gtgtgctcca caagcaaaca agcctcaggg cggaaggacg cggaagcgtc ctagcgttag
     7561 cgaaagccct gaggtgcagg gtgcgttgtg gggcgtgcgc gtagcgcacg cctaaaaccc
     7621 ccatattcta acaggggggt agaatttata ttcggtggca aagatgggtg attgaaacat
     7681 tgatataaca ctattttgct tcaaaaattt tgtgatattt ttcttcgctt tgtaacataa
     7741 aatatcacaa aagaaaggaa agatatattt ggatgggaca caattaaagc aaatgcggtt
     7801 aagtcggaat ttgacgcaga aattagtggc agatacatta ggatggccac aatctttttt
     7861 atcaatgata gaaaatgacc aacgaagttg ctcgaaagaa caagaagaag cactttgtga
     7921 attttatgcg aatttttcaa gttttacgag tttagaaatt aaatttgatt atgtccgtgt
     7981 acgagttccg acaaatgata cgaaaacagt tgtggaagaa attttgggaa tggattttaa
     8041 ctcttttttt tatgcagaaa cccgcttata tggttatatt gaaatgtatc aaaaaggcat
     8101 gatccgtgta ttaaattcta aaaaaggtga cgataaagga gtgttgatcg aattatctgg
     8161 tcaaggctgt cggaattatg aaagtgtact ggatgaacga aatgatagtt ggtatcaatt
     8221 ctttacacga tgtgtctctt tcaatggtgt gcctcgaaga attgatattg cggtagatga
     8281 ttttgaagaa ctattttctt tacatgaatt tgcacaaaaa ctaaatcgtg gcgaatttga
     8341 ttcaaaattt cgaacctggc ggtttcaaga agaaaaacag ttgttagata atctaagtag
     8401 tggcttatct gtttatttag gtagttcaca aagtttgatg tatttttgtt tttatcagaa
     8461 gaattatgaa attgcaaaaa agcagaaact tccgttggaa gaggtcgagg taaaaaatcg
     8521 ttatgaaatt cgcttacgtg atgcaaaagc ggtcagtttt attgaacaat acttaaataa
     8581 agtagatttt tcagatttaa cgatggggat tttagccaat cagttaataa tgtttgaggg
     8641 agagaaaaac gcaaatgtgt ggaaaccatg gggaaaattg attcagaacg ttggaaaagt
     8701 caatttgaca ataacaccag aaaagccaaa ttataatcgg aaattgatgt atttaaagac
     8761 acaacctgct cgtacaatga agattgtaaa aactgtggat gaaattttag gattgaatcg
     8821 atttggagaa attttagaag aaacagaatt acgggaacaa gatgaaaaaa ttgtagaaga
     8881 tgaggtgcgt gctgtatatg aatatataca agaaaatcca gacgcgattc attatttgct
     8941 ataaagcttg cgataagatg ggggaaacat gattaaaaca gaagaccaat gatggggaga
     9001 gtatatgatt gcttgttaac aattaataaa agaaacgatc ctatgtgttg taaatgatct
     9061 acagttattt aaagaatatt tagattatca acaatcaatc aaaatgttac atgtagaaat
     9121 aggggaagta ttaagctacc gtattagtga ttcgttaaaa tattcgttga caaagattta
     9181 tttatatgca acacaagtat gtggatatac gaaaaagcag taaaaacaat gatgaaatat
     9241 gacgatttgc caagcaagat atcctagatt tacaacaagt attggaagaa aaactgtatc
     9301 tatatatgaa aaatagaggg gaaaagaaat gattcgattt ttattttctg taggttggca
     9361 ttttttgaaa ttactgtttc ttttagtttg tattgtctgt agtattattt ggttattaac
     9421 actttaaaga aaggaatatg ttggaaaaat gggacagtat tttcttaccg gtgtttggta
     9481 tgtattgaaa ttagtgatta ttttaggtat tgcctatatt tgttttagaa ttttattatc
     9541 gggagaagga atgccaacgc caaaagattt tatcataagc gtgttgaatt ttgtg
//
LOCUS       DAXWLH010000102         9444 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXWLH010000102 DAXWLH010000000
VERSION     DAXWLH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.103"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..392
                     /locus_tag="ACGE36_001908"
     CDS             <1..392
                     /locus_tag="ACGE36_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFF8384290.1"
                     /translation="EIKRNKNSFGVYLGEPSSEQLNKGEARHALCRNIFYGRKGKLYQ
                     TYYDGMEEQLNALGLVTNAIIYWNSLYLEKVFEQIKTEGFDCSEEVLRKQFPLISDHI
                     NFVGKYSFRYEKELSNGILRPLNIENY"
     gene            557..772
                     /locus_tag="ACGE36_001909"
                     /pseudo
     CDS             557..772
                     /locus_tag="ACGE36_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            910..1881
                     /locus_tag="ACGE36_001910"
     CDS             910..1881
                     /locus_tag="ACGE36_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8384291.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2021..3334
                     /locus_tag="ACGE36_001911"
     CDS             2021..3334
                     /locus_tag="ACGE36_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFF8384292.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3377..4243
                     /locus_tag="ACGE36_001912"
     CDS             3377..4243
                     /locus_tag="ACGE36_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8384293.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4240..5088
                     /locus_tag="ACGE36_001913"
     CDS             4240..5088
                     /locus_tag="ACGE36_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8384294.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5102..6574
                     /locus_tag="ACGE36_001914"
     CDS             5102..6574
                     /locus_tag="ACGE36_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8384295.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6591..7463
                     /gene="scrK"
                     /locus_tag="ACGE36_001915"
     CDS             6591..7463
                     /gene="scrK"
                     /locus_tag="ACGE36_001915"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFF8384296.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7699..9081)
                     /locus_tag="ACGE36_001916"
                     /pseudo
     CDS             complement(7699..9081)
                     /locus_tag="ACGE36_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2869 a         1643 c         1970 g         2962 t
ORIGIN      
        1 atgaaattaa gcgcaataaa aattcttttg gtgtttattt aggagagcct agctcggaac
       61 agttaaataa aggtgaagct cgtcatgctc tatgtcgaaa tatattttat ggtagaaaag
      121 gcaaacttta tcaaacttat tacgacggta tggaggaaca gctcaacgca ttaggattag
      181 taactaatgc tatcatatat tggaattcac tatatttaga aaaagtcttt gaacaaatta
      241 aaacggaagg gttcgactgt tccgaagaag tgttacgcaa gcaatttcca ttaattagtg
      301 atcacattaa ttttgttggc aaatatagtt tccgctatga aaaagaatta tcgaatggaa
      361 tactacgtcc gttaaatata gagaattatt gagaatgttc gtcttttggt tcctaccgtg
      421 cattttcgca cggatgttcc agatactatt tttgaatgta taaaattgaa aacacaagaa
      481 tactaataaa tcaacgattc ttgtgttttt aatttttcac taccgtgcat tttcgtttct
      541 atgttccatg taccccaatc ttgaaaaatt aattaaagac ttagataaag ccattgacga
      601 cttagtcgtg gtgctaccgg agtatcaatg tttaaccagt atcccaggta ttggtcctgt
      661 ttaggctgct ggaatactcg ctgaaattgg tccaattgaa cgtttttctg atgaaataaa
      721 attgacaaag tatgccggcc tatattggcg acagagtcaa tccggtaaat ctgaatacga
      781 aaattttttt acgaaaagct tcacgtctaa tatctatttt aaaataaaaa tattcctgct
      841 aatttggaat cgtttccttc ttttggtata ataatacctg aaattctttt gagaggagtt
      901 gttcgtgaag tgaaaactac tatgaaggat gttgctgcgt tagcaggagt tggtgtgggt
      961 acggtatcta gagtaataaa tggagtaaag gtgaaagagg ttaccaaaga aaaagtaatg
     1021 gctgcaatta gtgagcttaa ttatgagcct gatgaatatg ccagaggtat gaaaacgaat
     1081 agtagcaata caatagctct tattttacct acgatttggc atcccttctt ttccgagttt
     1141 gcctactatg tagaggaagc tctcagccaa aaaaattata agcttttttt gtgtaatgcg
     1201 aacggcagtg cggaaaaaga aaaagaatat atccaaatgg tcaagcagag taaagtagac
     1261 ggaattatag gtattaccta ttcagacatt gataaatatg tatcatcaaa tttaccattt
     1321 gtcagtatag accgccattt ttcggaacag gtttcttatg taacggcaga taattattta
     1381 ggtgggcaaa ttgccgctca agaactactt actcgtaact gtaaaaactt gtgctatgtt
     1441 gggggtgttt caccttaccc taacgaaaca aacaatcgta aagctggttt ttacaatatt
     1501 tgtgaggaaa ataaaatagc tgtgaaggta ttggatatgc ctgaaccaat tagcaattta
     1561 gaggaacaat tgctcgcttt tttttcggat aatcctgaaa ttgatagcat ttttaccatc
     1621 aatgatttca tggctttgga tgtgataaaa gcccaaaaaa aactagggaa ggaagcacct
     1681 aaggattttc aaattattgg ttttgatgga gtcaagctat cgttgcaaaa agactattta
     1741 ctatcaacga ttgtacagcc gatagaggaa atggccaagg tttcagtaga aacactcatg
     1801 gcgatgattt taaatgaaga accgcctaag cgtgtggtag taccggtttt ctttcacgct
     1861 ggagagacga ctaaagagta aaaaaccggt ttataacgat ttttttgaga taggttgaca
     1921 gaaagtgatt tcaatgatat cttaaaacca attaattgga aacgttacca attaattgaa
     1981 aaataaaacg gaaacgtttc aaaaaatagg aggaacatcg atgaaaaaga gaatgttagc
     2041 aattgcagca attttagcat taacagctgg aggacttgca ggatgtggca gtaaggagaa
     2101 aagttcaggt agcagtgatc caggagaaat taagatttgg gttcagtttt ctgatgaaac
     2161 gcctgaagga aaagcttggg cagagattgt gaacaatttt aatgatgaat acgagggtga
     2221 atataaagct gttactgagt atattcctcg tagcggaagc ggcggtggat atgaggataa
     2281 agtaaatgct gcaattacaa ccaataccct tcctgatgtt attacgttag atggaccgaa
     2341 tactgcagct tatgctcatt caggagtcat tgctccatta gatgattatt tgaaagatgc
     2401 ggacatggat gatgtattag acagcattat tcagcaagga acctacgatg gcaaaatgta
     2461 tgcgtttggt ttctcagagt ccagtgtggg tgtctattac aataagtcgc tgttcaaaga
     2521 tgctggaatt acagaagatc aattgccgac gttagaagat ccttggactt ggacggaatt
     2581 tcaagcaatt tgccaaaagc tgactgataa atacaaaacg cctgcaattg atatgcaaat
     2641 ggcgtcggga gacgaaatgc tgacctacgc atatacacca tttatatggt cgaatggcgg
     2701 cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat tttaacggta aaaaatccgt
     2761 tgaagcgttg gaatttattc aagaattggt tgaaaaaggt tacaccacaa tcagtcctat
     2821 tgaaaaagga tttgaaactg gcaaatatgc catgatgctg agcggttctt ggacaatggc
     2881 agatttagag gaaaactatc aagagattga ttatggtatt ttgccttacc ccgtttcaga
     2941 tgcaactggg gagttagttt caccaaccgg aagctggcag ttagcaatga ctgagaaaac
     3001 agagaaaaag gatgcttctg cggcctttat taagtttgca accagtacag aatctagtcg
     3061 tattatgagt ttagggaaca gtgttctacc tatcagaaat tcaacagttg atttaattaa
     3121 agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa aaccaacttt ctggacatgc
     3181 ccgcccagtg gttgtagctt atccgcaagt atctagagca ttccaacaag cagttcaaga
     3241 tattagctac ttcgaggaaa acccagatgt acaaaaagta gcagatagtc gcgctgctga
     3301 aatgcaacag gcaattgata cttcactaaa atagaagttt ctaaaggggg ccaatcaagt
     3361 gttaaaaaaa ccgacaatga aagacaattt gataggttac ggctttctag cgccagcttt
     3421 gctgctgctg acgatttttc tgatcatacc cgtaatcatg gtaatttatt atgcctttac
     3481 tgattattat ttgttaacgc cagatgctag acaatttatc ggcttagaaa attttaagcg
     3541 actgttcgct gatccaattt ttgtaaaaag tatttggaac acggctaaat ttgttgtttg
     3601 gattattccg gtccaaatag gggcagcttt aggaatggca cttattatca acaaaaatcg
     3661 taaaggaaat atctttttta aagtagcctt ttttgcgcca gtcgtgatgt ccttagtcgt
     3721 tatttcgatt ttatggttgt acttgttaaa tcctaatgaa ggtctggtta atacgctatt
     3781 tgcaaagatt ggaattgctt cgcagccttt tttgactagt cccaaacaag caatgtatac
     3841 gcttgttttc gtatcggcat ggcaaggtgc aggatatcag atgctgctgt ttttaggggg
     3901 aatgcaaaat attccgcaag acgtttatga ggcagccgaa attgatggtt tttctaagtt
     3961 tcagcaattt cgctatatca ccatgccatt attaaagccc acggcgattt ttattttact
     4021 taccacattg atttcggcct ttaaactaat cattcagcca atggtaatga ctcaaggtgg
     4081 accaatgaat tctactatga caatggttta ttacatttac caaactggat ttacagaccg
     4141 tatggtaggc tactccagct ccattgcgtt aatctttacc actatgattg gtttaattac
     4201 aattgctcaa agaaaattgg taaaggagga tgatccagca tgaccaagaa atttggaccg
     4261 ctaatgatcg tagaatattg tttgctgttc cttttagcgg cattattcat tttcccgatg
     4321 ctgtggatgg tagtttcctc tatgaagcct gaagcagatg tctatcaaaa tttagcttcc
     4381 ttcaaagctt ttttacctag tttgaatcca gctaattggt ttaaaactta tcaagaggtt
     4441 atttcccggt tcagtgtggg aacttattta ttaaacagta ttttttatgg tttaaccttt
     4501 gcctgtgggt caatcattgt aaactctttg gcgggttttg cctttgcgaa ggttaatttt
     4561 tcaggcaaga aaattatctt tggagctttg ttagctctgc tgattgtccc gatggagacg
     4621 gttttaattc cgcaatttac catcgttaat tcactaggct tagtgaatac tcgcttggca
     4681 gtgattttac cagcgatggc cagcgcgttt aatatctatc tgtttagaaa tttctttatc
     4741 gcgattccag aagaaatcat tgaatcagcg aaattagatg gtgcaaatat tgttaaaatt
     4801 ttctttcaca tcatgctgcc aatgtctaaa ccagccatag ccacagtagg tgtcttatcc
     4861 tttatctcca gctggaatga ttatatttgg ccgttaatgg tcttaactga cagcagcaaa
     4921 ttttcaatgc aagtggcaat tactacgatc aatactacac agccagttta tatcaatcaa
     4981 gtgatggctg ttttaaccat tgcaacgatt ccactgatta ttgtttatat cgttgcacaa
     5041 aaatatattc tgcagggact tggcggttct ggaacgggta taaaatagga gtgaatagtg
     5101 aatgttgcaa aaacttttaa aggccaatga gtttattgag aaaaatgctc aaacagtcaa
     5161 tcaagaatac cggaataaat ttcatttgat ggctccgatt ggctggatga atgatccaaa
     5221 tggttttgtt tattatcaag aggagtatca cttgttttat caatattatc cttacgacag
     5281 catctgggga ccaatgcatt ggggccatgc taaaagcaag gatctaattc actgggagca
     5341 tttgccagta gcactggcac caggtgaaag ctatgacaag gaaggctgtt tttccggcag
     5401 tgccatagaa aaggatggca agctgtattt gatttataca ggacacaatg ttgtggaagg
     5461 gcaagttcga caagttcagt gtttggcagt ttcagaagat ggcattcact ttgaaaaata
     5521 tgccggaaat ccaatcatcg cagaagatca cttagcttct gttgcaacga ccgaagattt
     5581 ccgcgatccc aaagtgtttc agcgaaaaga agactatttt actgtagtcg ctactaaaac
     5641 tgcagatgct cgtggacaaa ttgtgatgtt ccattccaaa gatttactaa cttgggagtt
     5701 ttattccgta ttgctggagg gaactccaga acaaggcatt gtatgggaat gcccagactt
     5761 gtttgaacta gatggcaaag aagtcttgat tctttctcct attcaaatga ctccacaagg
     5821 caatgcttac caaaatacga actcaactgt tgctttcatc ggtactgtcg attggcaaac
     5881 aggcaaattt ctagtagaga attttcatga aatcgatggc ggtttagatt tttatgcacc
     5941 ccagacctgt gaaaacgaag caggccaaag aataatgatt gcttggatgc aaatgtggca
     6001 gcgtaaaata cctacgaatg atctgggcca tggttggtca ggttccatga ccttgcctag
     6061 agagctgagg gtggctgata atcaattgtt gcaacggcca gtttcatcaa tttatcaagt
     6121 aattgatccg caggaaagga ttgaatgggc gaagactgaa gagaatcgct tcttactgaa
     6181 ggacttagta tatgaccagc actatttacg cttggaaatc aatttaaaac aggcaacgaa
     6241 actagaactt cactatgcga agggacccga ccatacatta ctgcttaact atgatgttga
     6301 acgggagtgc tttactgctt ccagggaaca catgccgtat gatttatcag gagctgaaaa
     6361 ggaagtgttg aaaaagcgca gtgtgacgat tcctttggaa aataattgtc taaccatcga
     6421 aattttccgt gatacttcag ctattgagct ttttctaaat gatcgtctga ctatgagtac
     6481 aactttttat gagacagaaa aaggacagga ttttcttttg ctggcagaca gtgaagtaac
     6541 cttttcattg gaaagcggct taattaatct atagaaggga gagctcagtg atgatttatg
     6601 gttcaatcga agcaggcggc acaaaatttg tgtgtgccat tagcgatgaa gaaatgacga
     6661 tcaaggaaag agtgagcttt ccgacaacga cacctgagga aacaatacct ttagtcattg
     6721 acttcttcaa acaataccaa gcagatttag cagggattgg gattggctca tttgggccta
     6781 tcgatatcca tcgcgattcc gccacttatg ggtacattac ctcgaccccg aaagtggcat
     6841 ggcaaaattt tgatttcatc ggtaccatga aaaaagaatt tccgatcccg atttcttgga
     6901 caacagatgt caatgcggct gcctatggtg agtatgtctt cggcagtggc aaaggattga
     6961 gcagtgtcgt ttactatacg atcggtacgg gtgttggcgg cggtgcctta caagaagggc
     7021 gctttatcga agggtttagt catccagaaa tggggcatat gctcgtcgtc cctcatccaa
     7081 aagatgactt tgctggcagc tgtccatttc atggcaactg tttagaaggg atggccgctg
     7141 gacccgccat cgaaaaacgc ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt
     7201 attgggaaat cgaagcctcc tatattgctc aatgtgccta caatacaacc ttgatgtttt
     7261 ctccagatgt gatcattttt ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg
     7321 tccacgacgc atttgcaaaa ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat
     7381 atatcgtgac cccagcatta gaagacaacg ctggaacgct cggttgtttg gcattggctc
     7441 gagaagcggt cttacattca taaacgaaaa aaggctcttc gacatttagt gttggtagag
     7501 aaagatacaa ctgaaaattt agtcataacc agttaggcag agtgcttaac tggtttttgt
     7561 tttacttgga agatcaagcc ttgaatgaga taagttcttg ctcgaaagtt atggaaattt
     7621 cgatagccgt aggctacttg tattgtgcaa tagtttttta caaaaaaaag ggggtgcgga
     7681 tatttttttt ggaccttttt aacaaatata tctttgcttc ttccgatatt gaagagggct
     7741 tagccttcct aaagataact taattcgttg attattatac caatgaatat aatcatccaa
     7801 tttgtttata aactgattga ttgttgtatt cttccagtca cgttgataaa acatctcgtt
     7861 ttttaatcgt ccaaagaaac cttcgcacgc tgaattatct ggtgaataac tctttttaga
     7921 catagagtga agcaagttgg ctttgttcca atagtccaac taacaatagc accatcgtaa
     7981 caatcgacaa gtggtgataa gtagattttc cctgcgagaa ttctaaattc agtaatatct
     8041 gttaaccatt tttcatttgg cttctttgct ttaaaattcc gatttaataa attggggtgt
     8101 gcaggagaaa tttccccagc atacgaagaa tacttttttc gtctagaaaa tttaacaaat
     8161 agattttcct cagtcatcaa tctacgtaca actttttcag aaagcttttt acccatattt
     8221 tttaaagctt ggtgaattct acggtaacca tacgcacaga agttatgatt gaaaatcgtg
     8281 ataattaatt tgacgttctt tgtaatactt atctggtttc tcaagtgctt gtttttgata
     8341 aaaatagctg cttttaggaa actggagctg ttgaagaagc tctttcaagg tgaactgatt
     8401 tcgtaaggca tcaatcaata tcgttttttc ttgatttgat agacaaagaa gattgacgcc
     8461 tttttctttt tttagcagtt cagccgctcc ttcgagtaat gctttttcga gctgtaattg
     8521 gtgtacttgt ttttgtaagt cttctacttc ctttaaaagt agctcttttt gtttttcctg
     8581 attttttaaa gtcatacaca atggaaaatc ctttccaaga accgtgtttt tccattgata
     8641 tagagtaact ctactaattc ctccagattc ggctaaattt ttcctattag tttgcggttt
     8701 atagaatttt ttcaacacag cttctttttg atcttgtgtt aactttaccg cactttttcg
     8761 gagttttcta gctcctggtg caagttcttc acaccgctgc cgcaaggact ctctattagg
     8821 ataaccaagc atgcgaatgg ttcgtgcata acattgacca tattcaaaat aatgattcac
     8881 cgctattcgt ttttgttctt cagaatactt tgattttttt cggtgctcct gatggagctc
     8941 tccggattcc aaatactctt tataccattt ccgtagtgca ccaatcgacg gataccctaa
     9001 ttctcgaatg gttgcagcat aagagcaatc gtattttaaa aataactgaa ttgcccttac
     9061 acgctctttg tgggaaaaca cacggacatt cctcctaagt ttattttttc gtccaggatt
     9121 atgtccgcac ccccttgctt tctgatgact tattctggtt ctttgagtgt caatgcatag
     9181 atgtgatgta gctagttaga aaaaaatttt gaatactctt tttttctttc agactccttt
     9241 ttaagtaaga ttttgaagtg tgagttacgg ggtcagaaac ttaaaagttt ttgatccttt
     9301 attttcaagt ctgatttggc agcttttcgg cagctcattt atttatggtc agaaatagat
     9361 gctgaaattt taatctatta gcaaatagtc cagaacgtat gaagaggagt cggatatcat
     9421 ttgtcattaa caccataaat attt
//
LOCUS       DAXWLH010000103         9301 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXWLH010000103 DAXWLH010000000
VERSION     DAXWLH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.104"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(869..1210)
                     /locus_tag="ACGE36_001917"
     CDS             complement(869..1210)
                     /locus_tag="ACGE36_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8384297.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1210..1440)
                     /locus_tag="ACGE36_001918"
     CDS             complement(1210..1440)
                     /locus_tag="ACGE36_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8384298.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1727..2065
                     /locus_tag="ACGE36_001919"
     CDS             1727..2065
                     /locus_tag="ACGE36_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384299.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2270..2832)
                     /locus_tag="ACGE36_001920"
                     /pseudo
     CDS             complement(2270..2832)
                     /locus_tag="ACGE36_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3564..4145)
                     /locus_tag="ACGE36_001921"
     CDS             complement(3564..4145)
                     /locus_tag="ACGE36_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8384300.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4328..4954)
                     /locus_tag="ACGE36_001922"
     CDS             complement(4328..4954)
                     /locus_tag="ACGE36_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8384301.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4976..6307)
                     /locus_tag="ACGE36_001923"
     CDS             complement(4976..6307)
                     /locus_tag="ACGE36_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFF8384302.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6462..6863)
                     /locus_tag="ACGE36_001924"
     CDS             complement(6462..6863)
                     /locus_tag="ACGE36_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8384303.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6949..7155)
                     /locus_tag="ACGE36_001925"
     CDS             complement(6949..7155)
                     /locus_tag="ACGE36_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384304.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7250..8752)
                     /locus_tag="ACGE36_001926"
     CDS             complement(7250..8752)
                     /locus_tag="ACGE36_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFF8384305.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8770..9225)
                     /gene="argR"
                     /locus_tag="ACGE36_001927"
     CDS             complement(8770..9225)
                     /gene="argR"
                     /locus_tag="ACGE36_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8384306.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
BASE COUNT         2631 a         1936 c         1485 g         3249 t
ORIGIN      
        1 ctttagaatt tttactcatt catagacact tccttaaata acttttatta tagaaggtat
       61 tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaacttac attatactgg
      121 aatgaactgt attgatagtg tacctaaaag ctctactgac ataagatcgt gtaatcggta
      181 aagtaatttt attaattata cgggatgtaa tcaattagag aataaactaa tcataaaagg
      241 tacttttaca ttcataattg tcgctttgag attctacttt ggttcactat ctgacccaat
      301 tgcacatttt cgatcattac aagtattggt gtcgttagaa caactaaata aaaactagcg
      361 gtagattttt cagaatctat gctaatttct gtatggatgt tccagtgatg gcaaatttct
      421 cttttcattg tttttcttgt cgcatgatat gcctttttgt gttattcagt taaactatag
      481 aaattctttc ctagacagat cttgatccca aattttacgt ttttgagcat cgatatagtt
      541 taaaatgaca ttactcatat ctaaagagtt tagatttttt aggactaaaa ctgtcagttg
      601 ttctttttct tctttctgtt aaagtattgt cgcgactttt cgtatacgtt aagatatgtc
      661 gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact tgaggaaatc acctcaatca
      721 ttcgtttaaa atgctatata ttctttagtt taagatacgg aaattaatgc catcgaccac
      781 actagtagaa tgaacgacat ttacttggcc tttttttaat ttcgttatgt ttagcatata
      841 aaaatattat agagtttact tacaatgctt aaaacatata ctgtataatc tgatcaattt
      901 tagccatgtc ttgcaatggt aagttttcta tttttttgag ttttcgttcc ttaaaatcaa
      961 gagattttaa ttgtgtaata attacttgtc cttttatatc tagctcttca ggtaatgagt
     1021 aacgtgtcgg taaattcttt atagtggaag taattggaca tatgaccgaa aacattgttt
     1081 tacggttaaa ttcataacga gagacgacta gtcctggtcg tcttttttga atttcttttc
     1141 ctacggaggg atcaaaatca atccatacaa tatcaccttt tttaggaata taaattcttt
     1201 cttctgacac tataaaattt ctctcccttc aggagttaaa tcttcccatt cgtctttctc
     1261 ataatactcg gcctcttccc caccactaaa tggatcttca atttttggaa ctaacgtaat
     1321 tgttccatca tcagaataca caacgatata ttcttggttt tctgaaggtt tctttccgtt
     1381 atcagaaggt aaagttacca ctacagaact tccttgtaat ctagattttg taacaagcat
     1441 tatcatcact cccttaataa aagtatacta tataacacta agtaacgcaa aattcccctt
     1501 tgtaaactat ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat
     1561 aaattgtagc cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct
     1621 atggatccaa aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga
     1681 taaaacgctt tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa
     1741 acaaaaagct ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact
     1801 agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt
     1861 ggttcaacga atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat
     1921 ttttgtagaa gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc
     1981 tcttatctat aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag
     2041 attccaagaa ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc
     2101 gcagcacgag taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata
     2161 gatgcagtat atgcagctta tgagtatttc ttctgttctc tttgtttttg ggatctgtgc
     2221 tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct
     2281 ccattactaa ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta
     2341 cccaaggttc gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga
     2401 ctgtgcggtt aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc
     2461 tacgataaaa accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt
     2521 tttcttcatt ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt
     2581 ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc
     2641 ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt
     2701 cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct gcttcggttg
     2761 tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata
     2821 acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt
     2881 tcgcaccaac aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa
     2941 ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa
     3001 acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac
     3061 gacctctggg taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     3121 aaatgtagaa agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc
     3181 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
     3241 cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt
     3301 tttcacaacc tctccactaa acgatgttca atcagctaac cttcggtatt agctcaaaaa
     3361 acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct
     3421 gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa
     3481 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
     3541 acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg
     3601 atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata
     3661 cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt
     3721 ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat
     3781 tctagtagca acagacggta ttcttcatcc actgtactca actcaataat caaattacct
     3841 acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt
     3901 tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc
     3961 tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag
     4021 taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt
     4081 tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt
     4141 ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat
     4201 tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat
     4261 aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat
     4321 tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa
     4381 agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg
     4441 tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca
     4501 agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc aattggagcg
     4561 ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat
     4621 ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat
     4681 attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa
     4741 attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt
     4801 tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga
     4861 tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag
     4921 tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt
     4981 ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga tctctcaata
     5041 acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat
     5101 cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga
     5161 atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga
     5221 ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata
     5281 aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat
     5341 tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata taagtgaact
     5401 gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt
     5461 catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg
     5521 atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt
     5581 cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta
     5641 cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg
     5701 cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag
     5761 atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca
     5821 ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac
     5881 ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc
     5941 gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa
     6001 gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat
     6061 ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga
     6121 ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg
     6181 ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta
     6241 acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt
     6301 ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga
     6361 taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg
     6421 tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct
     6481 acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca
     6541 attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc
     6601 tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct
     6661 gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct
     6721 gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc
     6781 atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt
     6841 gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc
     6901 tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac
     6961 cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag
     7021 cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat
     7081 tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac
     7141 catattcgtt accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc
     7201 ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg
     7261 ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact
     7321 tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta
     7381 aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt
     7441 cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta
     7501 atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta
     7561 cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta
     7621 attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt
     7681 tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac
     7741 ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga
     7801 tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga
     7861 ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc
     7921 gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa
     7981 tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt
     8041 tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac
     8101 ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat
     8161 ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc
     8221 cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta
     8281 cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt
     8341 ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa tagggaagat
     8401 ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa
     8461 acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac
     8521 tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa
     8581 tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc
     8641 cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta
     8701 atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt
     8761 cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc
     8821 tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa cttcagggta
     8881 agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat
     8941 aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg
     9001 ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc
     9061 attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac
     9121 atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc
     9181 tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca
     9241 atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta
     9301 t
//
LOCUS       DAXWLH010000104         9205 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXWLH010000104 DAXWLH010000000
VERSION     DAXWLH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.105"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            246..521
                     /locus_tag="ACGE36_001928"
     CDS             246..521
                     /locus_tag="ACGE36_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384307.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            534..2612
                     /locus_tag="ACGE36_001929"
     CDS             534..2612
                     /locus_tag="ACGE36_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8384308.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2775..4658
                     /locus_tag="ACGE36_001930"
     CDS             2775..4658
                     /locus_tag="ACGE36_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8384309.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4670..5617
                     /locus_tag="ACGE36_001931"
     CDS             4670..5617
                     /locus_tag="ACGE36_001931"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8384310.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5637..6164
                     /locus_tag="ACGE36_001932"
     CDS             5637..6164
                     /locus_tag="ACGE36_001932"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8384311.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6227..6880)
                     /locus_tag="ACGE36_001933"
     CDS             complement(6227..6880)
                     /locus_tag="ACGE36_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFF8384312.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7012..7854
                     /locus_tag="ACGE36_001934"
     CDS             7012..7854
                     /locus_tag="ACGE36_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8384313.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8012..8908
                     /locus_tag="ACGE36_001935"
     CDS             8012..8908
                     /locus_tag="ACGE36_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8384314.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8911..>9205
                     /locus_tag="ACGE36_001936"
     CDS             8911..>9205
                     /locus_tag="ACGE36_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 family protein"
                     /protein_id="HFF8384315.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENE"
BASE COUNT         3193 a         1555 c         1911 g         2546 t
ORIGIN      
        1 gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc
       61 ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact atttggaata
      121 gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga
      181 aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct
      241 ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc
      301 tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt
      361 atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt
      421 gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa
      481 aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat
      541 ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca
      601 ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga
      661 cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac
      721 cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga
      781 caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt
      841 tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag
      901 gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg
      961 cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc
     1021 agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc
     1081 tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa
     1141 aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg
     1201 aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca
     1261 ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg
     1321 atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg
     1381 cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag
     1441 acggtttata tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt
     1501 ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt
     1561 tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca
     1621 gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt
     1681 ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg
     1741 tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga
     1801 ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa
     1861 aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt
     1921 acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt
     1981 tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg
     2041 gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt
     2101 tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc
     2161 cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata
     2221 tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata
     2281 acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg
     2341 aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc
     2401 tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg
     2461 aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt
     2521 tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag
     2581 caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa
     2641 aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa
     2701 gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat
     2761 aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa
     2821 aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg
     2881 ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg
     2941 acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag
     3001 cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct
     3061 tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag
     3121 accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt
     3181 ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata
     3241 aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga
     3301 ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga
     3361 tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg
     3421 atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat
     3481 acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag
     3541 aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg
     3601 ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa
     3661 aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac
     3721 gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac
     3781 tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa
     3841 atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg
     3901 gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa
     3961 tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag
     4021 cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta
     4081 tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga
     4141 aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca
     4201 tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg
     4261 tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa
     4321 acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga
     4381 cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga
     4441 agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac
     4501 ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc
     4561 gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg
     4621 cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc
     4681 gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg
     4741 cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc
     4801 tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt
     4861 aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg
     4921 ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg
     4981 ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt
     5041 ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat
     5101 ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa
     5161 acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg
     5221 gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta
     5281 tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg
     5341 cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg
     5401 tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat
     5461 cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa
     5521 tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga
     5581 cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt
     5641 tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt
     5701 ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga
     5761 tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg
     5821 ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag
     5881 acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg
     5941 tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat
     6001 tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta
     6061 gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta
     6121 atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga
     6181 ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata
     6241 aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca
     6301 tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg
     6361 ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga
     6421 cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt
     6481 tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa
     6541 ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg
     6601 caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa
     6661 aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga
     6721 tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca
     6781 ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat
     6841 ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag
     6901 gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat
     6961 gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat
     7021 gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc
     7081 attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa
     7141 ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc
     7201 caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa
     7261 gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc
     7321 agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta
     7381 tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc
     7441 cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta
     7501 gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg
     7561 aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga
     7621 ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca
     7681 aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa
     7741 gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt
     7801 atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa
     7861 agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta
     7921 ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca
     7981 ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct
     8041 cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc
     8101 gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga
     8161 aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa
     8221 gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc
     8281 ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt
     8341 aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt
     8401 tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc
     8461 ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg
     8521 atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa
     8581 ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac
     8641 ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg
     8701 attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga
     8761 aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa
     8821 tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca
     8881 atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac
     8941 aaaacaaaga aaacaaatta ctggaaattc gcattcctta tccttcttgg ggtagtactt
     9001 ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa
     9061 ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa
     9121 aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa
     9181 tataaatttt atttggaaaa tgaag
//
LOCUS       DAXWLH010000105         9001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXWLH010000105 DAXWLH010000000
VERSION     DAXWLH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.106"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(69..1208)
                     /locus_tag="ACGE36_001937"
     CDS             complement(69..1208)
                     /locus_tag="ACGE36_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFF8384316.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE36_001938"
     CDS             complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE36_001938"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFF8384317.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE36_001939"
     CDS             complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE36_001939"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFF8384318.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE36_001940"
     CDS             complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE36_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFF8384319.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE36_001941"
     CDS             complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE36_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8384320.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6222..7313)
                     /locus_tag="ACGE36_001942"
     CDS             complement(6222..7313)
                     /locus_tag="ACGE36_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFF8384321.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE36_001943"
     CDS             complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE36_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFF8384322.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8856..8927
                     /locus_tag="ACGE36_001944"
     tRNA            8856..8927
                     /locus_tag="ACGE36_001944"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8889..8891,aa:Arg,seq:ccg)
BASE COUNT         2336 a         1905 c         1658 g         3102 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtttatta
       61 aaaaaatctt acgctaagta ggataaaata attccttgat atttttcgat catatccaaa
      121 taattatcga tttcaacata ctcatctact tgatgaggca aagtaacgac accaggccca
      181 aagacgacaa agtcgaagga atggttagct ttcgtaaatt cggctgcatc cgttgttgct
      241 gccgctccaa ctaaaggtaa tggctggctg aattgttgct gaatgcaatg aatcaaagga
      301 gaatctggat cagctttgac aggtattttg ttgtaatcga tcatcaattc caaatgatag
      361 tctgtctctt gattcaattc attgacgatt gattgcagca atgcaatgat tttatcattt
      421 gggtattctg gaatcgatcg aatgttccct tggagctgcg catggcttgg gatgctgttg
      481 acttgattcc cgccactgat caatgtaaca ttatggattg tttttcctag gacaggattc
      541 tcgattgttt cagcgaggtg attcatttca gcatttgctt tcgtaatgaa ttcatttagg
      601 tgattgattg cattataacc ttggtctggc atagagctat gcgcttcttt tccgtgcgag
      661 gtgactgtat agttgattga tcccatgtgc gtatacatca aactgtaatt ggttggctct
      721 ccgatgatca aggcatctaa gtcatctaca tagcctgctt tcgtgagctg ttccccacct
      781 agttcaccca cttcttctcc gacagtggct aataatttga ctgttccgtt aaatggtttt
      841 cctgactctt tcagttcgat catggcaatc accattgctg ccaagccact cttcatatct
      901 gtagcaccac gtccgtacaa acggttccca tggatttctg ctgcaaaggg tgcataggtc
      961 caagacgatt catcaccagc cgccacgaca tccatatgcc cagataggcc taacactttt
     1021 cctgattggc ccttttggta acgagcaatc aaattatctc taccatctct gtaagaaaca
     1081 atctctcctg ttatgtcatg ctttgccaaa agcttattca aataggctgc tacttcacct
     1141 tcgtttccat tcactgattt aattcggata atctcttgta aaatcgcgat tttttcttct
     1201 tttttcatct aatcctctcc cttgccattt ctttttattg tatttgagtg tgctcgaatt
     1261 ttcaaatagg agcaaccaaa gtaaaaatga agaaagagag ccactttatg aaaaatatgc
     1321 tgctacttcc ctcctatttt atttatacga gaggacttgc ccatagattc ctattctact
     1381 gttctatcct taaaagctct tatatgatgc tagttattgt tagctttcct ttttcaagga
     1441 tgatcttttc ttgtaccatt ctttgtaaac atcatataca gaagctagtt gcaaaaaaac
     1501 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
     1561 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
     1621 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
     1681 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
     1741 ggccagcgtt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
     1801 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
     1861 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
     1921 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
     1981 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
     2041 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
     2101 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
     2161 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
     2221 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
     2281 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
     2341 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
     2401 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
     2461 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     2521 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     2581 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     2641 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     2701 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     2761 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     2821 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     2881 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     2941 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     3001 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaagaa tgaatctgct
     3061 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     3121 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     3181 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     3241 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     3301 acctgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     3361 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     3421 tttgcaaaga tagctttcgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     3481 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     3541 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     3601 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     3661 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     3721 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     3781 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     3841 ttgttcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     3901 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccgccac ctgttgagat
     3961 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcggctgagt ctccgccacc
     4021 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     4081 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     4141 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     4201 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     4261 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     4321 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     4381 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     4441 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     4501 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     4561 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     4621 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     4681 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     4741 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     4801 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     4861 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     4921 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     4981 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     5041 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     5101 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     5161 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     5221 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     5281 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     5341 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     5401 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     5461 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcgggaa
     5521 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     5581 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     5641 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     5701 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     5761 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     5821 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     5881 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     5941 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     6001 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     6061 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     6121 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     6181 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     6241 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     6301 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     6361 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     6421 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     6481 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     6541 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     6601 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcgcg gtggttaccg
     6661 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     6721 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     6781 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     6841 ggaagtagtc ggtctaaggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     6901 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     6961 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     7021 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     7081 tttttcaaaa ggtctgtttc tgttcgtaag actcgttctg tgatccccat actttccgat
     7141 aaacttcgac gcccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     7201 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     7261 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     7321 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     7381 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     7441 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     7501 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     7561 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     7621 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     7681 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     7741 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     7801 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     7861 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     7921 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     7981 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     8041 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     8101 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     8161 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     8221 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     8281 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     8341 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     8401 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     8461 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     8521 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     8581 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     8641 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     8701 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     8761 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     8821 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     8881 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     8941 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     9001 a
//
LOCUS       DAXWLH010000106         8936 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXWLH010000106 DAXWLH010000000
VERSION     DAXWLH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.107"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(398..895)
                     /locus_tag="ACGE36_001945"
     CDS             complement(398..895)
                     /locus_tag="ACGE36_001945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFF8384323.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(935..1744)
                     /locus_tag="ACGE36_001946"
     CDS             complement(935..1744)
                     /locus_tag="ACGE36_001946"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8384324.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2078..2935
                     /locus_tag="ACGE36_001947"
     CDS             2078..2935
                     /locus_tag="ACGE36_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8384325.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2928..3830
                     /locus_tag="ACGE36_001948"
     CDS             2928..3830
                     /locus_tag="ACGE36_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8384326.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3863..4213
                     /locus_tag="ACGE36_001949"
     CDS             3863..4213
                     /locus_tag="ACGE36_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8384327.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4262..5185
                     /locus_tag="ACGE36_001950"
     CDS             4262..5185
                     /locus_tag="ACGE36_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8384328.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5263..6156
                     /locus_tag="ACGE36_001951"
     CDS             5263..6156
                     /locus_tag="ACGE36_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8384329.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6194..6757
                     /gene="amaP"
                     /locus_tag="ACGE36_001952"
     CDS             6194..6757
                     /gene="amaP"
                     /locus_tag="ACGE36_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8384330.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6771..6959
                     /locus_tag="ACGE36_001953"
     CDS             6771..6959
                     /locus_tag="ACGE36_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8384331.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6972..7874
                     /locus_tag="ACGE36_001954"
     CDS             6972..7874
                     /locus_tag="ACGE36_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8384332.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7906..8148
                     /locus_tag="ACGE36_001955"
     CDS             7906..8148
                     /locus_tag="ACGE36_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8384333.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8404..8775)
                     /locus_tag="ACGE36_001956"
     CDS             complement(8404..8775)
                     /locus_tag="ACGE36_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384334.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3120 a         1544 c         1727 g         2545 t
ORIGIN      
        1 cctacaaggt acataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
       61 aagtaaaaac agcttcttat gtttttactt ggctgttctt attactgcag atgcaagctt
      121 ttgaataccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagcttaa
      181 caaacggaaa aatagttcct catattttgc cgtttgttaa gctaataccg agggtcagct
      241 gattgaacac catttaagaa agaggtcgtg agcctgccgt ttcactctgt gtcataaaaa
      301 agccaatcta ctttgataga aaaagcacgt aggactaaaa cagttctgcg tgcttttttt
      361 attgtgtgtt aaatatcgta aatgaagata gatattttta tgattcgacg atagagacgt
      421 tgaaatcttt caacagctct aaagcggatt ctccgtgttt tctactggag actaacattg
      481 gatcaacaaa aaagtctgct tcaggaaaag cactctgcag gcctatcaga ttagagagta
      541 cacacaagtt tgtttcaatc ccgactactt cgatctcttt ttgttcttct ttgctttcaa
      601 ataaagtttc ctggatttca gctaaagttt caggtggaat cgtaaagtaa tactttttaa
      661 tcactcgttc attttcttgt ggagaaagaa gagggatcaa ttgcaaatct tctcgttctt
      721 cttctttgtc cttcagttca actggtatat cccgtgtgaa gagaatatat tcattatttt
      781 ggcgtgcttt agctaatctt tgagcaatac gttctgccaa ttgatctgac tctggaatat
      841 aaaattcgct gtttggatca agaatatgat tttgcatatc aataataact aacattttct
      901 ttcctcctac attcatatcg tcattttatt ctttttaata gaagtttttt aaaatataag
      961 caacaccagc ttcttcattc gtcagtgtta cttggtttgc aatttctttt acttgtcgct
     1021 gtgcattccc catcgcaata ctcaacccgc tgtaattcaa catagcaatg tcgttttctt
     1081 gatcgccaaa agccatgatg tcactcgatt cgattccaag gtaatccgcc agaaaaacaa
     1141 gtgaatgagc tttagtgata cctgtaggcg tgatttctaa gaaatgcgga tcagagaata
     1201 ctgattggat accatctgct tgcagatctc tttgtagctt ctgtagtttt tcttcactct
     1261 tatctgttaa tgctaattta agatagctaa gagaaggatc ttccaacata tttaaaattt
     1321 gatcaaaaga atggttctgt aaattgaaat gttcataata agggtcatct ttgactagat
     1381 ttgattcatc gaatgtcagc tgatcgtctt gggtttctcg gctagcaatg atggatattt
     1441 tcgcttcttg agcatagaag aaggcttttc tcagtgtatc gctatctatt tttgaacgtc
     1501 tgagaacttt cgccattttt ccatccttca tttcagcaat cagtgaacca ttggaagaaa
     1561 tcaaatagcc accgattcct aattcggaaa aaatatcttt agcagaaatg ctgttccttc
     1621 cggtagcgac ggttacgatt ccgccatttt catcaaattt ccgaagtgct tggatatttt
     1681 cttctggtat ctttccattt cgatcaagca aggtaccgtc taaatcaatc gctgctagtt
     1741 tcattttatc ttcctacttt ctttttacat atctattttc ctgttacctt aagattaaat
     1801 gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat gaattttttc aaaaaacatt
     1861 cttaagaaaa aggtggctat atatagtagt ttttggtcaa tacctttgaa taaaaagcca
     1921 tgaaaaacga atagttagcg cttaccgatg cgttttgtga taaaaaaatt ttaaaatatt
     1981 caatgaaaac aaagcatata ttttgcttta aaaccaaatg aggaatattc tagatgtaga
     2041 acaggttaga aaaagattag aaaaaggagt gattttgatg gagaagagat acggcgtagt
     2101 tcctgtggta actgcttcag acgaaaatta tgctccttac ttgagcgtga tgatcgcaac
     2161 tgcattagaa aattgcaata aaacaagacg cataaaattt tatgtgattg acgatggtct
     2221 atcagaatat agtaaagaag gattggaaga aacggtaaat aaatattcaa gtaatgcaag
     2281 tatccaattc ctgacagtgg aaaaagatat ctacgaagac ttcctagtca gtgaccatat
     2341 cacaactacc gcttacttaa gaatttcttt accaaactta ttagctaaag aggattataa
     2401 gaaagtcctt tacttagatt cagatgtgtt agtactagac gatatcgtaa aactatatga
     2461 tgagccgtta aatggaaaaa caatcggcgc tattatcgat ccaggccaag tgaaagcttt
     2521 ggaaagatta ggaatcgatt ccgatgactt gtacttcaat tcaggagtga tggtgattga
     2581 cattgatcaa tggaataaaa aagaaattac agaaaaaact atccattacc tcagtgaaaa
     2641 tggcgatcga atcatctatc acgaccaaga tgctttgaat gctgtacttt atgaagattg
     2701 ggaacaactt catccaaaat ggaacatgca gacttcgctg atttttgaac gtcatccagc
     2761 accaaatgaa aaatatgaac gtcagtataa agaaggaaat gaaaaacctt cgatcgttca
     2821 ctttacagga catgataaac cttggaatac gttgaaagat catccgtata ccaatttata
     2881 cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc gagatcaatg aataaaaaag
     2941 aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc acatttagct gcattatttg
     3001 tctcagtatt agataattgc aacccgtcta aatttgttcg gttctatgtg attgacgatg
     3061 atatcgattt tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca cgaatgaact
     3121 cagatgtgga attcttgaaa atcaataaag aattctttac aaatgtggta atcagcgatc
     3181 gtattccaga aacagcttat tatcgtattg cgattccgga attgttccgc ggcacagaag
     3241 ttgaacgtat cttatatatg gattgcgaca tgatcgcttt acaagatatc tctaaattat
     3301 ggcgtcttga cttcggtgat tcaatcgtag cagctgttga agacgctggg ttccatcaac
     3361 gactggaaaa aatgaaaatt tcagctaaat caatgcgtta tttcaactca ggtttgatgc
     3421 tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca aaaagtatta gatttcatcg
     3481 aacataatcc agaaaaatta cgattccatg accaagatgc attaaatgct atcttacatg
     3541 accgttggtt accacttcat ccgcgttgga atgcacaagg ctatatcatg gcaaaagcaa
     3601 aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga aactcgcaac aatccttaca
     3661 ttatccattt ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg ccaacgaaga
     3721 aatattacga aaaatatgca gggatgacgg ctttccgttg tgtagctaaa ttccctaaat
     3781 atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa tagaagacca
     3841 caactgaaga gggggagtag gaatggaaat tacaatgtct aaaaatgcag tagagacgtt
     3901 aattgaaaag gttggggaaa atacaaaaat cgcactcgcg cttatcaatg attccgatcc
     3961 atttctaagg gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca ttccttttgt
     4021 ttcggaattt ggagaatatg cgactaaaat cgagcatcca ttattggaca tctatacatc
     4081 taaattagag caaaattatt tcggtaaacg attgaacatg gacttcaaca aacagcttga
     4141 cagcttttca ttagaaaatg aaatcgcagt actcgattat aatatcaaat taaagaactg
     4201 cttcttcagc taatagtttc gagaaagtcc aaaaaggagg tatacattga gatactactt
     4261 tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa aaatataatt ggctcgtact
     4321 tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc ttcctgatga
     4381 tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc tttatgagac
     4441 gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag ataatcgtat
     4501 cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga ttacttgtat
     4561 cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc atttaaactc
     4621 gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg tgaaagaact
     4681 aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa caacggaaaa
     4741 tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg atcatacctt
     4801 aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca tcaaacgttt
     4861 agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag aaaaaatgat
     4921 cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga tgatggacaa
     4981 taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt ccgttccggc
     5041 gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa gttctgctct
     5101 aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag gttaccattt
     5161 ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa acaaagaaat
     5221 ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga accaaaatta
     5281 atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca ggatacacca
     5341 gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta tgaaggcaat
     5401 aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat cggtcgtgca
     5461 gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt cccaggagaa
     5521 gaaaaagatg cagaggaagt agcaaaatac gtgaaagatg caggacgtaa gattgtctta
     5581 ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa agccgtgagt
     5641 gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc ttgtcagtct
     5701 attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat catcgctatg
     5761 tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat cgtaacaaca
     5821 acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc aacaaaagct
     5881 tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg aatccgtgta
     5941 aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg acaaccttct
     6001 gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca gccaggagaa
     6061 ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac agcccaaatt
     6121 tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt cacccctatg
     6181 aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa cattactttt
     6241 gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc cttttcagct
     6301 agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg tattttggct
     6361 tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt tttatccaaa
     6421 aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg ataaaaaagc
     6481 aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt caccaaaagt
     6541 aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt tgaaacgaac
     6601 ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg aagtacaaca
     6661 catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct ttgaagaacc
     6721 agctaaacga agcaatgacc gcaaacgagt agaatagggg gggaccaaaa atgaaagaac
     6781 tattttcgac atacaagctc cctatcatct ttggggtact tggattgata ttagccgttt
     6841 tatggatcac cgtgggcttt ttcaagacct tattgatctt gatcatggca gcgatcggcg
     6901 tcgctatcgg tttttatctg aaacaaacga aaatcttaga tcactatttt aaatcttagg
     6961 aggaaacatc catgaataac aataacaaaa ataataacaa taaaatgaac aaccaaaaac
     7021 cagtgtctgc acatccagac ccaacgacac caaaccaatc aaatggtgtg aacgatgaaa
     7081 agaacaaaac agcaccagta tctgcacatc cagatccagt agtccctaac gttcaacaag
     7141 gagtagaaga gaagaaacat gatgcagaaa agcatgcacc tcatcatcca ggaactccag
     7201 aatctaaagt tccagaacaa catgatgcaa tcgatccttc taaacctgtg gaagagcaaa
     7261 aacatccttc tgaccatgcg aaagcacaag aaaaacttag ttctgaccac acaaacggac
     7321 aaggaaaact tggttctgat catacaaata accaaggaaa gcatgattct gaacaggaag
     7381 attcaatgcg cggtgagctg aattatgaag ataaagttgt gcaaaaaatc atcggaatcg
     7441 caattgaaca agtagacggt ttattatctg caaacggtgg tttcttctct aatgtggcag
     7501 gaaaacttgt gaatacagat aatgtaacgg ctgggatcga aacagaagtc ggtaaaaaac
     7561 aagtagcagt tgatatggat gtcattgtgg aatatggcaa agatatcgaa aaaatctttg
     7621 aagaaatgca agaagtaatc ggcaaagaag taaaaaatat gacacattta gaagtgatcg
     7681 aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa gatcaagaaa
     7741 cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg tgaatttgct tcgaatcaaa
     7801 cagataaagt aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa aatacagaac
     7861 cacgggttca gtaataagga agtaaaacat taggaggaac gaaacatggg atttatttgg
     7921 tcacttatcg tcggaggaat tatcggagca attgcaggag caatcacgaa taaaggttct
     7981 tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg gatcagcact tggtcaagca
     8041 ttattaggtt cttggggacc aagtattgca ggaatggcaa tcgtgccatc attgattggt
     8101 gccatcattt tagtactaat cgtatcattc tttgctcgaa aactgtaaaa agacttttaa
     8161 aaagaggtcg tgaaaaagct gttcttcatc aagtaataag aacagccaaa caaaaatagc
     8221 ttctcatatt tttcatttgg ttgggcatat tatcgaagat gcaagctttt gaacactgtt
     8281 tatacggaat aagaggctgt gacaagactt ttgtcacagc ctcttattta tgcgggcaaa
     8341 ttcccaatta gatttttaaa agtttttcct taaatgtgtc tttattgata cagagaagct
     8401 tctttaataa aacaatgcct ccctaaattt tcttgagata ggaaaggtga acatacaaaa
     8461 taatacgacc actttgaaaa cccagatgat ccacaaatgg atcgtatgat actgatgaag
     8521 taaaaagcaa tggatcagaa actcaaagag attgaaaaag acaaggaaga aataatatct
     8581 cttttttact cttgtctgtt ttagccaata atagctcgta aagtaatcta gacaaacaaa
     8641 tagaagtaaa caaagtttcg taagagcagc aatcgtatca tgttgctctg ctagacgatt
     8701 gacgagcagg aaaaatgttg gtaatggaat atagatcacg agtaatgaaa gaaaaaagta
     8761 aacaataaat ggcatactat tcctcctaat tgtgtgagtt cgccctcatt ttaaaataac
     8821 gatatttgtt tgtgaagctt tttcgtgtcg taacaaatgt tatctaatgt atttttaaat
     8881 aaataatcat tattatcaaa ataatgttaa aaataaaaaa gaattgtcca tcactg
//
LOCUS       DAXWLH010000107         8909 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXWLH010000107 DAXWLH010000000
VERSION     DAXWLH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.108"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..777
                     /locus_tag="ACGE36_001957"
     CDS             <1..777
                     /locus_tag="ACGE36_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384335.1"
                     /translation="PDKNKFETETKDLRTVGYTSSEKVLTNKDMTNNQVNYSAVVRTV
                     KERANNNPVKEFNENINYKFTDNTKTKTGYGVSVDLEPNYRNEIGKQIDMKVYSYPMN
                     ELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGINTQNLSFQFPHMNIERKTGNIYA
                     DSQKNDESIKYELIDGGRKLYVPIWCDLGNYSIAIKSSNFGGNYISLDMNQNIDVYAY
                     MYATIDSETKKEDELLLEPVYPESTLPDGWNKNEIDWLNK"
     gene            837..1199
                     /locus_tag="ACGE36_001958"
     CDS             837..1199
                     /locus_tag="ACGE36_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384336.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSMVFTDMVHKMLNGEPINFNFDYMLEKAQSNQVVAFLVSTYYFIEKSIFSLKDF
                     FLGIFTKLTTFYNKILKN"
     gene            1213..1365
                     /locus_tag="ACGE36_001959"
     CDS             1213..1365
                     /locus_tag="ACGE36_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384337.1"
                     /translation="MLDQNTDRSWWMIGAVIVGAVLIGTAKVAFPEVFDTVITFFKNM
                     IPTNLG"
     gene            1433..1855
                     /locus_tag="ACGE36_001960"
     CDS             1433..1855
                     /locus_tag="ACGE36_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384338.1"
                     /translation="MIYVALFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETTD
                     YIFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            1867..3282
                     /locus_tag="ACGE36_001961"
     CDS             1867..3282
                     /locus_tag="ACGE36_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFF8384339.1"
                     /translation="MRVVEEKKISPKIEENPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSANEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            3284..4114
                     /locus_tag="ACGE36_001962"
     CDS             3284..4114
                     /locus_tag="ACGE36_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384340.1"
                     /translation="MKVQAMTKRKGLMDVSELNAYQVAYGKPLDYRDYFNYVGLPCLF
                     MGGFSFALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQIL
                     TDDSKTVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVF
                     TQYFEQLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAV
                     FIGAITFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN
                     "
     gene            4114..4911
                     /locus_tag="ACGE36_001963"
     CDS             4114..4911
                     /locus_tag="ACGE36_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384341.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKTDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIKPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            4929..5291
                     /locus_tag="ACGE36_001964"
     CDS             4929..5291
                     /locus_tag="ACGE36_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384342.1"
                     /translation="MQEQSVMEIIKWFIGLIMIMTMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDGRFTIESDQLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVALSFNGNSVSQIR"
     gene            5305..5451
                     /locus_tag="ACGE36_001965"
     CDS             5305..5451
                     /locus_tag="ACGE36_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384343.1"
                     /translation="MQENNVSRIIYLIGGLIILSLAIAGLVEHWPEIKLLIRNFYDNK
                     MIKM"
     gene            5463..5684
                     /locus_tag="ACGE36_001966"
     CDS             5463..5684
                     /locus_tag="ACGE36_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384344.1"
                     /translation="MEAIQENRKDTQETIEKNKKTRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            5703..5978
                     /locus_tag="ACGE36_001967"
     CDS             5703..5978
                     /locus_tag="ACGE36_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFF8384345.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            6087..7913
                     /locus_tag="ACGE36_001968"
     CDS             6087..7913
                     /locus_tag="ACGE36_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384346.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSWGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELAVSPEEPEEVEEIETVSENEEEVEEIFDFEDEEEVENVQKGDLS
                     TSSQNELVAVNDSQVPPYMPSQTPNYSPQYQAPNVLQPVRKHETVQLVTLNEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDVAWKKPYDLDVKKDKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            7926..8642
                     /locus_tag="ACGE36_001969"
     CDS             7926..8642
                     /locus_tag="ACGE36_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384347.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGKPTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     KKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEVKSVDRRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            8655..>8909
                     /locus_tag="ACGE36_001970"
     CDS             8655..>8909
                     /locus_tag="ACGE36_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384348.1"
                     /translation="MKLVQNNEVKERKNGFVKNSNKILGKLKKVGGFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKAL"
BASE COUNT         3375 a         1106 c         1745 g         2683 t
ORIGIN      
        1 ccagataaaa ataagtttga aactgaaacc aaagatttaa gaacggtcgg ttatacttct
       61 tctgaaaaag ttttaacgaa caaagatatg acaaataacc aagtcaacta ttcagcagta
      121 gttagaacag tgaaagaaag agcgaataat aatccggtta aagagttcaa tgaaaatatc
      181 aattataaat ttactgataa tactaaaaca aaaacaggat atggtgtatc agttgattta
      241 gaaccaaact acagaaatga aattgggaag caaatagata tgaaagtcta tagttatccg
      301 atgaatgaat tgattgacag caaaattgac gaaatctatc aaagtggggg aaaagaaaca
      361 gaggttaaga tggatcgctt aaagaatgat gggaaagttg agtcgggtat taacactcaa
      421 aatctttcat tccaatttcc acatatgaat atcgaaagaa aaactggaaa tatatatgct
      481 gacagtcaaa aaaatgatga atctattaag tatgaattga tcgatggcgg tagaaaatta
      541 tatgtgccta tttggtgcga tttggggaat tattctattg ctatcaaaag tagtaacttt
      601 ggtggtaact atatctcgtt ggatatgaac cagaacatag atgtttatgc ttatatgtat
      661 gcaacgatag atagtgaaac taaaaaagaa gatgaattat tgcttgaacc tgtttatcca
      721 gaatccacat tgccagatgg ttggaacaaa aatgagattg attggttgaa taaataagtt
      781 tgaaaagttt ttatctatct gatagaatta aacaatgata tagggatggt gatagaatga
      841 gggttcaaag ctatggtaac agtgtttttg atgttaaaag agaatttcaa gattcgagaa
      901 gaaaaaccaa aaagctgata gcttacttca ttctatcagc tatcgttttc tatattgtca
      961 agtttgtttt ggaattaaac tcaatggtat tcacggatat ggttcacaag atgttaaatg
     1021 gagaacctat taattttaac tttgattata tgcttgaaaa agcgcaaagt aatcaagttg
     1081 tggcctttct tgtgagtact tattatttta ttgaaaaatc tattttttct ttaaaagact
     1141 ttttcttggg gatatttacc aaactaacaa cgttttacaa taaaatttta aaaaattaag
     1201 gaggaagttt gaatgttaga tcagaataca gatagaagtt ggtggatgat tggtgccgta
     1261 atcgtaggtg ccgttttgat tggaacagct aaagtagctt ttcctgaagt atttgataca
     1321 gtaattacat ttttcaaaaa catgatccct acaaacttag gttaattcca ctaaaaaatt
     1381 cacgctgtta aatgcgtaga aaaataataa agaagaaaaa gggctacata aagtgattta
     1441 tgtagccctt tttttggatg gaagtgatgt aatgctaaga gatatgccca aaatgattct
     1501 tctgatgttt gttgggttct ttttaatttt agtgattgcg tactattcat taaatggcta
     1561 tacacaagat tcttcagtga aaggtatgca agaaacatta agaactacag caatagcaaa
     1621 cagggatgat agtattcgtg tagaaaaagg gcgttttgct ttaattaaat ctgattttga
     1681 aaaagatttt aaagaaaaat ttgggaaaag taaaaatcta aataatgaaa ccactgatta
     1741 tatatttgat tatcttacag atggaaagga tggtattaaa gcaattaaag tgaaggttga
     1801 aagtggatca aaagaatatc aagcaacttg tattttaaat gttgcaaatg attagaggtg
     1861 tgaaatttgc gtgttgtaga ggaaaagaaa atttctccaa aaatcgagga aaatcctaaa
     1921 agtaaaatta atatcgcaag tattattaaa aaaaatggaa ttaaagaaaa ctctgaagcg
     1981 atagaaggaa atcttgattt tttaactaag cttttagaag aagatagtgg aaattatgaa
     2041 ccaccaattg aagatgcaaa attgaatatg attcgtgatg atttaaccag aaactattcc
     2101 caagaagttt tacaagcttt aactgatgat gaaattaggc gaaaacttgt acaagttgtt
     2161 ttgaaagaac atggtacttt ctttaaaaaa gataaagagg ttgcagaata tgtagtacgt
     2221 gaatgtattg gaacagggat tattgagcga attatgacca acgatgaaat tacagatgtt
     2281 ggttggaatg gctctcaact tagcattgaa actaactcta acaaatggtt gtttgacggt
     2341 gataaattaa atatcacgca agagtatatt gaacgtgtta tttctaaata tgctgttgta
     2401 aatgatcggg cttttaatag tggtaatcct atcatggatg gaatgtttaa aaatgtccgt
     2461 atatcagcta cacacataga taattcacca gatggctcta cgttttctat gcgtgtggca
     2521 aggcctagat tagcattgaa taaagaaaac tttgaagatt ttgctcctga ttatattttg
     2581 gagctatttg agaaaatgat gaaaacaaag gcaaatatct gtatatcagg ggagacaggt
     2641 acaggtaaaa cggaacttca aaaattactg ctaagtttta ttgataatca tgatcgtatg
     2701 attatgatag aggacgttca agaaactcat gccaaagaat tgtttcctga taaagatatt
     2761 ttctcttgga tcactactcc caaaaaagca atttcagatt tagtaaaagc ttctcttaga
     2821 aataatccac gatggatcat tgtttcagaa acacgtggtt ctgaagctta tgaaatgctt
     2881 caagcggttt tgtcaggtca ctatgttgta acgacacttc atgcggttaa tgcacgtgcg
     2941 atacctagac gttttgtaaa tatgtgtgca agtggtttta aaataaacga aaatgcggtt
     3001 gaggaagaca taaatcgtta tttcgatttt ggtgttcata tcaaaaaaac agtggttgat
     3061 ggtaaagtga tccgttattt gaatgaaatt gttgaatttt ctgctaatga accgactaca
     3121 gtttttaagc aaaaatttat caatggaaag tttgtcattg aaaatggtag tctttccgaa
     3181 gattttaaag aaaaaatggc tgaaaaattt attacgtttg aatttcctga agatcaaact
     3241 aatacagaag aatcacttcc tgaagaagaa gaggtgattt aagttgaaag tacaggctat
     3301 gacaaagcgt aaaggtctca tggatgtttc ggaactaaac gcctatcaag tagcgtatgg
     3361 taaaccgtta gattatagag attattttaa ctatgttgga ttaccttgtt tgtttatggg
     3421 tggcttttct tttgctttat gttactactg gtggattgct ttgatttgtg gagtaattgg
     3481 aggcgtatat ggctttaaag ttataatgcc taaaagtatt aaacgctctt atgaattaaa
     3541 gagcttacag gaaagaaata aatttattaa caacatgact caaattttga ccgatgatag
     3601 taaaacagtt actagagcgt tatctatagc aaaacagcga acaaaaggtg aattaagaga
     3661 agatattcaa atcttagaat cacgtttaca aggtgctgat aaatttcaga ttgctgaagc
     3721 ttttcgtgaa atcaacaata aatattatag tgatgttgtt tttacacaat actttgagca
     3781 actggaaaca gctatttacg aaggtagaaa taattcagat actatgaagc aaataaaaag
     3841 ttatcacaat gatgtgaaac gtaaaacaga aggttttatg aaaatcaaag atggctatct
     3901 tcaagatatg aagcaaatga tgtttattgt agctgttttt attggagcta taacttttgc
     3961 ttttgggttt tctactttct atgaaggctt tgctcgatca cctatcggat gggtatttgg
     4021 gtctatttac tttttgatta tggttagctt catgaagtca tttttcaaac attattttga
     4081 tgatgaaata atgagtttgg gggtaagtaa ttaatgaaag ttaaggcttt gaaaaagaaa
     4141 agaaaatcaa gtattagccc cttgaaaatt acgaaaacag aacataaaag cttttggatt
     4201 aaattgctag gtgatgttta ttatcaaaaa ttacgtgaaa tgaacaattc caatattgcg
     4261 attattaagt ggcaaaaaca acggttaaaa aagtcattga tttttatcct ggtcggattc
     4321 ttaatgtatc tatttttagg gaaagaatgg tattacttag ccggaggaat tgctacttca
     4381 gtaggttttt atgtttggca agcaaaatca atcaatggta tctataatca atttcggttt
     4441 gaacgacact tgcaatttag taaatttaca cgtctattga ttccatatct gaaacaaaaa
     4501 acagatggcg gaaatctata cggtgttttt gcaaaaatcg taaaaagact agattatgat
     4561 accgataaaa agctattaat gaagcttatg caagaaatga cagatcagcc taacgatatt
     4621 aaacctttta cggaatatgc agaacagact tccggaacag atatgtccgt attatttatg
     4681 tctacagttt atgatattcg acaaggatcg gcagatttag atgtgattga agagttggat
     4741 cgattagctt ctgaagaatt gatggccgga atcgattcaa tcattgacta taagtcgagg
     4801 cgttttgtct tctttcctac aaaaattgca atgacttcat ttattttagt tgtcggtttt
     4861 gcagtaggcg ttcttttcat gaacctaaaa gatattaact ttgggcaata aaggaaagga
     4921 gaaagtcaat gcaagaacaa tccgttatgg aaatcattaa atggtttata ggtttaatta
     4981 tgattatgac aatggtttct ataggactct tttgtgttca attacaagat attaatactt
     5041 ttaagcaaca agtaaattac caaattgaac gaaaaggagg tctgactaca gaagctgttg
     5101 aagcaatcga caagtattcg gctactaatt atgatgggcg ttttacaatt gaaagcgatc
     5161 agttaaatca gaaggtttct tatggagaag aaattgatta tacgatcaaa gcaactattc
     5221 caattcaaat tttaccgctt cctgatgtag ctttatcgtt taacggaaat agtgtttctc
     5281 aaataagatg agaggaagga taaaatgcaa gagaataatg tttccagaat aatttatttg
     5341 attggcggat tgataatttt atccttagct attgccggtc ttgtagagca ttggccagaa
     5401 ataaagttat taataagaaa cttttatgac aataaaatga ttaaaatgta ggaggtatag
     5461 atttggaagc aattcaagaa aatcgtaagg atactcaaga aacaattgaa aagaacaaaa
     5521 aaacaagaaa aaagaaagta gataaattta cggaaatgac cgataaaatc attgaaaaga
     5581 aaggacttaa acctaaagac tttgaccgac aagcaatcaa agaagcacga caaaaactgt
     5641 atgatgattt tgttaaagaa aacatatcac agcttttaga ctaacattta gggggaaata
     5701 aaatgaagaa tttagttatc agcaagtttg caatgttacc aagaatggat tttttcgata
     5761 atgtaagtaa gtttatgtca aatatgactt caaaactaca aggaatttcg ctatctgtaa
     5821 ttatcttttg tattgtggtt actggtttga tgtttctttt cggagaaggg ccaagtcgta
     5881 ctgctaaaaa atggttaacg tatatcgtga ttggggcaat aattatcttt ggagcttcaa
     5941 ctatcggaca aacgatcaag tccgttggtg gtttctaagg ggaagcttct tagaactaaa
     6001 aaattcgcaa gtattgcatg aaagagcatt gagtgattaa tgctcttttt tattttgaaa
     6061 aactataaac taaggagatt taaactatga accaacgatt cgaatataat tttgaagatg
     6121 tagaacctaa gttgttgaaa aaaggaaaag ggttaaaaaa gaaaaataaa tatactctca
     6181 aacaagcaga agcactagta ggatggattc aacacaattg gtcagacaag acaacaatta
     6241 ccttgatctg ttcagataca gaagaaggaa caaagtatgc ttttgatgga attgaaatta
     6301 ctccattttc gtggggcgga caagaaaaac tattgtttcc tgatattgaa ggtcaaattt
     6361 tacaagcgcc tgacagcatg atttccgatc ctaataaact gattttggat attaacgatc
     6421 aatacaaaca cgaaccagac gtagcagaac caacaaaaga atcaaagtca aaattaaatc
     6481 tgaagctccc ttcagtagta ttaccatttg ctaagaagaa aaataaactt atgaatgaat
     6541 tgattgaacc ggaagaaacg gatatttatg aagaagaatt agaagaacaa gaagagattg
     6601 aagaattagc tgtttctcct gaagaaccag aagaagtaga agaaattgaa actgtttctg
     6661 aaaatgaaga agaagtagaa gaaatttttg actttgaaga tgaagaagaa gtggaaaatg
     6721 tacagaaagg cgatttgtct acaagtagtc aaaatgaatt agtagctgta aatgattcac
     6781 aagttccgcc atatatgcct tctcaaactc ctaattactc tccacagtat caagcgccta
     6841 atgtcttaca accagtaaga aaacacgaaa cagtgcagtt agttacgctt aacgagtact
     6901 gtgatttggg agaagaaatt gaaacttcaa tttctcaaat ggtcgaaaag ttgaaaccag
     6961 aatctttatt caaattcata gggattcctt ctacaggtct tacaaatact cgtattgatg
     7021 aatatagaag taaccatgct atgttgcgat tagctgaagt aaaatttgaa aatttaagag
     7081 agcattattc acgacaaatt atttcattta aagcagaagc actagatcgt ttaagaactg
     7141 cggtagatgt cgcttggaag aagccttacg atttagatgt gaagaaagac aaagccgaag
     7201 agttatcaaa aatggaagca gaagcagaag ctaaattaaa tgagttcgtg aaacgtcagg
     7261 aaaacaatgt aaatgataaa atcaaaaagt ttgagatcga acaagaaata gatttaaaaa
     7321 actttattgc aagacaagag gcagacaaag cggtattcat tgctacagag aaagatcgga
     7381 ctcaaaattt aattgattct aaagcggaat caatcaatgc agatttactt gaaacaaaag
     7441 ataaatttat tgatgaagaa atgtacaaaa tgaaaattga aatgaacaaa aatttatttg
     7501 atggcaagca aaaaattaaa tcagaattgg ctactgaact cgctaaggca aatgataatg
     7561 tttgggagaa tgcgctgaaa tttattcaag aaattcaaga tgaaattgaa gctaaaacac
     7621 caatttgggc tgctgaaatc aaggagttta atagtttaga agaacaagaa catgaacgta
     7681 tgaagcaaac taaagaactt caattaaaag aggaaagtat tgaaatcaag cgtttggaag
     7741 ctgaaatgaa tttgaagcgt atggaagaat tagatcatga aaataaaatg cttaaaatta
     7801 aacttgaaac tgccctttca aaaaatgagt tactagctat ggaacaagat acaaagcaag
     7861 tggtgaatgg atcaaacaga gttggcgcac ttaatttatt tagtaaacgt taaaggagaa
     7921 cggatatgag tgtttttaat aataaagtta tgttagggaa ctgttatgtg aaccacattg
     7981 gtttttacga tggaggaaca tacagagttt tattcttacg tttatctgtt tttagttact
     8041 caaaagatgg aaaggggcat tatgaaaata ttaatgcaca aatttcttgt cctttagata
     8101 gaaatggaaa acctacgact acaagtagtc accctaaagc cttgatgaat aaatttatct
     8161 ttgaaaagtt ggatcgcaat ttggttgagg ggatgaagat aagtattcat ggaaatattc
     8221 gtggaacaga acaagtgctt cttaacagtg gttggaaaaa ctttggtcaa ttatcgcctt
     8281 tagaaaaaga agaatatcaa aaaattccta aaaatgaatt agtaacaaga aatcaaacat
     8341 atatctatat agaagaggta agtatgccag aaaaagaaga atttagaaag tctcaatcta
     8401 aggtaaaaaa agaagatgtg gaagttgcag aaccgaatca agcagatgaa attttatccg
     8461 atgatactga tccagtgaat gatgaaggag aggttattga agtttttgaa gctgaaatta
     8521 tcgaagagga agaagaagtt aaaagtgttg atagaagaat gtatcgagag aaaccaaaag
     8581 gttatgtgaa ttttgaagat atgtattatg acgaatattc taatgaacca ccgatcaatt
     8641 agtgggggtc aaatatgaag ctagtacaaa acaatgaagt gaaagaaaga aaaaacggct
     8701 ttgttaaaaa ctcgaacaaa attttgggaa agctgaaaaa agtaggtggt ttcaaaaaaa
     8761 ataaatatca tactggaaaa attgagggag aagatttacc gaaaagttta caagataaat
     8821 tgaatgttcc gcttgtattt ataggtgttt ttttaggttc aatagttttt ctagcaataa
     8881 catacttatt gaacttttta aaagctttg
//
LOCUS       DAXWLH010000108         8869 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXWLH010000108 DAXWLH010000000
VERSION     DAXWLH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.109"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACGE36_001971"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACGE36_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFF8384349.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACGE36_001972"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACGE36_001972"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFF8384350.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACGE36_001973"
     CDS             complement(2169..2906)
                     /locus_tag="ACGE36_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8384351.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACGE36_001974"
     CDS             complement(2997..3524)
                     /locus_tag="ACGE36_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384352.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACGE36_001975"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACGE36_001975"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFF8384353.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACGE36_001976"
     CDS             complement(4401..4886)
                     /locus_tag="ACGE36_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFF8384354.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACGE36_001977"
     CDS             complement(5039..5236)
                     /locus_tag="ACGE36_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384355.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACGE36_001978"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACGE36_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFF8384356.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACGE36_001979"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACGE36_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFF8384357.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2500 a         1971 c         1486 g         2912 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgata
//
LOCUS       DAXWLH010000109         8812 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXWLH010000109 DAXWLH010000000
VERSION     DAXWLH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.110"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            1..74
                     /locus_tag="ACGE36_001980"
     tRNA            1..74
                     /locus_tag="ACGE36_001980"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Met,seq:cat)
     gene            93..165
                     /locus_tag="ACGE36_001981"
     tRNA            93..165
                     /locus_tag="ACGE36_001981"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Asp,seq:gtc)
     gene            169..241
                     /locus_tag="ACGE36_001982"
     tRNA            169..241
                     /locus_tag="ACGE36_001982"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Phe,seq:gaa)
     gene            246..316
                     /locus_tag="ACGE36_001983"
     tRNA            246..316
                     /locus_tag="ACGE36_001983"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Gly,seq:tcc)
     gene            329..402
                     /locus_tag="ACGE36_001984"
     tRNA            329..402
                     /locus_tag="ACGE36_001984"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:363..365,aa:Ile,seq:gat)
     gene            414..485
                     /locus_tag="ACGE36_001985"
     tRNA            414..485
                     /locus_tag="ACGE36_001985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:447..449,aa:Glu,seq:ttc)
     regulatory      674..753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            751..1944
                     /gene="metK"
                     /locus_tag="ACGE36_001986"
     CDS             751..1944
                     /gene="metK"
                     /locus_tag="ACGE36_001986"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8384358.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2074..3546
                     /locus_tag="ACGE36_001987"
     CDS             2074..3546
                     /locus_tag="ACGE36_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8384359.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3629..4417
                     /locus_tag="ACGE36_001988"
     CDS             3629..4417
                     /locus_tag="ACGE36_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8384360.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4484..5152)
                     /locus_tag="ACGE36_001989"
     CDS             complement(4484..5152)
                     /locus_tag="ACGE36_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFF8384361.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5267..6979
                     /locus_tag="ACGE36_001990"
     CDS             5267..6979
                     /locus_tag="ACGE36_001990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384362.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6982..8754
                     /locus_tag="ACGE36_001991"
     CDS             6982..8754
                     /locus_tag="ACGE36_001991"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384363.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2644 a         1509 c         1963 g         2696 t
ORIGIN      
        1 cgcgggatgg agcagtctgg tagctcgtcg ggctcataac ccgaaggtcg taggttcaaa
       61 tcctgctccc gcaattaaaa actaaaaaca ttggttccgt ggtgtagggg ttaacatgcc
      121 tgcctgtcac gcaggagatc gcgggttcga ttcccgtcgg aaccgtttgg ctcggtagct
      181 cagttggtag agcaatggat tgaagctcca tgtgtcggca gttcgattct gtcccgcgcc
      241 attatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
      301 attctcgtca cccgctttta gtttttatgg gcctatagct cagctggtta gagcgcacgc
      361 ctgataagcg tgaggtcgat ggttcgagtc catttaggcc catttatttt attggcccgt
      421 tggtcaagcg gttaagacac cgccctttca cggcggtaac acgggttcga atcccgtacg
      481 ggtcatggaa gatacttttt gagtatcttt ttctttttat ctgaaagaat gattttaaaa
      541 agcttagcag agttatcttt atggatgatt gtgctaagct tttttggaca gttctaatgt
      601 aaaatatttt tatatttttt aagcaaaaaa atgaaaaaat acgagcagaa aactgtttta
      661 tctgctatac ttggttcgtt gcaagttccc gaaaggatcc gcaggacgga gatgccttgt
      721 aaccgaattt aactaggggg aatcattata atggtagaaa gacacttatt tacatcagaa
      781 tctgtttctg aaggacatcc agataaaatt gctgaccaaa tcagtgatgc aatcttggat
      841 gcaattttaa aacaagatcc aacagcacga gtagcgtgtg aaacgtctgt aacaactggt
      901 cttgttttag tatttggaga aatatctacg acggcatatg tagatattca aaaagtcgta
      961 cgggaaacaa taaaagaaat tggttataca cgtgcaaaat tcggatttga tggagataca
     1021 gcagctgtat tggttgcgat tgatgaacaa tctcctgata ttgctcaagg agttgatgaa
     1081 gcacttgaga tccgggatga agataaaaaa gacgtattag atgagattgg tgctggtgac
     1141 caaggtttaa tgtttgggtt tgctgtagat gaaacaccag aattgatgcc tttaccaatc
     1201 gctttgagtc atcgtctggt acgacgcttg gcagatttgc gtaaatcaaa tgaattaacg
     1261 tatttacgtc cagatgcaaa atctcaagta acggttgaat atgatgatca aggacaaccg
     1321 gaacgcgtag atacaatcgt tatttcaaca cagcatgatg atgcagtaga taatgaaaca
     1381 attcgtcatg atgtcatcga aaaagtagtg aaggaagtta ttccagctga attattagat
     1441 gatcaaacga aatattatat caatccgact ggccgatttg tcattggtgg tcctcaaggg
     1501 gatgccggat taacaggaag aaaaatcatt gttgatacgt atggcggtta tgctcgtcat
     1561 ggtggcggtg ctttttctgg taaagatgcg acaaaagttg accgttctgc cagctatgct
     1621 gcacgttata ttgctaaaaa cattgttgca gcagggcttg ctcgcaaagc agaagtacag
     1681 ctagcttatg cgattggtgt tgctcaacct gtttcgatct caatcaatac tttcggtact
     1741 ggaacggttc cggaagaaga actgattgct gcagtgagag aaaacttcga tcttcggcca
     1801 gcaggaatca tcgagatgct tgatttgcgt cgtccgattt acaaacaaac agctgcttac
     1861 ggtcattttg gtcgtacaga tgtagatttg ccttgggaac atacggacaa agtagatgcg
     1921 ttgaaagcaa gtttagcaaa ataagttata ttcagctgga acgatcgcca agtggtggtt
     1981 gttccagctt tttttatggc agttaacctc ttgaaaaaga tagtaattat ttatctattt
     2041 ttagatcctg tcataatgaa agggaggaat aagatgaaaa aagaaacaaa tgttactttg
     2101 gtcacagtga gtattttcat tgcaacgttc atgacggcta ttgaaggtac aatcgtaaca
     2161 accgctatgc caacgattgt cggttcatta catggaatgg aaatcatgaa ctgggtattt
     2221 tcgatttatt tgctgacaaa cgctatgctt actccgattt acggaaaact tgcggataaa
     2281 gtgggacgga aaccaatctt tatttttggg acattattgt ttattatcgg ttcgttattt
     2341 tgcggtttat ctcaaagcat gattgggttg attgctgctc gtgcgattca agggatgggt
     2401 gctggggcaa tgatgccagt agctttgacg atcatcggcg atctttattc tattgaaaag
     2461 cgagcaaaag ttttaggttt gaatagttca gcctggggga ttgccagtgt ttttggaccg
     2521 cttgcgggag gattcatcgt tgaaacgatc agctggcact ggatcttttt tatcaatgtg
     2581 ccgatcggaa ttctattaat tattttgatt tggctatatt taaatgaacc aaaaatcata
     2641 catgagtcta aaccaatgga tatttttggt agttttagtt tgatggccat gcttgtctca
     2701 ttgctgttag ggttccaatt attaggagac gaaggaatga gtccgcttgt ttctgctttg
     2761 ctgctaggta gtatcgtaat gttaggtatt tttgtttttg ccgaaaaaag agctaaagat
     2821 cctgtcatca tgatgcattt attcaaaaat ccactatttg ttgtggtcaa cttggctgca
     2881 gcattggcta gtggtttttt gatgggaatc gatgtttata tccctatgtg gatgcaaggt
     2941 gtattagggc tgaatgcagc aattggtggg ctggtattag caccattatc actagtctgg
     3001 atggtcggtt ctttccttgc aagcaagtgg atgactcaaa tgaaaactca ttgggtcttg
     3061 agaattggac tactaatcgc agctattggg ggcgcttggt tgtatcttat gcctttcaga
     3121 agtaactatt tatggttttt tgctatatca acggtcatcg gtataggctt aggtgtgacc
     3181 atgactacct cgactgtcag tgcacaaaat agtgtatctc gtgaccagct tggtgtagca
     3241 acttctttta atacgttagt tcgaacaatt ggtcaaacag tcatggtttc tatttttggt
     3301 ttattgataa atggggtaac taaacgagaa cttgcagcag caaatttaac aaatgatgca
     3361 gatatcatga ataaactggt gaatccacaa acggctaaat atattgatgc ggatattttg
     3421 ttaccattaa gaaaaattct ttatgatggg ttacattccg tttatctagt tggcttgtta
     3481 ctcgtgatca ttgctttggt cttaacgctt ttcgttcatg aaaaaagtgg taaaataaaa
     3541 cagtagtttc ggttaagata tgtacgtagc cgaatcattc aaatttaaag ttattcaaaa
     3601 ataacttctt actaaggaag gcgaaaatat ggcatttatt caagcaaata tttattcgaa
     3661 tgtgttagaa atggaagtca atctaaatgt gatactaccg caaacaacaa aaaaggtgat
     3721 tggcaccagt tcagagcaag agacaacgga tatacctgtt ttatatttac ttcatggaat
     3781 gggtggcaat catagtgtat gggaaagacg tacgtcgatt gaacgttatg tagcaaacta
     3841 cggattagct gtcatcatgc catctactga tcttggctgg tacacagata cagtttatga
     3901 catgaagtat tggacattca ttgcagaaga gctacctgag atttgtcatg aattattccc
     3961 gcaattaaca agaaaaagag aaaaaacata tgccgctgga ttatcgatgg gcggatatgg
     4021 cgcgttaaaa ctaggccttg caaaatcaga aaatttcgca gcagtaatct ctctttcagg
     4081 cgctgtttct gtaggaaatc gaatggatga tcttttgatg gttcgtaaag catctttttg
     4141 ggaagggatc tttggaccat tggatcaaat cgaaggttca aaaaatgatc cgcaatactt
     4201 attgaaagaa ctagtaaaaa gcggaaaacc agttccaaga ttttatatgg cttgtgggga
     4261 atctgatttt ctttatcagg caaatcagga aatggtatct gctatgcagg aacaaggctt
     4321 ggcggtcacc tttgaacaag gacctggcga tcataactgg atcttctggg accaatggat
     4381 tcaacgtgca ttggcgtggc taatggaaga aaaataaaaa aagagtctta aaaagatagc
     4441 tcctatagag atcacaaaca tagtgatctc tataggagct tttttactgc tcgttttctc
     4501 ttcttttatt ttgttggcat aagccttcaa tataggtttg tgcactcgtt agaaagagtg
     4561 ctttttcatt caattctgca tgggactgca tactttcttt aaatccctct aattgatttt
     4621 caactacata attacgcata aatagaactg tttctttcaa aaattcattt ccgtccataa
     4681 ttttttgtcg aagctgctgt tcatgctgct gatccattaa ctgtccaatc aataaattgt
     4741 ggagagaggc aatggcctga tgactttggg tgactgaaaa tccttcacga acaagtaatt
     4801 ggatcatttg ctccaaatgt tctaatcgac tcggaaaagc gggcacagta ttgatttcta
     4861 attctgcccc acaagggaaa cgtgtataaa ggtcatagta attttccata tactgcagca
     4921 actgttcatt ccaaggcaaa gttggatctg gaagcgctaa ttcttcttcc attgtttcag
     4981 ccattttttg caagagctgt tgtttgtttt tgaaatacca atataaagca ggtgcttgaa
     5041 catgaattct gtttgcgatg tttcgcatgg aaagctgcga aagagcttgt gtttcagcca
     5101 ggatatcaaa ggcggcctga atgatgattg cttgagaaag ctttatttcc attttatcaa
     5161 cctcgtgtat tgcattatct ttttaaacat ggtaaattgt tagtcggtct aattttacag
     5221 tgttaaatta gtcctgtcaa tttaaacaaa ggggtgtaat caacgaatga aattaatctt
     5281 acgacatgcg aaaaaatata aagtagctgt ttttatttcc ttgctgtccg tagcagtcat
     5341 ggttgcagca gcattatggc aacctaagtt actacagcaa gtattggaag ctattattac
     5401 agaagataat gatgaaatga aaacaattgg tatttacctg atcagtctgg ctttgctagg
     5461 attagccgca ggtgtaacga acacgatttt ctctgcaaaa gtagctcaag gcgtaagtgc
     5521 agatatccga gaagaagcat ttcgaaagat ccagacattt tcatttggaa atatcgaaca
     5581 gttctctgct ggaaatcttg ttgttcgtct aacaaatgat atcacgcaaa tccaaaattt
     5641 agtgatgatt tctttgcaat cactttttcg aattccattc ttatttattg gtgccttcat
     5701 tttagctatg atcacgatgc cgcaattatg gtggatcatc gtagcgctta tcgttacggt
     5761 atttctgatt acggcacttt catttacaag gatgggcaag cacttcatga tcattcaaaa
     5821 actgattgat cgtgtgaata gcattgcaaa agaaaactta ctagggattc gtgtagtgaa
     5881 atcttttgta caagaagaaa accaattgaa gtcattcagc aaagtaagtg aggacttaac
     5941 aaaacacaac atcatagtcg gtacattatt ttctgtgatg atcccgtcct tcatgctggc
     6001 tgccaatttg gctgtggttg gggcgatctt ttttgtgagc gatcttgtta aagatgatcc
     6061 tacgttaatt ggtggaattg cttcattcat gaactacttg atgcagatta tgatggcaat
     6121 cattattggc ggaatgatga tgatgatgac ttcaagagca gccgtatcgc tgaagcggat
     6181 tgcggaaatt ttagatgctg aaccagatct tacttattta gacgttccag aacaagagtt
     6241 gacgggttct gtcacttttg accatgtttc ttttcgttat cctggtgagg atacagatac
     6301 gcttaaagat atcagctttt ctatcaaacc tggagaaatg gttggtatcg tcggggcgac
     6361 tggtgccggg aagtcaacac tagcacaatt gatccctcgt ctatttgatc cgacagaagg
     6421 caaagttgaa gtaggtggtg tagatcttcg tcaagtaaat gaaaagagtt taagacaaac
     6481 ggtttccttt gttttgcaaa aagcaatttt attctcagga acgattgctc aaaacttgcg
     6541 ccaagggaaa aagaatgcaa cagaaaaaga gatggaacat gcttcttcta ttgctcaagc
     6601 aaaagaattc atcgaaaagt tatctgatcg ttacgaagca ccaatagaag aacgtagcag
     6661 taacttttct ggtggtcaaa agcaacgttt atccattact cgtggcgtta tcggtaatcc
     6721 aaagatcttg atcctagatg acagtacgag tgcactggat gctcgctctg aaaagctggt
     6781 ccgagaagca ctcgatcgag atttgaaggg aacaacgacg attgtcatcg cacaaaaaat
     6841 tgcttctgtt gtcaaagcag accgcatctt agtattggat gaaggcagat tagtcggcga
     6901 aggaacccat gaggaattgg tagctggaaa ccgtgtctat caggaaatct ttgaaactca
     6961 aaaaggaacg gaggagtaag tatgactgat ctaataaaag caagtaaatt tttctatcac
     7021 tatttgaagc ggtacaaact ctcctttttc tttatcttta tcacagtgat cattgctacc
     7081 tatctacaag taaaggcacc tcaatacatc ggggaagctt ttcaagaatt agctaattac
     7141 gtaggaggct tgatgcaagg cgtagacgat aaaagcaaat ttgtttctgt aatatggaaa
     7201 ctcttgctgt tttatgtact agctagtgca gccaacttta tctatagtat tttgttcaca
     7261 caagtagtag ggaagtcaac gaaccgaatg cgtatcggtc tattcaacaa attggaaaaa
     7321 ttgacgatcc gtttctttga ttctcatcaa gacggtgaaa ttttaagtcg ttttaccagt
     7381 gacttagata atattcaaaa tagtttgaac caagctttgc ttcaagtcat cacgaatgct
     7441 gttttacttg tcgggatctt gatcatgatg ttccgccaaa atgtagagtt agcttgggct
     7501 acaattgctt ccactccagt agccgttttg atagctgtct tgattatccg caaagcacgg
     7561 aaatatgtgg atcttcagca agatgaagtg gggaaactaa atggttatat ggatgaaaaa
     7621 atcagcggac aacgtgtcat catcacaaat ggcttacaag aagaaacgat tgaaggattc
     7681 ttagagcata atcaaagtgt tcgcgaagca accttcaaag gacaagctta ttctggtcta
     7741 ctatttccga tgatgcaagg aatgtcactg gtcaatacag cgatagttat ctttttcggc
     7801 ggctggttag cattaaatgg tgatttggaa cgttcggtgg cattaggctt agttgtgaca
     7861 ttcgtccagt attcacaaca atattaccaa ccactgatgc agatttcatc tggatacagc
     7921 atgatccaat tagcgattac aggggcaaga cgattgaatg aaatatttga tgaaaaagat
     7981 gaagtcaaac cagaaaacgg taaagcattt gatggtgttg aaaaaggtct tgcgttgcag
     8041 cacgtagatt ttggttattc tccagaaaaa ctagtcttga aagatgtctc gatcaatgtg
     8101 gacaaaggag aaatggttgc tcttgttggt ccaacaggat cggggaaaac gacgatcatg
     8161 aatctgctaa accgttttta cgatgtcaat gatggttcag tagtcatcga tggaatagat
     8221 atccgagatt atgatcttga tgcattacgt agacatgtag gaatcgtttt gcaagaatct
     8281 gtattgttct ccggaacgat caaagacaat atcacattcg gcaaaccgga cgcttcagat
     8341 gaagaagtca tcaatgcagc aaaacaagcg aatattcatg acttcatcat gacgttagaa
     8401 aaaggttatg atacagagat atccgaagaa aataacttat tcagtacagg tcaaaagcag
     8461 ttgatcagta ttgctcgaac gatcatcaca aatccatctt tattgatttt ggatgaagca
     8521 acaagtaatg tagacacggt aacagaagca aggatccaaa aagcaatgga tgaagcaatc
     8581 aaaggaagaa ccagctttgt gattgctcac cggctgaaaa caatcttgaa tgcagataga
     8641 atcgttgtct tgcgtgatgg ggaagtcatt gaagaaggca gtcatcatga gttattagca
     8701 gaaaatggat tttatgcgga actttatcat aatcaatttg tatttgaaag ttaaattaaa
     8761 agaacctcgt tataacgggg ttctttttat atattttatg ttgtttgttt tt
//
LOCUS       DAXWLH010000110         8756 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXWLH010000110 DAXWLH010000000
VERSION     DAXWLH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.111"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            107..754
                     /locus_tag="ACGE36_001992"
     CDS             107..754
                     /locus_tag="ACGE36_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384364.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(847..3126)
                     /gene="helD"
                     /locus_tag="ACGE36_001993"
     CDS             complement(847..3126)
                     /gene="helD"
                     /locus_tag="ACGE36_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFF8384365.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKNLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3404..5227
                     /locus_tag="ACGE36_001994"
     CDS             3404..5227
                     /locus_tag="ACGE36_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8384366.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5274..5453
                     /locus_tag="ACGE36_001995"
     CDS             5274..5453
                     /locus_tag="ACGE36_001995"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFF8384367.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5790..7730
                     /gene="thrS"
                     /locus_tag="ACGE36_001996"
     CDS             5790..7730
                     /gene="thrS"
                     /locus_tag="ACGE36_001996"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFF8384368.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7954..>8756
                     /locus_tag="ACGE36_001997"
     CDS             7954..>8756
                     /locus_tag="ACGE36_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8384369.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKV"
BASE COUNT         2747 a         1562 c         1871 g         2576 t
ORIGIN      
        1 ttttttgtgt gatttatttt cttttctaat tcaacctgtg gtatgatgag agcataagta
       61 tcatgtgaaa tgatatgaag tatgtggaag atggacgggg aagaatatgg aagcaaaaat
      121 cactttagaa ccttttgaac gtatcttaag cggttaccgc aaagtagaag agttagctgt
      181 gaacgtaact gattgttcaa aattagcgca aaaatacgca agattcggcg ttgaaggcta
      241 tcgattagga aactatgtcg gtacagggta tctgaaccgt tatttagagt gtatggtgga
      301 ccgcgcacca atgctgattt atagacaaaa ttatctgatt ccgctgcttt ttcgtcgttc
      361 tgattcagcc tttcggttat ttgaagaaga gtatcggatg gaggctttct ttctactatt
      421 agagtggagc ttgaaacatc gtcctgaaaa aattcttatc gaaagaaatg agaagataga
      481 cacgaaaaaa aataaagtga tcgatagtgc atacctagca tttcgtgtat cagagatttt
      541 ggattgtggc ggctatccaa tcagcaattt ccaatcaatt gaccaattca ttgaatggaa
      601 tcgaatttac cgattgatcg acaatggcgg gattggccgt catagcaagg tatttgaccc
      661 agaatatcca gaaaacatgg aagaactgaa aatgattatt tcgcttgtca aattaaaata
      721 tcctgaaaca gatttggatt tatatataga ataaatgacc ttaaatgatt gaattacttt
      781 ttctgcaatt attattgtaa ataaaaagac aactgctaac attaatgata gcagttgtct
      841 ttttacttac agtacttttt tctcataaga tgaaatatcc atattgttta acaaaggact
      901 ttcattattg gaggtaaaaa gggtcaattc atgcatcgca cgtgtacaca tggtgtaaag
      961 gatcaatttg tcttgctgag aagaaaaatg ttctgcatct acatcccaca agaaaactcg
     1021 gtcaaactcc agaccttttg ctaaaaaggc tggaagtaaa ataatggaac gcttcataaa
     1081 gtcactctca tttaaaatca gctggacttg ttgtttcaac tcgtctggca gttgagcata
     1141 aagagcttcg cattcttctt gtgttttagc tatgattgct gtacgccaat accgggcttt
     1201 tccctgagtc gttcgaatgg tatctgctaa ccaattcaat ttgatatcat ctgttgttcc
     1261 ttggatgagc aaaggaaggt ctccttttct tgctgttgtt tctacttgat gttctttcac
     1321 aataaaatga ttagcaaaat cagtgatttc ttttgtggag cgatagcttg tcgttaattg
     1381 gaaacgagtg acttcacggt ccacgaaaag gtcttccaat gatccaacga tcgtttcatt
     1441 tccaaagaca ttttgattta gatctccaca gaaagtcaaa tttgcttttg ggaacaaggc
     1501 ttgcagcaat gccacttgtg atggtgcgaa atcctgcatt tcatcaataa agatatagcg
     1561 agctttgtgt gtaaccgtca ctggatatgt tcggcgcatc agtaaaaaga atagtacagc
     1621 atcctcttga cgcaattctc tttgacgcaa gtgtgtacga acagttgtca aatgttcctg
     1681 ccacctggtc tcgctgattt tatgtcgtgt tagaagagaa agtggcacag tttgtagaaa
     1741 gtggagatat tgtttcgtta tattgacaaa ttgataattc atgatccctc gatggattcg
     1801 acggaatctt ttacgtacga tttggttggc caggttttta cgtaatgaac gctccttttg
     1861 ttctgtatat tcaagatttg gatctgttgc gtatagttct tggagctgtt cttccgccag
     1921 atctttgacc cattgctgac gtgtttcgtc tttttgcaga ccgcctaatt ttttcaggag
     1981 cttcgtttgt aatagagaaa gtctatgatg taaaggtaat tgttcatttg tttctttata
     2041 ccattggcga atcgattcct ttgataggat cgtacggcca tttattttca tatcacggaa
     2101 caagggaccg tagtttgtga tgccttggat atagcgatca atctgatcga ctaaggtcaa
     2161 tccgcttttc atcacttgta tcggatcttt ttcccctagc aaaaaaccac tttcttgttg
     2221 ctgttcatct agtaaagaaa actctggcag cagctgcacc aaataatttt tgaatgtctg
     2281 tgtaggcacg ccgctttcac ctaatgaagg caatacagtc gagatgtaat cagaaaacaa
     2341 atggttgggt gaaaatagaa gaacgttttc agcctcgagc cacttgcgat tatgatacaa
     2401 cagaaaagca atacgttgaa gcaaagcgga tgtctttcca cttccggcga ttccttcaat
     2461 cagcattact ttacttgttg tgtcccgaat gattgcattt tgtgcttttt gtatcgtaga
     2521 gacgatgttt ttcatatggt tgcttgatga ttcatccaat atctctagca aaaagtcatc
     2581 attgattact tccgacgtat ctaccataga taaaatacgt ccttcttgga ttttaaattg
     2641 tcgtttcaac tgcagatcga catctattct atctgtttca gtttcataaa atgcgggacc
     2701 aatctcacct tcataataaa gattggcaat tggtgcgcgc cagtcaataa cgattgtttc
     2761 ttctgccaga tctcttaaag aagcgatacc tagatataat gtttctgttt cacggtcttc
     2821 tttaaagtcg atacgggcaa aataaggact atctttcatg acagacaacg tttgcaggcg
     2881 tttttgctgt gcttcaagag tatggtattt caaaagcaat tcctgctcat gttggcgata
     2941 ttcaacagca gattcataaa aagcttcatt ggaaccagct cgaatcttgt gattagctgt
     3001 gtcttttgtc gaatgattca tttcctgagt caattcttgt tgttttgtat ccagcatatt
     3061 ttgttcgatc tggattagtt cgatcgtttc atccacgtgc gtttgttcta gttgtttctc
     3121 attcatcgca tgtttgctcc ttccgccgta aataacgaat taaaagtata gcatagtttt
     3181 tcgaaaaaag gtacttttta actgatgaat cgaaaaagta atccttgctg aatatttttg
     3241 tttgtagaac tgatgaattt tggaaacgct gatagtgata gttgttttca attaaggcct
     3301 attgttttaa aaagtgctag tcaaggacta tgcttaagat gaaagaggca gctggaaatt
     3361 tattactaac ctctctaagt cacataaaag aggagaaaaa acaatgacac atttcaaaaa
     3421 atttttactt actttgctta ttggtgggat agctttatta agtgaatttg tttttcacca
     3481 gccggccctt gcatttgcga ttgtcgcatt tactggaggg attctcgcct tcttgatgtt
     3541 tttggaaatg atcaagacgt tacgttcagg aagatatggc gtagatgtat tagcgattac
     3601 cgcaatcctt gcaacattag ccgtcaaaga atactgggct agtttgatga tattgatcat
     3661 gcttacgggc ggagatagtc tagaggatta cgcgaataaa cgagcgggta aagaactgca
     3721 gtctttgtta gataacaccc caagaattgc ccatcgattc aaagaaggaa aattgcagga
     3781 tatagtaatc gatgaaataa atattgatga ccagttacta gtcaagcctg gcgaacttgt
     3841 acccgcagat ggtcgagtga ccaaaggaga atcttcggtc gatgaatctt ctttgacagg
     3901 tgagtcccgc ccggaaacca agaaacctgg agatcagatt atgtcagggt ctttaaatgg
     3961 agacggatcg atccaaatga tcgctgaaaa acgtgccgca gacagccagt atcaaatgat
     4021 tattaaacta gtcgaagaat caaaagaaaa gcctgcccga tttgtccgtt tagctgatcg
     4081 ttatgcagtc cctttcacgt tgattgctta tttgatcggt ggtatttctt ggtggttctc
     4141 aaaagaccct ttacgatttg ctgaggtact ggtagtggca tcaccttgtc cactgattct
     4201 agctgcgccc attgctttag ttgcaggaat gagccgttca agtaaaaacg ggatcgttgt
     4261 aaaaactgga acaacgattg aaaagctcgc acgtacaaaa acgattgctt ttgataaaac
     4321 aggaacgctt accaaaggga tattggaagt agaggcgatt cagccagtca atggaataaa
     4381 aaaagaagag ctacttctgt atgcagctag tgcagaacag gaatctagcc atatcttagc
     4441 tcgttcgtta gtgaacaacg tgaaaaacag ccaactgtat cctgttagtc aactgaaaga
     4501 agtgaccggt caaggaatcc aagcagtagt caataatcat gaagtaaaga ttggacgtgc
     4561 ctcttttgtt tcagctgaag aaatagaaac gtccaataca gcaatctatg taacaattga
     4621 tcgatctttt gcaggaacga tcctattttc ggatcagatt cgcccagaag cagcaaagac
     4681 aatcaaggag ctaagaaaac ttgatgtgaa aaatttgatg atgatcaccg gggatggatc
     4741 tgccattgct caaacgattg cagaaaaagt ccaattagat tctgtacatg cccgtagctt
     4801 gcctcaagat aaactgacca ttttagaaag tatccctaaa gatcaaagac ctgttgcgat
     4861 ggtaggagac ggcgtgaatg acgcaccagc acttgctgct gcagatatcg gtattgcaat
     4921 gggggctcat ggttctacag ctgctagtga aagtgcagat gcggtcatct tgaaagatga
     4981 tctcttgcgt gtagcaaaag cagtagtgat ttcaagagat acgatgagaa tagcgaaaca
     5041 gtctgtactg attggcatct ttatttgtat ttttttgatg ttaattgcaa gtacgggact
     5101 aatccccgca ttattcggag ctgttttgca agaagtcgtg gatacggtct ctattttgag
     5161 tgccttgaga gcaaaaaaag atccagccat ttttcctgat tcagtaccca agacaaacat
     5221 agtatgatag aatcgagttg aaaaaagaac gatcagaaag aggagagagc attatgccat
     5281 tcgtacatgt cgaattagta gaaggtcgca gtccggaaca attagaaaat atgatgaaag
     5341 atatcacgga agctgttcat aaaaacacgc aggcaccaaa agaacacatc catgtcatca
     5401 tcaatgagat gaaaaaagga acatacggtg taaatgggga atggaaaaaa taagtttagt
     5461 ttcccacttg atttttctat ttattagtgg ttaaataaac acataattga ttgatgagaa
     5521 agccaactga atttgagagt cttcgataag agaggaaaag ctaggctgaa acttttccag
     5581 gagaaaacca aaggatgacc actttcgaaa cccttgttga agcaagggcg gtcggaccgt
     5641 tacaacgctt gaggaacgca tttgcgtttg aacattaggt ggaaccacga acttttcgtc
     5701 ctagtatctc ttttcaagag atgctaggat ttttttgttt ttcagcttga tgatctgatc
     5761 aatcaaaaca tttcaaggag gaaactatta tgataaaaat tacttttcca gatggtgcag
     5821 tcaaagaatt tgagtctgga acaacaacat tggcaattgc tgaaagcatt tcaaaaagct
     5881 tagcaaaaaa agctttagct ggtaaagtga acggaaaact cgttgatttg actcgtccaa
     5941 tcgaggaaga tgcaagtatt gagatcatca caccagacca tgaagatgca cttggcttag
     6001 tccgtcattc agcagctcat ttgatggccc aagccatgcg tcgcctctat ccaaatatcc
     6061 actttggcgt tggaccagct atcgattcag ggttttacta cgatacagat aatggacaaa
     6121 accaagtaac tgcagaagat ttgccggcaa tcgaagcaga aatgatgaaa atcgtcaaag
     6181 aaaatctgcc gatcgaacgc cgtgttcttt caaaacagga agcattagaa atttttgcaa
     6241 gtgatccgta caaagtagaa ctgattagtg aattgccaga agaagaagtg attactgctt
     6301 atcaacaagg cgaattcatc gatttgtgtc gtggaccaca tgttccatcc actggccgta
     6361 tacaagtatt taaattatta tcagtggcag gtgcttattg gcgtggaaac tcaaacaatc
     6421 aaatgatgca acgtgtatac gggacagcct tttttgacaa gaaagctttg aaagaatata
     6481 tccgtctccg tgaagaagca aaagaaagag accatcgtaa actaggaaaa gaacttgact
     6541 tgttcatggt ttccccagaa gtaggttctg gtttgccatt ctggcttcca aaaggggcaa
     6601 caatccgacg cacgatcgaa cgttatatcg tagacaaaga agttagtcta ggttaccaac
     6661 atgtatatac accaataatg ggagatgttg agttgtacaa aacatctgga cactgggatc
     6721 attatcaaga agacatgttc ccgccaatgg atatgggaga tggcgaaatg cttgttcttc
     6781 gcccaatgaa ctgccctcat cacatgatgg tctacaaaaa cacgatccat tcttatcgtg
     6841 aattaccaat tcgtatcgct gaattaggaa tgatgcatcg ctatgaaaaa tcaggtgctt
     6901 tgtcaggttt acaacgtgta cgagaaatga cgttgaatga tggtcataca tttgttcgtc
     6961 ctgatcaaat caaagatgaa tttaaacgta cgcttgaatt gatggtggca gtatatgctg
     7021 acttcaatat tacagattat cgtttccgct tgagctatcg cgatccaaac aatacagata
     7081 aatattttga tgatgacgca atgtgggaaa aagcccaagc aatgctgaaa gccgctatgg
     7141 atgaattaga actagattac tttgaagcag aaggcgaggc agccttttac ggaccaaaac
     7201 tagatgttca agtcaaaaca gctcttggta cagaagaaac attatcaact atacaattag
     7261 acttcttatt gccagagcgc tttgatttga catatgttgg agaagacggg gaaaatacgc
     7321 atcgtccagt agttatccac cgcggaattg tttctacaat ggaacgtttt gtcgcttatt
     7381 taacagaagt atacaaaggc gcttttccaa catggttggc acctatacaa gcaacaatca
     7441 tccctgtttc tgttgatgca catggcgatt atgcttatga aatcaaagaa cgtttgcaaa
     7501 tgaaggggtt gcgtgtagaa gttgatgatc gtaatgaaaa aatgggctat aagatacgtg
     7561 cctctcaaac acaaaagatc ccttaccaat tagttgttgg agacaaggaa ttggaagatg
     7621 caacagtgaa cgtacgtcgt tatggaagta aagaaacagc tgtagaagat ttgaatatct
     7681 ttattgatgc tatggaagca gaagtcaaaa attacagcag agaaaactaa taggtaaatt
     7741 tcctgtaaaa aagtagtaga tagaaagcaa tccgaacaat agataacagg tattcctcaa
     7801 agacacagat gctcaataga atctgtggga ttgatgggat aagatattct gttgggcgga
     7861 ttgtttttat gttttttatc cagtatacga ccgtgaataa gggtctgata tggtatactt
     7921 acatagacta tcattaaaag ggtggaggaa cttatgagcg taattagtcg tattgaaagt
     7981 atgatggaag attattcttc agcagaaaaa aagttggcca actacattat cggtaatatt
     8041 gagaaggtcc ctacaatgac agctaatgaa ttagcagaag cttccgggtt gagtgcgccg
     8101 acagttgtac gtttttccaa aaaaatcggt tttcaaagtc tgacggactt caaaatcacg
     8161 atttcaacag aactacagac tggaatcgat gaaggtttta gtgatataga agcaaatgag
     8221 tccttttact ctatcaaaaa caaacttggt aacaatgcac aagtagcgat aaaagaaaca
     8281 gtagatattc tagaagaaga aacgatccaa aaagtcgtag agtgtttgga atcggcagag
     8341 actgtttttt tatatggggt tggcgcctct tctttagtag tagaagatat tctgcaaaaa
     8401 tggtctcgtg tcggtaagcc aatcattttt gaaaaagata tccacgtctt gctcccgcaa
     8461 cttgtcagca atgagaaaaa gaaagtgctt tggcttgttt caaactcagg tagaagtgca
     8521 gatgtagtcg cattagccga gctggcgaaa agcatgaata tcgaaatcat tgcgttgacg
     8581 caatttggaa ataacccgct tagcaagata gccgatgtac tcgtacaaac atctagacca
     8641 aaagaaatca cgaatcgaag tgcggcaact aattcacttt tggcccaatt tgctacgatc
     8701 gatatcattt tttatttcta tatggctaaa aacgaaaagc ttagcgaaaa agtgaa
//
LOCUS       DAXWLH010000111         8677 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXWLH010000111 DAXWLH010000000
VERSION     DAXWLH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.112"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            3..302
                     /locus_tag="ACGE36_001998"
     CDS             3..302
                     /locus_tag="ACGE36_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384370.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(399..833)
                     /locus_tag="ACGE36_001999"
     CDS             complement(399..833)
                     /locus_tag="ACGE36_001999"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8384371.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1065..1775)
                     /locus_tag="ACGE36_002000"
     CDS             complement(1065..1775)
                     /locus_tag="ACGE36_002000"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8384372.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1765..2625)
                     /locus_tag="ACGE36_002001"
     CDS             complement(1765..2625)
                     /locus_tag="ACGE36_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFF8384373.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2630..3268)
                     /locus_tag="ACGE36_002002"
     CDS             complement(2630..3268)
                     /locus_tag="ACGE36_002002"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFF8384374.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3283..3582)
                     /locus_tag="ACGE36_002003"
     CDS             complement(3283..3582)
                     /locus_tag="ACGE36_002003"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8384375.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3625..5109)
                     /locus_tag="ACGE36_002004"
     CDS             complement(3625..5109)
                     /locus_tag="ACGE36_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8384376.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5129..5590)
                     /locus_tag="ACGE36_002005"
     CDS             complement(5129..5590)
                     /locus_tag="ACGE36_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384377.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5608..6675)
                     /gene="ulaG"
                     /locus_tag="ACGE36_002006"
     CDS             complement(5608..6675)
                     /gene="ulaG"
                     /locus_tag="ACGE36_002006"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFF8384378.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6893..7663
                     /locus_tag="ACGE36_002007"
     CDS             6893..7663
                     /locus_tag="ACGE36_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8384379.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7936..>8677)
                     /locus_tag="ACGE36_002008"
     CDS             complement(7936..>8677)
                     /locus_tag="ACGE36_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384380.1"
                     /translation="QATVSNIENKNMSPGINILSALCKKLNVEISDILVETEEDKIRK
                     KINEIQSLNSVSKYQESYKILQTIDQEKVSNSDLLNQILFYQGLLDHLYAKKPDAALF
                     YFNQVLEQTTETDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVC
                     IVYYDIATYYRKIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDY
                     LERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2629 a         1827 c         1375 g         2846 t
ORIGIN      
        1 acatggatta tactgttgaa tttgacagtg aactacttaa gcaatttcca caagaagaac
       61 atttcatgta taaagaagca atttcccaca aagtgacaga gtatttggag cataattttt
      121 ttcgtataaa gccagtgcgt actcgttcgg caaaaggaat atacgagatg aaagtcaaaa
      181 ttaataaaaa aaattatcgc atagcatttg ctttaaaaaa aaatcatgtg ttcatttttt
      241 atattagttc gaatttgcaa aaaattgtat ttgataaaga ggtgagtaaa aaaataaaat
      301 agctgagcat tggaggatag attgagtgat actaagtcta tcctcttgtt tttctaaaga
      361 actaagctag aattttttct tttgtacgtt attatatgtt atttgtcatt accaaaaagc
      421 accttttttc ttctagtatc tatgacataa gaaataccac ccatttcctt tacttgaaca
      481 aatcctactg attcataaaa tttttttgtt ttctcagtat catctgtcaa taattcgatt
      541 tggactggtc cataactagt gatcttctcc aaagcttcat tcaaaagcac tttgcctatt
      601 ccttggcgct gatacctcgg atgaatgagg atatcttgaa taaatgcgat cgtcgcaaaa
      661 tcagtgatcc aacgaacgat tccgataatc ttcccttccc cattacgctt tatcagagat
      721 tcagattgtt caaatgctct agctaacctt gcagtgtctt ttgtgtaatt cgtccaacca
      781 acgctcttat ataattcaga aatctcatcg atcataaggg aatcaatatt catttttttc
      841 ttcctttctt atacaattct ccctctaata atacagttct ttgactattt aatctatcac
      901 atataactct cctgtaaaga ttctctcgat tccttaaaat tcctctttcc ttttagaatg
      961 catacttttt gataaataaa aaggttgtga taaattttct tcacaacctc tgtttctctc
     1021 tatcaagtgt tatcttttaa ttttctaccc ttaccctagc atctctattg cccgtagtat
     1081 gcatactttc catgtttgcg aaaataatgt ttatctagta aatattgcgg gatttcacat
     1141 atattggggt tgatcagctc gttttctttg gccatcagac aaatttcatc taagatcaaa
     1201 ctgttttcta ctgctttcat aggtgaatcc ccccatgtga acggtccatg tccatagaca
     1261 agtactccag ggacttcgtt tggatctaat tttctttcct taaacgtctc gacaatcaca
     1321 ttccctgtat gaacttcgta agcttctctt acttcttctt tcgttaattg gcgtgtacaa
     1381 ggaaccttac cataaaaagc atcagcatga gtcgtgccat aagctggcaa atcgcgtcct
     1441 gcttgcgccc acatgacgga gtgcgtggaa tgagtatgag taatagcagt tatatcttca
     1501 aaagtttgat acaaaacaac atgtgtgggg agatctgaag atggttttaa gctatcttct
     1561 tctattatat ttcccgacaa atctgttacc accatttgat ctgcttgcat gcattcatat
     1621 ctgacaccgc ttggcttaat gacgatgact cctagctcac ggtccacttc actcacattg
     1681 ccccaagtca atttgactaa tccgtatcgc ggcaatgcca gatttgcttc aaggacacgt
     1741 tgcttcattg ctgcaatttt ttcttcacaa gacatcatag ccagcctcct tcaatttaga
     1801 acttaagtag tcttttgcct gctggatttc tcttcgaaag tcttggcttt tttcactcca
     1861 catttcaatc agaaatgttc catcataatc tagacgtttc aaatgtctta ataatccaag
     1921 aaaatcaaca caaccgctac caaaaggaac atcacgaaac tggcctgaat attcttttgt
     1981 tacagggtaa gtatctttta agtggatagc agtgatataa tcgatccctt tctcaagttc
     2041 tcgagccgga ttattttccg gccatgcgga caaattgcct aggtctggat atacttgtag
     2101 aaaaggtgaa tgaatctgtt ctttgatttc taaaaatttt gaaatggagt tcataaatgg
     2161 atcatccatg atttccacgg aaagaatgat gccatattta gaagcttcct ttacgctttc
     2221 ttttattcct tcgataaagt cttctctgga attcattgat ttctcttcat aataaacatc
     2281 gtatccagct atttggatta ctttgacgct tagattatgc gccaaatgga ttgctttttt
     2341 cattagttgt ttcgctgctt ttttcttttc tggatcagca gaaccaaacg gaaatcgccg
     2401 atgcgcactt aaacaaatcg aatggatacg tacaccggag ctgaacatct cttctctcag
     2461 ctcagctatc ttttcttcag accaatctaa tcgtgctaac cgctcatccg tctcatcaat
     2521 cgacatctcg ataaaatcga agtccatttc ttttacaagg gaaaaacgtt ctttccatga
     2581 aatatctttg ggtaacgctt tttcataaat cccaatttga gccatttcct tacccccaga
     2641 tacgaatgat ctcatctttg aagttttgtg cggccttcat cggatcattt gctgctgtaa
     2701 taccacgacc agtgataaag gtatacacat cgatcccttc aaataagtgt aatgtgtgtg
     2761 gatttaatcc accagttaca gaaacattaa agcctagttc aatcagtttt ttgatcttcg
     2821 ataaatcttt ttcaccccaa gtttcaccag ataacagcgc atcacggctt tgatggtaga
     2881 ttacctgccg gataccgatg ttatgccaat ccatggcttg ttcaaaagtc caattgccgt
     2941 agagttctac ttgcaattca cctatttctt tttgtgctgc ctccatagtt ggaatcgttg
     3001 cacaacaaat gactgtcatc cagtctgctc ctgcttttgc aacattactc gccactgttc
     3061 ctccagcgtc tgcacatttc gtatcagcga ctagtttttt atctgggaac atactgcgaa
     3121 tacagcgaat cgctttttgt ccttcctgta agcaaagaat tgtgcccact tcaataatat
     3181 ctactacatc accaactttc ataacatctc ctaatgcatg ttctaagtca ttgtgatcta
     3241 acgcaatttg caaattcggc ctgctcatct tagttccttc ctttacttgt ttttttggta
     3301 ccactttgtt tcagcaaatt tttcactgat ttctttgact gacatcacat ttttgatacc
     3361 gataactgta actccttttt tctctgcctg agtaaacata ttcaaaaaat tgactggcgt
     3421 aaaaactaca tcgtattggc cagctgaact ttttccctct gaaatggcac aatgatggat
     3481 tttggtaatt ggtacgttta gttctttcat tgctttttcc acacttctca tcatcatcaa
     3541 gcttgttcct gatccatttg cacacgatac taaaattctc atcttaatcc ctccattatg
     3601 agtgttgtcg aatcagattt tcttttatac tctctgcttt tctgcatatg tgtcatagtc
     3661 ttctacaatc aaaaaataac catcaggatt tttccgatat tgtaactgcg gaatagccaa
     3721 taaaccaatg acaactacgg caattccagc ataacctagg actttcatga ttactgtaaa
     3781 caatggccaa gctgttgccc agtcgaacat cccgatataa ccaccgtatg ttgacatccc
     3841 aatccatgta gcgaataatg ctgaaccgcc tacttgtatg attcctgaaa gaaacgggaa
     3901 caggcaagcg gctttgaatc cacctcggtt attggcataa actgctataa cggcattatc
     3961 aaagaacaac ggaatgaaac cggctatgac gatcgttggt gatttcaata aaatcagtaa
     4021 accaatcatc aaaaactgtc ccaaagcacc aaacaagaac ccaatcgtta ccgcatttgg
     4081 cgaaccaaag ccaaaagtag cagctacgtc aattcctgga acagctcctg gtaaaatcgt
     4141 gtttgagatt ccttggaaag attgagttag ttcatcgacg aatgtccgaa cacctaattg
     4201 caagatcgcc agatataccg caaaatttaa agaagtcgtc ataatgtaga ataagaaact
     4261 ctgcccttct tgcatgaatt gcgcttgaat caaataatct ttgcctagta caatcagaat
     4321 gatcccaaag aaaaatagca tgagtaacga agtagcaacc atattctcat tgaaaatcga
     4381 caagaatcct ggtaatttga tatcttcaat ttttttgtct tgcttgattg atctattttt
     4441 cttttcatcc cgctttttca tccactcagc taattttgca aatataaaga ttccgaacat
     4501 ttgctgatgt gccactgcaa atccggcacc ttctgtcaat tcctgcgtga ttcctactgt
     4561 taagttagaa gctactgtcc aataacaacc taaaatcagt cccataaaaa tcaatgcttg
     4621 tatcctacct agatcaggaa agcaaaacag taaaatccaa aaagcggtag ctgcttgttg
     4681 aacctgaacg tttccggtag tgaaaacgga acgcagtttc gtatatttct gaaaacggac
     4741 taataagata tttaaaacaa atgcaatcag taataaaatc atcacatctc caaaggtacg
     4801 gccaaatgtc tccattaatc cagcatccac agcattttgt ccaaaataag gatctgtaac
     4861 catggcatcc aaattgaacc gttcctttag tccaaccaag attggccgaa aattattgac
     4921 caaaccacca gagccaacag tcaaaataaa gtaaccgacc gttgccttta agaatccagc
     4981 gatgcattca tgaagcgggc gtttcagcaa aatatagcct aataacacga taaaaccaat
     5041 caggtatgct ggctgcgtca agatatttgt tgcaaaatac gtccaaatgc ttaacaaaaa
     5101 ttcccccatc ttttttctcc tctatcaatt aattatattc tgccatcaca acttggtaat
     5161 catcgatgga gttcgtagag atgagacgct caaccaatcc atctgtcatt aataattcgg
     5221 ataatttttg gatattttct agatgttctt ctggatcttt tgctgcgagt gtaaaaaaca
     5281 aactagccgt tttctcctca ttctcccctt cactttcaaa acggattggc tgtttcaact
     5341 tggtaaatga aatagctgtt ccaaaaacac catcactttt ttctgaagaa tgcggcattg
     5401 ctacttgagg tgcgatcaca atataaggac cgtatttttc tacacaatgg atgatctcct
     5461 ccacatacgt ttcatcaata atttccttat cgatcaatac aagacagctt tcggcgatag
     5521 cttcacgcca atccgtgatt tctttatctg ggtaatgaat caattgatgg tcaaaaaagt
     5581 atttcagcaa gaaactcccc ttctttctta taaaaacgac ggaaacggtg tatcattttc
     5641 aatcgagaag acatcttcaa atcctcgata aaaattaaac tcaagatcat ctttgtttgt
     5701 cggatagatg aatttcccgc ctacttgcca gatataaggt ttaaatccat acttcaggcg
     5761 atcctttttg aatttccaaa tttcagtgat ctcttttgga tcagccataa aattagacca
     5821 aatatcgtag tggacaggaa tcactacttt tgcattcaat gactccgcca tgcgcagcat
     5881 atcaacagaa gtaactttgt ctgttatccc tcgtgggttt tcaccatagg cccctaaaca
     5941 gacatcgatt ttgtgttcat taccatgttt ggcaaataaa ttggagtaat gtgaatctcc
     6001 tgcatgataa agattccctc ctgatgtttc gaataaatag tttactgcaa tttgatccat
     6061 ctcctgaggc atttttcctt ttaatacctc ttctggatct tcacaagtga ccaacgctgt
     6121 ccgatcaaac gcttctaaag ctactacatt gatgtctttg attttgactg catcacctgg
     6181 ctttactgta atcgtttttt cttcaggaat tccccatttt tgccacgtgc gaaccacttc
     6241 ttttggccca atgaatgttg cttcaggaca attttgatga actgctgcgg ctgtatggat
     6301 atctagatga tcggaatgga tatgtgtaac gattaaagcg tctacatttt ttatcgcaaa
     6361 aggatcgatt acgaaaggct gagtacgtaa attaggctgc atattttgaa cgccactcat
     6421 acgcatcatc tgatggcctt ttttcatcat gccattccca tgactacgtt ttcccgtccc
     6481 acaccataga tcacataaaa tattcgttcc ttcatgactt ttcagccaaa ttcccgtaca
     6541 gcccagccac cacatagcaa aagttccagg atccactact tctgtttcga tttcttcatt
     6601 caagtatgtg ccccactcag gaaatgtttc tactaaccac ttttcttttg ttacttcatt
     6661 gacagtttta ctcatttgct tgctcccccc atatcgttgt tccaacttac aagtaaatat
     6721 taatgtgtat cagcatccat gaaaaccctt ttatatgacc gtttgttttt tatatgattt
     6781 tctttcagta atcaatgatt gtttcagtat aataaagaca gatatgaatg tttaaatgaa
     6841 caactagtca aataaatcca tggatcaaga tgtccctcag gaggcaaaag tcatgaaaaa
     6901 ctcacttgtc aatattgaaa aacgtcaaaa agaaattttg gaactgcttc agcagactga
     6961 acaactatca actttcgaat tagctgaatc ccttgatgta tctattagta cgatacgaag
     7021 agatttaaat ttattagaag aaaaaaacga gatcatacgc aattacggtt attgttctta
     7081 taattacaaa aaccagactg attttgatca atctggtcct gaaaggatta aacaagcgat
     7141 tgccagagaa gctagtaaat acattaacga ttatgatacg ctgtttatca attctagctc
     7201 gactgcgttg aaaacactgg attttcttca atctaaccat ttgacagtca ttacaaacaa
     7261 cgtgaaaatc aattactctc cgcacaaaga taattgtagt tatattttaa ctggtggaga
     7321 gatgcgcttt ccaaaagaag cactagtggg cgacatcgca ttaaatacga tttcttcagt
     7381 caatgcagac atttgtatca ttggctgtag cggagtcgat gtagtacatg gggtcacaac
     7441 aaagatatta aatgaatcaa cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa
     7501 gattttagtc gctgatcatc gaaaaatcgg actgacatca aagttcaaga ttgctgatat
     7561 ttcagtcttt gattatttga tcactgataa attttgttct gaaaaagtat tagaggagat
     7621 cagaaatacg gggataaaaa ccattcaagt agattcattt taagaaccag gacgtaaata
     7681 tgtaaatagg attatcaaaa aaatgaataa caaatttatc gataaatgaa aaacacgcta
     7741 cgctcaaatt gataaaggaa tcccaagatg tatccatcca atcgatacca tcataatcta
     7801 ttgcttcttt tataaccgtt atatgttgcc taaataggaa tcaaacaata tcgaaaaaag
     7861 gaggtatgac aaaagtcaca cctccttttt tcgaataaac ggtattcaaa tcagaacact
     7921 gatttcacaa ccactttatt tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc
     7981 gttgaaacgc gctatttttt ttgcgtccat atatctttct aaataatcat cttctcctaa
     8041 ttgcttatgg caagaagcaa tttcataaag caagtattcc aatgcataag tcgatttatg
     8101 tttcttatta tactcaatac ccttctcaca taattggatt gctttatcat aatcctcgat
     8161 tttacggtaa taggtggcga tatcataata tacaatgcag accatcagat tatcaaaatc
     8221 agtctcactc aatattttaa gtgtacgctc cacataaact ttcgcaaaat ctaattctcc
     8281 ttttgacaag tagatcattg ccacgtttgc agctgcttgt aagtgatgga tatctgtttc
     8341 ggtcgtttgt tccaacactt gattaaaata aaataatgct gcatctggtt ttttcgcata
     8401 aagatgatca agcagtcctt gataaaataa aatttgattt aaaagatcag aattacttac
     8461 tttttcctga tcgatcgttt gtaaaatttt ataagattct tggtattttg atacagaatt
     8521 caatgattga atctcattta tttttttcct gattttgtct tcttctgttt ctactaatat
     8581 atcggagatc tcgacattca gctttttgca aagcgcactt aagatattga tgcctggcga
     8641 catattcttg ttttcgatat tgctgacagt tgcttgt
//
LOCUS       DAXWLH010000112         8622 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXWLH010000112 DAXWLH010000000
VERSION     DAXWLH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.113"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(63..1106)
                     /locus_tag="ACGE36_002009"
     CDS             complement(63..1106)
                     /locus_tag="ACGE36_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFF8384381.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1483..2448)
                     /locus_tag="ACGE36_002010"
     CDS             complement(1483..2448)
                     /locus_tag="ACGE36_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFF8384382.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2682..3527
                     /locus_tag="ACGE36_002011"
     CDS             2682..3527
                     /locus_tag="ACGE36_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8384383.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3718..4905
                     /locus_tag="ACGE36_002012"
     CDS             3718..4905
                     /locus_tag="ACGE36_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8384384.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4911..5267
                     /locus_tag="ACGE36_002013"
     CDS             4911..5267
                     /locus_tag="ACGE36_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFF8384385.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5269..5607
                     /locus_tag="ACGE36_002014"
     CDS             5269..5607
                     /locus_tag="ACGE36_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFF8384386.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5683..5939
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6002..7036
                     /locus_tag="ACGE36_002015"
     CDS             6002..7036
                     /locus_tag="ACGE36_002015"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8384387.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7029..7715
                     /locus_tag="ACGE36_002016"
     CDS             7029..7715
                     /locus_tag="ACGE36_002016"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8384388.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7739..8587
                     /locus_tag="ACGE36_002017"
     CDS             7739..8587
                     /locus_tag="ACGE36_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8384389.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2871 a         1571 c         1843 g         2337 t
ORIGIN      
        1 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
       61 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
      121 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
      181 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
      241 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
      301 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
      361 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
      421 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
      481 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
      541 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
      601 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
      661 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
      721 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
      781 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
      841 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
      901 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
      961 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     1021 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     1081 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     1141 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     1201 aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc ttctacggaa
     1261 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
     1321 gaagctgacc acttctttta cgacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
     1381 gtaataagcc caaccagtac aaaaacatga acagctgttt ttgtttggcc gttcttatta
     1441 cttgatgcag aacagctttt tcacaaccta ttgaatcgat tattaagcga tttttttaga
     1501 aacaaatttt ccatgtttgc tatgttcttt aattaagaaa cgtacgtaat gagcaagaac
     1561 gtagaagaca actacacttg caaacagaca ttccgcaact tttaagaaat ccaatacgct
     1621 actatagata cctaaactgt tgaagtaatg aacagtagta aatatagctg ttgcagagat
     1681 aacaagcggg aatgtaaagg ctgcatagct tggataaaaa cgaagcttga acaaacgtgg
     1741 aagcatagac agcacaacga aatacaatac ttgagataaa gcgaacaatg ctacgacaag
     1801 ataaaaatta ggttcagcaa agtttttaag ataacctgtt aggcaaagag aaccaggtgc
     1861 tgcaacgatc gtaatcagcg gacgtgtcgg atgagccata tttcttacga aaaagacacg
     1921 tttgatgatg atcggcaata agaacgcata gcagatcaat gaaatccaaa agaaaatttt
     1981 tccaataagt gggaagaagt tcccggcagt tacagtgatg accccaaatc caacgtaaat
     2041 gataaaccag cttggataaa ttgcttcaat tgaaagatca gctttgataa catggtgata
     2101 attgaaataa aagacaagga tcacttggaa gataacagcg cacagccaaa tgaatttgac
     2161 aattggtgca atactttcat atgaaacaaa ataagtacaa ataaccatga gtgacatcgt
     2221 aaaagttggt gaaacggagg cgatgattgg atcatgcaac gtttgtttcg tgtgttcgaa
     2281 taagataaac aatttaacaa aaatgaatat cataagaatc atagagatac cgccagttag
     2341 attgcctaaa gcaacatggt gatagtcttt aaacagattt ccaagggaag ctaacccaag
     2401 gatcaacccg cagatcggga tcggaataat tttcaaaaag ttcttcatat ataatattct
     2461 cctttttatt taatagattt tagtaccaag atgaacgaat acattgtaaa atgaatagga
     2521 atgtatgatt acgagcaagc agacagctga ttgtcttcat ctaagttact agttggatca
     2581 gcaactgttt tgttgtacat ttctattata gatggttttt tgtatattta aatcaaatag
     2641 tttttatagt aattatcata aaaagttatg gaagtggtgc catgtttaag ttattaacga
     2701 cttttcgagc agtatatgaa acaagaaatt tttccagagc agcagaagta ctgtttttat
     2761 ctcaaccagc agtctcgaat caaatcaaac agttggagcg ggaattgaat atcgatcttt
     2821 ttcaacgtaa cggaagaaaa gagatgcttc caactaagca agccgactta ttatatgaac
     2881 ggacactggt tttgttagaa gaatgggaag aaactcagca aaaattgctc aatgaacaaa
     2941 atgaagaaga agtatgtcgg atcgttgctt cccacacctt tgctgtctat cttttgcctc
     3001 agttgatgaa acatctgatc aaaaggtttc ctaaagtgca ttttgagatc gaaatcacga
     3061 attctcatga atcattagaa caagtagcga aacatgaatc cgattttggt tttatcgaaa
     3121 agccattaga gacttccggc gtacaaagat actctttgat ggaagaccag ctagtcatcg
     3181 ctggggaccc aaaaagcaaa ttgtggcttg taagagaagc aaactctggt gtttttcatt
     3241 acacgcagcg ttattttgaa gagatgaata tccaagataa gaagatgatc gtgaaaagta
     3301 acgatgtaat tgttgcgttg ctgaaggaag gaatcggtcg ctcgattttg tctaagcgct
     3361 ctgttccaga aaatgtgcca atgactccgt tgaatttcca tcgatatttt tatttcatcc
     3421 aacgaaccaa tctggtttct gatcagctgc tagagattgc cgaagagatc cttcgataca
     3481 atgaaagcac agaaacttct cacagcgagg agaatctcag ctggtagata attgcatgta
     3541 tcaaaaaaag ttctatgata cactaaagat aagcagtagg cttttgtact gcatttgctc
     3601 aagcaactgc acaaacggaa agttaatttt tcccaaatgt ctatggaaag actttccatt
     3661 atctaactgg accgctataa taatcagcag tatggttttt aagaaaaggg atgaaacatg
     3721 gaaaaaaaat taaaagcttc gaacgataat cgtattgatt atggcgtgat tttaccagtc
     3781 tttctgttat gtttgattgg tctgctctca ttatatgtag cacttagcca tgatccgaac
     3841 aatccagcag tcttgaaagg cgtagggatg caagttgtct ggtatttagt tggagcggca
     3901 gcaatcgttg tcatcatgca tttgaattcc aaatggatct ggaaactgac tccttacctt
     3961 tatggtttgg ggttagtggt aatgggactc ttgctcaaat tctatgatca gaatttagct
     4021 gcttcaacag ggtctaaaaa ctggtttcga ttcggtgcgt ttactttcca gccagcagag
     4081 ttgatgaaga ttgcgtacat cttgatgatg gcactgatcg taactaaaca taatacgcaa
     4141 gtaaaagaac ggacgatgaa atcggacttt tggctgattg ggaagctctt gatcgtgaca
     4201 ataccggttt tagcattgat catggcccaa gatgactttg gaacgatgct tgtctttctt
     4261 gcgatctttg gcgggatttt cctgatgtct ggaatctctt ggcgcatcat cgcacctgta
     4321 gtaatcttgg cagtagtcgt tggagcagga acgatcttct tggttacgac tgaaggcgga
     4381 agagacttgc tttataaagt tggattcaaa tcttatcagt ttgcacgtat tgattcatgg
     4441 ctggaccctt tccatgatac gtcaggaatg agttatcagc cggcccaagg acttttggca
     4501 attggtaccg gtggcttgtt tggtaaagga ttcaacgtaa gcaatatcta tgtgcctgtt
     4561 cgtgagtccg atatgatttt taccgttatc ggtgaaaact tcggctttat cggtggtgca
     4621 ttcgtcattt tcttgtattt tattttaatc tatcgtatga ttcgggtgtg tttcgatacg
     4681 aataatgaat tttacgccta tatcgcaagc ggtttgatca tgatgctgct gttccatgtc
     4741 tttgagaata ttggagcaaa tatcggatta cttcctttaa cagggatccc attgcctttc
     4801 attagtcagg gaggatcctc gattttaggg aacatgatcg gtatcgggtt gatattatcg
     4861 atgagatacc aaaatgaagc acctgcaaga tcttcaagga ggtaagaaaa ttgtacacct
     4921 tttactggta cccgaaatgt tcaacatgca aaaaagccaa agcgtggtta gaccagcatg
     4981 gcgttagtta tcaaaccgtt gatatgatca aagaagtacc gaaggcctca acgattgaga
     5041 cttggctaga aacgagtcaa gtaccaatcc gtcgcctgtt caacacaagc gggatgaaat
     5101 atcgggaact tggactgaaa gatcaagtag gcgagtttac ggcttcaaaa gcgagccaag
     5161 tcttatcgac agatggtatg ctgatcaagc gaccactgat cttacgagag gaccagcttg
     5221 ttgcaattgg attcaatgaa aaaatttatg aaggagaact gatataaaat ggcaggcaag
     5281 catttgaaga aaaaggacaa cctttggatc ttatttaatg gaaaagaata ctgtgttggt
     5341 ttgacaaatg aagcgcaaga agaacttgga gacatcacgt ttgccaatct gccaaaagca
     5401 ggacaatcgc taaaaaaaga tgaacctctg atcgaagttg aagcggaaaa agcggtcaat
     5461 gaatttgcca gcccgctaac gggtacagtt tcttctgtta atgataaaat cggtgaagat
     5521 atcaatatct tgaatgattc agatgaaatg aatgcgtgga tcttaagttt caaagatgtt
     5581 gatccaaaag aatttgacga attataaaat cagaaaatca cttgaaaaaa gttgacactg
     5641 attcttccag ttgctataat cataagcaat cctatatgaa acgagaaagc tttgaaaaga
     5701 agagtacatg tcacggacgt ttgaagagag ccctgtttgc tgaaaatggg taacgaaaat
     5761 gagatggaag atggtcttag agcagagtaa gcgagcgtaa gcaaacttac tacgggagtg
     5821 cccgttacag cgcgacagta tacggaaaga agctaagtcc aagtactgga agaggctgtt
     5881 attgcgaagt aacggtaaat caaggtggta acacggaagt tcaagctttc gtccttttgc
     5941 ttaaggcaag agggcgaagg cttttttgtt tgaaaaatca taagagaaag atgtgaatgg
     6001 catgtcatta atccaactaa cgaatgtcaa aaagacattc aatgggaaaa acggaaacat
     6061 ccaagccgtc aatgacgttt cactaaacgt tgaaaaaggg gatatttatg gaatcgtcgg
     6121 ctattctggc gcaggaaaaa gtacattagt ccgtctcctg aatggacttg agcttcccac
     6181 gagcggcgaa gtgatcgtaa acgatcaaga tattacaaaa ttaaaaaaca aagaattacg
     6241 tacattccga aagaaaatcg gtatgatctt ccagcatttc aacctgttat ggtcaagaac
     6301 tgtacttgaa aatatccagc tgccgctgga attagctggc gtaccaaaga gcaaacgtaa
     6361 agaacgggca gaagaactgt tgcgtttagt cgggttagaa ggacgcggac aagcatatcc
     6421 ttcacaactt tccggcggac agaaacaacg cgtcggtatc gcacgagcat tggcaaatga
     6481 tcctgagatc ctgctttgtg atgaggctac tagcgcattg gacccgcaaa caacagaaga
     6541 agtattagac ttattgttag cgatcaataa gaaattgaat ttgaccatcg tcttgatcac
     6601 acatgaaatg aatgtgatcc gcaaaatctg caacaaagtc gctgtgatgg agttggggaa
     6661 agtcgtagaa gaaggagacg tattgactgt cttcagacaa ccgaaaaaag aagtaaccaa
     6721 acgctttgta caacaagaca tcgaaccaga agctgattcg acagaactat tagctgaact
     6781 catcaaagaa aatccaagcg gccgtttggc aactttgacc tttagcgaaa gaaatgccaa
     6841 cgagccagtc atttcccaag cgattcgacg ctataacatc gatgtcaacg ttgtgtatgg
     6901 aaagatcaaa caagtgaaag aaggttcctt tggttcattg actgtcttga tgactggtga
     6961 aaccaaagaa ttagatcaag cagaagtgtt catcaaagaa caaggagtag gaattgaggt
     7021 gatccatcgt ggataaaagc ttttcagaaa catatctaga ttttaaacaa atcaacccgg
     7081 aaacgatgaa tcaggctatc cttgatacac tatatatgac attaatctca atggtcttag
     7141 tgacgatcat cggattagtt ttgggattga tcctttactc tattggacgc aaaaacaaac
     7201 cgtatgctcg aatcatttat ggaatcgtat cgatcatcag taatattttc cggtcaacgc
     7261 catttatgat cctgatggtt ttgatcattc catttactaa agcaatcgta ggcactatgc
     7321 taggtgcgca agcagccatt ccagctcttg ttttatccgc agcaccattt tatgcccgtc
     7381 tagtagagat tgcttttcgt gaagtcagcc ctggtgtact ggaagcagca gatgcaatgg
     7441 gagcaagtta ctgggaaatc atctggaagg tcttgatacc tgaaagtatt cccgcattga
     7501 tttctggatt aacagttaca acgatctcga tgatcggatt tactgcgatg gccggcgcga
     7561 tcggcgcagg tggacttgga ggtcttgctt ggcaggaagg ctaccagcgt ggtaatctaa
     7621 cagtgacatt cgttgcgact ttaattattt tactaatcgt atttatcgtt caaggaatcg
     7681 gtgatttctt aactaagaaa acagacaaaa gataatagga tataggggga tgtaggatat
     7741 gaaaaagaaa atttttggat ttgcagcagc gcttttgctg actgtaggat tagcagcatg
     7801 cggtaacaac agcgacagca aagattcagc aaataaagca gacgacacga cattgaaagt
     7861 cggcgcttca ccgacaccgc acgcagagat tttggaacat gtgaaaccat tattgaaaga
     7921 tgaaggaatc gatcttgaaa tcgtcaaatt cgatgactat gtattaccga accaatcttt
     7981 ggaagaaggc gacattgatg ccaattactt ccagcatatt ccttatttga acaaagaaat
     8041 caaagaaaaa ggatatgact ttgtcaatgc cggtgctgtc cacatcgaac caatgggctt
     8101 gtactctaaa aaagtcaaag atgtttccga attaaaagat ggtgcaacaa tcattacttc
     8161 aaattctgaa tcagactggg gacgcatcat cacaattcta caagatgctg gcttagtcaa
     8221 agtcaaagat ggagtagatc tggaaacagc aacattcgat gatattgaag aaaaccctaa
     8281 gaacttgaag ttcaaccata cgatcgaccc atcactttta gcttctacgt accaaaatga
     8341 tgaaggtgac ctagttgcaa tcaacgcaaa cttcgcttac ggcgcagggt tgaatccatt
     8401 agaagatgcc gtattgcttg aaaaggacaa ctctccttac gtgaatatta ttgcgactcg
     8461 taaaggtgat gaagacagtg acaaaatcaa aaaactgatt gaagtgcttc accgcaaaga
     8521 agtccaagaa tggatcacag acaaatgggg cggctctgtt aaaccagtaa aggctgatgc
     8581 aaaataaggt atggaacaaa aaagatgaga aataagtctg ta
//
LOCUS       DAXWLH010000113         8487 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXWLH010000113 DAXWLH010000000
VERSION     DAXWLH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.114"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(176..853)
                     /gene="nth"
                     /locus_tag="ACGE36_002018"
     CDS             complement(176..853)
                     /gene="nth"
                     /locus_tag="ACGE36_002018"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFF8384390.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(860..1558)
                     /locus_tag="ACGE36_002019"
     CDS             complement(860..1558)
                     /locus_tag="ACGE36_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8384391.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1826..3010
                     /locus_tag="ACGE36_002020"
     CDS             1826..3010
                     /locus_tag="ACGE36_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8384392.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3016..3459)
                     /locus_tag="ACGE36_002021"
     CDS             complement(3016..3459)
                     /locus_tag="ACGE36_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8384393.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIRDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3685..3972)
                     /locus_tag="ACGE36_002022"
     CDS             complement(3685..3972)
                     /locus_tag="ACGE36_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8384394.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4143..5495)
                     /locus_tag="ACGE36_002023"
     CDS             complement(4143..5495)
                     /locus_tag="ACGE36_002023"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFF8384395.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5768..6214)
                     /locus_tag="ACGE36_002024"
     CDS             complement(5768..6214)
                     /locus_tag="ACGE36_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8384396.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6450..7376)
                     /gene="cysK"
                     /locus_tag="ACGE36_002025"
     CDS             complement(6450..7376)
                     /gene="cysK"
                     /locus_tag="ACGE36_002025"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFF8384397.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7516..8403)
                     /locus_tag="ACGE36_002026"
     CDS             complement(7516..8403)
                     /locus_tag="ACGE36_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8384398.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2310 a         1747 c         1407 g         3023 t
ORIGIN      
        1 attttcccaa taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca
       61 tcctagctac tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt
      121 ttctccataa taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca
      181 ggcgtggttt cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac
      241 atattgatag caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac
      301 caaaaaaaat cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca
      361 gtgtttcttc tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga
      421 cacgttccac gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga
      481 catttgctgt ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca
      541 cttgcccatc gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat
      601 ttcgatacaa accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta
      661 acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat
      721 ctgtcgcctg ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga
      781 gttctccatg tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt
      841 gtttgcttaa catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt
      901 cttcttgttg agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt
      961 ggtcttcttc cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc
     1021 ggtcaacgta attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca
     1081 aaatttccgg ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg
     1141 aagaaagcgg gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt
     1201 cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat
     1261 agatggggta aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct
     1321 tgataaatcc atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc
     1381 ccatatccat agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat
     1441 gcaactgtag aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa
     1501 gtaaaagatt agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga
     1561 tttctcctcc gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc
     1621 ttttctattt acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg
     1681 ttcatgtttt tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg
     1741 ttccttagaa tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa
     1801 cattattgca taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt
     1861 tggatacgtc tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc
     1921 gtttttaacg ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct
     1981 catgtctgtt tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga
     2041 tcattttggg cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt
     2101 gctttttggt ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt
     2161 gacggcagga gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga
     2221 aagaaccaaa gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc
     2281 tattataggg ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact
     2341 ctttactttc ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa
     2401 aaaacgttca gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt
     2461 attcaagatt tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc
     2521 tggctctctt caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg
     2581 gttggttggt ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat
     2641 tatgcctcga ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag
     2701 tgaaatcctc gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt
     2761 tttatttggg attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg
     2821 ccaactatcc aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag
     2881 tatacaatct ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc
     2941 accatttctt ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa
     3001 taaaaaatag tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg
     3061 aaatactgtg tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac
     3121 ccatttttga tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg
     3181 aaaatagatt tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat
     3241 cagttttttt gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt
     3301 tgtcacgtta ggatcacgga ttttcccgat atgttcaatc acttctagtt catttagagt
     3361 aaatcctgca aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa
     3421 atccagatat tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt
     3481 cattatattg tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc
     3541 aatctgaaac caagaaaaaa aggggttgac aaagcttgtc ataacccctt tttctgatta
     3601 aaagatagta aagagacagt catcggtaat tgtgatgatt ttttgtcatc acttaaggat
     3661 gagcggcctt ttcaaaaact gtctttaaag atcatcatca gtttctagca catatggctg
     3721 accttcatat gtgtactctg tattcaatgt gccgtcattt tcatctgagt agatgcgtct
     3781 actgaaagga aaacgatgag aaacattatc tttggcgtca tttgctggca tcgtgtaata
     3841 ttcctcttgc gtttcattca actgagagag tacttgatct gccaactcat caatattgat
     3901 cgttaatttc ttcgaagcac ttttataatc ttctaaaaat gcatcttgtt ggacaaaata
     3961 ttctttattc atcttctttc tcacctttct tttcaaaatc aaataaagct gcttgttctt
     4021 ttatgtaaca gctttatctt tgctgcttat tgtgacaaaa aattcgaaga aagacaacta
     4081 attgtacttt tcacaaaaaa agacagaggg tcgtgacaaa attgtgtcac aacccttttt
     4141 ctttattgtg cagtttcgat ttcgcctagc gctgcttgtg caagtaggtt caagtaattc
     4201 catgggcggt caaaatgtgg ttggaagaag aaatctgaaa gtgcgagatc ttctacagtc
     4261 attttatttt gaacagctaa agacatggta tttgctgatt gtgtaatgtc gtatttggac
     4321 atgaactgag cacctacgat acgattcgtt cctttttcat agactagctc catcattact
     4381 ttttcagtag ttggcatgaa ctctggacga taattatctt caaatacagt cgcttggact
     4441 tctagatcgt tcaaagttgc gctttctttg gtcacacctg tcgaaccgat cgtccagcca
     4501 aataggtaaa gtcctgaagt tccttgagta ccacgatacg ccattttatg atctgtcaag
     4561 ttatttccca caagcaaacc ttgacgaacg gcatttgttg ccaaaggaat atagttttgc
     4621 gtgccacttg gattgtagtt gactactgca gagtctccag ctgcaaaaat gtctggatta
     4681 cttgtctgca tgtattcatt cactttgatt gccccgtttg gcagtgtatc tacttttcct
     4741 tcgatcagtt ttgtatttgg acggaagccc acacataaaa tgaccatatc tgtatcaaat
     4801 gtatctgttg gtgtagctac ttgtttcact tttccttctt catctgtgat aaattcagta
     4861 acattttcgc ctagcgcaag agtaacaccg cggtctgtca attctttttc taagatatct
     4921 gtgaatggtt tatctagata tttattcaag atgcgatcta atccatcaat caatgtcact
     4981 tgttttccag attcgacaaa agcttctact aattcaatac cgatatatcc accaccaaca
     5041 attactacac gttttgcttc tttcgcttgt gcaataattt catttgcttg attgtagttt
     5101 ttgcatagga gtacattttt cgcatcgatt ccactaatcg gtgggataat aggccatgaa
     5161 ccagttgtca tcactaattt atcataagaa accgtttctg tttctcctgt tttcagatct
     5221 tttgccgtta ctgttttatt ttctgtatcg acgtctgtta catcgtgttc cattttaact
     5281 gttgctccta atgaagataa ttcttcagga tttgagtaga acaagcctgc tgggtccttt
     5341 actactccgc cgacataaag tgcgataccg caagataaga aagaaatatt gtcatttctt
     5401 tcatatactg tcacttctgc ttctggatga ttttttaaaa tactttttac agctgcagta
     5461 cctgcatgag tacagccaat tactactact ttcatttatg gtccctccga ttaacatttt
     5521 gtccttcgac aaaactacta taacagaaat aaaacggata caacagttta atgcttgtga
     5581 attcacaaag gtttgtttgt agtttcattc cttttcaagc ataagaaccc aatatctaca
     5641 agattcttat tttaataata ttcactttta tatctaagat tgtgaataaa agacaaaaaa
     5701 ataaaaaagc tagcgttttt tttacacagc tagctttttt taggtataac acttgtagaa
     5761 actgttttta catttgcgcc ttttttgctg actctttaca atccggacag agtccataca
     5821 attccaaccg atgttcatta atctcaaaat cagtcagtct gcttgcagcc atttctatat
     5881 cttcaagccc tgggtaatga aaatcaacaa tcttaccgca gttttcacaa attacatgat
     5941 aatgacgctt tgaactgaaa tcaaatcggc tagaagcatc gccgtacttc atttcttgaa
     6001 caaatccaat cgaagtaaat aagcgaaggt tattgtaaac agtcgcaaca ctcatatttg
     6061 gaaaccgatg ttcaagtgct ttataaatct catcagccgt tggatgggaa tgatgttcaa
     6121 tcaaatattc taaaaccgcg taccgttgag gtgtgattcg aatgttctct ttttttaact
     6181 tttcgataac ctcttcgact aacttatttt ccatgtaaac accccacgtt gttattctcg
     6241 tcttatcata ctatttttac attttaaaat ccacttaaca tgaaaagaaa attcaatttt
     6301 attgtatttt aatgaaaaaa gacgtaaaac agtcgcgttc actattctac gtctctattc
     6361 aaataatttt tctcatcata aatttttgtg tgttcttttc atctttaaaa gttttttaca
     6421 taaacctatc tggagaaaaa ccaagtgcct tatatctctt ggtaaagggc tgtagataaa
     6481 taacgttcac cattgtcggg aaccacagct aataccttct tgccttttcc taattctttt
     6541 gcgactttta gcgcggcagc aattgcagcg ccagatgaga tgcctactaa aatcccttct
     6601 tttcgcccaa cttctctagc tgtttccatc gcttcgtctc cactaataga aaggacttta
     6661 tcatacacag aagtatccag tgttttagga acaaaacctg taccgattcc ttggattttg
     6721 tgaggtcccg cttctttccc ttctaaaata gcagattctg ctggttctac cccgattaat
     6781 tcgattttcg gatacacacg ttttaattct cggccagcgc cagtgatcgt tcctcctgta
     6841 ccgatacctg caacaaacgc gtccaagcca tttactccaa atgcttgatg tatctcagga
     6901 ccagtcgtct tctcatgcac aagaggattt gcttcatttt caaattgtaa tggtaaaaag
     6961 tagccgtttt cttttgccaa acgttctgct tctctgattg atccagaaat accatccgca
     7021 ccaggagtca aaatcaactc tgcgccgtac cctttcatca ataaacgtct ttcgatactc
     7081 atcgtctcag gcatcacgat gattacttta tatccttttg caactcctac catcgacaat
     7141 ccgattcctg tatttccaga agtaggttca atgatagtat cacctggttt cagtagacca
     7201 tcgtgttctg ctttttctat catacttaaa gcaatacgat cttttacact tcctcctgga
     7261 ttaaaaaact caagctttac aaaaacatct gctgaatctt ctggtacgat tttatttaat
     7321 ttgacgattg gcgttttgcc aatcaattcg gtgactgagt tatagatagc tgtcatcttc
     7381 aattcctcct tgtctttgct cttcttactt atcataatac aatctgttca tttatggcga
     7441 gtgattcgtc tttgactagc aaaaaaaaga caaagatata ccatatatgg taatttcttt
     7501 gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat aactggacat
     7561 agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt gtgcaatatc
     7621 ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat ttacatctgt
     7681 tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac ttgtggttgt
     7741 tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat taatcaatga
     7801 gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt acgctgttgc
     7861 atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat cgcccgtgga
     7921 aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat aagaagaata
     7981 ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt tggttgccat
     8041 ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat ttggcgcact
     8101 agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca tgactgaagc
     8161 atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat cgataaacgc
     8221 ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt gcggtaccgc
     8281 tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa tcgctttttt
     8341 attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct tcagctcttg
     8401 cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct taaaacaaac
     8461 ttaattaatt aaattatact tttaatt
//
LOCUS       DAXWLH010000114         8438 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXWLH010000114 DAXWLH010000000
VERSION     DAXWLH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.115"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(143..730)
                     /locus_tag="ACGE36_002027"
     CDS             complement(143..730)
                     /locus_tag="ACGE36_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384399.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            905..2227
                     /locus_tag="ACGE36_002028"
     CDS             905..2227
                     /locus_tag="ACGE36_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8384400.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2382..3158
                     /locus_tag="ACGE36_002029"
     CDS             2382..3158
                     /locus_tag="ACGE36_002029"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8384401.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3151..3720
                     /gene="rnmV"
                     /locus_tag="ACGE36_002030"
     CDS             3151..3720
                     /gene="rnmV"
                     /locus_tag="ACGE36_002030"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFF8384402.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3713..4597
                     /gene="rsmA"
                     /locus_tag="ACGE36_002031"
     CDS             3713..4597
                     /gene="rsmA"
                     /locus_tag="ACGE36_002031"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFF8384403.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5048..5677
                     /locus_tag="ACGE36_002032"
     CDS             5048..5677
                     /locus_tag="ACGE36_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384404.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5801..6910)
                     /locus_tag="ACGE36_002033"
     CDS             complement(5801..6910)
                     /locus_tag="ACGE36_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384405.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7119..7541
                     /gene="mgsA"
                     /locus_tag="ACGE36_002034"
     CDS             7119..7541
                     /gene="mgsA"
                     /locus_tag="ACGE36_002034"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFF8384406.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      7728..7826
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            7864..8400
                     /locus_tag="ACGE36_002035"
     CDS             7864..8400
                     /locus_tag="ACGE36_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8384407.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2654 a         1503 c         1746 g         2535 t
ORIGIN      
        1 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
       61 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
      121 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
      181 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
      241 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
      301 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
      361 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
      421 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
      481 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
      541 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
      601 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
      661 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
      721 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
      781 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
      841 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
      901 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
      961 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
     1021 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
     1081 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
     1141 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
     1201 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
     1261 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
     1321 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
     1381 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
     1441 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
     1501 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
     1561 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
     1621 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
     1681 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
     1741 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
     1801 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
     1861 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
     1921 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
     1981 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
     2041 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
     2101 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
     2161 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
     2221 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
     2281 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
     2341 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
     2401 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
     2461 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
     2521 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
     2581 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
     2641 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
     2701 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
     2761 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
     2821 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
     2881 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
     2941 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
     3001 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
     3061 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
     3121 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
     3181 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
     3241 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
     3301 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
     3361 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
     3421 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
     3481 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
     3541 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
     3601 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
     3661 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
     3721 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
     3781 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
     3841 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
     3901 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
     3961 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
     4021 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
     4081 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
     4141 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
     4201 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
     4261 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
     4321 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
     4381 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
     4441 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
     4501 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
     4561 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
     4621 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
     4681 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
     4741 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
     4801 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
     4861 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
     4921 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
     4981 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
     5041 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
     5101 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
     5161 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
     5221 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
     5281 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
     5341 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
     5401 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
     5461 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
     5521 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
     5581 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
     5641 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
     5701 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
     5761 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
     5821 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
     5881 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
     5941 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
     6001 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
     6061 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
     6121 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
     6181 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
     6241 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
     6301 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
     6361 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
     6421 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
     6481 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
     6541 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
     6601 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
     6661 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
     6721 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
     6781 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
     6841 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
     6901 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
     6961 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
     7021 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
     7081 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
     7141 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
     7201 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
     7261 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
     7321 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
     7381 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
     7441 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
     7501 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
     7561 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
     7621 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
     7681 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
     7741 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
     7801 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
     7861 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
     7921 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
     7981 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
     8041 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
     8101 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
     8161 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
     8221 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
     8281 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
     8341 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
     8401 aaaacaagca gtcttcccat cccagaagac tgcttgtt
//
LOCUS       DAXWLH010000115         8404 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXWLH010000115 DAXWLH010000000
VERSION     DAXWLH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.116"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(567..1592)
                     /locus_tag="ACGE36_002036"
     CDS             complement(567..1592)
                     /locus_tag="ACGE36_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8384408.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1867..3552
                     /locus_tag="ACGE36_002037"
     CDS             1867..3552
                     /locus_tag="ACGE36_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFF8384409.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTNKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3566..4849
                     /locus_tag="ACGE36_002038"
     CDS             3566..4849
                     /locus_tag="ACGE36_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8384410.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4919..5788
                     /locus_tag="ACGE36_002039"
     CDS             4919..5788
                     /locus_tag="ACGE36_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8384411.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5785..6621
                     /locus_tag="ACGE36_002040"
     CDS             5785..6621
                     /locus_tag="ACGE36_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8384412.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6647..7714
                     /locus_tag="ACGE36_002041"
     CDS             6647..7714
                     /locus_tag="ACGE36_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFF8384413.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7711..>8404
                     /locus_tag="ACGE36_002042"
     CDS             7711..>8404
                     /locus_tag="ACGE36_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8384414.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQN"
BASE COUNT         2682 a         1442 c         1756 g         2524 t
ORIGIN      
        1 tattgtcaca acctcttttt tggtttgtct aaaataattt gtctaactag aagaaaagtg
       61 ggaaaggatc ttaacaacat cggttcgtat ccaattaagg tatcggaacg atcatcttcc
      121 aagagaagtc aatcaacaaa agtaaagata gatcattttt tgaatgcaaa ataaattgat
      181 gggaagatag agcggttatt tttaatattt tgctagctat tattgaataa ttcctcgaat
      241 aaaacaaaac cagtagacat gtctgttatt ctatttatgt tttagagata agtatcccca
      301 aataacatgg attgaaataa gggtaaatgt gtttaaaaaa acgaactata aaaacttata
      361 cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa aaatagttta acacaaacat
      421 tatagttatc cacagctatc taaaacagat ggaaaataag aaaaattcgc taaaataccg
      481 ggagggggta acgagggatg agtgattgaa aaaagaacag agtaactgac ttatcctcag
      541 tgtttctgtt cttttataat tctgttctac atttcttgtt gttggatcga atctgtgaga
      601 gtaatctcgg ttgaaagaat gattcttttt tcttcagggt attggtggtg gattagttgg
      661 aataaaagtt ctgtggcttt gattcctaac tgatttgtcg gaatgtctat ccgagtcaac
      721 tgtggcgaaa atagggagga caataaggga tcactgaatc ctgcgatcga aagatcatca
      781 ggtatctgct ttccagattg ttctgccact ttgtaaatac tcaatgattt taatgcatcg
      841 gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta aatcattcat tgctgccatg
      901 ctatcttcta agcgaaagcc gccgtctata atccattcat tacggatatc taggtggtga
      961 tcaaataagc attgacggta accattgaga cgatcattgg atacataaac ttctgtcgga
     1021 gcatggagac agccgatatg acggtgtcca ttcttgatca gtgtttccac caaatcgtaa
     1081 ctatcctgga aattatccgt gtccactgag tagatgttcg tgtagtcgtt tccgactttt
     1141 ccgattacca caataggaat attgtattga tccaattcaa aaagaagtgt ttcatccatc
     1201 gtagagctta aaataataat tcctcggatc attttttctt ggatcttttt cgcgcatttt
     1261 tctagctctt cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt attttgttca
     1321 gaaacggcag aaatggattg aataacatct gaaaaaaaag gattgccata ggtcgtatga
     1381 gaagtacgat gagataatgc tagaatcgcg tcaaaaccat ttcctgacag tcctcttgca
     1441 attttgctcg gttcataatt caattctttg attgcttgaa gtacacttgc ttttgcttta
     1501 tcagagatat ttggctggtt atttaatact ctggagacag ttgattttga gacattagct
     1561 tttttcgcaa tgtcataaat agtaggtttc aattttatat gcacctcctt tttgggagcg
     1621 ttttagaata attccatagt acaatagaca ttcttttttg tctatgattt tgaaaattta
     1681 ggaatagctt gcatgatcat gagagaccat atttttttaa gaagctatta attattgata
     1741 cataagcttt cttatttgta tttttgtgta tatcaaaatt atttaaaaaa atgctattga
     1801 cagtaagcgt tttccataat atgatcaaat cgttgggaac gctcccaaaa aagaaagggg
     1861 aatccgatgt atacaaaact tttacaagcg atttatcgcg actcagattt gtctgagaaa
     1921 accgcctcta tgattgaaca aaaagtcagt gtttatcagc agaagaaagg ccaggcaggc
     1981 aaactgcatt gggacgaaga agatattttc ttgattactt atggggatca attttttgaa
     2041 gagcagcaac caactttgcg tacatttaag aaattttatc aaaagtattt ggcagataca
     2101 tttagagttg tacatttctt gccatttttc ccatatacat ctgatgatgg tttctccgtt
     2161 gtaaattatc aggcaatcaa cgaccgtttg ggcacatggg aagatataga agaaatgaga
     2221 agcgaaacac ggttaatgtt cgactttgtc tgcaatcata tgtctgccga aagtacttgg
     2281 tttcaggaat atttaaactt gaatgatacg tacaaagatt tttttattga tttgccactg
     2341 gatacagata caagcagcgt gactagacca aggaccagcc cgttacttac aacgttcatt
     2401 gacaaaaacg ggaaagaaaa acacgtatgg acgactttta gcgaagatca agtagacttg
     2461 aattttaaaa atccagaagt tttattgaaa atgatcgata ttcttttgtt ttacttagaa
     2521 aaaggcgctt catttattcg cttggacgct gtgggatttt tatggaaaga actaggaact
     2581 tcttgtatac acctgccaga aacgcataaa atcatccaat tattccgtgc aatcgctgat
     2641 gaagcagctc caggaacggt attgatcact gagacaaatg tgccacacaa agataatatc
     2701 agttattttg ggaatggcga gaatgaagca cagatggtct atcagttttc gctaccacct
     2761 ttgacccttc aagcgattcg ttcgggtaat acaaataagt tgaaagaatg gttaaaaacc
     2821 atcgatttcg catcgaaaaa aacaactttt tttaattttt tagcctccca tgacggtatc
     2881 ggattgaatc cggtacgagg tattctgccg gaaaaaacga tccttgaatt agtggaacag
     2941 ctgaagaaag agggtgcccg agtaaactac aagaaaaacc cagatggaac gaccagtcca
     3001 tatgagataa acgctacgta tctagatgcg ttaagttctc caacagatac gacggaatgg
     3061 agaagcaaac gctttttagc tgctcattca tttttggtaa cgttaccagg cgtgccggca
     3121 atttatattc aaagtgtgct gggaagtcga aatgattaca aaggcgtaga aaaaacagga
     3181 gagaatcgaa aaatcaatcg gcaaaaattt ttgttagcag atattgagaa agagttgcag
     3241 acagatccat taagaacagc agtatatggt gaattgaccc agatgctatc tattcgaaaa
     3301 aaagaaaaag ccttccatcc gaatagtttg atttacttac tggactcagc agattctttt
     3361 atcagtttta ttcgcgaagc gaaggaagag aaagtcttag ttatccataa tttttccaat
     3421 caatcagcag gttatctttt accaaaagga gattacctag acctgttttc tggacaaatt
     3481 atccattgca gtcaagaacg aaagttgata ttccagccgt atgaatatat gtggctgaaa
     3541 ataataaaat aagaggtgga aaaaaatgaa aaaaattatt gttactacct gtctgctagc
     3601 tactggattt cttcttactg cttgtggtga aagtcaaagc tctaacgaat cacaacagac
     3661 aacgattcaa ttcatgcact cttctgtcga acaggaacga ttaagtgtga tcaatgaact
     3721 ggttgctgat tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc cggtagaaga
     3781 atcagcgttc aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac cagaagtaat
     3841 ggaagtcagt caggattttg ctaaagtgat ggataaagat gaactgattg atcagacggc
     3901 agtccaaaac gttatcgagg aagctggaga agataattat tatgacggag ccaaaaacct
     3961 tgttcgttca gaagatggaa aaagctatat tgctgcacca attagcggct gggttcaagg
     4021 tatttggtat gacaaagaaa aattatcaga agctggtttt tcagaaccag aaaattggga
     4081 tgatatttta aaaattgctc aacatttcac agataaagag aataaacaat atggaattgc
     4141 tatgcctact gctgaaggaa caatgagtga gcaagcgttc tctcaatttg ctttgtcaaa
     4201 tggagcaaat gttttggatg atgaaggcga ggtaacgatc gatacggaaa agatgagaga
     4261 agcattgtcg ttttatcaaa acttatccca atacacgatg ccaggctcta atgatgtgac
     4321 agaaatcaaa gatgctttca tgaatggaac agcaccaatg gcaatctatt caacttacat
     4381 tttgccttct gtttatgaag cggggaactc agaaaatatc ggttttgcta tcccaacaca
     4441 aaaagatcaa gctgtctatg gcacagtatc tggattgacg atttcttctg gtctagacga
     4501 gaaacaaaaa gaggctgctg aagcattcac agctttctta tctcaaccga aaaacatgga
     4561 aaagtgggtc ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag tagtcgaact
     4621 ggatggttac aaagaaaatg aagtcatcaa gtcttttggc gagctgccat ctgagattgc
     4681 tagtgctttt gatgaagtac aagtctttgg actagtcggg gaaaagaatt tcacgaaaat
     4741 gggagatatc acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa ctgtcggaaa
     4801 cgaagatgtc gatcaagctt tagctgacgc acaaaaaaat acgaaataac aagaagttct
     4861 gccaatggtg ttcgaaaaaa gtagatagct attggcagat tatcaggggg aggtttcaat
     4921 gaatcgtcca aggaaacatt ctgacttaaa attagccatg ggacttttag caccaagcat
     4981 gcttttatta gctgttttag tgctgattcc aatgctttcc aatattcaga tcagtttttt
     5041 cgatatgccg ctgaatccta actcagacgc cgtttttatc ggattaagca attatcttga
     5101 aattttaagt gatagtgaat tttatcgttc tttaggttta acagttgctt acacattctt
     5161 agttgtagca ggcagtacag ctttaggatt agccgtagct attttgctta accgcaagtt
     5221 cagatttagg aaaacggtgc gttcactgat tttattaagt tatgttacgc catctatttc
     5281 tttgattttt gcttggaaat atatgttcaa taatagttat ggaatcgtca atgatctgtt
     5341 agtagatagg ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc cagttgctag
     5401 cttctttatg gtcgttttgt ttgctatctg gagatactat ccgtatgcgt ttatttcatt
     5461 tttggctatt ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa tggacggtgc
     5521 aaatgcttgg cagaagttca agatcgtgac actgccggca atcaaaccag tactagcaac
     5581 tgtgattgtt ttacgatcta tctggatgtt ctatatgttt acagatgtct atctactgac
     5641 gaataaagtc aatatcttag gcgtttattt gtatcagaca gcctttgcgt tcaatgatct
     5701 tggaaaagca gcagctatat cgattctatt gttcatcatt attgcagcgg tcgtcttgct
     5761 ctcacgaaag aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat cttattttac
     5821 gcgacgattt tgttgctatt agctgtcaat ttgtttccat ttttgatcat gctgatgaca
     5881 tcattcaagg attcacaaga agcaattgca acgacacaaa ctatttttcc gaaagaatgg
     5941 acttttgaac attatcagga tattttcaat gctgagattt ttccattcat catgtatttc
     6001 aaaaatagtt tagtgatctc cttgatagcc tccgtaactg cagtagtgat tgggatcagc
     6061 ggggcatacg ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa tgctagcttt
     6121 tacaccgttt atatgttttc cggtattttg atcgttgttc cattatttaa aatcatttcc
     6181 ggtctaggtt tgtatgatac caatacagca ctgattatca cgatggtcgt tcaaacactt
     6241 cctactgcga tattcatgct aaaaagttat tttgacacga ttccgactga tattgaagaa
     6301 gcagcaatga tcgatggtct tagccggatc cagatcatta ttcggatcat tataccattg
     6361 gctatttctg gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg gaatgattat
     6421 ttatttgctt cgattttcct gtcgacctcc gaaaaattta ctttaccaat cggtttgaac
     6481 acattattca gtacaccaga ctacatttgg ggacgaatga tggccgcttc attggtgacc
     6541 gctttaccag tcatcattat gtatgcgtta tctgaacgat ttattaaagg tgatttagtc
     6601 gaaggcggcg taaaaggata aaaagaaaag aaagaggaaa gaaaagatga aaaaattagt
     6661 tgcattagaa ccaagaatag ccggattcaa agattacgaa gaccgtgaga tcgaaaagaa
     6721 cgaagtacgt atccgagtaa ttttcgctac tccaaaacca ggaacagaaa ttgtcgactt
     6781 tagaggatta agtccattta tcgatgaaga attttcagaa gaatggcgcc tattcttgcc
     6841 aagagaagaa ggaagccaac gcggtgtggt atttggcgaa tttcaattag gaaatatgat
     6901 tgtcggtacg atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag gagagttagt
     6961 ctgcacttat ggacccattc gtgaaacgct gattacaaac ggtgttgata attataaact
     7021 acgtaaactt gatcggttgg atcaatggaa aaatgcagta tgttacgatc ctgctcaatt
     7081 cgctttaggt ggtatccggg aagcacatgt tcgagcaggt gatgctgtag caatcttcgg
     7141 tttaggcgct atcgggcaaa ttgcgattca attagctaaa aaagcaggtg ctactaccat
     7201 cgttgggatc gatcccatag cgcagcgtag agaattagca gaaaaacacg gtgccgatta
     7261 cacgctagat cctatgaaag cagatgtcgg ttttcgtcta aaggaattga cagataaacg
     7321 tggagtagat tcagttattg aaacgagtgg cagtgctgcc gcattacaag cttctttaat
     7381 agggatcggc tatggcggga tcatttctta tgttgccttt gctaaacctt ttccagctgg
     7441 gtttaatctt ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg ctcgagcatc
     7501 tagtgaacca aacccagatt acccaagatg gaaccgtaaa cgaatcgagg acacatgttg
     7561 ggaattattg atgaacggtt acttggattg tgaagaaatc attgatccag ttgtgccatt
     7621 tgcagaaagc gcagaagctt acatgcgtta tgtagaccag cacccagaag aaagtatcaa
     7681 aatgggcgtt caagtccgag gaggagaaca atgaaattcg gtacgcaaga tcaagcattt
     7741 ttcccaagag acattttaga aaaatttaga tttatcaaag actgtggatt tgaagaatac
     7801 gaaatcgacg gtaagttgtt aatagaaaat atcgacacag tcaaacaggc aatcaaagaa
     7861 acagggatcc cagtagtgac agcttgtaac ggctacgatg ggtggatcgg tgactttatc
     7921 gaagaacggc gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc actaagcgag
     7981 gttggaggca aaggaatcgt tatcccagca gcttggggaa tgttcactta tcgattaccg
     8041 ccgatggttt caccccgaag caaagaagga gactataaag cagtcagcga atcacttcgt
     8101 tatttagata aagtagccga agaaacaggt acggttattt atttggagcc tttgaaccga
     8161 taccaagacc atatgatcaa tttgttagcg gatgcgcgtc attacattga ctctaatcaa
     8221 ttgaaacatg tgcagatcat cgctgatttt tatcatatga atattgaaga agatgatatt
     8281 tcaaaagcat tgactacgca aaaagagtat gtgggtcata tccatttagc agataatcat
     8341 cgctaccaac caggatctgg cgcaattgat tttactcgcc attttcaaac gctacttcaa
     8401 aatg
//
LOCUS       DAXWLH010000116         8190 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXWLH010000116 DAXWLH010000000
VERSION     DAXWLH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.117"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(342..1241)
                     /locus_tag="ACGE36_002043"
     CDS             complement(342..1241)
                     /locus_tag="ACGE36_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8384415.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1262..1535)
                     /locus_tag="ACGE36_002044"
                     /pseudo
     CDS             complement(1262..1535)
                     /locus_tag="ACGE36_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            complement(1558..2964)
                     /gene="lacG"
                     /locus_tag="ACGE36_002045"
     CDS             complement(1558..2964)
                     /gene="lacG"
                     /locus_tag="ACGE36_002045"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8384416.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3106..4812)
                     /locus_tag="ACGE36_002046"
     CDS             complement(3106..4812)
                     /locus_tag="ACGE36_002046"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8384417.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4812..5129)
                     /locus_tag="ACGE36_002047"
     CDS             complement(4812..5129)
                     /locus_tag="ACGE36_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8384418.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            complement(5157..6137)
                     /gene="lacD"
                     /locus_tag="ACGE36_002048"
     CDS             complement(5157..6137)
                     /gene="lacD"
                     /locus_tag="ACGE36_002048"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8384419.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            complement(6140..7072)
                     /gene="lacC"
                     /locus_tag="ACGE36_002049"
     CDS             complement(6140..7072)
                     /gene="lacC"
                     /locus_tag="ACGE36_002049"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFF8384420.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            complement(7083..7598)
                     /gene="lacB"
                     /locus_tag="ACGE36_002050"
     CDS             complement(7083..7598)
                     /gene="lacB"
                     /locus_tag="ACGE36_002050"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFF8384421.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            complement(7615..8040)
                     /gene="lacA"
                     /locus_tag="ACGE36_002051"
     CDS             complement(7615..8040)
                     /gene="lacA"
                     /locus_tag="ACGE36_002051"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFF8384422.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
BASE COUNT         2515 a         1698 c         1402 g         2575 t
ORIGIN      
        1 acgctatatt taagatttca aacaactata ttaataggaa gaaaatttca tttttcctct
       61 tagttctata aaaagtgtgg ctaatttgag gcaaaatatt aaattaaatt tgccacaaat
      121 taatggctcc actattattt taaatcaaga agtagtaaat aatcttattt tttactacca
      181 gactgtcttt agatattagc ttttcgtcac ccactacagt tgacatagac ccacaaaaaa
      241 cttaaaaacc ttgcgatgta agactggcac ggagaagacc aatctatcat cacaaggtaa
      301 gagtattcaa atcttattga agactcaaat aataacaaat tttaattaag gatagtaata
      361 tcatagattt ttctagaagt ttcttgaggt gaaattttcg ttacctgtct tttatcttct
      421 aagaaattac cttcttcaag tgaagtggag agaccactcc aaggttccaa agcaatgaat
      481 ggacttttat tagttgtaga ccacagaatc aagttcggaa aatcatcaaa atctactcga
      541 agacctttac cagacttcct tgaccgaagc gttacgctac gagacttgag ctggtcaagt
      601 gtaatggcat catgggaaaa aagtgagtag tccaaatcga gaattttttg attttccaaa
      661 aaaggtgtcc gatcttgcaa atctaataat cctgtctcag gaaaactctt tggtatcgag
      721 caagactcaa cctcactaaa ttcaatataa taatcctcat agttttcacc ttctactaaa
      781 gaactattga atgctggatg ggctccaata aagtaaggca ttgttttttc catttccaag
      841 ttcgttacct gaaattcggt tctaattgat ttaccattca aggtataaac aactctcagt
      901 tcaaactgat ataggtaatt atcaagcatc tcagcatttg gtttaatact aaaggtcacg
      961 ctattatcat tgacttcttc cagaataaat tcttcttttc gaacaaaacc atgtctacga
     1021 ataagacctg taaaaaaagg tctttcttga ggtctataaa tagcccaatc atttcgtaaa
     1081 ctaccacaga taggaaatag aataggcgcc tgtccattcc aataatttgg atcagcttgc
     1141 cagagatact ctatcccatc ctcgtcttta atcgaagtca gttgaccacc aagtgtttta
     1201 aactgaacag taagatactc gttttttaat tcgatagtca caactaattc ctttcttttt
     1261 attattggaa aggcaaataa tttacctcga agtgaatagt ctcacgtatt tctacatcga
     1321 gtgattgtaa aaattcccat tcaaatgctg ataactctaa atcagaatgg caaatccact
     1381 cttttccagt taaaacatac catttaccac attttagcaa cgagggtagc ttcaagctca
     1441 ggaatcatag gacaatcttc gaaactaaca atatatacct tttcaaagcc tggtctaaat
     1501 aagtgttgta ctttttctaa agtatcggat aacaattttt tacattttca agtaatatta
     1561 ctctatcact tgagtttctg ctactttctt ataccaatga gctgatttct taggatagcg
     1621 ttcttgcgtg tcaaagtcta catagaatag tccataacgt ttttcgtatc cgtttgacca
     1681 tgagaagaca tccataagtg accagatgaa gtaaccttta acatttgcac catccgcaat
     1741 tgcgtctgat aaaacttcca agtgtttctt cacgtaatca atacgaccat catcataaac
     1801 ggtattatct acaaactcat ctttgtatcc aagaccgttt tcagtgatgt aaatcttctt
     1861 gtagtttgga taatcatttt tcaatcgcat gatttggtca tacaagcctt caggataaat
     1921 aatccagtcc caatccgttc taggcacata atctggtgcg attcggcgac caacaccttt
     1981 aatctggtac ttagaacttc ctttttcacc cttaccattg tgaatgattt cagtctcacc
     2041 atcaaaggct tgcatccaat cactcatgta gtagttgata ccaaggaaat cgttcaaatc
     2101 tttagctgct tcaagtgctt ggaagtcttc atcacgaaga tcaagttctc caccattttc
     2161 agctaggatg tggttgaccc cttccatcgt tttatctgaa tagtgtccaa ggtaggttgc
     2221 atccaagata aatttgttat ggatgatatc ttctaactca gcggcacgaa catctgctgg
     2281 attttctgga tcataagggt atttggttgg caacgcatgt actacaccaa tttcgccttt
     2341 ataaccttta tctttataca atttcacagc acgtgcatga gaaaccatca tattgtggtg
     2401 tgattggaag actttggcaa gatcatattg aatgcctgga gggaatttac caaccaagta
     2461 ttgaccatct ccaattgggc caatttcatt gaaagttgtc cagtagttta cttctgggaa
     2521 ctcttcaaaa cagaaagcgg cataatctac aaagtgttct atattttcgc ggtttaagaa
     2581 atctccatct gagtgaagaa cttctggtgt gtcaaagtgg tgcaaagtca cgaatggctc
     2641 aacatgacgt ttgtggcact cggcaaaaag attatggtag aattcaacac ctttttcatt
     2701 tacttcgcca tagccagttg ggaaaatacg agaccaggca attgaaatac gaataccatt
     2761 aacgccatat ttttcagcta attctaaatc aacaggatat ttatggtaaa aatcacttgc
     2821 aggctctgct gtgtaccaat aattgtcttc aagatactta tcccatgcaa ctgggccttt
     2881 accatccgtg tgagtcgcac cttctgcttg ataagcagct gtagcaccac caaaaataaa
     2941 atctttagga agtgttttag tcataactat aaatcctttc aaaaaaagca tttgagacga
     3001 tattctgagt tccggaaacc aaggaaatga aatgcaatga tatggttaac aatattataa
     3061 ggaactgaaa gaataatctc gtctcaaata acttttacaa atttattaat caaactgttg
     3121 ttgaacaaat gcaagagcac cttgtccatc acgtgtcaat ttgatatatt gagctccttc
     3181 agtttttgca agtttaatgc ccaatttatc cgtttcagcc ttcatatcat cgaagtttga
     3241 agcgacctgc ggagccaaga taaccaagtc aaactctggc aacatttcac ggtgagcacc
     3301 gtaactaccc gctgccgctt tgactggaac attgtattct gctgctgctt tgttcaaggc
     3361 attggcaagc aagccacttg tacctccacc tgcacaaaga acaaggacat tagtttcttt
     3421 tgtgatatta ttgtttgctg ctacatcttc tttagcaacg cctgcttttc caagaacggc
     3481 atctgcttta gcagtattga agtttgcagc tactttttct ttaagagcat cgtttgtttt
     3541 accagaacgt tcttcttcaa gaatttgttc atcgtatacc ttaacaaatg ggtaataaat
     3601 gatagtatca acaactacca agagtccggc taagataaat gatagaactt ggaagttagt
     3661 accgagtaca attcccaacg gaccaggagt aacccatgga aggttggcag agaatgagtt
     3721 catgttcaat gtgtcaacaa agaatttaaa gatccaaacg ttgacaatcg gagcgaaaat
     3781 gaacggtaca aagaaaatcg gattcaatac gattggtgca ccaaataaga ttggctcatt
     3841 aaccccaaag aatgttggaa caactgaggc acgtccgatg gcacggttac gttctgattt
     3901 acaaatccac atgaacaaga atggaacaat caatgtagct cctgttccac ccatggtaac
     3961 aataaacatt tgagtaccag aagtgataac tttatctgca tgttgtccag cttggataag
     4021 atgcaagttg gcatcgatat tggcataagt gatagcagcg attgctggtt cgacaattga
     4081 aggaccgtgg ataccaacaa accagaagaa ggcataagca ccaaagataa gtgtaattcc
     4141 aaggtaacca tctgcagctg agaagagagg agcaagaagg gtaccgattg attctgcaac
     4201 ggttacacca agagttccct taacaatgag ttcaaatcca taaaggagaa caaccgctac
     4261 agtgaatgga atcaagtctt taaatacttg agagatattt ggtggaacct cttcaggcat
     4321 acgaatagta acattatttt tcacacagac cttgtaaaca ttaactgtca caaaggctgc
     4381 aataaatgct gtcaaaagac cttttgttcc cgtgaaggct gtcaagaagc ctccttcttt
     4441 tgcaggctca gctgccatca aaaggaaccc taccatagac gctagcatcg tagataagaa
     4501 gttaatctga ttggtcgcag gtaggtcacg gttcattgag tccgtcaaag ctttagcggt
     4561 agtaccacca acaaagaatg ctaggatacc catcgaatag ctataaggag tcatcaagaa
     4621 ggtctcaatg tcttttgacc agtggaatcc ccaagcattt gggacatagg caataaggat
     4681 aaagatagat gagaaaagga tgactggcat accagcgata aatccatcac gaatagcacg
     4741 aagatagatg tttcgagaga ttttctcaaa gaacggtttc cctttctcaa taagttcaat
     4801 gagtttatgc attattttgc tcctctttta taaagttcga tgaggtgatg aatcacatct
     4861 tttaataaga cagtagtcat taagtggtcc tgaccatgca tcatggtaac gctgtatgga
     4921 agttcctccc cagaagcttc tcgagccaac atagctgtct gagaattgtg agcctctgca
     4981 atacagcttc ctgcttctac tacaagacta tctgccttag cgaaatcacc attttcagcc
     5041 gctttaagcg cttctaaaag tttagagcga gcatctccag cataagcaac aatttcaaac
     5101 cctaagagag tcatctcttc tctgttcata acaatatcct ccgttttatg tttttactat
     5161 actttatcag tccatggact agctgtttta acaagaactt tgttaagttc atcaatattt
     5221 tcgaatccag tagtacgcaa ccattcacgt gctgcttttt caccttcttt gatgtaaggt
     5281 tctacagatc ctgcccaagt agcacgtcca caaagcacac cattaaactt ggcacctgag
     5341 tcgtgagcaa attgcaatgt ttcttggaac aatttagcag atacacctgc acttaggtag
     5401 atgtatggaa ggttagtcgc ttcttcttgt gctttaaaga agtcagcagc ttcagcttta
     5461 ctgtaaacca cttcaccatc agcaaaacct tcgacatatt taacgttaac tggtacttct
     5521 actttaagaa catcaatgtt aaagcgtgga tcagagaaaa ctttcatcgc ttcaatgact
     5581 ttgcgaggtt tcacttttgc atattcaaca gaacctgcat ctgagatttc ttcatcataa
     5641 gcaaggattt ccaagaagaa aggaatatct tcagcgacac attcagaacc aacacgttca
     5701 atataagctt gtttttgttg gttaagttca tctgagctgt ctacatcata gtaaagcaag
     5761 aacttaacgg catcagcgcc ttgctctttg atacgttttg cagaccaaac atccaagcag
     5821 tcaggcaaac gttttgtact tgatgtatca taacctgttt tttcataggc aagaagaaga
     5881 cctgcttctt tatccaatgc ttttgttgct ggaagtccat attcagggtc aagcaacatt
     5941 gaagaagcgt attttgtaag ttcatcagca accaaaacct ttagttcttc catttgagct
     6001 acagttggtt ctgtatcttg atactgtgcc atcaaacgtt tcaaagcacc acgttggtca
     6061 aatgccaaag ctgagataac accgttttta tctgaaattt tttccaaaga tttgcgtttc
     6121 tgttctgtaa gtaccatttt tatacctctt ttactccaat ttgtgagttt agtgtttcgt
     6181 aattagtcat attcacatgc cctgtcattg tttcttgagc atttaacata cccaatgtca
     6241 tcgcatgttt taataagtca gcgtcacttt ttttactatt taaagctgat gcaataccag
     6301 ctaccgttga gtcccctgat ccgactggat taacaactgg gatatcagga atatctacct
     6361 tatagaaaac atcaccatgt tttgcaaagg caccgtttcg acctaatgag accactatcc
     6421 attcgatacc actaaaaagt gactctttaa gaacgtcttt gagttcttca atatcttttg
     6481 ttacttcttt tccaagcaac tgtgaaagtt cctcattatt aggtttgatg gcagtaggtt
     6541 tagccgacga ttttagaact gtttcaagag gtgcacctga acaatccaaa acgactgcta
     6601 ctccttcatc cgaagcaagt tggataagtt tttcataata atcgttggga agtcctgaag
     6661 gtaaacttcc tgaaattgtc acaacttctg attgtttaat aagattgcta tagtggtcaa
     6721 gaaagccttc tgcttcttca tgagaaattg ttggtccttg ttccaaaatt tctgtttggt
     6781 taccctcgtg taagatagca atacagtttc tagtgtttcc tgagatttta taaaaagctg
     6841 gactaacagg agattgttct aattcgcttt caataaattc accaatttta ccacctaaga
     6901 aacctgtagc agtaacttta tcaccagatt catacaagac tctagtaaca tttaagcctt
     6961 ttccaccagc tgtcttacta acatctttca ccctattcac tgtatcaatt ttcaatgttt
     7021 ctagaggata agagatgtct actgaaggat ttagtgtgac tgtcagaatc atttgttata
     7081 ccttaatcgt ggtagtcacc acggtcccat ctttcaagga attctgtaaa gaaatcttca
     7141 tctgcttgat gtgcattatg tgtttcaacg tgttcgattt ttgcaatcaa ttttttattt
     7201 tcttctgttg gtttgtactc agcttcaatg aaagcttcaa tgatgtcatt cataagaaga
     7261 ccaccagtaa tctttccacc gaaaccgata acattcgcat ttaactcttc ttttgcatat
     7321 atagctgatg tcatatcacg aaccaacgct gagcggacac ctggaacttt attgactgca
     7381 ttgttaatgc cgacacctgt accacagata catactccca agtctgcttg accacttact
     7441 actgcttcac cgactttttt accatagata gggtagtgag tacgtgcgtg gtcatatgtc
     7501 ccgaaatcaa ggacctcatg tccttttgat ttcaagaatt ctgatactgc cattttttca
     7561 tctgtgacaa tgtggtcaca tccaattgcg attctcattc cttaactcct tttcttaaca
     7621 cattttgtta agcatatcta ctcggatttg atgacgtccg ccatcatatt taccattgac
     7681 gaaaccttta gcgatgtttt tagcaagctc atcaccgacg atttcagcgc caaccgtaat
     7741 cattcgtgaa ttgttatgtc cacgtgtcat ataagctgag cgttcatctg aaacttctgc
     7801 tgcaaccata cctttaattt tagttgctac catgaatgga ccagctccat aagcatcaat
     7861 tacaatacct aagttttgct catctttatt tacttcagag gcaactgcca aggtaacatc
     7921 aacaaaatct tgtccgtcct tagtcacatc aattacctca aaaccttctt ctacaagaaa
     7981 atttttcaca acatctttta atctcgtacc tttgagatct gcaccaacaa caatagccat
     8041 tttgaccacc tcctaaattt tagaaagaaa tgctacgtag aagtagaaaa cattttgcaa
     8101 aatgttcgaa aaaacgtttt catcatttct ttgtttatat aatatcaatc attcaaacaa
     8161 aaagcaacac ttttttgttt attttttgtt
//
LOCUS       DAXWLH010000117         8150 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXWLH010000117 DAXWLH010000000
VERSION     DAXWLH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.118"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            76..1164
                     /locus_tag="ACGE36_002052"
     CDS             76..1164
                     /locus_tag="ACGE36_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8384423.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1292..2668
                     /locus_tag="ACGE36_002053"
     CDS             1292..2668
                     /locus_tag="ACGE36_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8384424.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3069..4649
                     /locus_tag="ACGE36_002054"
     CDS             3069..4649
                     /locus_tag="ACGE36_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFF8384425.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4847..5071
                     /locus_tag="ACGE36_002055"
     CDS             4847..5071
                     /locus_tag="ACGE36_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFF8384426.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5276..5596)
                     /locus_tag="ACGE36_002056"
     CDS             complement(5276..5596)
                     /locus_tag="ACGE36_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8384427.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5744..7978)
                     /locus_tag="ACGE36_002057"
     CDS             complement(5744..7978)
                     /locus_tag="ACGE36_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8384428.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
BASE COUNT         2516 a         1437 c         1622 g         2575 t
ORIGIN      
        1 taacgaatat aatatatact agtaagaaag aaaggaggaa atctattgtt tgaaaaactt
       61 agacagtcta aactaatgtt ttggtcgctt gaattattga tcatagctgc attgatcatg
      121 atttcatcag aaataaactt tattttcgaa ccgatcggga catttttttc cacgttattt
      181 gcacctgtac tgattgcagg ctttctttac tatttattga atcctattgt caagctgttg
      241 atgaaagcaa aaatcaaaag aatatgggct gtcgcaattg tgctgctcct tttagttgca
      301 gcaattattt ggattttact tagtgtcatc ccaagtctag tacagcaaat ttcctcattg
      361 gcatctaata tgcctgattt catcaaacag gtagaaagct ggttaaaaga ggtagccgaa
      421 ttgcctttgt tcaaggaagt agatatcaat aaatattttg agcaattaga catttcttac
      481 gggacaatca ttcagcaatt ccttagtgga gtatccaata gtttaggttc gatcgttgga
      541 acgatcgctt cggcaaccgt agtaatcatt acagcaccat tcatcttgtt ctatatgttg
      601 aaagacggag aaaaactagt gccaaacatc caacagtttt tcccagaaaa aagaagaaac
      661 caaattgtgg agttattggg gcaactgaat aaaacattgg ctaattacat tagcggacaa
      721 gcaatcgaat gtttattcgt tggtaccttc acatttttag gatatatggt aattggtgtg
      781 gattatgctt tcttatttgg ggtcattgcg ggattgacaa acctaattcc atatttagga
      841 ccttatctag gactagcacc tgcatttcta gtaacagtct tcaatgatcc ggtaaaagca
      901 ttactttgtt gtgtggttgt gctaatcgtc caacagttgg atgggaatat catttatcct
      961 aatgtaattg ggaaatcact gaaaatccat ccattaacga tcattatcgt tcttctagta
     1021 gctggaaata ttgccggatt gcttggtatc ttcttaggtg ttccttttta cgcaatctgc
     1081 aagacgatca tttcctatat aatcaaaatc gtcaaagaag acaagcaaaa tgaaaacaag
     1141 aagaaaattg ctacgacctc gtaggtagtt ttagtacact tgtttttatt gcaaagaaaa
     1201 ttgctttgtg ataccatttt aacgtggcta tagtgccacg tttttttcta tcgtaaacta
     1261 aaaattaatt ttataaggag agaatgcgat tatgaatgct gaccctgaga gtcagtcgct
     1321 gttagcgcag attttattat tgatcgtttt gacattgatc aatgcttttt tagcagcttc
     1381 cgaaattgct gttgtttcga tcaataagaa tcgaatcgag caaaaagctg atgaaggaga
     1441 tgtgaaatct caaaagcttt taaaaatatt gcagaatcca aataatttcc tatcgacaat
     1501 ccaagtagga atcactttgg tcaacatttt gtctggtgct tcactagcta atactttatc
     1561 ggaacggtta gctccggtat taggtggcgg cacagcggca aaaaatattg caaatatcat
     1621 tgtgctggct attttgacct atgtatcgat tgtttttgga gaattgtacc ctaaacgtat
     1681 tgctttaaat aaatcagaag aagtcgcaaa gttcacttca gggctgatac gcttgattgg
     1741 tgtagtagct aagcccttcg tctggctgct ttctgcttcc acaagtttat tggcgcgtat
     1801 cacgccgatg acttttgatg atgaagattc aaaaatgacc agagatgaaa tgcgttatat
     1861 gcttgaaaac gaaggcgtat tgaataatga agaattagaa atgctccaag gggttttttc
     1921 tcttgatacg aaagttgccc gagaagtcat ggtcccacga acggatgcat ttatgattga
     1981 tatcaacgac actgtggaag aaaatgtgaa tgaagtttta tccgaaaact attcaagaat
     2041 ccctgtttat aacgaagaca aagataaagt agtcggaatc cttcatacaa agaatttact
     2101 aaaagctgcc cataaattcg gttttgataa tttagatatc aagaaaatca tgcaggagcc
     2161 tttattcgtt cctgaaacgg tctttattga tgatctgtta tatgaaatga aaaagacgca
     2221 gaatcagatg gctattttat tagatgaata tggcggcgtc gttgggttag caacactaga
     2281 agacctttta gaagaaatcg tcggagaaat cgatgatgaa tcagatgaag ttgagaatct
     2341 ttatgaaaaa atcgatgaac acgaatatat catccaagga cgtatgctga ttgatgagtt
     2401 caatgaagct tttgacagtg atctgcatat gagtgatgta gatacgatgg ctggttattt
     2461 gattacagca ttaggcatga ttccggatga aggcgaaaag ttatcgtttg atgtcgataa
     2521 tattacgctg gtttctgaag aaatggaagg ttctcgaatc cttaaaatcc gtgtcatttt
     2581 tcatgatccg gaagaaacag aagcggaacc tgaagaagaa cgaagatact tcagaaaaga
     2641 attcgaagat gacgagccaa gaagataatt ggaaacttgg ttatgaaaga cgtgttgctt
     2701 gaataaacaa gcaacacgtc ttttctttta gtcagctgac aaaaaaatat ttgactctta
     2761 tggtttggtg gctttttcat tcgatgaatc catgctatac tgattcagtt gtagtagttg
     2821 acaaaggttg tgaaaaagct atcctgcatt aagtaataag agcagccaag caaaaacagc
     2881 ttttcgtatt tttggcttgg gtgggcttat tgctgatagg ctgtctctag aacgccgttt
     2941 actcggaaaa agagactgct acacggcttt tgtcacagcc ctatatgaaa ttgtctttct
     3001 ttaagagatc ggacaatctg tgcctagaga aaatataaat aattgaatgg aagaaatgga
     3061 gttaggttat gaataatcca gaattaaagc aacaagtgga caatcgcaga acttttgcga
     3121 tcatttccca tccggatgct gggaaaacaa cgattacaga acaattacta ttgtttggtg
     3181 gggctatccg tcaagccggg accgtaaaag gcaaaaaaac aggtaatttt gccaaatcag
     3241 actggatgga aatcgagaaa caacgtggga tttctgttac tagttctgtt atgcagtttg
     3301 attatcaagg gaaacgagta aatatcttgg atacaccagg acatgaagat ttttctgaag
     3361 atacttatcg tactttgatg gccgtggata gtgcggtcat ggtaatcgat agtgcaaaag
     3421 ggatcgaagc acagacaaag aaattattcc aagttgtaaa aaaacgtggc atcccgatat
     3481 ttacttttat caacaaacta gatcgtgatg gtcgtgaacc attggaattg ttagaagaat
     3541 tagaagagct attagatatc gaatcttatc cgatgaattg gccaatcggt atgggtaaag
     3601 ggctagaagg tctttatgat atctaccata atcgtgtaga gttttatcgt ccggaaaatt
     3661 atcaggatga acgattagta gaattggatg aagatggact gcttcccgaa aatcatcctc
     3721 taactgaaaa ttccttatat gaacaagtct tagaagaagt agaactaatc aaagaggctg
     3781 gagatgcatt taatcaagaa aaaattgctc gtggcgatca gacaccagta ttttttggtt
     3841 ctgcattgac taacttcggt gtacagactt ttttagagac tttcgttgaa ttggcaccag
     3901 ctccttatgg acacaaaaca caagatgatc agatggtaag tccttatgag gaagaatttt
     3961 caggtttcgt attcaagatc caagcaaata tgaatccagc ccatcgtgac cgaatcgctt
     4021 ttgtccgaat ctgttctgga acattcgaac gaggaatgga tgtctggttg gaacgtacaa
     4081 ataaaaaact gaaattaagc aatgtcacac aatttatggc agattcgaga gaaaacgtcg
     4141 aaaaagcagt agcaggcgat atcatcggtg tatatgatac aggcaattat cagattgggg
     4201 atacattgtt cgaaggaaaa ctaaaagttg cttatgaaga actcccttca tttactccag
     4261 agctgtttat gaaagtgact gcaaaaaatg tcatgaaaca aaaatccttc cacaaaggaa
     4321 tctatcaatt agttcaagaa ggagcgattc agctttataa aacatatcta acggaagaat
     4381 atatcattgg agctgttggt cagctacaat tcgaagtatt ccaatatcgt atgttaaatg
     4441 agtataacgc cgaagtaatc atgtctccaa tgggacacaa aatcgctcgc tggatcgatc
     4501 cagaagattt ggatgagaag atgagttcaa gtcgcaatat cttggctcgt gaccgtttcg
     4561 accaaccatt gttcttattc gagaaccaat ttgcagaacg ctggtttgcg gataaatatc
     4621 ctaatgtaaa attaaaaagt ttgatgtaaa gaagtcttga cacaagctgc cctgcatcaa
     4681 gtaataagaa tagccaaaca aaaacagtct ctcatatttt gtttagagcc gtgacggatt
     4741 ttccgtgacg gctcttttta ctatattaaa atcatagaaa tgtctaatca aaaaaatata
     4801 aaaattattt tgcgtacata tgattaaaga aaggagagtg tgacacatgg aaacagaatg
     4861 gcaaatacgt gaaagaaaaa aacagaaaaa agaagagatg ttacgattat gtcgattatt
     4921 agatttaaaa gactgggaca aaaacgagca aattgcacaa aaagtacgat caatcatcca
     4981 taaagaggaa aaagttaaaa ataaagacac gcgtagaact gaaaatgaaa agagaaaagt
     5041 acaaaagaaa agagaaaaat caaaaagcta gaaagaaaaa gaggctgaga cagaagcgtt
     5101 taactccaag aaataagctg gaattcacaa aaatttgaaa agcaattttc gtgaatttta
     5161 gcttatttcc gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac
     5221 aaaagtgttc tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca
     5281 cggctaatgt agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt
     5341 cgattacttt gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat
     5401 ttttagcatc ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt
     5461 cattatgttc aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga
     5521 ctagatgggc atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta
     5581 catttaccaa tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta
     5641 ttataatttc ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac
     5701 ggcatttctc aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt
     5761 cggctgattc tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat
     5821 ccagttttgc tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat
     5881 cttcgatttg ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc
     5941 ctaagtcaac taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg
     6001 ccagcatttg gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg
     6061 ataatgcact gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt
     6121 tgtttaattg gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt
     6181 ttgcttctac tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc
     6241 tgaccgttct tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat
     6301 gcaaaacatc tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc
     6361 tccgtacttt ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac
     6421 caatcaattt agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg
     6481 aatcttgtga accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac
     6541 cagttggtcc aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac
     6601 ggtttcggcg aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag
     6661 ctttcaagtc atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca
     6721 ctggaatacc tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg
     6781 tttcctcttc agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat
     6841 aataagctgc tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt
     6901 cttgcagttt tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag
     6961 aacctgtttc atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac
     7021 gattggataa agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt
     7081 cttcataacg tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat
     7141 ctacccgtac aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact
     7201 catttaaggt agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa
     7261 tattaccagc atccatattt ccatcaccag cagaaccagc accgacgatt tcgtgaactt
     7321 catctatgaa taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac
     7381 gttcttcaaa ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa
     7441 tgacttcttt gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac
     7501 cttcaacaac tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg
     7561 ttcggcgatt taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat
     7621 cgatatcacc ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt
     7681 ctgtattgcc ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg
     7741 gtggtgtttg ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat
     7801 cgtccaaact gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt
     7861 gttgattttt gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat
     7921 tgacagtggc atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga
     7981 atcattcacg tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga
     8041 ggtaaaagaa taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct
     8101 attgaccatt cctgaccttt gactcttatt atactaaaaa taaaaaggga
//
LOCUS       DAXWLH010000118         8057 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXWLH010000118 DAXWLH010000000
VERSION     DAXWLH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.119"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            45..1199
                     /locus_tag="ACGE36_002058"
     CDS             45..1199
                     /locus_tag="ACGE36_002058"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFF8384429.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1221..1973)
                     /locus_tag="ACGE36_002059"
     CDS             complement(1221..1973)
                     /locus_tag="ACGE36_002059"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8384430.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2337..5003)
                     /locus_tag="ACGE36_002060"
     CDS             complement(2337..5003)
                     /locus_tag="ACGE36_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8384431.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5344..6795
                     /gene="cls"
                     /locus_tag="ACGE36_002061"
     CDS             5344..6795
                     /gene="cls"
                     /locus_tag="ACGE36_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8384432.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7124..7939)
                     /gene="thiD"
                     /locus_tag="ACGE36_002062"
     CDS             complement(7124..7939)
                     /gene="thiD"
                     /locus_tag="ACGE36_002062"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8384433.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2335 a         1685 c         1447 g         2590 t
ORIGIN      
        1 ccttagtaaa gtaagttttt tatcataagt aaagaggtgt tgaaatgaaa atagggattg
       61 atcgtctttc cttttttatt cctaatttat atttagatat gacagagttg gcagaaagcc
      121 gtggggatga tcctgcaaaa taccatatag ggattggtca agaccaaatg gcagtcaatc
      181 gtgcaaatga agacatcatt acactaggag caaatgctgc cagcaaaatc gtaacagaaa
      241 aagaccgtga gctaatcgac atggtcatcg tcggaacaga atccgggatt gatcattcaa
      301 aagcgagtgc ggtaattatc caccatttac tgaaaatcca atcttttgct cgttcttttg
      361 aagtaaaaga ggcttgctac ggtggcaccg cagctttgca catggcgaaa gaatatgtca
      421 aaaatcatcc agaacgaaaa gtactagtca tagcaagtga tattgctcgt tacggcttgg
      481 caagcggtgg tgaagtgacg caaggtgtcg gtgctgttgc gatgatgatc actcaaaacc
      541 cgcgtatttt atcgattgaa gacgacagcg tatttctgac agaagatatc tatgatttct
      601 ggcgtccaga ttatagcgaa tttcctgttg ttgatggtcc tttatctaat tctacgtata
      661 tcgaatcatt ccaaaaagtt tggaatcgac ataaagaatt gtcgggtcga ggactcgaag
      721 attatcaggc gattgctttc catattccgt atactaagat gggaaaaaag gcattgcaaa
      781 gcgtattaga ccaaacagac gaagaaaatc aggaacgtct tatggctcgc tatgaagaaa
      841 gcatccgtta cagccgacga atcggtaatc tttacactgg ttcattatac ctggggctaa
      901 cttctctact ggaaaattcg aaatcactac agccaggaga tcgcatcggt ctattcagct
      961 acggttctgg tgcagtaagt gaatttttta caggttatct ggaagaaaac tatcaagaat
     1021 atctcttcgc acaatctcat caagagatgc tagattctcg aacacggatc actgttgacg
     1081 aatatgaaac gattttcagt gagacgcttc ctgaacacgg agaatgtgca gaatatactt
     1141 cagatgtacc tttttcgatt acaaagatcg aaaatgatat tcgttactac aaaatataat
     1201 aaaatcataa aaagaaccct tcacgagtcg tgaaaggttc tttttttatc tgcggaccaa
     1261 aggagcagtc tataggtcag cttagtcgta ctaaggattt ctcgtttttc actgaatagt
     1321 ttgcgaatgt acctggtcac aacaggatct tttgcgtacg cgtcatagta tagcttcatc
     1381 aattcttcag taattgattc ctgtgtaaaa taagtaaaat cttttttctt cactttgaat
     1441 ccttcatcaa ttaagtatgt ttcaaattga tccattacaa ctggcgatgt attaggatct
     1501 tcttctatca ttcctagttg cttttcgata ggataaaaaa catcatcgat tgattccact
     1561 actctgatta tcacgcacca tttgcttgac tttttcatca atttttctaa ttgaataaga
     1621 tgtaccaatg ctggatttgc tgctgtaaaa accatttcat atgtctcatt tgattggata
     1681 aattcttcta acgactgttt gataaaggaa acatttggca actcattttt ttctgcttct
     1741 ttttttgcat tttctagcat tcctgaagaa aaatcgatca aatcatagcg attaatttct
     1801 tttgctaagg aaagaaatct gccagatcct ccagcaacgt ctgctgctgt ttcagcaggc
     1861 aggactttct ccgttttcag ataatttttt acatcttcag caatagttac ttgcgattct
     1921 tgctggattt cataatattc ttcagcaaat tcatcccatt ctttttcatt catttgattt
     1981 cctctaattt tttgttatat gttttcactt ttcggaataa atcattctcc tcttttatct
     2041 attctatctc ttttgatgaa taataaaatg attggctttc cgtctatcat aaataaaaaa
     2101 agggactgtg accaaagtcg tgtcaaagtc cctctttccg aataaacagt gtccaatcag
     2161 atgaccctca gtattagctc aaaatgctga ttttacaact tcttccttaa acggtgttct
     2221 cctgccaact cttcggtcac aagtcacaat attcaacaaa catgagaagc tgtttgttga
     2281 aattgcacct tgtgactcag aattaaacgg caggctcaca acctcgttga ttcgttttat
     2341 ttatttcctt gtactcgtac aatgatcaat ttagtgattt ccatcaaaac aattgtcaca
     2401 agtgcgattc ccatacatgt aaagacttct gtcaatccaa atgctgatgg gatagagaag
     2461 acttctttca gtcctgggat cattgttaag cagtaaagcg cgctacatac aactacagct
     2521 agtaagacac ttctgttaga gaagaaccct gcttggatag ctgtttgtgt gtttgaacgt
     2581 gctgggaatg tttgcaatgt ccgacaccaa atcaatgttg aaaatgacat ggctacaccg
     2641 aattcaggtg aatgctgcat gccaagccat tgagcaaaga taacagcaat cccaatcagc
     2701 acaccacgat acgttacaga aatcaatgtt ttgtccgtaa agattcctga atttggatct
     2761 cttggcgggc ggttcatcgt agcaggttca gctttttctg tacctaatgc aattgctggc
     2821 agtgaatcat tgactaaatt gatgaacaac agttgtaatg ctgtaaatgg attggaccag
     2881 tcagcaacta aggcaaagat gatcgcaatg atcgcaccta aatttcctgc aaatagataa
     2941 gaaatagctt tcttgatatt atcaaacaca ttacgtccta cttctactgc gctgacaata
     3001 gaagcaaagt tatcatccgt caataccatc gctgctgcat cttttgccac atctgtaccc
     3061 gttcccatag caattccgat gtctgcttgt ttcaatgctg gtgcgtcatt tacaccatca
     3121 cctgtcatag ctgagatatg acctttattt tgccaagcac gaacgatacg aattttattc
     3181 tcaggtgaaa cacgtgcata aacagtgact ttttctaaaa cttcgtttag ttgatcttct
     3241 gtcaattcat ctagttccgt cccggtcaaa gccaggtttc cttcttcaaa aatcccgatt
     3301 tgttttgcga tagcaacagc tgttgttttg tggtcaccag taatcatgat tgttttgatt
     3361 cctgcacttc ttgcttctgt taccgcctga gtcacttcag cacgtggagg atcgatcata
     3421 gcaagcaaac caacaaggat caaatcgtct tcatcttcaa atgataaatc aaaattatca
     3481 agtggacggt aagcaaaagc tagtacacgt aatgcgcgtg ttgaaaaagc ttcattttgc
     3541 tcttggaatt ttttcatcaa ttcatccgtc atcggaacga cttcaccatt tagcaatact
     3601 tttttactgc gttggaagac gatatctggt ccgcctttaa caaacaagta atttttgtta
     3661 tcaattgtgt gtccagtaga cattaatttt cgatctgagt caaaaggaag ttctgcttgt
     3721 cttgggtagt tgtttcggat ttcctgataa ggctggttta atttttcact aaagtcgata
     3781 aacgctactt ccgtagggtc gcccaattta gtaccgtctt cactgatcga tgcatcattc
     3841 gctaaaacac tgatttcgat caagcgacgt tcgtcagctg tccattttga agggtcattt
     3901 gtgaaatccc cagtatcgcc attacctaaa aagtagtcaa caactgtcat tttgttttgt
     3961 gtcaatgtac cagtcttatc tgtacagatg atacttgtcg aaccaagtgt ttcaactgct
     4021 ggaagcttcc gaataatggc atgacgtttt gccattttat tcgttcccaa agataacacg
     4081 attgttacga tagactgtaa tgcttctgga atagcagcta cggctacggc tacggcaaac
     4141 atcactgcgc tgacgatatc tgcagtcata tctcctgtac cttctccaag ataaatacga
     4201 aacagttgga tacctaaaat caataaagac aatgccaaaa tagccaaact tagtttttta
     4261 ctgaatttat ccagacctcg ttgtaacgga gtgacttgct cagttgttga ttccaaaagg
     4321 ccagcaactt gtccgatctc cgtattattc ccagttgaag tcactaagaa tttaccgcgt
     4381 ccgtaagtta cgatcgttcc actgaatacc atatttgtac gatctccgat tggtaccgta
     4441 cttgtgatat ccgttacttc tttttctgca gaagtcgact ctcctgtcag cataccttca
     4501 tctactttta atgatcccgc atctagtaaa cgtccatcag caggtacata atcgcctgcg
     4561 tctaacaaca cgatatctcc taccacaagt tcgtttgccg gaatcgtttt ttccttacca
     4621 tcacgaatca ctcgagcatc gggcgctgaa aggtttttca acgcttctaa agaaccttct
     4681 gctttttttg tttggacaac actgattata gaattcaaca tcaatactgc aaaaataact
     4741 aagaattcga tgtagtcacc catgaccatc tgaatcagtg cgacaatcag caatacgata
     4801 accattgcat ctttgaaagt ttctaagaac aatttccaag ttggcttctt cggcttttct
     4861 tttagctggt tcaagccatc tctttctaat ttttccttcg cttgcgtggt gctcaatcca
     4921 tcggttgagc tctgcgtttg ctgcatcaac gagtcgatcg tttctttata gaccgcttgt
     4981 tgtgcttgtt ccttgttttc caaatttcat cccccatttt ttgatcttgg ccctagtcct
     5041 gtctgaaaaa aaagagacct aagacaaata cgcatttgtc ttaggtctca ccatttcata
     5101 caataccaga aaaccatttg gttaacttac tgttgatatt atcgcaacaa acgttgccgg
     5161 ttactcccct aaggagatat tcaactgtac taatatccta ccttattaac ctacaaaaag
     5221 caactacttt agcccgattt acctacattt tcatgaaagg agtgaaaagt atattcacct
     5281 tggtaggctt ctttcttcta tcatgctata ctaatgttat tgtatgtaag gaggaacatt
     5341 tttgtggtat ctagtattat aaccgccctt tatctattaa atgcacttat tgctttggtg
     5401 gctattttga tcaaaccccg agatgtagca gccatttggg catggctctt agtattgttt
     5461 gcccttcctg gggtgggctt tgtattatat ttatttttcg gacgtggatt aacggataag
     5521 aaaaaattct atctccgaca aagtgacttg aaagaattag aaaactttca gtcttttaga
     5581 ggagatacca ttgaacatta cgatcctgac atgggcgata aagacaaaca gcaatttgtt
     5641 gacttcttct cttcattaaa tcgtatgccg ctgacaagaa tgaattctgt cactcttctc
     5701 acagacggac aagagaaatt ggattcactg cttcaagatc taaaaaaagc caaacattcg
     5761 atccatatcg aatattacgc atttgtgaca gataatatcg gccagcaagt cttacatgtt
     5821 ttagaagaaa aagccgcaga aggcgtggaa gttcgaatat tatatgatgc atttggctct
     5881 catggcacaa aagcaaaaga tttcaatcgt ctaatcaaaa atggtggaca tgtccataca
     5941 tttgttacct cacaaagggc attacttcgt ttccgattga attaccatga tcaccgaaaa
     6001 atcgttgtga tcgatggaaa gattagttat accggtggtt tcaatattgc caatcaatat
     6061 gtaaatacaa caaaaaaatt cggctattgg cgcgatacgc atatacggat tttcggtgcc
     6121 gcttcttctt tgctccagct tcgcttctta acagactgga acgtctcggt acctgaagaa
     6181 aaaaaggtcg gctatcattt gaattatttc tttaaaaaag cagatcgaga tgaatctaag
     6241 cttgctgata catccatcca gcttgtttca agcggaccga ataacgaaag ggaacaaatc
     6301 aagctttcat ttatcaaatt gattacttct gctaaaaaac gtgtttggat acagacacct
     6361 taccttgttc ctgatgaaag tgtcattgct gctttaaaaa tcgcaactgc ctctggtgta
     6421 gatgtgaaaa tcatgattcc caacaaaccg gaccatcctt ttatttatcg agcaacacaa
     6481 tattatgctc ggcagctgat caaggaaaat gtacaaatcc ttgtctatga gaacggcttc
     6541 ctccatgcaa aaacattgat aatggatgat gaaatctgca tggtaggttc agcaaatcaa
     6601 gatattcgta gctaccgatt gaattttgaa acaagtgctg tcatttacga tcctgagttt
     6661 ttagaagaac ttgctactca gttcaaagaa gatgagacac attgttcatc catgacaact
     6721 gaaacagtca aggaaatgtc taactggcta ttattcaagc aacaaatttc tcgattattt
     6781 tctccaatcc tataattttt ttattcaagt agagaaaaat atactaagat taaagggact
     6841 gtgacaaaaa tcttgtcaca gtcccttctt ccgaacaaac ggtgttctcc tgccaactcc
     6901 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaac ttgttccttg
     6961 tgactcggag ctggacggca ggctcacgac ctctttctta aacggtgttc aaaagcttgc
     7021 atctgcggta ataagtccaa ccaagttaaa aacataagaa gctgttttta cttggctgtt
     7081 cttattactt gatgcagggc aactttttca caacttcttt cttttactct aattcaccag
     7141 ctgcccaatg attgatgggg ccatatttgt gccctacatt tatttcttca cgaattgcac
     7201 ggtttacata gtcttttgcc aatcggatcg cttgttcaac tgtttttcct ttcgctaact
     7261 cagctgcaat acatgcagat agcgaatcgc cagttccgtt gatccggtcg gtaggataaa
     7321 atggtgcatt taaccaaaat gattttcctg attcgaacaa tacgtagtca ttgactgttt
     7381 cttgctgcgc atttttagca tgatgtcctt tgatcataac gttttttgca cccatatctt
     7441 gcagttcttt tgctgcttgc acaaaatctt cttcagctgc taatgtccgt ccagtcagct
     7501 tttctgcttc ataaaaatta ggtgtcagta cttcggccaa agggacaagc ttttctttca
     7561 agctttgcaa agcagattct tctagtaaaa gatttccatg tttcgttacg attacgggat
     7621 ccacgactaa tggtccaaaa tcatattttt ggtaattttt tacaactgtc tcaatcaact
     7681 ttgaatctgc tagcatacct gttttagctg cacgtatttg aaagtccttc gcaatcagtt
     7741 caaattcttt gtcgataaaa tctgttggca atgtcacact tgctccgatt ccgtaagaat
     7801 tacctgcaac acatgcggtc atgacagaaa caccgtaaac atttcttgta aagaatgtat
     7861 gcatatctgc ttgcatccct gcgcttccat cgctatctga tcctgcgatc gtcattgctt
     7921 gtacatactg atcttccatc aaatcgattc ctccattttt ataaaaagat ttttcataat
     7981 actgttactg tttctatcat atgtttttca aagtatccct cagtaaatat accactacct
     8041 tttcttggta atcaaat
//
LOCUS       DAXWLH010000119         8028 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXWLH010000119 DAXWLH010000000
VERSION     DAXWLH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.120"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(116..874)
                     /locus_tag="ACGE36_002063"
     CDS             complement(116..874)
                     /locus_tag="ACGE36_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8384434.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            1042..1977
                     /gene="pfkB"
                     /locus_tag="ACGE36_002064"
     CDS             1042..1977
                     /gene="pfkB"
                     /locus_tag="ACGE36_002064"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8384435.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            1974..3419
                     /locus_tag="ACGE36_002065"
     CDS             1974..3419
                     /locus_tag="ACGE36_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8384436.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            3447..3902
                     /locus_tag="ACGE36_002066"
     CDS             3447..3902
                     /locus_tag="ACGE36_002066"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFF8384437.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            3921..4895
                     /gene="lacD"
                     /locus_tag="ACGE36_002067"
     CDS             3921..4895
                     /gene="lacD"
                     /locus_tag="ACGE36_002067"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8384438.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(5006..5567)
                     /locus_tag="ACGE36_002068"
                     /pseudo
     CDS             complement(5006..5567)
                     /locus_tag="ACGE36_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            5711..6325
                     /locus_tag="ACGE36_002069"
     CDS             5711..6325
                     /locus_tag="ACGE36_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8384439.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            6566..6949
                     /locus_tag="ACGE36_002070"
     CDS             6566..6949
                     /locus_tag="ACGE36_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8384440.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            7312..7821
                     /locus_tag="ACGE36_002071"
     CDS             7312..7821
                     /locus_tag="ACGE36_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFF8384441.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
BASE COUNT         2625 a         1280 c         1688 g         2435 t
ORIGIN      
        1 atagaaggag caatgataga aagcacgaga atacaaatag cgattaaata tttttctaga
       61 caaccttagt taattcacac agatatattt taggtacagt cttacagata tagagttagt
      121 gctgtgtgaa tcctgatgtt cgcagtgcat aggagtcaag taagattcta gccagtatag
      181 aaagtggaag tctggagcgt acctcataat ctgggaagaa ggagccttct gatttaaagc
      241 caacaaaagg aatctcgata tgttccatca aacttgaatg acgattagca gtaagcaaaa
      301 ttgtcccgat ctcagcttta taacagtttt cagctgcggt agtcaattcc tttgtttcac
      361 cgtttagtga aacaaagatc acgatgtcat ctttggataa ttttctacta atgtttttta
      421 tgatattggg atcagtatgc atctcacaat atttaccggt aagttgaaat ttgaccatca
      481 tttcctgagc aatcaattct gaaaaacctc gggcaaaaat catgattctt ctagcagcct
      541 tgattcgttg aatagcatct tcaatattcc cgctttctac catattgatg gtgcggatta
      601 cctcttgctc atttttaaga atagcagtct ttattccttc atcaacttta tcaagagcgg
      661 caaaattgat atttgtattt tctttttctt ttaacgaatg tttaaaagac gtaaacccct
      721 catatccttt tttcttcata gtacgaacga cggttgctgt tgaaacgttg gcatattcac
      781 ttagttttac aatggaatag gaggggattt cgagtatgtg actttgaata aaatcccaga
      841 gatatttctc cgattcactt aattttgtat ccatattttg cctccgatta gttaggataa
      901 ttctattgta aagcgggagt ttcctttact tcaagtttat ttaatatatt ttgtaaaagt
      961 tttcaatgaa tttagtcatt tatgaaaaca tttacattcg atcttgatac ataatgaata
     1021 tgtaagaaag gaggagcgta aatgtcagtt tatacatgta ctttaaactt ggcaatcgac
     1081 ctttttatcg aaacagatga aatgaaacca tcccaagtca atcgaacaaa cgatgatgat
     1141 atacaagcta atggaaaagg tgtgaatgtt tcattgatca tgaagcaatt aggtatcgat
     1201 agtacagctt taggatttaa agcaggtttt accgggaatt atattgagga ttatttgcat
     1261 caaaaagata ttagcactga ttttattagc gtaccgggaa tgactagaat caacgttttt
     1321 acacaagtta ataaaaccaa tgaagaattc aaattggtta ataaaggacc tgagattcct
     1381 caggaacaaa tcaaaacttt tctaaatcaa atacaaaagc tgcaagctga cgattatttg
     1441 tgtgtttctg gcagtttgcc gcgaggggtc tcacccgaaa ttcttattga tatcagtcgt
     1501 atttgttttg aaaataaggt ccatctgatt attgattcaa gctatccgga aataattgat
     1561 tgtctgccgt atcagccgtt cttattaaaa ccaaacgagg aggaattgca atcttggtat
     1621 aatgtcgagc ttagtgatcg tgaaggatat attcattatg ctgaacgctt gattgccgat
     1681 ggagcgaaaa atgttctcct ttctctagga ggagagggag gaatattttt aaataaggat
     1741 atcgtttatt ctgggaattc tcctaaaggt aaagtagtca atacagcgtg tgctggtgac
     1801 actttgttgg gaacatttct tgcaggatat ttaaaaggtg aacaggtaga tattaatttg
     1861 aagaaaagtt tagcagctgg aagttcaacc gcttttcgta aaggattaac agatttttcg
     1921 gatgttgcag agttggaaaa acaaatcgaa atcaaagagg gagcgagtaa aaaatgacaa
     1981 cttacaaaat tgttgccgca acaggatgtc caacaggaat tgcccacact tatatggctc
     2041 aagaagcatt ggagcaggca gctaagcgca aaggggtatc tatcaaagtt gaaacccatg
     2101 gtcaagtagg gatagagaat gaattaacac cagaagaaat aaaaaacgct gacgctgtaa
     2161 tcattgcagc tgacaaagat gttcaagcag aacgtttcgc aggtaagcgt gtaatcgatg
     2221 tgcctgtcag caaggggatt aaagatgctg accaattgat cgaagatgcg ttggctggta
     2281 aaggttcaat cactgcagaa aacaaaacag ttgacgctgc tggtgagaaa gcggagatgg
     2341 gggttgcagc aggcattggt cacagtattt ataaaaatct catgaacggt gtttctcata
     2401 tgctgccgtt tgtagtggca ggtggggtaa ttattgcaat ttcgtttgcc atctggggtg
     2461 tgtactcttt tgatcccaat gatcctactt acaatccgac tgccgctatg ttaaagggtg
     2521 ttggtgatgc ggcaatggga atgatggttc ctattttagc agcttatatc gctgaaggaa
     2581 tcgctaaacg tcctggtttg gttgttgggt ttgttggcgg actgttagcc aattctggcg
     2641 gagccggttt cttaggcgga atttttgcag gtttcttagc aggctacttt atcttactat
     2701 tgcaaaaggt catgaaaggc ttgcctaaac aattggatgg tctgaaggcg atcttcttat
     2761 atccggtgat cggtgtcttg tttgttggtg tagtcatgtc tttattagca aatccaatgg
     2821 aagcagtcaa ccaaggaatg atgaatttct tggcaggatt ccaaaattct aatccattaa
     2881 ttttagggat tatcgttggc tgtatgtcag catttgatat gggaggtcca gtcaataaag
     2941 cggcttatgt tacagggact gcgttgttgg cacaaggtaa ccatacattt atggcagggg
     3001 tttcggcagc ttgtatcgcg cccccattga ttactggatt tgccgcactg ttttttggaa
     3061 aatatttcga cacgaatgat cgcaacgctg gtttagtcaa ctttatcttg ggctcaacgc
     3121 acattaccga aggagcaatt ccatttgcgg ctaaagatcc gctgcgtgtt ctgccaacta
     3181 tgatgctagg ttcatcaatt gctgctgtac tgacatatat gtttggtgtc caagttcccg
     3241 cacctcatgg aggttttcta gtattacctg ttgttactgg taaggttgca tgggtagggt
     3301 cgattttact tggttcaatc gttggcggag taattttagg gttgatccaa aaacgtagtg
     3361 tagaaaaacg tgaagcagaa gcagctgctg ttgaaatgaa agaagcaacc aacaactaaa
     3421 ggttattcta gaaagaagga tttcaaatga ctttgatcaa aacaaataaa gtttttatga
     3481 ataaaaattt tacgacgcaa gatgaggtaa tgaacttcct tgcagatcag tcaattgtct
     3541 tgagcattag tgaagaccga gatgcagttt acgaaaaatt gatggaaaga gaagaagaaa
     3601 gcacgactgg tatgatggat ggttttgcca tccctcatgc caaagatgct agtatcaagg
     3661 atgccgatat tgtgattgtt cggttaaacg gtaaggtcga ttggaaaagt ttagatggag
     3721 aaggaacgga ttttgtaatc gctctgttta ttccagattc agaggctgga acaacccatc
     3781 taaaattttt atctcaggta gccagacttt tgatgcacaa agatgtgaca gaaggtctga
     3841 aacaagccca gtctgctgaa gaaatagctg aacttttaaa ccaaaaatta gaagaaaact
     3901 aaaaaggaag tagtgaaatc atgcctttaa cagaaaaaaa gaaaacttat attcgtcaac
     3961 taagtaatga aagtggcatc atcagtgcgt tagcaatcga tcaacgtggt gccttgaaaa
     4021 aaatgatcgg taaatttcaa aataaatcag caaccgatga acaaatcatc aatttcaaaa
     4081 aaatcatttc ttctgaattg actccatatg cttcagcaat cttattagat ccagaatatg
     4141 ggttgccggc agccgaagtt cgcgataaaa atgctggtct gttgttggcc tatgaaaaaa
     4201 ctggttacga tgcttcaact ccaggacgtt taccggatat tttatctatt tggtctgtaa
     4261 aacgtctaaa ggagcaaggg gccgatgctt gtaagttctt gttatactac gatgtagatg
     4321 aatctgatga catcaatgac caaaagaagg cgtttatgga gcggattggt tctgaatgtg
     4381 ttgctgaaga tttgccattt ttcttagaat tggtttctta tgatgccaac gatcctgata
     4441 cttcttcgaa ggaatatgcc ttgaaaaaac cgcataaagt aaatgaaatg atgaaggaat
     4501 tttctaagcc tcgctatgga gttgacgttt tgaaagtgga agttcctgtt aatatgaact
     4561 atgtggatgg ttacactaaa aatgaagttg cttataccaa agaagaagct gctgcttatt
     4621 tcaaagaaca atccgagact accaacctgc catttatttt cttgagcgca ggagtaactg
     4681 ctgaattgtt ccaagaaacc ttgaaatttg caaaagaggc tggctctaca ttcaatgggg
     4741 tactctgtgg gcgcgcaact tgggcgaatg gtgtagaacc attcgttact aaaggtgaat
     4801 atgctgccaa agaatggatg cagacacaag gacgcaagaa catcgaagag ctgaataatg
     4861 ttttgaaaga aacagcaaca gctattaaat tgtaagaaag ctttaactat aaccttaagt
     4921 ttgtaaccta atgattttta ataatcaatt tgagctttta aataaagaat tggaagcgtc
     4981 ccgacgcttc ctttttcgtt tgtttttaag gcatgatagg ttctatgttg tcgttcaata
     5041 agcgcaagaa ggtcagcatt tttttcttag agtatgcaaa attgcttttc tacccaatga
     5101 tttaaaacgg agaggcaccc ttggatatgg tagatggtga agtaacgcat ttcttctgtg
     5161 atcacggttt ttttgtcttc ttgtagccag atttttaaat aattttcttc gatcagatta
     5221 tttaatctct tttgaaattg gatatctccg tggttgatga cgaataactt gtatacgaac
     5281 gtattcgtat agacatagtc gatcaattga gcgtatagat cctcgtagct atgtgtcgta
     5341 tcgtaggaaa tgatttcatc caagtcttgg aaaaagctat tttccagttt ttcataaaga
     5401 tcatagatat ccgtataatg tgtataaaat gtcgaacggt gaagatcagc agtttcggtc
     5461 agttcacgga tggtgatgtt ttgaattttt ttatctacta ataattttga taatgcttct
     5521 gctaacgctt tttctgtttt tttgacgcgg cgatcctctt ttttcatttt gcagcctcct
     5581 aatcaacata atcaaggaat tgtgtcggtt atccgacaat ttcaccgcta tctgaacata
     5641 gataaccgac accttgtcta ttataatata agtatagcat atcaatattt aaagaatagg
     5701 agaataagtg atggaattag cctttaagca actggaaaag aaagactaca aaaaagccat
     5761 tcaattcgcg attaaaggta tgcactttga ttggtatacc aagaacaaac tccttttgaa
     5821 tctttatgga agatactttt ggtatctaga attgaatcgc gcgacacaaa tcattgcttt
     5881 atatgcggat gatgagttgg caggtgtttt attagcagac atcaaaggaa aatcaaaaaa
     5941 gcatcattca tttagtcaaa agttatatgt taaactattt gatttcttac aaaatgcatt
     6001 tgtgaaagac agcaaaggcg ggtatgacga caccaatgac gaattgcttt cagactattt
     6061 aaaaaaacat tccccagatg gagaaattgt ctttttagct gccaaccctg atttaaagat
     6121 caaggggatc ggtagtaaat tattaaaaga atttgaacgc cgagagcaag gaaaagaagt
     6181 atttctgttc acggatagcg gctgtacgta tcaattttat gaacgacgcg gtttcgaacg
     6241 gaaaaaagaa aaagataacg tcatgaagat ccttaataaa gaagtagatc tccgctgtat
     6301 gttatatagt aagatcattc agtaatgttt tttactatca gaaagcagaa gtaccacgaa
     6361 ggtcaatgga aaaagcgagt aattgatcca tttagatgaa gataggtgta gtctcaaaga
     6421 tattagctgg caaagatatc cccagttaaa aatagatttc tgtatccaac aataaaaata
     6481 tttttgtatt tattagtata tatactttac aaaaatttgt atatatacta atttttttgt
     6541 aggatcaatg aaatgagggg aaaatatgcc aaaatataaa gaaatagcct tggctctaaa
     6601 agagaggatc gttgagggaa aatatcagga aggttctaaa ttacctgagc aagaaagtct
     6661 ggctagagag tttaaaacaa gccgtgttac tatccgcaaa gctatacaac tccttattga
     6721 tgaaggtttg ttatatacga gacgtggatc tggtactttt atacgaagca atatcagaca
     6781 ggacaatgag aatgtcactc aagtaaataa tgtatttggc acttcctcac aagaaaatac
     6841 aaatgtgaca agtaaaataa tccgttttga tgtccggttt ccgacagatt atgaaaggcg
     6901 gctttctgca acctacttcc gatccccttc ctgcaaatca tgggtataaa aaataggcat
     6961 attaagcggg ctaggaggag aaatattcac atatcatttt ttcattccgc aagctgcgtc
     7021 aagttatttg gaatttgtag aacagatgag agaggtagaa gcagcactac aaactacttt
     7081 tcaataagta ggaattatag aaattttact caaaaaatct tcattaaaaa atctgactaa
     7141 ataagttgag aaaacagaat atacttcaat atccagtgat ttataaatat cattcactca
     7201 tgatacccag ctccgtatca taagtgatgc ggtttttatc attgatccta aaaatcttag
     7261 caagacaaaa agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac
     7321 aatgataggt tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa
     7381 gcttttgagc taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa
     7441 gtacaggata agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac
     7501 caagtttttg acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat
     7561 aagccgatag tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg
     7621 ctaaaaaaat taaaaatcgc cttgtctatg accactgatg atattttaga agtttttgct
     7681 gaggcagaac tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa
     7741 cgaaatttta agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt
     7801 tataatcgga gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat
     7861 gagagctagt ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt
     7921 atgaatgatg aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga
     7981 tgtaggctca attttgagct tcggggaaag atgtctttaa tacaacaa
//
LOCUS       DAXWLH010000120         7963 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXWLH010000120 DAXWLH010000000
VERSION     DAXWLH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.121"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            229..1620
                     /locus_tag="ACGE36_002072"
     CDS             229..1620
                     /locus_tag="ACGE36_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384442.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1693..2340)
                     /locus_tag="ACGE36_002073"
     CDS             complement(1693..2340)
                     /locus_tag="ACGE36_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8384443.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2348..3034)
                     /locus_tag="ACGE36_002074"
     CDS             complement(2348..3034)
                     /locus_tag="ACGE36_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384444.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(3040..4113)
                     /locus_tag="ACGE36_002075"
     CDS             complement(3040..4113)
                     /locus_tag="ACGE36_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384445.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4335..5009
                     /locus_tag="ACGE36_002076"
     CDS             4335..5009
                     /locus_tag="ACGE36_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8384446.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            5014..6354
                     /locus_tag="ACGE36_002077"
     CDS             5014..6354
                     /locus_tag="ACGE36_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8384447.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6503..7489)
                     /locus_tag="ACGE36_002078"
     CDS             complement(6503..7489)
                     /locus_tag="ACGE36_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFF8384448.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2511 a         1446 c         1373 g         2633 t
ORIGIN      
        1 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
       61 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
      121 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
      181 gagggagaaa aacatatgat caggttgttg gaattaaact atagaaagat ggaaaaaatc
      241 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
      301 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
      361 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
      421 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
      481 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
      541 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
      601 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
      661 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
      721 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
      781 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
      841 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
      901 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
      961 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
     1021 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
     1081 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
     1141 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
     1201 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
     1261 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
     1321 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
     1381 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
     1441 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
     1501 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
     1561 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
     1621 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
     1681 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
     1741 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
     1801 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
     1861 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
     1921 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
     1981 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
     2041 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
     2101 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
     2161 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
     2221 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
     2281 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
     2341 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
     2401 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
     2461 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
     2521 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
     2581 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
     2641 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
     2701 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
     2761 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
     2821 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
     2881 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
     2941 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
     3001 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
     3061 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
     3121 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
     3181 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
     3241 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
     3301 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
     3361 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
     3421 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
     3481 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
     3541 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
     3601 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
     3661 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
     3721 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
     3781 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
     3841 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
     3901 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
     3961 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
     4021 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
     4081 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
     4141 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
     4201 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
     4261 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
     4321 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
     4381 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
     4441 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
     4501 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
     4561 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
     4621 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
     4681 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
     4741 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
     4801 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
     4861 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
     4921 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
     4981 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
     5041 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
     5101 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
     5161 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
     5221 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
     5281 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
     5341 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
     5401 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
     5461 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
     5521 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
     5581 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
     5641 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
     5701 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
     5761 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
     5821 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
     5881 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
     5941 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
     6001 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
     6061 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
     6121 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
     6181 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
     6241 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
     6301 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
     6361 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
     6421 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
     6481 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
     6541 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
     6601 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
     6661 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
     6721 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
     6781 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
     6841 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
     6901 ttggtttgcg atcgatatct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
     6961 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
     7021 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
     7081 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
     7141 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
     7201 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
     7261 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
     7321 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
     7381 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
     7441 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
     7501 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
     7561 ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg aaatttgaaa
     7621 acatttatgt taatgaaagt catattctaa taaaatgtca tttgtttcta tatgaatttt
     7681 tatgaaaaat ttcatataat ttccactcgt tgtgatggtt taatttatcc aatccatcaa
     7741 taagcaaaca acttatataa aaaaggatta attctcaata taattgctcc taccatagaa
     7801 taaatcttca tgtaaagtaa tgaagtacca actatttcta atacttcttt aagaattatc
     7861 ccaacaatat cgttagacaa accattattg aataaatgga gagccaatcg attctgaaaa
     7921 aatgttttct aatttttcgg aaataaaaga aagagcctcc tgc
//
LOCUS       DAXWLH010000121         7903 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXWLH010000121 DAXWLH010000000
VERSION     DAXWLH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.122"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(119..499)
                     /gene="yajC"
                     /locus_tag="ACGE36_002079"
     CDS             complement(119..499)
                     /gene="yajC"
                     /locus_tag="ACGE36_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFF8384449.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(596..1741)
                     /gene="tgt"
                     /locus_tag="ACGE36_002080"
     CDS             complement(596..1741)
                     /gene="tgt"
                     /locus_tag="ACGE36_002080"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFF8384450.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1959..2657
                     /locus_tag="ACGE36_002081"
     CDS             1959..2657
                     /locus_tag="ACGE36_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8384451.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2825..5464)
                     /locus_tag="ACGE36_002082"
     CDS             complement(2825..5464)
                     /locus_tag="ACGE36_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8384452.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6083..7114)
                     /gene="queA"
                     /locus_tag="ACGE36_002083"
     CDS             complement(6083..7114)
                     /gene="queA"
                     /locus_tag="ACGE36_002083"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFF8384453.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7307..>7903
                     /locus_tag="ACGE36_002084"
     CDS             7307..>7903
                     /locus_tag="ACGE36_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384454.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         2234 a         1716 c         1456 g         2497 t
ORIGIN      
        1 acacccgatt attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa
       61 taaaaaatga ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt
      121 atttttcttc cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa
      181 ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag
      241 tccctggttt gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt
      301 ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga
      361 tcgttacgac tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt
      421 gtttcttttg cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa
      481 gcattggtaa tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt
      541 agcagaaaaa tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga
      601 agctttttgc gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa
      661 gcaaattatc atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata
      721 aattgtggta agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga
      781 tataggcacg tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga
      841 aatcatgggc atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc
      901 catttcgagc gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac
      961 catcgatcaa tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga
     1021 tcaacggtgt agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata
     1081 acccgccgat tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac
     1141 gaagatcttc aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag
     1201 catgagcttt caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt
     1261 agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca
     1321 atttattttg gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt
     1381 gatttttgaa atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt
     1441 gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc
     1501 cagcttcttc tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa
     1561 taacgccagc acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg
     1621 ttcctactgg catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta
     1681 aacgcgcgcc agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca
     1741 ttcatttccc tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca
     1801 agtgcttttt gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc
     1861 ctttttttat gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat
     1921 ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa
     1981 aacgccaagc aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa
     2041 tccctacatt gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg
     2101 ccaccaccat atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta
     2161 gttcaggagg cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta
     2221 tttcttggac ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag
     2281 atgcatttcg tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga
     2341 tctcgatctt tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct
     2401 attcttattc tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac
     2461 ctaaagtact ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac
     2521 ttttctggtt agatctttca tttatcggct ggcatatctt agcattagca actgctggta
     2581 ttggttatct atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat
     2641 tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc
     2701 gtttatcgag aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac
     2761 attcatgtca cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag
     2821 ttaatcattc ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg
     2881 cgattggtac gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta
     2941 ggccgaatag ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca
     3001 gtaacgccat ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc
     3061 tgctgcgtac attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag
     3121 tttgaccgac atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta
     3181 aataataacc aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca
     3241 ataaacctcg agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt
     3301 gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa
     3361 acagtagttg cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa
     3421 taaagatctc cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat
     3481 aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa
     3541 tcatatctgc ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt
     3601 tacttgcttt caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac
     3661 ccgtacgttg ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga
     3721 cagagagagc tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc
     3781 ctgaaagtgc ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag
     3841 tcaccacatg atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga
     3901 ttgctccttt actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta
     3961 agtctttttc cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac
     4021 caacagcaat cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt
     4081 ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga
     4141 tgtatttttc gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa
     4201 gctcgtttac tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa
     4261 cagcccttac gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt
     4321 gctcgaccgt cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg
     4381 tcattgaatc ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca
     4441 tttcattttg tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg
     4501 tttcaactgc tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt
     4561 atgcaagtgt caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg
     4621 aaatcgaagt catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata
     4681 ctaaagccgc agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga
     4741 cctgtaaagg tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt
     4801 ctgtagccat gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc
     4861 ctttaaatac catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg
     4921 catctttttc aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg
     4981 atgcttcgat caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat
     5041 cacccactac cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg
     5101 ccgtttgttt attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga
     5161 caatcgccaa taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat
     5221 ccccatgttc tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga
     5281 tcgttgtaaa atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct
     5341 catcgaattt attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc
     5401 cagtatctgg atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt
     5461 ccatcctatt cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat
     5521 ccttttttta tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat
     5581 ttaaagtgct tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg
     5641 aggataccat caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg
     5701 ttagaagtac aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg
     5761 cgtatggatg tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt
     5821 gaataacgta aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg
     5881 taacgaaaac aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg
     5941 atggaaagtt acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc
     6001 ttatcgagag ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca
     6061 aaactagcgg cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac
     6121 gatagcgctc ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg
     6181 cactaactaa catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta
     6241 ccactttgaa ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga
     6301 tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag
     6361 ccacgatttt cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca
     6421 aacggtagaa ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga
     6481 atgtacctaa ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct
     6541 catcaagtaa ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt
     6601 ctttcgcata gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag
     6661 gcaatggcat ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt
     6721 caatgatccg tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac
     6781 caaaagagat tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg
     6841 tatctccttg tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt
     6901 ctccatataa acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct
     6961 gtaactcttc aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca
     7021 aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg
     7081 gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc
     7141 ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt
     7201 ttttgttttc ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac
     7261 tagtaataga tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca
     7321 aaatgtgtca aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt
     7381 tgaaaagggt gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat
     7441 gcggatgatc aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc
     7501 catccaagaa attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat
     7561 tggattgatg cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa
     7621 agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc
     7681 ccgtgagatg ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg
     7741 agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc
     7801 gcttgatcca atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt
     7861 gggaaaaacg atcgtatttg taacccatga catggatgaa gcg
//
LOCUS       DAXWLH010000122         7881 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXWLH010000122 DAXWLH010000000
VERSION     DAXWLH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.123"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(60..488)
                     /locus_tag="ACGE36_002085"
     CDS             complement(60..488)
                     /locus_tag="ACGE36_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8384455.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            580..1440
                     /locus_tag="ACGE36_002086"
     CDS             580..1440
                     /locus_tag="ACGE36_002086"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFF8384456.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(1532..1882)
                     /locus_tag="ACGE36_002087"
     CDS             complement(1532..1882)
                     /locus_tag="ACGE36_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFF8384457.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(1897..3624)
                     /locus_tag="ACGE36_002088"
     CDS             complement(1897..3624)
                     /locus_tag="ACGE36_002088"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8384458.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(3838..4764)
                     /gene="trxB"
                     /locus_tag="ACGE36_002089"
     CDS             complement(3838..4764)
                     /gene="trxB"
                     /locus_tag="ACGE36_002089"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFF8384459.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(4909..6342)
                     /locus_tag="ACGE36_002090"
     CDS             complement(4909..6342)
                     /locus_tag="ACGE36_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8384460.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(6342..7745)
                     /locus_tag="ACGE36_002091"
     CDS             complement(6342..7745)
                     /locus_tag="ACGE36_002091"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8384461.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2164 a         1769 c         1346 g         2602 t
ORIGIN      
        1 gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa tgttatttta tttaaatcct
       61 tattgacaat tttcacatct tccgaaaatc tcgaatctat gcccttcgat cgtgcatcca
      121 cctagctgtt cttcgaaata attcatcgga cacatatgga tttctttcgt tgttccgcac
      181 actgtacaga taaaatgatg gtgatggccg attttcccct gacagcaatg aaaacgaaac
      241 ttcatttctc catttaaatc ggtttcttcc agtaaatcga tctctgcaaa atcatggaga
      301 ttccggtaaa tcgtatcata acttacccct ttgaattcct gattcatata gtcaaaaact
      361 tctttagcag atacgtagcg gttacgcttc actaaataag ttaacaatgc ttctcttttt
      421 ttagtatatt taaaaccgtt atttttcaat gtttctaatg attgttcgat caatgtctgt
      481 tttttcatga cttattcctc caaatcaaaa tgattacgaa gtacgcattc tagagtatgg
      541 tataataaac ataaaaaaaa tgcaagtgag gttttatcca tgtcttccga ttctacagaa
      601 atcgttactt tttttgtcat ctccgactct gctggagaaa ctgcgacaaa actagcgcaa
      661 gctactatgg ctcagtatcc taccgttgag ttcaacctat tccgcagaac gttcgtgaca
      721 gatgaaaaaa cattaaggca ggcattgcaa gatgcattag aagaaaaagc acttgtcctg
      781 catacattaa tcaatcaaga actgatcgag ataacacgtt ctttttgtga agaacatcaa
      841 ttatttagtt ttgatgtgat gacgccaccg atatcagaaa ttgaacgttt aactggtatc
      901 aagccaattc ggcaacctgg tgccctccat ttattgaatg agaactattt caaacggatc
      961 aaagcaatgg aatttgctgt aaaatacgat gatgggaaag atcctcgtgg ttttttagaa
     1021 gcagatgtcg ttttgctagg cgtatcccga acttccaaaa cgcctttgag cttgttttta
     1081 gctaacaaaa atttaaaagt tgccaattta cctttgatcc cacaagcaca tattcccaaa
     1141 cagctttggg agatcgatcc taaaaaaatc gtgggactaa cgaataaccc agatatttta
     1201 aataatatcc gtaaagaacg gatgcgctct tacggattaa accctgatac tgcttattcc
     1261 gatatcgaaa aaattagagc tgaacttgat tttgcgaatg atctttatga aaaactagga
     1321 tgtgtagtga tcaatgtggc atctttatct attgaagaaa cggcttcgct tattttgaat
     1381 gctttagacc tagaagatca tagttattat ggaactgaaa caacagaaaa aagcgattaa
     1441 tcatttttgt ctttttctta aaaaacactg gagtgtgaca acgaagaaat ttcgttgtca
     1501 cactccagtg tttatctatt tgcacctatc gttattgatg ttccacatgt ttgagtgtct
     1561 ggttcaaaat atccagtaca tgagaatcat caagactata caaaatcgct tttccctcgc
     1621 gatgagactt gacgacatga ttttctctta ataaacgtag ttgatgcgat acagcagact
     1681 gctccatttc cactgcttgg gtgattgctg tcacattttg ttccccgtct ttcagatata
     1741 gcagaatttt aagacgagtg gggtcactca acattttgaa taattgactg actttctcta
     1801 cttttgattt ttgcgtgtct ggcaatccat tcacctccaa cttgaaaaaa gactgacttc
     1861 taaagtttac cactttttcc atcagtctca agttttttat tcttctgtca tctttttgat
     1921 aatcgcttca taagaggcta ctttttcggc tgcgtcttct gtagagcttc cttttacacc
     1981 aatataaaac ttgattttcg gctcagttcc tgaagggcgg atagcgatcc agccattttc
     2041 ttccaatgta tacttcaata cgtcggatgg cggtgttgtt agtttttcta cgtttccttc
     2101 acggtctgtt ctcgtcaatt ctttaaagtc ttctgtttga ataacttttg ctccggcaaa
     2161 ttctgtcggt gcttctttac ggaatttttc catcaaagca gagattttcg cagaaccttc
     2221 ttgtccgctc attgtcacag aaatcgtttt ttctgcgaaa tagccatagg tttcaaaaat
     2281 cgtttgcaag ccgtcataca acgttttgcc ttgtttttta tagtaagctg ctacttctgc
     2341 taataaaagc aatgcttgga tcgcgtcttt atcgcgaaca aaaggtttaa ctaaataacc
     2401 ataactttct tcgaatccaa acataaaagt atgtgaatgg tcttcttcaa attgttggat
     2461 tttttcagca atgaatttaa accctgtcaa cacattgaac atagtagcac cgtagtgttc
     2521 cgcaattgct gtaggcaatt cgctggaaac gattgatttc aataccgctg cgttttctgg
     2581 taaagtgcct cttgattgat gtgcttccaa aatatattga gcaaacaatg ctccgatttg
     2641 gttcccagtc aatacttcat aagaaccatc tggcaaacga accgctgcgc ctaaacggtc
     2701 tgcatcagga tctgttgcaa tcagcaagtc agctccctct ttttctccaa gtcgtatagc
     2761 atattcaaaa gctgaatgtt cttctggatt tggcgatttg accgtagtga aatctggatc
     2821 tgccacagct tgttcgggaa ctagtacgaa ttgttcgaat ccagcttgtt tcaacgcacg
     2881 ttcgcccaac attttccctg ttccatgaag tggcgtgtac acaagtttca aatctttccc
     2941 cattgtttca accaattcac gatcaatcgt cactgctttg atttcttcta gataagcagc
     3001 gtccacttct tctccgatga tcgtgatcaa cccgctatgt ttagcttctt cgtcagataa
     3061 tacttcgact tttagcggat tgctgacttc acggacatat ttcgtcaatg catctgcgtc
     3121 tgcaggaggc atttgtccgc catcttctcc gtagacttta tatccattgt aagccgctgg
     3181 gttgtgagat gcggttacca tgattcccgc aaacgtcttc aaatgacgaa ctgcaaaaga
     3241 aagctctggt gttggacgca gactttcgaa tacataagac gggatattgt gatgtgccag
     3301 tgttttggct gcttccattg caaattctgg tgactgatgg cgtgagtcat aagcgatagc
     3361 gacacctcgg cgttttgttt cttcaccttg tgtatccatg aaacgtgcta gtccttctgt
     3421 tgcttgtctt accgtataga tgttcattcg attgatccca ggtcctaaga ttccccgcat
     3481 gcctgcagta ccgaattcca acggtgcata aaaagcatct tcaagttttt caggatcttc
     3541 acccagatca tttaattgtt tttttaagtt ttcttctaaa ttttcttcat gtacccattg
     3601 ttcatacact tgttcccaag acatgaaaat aacacctctt tctgaaatat ggttcttaat
     3661 aagtatagca ttcacttatt agatatgaaa gtatgaggac caaaaaacgc ttaaatcaag
     3721 aaaggattct tttatcaagc atgattgcaa gtattcacat aaatcggatt cagtggattt
     3781 cacgcgaaaa aaaagagtaa aagaacaatt gaaaccgttc tttcactctt tttaaactta
     3841 tgctgttgtt tcttctaaag actccaagta tttatacact tgttgtccag caataccgcc
     3901 gtcaccaaca gctgtagtga tttgtcgcaa atctttttca cgaacatctc cgatggcaaa
     3961 aacgcctggc atattcgttt tcatttcgtt gtctgttgga atccagccag cttcgttcgt
     4021 caaacctgct tctctaaaag gtgcagttaa tggatccagt ccaacataga tgaatactcc
     4081 gtctgctgct aaatcaaatt cattgccggt tttgacatta cgtgctttca cacctgtcac
     4141 taccatttcg tttccaacga tttcgtcaac gactgtatcc cacataaagg aaattttttc
     4201 atttgcaaat gcacggtctt ggatgatctt ttgtgcacgt aattcatctc tacggtggac
     4261 gatcactact tcagaagcaa attgtgtcaa atagatggct tcttctactg ctgagtcccc
     4321 acccccgatg actactagac gtttgttacg gaaaaacgca ccatcacaga ctgcacagta
     4381 agaaacgcct cgtccagcaa attcttcctc gccaggtacg cctaatttac ggtggataca
     4441 gcctgttgcg ataatgactg tttttccttc gtaactttta tcttcacaga tgacttcttt
     4501 atggctgccg tgatccttga tatcttgaac gattccataa gcattttctg tcccaaatct
     4561 tgagacactt tcatacatct tataagctag atcggggccc atgatcgaat cgaatccagg
     4621 ataattttcg atttctgccg tattgttcat ttggccgcca ggggcacctt gttcgatcat
     4681 caatacggat aagtttgaac gtgaagcata caaagcagca gtcatcccag caggacctgc
     4741 gccaataacg attacatcat acatatgtag attctccttt acttaatcaa gtaagcatac
     4801 tttacagcca atgcgaaaaa aagtctattg ttctgcttgc gtttctcgaa tagctttatt
     4861 atactcgaaa aatccgctga aaaacaatag acttttataa atcattgatc aattgttcga
     4921 aagattttct ccatttgaat caattacttt tttataccag ttgaatgatt ttttctttga
     4981 acgggctaat gtgccatttc cttggttatc acgatcgaca taaatgaatc cgtacctttt
     5041 cttcatctca ccggtaccag ctgatactaa atcgatacat ccccaagtcg tatagcctaa
     5101 tagatcgact ccgtctagtt ctactgcatc cttcatcgcc tggatatgcg ctgccaggta
     5161 atcgattcgg taatcatctt ccacataacc attttcatct ggcgtatcga ctgcacctaa
     5221 gccgttttct acaataaata atggtttttg ataacgatca taaatatcat tcatcgttat
     5281 tctcaagcct aatggatcaa tttgccaccc ccattcactt gcttgcagat atggattttt
     5341 gacagaagca aagatatttc ctgcagtctg ttccaagagt tcgttgtcgg tagtcgaaac
     5401 tcgtgaagaa taataagaaa aagagataaa atcaacagta tgttctttca gcaatgcggc
     5461 atcccctggt tccatttcga tctgaatgcc atttcgctcc atttctttca atgcataagc
     5521 gggatattct cctcgtgact ggacatcaat gaagaaataa ttttctcgat ctgattttct
     5581 agaagcccaa acatcttcag gattgcaagt gtatggataa tatgctcctg ctgccagcat
     5641 acagcctact ttgttctccg gatctacttc atgagcaatc ttcgttgcga tggcacttgc
     5701 aaccaactca tgatgtgccg cttgatattt gatttgttct tcattttctc cttcttcaaa
     5761 atacagccct gcgcccataa atggtgcatg aaggatcata ttgatctcat tgaatgtcag
     5821 ccaatatttg acaagtcctt tatagcgatt gaagatgact cgacacagat tttcataaaa
     5881 accaacgagt tctctggaac gccacgcacc atattctttg atcaaatgca ttggacaatc
     5941 aaaatgcgta atcgtcacaa gtggctcaat tccgtgcttt tggcattctt taaagacatc
     6001 ctcataaaat ttcaagccag cttcattcgg ctctttctca tcccccattg gaaaaatacg
     6061 actccaggcg atagacattc gataagtctt gaaccccatt tctgcaaaca aagcaatatc
     6121 ttctttataa cgatgataca tatcgattgc aatttttgct ggataaaaat gatcctcatc
     6181 aaattcaaac atttttttct ttccggtgat aacaggaaaa cggtcttttc cgattggcac
     6241 aacatctacg tttgctagtc cgcgtccacc ttcatcataa gcgccctcac attgattggc
     6301 tgctgtagca ccgccccata aaaaatcttt tctgaacccc attatacttc ctccattata
     6361 tgtgtattac ctgataattc tcttgccagt tcatcttgaa cttcgattcc ttcttctttt
     6421 aatcggctga ttgtttcctt gaattgcggt aaatactgct ggtgtgcaat aaacaactca
     6481 tccatgatcc gttttgctgt ctttcctgat cgtataagtg gattgatcgt aaacgcctgt
     6541 aaagctgtcc cataatcacc agtcacagct gcttcgatcg tcagcaattc catattcttc
     6601 attacttgca accagcctct ttcagcagca ggaagagaac caaacgctac atttctcgct
     6661 ccttgtgcgc caacataggc agtgacttct acaacacaat ctgccgacag gtcaggaaca
     6721 gctccatcat tctttgtaga tacaacgatc tgtgtttctt tatttccata gattgaagca
     6781 atcgtttcac aggcagcgtc tgaataataa gcgccacctc gtttttgcaa ttgttcaggt
     6841 ttatggtcta attctggatc tttgtataat tcaaatagct ctgcttctgt ttctttcact
     6901 tgctgcgctc ttgtaccaat cgtttgatat tcttctaaag catgttccag catttcttct
     6961 tcacgatagt aatagcggtg gtagccacaa gggatcatgt tcatttgttt taattgttct
     7021 ttgaaaaacg ggatatcaaa gatatttttc ggtaatccat tatcttcatc gtataaacga
     7081 tcaatcaatt ctgccgtgcg gtcatttcct ttattatctg ctactttgtg ccaatggaaa
     7141 tgatttagac cggcaaactt gtagatcagc tcttcttttt tcttgccaag cattgctggt
     7201 tctgtcatca tcgccatcac tggcacattg cataatccaa tcactttatc ccatttgcca
     7261 tatctgataa ctgcttctgt caccatccca cttgggttcg cgaaattgat cagccaagca
     7321 tttggacata aacgcttcat gtcctcaacg attgccaaaa cgactggaac ggtacgaaaa
     7381 gctttgaata tcccgccagc accatttgtt tcttgcccaa tcatcccata ataactaggg
     7441 atccgctcgt ctttgattcg tgcatctaat tgaccgacac gaaactgagt cgtaacaaaa
     7501 tccgcatctt tcaatgcttc ttcccgatcc agagttgcat agatcttgac ttcatatgga
     7561 gaagcatccc acatacgctg agacatctga ctaacgatct caagcttttc ttttccttct
     7621 tctacatcta cgagccagat ttctcgtatc ggtaattcct catatctttt gataaatcct
     7681 tccatcaact caggggtgta actgctgcct ccaccaatcg tagcgatttt gattcctttt
     7741 gccatgttct cactccttta cttttttaca ctttcattat cttttttgta aacaaaacaa
     7801 acaatagaaa tccgtttctt tccagtaaca tttacaaaag ttactgtttt ctagtggatt
     7861 tttgtaaata tcttgtcaaa a
//
LOCUS       DAXWLH010000123         7842 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXWLH010000123 DAXWLH010000000
VERSION     DAXWLH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.124"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(78..150)
                     /locus_tag="ACGE36_002092"
     tRNA            complement(78..150)
                     /locus_tag="ACGE36_002092"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACGE36_002093"
     CDS             531..1100
                     /locus_tag="ACGE36_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFF8384462.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACGE36_002094"
     CDS             1087..1947
                     /locus_tag="ACGE36_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8384463.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE36_002095"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE36_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFF8384464.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACGE36_002096"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACGE36_002096"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFF8384465.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE36_002097"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE36_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFF8384466.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACGE36_002098"
     CDS             5995..6864
                     /locus_tag="ACGE36_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFF8384467.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACGE36_002099"
     CDS             6919..7728
                     /locus_tag="ACGE36_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8384468.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         2675 a         1326 c         1705 g         2136 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg ac
//
LOCUS       DAXWLH010000124         7748 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXWLH010000124 DAXWLH010000000
VERSION     DAXWLH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.125"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..547)
                     /locus_tag="ACGE36_002100"
     CDS             complement(<1..547)
                     /locus_tag="ACGE36_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFF8384469.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEE"
     gene            complement(549..1313)
                     /locus_tag="ACGE36_002101"
     CDS             complement(549..1313)
                     /locus_tag="ACGE36_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFF8384470.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1321..1998)
                     /locus_tag="ACGE36_002102"
     CDS             complement(1321..1998)
                     /locus_tag="ACGE36_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFF8384471.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2035..3420)
                     /locus_tag="ACGE36_002103"
     CDS             complement(2035..3420)
                     /locus_tag="ACGE36_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFF8384472.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3630..3714)
                     /gene="ffs"
                     /locus_tag="ACGE36_002104"
     ncRNA           complement(3630..3714)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGE36_002104"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3737..4252)
                     /gene="tadA"
                     /locus_tag="ACGE36_002105"
     CDS             complement(3737..4252)
                     /gene="tadA"
                     /locus_tag="ACGE36_002105"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFF8384473.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4359..5216
                     /locus_tag="ACGE36_002106"
     CDS             4359..5216
                     /locus_tag="ACGE36_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384474.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5297..5632)
                     /locus_tag="ACGE36_002107"
     CDS             complement(5297..5632)
                     /locus_tag="ACGE36_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384475.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5699..7069)
                     /locus_tag="ACGE36_002108"
     CDS             complement(5699..7069)
                     /locus_tag="ACGE36_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFF8384476.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7147..7683)
                     /locus_tag="ACGE36_002109"
     CDS             complement(7147..7683)
                     /locus_tag="ACGE36_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8384477.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2192 a         1657 c         1257 g         2642 t
ORIGIN      
        1 attcttctgg aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt
       61 tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa
      121 aaccgaaaac aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc
      181 gatcattctt ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca
      241 cagcataatc cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag
      301 aaactgggaa atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta
      361 ggtgatcaaa atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca
      421 aatgtccgat atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac
      481 tgatcagtgt cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat
      541 ttttcatatt agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc
      601 aacccaggta catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta
      661 ttgcgtatcc cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct
      721 ttttgcagcc ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg
      781 tagatcggcg gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga
      841 agaaggccgc gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct
      901 ttttgaatag caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct
      961 accactacat agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca
     1021 atcaatcctg cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa
     1081 tcgattgttg ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca
     1141 tctgctagac gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag
     1201 aaaggtaatt ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt
     1261 gtcccacgat aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca
     1321 ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg
     1381 ttcttgtttt tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac
     1441 cggttttttg cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg
     1501 gttttttttc ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat
     1561 gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat
     1621 tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg
     1681 ttcataaccc caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc
     1741 tccaagcttt tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc
     1801 acctatcaaa aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc
     1861 tttaggctcc tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc
     1921 ctgttcagct gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc
     1981 attttgtttt gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt
     2041 gattgggtag tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa
     2101 aaattcattt tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc
     2161 tacggtagta aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg
     2221 atggttaata gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg
     2281 aaatcccatt cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa
     2341 aactaagcgg atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg
     2401 caacgcagtg tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag
     2461 accactgttt aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac
     2521 ttggataaac gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac
     2581 ttctttttct tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc
     2641 ttctgtaaaa gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt
     2701 tttctcttca tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc
     2761 tgctatttgg acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc
     2821 taaaataaca tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag
     2881 atgactcatc aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa
     2941 aatatcataa ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc
     3001 agtaaatgca attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt
     3061 cgtccatgcc ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa
     3121 ctcagcatga tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt
     3181 tcctatcgtc gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt
     3241 cgttgcttct gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt
     3301 tgtcgttttt tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct
     3361 tggccaattt tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat
     3421 gtaccctctc gcctctcttt atctttatca taccataggc agctttttct tccattgaaa
     3481 caagctctat ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat
     3541 aaatcagtct ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa
     3601 aacagacccg aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta
     3661 gtgctgcttc cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg
     3721 caaagaatag tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc
     3781 gctggcggag ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc
     3841 caccttctac atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac
     3901 cggctgttcc gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa
     3961 tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca
     4021 agcgccagct tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag
     4081 tagcatcttg cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata
     4141 caaccactgc tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg
     4201 caaggcgcat ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt
     4261 ccctgccttt gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt
     4321 ttttttctat ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg
     4381 ctctttaccc acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt
     4441 ttgcaattga tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca
     4501 gccttttgaa agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact
     4561 atctttttaa agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg
     4621 tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc
     4681 gctattctgc tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa
     4741 accgtgatta tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt
     4801 ttgatacgac aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc
     4861 ctttgtttgc tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca
     4921 gccgtacttt ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag
     4981 atagtaagtt gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca
     5041 ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc
     5101 atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa
     5161 aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat
     5221 agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt
     5281 caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat
     5341 cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga
     5401 tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt
     5461 ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag
     5521 tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac
     5581 tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca
     5641 gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc
     5701 agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa
     5761 atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa
     5821 atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat
     5881 tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga
     5941 aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact
     6001 caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa
     6061 aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt
     6121 tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa
     6181 caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag
     6241 ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa
     6301 tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa
     6361 acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca
     6421 aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg
     6481 catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat
     6541 caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt
     6601 tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct
     6661 cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac
     6721 cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa
     6781 cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg aaagactttc
     6841 cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat
     6901 ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt
     6961 tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac
     7021 gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc
     7081 gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt
     7141 tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt
     7201 tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa
     7261 acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg
     7321 acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt
     7381 tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat
     7441 gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc
     7501 ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt
     7561 agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc
     7621 cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta ctctccccat
     7681 cattcttacc tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa
     7741 atattatt
//
LOCUS       DAXWLH010000125         7694 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXWLH010000125 DAXWLH010000000
VERSION     DAXWLH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.126"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            176..1057
                     /locus_tag="ACGE36_002110"
     CDS             176..1057
                     /locus_tag="ACGE36_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8384478.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1070..2680
                     /locus_tag="ACGE36_002111"
     CDS             1070..2680
                     /locus_tag="ACGE36_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384479.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2757..3416)
                     /locus_tag="ACGE36_002112"
     CDS             complement(2757..3416)
                     /locus_tag="ACGE36_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8384480.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3699..4364)
                     /locus_tag="ACGE36_002113"
     CDS             complement(3699..4364)
                     /locus_tag="ACGE36_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFF8384481.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4511..5116)
                     /gene="dhaL"
                     /locus_tag="ACGE36_002114"
     CDS             complement(4511..5116)
                     /gene="dhaL"
                     /locus_tag="ACGE36_002114"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFF8384482.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5144..6154)
                     /gene="dhaK"
                     /locus_tag="ACGE36_002115"
     CDS             complement(5144..6154)
                     /gene="dhaK"
                     /locus_tag="ACGE36_002115"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFF8384483.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6129..6524)
                     /gene="dhaM"
                     /locus_tag="ACGE36_002116"
     CDS             complement(6129..6524)
                     /gene="dhaM"
                     /locus_tag="ACGE36_002116"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFF8384484.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6678..6998
                     /locus_tag="ACGE36_002117"
     CDS             6678..6998
                     /locus_tag="ACGE36_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFF8384485.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7110..>7694)
                     /locus_tag="ACGE36_002118"
     CDS             complement(7110..>7694)
                     /locus_tag="ACGE36_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8384486.1"
                     /translation="NKRKELEEALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEK
                     KLAEAKAKAEAVQTDLDGKIQQAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGK
                     ISEAQTVANDFEAKKADLAASFQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQ
                     TLPKTNEKTASVSYSLAGAGVLVAAAYGIIRKKK"
BASE COUNT         2013 a         1599 c         1480 g         2602 t
ORIGIN      
        1 ttgagaaagt atcaagctag caattccttg tttatgaatt cattgacaga aatttataaa
       61 aagaatatta tgtactaaaa taataaaaaa agttttataa aaaggtataa aaccttttta
      121 ttaaactatt taatcttgtt tagtatatat ttttagaaaa aggagtgggg tttttatggc
      181 agaaatcgtc aaagtcacac atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga
      241 tgtctctttc accgtacatt caggtgaagt cgttggattt atcggaccaa atggttctgg
      301 aaaatccacg acgattcgga cacttttagg gattataaaa aaagacggcg gaacggcaac
      361 gatttttggg caagatgtat gggaagatag tttatctatc catgaacgtc tttcttatgt
      421 tccaggcgat atctctttgt ggggaaattt gactggagga gcgatcatcg atctcttcat
      481 gaagcttcat ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct
      541 tgatcctaag aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag tgggcttgat
      601 tgctgctttg gctgttgatt cggatctcta tttatttgat gagcctactt ctggtttaga
      661 tcctttgatg gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc
      721 catcctactt tcttctcata ttctcagtga agtcgagcga ctagcggata aagtcgtgat
      781 catacgcgaa ggaacactcg atgaagaagg aacactcgat gaattgcgtc atttgactcg
      841 ttcgaccatc actatagaaa cagaagcaga gaatagtcac ttagcacaat tgaacggtgt
      901 ccatgacttt gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg aagctttcga
      961 tgtgatttta gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac
     1021 tcttgaggat ttattcatga gacattatga aggatgagga ggaagcgaaa tgactgataa
     1081 attttcacgc tggtccaccc ttttttctca aaaccttagc cgtgactgga aaaagatcct
     1141 gatatggcta gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt
     1201 gacttccgga caaggattag ctggattata cgaaaccttg caaaacccgg caatgattgc
     1261 aatggtcgga ccaactccag cttcgaatgc tgctgagtat actttaggag cgatgtatgc
     1321 aaatgaaatg ttgctttttt gcagtttgtt tgctgctgtg atctctgttt tacacgtggt
     1381 gagccaaacg agaaaaaaag aagaacttgg tctgattgaa cttattcgtt cctttcgagt
     1441 cggacgtcaa gcaaactctt tagctgtcat cgctgaaagt attctgatca atttgctttt
     1501 agcaatatcg atcagctgta cgatgatttt tttccaagcg gattcgatca ctgcaaaagg
     1561 tgcttggctt tttgggctat ctgtaggtag tatggggatt ttggggagtg ccgttgcact
     1621 gtttgctgca caactgatgc caagttcagc cagttcgaat ggtctgtctt tagcgttttt
     1681 aggtggactt taccttctac gagcaataac agatgtagct gattcctccc tttcgatgtg
     1741 gaatccttta ggctggtctt atttgaccta tccatttaca actaacaact ggattccttt
     1801 gctttatttg tttttgttca gtctaatctt gataatcagt tcatttgttt tagaggaaca
     1861 tcgagatttg aacaccggct atatccccga acgtgaagga aaaccgtatg ctaaacgttc
     1921 tcttttatcc ttgcgtggtt tattattcta tctaaataaa gggatgacaa tcagttggct
     1981 tatcactttt ggtttgatgg gggcggcata cggctctatc tacgggaata tgcaacagtt
     2041 tttagaaagt aacgacttaa tgaaacagat gtttatccaa tctggcgtct caattgaaaa
     2101 aaactttaca agcacgatca tggtagtctt gatttgtcta gctgctattt taccaattgc
     2161 tagcatcaat cgtttattta cagaagaaac ccacggacgt atgaatcagc tgttcgcaac
     2221 aaaaatttct aggaaccaac tttattggac caatatgtta ctagctattc tatttgctat
     2281 attggcaatc ctgattagtt ctggtgcact agggctttct gctttggctt cgatgaagga
     2341 tgcgaaagac atggatctat tgatgtttct taaagccggg ttcaatttcc tgcctgccgt
     2401 tttagtgttt atcggaatta gtggctttct attcggatgg cttcctcgaa tcggtaaact
     2461 tgcttatatc tacttagctt actcctttgc actcaattac tttggcggaa ttttggattt
     2521 accagaatgg ttttccaaaa cagctgtccc tagctgggtt ccacggatgc caaacgattc
     2581 ttttgatctt tccgtgttcc ttacgttatc atgtatcagc cttgcactgt tcgtccttgg
     2641 ttctattggt tataggcgaa gagacttact agaaggataa taaaaaagca tgtacaattt
     2701 catgctagcc tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt ttttatttac
     2761 aagtcttcta actgctcttt atcaatgatc cagacttctc gattctttct ttttacataa
     2821 ccattctcct ctaataattt aaattttcta gataaagttt cgggggtcgt accgataaat
     2881 gcagcaagtt ctttcatttt cattggcagg aaaaagtgat ccttccctgt cgctttagct
     2941 aagtcaagca gatagacagc gactcgttct tctacttttt ccatcatcaa aaaagttgtc
     3001 tgctgctgta cttttgttaa tttctcaacg ttgattgcta aaagtttcag actaagggcg
     3061 ggataatgca gtaagagttc cgagaaatct ttttgtctca atatgcaaat ttccgttttt
     3121 tctagtgctt cgccaaataa agactcattt ggaacgccta gcaactggct ctctccctca
     3181 tatccgcccg gctctattac tcgtaaaagc tgttcctttc cgctagcaga taattgatac
     3241 actttcatac tgccggaggc cacgatcact aattgaggtt ctccattcgg cgcaagaatc
     3301 tgttctcctt tttccacata gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc
     3361 tgtactaagt gattgaacaa aggaacaaat gaaacacata aatgatcatg actcataatc
     3421 tctcctcctt ctaaacagct atttctaata aagaaacata acttccctta ttccaactga
     3481 taattttctt ttatcatagc ataaacgtca agcgaaccag ataccagcaa atagagtgga
     3541 tttctttttg tcccttgctt tattttcctt cttttgagac cttaagtaat tgattcctat
     3601 caatttttta aatatttact ttttgaataa tcaaaaacaa accttcactc gcaagccgag
     3661 agtaaggttt gtttttcgca ttgcttattt cgtgttgtct attgaaattt ttccgcacct
     3721 agcttttgat atagtggtgc tagttcttgt aaggtttcag cggttatttg ctcaacgtgt
     3781 tcgatttctt gcggatagaa gaatagttta cccatttgaa aggttcggtc tctcatcgct
     3841 tttttcaact cttcttgttt ctttgtatcg gacaaatagt cagacaaagc taagtggtcg
     3901 tcaagttcat gttctggttg cggccatatg taataatcct tgatatcgac tgttttgctc
     3961 cactctttca gtatcgcaag caactggtta tacgctgcaa tgctaccata tcgttcttcg
     4021 cttttataat gttggtacat caaatagatt tggtattgtt tttgattcaa caaaactgct
     4081 agacaggcat tctcgtcttg acgagattcg cttcggtacg cgctccaaaa atgactgcgt
     4141 aaattccagc cattcgtcca gctttcgatt ttaggtttgc tcatgccgta catatcaggc
     4201 aaaagtgcag ctgtctggag ctgaatctct ttccattttt gccagtgttc tttatattct
     4261 ttcttgattt gttcaatctt atctgttgga tggctctctt tgagctgttt gaatgcgtag
     4321 attggtcgat caaacaactg ataatctgat tttccaagtt ccattgtttc gcctctttcc
     4381 gttatctttt gtattctacc tgaattttgc gatatggaca aatagaggtt gtgaaaaagc
     4441 tgttctgcat caagtcataa gaacagccaa acaaaaaact gcgatgagca tcaccgcagt
     4501 tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag
     4561 aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt
     4621 ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat
     4681 tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag
     4741 cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt
     4801 tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag
     4861 atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt
     4921 tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa
     4981 cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct agatcactga
     5041 gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt
     5101 gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct
     5161 gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag
     5221 aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga
     5281 cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag
     5341 gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat
     5401 ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac
     5461 gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga
     5521 atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag
     5581 ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg
     5641 ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag
     5701 tatacgtact attttcaacg gcgatatcat catctacgat cacactttct acttcaatat
     5761 cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct
     5821 taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat
     5881 ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta
     5941 caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt
     6001 ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca agttctggat
     6061 agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt
     6121 ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga
     6181 gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca
     6241 tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag
     6301 ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca
     6361 ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga
     6421 gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct
     6481 gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct
     6541 tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata
     6601 aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa
     6661 aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag
     6721 tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact
     6781 tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa
     6841 tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg
     6901 atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga
     6961 tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata
     7021 actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg
     7081 aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct
     7141 gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt
     7201 gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct
     7261 gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt
     7321 tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct
     7381 tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt
     7441 agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt
     7501 tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc
     7561 tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga
     7621 tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt
     7681 tcttttcttt tatt
//
LOCUS       DAXWLH010000126         7577 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXWLH010000126 DAXWLH010000000
VERSION     DAXWLH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.128"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(36..1001)
                     /gene="nrdF"
                     /locus_tag="ACGE36_002119"
     CDS             complement(36..1001)
                     /gene="nrdF"
                     /locus_tag="ACGE36_002119"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8384487.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1051..3210)
                     /gene="nrdE"
                     /locus_tag="ACGE36_002120"
     CDS             complement(1051..3210)
                     /gene="nrdE"
                     /locus_tag="ACGE36_002120"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8384488.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3369..3593)
                     /gene="nrdH"
                     /locus_tag="ACGE36_002121"
     CDS             complement(3369..3593)
                     /gene="nrdH"
                     /locus_tag="ACGE36_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFF8384489.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3994..4467
                     /locus_tag="ACGE36_002122"
     CDS             3994..4467
                     /locus_tag="ACGE36_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFF8384490.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4464..6596
                     /gene="feoB"
                     /locus_tag="ACGE36_002123"
     CDS             4464..6596
                     /gene="feoB"
                     /locus_tag="ACGE36_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFF8384491.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6598..6732
                     /locus_tag="ACGE36_002124"
     CDS             6598..6732
                     /locus_tag="ACGE36_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFF8384492.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6826..7470
                     /locus_tag="ACGE36_002125"
     CDS             6826..7470
                     /locus_tag="ACGE36_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8384493.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
BASE COUNT         2289 a         1380 c         1526 g         2382 t
ORIGIN      
        1 tatttttcga aaaatttttg aattaaaata aatccttatt ccaatccaat caaataatcg
       61 tcgtctttca tcgcttctac atgacctaac aaatagccgt ttccgacttg tgagaagaag
      121 tcatggttgc ttgtgcctgt tgaaatcccg ttcattacga ttgggttgac atcattggca
      181 gtatctggaa agagtggatc catccctagg ttcatcaatg ctttgttggc attatagcgt
      241 aggaatgtct tgacttcttc tgtccagccg attggatcgt ataattcttc tgtataacgt
      301 tcttcgtttt cgtaaagctc ataaagaaga ttgtacatcc agtcttttaa cgtttcttgt
      361 tctgcttcag gtaactcgtt gaatcctaat tggaatttgt aaccgatgta ggttccatgt
      421 acagattcat ctcggatgat tagtttgatg atctctgcta cgttagccaa tttgttgttt
      481 cctaaataat aaagaggagt ataaaaacct gaatagaaca agaatgtttc taaaaagacg
      541 ctggcaattt tcttttctag cggtgtaccg tgtttgtaga tttcattgat acgctcagct
      601 tttttttgca gataaggatt cgtatttgtc cagtcaaaaa tttcttcgat ttcttttttc
      661 gtattcagtg tactaaaaat agaagaataa cttttggcat gaacggattc catgaattga
      721 atattgttca aaacggcttc ttcatgggga gtgcggacat ctttacgcaa ctgatccatg
      781 ccactttcag attggacagt atctagcagt gtcaaaccgc caaatacatg tcctactgta
      841 tctttttcca attgagacaa tgtacgccaa tcatctaggt catttgaaag tgggatacgt
      901 gtatctagcc aaaattgctc tgtcagcttc tcccaagtgg atttatcaat gatatcttca
      961 atttcattcc agtttattgc ttcataatat gtttctgaca tgagaatgct cctttaatta
     1021 aaatatgaat aagcgaatct atctatggta ttagatcacg cagctttcac attgattgct
     1081 tccgatttct tctgcatcat cagtaaaggt acgtacgtaa taaatggatt tgattccttt
     1141 gtggaaagcg taatgtctta aaatattcaa atcacgtgtc gtttgcttcg ttgttgtctt
     1201 ccattcataa atgccttctg ggatttcaga acgcatgaat aaagtcaaac tcatgccttg
     1261 atcgacgtgt tgttgtgccg ttgcataaac atcgattact ttacgcatgt ccatatcata
     1321 agcagaagtg taatacggaa tcgtctcatt ggatagataa ggcgctgggt aataaatttt
     1381 accgattttc ttctcttggc gttcttcgat catgcgagtg ataggatgaa tacttgcact
     1441 cgtatcgttg atatatgaaa ttgagccatt tggtgctact gccaagcgat tttgatgata
     1501 cagtccatct ttcattactg cttgtttcaa agcattccaa tcttcagcag ttgggataaa
     1561 aatgccttca aacaatgctt tgactttttc tgattttggc tggaattctc cagttacgta
     1621 tttttcgaaa taagtgccgt cagcataagc tgatttttca aaattatgga aaacttgttt
     1681 tctttcacga gcgatattgt tgctttcaac aagtgtccaa taattcaaca gcatgaagta
     1741 aatatttgta aagtctaatg aatcctctga gccatattcc atttgttctt ttgcaagata
     1801 agtgtgtaag cccatggcac ctaatccaat cgtatgattc agcttatttc cgttttggat
     1861 cggtggtacg acatcgatat cagatgcatc tgtaataaat gtcaatgcac gagtcatcgt
     1921 acgcacagat tgaccaaagt tagggctttg catcaaattc acgatatttg tcgagcctaa
     1981 gttacatgag atatctgttc ctaatacttc atattcctgt ttgccgttaa tcacagaagg
     2041 cgtttgaact tgtaaaatct ctgaacataa attactcatg atgatcttgc catcgattgg
     2101 attctcgcgg ttcgctgtat caatattgat tacgtaagga tagccagatt cttgttgtaa
     2161 ttttgagatt tcattttcaa gatcacgcgc tttgattttt tgtttgcgaa tgtttggatt
     2221 tgcaactaga tgatcatact cagctgtaat atttacataa gagaatggga caccatattc
     2281 tttttcgaca ctatatggac taaataaata catatcttcg tttttacgtg tcaattcata
     2341 aaatttatca ggtacgatca cgccaagaga aagagttttt acgcgaatct tttcatctgc
     2401 attttctttt tttgctgaaa ggaaagcaat aatgtctgga tggaaaacgt tcaaataaac
     2461 cacgccagct ccttgacgct gacctaattg attagaatag ctgaaactat cttcgaataa
     2521 tttcatgact ggaacgacac cacttgcagc accctcatat cctttgatag gagcgccggc
     2581 ttcacgaaga tttgataaag taatccctac gccgccacca atacgggaca attgcaaagc
     2641 agagttgatt gagcgcccga tagagttcat atcgtcagtg acttgagtca agaaacaaga
     2701 caccaattcg ccccgacgtt tacgtccagc attcaagaaa gaaggtgtag ctggctgata
     2761 tcgttgatgg atcatttcgt ctgcaagagc aagtgctaat tcttcatccc cgttcgcaaa
     2821 gtaaagcgca ttgaaggcta ctcgatcttc ataagtttcc agataagcgg aaccgtcgtt
     2881 ggttttcaac gcatattgag aataaaattt gtaagcagcc ataaaagatt tgaaagaaaa
     2941 atgttgctcg tccaaaaatt gatatagctt ttcaataaat gatggtgaat acaattgaat
     3001 gaattctgct tctatatatt cttgatcgat caagtaattg attttttcca taatagatgg
     3061 aaaagtcatc gtatttggtt tgacattttc ctcaaaaaag gcaactaaag cttctttatc
     3121 cttattcaaa gggatttgac cattgactgg ccgattgatt tcattattca gcttgaaata
     3181 actgacatct tgtaaatttt taagactcat acgtacgccc aaccttcatt tcgtatttga
     3241 tgatgcttga ttgcaaatca ttgcattgtt tttaagcttt aaaaaaggat ttggaagttt
     3301 gcgacaaaaa ctggtgctgt ctcaagccct tcaaatccat gtatcacgtt ttgattcttt
     3361 tagtcatgtt agctggcaag tttacgcaac tgatcaggac ggaatccgat aactgtttct
     3421 gcatcagaag taatgacagg aacactttgg aatccttgtg ctttcaacca gtctactgct
     3481 tcaggctgtt catcgatatt gacttcttca aaagcaattt gattttctgt taaaaaccgt
     3541 ttgaccatct tacattgcat acaattattt ttagaaaaga tttttacgtt catggtgagt
     3601 gcctcctcta acacattctt attatctata atagatagta taaaactctt agtaaaaaag
     3661 tcaaacaaca aacactacat attgtgttgt ggaaactgtg gaatactaag ttttgtgttt
     3721 ttgaaaaaag agctgaaaac aagtatcgtg tgggaaaata gcaaatttaa tagagaaaaa
     3781 attgtggaaa actatgaaga aatatttttt tctcattttt atctatcaat actaaagatt
     3841 cgctcataat atcacaagta taattgacat tctcaattac cgagagtatt ctatgtgaga
     3901 atgattctca tttatttgcg taacacgtaa gaaaattaga acatataatg aatgatttta
     3961 atacatgatt tatttcacaa gagaggaatc aatatgcaga ccttagcgca ggcaaagcag
     4021 aatttcgttt atcaagtcga acgaatcgaa acagaagaaa aaacaaaacg ccatttgcaa
     4081 aatatcggga tcgttgccgg aagtaaaatc gtcgtcgtca atttatcagg caacaatgcc
     4141 attttactgg taaaaagtca tcgagtggca gtaagtcggg aaatactagg tcaaatcttt
     4201 gtcaataaaa agactgcttc ctctgaaagc tggacttctc ttgatcaatt aaaagcagga
     4261 gagactggga tcgtcgtagc aatccacgga caaggcgcag tgaagcgacg gttaatggat
     4321 atgggattga cgcgaaatgt aaaaatagtt atccgaaaac tagcccctct gggagatcca
     4381 atcgaactga cagtgcgagg gtatgaactg acgttacgaa agtcagaggc cgaactaatc
     4441 ttgatacaaa gggaggaaaa agaatgaata tcgcgttagt cggaaatcca aacagcggga
     4501 aaactagctt gttcaatctc ttgactggtt cgaatcaata tgtagggaat tggccgggcg
     4561 taacagtaga aaaaaaagaa ggatcgttga aaaaaaatcc gaacattcat attcaggacc
     4621 tacccggcat ctattctttg tctccttata caccagaaga agtgattgca agagattatt
     4681 tggttcagga acagcctgaa ctgatcatca acattattga tgctaccaat ttagaacgaa
     4741 atctttattt aactacgcag ttattggatt tagacattcc tatgatcatt ggtctgaata
     4801 tgatggatgt catcaaaaaa gaaggaaaaa aaatcaatct agaaaagctt tcctatggtc
     4861 ttggtgtacc agttttacca atcagcgtat tgaaaaagca aggaatcgaa aaactaatcg
     4921 aaaagattca ggagtctgca aaaaagcggc cttctattcc tgttccctgt acatatgatc
     4981 cacgtttaga agcagcttta catgaaattg aagatgtact aggagaacaa agcggcaaga
     5041 aacgctggta tgcaatgaaa tattttgaac gagatcagaa ggtaagagag gattacgaga
     5101 tagcagtttc tcagcaaaaa gagatcgagc aactgatcca gttaacagag aaactattgg
     5161 acgacgatag tgagacgatt ctggtcaatg aacgttatga atttattacc caactatgta
     5221 cactctgtgt tgtgtcaaat gattctttcc aattatcgat gagtgacaaa atcgatcaga
     5281 ttgcaacgaa tcgttggtta gccttgccga tcttcgcatt tgtgatgtgg ctgatctatt
     5341 atctggctat tcaaacagta ggaacaatgg ggacagattg gatcaatgac acattgtttg
     5401 gtacatggtt accagaacat gttggcagat tgttagctga atggcaagta gcgggatgga
     5461 tgcaggagct gatcttgaac ggcatcatcg caggcgtggg agcagtgtta ggatttctac
     5521 cgcaattgat tatattgttt ttatgcttga gctttctgga agactgcgga tatatggcga
     5581 ggatcgcttt tgtcatggat cgacttttca ggaaattcgg tctttcagga aaatccttca
     5641 ttcctatgct gattgctaca ggatgtggag tgccaggcgt catggccagt cgcacgattg
     5701 aaaacgagca agatcgtcgt ttgacgatca tggtcacaac cttcatgcct tgttctgcaa
     5761 agttgccgat tattgcctta gtagcagggg ccttttttcc tcatcaatct tgggtagctc
     5821 cttctgccta ttttcttggt atgactgcaa ttattttctc tggaatcagc ttgaaaaaaa
     5881 cacgtttgtt tgctggagat acagctcctt ttatcatgga attgcccgca tatcattttc
     5941 ctcaatggtc agcggtccta agacaaacct atgaccgcag caagtctttt gttaaaaaag
     6001 caggaacgat tatctttgtt tctagtatcg tgatctggtt cagctcttct tataattttt
     6061 atgctcaacc tgtttctgaa gaccaaagta tcttagcctt tttcgggcga atcctggcag
     6121 ttgtttttca gccgcttggt tggggaaact ggcaaggaac agtcgcagcg attacaggat
     6181 tggtcgctaa agaaaatatt attggcacat ttggtatttt gtttggtcat gcggaagtat
     6241 ccgaaaatgg cagagaaatc tggcaagtgc tacaacacac atatactccc gcagcagctt
     6301 attcattgtt agccttcaat ttactttgtg cgccttgttt tgcggcaatt ggggctatcc
     6361 atcgggaaat gaatgccaag aaatggactt ggatcgccat aggctatcaa tgcggtttag
     6421 cttatttagt tagttttgtg atttatcagt tgggacactt attggagggt ggacaggtag
     6481 cgttagggac gtatcttgct attttactga tgataggact cgtttatgtt ttgttccgtc
     6541 agccaaaatc caagaaccag ttttatacta tattgtcatt agaaggagag gaataggatg
     6601 gcgacattct tattaagtgt gttaatcttt ggaagtgcag gcgcaattgt ctatactcgt
     6661 ttaaaatccg gtaagaattg tgatgactgt caaacagctt gcccagtaaa aaaggaacag
     6721 atcaagcgat gatcagtcgt tgcaagaggc tattctcccc ttttttgctt attttgctag
     6781 agcatagtaa gatgagggaa agtaaaaaaa gaaagaggaa agaaaatgtc tggaaagaaa
     6841 aaaatcgtcc gcccgcgcta ccagcagatt gctgttgatt tagccgaacg tatcgttgaa
     6901 aaccgatata aagtgggtga gaagatccat gcccgctcaa cacttgccag tacgtacaat
     6961 gcttctcctg agactacccg caaggctatc aatgtgctgg tagatcttgg aatcatggaa
     7021 gtacggcatg gaagtggggc atttgttgct tcgaaggaaa aagcaaaaga tttcctattg
     7081 caatatcaag atgtacaatc catccaagat acaaaagcag aaatcatggc aagtgtggag
     7141 aagcaacaag atgagctgaa tcatttttct caattattgg atcagctggt caatcaaacg
     7201 aagcgaatac ataagatgaa tcctttactt ccgtttgaat tatctttatc agaaaaagca
     7261 atgcatctgg accgttcaat tagcgagttg aatatctggc aggcgactgg cgccacagtt
     7321 atcgcaattt ctcataatga agaactgctt gtttcacctg gcccctatgc taaatttacg
     7381 gcaggggaca tcgttcattt tattggaaat gaatatacac tacagcgtat gacaaacttt
     7441 ttttacccgg agcatcagct tgcagaatga ttgtgtgaaa tattaaaaaa atattaactt
     7501 ggctaaacct tttatttaca aagatttagc caagtttttt tcagtttgga caagcaaatt
     7561 tctttcattt ttgacaa
//
LOCUS       DAXWLH010000127         7509 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXWLH010000127 DAXWLH010000000
VERSION     DAXWLH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.129"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            9..770
                     /locus_tag="ACGE36_002126"
     CDS             9..770
                     /locus_tag="ACGE36_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFF8384494.1"
                     /translation="MNQPQIFNFEHNEVRTFLENDVPYFVANDVAKTLGYKNPSKATN
                     DHCKKAIETWGNDSLGRRQKFKIIPESDVYRLIIKSNLPSAEKFESWVMEEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQQVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            901..1041
                     /locus_tag="ACGE36_002127"
     CDS             901..1041
                     /locus_tag="ACGE36_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384495.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            complement(1038..1208)
                     /locus_tag="ACGE36_002128"
     CDS             complement(1038..1208)
                     /locus_tag="ACGE36_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384496.1"
                     /translation="MNKKIAADLVVAWLNHESQISTKGESISPKEIAQAYLDIQYAVI
                     YGQLPEDRKNDD"
     gene            1288..1479
                     /locus_tag="ACGE36_002129"
     CDS             1288..1479
                     /locus_tag="ACGE36_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384497.1"
                     /translation="MDTQRIVLETEEKEFIETVISFVTEKGWTISNLQNAVSKVEDYM
                     KKNATLTELTIGMPAQIKD"
     gene            1553..1780
                     /locus_tag="ACGE36_002130"
     CDS             1553..1780
                     /locus_tag="ACGE36_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8384498.1"
                     /translation="MTQELINKNDLDSFLIGYVPKRYLTQKEAVHYTGTSAGTINEWV
                     KKGLKVIIFGENSRPKYDIKDIDEFMSKYKV"
     gene            1793..1966
                     /locus_tag="ACGE36_002131"
     CDS             1793..1966
                     /locus_tag="ACGE36_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384499.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(2080..2487)
                     /locus_tag="ACGE36_002132"
     CDS             complement(2080..2487)
                     /locus_tag="ACGE36_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384500.1"
                     /translation="MSFDNTITISIILALVALISPWITAVINNKHAESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKILPILNKSLEKL"
     gene            2649..2873
                     /locus_tag="ACGE36_002133"
     CDS             2649..2873
                     /locus_tag="ACGE36_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384501.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            2883..3077
                     /locus_tag="ACGE36_002134"
     CDS             2883..3077
                     /locus_tag="ACGE36_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384502.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDVILEYVQQHYPKIELGGI"
     gene            3080..4102
                     /locus_tag="ACGE36_002135"
     CDS             3080..4102
                     /locus_tag="ACGE36_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8384503.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGENLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            4065..4880
                     /locus_tag="ACGE36_002136"
     CDS             4065..4880
                     /locus_tag="ACGE36_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFF8384504.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKTFTPIIAAVSKQTPSEARLITI
                     DQDNINYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            4896..5681
                     /locus_tag="ACGE36_002137"
     CDS             4896..5681
                     /locus_tag="ACGE36_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384505.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNDKNDKNDKNDNNPRNSRKTREYAD
                     DDPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSND
                     FWSGVILSPTSLRKHFDKMAIQKNKRKQQNISNDELPETGEDW"
     gene            5681..6529
                     /locus_tag="ACGE36_002138"
     CDS             5681..6529
                     /locus_tag="ACGE36_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8384506.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            6526..6768
                     /locus_tag="ACGE36_002139"
     CDS             6526..6768
                     /locus_tag="ACGE36_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384507.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            6911..7279
                     /locus_tag="ACGE36_002140"
     CDS             6911..7279
                     /locus_tag="ACGE36_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFF8384508.1"
                     /translation="MATRSKYGNKKHEVDGITFDSIAEAEYYDLALWQAEANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHK
                     YQVPLILAKKYRNTFKEERF"
     gene            7294..7455
                     /locus_tag="ACGE36_002141"
     CDS             7294..7455
                     /locus_tag="ACGE36_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8384509.1"
                     /translation="MTTEEVIQMHIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT         2808 a         1233 c         1582 g         1886 t
ORIGIN      
        1 gtaaaaaaat gaatcaacca caaattttta atttcgaaca taacgaagtt cgaacgtttc
       61 tggaaaatga cgtcccatat tttgtagcga atgacgttgc aaaaactttg ggatataaaa
      121 atccaagcaa agctactaat gatcattgca aaaaagcgat tgaaacatgg ggtaacgatt
      181 cgttaggtcg tcgccaaaag ttcaaaatca ttccagaatc agatgtttat cgcttgatta
      241 tcaaatcgaa tttaccaagc gctgagaaat tcgaatcttg ggtaatggaa gaagtcctac
      301 ctcaaatccg aaaacacggc atgtacgcaa cagatgaact acttaacaat ccagacttgc
      361 taatcgaagt agcaaccaaa ctcaaagaag aacgcacgtt acgattgata gcagaacaac
      421 aagtaaatga attacagccg aaggcagact attacgaccg aatcttgaac aacaaaggat
      481 tagttacagt tagtacgatt gctaagaact acggtatgag cgctgtatcg tttaataaat
      541 tgctacatga attaggtatc cagttcaatc aaagtggcac atggctgcta tacagcaagt
      601 tccaagacaa aggatacacg catattgagc catttgatta tgaagataaa aacggcaatc
      661 ggcaagtaaa aacaaggatg aaatggacgc aaaaaggaca cattttctta tatgaaacat
      721 tgaagaagaa taattactta ccgatgattg aacgtgaaca aacagcatag gaggtgagat
      781 gaatggagag accaaaaggg cgcgtaacca ttgacctaaa aattacaaaa accaactcaa
      841 aaatgagctg gcttcataga ctattctttt ggaaagatat aaaagaaagg agagaagaat
      901 atggaaaaaa cagtctcggc aaaaatcgaa gatttgaaat tagacatact gaaacaagca
      961 aaagtggcga tggaacacgc ggtagataaa gaagactctg ccatggttgc agccatagca
     1021 gagattttag ctcacgttta gtcatcgttt ttacggtctt ctggaagctg accatagata
     1081 acagcatatt gaatatctaa ataagcttga gcaatttctt ttggcgaaat tgattcgcct
     1141 ttagtagata tttgtgattc gtgatttagc catgcaacaa ctaaatccgc agcgattttt
     1201 ttattcatac ttattcacca ccttatgcat tatttcagta ggccacttac tgataagaaa
     1261 attatatcaa agaaaggaat gaatagaatg gacacgcaaa ggattgtttt agaaacagag
     1321 gaaaaagaat ttattgaaac ggttattagt tttgtaaccg agaaaggctg gacgatttct
     1381 aatttgcaaa atgctgtatc aaaagtggaa gattatatga aaaaaaatgc cacattaaca
     1441 gagctgacaa ttggcatgcc agctcaaatt aaagattaat agattctgca gaagcaaaca
     1501 aaattataga ttcgattatt actatgtacg aattgtagag ggggaaaacg aaatgacaca
     1561 agaactaatc aacaaaaatg atttagattc atttcttata ggctacgttc ctaaacgcta
     1621 tctgactcaa aaagaagcgg ttcattatac aggaacgtca gcaggaacta ttaacgaatg
     1681 ggtaaaaaaa gggttgaaag taatcatctt cggtgaaaac agccgtccga aatacgacat
     1741 caaagatatc gatgaattca tgtcgaaata taaagtctaa ggaggtaagc ggatgggaaa
     1801 atttaataga gcattagtgt tcagcgcacc cctaatcgtc tatgctttag gactttgggg
     1861 aagcagacaa gcattaatcg gcacgatcgt ttacatggtt tggattttta tggggcttga
     1921 tgaagctgag tacagagcaa aaaagccaac cgagggggct gactaaagtg tggttctgtt
     1981 tattaggtgt ttatttcgtg gccgttttag taaataacta catgagaaaa cgtggcgaat
     2041 actggtatac atcctatgcg gttctagtat gtctaatgct tacaattttt ctaatgattt
     2101 attcaaaata ggtaatattt tgtcaatcaa ataagtttca agagattttt gttctgcatc
     2161 agtgttgccg taaccaaaac ggcttaagaa aattttcata acctctatat cttcattttg
     2221 aatatatggc aggactgcat aacctgatgc ttttacagca gatatatttt tatcagtgtt
     2281 actaccaata caaataccca cattgttgag aaatgttgag aaagttgttt gtattgtttg
     2341 ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc ttcatcgatt cagcatgctt
     2401 attatttata accgctgtaa tccaagggga tataagagct actaaagcta ggataatcga
     2461 aattgtgatc gtgttatcga aactcatttt ttcaccacca gttttttacc taaattatat
     2521 caaaaaggag agaagaaaaa atgcaagaat tagtaatttt gaaaaataaa aaaagacacg
     2581 aacgccggca agcatgatcg tgtccaaaag aaaaatcttt taaaccatta taacacagga
     2641 ggaaataaat gatctcagtt aaagggttag gcgatgaaat attcgaagca atgatgagca
     2701 aagcacaaca ggacatcaaa gaaaaaatca tttctgctgc agcgcaagga caaactagct
     2761 gcactgttcg atcgagagga acgacaccaa gttttctttt ggcgctagaa gatgaaggca
     2821 ttttgaatat caaacgaaga gatggcagcg tcagattgtt ttggcaattt taggaggccg
     2881 tgatgaaaga ctttgattct ttaggtgcca gacagcaacc accaaatgaa gctagtcctg
     2941 tcggagttga ttggcaagaa aatccgttat atccaggtga tacttgctat ctaacagaag
     3001 aaggttatgt accagtagac gtaattctag agtacgtaca gcaacattat ccaaagattg
     3061 aattaggagg aatttaggaa tggcaaatga tttaacacaa acaacacaac gttcattaga
     3121 tgagcaggta atcagtaatc ttggacggtt acaagaacaa ggtttagaaa tgccaccagg
     3181 atatagtcca caaaatgcgt taaaaagtgc ttttttcgaa ctcactaaca atacaggaga
     3241 aaacttgttg cagatggctg caaacaatca agaaatgaaa acctccattt ctaatgcact
     3301 tttagacatg gttattcaag ggttatctcc agcaaaaaaa caatgctatt tcattaaata
     3361 cggaaacaag gttcagctaa tgcgttcata ttttggaaca atggcggtcc ttgatcgtgt
     3421 aactggaggg gcagatatta cgcctgtagt cgttcgacaa ggtgatgagt ttgaagttgc
     3481 aatggatgga ccaaacatgg ttgtcaaaaa gcatgaaaca aaattcgaga atttagacaa
     3541 tgaaatcatt gcagcctatg tagtcatcaa gttaacaaat ggtaaagaga ccacaacagt
     3601 catgacgaaa aagcaaatcg atcaaagttg ggctaagtca aaaatgaaag gttctggacc
     3661 gcaaaaggaa tttccagaag aaatggctaa acggacagta atcaatcggg cagctaaaac
     3721 attgatcaac acaagcaacg acaacgattt attagttcaa gctgcaaaag atacgttaga
     3781 aaatgaattt gacaatgatc ggaaagatgt aacgccacaa acagaaaaag tggctactct
     3841 cgaacaaaaa ttcttttcaa acaaaaagat tactgaacca atacaaaaag aaccagatcc
     3901 gattgtgatt ccagatgata tccaaaatga agtaactcga gttgctgatg tacctgccca
     3961 tccagaaata gagcaagcac atttgatcga aaatgaggat atcaatccaa ttcaagaaga
     4021 attgatagat attccagact ttggacgcga ggaaggtgta gaagatgtct cagaatttga
     4081 agacgacgag taccctttct gatgagaatt attactccaa tgaagctgac tggcagtaca
     4141 tgtcaacatc acaatataag tcctttttaa aatgtgaagc cgcagcctta gcaaagttga
     4201 aaggcgattg ggcaccgacg tcggatccca aagccttact cgtaggaaac tacgtacatt
     4261 cttattttga atcaaaagaa gtacatgaag cgttcaaaga agaaaataaa tccaaaatgt
     4321 tttctagtag gaagccgtat ggattgttga aagatttcca aattgctgaa caaatgattg
     4381 aacgacttaa gcaagaagaa gcattcatga acatttatca aggtgagaaa gaaacgatcg
     4441 tgactgggga actatttgga acgacatgga aaggcaagat tgattgcttg aatgttgagg
     4501 atgagtattt tgttgatatc aaaacaacca aagacatgca tgagcggaaa tggaacgaaa
     4561 actacggatc aagagaaacg ttcattgtca attttggtta cgtacttcaa atggcaatct
     4621 atcaagagct tcttttacag caatacggaa agacgtttac accgatcatt gcagcagttt
     4681 ctaaacaaac accaagtgaa gcaagactga tcacgattga tcaggacaat ataaattatg
     4741 agctggttat gttaaaagaa aaaatcgaaa gaattgtcag agtgaagaac ggtgaagaga
     4801 agccgaacca ttgcggtttg tgcgagtact gtagaggtaa tcttccaatt actggattca
     4861 ctagcatgga cgatttgtag agtggtggtg atgaaatgac tggaggatgg ataaagcttt
     4921 atcgccaatt actcgagaaa cctatttggc aagaatctac accagagcaa aaagttattt
     4981 taattaccct tctttcaatg gcgaatcatg aagaaagaga gtgggaatgg caaggcgaac
     5041 cgtacaaagc aagtccaggt caattcgtta cttctctcga tagtattaga aaaaaatgtg
     5101 gtaaaggtat ctcattgcaa aatgttagaa ctgctctaaa gaggtttgaa aaatatgaat
     5161 ttttaacaaa ccaaccaaca aacaaaaata ggcttataac cattgtcaac tgggaggttt
     5221 accaatcaaa agacaacgat ctaacaaagg aactcacaag caaccaacaa gcaactaaca
     5281 agcaactaac aactaacaag aatgataaga atgataagaa tgataagaat gataataatc
     5341 ctcgcaactc tcgaaaaaca cgagagtatg cagacgacga tccaaataaa aaattggcca
     5401 ttcttttatt aaaactcatt cgaaaaaatc aaaacatcaa ggaacctgat ttggataaat
     5461 gggcgaatac gattcgttta acaattgaat ctgacaaacg aactggtaga gaagttcaag
     5521 acatgatcgt gtgggccaca agtaatgatt tctggtcagg tgtgattttg tcaccgacta
     5581 gcttaagaaa gcatttcgat aagatggcta tccaaaaaaa taaaagaaag caacaaaata
     5641 tttctaatga tgagttacca gaaacaggtg aggattggta atgaataaaa aactaagtgc
     5701 aatggctgca ccttacggcg gattaaaaac agcagatcat aattgcccaa aatgtggtga
     5761 tccattgtat atctggaaaa caaaaaataa agatggtact gatcgatgcg gtcctacatg
     5821 tatcaataag atttgtggtt accgagaaat ggtaactaaa aatcaaaaag aagctatcca
     5881 aaaagcgaat gaagcaatga aaaaggacgc tatcaatcga atgcttaaca gttcaatgat
     5941 tacagatgat gccatatgga ccttcgattt tgatggatac aaagtagttg atcaggaaac
     6001 agcacaaata aaagcgatgg ctcaagaatg ggccaaaaaa attgtaaatg gtagcacgat
     6061 ccatgcagtg ataaccggta gaacaggagc tggaaaaact catctaggcg ctgcagtgat
     6121 taaagaagtc atgagggcat ctaattataa aattgcttgt tcatttatca gttatcgaga
     6181 acttttagaa cagttgaaat ttgcaatgaa tgatccagaa gcaagaaaag ccgtaacagg
     6241 ttcgctgatg gctgagataa agaaatcaga tttcgtggta atcgacgatt tgggtgcaga
     6301 actaggaaga atggaagaaa acaatcaggc aacgccttat gatgttgatg ttctcacatc
     6361 gctcacagaa gctcgtctaa acaaagctac gatattcaca accaatttat catcgaagca
     6421 attaaaacac gcgtacggtg agcgagtgtt ctctcgtgta atgaatggaa cgaaagggaa
     6481 catagctgta ttcaaaacaa cgacagacaa aaggaggaat ccagtttgac ctttgtagtg
     6541 aaaaaaatgt gctacttaga tagccgaggg cgaggagaag ctagtgtcga gtttgctaaa
     6601 caccacacga ctaaagaaga ggctgacctt gttgcaagtg tttgtggcgg cgaagtagtc
     6661 gaagttatta aacccaaaag acgattttca aaacctaaga caaagccagt gaaaaaagag
     6721 tgttgttgtc cgaaaagtaa ccaagcatgg atgagaggtg caaaatgact tgtttaaaat
     6781 gcaacgatga aacagttatt tggtataaga catcgcttgg atggtcaact tgtgaacctt
     6841 gtccaatatg caatgaaaat ggacgacgtt cgaaagaacg actcgaaaga ctaaagaagg
     6901 agtatagcaa atggcaacaa gaagcaaata cggaaacaaa aagcacgaag tagacggcat
     6961 cacttttgat tctatcgcag aagcagagta ctacgatcta gccttgtggc aagcggaagc
     7021 gaatggctgg aaagtgaaac ttcaggaacg atttgagctg atgccgaaat ttgaactaga
     7081 aggaaagaag tatcgcaaga tcgagtatat tcctgacttc acattttata aaaacggcaa
     7141 acttgtcaaa gtcgtagatg tcaaaggaat gcagacaaaa gactttaaga tcaaggcaaa
     7201 gttgttttgt cataaatatc aagttccgtt gattttagct aaaaaatatc ggaatacgtt
     7261 caaggaagag cgtttttaac gaggtggtcc atcatgacaa cagaagaagt gattcaaatg
     7321 catattcgaa gccttcagcg tgagattgac gatctggaac gaacaaaggc agtgatggtc
     7381 aatgaaacgg cgagaaaggc aatcgatttg cacatagaaa atttaagaag ggaaattcgt
     7441 agattggagg aatgagcgtg gataagaaag cagcaatgaa acgaatcatt gaactgacac
     7501 attctgaga
//
LOCUS       DAXWLH010000128         7466 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXWLH010000128 DAXWLH010000000
VERSION     DAXWLH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.130"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(354..524)
                     /locus_tag="ACGE36_002142"
     CDS             complement(354..524)
                     /locus_tag="ACGE36_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384510.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(539..1072)
                     /locus_tag="ACGE36_002143"
     CDS             complement(539..1072)
                     /locus_tag="ACGE36_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8384511.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1093..2037)
                     /locus_tag="ACGE36_002144"
     CDS             complement(1093..2037)
                     /locus_tag="ACGE36_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8384512.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2674..2760
                     /locus_tag="ACGE36_002145"
     CDS             2674..2760
                     /locus_tag="ACGE36_002145"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8384513.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(2881..3570)
                     /locus_tag="ACGE36_002146"
     CDS             complement(2881..3570)
                     /locus_tag="ACGE36_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384514.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3815..4729)
                     /locus_tag="ACGE36_002147"
     CDS             complement(3815..4729)
                     /locus_tag="ACGE36_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8384515.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(4711..5091)
                     /locus_tag="ACGE36_002148"
     CDS             complement(4711..5091)
                     /locus_tag="ACGE36_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFF8384516.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(5541..5828)
                     /locus_tag="ACGE36_002149"
     CDS             complement(5541..5828)
                     /locus_tag="ACGE36_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8384517.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(5848..6072)
                     /locus_tag="ACGE36_002150"
     CDS             complement(5848..6072)
                     /locus_tag="ACGE36_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFF8384518.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            6275..7453
                     /locus_tag="ACGE36_002151"
     CDS             6275..7453
                     /locus_tag="ACGE36_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFF8384519.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGAKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT         2071 a         1401 c         1293 g         2701 t
ORIGIN      
        1 taaaaagtag ttttgaattc ataatcgttt aaataaaatt ataagatcag ttatttaaac
       61 agctagtaga cataatgtgt atactaccaa gtaacgcctg taccaaggga tccaggcgct
      121 gattttgtga atttttattg gtctaggtta aaagctcttt ttaaggcttg gttttctgcg
      181 gttgtttctt tctaattctt tgtgaatatt gatcggtcat tttttaaata cttgttattt
      241 ttgtttgtgt ttgttatgct ttttacatat ctcatattca tttattattc ttttctaagg
      301 ttaataatag gataatctct ttcagaaaga ctgctcgtac gagcagtctt ttcttattta
      361 tcaagttttt ctacaaatac ttcctcattt tgactgttgt agtaattagg attgagtttt
      421 agttcccctt ttttatttcc tttgttggcc actgataata acattgggct aaagcttaat
      481 attcctagta ggatagaacc gaatagcaag tgtttttttg tcataagtat ttccttcttc
      541 atttccatag ctgccaccat tttttccaat tctttgtttg ttcgtccatt gtttctgttg
      601 cttcagaaag agtcgttgaa ctattctctg aagactcttc cgtataagtc aactgcagtt
      661 gctcctgaag tttttcgatt tgttggttgg cttgcagggt gagttgttgt tgctggtcga
      721 gtagtttttg tatatgagaa ttattttgct ctttgagggc aagttgttct cgtaaaaatt
      781 cattttcttc acgcaactga tctagcaact tgtttgcaac cacgtttttg tttggtatat
      841 aagcattttt atcgttgttt tgttcatgtt tatgcaactt atctagcaac ctttgattgt
      901 ctaagttgcg agcaagttgc aatactccag aacgcaatac aaccttataa ccacgctcat
      961 ttttagctat catggaagaa tctaattttt ggatatgttt cgatattgct tgcctactca
     1021 tcttaaaaag atctgacaat tcctttattg taaaggtttc ttgtggttgc atactggttg
     1081 cctcctaggt tgctactgga ataaattatc taaatcagat aataaacttt ttttctcttt
     1141 ttccgaaagt gcggactctg tttgtttttc aagagctttt ttctcttttt ctagttgttt
     1201 gatttgttgg aggagttcaa gttctcgttt cttctgtttg attttttcgt taattgttgt
     1261 agcgggttct tcgtattctt tttgtttatc agctaaagcc aatgctttat tttggtcttc
     1321 tttggttggt gcatgtttgt taaagtcgtc tgcttttttg atctcaggtt tgaacgtaaa
     1381 ttgatagcca ataacaggtt ttccccgtcc ttttccgtat ttctttttga ttgttagtcc
     1441 tttgaagatt gcagttagtt cttgacgtat aggttttata actcgtttat ctatatcagg
     1501 ttgcttataa cttttaggaa tatctaataa ttcaagaaag tcttctttgg agaaatatgc
     1561 gaaacctttg gttctgtatt gctttaacag acgaaacata gtctttgcat agccactttg
     1621 tagctcgttg aattgttgta aagaatagcg tacccactcg tctaaattat ttaacaaagg
     1681 cagtgctttt tcgtaaattc gtatatctac gtaaggattt tctacgtccc cgttgatttt
     1741 aaattctgta aacattacaa acatatcgta agaaagccca gactcacttc tacgaccaaa
     1801 gcgtaaagat aagatttttt cataagttgc ttttaaatct tctacaaatc gtacatttgc
     1861 agttgctttg tagttactta aatcttttag ttggtcaaac gtaaaacgaa ctgtcttatc
     1921 ccctttttca cgcattctag acacgatgga aaaaaacaaa ttcatttcaa ccggtgtaaa
     1981 ttttcttaaa ggaattgtat ttaattcatg gtggtatttt acaatctcat tagccatata
     2041 atcacctcaa tccaattatg aatgacattt tttaaatgtc aagacattca tatttgacgt
     2101 tcaaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac agacaaaatg
     2161 tccttcataa acagacaaaa tgtccttcat aacattctgt atccattggt agagtaagga
     2221 cacagcttac ggtaaagagg tttaaaaaga ggtttaaaaa gagatataaa aagagggggg
     2281 ctaattttcc cgcctaaaaa ccacttcttt tttctttaaa caggttcgcg catttttcaa
     2341 aactgccaat ccaaaacctt gcttcctacg gtcgccaaaa aggtgtgatc gctacgctct
     2401 cacattgtac tgcgcagtcg cctaaaggct cctttgctaa aaacatgttg gtaaagaggc
     2461 ctttgagcgg ttctgaatgc ttatctaagc cgttttaaga cagtcttgga taaaaatcct
     2521 tatctagctt taaagcgcct tacaggcggt cagggcaagc cctaacaacc ctaaaataag
     2581 cgatacttaa aaacaccaca caagtttaat tttttaagat ataataactt gtcgcctatc
     2641 cgacagtctt gcgactggaa ggaggtgtat ctaatgtttg aattgttcat ttgccccctt
     2701 cttatagaag tttgtgtctc tttattcagt tattggttga ataaccgaaa caagaagtaa
     2761 aatcaataaa ggcgacatca acctgaaaaa aagccccaga gaatccgacc tctctggggc
     2821 tttcgtgtat ctaatgtttg atcatttgca tgtttagttt aacatgattg ccgatcaaat
     2881 tcaattttgc tgttgctgat ctaaccattc cttgacactt tttagataat ttaatgccgt
     2941 ttcttttttc gttggatttt gtatgttcgt tgcttgctga agcagtgctt tataacgttc
     3001 gtaggtaact gattgttgcc gttcttttcg ttcctgttca ctagttgaaa aagggattgt
     3061 atcactgaat aaattataac aagcttcttc tgtctgcgta tctttaaaat attgcacgtc
     3121 ttttttggtt cgctcatagt tgtcttgaac ctctttttgc tgtttttctg gcaagttttg
     3181 ataaagacga gtgagttttt ccatgttttt atccgtcaaa cgtttttctt gattcacaaa
     3241 aatatcgttc atttcctcat aacaagtggt aaactcttcc attttttgtg ttaacacttg
     3301 ctcctttttc tttttatcac atgctgcttc atacttttga gtgatatccc ctaataaaat
     3361 acttcttgaa atacgtttat ttccctctaa aacgccgaat aaaccactta aaacgattga
     3421 taatgtaaat ataacccctg gcaataaaaa cctcttaaat cgaattttag agccttttaa
     3481 tgaatatctt gtttgataag ccttatagct aagccaaata attcctaaaa tactggcaaa
     3541 aatgagtaga tagggtaaat agaatgccat ttctatttat tctccccata ccagccaaaa
     3601 ggctaacagt gtcatgatta agcaacataa gggggcaaga atatccattg atcgctgcat
     3661 aattactttt ttctgttctt tatttcgctg gtttaatagc gcatggtgga attttttgac
     3721 ttgtgaatca attgtagttt gtattctttc tttggtataa ggtgcaagaa aagcacgttg
     3781 tttctttatt tcattttcta tgtcttgtaa cgttttagaa cgataattct tgttggtagt
     3841 ctgctcctct ttcaacgtgt tggtcatggt ttcttcgagt cggtctaatt tccggttgtt
     3901 cagtcgtaag agttcgttgt tctcttcgta cttcttttgc agtttcgcga atgctagctc
     3961 gagctcttcg tagctcgttg gttcgttgtt gattgtaggc tcgttctcgt ctgtccagct
     4021 catgtaaaat cgtctccttt tcgtaatcct ttcctagttt gttggatcgt acttttttct
     4081 ttttgtggat caattcgtaa gtaaaatttt tcccacgatt caagcaatga atgccgaatg
     4141 ttttaagaga atcacaaaat tggttatacg ttcgagtttt tttgtttttc attgtttgat
     4201 caatcgcaaa ccgaatttcg tctttccaaa caggttcatt tttttctttt aatttgattt
     4261 cagacatggt tcgtttttcg ggtttcttcc ctcgttcagt gattgttaag tcatgttcaa
     4321 gacaaatctc gtcatttagg tcataacatt tgtttaaaaa tgcttgacca tgggcttgaa
     4381 atttgttccc agtttccaga ttgacggagt taatgacaat gtggttatga atatggtctt
     4441 tgtctgtatg tgtgtaaatc gcgatttggt ggtcaggggc tatttttttc gcatactctg
     4501 cccctagttg attggctttt tcagcggtaa tttctcctgg tcgaaaagac tggatcaata
     4561 agtgtgcttg tacacgatcg tcttttccat acagcatgcg aatttgtttc atttcgcttt
     4621 tggcataatc aatgtcacag tttatccctg ttttgacggt tgctcgtggt tcggcgtaat
     4681 tgatcgctcg gctacaagaa gtggttcggc ttactttaac tgttgccata tcttttgtag
     4741 ctccttttgc atgattttta ggttttcgga taagcgttgg gcgtcgccag gatcaacgtc
     4801 gtgttggtgt gtgttgcacc acttggcaat ttgattgata ttcccgccag ctttggctaa
     4861 atcgcgcgcg attgcctgag caatgtctgg cgccactttc ggtgtcacca gacgcgcgtt
     4921 ttgtgccttg gctttcacga aagccggcac actcatttgc aaagcttcag ctgactgctt
     4981 cagctttgca aattcggatt cgctcactcg aaaactgatt tgttttggct cggatcgata
     5041 ggtatatttt ttagaatgat tgctagccaa atattgattt tgttcgctca taagtcccac
     5101 cctttcagta cgtcctacgg acgtatcgtt cgcaatacca ttgtacctaa aaaggtacaa
     5161 taagtaaagc tcactaaagc tcactaaagg gcttggcaag ctctatattt gctagccaaa
     5221 tacctacgta tttggtgcaa ataaccgctt gtcaaagggg cgcaagctcc cccttgaatc
     5281 cccctccccc ccactctcca caatacaaaa aagactagca aatgctagcc actttttgta
     5341 aagtttcgct tgtttcacct tcggtgaaat tgttgcaaat caataattag gtagagctac
     5401 atttttttcg ttgttcaaag attcttatgc catttactat taataaatct ataaatatga
     5461 taatttttcg gctctataat ttatgggact gatgaatcag aattgattct tcagtcccat
     5521 tttcatttta ttccaaagaa ttaactccaa ataccaatag aagcccatcc tctatatact
     5581 tgagattcct tactaacaat ttcttgtaat tctattaaag atttaggtaa tccaaattta
     5641 tgttttaaaa tatatcttcc aagaaaacta cttaatttca tactaacttc accaacactt
     5701 tttccctcac tgatttcttt agaataaaga aagagctttt cttttaagtc aggatagtca
     5761 tctatagttg cttcattgta tactttacta attgcattta aaagtttttc ttctttagta
     5821 aaatccatta taataacctc cctaccacta ccttctaggt gcccatggac catgattaac
     5881 ccaaccatta acaataattt ttccaatttc tcctttagct tgattccaat ctacccaaca
     5941 tttttctttg ttacaataaa gaccatttcc atagtaagta gctgcatcaa cttttattcc
     6001 tgtaccgatg ccagctagac aagttcctaa tattcctaga ataacacaat gttttaatac
     6061 tttctttttc atgattgttt ttccttttcg tttttttaaa attgcttgtt atattaaata
     6121 atcttagtaa ttttattata taacatttta ttatataaca atgattaacg tcataaaaag
     6181 gagagtgtaa aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt
     6241 accacaacac attcacagaa aagaggactt ccatatgaac gattttacta cagaaattct
     6301 aaagactcta gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggaaaaagc
     6361 tgtcaatacg cttctcaaaa cggagttaac ggctttcctc gattacgaaa agtacgatcg
     6421 tattggtttt aacacgggta attctcgtaa cggctcctat gaccgtacgg ttaagaccga
     6481 gtacggggaa cttcatctcc agattccgcg cgaccgcaac ggcgagttca agcaacagac
     6541 tgttcctgct tatagacgga cgaatgatac cttagaggag accgtcattc acctcttccg
     6601 aaaaggtatt accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcatcacta
     6661 cacgccccaa accatgtcca acatgactaa agtgctgact gaagaagtaa atgcatttaa
     6721 agttagaact ctaaatgata agtatgtcgc tattttcatg gatgctactt atattccttt
     6781 aaaacggcaa accgtctcca aagaagcaat ctatattgcc attggtatac gagaagacgg
     6841 cgctaaagaa gtactaagtt atgcgattgc tccgactgaa tcgacctacg tttggaatga
     6901 gttgttacag gacatttact ccagaggggt tcatgatgtc ttactgttta ttaccgatgg
     6961 cttaaaaggc atgaaagata cgattcacca gatttatcct aaagctaaat accaacattg
     7021 ctgtgttcac gtttcccgta atattgctca taaagtacgt gtcaaagatc gaaaagaaat
     7081 ctgtgatgat tttaaggctg tttatcaagc tagctcaaag gaagaggcaa ataccttttt
     7141 agggagtatg attgagaagt ggcagaaaac ttatcctaaa gtgacgcagt cactgataaa
     7201 aaatcaagat ttattgactt tttatgagtt tccgcctggt atccgccgaa gcatttactc
     7261 aactaattta atcgaatctt tcaataaaca aatcaagaaa tacagccgca gaaaagagca
     7321 gtttcaaaat gaagaatcac tagagcgttt cctagtatcc atctttgata catacaatca
     7381 aaaattctta aatagaagcc ataaaggctt ccaacaagtg acggatacat tagcttcaat
     7441 gtttactgag taacccatta ttttgc
//
LOCUS       DAXWLH010000129         7399 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXWLH010000129 DAXWLH010000000
VERSION     DAXWLH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.131"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            245..892
                     /locus_tag="ACGE36_002152"
     CDS             245..892
                     /locus_tag="ACGE36_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8384520.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    1021..1237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1285..3930
                     /locus_tag="ACGE36_002153"
     CDS             1285..3930
                     /locus_tag="ACGE36_002153"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFF8384521.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4240..5553
                     /locus_tag="ACGE36_002154"
     CDS             4240..5553
                     /locus_tag="ACGE36_002154"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8384522.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5543..6196
                     /locus_tag="ACGE36_002155"
     CDS             5543..6196
                     /locus_tag="ACGE36_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8384523.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6251..6943
                     /gene="radC"
                     /locus_tag="ACGE36_002156"
     CDS             6251..6943
                     /gene="radC"
                     /locus_tag="ACGE36_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFF8384524.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7093..7293
                     /locus_tag="ACGE36_002157"
     CDS             7093..7293
                     /locus_tag="ACGE36_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8384525.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2442 a         1259 c         1621 g         2077 t
ORIGIN      
        1 aagttacttc tggaacaccg tgtatcagag gttgcgaaaa agctgccctg catcaagtaa
       61 taagaacagc caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc
      121 agatgcaagc ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa
      181 ccttgctccc gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt
      241 cgttatgcga gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata
      301 tttacgctac ttgaagatgc tggatgactc aggtataagt agaatcaaat caaatgagtt
      361 cagcgaaatc acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga
      421 gctaggcaga agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat
      481 ccttaatact aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc
      541 attgaaacac aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa
      601 tgatccagca ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt
      661 cgcggagata gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata
      721 ttcacaatct gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc
      781 gcctgacaga gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga
      841 gttgcagacg cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt
      901 gagtaggctt attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact
      961 aaatgaagtg gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga
     1021 agcataacgc tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg
     1081 ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag
     1141 tagagataga cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc
     1201 aatttaggtg gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt
     1261 tttagtttaa aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat
     1321 ccgcaagaag ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg
     1381 agcggagata aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga
     1441 aaacttcatt tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag
     1501 agaatgcaag gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca
     1561 caagcaaaag tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt
     1621 gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa
     1681 cagtgggcta aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat
     1741 ggattatctg aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat
     1801 cgtggtgaat atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa
     1861 gttattcata aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga
     1921 tcaggcgtag tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt
     1981 gctgttcatc cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct
     2041 ttagtcaata aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca
     2101 ggtgtagtaa aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat
     2161 gatcttccac gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa
     2221 tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc
     2281 ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt
     2341 gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa
     2401 aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat
     2461 caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg
     2521 tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg
     2581 gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt
     2641 agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat
     2701 aagcgatact tccctactag cactttagta accggttatg acatcatttt cttctgggta
     2761 agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg
     2821 attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga
     2881 atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg
     2941 aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg
     3001 aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg
     3061 accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact
     3121 cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa
     3181 gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg
     3241 agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta
     3301 gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa
     3361 gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt
     3421 gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg
     3481 attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg
     3541 ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat
     3601 atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat
     3661 ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc
     3721 aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta
     3781 aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta
     3841 gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa
     3901 cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta
     3961 gctattacta aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct
     4021 tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact
     4081 cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga
     4141 taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt
     4201 tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata
     4261 gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct
     4321 ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca
     4381 aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca
     4441 gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc
     4501 aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa
     4561 caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg
     4621 gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc
     4681 ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga
     4741 cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga
     4801 atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc
     4861 atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa
     4921 ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata
     4981 ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga
     5041 gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa
     5101 gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac
     5161 gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag
     5221 aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact
     5281 aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg
     5341 acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag
     5401 agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca
     5461 aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta
     5521 ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt
     5581 gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg
     5641 ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg
     5701 ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga
     5761 tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg
     5821 tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag
     5881 taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga
     5941 gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt
     6001 agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa
     6061 tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc
     6121 accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc
     6181 tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg
     6241 agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga
     6301 acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact
     6361 gcgtaccggt caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt
     6421 tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat
     6481 aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac
     6541 ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc agcaattgat
     6601 tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa
     6661 tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc
     6721 tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa
     6781 tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca
     6841 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
     6901 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
     6961 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
     7021 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
     7081 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
     7141 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
     7201 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
     7261 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
     7321 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
     7381 ttctcttgtg gcaggtatt
//
LOCUS       DAXWLH010000130         7397 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXWLH010000130 DAXWLH010000000
VERSION     DAXWLH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.132"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            69..152
                     /locus_tag="ACGE36_002158"
     CDS             69..152
                     /locus_tag="ACGE36_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8384526.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="ACGE36_002159"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="ACGE36_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8384527.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1184..1444
                     /locus_tag="ACGE36_002160"
                     /pseudo
     CDS             1184..1444
                     /locus_tag="ACGE36_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1607..2272
                     /locus_tag="ACGE36_002161"
                     /pseudo
     CDS             1607..2272
                     /locus_tag="ACGE36_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2308..2553
                     /locus_tag="ACGE36_002162"
     CDS             2308..2553
                     /locus_tag="ACGE36_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8384528.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2656..3525
                     /locus_tag="ACGE36_002163"
     CDS             2656..3525
                     /locus_tag="ACGE36_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8384529.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3506..4240
                     /locus_tag="ACGE36_002164"
     CDS             3506..4240
                     /locus_tag="ACGE36_002164"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8384530.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4273..5181
                     /locus_tag="ACGE36_002165"
     CDS             4273..5181
                     /locus_tag="ACGE36_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8384531.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5178..5720
                     /gene="sat4"
                     /locus_tag="ACGE36_002166"
     CDS             5178..5720
                     /gene="sat4"
                     /locus_tag="ACGE36_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8384532.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5813..6607
                     /locus_tag="ACGE36_002167"
                     /note="aph(3')-IIIa"
     CDS             5813..6607
                     /locus_tag="ACGE36_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8384533.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6883..>7397
                     /locus_tag="ACGE36_002168"
     CDS             6883..>7397
                     /locus_tag="ACGE36_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8384534.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2592 a         1117 c         1554 g         2134 t
ORIGIN      
        1 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1081 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1141 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1201 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1261 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1321 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1381 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1441 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1501 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1561 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1621 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1681 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1741 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1801 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1861 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1921 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1981 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2041 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2101 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2161 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2221 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2281 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2341 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2401 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     2461 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     2521 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     2581 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     2641 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     2701 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     2761 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     2821 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     2881 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     2941 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3001 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3061 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3121 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3181 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3241 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3301 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3361 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3421 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     3481 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     3541 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     3601 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     3661 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     3721 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     3781 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     3841 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     3901 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     3961 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4021 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4081 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4141 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4201 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4261 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4321 caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct
     4381 aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga accgtttata
     4441 tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa gccggaggat
     4501 atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt tgatgattac
     4561 aataaaattg atcttacctt attgcccttg gaagagttag ataattacct aaagggcgat
     4621 aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat agttccgact
     4681 gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg ctgcaatgaa
     4741 ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat tttatttgct
     4801 attgatcatt ttaatcagat tgttcgccat gagctgctga gaatgatatc atggaaggtc
     4861 ggcatcgaaa caggctttaa attaagtgta ggcaagaact ataagtttat tgaaaggtat
     4921 atatccgagg atttgtggga gaaacttttg tccacctacc ggatggattc ctatgaaaac
     4981 atatgggaag cattatttct atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg
     5041 gaaaggcttc attatgccta tccggagtat gataggaata taacaaaata taccagggac
     5101 atgtataaaa aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata
     5161 gaagagaggc gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata
     5221 aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt
     5281 ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg
     5341 atgaagagga tgaggaggca gattgccttg aatatattga caatactgat aagataatat
     5401 atctttacta ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc
     5461 ggtacgctta tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca
     5521 gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc
     5581 ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa
     5641 tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt
     5701 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
     5761 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
     5821 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
     5881 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
     5941 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
     6001 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
     6061 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
     6121 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
     6181 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgat tagccgaatt
     6241 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
     6301 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
     6361 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
     6421 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
     6481 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
     6541 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
     6601 gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta
     6661 atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa
     6721 gttcgctaga tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag
     6781 tatggctttt ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca
     6841 taggatatac tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga
     6901 gtaacctcta ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca
     6961 cagcgattta atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc
     7021 ggaataccaa ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt
     7081 ccgccggtaa tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta
     7141 aattgccatg gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa
     7201 tctattttga agcatggtga aaaaaactat gcacaggagt tggagaacag tattaacgtg
     7261 tacagcacat ctggacagaa aaatacattg gcggataacg ttattgcagc aattcagact
     7321 gcaatctgca ataaaagggt aatatcaatt caatatcctg catcaggagg gcaagaaccg
     7381 gaaagcagaa tgataga
//
LOCUS       DAXWLH010000131         7306 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXWLH010000131 DAXWLH010000000
VERSION     DAXWLH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.133"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1037
                     /locus_tag="ACGE36_002169"
     CDS             <1..1037
                     /locus_tag="ACGE36_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8384535.1"
                     /translation="GLVIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTV
                     GFGDRAPSDIRYTSFKLFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFEN
                     GYLAQDFAVTQKTDQQEQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDG
                     EGGGYHVWSEGNGIGGLLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTH
                     YELNDDGSIEFIDMDLWQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAV
                     MDPSVPLDSPTNNEVGSEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQE
                     YEEAYKAAAQ"
     gene            1111..2214
                     /locus_tag="ACGE36_002170"
     CDS             1111..2214
                     /locus_tag="ACGE36_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8384536.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            2217..3554
                     /locus_tag="ACGE36_002171"
     CDS             2217..3554
                     /locus_tag="ACGE36_002171"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8384537.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            3541..4419
                     /locus_tag="ACGE36_002172"
     CDS             3541..4419
                     /locus_tag="ACGE36_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFF8384538.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            4416..5339
                     /locus_tag="ACGE36_002173"
     CDS             4416..5339
                     /locus_tag="ACGE36_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFF8384539.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            5336..5746
                     /locus_tag="ACGE36_002174"
     CDS             5336..5746
                     /locus_tag="ACGE36_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8384540.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            5750..>7306
                     /locus_tag="ACGE36_002175"
     CDS             5750..>7306
                     /locus_tag="ACGE36_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFF8384541.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGK"
BASE COUNT         2384 a         1215 c         1633 g         2074 t
ORIGIN      
        1 ctggcttagt cattcgaaaa gactggttgg ataatctagg actagatgtt ccaactacca
       61 tggatgagct ttatgaagta gctaaacaat ttacagaaaa cgatcctgat ggaaatggtg
      121 tggacgacac agttggtttt ggcgatcgtg cgcctagtga tattcgttat accagcttta
      181 aacttttcac ttcttatttt ggagcaccaa atggctgggc tgtagacgat aatggcaaat
      241 tcactccaga atttgataca gaagagtata tgaaagcttt gaatttttca agagatctat
      301 ttgagaatgg ctatttggca caagactttg cagttaccca aaagactgat cagcaagaac
      361 aatttgcaca aggaagaact ggaatctaca ctggaatggt ggatatcaag aatctgaaga
      421 cgatgggtca aggtattcaa gatgatatgg aattagtgcc tgtaaacaag atctcagatg
      481 gtgaaggcgg cggttatcat gtttggtcag aaggaaatgg tattggcggc ttgctagcat
      541 ttcccaaatc tcaagtaaaa gacgaagcac aattgaagaa attacttcaa tttgttgatg
      601 atcttttaga agaagatgtg tataaattga tgactggcgg tatcgaagga acgcattatg
      661 aattgaatga tgatggttct atcgaattta tcgatatgga tctttggcaa caagaggtgc
      721 aaccgttatc aagctctcgt cctagtgaag tgacgttctc tttcaaagat acagaccctg
      781 aaaaagaatt ctctaatcaa ttgatcaaag aaaatgaaga atttgctgta atggatcctt
      841 ctgtgccatt ggattcaccg acgaacaatg aagtaggatc agaaattgaa aaaatcgtca
      901 ttgatgctac ggtgcaatac atcatgggac agcttgacga agatggattc aaaaaagctg
      961 tagaagactg gaaagcacaa ggaggcgatc gaatcacaca agagtacgag gaagcataca
     1021 aagcagcggc acagtaatat agacgattgg gacaaatact tcttggtatt tgtcccaaat
     1081 cctattttaa tttttaaagg agagcaaata atggaaaact ggtcaaagaa attagcaaaa
     1141 agtatcatgg aaagaacacc tcgcttatat gaagaaaagt ggtataaagg aaaatggtct
     1201 tatgactatg gcgtcgtact taaagggttc caattacttt gggaacgaac ccaagaaaaa
     1261 atttattttg atttcataaa agacaatatc gactatttcg tgcaagaaga tggaacgatc
     1321 cgtggctaca gcgttgaaga atacaatatc gaccatgtaa atacaggaaa gcttttcttt
     1381 ttattgtaca aagaaacagg cgaagaaaaa tataaaaaag cagcagaact cctttcccag
     1441 cagttggcaa accatccacg cacatccgaa ggagcttttt ggcataaaga aatttatcct
     1501 tatcaaattt ggttggatgg cttatatatg ggttctcctt tttatgctga atttatccag
     1561 aattttagtg aagaaaaaga ttaccaagat gttttgagac agtttgaaat cagttataaa
     1621 catctattcg atccaaaaac aaatttgctg attcatgcat gggatgaaaa gaaggtccaa
     1681 ccatgggctg atccaaaaac aggtctatcc catcattttt ggagccgatc gattggctgg
     1741 tatttgatgg ctgcagcaga tacctatgaa ttacttcatg aagaaacaga ttgttctttg
     1801 ctgaaagaaa ttctagaaaa gacactggct gcattacttc catatcaaga agaaaacagc
     1861 gggacatggt atcaagtaac aacagaaaca aaacggaaag gaaattatct ggaagcttcc
     1921 ggcagtagta tgtttgttta cgcaatggct aaaggaatac gtttgggact gctgaacaaa
     1981 gaagaatggc ttccacaagt gaagaaatca catcaaggat tacttgatga atttgttctg
     2041 gaaacaaaag aaggctggct gaacttgaat aaaaactgtc aagtagcagg gttaggcgga
     2101 gcagatcagc gagatggttc atatgcttac tacatcagtg aaccaattat ttgtaatgat
     2161 caaaaaggcg ttggtgcatt cttgcaagca ttgattgaag tggaggcatt ataaatatgt
     2221 atgatatttt gacatttggc gcatcaattg atgaattgaa tactgaagca atacaacaag
     2281 ccattgatgc tgcagcaagt gacggaggcg ggaccgtagt tgttccagca ggtgaatttc
     2341 taacaggagc tttattttta aaaagcaatg tcgaacttca tttaagcgca ggagcggttc
     2401 tgaaattctc tgacgatcca aaagattatc ctgttgttca ttcacgttgg gaaggagtcc
     2461 atcgaaaagt ttatgcttcc tgtatctatg ctcaaaatgt agagaacatc tctgtcactg
     2521 gtttcggtac attagatgga aatggaaaaa aatggtggca tacttttaga aatgaaccag
     2581 ataatctcgc ttatcctcgt cctaaattga tgagtttcca taactgtcat cgaattactg
     2641 tgaaagatat taaattgatc cagtcaccaa gttggacgat caacccgatt ctttgcagta
     2701 atgcaacgtt tgataacttg acgatcttga acccagcgga ttcaccaaat acagacggaa
     2761 ttgatccaga atcatgcaaa aatgtgcgta ttagtaattg ccatattgac gtgggagatg
     2821 actgtattgc aatcaaggca gggactgaag atacgtacga aaggatcgct tgcgaaaata
     2881 tcacgatcac aaattgcacg atggtccatg gacatggcgg tgtcgtatta ggaagtgaaa
     2941 tgagtggtag tattcgtaat attaccattt caaattgtat cttccaagaa actgatcgag
     3001 gaatacgatt gaaatctaga cgaggtcgag gcgggatcgt tgaagatatt cgagtatcca
     3061 atatagtcat ggataatgtg atgtgtccat ttatactgaa tctttattat ttctgtggtc
     3121 cgcgagggaa agaaccgtat gtatgggaaa aaaaggctta tccgatagat gaacggacac
     3181 cagcattcag aaggatccat ttttcgaata tcacggcaag gaatgtccat gcttctgcag
     3241 gttttattta tggattagcg gaacaattca tacaagaaat cacatttgac aatatcgatg
     3301 tctcgatggc ccaagatgca gttcccggtg ttcctgctat gatgacagga atagaggaaa
     3361 tgagcagaca aggattttat attggctttg cgggtaaggt ttcctttaac cagatgtcga
     3421 ttgaaaatca cgagggacca gcatttctat tagaaaatac taaagaaatc agtattgaga
     3481 aattccaatc aaatcaatca gaaaatatgg atgaactggt tgttcgtaaa aactttgctt
     3541 atgcagttga atgatttttt aggtgctcca tctagcagac atacccctag tggaaaacga
     3601 ttataccgag aaaaacaaaa cggttttatg cttgaatact atcaattgta cttaaatgaa
     3661 ttggaaacag taccagccct ctatgcttat ccagaaaaag aaggaccgtt tccgccggtc
     3721 ctttacctgc attctcacgg cggagatttt tcagtcggga aaagtgaatt gatccatggt
     3781 gctccttact tagcttctcc atcttttgca gaagtactga caggaatggg atatgctgtt
     3841 tggtcgattg atgcttgggg atttgaagaa cgcggaggta tttctgaaag cgagttgttc
     3901 aaacaatttt tattaacagg aaaaacgtta tggggaatgc gtatttatga tgtgatcagc
     3961 ttgatcgatt atttggagac aagggaagac acagatactc aacggctcgc aacgattggt
     4021 ctttctatgg gaggcttatt aagctggtgg gtgacagcat tggattctcg tgtcaaagtt
     4081 tgtattgatc tagcggcaca ggtggacatc gaaatacttt tgctacatcg ccgcctagat
     4141 catcatgggt tttattacta tgtgccaaat ctattgacag cttatacaac gttagcaatc
     4201 caagaaaaaa tcgctccgcg ccctcgtctt agcttagttg gaaaaaacga tacgatgtgc
     4261 ttggacgaag gcgtactgaa gctgagaaaa gggctggaca aaaagtatca atcaatgggt
     4321 agtccagaaa acttttcttc tttttcttta acaggcggtc atatggaaac acaagaaatg
     4381 agaaatattt ggaaacaatt cataagggag catttatgag gatgaaagac aaactgataa
     4441 ttggaaagaa tgctatagat accccgacgg attttgaaac tatccaagaa ggaatcgatt
     4501 atttggcagg cctgccggat agccatccaa aggtcttgat agtaaaagaa ggaagatatg
     4561 atgaatatat agaaagcaga ttatccaatt ttaaacttgt gggagcagga acagtagtta
     4621 tctccggtaa tcgttttgca aaacaggagt atcttgacgg tagccagtta gggactttcc
     4681 gaagtgctac ttttttctta gaaggatcga atgtcgtatt agaaaatctt cacatcgaaa
     4741 atacagcagg tccaggagaa aaggtaggac aggctgttgc tttattcaat tcaggagatc
     4801 gcgtatcttt aaaaaactgt cgactatctg gttatcagga taccttatgc acgggtccgt
     4861 tgcctgattt gcaaaaagat ggtactcctt tttgtacacc ggcttctgcc aatcctgagt
     4921 attgtcgtca gacctatgaa tattgctgga ttgaaggaac ggtggatttc atatttggtg
     4981 gggcagatgc cgtttttcat cagtgtcata tccatagctt agccagtaaa aatcctggat
     5041 acatcacagc agcatctacg ccgaaaaatc aaaaaacagg gtatcgtttt gaaagctgta
     5101 ccttgactgc agaacctgat actagaaacg tctatttagg aagaccgtgg cgtccgtatg
     5161 caaatgtctt atttgaagcg tgtaagatag gtgggcatgt tcacccaact ggttgggata
     5221 attgggatga caagaaaaat gaacagactg tcaaattctc tgaacgtaat aacaattatg
     5281 aaacatcgat caaaagacct gaatggatac atttagaaaa gcagggggtt cagaaatgaa
     5341 aagaaaatgg gaactattgc taggaatggt cggtggcagt ctatctttga ttttctttgg
     5401 cgggttagca gttactttaa gtaatatgtc tgcgtcagag tttaaaaaaa gttatcagtc
     5461 gcttgctgta gatcattcaa ctttgtcttt agaaaataca tttggattat tacaggacat
     5521 gactggtctg tttgccgttg tgttgtttat ttcacttgct tttttagcag tagcattgtt
     5581 tttgactgct aaaggaaaat acttaacaac tgccactggt ttgtatttta taacaggatt
     5641 catattgttg attggcactc agtttattgc gtttcctttt gcattttttt atttcgcagc
     5701 tggggcattc agtttatatc gagtaagaat gagaaaagga gcctgaatca tgcagactat
     5761 caaagtgaaa aatcctattt taagaggatt caatccagat cctaatatat taagagtgaa
     5821 agagcggtat tacattattg tctcctcttt tgaatggctt ccaggaatcc gagtatatga
     5881 gtcgaaaaat ttagtcaact ggtcacatac aacggatatt ttgaccactc aagtatcttt
     5941 gcaaggaaat cctacaaatg gaagtatatg ggcgccacag atcagttatg ctgatggatt
     6001 gttttatctt gtttacacag atgtcaaaag tgcaggccgt ccgtttaaag atgtccacaa
     6061 ttatttgatc actgcatctg agatcacagg tccttggtcc gaaccaattt atttaaatag
     6121 taacggcttt gatccttctt tgttccatga tcttgaaacg ggaaaaaaat ggcttgtcaa
     6181 tgaactatgg gattatcgtc tggacacacc gaataaatcc agcggtatcg tattacaaga
     6241 atatgatgca gagaagaaga agcttgatgg tcccatctat aaaatatttg caggtacaga
     6301 attagcgaaa acggaggcac cacatttgta ttttcgtaac gggtattatt atttgatgac
     6361 ggcagaaggc ggtacaggaa gaggacattc agttacagtt tgccgagcaa agaatattac
     6421 tggtccttac gagcttgatc ctggttttcc tatgctgaca gctagtgaca aacctgcttc
     6481 tacattgcaa tgtacaggac atgggagctt agtacaaacc ccgtcaggaa gatggtacat
     6541 gacatatctt tgtaccagac cattaaacgg tgcggctatt ttgggaagag aaaccgctat
     6601 tcaagaagtg tattggacgt ccgacgattg gttaagactt aaagatagcc agacaactcc
     6661 gatggaagaa ataatgattg agacaaatga agaggtacaa caagaaaaag atcatcagtt
     6721 tatggataca tttgaaggag agctaaagaa gaactggaat gccagacgca ttttgccaaa
     6781 tgctgattgg tgtgatacaa aatgtagacc aggtttttta cgtctgattt ccggtgaatc
     6841 tcttcaatct accttcgaac atcatttgtt agctattcgg caaacggatt ttacttttga
     6901 tgcggagaca gcatttgaat acatgcctaa taattttaat cagatggctg gactggtact
     6961 gtacttaaat gaatccaatt atatttattg ttatgttact tggaatgagg acaagggaaa
     7021 atgcctacgt atgatagaat cagaaaacgg agtagcacaa atagaagatt ggacgattcc
     7081 actcacagaa aagaagactt ttttgaaagt cactgtaaag aaagagacag cacagtttca
     7141 tttgaaagac gtatcaactg aagaatggaa gaaagtaggt ccgcaaaaaa acatgtgtat
     7201 actctcagga gggtttactg gaaattttat cgggatagca gtgcatgatc taaataaaaa
     7261 aagaggaagt tatgctgatt ttgagttttt caagtatgat ggaaaa
//
LOCUS       DAXWLH010000132         7140 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXWLH010000132 DAXWLH010000000
VERSION     DAXWLH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.134"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     regulatory      155..329
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            467..2059
                     /locus_tag="ACGE36_002176"
     CDS             467..2059
                     /locus_tag="ACGE36_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8384542.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2072..2812
                     /locus_tag="ACGE36_002177"
     CDS             2072..2812
                     /locus_tag="ACGE36_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8384543.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            2980..4329
                     /locus_tag="ACGE36_002178"
     CDS             2980..4329
                     /locus_tag="ACGE36_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8384544.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(4457..5047)
                     /locus_tag="ACGE36_002179"
     CDS             complement(4457..5047)
                     /locus_tag="ACGE36_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8384545.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            5194..6546
                     /gene="brnQ"
                     /locus_tag="ACGE36_002180"
     CDS             5194..6546
                     /gene="brnQ"
                     /locus_tag="ACGE36_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8384546.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         2138 a         1316 c         1442 g         2244 t
ORIGIN      
        1 atagatgatt cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag
       61 tattttttca aaaaacagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt
      121 tttgtataaa taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca
      181 ttaaccctgt ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt
      241 gcactttttt gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag
      301 cagtgatgct aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg
      361 gcctttttat atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt
      421 tcatctagac cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat
      481 ggtttcagtt gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc
      541 aaatgcagat gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc
      601 acccttcaac tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa
      661 tagttacgcc ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa
      721 aaaattagtc atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa
      781 gatcgacgcg atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt
      841 taccgatcct tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc
      901 aaaagcaaaa agtttagcag atctttctgg agcaaagatt actgctcagt taaatacatt
      961 ccactatact gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt
     1021 ctcagcaatg agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc
     1081 ggaaggtgta acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc
     1141 agatggcttc cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga
     1201 cccggatatc caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa
     1261 aattatggac caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg
     1321 catcatttct gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc
     1381 tggacttacc ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact
     1441 gattggtgtc ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa
     1501 aatcggaaat gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt
     1561 acaagcaatg gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac
     1621 gattgccgct ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg
     1681 tggggggatt tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac
     1741 ccatggccaa acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc
     1801 aacaggaaat gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt
     1861 agctgatttg ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac
     1921 atttacgatc gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg
     1981 ggcggtagaa aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa
     2041 ttcaaatttg caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca
     2101 tctaagaaaa agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa
     2161 aggtgaagtt gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg
     2221 tatcaattta ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt
     2281 agctcctaaa tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt
     2341 caacctgttc aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct
     2401 gaaacgagat aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat
     2461 ggatcgttat atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc
     2521 aattgctcgg gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc
     2581 gttagaccct gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg
     2641 cttgacaatg cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt
     2701 catttttatg gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca
     2761 tccgaaagaa gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata
     2821 gctgattggg acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa
     2881 aaggtaaatt tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tcttttaata
     2941 tggaagtaat taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg
     3001 cagcgtggac tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg
     3061 gggttgtttt taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc
     3121 tatcttatta ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta
     3181 tcaaacacca ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg
     3241 gctttcatca ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta
     3301 acagccatcg gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa
     3361 ctgattgcat taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag
     3421 ttagagtttt ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga
     3481 gcttatatga tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta
     3541 tggtctcatg gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg
     3601 gtaacctttg cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat
     3661 cctgaaaaag tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac
     3721 ttaggatctc tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac
     3781 agtccatttg tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat
     3841 ttagtcgtat taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt
     3901 atgcttcgtt ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca
     3961 catcatgtgc cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc
     4021 ttgaattacg tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt
     4081 ttcttgttta tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc
     4141 ggcaaaaaag ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt
     4201 ctttacatgg cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta
     4261 ttactaacac ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt
     4321 aagaagtaag aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta
     4381 cgaaaattgc ttcttaaatt tttgtgaaat ttcggcttat ttcttggagt taaaagcttc
     4441 tgtcccagcc tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt
     4501 tataggaaaa aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta
     4561 caaaaaagac accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt
     4621 cctttgacaa aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca
     4681 tcaagatacc aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc
     4741 aaaaagcttc agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa
     4801 agagcaaaaa gatattcgac cagaccactc ggtagttaat tcgttctgcc atctgaaata
     4861 tacgatgatg gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata
     4921 atgtatttcg gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta
     4981 atacaagaac tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact
     5041 ttgtcacgat gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta
     5101 aataaccgtc gttttatata ggaaattttc tgaaaattgt tgcatatttt tcgaaatttt
     5161 gttactttta aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac
     5221 tatcttttta taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt
     5281 cccgttcatt tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg
     5341 gttaccggta tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca
     5401 ggtgtatatg aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg
     5461 ctttacttag ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag
     5521 attggtttag caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt
     5581 attgtatttt ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat
     5641 gttggaaagt tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct
     5701 tttttgaatc cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat
     5761 gcttttttca cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca
     5821 tttgggatca tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc
     5881 gctaaagaca cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact
     5941 ttgctcgctt atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg
     6001 atcacgctgg ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca
     6061 tttatcgtta tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa
     6121 acattcacag atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc
     6181 cttccttgtt tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt
     6241 ttgatgttca tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg
     6301 tttaagcagc gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt
     6361 tttgatgggt tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt
     6421 ttatatgttg cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca
     6481 gcagttatcg gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca
     6541 gattgataaa agaggttgtg aaagagacgt tcagccttat tgccgatagg ctgtctcttg
     6601 aacactgttt attgagaggt cgtgaaaaag ccgctcagtt tcgagtatta agaaatattt
     6661 tttgaaaatg gcttctcaca tttttgcata aatttgactt aataccgaga aactgacttt
     6721 tgaacaccgt ttattgagag gtcgtgaaaa agccgcttag tttcaagtat taagaaatat
     6781 tttgtgaaaa tggcttctca catttttgca taaatttgac ttaataccga gaaactggct
     6841 tttgaacacc gtttattgag cgtctataaa ctgacctgag tattagagaa acaaaacggc
     6901 aaagtagctc catatacttt gccgttttgt tttgaactaa taccgaagag aagctgatca
     6961 aacaacattt cttttataat atagatttta gacatatata tgatataaaa atagttttcg
     7021 caaaaaaact taacaacttt cgaaaacttt cgtcacaaaa tattcacaac tagtgactat
     7081 tatataagca taccaacaaa caaccctaac aacacttttt catttttaac tccttatcct
//
LOCUS       DAXWLH010000133         7057 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXWLH010000133 DAXWLH010000000
VERSION     DAXWLH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.135"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            10..855
                     /locus_tag="ACGE36_002181"
     CDS             10..855
                     /locus_tag="ACGE36_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8384547.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1136..2356
                     /locus_tag="ACGE36_002182"
     CDS             1136..2356
                     /locus_tag="ACGE36_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8384548.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2353..5073
                     /locus_tag="ACGE36_002183"
     CDS             2353..5073
                     /locus_tag="ACGE36_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8384549.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5057..6001
                     /locus_tag="ACGE36_002184"
     CDS             5057..6001
                     /locus_tag="ACGE36_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8384550.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6070..>7057)
                     /locus_tag="ACGE36_002185"
     CDS             complement(6070..>7057)
                     /locus_tag="ACGE36_002185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8384551.1"
                     /translation="AATSALAVLTLAACSGDTNKDIATMKGGTITVSDFYDEAKLESS
                     NQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAGYTKDTYKE
                     YIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDVKKSADDGD
                     DFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDVITTTNPTS
                     YATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEELKDANVKIK
                     DDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDSSK"
BASE COUNT         2504 a         1134 c         1353 g         2066 t
ORIGIN      
        1 ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac tcttttgaat
       61 tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc ggaagactgt
      121 tttccattca tttgttctgg aagagaagta gaagacatta ataatattct caaaaaatct
      181 ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg agaaaaaaag
      241 atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa
      301 tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta taaaaataaa
      361 atgaaaaaaa tttttcacga ggtagggagt gaattttcta tcaaagagct aaactatgag
      421 cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga aattatagaa
      481 aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca attgaaagct
      541 aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc ggcacctgta
      601 aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca
      661 atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa tgacttatct
      721 atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga aatcatgaaa
      781 aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc atttgaaaga
      841 attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa tacggaaaaa
      901 tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc gaatataaca
      961 tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat aaacttattt
     1021 tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt tcggttattg
     1081 atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat ttttaatggt
     1141 gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac tgacaactct
     1201 tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat caaatatcat
     1261 tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata attttcatca
     1321 aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac ggttagaaaa
     1381 gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa aagagcggta
     1441 ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag agacaaaaaa
     1501 aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc gtcaaccgtg
     1561 gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt accatattgg
     1621 gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt tccaaccctc
     1681 aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg tgccaacggt
     1741 cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga
     1801 acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc aaatacatcc
     1861 gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga aagcaagaac
     1921 gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg atggatttag
     1981 tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt tggaacggat
     2041 acaatcagga gagctattag actatttgtt agaggaattc tcagatagga taaatgattt
     2101 ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc cacttgctgc
     2161 ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag atttccaaca
     2221 aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg aactgccgga
     2281 atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt ttttatttga
     2341 ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa atggcgtcaa
     2401 aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga agcaggaaaa
     2461 tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa
     2521 aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg gcttaaacat
     2581 cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag
     2641 gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat cgcacctttg
     2701 acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat ggaactagat
     2761 catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac gggcagttct
     2821 caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt caagaaaaaa
     2881 ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca agaaaaaatt
     2941 catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga acaggaatac
     3001 gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca attttcatta
     3061 gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct caaaaaaaac
     3121 agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt ctccaaacgg
     3181 tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa gcatagcggg
     3241 atggatcagc agtcagccag atattatttt tatttagagc aggagcagga actaaaagat
     3301 ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag attacaaatt
     3361 gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca ttggcagaaa
     3421 aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct ggctgaagaa
     3481 agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc taaagaacag
     3541 caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc attattacaa
     3601 aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat ggctggcgga
     3661 gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct tttactaatt
     3721 ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa aactaaaaat
     3781 gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga tctagcctta
     3841 gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa acagattctt
     3901 cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga tacgctcaaa
     3961 ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg cgtatttgag
     4021 cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt
     4081 gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa aatggtattg
     4141 ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta tcagcaaaat
     4201 actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg gatacaagaa
     4261 tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc tgtcttttta
     4321 caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc ggaaatgctt
     4381 actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact gcgccaactg
     4441 aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga
     4501 ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact ttatcaacaa
     4561 gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc acttgaaatc
     4621 gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt accccagttg
     4681 ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca gaagatatta
     4741 ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat ctatgagtta
     4801 tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct ctcattagaa
     4861 gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta tgatcatcag
     4921 cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca aataatttgt
     4981 ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa
     5041 ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc agtttgaaca
     5101 atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa aatttattgc
     5161 ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg cttcggagga
     5221 ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa gagaggttta
     5281 tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag atgagccagg
     5341 agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat
     5401 caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc gctatctact
     5461 taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc atcatgcatt
     5521 tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat caatcgccga
     5581 tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc atgatttagg
     5641 aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag gaaatttgat
     5701 cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag
     5761 cgaagctgac gaagatgttc tcgtattacg tcatatggtt ttagcacacc atggcttatt
     5821 agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac atcaaatcga
     5881 caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa
     5941 atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa aagatttata
     6001 gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct tcgctttttt
     6061 tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt ttgttgaatc
     6121 agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt ctttcgtact
     6181 tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt tgatttttac
     6241 atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt cacttagttt
     6301 agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat tgcctttatt
     6361 ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag gatttgttgt
     6421 agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt ttacttctgc
     6481 aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt ttgtctctgt
     6541 atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg ctgatttttt
     6601 cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta cttctggatg
     6661 gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc
     6721 agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg tatatccagc
     6781 agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt cgtattctgc
     6841 atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt tataaatgat
     6901 catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat agaaatcaga
     6961 tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc
     7021 tgctagagtt aaaacagcca acgcactagt agctgcc
//
LOCUS       DAXWLH010000134         6902 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXWLH010000134 DAXWLH010000000
VERSION     DAXWLH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.136"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            5..472
                     /locus_tag="ACGE36_002186"
     CDS             5..472
                     /locus_tag="ACGE36_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFF8384552.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            479..2971
                     /locus_tag="ACGE36_002187"
     CDS             479..2971
                     /locus_tag="ACGE36_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFF8384553.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            3192..4115
                     /locus_tag="ACGE36_002188"
     CDS             3192..4115
                     /locus_tag="ACGE36_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFF8384554.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            4121..5374
                     /locus_tag="ACGE36_002189"
     CDS             4121..5374
                     /locus_tag="ACGE36_002189"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFF8384555.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            5508..6854
                     /gene="gor"
                     /locus_tag="ACGE36_002190"
     CDS             5508..6854
                     /gene="gor"
                     /locus_tag="ACGE36_002190"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFF8384556.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         2386 a         1127 c         1528 g         1861 t
ORIGIN      
        1 taagatggca catcagaata cctcggattt aattgaatca tatataaaga aaattctgga
       61 aaatagtgaa atgatcgaga ttcgaaggat tgaaatggca gacctcttta actgtgttcc
      121 ttcacaaatc aactatgtga ttaatacccg ttttaccatc caacgtggtt atctagtaga
      181 aagcaaacgt ggtggtggtg gttatatacg aattgcaaaa gtaaggattt cagataaaaa
      241 acaattactt gagcagataa atcagttatt tgatgataca atcagtgaga aaaatgcttt
      301 tgctattatt caaaaacttt atgaagacca aataattaca aaaaaagaag gaaacttgat
      361 gttgagtgca attgcaaaaa acacattgaa tttcaatgaa tatgaagatc atacacgtgc
      421 tcggattctc cgagcatttc tggaacgttt aagttatgaa gatgggaagt gagattagat
      481 ggaagaactt tttactgaaa gagcccaagc agtattagaa attgcacaag aagaagcaaa
      541 acggttgaaa catcaatcag ttagttcaga acatgtttta ctagccttag tggtcgagca
      601 aaatggaatt gctggaaaag tattacgtga aatggatttg aatgaaacgg aaatttatga
      661 agaaattgaa catctgattg gttatggtgg tatccgttct taccctaaag gagtctttct
      721 tccatattca ccaagaatga aacaagtttt tgctttagct agcagcgaag tgaagaaaac
      781 tggttctcaa aaagttggaa cagaacatat tttgatgagc ttattaaaag atgaaagtat
      841 catggctact cgaatcatga tcaacttagg tatcagcctt tcaaaagccc gtcaagttct
      901 gaaacaaaaa atggggctag ctggtgattc tgctggaaaa ggaatgaatc gtcgtagaaa
      961 tgtaaatgta caagataaac ggcaaacacc gcaaggaaca cctacgctag attcattagc
     1021 acgtgatttg acgaaattag caaaagaaaa caagctggac ccagttgttg gtcgttcgag
     1081 agaagtgaaa cggcttatcc agattcttag ccgacggaca aagaataacc ctgtactggt
     1141 gggtgaacct ggtgtaggca aaacagccat tgctgaaggg ttagctcaaa aaatcatttt
     1201 aggtgaagtg ccagaagaca tgcaagaaaa gcgcctgatg atgcttgata tgggatcttt
     1261 ggttgctggt acgaaatatc gcggtgaatt tgaagatcga atgaaaaagg tcatcgacga
     1321 aatctataat gatggtcaag taatcttatt cattgatgaa ttgcatacct taattggtgc
     1381 aggtggagca gaaggagcaa ttgatgcttc gaatatcttg aaaccggcgt tagctcgagg
     1441 agaattacaa acgatcggag caacgaccct agatgaatat caaaaatata tcgaaaaaga
     1501 ttctgcatta gagcgacgtt ttgctcgcat tcaagtagat gagccaactc ctgaagaagc
     1561 agaagtgatt cttcaaggat tacgtacaag atatgaagag caccatggag tggagatcac
     1621 agatgaagct gtacgagcag cagtcaatct ttctgttcgt tatatcactt ctcgtcaact
     1681 acccgataaa gcaattgatt tgatcgatga atcagcagcg aaagtccgtt tggatcaaac
     1741 cgatcactta acaaaatcaa ctgtgatcaa actagaaatc gatgaactag tacaagaaaa
     1801 ggaagcagcg atccaaaaac aagactttga aaatgctgct caattacgca gacaagaaaa
     1861 agcactacgt aaaaagctgc aaaaagtttc tgcaattgaa gcaaaacagg agcaaggtta
     1921 ttctgatcgt gtaacagaag aagatgttgc tacagttgta tcggagtgga ctggtgtccc
     1981 tctacagcag ttggagaaaa aagaaagcga acgcttgtta gaactggaag gacttctgca
     2041 tgaacgtgtc gttggtcaag atgaggctgt caaagcagtt tcacgtgcga tccgccgtgc
     2101 acgaagcgga ttgaaagatc ctgatcgtcc aattggttca ttcatgttcc ttggaccaac
     2161 tggtgtcgga aaaacagaat tggccaaagc actttcagaa gtcatgtttg gaagtgaaga
     2221 tgccctgatc cgtgttgata tgtcagaatt tatggaaaaa tacagtacaa gccgattaat
     2281 cgggtctcct ccaggatacg tagggtatga tgaaggtgga cagttgacag aaaaaattcg
     2341 tcaaaaacca tattcagtga ttctcttaga tgaagtggag aaagcacatc cagacgtttt
     2401 caatttactt ctgcaagtat tggatgatgg tcatttaaca gattctaaag gtagaaaagt
     2461 ggactttaga aatacaatca tgattatgac ttcaaatatc ggagcgacac aaattcgaga
     2521 agaaaaaaat gtcggattca atgtccaaga tgttactaaa gatcacaaag ccatgcaaaa
     2581 acgtatctta gaagagttaa agaaagcttt ccgacctgaa ttcttgaatc gaatcgatga
     2641 gactgtcgtc ttccattcat tgagtaaaga tgaaatccat acaatcgtcc aaatcatgag
     2701 caaagcgatt atcaaacgat tgaaagaaca agatattcaa gtgaaaatca caccatctgc
     2761 aatcgatgtg atcgggaaag ctggatttga tcctgaatat ggtgcacgtc caatccgtcg
     2821 cgcacttcaa aaagaaattg aagatcgatt gagcgaggct ttgcttggcg gagaaattcg
     2881 tttaggtgac cacgtaacag taggtgcttc aaaaggtaag atcacattga atgttcgcgg
     2941 acctaaaaag gaaacagtag gttccctata gttacttggc ctgtgtagct gaatttttat
     3001 aatagtaaga cttgattaag aggttgtgac aagatttttg tcacaatctc tttttatatt
     3061 tggacagaat atatcgaact ttattttgat tttttgtgaa ataattttca aaaatccgac
     3121 tttaaataga agaagtattg gaaagttttt agagctatgc tactattatg aagttgtagg
     3181 aggagaaaat tatgatcgaa ctcataacca cagtagaaac tattgaacaa gcagaacagt
     3241 tgcttgctgc aggtgcagat acactatatt tcggtgaaga gcaatttgga ttgcgtctgc
     3301 ctgcttcatt tacacgagaa gaacagcgac aattggtaga attagcacac aagtatggta
     3361 aaaaagcgaa tattgctgta aatgggatca ttcatcctga aaaaatgaaa aatgtacctg
     3421 aatatttggc ttttttaaaa gaaataaacg tagatgtgat cacagttggt gatacaggta
     3481 ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc gtatatttat gatgctgaaa
     3541 cattagtaac gagcgcacgt caaatcaatt tctgggcgaa aaaaggcgca gttggagcag
     3601 tacttgctcg tgaagttcct tttgaagaga tgaaggcgat ggaagaaaaa ctcgatattc
     3661 ctgtagaaac ccttgtgtat ggtgctacat gcatccatca atccaaacgt ccactgctac
     3721 agaattacta taactatacg aaacaagacg aacaaaaaga tcgtgaaaga ggtcttttta
     3781 tctcggaacc taaaaaagaa gagacacatt attctattta tgaagatagt catggcacac
     3841 atatttttgc cagcaatgat ctgaatctct cgaatgaatt gaatgaactt tatcagcatc
     3901 attactgtac atggaaattg gacggattat ttactccagg cgaaaaattc gtaaacatca
     3961 ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg tagctgggat caagaaaaag
     4021 cggaaaaaat gactagcgaa atcagtaaat tgcatcctga aaaccgtgaa ttagatacag
     4081 gattctttta tattgatcct tctgctatca aataaggagt atgatgaaag aaatgacagt
     4141 aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca ggaacactgg aaaagttaaa
     4201 aaccgcgatc cattatggag cagatgcagt atatattggc gggaatgcat atggtttaag
     4261 aagccgtgca ggtaatttta cttatgaaga aatggctgaa ggagttgctt ttgcaaaaga
     4321 acatgatgcg aaggtatacg ttgctgctaa tatggtcacc catgaaggaa atgaagatgg
     4381 agcaggggaa tttttccgca gtttgcgtga tgtcggtatt tctgcagtga ttgtttctga
     4441 tccagctttg atcgaaattt gtgcgacaga agccccaggt ctgccgatcc atttatccac
     4501 tcaagcttca gcaactaatt ttgagacatt ggaattctgg aaagaagaag ggttggaacg
     4561 tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca gagattcgta aaaatacgga
     4621 taccgaaatc gaagcattta tccatggtgc catgtgtatc tcttattctg gtcgatgcac
     4681 attgtccaac catatgtcga tgcgggatgc aaaccgtggc ggatgttctc aatcttgtcg
     4741 ctggaagtat ggattgttcg atatgccttt tggtcaagaa cgaaacagtt taactgatac
     4801 aggcgaagtg gaagaagaat tctcgatgag tgcagtggat atgtcaatga tcgaacatat
     4861 tccagaattg attgaaaacg gcgtagacag cttcaagatc gaaggaagaa tgaaatcgat
     4921 ccattatgta tctacggtgt caaatgtcta taaagcggct gtagacagtt atatggaaga
     4981 tcctgaaaac tatgtctgca agcaagaatg gatcgacgag ttatggaaag tagcacaacg
     5041 agaattagct acaggttttt actacaatac accaactgaa aacgaacaat tattcggtga
     5101 acgtcgtaaa atcccagtat ataaatttgt cggtgaagta ttatcttatg atgaggcaac
     5161 acagattgct acgatacgtc agagaaactt gttttctgta ggagatgaaa tcgaatttta
     5221 tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt atgcataatg aagaaggcga
     5281 atccattgac cgagcaccaa atccaatgat gattttaact atgccagtcg cacagccggt
     5341 gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa agctgatata tccctgtttt
     5401 aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact tgttttctgt agaaaaagga
     5461 tcgttaaata aaggttttga ttgatctctt caataaggag gaacaaaatg caaatatatg
     5521 attacatcgt aattggtggc ggctctggcg gtattgcttc agcaaacaga gcagggatgc
     5581 atggcgcaaa agttttgctg attgaagcga atgaattggg cggaacatgt gtcaacgtcg
     5641 gttgtgtacc taaaaaagtt atgtggcaag caagtacgat tttggaaaca attgaacgag
     5701 atgcctcaag ttacggtatc caagcagatt taaaaaatgt tgatttcgcc ggtctagtag
     5761 aaagacgaga aaaatatatt gattttctac acggtgctta tcaaagagga ttagatagca
     5821 atcatgtcga ggctataaaa ggttacgcta cttttatcga tagtcaaacg atcgaagtaa
     5881 acggtgaaac ctatcgtgct cctaaaatct tgatcgctac tggaggccgt gcaagtaaaa
     5941 tgaccattcc tggtggagaa tacgcaattg attcaaatgg attttttgaa ttaacagagt
     6001 tacctaagag agcaatcgtt ttaggtgctg gatacattgc ggctgaactt tcaggtgtat
     6061 ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa agaacgacca cttagaacgt
     6121 ttgataaaat gctttcggac aatttgatcc aaatgtacca agaagctgga ataaaaactt
     6181 acgcacatca tattgctaaa gaaattacta aaaacaatga cgaatatacg gtgctatttg
     6241 aaaatggtga aaccttaact ggtgattgtg tactttttgc cggtggacgt gtaccgaata
     6301 ccgaaaaatt aggtctggaa aatacaaatg tggaattgga caaaaaaggg tttatcaaag
     6361 tagataaata ccaaaataca acggatgaac atatttttgc tgttggtgat gtgatcggta
     6421 aattagatct gactcctgtt gcaatcgcag ctggtagacg tttatcagaa cgtttattta
     6481 atggaaagaa agattcttat cttgactata aacttgttcc gacagtcgtc tttacgcatc
     6541 caccaattgc aacaattggt ttgacagaag aagaagcatt ggaaaaatat ggcgaaaatg
     6601 aattgaaagt ttatcgttca cgctttaccc caatgtactt tgcattaaat gattatcgcc
     6661 aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga aagaatcgtc ggcttacatg
     6721 ctattggtgt tggtgtcgat gagatgctgc aaggttttgc cgtggctatc aagatgggtg
     6781 caactaaaga agactttgac aatacagtag ccatccatcc aacaggagcg gaagaattcg
     6841 taacgatgcg ataatttaat attaacagtt ccaacagctt tttattgaac aaaccatgta
     6901 aa
//
LOCUS       DAXWLH010000135         6853 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXWLH010000135 DAXWLH010000000
VERSION     DAXWLH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.137"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            208..2250
                     /locus_tag="ACGE36_002191"
     CDS             208..2250
                     /locus_tag="ACGE36_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384557.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACGE36_002192"
     CDS             2261..2881
                     /locus_tag="ACGE36_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFF8384558.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACGE36_002193"
     CDS             2893..3351
                     /locus_tag="ACGE36_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384559.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACGE36_002194"
     CDS             3370..3648
                     /locus_tag="ACGE36_002194"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8384560.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACGE36_002195"
     CDS             3673..5040
                     /locus_tag="ACGE36_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8384561.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE36_002196"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE36_002196"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFF8384562.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="ACGE36_002197"
     CDS             6227..6718
                     /locus_tag="ACGE36_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384563.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
BASE COUNT         2163 a         1119 c         1493 g         2078 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg att
//
LOCUS       DAXWLH010000136         6706 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXWLH010000136 DAXWLH010000000
VERSION     DAXWLH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.138"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            262..528
                     /locus_tag="ACGE36_002198"
     CDS             262..528
                     /locus_tag="ACGE36_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFF8384564.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            528..2255
                     /gene="ptsP"
                     /locus_tag="ACGE36_002199"
     CDS             528..2255
                     /gene="ptsP"
                     /locus_tag="ACGE36_002199"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFF8384565.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            2419..3465
                     /locus_tag="ACGE36_002200"
     CDS             2419..3465
                     /locus_tag="ACGE36_002200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8384566.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            3482..4705
                     /locus_tag="ACGE36_002201"
     CDS             3482..4705
                     /locus_tag="ACGE36_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8384567.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            4794..5681
                     /locus_tag="ACGE36_002202"
     CDS             4794..5681
                     /locus_tag="ACGE36_002202"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8384568.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            5767..5997
                     /locus_tag="ACGE36_002203"
     CDS             5767..5997
                     /locus_tag="ACGE36_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFF8384569.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(6054..>6706)
                     /locus_tag="ACGE36_002204"
     CDS             complement(6054..>6706)
                     /locus_tag="ACGE36_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HFF8384570.1"
                     /translation="KGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISL
                     LALRAQLPDLQEMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQK
                     DIELVTYFIRRIQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVS
                     PDFYYEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         2304 a         1119 c         1422 g         1861 t
ORIGIN      
        1 gaatgataaa cgtcaggaga agagaaagcg acaagaaaaa gaaaataaac acgtttgtta
       61 taaaatgtga agaaaaggat gaaaaagaat ccggaataat ttgaatactt ttatctgaaa
      121 ttgaatgaaa actgttgtat ctaacgaaaa aaaatgttaa tctttacaga tgaaagggtg
      181 gcatttgtta ggattctctg aattactaaa aaagaaaacc ctcacataaa aacaacactc
      241 acttaaagga gaccgattaa tatggaaaag aaagaatttc acgtagtagc agaaactggg
      301 atccacgctc gtccagctac actattagta caaacagcaa gcaaatttaa ctctgatgta
      361 aacttagagt ataaaggtaa atcagtaaac ttgaaatcaa tcatgggcgt tatgtcttta
      421 ggtgtaggtc aaggttctga tgttactatt actgcagaag gtgctgacga agctgatgca
      481 atggcagcaa tcgttgaaac aatgaagaaa gaaggcttat ctgaataatg gttgaaatgc
      541 taaaagggat tgccgctagt gacggtgtag ccgttgcaaa agcttacctg ctagttcaac
      601 cggatttaac attcagtaag gcaacagtag aagatactgc ggctgaagaa gctcgtttgg
      661 atgctgcatt agctaaatct actgaagaat tgcagcaaat tcgtgaaaaa gcagcgcaaa
      721 gcttaggtga agcagaagcg caagtatttg atgcccattt gatggttctt tcagatccag
      781 aaatggtagg tcaaatcaag caaaatatta aagacaacag cgtaaatgct gaatcagcgc
      841 taaaagaagt aacggatatg tatatcggta tgttcgaagc aatggaagat aatgcttata
      901 tgcaagagcg tgctgcagat attcgtgatg ttgctaaacg tattttagcg cacttattag
      961 gcgttacttt accaaatcca tcaatgatca acgaagaagt ggtcgttgtt gcacatgact
     1021 tgactccaag tgatacagca caattagatc gcaattttgt aaaagcgttt gtaacagaca
     1081 ttggtggacg tacttcacat tctgctatca tggcacgttc tttagaaatt ccagcaatcg
     1141 ttggtacaaa agaaattact gctaaagtca aagaaggcga tattttggct gtcaacggga
     1201 tcgaaggcga tgtaatcatc gatcctacag atgaacaaaa agctgaattt gaaaaagcag
     1261 gtgcagatta cgcagcacaa aaagcagaat gggaaaaatt aaaaaatgca gaaactgtca
     1321 cagctgacgg caagcatttt gaattagctg caaacatcgg aacaccaaaa gatctagttg
     1381 gtgtccataa taatggtgga gaagcagttg gtctgtaccg tactgaattc ctgtatatgg
     1441 attctccaga cttcccaaca gaagatgacc aatatgaagc ttacaaagct gtattagaag
     1501 gtatggaagg aaaacctgta gttgttcgta caatggacat cggtggagat aaagaactac
     1561 catatctgca attacctcat gaaatgaatc cattcttagg atatcgtgca ttgcgtatca
     1621 gtttatctga acaaggcgac gaaatgttcc gtacacaaat gcgtgcattg cttcgtgcat
     1681 ctgttcatgg taacttacgt atcatgttcc ctatggtagc tacactcaaa gaattccgcg
     1741 cagcaaaagc gatctttgaa gaagaaaaac aaaaattgat cagtgaaggc aaggaagtat
     1801 ctgatacgat ccaagtcggc atcatgatcg agattccagc agcagcagtc ttagcagaca
     1861 agtttgcaaa agaagtagat ttcttctctg taggtacgaa cgatttgatt caatacacaa
     1921 tggcagcaga ccgtatgaac gaacgcgttt cttacttgta ccaaccatat aacccatcaa
     1981 ttttacgttt gatcaaaaat gtaattgatg cagcacacgc tgaaggaaaa tgggcaggta
     2041 tgtgtggaga aatggctgga gatcaaacag ctgttccatt gctagtaggt atgggtctag
     2101 atgagttctc aatgagtgcg acatctattc ttaaaacgcg tagtttaatg aaacgcttgg
     2161 acacagcgaa aatggctgaa cttgctgatc gtgcattgaa agaatgcgat acgatggaag
     2221 aagtcgttga acttgttcat gaatatgtaa aataaacaac gtatcaaaag ctggtgtggc
     2281 ttgtaaaaga gctacgccag tttttttgtt cttttttaag aaaacgtttt tgtcagtaag
     2341 aatagataaa aatagggctt aagtttgagt gacagtttgt aggctatgtt atactatata
     2401 agtgaagggg gacgtaaggt gaaggtatta ttatattttg aaagtgaaaa gatgcttgca
     2461 aaatctggaa tcgggcgtgc gcttgatcat caaaaacgtg ctctgacgga agtcggtatc
     2521 tcttatacac tagatgaaaa agaagattat gacatcctgc acatcaatac atacggaatc
     2581 aatagtcata atatggtcaa taaagccaga agagaaggaa aaaaagttgt ctatcatgcg
     2641 cattctacag aggaagattt ccgaaattcc tttatcggat cgaaccagct ttctcctatc
     2701 gtaaaaaaat atctggtagg attgtaccaa aaagcggact atctgatcac gccaacacct
     2761 tattcaaaac agttgttgga aagctatagt atccgtgtgc cgattcaagc tatttccaat
     2821 gggatcgact tagagaaata tcgccctgat ccaatgaaag aacaaaagtt cagagaatat
     2881 ttcaaactgt ctccggatca aaaagtaatc atttgtgtcg gtttgttttt cgaacgtaaa
     2941 ggaatcattg atttcgtaga aattgctaag aagatgcccg aatacacatt tatttggttt
     3001 ggtcatgttc ccatgtactc gattcctcgg aacatccgga aaattgtaaa agaagatcat
     3061 cctgaaaatg tcctgtttcc tggttacatt cgtggagaga tcatagaagg tgcgtattca
     3121 ggtgcagatc tattcttttt cccttcttat gaagagacag aagggatcgt tgttttagaa
     3181 gctttagcaa gcaaacaaaa cgtacttgtt cgagatattc cagtgtataa tggctggtta
     3241 gaagatagca agaactgcta tatggggaag acaaacgaag aatttatccg actgatccaa
     3301 cagattacca atcaagaact accgagtacg acacaagccg gttatcaaac agcaaaagag
     3361 cgaagtatca ataaaatcgg tgaagaactt aaaaatgtat acgaaagtgt actgaatgaa
     3421 aaagtctcta gcaaattaaa aaaagtaaat caggtcaagg actagtttgg aggcgaaaaa
     3481 catgaaaatc ggctttttta ccgataccta ctttccgcaa gtcagcggag ttgctacatc
     3541 gatcaaaaca ttaaaacaag aattagaaag acatgggcat gaagtctata tttttacaac
     3601 gaccgatcct aatgcggata agttggaaaa agatgtgatt cgaatgccaa gtgtaccatt
     3661 tatttctttc aaggatcgaa gagttgttgt tagaggcatg tggtatgctt atttggttgc
     3721 caaagaacta gaactagatc ttattcatac ccatacagaa ttcggagcag ggatacttgg
     3781 gaagatggta gctaagaagt tgaaaatacc tctgatccat acttaccata cgatgtatga
     3841 agactacctt cattatatcg gtaaaggaaa agtgatcagg caatcccatg taaaatactt
     3901 gtctagatta tttgcaaatc atacgacagg tgtagtttgt ccaagtgaac gtgttattga
     3961 tacattacgt agctatggtg taatagcacc gcttcgcgtg attcctactg gtatcgacat
     4021 tgaaaaattc aaacgttctg atatcactca gcaggatatc agcaaattaa gggagtcact
     4081 aggtcttcag gaaaaccatt tgatgattct ttctttaagc aggatttctt atgaaaaaaa
     4141 tatccaagcc ttgattaatg ggtttcctca ggtcatctcc gcttatccta acgccagatt
     4201 agttatcgta ggaaaaggac cttatgtaga agagttagag gaattgatct cagaactgca
     4261 gttagaagaa tttgtgcagt tcacaggaga agtggacaac aatgatgtag cactttatta
     4321 taaagcggca gattattttg tcagtgcttc tacttcagaa acacaagggc ttacttatac
     4381 agaagcgatg gcagccggaa caccttgtgt cgtggaagga aatgcttatt tgaaccgatt
     4441 gttcgatcat ccttcattag ggaaaacttt tgaaaccgac gaagactttg caccagcact
     4501 tattgattat atttccagtg aagttcctaa agatccaaaa gttttagaag ctaaacttta
     4561 tgatatttca tctacacatt tcggcgaagc tatgatcgaa ttttatcaag acatggttgt
     4621 ttatcatgaa gaagagatgc gaagaaaaga agaagaagac tcgattgaac gaatcaaaat
     4681 aaaattaaat tcatttaaaa gataaaattc aaacattttt tatgaaaaaa tccttgtttc
     4741 tagcgtatac tagcatagaa gcaaggattt ttgattttta ggaggtcgaa aaaatgaaac
     4801 ttggttttat cggaacaggc gtcatgggaa gtgctgtcgc acgccatcta ctagaagcag
     4861 gacacgaagt agctgtctat aatcggacaa aagcaaaagc tgatccttta gtaacagaag
     4921 gagctatctg ggcagataca ccaaaagcgg tagcagaaca aagtaatatt ctctttacaa
     4981 tggtaggtta ccccaaagat gtagaagaaa tctactatgg acagtcgggg attttttctg
     5041 cagatatctc aggacatatt ttagttgact tgactacaag tacaccaagc ctggcagaaa
     5101 aaattgcaaa aacagcaaaa gaaaaaggcg cagatgcgtt agatgcaccg gtatccggtg
     5161 gtgacttagg agcaaagaat ggtacactga cgatcatggt cggtggagaa gaagccgttt
     5221 atgatcaagt actgccttta ttcaaagaat ttggtacgac atttacattg catggttctg
     5281 ctgggaaagg tcagcataca aaaatggcta atcagatcat gattgcaggt acgatgacag
     5341 gaatgacgga aatgcttgtc tatgcacaaa aaaacggact tgatttaaag aaagtgattg
     5401 agacgctttc aggtggaagt gcagctaact ggtctttaag caattattct cctcgtattt
     5461 taaaagaaga ttacacacct ggtttttttg tcaaacattt tatcaaagat ttaaagattg
     5521 cattagaaga agcagaaaaa atggaccttg tattgccagc aactacccaa gcattgaaac
     5581 tatatgaaga gttggcagat aaaggttttg agaatgacgg aacccaagct ttgatcaaac
     5641 tttggtggcc tgaaggaaaa ataccagaaa aaaaatcata aaaaacgagt ttttttagag
     5701 aaaatcaatg caagtaggct atgcttttga tacaatataa cagaaataag aaaaggagga
     5761 cctccgatga cacattcaca attagtcgca atcataaaac gactggaggc tatggtcgag
     5821 tcagcagata atgaactgca agttcgccgt tttgaaaaag aaggcgtaga gaagtgtaca
     5881 gtaacttatg ataaagcgac cgatacattt gaattaactg aaacagatac tcatcagcaa
     5941 tacgaatttg ataatatcga tatcgtagcg atggaaatct atgacttaat ccaataaatt
     6001 ttagaataat tgacatttga agttgaagat gcctaaaaga agccgagaag caactaattt
     6061 agttgctttt cagcttcttt tttgtttaac agttcaattt cagactgttc tttttctaat
     6121 tttttgcggt aactgatcaa gatttgtaca ggatcgattt cttcttttga caattgacgt
     6181 ttcaaagata aatgagcttc tttcttttca taatagaagt caggagaaac gtcagcagag
     6241 gcaatcattg tttctgcaag ttgaccataa caaacgaggt agtgcttttt ctgactctca
     6301 tctgctgcta atggcatgct gaaggtgagc gatggtgttc ttaaaaaatg cttttgttcc
     6361 tggatccgtc taataaaata agtaactaat tcgatatctt tttgaagcat accggctttt
     6421 tcgaaattca actgatagat gaaatgttgt tgtctttttt tcaagtaagt tagtagcttt
     6481 ttattttggc cttgaaacag cccttggagt tccttttttt tctgttcaaa agtcatttcc
     6541 tgtaggtcag gaagctgggc tcgcaaagct aacaaactaa tttcagacaa ccaaggcatt
     6601 tgataagttt cttccattat acgcaaaata gacggcattt ttttatagag acgaaaaggc
     6661 ccgtatgttc gagccgtagg agtatctgtt aatacaaatc ccttat
//
LOCUS       DAXWLH010000137         6303 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXWLH010000137 DAXWLH010000000
VERSION     DAXWLH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.140"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            334..714
                     /locus_tag="ACGE36_002205"
     CDS             334..714
                     /locus_tag="ACGE36_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFF8384571.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACGE36_002206"
     CDS             696..1610
                     /locus_tag="ACGE36_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8384572.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACGE36_002207"
     CDS             1855..2544
                     /locus_tag="ACGE36_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384573.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACGE36_002208"
     CDS             complement(2665..2751)
                     /locus_tag="ACGE36_002208"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8384574.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACGE36_002209"
     CDS             3427..4377
                     /locus_tag="ACGE36_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8384575.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACGE36_002210"
     CDS             4398..4931
                     /locus_tag="ACGE36_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8384576.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACGE36_002211"
     CDS             4947..5117
                     /locus_tag="ACGE36_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384577.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACGE36_002212"
     CDS             5567..6100
                     /locus_tag="ACGE36_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384578.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACGE36_002213"
     CDS             6112..>6303
                     /locus_tag="ACGE36_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384579.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2324 a         1098 c         1175 g         1706 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaata agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcctc tgtatcggtt gatacaaggg ctacttggca tagtgtacat tatgttaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAXWLH010000138         6235 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXWLH010000138 DAXWLH010000000
VERSION     DAXWLH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.141"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..278)
                     /locus_tag="ACGE36_002214"
     CDS             complement(<1..278)
                     /locus_tag="ACGE36_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8384580.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKI"
     gene            complement(414..593)
                     /locus_tag="ACGE36_002215"
     CDS             complement(414..593)
                     /locus_tag="ACGE36_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384581.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(590..940)
                     /locus_tag="ACGE36_002216"
     CDS             complement(590..940)
                     /locus_tag="ACGE36_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384582.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(933..2258)
                     /locus_tag="ACGE36_002217"
     CDS             complement(933..2258)
                     /locus_tag="ACGE36_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8384583.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2708..3322
                     /locus_tag="ACGE36_002218"
     CDS             2708..3322
                     /locus_tag="ACGE36_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8384584.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3487..3729)
                     /locus_tag="ACGE36_002219"
                     /pseudo
     CDS             complement(3487..3729)
                     /locus_tag="ACGE36_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3933..5252)
                     /locus_tag="ACGE36_002220"
     CDS             complement(3933..5252)
                     /locus_tag="ACGE36_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFF8384585.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(5386..5994)
                     /locus_tag="ACGE36_002221"
     CDS             complement(5386..5994)
                     /locus_tag="ACGE36_002221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384586.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
BASE COUNT         1920 a         1165 c         1014 g         2136 t
ORIGIN      
        1 tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc
       61 ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa
      121 gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg
      181 taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca
      241 ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt
      301 tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt
      361 gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata
      421 tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa
      481 gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct
      541 gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc
      601 gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt
      661 cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg
      721 gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga
      781 ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt
      841 gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc
      901 ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg
      961 cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa
     1021 gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct
     1081 aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg
     1141 gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac
     1201 ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc
     1261 ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg
     1321 acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct
     1381 tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt
     1441 ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca
     1501 ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga
     1561 ttccagttag caagatctct aatgctcata atccccatct gattgagtct ttttttcatt
     1621 cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg
     1681 ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct
     1741 agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct
     1801 ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca
     1861 gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc
     1921 agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg
     1981 atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag
     2041 tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct
     2101 aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat
     2161 ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa
     2221 aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac
     2281 attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa
     2341 gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca
     2401 ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc
     2461 aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc
     2521 cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc
     2581 ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg
     2641 aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt
     2701 aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca
     2761 gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc
     2821 aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat
     2881 ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt
     2941 caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga
     3001 agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc
     3061 catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat
     3121 cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga
     3181 tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat
     3241 tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga
     3301 cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt
     3361 tcctacatat aattactatc tataaaatta agtcttactc agaacagata aagcataaaa
     3421 ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta
     3481 tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt
     3541 attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat
     3601 tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg
     3661 agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat
     3721 aaagctatcc ctgaataatt catagcttta attctttggc acattttatt gaaatatgta
     3781 tcactaatat ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca
     3841 aaaatattga ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa
     3901 cttcccagta aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg
     3961 cgagcaaaga ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag
     4021 actcgtctac ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa
     4081 tgaatggtca ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag
     4141 ttgtaagcta tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct
     4201 gttttgtcaa agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt
     4261 ttggcataga tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata
     4321 aattcatgtc gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga
     4381 ctttgtgacc aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc
     4441 cacttttgat tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta
     4501 ttcttcagtc gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt
     4561 gtagcgaacc cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag
     4621 tgctctaata aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa
     4681 cgtagttcag cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa
     4741 ccataggttt ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa
     4801 tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg
     4861 ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa
     4921 aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca
     4981 ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata
     5041 atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg
     5101 tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg
     5161 acgagacccg aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg
     5221 aattttactt ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt
     5281 tagtcacttt aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga
     5341 aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt
     5401 attcaggcta tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca
     5461 ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg
     5521 tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta
     5581 ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat
     5641 tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt
     5701 aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat
     5761 aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga
     5821 agttatatct ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat
     5881 ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc
     5941 aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc
     6001 aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc
     6061 aataaattct atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt
     6121 gttccctgag tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct
     6181 cctccaatat ataaaaatta cttaactgta tccaaattta atatgtaaat aaatc
//
LOCUS       DAXWLH010000139         6169 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXWLH010000139 DAXWLH010000000
VERSION     DAXWLH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.142"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(32..310)
                     /locus_tag="ACGE36_002222"
     CDS             complement(32..310)
                     /locus_tag="ACGE36_002222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384587.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1042)
                     /locus_tag="ACGE36_002223"
     CDS             complement(425..1042)
                     /locus_tag="ACGE36_002223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384588.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="ACGE36_002224"
     CDS             complement(1437..2261)
                     /locus_tag="ACGE36_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8384589.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="ACGE36_002225"
     CDS             2604..3020
                     /locus_tag="ACGE36_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384590.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ACGE36_002226"
     CDS             3017..3487
                     /locus_tag="ACGE36_002226"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8384591.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ACGE36_002227"
     CDS             3501..4274
                     /locus_tag="ACGE36_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8384592.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ACGE36_002228"
     CDS             4288..5100
                     /locus_tag="ACGE36_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8384593.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ACGE36_002229"
     CDS             5154..6119
                     /locus_tag="ACGE36_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8384594.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
BASE COUNT         1947 a         1026 c         1198 g         1998 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgtt
//
LOCUS       DAXWLH010000140         6166 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXWLH010000140 DAXWLH010000000
VERSION     DAXWLH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.143"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            273..522
                     /locus_tag="ACGE36_002230"
                     /pseudo
     CDS             273..522
                     /locus_tag="ACGE36_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(766..1596)
                     /locus_tag="ACGE36_002231"
     CDS             complement(766..1596)
                     /locus_tag="ACGE36_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFF8384595.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            1804..3138
                     /locus_tag="ACGE36_002232"
     CDS             1804..3138
                     /locus_tag="ACGE36_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8384596.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGISDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3179..4090
                     /locus_tag="ACGE36_002233"
     CDS             3179..4090
                     /locus_tag="ACGE36_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8384597.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLTPKWVYYSGGDGK"
     gene            4087..4941
                     /locus_tag="ACGE36_002234"
     CDS             4087..4941
                     /locus_tag="ACGE36_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8384598.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            4938..5555
                     /locus_tag="ACGE36_002235"
     CDS             4938..5555
                     /locus_tag="ACGE36_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8384599.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            5557..6075
                     /locus_tag="ACGE36_002236"
     CDS             5557..6075
                     /locus_tag="ACGE36_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8384600.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1832 a         1102 c         1235 g         1997 t
ORIGIN      
        1 tttcataggt tcttgttttt atacggcttt atttcttgga cttcttgaag cattttccat
       61 ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag atctttttca tttcattttt
      121 ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt tttagattta ttgtctttag
      181 acctaattga gcgcgccgag cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa
      241 taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa ataccacatt gtattcacac
      301 caaagtatag aagaaaaata atttataatc aatatcgagc caatattcag gagatcatca
      361 agagattgtg caaatctaaa atataaattt gggtatcgac acttttgggt ggaaggatac
      421 tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa agtacattca agaacaagaa
      481 aaacatgata gtgcgttaga taagctgagt gtacgagagt aagacgatcc ttttaagggc
      541 taaggagtag tacataaacc ttttaagagg tgggcaaaac tggcaaaggc aatatggctt
      601 gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc ggtgatttga gcttatagct
      661 ctagagaaaa ccaccctttt gaagggtggt tatgattaat gactttagtc agttttgctc
      721 acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta gatatttatt tcttgaatgg
      781 aggtgtccca tacatttttg gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt
      841 aggttctttc aaccattgat attcgccttc gccatccagt gctttgccac tagcccaaga
      901 accttcacta ctttggtctc cctctgagaa gttatagaag tcataagcaa tgtcttttcg
      961 ttctttgtca cgcgggaaag tacttgggac taagattgaa ccttcttttt cttctaattc
     1021 tttcactgca cattcccata gattttgatg atagctatct cttgcaagga gaacagacag
     1081 catatctttt acgccacgat catcgatcat ttcgtaaata cgtgctactt gaagtcttcc
     1141 ttgtgattcg gcatttaaat tagctctaaa gtctgctaac aagttgccac tcgcaatgat
     1201 ataggcaccg ctccatggat tgcccacgct gtcagcagga cgaggtccta agccagctac
     1261 gatcgcgtgc tgtggattca ttccagagat aatggcaccg attgttggat ctttttgcat
     1321 cgctgcttct tgatcttcaa caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa
     1381 catttccacg tggccgattt cttctgttgc aatgtccatc agcatatctt tatatttttc
     1441 ttctcctcgg cagctccagc cttggaacag gtactgcata gctacactca tctcgccata
     1501 ttggccgccg atcagctcct gcagatattt cgcgattaat ggattcggac gatctggttt
     1561 tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa attcctccta atctattttt
     1621 aatacattct cattctgcta cgaaagatta aaaaattcaa agaaagcgct cgcgaattat
     1681 ataaagtatt ggtatcatta ataatttttt tagaatcata ccttataaat tgttattttc
     1741 agaacaaaat gatatcgtta tattaaaacc ggcatcggtt acattaaaat ttggaggtaa
     1801 aatatgaaaa aattattgtt tacagggttg ctggcaagtt cagcattgtt tttattagga
     1861 ggatgcggat caagtaatgc aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa
     1921 acaagtgatg gaagaaaaaa tattgatttc tggtcctttt ggggatccgg tgcacgaaaa
     1981 gaagctatcg aagaaatcat tgaggatttc aatcaatcac aaaacgagat ccatgtaaat
     2041 tataagtatc agccatgggg tgacatctgg actaaatctt tatctgcaat aacagctgga
     2101 aatgcacccg atgttattgt acaggacata aattccgttg cccaaagagc agaagcacag
     2161 caagcaacta atctctccaa atatgtagaa aaagacactt ctaaggattt ttatccgcag
     2221 ctttgggata cagtagaata tgaaggtgat ccttatgcga tcccattcaa tacagataca
     2281 caagtcattt tttataataa aaagttattt aaagaagctg gaatcagtga acaagatcta
     2341 ccgacaactt gggatgaatt agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa
     2401 gaatttacac gcatcggttt ttatccatta tggaatttag gggctgatgt atgggcgcta
     2461 aacgcagata acgggactag ttggtttgac aaagatgaaa aagtcaagat tgatactaaa
     2521 aataaagtgg aagccttgga gtggatccaa gactggcaag attattatgg gaaaaatacg
     2581 atcaataaat tagaagccga atttgggtct ggtatatctg atccatttat ttccggatta
     2641 gtagccatgc gtgcccaaaa tatcaattat tactctagtt tgatggaaaa tgcaccggat
     2701 gattttgatt ttggcgtgat gcagctccca gaaaaagaag aaggttcagg ccattggtcg
     2761 tggggtggcg gattcgtctt ggaagtacca tacggagcaa aagatcctga ggcctcttat
     2821 gaattcatca aatatctttc ttctccagaa gtacaagaaa aattcggcga aaaaagtttt
     2881 gatatcatgg cgagcaagac agccaatgag aatttagtga ataatgacaa cctaacggat
     2941 aaaggaaaaa tgatctatca aatggcggac gaaaacttta aaaatacggt gatcacacca
     3001 gttcctttag atgctccgga ttacacgaca ttagtgaatg aacagattga ccagattctt
     3061 ttaggaacaa aatcaccaaa agaaggattg gcagatgcac aaaaagcagt agaaaactta
     3121 gtcaagcaaa atcaataatc catttttgac tgtctaggta cagaggaggc aagaaaattt
     3181 gaagcgttta tctatccgaa agaaaaaaga agcaagatgg gcctatttat ttatctcgcc
     3241 ttttattatc gggttcttgg tatttatgct agggcctatg cttttttcag ttattggtag
     3301 tttcaccgat tataatttga catccaagat gaatttcatt ggtctagcaa atttcaaaag
     3361 aatgttcgtt cacgatgatt tattttggaa atcgttatac aacaccgttt atttcgtcat
     3421 cttcaatgta ccgctgacga cgattttcag tgtgttttta gccacagtac tcaatcaaaa
     3481 aatcaaagga atcagtgtat tccgtacagc tttctattta ccggcagtct tatcaggagt
     3541 agcagtctac atcttatgga tgcagctgct ttctccatct gcaggattga tcaacacatt
     3601 tctgggctgg ttccatatcc ctggaccagc ttggctgttt gatccacaat ggacgaaacc
     3661 tgcgttgatt ttgatgaaaa tatggagttc tggaggagca atgctacttt acttagctac
     3721 attacaaaat gttcctagat ctttatatga atcagcagaa atcgatggag caggcgtttg
     3781 gggaaagttc aaaagtatta ctttgccatt aatcactcct attatttttt acgatgtgat
     3841 cacctctacg atcggttctt tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg
     3901 aacaggcgga cctgcaaatt ctttgctttt ttataatctg catatgtgga acaaggcgtt
     3961 cgtagctttt gatatgggtt atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt
     4021 ggttctgacg tttgtcaatt tgaagcttac acctaagtgg gtctattatt ctggaggtga
     4081 cggcaaatga tggattattt caaaaagacc agtgtgaatc ggcaaacaaa aaaaatacta
     4141 agttatgtcc tcatgacagt aataggaatc attttgatca ttccattgct ttggatggtg
     4201 tttacttcat tgaaaccaat ggaagaaatc gttcgttatc ctccgacctt tttcccagaa
     4261 aaaatcgtat gggaaaacta tctggatacg attgccgcct ttcctttttg gcgctatgca
     4321 agaaatacat tattcatcac tgtgctagta gtgatcggta atgtcctttc aaattctttt
     4381 attgcttatg gatttgcgaa gcttgatttt cctgggaaaa agctgatgtt tgctttggtt
     4441 ctttctacga tgatgatccc ggggtttgta acgatgatcc ctcaatatgt attattctcc
     4501 aagatcggct gggtaggtac ttatttgccc ctgatcgttc catcgttctt cggcaatgcg
     4561 ttcaatatat ttctgatgcg tcaattttac ttatcgatca ataatgaatt gatcgaagct
     4621 gctgaaatcg acggagcgaa tcatttgtat atttggagtc atttgatgct tccattgacg
     4681 aaacctgcct tgattaccat tgcgatcaat tccttcaacg cagcttggaa tgatttctta
     4741 ggtcctctgt tatatatcca agatcaagaa aaatacacgt tacagattgg cttgcaagtt
     4801 tttcagaacc aagcaacgac acaatggaat tatctgatgg caggagcaac acttgtcttg
     4861 atcccgacga ttttgctgtt cttttttgca caacgttatt tcattgaagg gatggatttg
     4921 acaggaggtt caaaaggatg atgaatcgat cgattggtaa aaaagccaat gattatttct
     4981 tagaaatcat tgaatggatt ccacgtctta tccatttaca gctactatgg ttcctgtgcg
     5041 tcttgcctgt tttcacatta ggaactgcaa gtcgaacagt tctgtatatg atttatcatt
     5101 acaaaaaaaa tgaagagaaa aagctcggtt cactattctg gaaaaagttt cgtgagaact
     5161 tctctcttta tggaaagcaa gatagtttag caagtgttta ttttcttctt ctattgatcg
     5221 attatcagat tttccgctat tggggtgggg gaattggcta tacattgatg tatactacgt
     5281 tgtttttgct tctattaagc gcgcttctct ttagttacaa aatcttgctt caactggaac
     5341 aaaaagaagc ttcttggatt actgcatttt tcttgttttt taatgatttc aaaagcgcgc
     5401 ttttctattt ggcaggcacg ttgatccttc ttgtcgcatt ctggtttgca ggaccaatct
     5461 attttgcttt attaggtttc agtttcttgt tttattttca agtcctgctt tttatccatc
     5521 gtacgcaaga aaaggttctt aaaaaggagg aataacatgt tgcgtttgat tgctaaaatc
     5581 gatgatagta ttatcaacaa gttcgccaag caacggaaaa cggatagtcg aaaaatcaat
     5641 tttttgaaat ttatcagtaa tatctctaat ggtggtttta tttggttgct ccttgctgct
     5701 gttttagtct tgatacctga tactagagaa tatggtctag ccattcttat cggcagtttg
     5761 atctattttt ttgttggttt gctttttatc aaaaaaacga caagacggaa aagaccttat
     5821 gaaaaaaatc gtcggcttgt cctttacaca agtaaaccga agacttcttc ttttccttca
     5881 ggacatacag cgctagcctt tttatcagta gctgcgttgt tcgaattaag tccagttgca
     5941 ggaatattag cctttttatt tgcttgtttg atcgcttatt cgaggatttt tttactttta
     6001 cattatccta ctgacatttt aggaggagct attttaggaa gtgttattgg catcctatgc
     6061 gctgccgtta tctgaattaa ttgtacgaat aaagcatgag tgcgtaaatg cgcatttgtg
     6121 ctttattttt ttgtttcgag gaaagcgtag ttgtttattc atttgt
//
LOCUS       DAXWLH010000141         6140 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXWLH010000141 DAXWLH010000000
VERSION     DAXWLH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.144"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            235..924
                     /locus_tag="ACGE36_002237"
     CDS             235..924
                     /locus_tag="ACGE36_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8384601.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            911..2119
                     /locus_tag="ACGE36_002238"
     CDS             911..2119
                     /locus_tag="ACGE36_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8384602.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2413..3684
                     /gene="serS"
                     /locus_tag="ACGE36_002239"
     CDS             2413..3684
                     /gene="serS"
                     /locus_tag="ACGE36_002239"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFF8384603.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4233..5717)
                     /gene="guaB"
                     /locus_tag="ACGE36_002240"
     CDS             complement(4233..5717)
                     /gene="guaB"
                     /locus_tag="ACGE36_002240"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFF8384604.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1877 a         1162 c         1205 g         1896 t
ORIGIN      
        1 aacatttcta gcttacatga tcattggtta gttctggtcc tttttcgttc gtctttataa
       61 ctccgagaga cgtaaaatcc ttttgttttc agtttaatgt tcatgttctt gtcttattca
      121 caccaactag atgtctcacc ttctaaaatt ttccttaaca aagtgctgct tttatctatt
      181 tctgcttttt tttgctagaa taatagtaac aatatgactc tggaggattt atttatgaaa
      241 attttgattg ctgacgatga taaagaaatc gttgaattat taagtatcta tgttcacaat
      301 gaggggtatg aagcagtaaa ggcttatgat gggaaagaag cattatctaa aatcagaaca
      361 atccctgata ttgaattact tattttagat atcatgatgc ctgagatgga tgggatgcaa
      421 gtagtgaaag agctacgaaa agaatcccaa ataccaatca ttatgctgac agcaaaaact
      481 actgatatgg ataagatcaa aggactggtc gctggtgcag atgattatgt gaccaagcca
      541 tttaatccgt tagaagtaat ggctagagta aagtctttat tacggcgtac aaaaatgcag
      601 attgccccag atcagcctga tgtattagaa gtaaatgcat taatcatcaa taaagactct
      661 catgaagtta aaacaatcga tgggaaagaa atccagttaa cagctttgga attcggtatc
      721 ctctacttgc ttgctagtca tcctaatcga gtatttagcg cggatgaaat ttttgagcgt
      781 gtttggaaac aagaaagtat tgtctctgct aaaactgtca tggttcacgt tagtcattta
      841 agagataaga ttgaagaagc aactggtggt gaaaaagtca ttcaaaccgt ttggggagtg
      901 ggctataaaa ttgacgcaag atagtaaacg agcaactgaa aaacgccgtc ggatcaattt
      961 gacttcaaaa gaaatcagcg aattgttagc ggaaggaatc atcacgatta tccttttgct
     1021 tctattaaat gtttctattc tggttgttat cagttcggtg atcaacagtt caccttcttt
     1081 gacgaatgcg atttgggatt caaagaatat ttttgcagaa cgattgaaca ctgatttgtt
     1141 ctggaacggc cggaatttta ttattccctt tttctttcta ttagatattg gtgttttgta
     1201 ttggcgcttg attcgaagat atcgccaaat gcaattgcgg catatcatca gcgaacttca
     1261 ctacatcgca aacggaaatt atgatcatcg tatccctttt gaactgagtg gtgacttaag
     1321 ccgagtggta acaagtatca atggattggt ggatagtacc gtagcagcga ttgaagacga
     1381 acgtaaaatc gaaaaatcaa aagatgaatt gatcacgaat gtcagtcatg atatccgtac
     1441 cccactcacg tccatcattg gatacctagg actgatagaa gatggccagt atcactcaga
     1501 agaagatctt ttaaagtata cgcatactgc ttatatcaaa gcaaagcaga tgaaatcttt
     1561 agtggacgac ttatttgaat acactaaggt acgccagcct gctgtcccgg tgaatttttc
     1621 tgcctttgac atgatccagt tgatcgaaca gttagcagct gattttgagt tggaagcttc
     1681 taaaaaaaat atccaaatac ttgttcaatc aaaagtagac tcccttatca tggatggcga
     1741 taccgaaaaa cttgtacgtg tcttcaataa tcttttgacg aatgcgttga aatacggaaa
     1801 aggagcaacg aagatcgtaa tcgaagtaga acgtatcggt tcagaagttg ttgcaacagt
     1861 caaaaataac ggagcaatga tcccacaaca ggcaatcgat aatctattcg accgttttta
     1921 tcgtgtagaa gaatctcgtt cacaagcaac tggaggaact ggacttggtt tagcaatcgc
     1981 tcaaagcatt gtggctttgc acggcggata catttatgca aaatcagata agcaatggac
     2041 ttctttcatc atgcaccttc caatcaagaa aaatgaaaaa ttacctattg atacacagga
     2101 agttattgac gaaagctgaa atattcgcta ttcttattac aattattaga ataaatgaaa
     2161 aacacagaac ccagtacact ttattctttt tcagagagct gatggcaggt gtaaatcagc
     2221 aaaggataat ttggaagttc cagttcgttt acgtgccctt tcgaaataag aaaggtcggt
     2281 tcaacagccg ttatgtgttc aacaagtgca gctgacgctg aacttgggtg gtaccgcgaa
     2341 tttcagactg attcgtccca agaataatgg gatggatcag tcttttttta ttttcaaaaa
     2401 ggaggaaatc atatgttaga tgtaaaaatg atgcgacaaa atattgaaga agtacaagaa
     2461 aaattgatga ctcggggcgt aaaaaaagaa acattagctg aattcatgga actagatgaa
     2521 aatcgtcgta agctactagt caaaagtgaa gaattaaaaa aatatcgaaa tgatgtttct
     2581 gctgagatcg cccaattgaa gcgtgcaaaa gaagatgctt ctgctaaaat cgctgaaatg
     2641 aaagaagtcg gtggcaacat caaagcattg gatactgaaa ttgctgaagt ggatgaaaaa
     2701 ttgaaacaaa ttgcaactac tttgccaaac cttccccatg attctgtgcc agttggaaca
     2761 gatgaagagg acaatgtaga aatccgtcgt tggagcgaac cacgtacatt tgcttttgaa
     2821 ccaaaaccac actgggaaat tgcagaaaat cttgatattt tagattttga acgtggcgca
     2881 aaagtagccg gtagccgttt tgtctattac aaaggattag gtgctcgatt agaacgtgct
     2941 gtctacaact tcatgcttga tcagcatatt tacgaacacg gctatactga aatgatcact
     3001 ccttatatcg tcaacagtaa agcgatgttt ggtacgggac aatttccaaa attcaaagaa
     3061 gatgttttcc aattggagaa tactgatcta acattaatcc caacagctga agtaccatta
     3121 actaactatt ataatgatga aattttagat ggtgcagatt tacctattta tttcactgca
     3181 ctaagccctg cttttcgttc tgaagccgga agtgctggtc gggatacacg aggactgatt
     3241 cgtcttcacc aattcaataa agtagaaatg gtcaaatttt cggatgtaga gcattcttac
     3301 gaagaattgg aaaaaatgac agctgatgca gaagatatct tgaaaaaatt gcatttgcct
     3361 tatcgtgtga tggccttatc aactggagac atgggctttt ctgcagctaa aacttatgac
     3421 ttagaagtat ggatacctgc acaagatact tatcgtgaaa tcagttcttg ttctaactgt
     3481 gaagatttcc aagcacgccg cgcaatgatc cgttatcgcg ataatgatgg aaaaatccac
     3541 tatactcata ctctgaatgg ttcaggactt gcagttggcc gtacagtagc tgccattttg
     3601 gaaaactacc aaaatgaaga tggttctgtc acagttccag aagcgctcgt tccttatatg
     3661 gggaatctga aagttattaa ataaagaggc tgtgaaaaag ccgcttagtt tcgagtatta
     3721 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     3781 aactggcttt tgaacaccat ttatcgagag gttgtgaaat cagcgaatta acctgagtat
     3841 tagagaaaaa aatggaaaaa tagtccctca tattttaccg tttgttgaac taataccgag
     3901 ggtcagctga ttgaacaccg tttagtggag aggtcgtaag cctacccccc agctcctagt
     3961 tacaaggaca aattcaaaaa acagctcttc atattttatg aatgttgtac ttgtgattcg
     4021 gaggtggcaa gggaacactg tttattcaaa aagaggctgg gacagaagcg tttaactcca
     4081 agaaataaga agaaattcac gaaaattgct tttcaaattt ttgtgaattt cttcttattt
     4141 ccgaaggagt tgcttctact tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4201 tctttacttt tgtcccacgc tctttaatgt ttttaagatt caactgaata gtttggtgct
     4261 tcttttgtga tttgtacatc atgagggtgt gattctttta atccactgcc gctcatttgg
     4321 ataaattgcg cttcatcacg taattgctgt agattggctg ccccaacata acccattcct
     4381 gaacgcaaac cgccgatcat ttggaaaata atatctgcaa cacttccttt ataagcaacc
     4441 cgaccttcga ttccttctgg tactaattta tttgcttcat tgacactacc ttggaagtaa
     4501 cggtcttttg aacctttttc catcgcgcct aatgagccca tacctcggta tgttttgaat
     4561 cgacgtcctt ggaagatttc gaattcacct ggtgattcat ctgtccctgc caacatactt
     4621 cctagcatca ctgcatgtcc gccggcagct aatgctttga cgatgtcacc agaatatttg
     4681 atccctccgt ctgcaataat cgctttgcca tattgacgag caactgatgc tgcatcataa
     4741 atagctgtga gctgaggtac acctacaccg gctaccacac gtgtcgtaca aatcgatcct
     4801 ggtccgatac caacttttac gacatctaca cctgcatcat ataaagcttt tgtcgcttca
     4861 gccgtcgcaa cgtttcctgc aatcaatgtt gcatctgcaa aagttgaacg gatttcttga
     4921 atttttcgga tcacacctgc actatgtcca tgagctgtat cgatgatgat tgcatctgcc
     4981 cctgcttcta ataaagcatt cgcacgctca aatgtatcgc ttgttacacc aacggctgct
     5041 gctacaagca atcgaccatg ttcatctttt gctgcatttg gaaattcgat cactttctcg
     5101 atgtctttga tcgtgatcaa tccgcttaaa cgaccttcgt tatctacgat tggtaatttt
     5161 tcgattttat gtttttgcaa aatcttttct gcatctttta gagaggtacc tactggggca
     5221 gtcactaagt tgtcttttgt catcacatca gcaatcggca tgtgataatc tgttacaaaa
     5281 cgcatgtcgc ggtttgtaat tatccctacc aattttctgt tttcaagtgt ttcaacgatt
     5341 ggcacaccac tgatacgata tttgctcatc aattcttctg cgtctgctac aagatgctgt
     5401 ggcgttaaga agaacggatc aatgattacg ccactttcag aacgtttgac ttttcttact
     5461 tcatccgctt gctggctgat cgtcatgttt ttatggatca cacctagtcc gccttgtctt
     5521 gccatagcaa tggccatctt gctgtcggta acagtatcca tacttgcact gatgattgga
     5581 atattcaact taatattttt agctaattgt acgctcatat ccacttcgtt tggtaacacg
     5641 tgactttctg ctggtatcaa taagacatca tcaaaagtaa atcctttttt cgtgaacttt
     5701 gtttcccagt tggacatttt tagtaccact cctctaattt agtttatcta ttaaaataat
     5761 tgattttttc atttaagtca atgatctact tggaatcaag ttagctttct catagaatac
     5821 caatataaag cgtttacatc tgtgtatatt aaaagagatt gtaaaaaagc tgtcctgcat
     5881 caagtaataa gaacagccaa tcaaaaacag cttctcatgt ttttgaattg gttgggttta
     5941 ttaccgcaga tgcaaacttt tgaacaccgt ttagtggaga ggctgtgaaa aagctgctta
     6001 gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga
     6061 cttaataccg agaaactggc ttttgaacac cgtttatcgt aaaaagggac tgtgacaaga
     6121 cttttgtcac agtcccttac
//
LOCUS       DAXWLH010000142         6061 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXWLH010000142 DAXWLH010000000
VERSION     DAXWLH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.145"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..615
                     /locus_tag="ACGE36_002241"
     CDS             <1..615
                     /locus_tag="ACGE36_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384605.1"
                     /translation="SKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDI
                     KESELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKE
                     KVTSADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTA
                     TATYDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            661..1605
                     /locus_tag="ACGE36_002242"
     CDS             661..1605
                     /locus_tag="ACGE36_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8384606.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1621..2400
                     /locus_tag="ACGE36_002243"
     CDS             1621..2400
                     /locus_tag="ACGE36_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFF8384607.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            2412..3110
                     /locus_tag="ACGE36_002244"
     CDS             2412..3110
                     /locus_tag="ACGE36_002244"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFF8384608.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            3138..3902
                     /locus_tag="ACGE36_002245"
     CDS             3138..3902
                     /locus_tag="ACGE36_002245"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFF8384609.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            3915..5732
                     /locus_tag="ACGE36_002246"
     CDS             3915..5732
                     /locus_tag="ACGE36_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFF8384610.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            5743..>6061
                     /locus_tag="ACGE36_002247"
     CDS             5743..>6061
                     /locus_tag="ACGE36_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid O-acetyltransferase NeuD family
                     sugar O-acyltransferase"
                     /protein_id="HFF8384611.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGI"
BASE COUNT         2124 a         1116 c         1282 g         1539 t
ORIGIN      
        1 tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa attttctaaa ccctgaaata
       61 acagaagaaa caatcacaag tttgtcaaat aagctatcct ctgtcaaact ttcagcagat
      121 gattacgaca tcaaagaatc agaattgcct aaagaagctg ttgcaatcca agatgagaag
      181 aaagcaatcg tgacacagtt agatagtgca gaatcaaaac tgaagatcca gacagctgtc
      241 aacgatttat ttactaaaaa tgtatcaaac tggcagcaag ctgtagacga tgtcgtcatc
      301 aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg aaaatatgag cttttttaaa
      361 gatagtcctt ggaaaacggt tgttatgcaa tatctaggat tcgctgatac tcagatcgcg
      421 caagtcacgc aactagatca gttattcgat acgatgttga aggatggtca agtgactgcc
      481 accgctactt acgatcaata tttgacggcg ctcagtcaaa tagagcagat ccggaatgaa
      541 aaaataagcg cagcatacgc aacaaaagca caaactgttg ctcagcaaat gggctatagc
      601 aatataagtt attgacagtt aaaacgttga ttcaagcaag gttagcaaac ggaggaaact
      661 atggcaagac gaagaagaaa atcaagaggg aaaaaaatat ttctgattat tttagcagtt
      721 cttatcgtat tagtattagc aattgtagga gtaggagcaa aattatatct ggatctctca
      781 cattctattg agaaaactta cgaatcagtg gaacgaaccc aaggagaacg aacgaaacca
      841 gttgatctgg acaaacaaga atctttctct gttctattga tgggcattga tactggagat
      901 ttaggtcggg tcgatcaagg aagatcagat acgatgatgg ttgccacagt cagtcctgaa
      961 gacaaacaga caacgatcgt cagcattcct cgagatacgt atgtcgacat cgtaggtcgt
     1021 gacacaaaag acaagatcaa ccacgcttac gcattcggcg gagctgccat ggcgatggat
     1081 accctacaaa aatatgtaga catcccaatc gatcactatg tctctatcaa tatgaaaggg
     1141 ctaaaggaac tcgttgatgc agttggtggt atcgaagtca ataatgacct cactttctca
     1201 caagatggct atgactttcc aatcggtaaa attacattga acggggatca ggcattatct
     1261 tattctcgta tgcgttatga agatccaaat ggtgactatg gtagacaaga gcgtcaaaga
     1321 aagatcgtag aaggtgtagc aaaaaaagta ttgagtttaa acggtgtcag caactatcaa
     1381 tccgttttga atgctctaga gtcgaatatg aaaacagaca tgagctttga tgaaatgaaa
     1441 aaaatcgctt tagattatcg ggaggcattt ggtacgatca agcaagatca gctgcaagga
     1501 gaaggcttca tgcaagataa tatctcctat caaaaagtca gcgatcaaga acttgatcgt
     1561 gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata aataggaaag tggagaaaaa
     1621 atggaagaga caatcagctt gaaagaaatt tttgaaacat tacgcaaaca cttggtaaca
     1681 atcatcatct caatgtttgc tggcttggcc atctcaggaa tcgtaacatt tttcatcttg
     1741 acaccaaaat acagttcaca agcacaattg atcgtgacac tgccacaatc agacacagca
     1801 aatgtcaatg atgtcaatac gaacctgcaa atgatcaata cgtataaaga tatgatcgtc
     1861 agtgatttag tactaaatga agtcaaagat cgtttagaaa cagaagacaa tgtgaaaatg
     1921 actgctggac aaatcaaaga tgcgatctcc gtcaatcagt cacaaaattc acaaatgttc
     1981 tcgatccagg caataagcac gaatgcagtg actgctcaac agatcgctaa tacaacttca
     2041 caagttttcc aagaaaacgc caaagacgtg atgaatgtcg ataaaatctc gattatttca
     2101 ggtgcagttg ccaatacgac acctgtttcg ccaaataata aattgaatct agtgattggc
     2161 ttagtgttag gaatgatggt aggcgtaggt ctggcattct tattcgaatt acttgatcgg
     2221 acagtgaaag atgaaaaata tgtgacggat acattaggat tcccaatctt aggaacagtt
     2281 ccagaaatgt cagcaaaaga attgaacgct ggtatccgta aaaaaggatc agctcctgca
     2341 cgaccaaaag ctacaaacga taaaacagcc ccatcacgca gaagccgctc aagagtctag
     2401 gaggaagaaa aatggcacga acacagaaac aaaaagcaac tcaaactcat gcagtcagtt
     2461 tgatcacatt aacaaaccca tcttcaccaa tcgccgaaca atatcgtacg atccggacga
     2521 atatccagtt tgcctccgca gctggtcagc aaatcaaaac gattgtcgtg acttcatcag
     2581 gtcctggaga aggaaaatcc accactgcag caaacattgc cgttgttttt gccaaatctg
     2641 gtcaacgggt actgctagtc gatgcagact tacgtaagcc cgtcatctat aaaacgttca
     2701 agttgaacaa cgcaagcgga ttaagtactg ttcttagttc atcaggaagt gtagcagatg
     2761 agatccaacg aacaccagtc gacaatttgt cgattctgcc aagtggacct aaaccaccaa
     2821 atccatcaga actactaagt tcgccaagaa tggatcagat tttagcagaa gcaagacaac
     2881 tatttgatgt agtgattttc gacatgccac cagtcgttgc cgtaacagat gcacagatca
     2941 tgtcttcaaa aacggatggc accttattgg tcgtacggga aaatacgtca cgaaaagaat
     3001 cattgactaa agcaaaagaa ctgttagata tggtacaagc gcgagtcctt ggtgttgtat
     3061 acaatggagc agaacacagc agagatgctg gctattacta cgggaattaa gaaataaaaa
     3121 gagaggagac aagagaaatg atcgatctac attgccatat cctgccagga atcgatgacg
     3181 gagcagaaaa catagatgcc agtatcgcca tggctgaaaa agcgattcaa caagggatca
     3241 cacatatttt atgcacaccc catcacaata atggaaaata cagcaatgaa aaatcacaag
     3301 tcatttctct agtcgcttcg ttacaagcag aattagagaa aagacaattg cctttgacgt
     3361 tattggaagg acaagaagta cgtattaccg gaacactgat tgaagacatc caccgagatg
     3421 aaattttatt tacagattta gatgatacat atcttctaat cgaatttccg acactggaag
     3481 tgccgttata tgcagaatgg ctcttttttg agttgttaca actaggaaag acaccagtga
     3541 tcgtccatcc agaacgcaac gcgcattttc gagaagaccc aaatcatttg ctgccattcc
     3601 tagatatggg atgcttggct caactcaccg caccaagtta tgtcggtgta tttggaaaag
     3661 acatccagaa aacagccaaa atgatggtca aacataacct cgtgcaaatg gctgcttccg
     3721 atgcccatgg ggtctctaaa cgtacctttt acttaaaaga atgttatgag caaatcgcca
     3781 aggactttgg taaggaaaaa gtagcacaga tgaaacaagt cgcaaaggac ctgatcaacg
     3841 gggatgagat cagttatcca gtctatgaag aggtaaaaaa gaagaagttt ggtttatttt
     3901 agggggaatg tagggtgttt gtattaacaa gaagaagaaa aattgccaca ttggtatttg
     3961 tggacagctt gttgttgatt gctgcgaata ttgcagcagc taaattcatg aagccgtttg
     4021 tagctatacc aatggacctt attttaatct ctatcggttt gtcgattggt ttttatttat
     4081 tctatggtag tttattcaaa gtttttactc gaatcaatcg ttatacaaat ttaagagaga
     4141 tcattgcgat ttttggttcg ttatctgcaa gtgcggcctc aagtatcatc atattgcttt
     4201 tcataaataa acaatacagt ttacgtttag tgatctttgc atacctactt tcgctattac
     4261 tgatcattgg cagccgattg atctggcgta tctatatcga aacaaaaaat atgaggtatc
     4321 tatcaacaga ttccgctaaa aataccttga tcgttggggc cggcgaaggt ggacgtattt
     4381 tatacaacag ctttcttggt tctaaaacgg ctcaagatat ccatgtcatt ggattcgtgg
     4441 atgatgatcc gaataaaaga aatacctatc tttcaggaaa aaaagtactg gggtcattaa
     4501 aagatatacc ggaattgatc gaaaaatatg acatccaaat ggtaacgatc gctatccctt
     4561 ctttatcaag aaaaaaatta cgaagaatct tcgagttagt cgaatctgct catgtgaaag
     4621 tcaatacgat gccttctatg gaagaacttg cctcagggaa aatcagcgta tcaaaattaa
     4681 aaaccatcga cgtagtggaa ttactgggaa gagatgaggt agagttggat atcgaatcca
     4741 tcaaagacca gatcacaaac aaagtcattc ttgtcaccgg tgcaggagga tcaatcggtt
     4801 cagaaatctg ccggcaaatc atccaatttg accctgcaaa actattactt ttaggtcatg
     4861 gggagaactc gatttacttg attgaccgtg aattacgcac gcatcatcaa aagtgtccta
     4921 cagaaatcgt gccgatcatt gcggatatcc aagatcgcaa gaaaatcaat gagatcatgg
     4981 aacaatacca cccagatatc gtctaccatg cagccgctca taagcatgta ccattgatgg
     5041 aatataaccc aaaagaagca gtcaaaaaca atattttcgg tacaaaaaat gtagcagaag
     5101 cagccaaagc agcgaaagta aaaaacttcg tcatggtctc gacagataaa gccaacaatc
     5161 cgccaaacgt catgggttca acaaaacgaa tcgctgaaat gatcgtgact gggttgaatg
     5221 aagaaggatg tacgaaattc tcagctgtca gatttgggaa tgtccttggt tctcgcggta
     5281 gtgtgattcc agtattccgg gaacaaattg ctcaaggagg gccagtcact gtaacagact
     5341 ttcgtatgac ccgatatttc atgacgattc ctgaagcaag tcgtttagtt attcaatctg
     5401 gggcgttagc aaaaggcggc gaaatcttta ttttagatat gagtgaacca gtgaaaatcg
     5461 tagatctagc aaaaaacatg atccgattga gcggatatag cgaagacgag atcgaaatca
     5521 ttgaaacagg tatccgtcca ggggaaaaac tatatgaaga actattgtta gataaggaaa
     5581 gaaacgatga agcagtttac gagaagatct ttgtgggaaa catcaaaggt tattccatgc
     5641 aaacagtgat ggaatttgtc aagagcctgc cgcaagacga tgaacaattg gctaaagaca
     5701 ttgtcacttt tgcgaatgct tcgaataagt agggggagta aagtgaaagc aattattgta
     5761 ggagctggtg gatttggcaa agagattgct tttctactag aaagtttctc tagatatgaa
     5821 ttgattggtt ttgtcgatga ttcgttaaaa atgcaaaatc aagaattagt gggaaaaact
     5881 gtattgggaa caatagactc gttattagaa ttagaagaag aaactgctat ttttctggga
     5941 atcgcttcgc cagatatcaa agagaaaata tatcaaaaga taaataaaaa taataaattg
     6001 attttcccta atttagttgc atcaagtgca ctagtgggca taaatgttca gttaggaatt
     6061 g
//
LOCUS       DAXWLH010000143         6060 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXWLH010000143 DAXWLH010000000
VERSION     DAXWLH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.146"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            35..2632
                     /gene="adhE"
                     /locus_tag="ACGE36_002248"
                     /gene_synonym="adhC"
     CDS             35..2632
                     /gene="adhE"
                     /locus_tag="ACGE36_002248"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFF8384612.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2861..3967
                     /locus_tag="ACGE36_002249"
     CDS             2861..3967
                     /locus_tag="ACGE36_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8384613.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3984..5008
                     /locus_tag="ACGE36_002250"
                     /pseudo
     CDS             3984..5008
                     /locus_tag="ACGE36_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5014..5493
                     /locus_tag="ACGE36_002251"
     CDS             5014..5493
                     /locus_tag="ACGE36_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFF8384614.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5509..>6060
                     /locus_tag="ACGE36_002252"
     CDS             5509..>6060
                     /locus_tag="ACGE36_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFF8384615.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT         1949 a         1074 c         1367 g         1670 t
ORIGIN      
        1 taacaaataa ccatgaccca ttaggaggat aatgatggct aaagtaatag aaaaagaaaa
       61 aacgaagaca attgacgtac aagcaatgat tgatgatttg gcagaaaaag caaatgcagc
      121 attgaaagaa atggaaagct ttgatcaaga aaaagtagac catatcgtgc atgaaatggc
      181 aatggcagct ttggatcaac atatgccatt agcgaagatg gctgtagagg aaacaggccg
      241 cggtgtttac gaagataaag caatcaaaaa tatgtatgcg tccgaatata tttggcataa
      301 tatcaaacat gataaaacag ttggtgtgat caatgaagat gtccaaaaag ggttgattga
      361 aattgctgaa ccagtcggtg tggtttgcgg agtaactcca acaacgaatc cgacttccac
      421 aacgattttc aaatcaatga tcgcgttgaa aacaagaaat ccaatcgttt ttgctttcca
      481 tccaagtgcc caaaaatctt ctgcagaagc agcacgagtg gtacgtgatg ctgctatcgc
      541 tgccggagct cctgaaaatt gtatccaatg gatcgaacat ccttcgatcg aagcgacttc
      601 tatgctaatg aaccatccag gtatcgcgat cgtcttagcg acaggtggag ctggaatggt
      661 caagtctgct tactctacag gaaaaccagc tttaggtgta ggtccaggta acgtaccagc
      721 ttatattgaa aaaacagcaa aaattaaacg tgcagtgaac gatttgatcg tttcgaaaac
      781 atttgataat ggaatgattt gtgcttctga acaagctgtg atcgtggata aagaaatcta
      841 tgcagcagta aaagcagaat tccaagcaca ccaagtttat atcgtaaaac cagatgaatt
      901 gcaaaaatta gaagatgcag tgatgaacga aggaaaatat gcggtcaatc catcaatcgt
      961 tggacattct gcgatggaaa tcgcaaaatt ggcaggaatc agtgtaccaa aaggaacgaa
     1021 aatgctgatt gctgaattgg aaggcgtagg cccagattat ccgctatcaa gagaaaaact
     1081 ttctccagtt cttgccatga tcaaagctaa caacacggac catgcgtttg atttgtgtga
     1141 aggtatgttg aatcttggag gattaggaca tacagcagtt atccattcag aaaacgaaga
     1201 gttgcatgtc aaattcggtc ttcgaatgaa agcttgtcgt attttagtga atacaccatc
     1261 tgcagaaggc ggcatcggtg atatctataa cgaaatgatt ccgtcattga ctttgggatg
     1321 cggttcttat ggtaagaact ctgtttctcg taacgtctct gctgtaaact taatcaatgt
     1381 caaaactgta gcgaaacgga gaaataacat gcaatggttc aaattaccgc caaaaatctt
     1441 ctttgaaaag aactctctat tgtacttaga gaaaatggaa aacgttgaac gtgtcatgat
     1501 cgtttgtgac cctggtatgg tccaattcgg ttactgcgat acagttcgtg aagtattggc
     1561 acgacgtaaa aatgatgtga agatcgaagt cttctcagat gttgaaccga acccatcgac
     1621 aaataccgta tacgcaggaa cgaaactgat ggcagacttc aagccagata cagtaatcgc
     1681 tcttggaggc ggttctgcaa tggacgcagc taagggaatg tggatgttct atgaacaccc
     1741 agacacatct ttctttggtg caaaacaaaa attcttggat atccgtaaac ggacatataa
     1801 gatcgacaaa ccagaaaaaa cacaattcgt atgtatcccg acaacatcag ggactggttc
     1861 tgaagtaacg ccatttgcgg tcatcactga cagtgaaaca catgtgaaat atccgttagc
     1921 agattatgca ttgacgccag atgtcgcaat cgtcgatcct caattcgtca tgtcagttcc
     1981 agcatctgtc acagcagata ccgggatgga tgtgttaaca catgcaatcg aatcctatgt
     2041 ttcagtcatg gcttcagatt atacacgtgg attgagccta caagcaatca agttagtctt
     2101 tgactattta gaaaaatcag taaaaacacc tgatatggaa tcacgtgaaa aaatgcacaa
     2161 cgcttctaca atggctggta tggcgtttgc taacgcattc ctaggtattt gtcactcagt
     2221 cgcacataaa atcggcgggg aatacgggat ccctcatgga cggacaaatg cgattctttt
     2281 accgcatatt atccgctata atgcaaaaga tccacaaaaa catgcgatgt tccctaaata
     2341 tgactatttc cgtgcagata cagattacgc agatatcgca aaattcttag gtctgaaagg
     2401 cgaaacaaca gaggaattag tcgaagcttt agcaacggct gtttatgaat taggaaaatc
     2461 tgtcggcatc aacatgagtc taaaagctca aggagtcaca caagaaacac tagatacaac
     2521 cgtagaccgt atggctgaat tagcttacga agaccaatgt acgacagcta atccgaaaga
     2581 accattgatc tctgaattga aacagatcat cattgatgct tacaacggat aagcaaaaga
     2641 aagcgtagaa aaaagataag aaaatagatg aaaaagaact tggaaaacgt tgtttctcca
     2701 agttcttttt ttaatttcca agtaactgaa agttactttt gttatttaac atttaggtca
     2761 attttagaca gtttttgttc aaaaagcgat atttatagtg gacggtatta ctgctatatt
     2821 aaggatataa aatgaaagcg tttaaaaagg tggcgtaaag atgattatta aagaagagat
     2881 ttttgtacct aaagaattag tgaaagaaaa aattgacctg ctcgttgaaa acctgacaac
     2941 gatcaaagac gataatggtg aatttttgtt ggactttgat ggactgaaag tagatgacaa
     3001 aagctggaca gtgtggaatt ggccacaagg tgtcggtcta tatggtattt ataaaaatta
     3061 ccagatgaca aaaaatcctc gcgcttatca agtggtcaat gaatggtttg aggatcgtat
     3121 ggaagaaggc gctccgccca aaaacgtcaa tacaatggca ccgctattga ctatggctta
     3181 cttgtatgaa gacacgaaag attcacgcta tttgccttat ttagaacaat gggcagaatg
     3241 ggtcatgaat gacatgcctc ggacaaatga agacgggtta cagcatgcca catatggacc
     3301 ggaaaataag aatcagttgt gggacgatac attgatgatg acagtgcttc ctttggcaaa
     3361 aatcgggaaa ctgttgaatc gcccagaata tttagaagaa gctcgttacc agtttatgat
     3421 ccatatcaaa tacttgatgg ataaacgaac aggattgtgg tatcacggct ggacgtttga
     3481 aggaaatcat aactatgccg aagcattttg ggctcgtgga aactgctggt tgacgattgc
     3541 tattccagag atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt
     3601 gatcgaaaca cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt
     3661 atggcatacg ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt
     3721 tgcttacggt attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt
     3781 cgcttatcgt gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa
     3841 cgtttcaatc gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac
     3901 agcaatgcct tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta
     3961 ttgttaaaag aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc
     4021 ttagcctagg tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg
     4081 cagatgcaga cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt
     4141 aattgatgca atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca
     4201 gatccagtat ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca
     4261 gtcaggagca gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga
     4321 ttattcgatt ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat
     4381 ggataatgaa gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg
     4441 attgacctat tatgattctg gtcagcgcag cttttatatg gtagacggtc cgatccatac
     4501 gccggaagat ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat
     4561 ggtagagctt ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct
     4621 tcaatccaat ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca
     4681 tggcggggta gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat
     4741 catgaatgat gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc
     4801 agcgaaagaa tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa
     4861 agctttagca caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg
     4921 agaggcagtt gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta
     4981 tcaaaagatc caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta
     5041 gagatcctgg gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt
     5101 tccagaacgg ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt
     5161 gtctggtttg ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta
     5221 acggttttaa gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa
     5281 agtttatttc tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca
     5341 ttgacaatgg ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca
     5401 gtacctgtat cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta
     5461 agcaacaagc cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa
     5521 gccggattag tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct
     5581 ataagtattg cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct
     5641 gcttttagtt cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt
     5701 ccgtttttcg ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg
     5761 gattttgcga tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta
     5821 gggaatgctt tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt
     5881 ggagtgttga tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc
     5941 aatatcgcgt cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat
     6001 tcacttgttg ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc
//
LOCUS       DAXWLH010000144         6032 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXWLH010000144 DAXWLH010000000
VERSION     DAXWLH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.147"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..2259
                     /locus_tag="ACGE36_002253"
     CDS             <1..2259
                     /locus_tag="ACGE36_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFF8384616.1"
                     /translation="VVLGASRSGKGETFVVPLLDNLSRAKIQSNMVVNDPKGELFSAS
                     KETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWANKDYHEASKRANTLTSMLFASG
                     MGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNELGSLFYTDPKT
                     GKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLT
                     SKMSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCV
                     LNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEI
                     STLRLKYTDQPTAVFMVIPDYDPSNHVLASIFISQLYTELASNCKQTPDKKCFRRVHF
                     LLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQLETRYDKAFKTIKENCQNHIL
                     IMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVERASQLIEG
                     EQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHI
                     NLSLQDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYRNLSGSQQTINEEELLKL
                     VNTEVKSQDMEGQVLSEQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTIN
                     SQVLDTPFIQSLINDSIAIHNVQESKDQIMILEQQLANAYELAVLKTKNSILKNKIIK
                     LKNAMTKLAKAI"
     gene            2277..5051
                     /locus_tag="ACGE36_002254"
     CDS             2277..5051
                     /locus_tag="ACGE36_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8384617.1"
                     /translation="MTESINWRKLNVQWLKYKWPIIVSLIIILFMAYPQRVFALPWIT
                     DNKEVLKFLTKYQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFS
                     FVESSSLGAVYQSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALIL
                     LMPTMISSGIQFAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDG
                     VQKNALKEKNFFSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDG
                     WMSMFSDSFKGGYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVA
                     YVCAVFVLIKGSGTVMRYFGVDIGLKEGYGQLVSAFGMGAMLFRKGSNGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQN
                     STSSQGFKSVDVRENVQGSSASQAKTVDVRENVQSSTSSQGFKTVDVRENVQKDSGEM
                     KEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLN
                     DPNPLFDNLN"
     gene            5075..5410
                     /locus_tag="ACGE36_002255"
     CDS             5075..5410
                     /locus_tag="ACGE36_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5592 family protein"
                     /protein_id="HFF8384618.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            5407..6024
                     /locus_tag="ACGE36_002256"
     CDS             5407..6024
                     /locus_tag="ACGE36_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384619.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
BASE COUNT         2235 a          800 c         1228 g         1769 t
ORIGIN      
        1 gttgttttgg gagcttcacg atccggtaaa ggggaaacat ttgttgttcc tttattagat
       61 aatcttagcc gagcaaaaat acaaagtaat atggtcgtga atgatccgaa aggagaattg
      121 tttagcgctt caaaggaaac acttgaaaaa cgaggttatc gtgtattagt gctaaatatt
      181 gatgatccac ttgaatcaat gagttttaat cctttacaac tagtaattga tagttgggct
      241 aacaaagact atcacgaagc gagtaagcga gcgaatactt taacttcaat gctctttgct
      301 tcaggaatgg gtacagataa tgagtttttc tataaatcag ctaaatcagc ggtcaatgcg
      361 attattttaa cgattgtaga acactgtttt aacaatgatt gcattgaaaa aattacaatg
      421 tacaacgtgg ctcaaatgct gaacgaatta ggaagcttat tttacactga tccaaagact
      481 ggaaaagaaa aaaatgcttt agatgaatat ttcaatactt taccacaagg gaatcaggcc
      541 aagattcagt atggttctac ttcatttgcc ggtgataaag ccaaaggttc gattttatct
      601 actgcttcac aaggtattga aatgtttaca agtgatttgt tcgggaagtt aacaagtaaa
      661 atgtctattg atttgaaaga gataggcttt cctaagtcca tacaatttaa agtcaatacg
      721 aatttagttg gaaagaggat tagtatttca tttttgagaa aagaaaatga tgtaattaga
      781 ttaattaaga agtatcgagt gaaagtaaaa gctttagggc tatgtgtttt aaattttaat
      841 gaatatcttc aggatggaga tatgttggat atacgatatg aagaagagaa atcgagagca
      901 tggtatcgta ttgaatttcc caaaaaacaa gcaaatagtc atgatacttc tttagtcact
      961 aaaaaattca agtcaggtaa aaattattct gacttagaaa tcagcacctt acgattaaaa
     1021 tacacggatc agccaactgc tgtgttcatg gttattccag attatgatcc gtcaaatcac
     1081 gtacttgcaa gtatttttat cagtcaactt tatacagaat tagcaagtaa ttgtaagcaa
     1141 acaccggata aaaaatgttt taggcgagtt catttcttgt tagatgaatt tgggaatatg
     1201 ccggctatag acaatatgga tgggattatg acggtatgtc taggaagaaa tatgcttttt
     1261 gacttagtga ttcaatccta tagtcagcta gaaacaagat atgataaagc ttttaaaaca
     1321 attaaagaaa attgtcagaa tcatatttta atcatgtcga acgatgagga aacgatagaa
     1381 gagatttcaa aaaaatgtgg tcataagaca acaataaata gaagtgctag ttctaaacat
     1441 ttagatactg atagctcggt cactagttcg gcagagcaag agcgagtaat tacagttgag
     1501 cgtgctagtc aattgattga aggggaacaa attattttaa gaaaccttca tagacaagat
     1561 acaaaaagaa ataagattag accatttcct atttttaata ctaaagaaac aaatatgccg
     1621 tatagatacc aatttttagc agaagacttt gatactcaaa gagatattaa tgagattgat
     1681 attgcatgtg aacatatcaa tctatcttta caagataacc aaattccata tgcaaggttt
     1741 atcaaagatt taaaaacacg tttggagtat tcaattatta ataatattcc tattagtgaa
     1801 gaagactatc aggattatcg aaatttatca ggatcacaac aaacaattaa tgaagaagag
     1861 ctgttaaaac tggtaaatac ggaagtaaaa agtcaagata tggaaggtca agttttatca
     1921 gaacaagaaa tgaaaattaa tatttatcta aaggaaagtc aaaaggaagc aatagaatta
     1981 gtaaaagtaa ttaatggaag gttagctgat gaaaattcag cagatatggt tataaaaacc
     2041 attaattcac aagtccttga tacgcctttc attcaatctt taatcaatga ttctatagct
     2101 attcataatg ttcaagagag taaggatcaa attatgatcc tagaacaaca attagctaat
     2161 gcttatgagt tagcagtgtt aaaaacgaaa aattcaatac tgaaaaataa aattatcaag
     2221 ttgaaaaatg ctatgactaa gctagcaaaa gcaatttaga aagaagggaa gaaatattga
     2281 ctgaatctat taattggaga aaactaaatg tgcaatggtt aaagtataaa tggccaatca
     2341 tagtttccct aataatcatt ctatttatgg cctatccgca acgagttttt gcgttgccgt
     2401 ggattacaga caataaagag gtattaaaat ttttaacaaa atatcaagat tatcttcagt
     2461 ttactaaagg tggttttgga agcattgtcg ttcaaagttt atcgtggggg ctgattagag
     2521 gtgcatattt tgtttgtaaa tcagcagaaa accttgttcc acaatcgcta agtttgttta
     2581 gctttgttga aagcagtagt ttaggtgctg tttatcaatc tgtattaaac acggtggttg
     2641 taggattaat gatcctttca ttgatgtttg ttggttacaa aatgattact ggaaaaggaa
     2701 cagtggattt aaaatcggtt ggaatgaacg tagtgatgtc tatagcatta atacttttaa
     2761 tgccaaccat gatttctagt ggtattcaat ttgctaaaac attttataag gactctacta
     2821 cgattacaaa agctgatggg ggcgttgctt ggtcattgat tgaggacggt gtaactgatt
     2881 tagtatatat caataaaaac gatgcttatg atcggttggg taaagatggc gtacaaaaaa
     2941 atgcgttaaa agaaaagaat ttcttttcct acgatttcag tgaagtaatc acagttgata
     3001 cagctaaaga tttagaagat gataataaaa aagctgaata tcttcaatct aggttgacac
     3061 cagatggcga aggaaatatg caagccgtta agattgatga tggttggatg tcgatgttct
     3121 ctgatagttt caaaggaggg tactacaggt ttactaaaaa tacttttgca ttgactttag
     3181 gattactttc gttagctgta gcctatgttt ttagtatttt tatcatagtc accgctattt
     3241 tagaactggc atttaagcgt gtgttagggg ttcttgtttt tgcaacagat attgagacag
     3301 gacagcgttc aaaagttgtt ctatcagata ttctacagtg ctatttgact gtaggctttc
     3361 aaggatttgg attatccatg tttgcaatgt ttgtttccta tttaggaaca ggcggagggt
     3421 cttccgttaa tccgtttatt aaggctgtag cttatgtatg tgccgttttt gttttaatca
     3481 aaggcagtgg aacagtcatg agatactttg gtgttgatat tggactcaaa gaaggctatg
     3541 ggcaacttgt ttctgctttt ggaatgggtg caatgctgtt tagaaaagga tcaaatggat
     3601 ttaatcgtgc caaaggaagc ggaaacggaa atggatcaga cagcggagaa ggagaagatc
     3661 gaaaaccaga aaagaatttc ggtgaaactc tatcaaaaaa agctggcaaa actggaagag
     3721 ctttgggata tgcccatgaa cgtggattat caggtttagc ttcagatgga gctacaatgg
     3781 ctagtgaaag agcaacaaaa ccatttaaga gtatgcgtga tatggctaat gatactaaga
     3841 ataagttcaa agaaggttta gatgatggta cagtgtccgc tattaataaa aatagcaaac
     3901 ctatgttagc taaaaataaa gaagatgaaa ctggaaaata tgctgattcc atgccaacac
     3961 gtctttcgga tagaaaagat ggtgctaagg ttgaaaatgc cgatagaatt atgtctagtt
     4021 ctgaacgtat gcgtgaagca atgaaaaata atgctgaatc gaataataat cctgtaagtg
     4081 caattcagca aaaagttcaa caagatattg aagaacgtaa aaatgctatg cacggtcaag
     4141 ctaagtctgc tgaagaatta attaatcaga agcgacaaga agctaaatac acgcctgaag
     4201 caatgaatcg tgaggaaatg ttaaggaaac gtgtagaagg gcgtacagga actaatacgg
     4261 acgaaaaaga agcgttgacc aaagaaagaa tccaagaagc taaaaactca aataccggat
     4321 tagaaaaatt ggtcaaagaa aatctacaaa attctacaag tagtcaaggt ggtcgatcag
     4381 tagatgtccg agaaaatatt caaggttcat tgaatggtca tgtcggacgg aatgttgatg
     4441 taagagagaa tttacaaagt tcttcaaatg gtcaaggtac taagacaatt gatgttcgag
     4501 aaaatctgca aagtagcagt acaagtcaac caaaaacagt agatgttcga gaaaatgtac
     4561 aaaattctac aagtagtcaa gggtttaaat cagtggatgt tcgagagaat gtgcaaggta
     4621 gcagtgcaag tcaaccaaaa acagtagatg tccgagaaaa tgtacaaaat tctacaagta
     4681 gtcaagggtt taaatcagtg gatgttcgag agaatgtgca aggtagcagt gcaagtcaag
     4741 caaaaacagt agatgtccga gaaaatgtac aaagttctac aagtagtcaa ggattcaaaa
     4801 cagtagatgt tcgggagaac gttcaaaaag atagtggaga aatgaaagaa aaaacacaaa
     4861 aaatcaatat cgttgaagac agaaagtcag cgaaaaaatt tgattcaaat catgaaacag
     4921 tgattattga tagtgaaatc cgtgaaaatg ataagggatc aaaaccaaga cgaagattca
     4981 cttatgaaga caacgaatta ttcagagata cacttaatga tcctaatcca cttttcgata
     5041 atttaaatta aattaaaagc cggcatgagt gattatgccg gctttttatg aggtgaaaat
     5101 gatgtataga aatcccaaaa atataaaaca agagataaag ctatttttct tctatttagt
     5161 cgatttagga atagtagccg gaagcgtgat ggcaagtgtt tactttgcaa aattaatgcc
     5221 aatttcacca atgatgaaat ttgcactgta cttattagca tttgtagtgg gtgtgtggat
     5281 gtgcgttaga acgcctaatc accctaaaga gcgaaatatt ttaatgctgt attatctctt
     5341 taaaatggat agaaacagat accacgaagt agagtttaaa agaaatagta gggggaaaat
     5401 tgaattatga ttgttgctga aagtaatgaa aataaattaa gtggtgtttt tgacaaattc
     5461 ttagaactag ttattccaaa agataaagat atgtgtgaac ttttaccata taaaaaaata
     5521 ttgaaagacg gaacgttagt gactaatgac aataagtatc aagccttttt taaagttaaa
     5581 acaactgact taacttctat ttcagaagat ggatttatgc gtatggtaaa caaactcact
     5641 tatttaaatc gagtatattt agaaccatat aaaatttatt cattaactta tcctactgaa
     5701 acagtaaatc aagcgattta ttggaagcat agaatacagg aggcaaagag aagtttattg
     5761 agtaataatg atcccaattt agaaacgtat ctgaagtgga atctaaagct agcttacgat
     5821 aattatgaaa gagttgatct agtatcgact ttaccagagt tgatgttcgt tatttgtgtt
     5881 tatggaaaaa cacaaaaaga agtgaaaaat catgccgatg taatgaaaca ggccggtggt
     5941 agagaattag atttacaaag aattactgaa ggggatttaa tcaatgtgat aactaaattg
     6001 atgaatccca atacttcttt ataaggcggt gc
//
LOCUS       DAXWLH010000145         6012 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXWLH010000145 DAXWLH010000000
VERSION     DAXWLH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.148"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(78..584)
                     /locus_tag="ACGE36_002257"
     CDS             complement(78..584)
                     /locus_tag="ACGE36_002257"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8384620.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            744..1184
                     /locus_tag="ACGE36_002258"
     CDS             744..1184
                     /locus_tag="ACGE36_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8384621.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(1210..2367)
                     /locus_tag="ACGE36_002259"
     CDS             complement(1210..2367)
                     /locus_tag="ACGE36_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384622.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2370..2726)
                     /locus_tag="ACGE36_002260"
     CDS             complement(2370..2726)
                     /locus_tag="ACGE36_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384623.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(3024..3998)
                     /gene="sgrA"
                     /locus_tag="ACGE36_002261"
     CDS             complement(3024..3998)
                     /gene="sgrA"
                     /locus_tag="ACGE36_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFF8384624.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(4194..5033)
                     /locus_tag="ACGE36_002262"
     CDS             complement(4194..5033)
                     /locus_tag="ACGE36_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8384625.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            5218..>6012
                     /locus_tag="ACGE36_002263"
     CDS             5218..>6012
                     /locus_tag="ACGE36_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFF8384626.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTL"
BASE COUNT         1923 a         1195 c          884 g         2010 t
ORIGIN      
        1 ttattttctc attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt
       61 ttaaacgaag aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc
      121 tcgtaaaaag cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct
      181 cctctacaca tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata
      241 caatactctg cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa
      301 acactcaaat tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc
      361 tcaggaatag ataattccga caatagctcc catggttttg tttgtggcac taattgagca
      421 tcttcgtgtt gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta
      481 ttcactaatg ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct
      541 ttttgtaaat cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc
      601 ttttttcttg atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt
      661 tcttatttta cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa
      721 atacttagct attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt
      781 cttcaagacg cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact
      841 taattcgtca accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg
      901 gcgtgaccga aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag
      961 atgaactcag tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa
     1021 aaccgattat ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca
     1081 ggatgttcct ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc
     1141 atcttgtcga ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat
     1201 ctgcttttct taattaacga aatccaattt ttccaatatg gatctatttt tctcattcct
     1261 gatttttatg atttcagctt tcagacgcgc ccaatcattt tttgtaggtt gtccattcca
     1321 gtaaaatatc tgtgtattcg tttgatcata tgcctttttg tcgataggat aatcgctaat
     1381 gacaatatca ggtaactgat cagcggatgc actatatttt attgggcgtt cagtcataga
     1441 taatatcctt ttctttgccc ataattcctg tgttttacca tactttgatt gaatatagaa
     1501 tatgacaggt tgttcgtgtt ttataaaaat atccctgact aacatacagt attgaaaaat
     1561 caaccgtctg ttcttttcat aataagtatt aagctctgga attctgcgag ctaccccact
     1621 aaaaaactgt tgcatgattg gatacatcac tggaaatata tgctgaaaat ctctgtcttc
     1681 tgctgatttt ccaaaagcat ccaccttctt ttttgtccca aaaacccttg aatacaaatg
     1741 gatcattgcc aaattgacat atagataatt tttttctgaa gttgtaaaac aaatcttgaa
     1801 gtttttttca aaattttcta caatagacga ttcaatccag ccccaacttt cttgtacttt
     1861 atctggagta tccaacaaat cttcttccaa ataggtgttc attacaccaa acataaaata
     1921 aaggtaagct tgctctgttg gagtgatgtg attcatcgac cagaagtttg cttcaaaaaa
     1981 aggctctagt atgtaatgcg taaactcttc ttttgttatc aatggatgca caaatttgtt
     2041 catctcttct gggacatacg taatcgagtg gccttgtttc attcgttgca acgaaattct
     2101 tagtaggctg atccatttct ttctatctat actacgcgta tatggcgcat gtttcgcaaa
     2161 tttttctaat agttgacgtt tattttttat acttgataat atatcagaat gattttcgtc
     2221 gaatgactgc caatacatca agtgaaaaaa gtatcggata cggtattctt ctccttctat
     2281 cggattacct tttttcaagc tgcaagataa atgatagttt ttcaaaatag tattaagttc
     2341 tcttaatttt ctttgaatcg tacgcaaact tatataattt tttgactaag attttctaag
     2401 gattgataag tacctttgta aagtgaatcc agtaaaatat aaccaatcgt tccctctaaa
     2461 agtatagaat aaactttact cagtaaaaac tgagaagaaa actcgacttt tatcatcttg
     2521 ttttctgaaa gaaaagataa tttatattct ggataccttt cgcaaaacga ttctgcttgt
     2581 tgaatcaccg aattcatctt agccagggtg tagtttagat ctaaagcgat atccttcacc
     2641 acatataatt tctcgtctct ttcataacaa tagttcaaaa attgatatac ttgcctttca
     2701 ttgatatcaa taaattcttc aaacaaagta catactcctt tttatctatt atttagactt
     2761 attccgacag ctacatgaat aacgtacaaa tagattcgtt actgattttt cagagatact
     2821 accatgcgca gtgtgcattg tcaattattc ttgctcttat tttccatttg tgttcctgtt
     2881 atttcctgtt tctcaaatat gttagtaaac aaaaatagat ctggcaaagc tacaacttcg
     2941 ccaggcaata cagcactctc gaatgaaata ttcactcaaa aatctatcgt tttattcaat
     3001 tttagatctg ttgcttttat tctttatttc actcgatgga agagagaacg aaaatagctt
     3061 gctcctacaa tcaaaatgag taacacaccc atccatgtaa ttagcttgtt caaggttcta
     3121 ctaccagttt ctggtaaatc gctttttcta ttatctgttt ctttcgcttt ttcttgatga
     3181 tcaattccac gagaaacaaa atcaggctgt tttagcacac tttttaggtt gctccctgca
     3241 tttctctttt gttccttagt tggtatgata ggtaccaaat caagttcatt atttactttt
     3301 cctgtgctgc tttcgctgct cgtgcttcct gtgctactct cactactcat gcttcctgtg
     3361 ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgcttcc tgtgctactt
     3421 tcactactcg tactctccgt actgctttcg ctgctcgtgt tttctgtgct aatttcactg
     3481 ctcgtactct ctgtactgct ttcaccgctc gtgttttctg tactgctttc actacttgtg
     3541 ctatccgtac tgcttggcgg tccaacaact gtccaactgc ttggtttcaa attctccgtg
     3601 atcgtcatct gtccatcata aaaaacaatc aaatcaactt tatctttact cttgatctca
     3661 tcaatcgata gttctgcaga aatcattatt gtatactcaa taccagcggt ccattgtacg
     3721 tcttttaaat aaacttcccc aataactttt tctgaacttc cattaccatc ccaaccatat
     3781 gttagaacag catgtacatc actagcattc acgcttttgt ttggtttgac tggaatagta
     3841 atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt tcacagtaaa tgaaagattc
     3901 tcatttgccc gttcatttgc agaagtactg attccaatca ttgctgcaat tatactcagc
     3961 ccaagaataa caaataatac ggtcgctgtt ttcttcattt ctatcactct ccatataatt
     4021 accttctatt tgataaagga attatataaa atgaaaatga tgagaacacg acagtcagtt
     4081 gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat
     4141 caatgacaga cttaaaaaag caagaccgat atcagccttg ctttcttcat cttttattga
     4201 taaacagcag tgtatttctt catccgcttt gttttatatg catagagtaa caaaccaaca
     4261 atcaagacta acaagaataa tgtagttgat atagatgctt cagccccaaa gtttccccca
     4321 gtaattattg tattttgcga ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg
     4381 ctgactggta tacccaaaat agctccttgt gtccaattcc aagtaatatg agcaccaata
     4441 gccgtaaata aacttcctgt actgtagata agtgcgctga aaaagcaacc tgctaaaaag
     4501 atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat
     4561 gcagcagatc caatcattgc tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag
     4621 ccacggaata aaagctcttc tgatgccccc actgcaataa ataaaataaa actcgttatc
     4681 agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg cccccgtcaa atataatgga
     4741 ctagtaataa gcagaaacac tccaattcca acaacgagac ctcccgctaa atacccccag
     4801 tcttttcctc gaacagaaac accataatct tctattcttt ttccttcgac cttactagca
     4861 agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg taccggcaat tgctccaatc
     4921 cattcactat ttccaataaa catatccgca agtgcattat tgacaagaat tcctaaaaca
     4981 aaaccaacaa agactattaa aacagatcca atcaaccgaa taatcctgat catctttcat
     5041 ccctccttta tattaagttt aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa
     5101 tgttctccat catagagaga atagattttt acgaactaag aatatctaca gcaaccttac
     5161 gaaaatgaaa gactgtctcc ctaattcttc tgttagactg tttttaataa ggaaaggatg
     5221 ggaaattttt tgaaaaaaca actactaatt atgtgccttt cagcatgtgc aattttacta
     5281 ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt ttaaaggtgg aacacttaca
     5341 gttcaggatt tttatgataa cccaaaagtt aaatcaaata atcaaacact attaaaaaaa
     5401 atgattgtct tcaaagcatt tgatgatgta tacggaaaag agatttcttc aaaggaagta
     5461 acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa actacaagga acaattaaaa
     5521 gcagttggcc aaacagaaga gacatataag aaactcttta aacagatgct ggctttccaa
     5581 tacggtttga aagccaatgt aaaattaaca gataaagatc tagacactgc ttggaaagaa
     5641 ttctatccag aagttagtac acagatcatt ctattttcga ctaaagaaga agcagataaa
     5701 gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc ttgttcaagc ttatggaaaa
     5761 aataaaacat taaaggaaac tgatggaaaa atgaattttg attcaaccaa tccagagatt
     5821 cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt
     5881 cctgtaacag atccaacgac ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa
     5941 caggataaag gttctaataa agacaaatac aaatctgagc tagaaaaaat tgctaccgaa
     6001 gcaaaaactc tt
//
LOCUS       DAXWLH010000146         5939 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXWLH010000146 DAXWLH010000000
VERSION     DAXWLH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.149"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            68..676
                     /locus_tag="ACGE36_002264"
     CDS             68..676
                     /locus_tag="ACGE36_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8384627.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            844..2481
                     /locus_tag="ACGE36_002265"
     CDS             844..2481
                     /locus_tag="ACGE36_002265"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFF8384628.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2553..3665)
                     /locus_tag="ACGE36_002266"
     CDS             complement(2553..3665)
                     /locus_tag="ACGE36_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8384629.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3740..5113)
                     /locus_tag="ACGE36_002267"
     CDS             complement(3740..5113)
                     /locus_tag="ACGE36_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8384630.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5334..>5939
                     /locus_tag="ACGE36_002268"
     CDS             5334..>5939
                     /locus_tag="ACGE36_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8384631.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKI"
BASE COUNT         1996 a         1180 c         1195 g         1568 t
ORIGIN      
        1 aatatttcta aatgataaag ttaatagcgt cgaaagaata aaaaacggag aaaaaaagga
       61 gtttaatatg aattcattta agaaaattgt tttaggtaca acttttgctg ctggtgctac
      121 tgcaatgttt gtaggaacta caaatgctca cgctgatgaa gtttatacag taaaatcagg
      181 tgattcatta tctaagatct cacaaaaatt tgcaggagac aactcaatga tcgatgcaat
      241 tgctgaaaaa aattcgatcg caaacatcaa ccgcatttat gttggtgaac aattaacaat
      301 cccaacttca aatgattctt cagcaacaac agaaaacaaa acaacagaaa atacagcatc
      361 tacaactgaa acagcaacgc aagaacatac atacgttgca cctgttgaaa cagttgaagt
      421 agctcctgct gccccagctg ctgcaacagc gccaacttca agctctgcaa aagaatggat
      481 cgcacaaaaa gaatcaggcg gttcatacac agctactaac ggacgttaca tcggccgtta
      541 tcaattagat gcttcatact taaacggcga ctattcagct gccaatcaag aacgtgtagc
      601 agaacaatat gtaacatctc gttatggttc atgggatgct gctaaaacat tctggttagc
      661 aaacggctgg tactaagccg ataaaaggaa aatgatgaaa ccgagaaact tgtttattca
      721 agttctcggt ttttttgtgt gaaaaataac tcgtttgctt cccacagcgc agcagtacaa
      781 ataaaaactc gaataatgta taataaagaa aacgtttttt tgtttaggga atgaggagga
      841 gccatgagta caaaagagag tctcgaatta aacgaaatca caagtggaaa tccactgtta
      901 tccagtattc gatcgattgt agaaacgaca ttttacggta ataatgttca tgaagttttt
      961 gataggaaga cagcttatca actagcaaaa gggagtccag gaacgatcat cacagattta
     1021 acaatctcac atgctgaaga actagattta ccagctgatg ttaggacact agtattcaat
     1081 gatggaagca ttgtgggaag aacagcaagt gctcggcgga tctttgagga cttagacaag
     1141 gaacaaagca aatacgagaa aattttaaga gaagctgtgt atcaaagtcg caagcgccag
     1201 ttttaccata caaaagtaat cgttggccta tctgaagaat tttctgtgca aagtcatttg
     1261 ctgattccag aaggatttga gaacaatttg tattcttatt tgctgaattt tcagccagcg
     1321 aattcgaaga tcttagagac ttatcaaaag tctgtttcct ttgatgaggg agatctttat
     1381 atcttttgtg atcccgaatg gacacatgaa acatttccaa atggactcat tttgtttgat
     1441 gctctccata atacagcggt tgtgctagga ttacgttact ttggagaatt aaaaaaagcg
     1501 actttgacgc ttgcttgggc attgggaaat cgcaatggct ttattgcctg ccacggtggg
     1561 atgaaacaat atgctagtaa tgaccagacc tatacgatgg ctgtatttgg gttgtccggt
     1621 tctggaaaat caacgattac cttagcaaaa catacggaag gtcactctgt ttcgatcttg
     1681 catgacgatg ctttcgttat ttctcgaaaa aatggttttg ctactgcctt ggaaccttct
     1741 tactttgata aaacacaaga ctatccgtct gatgatccgg ccatttcttc tttccttact
     1801 tgtcagaatg tgggcgtgac atgtacaagt aaaggagaaa aagtcctagt aacaggagat
     1861 atacgaaacg gaaatgggcg gacagtcaaa tcacgttatg cgacacccga ccgagtcgat
     1921 catcttagcg aaaagatcga tgctgttttt tggatcatga aagatgattc gcttccgcca
     1981 attatcaaga tcaatgaccc agttttagca gcgtcttttg gtgcaacatt agcaacaaaa
     2041 cgatcagacg cggaaaatat cactacaaaa agacagctag atgagctagt cattgaacca
     2101 tttgctaatc ctttccgtgt ctatcctctt agggaagact atcaagcttt tcgagagttg
     2161 tttttgaaac agcaaacagc ctgctatgtc ctgaatactg gatatttcga tgataaaaaa
     2221 atcaaacctg aaacgacatt gtcaatcgtt gaaagaatcg tgcaacaaac ggcagaattc
     2281 aagtcgtttg gtccatttga agacatccac tatttaccta taccagattt tcctgtttct
     2341 tttgaggata cgtcctataa aaatctggtg aaacaacgac ttgatattcg tttacgattc
     2401 attgaagctc agataggaaa agatctcttg ccagatgaag caaaagaaag tttggagaat
     2461 ttgattcaga aattgaacta aaataacagc tttttcaaga agtaatccat gcgcaatatc
     2521 gctcgggatc cttacttgaa aaagctgttt ttctatgatg gatttttcgt aatataggtg
     2581 gagatgtcct tttgataatc ttccgtaggc caatgaccag tatttaagaa gttggctaat
     2641 agatagccga tataaggacc tgtggtcaag ccagaagagc ctagaccgct agcagccagt
     2701 aagcttggat catctggcag tacgccgaaa aatggggaaa aatctgacgt ataagcacgg
     2761 gtacctacac ggaactcgta tggccagttt tttatctgat ctggatcagt caagaactgt
     2821 tctgtactct tagtcaatcc cttataagct tcgctagtag gagccaaatc ccagccttga
     2881 tcgttttcat gggtagcacc taaaaggatc tttccatcca tgaaaggaat gaaatcagct
     2941 tctccgtcaa gcatagcaac aggccagtct tgactcatct tataagatgt ctgaaatgct
     3001 aagagctgcc ctttttgcgg acgtatatct gcagcatagc ctaattcttg taataaaggc
     3061 ttaagtgccg ggccaggtgt caaaattaca cgatcagctg tgtattctcc aaacggtccc
     3121 ttgatgagcc aagaatcgct cttcttatgc aaagtgactt ttgcctggat ctgctccatt
     3181 tgtttttgtg ctaaacgctg accaagatgt tctaaatatg cttttccatc cagacgacca
     3241 ccaccactga tacgcagtga aactgctggt ttcaataacg gaagtgcttg agatgtctgt
     3301 tcaggtgaaa gccattcgat gtttccaatt tctggcgctt cttcttttcg tttctcggca
     3361 agaacagcca attcagctaa atctttttca ggtcgaagca aaagtgtccc tgattgtgcg
     3421 taaatcgttt gatcaagttg gaaatctttt ataagtttag ggaaaaaagc agcgccgtct
     3481 ttagccagac gataccattg tttgtttctg cgtttcgaca accaagggga gataatccca
     3541 gcgctagctt tagttgcctg tcctgtccct tcatcaaata aagcaacatt gaattttgat
     3601 gtatctaagt aactggctag ggtcatacca atgatcccgc caccgataat ggcaattttt
     3661 tccataaaaa agcctccttt tacaaacaac cattttcaca caaaaaaacg gcagatgcaa
     3721 cacctgccgt atatggaatc tacgcttttt tagcaaagaa cagataatag gcgaatcgag
     3781 caaagcttac gagaagcaac agcaacaaga cgcctaatgc tgcttttgat cctaatgtag
     3841 tagacacttc atagttatgg gaagtatgat gctggacgta attgataatg accgctacta
     3901 atgaagtagc cacagcgcca gaaaactgct gcaaggtatt gaacattgcg ttcccgtctc
     3961 cttgtagttc ttcattcagt gcattcattc cggtggtcat catattgctg taagataatc
     4021 cgataccgat catataaaac acatgacaag caatcaatac acccactgaa gcgtgtccta
     4081 tgagtaggaa taatgctagc caaccaatca aagaaagaat cagcccagca aaaattggct
     4141 tcttcggacc atattgatcc agcattttcc cggaaaaagg agaaagcaaa gcaccaaccg
     4201 ctgcgccggg aagcatcgct aatccagcga caaaaggact acttccttga acgatctgaa
     4261 ggaaattcgg caagacaaaa gatacaccaa gtaataaaaa ctgataaact aagaagccaa
     4321 tcaaaaacaa gcggaatgtt ttatttgaaa gaatagacag tcggatcagt ggatgctcca
     4381 cttttttact tcgatagtaa aaaaggaacc agccaatacc gccgatgatt aaagcaatcc
     4441 agctggaaac tgttcccaac gtactcaaaa atgttagact tccactaaac aaaagacaaa
     4501 ttcctaaaac gcttatccaa tccaaataac cacttttttg tactgccatc tcgggaatag
     4561 caaaccagcc gataatcaaa gaaagaatca aaacgggcac aagaaacagg aagatatgat
     4621 tccatgtaaa atgtgaagtc ataatgcctc cataagtggg gccaattgct ggagcaatgg
     4681 ccgttgtcaa agtaccgaca cccatcatcg cgcctcgtcg attgaggggg gcaaatgtca
     4741 agataatatg gaacatcaac ggaagagcga tacctgtcgc tgcaccttgg agcaatcgac
     4801 caaacaatag gaccatgaag gtaggagaga agaaatcgat cattatacct gccaagaaaa
     4861 gcagatttga agctaaaaag agttttttga gactaaaatt gcgattcaaa taagtagaca
     4921 gcggaacaat gatcgaaatc atcaaaagat aaatcgttgt tacccattgg acttgtgcag
     4981 tcgttaaatc aaactgtctg ataagagttg ggaaagttac attcatggcc gtctcgatca
     5041 atacgcccgc aaaagacatc atccctgttg caattactgc aggtactaaa agcagctgtt
     5101 tttgttttgt catttacatc acctctaaaa aatttcgtaa aaaaagagag tgatttctat
     5161 tataaaaata gaaaccgcct ctctctcgac acgccgaaac gtgttatctg tacgatttat
     5221 ttacttcact tattctagca caaactgaaa aatagtgtca acacgattag ccaagtcact
     5281 caaaaagagg tatgataata aataactaaa tggaatttcg cggaggaaga aaaatggaaa
     5341 aaattgaatg gcgtaaagac aagagctata gtatgaaaaa aagcccgcaa atactagagg
     5401 ttcctaaaaa atcattcttt agcatcagag ggatcggcga cccgaatcag gaagacttta
     5461 aacgaagagt agcctgtcta tatgcagtca gctatgcaat acgcatgtcg cctaaaaaaa
     5521 attggctgat tcctgattat cagatgtata ctgtctatcc actggaagga caatggggac
     5581 tgcaagaaaa gtatttgcat gaaccagtga tgaaaaaaga acatttttct tatcaattga
     5641 tgatccgaca gcctgacttt gttacagaag cgatagcaca agaagccatt cagcgttcta
     5701 gcaaacttcc ggaagacttg aaagaacaag tagtattcaa aaagatggaa gaaggtcttt
     5761 gtgcccaaat cttacacgtc gggccctatg ccgaagaacc agcaacgttt gagaaattag
     5821 aagcattcat cgcaaaagaa ggctatgaaa gagcttctaa agaacacaaa gagatttaca
     5881 tcagcgatcc aagaaaatca gcacctgaaa aaatgaagac gattttgcgt gttaaaata
//
LOCUS       DAXWLH010000147         5854 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXWLH010000147 DAXWLH010000000
VERSION     DAXWLH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.150"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(170..3319)
                     /locus_tag="ACGE36_002269"
     CDS             complement(170..3319)
                     /locus_tag="ACGE36_002269"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFF8384632.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="ACGE36_002270"
     CDS             complement(3333..4550)
                     /locus_tag="ACGE36_002270"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8384633.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5854)
                     /locus_tag="ACGE36_002271"
     CDS             complement(4540..>5854)
                     /locus_tag="ACGE36_002271"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8384634.1"
                     /translation="EYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDL
                     QSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEF
                     YTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELN
                     TTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADT
                     TFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQ
                     KLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKL
                     SEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVG
                     NDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1597 a         1246 c          978 g         2033 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttcgtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctct
//
LOCUS       DAXWLH010000148         5759 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXWLH010000148 DAXWLH010000000
VERSION     DAXWLH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.151"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACGE36_002272"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACGE36_002272"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFF8384635.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACGE36_002273"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACGE36_002273"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFF8384636.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACGE36_002274"
     CDS             2591..3376
                     /locus_tag="ACGE36_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8384637.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACGE36_002275"
     CDS             3389..4240
                     /locus_tag="ACGE36_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8384638.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACGE36_002276"
     CDS             complement(4364..4816)
                     /locus_tag="ACGE36_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384639.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACGE36_002277"
     CDS             complement(5281..5649)
                     /locus_tag="ACGE36_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFF8384640.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1942 a          932 c         1165 g         1720 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgat
//
LOCUS       DAXWLH010000149         5724 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXWLH010000149 DAXWLH010000000
VERSION     DAXWLH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.152"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            68..1012
                     /gene="mvk"
                     /locus_tag="ACGE36_002278"
     CDS             68..1012
                     /gene="mvk"
                     /locus_tag="ACGE36_002278"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFF8384641.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1005..1982
                     /gene="mvaD"
                     /locus_tag="ACGE36_002279"
     CDS             1005..1982
                     /gene="mvaD"
                     /locus_tag="ACGE36_002279"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFF8384642.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            1995..3080
                     /locus_tag="ACGE36_002280"
     CDS             1995..3080
                     /locus_tag="ACGE36_002280"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFF8384643.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3077..4141
                     /gene="fni"
                     /locus_tag="ACGE36_002281"
     CDS             3077..4141
                     /gene="fni"
                     /locus_tag="ACGE36_002281"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFF8384644.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4390..5193)
                     /locus_tag="ACGE36_002282"
     CDS             complement(4390..5193)
                     /locus_tag="ACGE36_002282"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFF8384645.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5213..5536)
                     /locus_tag="ACGE36_002283"
                     /pseudo
     CDS             complement(5213..5536)
                     /locus_tag="ACGE36_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(5569..5706)
                     /locus_tag="ACGE36_002284"
                     /pseudo
     CDS             complement(5569..5706)
                     /locus_tag="ACGE36_002284"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         1718 a         1233 c         1184 g         1589 t
ORIGIN      
        1 gcgcaactcg ttttgggcgc tttatttttt gcttaaaaat cacttttacg tgagaaggag
       61 ttagattatg gcaaactatg gccaaggaga atcaagcgga aagatcatat tgatgggcga
      121 gcacgcggtt gtttatggag aaccagcgat tgcctttcct ttctatgcaa caaaagtcac
      181 cgcattcctt gaagagctgg atgcaatgga cgatcaactg gtttcttcct actattcagg
      241 aaatttagcc gaagctcctc atgcattaaa aaatatcaaa aaattattca ttcacttaaa
      301 aaaacagcat gacatccaaa aaaacttgca actgaccatt gaaagcacga ttcctgctga
      361 acgtggaatg ggatcaagcg ctgcagtcgc cacagcagtc actcgtgctt tttatgatta
      421 cttagcattt cctttgtctc gtgaaatact attagaaaat gtccagcttt cggaaaaaat
      481 cgcccacggt aatcctagtg gaatcgatgc agccgctact agcagcttgc agccgattta
      541 ttttacaaaa gggcatcctt ttgactactt ttctttgaac atcgatgctt ttttgattgt
      601 cgctgataca ggaatcaaag gacaaacaag agaagccgtc aaagatgtcg ctcacctctt
      661 tgaaacccag cctcatgaaa ctggacaaat gatccaaaaa ttaggatact tgacgaagca
      721 agcaaaacaa gcaatcattg agaattcacc agaaacgttg gcacagacaa tggatgaatc
      781 acagtcactt ctggaaaagc tgacaatcag caatgatttt cttaatctat tgatccaaac
      841 agcaaaagat accggggcat tgggtgccaa attaactggc ggtggacgcg gtggctgtat
      901 gattgcatta gcacaaacaa aaacaaaagc ccaagaaatc agtcaagcac ttgaagatgc
      961 gggtgctgct gaaacttgga tacaaggatt aggagtacat acctatgttt aaaggcaaag
     1021 cacgcgcata taccaacatt gctctaatca aatattgggg taagaaaaat gaagaactca
     1081 tccttccaat gaacaacagc ctttcgttaa cattggatgc attttataca gagacagaag
     1141 tcatcttttc tgatagctat atggtagatg aattttatct agatggcacc ttacaagacg
     1201 aaaaagcaac aaaaaaagtc agtcagtttc ttgacctttt tcgtaaagaa gccggcctct
     1261 cactaaaagc ttcagtaatc agtcagaatt tcgttcctac cgcagctgga ttagcctcct
     1321 ctgccagcgg gctagctgct ttagcaggag cttgcaatac tgctcttaag cttggattag
     1381 acgatctctc tctttcaaga tttgctcgac gcgggtctgg ttcagcttgc cgaagtattt
     1441 tcggtggttt cgttgaatgg gaaaaaggcc atgacgactt aagttcttac gctaagccag
     1501 tcccttccga ttctttcgaa gacgatttag caatggtttt cgttttgatc aacgaccaga
     1561 aaaaagaagt gtccagcaga aatgggatgc gtcgaacagt cgaaacatcc agtttttatc
     1621 aaggctggtt agattccgtt gaaggggatc tatatcaatt gaaacaagca atcaaaacaa
     1681 aagatttcca acttctcgga gaaacgatgg aaagaaacgg actaaaaatg catggaacga
     1741 ctttagctgc ccagcctcca ttcacttact ggtctcctga cagcttaaaa gcgatggatg
     1801 cggtgcggca attgagaaaa aaaggcattc catgctactt tacgatggat gctggcccta
     1861 atgtcaaagt tttagtggaa aacagccatt tatctgaggt acaagaaaca tttaccaaat
     1921 tatttagcaa agaacaagta attactgccc atgctggtcc aggaattgcg attattgaat
     1981 aaagggagat ttttatgatt gaagtatctg caccaggcaa actctatatt gccggagaat
     2041 atgcagttgt tgaaacagga catccagcag ttatcgctgc agtcgatcaa ttcgtaacag
     2101 tcactgtaga atccgcacga aaagtcggaa gtatccaatc tgcacaatat agtgggatgc
     2161 ctgtacgttg gacaagacgc aacggagaat tagttttaga tattcgggaa aatccttttc
     2221 attatatcct tgctgctatt cgtttgactg aaaagtatgc acaagaaaaa aacattcttt
     2281 tatcctttta tgatctgaaa gtaacaagtg agttagacag ttcaaatggc cggaaatatg
     2341 gattgggttc tagcggtgcc gtgaccgttg caacggtcaa agccttgaat gttttttacg
     2401 cgttgaattt atctcaattg gagattttca agattgcagc actagccaat ttagcagttc
     2461 aagataatgg ttcctgcggc gacatcgctg ctagctgtta tggtggctgg atcgctttct
     2521 caaccttcga ccatccttgg ctccaagaac aagaaactca gcattctatc agtgagttac
     2581 ttgccctgga ttggccaggt ctatccattg agccattgat tgctcctgaa gatttacgtt
     2641 tattgattgg ttggacgggt agccctgcct ctacttctga tttggtcgat caagttcacc
     2701 gttcgagaga agacaaaatg gtggcttatc agcttttctt aaaaaacagt acagaatgtg
     2761 tcaacgaaat gatcaaaggg tttaaagaaa ataatgtaac gttgatccaa cagatgattc
     2821 gaaaaaaccg acaattactg catgatttat ctgcaatcac tggggtcgtc atcgaaacgc
     2881 ctgctttgaa caaattgtgt aatttagctg aacagtatga aggagccgca aaatcttctg
     2941 gtgcaggtgg tggcgattgc ggaatcgtaa ttgttgacca gaaatctggc atccttcctt
     3001 taatgagtgc atgggaaaaa gcagaaatca ctccactgcc gttacatgtc tatagcgatc
     3061 aaagaaagga aaaccgatga atcgaaaaga cgaacatgtt tcactagcta aagcctttca
     3121 tgataaacaa aaaaacgaat ttgacttcgt acggatcatc cataaccctt tgccgcaaat
     3181 agcagtttct gatgtcgatc taagtacaca ggcagttggg tttacactaa gcagcccatt
     3241 ttatatcaat gcgatgactg gcggaagcga aaaaacaaaa aaaatcaacc aagatcttgc
     3301 aatcgtcgca agagaagcag atttgatgat tgccactggt tcagtcagcg ctgccttgaa
     3361 agacccttct ttagccgata cttatacgat tatgcgacaa gaatatcctc acgggaaaat
     3421 cattgccaac atcggtgcag ggacttctgt cgaaagagcc caagaagcta tccgattgtt
     3481 tcacgcagat gccttacaga ttcatttgaa tgcacctcaa gagttggtta tgcctgaggg
     3541 agaccgtgat tttaccaatt ggaaagtact tattcaagag actcaaacag ccattgacgt
     3601 ccccctcatc gtaaaagaag tcggttttgg catgacaaga gaaacgctca atgatctagc
     3661 tgctttagga gttcatacag ttgatatcag cggtcgaagc ggaacaagct tcacccagat
     3721 cgaaaacgcc cgtcgctcca agcgggaact gagttattta gctgactggg ggcaatcgac
     3781 agtctcttca ttgcttgaag caaatgaagc agacacttct atggaaatcc tagcctccgg
     3841 aggtatccgc aatgcctatg acatatttaa agccctttgt ctcggcgcaa atgcagtggg
     3901 tacttcagga accgtactga cgcatctgat gaatcacggc gtagaagaaa ctattatact
     3961 gatgaagcaa tggcaagaag agcttcgtct gctttatact atggtcggcg caacgaatac
     4021 cgcggcccta caccaacaat cactgatctt ttccggacca gtgaaggatt ggtgcgaagc
     4081 aagagggatc gacttagtga agtatggaag acgcacagaa aagagcgtgg gacaaaagta
     4141 aagaacactt ttatcccacg ctctaaacac gtataaacgg cggaagcaaa agcaactcct
     4201 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat tccttcttat
     4261 ttcttggagt taaacgcttc tgtcccagcc tcttttttcg aataaatgat gtttaatcag
     4321 ctgtccttcg gtattcgttc aagccgctga tttcacaacc tctccactaa acggtgtact
     4381 aaagctcttt taaggttgtt ttccgatata ggcaagtatg cctccgtcca catacaagac
     4441 atggccgtta acgaaatcag atgcgctaga agccaagaag acagatggtc cagccaaatc
     4501 gattggctcg ccccagcgtg ctgctggcgt acgtccaacg ataaattgat cgaatggatg
     4561 acgttcaccg ttttcttgtg tttcgcgtaa tggagcagtt tgcggtgtag caatataacc
     4621 tggtccaatt ccattacatt ggatattgta ttgaccgtat tcagaagcga tgtttttcgt
     4681 cagcattttt aatccgcctt ttgccgcagc ataagcactg accgtttcgc gtcctaattc
     4741 actcatcatg gagcagatat tgatgatttt accgccgcct ttttcgatca tatcccgaat
     4801 aactgctttt gaaacgatga atggggcatt taaatccacg tcgattactt ggcggaagtc
     4861 tgccgcatcc atttcgatca tcggtgtccg tttgatgatc cctgcattgt tgactagaat
     4921 atcaactgaa cctacatctt ctttgatctg ttgaaccatc gcttgaaccg cttcttcatc
     4981 ggtcacatcg cagacataac cttttgcatc gatgcctgct tctttataat gcgctatacc
     5041 ttcatcaact gattcttgat tcaagttatt aaatacgatt tttgctcctg ctgtagctaa
     5101 agagcgtgca atctcaaatc caataccata gacagcaccg gtgactaatg ctacttttcc
     5161 atctaaacga aacatatcca tactaaattc catcattttc tctcccttat gcttatttta
     5221 attgatccat ttgcaccata tccatgtctg tataggtgat attttctccg cacattgccc
     5281 agatgaatga gtaattgctt gtacctacac ctgaatggat cgaccagctt ggcgaaataa
     5341 tggcttgttc attgtctacg actaaatgtt ttgtttcatc tggtttgccc atcatatgga
     5401 acacacgtgt atcttcattg gcataatcaa aatagacata agcttccata cgacgttcat
     5461 gtgtatgaca aggcatcgta ttccaagcac ttcctggttc taaaatcgta taacccattt
     5521 ataaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca agataccacc
     5581 gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt ttccagcatg
     5641 agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga tgaatgctac
     5701 tagcatgatt cctagcgccc acaa
//
LOCUS       DAXWLH010000150         5723 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXWLH010000150 DAXWLH010000000
VERSION     DAXWLH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.153"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..268)
                     /locus_tag="ACGE36_002285"
     CDS             complement(<1..268)
                     /locus_tag="ACGE36_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8384646.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVID"
     gene            complement(523..843)
                     /locus_tag="ACGE36_002286"
     CDS             complement(523..843)
                     /locus_tag="ACGE36_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384647.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(876..1994)
                     /locus_tag="ACGE36_002287"
     CDS             complement(876..1994)
                     /locus_tag="ACGE36_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384648.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(2023..2817)
                     /locus_tag="ACGE36_002288"
     CDS             complement(2023..2817)
                     /locus_tag="ACGE36_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384649.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(2827..3702)
                     /locus_tag="ACGE36_002289"
     CDS             complement(2827..3702)
                     /locus_tag="ACGE36_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384650.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(3919..4176)
                     /locus_tag="ACGE36_002290"
     CDS             complement(3919..4176)
                     /locus_tag="ACGE36_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384651.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(4179..4403)
                     /locus_tag="ACGE36_002291"
     CDS             complement(4179..4403)
                     /locus_tag="ACGE36_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384652.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(4576..5619)
                     /locus_tag="ACGE36_002292"
     CDS             complement(4576..5619)
                     /locus_tag="ACGE36_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8384653.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         1580 a         1214 c          737 g         2192 t
ORIGIN      
        1 catcaataac tgtagcaata tattgagaga tagaaatatt atcttcactt ctatgtcgct
       61 ctataagatt atatatatca gggctgacat gaaacgtact tctctttttt gtttgccttt
      121 ttctaactgt tacgaccact tttggaactt cactatctcc attgatctct ttgtattttt
      181 caataaataa ttttctagct tgttctcgac tttttgccgg aatttcatct acaattttat
      241 cttctaaata aatatagtgt gttctcatac attcgtcctc tatcttcttt actctcaatc
      301 ttcatgatct tagttcttac cttgtttact cttaaacatt gcaaaaaaat ctagtcaaaa
      361 tcaaacttga aatcatcgtc tatcacaaca cccttaactt catctttaga tttcccaaag
      421 gaacccttat tttcaaatcc tttatttttg aatgatgatg gttatggaaa acttcctttg
      481 aaattctgac aagcataact agtcagaact gttttactta gattaagctt tcaaaagata
      541 ctgatgaaga actaagttct cagtaccttc ccattcttca ataaaaaata tttctccttc
      601 ttttacccac tctatagcta atttcttata atcaaataaa ccatatgtgt ttaagccttt
      661 gtctttcatt aaactcaaaa actgatcccc ttttaatctt tctgtatcat ccatcagaaa
      721 ttcaactaaa tctttatcca tgcgtgcaac tttttcgtct tttagtgcca ttgatttagc
      781 atagtcagtt gaatctttta gaataataac acctacattt ccttccacaa ttttcttctt
      841 catatttacc tcctaccatc ttttccaaag agtatttagt caaaatcaaa ctcgaaatca
      901 tcgtctatca caacaccctt aacttcatct ttagatttcc caaaggagcc cttattttca
      961 aatcctttgt ttttaaattc taaagctttt tcgacttctt ctttggatat tgtatgaact
     1021 tttttcttaa ctactccaag tttatacagc tcttcaaact ctggtacttc ggatctgaaa
     1081 tacaatccat cagtacgaat ttttaatagc ccatatgcgc tatgaacgta tttctgttca
     1141 tatctaacca tacgatctct aaatacgcct gctaccatgg aggaatacgc tcgattatac
     1201 atactgtatt tatcgaaggt gcttgtttct ccttctctta atgatttttg tccgatagca
     1261 ccaaatactt tcgttctaag atttttataa agcttcaacg tatcaggatc agtttttaat
     1321 ggctcgattc tttcaaaata ttctttgagt aatccttttt tcgtcaaaaa tgtgtggcta
     1381 tctaggactt ccatctcttt aatatcataa taatcaagga taagtgttag ctcaatctcc
     1441 gataaccaaa taggctcatt agctgttttg ggattttcgc cataatccgc aaacatcgac
     1501 tgtgattttg gattagcata tgttccttct ttggctgttc cttcaaaatt aaccaaaaag
     1561 aacatatacg tttttaaatt ttcatacggt cttacttttg gtctatttat cgctctcaaa
     1621 atagcttgat ctgtattata ttttgctttc aaataagtgt ttgacggcaa aggataattt
     1681 ctcatgatcc tcgaataggc ttcgttgaaa tcatattcga tcatctttgc tccttcctca
     1741 aacaaatcca agttatacat gctaccggag gtactggctc tataggctga acttggaaca
     1801 aagaaaggtg catcttccgg cttatcatag cccaaaaaat gtattcctgt ttgcttgttc
     1861 tgacggtaaa tgtccatcaa aagatatttg atcgtactaa aagaatctaa ttttcctctt
     1921 gtcatccacc ctaatccgac aagtctttta attttattct gcatttcagg tgtgtatatg
     1981 ctattgtttc ccatttatct gctcatctcc ggctttttta ttctagaccc tatatttgtt
     2041 cattacacca cttatctcgt ctacaaaggc tcccagatcg tgtgtaaagg catcatagta
     2101 atcttcaatc tcactcgatt cagttctccg gtttcttccg tttttatttc tgaatagata
     2161 catctgcgtt tctcttaaat cccttgcttt tttcgcaagt gctaataaat ccgtaaaatt
     2221 atcaataacc ataatgtcat tgatcataaa accttcttca tgaccatact tttcagccat
     2281 ctcgataaaa tcattaacat cgaacaactc caataattct gctttatatt tctctaacaa
     2341 tgtatttaaa tagcgatttc tcaattccca tgaactttca ccagttccgt attcaacctt
     2401 gatccctaaa ttcttagtgt agccttttgc tgtgtatcgt ccaatgaagt ctcccttatt
     2461 ccgattgcta tcttcttctt tgaaaaactc acgccaaatc ccctttttct tcttaggaat
     2521 tagacctctg ctcttgaaat catcatattt ttttgaagct tctcttcgtg tatctaagta
     2581 cctttgcttc agctctgtag ctgattcatt cgttccatat tcaacagtga tccttaagcg
     2641 cctgctataa ccttttgctg tatatcgtcc aacaaagtca ccgccacttc gatcactact
     2701 gacttcctta acaaattctc tccatatacc ttttttgtcc tttggtatta atcctcgtct
     2761 tttcatatct tgatattttt cagaagcttc ttttagagta ttcttttttg ctaccatata
     2821 ttccactcac tctttgttat ctagtatttt aataactggt ttcatttttg taatatcttc
     2881 tttttcagct atatacaact ccgttacggt aacgatctta gtatgattta gattaattac
     2941 tttcacttga tcgccaattt tgatttttcc tcgcaaccgt ttgggaataa accagttgta
     3001 taatcgtgat ttattattat ctacataagc ttttattgca ttttcaggaa acaggttatt
     3061 tagtctttca atttttttac ttttaaaaaa ttcattcgca aacttttcat caacaactaa
     3121 attaaataaa gtttcagaat ttacagcttc catatctatt cctttgtaga taggagaaag
     3181 caatttttta tattttgcca gtaatgactt tccagaatta ctaatcttaa tttggttact
     3241 taaatcttct tgataaagaa ccccatatcg aagtaacgcc ttaatataat gttttaatat
     3301 gccgttgata ccagaataaa tatccatgcc gttctcaata tagtagaaag tgtcgtctct
     3361 ccaaaacaat ttgaagctat tattcaaata aagtaacaag atcaatatat gtttgtcaga
     3421 aattctgaca aatctattac tttttagata ctcaccaatg gcttcactta actctttgaa
     3481 gccattgtat tttcccgata aaaatgtcgt aacattatct tctctctttt tcctatggtg
     3541 tgaacttacc ctgaaataat agagtctatt ctcatttaat attgaaaaat aaatactttc
     3601 agttgtttgt gagcattgta tatagttgaa atgatatcca tctggcatga cttttttaac
     3661 tgtattttgt actagctctg accacctttt gttagtagtc atatattttt ttccattaaa
     3721 ccaaacataa atatcttttt taaaaatttt gttccccccc tattgcgttt tctttgacat
     3781 taaagccctt tgttttcatc acattcgtat agccctagcc actcataacc tgatgctttt
     3841 gcgaagttta tttaaaagtg ttttcctttg taatctacct ttcccctcgt cctggtactt
     3901 ttcttggcga ctattaaact attcaacttt tttagaatca ttgtcagctt gaaatccttc
     3961 acgtttcatt atgtcttctg cttttccaat gctgattcct ttccaatcac gccatcctac
     4021 gcttccctta ttttgacgat aggctttaat agctgattcg gatatttgcc atcttgtagc
     4081 caactctttg gctgtgtact tatttgaaaa aagtagcttc tctatcagtg aaatatcaat
     4141 cattttattc tcactcctga aatcttcatt aatcatctct agctagctat atcttcaatc
     4201 aattctaata tgtcggataa accatcaaca gcttcttcaa gttgatcaac ggattcttct
     4261 gctttttcat atctttctga tccctgtaga tttttaggta tattttcttt gtactcttct
     4321 tcttgaatta cttctaactc atcctttagc tcggaaagtc gctcgcttat ttcttctaac
     4381 ctttttcttc ttctttggtt catcaaaatc acctcttcgc ataatctcta cgattttaat
     4441 ataccacttt tgtgtactac attcaatatt ttttgttata gattcgtatt acaaaaattt
     4501 cctctaaaaa aataagagcc tatttaccat ttcagtaatt aagctcttat ttcatttagt
     4561 tgataattct atcaattaac gtacagcaac cgccattcca gtcacattaa atgctctctc
     4621 atacgtgctt gcattgatcc attttgcgcc aaatacaggg tcaacaacat ggtatgcacc
     4681 atttctgaag ccatccacga gaactacatg tccattccat actgcacgac cccaaggcat
     4741 ttgtttgaac tctggcgtag catagttata agtaatccaa gttacaaccg gattaccatt
     4801 gcgaatttca ttcttgattc cattcgttcc tgaaccagtg atattgacgg cattaccgcc
     4861 aacgtttttt gcccatttaa ctactgggtc agccatcatg ccttgatacg ttccattaac
     4921 gttatgacgc cactctcctg agtaaccatg ataaggatta ttgtcattag cttttggcat
     4981 atagtttaca aaactggtta agctttgatt tgtggctaca cctttgtagt gtaaaccttc
     5041 taataaagaa gctccttcgc ataacattgt atttccttgc catacaacag gaacgttcaa
     5101 cactttttca gtgcttggag caggtgtagg atttggtacg ctgtttggca ttgcatacca
     5161 gcctgtacct tcaatcttcc aaccttgttt actaattacg ttcttttcat tttgatctaa
     5221 cgtgtagtga tgccaattca cgtatttgtt atataaacgt gttacaggaa ctctatttgc
     5281 tccgttgtag gaatagaacg caacaccttc gtacgtccat cctgtgtgtg aaatcttatc
     5341 tctttctccg gcatcaactg tgtaaaaatg ttcaccgtta taccgattat acaaacgata
     5401 aacaggagta ccacttgttg gagcttgcca tgcgacacct tcgtaatgcc atccataacg
     5461 tttcagattg tctctttctc caacgcttct agtgtataga tgctctccgc tattgggatt
     5521 atacatacga tacatggaaa tagagctttc tgctgaaact gaaggtgata ataatactag
     5581 ccccattgtg aatagaattc ctactaaaaa ttttttcatt tgattttacc ccttttcatt
     5641 ttttattgtc attattgcat gaaaattatt cgtaagattg ccaattacat aaacgattgt
     5701 tttaagatca taatcgttgt tgt
//
LOCUS       DAXWLH010000151         5689 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXWLH010000151 DAXWLH010000000
VERSION     DAXWLH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.154"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(488..1297)
                     /locus_tag="ACGE36_002293"
     CDS             complement(488..1297)
                     /locus_tag="ACGE36_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8384654.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACGE36_002294"
     CDS             complement(1297..2043)
                     /locus_tag="ACGE36_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8384655.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACGE36_002295"
     CDS             complement(2061..2528)
                     /locus_tag="ACGE36_002295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8384656.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACGE36_002296"
     CDS             complement(2528..2938)
                     /locus_tag="ACGE36_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384657.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACGE36_002297"
     CDS             complement(2949..3962)
                     /locus_tag="ACGE36_002297"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8384658.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACGE36_002298"
     CDS             4179..4913
                     /locus_tag="ACGE36_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8384659.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACGE36_002299"
     CDS             4897..5607
                     /locus_tag="ACGE36_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8384660.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAXWLH010000152         5534 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXWLH010000152 DAXWLH010000000
VERSION     DAXWLH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.156"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            136..465
                     /locus_tag="ACGE36_002300"
     CDS             136..465
                     /locus_tag="ACGE36_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8384661.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(752..1237)
                     /gene="vanZ-A"
                     /locus_tag="ACGE36_002301"
     CDS             complement(752..1237)
                     /gene="vanZ-A"
                     /locus_tag="ACGE36_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8384662.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1390..2301)
                     /gene="vanY"
                     /locus_tag="ACGE36_002302"
     CDS             complement(1390..2301)
                     /gene="vanY"
                     /locus_tag="ACGE36_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8384663.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(2729..3337)
                     /locus_tag="ACGE36_002303"
     CDS             complement(2729..3337)
                     /locus_tag="ACGE36_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8384664.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(3343..4374)
                     /gene="vanA"
                     /locus_tag="ACGE36_002304"
     CDS             complement(3343..4374)
                     /gene="vanA"
                     /locus_tag="ACGE36_002304"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8384665.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(4367..5335)
                     /locus_tag="ACGE36_002305"
     CDS             complement(4367..5335)
                     /locus_tag="ACGE36_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8384666.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         1626 a         1193 c          956 g         1759 t
ORIGIN      
        1 acgcacttgc aagtaagttt acttcaatac atgaaattgc ttataatagt gtattggaat
       61 taaggtagat tgacaaaaga gtgatcaaat aggtatactt cattcaaata atcgtttgaa
      121 tgtgaggtga gatgaatgaa aaaagtttgc caagtgactg tcgtacataa agaaaaagta
      181 aatggaataa aaaataaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
      241 tgtttcagcg atttttctag aattaaaatt ttctatgctt tagaaaccta taaggaaatg
      301 tgcgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
      361 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattac
      421 tctttagcga atgaagacat cctttccgct attcgtgttt tttaaagtta tcagaaaatc
      481 tatctatgaa atcttagttt gggggtaccg tcaggaaaat gcggatttac aacgttaagg
      541 aaatttatta aagagaccaa ttcctcagca ctaaaattga taattgggct ttctttgtta
      601 ctatagtgtt aggtaagctt tttactttat caccggtttt cccctcactt cacacctacg
      661 ggcgagtttc cccacatccc gttttccatc taatccaatt cattcaatat ctttatgtgt
      721 atgaaagatc agattgcttg atttagacat cttacacgta atttattctt aaatgggtac
      781 ggtaaacgag caataatacg agcaaaagta tacctacaaa aataataact ctgtctaatt
      841 ttttttgatt catatgctta ttgcttaaac catataattt cagtccaaga aagcctccaa
      901 cagtatttgt aattacatct gttatgtctg tcgctccaat agcgaagata aattgaatta
      961 tttcaaaagt aagacttaaa accagtacaa aagcaaactt aggtaaaaat ccgatttctt
     1021 taaaattgac attcaaaagc aagccaaatg gaataaagat tataacatta tctatcatct
     1081 ctctgaaatt cccagtagca gtaaatggag tcaagttaag acttctttga tgataattaa
     1141 atactgataa aatattgtat tgtaatttga ataacactaa ccagattagt gtcactaaat
     1201 ataaagctag caatcctcta gataatattt ttcccaatat aatttcctct aattcttttg
     1261 tttaagttgt atcattagta tagcaatgtc ctagttaacg tgggttttaa attaaaaagc
     1321 taacgttacc ccaaaagtgc tagtacaata atgctgttca ttaatttcgt tggtttcatt
     1381 ccgccatcct tacctccttg aattagtatg tgttgatccg ggaaacactg tcacaattac
     1441 accgtctata ttgtttcctg atatctcata acgaagatta gtcggcacat gaatggtggt
     1501 atttttagta acaggataat aaaagatctc atatttttcc ccatttacac taacagaaat
     1561 ggttttttct tcttttaggt aatccatata ttcctcgaga acgaaattct tttctttcat
     1621 aatcgcacta tgtggtaaac caacatagcg aatatgccat agttcatatt gaattcctgt
     1681 taactctgtt ttgtcctctg gataacgtaa aatgaacccg tatttccaag cattttcttc
     1741 tatccacttt ccttcagggg ctcgttccat tttcgtcaag cttgatccta catctagtga
     1801 taaacctgaa ttatgctcac tataacctgc tggtaaggca tactcagccc ccatttcttg
     1861 gtaaagcaca ctttgctcat caaagtctcg atagccacta ttaataataa aatgactaac
     1921 gccacccttt acagcatcat tgaccatctc tgaaaatttt tgtgctattt cttttgacat
     1981 ataaatatta ctatcaagca acccgtatcc atttattaat tcgtcatgtt tagataaatt
     2041 cacgatatct gacttcacac tttcttggcg aacaggatat ttactattga ttaatagcag
     2101 atttccttga taaacctgtt cttctgtaat tgttttctct tgggtatttt cagaagtccc
     2161 actattttct aaatgttctt tgggattttg atcataattt tgaaattcga ctttttcctg
     2221 agaaaacagt gcttcattaa cgtagtcata acctaagtat attaagaata acaataacaa
     2281 taaaaaaaac aacttcttca ttttcagtct cctccttaac tatatagttg gaagtatgaa
     2341 atatttctag aatcttcccc ttaaacggca caatctcgtt tgagattgtg ccgttttgcc
     2401 ccgtaaatta cagtcaatta tttcgcccta ctggatttac tattatcacc aatgtagatg
     2461 aattttcata agtgataccc atgctgaatt taacgaggct gaactgcctg ttgacaatgg
     2521 gcggtgtgaa ataccacccc tctatgcgat cagtcttatt atcaggctat cttgccgcag
     2581 cgcacgcaga taatgaccgc aatatcgctc tgccttacat cttcaatact atctactata
     2641 tataaattcc ctaagaacca gttacatacg tcgggtttcc tgccgaaaga gttagcttat
     2701 atagtttgtc cgtgcaacgg ttaaaagttt atttaacggg gaaatcaaaa tagctattgg
     2761 ggtatggttc gtctcttaat acatagtgcc accattcgag gctatatgct tcaaacccac
     2821 tgttttccat gatggagcgc aaacgtctgc gattttgcgc ttcattgcat gatattccat
     2881 ttgccgcatg atgagagcgt tcatccataa aatcaaatcg gctccccatt ggtacaagct
     2941 cacccgtgtc taatcgataa agcgtaagat caatggcact gccgcggcta tggcttgatt
     3001 ttgaagccac gtatcctttt gaaatcatct cagttcggtc aatattggga taataacttt
     3061 cctttgtcag gttattttcc ggctgtgcag cccattgcat aaaacagttt acagcacgat
     3121 taggacggta accgtcccat agaagcaatc cgtacccttg ggtagcagcc agttcttttg
     3181 ccttcaaaag cgattcagcc aactcgtatg tccctacaat gcgatttact tcataaccgt
     3241 caaccggttt tccggtgaaa ttatcccaag tggcatattt agcgtcccaa cgaacaccgt
     3301 gtactatttc atctaaaaaa gtaaatccta tttccatgct tatcacccct ttaacgctaa
     3361 tacgatcaag cggtcaatca gttcgggaag tgcaatacct gcagcggcca tcatacgggg
     3421 ataacgactg tatgacgtga aaccgggcag agtattgact tcgttcagta caatgcggcc
     3481 gttatcttgt aaaaacatat ccacacgggc tagacctcta cagccgagcg ctttatatat
     3541 tttttttgcc gtttcctgta tccgtcctcg ctcctctgct gaaaggtctg cgggaacggt
     3601 tataactgcg ttttcagagc ctttttccgg ctcgacttcc tgatgaatac gaaagattcc
     3661 gtactgcagc ctgatttggt ccacctcgcc aacagctaac gcggcactgt ttcccaatac
     3721 cgcacaaccg acctcacagc ccgaaacagc ctgctcaatt aagattttgc tgtcatattg
     3781 tcttgccgat tcaattgcgt agtccaattc gtccgcgcta ttgacttttt tcacaccgaa
     3841 ggatgagcct gaacgcgccg gcttaacaaa aacaggatag gtaaacgtag ctgccaccgg
     3901 cctatcatct ttattaataa cccaaaaggc gggagtagct atcccagcat ttttcgcaac
     3961 gatgtatgtc aacgatttgt ccatacaaat tgctgagctt tgaatatcgc agcctacaaa
     4021 agggataccg gacaattcaa acagaccttg tatggatcca tcttcacctg acttgccatg
     4081 caaagctgaa aatgctacat caacatggtt gatttcatat tcatggttct ttttaacaag
     4141 taatccgtgc atttttttat ccggcgagag tacagctgaa tagcaattgt cgttttccca
     4201 ttccgcgcaa ggtttttcgc acattttcca tacaccagat ttcgtaattc caatgtataa
     4261 cggctcgtat ttttctttat taatgttagc ggctatctct attgcagatt ttaccgatac
     4321 gtcatgctcc tctgagcaac ccccaaacag tattgcaact tttattctat tcatgctcct
     4381 gtctcctttc aaaatccaaa cagtttttaa tggttttttc aacggtatca cgcaacgctt
     4441 gctcggtata ataggccgta tgcggtgtga ttatcacgtt aggcattctt tgaagtttaa
     4501 gtaaaaattg attatcaatt ggtttttggg tgcaatcaga gtagaaaaac tcttcctctc
     4561 cttccaatac atccaatgcg gcaccgccca gtttcccgtt ttctaatgct ttaaccaact
     4621 cataggtatc tacaagtgga ccgcgcccag tattgataag aaatgctcct tgcttcattc
     4681 tctgtatttg ttcgtggctg ataatatagt gcgtatccgt attgagcggc acatgaagcg
     4741 taacgatatc gctattttgc agcaactcat caaacggtac atagtttacc tctatacttc
     4801 ggctgcgact ataagccaac actttacatc caaatcctcg cagccgctca ataaccgctt
     4861 tgcctatctg gcccgttccc accacaccaa ctgtcatgtc gctgagtacc ttgccacggt
     4921 cgctgtccaa cctgaaatca tgtttttcca cagagcgcac aatcgatttt acgttgcgta
     4981 ctgccataag aattagcatc atagtataat cggcaacgct atccggcgag tacgccacat
     5041 tgtcgacagt gatgcccatt ctcttagcag cagttgtatc tatatgattg cagccgatgc
     5101 ttcgggtaga aatatatttc acaccggctc tcttcagcgc aagaagaata gaggcggaaa
     5161 tctctgattt atgtcccaca ctgatacatt gattgaaagg cgcggatttg gcgttggatt
     5221 ccgacacgtt ggcgttaatt atcgttgcca taacgccaaa gcgaggcgaa agagcatgga
     5281 atgcatctgc ctcatcctgc tcacatccat aaacagtaat gccgatgtta ttcataatta
     5341 agaccaaccc tttctgtgaa aggcaccaaa taaaacgggg atgccaatgg taatcttgtc
     5401 aaatctttct ctgaagaacg aaaacggctc gttcttacgg tattaagtct accgtgtaag
     5461 aacgagccgt ttcttaatat taaattgtta atttcctaag aaaaagtaaa gataaccttg
     5521 agattttcct aaaa
//
LOCUS       DAXWLH010000153         5336 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXWLH010000153 DAXWLH010000000
VERSION     DAXWLH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.157"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(202..1137)
                     /gene="whiA"
                     /locus_tag="ACGE36_002306"
     CDS             complement(202..1137)
                     /gene="whiA"
                     /locus_tag="ACGE36_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFF8384667.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1171..2169)
                     /gene="yvcK"
                     /locus_tag="ACGE36_002307"
     CDS             complement(1171..2169)
                     /gene="yvcK"
                     /locus_tag="ACGE36_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFF8384668.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2166..3050)
                     /gene="rapZ"
                     /locus_tag="ACGE36_002308"
     CDS             complement(2166..3050)
                     /gene="rapZ"
                     /locus_tag="ACGE36_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFF8384669.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3185..3916)
                     /locus_tag="ACGE36_002309"
     CDS             complement(3185..3916)
                     /locus_tag="ACGE36_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFF8384670.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(3920..5185)
                     /locus_tag="ACGE36_002310"
     CDS             complement(3920..5185)
                     /locus_tag="ACGE36_002310"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFF8384671.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         1509 a         1156 c          888 g         1783 t
ORIGIN      
        1 atttatgtac aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
       61 taaaaacaat agaagccttt ccaaattatg ttactccagt aaagatggaa ttatagtttg
      121 acggaagaaa agcaagtata acttttaaaa aaaactgata cattcgcatc tcctgcttca
      181 tgtatcagtt ttttctttgg attaaactgc ttcttcacgc agtttttccg caaattcatt
      241 tattttgcga attcggtgat taattcccga ttttgatatc gcacctgagg gaatcatttc
      301 ccctaattct ttcaagctga cctctggatg ttgaagccga agttctgcga tttcctgaag
      361 tttttcagga agggcatgta gaccgacctt tgcttcgatc aattcgatgt tttcgatttg
      421 tttagaagcc gcatcaatcg tcttgttcag atttgctgtc tcacaattga ctagacgatt
      481 gaccgaattt ctcatatccc gtacgattcg aacatcttca aatcggagca ttgagttggt
      541 tgcaccaatc aatgttaaga aatcagcaat tttttctgct ccttttaaat aagaaatata
      601 gccattgcgc cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca tatcgcaaat
      661 gtcttggtta tgctgttcat aaatagaaaa tatttccaaa tgataacggc tcgtttccgg
      721 attattgact gaacctgagg ccataaaagc accacgtaag taagaacgca ttttttgcga
      781 atttcccata atctcgtccg aaacgtgtcc attgaacatc ataccatcca tgatatcaag
      841 atccatcaac acatcttttg tgccttgctt caaccggacg atatagacat tgttcttttt
      901 taatttcatt tttttacgaa cgagtagctc acttcgtacc tgataatgat ctttcaaaag
      961 agaatagata cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa taaactgctg
     1021 gttcacaata cctaaagagc cgttcattcg cagcaaagca gctaattccg ctttcgcatg
     1081 ttctcgatga acttccaaac cagttagttc tttcttcaca tctgcagcaa atgacatcct
     1141 attctcctcc tttctccaac aataaattgc ttaatatttt gaaccgaaaa ctaaacggaa
     1201 caattcttct actactttgt ctccatcgtg gaagacacca ccatctttca attccaaaaa
     1261 gtcggtggaa atcactcgac atccttcatt tctcaatccg ttgaagtcgt gtgcgacttg
     1321 tactagatac tcatcataga tctctgggtc catataatct tcaggcactt tttccgtatt
     1381 gaccaatact gtatcgataa aaggctgatt caaatgcttg tgtaaaacac gtatatgatc
     1441 ggcatctgtg aaatgttccg tttccccttt ttgggtcatg atattacaga tatagacggt
     1501 ttcagcagtt gtcttgagca ttgcttcgcc gatttctggt atcactaaat ttggaaggat
     1561 actggtgaat aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa ttgctgaaac
     1621 gacttttcga gccgctttgg cttcactttt atcatttgtg ttgcagacat acacacggtc
     1681 aatggtttta cggtcgatcg cgattttaga ttctcctact gcagtcgttc cgtctttaaa
     1741 gacagcatgc aaggtcaaag gagtttctga agaaggataa acatgtccat ctacatgcat
     1801 catttttgct aataattgaa tcgcctcata agtgcttccc cgcatctcgg aaactgcggc
     1861 aatgatcaga ttaccgatcg tatgattggc taaaaactga tcttcctttt tgaaacggta
     1921 ttggaaaata tcttcataga attgtggcat atccgataac gccactaaga catttctcaa
     1981 gtcaccagga ggagccatag cgatcgattc tctgattgca ccgctgcttc ccccatcatc
     2041 tgcaacagtc actactgctg tgatatctgc tccttgattt cgcaaacttt tcaggataac
     2101 tggcagacca gtgccaccgc cgaccactac gatctttggt tttcttatcc ggtaggtttt
     2161 cattttcacg accggttcac cgtctctttc cttttatcct tgtcccgatg tgtcacattg
     2221 actttatagt ttttccctaa agcatttgcc aaacgttcag tcaatgccac agaacggtgt
     2281 tgaccgcctg tacagccgat tgcaactgtc aagctgcttt ttccttcttt aatgtatcca
     2341 ggcaatacag tttccagaag atccaaaaat ttatggtaga atgattgcgt ttcatcaaag
     2401 gacatcacat aatcatagac tggctgatct tttccggtca atggtcttag ttccgggata
     2461 taatgagggt tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat cggcaacccg
     2521 tatttaaaac caaatgatac aaattcgatc cgaaatcctt ggtctccctt tgatttgaat
     2581 gactcattga ttttttcacg taattgtcgt ggtgtcaatg tcgtcgtatc aatgatcacg
     2641 gaagcttggg ctttcaagtc gtctagtatc gcccgttctt tacgtatccc ttcagtgatc
     2701 aaaccgtcca tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc cactagttct
     2761 tcgtctgaag catctaaaaa taagatagaa gtgtcgatga agttcgtatt ttcgatttcg
     2821 atcagcatgt tttggatttc ttcaaagaag gaacgagaac gtaaatccac tcctaaagcc
     2881 atttttgtga ctttgcctga ttctttgatt aattcccaga attttggaat caatcgaggc
     2941 ggcatattat cgatacaaaa atagcccata tcttcaaaac tttggatagc tactgttttc
     3001 cctgcaccgc tcatacctgt tatgatgact aattgcaaat tatctgtcat aattacgaac
     3061 tcctctcaca ttgtgcatag taaaattata tcattccggg cgtatcatta ccagttttct
     3121 ttttctttta caaaaagatt tggaccaaat atcttttagg atatttgccc caaatccaat
     3181 ttttttactt ttccgatgct gtatccaatg ccttgcttct ttcagctagc gctcgttcaa
     3241 tcgtccgatc tgctaaaata gattgggcat cgatcactgg ataatggtta tcttcagcaa
     3301 attcatgcat caatgacaat tctgtgcaac ctaagataac tttttcacag tttaggcgct
     3361 ctaccgcttc ttctaagatt tcgtaatata gctcttgatt caaataatcc gattccttga
     3421 tctcatgata gatcagataa ttgatttttt cttgaagaac agtatcagga atcactgttt
     3481 cgaatccaag atttttgact tcgcgttcat agattccagc tttcatactt ccttctgttc
     3541 ctagaatcgc tactctccct gtcgtatgct gacggacaag ttcatttgct gcttctcttg
     3601 gcatatgaag gataggaata tccgttgccg cttgtaattc ttcaaaaaag tagtgtgcgg
     3661 tgttacaagt caacacaatg aagttcggct gcaataaatt ttgtttctca atatcatcca
     3721 gcagaaacgg cattggattt tcttctgatc gatctaaaat ataagctgta cgatcaggaa
     3781 ctgttgcatg attgaataag acataattca aatattcttg atcttttgta gctttcgttc
     3841 ggtggttgat caagcgaaca aaactttctg tcgccatcgt gcccattccg cctaaaatac
     3901 tgaaaaaatt ctccatgaac tagccttctt tcacatgaaa atactttttg aatcttggaa
     3961 tatagttatg gaacatatat ttcatcaaca accagcgctt gattgaccgg tctttttcat
     4021 aaaacacagt agtaccgtaa cgattttctt tgatcatttg ctcagctagt tttttgtctt
     4081 cattttcccg agcatattct aaaaagattt ttttgggcac acccatccat aatcttgctt
     4141 tatctgattg attcgttcca taggtcgttt caacaaaggg tttgttaaac actcgatctt
     4201 ctgtgacgaa acgagccaga ttcaagccgt tcaatgtcac aaagaaactg ctacgacctt
     4261 gtctcaaatt gatttcaaat aatttatatt ctccatctct aggatcatac ttcatatcga
     4321 aattggcaaa gcctgtatat tcgatttttt ccaaaaatgc cttaatggtt tgatagattt
     4381 tttcgttata atctggcatg atcacaacgt aattaccaat cgatgctggt gtaggatctt
     4441 ctaacagtgg atgaccaaga cacatcatcc gaacttgatg atcttcatct acatacgcat
     4501 ttagcacacg catattacta tcatcacccg ggataaaatc ttgtacaatc atttcacttt
     4561 tataaccggc ttcgtatatc cggcctaaaa ttaaatcgaa ctcttctcta gtctctaaaa
     4621 taaaagcttt ttttcttcct tcaaattgga cagaaagata ctccacactg tttgcaggtt
     4681 tcaaagcaac tgggaattca aaaggcaatt cctgttccaa atgtccattt actaacattt
     4741 cttctctgac gatcaatgtt ttaggatacg gaagatcata ttcttcacaa acttcataaa
     4801 agcttacttt attgatcagt ctttcaaata aagaataatc aatatatgga cagataaaat
     4861 attctgacag ctcttgttta tgttgggaga tcaattctgc atatccatca ccacaagcaa
     4921 tcaaaagata ttttttactc ttatcagaat atctttcttt tgccaaacga agcatcgtct
     4981 cgataaacac agggtcttta tcaaatcctg gaataacttc tacattgaca attttactgt
     5041 agcgggtagg ggctaactga tccgaagcat atgcttggca gattttgccg tatgcttcat
     5101 taaaagaacg tgccatacca taaacgttca tgtcgcttcc taataatata gggataaatt
     5161 cttcattttc aatactgttc atcattatct ctcacttact cataatctct tgtttttctt
     5221 ttccaactac acaaggaata tcataccata aagaaaaaag caatcaaagg aaactcctcc
     5281 gattgctcaa ttcttttttg aacggattga acttatagga agtttttgtc tatacc
//
LOCUS       DAXWLH010000154         5310 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXWLH010000154 DAXWLH010000000
VERSION     DAXWLH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.158"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(60..1199)
                     /locus_tag="ACGE36_002311"
     CDS             complement(60..1199)
                     /locus_tag="ACGE36_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8384672.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1508..2221)
                     /locus_tag="ACGE36_002312"
     CDS             complement(1508..2221)
                     /locus_tag="ACGE36_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFF8384673.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2475..3176)
                     /gene="nagB"
                     /locus_tag="ACGE36_002313"
     CDS             complement(2475..3176)
                     /gene="nagB"
                     /locus_tag="ACGE36_002313"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFF8384674.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3410..4942
                     /locus_tag="ACGE36_002314"
                     /pseudo
     CDS             3410..4942
                     /locus_tag="ACGE36_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(5133..>5310)
                     /locus_tag="ACGE36_002315"
     CDS             complement(5133..>5310)
                     /locus_tag="ACGE36_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8384675.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1748 a         1079 c          892 g         1591 t
ORIGIN      
        1 aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct tttttatgct
       61 tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac atgttccatt
      121 cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac tgcatagaca
      181 ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat caataccaag
      241 atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt gatcaaattt
      301 ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg aaatggagag
      361 ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac atacgggata
      421 atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc gaatattctc
      481 attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact gctgatatag
      541 gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg aatcgctttc
      601 ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat aaatagcaaa
      661 ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat gatttttgag
      721 acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga taaatttgct
      781 gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc caattcattt
      841 aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg aattgtctgt
      901 tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat tacgaccaaa
      961 aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa aacaacgatt
     1021 ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaat tgccgaaagc
     1081 attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac gccgatgatc
     1141 gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg tgattgcata
     1201 catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag ttacgcgctg
     1261 agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga gtagacgagt
     1321 acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc cctttttcca
     1381 aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg aaaaatataa
     1441 ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt ttacaacctc
     1501 agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg tgtacggaca
     1561 actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta tagatatttt
     1621 tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg attacgatat
     1681 ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg ataaattgga
     1741 tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg agtgcaatag
     1801 caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg gcgatcccta
     1861 atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat tgtttcgtga
     1921 cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca atcagccttg
     1981 cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct cttaatgcac
     2041 cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa actaaaatat
     2101 gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt gctccgccaa
     2161 atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc atatcattca
     2221 tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa aaagtgcaat
     2281 ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct ttttatgaat
     2341 aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa tatggggagc
     2401 tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca acctctttag
     2461 cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac aacaacattg
     2521 tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc aatcatccct
     2581 ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt acttttcata
     2641 attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt atcgaagaat
     2701 cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt tccgtcaaat
     2761 gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa gatttggata
     2821 tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc cagatcttct
     2881 gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa ttggacatcc
     2941 atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc atctaagttt
     3001 acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata taaagtaact
     3061 ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc ttctttgatg
     3121 atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat ttccatagtg
     3181 attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct ttttacaata
     3241 attagtatag accaaattgc atttttttca ttttcttgac aataaatagg aatccgtgtt
     3301 aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa gcaagtgatt
     3361 cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta tgagtatact
     3421 gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag gtgtcgatct
     3481 gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg gtaaaagtac
     3541 attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc attggcaaaa
     3601 aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat taacaatcga
     3661 agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa ttgcttttct
     3721 ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg ggaaactcca
     3781 aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg agatgagcag
     3841 cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa gtggtggaca
     3901 acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc tgatcttaga
     3961 tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt ttcttcagga
     4021 ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc agatcaccac
     4081 ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc taaaacaagc
     4141 attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg ctcaacaaaa
     4201 acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc gatcaacgat
     4261 ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc cagcttcagc
     4321 aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag cagtggaaac
     4381 aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt tgaccatccg
     4441 ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta ctttattaaa
     4501 aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag ataacacaaa
     4561 aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac cactacaata
     4621 tttaagcagt taatttccaa aagcgactat caaagaatta cgtcgccaac tagctaaagc
     4681 agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg gggaacaaac
     4741 taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc tcgatgaacc
     4801 gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac agaaatttcc
     4861 aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg tgcaacgagt
     4921 cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg agctgtgaca
     4981 aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg accttcggta
     5041 ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc ttattacttg
     5101 atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag atttctcttt
     5161 cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag cccgctaacc
     5221 gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga accagccgca
     5281 cctccaataa agtacataaa cataaataca
//
LOCUS       DAXWLH010000155         5289 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXWLH010000155 DAXWLH010000000
VERSION     DAXWLH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.159"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            191..1294
                     /locus_tag="ACGE36_002316"
     CDS             191..1294
                     /locus_tag="ACGE36_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8384676.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(1291..1497)
                     /locus_tag="ACGE36_002317"
     CDS             complement(1291..1497)
                     /locus_tag="ACGE36_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384677.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1487..2554)
                     /gene="rlmN"
                     /locus_tag="ACGE36_002318"
     CDS             complement(1487..2554)
                     /gene="rlmN"
                     /locus_tag="ACGE36_002318"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFF8384678.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(2784..5231)
                     /locus_tag="ACGE36_002319"
     CDS             complement(2784..5231)
                     /locus_tag="ACGE36_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8384679.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         1454 a         1155 c          937 g         1743 t
ORIGIN      
        1 ttattgtcgt tttcaatgtt tttgtacttg ttcgttttta ggatacttat tgatgatttc
       61 aaggtgttca tgccacctta ttcatcactt atcctgtctt aggttcacct ctactccatc
      121 tacagccgca gtcagatcga tcactcttta aaattttaac ccgtttcaac gaagaaagaa
      181 ggaaatgaat atgagtcaaa gtgttttctt accaaattat accattggaa cagatgcgta
      241 tgaaaaagca aatggaatcg tcaaagaata tgggacaaaa gtagtgctga ttggagggaa
      301 gactgcactt tcaaaggcag aaaacaaaat aaaagtagct ttgacacagg aaatagaggt
      361 tgtcgccagt ctctggtacg ggggtaattc aacttatgga aatattgaac agttgatcca
      421 gcatgaaagt gtccaaaaag cagacgtcct gtttgcagtc ggtggtggcc gtgcttgcga
      481 tactgtaaaa gtcattggtg aaaaactagc taaaccagtc ttcacctttc ccactattgg
      541 ttcgaactgt tcgcccgtca ctgcggtatg tgtcatctat aacgaagaag gcgtaatgga
      601 cggactcttc tttccttcaa aaccacctgt acacaccttc attgatacag caattattgc
      661 cgaagcacct gataaatact tatgggcagg tattggcgat gctttatcca aagaagtcga
      721 atctactttt tctgctcgtg gcgatgatct aggctacacg gatagattag gaatccaaat
      781 cggaaaaatg tgcattgatc gtctcttgac ttatggtacg caagcattga aagatagtgc
      841 cgacgatcgt ccctcatatg caattgaaca agtcgtttcg gatatcattg ttactaccgg
      901 attggtttct gttttagtta acaataacta taattctgct atggcacact cattctatta
      961 cggtacgact gttctagaaa aaatggaggg ataccttcac ggagcatctg tcagttatgg
     1021 tgtactgacc ttattgatga tggatcagca gttcgatttg tttgataaaa tctatgtgtt
     1081 tatgaaaaag aaccaacttc cagtatcgtt aaccgacatg aatctcaatg tcacgaccga
     1141 tttagaagca atcttagata aagccatggc aacaaatgat attaaacatg tgccttatga
     1201 aatcacccga gaaaaattta aacaggccat tcttgacgta gaaacttatc atgctacaca
     1261 agaaagccag caaggatata agaaaatcag ttaaataatt gctaaaaccc ccatcaagca
     1321 ggctgtcatc aaattgagga catttctttg cttgatgagg gtttttcttg tcaaaaaaag
     1381 ctttatagct ttcgctatag aatcttttct cttatttaag ttgaacagtt ccttaattaa
     1441 gaaaactgct ccttttatgg taattgaaaa aacgagtagg atacgattat ttggcaactg
     1501 tttttttcat ttgcttgctt cgcaattgtc cgcaagctgc atcaatatct gtaccatgtt
     1561 ctttacgtat cacacagttt acgccgtttt tcttcaatac atcataaaat ctcaatacat
     1621 cagcttttgg actgcgagaa tattgatcat gttcactgac tggattatat ggaatcaagt
     1681 tgacatatgc taattttttc ttatctttta atagatctgc taattgttgc gcatgctctg
     1741 gtctatcatt tacttgattc aacatgatat attcaaacgt gattcgacgg tttgttttct
     1801 ctaaatactc atccacagct tccatcaatt tctcaattgg gaaacttcgg ttgatccgca
     1861 tgatcgatgt tcgcacctca ttattcggcg cgtgcaagga aatagccaga ttgacttgca
     1921 agccgttatt agcgaattcc ttgattttgt gtgctaatcc actagtggaa actgtaatgt
     1981 gccgagcacc aattgccaat cctttggcat cattgatgat atgcaaaaag tccattacat
     2041 tgtcgtaatt atcaaatggc tcaccgatcc ccattacaac gacatgtgaa acgcgttcgc
     2101 caagattgcg ctcatcaaaa taatgttgga ccatcatgat ctgtgcaaca atttctccag
     2161 cagtcagatc acggttcttt ttgagcagcc cgcttgcaca aaacgtacag ccgatattac
     2221 atcctacttg tgtcgtcaca caaactgaca agccatattc ttgtctcatc agtacagtct
     2281 caatcatgtt tttatcaggg agttcaaaca aatattttac tgtaccatcc gaagcttctt
     2341 ggacgaccat ctgttttaga ggattgatga taaaatgctc cgataatttt tcgatcaatt
     2401 gtttcgatag attcgtcatt tctgtgaaat tacttacacg tttttgatac aaccattccc
     2461 aaacttgggc agctcggaat tttttctcgc cgttttctat aaaccaattg attaattctt
     2521 cattcgtcaa gccataaatg gactgttttt ccaaagtatt ttccctcttt ttctcaaagt
     2581 ttcgctacat actatacatg attcatttcc ttaatgcaat acttcacaaa taaatcgaat
     2641 agaactttaa tctcacaaaa actaaaaaga caagacatca taccgtatta gaaaagatgg
     2701 aaaaaaacca ttttttcttt tggtatcatc atgccttgtc tttttattgg ttattcgttt
     2761 ttcaaccctt catcacacaa gttttacatg tcattatcgt ccggcaacgt ttctgatgtt
     2821 tcttcctgct caatgaatac tttccgaggc ttgcttcctt ctgacgggcc aacaactcca
     2881 tgagcttcca gttcatctac taaacgtgct gctcggttgt aaccgatccg aaatcttctt
     2941 tggagaaggg aaatacttgc cgtttgcatt tcaacgacaa gtgcttttgc ttcttcaaac
     3001 agctcatctt gtggctgttc tggagcacct gtcgtttcaa cttcatctgt cggcatcatt
     3061 ttctcttcat aatgagcttc ttgctgatct gtaacaaatt gaacgacccg ttctacttcg
     3121 tggtcagaaa tgaatgctcc ttgcacacgg atcggtttat tctcacccat tggcaagaat
     3181 agcatatctc ctcgccctaa gagcttttct gccccattag aatcaatgat cgtccgagaa
     3241 tctgtaccgc ttgataccgc aaatgccatt ctggaaggta catttgcttt gatgattcct
     3301 gtgataacat ccacacttgg acgttgagta gctaggatca tatgaatccc cgctgcccga
     3361 gccatttgcg ctaaacgaat aatcgcatct tcaacttcat tgctggctac catcatcaaa
     3421 tcagctaatt catctacgat cactacgatg aatggtaagg ttggatgttt cgttccgttt
     3481 tccaagtttt tctgttgaac aaattcattg taaccagaaa tatttcttac tccggttgcc
     3541 gcgaattttt catatcgttc ttccatttct tgtactactt tttgtaaggc ttgcgctgct
     3601 tttctagggt tcgtcacgac cggcgtcaac agatgaggta tgccgttgta catattcaat
     3661 tcaaccattt tcggatcgat catcatcagt tttacttcat ggggcttagc acgcattaat
     3721 atacttgtga taatcccatt aattgctact gacttcccac taccagtcga accagccacg
     3781 agtaagtgag gcatcttcga aagatcagcc gtttgtaccc gtccagaaac atcacgtcct
     3841 aaaggtactt ctagtaattt atctgggtgg tttggttgtg cttcgatgat ttctctaaag
     3901 gaaaccatac tgatctttcc atttggtact tcaataccaa tcagtgattt acctggaatc
     3961 ggtgcttcca tccggacatc tttcgccgct aaagcaagcg cgatatcatc tgttaagttc
     4021 acaattttac ttacttttac ccctacagcc ggctggactt cgaattttgt gacggatggt
     4081 cctaagctgg ctttgacgac cttggcatcg acaccgaaac tttggaaagt ttgttctaat
     4141 acgccgatat ttttttcaat ttttttatac tcatcacttt gatccacagt tggtatagaa
     4201 tcaagcaaat caactgtcgg taattcatag tcttgatttt cctgttctgc ttcaatctca
     4261 aattctaatg aggtaccgtc atcctctggc aactcatctg aaaaaccttc tgctgtattg
     4321 gtctgttttg gtgtatctgg tactggtttc tctagattcg caggatggat attttcttga
     4381 aaactgtcga ttggaacaaa acttagctgc tccggctctg tattttctgg ttcctgccat
     4441 tcatcagcag tattttccct ttgctggctc accgcttttt tcttctcata ttctgcagct
     4501 tctttttctg cagcttcttt tgcagctaag cgccttgctt ctttcgcttc tgcgcgctgt
     4561 ttcattctct cttcctttgc cgcttgcttt cgagcttgct tggcaggatc accttccaat
     4621 aattcctgta ctcgttctcc aattgattgg ataccattta acagctgata gccttccaga
     4681 ttactgaaca ggaatgctcc tcctgccagt aatagcactg ctgctaaata acttccaggc
     4741 tgtgcaatca agaagtaagt aaaatgataa aggattgctc caatcatccc accaccaacg
     4801 ttattgtcta cttgactttg ttttatatca ctggctaaca aatcccatgt cgtccccatg
     4861 atatttggtt ccccagtatg gagtttgcca aataaatggg catgtaacaa taataagata
     4921 ccgaaataaa agagtatgcc gccaaaccag cgtcggtttt taccaattga aaactctgta
     4981 ttcttaatga caatccaaaa tcctaatacg gctaataaaa gacagaggat ttggtaagta
     5041 tttcctgcaa tgatccgaaa gccgtttgca atcaatattc ctaaaaaacc taattttaaa
     5101 aagccgaaga cagcaaataa aatcaaggca aacccaacac caatcgtcag catttgttcc
     5161 tgctgctgtt tttgcttttt cgtttttttc gtctttttcc tttttttagt aggccgtttc
     5221 ttttgtgcca tttattatcc ctcatttcat caagtttcat tatacaaaaa aaagtaaggg
     5281 aactaaagt
//
LOCUS       DAXWLH010000156         5234 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXWLH010000156 DAXWLH010000000
VERSION     DAXWLH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.160"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1340
                     /locus_tag="ACGE36_002320"
     CDS             <1..1340
                     /locus_tag="ACGE36_002320"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8384680.1"
                     /translation="ILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFN
                     GLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASE
                     AGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNV
                     KYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPY
                     SANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRG
                     AAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFV
                     KGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEE
                     PIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEMWLQGALEVFKHEQ"
     gene            1330..2529
                     /locus_tag="ACGE36_002321"
     CDS             1330..2529
                     /locus_tag="ACGE36_002321"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8384681.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            2542..>5234
                     /locus_tag="ACGE36_002322"
     CDS             2542..>5234
                     /locus_tag="ACGE36_002322"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFF8384682.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDS"
BASE COUNT         1843 a          891 c         1095 g         1405 t
ORIGIN      
        1 atattttggg atatgcaatt gcaccagagt atcttttcaa cgtcttagcg gatcaagcaa
       61 aacaagcaac gttccaattg aacgatctaa acaaagcctt tgtgcaattg gcatctacct
      121 acaatcaatt taatgggtta tttgatgatg tggatctcca atcaaaaaaa ctaggaacag
      181 atgagcaaca acgaaatgtg acgattaccg aagtcatcaa gaaattgaat gatgtagatg
      241 ttttagaaca cgatggggac gtgatcgggg atgcgtatga gtttctgatc agtcaatttg
      301 cttcagaagc agggaagaaa gccggagaat tttacacacc tcatatggtt tctgatatga
      361 tggcgcaaat cgtaacgctc gatcaaaagg aacgacgatt ctttagtgtt tttgatccaa
      421 cgatgggttc aggttcattg atgttaaacg tacgtaacta cttaacacac ccagataacg
      481 ttaaatatca tgggcaagaa ctaaatacaa cgacatacaa tctagcaaaa atgaacttga
      541 ttttacatgg tgtcgacgcc gaagaaatga atctccgcaa tggggatacg ttgaataagg
      601 actggccaac agatgaacct tacacatttg atgcagtagt aatgaaccca ccttattcgg
      661 ctaattggtc agcagatacg actttcttag atgattctcg gtttaatcgt tatgggaaat
      721 tagcgccaaa atccaaagca gattttgcgt ttcttcttca tgggttctat catttgaagg
      781 aaacaggtac catggccatc gtcttaccac acggagtttt attccgtggc gctgcagaag
      841 gggtcatccg tcaaaaatta ctagaagatg gcagtattta tgctgtgatc ggtatgcctg
      901 cgaacttgtt ctttgggaca tcgattccaa cgacggtgat tgtgttgaag aagaatcgtc
      961 aaactcgaga tgtattattc attgatgcta gtcgtgaatt tgttaaaggg aaaaatcaaa
     1021 ataaactatc ggaagaaaac attcaaaaaa tcctcgaaac ctacgctgaa agaaaagatg
     1081 tcgagaaata cgctcatttg gcaacctttg atgaaatcaa agaaaatgac tataacttaa
     1141 atatcccacg ctatgtagat actttcgaag aagaagaacc aattgatatg gttcatgtcg
     1201 gaaatgacat caagaagata cgacaagaac agcaagtgct agaaaaagaa cttttagaag
     1261 ctatctcttc cctgcaaaca acacctgaaa acgaaatgtg gttacaaggt gcgctggagg
     1321 tgttcaaaca tgagcaatga tacacgacca gaaatccgtt tcccaggatt tacggaagat
     1381 tgggaagagc gtaagttaat tgatttagtt gttagactta ataaaagtac aaactccagt
     1441 agacttccaa aagttgaatt tgaagatatc gtggcaggag aaggtagact gaataaagat
     1501 gtgagccaga aatttgataa tagaaaaggg attgaatttt taccaaatga tattctatat
     1561 ggaaagttac gtccatattt aaaaaattgg ctcaaggcaa cttttacagg cgtagcgatt
     1621 ggggatttct gggtttttag agctaagaag agcgactcgg actttattta ttctcttatt
     1681 caagctgata gatatcaaaa ggctgcaaat gatacatctg gaacaaaaat gcctcgttca
     1741 gactggaaaa aagtttcaga tacagtattc tatattccca atgatttaaa agaacaacaa
     1801 aaaatcggca tattcttcaa aaaactcgac gacactatcg ctcttcatca acgtaagtta
     1861 gatttgctga aggaaacgaa aaaaggattc ttacaaaaaa tgttccctga aaacggagca
     1921 aaagtgccag aaatccggtt tcctggtttt acggaagatt gggaagagcg taagttggga
     1981 gaattaggta aaactcaatc gggaattgga tttcctgatg ccgagcaagg tggttctgaa
     2041 ggtactccat tttttaaagt ttccgacatg aataacattg gtaatgagta tgaaatgaga
     2101 aatgcaaatc attatgtatc taatgagcaa attgagcgta aaaaatggaa gccaataaaa
     2161 gatgtgccag cgattatatt tgctaaagtt ggcgcagcaa taatgcttaa tagaaaacgt
     2221 ttggtcacaa gccctttctt aatagataac aataccatgg cttatttatt taataatact
     2281 tgggatatct attttggtaa aatattgttt gaaactataa atttgccaag atactcccaa
     2341 gtaggggcat tgccttctta taacagttct gatattgaga atatcagtgt taaggttcct
     2401 gtaaaggatg aacaacaaaa aatcggtact ttcttcaaac agctagatga tacgatcgct
     2461 cttcatcaac gtaagttaga tttactaaaa gaaacgaaaa aaagcttctt acaaaagatg
     2521 tttgtctagg agggaagaag aatgaaaatt agtcaccgtg aggaagcaga agtcgaagaa
     2581 cagttgattc gtgtacttgg agaagggcat aatcagtgga cgtatcgtcc ggatctaaaa
     2641 tcagaagaag acctttgggt caatttacgc caaaaaatta tctcaaataa tcaagcagaa
     2701 ctgaatgatt ttcctttgac cgataaagaa tttgaaacga tcaaaacgga attactgctg
     2761 cgaacgaaaa ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg
     2821 atcacgattg aacgggaaga ttcgcaattg ggttctgtct cacttatcct ttattccaat
     2881 caagacattg gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga
     2941 agtaacatag aagcacggga tcgacgtttt gacgtcacct tattaatcaa tggattaccg
     3001 attgtccaaa tcgagctgaa acaagtaact gcaaaagatg gtgtttatca ggcatttaac
     3061 caaatcaaaa aatatgcaga agaaggtatg tttcggaaca atatcttttc gaccttacag
     3121 ttgtttgtgg tttccaatga acaaaccact cggtattttg caaatgcctt accgaaagac
     3181 ttgcacccta agtttttatt tagttggcga acgaaagaca atgaaaaagt agaaaactta
     3241 tatgagtttt gtaaacaagt gttaaatatc ccggatgctc atcgtttgat tgcggattat
     3301 acaatcgtca gtgaagatca agataataaa accctcatgg tgttacatcc ttatcaagta
     3361 catgcgattc aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat
     3421 gcgactggtt caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa
     3481 tcggggatcg atcgaacgat catgttagtg gaccgcaaag atttagataa tcaaaccacc
     3541 acagagttta caaaatttgc ttcggaattt aatactggga tttcttccgg gaacgccaaa
     3601 gcgaatagct tgatcgttgg aacgggaagt gccaaagaat taagtgaaac cctgttggca
     3661 gatgccaatg caaatgttgt aatcattaca acccgtcaaa aactagatgc ggcgttgaaa
     3721 tatgcaaaga aacaagaaga gaaaaaagga accaatcgct tccaaaaatt aatgggccaa
     3781 catatcgtgt ttgtagtaga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa
     3841 atcaaaaaaa tgtttccgaa atccacttgg tttggcttta caggtacacc gatttttcct
     3901 gagaatcgga aacaagccaa agggcaattg gcacgtacaa cccatgatca gtatggagag
     3961 gtcttgcata cctataccat taagaatgcg ctagaagatg gctctgtctt aggatttcaa
     4021 gtagagcatg aaaacaccgt ggagccgact tcactagaga ataaaatcta tcggaaatta
     4081 aaagaagtag aaacatatgc agagtattca tcagaacaga tcaatcgtat gattgatcaa
     4141 atggaacctg tcaaaaaaga aagttatttg gacccagctg tttttgaagc agatgaacat
     4201 atccaaaaag tcattcataa aatgttccgt ccagataatg cctatacaaa atttgatttt
     4261 cgaaatggca ggccaacgaa atctgcaatc ttaaccacga gttccattga catggccaaa
     4321 aagtattatc gggccatcaa agagatgacg aaagagcctg attggttaac aaaagagttt
     4381 cccaatcagc tcattcgtga aggacgaacg atggaggatc ctgattttcc acgaatcgct
     4441 attacctatt cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa
     4501 aaaattattg aggaatataa tcagtattat gatacttcct ggtcattagc agacattgag
     4561 cgttataacg gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt
     4621 gggaaacaaa tcgatttagc gattgtagtg gatcgtctat taacaggttt tgacgcccct
     4681 acgattcaaa ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagctttt
     4741 tcacgtacga atcgtacgta tcctgataag acaaaaggat tgattgtgac gtttcgtaag
     4801 ccagcgacaa tggagaagaa tgtcgaggat gcgacaaaac tgtattcaaa agcaaaagaa
     4861 gaatcaggtt tgatttatcc aagttaccaa gaatccaaaa aaagattcaa acaagcatat
     4921 ggtaagttaa aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc
     4981 cgtatcgagt acgtcaaagc atttcaagaa ttgaatcgtg cttatgaatc gttagtgact
     5041 tatgacgatt acaatgatga catggtgaaa tctccaactt tgaacaatca aattcaactt
     5101 ttggaagaac aaattggcgt gtacgagacc gtgaagggtt cattaataga ggaagaacca
     5161 gaaaaagaag gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg
     5221 tatgatattg attc
//
LOCUS       DAXWLH010000157         5233 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXWLH010000157 DAXWLH010000000
VERSION     DAXWLH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.161"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..667
                     /locus_tag="ACGE36_002323"
     CDS             <1..667
                     /locus_tag="ACGE36_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384683.1"
                     /translation="VAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            711..980
                     /locus_tag="ACGE36_002324"
     CDS             711..980
                     /locus_tag="ACGE36_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384684.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1212..1508
                     /locus_tag="ACGE36_002325"
     CDS             1212..1508
                     /locus_tag="ACGE36_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384685.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1510..1932
                     /locus_tag="ACGE36_002326"
     CDS             1510..1932
                     /locus_tag="ACGE36_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384686.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1951..3543
                     /gene="mobP2"
                     /locus_tag="ACGE36_002327"
     CDS             1951..3543
                     /gene="mobP2"
                     /locus_tag="ACGE36_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8384687.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3564..3884
                     /locus_tag="ACGE36_002328"
                     /pseudo
     CDS             3564..3884
                     /locus_tag="ACGE36_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4045..5186)
                     /locus_tag="ACGE36_002329"
     CDS             complement(join(4045..4958,4958..5186))
                     /locus_tag="ACGE36_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8384688.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT         1854 a          827 c         1047 g         1505 t
ORIGIN      
        1 ggtggcctat tacttgttaa gtgagccaaa acagatcgcg gcgtcaaata ttttcacggc
       61 agtaatgaca ttaatgtgtg tcatgtgctt ttctattttt agttacttac tcgtccaatt
      121 tatcaggcaa aatttaacaa tgagtaaact tttcaaaagt ttattggact tacttatctt
      181 aggcagtatg ttaacgactc taacagatcg gttcgaacaa tctgaaaaaa cggtagaggg
      241 gataaagaac ttcttaacga cgattgatca atttataaat ggatatatgt atttgtttga
      301 agtaggcttt attctcactt tgtttggttg tgtatttata aaactcatta aaagatcaat
      361 cgaaaaagaa atggttattg atctaattcc gacgctgagc ggctatgagt attacgttga
      421 ttttggaaag tatgaggagt tgatccatac ggatatcgtt cacacgagta tccatgaagt
      481 aaccgttaag ggcatcagat ctactttaaa agatggacag gagacgaaaa caatcgacca
      541 aagaaaagag gtctacaatt tttataaagt tgaagcaagt ctacgatatg gcattgggaa
      601 attgaggtta gatgggagta atattcaaca aaacaaacca tatgttttag gaaaagtggt
      661 gatttagaac ttcctatgaa agatgaataa ataaggagaa tcttttgaaa atgattcttc
      721 tttacagaat tgtgcttata gttggcacgg agttaaaaat cttaaagaag gagcagttat
      781 tcaaagtgaa agaacatcat aatcagttac tatgtattaa aacgggtacg atgaacattc
      841 aataccgaac agaatattta gaattaacga atcaaactga ttttttgtcg attcgagagc
      901 aacataagca agaaaaatta atggctactt atagccaatt gattgatgag tgggaacaat
      961 tcaaggaatc taaaacataa aattccattt aaacacctta ttttttaaag agcataagga
     1021 aacgtgaccc cacttttaaa aaaagtggtt ggataggctg tgcctatcac gtttctgaaa
     1081 aaaataaatg accccaaaat accaatatat aggatagtac aaggatagca aaggatagta
     1141 aaaggataga ggtaggatag cgaaaaggat aggatccttt tcactatcct atattatatt
     1201 ggaggatatt tatgaatgtt aaaattagag atttagatcc aaaattcatt tctgaaattg
     1261 atcgaagatg cgttgaacta agtaatcgta caggaaataa atggagtcgt aatgattatt
     1321 taaagctact cgttgaaaat gattttgatc gtcctttgat ggaatacaaa caagaaaagt
     1381 ttgaccagtt attagaaaag ttttcagaaa ttcaaagtta taacacaaag atattagaag
     1441 aatatgttag ccaaaataat cgtattatag aaatcttgat tgagcaaaat agaggaagtg
     1501 aactttgata tggcaaaggt tatagacaaa cacatttact ttcaccaaac acatatcgat
     1561 gtattggatg aaactatttc tactcatgca gaggttaaaa actatagtga agctgttcgc
     1621 tttttatgta tgaactatga tccagaagtg gagaaaaaag taaattccaa tcaagcaaag
     1681 ttgaatggaa tgagtaaaga aatttctatt ttaaccgaaa tggtttcaga atttgcggat
     1741 cttcatttga aagatacagg aatattaatt ggtaaaaatt caactgttta tcaagaagca
     1801 aaagaacgag tggaaggaaa aattaaatcc aatcaaacaa aaaaatattc ccctcagttg
     1861 aagaaacctg tgaatgaatc tgttcatgcc agagtagatc catttgatcc aacgaagatt
     1921 ctaaaaagat aaaaatgaag gaggataata atgactcaat cgccttcagt tattctcact
     1981 tgtcaattta ctttgccgaa taaaaagtca ttttctactt atattgacta tattacaagg
     2041 gaacaggctc ttgaagaaaa ggaacgacgt acgcccgaag aagaacagga acttcaacga
     2101 atcaagaatg ctttagagga gttttatatt cctgaaggag aaactttttc ggagagtaag
     2161 acgggtaaag aattcagtga aacgtcgaat gaagctcaat cgttgatgga acatggaatc
     2221 aattttgaca agttagagga acaagatttt accaattatc tttcttatat gacacgtcac
     2281 tatgcgttag aacaaaagaa agaactgacg caaagtgaac aaaaagaata ccaacgcttg
     2341 aaccaagcaa tgaaaaaata tcaaacagaa ccccataaaa aagcttcagg aaatcttcct
     2401 ggagtttttt ctatagcttc agacgaagtg aaaggaagtg atctgaagga gattaagcaa
     2461 atcttccaaa gaggacaaca aaaaggctca atcatttatc aagatgttgt ttctcatgat
     2521 aattcgtatc ttgaaaaaat aggattgtac gatcccaaaa cagatacttt agatgaagaa
     2581 gggttaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga acaattgaat
     2641 gaaacaggtt actggatggc aacgattcac cgtaacacta aacatatcca tattcatttt
     2701 gcagttgttg aaaaagaaaa tacgcgtaaa ccttacacgg aggttgaaaa tggtgtgagt
     2761 tatatcgtac cgaaaggtaa gagaagacaa gcaacattag acgcaatgaa gaccaattat
     2821 gtacatgagt tagaacgttt ttcttatgaa aaggatcgaa tgattggtat ggagaaacga
     2881 aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt aaaagagcca
     2941 gcgcgttacg atcgacacgc gatgaactta ttaaatgaag tatataaaac cctacctgac
     3001 aaacgttcgg aatggaatta cggggatgaa aaacgaacca aactttcacc atacacaaga
     3061 gagaaactcg atgagttaac caaatatgtt ttaacaaaag aacctgaata tcaagaatat
     3121 atacagattt caaaagcatt gaaagaagaa gcaaaaagtc tttatgggga aagcaagaga
     3181 gaatcaaagg atgcagagaa aaatgctctc tttgatttaa aaaagcgcac aggaaatgcc
     3241 atcctaacag agctgaagag acaggatagt caaataaaac cattaataga aagtgttgat
     3301 cagatgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacaatt gccaacgaat
     3361 gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca acaaagagtt
     3421 gtcacgcagc ggagtttgcg tcaattaaat cgaacgatta atgcccatga agaaaaatac
     3481 cacgcaagta aagcatacga agagcaacaa cgaagaattc aaatggaaca agaaagacag
     3541 taaagacagt aggaggaata gcaatgaaac aatatttaga tcaatggaaa gttatagaag
     3601 gatcattaag agaagaacga atcgaacaac ttcctgattg tttagaaaaa gagcatttgt
     3661 tccagataag agaaatgttg agaaacgaac agtttgatcc aaatcaattt ttagtcgttg
     3721 agtacccagc tactggtgtg tattgttgta atcatgtgaa tggtgaaaaa tattttatca
     3781 ttcaagaata tgagggcaag ttagcacctt attatactac ttgggaaatg aatgaagagg
     3841 gaatcaataa ctttccatgt aaaagcattg gatttgagtc aatacctcgg acacgatttg
     3901 tgagaatttt ttcgagattc cagcgggtga gtatctcatc gtttcgacca gcggtcaaac
     3961 caaaaccgtt tgaccgctga tcgaagcgat gaagttgaaa ccctgtggaa atctcgccaa
     4021 aaaaatgtaa gtggtagcta ggaactaagc ttgcaatgcc aactcctcca tctgatttgg
     4081 tgttaaatag ttgatactac tatggattcg cttgcgattg taaaatcctt caatgtattg
     4141 aaataacgct aaataagcag cttcaaagtt ttcatacgct tttgatggat agacttcttc
     4201 ttttttgagt gaggcgtgaa acgattcgat accggcatta tcgtagggac agcccttacg
     4261 actgaaggag tgccgaatgt tcaattcatt cagctttgcc tcataggctt cgctagtgta
     4321 ctgagaaccc aaatctgagt ggatgatcaa gttctcggag ggtgtttggt ttaagagcgc
     4381 attgtcaagg gtttcgataa ccaattcttt cgtcatctgc ttgccaaact tccaaccgat
     4441 gactttcttt gtgtggaggt cttgaatagt ggagaggtag gtccagccat cttttagggt
     4501 atgaatatag gtaatatctg tcacccattt ttgattgatc gacgtagtgg taaaatcttg
     4561 gtttaacagg tttttacgtc cttcactttt aactttcgct tgtttgccag gcttgtactt
     4621 cttgagggtg actgaacgca agcctaattt tctcatcagt atttgtactt tctttaagga
     4681 caaggtctcg ccacggccaa caagaatctt atgaatttta ggcgcaccat agataccctt
     4741 gttttcaaag tagattcctg aaatcaagcg gtttaagcgt ttgttttctg attggcgctt
     4801 ggaaggtttc tttttcttcc agtcatagta gctggatttc ggtgcttcca aaacggtaca
     4861 catttgtttt acgtcatgat catgaacatt tgattgaata aattcgaaga catcactcat
     4921 ttttactttt gagcgaatat ggttaaagcc ttttttagga tagtgttttc ctcctgcatg
     4981 cgggccattt cctttttcat cgctaggatt tctgcctcag tcatgccggt tgctacattc
     5041 tttgtatgaa gcttcttcca acgataaatc gtttgtgtag cgataccata ttgcgctgcc
     5101 agttcttcga ccgattggcc agactggttt aaatctaaga tcatcttctt aaagtcttca
     5161 tcaaattttt tcttggcttt tgccatcgtg gacacgtcct tttcttagta gttagattct
     5221 accaaatcgt gtc
//
LOCUS       DAXWLH010000158         5225 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXWLH010000158 DAXWLH010000000
VERSION     DAXWLH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.162"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(61..276)
                     /locus_tag="ACGE36_002330"
     CDS             complement(61..276)
                     /locus_tag="ACGE36_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFF8384689.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            500..1300
                     /locus_tag="ACGE36_002331"
     CDS             500..1300
                     /locus_tag="ACGE36_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8384690.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1319..3187
                     /locus_tag="ACGE36_002332"
     CDS             1319..3187
                     /locus_tag="ACGE36_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8384691.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3180..3671
                     /locus_tag="ACGE36_002333"
     CDS             3180..3671
                     /locus_tag="ACGE36_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFF8384692.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3659..4213
                     /locus_tag="ACGE36_002334"
     CDS             3659..4213
                     /locus_tag="ACGE36_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFF8384693.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4231..>5225
                     /locus_tag="ACGE36_002335"
     CDS             4231..>5225
                     /locus_tag="ACGE36_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFF8384694.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1832 a          831 c         1069 g         1493 t
ORIGIN      
        1 aaccttccca ataaagggaa ggttcatgaa gataataact tttttaataa ttatagcttt
       61 ctaatatctt tcatcaactt ttctagtaat cattgggaaa aatttcaagc agtagatcgc
      121 acaaatacct acgacgatat aaatgatctt cgcaaaaata gttgttgaac caccagcaat
      181 catagctact aaatcaagtt cgaacaagcc taccaacagc cagttcaacc ctcccacaat
      241 caataaagtt aatgcaatag catctaaagt tttcattaga attctccttt cttaaatgat
      301 aaaacaatta tatatcaaaa agagaaacat tgcttatata acgctcagga attttagcaa
      361 tctatgcaaa aagatgaaaa ttttttttgc tcttccatta gtacacattt tttagctgaa
      421 gctgtaaatt tcatgtctta atttttttta gtgagctttt atactggatt tgtaagaaaa
      481 aagaaggaga ggatcacata tgtcggattg gttggatatt gcaggaaaag tagtaattgt
      541 aactggaggt tcctcaggaa ttggaagaag tatcgtcgaa aatttgttga agcagaatgc
      601 acaagtagcg aattttgatg taactgaatg ccgtatacag catgaaaacc tactatcttt
      661 aaaagtcgat gtgtcttcta aaacggacat agaagaaggg atatataaag taatgaaaca
      721 tttcaagacg atcgatggtc tagtcaataa tgcaggaata aatattcctt ctcttctaat
      781 agatagaaat catccaaaaa gcaagtatga attatcagaa caagtttttg ataagatgat
      841 cgctgtcaat caaaaaagtg tatatttgat gagccaagca gttggaagaa tcttggtgca
      901 aaagggatca ggagtgatcg tgaatctttc ttctgaatca ggattggaag gatcagaagg
      961 acagagctgt tatgctgcta caaaagcagc gatgaacagt tttactcgtt cttgggcaaa
     1021 agagttagga aagaggaatg ttcgtgtggt aggtgttgca ccagggatac tggaagaaac
     1081 cggtttgaga acacaagaat atgaagaagc tctttcttat acgagaggta taagcgtcga
     1141 acagctgaga aacggttaca gtcgaacaag tacgatccct ctaggaagga gtggtaagct
     1201 gcaagaagtg gcggatctag tctgctatta tctttcagaa agatcaagtt atatcactgg
     1261 agtcacgact aatatttcag gaggtaaaac aagaggataa aggatagggg ggacgcacgt
     1321 gacgctagta aacagatggt accaattatt gaaattacta gtcactcaca agaaagtttc
     1381 aatccaggaa gtgcaaaaag aaatcaaaac cagtaatcaa accataaaaa aagatattgg
     1441 ggagttgaat gaagaaattg ccggtattgc aaaaatcatc caaaaaaaca aacattttga
     1501 acttgaaatt tatgattttg atttttttga tgagatcatg caaggaaaat tgaaaacagc
     1561 cagtgatttc aattcggcat ccaaaagaat cgcttattta ctaaataggc tgatcgaaac
     1621 agaggatttc ttacgtatgg atgatttgtc tgaagaagtc ggtgtcagca gaggaacagt
     1681 ggtcaaggat ctaaagacat taaaagagat gatcaatgat tttgatgtaa agattacagg
     1741 cacaccaaat aaaggactaa gaataatagg agatgagcta gaattacgac tacttttatt
     1801 ttattttgtt tctccctact tttcagagac gaaaacagaa cagtttctag aagaaaaaca
     1861 agcaatcaaa cattttcaga agaagacgaa tgctagtaaa caagaaatca cccttttaac
     1921 gaaagttatc ggtatttctt tgaatagaat ctctagtaac tatctgttga agaagcccat
     1981 caatcgttac agtgggtgtt ttgaatatat ccaagaattc aatgaactga tcaatcagtt
     2041 ggaagaaatc tatgaactaa ctttgagcca atatgaaaaa gattttatca gttttccttt
     2101 gaacatcatc aacacaagta taacgatgat aaggaaagct aactctgatt tgcgcttata
     2161 tttcgacccc atgatgatgc gtattcgacg attagatctc attgatctgg atgagtcctt
     2221 cttatatcaa gagatgaaac agcacctctc tttattgata aatcgggtaa tttttcgtta
     2281 tcgattgact gatttatttt atggtgagat agaaaaaaag tatccttttt cttaccaaat
     2341 cgctagcgaa tgcatacaag cattagaagc tatcttgcaa cataagatct ctaaagtaga
     2401 gatcagctac ctaacgttgt atttggagtt agcatttcga tatcaaaaag agcatttttc
     2461 ctctaaaaaa gtagccattg ttcatacgac aggaaaagga acagcattaa tgatccaaag
     2521 acaaatcaaa aggatattgg ggagggatat acaaataaca catgtttcag aagaacaata
     2581 tgaaaaagta gatctaaaca aatatttcgc tgtttttaca acgattccgt taaaaaatgt
     2641 aaatgaagag acaccggtga tccatttgac taatcttttt gatgactctt ggttaagggc
     2701 acaatggcaa aaagcaaaaa aaaagcgatt agccgggttt cattctactg tttttttcta
     2761 tcaaatatgg aaagaaaaaa agacgtatca agaatattta aacaaattag tcgctccttt
     2821 gatcagaggc tattatgtgg atgaagaatt tgaaaaaagg atcttgaaaa gagaatatca
     2881 gcaaagcaca tttgttaaca aagaatttgc ttttcctcac acaaccaacc agctatctca
     2941 acacattgtt ttatcagtag ggctaataac tgatcaaggt ttagcagaaa caggaggaaa
     3001 tggagttatt tttttatttg ccatacctga agaaatgacg gctgaagttg agaatgaatt
     3061 atttgagcta tacgatatcc tatttgaact cataaaaatg ggaatagatg agtttattga
     3121 attagcaccg acacaagaat catttttaaa actaatcaaa agtaaaggag agagacttca
     3181 tgaatgatat gtatctattc atagttttcg cagcttcagc ttatgtgctg caagcagtta
     3241 tcagttggaa gcaactaaag catttcaatc aaacatatag cgaactgcga agaaaaggaa
     3301 aagtagcgat tggtaaacga cctgggaaaa tccgttcagg cacgattgtt atgctagcta
     3361 tagacgatca tgatcaagtt ttagaagtca gaaaaatgca aggggttaca tctttggcaa
     3421 aatttcattc attacctgtc tatgcgggtg aagatatcca ttacatcgat cgttaccatc
     3481 cctcagttcg agcggaagat cgtttgacac aagcagcttt agaaaatgcc agagaagtat
     3541 acttacgaat agaggcagga aatgaaaaaa gtgaacaacc ttcattgcct tttattgcta
     3601 tgctcaataa ttttcggata tggaaaagta cgatcaaaga aaaaaggagt gacaaacgat
     3661 ggatatcata acaaaatttg ctgagggatt tatgaggctg ttccaaacag gtgcagaaac
     3721 attcataagc tggatgacag gaatcgtacc agttgtcttg atgctgatgg ttgcaatgaa
     3781 tacgctgatt gcttttttag gtgaagaccg agtaacgaaa gtagccaaat tatcagcgaa
     3841 aaatccgctt actcgttacc ttgtgttacc gttcttatct gcttttatgt tgggaaatcc
     3901 tatgtcattt acaatggctc gtttccttcc agaatactac aaaccaagct attatgctgc
     3961 tcaagctcaa ttttgtcata cgagcaatgg tgtattcccc catattaatc ctggagaatt
     4021 gtttgtatgg atggggattg ctcaagggat cgaaacctta gggctaaatt cgatggagct
     4081 ggctatccga tacttgcttg tgggactgct gatgaacttt attggtggtt ggataactga
     4141 ttttactact ggatttgtat gcagacagca aggaatcgtt cttagcaaaa aagtagagct
     4201 ttcagttgat tagagaaagg agagttttaa atgtatggtg atattaagat cgtaagagga
     4261 aacggcgggt atggaggtcc tttgattatt aggccgacaa aagataaaca caagttcatc
     4321 tatgtcacag gtggaggtga gaggccaatg atcgtagata aaatagaaga actgacaggt
     4381 atggagtcta tcaatgggtt caaaacatca atacctgata gtgaaatcgc gcttgcaatc
     4441 gttgactgtg gagggactct tagatgtgga atttatccta aaaaagggat accaaccatc
     4501 aatatcgttt ccacaggaaa aagtggccca ttagcacaat atatcactga agatatttat
     4561 gtttcagcag tagggttaga acaaatacag ccactaatgg aaactgatga taacaaagaa
     4621 acagtaaata ctgcatctcc agataaaaag tacaaatatg atacttctaa aaagataacc
     4681 gagcaaaaag cagagcagca aaactggata gcaaaaattg gaatgggcgc gggaaaagta
     4741 gttgctacat tcaatcaatc agcaagagaa gcagtagata cgatgatccg gacagtgatc
     4801 ccttttatgg ctttcgtttc ccttttgatt ggtgtgatac aaggttctgg gatcggtaat
     4861 gtatttgcta acattatggc acctttagca ggaaatgcag ttggattaac aatcattggc
     4921 tttatttgtt cactgccatt tttatctcct ttgcttggtc caggggcagt tattagccaa
     4981 gtgattggaa cattgatcgg tgtagagatt gggaaaggaa atattcctcc ccaattagca
     5041 ttaccggcac tatttgctat caatactcaa aatgcatgtg attttattcc agtcggtcta
     5101 ggtttggaag aagcaaaagc tgagacagta gaagtaggtg taccatcagt tctttattca
     5161 agatttctta acggcatccc acgagttatc attgcttggg tagcaagttt tggattatat
     5221 agctg
//
LOCUS       DAXWLH010000159         5136 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXWLH010000159 DAXWLH010000000
VERSION     DAXWLH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.163"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(105..563)
                     /locus_tag="ACGE36_002336"
     CDS             complement(105..563)
                     /locus_tag="ACGE36_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8384695.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(576..1907)
                     /locus_tag="ACGE36_002337"
     CDS             complement(576..1907)
                     /locus_tag="ACGE36_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFF8384696.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1918..3360)
                     /locus_tag="ACGE36_002338"
     CDS             complement(1918..3360)
                     /locus_tag="ACGE36_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8384697.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3347..4111)
                     /locus_tag="ACGE36_002339"
     CDS             complement(3347..4111)
                     /locus_tag="ACGE36_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFF8384698.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4215..5090)
                     /locus_tag="ACGE36_002340"
     CDS             complement(4215..5090)
                     /locus_tag="ACGE36_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8384699.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1559 a         1086 c          747 g         1744 t
ORIGIN      
        1 caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa
       61 attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa
      121 ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga
      181 gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata
      241 gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt
      301 ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc agattcactt
      361 tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca
      421 tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag
      481 acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg
      541 tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt
      601 ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt
      661 tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact
      721 agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt
      781 tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact
      841 gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga
      901 cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa
      961 ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta
     1021 tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca
     1081 acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa
     1141 tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta
     1201 aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct
     1261 cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac
     1321 gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa
     1381 atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca
     1441 gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt
     1501 atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg
     1561 agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct
     1621 cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca
     1681 agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata
     1741 aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt
     1801 tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac
     1861 gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta
     1921 ttcttctaat aatttattga attctgccat aacataagtc acttctaagc cgatgataat
     1981 ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg
     2041 ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac
     2101 atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata
     2161 atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc
     2221 cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac
     2281 gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat
     2341 tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt
     2401 gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat
     2461 aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt
     2521 tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt
     2581 atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa
     2641 aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc
     2701 cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg
     2761 cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc
     2821 catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa
     2881 gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc
     2941 gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca
     3001 tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat
     3061 aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc
     3121 tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt
     3181 tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga
     3241 taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc
     3301 aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa atatcctcat
     3361 atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta
     3421 aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat
     3481 ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta
     3541 aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc
     3601 ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa
     3661 agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt
     3721 catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat
     3781 aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg
     3841 gatgctgata ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt
     3901 tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt
     3961 cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg
     4021 tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa
     4081 aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat
     4141 aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata
     4201 gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt
     4261 tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa
     4321 tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct
     4381 tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc
     4441 agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc
     4501 gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg
     4561 atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg
     4621 attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct
     4681 ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata
     4741 tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc
     4801 agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca
     4861 ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca
     4921 tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct
     4981 attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt
     5041 aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt
     5101 gtctccaaac aatttttaaa gacaaaaaac gaccta
//
LOCUS       DAXWLH010000160         5123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXWLH010000160 DAXWLH010000000
VERSION     DAXWLH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.164"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            99..827
                     /locus_tag="ACGE36_002341"
                     /pseudo
     CDS             99..827
                     /locus_tag="ACGE36_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(878..2050)
                     /locus_tag="ACGE36_002342"
     CDS             complement(878..2050)
                     /locus_tag="ACGE36_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFF8384700.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            2151..2657
                     /locus_tag="ACGE36_002343"
                     /pseudo
     CDS             2151..2657
                     /locus_tag="ACGE36_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2815..3432)
                     /locus_tag="ACGE36_002344"
     CDS             complement(2815..3432)
                     /locus_tag="ACGE36_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8384701.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            3696..4076
                     /locus_tag="ACGE36_002345"
                     /pseudo
     CDS             3696..4076
                     /locus_tag="ACGE36_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4076..4873
                     /locus_tag="ACGE36_002346"
                     /pseudo
     CDS             4076..4873
                     /locus_tag="ACGE36_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            4950..>5123
                     /locus_tag="ACGE36_002347"
     CDS             4950..>5123
                     /locus_tag="ACGE36_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8384702.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKD"
BASE COUNT         1729 a          911 c          895 g         1588 t
ORIGIN      
        1 tatggatacg ttcaaaaaga tttgaatttt acttttgact gcatatacta aaagtagcga
       61 aatatactgc cttacttttt tattaaggag tgaggaagtt gcctacgaag ctttcaaatg
      121 gaaaatataa aactaatttg agatatccaa aaaaatttaa agaaattaca ggtatcagtt
      181 cagaaaaatt ccaaaaaaca tttccaacta ggcaattggc tattaaagcc gaaaatgaaa
      241 tgaaaaagaa aatcgcaaaa gtcttaagag aggagaatgc aaattcatta gagctcaaag
      301 gtcaaataaa attcaaggag ttttataaat ctaaatggct tcctagatat gagttagggc
      361 aaactactag atcccataaa gctccaagcg atataaccat cattaataca aagaatttat
      421 ttcgattgca tattctgcct atatttggtg agtatgccat gagctatctt aatgcaaata
      481 ccgaaataat atcagatgaa ctcacaaaaa agtcgaagga atatgcaaac ataaaaatca
      541 ttaaatgcta tgttcgttcc atgtttgata ttgcagaggt tctaaattat attgaattta
      601 atcgtacaac aaaaataatt caaagtatta ctgcacctaa gaaagttgct ctggaagaaa
      661 aaagaatacg agaaggaaaa caagctttat cttcgaaaga actcactgat tggttagacg
      721 ctgtaagaga tgatttcaat gatcaattac tttcctttca cgattacact ctttttatgc
      781 ttactttata tcttggggat agaaagagtg agacctatgc tttgcaagtc aagtccataa
      841 tcctgtgtaa aatactatac aatgttttac cggtctctca ttgatcaaac ttaatatatt
      901 ttcttgtaga actgagccat tcgtcatgaa ggtccataag gacagctcca attaatcgat
      961 tggcagacgt tcggttggga aaaatccgaa taatcttttc tcttctgcgg acttcctggt
     1021 tcagtcgttc aagaaggttg gtgcttttta gccgattatg actatttccg ataaccgtgt
     1081 attgaaaggc atcttcgaag ccattatcca atatttcgca ggcttttgta tatttaggct
     1141 ggtcgatata ttcaccgact aaggcattct tagctgttcg agcgagttca atatccgtaa
     1201 acttaaagat cgcttttact gcttctctaa acggttttga attctttttt ggaatggaac
     1261 tgaagatgtt tcttaaaaaa tggacctggc atctctgcca acttgcgttg gtaaatgact
     1321 tacgaatcgc agacactagg cctttatggg catcagaaat gatgagttct gtccccttta
     1381 ggccgcgttc ttttaagtat tcaaagaaga tggaccatgt gtcatcactt tcttcatttt
     1441 gaatcatgaa gccaatgatt tcacggtcgc caccttctgt tatcccgatc gcaatatggc
     1501 agcttttaga aagcactcga tggtcctcac ggacttttat gtagagaaca tcagtcatca
     1561 gataaggata attcgtacct gagagtgaac ggttctgcca ttcgttgacc atcgggtcta
     1621 actgctcagt caggctagaa acaaaagatt tcgatacaga ttttccacat agctcttcaa
     1681 caatctttga aactttacgt gtcgaaacgc ccgaaacata catctcaagc attgaagcga
     1741 gcagtgcctt ttcatttcgc tgataacgct caaacaccgt cggtgaaaat tcaccatcac
     1801 gtgttctagg cacttttaat tcgagtgtac ccacacgagt cgtaaagtct cgctcataat
     1861 agccatttct ttgactctga cgactttctg aacgttcata gtcatcggct tgaatatatt
     1921 ctgttcgttg attttccatc aattggttga aaacagtggt taaaatattt ttagaaacat
     1981 catcttttac cgaatgttca ataatacttt gaacctcttc gttgttcagt gtaaaatgta
     2041 cttgggtcat gtaacgtcct cctgggtatg tttttgtggt taaaaacatt gtaccgtaaa
     2101 agggctgtta catggccttt tatcttttac acaattatat ggactttatc gctttgcaat
     2161 ggaaatacat tgattttgaa aaccaaacag tccgtcttaa atatgccttg gataaacaca
     2221 caaaaaagaa atttacaaaa ggtcgaaaag atactattat ccaagttccg gaaattgtaa
     2281 tgacactatt gcaagaatgg aaatcagtgc aagctgaaca actattgaag ttgaaaatta
     2341 aacagacacc tgaccaatat ctttttagtt actccaaacc ttctggtgaa gtaaattgtc
     2401 cagtccatac tgattactta aattatcgaa ttaattctat aaaaaaaaga catccccatt
     2461 tggttcattt aactcctcat aaacttcgac atacttacgc aactttagct agacaaggtg
     2521 gggctgatat gaatcaaatt tctaacgctt tgacacattc aggtatttca actacaaaaa
     2581 tttatgtgaa tacacctgat ttagttgata aagctgtgtt cgaagcgttt cagcgaggat
     2641 tacaaaaatc taattaatgt gttctagaat gtgttctaaa tataaaaaaa agacactttc
     2701 caaataattg gaaagcgtct tgaacgttga tatataaagg atattaacgt tttgagaatt
     2761 gagatgcttt acgagctttc ttaagacctg gtttcttgca aaattatatt agcctcaact
     2821 gaaagattta tttttttgct ccctaacttc attagtaata ccatgtttca ttcgatatct
     2881 tctaaacgtt ctacgattaa tccctgttag ctcttcaact tctttatctg tagatccagt
     2941 caaaaacaaa ttaaatgcta attgaagtcg cggatcattc tcacgatatt ttagcgtacc
     3001 tcccttgtat ttcccttttg ctttagcgag tgcaattcct tgttgttgcg tttcgcgaat
     3061 ataccgacgt tcttcttcgg cctggtactt gtatagttcg ataattaagt tattaagtaa
     3121 tcgacgtaaa ttatcatcag tgatacctgt taacgctggc aaattcaaaa cttccagtgt
     3181 agctttcttc agctgaatta aattcatcgt ttctgttagc tctttattat ttcgtccaag
     3241 tcgttttaat tctgtcacaa cgacaatatc accttcacgt atgaatttca tcatttcttg
     3301 aaatccttct cgattggtat ctttcccact tgctttatca ctaaaaattt tatcacactc
     3361 tgataataat tctaattgtc ggtctaaatt ttgatctaag ctgcttactc gagcataccc
     3421 aattttcgcc ataataaaac tcctttaaaa ttctgaaatc cggatcattt ttaaattctg
     3481 ttgcccaact ctgttgtcat ttttctagat aaatggcata aattttcagc gctaagtgac
     3541 aaaagcaact aatttttcat gatagatttt taaatatctc aatctatctc caaaattagt
     3601 tgcttttgta gctatagttg actatgaata tgacaaataa ttagaaaaaa ctgtgccaat
     3661 taaatatcaa aatgacaaat tgatgaatca aattcaaatt aaggggggca accctggaag
     3721 tcttaaattt accctcaatg aatggtattg aagatgaaaa tttaaggcgt ttgattaata
     3781 gccttgtcat tgaattgtac aagtatcaag cagaatcaga acgaaaaaaa attaaggaac
     3841 gtcaggcaca aggaatcgaa attgctaaga aaaaaggcaa attcaaaggt cggcaactga
     3901 aatttaaaaa aaatgatcca cgtttaaagc atgctttcga tttgtttttg aatggtttat
     3961 ccgataaaga agttgaagaa caaactggga tcaatcgccg aacgtttaga aggtatcgag
     4021 caagatataa cgtgacagta gatcaaagaa aaaacaatga aaagagggat agttaatgag
     4081 tacggttatt ttagctgaaa aacaagccag gcattagcct acgcaagtgc tttaaaacaa
     4141 agcaccaaaa aagacggtta ttttgagatc aaagacccac tatttacaga tgaaacgttt
     4201 atcacctttg attttgggca tttagtggaa ttagcagaac caggtcatta tgacgaaaag
     4261 tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga ttttgaagtt
     4321 gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa ggcaaataca
     4381 attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc gattatccat
     4441 aaagcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa tagcttagaa
     4501 aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta ctatcccttt
     4561 tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa cgcaagccct
     4621 ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact aggacgtgtt
     4681 caaacgccca ccttatacct tatttttcag cgccaggaag ctagtataaa agtaaaacaa
     4741 gggtcattta agggcgttct aagccccaca cagcgtttta aaacccaaga ggagcttttt
     4801 tgcttttgtt tcttctaaac aagctaaaat aggcaatcaa gaggggataa ttgctgatgt
     4861 tcaaaccaaa gagatggata ggctacaatt aactagacag aaaaattaag gtgtgtagac
     4921 tagaagaaaa tataccagga ggaattttta tgtctaagag aacacgaaga actttttcac
     4981 aagaattcaa gcaacaaatc gtcaatcttt acttagctgg aaagccacgt gtagaaatca
     5041 ttcgagaata tgaactaacg gcttcagcat ttgacaaatg ggtaaagcaa tctaaaacga
     5101 gtggttcatt caaagaaaaa gat
//
LOCUS       DAXWLH010000161         5101 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXWLH010000161 DAXWLH010000000
VERSION     DAXWLH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.165"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(27..1175)
                     /gene="nagA"
                     /locus_tag="ACGE36_002348"
     CDS             complement(27..1175)
                     /gene="nagA"
                     /locus_tag="ACGE36_002348"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFF8384703.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1251..2087)
                     /locus_tag="ACGE36_002349"
     CDS             complement(1251..2087)
                     /locus_tag="ACGE36_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384704.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2271..3488)
                     /locus_tag="ACGE36_002350"
     CDS             complement(2271..3488)
                     /locus_tag="ACGE36_002350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8384705.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3637..3798)
                     /locus_tag="ACGE36_002351"
     CDS             complement(3637..3798)
                     /locus_tag="ACGE36_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384706.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3871..3954
                     /locus_tag="ACGE36_002352"
     tRNA            3871..3954
                     /locus_tag="ACGE36_002352"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3905..3907,aa:Leu,seq:gag)
     gene            complement(4194..>5101)
                     /locus_tag="ACGE36_002353"
     CDS             complement(4194..>5101)
                     /locus_tag="ACGE36_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384707.1"
                     /translation="RGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVN
                     GRYNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSF
                     GKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTY
                     VNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNK
                     KTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSV
                     QKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1569 a         1044 c          810 g         1678 t
ORIGIN      
        1 tagttttaag gttaacttaa attatattat gcgtggaaac gttccacgcc atctagataa
       61 gttgcttcca atgtcatatc agggttcaat acgataaagt ctgcatcgcg tccttgagcg
      121 atttttccac atgtatcatc gattttacag ctgattgctg gaatcagact agccatcatg
      181 actgcttgtt ctggtgttgc taatccccag tctacaacat ttttaattgc ttctttcaat
      241 ttcaatatac ttccagctaa gttgccagat tctagacgtg ctgttccttc ttttactact
      301 actggaaatt cgcctaaatt gtaatttcca tcaggcatcc caccggccat catacagtct
      361 gtaattaatg cgacatgatc atgtcctgct ttttccatta ggattcccgc agcatttgga
      421 tgtacatgat ggccatcaca aatcaattca gaaaagacat gttctaaaga taataacgca
      481 cccaccatac ctggttcgcg atgattcaat ccacgcatac cattgaaagc atggacaaat
      541 acacttgcgc ctgcttcaac agctttttgc gcttcttcta gtgtagcatt actgtgtcct
      601 agagaaacaa ctactccttc ttcagtcact tgtttgacaa actcttctac accttgacgt
      661 tccggtgcta aagcaatttt tttgattaaa ccacctgaag cttcttgcca ttcatggaaa
      721 gtatccaaat caggatcccc gaaataactt gggttttgtg cccctttgtg ttcttctgtg
      781 aagaaaggac cttcaaaata gattcctttg attttagctc ctggtgcttc ctgatacatt
      841 ttgccgattg tctcagctac atcttttaaa cgttctttgc ttgacgtcaa tgttgttggt
      901 aaaaatgaag tcactccaca agataacaga ccttcagaca tcactttgat gccttctgca
      961 tcattatcca ttacatcatg attcatatag ccgtgaatat gtgtatctac caaaccagga
     1021 gcaatccatt tgccgctttg gtcaatgatg ttgacttctc cctcaggtag ttctttggtg
     1081 tattcaccaa aaagtccatc ggtaatttca agatagcctg catgtttgac acctgacgct
     1141 aaaaagaatt tgtcagcata gataaaagtt ctcattctat tctctccctt tgtttttcta
     1201 cctctaaagt actcttattt agagaagaag tcaagaatgg tctacaccat ttacaaattt
     1261 tttgaaattt gtttcacagc ttgtgctaag ccttcataga atgctgctct ttctggttct
     1321 tctttacgct tgtatattaa gcttttaagc acaaataaat cattgaggta atagtagcta
     1381 ttcttctctt gagcccaata gattccttga ttcacctgtt ctaacgcttc gtcgatttca
     1441 ttttgatgga ataagaaatt tccataatta taaaaaattt gcgaaaattc actttttgat
     1501 cgttcatcag ctgattgatg cagcagttgg atgcttcgac tcaaatagta gcgtgcttca
     1561 gccgtttttc ctgccacacc atatattttt ccgatacaac taatcagttg tatttctgtg
     1621 tcagaaacat ggattctttc tttttggcta gagtagctta atgcttcttt caaataataa
     1681 agggactgag agatattttg acgcaacgtg aattcgcaaa caccgagata ataataatag
     1741 cgttgaaagt cagttgcttg atacagattt tcagaaatat cagaagattt tagtacttgt
     1801 tctaacttcc gataatcacg tttttggaaa caactatcca acagttcaaa taatttatcg
     1861 tttgtccgag tagtttttcg agagagattc atcacatgat caatcgttac ccctagccgt
     1921 tgacaaatct gcatcatcac caatacattc ggcagttcct cattattttc gatccgactt
     1981 aatacacttt gtgaacaaat atcatgcgaa agcatttttt gcgtcatttt ttgttctttt
     2041 cgaatctctt taatgatcgt tccaaaggac tcgattaacg tttccataag tatcctcccc
     2101 aaaatatgcg tattcgcatt ctattcgtat tttactttag gaagtcgcaa acttcaagaa
     2161 aagacttgca actaaataat atactttagt tatttaagaa aactccacta gaaagagagc
     2221 ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg gtttttaaaa tcatttttgg
     2281 cgataggttg ctaggaattc tctcattttc ttagctgttt gtttcaaatc atccatttta
     2341 ggaagataaa cgattcgaaa atgatctggc tggttccaat taaatccacc accatgtaca
     2401 agtagaatat gatgttcatg taaaaagtcc aaaacgaatt tttcatcatc caaaatattg
     2461 aatcgctttg tatcaatttt tgggaatata taaaaagcag cttttggttt tacggctgaa
     2521 agaccaggaa tatcattgat tgcattatag atatattctc tttgttcata gatccttccg
     2581 cctggcagga gcagctcatc gacactttga taaccgccta gcgcggtttg gataatttgc
     2641 tgagacaata cattggaaca caaacgcatg gaggacaaca tattcagccc ttctatatag
     2701 cctttgacgt gtgacttatc tccacttagg accatccagc ctactcgaaa tcctgctaca
     2761 cgatgagatt tggatagacc gttcagcgta acaacgaaca aatcaggcgc taatgtagca
     2821 atcggaatat gttctagacc atccatcact aagcgatcat agatttcgtc ggaataaata
     2881 atcaggttat tttgtcgagc tacttcaacg atttcttcta ataattcttt tggataaagt
     2941 gccccagtag gattattagg attaatgata acaatagctt ttgtccgact cgttattttt
     3001 gatttgatat catcaatatc aggataccat tctgcctctt catcgcaaat ataatgaaca
     3061 ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg gatagtccgg cattggaacc
     3121 agtacttcat ctccattgtc cagcaacccc tgcatacaca tcgtgatcaa ttcgctcact
     3181 ccgttgccgg tgtagatatc attgatcgtc acattcggaa aatgtttcag ttggcaatat
     3241 tgctcaattg cttttctagc agaaaagatc cccttggaat ctgagtagcc ttctgagtca
     3301 cgggcattca tgatcaaatc acgaatgact tcgtttggtg cttcaaatcc aaaaggtgcg
     3361 ggatttcccg tgttcaattt taaaatacta atgccctctt cttgcatccg ctccgcttct
     3421 tctagaacgg gaccgcgaac atcgtaactg acaccttcta atttgttgga tttttcaaat
     3481 tctctcatca cttagccaat ccctcatttc aaaaagttag aactttccaa attttttgtt
     3541 atttactatt tcaatatcat actccatcct agaaaatttt gccacttatt taggaaaaac
     3601 gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa ttgtcatgat agtcattcca
     3661 tagattcgct gcccaatcca ttgcaattcc acaaatgatt cctagcataa aaccaagcaa
     3721 aaaacgtccg aatgcaagtc caatcaatac acctaagatc attcccagtc ctgaaaattc
     3781 tccgtcatat ttctccatct taataagtcc tcccattttt gagcttgaca actatgtaaa
     3841 acctcgctat aattagtgtg ttattattac gcagatgtgt tggaattggc agacaagcat
     3901 gattgagggt catgtgagcg ttagctcgtg tgggttcgag tcccatcatc tgcactacaa
     3961 ctcgtgaagt taattttgct tcacgagttt ttttgtttga acgtttcttt atgccattat
     4021 atacatatca ctgtatcctt tacaaaaaga acgtacaatc atgtaaacag cctgaggtga
     4081 gtatatgaag gtcggaaaca agctactcgt agaaacttgc aagtgttaaa acaattaatc
     4141 gaaaaacgag taaaatccct cacctaatac taaagcgagg gattttttgt atattatctg
     4201 tttatcaccg ttattataaa ggtacaaata ccaagcacgg ttaatccaaa ttgcataaat
     4261 tcattttttt gtacactcat gcttttcatt agctctttaa ctgccattag tactaagatg
     4321 gcgataacaa cataaagtat atagtacgtc cgcattccgg aagcccatac tgtgggagaa
     4381 aatcccataa ttaaccttga acaaaatccc attatcatca aaataactaa aaaaatagaa
     4441 gttttcttat tgtcaaaact taaataaatt ccaacaatta aacatagtaa taatgccaag
     4501 actactaaag tagctatcca agtacctgga taaaaaagtg acagtttcgt tcctaactct
     4561 gtaaagagat tattcaggtt actactactc caaatcattg cccttttatt tccattgacg
     4621 taagtaaagc tttgacccat tgtattacca aaataaataa tcaggtttaa gaaaaacggt
     4681 aatgcagtca atatacgcac ataatagttt tgacatttcc tatatgttag aaggaaaatt
     4741 aagaaaaata gcaataaaaa taagatattc atgtctaaaa aaaacggctt tccaaatgat
     4801 gaaataccta aatctaactt attaagaaag ttgaaatttt tatattcagg aaaccattta
     4861 ttaatttcat gtgcagcacg taatgcattc cctggagttg tcaaagagaa aattaattct
     4921 gctaagctta ttattgaaaa tactgataac ttataattat atcgtccatt gacaatcaaa
     4981 tataaactaa ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc ttgattagtt
     5041 aaaaatatca ataaaggaat acttacccaa tatatttttc tatttatttc ttctcctctt
     5101 a
//
LOCUS       DAXWLH010000162         5056 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXWLH010000162 DAXWLH010000000
VERSION     DAXWLH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.166"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(128..613)
                     /locus_tag="ACGE36_002354"
     CDS             complement(128..613)
                     /locus_tag="ACGE36_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFF8384708.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    703..1048
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1093..1734
                     /locus_tag="ACGE36_002355"
     CDS             1093..1734
                     /locus_tag="ACGE36_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8384709.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1753..2388
                     /locus_tag="ACGE36_002356"
     CDS             1753..2388
                     /locus_tag="ACGE36_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8384710.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2406..3221
                     /locus_tag="ACGE36_002357"
     CDS             2406..3221
                     /locus_tag="ACGE36_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8384711.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3540..4976
                     /locus_tag="ACGE36_002358"
     CDS             3540..4976
                     /locus_tag="ACGE36_002358"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8384712.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         1664 a          840 c         1105 g         1447 t
ORIGIN      
        1 tcgtattagt tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt
       61 ctgatatatt ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac
      121 tctatcatta caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa
      181 agtcggaaaa atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat
      241 cttgacgatc aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca
      301 atgctaatgg agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag
      361 aaacaaaaga taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct
      421 tttcatcagg caagatgaca agatagattt gttttccttt tcttgatttc aataatagat
      481 tttttacttg cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat
      541 gatcgacttt ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg
      601 ttgcacgact catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg
      661 acaaatgaaa aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga
      721 agagtagccg ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac
      781 cgtgaaacac atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc
      841 gttacagctg cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg
      901 taaaaaaagg tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg
      961 ttttcatctg aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag
     1021 gtggaaccac gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag
     1081 gaggaaaaga agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc
     1141 acaacattga aattattttt atttaccttg attggctcat taccattagg gattgtttta
     1201 gcattaggat tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg
     1261 gttatgcggg gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg
     1321 attgggatcg tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca
     1381 gcttattttg cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa
     1441 gcagcgcaag ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa
     1501 gtgacaaaga ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct
     1561 tccttgatct atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa
     1621 cgcgacgcga ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt
     1681 attctaacaa tcgtaatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata
     1741 ggaggaagaa atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata
     1801 atcaaagatt tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc
     1861 ggtggcggta aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga
     1921 gaattttatt tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt
     1981 gtaggagtcg tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt
     2041 accttagcac caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa
     2101 gaattagcgg cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct
     2161 ggcggacaaa aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg
     2221 gcttatgatg aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg
     2281 atcctatcat tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt
     2341 gcagaaaatg tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga
     2401 tagagatgaa aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag
     2461 gctgttcgaa caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa
     2521 tcgttatagg tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc
     2581 tcacaggatt tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag
     2641 actttcagcc cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt
     2701 tgatatggaa cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac
     2761 cttatatgga aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg
     2821 gacaaatgaa taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta
     2881 cgaaacaacc agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata
     2941 gttttaatga agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata
     3001 aagtatacgc agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa
     3061 aaggttctta tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag
     3121 tgaaaaaaat caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat
     3181 ccgataaatg gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca
     3241 aaagacgtgt cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga
     3301 ctcggagctg aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt
     3361 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac
     3421 tactcgaaac taagcgactt tttcacaacc tcctttacat atttttattt tgttgtggaa
     3481 ggggtttcgt gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa
     3541 tggaacacaa agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag
     3601 cgtatcaagt agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat
     3661 ttgtacggat acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact
     3721 atcatcgtta taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct
     3781 tctctgtagc ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc
     3841 tcgcttttta tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga
     3901 ctttatatca ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac
     3961 gtcagatcat tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga
     4021 aagtccatta ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt
     4081 tagcagcaca tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg
     4141 ctaatttagc aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa
     4201 tcggaccaag ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc
     4261 tggctgctga aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg
     4321 gaaaatatcc gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg
     4381 aagctggcga tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact
     4441 atcaaacggc gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata
     4501 cgacaggaaa aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg
     4561 agaatccatt tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga
     4621 tcggactgcg tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct
     4681 taggagagta tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt
     4741 acttacaaag ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag
     4801 gctattgcac ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat
     4861 atggatttgt atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga
     4921 aagacagtta ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca
     4981 catacttaaa acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt
     5041 tagaaattaa gaaaaa
//
LOCUS       DAXWLH010000163         5052 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXWLH010000163 DAXWLH010000000
VERSION     DAXWLH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.167"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACGE36_002359"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACGE36_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8384713.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACGE36_002360"
     CDS             complement(2066..3064)
                     /locus_tag="ACGE36_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFF8384714.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACGE36_002361"
     CDS             complement(3290..3943)
                     /locus_tag="ACGE36_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFF8384715.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACGE36_002362"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACGE36_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFF8384716.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1498 a         1038 c          767 g         1749 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttttat
     4981 gttttttatt tctttaatag taatgttttc tgtcatttta aataatactg ttctttatta
     5041 taacgcttat tt
//
LOCUS       DAXWLH010000164         5052 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXWLH010000164 DAXWLH010000000
VERSION     DAXWLH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.168"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(213..683)
                     /locus_tag="ACGE36_002363"
     CDS             complement(213..683)
                     /locus_tag="ACGE36_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384717.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            770..1468
                     /locus_tag="ACGE36_002364"
     CDS             770..1468
                     /locus_tag="ACGE36_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFF8384718.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1646..1987
                     /locus_tag="ACGE36_002365"
     CDS             1646..1987
                     /locus_tag="ACGE36_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384719.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1980..2651
                     /locus_tag="ACGE36_002366"
     CDS             1980..2651
                     /locus_tag="ACGE36_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFF8384720.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2657..3343
                     /locus_tag="ACGE36_002367"
     CDS             2657..3343
                     /locus_tag="ACGE36_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFF8384721.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            3346..4095
                     /locus_tag="ACGE36_002368"
     CDS             3346..4095
                     /locus_tag="ACGE36_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384722.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            4107..4376
                     /locus_tag="ACGE36_002369"
     CDS             4107..4376
                     /locus_tag="ACGE36_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384723.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            4381..4545
                     /locus_tag="ACGE36_002370"
     CDS             4381..4545
                     /locus_tag="ACGE36_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384724.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            4538..4840
                     /locus_tag="ACGE36_002371"
     CDS             4538..4840
                     /locus_tag="ACGE36_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384725.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            4837..4998
                     /locus_tag="ACGE36_002372"
     CDS             4837..4998
                     /locus_tag="ACGE36_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8384726.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
BASE COUNT         1876 a          788 c         1072 g         1316 t
ORIGIN      
        1 ttaatagagc gctagtgttc agcgcaccgc taatcatcta cgctttagga ctttggggaa
       61 gcaggcaagc gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg
      121 aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag
      181 aaaagagaaa ctagaatgta tattactatt actcagttta attctttcac taatttctct
      241 attgggaagt ttgtattttt gatatcaaca attttgtgga tttgggttct atttagagta
      301 aatatcattt ttgtttggtg attaaggagt ttattaaaat taattttccc tacttcaaat
      361 gcaaggtaac aagatatagc acttttagga ggaatattta gcggtacagc ttcagagtat
      421 attctattat catcgactga tcctccttta gcaattaatt tcttaaatgg agtagctgac
      481 catctgcggt ttaagttagc ggatgaaagt tctaaattaa ctaacatagc tggttctgtt
      541 gaatagttag aaatgattac ttttgtataa atcatatctg cagcgaagta agaagcatta
      601 agttcaactt ctagttgagg gcgcattcct tttattttta tccctgttaa aaacgtactc
      661 aatataaaac ttattattga catccattga aaaattgtta agtgaaaaaa attcaaaata
      721 aacaccacca gtttttaact aaattatacc aaaaaggaga gaagaaataa tgcaagaatt
      781 agtaattttg aaaaataaag aagctgtgac tacgagctta caagtggcag aaagcttcga
      841 gaaaaaacat caacatgttt taagagatat tgatgcacta aaaaaagatg tgtccaattt
      901 tggacagatg tttgtagaag gtaatgaacc agattcatat ggcagaaatc gacgagtttt
      961 cttcattagt agagatggat ttttcttgct ggctatgggg tttacaggaa agaaagctat
     1021 ctacttcaaa caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa
     1081 tactgttcct caaacaattg aagatatgat gatctatcaa ttagaagaaa tgaaagatgt
     1141 taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga
     1201 aattaagcaa aaaggaaata tgaaagttat gggagttcta gggggaaaag aaagccgagc
     1261 ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt
     1321 ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat
     1381 tgaaatgtgg ttgccagaaa ctgcgatccg tatggaaatt gatcaactga acagacaaca
     1441 gagacttttt ggtgatgaca atgaatagag ctgaagcgct aagaataggg acggcaattg
     1501 ctaattgctg gtggaaatac tataaaccaa tcatcctaag ccaacaacat attgacaagc
     1561 aaaaagcatg gcaacaaata aaaaagtgac tccgccggca agcaaagagt cacaaagaaa
     1621 acacatcata aggagatttt agcatatgga aaaagaactt tccactctag atcaatattt
     1681 gattgatcct gattggggca agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg
     1741 aaatcttttg acgaatgaag aactagcttg ggatcaagat gatttaggca accatgtaac
     1801 tatttgggat catgtttatc ttatccatct atcgaagcat tcgaataaac ctgaatatat
     1861 ttacgtcatc gaagatggct tgattgatgc gctagaagag tacgacagag ataacttgat
     1921 tgatatctct tattacggac caggtaagaa atacattgct gaaatggagg cagaatttga
     1981 tgagtgaaat caaagggaca acgaactttg aaaaactttt tagtcgtaag ttaaataaaa
     2041 ttctcaagaa aaaaggaaat tttgattatt tatcttgggc tcacgcgtgg gagattatga
     2101 aaaagaatga tccacaggca acggtaacta ttaatgagta caaacactac agggttgttt
     2161 ctggaactca tcaagacttt cttgttgagg aatataaacc ttttcttatg gatgaaactg
     2221 ggacttatgt atctgtctca gtaacggtta aaggacacac ggaaaccgaa ttatttcctg
     2281 ttttagatta tcgaaaccaa ccagttgtta aaccaaatgc aatgcaaatc aataactcat
     2341 tgaagcgatg ctttgtgaaa gcattggctc tacacggact gggattatat gtatttcaag
     2401 gggaagatat tccaacacca cctagaatcg atacaaagaa attaaacatg ctagagacga
     2461 ttctagaagc tttcaatgag cagatgggta aagatatgac caaaacctta atcgaatatg
     2521 ttaatgagca gacagataaa ttagggctct tagctgataa cgttgaaact attgaacagt
     2581 taagctatga gcaatgtgcc ttgatggagc gagcaatagc agctaagaga aaagaattag
     2641 ataagaagtg atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa
     2701 gttcaaagga aaagacataa gcgcaaccat caatgaggaa gtgaacattg atcgactaaa
     2761 gacgatgtat gacggctacg atggtgatcg agtcattgaa attcgtttta ttgatcctag
     2821 acgtttcacc gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga
     2881 tacaggcatg ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac
     2941 agggcgcaaa ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt
     3001 agcaaatatc atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga
     3061 gattttacct gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg
     3121 catctgtggc aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa
     3181 gcgcaaagaa gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac
     3241 agagaagcac aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa
     3301 attaaaccag aaaacaatca aaaagttaaa tattggaggg taaaaatgac agaacatcga
     3361 agttattatg cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact
     3421 aagttgttat acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc
     3481 aatgagtact ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt
     3541 gatttgatta agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa
     3601 ataatcaata ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct
     3661 attgacgaaa aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa
     3721 gaatatataa gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa
     3781 tacggagagt ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa
     3841 ttccctaatg attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt
     3901 aagacttata aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa
     3961 cctaagaacg caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg
     4021 gcaatcgacc aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca
     4081 aaagcaccat tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa
     4141 tagcgaatta taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg
     4201 aagaagctgg aattgaagga attaagaatg atcgttcgag ttggagtaag ctcagatact
     4261 atttcgcact ttatatcgat acaacacaat taagaaattt agcatatacc aaattactat
     4321 ttgttgattg tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaatcag
     4381 atggacctaa agacatttac agcacagatt gaactaatgc atcaagaagc tttaagacaa
     4441 agtgtatcgt acgaagacaa gtggctcaac acgttccatg gcggacgtga gagcgcactt
     4501 gatcaagtac tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca
     4561 gcgaattatc gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga
     4621 agtgatgagg cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa
     4681 aatcgcaatt tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag
     4741 tctcacaggc ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc
     4801 taagaagaga acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa
     4861 atgcgtattc gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg
     4921 gtcaatgaaa cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc
     4981 catcgattgg aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa
     5041 ccaagtcaga at
//
LOCUS       DAXWLH010000165         5010 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXWLH010000165 DAXWLH010000000
VERSION     DAXWLH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.169"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            25..696
                     /locus_tag="ACGE36_002373"
     CDS             25..696
                     /locus_tag="ACGE36_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8384727.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACGE36_002374"
     CDS             755..1195
                     /locus_tag="ACGE36_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384728.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACGE36_002375"
     CDS             1207..1875
                     /locus_tag="ACGE36_002375"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8384729.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACGE36_002376"
     CDS             1939..2562
                     /locus_tag="ACGE36_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFF8384730.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACGE36_002377"
     CDS             2618..3004
                     /locus_tag="ACGE36_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFF8384731.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACGE36_002378"
     CDS             3217..4608
                     /locus_tag="ACGE36_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFF8384732.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1659 a          833 c         1068 g         1450 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac
//
LOCUS       DAXWLH010000166         4982 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXWLH010000166 DAXWLH010000000
VERSION     DAXWLH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.170"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..255
                     /locus_tag="ACGE36_002379"
     CDS             <1..255
                     /locus_tag="ACGE36_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8384733.1"
                     /translation="SDDLIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKD
                     YEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(2190..2855)
                     /locus_tag="ACGE36_002380"
     CDS             complement(2190..2855)
                     /locus_tag="ACGE36_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384734.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2981..>4982
                     /locus_tag="ACGE36_002381"
     CDS             2981..>4982
                     /locus_tag="ACGE36_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8384735.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFPIEREPKYLFNIFFFSFIDFILSVVFILSTE"
BASE COUNT         1617 a          767 c          877 g         1721 t
ORIGIN      
        1 agtgatgatt tgattcatgt cttttccaat gagtggtttg tttctgagaa agaattacat
       61 gcttctaatc tgcaatatct accaggtgaa gatccaattc ctaatatgaa agcaattatc
      121 aacagtaagg attacgaagg atataaagcc aaacatccag aagcgaagcc atttaaatac
      181 ccacaagaaa tgaagcgagc atggcgtaaa atgttagatg atgagttgat tccacttgag
      241 aatgaattga ggtaaacgag aagagttaag aaaattgtac gattcaaaga tggtagtcaa
      301 gtggaagtgt agaaaatagg gatagatgtc ttttatttgt tcacatttta taacgaagta
      361 tttaagacag ctattgaata atgtttcctc cacctttttc attcatttac cccaccccgt
      421 tgttatatga ccgtgttttg agaggaacca ataaaaagca taggctaact aggggttcct
      481 ttttcttttg tttttttgga atccgcttaa gtgaaatgta tctgaaatga aacggagata
      541 aagtgatttt ttgctataat gacaaggttt tagctgtttc agataccatg taacccaaaa
      601 tagtttattg agaaaatagg agagtatttg atactaacta gtaggaaaaa atgagataaa
      661 aattaaatgg aatcatttca ggtaatctca aataaaaaaa tatacagtcc tgtggcactt
      721 tacctatggg actgtatatt ttttactctc agcgggcttg ttttgactcc gtgtctgact
      781 ccgtcgtatt gattttaaat gatattcgat gaccatttaa caggtcattt ttgaaatttt
      841 tgtctctata tgactcagtt tgataattat tgatagatca atttgggtct atatcgaaca
      901 agggaataat ccaataaaaa atgatttaga atatcaaaat aacaaaaaag aggactacct
      961 gtattcgtcc tcttttttga ccagattttg accagtctaa tttacattct aatatattca
     1021 tatgagctaa ttttttgatt aaatggtaag tttttccttg tttgttttag tttaaactag
     1081 tgtgtaattt aaaaatctat atggaagggt ctgtggggta atacttaaat aaaggctagg
     1141 aacctgataa catcaaggtt cctagccttt ttataataaa atgaagcgaa aataatttct
     1201 tttaaagagc cgagattatc agctatataa gagtgaattt gatgcaaaaa acagagccga
     1261 aacagtcttg gtgttcgtag ctctgcttat atgtttcgaa tttaagacaa agagttggca
     1321 aaagatgttg ataaggaaga taagataact gtgttatata aactaggtaa ctaactgtaa
     1381 caagctaatt ttaagagatt cttattagaa gacgtttagg aatataacta agaaaatgat
     1441 tatacaggta attgtcattt caataatgtt ttctaatttt cgcgtctttg ttttaagaaa
     1501 aaggccagaa tcagttggaa aaaagttcgt acgatagtga agaggggcaa aatttcctac
     1561 taatattccc actaacaaga gataaaacgg ccaggttggt gtgattcctc tataaaaaaa
     1621 tgcccatgct accgaaatcc catagattaa caactgaaca atttttacaa tgaaaaaatt
     1681 gttctgccat tactcaaagt ctcttcctcc ttgtatcgtt tatttgtttc attttatcat
     1741 tatgataggc gcttgaacaa tccattttgt attttattga gtactcttgg ataaactacc
     1801 agcgattatc atatttattt atgttactgt aagccatcat ataaaagtaa atgagcagat
     1861 catagaaata taaaaaagaa ctaaaattga tctaaattat gtgcagcttg actacgtacc
     1921 aatctatatt acttactaga ttggtcgtta atctattcgg atcgaatgag caaaatattt
     1981 ttgtgaatgt ccatccttga ttatgattcc aaaaagaatc attgggaggc tgtggcgtaa
     2041 atattatact ataagccaga aatacctatc attctgaact tttttattta ggaactttca
     2101 tctcaaggct ttttcaatta atttgtaccc ctgttttttg gataagatca tgtgcattga
     2161 ttttttctat ctctacgttc atctcctact taatgcctat ctggctcctg agggcttctt
     2221 tttaaggcgt cgtcatagtc ttggctgagg atttcttggt aatctgaggg aatggcttcc
     2281 caagtgagca ggcgaacgtt catgacgtgt tttcctttgt gtttgattac agcatggttt
     2341 ttctcttttt ctacctctcc agcgtgaaaa gacagccatg taccatggcc aaagatatca
     2401 ttacataccc aacgttcgat ggaattgtcc acgtatccta cagggagtgt ggtatagccg
     2461 atttgtggtt tcaaaaaagg attgaatcga tcgatcgttt catttttgta ttgataattg
     2521 ataagaaaaa gatagaccaa acaaaagacg agcagaaaga aagatagtgt ctttttgagt
     2581 gagaagggtt gttttgatga ataagatttc ttgttaacaa gaggttcatc tggcaatgtt
     2641 ggttctattg attcggtagc tgattcaata gaaggaaaat caatcggcag acctaaagta
     2701 tgatgtaaaa gtgacagcat ttcaaagtct ggcattgtac gagaattttc ccaactagaa
     2761 atcgttttat cggaaacacc aattttttcg cctaattctt tttgcgtaag cttacaattt
     2821 ctgcggtaag ttcgtatttt ttctccggtg ttcatcttct tctcctttcg attctacagt
     2881 atatagattt attctacatg acctatacac ttgatagata cgtttttttg atgaaaaacg
     2941 gtatgatttt atcaaagaat catttaagta agggagagtg gtgataatca aaaaaaagtt
     3001 cacagtttta ctcacaatca tagtgaccgg tatcatgagt ggttttgcct tggttagtat
     3061 aaataaacag gtactctatc ggacaaatga atttttccat caaacagata cttatactcc
     3121 gattcgattg ccacaacatt ttattgggca agaagaatat gagcagctaa tcatggtatt
     3181 tgaaaatgtc tcatcagaaa ctgagctttc ttatatgaaa aaaacagtaa atgaatccaa
     3241 tgaagtagat acatcttgga acttcaagat acagccaaag cttgaaatga cattgtatta
     3301 tcaaggggat gtcaaatctt ctcacgagaa ggtaccctat tctaatacct cctttcgcgt
     3361 aacgtccatt gaagaatcga tcaaaataaa agattttgaa ggtgaatttt tcttgatgac
     3421 gagagaaaaa gaagcctatg aacgggtcat ttcactattc actgatcgat tcaatcaagc
     3481 atttcacact gattatgcca ctaatatttt tgaagacttt gaaaatagct attcgtattc
     3541 ggttatttca aatacagacg tatataatta tcgagtgtat ttaactattg gattgattct
     3601 tttattcctt ttgttatcat cctttttctt attgatgaat aaagaaatca gtttattgtt
     3661 gaataatgac ttttcattac atcaagtatt gtggtgttta ttggggaaaa agaaaatcat
     3721 tattacattc ataattgcag gaatcattgg tggttttatt tttatgtatt ataaggagca
     3781 atttctttat ttgttccaga tgtacgtttt cttgttcagc ggaatatatg gtatctcttt
     3841 gatttctctt ttattagttg aaaaaatcgc ttcgttaaga gaagcgttca actatatcca
     3901 atacattctt tttacatttg gattgttgtt ttctcttctt ttgaccgttg ccaatgtcga
     3961 attagcaact atcgtttttt cttcttcaca gttagctgtt tcccatgaac caccaaaaga
     4021 aatcgctggt gaggactatc atgtatttta tccagtaacc atcgggaaaa accacgtgga
     4081 ttttatttat tccaatcgtt ttgcatcagc agcagatcag gaattatatg aaactttgaa
     4141 taaaaacgga agtatattag tcaatgtatc agattacttg aatgaagaaa atgaacagtt
     4201 tggcagaggg ataaaaataa atctgaatta tcttaagaaa tttcctttga ttgatgtctc
     4261 tggaaggttg atccaaataa ccgaaaaaga aacgcaccct gttatattga tccctgaacg
     4321 atatagacat acagatttaa aaaatgacct ggcgcaaata acagaatatt atcaagaaat
     4381 cagtgaaaaa gaaccgaaat ttctatttat aaaaaacaat caacccattt atacatttat
     4441 tccttctatc ccctggatcg aacattatcc ggttgttgaa atactgacat tgaaaaacag
     4501 tatgtatttg gaaagaaata ttctcagcgg agaaatctat ccaccgttaa agatcaagat
     4561 aggctctttg tcgaaagaaa ggttatttga gctgattgaa gaaagtcagt tacgtgataa
     4621 cttgcagtta tcatttccgt atactcagac tgaagagata gctgtcaaag tgttaagcag
     4681 atcatttcat tacttgatac agatattttt actcactttc ttttctttct tcttgttgag
     4741 ctatgtcatg ttatgcatat attttgtcta caattgtcgg aaaatcgcta tctttcgttc
     4801 aagtggttat tcgctctttg aaacgtataa agatttcttc atgatgaacc tcatcaaatg
     4861 gggaacgacg agtgtaatct tcctttttcc tatagaaaga gagcctaaat atctttttaa
     4921 tatttttttc tttagcttca ttgattttat tctttcagtt gtttttattt tgtctacaga
     4981 aa
//
LOCUS       DAXWLH010000167         4967 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXWLH010000167 DAXWLH010000000
VERSION     DAXWLH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.171"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            551..2152
                     /locus_tag="ACGE36_002382"
     CDS             551..2152
                     /locus_tag="ACGE36_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFF8384736.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTLILQ
                     TSRGRYGSTKIATSKFVEAKILSTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            2386..2685
                     /locus_tag="ACGE36_002383"
     CDS             2386..2685
                     /locus_tag="ACGE36_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8384737.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            2820..3197
                     /locus_tag="ACGE36_002384"
     CDS             2820..3197
                     /locus_tag="ACGE36_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFF8384738.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLTC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            3246..>4967
                     /locus_tag="ACGE36_002385"
     CDS             3246..>4967
                     /locus_tag="ACGE36_002385"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFF8384739.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGNYTQEA"
BASE COUNT         1872 a          629 c          930 g         1536 t
ORIGIN      
        1 actgaatcag gatgtattct aatttggaaa ctgccttttc ccaaagacga aacaacactg
       61 aaaaaaataa atactttaaa aataagatat tgcattcatg aaattattga taaaaatact
      121 tatgttataa atcaagtaaa agtgatgggg taaaaatctt ttaaaaaaaa ctcttgttaa
      181 aagtaattct attagcttat aatgttggta ttagttctga agccaaagga acactaaaaa
      241 attctagtag aaaagttagt tacagactct ctactatgta tgaatgtaac gaaaaaaata
      301 gcgaaaaaga taattaaata agactaccaa tcttatttaa tggaacttct gtagtaagaa
      361 aagcgatttg gcaatccacg aagtccacca agatgagcta ccaactcatt ttaatttttg
      421 cacgtttaaa gtttagcatt taagtgctta ctctaaagac atagtgtaat tttaggactc
      481 cttattacgg aatctaaagt taccctatgt cttttttgct attcaaaatt gatagcaagg
      541 agaaagtgca atgttgaata gttgcgagta tgcggacgtt ttagctaaaa ttcttaaaaa
      601 tggaattaaa cagtttaata gcaaaaatct agttgctttt aaaaagaata gcaataagat
      661 tggtgctatt gccggattta cttctaaaac aaacatgata aagactaaag gtcttatata
      721 tcgttctatt gatcgtgtct tggaagattc agatgtatta acacactgga ctcctaacgt
      781 gtattcatgg cttggtggcg gtgaaaacaa aacaattttt ggtcacaaag aagctaactt
      841 aattcagatt aatacttttg tagcagatat tgactatcca gtaggagaaa agaaaacgga
      901 tttgaattta ctagttcttt atctattgga agaagggtta ttgcctacac tgattgtaga
      961 tacgccaaaa ggttatcatt gctattttct gattcaaaac tataaccaag aaactgattc
     1021 atttgataag gcaagctata tttctaattc aaatgatttt aaatctttga gagttgctaa
     1081 aagaatatca gaaaatatca gaaaatctat ccaaaatcgt ttaccacaag tcgatatggg
     1141 ctgtaatcat tttggtattt ttagatttcc aactaaaaaa aatattgtac attatgaacc
     1201 taatttcgta gatacgtttg aagggtattt gaaatggtca aaagactttg aagaaaaaga
     1261 atatgcttta aagaggtcta acttgtcatt attagctcct aattctactg gcaagaaagg
     1321 gtctaaacga caaattgata ctaaatggta cgacttttta attcatacgg atattgctga
     1381 aggagataga aataaaacta tttttacctt agccctggca tgtaaatcaa gtggcttatc
     1441 tattggcgag tgtatggatc aattggatca acttcaattc aataatgatt tgtcctattc
     1501 tgaagttgag agaacaataa gaagtgctta tcgtggcgat tacaaaggtg ctaaaagttc
     1561 atatgtcaat gaaattatcg aaaattatgc tacacctacc gagtttgaaa aatactcaat
     1621 aaatgtaaat agacaagctg ttaaacgtaa agggaatcgt gtcgatccgt ctaattgggt
     1681 gaaatttgct aaacctagag aagaacgaaa atatagtcat tttgaagaaa gtaaagctga
     1741 tttattagct tacttagaaa gaaaacaaag atcattgaaa aaaaatgagt tgtttttaag
     1801 tgtctctatg ggaactatat ctgaagaaac acaaattcct gtatctacgt taaaggtgat
     1861 tttaaaagag cttagaacgg cgaatacatt gatattacaa acgtctcgtg gtcggtatgg
     1921 atcgactaaa atagcaacaa gcaaatttgt ggaagctaaa atattaagta cagttattca
     1981 gaataaaaaa gataaacaag tatttgtctt agaattatta ggaacagcaa acagccacgt
     2041 agaaacgatt ttaagacgtt atgaagacaa aacagtaccg ttccatgcga acatactgaa
     2101 agaaagagaa aagagcaaac atgcgccaat tagaggagct aatacaggtt agagttctat
     2161 ttattttttt gatttttaaa tagctaaatt gccaattaac atatctagtg tttctgccca
     2221 ctcctattcc tgttttaacg tatactaatg attagtagtt agtactctct tttatgtatg
     2281 gagtgtatag aactattttt acttgtgtct tttcccttaa tccacgtgta cggaatacag
     2341 cttataactg cgtatgcagt tatgtttgaa ggaggaagat tttctgtggt aattaagaaa
     2401 tatgtttact cacacccgat caatgtattt attatcggta agctaggttt atctgttgag
     2461 gtgttttgtg agttatatgg atttaaacaa ggaactctat cgtcttggat tactagagag
     2521 cgtgaggtag ctgatttgcc tatttctttt ttgtatgctt tgtctctagc ttcaggaggg
     2581 aatatgaatg aggtttatga ttgcttgtgt gagtatgaaa aagagtataa agagtatttg
     2641 aaaatttcag ccccaaaacg gaataaaaaa tatattgaag attagagaaa gaagaaaaga
     2701 ataatttgtt ttttgactac aagtagactg atagctactc tgttttttga tagtttgata
     2761 ttggctgttt ttttgttaga attaatatat atcaggtaaa taaaaaaagg gggctattaa
     2821 tgaatattga tgggatttat aatgaatcag attttaaaaa aggcatagta gatattgata
     2881 gaaaagagta tttatttcct gatgaaatag gtttatttaa aattgtaggt gaaactgtct
     2941 gtaggtattg ggggaagaaa ggaaatataa ttttacttgt tgatacgatt gagggaagaa
     3001 agttaaaatt ttcgttttgg agtaatacaa aatattgttg tgaacctgta cctaaaaggg
     3061 aagtagatgt ggctaaagaa agtgtacgat ttttacctga tcgatcaaaa ataactttaa
     3121 cttgtgaacg aacaagtaat gctaaattta caagaattag agatatatta ctttcgacta
     3181 caagtagtca gtcatagagt aagtactttt ggttaaaaat caattgaaga aagtggagga
     3241 agtatatggc agtagttata ttagcagaaa aaccagatca aggagcaaaa tttgcttcag
     3301 cattggccgg aaaaaaatta tcgaaaaaga gtgggaagta tgaatttaaa agtgaaatat
     3361 ttggagatac tattgttact tggggaatag gtcatttggt aggattatca ttacctgaaa
     3421 agtatgagtg gctaccgaat aaagataaat ggcatttaga taatttgcct tttctaccta
     3481 aattcaatga attaaagtat gaagtctcta aaggaaaaaa ccaacaattt tcaatagtga
     3541 agtcatgttt agaaaatgca gatgagataa taattgctac tgatccggat agagaaggag
     3601 aaaatattgc ttataatatt ttcaaattat gcaagcagtc gatttttaat aaaccgataa
     3661 aaagattatg gattaattct ttggttcaaa aagaaattat caaaggattt aaaaatctgc
     3721 gaaattctga agaaacgatc tcttacttca aagaagctaa tgctagacaa attgctgact
     3781 atctagtagg gatgaattat acagagttgt attcattaaa gttgcaagaa aaaggtatat
     3841 cgggtgtggt atcactcggt cgagttcaga caccggtaaa cacacttgtg gttgaaaacg
     3901 atttggcaat tagaaatttt agaccggaaa aatataaaac gattgaatgt catacaaccg
     3961 ataagcaacc aaaagcaata tttaagaata gtcttgaata ctttaaatta gaaaaatttg
     4021 aagaagacac taaaaaattc ggccttgatt ccgccagtac tggaatcata aaaaaaattg
     4081 aggttagtaa aaagcaaaca gaaccacaaa aattgtttag cttgggtggt attcaagaat
     4141 acgctaataa aaaatggaaa tattctgcta aaaaaacaga taaaattatc caaagtctat
     4201 atgatcgtgg atatttatct tatccaagaa ccgatagtga attgataact acaaatgaat
     4261 ttgagtattt aaaagcgaat ttagacgtgt ataagagtgt tttagggatc agtattgata
     4321 caccttgttt agagcctaaa aagcgatttg tagataatga caaagtatta gagcattatg
     4381 cactaatacc aacaatggat attcctgata tagcaaatct gacattagat gaaaaaaata
     4441 tttatgagat cgttactagg cgtgcgtgtc taatgtttgc agaaccttac aagtacgaaa
     4501 atacaaaagt cacattagat gtaaacggct tagaatttac cacgagtggt aatgtaccta
     4561 tatctttagg ttggaagcaa gtagatacta atttagatga atctgatgat aaagaggaaa
     4621 cattacctaa atttcatgaa caagagacaa taaatataga tattagattt attgataaat
     4681 taactaagga acctaaaagg ttgactgaag gtaaattatt aggtaaaacc ggtattatgg
     4741 cgaaattagg gctaggaacg cccgctacac gttctaatat tgtagacact cttctaaata
     4801 gggaatatat taagatagaa aacacgaatg tttatcctac tccaaaggga tatttattat
     4861 gggatttgac aaagaataaa gacctattga ttggaaaacc agaaaataca gcgatatggg
     4921 aagaatcact tgggaaaatt gcgcaaggta actatacaca ggaagct
//
LOCUS       DAXWLH010000168         4959 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXWLH010000168 DAXWLH010000000
VERSION     DAXWLH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.172"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            96..809
                     /gene="treR"
                     /locus_tag="ACGE36_002386"
     CDS             96..809
                     /gene="treR"
                     /locus_tag="ACGE36_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFF8384740.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            886..1332
                     /gene="msrB"
                     /locus_tag="ACGE36_002387"
     CDS             886..1332
                     /gene="msrB"
                     /locus_tag="ACGE36_002387"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8384741.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE36_002388"
     CDS             1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE36_002388"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFF8384742.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE36_002389"
     CDS             2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE36_002389"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFF8384743.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3147..3725
                     /locus_tag="ACGE36_002390"
     CDS             3147..3725
                     /locus_tag="ACGE36_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8384744.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3777..4259
                     /locus_tag="ACGE36_002391"
     CDS             3777..4259
                     /locus_tag="ACGE36_002391"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFF8384745.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4376..4750
                     /locus_tag="ACGE36_002392"
     CDS             4376..4750
                     /locus_tag="ACGE36_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384746.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         1702 a          850 c         1082 g         1325 t
ORIGIN      
        1 ttaaaataag cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt
       61 gaagaaatga tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt
      121 tagatttgga acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg
      181 agaaccaatt gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc
      241 ttttgactac ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc
      301 ggaatcgctt cgactttcct atttctggat tgactagcta taaagagcta caggagaccc
      361 aacgtattcc aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat
      421 tgaacgagat cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa
      481 aaatcaatgg cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta
      541 ttttaactcc agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt
      601 tgacgattgc ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa
      661 cagcgatgga tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg
      721 aagatacccg ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg
      781 ttgagtttgc ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc
      841 tgtccacttt ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata
      901 agaccagaaa aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa
      961 gaaaatgcaa cagaacgacc atttagcggg aaatatgatg atttttacga aaaggggatc
     1021 tatgtagata tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt
     1081 tgtggctggc catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat
     1141 ttttctcatg gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga
     1201 catgtgttta ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc
     1261 tcactgaaat tcattccata tgaaaagatg gatgaattag gttatgggga attcaaatca
     1321 cttgtagaat aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc
     1381 gctctttttt tattcaagcg attgctattt gaaagaaacg ctccccagac tccttttttc
     1441 acaaggtgaa atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa
     1501 tatgtatgaa tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga
     1561 aaccaatggt atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact
     1621 aaatcaaaaa ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta
     1681 tggatttgat tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat
     1741 cggtccgaaa agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc
     1801 agtagaatcc agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc
     1861 acaacaaatg atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt
     1921 tagtctattt gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc
     1981 tctctcggct cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga
     2041 agtagataat cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa
     2101 ataaggagga gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga
     2161 actgagtttg gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa
     2221 agtcaaacaa gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga
     2281 tcatgttctg ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc
     2341 taatgagatg accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga
     2401 cttagtagca atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca
     2461 tcgattacct agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga
     2521 tatcatggta ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac
     2581 tttgattggc gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg
     2641 catcatttct catatggaat attatgacga acaggatctg caggagatcg ttttacgttc
     2701 ttcggatatt tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc
     2761 acgaggaacg cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt
     2821 ccaaggagac gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt
     2881 cgatcaggct ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata
     2941 tggcggcgga ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac
     3001 ggtagaagat atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag
     3061 agggcgaatt gctactgctt atgcctatga acattttggt tacgactatc atatatgatt
     3121 tttgctttta gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca
     3181 aaaacgtatt gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa
     3241 tcgcaaatga agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg
     3301 atttgcttcg ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt
     3361 cacttgataa ttgggaagag caagcattga aagtgctgaa agcaattgct gagaagaaag
     3421 aattttatgc aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac
     3481 tgatccaaaa atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg
     3541 caagagacaa actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca
     3601 attggatcac gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt
     3661 tagccaagga tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac
     3721 agtaatagaa aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga
     3781 caaaaatttt atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc
     3841 tgagaaaacg aatagcaata gaaggaaggg aagcagaatt ttttgtggat tctgctgcta
     3901 caagcacgta tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag
     3961 aaaaagtaga tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt
     4021 ttgattggat cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac
     4081 gctcagcgca ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa
     4141 atgttcccga cccatactat accaataact ttgatgagac atatcacttg atcacagaag
     4201 gcctgaatca ttggtggaat atttggattg acgaaaataa aataacaaac aatttgtaaa
     4261 gttgacgctt tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta
     4321 ggtaaaataa aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa
     4381 cgaagagaca gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca
     4441 actttttgat tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg
     4501 aaagtttatt cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt
     4561 aagagcgaat cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac
     4621 ggtcgaaaaa gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt
     4681 tcctgtagaa caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacgatt
     4741 atcaaaataa tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact
     4801 aatcaaaaat aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag
     4861 tatcacagca tgacagcatt gggttgatta gttgaatcga ttggatggta caagtttttt
     4921 tgataaaaaa aggtcctgct agccggggag ctagcagga
//
LOCUS       DAXWLH010000169         4945 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXWLH010000169 DAXWLH010000000
VERSION     DAXWLH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.173"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(44..817)
                     /locus_tag="ACGE36_002393"
     CDS             complement(44..817)
                     /locus_tag="ACGE36_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8384747.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            961..1311
                     /locus_tag="ACGE36_002394"
     CDS             961..1311
                     /locus_tag="ACGE36_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFF8384748.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1292..2008
                     /locus_tag="ACGE36_002395"
     CDS             1292..2008
                     /locus_tag="ACGE36_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFF8384749.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2008..3456
                     /locus_tag="ACGE36_002396"
     CDS             2008..3456
                     /locus_tag="ACGE36_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8384750.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3526..4035)
                     /locus_tag="ACGE36_002397"
     CDS             complement(3526..4035)
                     /locus_tag="ACGE36_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384751.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4025..4750)
                     /locus_tag="ACGE36_002398"
     CDS             complement(4025..4750)
                     /locus_tag="ACGE36_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384752.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1695 a          843 c          997 g         1410 t
ORIGIN      
        1 taagcgattt tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc agatcaaaat
       61 acttcaatgc gtggacaatt ccgccttctg tatttttctt tgtgataaag gtagaaagtt
      121 cttttaatcc gtcataggcg ttgtccatag cgattttgtt gtcgcaagct tccaacaagg
      181 caaaatcatt cggaccatcg ccaaatccaa aagtaggaac tttttccagc tgaagttctt
      241 tttgcagacg tttgacacct gccccttttg agattccttt cttcactgta tcgatcgagt
      301 aaggaccatt ccgataaaaa gttagctcag gaaaatgttc gtgatagtat tcgtcacctg
      361 attgagataa gatcagcaac atatttactg tgttttcttt aaagcctaaa ggatcaattt
      421 ccggaacatc tgaatgaatg aagtcgtaag cattgatcat tgtttggcta tgaccggtac
      481 accagatttt ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt acatgatcat
      541 acattttttg acattcttct gaagaaaatg tttgagaata gatttcttcg ccgtctactc
      601 gaatgaaaga accattcatg gtgaccgcag aagtaatgcc cgcatcttcc atgatagagt
      661 ggatttctgc ttctgtacgt cctgtagcga tgacggggag aacattgttt tcttttaatg
      721 cgttcatagc ttttgccact tcgggtgtga tttttgattt cgcatctagt aatgttccat
      781 caagatcaaa gaaagtgatt gcacgataat ttgacacaaa taaaactcct tttttattgg
      841 aataagttta tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag aattcctgaa
      901 catattggtt cagtcaacaa tttagagtat actataagaa gagaatttag gagggaaaga
      961 atgatttttt tcagtattgg tatcttattc ttaataattg gcagtctttt tcggattctg
     1021 ccctcaaaag gaaatctgcc tttttatggg tatcattcgc ctcttgccgc aaaaacagac
     1081 gctcattggc ggctggctca aaagacaagc ggaaactggt tttttcttat gggcttactg
     1141 atggcattga tcggctatta tttaaagaca tctgggcata cgaattattt tctgattgaa
     1201 atgcttctgc tagtatttcc aattatgcct atttttatta tgacagaaaa gaaattacag
     1261 aaatatgacc tggaaactgg agggaacgat aatgaatatt ttaatgattg aagataatca
     1321 atctgtttct gaaatgatgc agatgttttt cttaaatgaa ggttgggaag caactttcaa
     1381 gtatgatgga aaagaagggc tcgatgcctt tttagaaaac ccacaacatt gggatatgat
     1441 tacactagac ctgaatttgc ccacactgga cggtatgcta gtaggaagag agatccgcaa
     1501 agtttcaagt actgtgccga ttatcatgct gacagctaga gattctgaaa gcgatcaggt
     1561 gatcggctta gagatggggg cagatgatta tgtcacaaaa cctttcagtc ctctgacgct
     1621 tattgctcga atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa gagaagaagc
     1681 agcagtttca gatgatcatt ttgatatcga gactagtcat ttcaagatga atacgaaaac
     1741 acgagaagcg tatctggaca accggccaat tgaaggactg acgcctaaag agttcgatct
     1801 attgtttact ttagcaaaaa aaccacgtca agtattctct cgggagcagt tgcttgaatt
     1861 agtctgggat tatcagtact ttggtgatga acggacagtg gacgcccata tcaagaaatt
     1921 acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa actgtttggg gagtcggcta
     1981 caaattcgat gattctggtg ttgcctaatg cgttatttgt atcagcaact cttagctttt
     2041 tgcggaatgg tcgcgttgat cttattgatc gttggagttt cttttacaca actgacaaaa
     2101 caaacgattg aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc agttgaaaag
     2161 acaacgcaaa cctatgccga tacgctcccg cagtttagtc aagatcaggc gtttcaaaat
     2221 gcattgctgc tgacagagca agtactgaca gaacaaaatg tgaactttat atttatcgat
     2281 aaaaacgaac gtgtcattta tccgactaat acagctttac tgaattttac cttaacagct
     2341 gatcaatgga aaaatctgag gaatggacgt caagaaaaat tcacatcgaa taaaaatatt
     2401 ttaggacaag cacaggcgac ctcttacgca cttgtgccat ttaatttgaa tcatgaattc
     2461 tatggagcac ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt tcgtccaatt
     2521 acactgaacc tgttcaaagg atttattttt tccagtatcg ttgcagttat tgccagctat
     2581 gcttttgcag cattccaagt taagcgtatc aatcggttaa ggaatgcgac gaaagaagtg
     2641 acgaatggta attttgatgt acagctcccc gtacatgaca aagatgaatt tgatgagtta
     2701 gcagatgatt tcaataaaat gacgaattca ttgaaagaat ctcaagcaga aattgaagaa
     2761 caagaaaatc gtcgaagaca atttatggca gatgcttctc acgagatgag aacacctctt
     2821 acgacaatca acggacttct ggaagggtta gaatacaatg caatccctga aaatcagcga
     2881 gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt ggtgaatgaa
     2941 aacttggatt atgaaaaaat ccgtacgaat caaatcagta tggtcattaa aaaattcaat
     3001 ggaacagaaa cattgaaaaa tattgttgca cagttagaag ctaaagcaga agcagcagga
     3061 gatacattga cattgaaagc cgttgatgat attgatgttt acgctgatta tgaccgattt
     3121 gtccaaatca tggtcaatat tattcaaaac gcgatacagt ttactgaaaa cggccaaatc
     3181 acgattactt tagaaaaagg ctatcttgaa acgatcatca cgattgaaga tacgggaatt
     3241 ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt attataaagt agatccttca
     3301 agaaaaaata caaaatatgg tgaatcagga ttaggtttac caatcgtgca gcaacttgta
     3361 cgcttgcata aaggaaaatt agaagtagaa agcgaactag gaaaaggaac aaaattcgct
     3421 gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat aaaagatcat
     3481 gaatcgatct tctataccgc ttgtttttac tataaaatga ttgatttagc ccgcgggctc
     3541 taaacgatca atataaggac tttgagcaat agtagcatca tgagtaaaga gtaagatacc
     3601 ttgattttct tcatgagcta aataagaaag caagtggagt agctcttgga tctccaatac
     3661 actaagttca tcaaatgtat cttcaataac aatattgttt cgcttggaaa gcaaggcatg
     3721 aagtaattgc agctttactt tttcaaaaga cgtcaactca gctgccgcac aattaagcaa
     3781 ggaaggagag atatcaagtt tttcttgata ggccaaacga tcttgtttga tttctttttc
     3841 cggtacatcc aacaggacat ggtctttaag attcagataa ggaaacatcg gccaattttt
     3901 ttggatgatc gcaaaattat ccagttttgc aacaagaacc tcttttttca agtgctccaa
     3961 tattttatgc tgtttttcga gaggacacaa aataacggtc aagtgttcag catgaatcaa
     4021 atattcaggt ttcattacgt ccaggcctcc tactttgcaa atgatggata aaaaaagtga
     4081 aagggaatag actaagaatg agacagcatc cactgagccc aataagagtt tgggcaaatc
     4141 ccttaagtat atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa aaagtctgtt
     4201 gatccatcgg aattgtaagt ccgcttaccg aagtcaagat atcacggata tctggcagtt
     4261 cagtagtctt taagacaaga aaattgatcc tttgaagaag cgcttcataa actgactgga
     4321 agctgacgat ccacaataaa accaagatac aacaaagaaa gagaggaaag aacagctgcc
     4381 agaaaataaa aaggaataat ttggttttag aaagaccagt caggcgccaa ttgatcaatt
     4441 cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag gcaaatagaa
     4501 aaaataaact gaatatgcct tttatataaa atcggctagc taattctaaa gccggaagaa
     4561 gctctgtaga tgcagaagcg ccaataaatg aaagccgatc ttggacttga ttggctaaag
     4621 ccgattgcga atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa aagcttgaaa
     4681 ataaacaata gagtattgtc tgctttttgt aataaattaa tcgagcagta gcatagcgga
     4741 ttattttcat acttttcacc tctataaaaa gtatagaaaa tgaatatgaa taaagtatga
     4801 aattccttta gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa gcaagaggtt
     4861 gtgaagaagc tgccctattc tattaccgta gatgcaagct tttgaacact gtttattcga
     4921 aaagaggagc agcgacaata atttt
//
LOCUS       DAXWLH010000170         4925 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXWLH010000170 DAXWLH010000000
VERSION     DAXWLH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.174"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(56..880)
                     /locus_tag="ACGE36_002399"
     CDS             complement(56..880)
                     /locus_tag="ACGE36_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8384753.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(877..1632)
                     /locus_tag="ACGE36_002400"
     CDS             complement(877..1632)
                     /locus_tag="ACGE36_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8384754.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(1645..3621)
                     /locus_tag="ACGE36_002401"
     CDS             complement(1645..3621)
                     /locus_tag="ACGE36_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8384755.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(3674..4345)
                     /locus_tag="ACGE36_002402"
     CDS             complement(3674..4345)
                     /locus_tag="ACGE36_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8384756.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(4405..4614)
                     /locus_tag="ACGE36_002403"
     CDS             complement(4405..4614)
                     /locus_tag="ACGE36_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384757.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(4681..4920)
                     /locus_tag="ACGE36_002404"
     CDS             complement(4681..4920)
                     /locus_tag="ACGE36_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384758.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1326 a         1033 c          737 g         1829 t
ORIGIN      
        1 cggctaaaaa ggatagatta aaaactaata ttcttattat tttcatcatt tcatcctaat
       61 tatcaaaaaa taattgagca aaaaattccc aacaattttc aaaaaagttc ttctttacac
      121 cagttgacac aactttttta atttcttctt tcggctctga tttctctgat ttagggtata
      181 accaaacatc cgtcaaaaat tcaccttttt catttgaaac aggtgtcaat aaaacaatat
      241 cttcactgtt tgaaatgttt tcagggcttg attcattaac gatatagtat gcttgttttt
      301 tgtttgcagg tacttcaatg gaaaattcaa tcacgccttc ctgatcatca atcgttttgg
      361 tttctccttc agccactttt ttcaactggt cgtatgaaag tttttttgct cgatctttca
      421 attttgactc aattatttct acagattgat ttttctcatt atcatcttgt gacatttctt
      481 ctaagatatt cgtgatatcg tagacaataa acttcgcgcc attgatacct tcttgctggg
      541 ctacttgact gccttgattg ttgatttgat tcgaagaaat aattttttga atatgaatgg
      601 cttgcttgat cacttgcgta tcttctacgt cttgcggctt ttctgaagtc tctgaagaac
      661 tttctgacgt tggattcgtt gattcagtaa agcttatatt tgaatctgcc gtttcttctg
      721 tagtattctc cgaactgtcc gtttcgcttg gtgcagtaat tgaattatct attgtttgag
      781 tggtactact atcactgctg tcagcgaaag acagttgcga ataagctaca gtagaaaaga
      841 gagctgctac acaaacaaac agccattttc tcttattcat tccgatctcc tccttatttt
      901 tttataaata aaaataatag gaacgcaaaa aaaagcgaat agcagtgcta atcgccaatc
      961 caatgaaatt gatttcctat tttttgcttt tttctgtagt tttaaatctt ctttactgaa
     1021 ggaaactcgt tcaccataca ctaaaaagcg gtgtgagttg acaccttttg gtgtgcatgt
     1081 cattaacgtg atcctatctt ggtctttttc aggcataaga taatctaaat aatttttttg
     1141 taattcttct gctttaactt cttcaattcg ataaatttga taggcatgtt gttcttcatt
     1201 taccttaata aagaattttt gccctttttt tactgaaggc aaatcatcga aaagattatc
     1261 tttataaagt ccactgtgcc ccgcgattaa aggattttta ccgtttcctc ctgtaatatc
     1321 gccagttcct tccgtaatcc ctacaccatt ctctaaaatt ttgtcggaag ttccgtcata
     1381 aattggtagt tttaaagaaa tacttggaat tgtaagaaca ccaacaggat cgcctaactc
     1441 tattttcgca acatccaaag aagaatctgt tttttcaaga ttttcaatgg ctaaaattct
     1501 ttgctgttgt tgaaatttcg ctacatttgt ttccacaact ggaattaaat agactgctac
     1561 tgccgacagc aaccaaaaaa atatcatcag tttgtgcatg actcttcttt ttgggggctt
     1621 acgaataatc attagctgat ccttctaggc tttcttctta aagtaaacaa ctgcagaaag
     1681 tccccctgct cctagcaaca agcaagagat caatacgtaa gattggaaac cgttgctacc
     1741 agtcattggt acggaagctt tttgattatt tttgatcgtg attcggtttt cttctttgta
     1801 tgaatctttg tctactttga aagagatctc ttttgttaat ttttgatagt tttcaggagc
     1861 ttttgtttct tccagtgagt aatctccttc agttaaacca gtgatttcaa aatgaccctc
     1921 tttatctgat tttaggattg ctgcatttgc tacatcgtct acccaaactg cttctttgac
     1981 accatcttta tctgtatcag taagtccacc gtagtactta ccttctgcat tcttagctgc
     2041 aaattctgcg ccttcaagcg gtgttgcttt gatacctgtt ccaaataggc cgctatcgac
     2101 ttttaagaat tttgcgccat aagtcacaac gtcagctttc gttttttcgt ggtgttcttc
     2161 ctgattaaat ggattattgt tataaacgaa atccacgcta tttgttaatg gatttgcgat
     2221 ttcagcacta tcattcacaa acatgttata agtcattttt agatcttgac catgtaaatt
     2281 cgcaactgta tcacttactt gtccattatt tacgataaaa tcgatcacaa aaccactatc
     2341 agaattttcg attttatagt cagtattttc ttttaatacg actgaaccat cattatttgt
     2401 tactttaata tctgcttttt cagcaacata agaaagtcct ttacctgctg tatctcccca
     2461 tttaaataca ctgtaaggag aagtttcgct tttcacaccg tttactgtga taccgtctgc
     2521 gccacctgca atgtctgttg gaatatgaat cgttccttga taatttactg cgtcaccaat
     2581 ttggaaagaa tgttcttgtc ctgttccatt tgttactgtt ttatcgatca ctggtgcttt
     2641 atagtttacg tatgaagctt ttaagtcact tgtcacgcca tcattcgtaa tttcaaaggt
     2701 taatttattg tgcgcatcgt tgcgagcgtc tgcgcctaat aaatattgat ctgctgttgg
     2761 ttcttgatct gatcccacta caacttccga aggaacttcc acaaaatagt attttcctaa
     2821 cgtcaaatca gtcgcagtca gctttccttg atcatccgat acaagatcac ccaattttgt
     2881 tccttttcca ggttcgccct tgtataactc aaattttgcc ccttttaaaa gggtttgttt
     2941 gctttggtct gcgccatcgt caccaaattt cgttaattca aaactcaact tagaaacaat
     3001 gttcttagga tacaaatgaa tattttttaa aaatcctgat cctgttttat ctgtcattgg
     3061 cgcaatcaca accattggtt ttgctttttg tgtgactaat ccagcagcgg atttactttc
     3121 ataaaccaca tatacattcc ctttggaatt gacataggct ggttgatcag taaaagtgat
     3181 ctcaccattt tcatctacag ctgaagtaat tttgttggtt gcattcttga cgtactcact
     3241 gttagatccc ttgtcttcta catcttgtac aatctcgtcc actttcgcgt tggttagatc
     3301 agaatcttct ctcggtaata cttgatccgt aatatttcct aacgtaaact caacatcacc
     3361 ataatctgcc ttgttgtatt tcgttacacc tgtaggatat tgatcgtgcg tataaccatc
     3421 gtttttgatc ccgtcaatat caacattcaa ttgcgttcct tgatcgtaca ttaatttatg
     3481 aaccgtgata ttgaccttct ctggtggtgt tttttgcgtg acatcttctg catgtacttg
     3541 atacccctga aagcagcctg caatgattcc taacattcca acaagcattc cagctttttt
     3601 tacataccga tttattgcca ttttttactc tcctcatttc atatttcaac gactcgcgtt
     3661 gattaaaacc ttgttattgt agctctccct gtaccacgta tctttctttt ccttgtgacg
     3721 tataaacaca agtaaacaac gttaataagg gtttctctcc tttctttggc aaagacaaga
     3781 aagagttctc ttcaggaacg ccatcgatta atttaaagta cccaggtacg ataaatttac
     3841 gcgttacctt gtaacgatag acgccatcgt tactaactaa gtccatcacc tcccctaatg
     3901 acaacttttg aatatcagaa aataaaacac ctggcatttc catgttatga cctgctagga
     3961 catagtttcc tttgcccatt tcggcatctt cataaaaata agtagccgct ccaagactca
     4021 aagtgtattg atttgcccct ttaaaaattg gcaatgctaa accaagtgca ggaatctgaa
     4081 tttgtgcaaa tacttgttta ttgacgccca tttcgattgc ttctttttgt atggacaaca
     4141 tttgccccaa agacatatca ttattgacgt tcgttgcatc ctccttataa ctctttgcat
     4201 gtgcattgat ttgatctacg ggcatctttt tttctatatt ttgcttaaac acctctcctt
     4261 catgtttttg gtagctaaca tagccaaaca cactgcaaaa gacgattaaa gtgactccta
     4321 aaaaaccaaa taaccatttt ttcatccatc tgcctccttt cagacaaaac aaaaaaactt
     4381 tttctcattt ccatagaaaa agttttaaaa atgaattcta taagttcgtc tcaactgttc
     4441 aaagtttttc attgaaccta acaaacaagc tttctctttt cctgttaact gcatatcatt
     4501 gatatattgc gcgacagata atcttttaag gtgtttttgc ttcatcgttt ttagtaactt
     4561 ttcttccatt tctttcgaca ctttttttct gttttcgatt tcttcataat tcatgagttt
     4621 tcctaccttc cttgtttttt gcaaaaataa aaaagcctat gatcacatac gctctacact
     4681 tcagtcttgg gttatctttt tctttcgttt aattactggc cgtgctggtt tcaaatcctt
     4741 gattccatta tgggttctcc aataatcgaa ttcttttcgc aatctttctt tttccatatc
     4801 tgttccttta gataaggctt tgaatacttc tttaggtgaa taaccacgag ctaacagctg
     4861 tacatacctt ccttttgtca aagtatccat atccatcgcc tctaaaactg gtcgcgccat
     4921 ctact
//
LOCUS       DAXWLH010000171         4916 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXWLH010000171 DAXWLH010000000
VERSION     DAXWLH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.175"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            206..1480
                     /locus_tag="ACGE36_002405"
     CDS             206..1480
                     /locus_tag="ACGE36_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8384759.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1534..2961
                     /locus_tag="ACGE36_002406"
     CDS             1534..2961
                     /locus_tag="ACGE36_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8384760.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2974..3846
                     /locus_tag="ACGE36_002407"
     CDS             2974..3846
                     /locus_tag="ACGE36_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8384761.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3941..>4916
                     /locus_tag="ACGE36_002408"
     CDS             3941..>4916
                     /locus_tag="ACGE36_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8384762.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTS"
BASE COUNT         1682 a          682 c          972 g         1580 t
ORIGIN      
        1 tataaaaaat agtgccatta aaaaagaaaa gcgacacacc aataaaagtc atatcctgtg
       61 acattatatg gcattatcca aatagaataa ccggttgaca tgtaagcgga tttcttttat
      121 aattttagga gtacaagtcc ttttctaaaa atgttaaccg gttatcatga aaggggatac
      181 aaatatatcg ttggaggata aaaatatgtc aaaagttaac gtatttaaaa atcaatctta
      241 tttacaaagt tcagctacat tattactatt ttttgcttct tggggtgttt ggtggtcatt
      301 ttttcaactt tggctaacat ctgaatcaaa tggtttaggg ttatctggca gtgctgtagg
      361 aacagtattc tcggcaaatt cgttagttac cttaattttg atgtttattt atggaacatt
      421 acaagataaa ttgtatatta aacgaaattt attaattttt gcttctgtat tagcgacact
      481 tgttggacca ttttttatat ggatatatgg gccattgcta gataacaatt ttaatttagg
      541 cattattatg ggagcgctat ttttgtcagc tggatattta gcttctgtag gagtttttga
      601 agctgtgtca gaaaggttta gtcgtttatt tggctttgaa tatggacaag caagggcgtg
      661 gggatcattt ggttatgcct tggtagcgct tttggcagga tttttatttg taaaaaatcc
      721 tcatttaaac ttttgggcgg gatctttctt tggttcttta ctattgttaa atttattatt
      781 ttggaaccct aaagttgaac gggaagcaaa tcaaaatttt aatcaagaac aagctgaatc
      841 aaatagtatt ccttctttaa aagaaatgtt tgatctaatg aaactgcctc aattatggac
      901 gataatcatc tttattgttt ttacatggac attttatacg gtattcgatc aacaaatgtt
      961 tccgggattt tatactggtt tgttttcaac atcagctaat ggtgaaaaaa tatatgggac
     1021 attgaatgct attcaagtat tttgtgaagc gttaatgatg ggaattgttc caatcattat
     1081 gagaaaatta ggggttcgaa atactttgtt attaggtgta accattatgt gtgtacgaat
     1141 tggattgtgc gggtttgcct cgacaccatt atctgtttca tgcataaaaa tgttgcatgc
     1201 tttagaagta ccattattta cattaccaat gtttcgctat tttacacttc attttgatac
     1261 aaagctatca gcaaccctct atatgatagg atttcagata gctgctcaaa ttgggcaagt
     1321 gattttatca acaccattgg gaatattaag agacaacgtt ggctatcaac caacatttaa
     1381 aattatttct cttattgtat tactagcagg catatatgca ttctttattc ttaaacaaga
     1441 tgatagagat gttcaagggg atccatttat tcgaggataa tggaaaccaa cagaataaaa
     1501 aacaatttta tctagaaagt gagcgaatat attatgacaa aaagagctat agaattaaca
     1561 aatacacgtt atcgtttggg atatcatgtg tctgcaccat caggatggat taatgatcca
     1621 aatggatttt gctattttga tgggtattat catgtgtttt atcaacatca tccatatagt
     1681 gctgaatggg gaccaatgca ctgggcacat gctagaagta aggatttagt tcattgggaa
     1741 tctttaccac ttgctttgac tcctggagat caagaagatg aaggtggttg tttttcagga
     1801 agcgctattg aaaaaaatgg tgtgttgtat ctgttctata caggacatca ctattatgga
     1861 gacaatgatc cagatcattt ttggcaaaat caaaacatgg cgtatagtac ggatggcatt
     1921 cactttacta aatatgaaaa aaatcctgta atagcaaaag caccagaaga taacacacat
     1981 catttcagag atccaaaagt atggaaacac gatgaaaaat attacatggt tttgggtagt
     2041 caagaaaaag atggattagg tcgtgctatt gtttatcagt cgttagattt attaaattgg
     2101 gattacattg gaccaatttc taaagcgaag gaagtactta cagaaggatt tatgtgggaa
     2161 tgcccagact tttttgaatt aaatggaaaa tctattcttt tattatcccc tcagggaatt
     2221 gaagcaaatg gaaaagatta tttaaatcta tatgaaacag gctactttat cggtgattat
     2281 gattatgaga cagcaaaatt cattagagga aatttttatg aattagataa gggacacgat
     2341 ttctatgcaa cacaaacaac tttatctcca gatggtagac gtattgttat ggcctggatg
     2401 gatatgtggg aaagccctat gcctgaaaaa gtagacggat gggcaggagc attaacgctt
     2461 cctcgagaat tagagttaat tggagatcgt ttgtatatga aacctgtgag agaattagaa
     2521 caacttcgag ttggcagcgg cgttgaaacg acagttaatt tggcaggaga aataataatc
     2581 gctcaagatg cttcattcaa agaagctcta attgatgtac tattgactaa atcaaaaaat
     2641 caagaagtca aattttcatt aaaaacaaaa gatgaagagt taatcaatat tttttattct
     2701 aaggataaaa atgaatgtac cttaactcgt aacggcaaaa atgatccaag gtatggaacc
     2761 attcagatgt gtgatagatt atcacttcga gtgtttatcg ataaaagttc ggcagagatt
     2821 tttattaatg atggcgagct tgtttttaca gagcgttatt atacagaaaa tcaaccaagt
     2881 atgcatttga gtgttagtga agaagctttt gtctcgagta caatttatca tctaaaaaaa
     2941 gatgcaattt cttacgagta aggaggtttt atgatgttaa ttggtagcat agaagcaggt
     3001 gggactaaat ttgtatgtgc cgtgggagac gaggattacc gagtacaaga ccaaataatt
     3061 tgtccaacga ccacccccag ggaaacattg aaaaaaactg ttgactattt taaacagttt
     3121 gatataaatg cattaggtat tgcatcattt ggtccaatag aaattcgaaa aaattcacca
     3181 aaatcaggat atattacgtc tacaccaaag gtgggatggc gaaatgttaa tgtgttaggt
     3241 tatttacaag atgagctgaa tgttccaatg tcttggacaa cggatgtgaa cggatcagct
     3301 tatggagagt acatcatgtc cactttatcg aatgaaaaaa taaattcatt agtttattac
     3361 accgttggta ctggcgttgg tggtggagct gttgttgacg gaaaactatt aggatcgttt
     3421 ggtcattcag aaatgggcca tacgtttgta aaacgccatc ctgatgatat tgattttaaa
     3481 ggaatttgtc cttatcatgg tgattgttta gaaggcttag ttgcgggacc aacgtttgaa
     3541 gcacgttttg gaaaaaaggg tgaagacgtt tctttaacgg atcctatttg ggatgtaatg
     3601 gcctattatc tagcacaagc tgtgattcaa acaacattga ttatacgacc ggataaaata
     3661 gtatttggtg gaagcgtagt aaatgaaaaa ttgttagaaa aaattcgtgt taatgttaat
     3721 aaattattgg atgattacgt ggaaatacca ccgttaagta aatatattac gcgtccttta
     3781 attaaaaata atggatcggc aacactagga aactttgctt tgggcttgaa tcaattacaa
     3841 gaataaaaca tagaaaaaat atcgattatc aggtatagta gttctataga gttctttttt
     3901 tcgatagtta attattgcat tatttaggag agacaagaga atgcgcccaa aattagaaga
     3961 tgttgccaaa agagcaaatg tatcaaaaac aactgtttca cgtgtattaa acaatcgtgg
     4021 ctatttaagt caaaaaacga ttgataatgt ttataaggct atagaagaac tcaattatca
     4081 accgaacgtt gtagcgcgtc aattatttca aaagaaaact aacattgtcg ggttactatt
     4141 tcctacagta gccaatcctt tttttagtga actcgttgag gcattggaaa aaaagttata
     4201 tgaaattggt tataaggtat taataggcaa ttcgatgaat aataaagaaa aggaaacaaa
     4261 ttatttaaat cagttattgt ctgatcaagt agatggttta attgttggta cacataacca
     4321 aggaattcag gaatataaat atcagaattt accgattgtt gcaattgatc gagtgatgaa
     4381 tgaagatatt ccagttgttg aatccgataa ctacaatggt ggaaaacttg caaccaagtt
     4441 attaattgca caaggtgcaa aaaatattat tcatacaaac ggtccaattg acttacagac
     4501 tccggctaat cgtcggagac tagcctatga agatactatg aaagcatatc agttaattcc
     4561 acgaactgtg acattagatt tcaatatttc ttatgtaaag aaaaaacaga tttttttcca
     4621 aatgtttgag gattatccta aaatagatgg tatttttgct tcaaatgaca tcgatgcggc
     4681 cctaatatta caagtagcaa aagaaaaggg gttaaatgta ccagctgatt tattagtagt
     4741 cggatatgat ggtactttaa tgactcggag tatattacca gatctgacaa cagtaataca
     4801 accaattaat gatatagcag acacagctgt tgctattttg atgaaacgaa taaataaaga
     4861 agaaacaaaa aaggaatata ttttacctgt cacattatgg atggggaaga catcag
//
LOCUS       DAXWLH010000172         4894 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXWLH010000172 DAXWLH010000000
VERSION     DAXWLH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.176"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1284
                     /locus_tag="ACGE36_002409"
     CDS             <1..1284
                     /locus_tag="ACGE36_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFF8384763.1"
                     /translation="YKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTVKK
                     QNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINIDPY
                     ANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQSF
                     IEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRPSD
                     DTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRTVN
                     QKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKENPY
                     FYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMHEG
                     FDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            1297..3999
                     /locus_tag="ACGE36_002410"
     CDS             1297..3999
                     /locus_tag="ACGE36_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFF8384764.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            3984..4841
                     /locus_tag="ACGE36_002411"
     CDS             3984..4841
                     /locus_tag="ACGE36_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8384765.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
BASE COUNT         1729 a          850 c         1062 g         1253 t
ORIGIN      
        1 tataaagata taccaaaatc ggtgcagaaa tttatggaag atatcacaca aaaatgtggg
       61 gatatccatg cggactgggc aaagaatttt acagcagcat ttgccaatac tctgttgaca
      121 acagtcaaaa agcaaaatga cggaaccacc tttttattga cgggagacat tccagcgatg
      181 tggttaagag actctactgc acaagttcgt ccttatttag tgattgcaaa agaagatgag
      241 gaattagctg acatgatcag cgggttagtc aaaaagcaat ttttttatat caatatcgat
      301 ccatatgcga atgccttcaa cgaagaagct aacggtgctg gccaccaaac agatcataca
      361 aaaatgaacg attggatctg ggaaagaaaa tatgaaattg attctttatg ctacccaata
      421 cagttggcct atcttcttta caaaaataca ggaaaaactg atcaattcaa tcaatctttt
      481 attgaaggta tcaaaaaaat cttggacgtg ttcaaaacag agcaagatca tgaacactct
      541 ccttatacat ttgtacgaga cacgacaaga aaagaagata cgctgatcaa tgacgggcga
      601 ggtgcaaaaa cagcttttac aggaatgaca tggtctggtt tccgtcctag cgatgatact
      661 tgccgttatg gatatctggt accatccaat atgtttgcgg tggtcgtgtt aggttatgtg
      721 gaagaaattt ttcaaacgat actcgatgat aaagaaattg tacagcaaac acagaaattg
      781 aagaaagaga tccaagaggg gattcagaat cacggacgaa cggttaatca aaaagggcaa
      841 acgatttatg catatgaagt agatggaatg ggaaacgcca cagtcatgga tgacagtaat
      901 gtgcccaatc tgatcgccgc accttacttg ggatattgtt ctgaacaaga ggaagattat
      961 ctgcaaacaa gaagaacatt gttgagtaaa gaaaatccat atttctatga aggaaagtac
     1021 gcaaaaggaa ttggatcctc ccatacgcca gaaaaatacg tttggccgat tgcattagct
     1081 atggaaggaa tgacaacgtc agacaaatct gaaaaagagc ggatattgaa tttacttgta
     1141 gcaactgacg caggaacaca ccttatgcac gaaggattcg atgtgaacaa tcctgaaaat
     1201 tatacgagag aatggttttc ttgggcaaat atgatgtttt gcgaattagt catggattac
     1261 tttgatatta gagtagaaaa gtaggaggta tcgtaaatga aaaagaaagt atatatcatt
     1321 tctcatagcc actgggatcg tgaatggtac atggcttacg aacagcatca catgcgactg
     1381 attgaattga tagacgatct actagagcta tttgaagttg atccgtcctt caacagtttt
     1441 catttggatg ggcaaacgat tattttagat gactatcttc aagtaaggcc agaaaaaaga
     1501 caggcaatcc aacaagcaat caatgaagga aaattgagaa ttggaccatt ttatatcttg
     1561 caagatgatt ttcttatcag ctcggaatcg aacgtgagaa atatgctgat tggcatggaa
     1621 gaaagccgta aatggggaac ccctgtcatg ttaggctatt ttcccgatac atttggaaat
     1681 atgggacaga cgcctcaatt aatgaaacaa gctggtattt cagcagcagc gtttggccgt
     1741 ggtgtaaaac caattggctt tgataatcaa gttttggaag cagaaaacta ttcttcccaa
     1801 tactcagaga tgtggtggaa aggacctgat caaacagcaa tctttggtct gctatttgcc
     1861 aattggtata gcaacgggaa tgagattcca gtagaaaaag aagcagctct tgccttttgg
     1921 aagcaaaaat tagcggatgc agaacaatat gcctctacga accacttatt gatgatgaat
     1981 ggtgttgatc atcaaccggt acaaaaagat atatcaaaag caatccatct cgcaaatgaa
     2041 ctgtttccag attacgagtt tattcattct aattttactg actatctgga agcagttcaa
     2101 aaagatgtgc cagaagactt aggctcagta gaaggagaat tgacaagtca agaaacggat
     2161 ggctggtata ctttagccaa tacagcatct gctcgggttt acttaaaaca atggaatacc
     2221 aaggtccagc gacagctaga aaatattaca gaaccacttg cgacgatggc ttatgaagtt
     2281 agtggaaact atccccatga tcaattagat tatgcttgga agacattaat gcaaaatcat
     2341 cctcacgaca gtatttgcgg ttgttcagtc gattctgtcc atcgggaaat gatacctcgt
     2401 tttgaaaaag cagatgaagt tggaaagtat cttgcacaag attccttaga acagctgacc
     2461 gcagcaatcg atacgaccgg attcccaaaa gacagctttc catttgtcat tgtaaatact
     2521 gcaggaatgg ataaaactgg tgaagcagaa ataaccatcg aattagaaag aaaaagattc
     2581 gctgaaggaa tacctgaaca actatatcaa gagttagaaa atttgcctaa acgtaagtat
     2641 catgtagaaa caaaaagtgg ggccactata ccagctattt tatctgaaga aactgtccaa
     2701 tttggttatg atttgccgaa agaccgtttc cgtgtgccat atatggcaag aatgatcaaa
     2761 gtgactttac cattagaaaa catgccagca ttttcttggg aaacattcgc gcttgtagaa
     2821 ggggaagcga aagcagaaga aaaggaaaca atgattcatc agagtggacg aatcatcgaa
     2881 aacggccctc tacatttgac gattgaaaaa aatggtacca ttacaatgga agatcgcaaa
     2941 aacaaacgaa agttgaataa tctgcatatt tttgaagata tcggcgatat aggaaatgag
     3001 tatatcttta aacaaccttt ctgtgatcag ccaatcctat ccagtaacaa agaaaactct
     3061 gaagtaaaag tattagtaga tacaccggaa atagcaaaaa taagtattct acaggaaatg
     3121 gaaatccctg tttctgcaga cgagcgttta gaaaaagaac agcagatggt tgtggaattt
     3181 cgttatcgaa aagcagaacg atccaaagaa aaacgaatcc ttcaaataaa aacaatcatg
     3241 acaatcagaa aagatagtaa aaaaatcgac tttgaaacga ctctcgacaa tcagatgaaa
     3301 gatcatcgct tacgtgtctt gtttcctaca aagttacatg tggaacatca cgaagcagat
     3361 agtatttttg aagtggtgaa gcgcccaaac catgtgtcta aaagttggga aaaccccacg
     3421 aatccgcaac atcagcaagc atttgtcaat attcatgatg aagagtacgg tgtaacagtc
     3481 ggaaactttg gtttgaatga atatgaagta acagaagacg gacaaatcgc agtgacgcta
     3541 cttcgcagtg tgggagaact aggcgattgg ggatattttc caacaccaga agcacagtgt
     3601 ctaggtgagc atcggttcaa ttacagcatt gaactacatg gaccagaaga aaaattctct
     3661 acttatttgc atgcatacgc agcacagatt cctttttcca ctcaacaaat caaacatcat
     3721 gaaggaacat tgatttcaaa acaacaatat ttaacgataa aaagtgaaac gtttgcaatt
     3781 acagctttga aacgcagcaa gtttagtgat aaagtagtag taagaggttt caatatgtct
     3841 agtcatttag aaaaattgga aataactaaa gacaatggaa aaactgtcat attgaatctg
     3901 cttgaagaac caacgaagca agcggttgtc ccaataatcc aaccttatga gattcggaca
     3961 atcggattcg aggaggaaaa cgaatgtatg gcggaataga agcaggcgga acaaaatttg
     4021 tgtgtgcggt ttcagatggt ggagaaattt tagaaaaatt aagtatccct accacaacac
     4081 cagaagaaac attggatcag gtatttgaat tttttgatcg ttatgaattg aagggaatcg
     4141 ggattggctc attcggcccg attggtatcg acaaaacaaa agataattac ggatacgttt
     4201 tggcaactcc caaaaaaggg tgggcacaat tcgattttct tggcacaatc aaggaacgct
     4261 acaatattcc tgctgcttgg acaacggatg tcaatgctgc tgcatatggg gaattgaagc
     4321 aaggagcagc acaaggaaaa aatagttgta tttaccttac cgtgggaaca ggaatcggtg
     4381 caggtgtcgt gatcaatgaa gaaatttttt cagggatcgc acatcctgaa atgggacata
     4441 tttgggtgaa acgccatcca gatgaccatt atgaaggtac ttgtccttat cataaagatt
     4501 gtttagaagg attagcagca ggaccgtcta ttgaggcacg cacagggatc aaagggcaag
     4561 atctgccgca agatcatcct gtttgggcta ttcaggctta ttatattgct caagcactga
     4621 tcaactatac gttgacgtta gcacctgaaa agattatttt aggcggtggg gtaatgaatc
     4681 aggatcatct ccttcaaaaa atccgtcagc aatttgttga attgatgggt ggttatatgg
     4741 aaacacctca agtaagtgaa tatatcgttc gttggggact gccgaatgaa agtgggatta
     4801 tcggtagttt gctgttagca gaacacgaac atcaaaagta agcagacaaa ttacacaaaa
     4861 agaacagagt cttatcatta tctgctgata agac
//
LOCUS       DAXWLH010000173         4859 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXWLH010000173 DAXWLH010000000
VERSION     DAXWLH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.177"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            64..750
                     /locus_tag="ACGE36_002412"
     CDS             64..750
                     /locus_tag="ACGE36_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFF8384766.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            752..1696
                     /locus_tag="ACGE36_002413"
     CDS             752..1696
                     /locus_tag="ACGE36_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFF8384767.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1895..2539
                     /gene="pcp"
                     /locus_tag="ACGE36_002414"
     CDS             1895..2539
                     /gene="pcp"
                     /locus_tag="ACGE36_002414"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFF8384768.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2618..3895
                     /locus_tag="ACGE36_002415"
     CDS             2618..3895
                     /locus_tag="ACGE36_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384769.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4079..4828)
                     /gene="pnuC"
                     /locus_tag="ACGE36_002416"
     CDS             complement(4079..4828)
                     /gene="pnuC"
                     /locus_tag="ACGE36_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFF8384770.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1565 a          857 c         1048 g         1389 t
ORIGIN      
        1 tatatttttt atgtgttttg ctttataatt aaaaaaatat tgtcatttta tggaggaaaa
       61 tatatggaaa attatcttag ccttttaggc gtcttgattg ttattctcgg gtttgcattt
      121 aaattagact cgatattgat cgtaatggtt gctttagttg ttacagcttt gactggcggt
      181 cttggcattc aaggaatgtt agaactttta ggtacaagtt ttgtaaacaa tcgcgggatg
      241 gcgattttta tcatcattat gttggcaaca ggaacgcttg aaagaaatgg tctgaaagaa
      301 tcagcggctt ccttgatcaa gcgattcaag aaagtatctg ctggtatgat tgtagatatt
      361 tatgggatct ttcgaatgat ctttgcggct tttaacgtga gttttggtgg agttgctgga
      421 tttgttcgtc caatcatttt accgatggca ttggggacca tcgaatcaaa agggttagaa
      481 gtccatccta agcatgaaga agaactaaaa ggtatggctt cagctatgga aaatgtctgc
      541 tggttctttg gccaagtact attcatcgga ggagcagggg cgttgctagt ccaatcaaca
      601 ctgaaagatc taggatatga cgtaacattg ggacagcttg ctttagtgga agttccagtg
      661 gctatcacgg ctttagttgt ggcaagtgtc tacttctatc ttaaagaaaa gaaattagcg
      721 aagaaatatt atccaggaaa ggggcaataa gatggacttt tttacaaatc ctgatatttt
      781 attaggcgat aaattactgg agatccttta tatcgtgatg ggactcgtat tgatttacac
      841 tggagtaaga aatttaacag ataaaaccaa tcctcatcgt tatgggtcag cctttttctg
      901 gacggtctta ggggtagtta ttgcaggagg aagatggctt ccggcaatcg tgaacggtgg
      961 cttgatcttt gcgatgacca taccagcaat tgctaagaaa gtcagcaaag gagaaagccg
     1021 gcttccatca aaagaatata tggaaaaaat gtcagacaaa ctaggaatga aaatttttat
     1081 tccagctttg agtatcggtg tctttgcgat cttgtttgct ctatttacca atttaggtgc
     1141 tttagtcggc gtgggcgtcg gtgtttttgc agccatgcta atcttgatgt tcttttctcg
     1201 agataatcga ccaagtacat ttttagatga tgcagcagat atgctgggca cagtagggcc
     1261 gttaagcatg ttgccaatgc ttttagcttc tctaggtgcg gtatttacaa gcgctggagt
     1321 tgggacagtg atttccaatg cagtaggtac gatcgttcca gaaggaaatg tgaacatcgg
     1381 catcatcatt tacgctcttg gcatggtgat atttacggta atcatgggga atgcatttgc
     1441 ggccatcacc gtgatgacgg ttgggatcgg tgcaccattc gtatttagtc atggagcgaa
     1501 tccagcgtta ataggaatgg ttgcattgac ttgcggtttt tgtgggaccc tgctgacacc
     1561 gatggctgct aacttcaata tcgtgcctgt agcgatgctg gagatgaaag acaaatacgg
     1621 agtgatcaaa aaccagttgt ttattgcgtt atttatgcta gttttccaaa ttgcttatat
     1681 gattctattt aaataataga agagaggggt tgtgacagaa taattgtcgc aacctcttct
     1741 aacattgtag tggaacacta tttttttata agaatatatt aagtttgatc tgttatgtta
     1801 tttaaactat tgtttccttt atttacgctg ttcaaggaaa atggtatgat aaaagataac
     1861 catatgttaa atcaatcgga cggaggaaca acagatgaaa gtactagtaa caggttttga
     1921 tccatttggt ggggacaaag tgaatccagc ctatgaagca gtaaaaaaaa tgccagatga
     1981 aatcagcggt gctgaaatca tcaaagtgga agtaccgacc gtattcgaaa aaagcagtca
     2041 agtagtcaaa gaagccatcc aacagcacca accagatgtc gtgatctgtg tcggacaagc
     2101 aggtgggcgg tcagcagtct cgttcgaacg tgtcgcaatc aatctagcag aagcaagaat
     2161 ccctgataat gaaggaaatc agccattcga tacggcttta gaagaaaacg gtccagccgc
     2221 ttactttacc tcattgccaa tcaaagcaat gacaaaaaat gtacaagatc atggtctacc
     2281 agcgtacatc tcttatacag caggaacatt tgtctgcaat gatatcatgt atcgtttgct
     2341 tcatttgatc gaaacacaat tcccggcaat tcgaggtggg ttcatccacg tgccattctc
     2401 acccgatcaa gtgatcgatc gtccagtggg tacgccttct atgtctttag aagacattgc
     2461 ttcttctttg acttatgcag ttgaagcagc agtagaaaat aaagaagata tttccggaaa
     2521 tgccggaaca actcactaat ttagcaagaa acactggctg gttgctaacg ttatgttagt
     2581 agccagcttt ttgtaattag aaaggaaaga aacagccgtg gaagaatggc aatccgtctt
     2641 tgaagaatgg tttccaaaag aaatcagcaa aagctatccc atcaaaattt ctaaacaata
     2701 cacaagcagt caacgttggg agatctatgc aaaattaacg aaaaaacagc gggaactagt
     2761 cgataagcac cgccgatatc taatcagctc tcgttttatg gaagaacatt atttagctgc
     2821 aacagattgg gtgttttctg acttcaagat caatccgttt tttcgaacaa aacgaagcca
     2881 gcaaaaattg tattgtgagt gtggtagaga attaaaagtg caatacatcg ttaaatcgcc
     2941 aaaaaccgga aagatcctaa aactaggcat caaccacttt gccgatcacc tgcatgtctc
     3001 tccgacagtg gcagcttcga tccatcaggg aatgacaaaa gttgatctgg ccttagacga
     3061 attattatgg cttaagcaga agaatatcga ttttcctgaa ggactatggc aaaaatactg
     3121 ttttgtcttg taccaaaatc gtcggatgaa acagccatat ttaccggata taaaattagc
     3181 tcagcggctc gcagaatttc gccaagtaga aatgccgatt tacatagcgg attatcaagc
     3241 attggaaaat gaaatcaaga aaataagtga acacatcaat gggcagtcaa aaaagcgaca
     3301 gataaaaaaa gaactatttg acgactttgc agaagaatta gtaaaggatg tagaagaatt
     3361 cttgatcaac tatcgcgcat ttttacgtaa agattggcaa tccatcgtat atgaagaagt
     3421 gccagtacat cctaatgctt attttgaaac atttatttcc gtgttgagaa agacaaaacg
     3481 tcaacgaaca ccagaagtca ctgctcagat ggaatatttt gcgaaaaacc aacgattcat
     3541 ccaaccaaaa atctatctat ttatttggaa gcaatattgt cgctatggtt ttacagaagg
     3601 gttcttcgac agcattcctc ggatagtcag aaatggtttt ttaaaagtat tgcgtaaaga
     3661 acgagaagct atacaatctg ctgataagaa agaccgtaca gtctcaaaag aaaaatggca
     3721 actagtagtg aaagatatcc aatctggaaa tgtgcaagag acaatcgaca aatggaaagg
     3781 aaaacattat cgttttactg aagctcagaa acaagcgttg gaatattatc agaagttaga
     3841 agaatcactg cgtttcaatg atgaggcaag aaagtatctg aaggaattac tataaaaaat
     3901 agaggctggg acagaagcgt ttaactccaa gaaataagaa gaaattcacg aaaattgctt
     3961 ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgatt ccgccgttta
     4021 tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct cagttttttt
     4081 atgcgtgttg ttgtgcgttg tagtattctt cttccatttt ttgcatacgt tcacgttctt
     4141 tttggttata ccaaggtgaa acggtaaatg caaggacatc attcagcaag taaacagagc
     4201 tgttcacgaa catcgccaaa cttgcagatc catgcgtaaa tgaaatatac caaaggacca
     4261 tttgagcaag ccctgaagct aaccaccagt aatattggtt gttgtaacgc aagaaactga
     4321 tgataccagc agtcaagcta atagaaaaag caatggcatc tacccacggc cgaggatcat
     4381 ctgtataacg accaatcagg aatcctgaaa caagatagac aaggaaagta ctgatgattg
     4441 cgataaacca gctttttcca gtgaattttc ggatttttga agccatatta tgattccatt
     4501 ctttacttaa caatacgggg atatccaatg tgatcatata agcaatctgt tcaccgatac
     4561 ttaaatagtt ctttgctgaa aacccaacga taataaaaca aatcgcagac aaaataccta
     4621 aaataccgtt gactgatttt gccgcattga tggataaaat acacaataca ccaagcgtgg
     4681 taccgataaa tgtaataacg gacagccaag tgatgggact attgacaaga gtcatgacct
     4741 ggcagccaag actgaaaaag aaaagataat aattttgttg cggccagcct tttagctgat
     4801 gagctaaaaa ctgaaaatat gacttcatgt atgaggactc cttaagacac taaatgtag
//
LOCUS       DAXWLH010000174         4786 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXWLH010000174 DAXWLH010000000
VERSION     DAXWLH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.178"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..813
                     /locus_tag="ACGE36_002417"
     CDS             <1..813
                     /locus_tag="ACGE36_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFF8384771.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACGE36_002418"
     CDS             786..1229
                     /locus_tag="ACGE36_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384772.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2401..3369
                     /locus_tag="ACGE36_002419"
     CDS             2401..3369
                     /locus_tag="ACGE36_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8384773.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="ACGE36_002420"
     CDS             3379..3849
                     /locus_tag="ACGE36_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8384774.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3865..4035
                     /locus_tag="ACGE36_002421"
     CDS             3865..4035
                     /locus_tag="ACGE36_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384775.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            4445..>4786
                     /locus_tag="ACGE36_002422"
     CDS             4445..>4786
                     /locus_tag="ACGE36_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFF8384776.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         1824 a          772 c          792 g         1398 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agctttagaa agttgctttt agctagacca attaaaaatc
     4261 acaaagtgag cgtctctatc cattggtaga ggggctactt ggcatagtgt acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DAXWLH010000175         4736 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXWLH010000175 DAXWLH010000000
VERSION     DAXWLH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.179"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(124..486)
                     /locus_tag="ACGE36_002423"
     CDS             complement(124..486)
                     /locus_tag="ACGE36_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384777.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(560..940)
                     /locus_tag="ACGE36_002424"
     CDS             complement(560..940)
                     /locus_tag="ACGE36_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFF8384778.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1022..2197
                     /locus_tag="ACGE36_002425"
     CDS             1022..2197
                     /locus_tag="ACGE36_002425"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFF8384779.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2274..2858)
                     /locus_tag="ACGE36_002426"
     CDS             complement(2274..2858)
                     /locus_tag="ACGE36_002426"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8384780.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3059..4057
                     /locus_tag="ACGE36_002427"
     CDS             3059..4057
                     /locus_tag="ACGE36_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8384781.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4169..4678
                     /locus_tag="ACGE36_002428"
     CDS             4169..4678
                     /locus_tag="ACGE36_002428"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFF8384782.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1501 a          890 c          837 g         1508 t
ORIGIN      
        1 tttgaaaata aaatgcacta aaaaactatc tttagcagaa catttcattg tattttttgg
       61 accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt
      121 attttagaag aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc
      181 tggttcttcc caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc
      241 aaagacaaac aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact
      301 aatcttgcta acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc
      361 agtctttcct tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac
      421 caaaaatttt ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct
      481 tgccaaaatt tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt
      541 ttcagaaaaa atacactttt tatttcttat tttctttccg ttctagttca gataatgctt
      601 cttctatcca aataggtaac tgctccgcaa tagtggtaaa tccgatctta cgatttttat
      661 tcgtgaaata cctttttctg cgatatggaa cagctggatt tgttttttct aacgttctta
      721 aagataaact gatttttttg gaatattcat caatgtcgat gatctgaact gttactggct
      781 gtccgacttt taacaatgaa tggatatttt tcgtataacc ggattgtact tctgaaacat
      841 gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc tgtatccctg
      901 tcacttttcc ttcaattatt tgtccaattt tataagtcat ctacttccct ctcttcgcat
      961 ttatcattat actgtaaata gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa
     1021 gatgatcgaa tttgataaac agatttcaag aaaagataca gaaagtgtaa aatgggacgc
     1081 aattgctgag acatatcaaa tggatgactt acttcctttg tgggtagcag atatggactt
     1141 cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg
     1201 ttatgccact ctgtctgata aactatatca ggctatcatt cattgggaac gccaacacca
     1261 tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc
     1321 tgcagcagta caaactttta caaaaccagg cgattctatt ttgattcatg acccagttta
     1381 tcctccattt tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct
     1441 agaaaaaaat aatcatttca tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa
     1501 caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa
     1561 agaagaattg aaacagttaa gcgaattgtg tttaaaacat caagtgattc tgtttagtga
     1621 cgagatccat caagatttaa ctttattcga tcatacattt acttccatgc taacgattga
     1681 tcctaattta gatgatctac tagttgcttt tacttccgca accaagactt tcaatctagc
     1741 tgcaatcaaa aattcaatgg tatttatcaa aaatccagag atgaaagctg attttgaaaa
     1801 acatttagtg atgaaccaac agcacgaaat caacactttt ggactgattg gaacccaagc
     1861 ggcttatgag acgggtggag aatggttaag ccaactgatt ccttatctag aaaaaaatgt
     1921 tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa gtgagaataa tgaaccctga
     1981 aggaacttac ttaatgtggt tagattttac cgcctatatt tcagacgacc aagaattgga
     2041 gaacactctt gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc
     2101 cggtcatggg catatgcgct tgaatattgc ttgtccagaa gaaactttac tggaaggact
     2161 taatcgaatc aagtatgctt tagagacaat cgattaaatc atttattcaa aataaaagga
     2221 cgagacctta ctaggtttcg tcctttttca taattactcc gtaccatacc aaactattct
     2281 gaaatttcaa ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg accacgttgt
     2341 acatttgcga tttcatctac tgtgtccatc ccttctagta catgtccaaa gactgtatgt
     2401 cggaaatcta gccaaggagt tccgccacct ttatacgctt caatgatttc tgatgggaat
     2461 ccagcacctt ctagttgtcc aagcatatta gctggcacat ttgtgttatt tacaataaag
     2521 aattggcttc cgtttgtgtt tggacctgaa tttgccatag ataaagctcc gcgaaggttg
     2581 aacagttcac gactgaattc atcttcaaaa ctttcgccat aaatactttc gccacccatt
     2641 cctgttccag ttggatcgcc gccttggatc atgaaatcag gaataacgcg gtgaaaaatc
     2701 acgccatcat aataaccttt tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct
     2761 agttcaggga ataattgaac agtaatgtca ccacggtttg ttttgataac agcttttggt
     2821 cctttttcac tttttaaatc tagttgtggg aatgccatat tgtttcctcc taatatttgc
     2881 atacttcata ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt
     2941 cttttatttt tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac
     3001 atttttgaga agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat
     3061 ggaaatctat gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta
     3121 cgcaggaatg cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca
     3181 attagcaact ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa
     3241 agcaagtgaa ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca
     3301 tttaaaagaa gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa
     3361 taaaggcatc cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc
     3421 aagaaaattg aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt
     3481 gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc
     3541 tatcgattgg acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg
     3601 cccggaattt cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt
     3661 gcttactccc tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg
     3721 gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa
     3781 ttatggattc tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa
     3841 tgccattact gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga
     3901 catctgtacc tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac
     3961 tgcagtgaac aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag
     4021 cacaagctta tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc
     4081 aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag
     4141 aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa
     4201 ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat
     4261 gtttttacaa aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa
     4321 cagcgtgcta acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat
     4381 attagcagaa aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg
     4441 cctttacggt atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg
     4501 ctttacgaat tatggttata tcgacaaggt gaaacctggt attttaaaag aactaaacag
     4561 ccacgatggc gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc
     4621 tgctgccagt cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt
     4681 caacaaaaaa agaaagaaaa cgtctattta gacgagttct ttcttttttg tttgtt
//
LOCUS       DAXWLH010000176         4662 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXWLH010000176 DAXWLH010000000
VERSION     DAXWLH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.180"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            33..524
                     /locus_tag="ACGE36_002429"
     CDS             33..524
                     /locus_tag="ACGE36_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFF8384783.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(548..2923)
                     /locus_tag="ACGE36_002430"
     CDS             complement(548..2923)
                     /locus_tag="ACGE36_002430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFF8384784.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            3249..3875
                     /locus_tag="ACGE36_002431"
     CDS             3249..3875
                     /locus_tag="ACGE36_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8384785.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            3898..4602
                     /locus_tag="ACGE36_002432"
     CDS             3898..4602
                     /locus_tag="ACGE36_002432"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8384786.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
BASE COUNT         1423 a          911 c          863 g         1465 t
ORIGIN      
        1 taaatattta tatgtatatc aggagggttc ctatggcaaa agatgctagc ttcgacattg
       61 tatcagaagt caatacagaa gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt
      121 ccaatcgctt tgacttcaag gattctacag tagaaatcaa attagagaac agcaatctag
      181 ttgtattgag cgatgatgat ttcaaaatcg aacagatcaa agatgtatta tttggtaagc
      241 tgatcaaaag aaatgtgcca atcaaaaata tccatttttc cgaatcagaa cacgctctag
      301 gtggcaaagc tcgacaaact gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga
      361 aaatcactac agctatcaaa aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc
      421 agattagagt gacaggtaag aaccgtgatg atctacaagc tgtgattgct ttattacgta
      481 aacttgactt accaatcgag ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg
      541 acgtctgtca ttcctcgttt ttttgattta ctgcttcttt tgataaccaa aaacgctgta
      601 ttccttcttc caattcccca acacttcgta cttctcttgc tgtttgccaa tgttgtggga
      661 ataaacgtcg ataagaaaaa ctagaaaatc gttgatctgc caatacaacg attccttgat
      721 ccgccatgcc acgaatcact cgaccaccag cttgcaagac tttgttcatt ccaggaagct
      781 gataagcgta agcaaatcca aggtgttcct tctcatcata atatgatttg atcagttcct
      841 gctcatgatt catctgcggc aaacctacac ctacgatcat actgccgatc aatcggtccc
      901 ctctcaaatc gattccttct gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt
      961 cagcgggatt tgtttgaaat tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg
     1021 tttcttggat tagcacattg ctgtcgggat attgctgtct gaatgcttcc accgcaagat
     1081 ctaaatattg ataagaagga aagaaaacga aataattccc gacctttcca tcagcaaaag
     1141 catgaatcgt ctctactaat tttgccagac tgtgttccct ttttcgataa gtcgtctcta
     1201 ggtaatttgc gatgaaaact tgctggtttc cttctgggaa aggactcggc agtttgtaag
     1261 ccagactttt ttgtcccccc agtgtttcct gataataaga gagtggtgaa aaactagcag
     1321 aaaaaagaat actgcttttt cctttatcca acgactgctc aagaaacaag cttggatcaa
     1381 tacaaaactc ttttactatc agatcgcgat acgttttttc gactgttgtt tcgtagtgat
     1441 catcataaaa ttcactgacc ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt
     1501 gttcttgtga tgggtgctca ggaaattctg ccagccattc tttaattttt tcgcttaatt
     1561 gataacctgc ctttactaaa gattcagccg gtgctttttg atgatgataa tcccaatgat
     1621 cttcttttgc tatttctcgg atcgaatcaa attcttttaa tagtttgttg aaacggcgat
     1681 gaagtttttt gaattccttt ggtatagctt ccttacttcg tttcgttttt tcataagata
     1741 gttctgccga atacatctct cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa
     1801 acagatagtc ctcgtttttt tcatcttcaa aaaaacgtct taaataaaca gttggatcaa
     1861 ataaataatt gtaatcccca atgattacgt cacaccagat actgacatct aatgatagtt
     1921 cgaaaggaca aagtgtatgc tttcttgcat aagtttcgat gacttcacga gtgatctgat
     1981 tctcatgatt caaaagatcc cataaacctt cattaatccg attgtaataa ccattggcat
     2041 atgggcactg atctggatta caggtcgtct catctaaaaa gcagatcttg tctttcgccg
     2101 tcaaagtgac actttttact tcagctcctg tatcttttaa tgctgtcata gcatcttcag
     2161 ctacttgtct agttattgtt ttagccgtta gataaaagat cttttctccc tcttcttccc
     2221 ctacagcttt caaagcgggg aataacgtag agattgtttt tcctgttccg gtaggtgctt
     2281 ctacaaacaa tttctgtttc gtacggatcg ttttataagc agctaccgct agttcacgct
     2341 gacctttcct atactcatca tgggggaatt tcaaagccat caaagaggca ttgcgtacag
     2401 tgcgccagtt ttcttgaaaa acaagccatt ctttataatc ttcagtcagc tttttaaaaa
     2461 aagtttctaa ctgctctctt gtctgatgtt caacctttct cgtgatcact tcttctgccg
     2521 tctggaagta agtcaattgc agatttattt cttgtaaatc atgttcttga ctatagatat
     2581 aggcatacac ccttgcttga tgaaagaaaa gttccacttg ttctggttct aaatcttcaa
     2641 atcgtggttc ggaggttttg atttcatcta tataataaat accgtctctt ttgaaaatac
     2701 cgtctgcacg gccttcaacg atcaggtaat attcatctaa ttgaactgtt gtttttaaaa
     2761 agacttcttt ttcatagttt tctcccgcat ctttttggag cttgcggtgg attctcgctc
     2821 cttctaatgc agtatggtta ctttttttac gattgtcgat acttccacgt cgcaaaataa
     2881 attctactaa gtgccgcaca gatatttttg actgtttgat catttccacc acctctttct
     2941 ctctaagatt acctgaaaaa acctagttta gcaaaaagag ctctctttcc aaacataaaa
     3001 aaacaccctg ctgattaggc agggatatag gatatagaat ttggattttt ggatgaatct
     3061 aatgtaacaa ggacatatta aaatgctatg aaaacttact ttccttctct ttagaatatc
     3121 caaatacttt tcatatagtc tgataattca gataagtaaa gagaaataag tacctgtttt
     3181 tgtttcctag caatgaatgc gttatactaa tggatgaaag aagttttggc tatcaaggag
     3241 gaatgcacat gcatatcgaa agattaaaaa caggaatgat tgaagaaaac tgttatttgg
     3301 tctacaacga agaagcactt ttgatcattg accctggaga agatgcggaa cgaatcaaac
     3361 aagaaatcaa aaaaacaaac caacagccgg tagcgatttt gcttacccat actcactacg
     3421 atcatatcgg tgcagtggaa cctttacgcg cgtattatca aatcccagtt tatgtcagcc
     3481 cactagagca aaaatggcta ggtgatccga ttttgaatct ttctggactc ggtagacatg
     3541 atgatattgc tgatgttatt gtctcgcctg cagaatatga atttgaaatg aaaacttata
     3601 ctttaggaaa catgtctttt cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca
     3661 gctttatttt tgatgatttt gtcgtttctg gcgacgccct gtttagagga agtatcggta
     3721 gaaccgatct gtatacaggg aatttagaac aattacttca tagtattcgg acccagctgt
     3781 ttgtattacc tgatgaattt gcagtctatc caggacatgg cgatgcaacg acgattgaac
     3841 aggaaaaaag gacaaatcct tttttcaatc gttaaaacga cttaggagga gaataaaatg
     3901 acagaaacta cactttatat cgtacgacac ggaaaaacga tgttcaatac aattgaacga
     3961 gtacaaggct ggtgtgatac acccctcaca aagcaaggac aagaaggcat tcattattta
     4021 ggtcgcgggt taaaaaatgt cgacttttca tttgcttatt ctagtgacag tggtcgtgca
     4081 atcgaaactg cccgtattat tctaggagaa cattctaagg gaaaagaaat tccttatttt
     4141 attgaaaaaa ggattagaga atggtgtttc gggtctcttg aaggtggata tgacatggaa
     4201 atgtggggtg tgatccctcg tgtattgaat tttaaaacat atgatgaaat gtttaccaca
     4261 gaagtttctt ttgaacaaat tgctaatgct gtatacaact tagataccgc tgggtgggcg
     4321 gaaacttatg aaacattgcg agaccgtgta tggacaggat ttgaagatat cgcccatcac
     4381 tgcgaaaaaa atggtggtgg aaatgtttta gtcgtatctc atggtttgac gatcgccttt
     4441 ttgcttaatc tgatcgatcc taaacagcct gttcgtgctg ggcttttaaa tggcagcgta
     4501 acaaaggtca cttatgcaaa cggtcgattt tctatccacg gcatcaatga tacgtcttat
     4561 atcgataacg ggaaaacaca tagtcgcgac tcgtttctat aatggggata ttttaatttt
     4621 taccagagaa tcataaaagg caaagtctga acgagggctt tg
//
LOCUS       DAXWLH010000177         4658 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXWLH010000177 DAXWLH010000000
VERSION     DAXWLH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.181"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(117..204)
                     /locus_tag="ACGE36_002433"
     tRNA            complement(117..204)
                     /locus_tag="ACGE36_002433"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Ser,seq:cga)
     gene            complement(349..915)
                     /locus_tag="ACGE36_002434"
     CDS             complement(349..915)
                     /locus_tag="ACGE36_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8384787.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1170..2501
                     /locus_tag="ACGE36_002435"
     CDS             1170..2501
                     /locus_tag="ACGE36_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFF8384788.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2467..2817
                     /locus_tag="ACGE36_002436"
     CDS             2467..2817
                     /locus_tag="ACGE36_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384789.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2909..3328)
                     /locus_tag="ACGE36_002437"
     CDS             complement(2909..3328)
                     /locus_tag="ACGE36_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8384790.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(3329..3673)
                     /locus_tag="ACGE36_002438"
     CDS             complement(3329..3673)
                     /locus_tag="ACGE36_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFF8384791.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4023..4619
                     /locus_tag="ACGE36_002439"
     CDS             4023..4619
                     /locus_tag="ACGE36_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8384792.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1258 a          864 c          811 g         1725 t
ORIGIN      
        1 ttgcatctgc ggtaataagc ccaaccaaac gaaaaaatag gaaaagctat ttttgtttgg
       61 ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga
      121 aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt
      181 gcattaccac tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc
      241 aagcctattt ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga
      301 cagtttgcag ccaacggact taccgatcaa attcatactg atagaacact atctgattat
      361 ttcaggatta tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg
      421 ttcttctgtt ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac
      481 ccatgtacca gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc
      541 ttctttaact atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc
      601 atgttcatca atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata
      661 gttcaaaccg agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt
      721 tagttgttca aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc
      781 cagataaatc ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc
      841 tttaaaccct tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata
      901 gacttttctt ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt
      961 accctatttt tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt
     1021 tcagtcttct ttaacttttt tttgcaaaat taaaaaaatt tggttaaatt caatgggttc
     1081 ccttacattt ttagttttga cctaaatgat agttgtccaa atctttcatt tcaagttata
     1141 gttaaagaaa atgaaaaaga aggtgtgtaa tggaaaacga aactgacgtt atttatattc
     1201 atcctcaaaa acgaatcgtt tcacagaaaa gaaagtattt ttatcttggt tttacaggtg
     1261 ttttttttct ttttattggc ttgctttcta atactcctac tgataattgg agtggattgc
     1321 taactattct tacctcgcca agcaatcttt tgacagatta ttttgcttta ggtgttttcg
     1381 gaagcgcatt cataaatgta gggattttga ccttactttc tgtgctttta gcttatcggc
     1441 acaaggtgat tttaaacggt cccttatttg cttcgattct aacagttacc ggtttctctt
     1501 tttttggcaa aaacctttat aattctatct caatcatttt aggtgtgtat ctatacgctg
     1561 tatttgtcaa taaacctttt tctcagtaca tcatgatcgg actgtttggt agcgtattat
     1621 caccggtcgt tagttatatc acttttggta tgcgttttcc tttactgata ggtattttat
     1681 tggggaattt agctggcata gcaattggta tacttcttcc gcccttagct gctcaaactt
     1741 tagttttcca tcgtggattc acactttata atattggatt cacctctggg ttgattgcga
     1801 tgacttttac tgctgttttg aggttattca gctattcaat cgtcgaaaat acattggttt
     1861 tcaatgaata tcactttcct ctcatttgga ttatttttgg ctttttttcg ctgacagtcg
     1921 gaattggttt ctattataat tcctttcggc tttcggggat tcgagaaatt ttcgattcat
     1981 ctggaaaatt gacaactgat ttcattgcta attctggtat tggtgctact ttgatcaaca
     2041 tgggcttagt tggtctgatg ctgtcttctt atgttttgct ggttggcggt cagttaaatg
     2101 gacctgtcat cggtgctatt ttatctgcgg tgggtttcag tgcttttggt tgtcatttaa
     2161 aaaacagttt tccgatacta gtcggaatat ttattgcttc tttgtttgga acttttcatg
     2221 agatcaccag tactggcatg cttgttgcag ctgtgtttgg gacaggtctt gcgcccataa
     2281 gcggttttta cggcagtttt tacggtgtaa tagctggtgt gctacatatt gctcttgtcc
     2341 ataacgtcag cactttgcac ggaggactaa atctatataa cagtggtttt tcaactggtt
     2401 tcgttgcagg tatccttgta ccgatactag ataactttac cgctgtcaga aaggaaaaaa
     2461 agacacttgg aaaaagaatt atcaaaaaga atcatcgatg agcttcttac ttatttatat
     2521 tcccatgaaa taactgaaat acgtattgga gtagaatttg ctgaggaagg gcttttcata
     2581 gagatacagg gtacggcaga aactcagcca gaagatttag atgtacttgc tgatttgctg
     2641 aacaatcctc gggaccctag tatggaaatc tactatcaag aactattagg taatccccga
     2701 catgatcggg aagattatca tctgttaggt agtttgattg atgacgtgga aatcatgtat
     2761 gcccagccga ttatcaatat taagatcttt cgacgtgctt ctcattccta ttattaaccc
     2821 tattttctat ttataaaaga ctgaatcaaa aaagcagctc ctgaaagttc gattaaaatc
     2881 tactttcagg agctgttcaa gcttgacatt attgattttc caatccttca tacacttcat
     2941 ctgttacagg ttctaaaaac tctgaattac cagcagtgat cgccaaatgt tcaaaccaac
     3001 tgtcttttgt cgcaccatgc caatgtttaa tccctttgtg cgttacgacg acatctccag
     3061 ccattaatcg tcttgctggc tttccttctt cctgatacca gccttctcct ccggtcacaa
     3121 gtaagatttg atagccgtct gtatggacat gccagtgatt gcgacaacct ggttcaaaag
     3181 ttacattacc tacaccaaca gaaacttcgg gatcattcac taacatattc agatagcttt
     3241 gcccggtaaa tacttgagcg taagcttcgt ttttttcacc tgctggaaaa atcacgccat
     3301 tgtgtatttc ttcgtgtttt gtcatcattt acgcctcttt cttatcttcg ctattttctt
     3361 ctaaatagat ttcttttgct aaactaaaag ctgaccaagc ttttggccat tctacataaa
     3421 aagctaaatg ggtaatgatt tctgctattt cttcactagt aatgccattt tgcttgccaa
     3481 tcttcagatg agctggcaat tgttcaaaat ttcctcctga aattaatgca gatatggtga
     3541 tgatactacg ctgatgagca gataattctt ctctggacca tacttgtcca aataaaacat
     3601 catcatttaa ttctgcaaat tttggtgcaa aatctcctaa ttgatctcgt cctgcggttt
     3661 gtttttctgc catttctctt attctccctt cacgactgtt aggtgattca actatggttc
     3721 taaggcaact ttattttcta ttgtttcttc gcatttcatt ttaaatgtag tctttcagcc
     3781 tgtccaatgc ttagtttatt cagtcgatat gctttttaag catagttgct aacgcttttt
     3841 ttattttaaa aaaaacaagt tattaaataa gtctttagtt ggatgttttt ttgagatcaa
     3901 tcttttatac ttatgatgaa cttacaaaat tgtttgtttt ctgtcatatc aaaaaatggc
     3961 gcagttagtt gcttttctag taaagtatag ttaacaaaga acctctaagg agttgatgaa
     4021 taatgaatat tgctgcctct cgaaaactga taaatttcat atccattatc ggattaggcc
     4081 tatcaatcgc gctgacgatt tattttatta atttaggtgt tttcaaagat ttgaatgcgt
     4141 taagaggatt agtaggtgat tccatcattt taggaccgat tatttttgtc ttcatccaaa
     4201 tcctgcaagt agttattcca atcattcctg gagggatcag tactgcagct ggtgtcctga
     4261 tatttggccc ttatgctgga tttatctata actatattgg aatctgcatt ggttcgatta
     4321 tcatttttct gctagggcgt cgttatggaa agccattcat actttccatg attagcgata
     4381 agacatataa caaatatatt ggctggctgg ataatcaaaa ccgttttgag aagctgtttg
     4441 ctctagctat ttttcttcca gttgctcctg acgatgcgtt atgtttgatg gctggattga
     4501 caaacatgtc agtgaaacga tatacactaa tcattttgat tgctaaacca ctgtcgattt
     4561 tcttatatag tatggccttg atttatggag gtcaatatct aagtggtctt cttggataat
     4621 atttattttg taaaattagt cattgattga caatgtta
//
LOCUS       DAXWLH010000178         4600 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXWLH010000178 DAXWLH010000000
VERSION     DAXWLH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.182"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(709..2214)
                     /locus_tag="ACGE36_002440"
     CDS             complement(709..2214)
                     /locus_tag="ACGE36_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8384793.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2211..2897)
                     /locus_tag="ACGE36_002441"
     CDS             complement(2211..2897)
                     /locus_tag="ACGE36_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8384794.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3090..4511)
                     /gene="gndA"
                     /locus_tag="ACGE36_002442"
     CDS             complement(3090..4511)
                     /gene="gndA"
                     /locus_tag="ACGE36_002442"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFF8384795.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1284 a          948 c          758 g         1610 t
ORIGIN      
        1 gccaggatca aactctcata aaaagttcga acaatcttgc gattgttaag ctcaatttgt
       61 ttgctagcat attgcttgct tgttaaaaat gtttgttgtc ttgaattgaa tcaagacgcc
      121 ctacacattt ggtttgtctt actttgttca gttttcaaag gtctaatctt tttgttttaa
      181 caactcttta atcttatcag attcagaaga gaatgtcaac aacttctttg tttatttttt
      241 ctaaacgtct ttgccactca ttagcagcga cttgattatc ataccatctt tattgttatt
      301 tgtcaataac tttttcgaag tttttttcga taaaatcaag ctttattagc tatctcatcc
      361 aaacgacttc gttattataa catgacatca aaagtgcgtc aacactattt tttataattt
      421 ttagtgacac cttaaaggaa tcatttagaa caaataataa tatagcaatc tctttataaa
      481 attgcaatag aaaattccct atttttattt attattttta aacacgaata ttacagcatt
      541 ttattgtttt ttcgttcatc taaaagatac ttacatttaa ttaacggatg ttattctatt
      601 taattgttcg ctagtttttt tataatgaca tccctgaatt tctcacttgc ttgtattttg
      661 taccttattc tttgttggat atataaaaaa acaagtaaag cacatgcttt acttgtcttc
      721 tgcctttgcg ataggaataa agattctaaa aattgtccct tgccctacta cactttctgc
      781 taagatacgt cctttatagt tttcaaccag ttgtttagcg atagacaacc ccagaccatt
      841 tccacccttc gttctagctc ttgctttatc cactcgataa aaacgattga agatcttatt
      901 aaggtcttct ttgggtattc cttcgccaaa gtcctgaatc gcgatctcaa attcattcag
      961 agtggaagaa atagagatat ggacttcttt tcgtgtagtt gaatacttta ctgcgttatc
     1021 caatatgata atgattaatt gttcaaagtg attacgataa atttgcaggg tcacttcttt
     1081 tgtcaaatca tcatctaaag tgatcacata gtctggatat aatatcttga aattattata
     1141 aacttgatag gtgacttctt ttcctttagt tgtttcattc gcataatgaa catctacttg
     1201 ttctgctctt gataagtcta gcatctcttg aactaagctt ttcatacggc tgatttcttg
     1261 catgctggcg cttaatgatt cttctaatat ctcaggatca tctttccccc aacgatttag
     1321 cagactaaga tgcccttcaa taattgcaac aggtgtcctt aactcatgag atacgtcttc
     1381 cacgaattgc tcttgttgtt caatatacaa acgcatacgg tcaagcatct cattgaatat
     1441 ttcagctaaa tctgccaatt catcatttgt gtcgatttca ggcatgtgga tatctgattg
     1501 cggatcttta cgtatagtat ccattgtatc tctgagtacc ttgagtggtt ttaagaaata
     1561 agaagataaa aagaaaccta aaatactact taagatcaaa gaaacaactt ctaaaacaat
     1621 aagagtcaat aatagcttat ttcgaatatc atagaaagaa gataattcat agaagctttg
     1681 cgcatagccg atcttttctc ttgtaccttt agaatatata ggctggatag ataaaaagcc
     1741 agtttttcct tctagtgtaa cgatcgttgg aactttacgt gtagtctgaa tcaggtttcg
     1801 actgttttca tgggttttaa aaatcaagtt ctcgtccaaa tcataaacat aaagatccat
     1861 tgatggctgc cctagctcag ctataaagga atcgatcttc attaagtttc cttcgaccag
     1921 tgaagtatga tctacgacct catctccgtc atttgtcgct ttcgtcagat tacgatacgt
     1981 atttactaat gtcagttctt catccgagtt agccaaacga gaagctactt ctgaaatcgt
     2041 acgctctaca ttgtttcttt cttttgccac aatcagattg atagatgatt tataggtaat
     2101 gacagcgaaa attgtaaaca caacgaatat gaagaaagaa ctcgcaaagg cccacttgat
     2161 tgtcaaggag ggccctttca gttctctttt ttccgatttt ttcggcttta tcacgagcgc
     2221 atcacataac ctgttccgcg tacagtttgg atatagcttt cctccccggg aacgtcaatt
     2281 ttgttgcgaa gataacgaat atatacgtcc actacgtttg tttcaacttc tgtttcgtat
     2341 ccccacactt tatttagtaa aacgtcacgt gctaatacca cgttcacgtt ttccattaaa
     2401 gtaagcagca actcgtattc tctttttgtt agttcaatta cttcatttcc gcgacgtacg
     2461 acacggtttt ctttttcgat agtcaaatcg cgataagtaa tagtagtttg tttagccaca
     2521 tttttatcgc cttcaatatc gattcgacgc aacaatgcac gaagacgtgc taataattct
     2581 tcgatagcaa atggtttgac gatgtagtca tctgctccgt ggtcaagtcc agatacacga
     2641 tcgatcacag aatctcttgc agtcatcata atgattggtg tatttttgac ttggcgtaca
     2701 cgtctgcaga cttctaaacc gttcaattct gggagcatca gatccaacaa tatggcatcc
     2761 cactcattat ttaatgccgc atctaaacca gttcggccgt tgtagtggac ttctgcgtta
     2821 tacccttcat gcttcaactc tagctcgaca aatcgtgcca agttcttttc atcttcaatg
     2881 attagaatat tgctcattta ttattttatc ctttctattc tactggcttt ttaaaaactc
     2941 tatcattata ccgataatat gcttaaaaat ctatagtcat tttatcatgg atagcaattt
     3001 taaaaaagag ctgaagtcta ctcataacaa tcgacttcag ctctttcgtc ctctttttat
     3061 tttctcattt cctaacagcc gaaagactat tattcttcat ggtaccatga gtaatggaag
     3121 attccttctt tgtcgacacg ctcataggta tgtgcgccaa agtaatcgcg ttgggcttgg
     3181 attatatttg caggcagacg ttctgctctg tatgaatcga agtaagcgat ggctgatgag
     3241 aaagtcggta caggtacgcc agcttggaca gccaaggcaa cgacgtctct gactgattgc
     3301 tgatattttt tcgtgatttc aacgaagtaa tcatctagca ataaattttc gatctcagga
     3361 tttttgtcat aggcatccgt aattttttgc aagaatcgag ctcggatgat acatccagcg
     3421 cgccagattt ttgcgatttc accaaatggc aagtcccaat caaattcttt tgaagcgaca
     3481 cgtaattgag caaatccttg ggcataactc atgattttac tgaaatacaa cgcttctcgg
     3541 attttttcga ccaattcttt tttgtctccc gtgaactcaa aatcgttcgt acgagacaaa
     3601 atcttgcttg cttgtacgcg ttcttcttta taagcagaaa tatagcgggc aaatacagat
     3661 tcagtgatca atggaagtgg cacacctaag tcaagcgcgc tttggcttgt ccatttacct
     3721 gttcctttat tcccagctgc atcaaggatc acatctacta ctggtttacc tgttccttca
     3781 tcgtctttac gtgtcagaat gtcagcagtg atttctatca aatagctgtc taattcgcct
     3841 tgattccatt ctttaaagat atcagccatt tcttctactg aaaggtttaa aatattcttc
     3901 attaagtcat aagattcagc aatcagttgc atatctccat actcaatacc gttgtgtacc
     3961 attttcacat aatgtccggc tccatttggg ccaatatatg tgacacatgg tgctccgtct
     4021 tctgcttttg cagaaatttg ctctaagatt ggtgcaacta gcgcataggc ttctttttgt
     4081 ccaccaggca tgattgaagg tccttttaaa gctccttctt caccaccgga aacgcctgta
     4141 ccgataaagt tgatgccaga gttggctaac tcttcattac gacgcatcgt atctttaaag
     4201 aatgtatttc ctccgtcaat caaaatatcg cctttatcaa ggtgaggaag taactcttgg
     4261 attgttgcat ctgttgccgg tcctgctttg accattagca agatccgacg tggtttctcg
     4321 atagattcta caaattcttc gattgtatat gttgctttga aatttttatc tgggtgttct
     4381 tctactacag cggttgtttt agcccctgta cgattgaata aagccactga atagccgcgg
     4441 ctttctatgt tcaaggctag atttttaccc ataacggcca taccgacaac gccgatttgt
     4501 tgttttgaca tatgaactcc tcctacatac gggatttctc cctaatctct tacattatag
     4561 cttaacacgg ctacattgca aactaaagaa caacatattt
//
LOCUS       DAXWLH010000179         4583 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXWLH010000179 DAXWLH010000000
VERSION     DAXWLH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.183"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(52..522)
                     /locus_tag="ACGE36_002443"
     CDS             complement(52..522)
                     /locus_tag="ACGE36_002443"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFF8384796.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACGE36_002444"
     CDS             complement(681..1139)
                     /locus_tag="ACGE36_002444"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFF8384797.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACGE36_002445"
     CDS             complement(1310..2455)
                     /locus_tag="ACGE36_002445"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFF8384798.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACGE36_002446"
     CDS             complement(2468..3385)
                     /locus_tag="ACGE36_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFF8384799.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACGE36_002447"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACGE36_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFF8384800.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1430 a          838 c          870 g         1445 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tca
//
LOCUS       DAXWLH010000180         4581 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXWLH010000180 DAXWLH010000000
VERSION     DAXWLH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.184"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..130
                     /locus_tag="ACGE36_002448"
     CDS             <1..130
                     /locus_tag="ACGE36_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8384801.1"
                     /translation="TASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGVTA"
     gene            181..372
                     /locus_tag="ACGE36_002449"
                     /pseudo
     CDS             181..372
                     /locus_tag="ACGE36_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262465.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            385..1371
                     /gene="lacD"
                     /locus_tag="ACGE36_002450"
     CDS             385..1371
                     /gene="lacD"
                     /locus_tag="ACGE36_002450"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8384802.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            1827..2495
                     /locus_tag="ACGE36_002451"
     CDS             1827..2495
                     /locus_tag="ACGE36_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8384803.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            2486..3619
                     /locus_tag="ACGE36_002452"
     CDS             2486..3619
                     /locus_tag="ACGE36_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8384804.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3914..4339)
                     /locus_tag="ACGE36_002453"
     CDS             complement(3914..4339)
                     /locus_tag="ACGE36_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8384805.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT         1458 a          783 c         1006 g         1334 t
ORIGIN      
        1 tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg
       61 aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat taatgggagt
      121 aacagcctaa gatatttgga gttcagagag ggcgcgtttg attttcaaac ttcgcaacag
      181 aacccagttg gctctggtga tgcgaccgtt gccggattga ccgctgcgat tcaacaaggt
      241 ctttcggatg aagccgtgtt aaaacagggt aatgttttag gaatgctgaa tgctcaggaa
      301 gcgacgactg gctatgtcca attagccaat tatcaaaaac tatatgatca aatcttagtg
      361 gagaaagtgt aggaggatac ggaaatgaac gaagaaaaac gtcaatatat caagcaacta
      421 tgcaatgaag atggagtgat tggggcctta gcaattgatc aacgcggggc tttgaagaag
      481 atgatcacaa aattcaaggg cagcgaagca gaagaatcag aaatcgttga atttaaagaa
      541 attgtttctt cacaattaac gaaatatgcc tcatcgattt tattagatcc cgaatatggc
      601 ttgccagcag ctgacgttag agcaaaaaat gccggcttgc tgttagctta tgaaaaaact
      661 ggttacgatg cgtcaactcc cggccgttta ccagatttat tatcgatctg gtcagttaaa
      721 cgtttaaaag aagcaggcgc cgatgcttgt aagttcctac tttactatga tgtcgatgaa
      781 gcagaagaaa tcaataatca aaaacatgcc tttattgaac gaatcggctc agaatgtcag
      841 gctgaaaact tacccttctt cttggagctt gtttcttatg acgcgaacat ctctgatgcg
      901 acttcgaaag aatacgccat taagaaacct cataaagtca acgatatgat gaaggaattt
      961 tctgatccac gttatggcgt agatgtgtta aaggtggaag tacccgtcaa tatgaattat
     1021 gtggaaggct atgggacaga cgaagtcgtg tacacaaagg cacaagcggc taacttcttc
     1081 aaggaacagg ctgaagccac taacctgcca tttattttcc taagcgccgg tgtaaaagca
     1141 gagttgttcc aagaaacact gcgcttcgcg aaaaatgccg gctcaacctt caatggtgta
     1201 ctttgcggcc gtgcgacttg ggcagatggt gtagaaagct atatcgttga tggaaaagaa
     1261 gcagcgatgg agtggatgaa tacgcaaggg cgtaaaaata ttgaagaatt aaatgccgta
     1321 ttgaaagaaa ctgctacacc ggtggaagtg ttaacaagct cactatcata agaaatcaga
     1381 ctgtgaagca aaattgcttg cagtcttttt ttatacataa agcgtaaaat catgctgcat
     1441 tattagggga acgaattcta aaatagtaag tgagttaaaa cgaaggggtt tttctttaga
     1501 gagattttct tacatgtggt acttaatgac ttgatggcaa ttttcagtat tctaacgagg
     1561 cttattggtc ataaaccaac gaactttgaa aagtatatac aagaaaacca agcaaataga
     1621 aagtagtgag actggaggaa aggttccagt ctttttctat tctaggtatt ttcaaacagt
     1681 aaaatcattc ttaattatgt gttttttaac agaatgtatg aatttcttca aatcttcaca
     1741 aaaagaacgt ggactttgaa gaagagcgtg tactatgcta aaaagcataa gtgtaattac
     1801 taaatccgta tgaggagtga atatacatgc gtgtactggt ggttgaggac aatcgtgagc
     1861 tggttttatc cgtaaaaaag ggcctagagc aggagaatat ttctgtagat gtagcttatg
     1921 atggcgagtc aggagaagag aaggccttca tcaattcata cgatgggata ctactagatt
     1981 taaatctgcc taaaaaagat ggattatctg ttttacaaag tctccgtgct gaagaagtcg
     2041 aaagtccagt tatcattatt actgcacgtg atgagattga agaacgagca aaaggcttgg
     2101 attttggagc agatgattat ttagtcaaac cctttgagtt actagaatta aaagcacgaa
     2161 tacgcgcggt tattcgtcgt tatcatggac gtgcgctgcc gcagatttca attggtgctt
     2221 tgaagattga tccattaggt cgaagggtca gctatcaaaa gacagaggtg gtcctgaaac
     2281 ccaaggaatt tgatatctta ctgtgcatcg ctcagcgtca tccagcggtt gtttcgtctg
     2341 aggaaatttc tgaacatgtc tacgatgaag attttgatcc tttttcgtcg gttctgcgag
     2401 tgcatctggc tcgtctaaaa aagaaactgg ctgaagcagc tgaaaaagag gtcttaaaaa
     2461 ccattcgagc aaaggggtac cagctatgct attaaatttt aaaatgacgt taacgatgct
     2521 ggctttttca ctgttggtga caggcgtgat tttagtgagt ttcttctttc tgagaaataa
     2581 tttggaggga atcagtatcg catctacctt aggtgaagat gtttatttta tggaagaggg
     2641 taagacggtt caagcagaga cgcagatgga agcttcgaaa tcttccaaca atcaatttat
     2701 gactatttcg atgatagata aagcttacca ggacgaacta tttaaatatt taccagcgat
     2761 ggtgctggtt atttgttctg ccgtcatgtt attaacgttt attctttggt tggttttacg
     2821 caaaatttac agtaatcaaa tcttagcgat tacgcgtgat ttaacgttta ttgatcaagt
     2881 tcctaaaaat actgtgaagg acgaggcatt aaatgcggct tttaataagc taaaggataa
     2941 gttcgatggt catttggagg attatcgacg attaaatagt tatctgaccc acgagcagaa
     3001 aaatgccatc gctattttgc gtacgaatct ggaaatagaa cggcaacatg ccgatcaagg
     3061 aaatttaatg cttttagatc ggatgaccga cagtattgac gatattttga cattatccaa
     3121 taactcagag gatcttagca cggaagtcgt agacgtttca ctgatctgtg cggaagtttg
     3181 tgatgcctac aaaatgaatt atccgaatct tttgtttgcg tttactgaag atcaaccaac
     3241 cctgattcat ggacgagatc gttggattta tcgggcggtt tcgaatttag ttgacaatgc
     3301 aatcaaatat ggtgaaggaa aactggtaat tgtcaccatt tcaaaccaaa agggtagcgt
     3361 gattattgaa gtaaaagatc agggaattgg cattcctcag gagaaacagg catcaatttt
     3421 ttctcatcag taccgagtgc atgggctcaa gcaggatggc ttacctaaaa caatggactt
     3481 tagggacacg gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag
     3541 aaaatcatta aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat
     3601 attgtctagc ttaatttgac aaatcagatg tctataaaat tcgttagaaa atacaattta
     3661 tagatatcca attgatttgt aaacaaaaag gagttgtcaa ctaagccact cctttttgtt
     3721 gtaatttttc ccaataagaa agtctactac tttaggaaga taatagtaag atagaaatgt
     3781 tgcccccaaa attattatcc agctggatat aattaatcaa ataaaaagga tcaaaaatgt
     3841 agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactctttt tttcatgctc
     3901 taaaatgata tttttacaca accgtaacgg ttgttttacg aaattggacg ggactttgcc
     3961 agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta tcgatggctg
     4021 attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt tttaataagc
     4081 caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac atgctttgaa
     4141 agattttgta ctccttcaat ttgtttctat aagctttcat ttgatacgac caaccttgat
     4201 ctgagtgaaa cgtacgacga tagggacaat cgttagtggt ctggatagct tcctccaaac
     4261 cttccattac cgcacgtgtt tctgaatggt ggtagcgacc gatttgaacg gctagggcgg
     4321 aaatacggtg caaatgcacg gtggtttcat gcaatgctta cagggcgtat tttgtaaggg
     4381 tatgtaacgg agataatccc gacggtaatg gatgtgagag gccaagccga cgctgacggt
     4441 agggcaaaca aacatagttg cactctagcg atcgttattt atttttaacg gtcaaactag
     4501 ggtacaacta tgcttaaaac acttcgcctc aacttctggt agcaaaagtg attttgaaag
     4561 tcaaaatatt ttttatgagt t
//
LOCUS       DAXWLH010000181         4575 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXWLH010000181 DAXWLH010000000
VERSION     DAXWLH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.185"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            22..783
                     /locus_tag="ACGE36_002454"
     CDS             22..783
                     /locus_tag="ACGE36_002454"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8384806.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(898..1338)
                     /gene="fabZ"
                     /locus_tag="ACGE36_002455"
     CDS             complement(898..1338)
                     /gene="fabZ"
                     /locus_tag="ACGE36_002455"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8384807.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            1558..2313
                     /gene="fabI"
                     /locus_tag="ACGE36_002456"
     CDS             1558..2313
                     /gene="fabI"
                     /locus_tag="ACGE36_002456"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFF8384808.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(2423..3475)
                     /locus_tag="ACGE36_002457"
     CDS             complement(2423..3475)
                     /locus_tag="ACGE36_002457"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFF8384809.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            3601..4521
                     /locus_tag="ACGE36_002458"
     CDS             3601..4521
                     /locus_tag="ACGE36_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFF8384810.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1402 a          900 c          859 g         1414 t
ORIGIN      
        1 gtaaattgga aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca
       61 gcagcattgc tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct
      121 acagaaagtt caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg
      181 aacgcattgg aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa
      241 atggtgacga ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg
      301 gttgaaaact ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc
      361 gtcatcaaag atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa
      421 agcatttggg gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt
      481 gcgttatcta tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt
      541 caaaatacag atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc
      601 gacgcttata aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa
      661 gtgattgaag gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac
      721 aaaccaaaag aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa
      781 taaaaaaata acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt
      841 ctttttacat aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca
      901 agcattttgg ttgacgatga acgtgaactc gcactcacag acttttttat catctacata
      961 tgctgcagat aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt
     1021 cagcacatct cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata
     1081 agctcttttt cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag
     1141 tgtttctaga atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa
     1201 atcttcattg attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc
     1261 tacataatca atataacaaa taggataacg attaggaatc aattccatta cttcagtagc
     1321 agataatacg taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt
     1381 tgactatcaa agtatcatat tcctaaatat ttagcaatct ttttttaatt taagaaaaaa
     1441 tagtttgaca atcaaaacat tttgactaat aattaagaaa tcaagaagtt attttagcta
     1501 ttattctcta tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg
     1561 tcatttctag aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct
     1621 tggggttgtg ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat
     1681 gaacgaatga aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt
     1741 gatgtttcta gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg
     1801 gagatccacg gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat
     1861 gtgtctgata tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta
     1921 attgctgtat caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg
     1981 acttatctag gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct
     2041 tctcttgaag ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc
     2101 aatggaatct cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa
     2161 aaactgatcc agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa
     2221 gtcggtaatg cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt
     2281 atctacgttg acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa
     2341 catataaaaa tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt
     2401 ttttcaactc attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta
     2461 tgtctccaaa ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt
     2521 ccgtttcact ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa
     2581 taacagccgc tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa
     2641 tatctccttc aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt
     2701 tcgtgatact tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa
     2761 ttccaaagaa atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat
     2821 aaacacaatc tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt
     2881 aatttccgcc gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata
     2941 caataaaatt cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga
     3001 cgacaaataa atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta
     3061 aagggcgcaa gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt
     3121 ttaccacttg cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc
     3181 gtctagcact ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc
     3241 cgtcttttcg acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg
     3301 gagagccttc atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc
     3361 cgtctttcca tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt
     3421 tccaatcgat ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc
     3481 atattatttg tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc
     3541 cttttttgaa aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt
     3601 atgttttcat tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat
     3661 caagctgtca gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgcc
     3721 attttggctg cctttatcca aaaaagttta tatctgattt ttttgatcgt ccttttttgg
     3781 attcttcacc gtgtgggaaa atatctgatt gataagtctt ttaatcagta ttctaaaaaa
     3841 gccattctaa acgagagccg cttaaagact ttacatagtc ttttaaatac tatttttcat
     3901 tatacccttg gtttcttttt tatctatgca attttatctg ctatcggtgt gccgatcgga
     3961 tcacttttgg ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc ccaagggttc
     4021 atgagtgatg ttattaccgg attttttatt atcatggaac aacagatgga tgttggggac
     4081 tacatcaagc tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg tatccgtacc
     4141 cttcagctga aagcaacgga tggtactgtc catttcatcc cgaatcgaaa catcaccacg
     4201 atcagcaatc tctctcgtgc aaatatgcaa gtacttttag atattcggat tgttcctgaa
     4261 gaaggatatg acaaaatcta tgagatcatt gatcgagtaa atcgtacatt agctgaaaaa
     4321 taccaagaag agctccaaac tgaaccttct atctttggat tagtggatct tggaaacagc
     4381 aattttgcga ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc cttaaaagaa
     4441 gagttcttga gctcctatgt caaagaattg acagcttctg gtttcacgat tccgaacagt
     4501 ccaattgctg tcggaaaata atttggacaa acgatgcaac gaatctcact aaaaagagcg
     4561 tgggacaaaa gtaaa
//
LOCUS       DAXWLH010000182         4567 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXWLH010000182 DAXWLH010000000
VERSION     DAXWLH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.186"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..301)
                     /locus_tag="ACGE36_002459"
     CDS             complement(<1..301)
                     /locus_tag="ACGE36_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFF8384811.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            394..743
                     /locus_tag="ACGE36_002460"
                     /pseudo
     CDS             394..743
                     /locus_tag="ACGE36_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1015..1305)
                     /locus_tag="ACGE36_002461"
     CDS             complement(1015..1305)
                     /locus_tag="ACGE36_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384812.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1707..1943
                     /locus_tag="ACGE36_002462"
     CDS             1707..1943
                     /locus_tag="ACGE36_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384813.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1950..3902
                     /locus_tag="ACGE36_002463"
     CDS             1950..3902
                     /locus_tag="ACGE36_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8384814.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="ACGE36_002464"
     CDS             complement(3974..4471)
                     /gene="dfrG"
                     /locus_tag="ACGE36_002464"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8384815.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1561 a          804 c          756 g         1446 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagattt aagtagttcg gttttggagt acaaaaagtg
     1501 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
     1561 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
     1621 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
     1681 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
     1741 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
     1801 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
     1861 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
     1921 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
     1981 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
     2041 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
     2101 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
     2161 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
     2221 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     2281 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     2341 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     2401 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     2461 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     2521 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     2581 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     2641 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     2701 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     2761 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     2821 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     2881 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     2941 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     3001 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     3061 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     3121 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     3181 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     3241 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     3301 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     3361 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     3421 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     3481 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     3541 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     3601 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     3661 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     3721 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     3781 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     3841 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     3901 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     3961 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     4021 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     4081 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     4141 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     4201 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     4261 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     4321 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     4381 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     4441 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     4501 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     4561 aagcaat
//
LOCUS       DAXWLH010000183         4537 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXWLH010000183 DAXWLH010000000
VERSION     DAXWLH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.188"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            14..673
                     /locus_tag="ACGE36_002465"
     CDS             14..673
                     /locus_tag="ACGE36_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8384816.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            758..1663
                     /locus_tag="ACGE36_002466"
     CDS             758..1663
                     /locus_tag="ACGE36_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8384817.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1665..2297)
                     /locus_tag="ACGE36_002467"
     CDS             complement(1665..2297)
                     /locus_tag="ACGE36_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8384818.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2448..2573
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2617..3141
                     /gene="infC"
                     /locus_tag="ACGE36_002468"
     CDS             2617..3141
                     /gene="infC"
                     /locus_tag="ACGE36_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFF8384819.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3213..3413
                     /gene="rpmI"
                     /locus_tag="ACGE36_002469"
     CDS             3213..3413
                     /gene="rpmI"
                     /locus_tag="ACGE36_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFF8384820.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3466..3825
                     /gene="rplT"
                     /locus_tag="ACGE36_002470"
     CDS             3466..3825
                     /gene="rplT"
                     /locus_tag="ACGE36_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFF8384821.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4077..4537
                     /locus_tag="ACGE36_002471"
                     /pseudo
     CDS             4077..4537
                     /locus_tag="ACGE36_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
BASE COUNT         1489 a          790 c          956 g         1302 t
ORIGIN      
        1 gagctggagg taaatgagcg tgatcgtttt agcaggtaca attggtgccg gaaaatcaag
       61 tttaacagaa atgatggctg aacactttga tagtcaggca ttttatgaat cgattgatga
      121 taatgaagtc ttgcccttat tttatgcaaa ccctgaacag tatgcttttt tgctgcaaat
      181 ttattttttg aataaacgtt ttgcaagcat caaacaagca atgaaagatg acaataatgt
      241 attggaccgt tcgatttatg aagattcact gcttttccat ctaaatgcag atctaggaag
      301 agcaacagaa acagaagttt gtgtgtatga tgaactgctg gaaaacatga tggaagaact
      361 tccctatgct gcccataaaa aacatccgga tttattagtt catattcgtg tatcttttga
      421 tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat gaacaattgt cctttgaccc
      481 aagtctatac gattattaca aagaattgaa cagacgttat gatcaatggt atgaagaata
      541 caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat aattttgtgg aagatcctca
      601 agcaaaagaa gaagtcttga aaatgatcga agaaaaatta gcagagattc gccaaaccaa
      661 agtatccatt tgatttacag ttgctcaaat aaagggtaaa cctttatttg agcgactttt
      721 ttctttatac tggaaacaaa tgtcaggagg atagagtatg cgggaaaaac actattttaa
      781 tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat gtgctagctg ttaaaaactt
      841 aaaagaagaa tatggcatta gtgacgagat gctgacttat tctctagata aaaatgaacg
      901 ggcgcgcgta gagtacgatt cttttgatga agcgttactg cttgtatcaa atgttccgca
      961 ccagcaaaaa gtcgataatc attatgaaac tagtcctatc gcttttattt taaaagaaga
     1021 cggtttgttt acatttacca cgccgaatac cgagtacgtg attcgactaa ttcgatcgct
     1081 tttggaacgg atgccagaca tgagtgtata cagtttattg tttcgtacat tgtttttgat
     1141 ttctgattcc ttttttcctt taatcgagga agtgaatagt gaacgtcagc ggcttaatct
     1201 gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa ctttcagatt tagagatcgg
     1261 tttggtttac ttagttacag gaacaaaaca aaatgtagta cttttagaac aaatcaaggc
     1321 actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa gagttggacg atgcactgat
     1381 cgaagcacga caagcagttg aaatgacgaa cttagcttcg cagattttag accagctctc
     1441 tggcacttat aataatttgc tgaataacaa cttgaatgac acgatgaaat ttttgacggt
     1501 ctggtcgctg atcttgaccg ttccaactat tgtgacagga ttcttcggaa tgaatctcca
     1561 gttacccttt acccattccg ttttcggatg ggaaatcgcc ttgatcatca gtttggtctt
     1621 atctatttgg atgcttattg cactgtggcg gcggattcgt tgatctagtc tttgatctgc
     1681 aaaccaaggg cttgggcgat cacaagtgct tgctcttgat tgacgattag tcccgccaaa
     1741 gaatcttgag ataaagcgat tgttgtgaag aaattagtcg taaagtctaa tcccttcaga
     1801 gaagttcgaa accagttcga accggttaac tgattgtcag acaaaaaaag gtttttccaa
     1861 gtgacctcat aaaattcgct gtcttctaat tgacaatgat cgaaggagac agctttcaag
     1921 tttgtactac taaaggaagc catcgaagca atacaatcag tgaaagtaca gtctcgaaga
     1981 taactttcgg caaaattcgt acctgttaat ttacattgat gaaacacgac ttggtggaaa
     2041 ctgccaccaa tccactccag attggaaaaa tcacattttt caaaaatgac attactgcat
     2101 tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt ggatatgact ctctcttaat
     2161 accatatttt tggcatcgag atagctttgg ttacctccgt tgatcagaag accagagaag
     2221 atctcctcat cttctaaata aaactctccc tttgtaagaa gaggaagaga aggtggaata
     2281 ggttcgattt ttttcatgtg ggttcacctc gtttttcttt agtatagcaa agaagcggga
     2341 atcactgcta gaaagcctgc taatctaatc attttcagaa aaggaagaca aactgtatga
     2401 aaaacattga caaaaagatt agtttttgct atactataat ggttgagaaa taaaagcaga
     2461 agcacccgct tctcgcctta gttgataatg tcactgggct agatagtttt cactggttct
     2521 attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt tttttattgc cgtttattcg
     2581 gtaagctttt ctttctctca taatatttgg aggtgaatga ccatagcaaa ggatatgatg
     2641 gttaacgacg gcattcgtgc acgagagtta cgattgatcg gttcagatgg tgagcaatta
     2701 ggagttaaga caaaagcaga agcattgaat attgcagaac aagcaaactt ggatcttgtg
     2761 ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca tggactacgg taaataccgt
     2821 ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac aaaaagtgat caatgtaaaa
     2881 gaagttcgct taagtcctac gatcgacttg aatgacttca atacaaaact tcgtaatgca
     2941 cgtaagttct tggaaaaggg cgataaagtg aaagcttcta tccgtttcaa aggccgtgcc
     3001 attacccata aagaaattgg tcaaaaagtc ttgaatcgct tagctgaaga aactgcggat
     3061 attgcaacag tggaacaaaa agcgaaaatg gacggacgca gcatgttctt gacgctggca
     3121 ccgaaaaacg acagtaagta atttgaatga ctaactggct gttggacagc cgatagtcgg
     3181 ataacgaaag attttgagga ggaaatatag tcatgccaaa acaaaaaaca caccgcggat
     3241 cagcaaaacg tttcaaacgt actggtaaag gcggattgaa acgcttccgc gcgtttacaa
     3301 gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca attgcgtaaa gcaagcatgg
     3361 tttctagtgg cgacttcaaa cgtattcgcc aacaactagc aaaaatgaaa taagaagtac
     3421 aaataacaga atctgaagat ttatttttat taggaggaat tatacatggc acgtgttaaa
     3481 ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa aattagcaaa aggttactac
     3541 ggttcaaaac acactttatt taaaacagca aaagaacaag taatgaattc atacaactac
     3601 gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta aattgtggat tgctcgtatc
     3661 aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat taatgcacgg tttgaaagtt
     3721 gctgaaatcg acatcaaccg taaaatgtta gctgacttgg ctgttaacga tgcagcagca
     3781 tttactgcat tggctgacca agcaaaagaa gctttggcta aataagctaa acaattataa
     3841 agaggaaaga agataacgaa aagttatgtt ctttcctctt tttttattta caatcctcca
     3901 tcaaatgaaa caggacaata acgtataagt ctaaaataac ataattaagg ttttttaatt
     3961 gaaaactgaa catttatggt catcctttaa tgaaagcgct aactacataa gttagaatga
     4021 gtttgactga tatattagtt tgcttatcag aaaaacaaaa tggacggagg aataacatga
     4081 gtgatattat tattggtatt ttgttagtta tctcattctt tttcatggtc tggtactgtg
     4141 taaaaggcta caacttgatg gtcggctttt gtcatcatgg ctactgtatg gatgggatta
     4201 gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg aagggcaagg agtaattgat
     4261 gtgttgacgc atatctatac gacggggcca gctgagtacg ccaaatcgat cttggtaaat
     4321 gtcttctttg gtgccttttt cgggcgtgtt ctagtagata gcggaatcgc agctaccctg
     4381 attcgaaagg tagttgaact tggaggggac aagccaagaa tcacgatgag cttactatgt
     4441 attgtaaccg ctgttatatt catgtcgatg acaggaatcg gaccagtgat ttctattgca
     4501 gttattgtgt tgccgatctt gatgtcatta ggtattt
//
LOCUS       DAXWLH010000184         4531 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXWLH010000184 DAXWLH010000000
VERSION     DAXWLH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.189"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            26..2749
                     /locus_tag="ACGE36_002472"
     CDS             26..2749
                     /locus_tag="ACGE36_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8384822.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            2762..3394
                     /locus_tag="ACGE36_002473"
     CDS             2762..3394
                     /locus_tag="ACGE36_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8384823.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            3602..3826
                     /locus_tag="ACGE36_002474"
     CDS             3602..3826
                     /locus_tag="ACGE36_002474"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFF8384824.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            3928..4161
                     /gene="relB"
                     /locus_tag="ACGE36_002475"
     CDS             3928..4161
                     /gene="relB"
                     /locus_tag="ACGE36_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8384825.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            4151..4420
                     /locus_tag="ACGE36_002476"
     CDS             4151..4420
                     /locus_tag="ACGE36_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8384826.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1596 a          684 c          896 g         1355 t
ORIGIN      
        1 acatattgtg aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac
       61 attgcttttg aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct
      121 acagttgaat agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt
      181 cataaaagta aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca
      241 tatcacactg aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa
      301 acccgcatta gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat
      361 taaaacggca gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg
      421 atcagggaaa acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat
      481 tcacacatcg gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt
      541 actatcctct gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca
      601 tactggtttg ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt
      661 atctgaagaa gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat
      721 gggggataat agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa
      781 tatatatacg actttcttac caacttttct taggagattg ccagttattg ttaatctacc
      841 tagatttcaa caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc
      901 tgagagccaa attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat
      961 gaatagtgat tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg
     1021 aaacgcatat atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc
     1081 tttagagtac agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga
     1141 tcgaatctac tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt
     1201 tgttcaattt tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga
     1261 tattaaacta tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat
     1321 ttttcaagag gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg
     1381 tcaaagtatt gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt
     1441 cgctttggcc aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca
     1501 agaaaagaaa accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta
     1561 ttggtatgca aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga
     1621 agaggacatt ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac
     1681 aggtattaag agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt
     1741 tgccaaccgt atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac
     1801 attagagaaa attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg
     1861 cttgatttta ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca
     1921 tctaaatgga ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg
     1981 ccaacaagta ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct
     2041 tactttcaaa aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg
     2101 ttgttataca gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa
     2161 agatgcagtt agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa
     2221 gaattttgag actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc
     2281 caaaattaat cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg
     2341 cgaatttgtt cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga
     2401 attaattttc aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga
     2461 taaggtgcta tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga
     2521 tttttctaat aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat
     2581 tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg
     2641 agaaataatg gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga
     2701 aataccagat ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg
     2761 aatgacaatg ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta
     2821 ttttgagaga agaatgaact tctttcaaga actggagata gatccaggat caaaccagat
     2881 tgatgattat gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa
     2941 atcattaaga gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga
     3001 tttcgctaaa gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga
     3061 tataccattg gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca
     3121 atgtgaactt actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa
     3181 accacatccc gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc
     3241 agtagaggat tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc
     3301 actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca
     3361 agaactggaa caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata
     3421 tctgtgctag ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg
     3481 tgctggtaac accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt
     3541 tttttatttt gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat
     3601 tttgcaacag aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt
     3661 aggaggggat actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg
     3721 gggtgaagtg atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac
     3781 gatcagtcag agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat
     3841 gcaaatcttg tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta
     3901 gtacaaatga tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc
     3961 gacgatgaaa aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa
     4021 ctttatcgaa atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg
     4081 agtgcaattg aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag
     4141 aaactgctaa atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa
     4201 aatggacaga aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac
     4261 gactgacccg cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta
     4321 tcgaattgga aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat
     4381 attctcaatt ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag
     4441 agtttcgaaa gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac
     4501 ccgatactat cggttggttt ctgctttttt a
//
LOCUS       DAXWLH010000185         4513 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXWLH010000185 DAXWLH010000000
VERSION     DAXWLH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.190"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            137..451
                     /locus_tag="ACGE36_002477"
     CDS             137..451
                     /locus_tag="ACGE36_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8384827.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            453..2108
                     /locus_tag="ACGE36_002478"
     CDS             453..2108
                     /locus_tag="ACGE36_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8384828.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2191..4326
                     /locus_tag="ACGE36_002479"
     CDS             2191..4326
                     /locus_tag="ACGE36_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFF8384829.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
BASE COUNT         1586 a          765 c          953 g         1209 t
ORIGIN      
        1 taccccttat cggtatttta tgcaaatata aaaagtagtg aaaaaaatga agagtcattc
       61 tggttgacat attaataaag ctcctgttta atatacccca tgaggtattt ataaaaatgt
      121 aaatgaggtg cagaaaatga atttgctatt tcaatcaata aaatcgataa cagtcccaga
      181 attaaaggaa aagcttttgg aaaatccagc gttacttgat gttagaacac ctgcagaata
      241 ccggggcggg catataaaag gagctaaaaa tgttccatta caaagtataa acagatatga
      301 tggagacaaa gaaaagaccg tctatgtgat ttgtcaatca gggatgagga gtaaacaagc
      361 agctaaagaa ttaaagaaat ctggttatga tgtagtgaat gtacgtggtg gcatgaatca
      421 atggtttgat cgaacagtag gagggaaata agatgaaaat cgttattgtc ggaggtgtag
      481 caggtggaat gtctgctgct acacggcttc gccgattaat ggaagatgca gaaatcgttg
      541 tttttgaaaa aggaccgtat gtttcttttg caaattgcgg cttgccttat tatctttcgg
      601 gagaaatcag tgaacgggaa aatcttcttg tccaaacgcc agaatcatta tccgctcgtt
      661 tttgtttaga tgtgcgtcca aatcatgaag tgacagccat ctttcccgaa aacaaaacgg
      721 tagaagtcgt acatgagggt caaaaacaca ttgaacagta cgatgcattg gttttatctc
      781 ctggtgcaaa accagttgtt ccatcgattc cagggataac agaagccgac aatgtttttt
      841 ctattagaaa tgtaccagat atcgataaag tgatgcatgc attagaaaaa cagccaaagc
      901 gtgccgtgat cgttggtgca ggattcatcg gattggaaat ggcagaaaac ctaaaaagaa
      961 gaggtttaga agtcatggtg atcgaacaag caccacatat tcttccaaca ctggatgaag
     1021 aaatggcagc ttttatagaa aaagaattgt ctcatcaagg agtagaagtg attacttctc
     1081 atgctgtcgc tggatttgaa gaccacggga aacgattgcg actggatgat gggcgtacca
     1141 tccctgctga tttagttatt ttatccattg gtgttcgtcc tgataaccag ctagcagtga
     1201 ccgctggaat cgaattaggc atacgcgggg gtatcctagt agacgaacgt tatcaaacga
     1261 atattcctga tatttatgcg gtgggggatg ctatcgttgt aaaacagcaa atcactggaa
     1321 aagatgcact tatttctctt gcttcaccag ccaatcgtca aggtagacaa gttgcggaca
     1381 cgatttccgg aatttctcga agaaatcaag gcggtattgg aacagcaatt atacgaacgt
     1441 ttggaatgac tgccgcatcc accggtttaa gtgaaagaac agccaaagaa aacgaactgt
     1501 cttttgaagt cattcatgta tcaggaaaag atcatgcaag ctattatcca gaagcaacag
     1561 atattttact gaagttgatc ttccatccag agactggcga gatttatggt gcacaaggtg
     1621 ttggggcaaa aggtgtggat aaacggatcg atattttagc aacagcaatc aaagggcatt
     1681 tgacgatctt cgatttaccg gaattagaat tgacgtatgc accgccattt ggctcagcca
     1741 aagatccagt aaacatgcta ggatatgcag caatgaacat tgcagaaggg cttagtgaaa
     1801 ccgtacaatg gcatgaattg ccgacagaac tagcaaaagg aaaaatttta ttagatgtgc
     1861 gaacagcaga agaattggaa aagggcaaat tcaaggaagc caaacatatc cctttgaatg
     1921 aacttcggga ccgattaaat gaattagaca gccaacaaga atatatcgtc agctgtcata
     1981 gcggattacg tagctatcta gcggaaagaa tcttgaagca gtctggctac cacgtaaaaa
     2041 accttgatgg tgcattttct ttatatcaaa ctgtccgacc tgaagaactg atatatccta
     2101 acaaatgata ctcgttaaaa agcttgcatt cgactcttaa aagaggtaaa ctaatcggtt
     2161 ccaccacttt aaaaaaagag gagaatgtgt atggtcaaca cacaaaaaca gttagaagaa
     2221 gcatatctga agaacgtgta tcaaaagcta attgataaaa aaaacgaact gcgggaatcg
     2281 atggatacgg caaaaataga aggaaaccag tcgttgaaag aaatggctgg cgagcttcga
     2341 ttgaattttg acagttactt agataacctg gatacttttt cgatgattga aatgaaaaat
     2401 agagaaatcg atcagatgaa tatcaaacag aaaaacgcag aaaatcagct agcaaagatc
     2461 gaacgtttat tgaaaagtcc ctactttgga aaagtcgttg tagactttct ggataatgag
     2521 ccaaaagaat ccttttatat cggtacagca aatttcacag atgatgcaga tgaaaatctt
     2581 gtttatgatt ggcgttcacc aattgccgaa cttttttaca ataacatgat tggtccatct
     2641 tcttatacgg ttcgtcaaaa taaaatcgat acggttatta aagaaagacg acaatttctt
     2701 ttggaaagag atcgtttggt caagtttttt gatacgtctg tagctattca agatgacgta
     2761 ttattagaag tcttaggcca agatgagacc aatgaaatga aggctatcac tgctacgatc
     2821 caatcggaac aaaacacaat cattcgggat gtgaaaagtc aaaatatctt ggtaaatggc
     2881 gtagcaggaa gtggtaaaac ctctgctatc atgcagcgta ttgcgtatct gctctattta
     2941 tatcgagata cgatgacatc cgatgacttg ctgattctat cacctaatca tcggtttatc
     3001 gactatattt cgaatgtcct accatcatta ggtgaaagaa atccactgaa tctaaccatc
     3061 atacagctgg tttcacagct ttcagcagag gaaatagaag gtgaagaggc ttatttcaaa
     3121 cgtatcacgg gagaaaatgt atcggagcag acagaacgtt tgcgtagcaa aaagtttatc
     3181 gataatctaa aacaatcaga tcctttgttt ttagatcatc cgaattttat ccgtggactt
     3241 actaaaaatg gaaaaacagt cctttctaaa aaaacgatcg aaaaaatata tgagaaagtt
     3301 cctgctcatc ctaaattgat cgataggctt caagcaacga aaaaggcact gatgagcgaa
     3361 tggaagaatc atcttttgaa acaagcaaaa agtcctgctg tgcagaatca ggtactctcg
     3421 ttgacagaag ataggcagtt ggaattattc ggtaaattga tttctgatga ttcggaacaa
     3481 agtattgtag cttatgcaag gaaactttta caaaaaaaat atcgaaaaat aaccagacaa
     3541 atcgaagaaa tggcatgggt ggatgagcat cagttgtttg aaagaattta tgaaaaacga
     3601 tatggctcag cttatgcttg gcaacccaca cgtactgttg atgaagcagt cattatatta
     3661 gctattcgtc atttacttgt tgaaaaggta aatgtaccag catttcgcta tctattgatt
     3721 gatgaagtac aagattatac cctggcacaa ttaggtctat tgatcgaatt atttccaaaa
     3781 actcacttta ctttagtagg tgacgagaat caggcaatct ttaattcttc cactactttt
     3841 gctgatatta tgcgttgttt cgacgactac catctgccga tccatcggta tgatcttaga
     3901 aatagttacc gttctagcgg agcaatcaca gagcttttca aaacctatgc tgtggatcaa
     3961 gagaagatcg acatcgtatc gatccgaccg caaggaaaag aacccgaata ccgctctttt
     4021 cgatcctctg aagaattgtt gaaaatacta gctgagattt tggtttctct aaaaggagaa
     4081 aaagcggcag ttatcaccca aacagaagag gaaacccaaa cgctacaaga atcgattaaa
     4141 aaaaattcat atggattaga aaactgccaa atcattcctt tgagtctagc aaaaggactg
     4201 gaatttgatc atgtaatctt atatccattt gaaaacgatg gagatgagca aagaagacgc
     4261 agacaaatgt acacggcgat atctagagga atgaaaagca ttgtcgtatt ggaaagagca
     4321 acataattaa gagataaaaa ataaaaagaa caactgataa aaagaacaaa atatttttca
     4381 tttgctgatg agaaatgttt tgttcttttt cttaggaaaa atttaggaac actttatgtg
     4441 aaaagaaatg aaatgtatgg taaactccta cgtcgtaaca aaaaaacgtg aggtgaggat
     4501 atgaaaaaag taa
//
LOCUS       DAXWLH010000186         4498 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXWLH010000186 DAXWLH010000000
VERSION     DAXWLH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.191"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(423..1235)
                     /locus_tag="ACGE36_002480"
     CDS             complement(423..1235)
                     /locus_tag="ACGE36_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384830.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1398..1505
                     /locus_tag="ACGE36_002481"
     CDS             1398..1505
                     /locus_tag="ACGE36_002481"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384831.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1575..2630
                     /locus_tag="ACGE36_002482"
     CDS             1575..2630
                     /locus_tag="ACGE36_002482"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8384832.1"
                     /translation="MQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFEELD
                     YAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQFFRK
                     VLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNESNG
                     ETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKIISK
                     THDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSFEKT
                     MSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQEEV
                     EYYERKLQSISNEKTSY"
     gene            2772..2975
                     /locus_tag="ACGE36_002483"
     CDS             2772..2975
                     /locus_tag="ACGE36_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8384833.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3035..4237
                     /locus_tag="ACGE36_002484"
     CDS             3035..4237
                     /locus_tag="ACGE36_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8384834.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1491 a          718 c          945 g         1344 t
ORIGIN      
        1 ggtaatttgt atgacatgta ctgatgaagt atttcgaaaa catttttaaa ggttgagatg
       61 tatatagatt taaccaatca tttatctctc tcggaaaagg aatattaact aaccagcggg
      121 ttatcaaaaa taggtaggaa aactgtactt aatggcttat ttcgcgctga ttatgagaat
      181 atcagtctgt ttgattaatt tttagggttc aaaatacaga gacaaactcc aggtcgggtt
      241 tcgccacaag cgaattaccc gatctggagt tttgtctatt gcaggaaggc gtagccggac
      301 tgcaggaata aagcccccgg ttctaacagg gggctaacaa agaacaggaa actgtgacaa
      361 ctacccattg gggagtgtcc tagcagtatt ttctttgtca cgtgcttatc aattttgtca
      421 cctcattttg atgatggata cgctgcattt aacaggcgtt ctttctcgac ttccatcacg
      481 aatggtagat aaagatattg ctgttctagt agatattgat tgtcctctgt gtactgataa
      541 gaagcggttt cttctgaata gaccaacggg gcaattcgca catcgctggt caattgatta
      601 attgagtata gaaggatggc tttctcacct agttccttgg gtaaatgttt cccaaaatag
      661 gaataggatt tttgatttgc taaatagacg agtatgcgat ccacactaaa gttggagaag
      721 gtaaagggca tcatgtgcag aatatgattt tgattatcat gttgcaaata gagctgggct
      781 tccttaccaa aattagttga accaagataa agctgctttt tttcatcgat acattggaaa
      841 gtaaactggt cttgttcagt tggtggaatg agatttgtaa taaaaaattc aatcattcga
      901 tccatttctt tttcactaac aaggtcagcg tgtttgattt cgtctactat gagaccttca
      961 atcttaggat caagcttacc tgcctttttt tggagcattt ctaaaatgta ttgattctct
     1021 tgatcaccat acaaaagata ttcattggtg acatgaccta tctctgcgat ttggtgaagg
     1081 gtttcggttt ttggaaggtt gatcccacgt tcccagttat tgactgtact tcgcggtaaa
     1141 tcaccaatta gtttgccaaa tttctccata ctcaacttta attcttggcg aatctgccga
     1201 attcgattgc cgactgcttg cttgtctatc tccatgaaaa ctcctcctca ggataagttc
     1261 ttaaatttag tatacgtgtg tacttaaaaa aagtcaaaac atattgactt aaaataaata
     1321 caggcgtaca ataaaaatgt acttaaaata agttcaatag gttctttgac aagtaaatag
     1381 cagaaggagg tttgtcgatg aaccaagtta atcaacatga tctcggtgaa gccattcgag
     1441 tttctcgaga agaaaggggg tggacacaac gatatttagc agaaaaagtg gggatttcga
     1501 gatagttatt atcgaaagta gaaaaaggaa cgagacaatt aagtgaagaa aaattgaatc
     1561 ttgtgttgga tagtttgcaa gaagaggtcg ttccagttaa ccgtgttttg attgattatc
     1621 taaccattca ctttttctcg aatcaacatt taaagctaat tgaagagatt attgaaatgc
     1681 ccattgaacg ttttgaggaa ctggattatg cgccaaaggg ctatattggc caatacgtgt
     1741 ggaatcaagt aatcacgatt cggtattcca ttgacgatgc ggttaaagga actgtgatgg
     1801 agttttcagg tcaaggctgt aaacacttag caagatggct aaaaacacga aaaacaaatt
     1861 ggcaacagtt ttttcgtaag gtattagatt atcaggggaa ctttacacgg attgatttta
     1921 ctttagatga ttttgtgggg agtctcagca ttccagaact taagcataag gtaatactag
     1981 gccatgtgtg gaccactttt caagtgagtg aatctcatgg cggtacggat atcataaaca
     2041 atgaatcaaa tggtgagacc ttgtatttag gctcaaagaa gagtcagtgt cgtttttgct
     2101 tttatcagaa ggaccatgaa caacggaagc gacgaggaat tcctttagaa gaggcaaaag
     2161 taaagaatcg ctttgaacta cggtatcgaa aagagaaagc gcaaagcttg gcaaagatta
     2221 tttcaaaaac ccatgattta accaaactgt tctttgagtt acttaatgga gcaatttgtt
     2281 tttatgaccg tgatccaaat gatctaggtg caaaagttga tgccaaatgg gcagccttta
     2341 ttagcaatta tggcgcaatc accatttctt tagatacaat tcctcaaagt tttgagaaaa
     2401 ctatgagttg gttaattcat ggtgtttcac catcattagt ttttattgca caggtcgatc
     2461 aaacgtttca ttcttccttg ttgcctttga ttattgaaca aggagaaatg aatccaagac
     2521 agatcaaact tctccaaaat atgaaggcag acccggagta ttatcaggag gaggttgagt
     2581 attatgaacg aaaattacaa agtatatcaa atgagaaaac aagctactaa aaaagcaaag
     2641 tactgcttac acaacaagtt gtgcaacgta ctccgcctcg acaagacgag tataacacaa
     2701 ccatcgaatg agaaacaata gattataaag aggattattt taaaaggagt aaagaatcag
     2761 gaggtgagag aatggaaaca aaatgtgatt tcatggtaaa tcgtgcaatt ttgatagaga
     2821 tgggctttaa acctagtcaa gctgcacgaa tgattaaaga gagcaaggca tatcttgcaa
     2881 ggattgaagg tattgatttt tataataatc gccaagttgg tgtggtgcca tcccgagtaa
     2941 ttgaacatct ttttcacatt caagttgcgg aataaacagt acaagcgcgg tactattaaa
     3001 gtagcttttc tcaagtacag ggaggaatgg aacgatgcca aaaattaaaa atatttacca
     3061 agataaaaag acaaaaaaat ggttcttccg agcgtatcta ggtatagatg aaaatggaag
     3121 aaaggttcag aagactaaac gaggttatgc ttcacaaaaa gatgccaaaa ttgcttatga
     3181 caagtacatg gaaacacatg attttaacaa gacaattcct aatcaactgt gttctaccaa
     3241 tcaaatgact ttcgaagagt tttacaagga taggtttgta aaatggtacg agagacaggt
     3301 caaaagtcaa acatatgaga atgcgcaatt cattttcgaa aagcgtatgc agtttttcta
     3361 tcattttcgt gttcgagata ttacaagtca cgatattgag gattggttat ttgaactaag
     3421 ccagacagag tcacgtaatt cacggaaaaa agttaatggc gaaacgttat caaaatctta
     3481 tatcaatcgg attcgagggc acctaaaaat tgtattggat cgggctgtta aagagggggt
     3541 gattgctaaa aatcccgttg aagatgtttc ttcgcttcct tctgagaata agaaagttga
     3601 gttttgggaa cacagtgagt ttcaaaaagt gatgaatgaa ttgaaggatg ataagattca
     3661 aacccaccat cggaagattg tgtatgaaat gctcttttat actggcattc ggattggaga
     3721 acttgaagct ttgtcttggt cgaatgtaga ttttgagaaa aacacgataa cgattgagaa
     3781 gacattgatt tataatacaa aagatgattg gtacttttcg actcccaaaa ctaaatgtgc
     3841 ttatcgaacg attgggttgg ggaagaagct ttccttaaaa ctcagacagt ggtatgactt
     3901 gcaaaaacgt attggaaatt ttgaatatgt tgctcagttg gacggaacat ttacgccacc
     3961 ttattctttt gcgaactggt taaaagaagc tgctaaaaaa gcgggtgtca aaccgattaa
     4021 gcttcacgcg ctacgacatt ctcatgttgc atttctaatt gagcagaata ttcaaccgct
     4081 atctattcaa gaacgattgg gccatgcgaa tattcaaatc actttaggaa cttacggtca
     4141 cttatatgca aagtcagatc aacaagtggt aagtgcgatt gataatgttc aagagggcgc
     4201 tattctttca aaaaatgaca atttattacc agtctgagtt ttccacggtt ctaccataag
     4261 acaaataaat gcttttgcac cagtgttttc agtcgaattg ctcctgttca agatgtatcg
     4321 gaaatagcga gaaaataagc aaatttgcat aactataata aaaaggactt agttttgatg
     4381 ggaaattttt caaaattaag tcctttttta tgtggttgaa acgggtatga tcattttaag
     4441 gaagtgatct aagatgaaag gacgactgaa caattatcat attagaaaag ctttattg
//
LOCUS       DAXWLH010000187         4462 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXWLH010000187 DAXWLH010000000
VERSION     DAXWLH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.193"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..392)
                     /locus_tag="ACGE36_002485"
     CDS             complement(<1..392)
                     /locus_tag="ACGE36_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384835.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFK"
     gene            complement(600..1526)
                     /gene="glsA"
                     /locus_tag="ACGE36_002486"
     CDS             complement(600..1526)
                     /gene="glsA"
                     /locus_tag="ACGE36_002486"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFF8384836.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1549..2976)
                     /locus_tag="ACGE36_002487"
     CDS             complement(1549..2976)
                     /locus_tag="ACGE36_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8384837.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3007..4221)
                     /locus_tag="ACGE36_002488"
     CDS             complement(3007..4221)
                     /locus_tag="ACGE36_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFF8384838.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1408 a          936 c          783 g         1335 t
ORIGIN      
        1 tctttaaaaa aatccaatac gatttttttg attgtatata ctttgctcaa agaaataaat
       61 tcaagatcag ccagttctgt actcgtgaat ataccattaa aataataact taaaaacttt
      121 aaaaaataag attgaccgta cagaaactgt gtgagctcag aaagagcaat accatgtttg
      181 aatacgagtt tatacttatg cttctctctt acttgttctt ttatacaata ttctagacac
      241 tcggctagac tttctagatc gtgaaggatc gttggtgtag tcacccttag agaaatagct
      301 gtttgttcga tgtctatttc ttggaattca aataattgtt cacacagata cacttttcta
      361 aatatatttc gctcgatgta attttccagc attcctgata cacgcctcca gtttttctta
      421 tgattttatt taatcatatc tgtaaatcgt atttttgttt cctttttatt tagaaaagcc
      481 ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc aaacaagctc acaatttcag
      541 cctcattgta ggtagctttg agccgtttgg acttttaccc cagcctcttt caagaatggt
      601 tattcaaaga tgtcagcgtc tagttctttg actacatctt tcaacaattg gataccagct
      661 gcgctgttac caaatggatc tagtgccggg ctgaagacac ctatgccgta acgatttggc
      721 acagctgcca tcaacccgcc gcctacaccg ctttttgcag gtacaccaac atggacggaa
      781 aactctcctg attcatcata caaaccagca gttgtcataa ttgatttgac gattgtagca
      841 ctttcttcag taatgatttg tttgccattc caaggagcaa ttcctttatt tgccagcaca
      901 gcagctaatt ttgctatgcc tgtagcggtg acattgaggg agcattgtct gaaataagca
      961 tcgagtatct ccggtacgtc tccttcgatc atttgattac ctttcatata gtaagcaaga
     1021 gagcgattga tatcacctgt tcttgattca gaatgataga tttcttcatt caatgtgata
     1081 ttaggatcat cacagatctc tttcataaat gacaagattc gctcaaattt ttcatctgca
     1141 tctttaccgg caatcaacga ggttgttgca atcgctcctg cattaacaaa aggattcatt
     1201 ggatgtttgc gatgattgat ttccatattc atgatagaat tgaaggcaaa accggttggt
     1261 tccgtgttga ttgtttgaaa gactttttct attccgttgt ctttgattgc taataacaat
     1321 acgggaactt ttgacatgct ttcgatggca aagcgcacat cggcgtctcc tccctcaatc
     1381 agttgttttg tatccaaatc ataaatcgcc atacctaatt gtttgggatc cacttcagct
     1441 aatgctggaa tgtaatccgc cacttgtcct gtttgataat agtggaaatt ttttccaatc
     1501 aattgttcta attcttttac ttccatttga ataaccagct ttcatttttt atttttctgc
     1561 atgctggaat ttatgacggc atgcgtagat cgtaaatgga ataactaaga cgataagata
     1621 agaaacaatc agaatcgttt cataaatccc atgctgtcca gctgcgatac tagcaggtgg
     1681 aacaaatgag atacaaaaag caaaaagtga agtcaaaaga cctaaacaag caacgattac
     1741 ttttacaact tttccgcctg gaacctgata ggctctttca agactgtcat ctttaaaaac
     1801 cagcttcaaa tagcccgcga aaaacataaa gtacaacatc aggtagatta caacagtcaa
     1861 tgacatcgct gcaagaaatg aaagattatt ccccccgccg cttaatgtaa agacggctgc
     1921 ccagattgtt gcaacgatcc catttgcgat catcaaatga gcaggaacac cgtgtgtgtt
     1981 ttcttttgca aaatattcag gaagtaatcc ttcttctgct gctacgtgaa gtgcttttga
     2041 aggagaggtt acccaagaag caatttctcc gatgacacct aaacaaacaa gaatcgcaag
     2101 aattttcgca atccatgcaa gatttggatc aatgtgtctt aataggaatt ctaacgattg
     2161 aatcacccct gtattaagag acaaaccatg ttgaggtact gtagtagcga cggtcaatcc
     2221 gccaaaagta tctaaaatga ttgccagaat aaccaataca aacattgcta atggataatc
     2281 ctttttaggg ttttccattt catttgcatg actggcagat gtttctacgc ccatatatgc
     2341 taaaataaag gaaacaaaaa caaccagcgt aggcaatttc gaaaaatcag gaatcaatgt
     2401 tttcatagac agtatcgttt gtagtggagc gcctgaagcg acataatgga tccctaagcc
     2461 aaacaagact aatgctggca acaagatacc agctacaaaa ccgatccgag caatcaatga
     2521 agtgtatttg gttcctttta actgtgagag taaaatagcc cagaaaataa tcaataggat
     2581 tgccaatttc agatatttat tgttttgtat agcaccaata cccgttgcat aggataatgc
     2641 accagttaag aaatacagca tggtcaagta gccaactgtt atctcaaacc attggaaaaa
     2701 gattgcggca aaaccgaatc gttttcccaa tgtgcgactg acccaagtat agacaccgcc
     2761 tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag ggataaacca
     2821 tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg cgaatgtggg
     2881 atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa agaacgttaa
     2941 tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt tctatttgaa
     3001 tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc
     3061 ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg ttgtcagcac
     3121 ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga agtaccagct
     3181 gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt
     3241 gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt gtttcttttt
     3301 acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca gcatggcact
     3361 ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa cggatcctgt
     3421 tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca gccaagtaat
     3481 catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt gttcatctgc
     3541 tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat
     3601 agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct ttctttgacc
     3661 aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga ctgctccacc
     3721 agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc agtggcaaac
     3781 agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt ttaaattcaa
     3841 tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca tcagttggta
     3901 aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga agcttgtaac
     3961 atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat cccgtccaaa
     4021 ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg agagaaaaga
     4081 ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa aaaaagctag
     4141 accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg tatctcctgt
     4201 attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag tataaaagaa
     4261 ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat aaaagacaga
     4321 caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa taaagacaaa
     4381 aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt atttttaaag
     4441 atattttttc ataaggaata aa
//
LOCUS       DAXWLH010000188         4306 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXWLH010000188 DAXWLH010000000
VERSION     DAXWLH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.194"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            85..354
                     /gene="rpsN"
                     /locus_tag="ACGE36_002489"
     CDS             85..354
                     /gene="rpsN"
                     /locus_tag="ACGE36_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8384839.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            369..548
                     /gene="rpmF"
                     /locus_tag="ACGE36_002490"
     CDS             369..548
                     /gene="rpmF"
                     /locus_tag="ACGE36_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8384840.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            580..957
                     /locus_tag="ACGE36_002491"
     CDS             580..957
                     /locus_tag="ACGE36_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384841.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            978..1127
                     /gene="rpmG"
                     /locus_tag="ACGE36_002492"
     CDS             978..1127
                     /gene="rpmG"
                     /locus_tag="ACGE36_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8384842.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1151..2134
                     /locus_tag="ACGE36_002493"
     CDS             1151..2134
                     /locus_tag="ACGE36_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8384843.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHREERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKHYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2164..2286
                     /locus_tag="ACGE36_002494"
     CDS             2164..2286
                     /locus_tag="ACGE36_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8384844.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2304..3827
                     /locus_tag="ACGE36_002495"
     CDS             2304..3827
                     /locus_tag="ACGE36_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8384845.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            3851..4090
                     /locus_tag="ACGE36_002496"
     CDS             3851..4090
                     /locus_tag="ACGE36_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384846.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSV
                     DTVHRALKKDPDCCFIKVNTGRSRRLAKRYNILIN"
BASE COUNT         1627 a          619 c          862 g         1198 t
ORIGIN      
        1 ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta cgatttgaga tgtctaaatc
       61 aaaataaaag gagtataaag gtatatggca aaaaagtcga aaattgctaa agcaaagaaa
      121 caaatggcga tgattgaaaa gtacgcagat aaacgtcagg aattaaaagc cgcaggagat
      181 cgcaccgctc tcgctaaatt gccaagagat tcaaatccga atcgtctgag gcttcgtgat
      241 cagacggatg gtcgtccaag agggtatatg cgaaagttcg gtatgtcccg tattaagttt
      301 agagaacttg cccatcaagg gttgattcct ggagtaaaaa aagcaagttg gtaaaaggag
      361 gaaatatcat ggcagtacca gcaagaaaaa catctaaggc gaagaaacgg ttaagaagaa
      421 ctcatcaaac tgtagtaaaa ccagaaattt catttgacga agcgaatggt gattatagac
      481 gtagccatca tgtatcttta aaagggtact ataatggaaa gaaagtaatt aaaggagctt
      541 caaaatagtt taatatataa cttttaggag ggaatcaaaa tggaaattat ttacccacct
      601 ttagttgaag aaggattaaa gtattatctt gaaacgaccc agcaatcgct agataaatgc
      661 actttttatc gttcgatggt agagagagga attattactg aaacgggttt accgacccaa
      721 caggcaattg aaaatggatt agtcaaagat tactatgagg accaaggctt atcatttgat
      781 gattttttaa gaatctatcc aatttttgaa gagtatgacg aggagttatt tcaatgtatt
      841 gatggttatt gggagatacc tgttgatatg aaagaaaatt tagtttcaca attggaatct
      901 ggcgagttaa cttttgaaga tgcacaacaa attcaagcat atcttgaaga tcgataagta
      961 ttgaaggagg aagatttatg cgtcaaaata ttattttgga atgtgtcgaa actggagaac
     1021 gtctgtatct caccagcaaa aataaacgga ataaccctga acggttagaa ttaaaaaagt
     1081 attcgccaaa attgcgcaga cgggcaattt tcaaagaagt gaaatagcta agtggtaaaa
     1141 aggtgacaaa atgggcatac cagttacaat cgtttctggg tttttagggg ccggaaaaac
     1201 aacgctaatc aatcaagcct tgcaagtctc accctacccc agggaagaaa ttattataat
     1261 tgaaaatgag tttggtgaag ttgggattga tcacaaatta ttagttcata gagaggaacg
     1321 ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt cgcagtgatt tattagcaat
     1381 attgatttct ttactggaaa caatagaaga acatcaacaa ccaatttcac aaatcatcat
     1441 tgaaactaca ggtatagcag atccccaacc aatcattcaa acacttttaa cagcgcctca
     1501 actaagagga agagtatata tcgatagttt attgactgtt attgatagtg acagttttga
     1561 acttattgaa agagaaccac aagcccttaa gcaacttgtc atggcagatc gcatttttgt
     1621 gtctagaaaa caaaacttga atttatctgc actaaaaaaa ttacagagag aaatcaaaag
     1681 tattaaccct ttatccgata ttcgaatgtt cacgcatagt gaaacgattc aaaaaaagga
     1741 tttttataat ttgaaaaaat ttaatcatcc cttagttttt gaaagtgatt catcagaaaa
     1801 tcactataat ggcaaacact atcatcaaca ccatcaagta catgagttta aatcaattct
     1861 cttaacttca gatcaagata taaaaaaaga ttttttactc caatggattg actggctgat
     1921 tttaaccaac caagggagcc tgtatcgttt taaaggattg attgctttac aagagcagga
     1981 ttttatagtt gccatgcaag gggtaaacga acaggttaat tttcaataca ccactcgaca
     2041 aaaagaccaa accactatta ttctaatcgg aaaaatgttg gatgaagaaa ggatcaaaca
     2101 gagttttgat aaattactgg cggaaagcaa ataatgataa gtattgattg ggggtaattt
     2161 ctaatggaga aaacggatat atctagtgca tatcgacgac ttaaaagccc gaatattaaa
     2221 actagaaaaa gggcattaaa aattatcaaa gaacataaac gaaataaaat gaagaaatta
     2281 gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt aggacttatt gggatagcag
     2341 ctctaggatt attgggagct tgctccagca ctaatgatgc aaaagtatcg aatgataaag
     2401 atggaaaatt agaaattgta acaacattct atccaatgta tgatttcacc aaaaacatcg
     2461 tcggcgatga agcgaacgtg gatttaatgg ttccagcggg atcagaacct catgactatg
     2521 agccctcagc taaggatatg gcaaaagctc atgatgctga tgtttttgta tatcataacg
     2581 aaaacatgga atcatgggta ccaaaagcta aagaaagttg gaagaaagct gggcctaatg
     2641 tagtagaagg aacaaaagac atgattttat tgccaggtag tgaagaagaa gatcatgatc
     2701 acggagaaga agatcaccat catgaactag atcctcatac gtgggtttca cccaaaatgg
     2761 caattaagga agtttcaaat atcaaggatc agttagttaa attgtatccg aaaaaagcaa
     2821 aagtatttga gacaaatgct gaaaagtatt tgactaaact gaaacggttg gatgctgact
     2881 ataccactag tcttaaagaa gcgaaacaaa aaagttttgt gacacaacat gcagcctttg
     2941 gatatttagc tttggattat ggattgatac aagttcctat tgctggacta agtcctgaag
     3001 aagagccatc ttctggtcga ttagctgaat taaaagagta tgttaaaaaa aataagatca
     3061 attatattta ctttgaaaag aatgctaatg ataaaatcgc aaaaactttg gccaatgaag
     3121 ctgggataaa attggaagta ttgaatcctc ttgaaagtct tacaaaagaa caaatggata
     3181 atggtgaaga ttatgtttca gtaatggaag acaatctaaa agcgttagag aaaacaacta
     3241 tggttgctgg tgaagaggta gttccagaaa aagaagcgaa ggatgaaaag acagttgcta
     3301 atgggtactt taaagatgcc gatgtgaagg atcgcgaatt gagtgattat acaggcgaat
     3361 ggcaatctgt ttatccgttg ttaaaagatg gaattttgga tgaagttttc gattacaaag
     3421 caaaactcaa taaagacatg acagctgccg agtacaaaga ttattatact actggctata
     3481 agacagatat tgatactatc aacattaaag acaacaccat tgattttgta gtcaacggtg
     3541 aacatcatca atacacatac aaatatgttg gatacaaaat tttgaattat gaaaaaggaa
     3601 accgtggtgt tcgttttaat tttgaaactg atgatgcagg tgctggtcgt tttaaatata
     3661 ttcaatttag cgatcatgga attgcaccaa gtaaggcaga gcatttccat attttctttg
     3721 gtggagaaag tcaagaaaaa ctttataatg aaatgcataa ttggcctact ttctatccag
     3781 cttcattatc tgagcatgag attgctcaag aaatgatggc ccattaaaat ccaatctttg
     3841 gaggaaatac atggtaagaa aagtattatt gtctatggat tccaagaata ttgatgctta
     3901 tcggcgttat cgatttgcaa agcaaatgag attaaatggg agtacattag ctgatattgc
     3961 tgcaacttta catgttagtg ttgacacagt tcatagagca ttaaaaaaag atccagattg
     4021 ttgttttatc aaggttaata ctggaagaag tagacgttta gcaaaacgat ataatatctt
     4081 gataaactaa ttgtaacact aaagcagttc aattcttcct taatggaatt tgatctgctt
     4141 tttattatgc atagaaagag gtttcctatg aaaaatatgt cacaattcta ctagttattt
     4201 tgatttaaga aaaacaacac ttttaaatca tatagaagag ataattagca gtaaaaatga
     4261 agaataggta atattagaat gggattcaac taacatattt aaaaac
//
LOCUS       DAXWLH010000189         4273 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXWLH010000189 DAXWLH010000000
VERSION     DAXWLH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.195"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            61..915
                     /locus_tag="ACGE36_002497"
     CDS             61..915
                     /locus_tag="ACGE36_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8384847.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1012..3261
                     /locus_tag="ACGE36_002498"
     CDS             1012..3261
                     /locus_tag="ACGE36_002498"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8384848.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3264..>4273
                     /locus_tag="ACGE36_002499"
     CDS             3264..>4273
                     /locus_tag="ACGE36_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8384849.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIG"
BASE COUNT         1507 a          604 c          788 g         1374 t
ORIGIN      
        1 atgtaataat ataaacataa taaaacaaaa attatccaaa tctaacatag gagggtggat
       61 atggaaaatt ctatatattt agtaacagaa cctaacactc agctgaaaga ttgttacttt
      121 aatttttgtg ggtattcaaa aacagaacct agacatagtt ttggaccagc tattcgagaa
      181 caatacttaa tacacatagt gctagaaggt gaaggctatt attcaattaa tgatcaaaaa
      241 tattttctga agaaaggcca aggatttgtt atcccaccag gagtatctac tttttatcaa
      301 gcagatgaaa aaaatccgtg gtcatatgta tggttgggtt taagtggcaa tcttgtagaa
      361 tactatctaa aagaaattgg tgttggaaag aatcatctta cctttaaagt cgaaaattta
      421 aattcattta aaggattaat ttttgaaact ttatcgtatg atcaggacaa tctagtcagt
      481 gaattgtcca gacaaaaaca agtttataaa tttttagaac tcctcttaaa ttcagctagt
      541 tatctgaacg aagggataat tactaaaaag atgaatcctt ttgttcgtca aacaattgaa
      601 ataatacgtg agaatgcgta tcaaaaatta tctgttaaag atattgctgt cagtctatcg
      661 atacacgaaa attatttatc acgattgttt aagaatgata tgggaatatc aataaaagag
      721 tatattagtg aaatcaaaat ttctattgca aacgatctgt tatcatcaac tgaatattca
      781 atccagcaaa tcagtgacaa gataggattc tcaacagtac aatctttttc taaagccttt
      841 aagaaagcaa atggtgtttc accgggagta tatcgaaaac gtagattagg tctaggaatc
      901 attaatggtg aataaactaa acatttgatt ttgataaata ttcgttactt taaacgctta
      961 ctatttatgt gagcgttttt tataatgcaa taaagaaacg gaggagagct tatgtataag
     1021 gataatgaaa tgaatattat ttatcgatca gaaaaccaac aatttcattt gtttaatcag
     1081 gagataagtt acatttttaa gataccggaa gatggtaaat tattgcattt atattatggt
     1141 gagaacttac cagataatga ctatagtcat ctaattgaga tgcatcatcg gcctatgaca
     1201 acctatagaa atgaaaacga tttgctatat tcgttagagc acttaaaaca agaatttcct
     1261 gaatatggta caacagattt tagacatcct gctatttcat tactacagaa aaatggctct
     1321 cgcttatcag attttgtcta tcaagggtac gaagtggaag aaggaaaacc taaactagaa
     1381 ggtttacctg ctacctatgt tgataataat gatgaaagca aaacattgaa agtttatatt
     1441 ttagattcag ttacaaatgt tcaagtagaa ttattatata caatttatag agattatcct
     1501 gttattactc gtagcgcgcg agttatgaat aaaggaaaag atattcaaat aatcgagaat
     1561 attagcagtt tatcattgga tttacccgat gcaaattatg aatggcaaca attatctggt
     1621 gcttggggta gagaaagaac tattaagtcg cgtactttac aacaaggaat tcaatcaatc
     1681 gaatctactc gaggtatttc aagccatcag cataacccat ttgttacttt aaaaagaaaa
     1741 aatactgatg aaaatcaagg agaagctatt ggtgcagcac tagtttactc aggaaacttc
     1801 ttaattcaag cagaagtaga tacatgggat gttactagac tacaagttgg catcaatcca
     1861 tttggattta attggaaact caatccagga gaaatattta cagcaccaga agcgattctg
     1921 gtttattcaa gtaagggttt aaatgccatg agtcaaactt ttcatcaact atttagaaaa
     1981 cgcttagctc gtggtaagtg gcgagaaaaa gaccgtcctg tcttaattaa taattgggaa
     2041 gcaacatatt ttgattttga cgaagaaaag ttggtaaata ttgctaaaaa agcatttgat
     2101 gttggtattg aattatttgt attagatgat ggctggtttg gagaaagaga aaacgattgt
     2161 gcaggattag gagattggca tgtaaatcct aaaagactac ctagaggaat cggcagcctt
     2221 tcagaaaaaa ttcgtagttt tggtatgaaa tttggtttat ggtttgaacc tgaaatggtg
     2281 aataaggata gtgagttata tcgcacacat ccgaattgga ttatttctac acctaatcga
     2341 aaacctaatc atggtaggaa ccaatatgta ttaaattttg ggttagatga ggttgttgaa
     2401 aatattttcg atcaaatgtg taaaatcatt gatgaatcaa acttagatta cattaagtgg
     2461 gatatgaatc gtccattgac tgatgtattt gacagtcact tgtcagcaga tcaacaagga
     2521 gaagtattcc acagatatgt actaggcgta tatcgcttat atgaaaaact tataacaaaa
     2581 tatcctaaca tactatttga gtcttgttca tcaggtggtg ggcgctttga tcctggaatg
     2641 cttcactatg cacctcaagc ttgggctagt gatgattcag atgcaattga aaggttaaaa
     2701 attcaatatg gaactagtat gctgtatcct ctttcgtcta ttggtgctca tgtatcaatt
     2761 gttccaaatc atcaaacaaa ccgtataaca cctataaaaa ctagaggaaa tgtagctttt
     2821 tttggtgcat ttggatatga gcttgactta aatgaattaa gtgaaggcga tttgaggatt
     2881 gtaaaagaac aaattgcctt ttataaaaaa tatagaacca taattcataa tggtactttt
     2941 tatagactat tatcaccatt cgatgatgat aatcaaaccg cgtggatggt tgttagtcat
     3001 gataagaaaa cggctattgt tgctcattat aaaacattaa atgaggtaaa tgctccttac
     3061 cgccgactga aattaaaagg gttagataat gacggacttt ataatgtagg acaaaatagt
     3121 ttgcgatctg gaatggaatt gatgagagca ggactagtct gttcagatgc atctagtgga
     3181 caaataaatg atcagaatcg acgcccagaa acatttgatt ttgattccaa gatttgggtt
     3241 ataacacagg aggaaaagta gagatgaata accaaaaaag cttacttcaa aaaggagcaa
     3301 tcttaagtat ttcactaatg ttaacaagct ctcaagcaat taatggtgtt attccacaaa
     3361 tgaaacagtc tttggggata tcacaaagtc aaagtgaact tttaggaact gcaccaagta
     3421 taactgttat tttgtttatc ttattatcta gttactttgc caaaagaata ggaatgaaaa
     3481 agacaataat tttaggttta ttattagctg gtataggagg aattattcca gtatttacag
     3541 ctaccttcca aaccgttctt attagtcgag taatattagg tgccggttta ggagtatata
     3601 attcattggc tgtgacatat atcagctctt tatactctgg caatactaga gcaacgctat
     3661 taggtattag aaattcaatg gaagctttag gtcagacaat ccttattttt cttgctggag
     3721 ttttagtgaa cattaattgg acggcttcgt ttttagtcta tgcaattgcg tttcctgtag
     3781 ctctcttatt cgcgctaaaa gttcctgaaa ttagagatga aaatagtgac atacctgaaa
     3841 atcatgtaaa agaaaagatg aatccaatgg tttttgccct agttctattt gcaatcttac
     3901 tcgtaatgaa tagtattgct atttctgtac gatttgcatc aattgcaact gaaattaaag
     3961 gggtaaactt taatgctagt aactatttag ctctaatgcc tatcttagga atactagcag
     4021 gatttatgtt tggaccaata aataagtgga taggaaaagg aaccctatac ttgggaatta
     4081 tattctttat aatttcaaat ttgttaattg ctgtgtcaaa cggtaatatg acgttcctgc
     4141 taacgggttt atttttatca agtataacag gatcatggtg cttcccattt atctttagta
     4201 acttagataa agtcactaca aagaatacaa ttaattttgc tacttcattg atttttattg
     4261 gctgtaatat tgg
//
LOCUS       DAXWLH010000190         4213 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXWLH010000190 DAXWLH010000000
VERSION     DAXWLH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.196"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            478..1446
                     /locus_tag="ACGE36_002500"
     CDS             478..1446
                     /locus_tag="ACGE36_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8384850.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1514..2272
                     /locus_tag="ACGE36_002501"
     CDS             1514..2272
                     /locus_tag="ACGE36_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFF8384851.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2340..3050
                     /locus_tag="ACGE36_002502"
     CDS             2340..3050
                     /locus_tag="ACGE36_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8384852.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3086..>4213
                     /locus_tag="ACGE36_002503"
     CDS             3086..>4213
                     /locus_tag="ACGE36_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFF8384853.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1411 a          631 c          892 g         1279 t
ORIGIN      
        1 ataaattgta ttaaaatcaa tgtatcactt tttaatcagt tttaagacca taaattgcgt
       61 actaaaagaa attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc
      121 acccatctac ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg
      181 tgcaaaaacg atttcttcta aacaaatcaa gacaaactta taaaccttca tcccagcacc
      241 ataattattt aaatcatagg catttttcga aattagtata tgtactcaag tttcactgca
      301 gattatttat attttttaga gaaataaatt tttcctattg acaaacacaa agaaagcgga
      361 tacaatgcaa ttatgtcaca tgacgttata tgacgttatg tgatatcttt acatttaatc
      421 agttactttt attttggata aaattaacat ttttaaaatt ttaaggagga aatgttgatg
      481 gatgtagcaa aaacggttaa cgcaattgta gaaaaaaatg atggagagat ttctggaatt
      541 tatttggtgg cttgtggcgg ttcgctggta gatatgtatg tctcagatta ttttcttaaa
      601 gccgaagcat cgaagtgtat tacaggctta tatacagcga acgaatttaa ccatgtaacc
      661 ccaaagcaat tagatgataa atctgttgtc attctatgtt ctcatggagg aaatacagca
      721 gaaacggtaa aagcaggaga agtcgctcaa gctagaggag ctgtgactgt tggtctgact
      781 cataataaaa ctgcggattt attaaaggtt tctgatcata gtttccttta cgaatgggga
      841 gatgatgcaa aaatagtcaa taatcctatg gccatcattc ttgatttaac tgtatctttg
      901 gtcaatgtta ccgaaacatt tgaacattac ggtaaattca gagaaggcat gaaggttatc
      961 gataccgtaa ttgaaaatgg cattaagcag gtgcaggatc gtattaaagt gtttgctgat
     1021 aagtacaaag atgaaacaat gttttatatc ttaggtagtg gaccatcata tggacatgct
     1081 tatggttttt caatttgctc tttgatggaa atgcaatggc ttgattcaac ttctgttcat
     1141 tctggagaat tcttccatgg accttttgaa gtaacagaca aagatacgaa ttttattctc
     1201 ttgatgagca ttggacgcac tcgtgcttta gatgaacggg caaaacgatt ccttgataaa
     1261 tatgcagaaa gagtagaaat cgttgatgca aaggaactag gattggatat gatatcagat
     1321 gaagtttctg aattcttcaa tcccatttta ttctacagta ttctttcaga atatcgttca
     1381 gctttagcag atgtacgcaa tcatgattta gatgttcgtc gctatatggg gaaagtggag
     1441 tactaggagg attaaaatga aggtattagg cttaggtgat aacgtcgtcg ataaatatga
     1501 aaatctggga attatgtatc ctggaggaaa ttcattgaat tttgcagttt ttgcaaaaaa
     1561 actgggacat gaaagtagct ttatgggagt ttttggctca gatgaggagg cagctcatgt
     1621 tttatcagca ataaaagaaa taggactgga caacagtcac tctcgctttg aaaaaggcga
     1681 gaatggctgt gccagagtga agattgatga gggcgaccgg attttccttg gcagcaattc
     1741 gggaggtgtt actcgtgaat acccgattca gcttactgaa gaagacagag aatatttaaa
     1801 ccagtttgaa ttgattcata tgggattata tagccatgtc aatcatctat tagaagagtt
     1861 acaaaatgtt tctggaaaga tttcttatga tttttctgac gactttacgg aagaagaggt
     1921 tcagcgggca attgataaag ttgattttgg cttcttttct atagaagact tttccgatga
     1981 agaagtaaag cagtttttga tgaagcattt ttgtgagcga aatgatgtat tgattgcaac
     2041 ccgaggagaa aaaacggcta ttggttatga tggagcaaag ttttatgaag tgaaccccgt
     2101 gctagaagaa ccagttgata caatggcagc gggagattct ttcttaacag ctttcctact
     2161 ttattttctc gaaggggacg atatagtcac cgcgatgaaa aaggggaatg aatttgctgg
     2221 gaagtcttgt ctggttgacg gttcgttcgg ctttggtatt cattacgatt gagcttgttt
     2281 taaattcctg catttgataa cctaaagaaa gaaatcccgg taaagtggag gttaaaaaaa
     2341 tgctggatac aaatgcacaa ttaccattgt acttacaatt aaaagaaata ttaaaaaata
     2401 aaatcaatac aggggagtat cccgagaata cgaaaattcc cgctgaatca gagctgatag
     2461 aaagttatga ggtcagccga atcacagtga gaaaagcagt agaagaactg gtagaagaag
     2521 ggtacctact taaaaaacaa gggaaaggaa cttttgtcaa ccggcataaa gtttttcgta
     2581 aaattgaata tattatgggt ttcagtgaga gttgtaaatc taatggtttt acaccgacta
     2641 gcattgtttt ggaacgcaag atcattccag caactgaagg gatggcaaaa aaactcgata
     2701 tcgctcaagg tgatgatgtt ttatatatcc agagaaagcg aatggcggat aacatcccca
     2761 ttatgttaga aaacaattat ttttcttatg aaagatatag tttcttaatg gaggaagagc
     2821 tgactgattc tttgtatgaa gtacttaaag tccaaggtac cttagccatc aatcctgggg
     2881 aaactgcact tgaaattgtc acagctgatg accaaattgc caaaataatg gaagtagctg
     2941 ttgggacgcc ctttttcttt atggatactt taatcaacga tcaaaacaac ataccaattc
     3001 atgtaggtaa tcaatattac ctgggagaag tatatcgttt ttacttatga tttcctgttt
     3061 ggatttcaag gagtagattt tatttatgaa gcaaatattg cggtcaaaaa tgatttttga
     3121 tggagagcag ccacctttca aaggttatat tgtaatcaat gaagggatca ttgaaaaggt
     3181 ggaaaacaac tggaattaca gtgatctatt aaatgaaaac acggaattaa ttgcttatga
     3241 tgatcaattc gttatgccag caattcatga taatcatgta tttttcagtg gttatttggc
     3301 tatgaatgct ggcatagatt taagcgatac atcttcagct aacgaggctg tagagcgtat
     3361 taaagctgta atgaaccata aaaaagcaga tgaaccaatt tatgctcatg gctgggatgc
     3421 tgccctgtgg aaaactgacc catcagaaga gctattgaat gaaataaacc actttggtcc
     3481 aatcacagcg atcgacaaaa atcgcagcca ttgttggatg aatgctattg ctaagcagac
     3541 ttatggtttt acagaaaaag aaaccagtgc tgaaagtcga gtattattaa ttaaggaaat
     3601 gctagcaaac aaaaagcttt tagcagaggt ttacgctgaa tttgaagcat tactgctttc
     3661 aaaaggtgtt atatcaatga aagaaatcgt ttttgatgac gcagaatttc tttctaatat
     3721 acctcagagg aaaatactta gtaacctata tgtgcaggca gtccaagaac ctctacagca
     3781 tgaacttcta tatgagtata aggaaaaagt tttccctgaa aaggtccgct ttggtggagt
     3841 gaagatcatg gtagatggtg ttgttgcgga tagaagtgga gatatttacg gtgtttatca
     3901 gtcaggagta gcttcacccg agattgacta tgaggcaatt aaagcagaag ttgctgcatt
     3961 caatgaagta gagattcctt gctgtttgac aacggaagga gataaagcgg gttataaagc
     4021 tgcgtcaatt cttgtcgaat ttggaaagcg gttacctgaa ggcgttttta attcgattag
     4081 tgatttggaa atggttactc aaaagacagc agaaaaaatg aacgaaggac aagtagtagc
     4141 agaggtatat ccacaaattc tcggcttgaa tcaaacgtta gaagaggctt acatgtcaac
     4201 tgttataaaa aat
//
LOCUS       DAXWLH010000191         4200 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXWLH010000191 DAXWLH010000000
VERSION     DAXWLH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.197"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            53..1477
                     /locus_tag="ACGE36_002504"
     CDS             53..1477
                     /locus_tag="ACGE36_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFF8384854.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1658..2431)
                     /locus_tag="ACGE36_002505"
     CDS             complement(1658..2431)
                     /locus_tag="ACGE36_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8384855.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2428..3333)
                     /locus_tag="ACGE36_002506"
     CDS             complement(2428..3333)
                     /locus_tag="ACGE36_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384856.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3487..3807)
                     /locus_tag="ACGE36_002507"
     CDS             complement(3487..3807)
                     /locus_tag="ACGE36_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFF8384857.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1281 a          764 c          831 g         1324 t
ORIGIN      
        1 aaggtttgtt atactttttg ataaaagcgg ttttattttg gaggatgctt acatggatac
       61 agagcaacag aaaaaaggaa ttggtttaat tccacttgct gccttagtaa ttggttcagc
      121 gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg gcggcccagc
      181 tattttcagt tgggtactcg taggaacagc aatgttgatg ctctccttgt cgatcaataa
      241 tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag aagaaggttt
      301 tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct ggttaggcaa
      361 cgtagctttt gcaacattgt tgatgagcgc attaggatat ttcttcccag tatttgaagg
      421 tgggcaaaat ctggcatcga tcgtgatgag tacagtcttt ttatggggat atgcatggct
      481 agtgaatcgg ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat tctgtaaatt
      541 agtgccatta tttttattta ttgtagtagc tatcagcacg ttttcgatcc aacaattcat
      601 tgataacttt tctagcgaca ttatattgaa tgcgaatgga cagccgatcc cgttttccag
      661 tcaagtattg agttgcatca tggtgatgct ttgggtattt gttggtattg aaggagcctc
      721 tgtcgtaggt tcacgagcaa aaaaacaatc agatgttggg aaagcaacga ttctagggat
      781 attcggcttg attattattt acgtcttggt ttccatgctt ccatatggga caatgacgat
      841 gaatcagctg caaacgatca aggcgcagcc tgctatgggc tatgtgtttg aaatgatggt
      901 tggtaaatgg ggagcagtcg tgatcaacgg cggtttgatc atttctttag ttggtgtttg
      961 gctctcatgg acgatcttac cgattgaaac aatgggaaac atggctgaag acggactttt
     1021 gcctaaagca tggggaaaac tcaacaaaaa tggggcacca acttatgcta tcgtattgac
     1081 gacgttatgt acgaatgtct ttttactttc attattattc actgacagtg cttataactt
     1141 tgcttacaca cttggtacag cagctatttt cttcacgtgg cttttcttag gattgtacca
     1201 aatgaaactt tcttatcagc ggaaagaatg ggtccaattt ttagtagggc ttttagcaag
     1261 tgcctttcaa gtatgggcta tgctagtcgt agcctttaat gaagtaatgc ttgttcttgt
     1321 tcttttcttg ccaggcattt atttttacct caaagcacgt aaggagcaaa acgagcctgt
     1381 acaacatatc cacttagata agattttact tataggtgtg acgatggtcg gagtgattgc
     1441 aatggtttta tttgccactg ggatgcttca cgtatagaag tataaaagaa gtataaagag
     1501 aggttgtgag cctgtcgttt attcaaaaac atactaggaa aggagctgtg acacaaatgt
     1561 tgtcacagct cctttttcag aataaacggt gtttaagaga cagccaatcg gcaataaggc
     1621 tgaacgtctc tttcacagcc tcttttttga tgagtcatca tacatttttt cgcttaaata
     1681 cgagatatcc taatgctaag aacaaaacga tgtaagctaa attaccgaat agcatttgat
     1741 tagtagaaag atgagtcaat tgcttcatga tctcttcgtt tccaatttgg ttttgcagat
     1801 tcaacatact gatcggattc catttgatcc actcccattt ttggatgagt gcaataagga
     1861 taccagatat gattgacgaa gcaaaataaa agacgattcc tgctgagatg gatgcgccgg
     1921 aactattgat aaagcaagca atcatcaaca ctaaacttaa aatcagccat aaaccgatat
     1981 aactacccaa agtataagtg aacaagcttt ggatcagtgt ttgaccagta gaaaccttct
     2041 ccgtgaagga aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca ataatagcta
     2101 aaaggtataa ataaacagaa taaataacta atgtgatcca tttgcttact aatatttctc
     2161 ctcgagaata ctttcgataa aggagatttt tcaatgtacc atattgtgct tccatcgaaa
     2221 tgatcgtgct tgcagctgca atcatgataa agacaatcca agatgtggca ctaaaaagct
     2281 gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt tttggctaga agtgctgttc
     2341 ctatgagcaa aacaactaat agaacagata aaacagcagt tgactttttc ttgatcaatt
     2401 taaataattc ttgttttaca agcaatgtca tttttcttct tcccccttta ctgaatccag
     2461 aatttccaat agagaagatt ccaaatcatt cgtcagatgc tggacgtctg tcacatggat
     2521 attttgtgca gacaatagct ggatcacggt tcctaataat tcttcgtttt cttttaaaag
     2581 cttgatttta tcaggatctt ccaaagtata tccagcttct gtcaagatcg ttcgagcagc
     2641 tagatcattt gttgtagaga taacgatgaa atcttgcgct gtttggttca attcagccat
     2701 tgtacattgt ttgatgatct ttccttgatt gatcaggatc agctcatcga ccattttttc
     2761 aagctcgctg agtaagtgac tggagatcaa gaaagaggtg ccctgagtag ccaagtcggt
     2821 aatgattttt cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg gctcatctaa
     2881 aataacgata gacggatggt tcagtaaggc aagagcaatt cccaattttt gtttcattcc
     2941 caacgagtag gatttcgctt ttttgtgaat gtacttttcc atcttgagct gattcacgat
     3001 agtgtcgata gcagatacat cgttggtttc agagaataac ttcagatgat catagcctgt
     3061 taagaatgga tagatacccg ggtattcgat caatgctcct acttgttcca accctttatg
     3121 actagaggga gaaatctcat tttcaccaat acagattgtt ccatctgagt aatggatcag
     3181 tcctaaaata gctttcatga tcgttgtttt tccagcgcca ttaggaccta ctaatccaac
     3241 gattttgcca ggggataagc taaaactgac atcgctgagg gcttgtttgt gaccaaagtt
     3301 cttggataat tcttggaccg aaagactttt caaaaatatt cactcctttt ctatagatag
     3361 tacaagcata cacctgatga gtggagaagt cgctaggtca ttggacttat ttcttatttt
     3421 ttcataaaaa aattgaatat taaataaaaa ggcttatttt ttctgatcag tacttattcc
     3481 aagtgtctag tggccgctag taatcttgat gccaatgata ctgatgagta gcatccccac
     3541 aaagaaccaa gtggaaagat tcagcttatc atgaaacaag accacaccaa ttatgaccga
     3601 accaactgcg ccaatccctg tccagatagg ataagagaga ctcatcggca aacttttgat
     3661 tgcctttgac aaaaagtaaa aactagcaat catgccaatc actgtgtaaa aagaataatt
     3721 gattttagaa aaaccatcac tccatttcat catcgttgac cagaatactt ccaaaattcc
     3781 tgctatgatt aattccatcc atgccatact tacattcctc cttaactata aaaaaataca
     3841 caaaaagagg aaaatcatcc cttcaaagac ctaatggaat gatcatcctc tttatcctac
     3901 ccggtggcag attgtatatc tttttctgta gtatagcaca cctatccaga tatcggcaat
     3961 ctcatacttt ttagtaaagt gtaccaagca ctgacggata tatcgttagc ggaaaagaag
     4021 atgaatgctt tgcaatttca tagaaagaag ttgttattta ttaatcattt aaaaaaaaca
     4081 gtaactagac cttatttcta tatattatcg ggcaataaat taaaaaaagc tttaccttac
     4141 aataagtaag ataatttttg tgataaaaaa ataacgaaaa atatttaaaa atagtgtcta
//
LOCUS       DAXWLH010000192         4182 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXWLH010000192 DAXWLH010000000
VERSION     DAXWLH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.198"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            66..305
                     /locus_tag="ACGE36_002508"
     CDS             66..305
                     /locus_tag="ACGE36_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384858.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(667..927)
                     /locus_tag="ACGE36_002509"
     CDS             complement(667..927)
                     /locus_tag="ACGE36_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384859.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1112..1609)
                     /locus_tag="ACGE36_002510"
     CDS             complement(1112..1609)
                     /locus_tag="ACGE36_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFF8384860.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1739..2449)
                     /gene="budA"
                     /locus_tag="ACGE36_002511"
     CDS             complement(1739..2449)
                     /gene="budA"
                     /locus_tag="ACGE36_002511"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFF8384861.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2462..4126)
                     /gene="alsS"
                     /locus_tag="ACGE36_002512"
     CDS             complement(2462..4126)
                     /gene="alsS"
                     /locus_tag="ACGE36_002512"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFF8384862.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1201 a          888 c          663 g         1430 t
ORIGIN      
        1 cattgatttt gcctaatact gagcataggt gtatgctaaa cgatactata aggagtgtgt
       61 acataatgaa aagaattctg tttatcagtt tatcaaattt attgttactt actgcttgtg
      121 ggacttctgc atcaaagaac ataccttcag atacgcaaat ttcgaatagt atcgattcaa
      181 caatttctac aaatgagaaa aattctccgg tagaaacttc cgatataaaa gtttctacct
      241 ctgcaaaaaa cagggtaaag aaactgtcag cacgtctcct ttatttcaaa caaactctga
      301 attagcttct tcatcttctt cagcaaataa tgatgatagt ttcgcgttta ctcctaacta
      361 aatgcaacct ttacaaatgc tttacgataa caagcctgag ctaaatgatc ctgatatcag
      421 ttttgcattt tttgaaatga ttggatcgga tgatcttttt aaaactacta gtttgtctat
      481 ccgaaaatag gacagttctg gcagtatagg tttttatcga gtttctccac caggcctcgt
      541 tacagagaca gattctctag gcgaaccttt tgaataaatc aaaaatcatg aacaatcact
      601 gccaactaaa taaagtgcct tcaaaagctt ggtcttctcg cttttgaagg cactttattt
      661 aaatctttat gaaactaaag tatgttcata tttgttgcta tagttatata gatattctgt
      721 tgtttccaac tcataaacgt gagtatattt tgccactact tttccgcatt tttctaatat
      781 ttcattgcga tcgacgtcat gatattcgtc tacgcagatc acaacacaat tttctaattt
      841 actgattact tctccaatca ctttttgttt caagccaata ggttggcaaa tatatttttt
      901 tcctaaaatg atagtttcgt tattcatatt tcatccatcc ttaatgtttt ttcattattt
      961 aaaatgataa taacattaaa atatgattat gaaaatcaat aacacttgtg attttataaa
     1021 aaaataaaaa aaccgaaaaa cagaaatgcc cactgtcttt ccggctcttt ttattcttga
     1081 tttctcttct tttttgtttc aatagacgat tttagttatt aataaatgaa aaagcattta
     1141 tattaaaatt tgctaataaa agatctacat atttttctag aaatgcttga tcgatttcat
     1201 catacccttt cgttactccg caatcgatat ctaaaacacc gattaaatta ccttctttta
     1261 ccattggcac tacgatttct gattgtgcgg ctgaatcgca ggaaatgtaa tttgcatggg
     1321 ttttgacatc atcaacgatc aatgtttctt gtttttcagc tgattctcca caaactcctt
     1381 ttcccatctt gatacgtgtg caagaaactc gcccttggaa cggtcctaaa atcaattcat
     1441 taccatcgaa taaataatat cctgcaaaga cagtctcagg caaagtctca ttcaacaatg
     1501 cagatgaatt tgctaaattt gctaaccagt tatcttctgc ttctaataat ccttgttgct
     1561 gtaataataa aatttcgtat gcatcttttt tctgtttttc tgtccacatg ataaaaaccg
     1621 ctcctttttg atctatggag aagattatag ctcagttttt ttgtaaacgc taggttttct
     1681 cctcaaaaaa acgagatacg tcaggttaat caccttgaac gcatctcgct gtctttttct
     1741 attctgcttg ttggatttct gctgctaaat cagcataatc aatcgtttcg tgaacaaaag
     1801 tcggatcatc aactggaaaa tgctgtacaa gggacgagat gcggctaatc tcaacttttc
     1861 cttcagccac ttcaaaatcc attacatgtc cgccaaatgt atgtgtatca tcgataaaat
     1921 gcaggtgaaa tcccgctgca gcaactcctt ggaataattc aggcgtaaag aacccgacta
     1981 atgttccttg gacattgctt tgttggaatt ctggttgaga ctctgaaatt tttgctagtc
     2041 ttctgtaagg tttttcttgc ttcggcatga tacggatgtg catatttttg aataatccag
     2101 ttatcttcac tgccaaaaat aggttttccc cggcttctct tactaatatg tcttttttca
     2161 attgttctcc atctgcagtt tttgagacag aaaaactagt atctggcgta aaatctgtaa
     2221 ttgctgcata gggggtaagt tcacttccat ctaattgggc aactgagcca tctgatcttc
     2281 cttgataagc tacgccatct aaaaagatga cttcaccatc aagcccatgc aatgttccca
     2341 atcccagagt tcctttttct aatatatgag caatcgtttc tgttccatcc atcaagccgg
     2401 ccatcaaagc tcctaatgtc ccgtgctggt ataagatttt ttcagtcatg tcgacacttc
     2461 cttaatagaa ttgatctggt aagattgttt ctcccaattt ctcattatca cggtaatcaa
     2521 tcggaatatc aataattacg ggtccttctg tcgcaaatcc ttcttctaaa gctttttcta
     2581 attcctctac acttgtagct cggattcctt tagcgccaaa agcttctgca tatttcacaa
     2641 aatcaactgg accaaagtct acacctgacg cccgctgata tttcattttt tcttggaact
     2701 ctaccatatt gtagtgtcca tcattccaaa tcaaatgaac gatattcaat tttttacgta
     2761 ccgccgtttc taaatcctgg gcagagaata aaaatccccc atcgccggaa actgatacga
     2821 tctgtgtatt tggacgcacc aatgcagcag aaatcgccca tggcaatgca acaccaagcg
     2881 tttgcatacc attactgaat aacaaatgac gaggttcata acttctaaag tggcgtgcca
     2941 tccaaatata atgactacct acatctacgg taacagtcat atcatctgtt acttttgact
     3001 gaagcgtgtt aataacttct aaaggatgca agatacctgc ttctcctttt gattgaacaa
     3061 tgtcccgctc cgttaatttt gcttgaagag atgctaaata ttcttttgca tcttctgaca
     3121 cttgttgagg ctctaatgaa cctgtcaata aatcaagagt ggctgagata tcaccgatca
     3181 attctctttc aggctgcata aaaggatcga tttcagctgg cgcttcatcg atcacaatga
     3241 tccgcgcatc tttttccgca ttccagtttc ttgcttcata ttcgattgga tcatagccaa
     3301 ttgcaataac tagatcactt cgtttcaaaa gcatatctcc tggttggttt ctaaataaac
     3361 ccacacgacc aaagaaatgc gcttctaatt cacgtgagat cactccagct gcttggaatg
     3421 tctctacaac aggcaatgct gtcttttcaa ctaattgcct gatggctaac gtttcttttt
     3481 cacttgatcc tcgcatacct actaatagta ccggtagttt tgcttctcta atgcgttcag
     3541 ctaggtaacg gatatcatct gcagaagcag aacctaattt aggatcgctc aatggcttga
     3601 tgacattacc ttggacaggt gcatccacta catcttgcgg gatactgata aagctcgcgc
     3661 ctttttttga actcttagcc atacgataag cattggcaat cacttctgaa attgtttctg
     3721 gatcttggat ttctgcactg tattttgtga ttggctggaa cagtgctgca ttatccatgc
     3781 tttgatgggt caacttcaat agatcgcttc gttttacttg tccggcaatt gctaaaactg
     3841 ggtcaccttc tgctgtcgct gttaccaaac ctgtagctaa gttactagca ccaggaccac
     3901 ttgttgcgat aactactcct ggctctcctg taatacgtcc gattgcttgt gccataaatg
     3961 cagcattttg ttcgtgacga gtcacgatta attcaggacc ttgatcctct aattcattaa
     4021 atacaccatc gatttttgca cctggtatac cgaatacata atcaacatga tgattaatca
     4081 aactttctac tacagatttc gaaccttgtt tcattttttc agccattgaa catcgtcctt
     4141 tttatgcttt atttttaatt attagttaga aataatcaca aa
//
LOCUS       DAXWLH010000193         4111 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXWLH010000193 DAXWLH010000000
VERSION     DAXWLH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.199"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(814..1767)
                     /gene="menA"
                     /locus_tag="ACGE36_002513"
     CDS             complement(814..1767)
                     /gene="menA"
                     /locus_tag="ACGE36_002513"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFF8384863.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1780..2883)
                     /locus_tag="ACGE36_002514"
     CDS             complement(1780..2883)
                     /locus_tag="ACGE36_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8384864.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2973..3908)
                     /gene="pplA"
                     /locus_tag="ACGE36_002515"
     CDS             complement(2973..3908)
                     /gene="pplA"
                     /locus_tag="ACGE36_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFF8384865.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT         1214 a          818 c          724 g         1355 t
ORIGIN      
        1 tcaaaacgaa ataagtaggg aagttggaga ttttgattat cttcgtaact gacgaagagt
       61 aaatgaatcc atttggtatg cactcttaag ttcaaaatat tggtatcgat gtttaaaaga
      121 aatagaaatg tatcgaagaa cagatgttta aaaaagtgtg actttgtata aataaattag
      181 aagaagcaaa cgaggatgtt aaaagaatac catatagtta ccaaaccaag aaagttctcg
      241 tcaagttcgc tggtacgaat caaatcaagt gaaaaaaggg actgtgaaaa aaattgtgtc
      301 atagttagtc actttttccg aacaaatagt gttcaatcag ctgtccttcg gtattagctc
      361 aaaaaacggc aaaatatgag gaactatttt tccatttgtt tctctaatac tcaggacaaa
      421 acactgattt tacaacctct gataaacggt gttcaaaagc cagtttctcg gtattaagcc
      481 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      541 agcgactttt tcacaacctc tgataaacgg tgttcaaaag ccagtttctc ggtattaagc
      601 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat actcgaaact
      661 aagcgacttt ttcacaacct cctgataaac ggtgttcaaa agcttgcatc tgcggtaata
      721 agcccaacca aacgaaaaat ataaggagct atttttattt ggctgttctt attacttgat
      781 gcagggcagt tttttcacaa cctcttcatg ctgttaaatg gcttgcataa tgattgttag
      841 taatagcccc aatacataac tggcattgaa taagaccata tttttgatgg aatagctgaa
      901 actgataggc tggggtaacg tttgcttgaa ttgatccaaa tgcttcttca caattggcaa
      961 ggtcaagaag gtgaccaaga tcggccattc atagataccg ctcaacaacc ccattaaaat
     1021 cacgaaatag caagcataca tcaatagctg gaataagact gttcccgcag gacgtccgat
     1081 ataaaaaact agtgtatagc gatgattttc aatatcacgg tcaagatccc gcaaattatt
     1141 agccaacatg atattggcaa tcgtaaacac caagggtaag gaagctaata cgattgctgc
     1201 tatagcccaa agatttccta ccaaatcaaa ttgtccagat gaaaagttga ctgtcagatc
     1261 atagacatct gaaccgaatg tattgacata aacgaccatg gcaaaaattc caaggcccat
     1321 agtcacccca ctgaaaattt cccctaaagg cattcgagat aaaggaatcg gtccgaatgt
     1381 atagaatact ccaataaaac aacagataaa tcccatcaaa agtaaaagcc aacccgtatt
     1441 gatcgttaga ataacgccga taacaaaaac aaatgcaatc atagctaaaa tcatgtttcg
     1501 aatcaataca ggagatagat ttctgcggcc aatgacattt tcttcatact tatatacttc
     1561 agaatgagcc ttttcataat ccatgtaatt attgatagcg gtagtcgcta aatcaaaaac
     1621 tagcattcca ataaaaaata aaaaagtgtt tagccaatgt atctcatgaa aaaaagcgat
     1681 agagaataat acccctaccg caaaaggaaa tacactagcc acctttgttc gtatttcaac
     1741 tacttctaaa aagattttta gtgtcatggt tcgtactcct tattttagtt cagaacggtc
     1801 acccatcgtg taatgggagt cctccgataa tttgaaggtg tctttgattg gatcggtcac
     1861 atatacttta tcgtcttttg tcacgaagat cgcttccgtc ccgttattca attcgctttc
     1921 gacgtattcc agtccttttt tcacacccat tgaaaagact gctgtagaca acccgtcacc
     1981 atcaattgat ttatctgtta tgatcgtgac acttgcgata tcattgtcga acggataacc
     2041 cgtttttgga tcaaacaaat gatggtattt tttgccatct acttctaaat aacgctcgta
     2101 aattccagag gtaactaatg ttttgttccg ttctttaata gaacctaaaa cagatccgcg
     2161 agccatgttc ggatcttgga tgccgactgt ccagtcttgg ttttcgcctc ttgggctgtg
     2221 gcctaagaca tagacattac cgcccaagtc aacgatggca gtggtcactc cttgtttttt
     2281 caagactttc actacttcgt ccgtaatgta acctttggcg attgcaccta aatcgatgat
     2341 cattcctttt tcttttagat aaacagtttt ttctttgtca tttagttcga tcttatgata
     2401 atccactaat ttcaatgcct gatcgatttc ttcttgggaa ggttttctgg cgtcatcaaa
     2461 accgattcgc cataattgtg tgattgcacc aatcgccata tcgaatccgc cttgagaatc
     2521 ttggctatat tcgtaggccc gcttgactaa cgtgtagacg tcatcagaaa cttttactgg
     2581 tttgatccca gcttgctcat tcacttcatc gatctcagaa cctttttgat tgatcgtgat
     2641 tttatcacca agttctttta ctcgatcgaa ggcaggtttc aatgctgatt ctttgccttc
     2701 atcatacaca cggatacgaa cgtaagtccc taacaagaac tgttcatcag aatatggatc
     2761 tttcaatatc ttggtagctg ctgaactttc agtggttgtt ttatttgctg agttgctgca
     2821 gccagttagc accataaaag agatgcccag tgtgattaag gaaaatagta gttttttccg
     2881 catctttttc ctctcttcat tttgaaaggt gagacgactg cctaatctgg ctatcgtccc
     2941 accctccaac ttttattagt atgattatgt gattattttt ctgttacgat attatctact
     3001 tcgattgttt gagtgtcgcc tttttcagct gcattcacta attgttgtgc gtacatgatg
     3061 aatgaatgtg agctgtgtgt agcacctgta acaacttcta cattagcagg tgagccatct
     3121 tcttcgccca ttgctttgac gaattcgtcg ttcagagtag ggatgtaagt ttttggttct
     3181 gtaccgcttt ttgctttcat attttcatta tattctgtat catcttgttt tgatttaccg
     3241 tctttgtcga tataatcaaa gtttgattca gttactttgc cgccagcaac tgtcatttcg
     3301 aattgcacac ggtagccgtt agagtagttc ttttcttcaa gcttgtaagt gccgtctttc
     3361 aaatcagcac cattgtcgat ttcgattgta tctgtgttac cagcttgagc agcttggatc
     3421 aattgttgtg cgtagttttg gaagctttca gatgagtgag tagctccagt tacgacttcc
     3481 acaccgcttg cgctttgtgc ttttaagaat tggtcattca actcaggaat gtatttttct
     3541 gggtttgtgc cagattttgc ttccatgttt ttgttgtatt ctgcatcttt tgtttttgat
     3601 tcgccgtttt cattgacgtt atcatatttt gattcagtga ttttgccgtc ttttacaacc
     3661 atttcaaaga ctgcacggta accatttgaa taattttttt cttctaattt atatgtgccg
     3721 tcttgcaaat ctccgccagc tacaactttt gcagatgatt ctgttgtgct ttttgcagtc
     3781 gaagattgtg ctgtagatga ttcagaagaa gagtttgagc ttccgttgtc atctgcgccg
     3841 caagcgccta acactagagc tgaaaatgct aatacggcaa atccagttac aaaacgtttt
     3901 ttcttcattt taatacccca ccattccatt tctagtccac aatttttctt tgtgattcat
     3961 tatacattct aatcttttta gcctataagt caactatttt tgataaataa cccatgtaat
     4021 atttcaaacg ttttctttca gcaaatttcg tgaaaaaacg ctttatttta aaatttgaaa
     4081 aaaatccaaa aggagttaga caaaagaaca g
//
LOCUS       DAXWLH010000194         4040 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXWLH010000194 DAXWLH010000000
VERSION     DAXWLH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.200"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(49..591)
                     /locus_tag="ACGE36_002516"
     CDS             complement(49..591)
                     /locus_tag="ACGE36_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFF8384866.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            726..1115
                     /locus_tag="ACGE36_002517"
     CDS             726..1115
                     /locus_tag="ACGE36_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384867.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1119..1883
                     /locus_tag="ACGE36_002518"
     CDS             1119..1883
                     /locus_tag="ACGE36_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8384868.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2012..2959
                     /locus_tag="ACGE36_002519"
     CDS             2012..2959
                     /locus_tag="ACGE36_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8384869.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3089..3829
                     /locus_tag="ACGE36_002520"
     CDS             3089..3829
                     /locus_tag="ACGE36_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384870.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1343 a          685 c          814 g         1198 t
ORIGIN      
        1 aacatttaaa atttaattta gatattagac agtttttaag tttgtgagtt attttccttg
       61 tcgttttctc acttttttag tccagaaacc acctgaaata tattttggct cataagaaat
      121 gataaaagca cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct
      181 aggagaaagt atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt
      241 cacaccataa ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc
      301 tggcaaaatc gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc
      361 gacactgata cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa
      421 acgatctaaa acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag
      481 tggagcaatc agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat
      541 ataagcaaaa ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac
      601 tcctttcatt tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact
      661 ttatacatac aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga
      721 ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca
      781 gtatatactt aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc
      841 agatgtcgct gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg
      901 gtaatatttt cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc
      961 tagcattagg agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg
     1021 catttatatt atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat
     1081 ggcgatcagg aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac
     1141 gttctgtcca tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta
     1201 ttcccgttgt catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag
     1261 gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg
     1321 agcgcaaaca gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa
     1381 atctgtttga atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc
     1441 agcttgcagt tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac
     1501 atcctcatat agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa
     1561 aagaaatcgt aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt
     1621 tgatttcatg ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc
     1681 ctctgttctt ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat
     1741 ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa
     1801 cacagcgaca tatggtacaa ataccactaa tggaagaagc ttccactttt ttagcaaaaa
     1861 atttgataaa aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt
     1921 ccgtttttta ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta
     1981 agaatgacaa catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg
     2041 tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga
     2101 cgaattgaaa gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc
     2161 tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat
     2221 cgacacaact gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa
     2281 agcgcattat tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg
     2341 atacatcatc acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc
     2401 agttgtcttg tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata
     2461 tggtacagca aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat
     2521 cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat
     2581 gaaggaagtt gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga
     2641 aagatatatg acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc
     2701 agatcggcta gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga
     2761 agaatttggt gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt
     2821 gacaacgatc aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa
     2881 aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat
     2941 cagagagaca acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt
     3001 ttcttaaaat aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa
     3061 agttccattt ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt
     3121 tcctgcgtac gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt
     3181 tgacggacaa gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga
     3241 atttcatttc acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg
     3301 attgaagtca gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta
     3361 taccggccat taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc
     3421 ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc
     3481 atctgttgac acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc
     3541 gattatccca gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa
     3601 aaaaacggat gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag
     3661 aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca
     3721 gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt
     3781 gatcgatgat caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa
     3841 atttatattc ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta
     3901 tccgaaagtt tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa
     3961 taagtaagaa aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa
     4021 atagtttatt tttttataaa
//
LOCUS       DAXWLH010000195         4018 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXWLH010000195 DAXWLH010000000
VERSION     DAXWLH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.201"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            47..1348
                     /locus_tag="ACGE36_002521"
     CDS             47..1348
                     /locus_tag="ACGE36_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8384871.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="ACGE36_002522"
     CDS             1375..1635
                     /locus_tag="ACGE36_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8384872.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="ACGE36_002523"
     CDS             complement(2634..3869)
                     /locus_tag="ACGE36_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8384873.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1138 a          651 c          846 g         1383 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatacatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagtt
//
LOCUS       DAXWLH010000196         4007 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXWLH010000196 DAXWLH010000000
VERSION     DAXWLH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.202"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            57..509
                     /locus_tag="ACGE36_002524"
     CDS             57..509
                     /locus_tag="ACGE36_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384874.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            523..1212
                     /locus_tag="ACGE36_002525"
     CDS             523..1212
                     /locus_tag="ACGE36_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384875.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1337..3688
                     /locus_tag="ACGE36_002526"
     CDS             1337..3688
                     /locus_tag="ACGE36_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFF8384876.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3768..>4007
                     /locus_tag="ACGE36_002527"
     CDS             3768..>4007
                     /locus_tag="ACGE36_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384877.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKL"
BASE COUNT         1383 a          513 c          820 g         1291 t
ORIGIN      
        1 aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg agaatcgtga
       61 aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc tattttcttt
      121 tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta gctgaacata
      181 ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa gaattgtata
      241 aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga attagtgact
      301 cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc aaatccgaat
      361 tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat tccaacgcta
      421 tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta aagcagataa
      481 aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa gatttttggt
      541 gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc tgctgagaaa
      601 gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt tgaagaagaa
      661 gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa aattgtatct
      721 atggataatc cagatataat tcaaggttca tataattcag aaactgatcc tgcttcagtt
      781 tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat tgaaactact
      841 ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca ccttcttgta
      901 ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg gcgttttgga
      961 ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa atcctacatt
     1021 gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg ttctattcaa
     1081 ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg gcctggttgg
     1141 acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta ttatgttgtt
     1201 caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt atttttagtt
     1261 gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat gttcggcaag
     1321 aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc taaacaagaa
     1381 atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag ggtggcctac
     1441 aatctatgga cttattgaag gactacaagt agaacttggt attactaatc tagtagctaa
     1501 ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat ggggcaaaaa
     1561 tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg ggattaatcc
     1621 aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag agttgcagac
     1681 tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat gggccaaagc
     1741 gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa ttagaaaaat
     1801 gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt gtgatggtat
     1861 ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac taggatattc
     1921 tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt atcctgtgtc
     1981 tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg ttaatggtta
     2041 ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag aagtactggc
     2101 attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg ttgtaataaa
     2161 aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag atacttctac
     2221 gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa tagttggtag
     2281 atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc taactacaga
     2341 ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc aagatggagg
     2401 tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga aggctataga
     2461 tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg ttgatctaga
     2521 catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg tatctgctgt
     2581 aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt gttctcaaat
     2641 aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg gttatagtgg
     2701 gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg aatttaccat
     2761 aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta gagatagcgg
     2821 atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat acaatcagaa
     2881 ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc ttgataatcc
     2941 ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt catgggacat
     3001 catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata ggaataaatt
     3061 aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt caataatagg
     3121 tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca caatcaatga
     3181 tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg gtgaagttaa
     3241 gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg ctgcaggtgg
     3301 aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt ataccttagc
     3361 aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt gtgataaggg
     3421 tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag ataaaatcgc
     3481 ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag gtgtagggat
     3541 aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt ttacttatct
     3601 tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa ttattcaatt
     3661 tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag tgtccttttc
     3721 ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg
     3781 aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta
     3841 tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag
     3901 attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag
     3961 taccacctta tgatccaaga gataattcat ttcaagtaaa aaaatta
//
LOCUS       DAXWLH010000197         4007 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXWLH010000197 DAXWLH010000000
VERSION     DAXWLH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.203"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..730
                     /locus_tag="ACGE36_002528"
     CDS             <1..730
                     /locus_tag="ACGE36_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384878.1"
                     /translation="VVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYT
                     HIIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPI
                     REVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIE
                     TKLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPP
                     VSEEMENKILKLTNFENTTKFEME"
     gene            739..999
                     /locus_tag="ACGE36_002529"
     CDS             739..999
                     /locus_tag="ACGE36_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384879.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            1036..2040
                     /locus_tag="ACGE36_002530"
     CDS             1036..2040
                     /locus_tag="ACGE36_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HFF8384880.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPISVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            2065..2421
                     /locus_tag="ACGE36_002531"
     CDS             2065..2421
                     /locus_tag="ACGE36_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384881.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            2583..2774
                     /locus_tag="ACGE36_002532"
     CDS             2583..2774
                     /locus_tag="ACGE36_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384882.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            2883..3101
                     /locus_tag="ACGE36_002533"
     CDS             2883..3101
                     /locus_tag="ACGE36_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384883.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            3138..3455
                     /locus_tag="ACGE36_002534"
     CDS             3138..3455
                     /locus_tag="ACGE36_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384884.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            3475..3663
                     /locus_tag="ACGE36_002535"
     CDS             3475..3663
                     /locus_tag="ACGE36_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384885.1"
                     /translation="MTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLTLSS
                     FVTRVDQKKVINQKDFKI"
     gene            3660..>4007
                     /locus_tag="ACGE36_002536"
     CDS             3660..>4007
                     /locus_tag="ACGE36_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFF8384886.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDS"
BASE COUNT         1588 a          454 c          818 g         1147 t
ORIGIN      
        1 agttgtaaaa agagaagtga ttgcttctct tagtgagaat gaaaatgacg tagatttgat
       61 taacgacatt ggaaatacaa ttggagatca tgaagctact gctaaggcat tttatgacgt
      121 tgggaaatat acacatatta ttgcggaaga acagttaaga caaaaggatg tagttatcgt
      181 agtgttacag cctgataaag taaagattga aactattcac gatttaaacg aggaacggaa
      241 tagcttagaa agtagtgagg acatgacaga taaatttgtt gggcataagg tttcggaagt
      301 tcccattcga gaagttgtga taaaagattt tgagcaagag ctagaaacaa ctaattatta
      361 tgacaaacaa gcttggcaag agcgcattga atacttagaa aacatggaag aaccagtttc
      421 cagattagca ttagatagtg atattaatga attttatgat agtcataaag aagaaattga
      481 aacgaaattg acaaatttat ccgaaaagtt agagatcgat aaagaagaac tgctaggttt
      541 tccagtagat acttcttcta cggaatacaa aaaagctacg tctatcatgg cttacaacgg
      601 tgcaatgaga gaaatttata atgaaattga aaatgatacg tttgaaatac cacctgtttc
      661 tgaagaaatg gagaataaga ttttgaaatt gactaatttt gaaaatacaa caaaatttga
      721 aatggagtag ataaatgaat gatagaacaa gaaaatagaa attttttaga aacgcaaggt
      781 gaatatcgtc aaattaataa tagtcatttt gtaatttgtg aagatgtaca aatcgctttt
      841 aaaatggctt tgaagtacgg aaaaagtatt agaaaaaaga taattagaga aaaaaagagc
      901 aagaagatag gcttttggga gttatctctt agggatgggc taaactttat tttctgtaca
      961 gaggattatt atgaaaaata caagtgtacc ttatcgtaat tttactagtg atggtctagt
     1021 aaagggggtt tttgtttgga tttgaaaaag gcaaaaaaag taagtatcgt tgatttttgc
     1081 aaacaaaatg ggataggttt aaagcaagat tcaagggaat actatagatt agaagatcat
     1141 gattcttgtg taatcagtga tcgcaaaaac ctttttaaat ggaactcaaa ggatttaggg
     1201 ggagatattt ttgattttat caaagcctac tatgattgtt catttaaaga agcaaaacag
     1261 aaacttgaag atagcgatta tgaagttcat tcgttcaata caaaagtgaa aaaagctccg
     1321 taccaatatc agaaagaaaa tgaagtgcca atatctgttg caaagtctta tttagtaaat
     1381 gagagaagaa tcgatacagc tttagtggaa gattttttat ctaaaggact aattaaacaa
     1441 gataaaaaag gaaatgtttt gttcttatgg aaaaaagatc aagaaatcgt tgggtgtacg
     1501 gaacaaggaa ctagaaaatt ttttcatgaa ggcaaacaaa aggatgttac atggaaaaaa
     1561 atacaagaaa actctaaaga gtattcagga tttaacgtaa cttttggtca accagataaa
     1621 atgtattttt ttgaaagtga aatagatttg atgtcgtaca ttacacaaaa gccagaaaaa
     1681 gccgaaaatg ctacgtttgt tagcatgaat ggattaaagg taggtaatgt attgcatggg
     1741 gttacagagc attataataa gtttaaatca ttgccttcag aaacagtcct ttgtgttgat
     1801 aatgataaag ccggacgtac ttttattgaa actgaattta aagaaaaagg aattggaaga
     1861 gaagggatag gaatcagtga agtaaaagtc gatgtacctt tgcgagaggg atttgattgg
     1921 aatgataatt taacaagagg aaaagaagct tttaaacgct tcaagttaga agatgtatca
     1981 aaaatcgtga acttcacaca taaattatct cttaatactc ctgtatatga aatggaataa
     2041 caagaagaaa gtagttgatg tgttatgaac gatacaatag caatcttatc agattcatta
     2101 attgatttag ataagacgtc tacagactat atgattattg aagaacaact taaaaataaa
     2161 aatccagagt tcgatattca agaaaaatca ttgcgtattg aattaccaaa atttaaacaa
     2221 aaaaaattaa taactattct ctttgagatt attgaacgat atgggcttac ttattatcaa
     2281 atttggacgg ataagaaaga tggccaatat acaaaagggg taagcataga agccgttaga
     2341 gaagacgatc aagtaagtat taaagcagtt caacccttaa tggggtataa cgaagaatta
     2401 ttgaattgtt taaacgtcta gtgtaaacta ggcgtttttt tacattattt ggctacgaat
     2461 agacagttaa ttaaactaaa gaaaaccatt gactacaagt agtcattcct gtatgatata
     2521 gccatagact acaagtagtc aaagactcaa acccttgatt taataaaaag gagagataat
     2581 agatggagca gacaagaatg ttttggtcag atcgaatgca agatcacttg gattgtattt
     2641 acaaaaccag agagcaatta aataagttag atactgtcag atattatttt acctacttca
     2701 gtgagaatga cgaacaaaga tgggatattt ttcatgatgg taattacgaa gaattttttt
     2761 acactgaaag ttaattattt gttgaaatga tgaaggattc tatttgatga aagggaacgg
     2821 aaaaccgttc ccctaaaaaa tatccaaatg actacaagta gtcatggaag gagagcggaa
     2881 taatggtaaa taaaaaagaa acgtttaaag taggtatgga agtacagggc gttgttattg
     2941 ggagcggaga gcttatcgag ggagaagtaa ttagtttgtt agctaatgta gcggtagtta
     3001 aagcaatcgg aaaagcaccg gtagtgatga aaaaatgcag catgatgtgt cttgatggtt
     3061 taaacaatcc aataactcaa cgctttgaga acgtgatata atagaaaaaa gaagattttc
     3121 tactgggaga gattattatg aaagactatg cttcacgtaa atttatccac ttagagccta
     3181 tgaaagaatt ggttattaat gggaatgaat ataatgatta caaatcttta tttgtaaagg
     3241 aacagcctaa aggcggtatt cggatgaaaa atttaccgga cttccgattt atcgaagtaa
     3301 acaatttagg aattatagaa gactatttag ataagttaga tgaagcgata aagcaatcaa
     3361 ttgaaaaaaa tggtgaagat catatactta cggttgtttt aaaagacttg aaagatagac
     3421 tagaagctga agcagagtac tttaataaag actaataaga agggaatgag agctatgaca
     3481 gaggctgtaa aagaattaaa gaaaatgtat cctgatgttt tgaatatgac tgtagatgat
     3541 tttcatgaag ccttaaaaaa tgcagaaagt gaggaagaaa ggactttcta ccttacactt
     3601 tctagttttg tgacacgagt agatcaaaaa aaagtaatta atcagaaaga ctttaaaata
     3661 tgagtttaga aaatgaaaaa ccgtattatg tagttgttgg gggcgttaac ggcgccggta
     3721 agtctactat ttatcaagtt caaaatttaa aaagaaaaga acttgatact gatagaatta
     3781 atgcagatga aattttgaga agttttggcg gaaattggaa aaaacaagca gacataaaca
     3841 aaagtatgcg tgaagttcta agagagatca aaggtcattt taaagaagga aaatcttttc
     3901 accatgaaac gacttttggt ggtaatccat atcccgataa aatgagactt ctaaaggcta
     3961 aaaaattagg ttatgaatcg tttttaatct atataggact agattca
//
LOCUS       DAXWLH010000198         3981 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXWLH010000198 DAXWLH010000000
VERSION     DAXWLH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.204"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..2389)
                     /locus_tag="ACGE36_002537"
     CDS             complement(<1..2389)
                     /locus_tag="ACGE36_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFF8384887.1"
                     /translation="MKKLKLISVALFSLITLSTARQSVVSAEGTSTKAVVEESGDKKV
                     AIDEKVKNEAEKATVVQGADGLMYVVVDENNPEKLLEVESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFETE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRRALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVIFTIYSSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEITQDTPSTKPAKVKVT
                     NIYEVSVSEKVSDMNESQVLKNVWSADPSDYLQYDITTGNLKKSGERNVQSFTIEGFY
                     DNKNVDVSKSEVYVGSTNVTSSFDISNDTGNGKVVARAKATVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKPLTIVKNSKVTVGSKISKSANSNDVETVLYSRKITINHIDE"
     gene            complement(2807..3130)
                     /locus_tag="ACGE36_002538"
     CDS             complement(2807..3130)
                     /locus_tag="ACGE36_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384888.1"
                     /translation="MARSRIAKIEQTKKDQKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKDKG
                     IMIPA"
     gene            complement(3275..3718)
                     /locus_tag="ACGE36_002539"
     CDS             complement(3275..3718)
                     /locus_tag="ACGE36_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFF8384889.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLFHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSIVFYT"
     gene            complement(3721..>3981)
                     /locus_tag="ACGE36_002540"
     CDS             complement(3721..>3981)
                     /locus_tag="ACGE36_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384890.1"
                     /translation="IWKYVVAVFLLVGGIIALVKWAIIPNALVFINWFVPNFWSFFGY
                     ILIIWFMFKVLSFFWSPVYSLLGKWSYLILIATAIYIIMQIN"
BASE COUNT         1217 a          763 c          537 g         1464 t
ORIGIN      
        1 tttcatcgat atgattgata gtaatttttc tggaatataa aactgtctcc acatcattag
       61 agttagcgga tttagatatt tttgaaccga cagtaacttt actattttta acaatcgtta
      121 agggtttttg cttttcagtg ttttcaagcg tagatttttt tattttcata gtgatccgta
      181 aattatagga cttattataa aaatcattat tcgttaacac ggttgccttc gccctagcga
      241 ctacttttcc gttgccagta tcgttactaa tatcaaaaga gcttgttaca tttgttgatc
      301 caacgtatac ttcacttttt gaaacatcta catttttatt atcgtaaaaa ccttcgatag
      361 taaagctttg gacgtttctt tctccggatt tttttagatt tcccgttgta atgtcatact
      421 gaagataatc gcttggatca gcagaccaaa cattttttaa tacctgactt tcattcatat
      481 ccgatacttt ttcgcttaca gatacttcat aaatattggt tactttcact ttagccggtt
      541 ttgtggaagg tgtatcttgg gttatttcaa aattgtattt ttttccatcg ggatcataac
      601 cagtaacacc ttgcttttct actgcataat atttaccaaa ttgtagttca tcaactttta
      661 ttttcccatt actatctgtt tttaagttgt cttttacaac tttattatcg gaactataaa
      721 tcgtgaatat tacattcggc aatcctaatc catctaaatc gcctattttc tcaatttcta
      781 cagaacctgt ggatttctta ttggttcttg tggtagtaac attttgattt tctttaactg
      841 taattgaaaa ttcgcttgaa tctagttggt agccattagg agcttttatc tccttaactt
      901 ttactacagt tccagctttc aacctttcag ttaattttgc tttaccattc gaatcagtag
      961 taacatcttt tgttttacct gagtagctaa atctaaatgt tgctcctgat aatggttttt
     1021 ttgtttctga atcaactttt aaaacttcaa cagttccttt tctcttaaca tttaccgtaa
     1081 cagaagtact aatgtaaaaa gaaccggtaa caagcttttg ataagaacca ttactccata
     1141 ccgttgtagg gtgcgcgtct gaagggtgag aacctttaat catatctaca gtgattttgt
     1201 ttccagtggc tcctgtagta tcaacagtta aagtaccatt gcttatggta gctttgccgc
     1261 caccagatac agatttcaca tgcaaattat gatctccata agtattagtt actttaaagg
     1321 ttgctttgtc tccctcatca accgtcttag acgtagaatc ccattttgtt cttactctaa
     1381 tagcatttgc acgagctact aaatcatttt tccacttagt agctccgttt ttccaatttg
     1441 gcgtaacagt tatactttta ggcttttcat tactgttttc ccaaacaagc atttgagttg
     1501 ctatccatct ttgttttccg gcatcattat tagcccaacc acctaattct ttatatgctg
     1561 ataaaatcga actaatcatt tctctttgcg aattagaaag ataaccactt gttgaaccag
     1621 tgtatgtatt cgtcgcttgt acaccggcct gtaaacaaaa agcttctttc ccatcaatag
     1681 ttcttctaat agcataattt tctgtataac tataaccatt gctcctaatt aaataaatca
     1741 cccacggata accatttgga gggtttattc gtgtttcact tacttgtgaa gcacggttac
     1801 taactaaagc acgccgactc actttcgctc ttttcggtac gtcatttaaa ttattgctta
     1861 actctaaaat cctttgactg aaatatcccc ttgtcttttt cattaacgct tcagtctcaa
     1921 atgtattctt tgtcttttgc aatttttcat ttaattcatc ttccgataaa aaagggaatt
     1981 tcggatcatt caacgctgac tgcaacgaac taatatcgtt gtaatcaata tcgtcaaagt
     2041 ttttcacttt ttcgttagct ttatcagcct tttcttgtct cttttcatca acaccgactt
     2101 caacagcgcc ggtattttct tgttgatttt tcaaaggaga ttcaacttct agcaattttt
     2161 caggattatt ttcatcaacg accacataca tcaagccgtc tgcaccttga acaacagttg
     2221 ctttctccgc ttcgtttttc actttttcgt ctatagcaac ttttttatca ccagactctt
     2281 ctactactgc tttcgtagac gttccttcag ccgaaacaac tgattggcga gccgttgata
     2341 aagtgattag gctgaataga gcaacgctaa tcagtttcag ttttttcatt atcacacttc
     2401 ctttagaaag aaatgtaata aagaatcata acaactcttt actacatttc ttgtagtaaa
     2461 gagttaccct gtgtctagca catttattac tgtgctgaat tttttagtgt caatattctg
     2521 atctctagga cgattttcat aggttcttcc tagatagaaa ctttgaataa ttaccattta
     2581 cgccacctca taattagtag tttcaatgga tcgacttttg tattttttgg cgtgaaatac
     2641 taaccttatc atttctatcg ctaacgtatt tacatacctc ttggcaaagg aaataacagt
     2701 atgcttggtt gctattttgc ttgttagccc tacgctttta tctgtaacat agcaacattg
     2761 ctacagtacg agtagtatta agttttcaag gaacaaattc actgatttag gctggaatca
     2821 tgattccctt atctttacag taagcaagac cagagtttct tgatttccgt ttagccacga
     2881 ttaaaccttt actatccaca atatttctaa tttccggata aagttcgtca atcgttttaa
     2941 aattactgaa gttaccaagc tgtacaaaca gaccattcat tgaaacttta acataacgat
     3001 cttcatcaaa tgttttcaca gaccatgcta tcctcttaac atcttcagct ttaaagaatt
     3061 ttgttttatt tttagaaatt ttttgatcct ttttagtctg ttcaatttta gcaattcttg
     3121 atcttgccaa cagctatcac tcctttaatt tttttactaa atcagttcat acttaattat
     3181 atcatttatg acaatttcta tcaacctgat agataaaaac aaaacaaaaa aaatagataa
     3241 aaaccttgta tttttaggtt ttcgtctatt tttttcaagt ataaaaaact atacttaaca
     3301 gtattacaat taaattgtaa aaacaatgaa tagtaaccgg aacaatcagc cttttatctg
     3361 aattatcata gacaaaagac aaaattaaac tcgtcataaa ataaggaata aaatataaat
     3421 ttaagtggaa tagactaaac aaaattgcaa cgattatata tatgcttctt gtagataaat
     3481 ttgtttttaa ttccaattgg ttttggataa tccctctcat taaggtttct tcaaagaaag
     3541 gagcaaaaat cccacttatg aaaaacgaaa taaacgttac taattcagtt acattgtctt
     3601 ttctttgatt aacaaagacc aacgctatca tcgaaaggaa taaatttact acaagaagaa
     3661 ctaatgtata gcctattatc cttttccaat aaattttaat atcttctaaa atctgcataa
     3721 ttagttaatc tgcataatga tataaatagc tgtcgctatt aaaattaggt acgaccactt
     3781 tccaagcaaa gaatatacag gcgaccagaa aaaagagagt actttaaaca taaaccagat
     3841 tatcaatata tagccaaaaa aactccaaaa attagggaca aaccaattga taaatactaa
     3901 agcattagga attatagccc atttaaccaa tgcaataatt ccacctacca gtagaaaaac
     3961 ggctactaca tacttccaaa t
//
LOCUS       DAXWLH010000199         3879 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXWLH010000199 DAXWLH010000000
VERSION     DAXWLH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.205"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..1317)
                     /locus_tag="ACGE36_002541"
     CDS             complement(<1..1317)
                     /locus_tag="ACGE36_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFF8384891.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPVFNKIDADAETLTK
                     RFGILGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKV"
     gene            complement(1331..1786)
                     /locus_tag="ACGE36_002542"
     CDS             complement(1331..1786)
                     /locus_tag="ACGE36_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384892.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(1789..3735)
                     /locus_tag="ACGE36_002543"
     CDS             complement(1789..3735)
                     /locus_tag="ACGE36_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8384893.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQIFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         1313 a          759 c          534 g         1273 t
ORIGIN      
        1 cactttgttc atgtagatag aagcacctac taaaacaact cctaatccac ctattccaat
       61 tacaccaaag taagatgcta ataaaacaaa caaccctact ggccataatc cacataacaa
      121 catagttact aatcctgctg tccccacatc gaaacttgct aatgtaaaca catcagtgat
      181 aattggtgcc aaccatgttg ccatatataa tgttgtaaca acatacaacg aacatcctac
      241 taatgatctc acaatatttc ctttaaaata aggaatcatt aaacagatgg cgaaagcaaa
      301 gaatgctaaa tctccaaaag gtaatacttt atttcctgga agaattagtg ctagcaataa
      361 tgagattggt acaagcaata gagaagttga cataatagtt ggatgaccca cagttaacgc
      421 tgcatccatt ccaatgttta cttggcgacc atttaacttt ttattcgtaa attctttcgc
      481 cgcttctgca attggcatta aaccttccat aaacattgct accattttag gcataatctt
      541 catcactgcc gcagtcataa ttcctaagtt tccaattcct gctacgtcat agcctgccat
      601 aatacccact acaataccga taattaggcc aataacaact gtgtcaccta aaataccgaa
      661 acgttttgtt aatgtttctg catctgcatc tattttatta aatacaggaa tacgatcaaa
      721 aacccaattt actggtttgg caaatagtac tcctgataat cccatcatat gtgtgataga
      781 gattcctgga tagccgaaaa attcgctaac agttttttgt gtacgatctg ctaagaacca
      841 ttgtaaaatt actgcgacaa tcgcgccaat taagccaaac caaaagtttc ccgtaacaga
      901 ttgagtaatc atacctagga aaattgcgaa ccaaaagttc caaatatcaa cattcaatgt
      961 ttttgtccaa cccaaaacca caaatatgaa gttaagaatt aatgaaattg gaatcattaa
     1021 aattcccaat gatgtagagt aagccaaagg tccaccaaca cctacgccaa tatctaggat
     1081 attcaggcca ccaccgtatt gttcccccat ttgttgaatt gctgttccta aaccatctga
     1141 taataaactt aaaactaatc ctaaaccaat agaaccgata ccaacagtaa cgccggaaac
     1201 aaatgcttta ccaggtttta atccaaataa tatcccgatt acaaaaataa tcagtggtaa
     1261 aaagacgttt gaccctaatc caataaacca ttcaaaggca tttaatacaa tatccatctc
     1321 tctattcctc ctaataaact cctgcagaat gtactaactc aataaatgct tctttcgtgg
     1381 tacatgcaag taattcattt actttttctt cattttgaaa taggctaact aagttttgta
     1441 gtgtatcaat ttgttcatga ggttgtgaca ttgccaacat aaagactaaa cgtacaggta
     1501 tttcatgaga tttatctgtc atatctgaaa ataccagtgg ttcctttaat gaggcaaaac
     1561 agatttgaga gctatttaca tgtgcggaat ctgtatgtgg gatagcaaac ccaatttgat
     1621 ttacttctaa tccagttgga aattcttgtt cacggttggt gataccttct aagaaactat
     1681 ctttcacgca accagcgttt tttaattgtt cagacattac ttgaaacaat tctttttgat
     1741 ctttcacttc taattgaaat aaacataatt ctttatctag atacattttt attctccttt
     1801 ttgttgaaaa attcttagta gtttttcgct atcatgaact tgtaacaagc gatcgaccat
     1861 ttctttttgc ccaataaatt ggtaaagttc ttcatatact cctctaatct cagatacatc
     1921 ttcttcagct aaactaacca taatgactaa tttgactttt cgatcgcccc aattaatcgc
     1981 attttttaat gctacaataa aaatgttgga ttcaatgacc attgatggat tcgcatgcgg
     2041 taaagcaaca ccactttcta aattagtatt ccccattttc tctctagaga agatagaagc
     2101 acgaaactct tcagtaacta atgaattggc ttctagttcg gaaatcatca aatccaaaca
     2161 ttcttctttg ctttccacct gtactcctgt ttttactaaa gatgattgaa caaatttaga
     2221 tatggtggga actttaaccg ctttaggaat cagttcattt tgaataaatc gcgaaggttc
     2281 ttctattaaa tatttagtat aagcttccat aatattcata taatctgttt tacttaccaa
     2341 tggagatacc cttactaagg gtataccacc tgcatcaaag gaaagagagg aaataatcaa
     2401 atcaacttcc ccaatttcag ctaaatgaaa cttatctaaa gttgatattt ctacattatc
     2461 ttttgctggt aaaaaccgtc gaactttatt gtaaattaat tgcgcagaag aaataccgta
     2521 ttggcaaata atgataatat tatttgcttt ggactgatct tcaatagcaa tctgaaaatg
     2581 aatcaaaatt aaggaaactt catcgtccgt taaagtcaca tcaaattctt cttcaaggat
     2641 ggacatggcg taccatacta tgctaaagag ttctgaatat tgagatttga tattttctaa
     2701 taacggattt tgaattttaa tacctttttt taaacgaagg accattgctg gcacatgata
     2761 caataaggat tgatataatc gatcattttg agtcaaatca attttttcaa tctcactcat
     2821 tctttcaatc aatttatgaa cgctcatagc gtactcttca ttgctggttt ttaaattatt
     2881 tgtcacacga tgtgcaaata gttgacgaca tagatattct ttatctgcgt cataaaatgg
     2941 aagattcaat tttgccttca attcttcaat taaaccattt gccacaatgt acgtttccat
     3001 atagcgaatg ctattgaaga ggaattcttc ttcaacagtt aaatgatgac ctttcagtaa
     3061 acggttacat tggataacta aagtagaaat caatgaccga acatagtaat cagaaacctt
     3121 ttcagtcagt tcactgtaat cattgaatag caaatcatga atgacactaa taacttttga
     3181 atcaaacaac atatttaaaa cttgaaaaaa tgtttttgat tccatgtcat tcgcataata
     3241 gaatacaagt tgcttgattg ctcgttgaat tgcttgttca ttaccagtaa aactaattcc
     3301 ttctgcagat gactgcaact taacttctcc actgccaata attttattga taattctcag
     3361 gtcattgtat aacgatgttt tactaaccat aaaaatttga gacaaatgag ttaaagaaac
     3421 aggcttatca ccaaacaaaa cttctttaat gatctcgaca cgtctttttt ctggagaata
     3481 aatatctttt tcaagataat tttctaccga tgaaatcaaa tgttcaacgt ttttcctatc
     3541 acctatgagt ttgataccca aagaaggtaa tcgctcaatt gttacttggt aattaactac
     3601 ataatcttcc aacaattcaa tatcttgata aatggtcttg gtcgacactg ccaatttatc
     3661 actaaaaaaa gaagcgggtt taaagtcttc ttgtcgactt aataatttta ataaatttac
     3721 atgtctttca atcaaacttg ttcacctctt aacgctagca tttgttacct cttttcatta
     3781 tttattctag cactggaacc cccttccctc aattacatct atgtcaataa tacttgtgga
     3841 agtttgttgt aaaggaaatc caaataatct taaaaaatg
//
LOCUS       DAXWLH010000200         3861 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXWLH010000200 DAXWLH010000000
VERSION     DAXWLH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.206"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            4..894
                     /locus_tag="ACGE36_002544"
     CDS             4..894
                     /locus_tag="ACGE36_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFF8384894.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(967..1791)
                     /gene="nadE"
                     /locus_tag="ACGE36_002545"
     CDS             complement(967..1791)
                     /gene="nadE"
                     /locus_tag="ACGE36_002545"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFF8384895.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1803..3275)
                     /locus_tag="ACGE36_002546"
     CDS             complement(1803..3275)
                     /locus_tag="ACGE36_002546"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFF8384896.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1127 a          793 c          681 g         1260 t
ORIGIN      
        1 tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa
       61 acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca
      121 aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca
      181 aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa
      241 atcgatatca gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata
      301 cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat
      361 gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc
      421 cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca
      481 gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca
      541 aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg
      601 acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat
      661 aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg
      721 aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg
      781 catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt
      841 aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt
      901 tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct
      961 ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt
     1021 cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag
     1081 ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt
     1141 cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc
     1201 ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc
     1261 accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa
     1321 tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt
     1381 gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg
     1441 tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag
     1501 agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa
     1561 ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg
     1621 gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg
     1681 atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc
     1741 aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc
     1801 tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt
     1861 ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt
     1921 ttatactctt cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt
     1981 gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct
     2041 gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct
     2101 tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca
     2161 aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg
     2221 cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg
     2281 aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat
     2341 acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc
     2401 gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta
     2461 gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct
     2521 aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg
     2581 actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt
     2641 ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc
     2701 ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag
     2761 actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg
     2821 atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc
     2881 aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt
     2941 tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa
     3001 ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata
     3061 tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa
     3121 accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa
     3181 tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc
     3241 agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt
     3301 ttcatttcct acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc
     3361 tgaaaaagca catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact
     3421 atcctttctt attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc
     3481 cgagacacga agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc
     3541 ctactacaat cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta
     3601 caacataatc aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt
     3661 atccccaaag aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat
     3721 gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag
     3781 atacaaagta agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca
     3841 gtctcgcctt gttttttatt g
//
LOCUS       DAXWLH010000201         3830 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXWLH010000201 DAXWLH010000000
VERSION     DAXWLH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.207"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1135
                     /locus_tag="ACGE36_002547"
     CDS             <1..1135
                     /locus_tag="ACGE36_002547"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8384897.1"
                     /translation="VILAIFLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRAL
                     GESKIFVMSGIIAAGINLLLLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQ
                     YFATSSFDFLFLKSMLKYSIPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKF
                     SSIILTLTSIVSMSMIEDAVITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAY
                     SFITNPDYKKSLVLVPILVIMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVG
                     TYLLGYSFGVIGVSFATLLGTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFV
                     CIHRGFYFNLFLTIIFSAIFVFTYKQLIITYLKKFFRIKMAKR"
     gene            1221..1649
                     /locus_tag="ACGE36_002548"
     CDS             1221..1649
                     /locus_tag="ACGE36_002548"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFF8384898.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            1737..2903
                     /locus_tag="ACGE36_002549"
     CDS             1737..2903
                     /locus_tag="ACGE36_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFF8384899.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1280 a          508 c          702 g         1340 t
ORIGIN      
        1 agtgatatta gccatttttt taccaataat atatagaatg attttcccga atttagagta
       61 ttatcaattg tttgtttttc aattaatttt cactcctatc ctaagtgttt ttcaatatat
      121 tgtaagagca ctaggtgaat caaagatttt tgtgatgagt ggtataatcg ctgcgggtat
      181 caacctgtta ctccttacta tttttgttgt tacaatgggt atggggatac ttggtctctt
      241 actctctatc ataatttcta atgttataaa tttgattttt ttgattttca gggggcagat
      301 ctttcaatat ttcgcaacga gttcttttga ttttttattt ttaaagtcca tgctgaaata
      361 ctctatccca ctcactttga atctcacttt tggatggcta atagttggtt tttcaagatt
      421 ttatattgga acgtacatcg gagtaacaga aaatggtatt ttttcttttg cgatgaaatt
      481 ttcatctata attttaactc ttacaagtat tgttagtatg tctatgatag aggatgcggt
      541 tatcacgtct gagaatgatg atttcatcga aagattcgtt gagaaaaata cctatatttt
      601 taaaatgtac attaggttag cgactctttc tattcctatc atttgctgtg catactcttt
      661 tataaccaac cctgattata aaaaatcatt agttctagtt ccaatattag ttattatggc
      721 atgtttcagt gggatgacaa caaatatcgg aaatatattt gcagtataca ataaaacaaa
      781 tcagttatta atgatttcag tagtgtcagg tctattgagt gttgttggca cctatttatt
      841 aggctattct tttggagtaa ttggtgtctc atttgctact ttgctcggta ctttagtatt
      901 atttggactt agatattatt ttggaaggaa aattgcttat tatgcatttg attggaagcg
      961 aatatcgcta cttttagttc tctattccgc aatcgctttt gtatgcattc atagaggctt
     1021 ctattttaat cttttcttaa cgattatttt tagtgctatt ttcgttttta cgtataaaca
     1081 attaattata acgtatctaa aaaaattttt cagaataaaa atggctaaaa ggtgagggta
     1141 tacgaagtaa gaatagaatc tttcgaagaa taaagagaaa actatttgga aaaaatctgc
     1201 gagaatgtat taatgaaggt ttgatattcg gaaaaaaaca tagtttagta gaggttcctg
     1261 aatttgggag cgagccatat ttaattgaaa taggggataa tttcagatgt tcatttggtg
     1321 ttaaatttat aacacacgat ggtggagtct gggtcttaag gaagatgagt tctgtgagcg
     1381 agtcaacttc aaaaataggt aggatcaaat tgggaaataa tatttttatt ggtatttact
     1441 ctattgtttt cccaggagtt tcaattggcg ataatgtaat tattagagcg aacactttag
     1501 taaataaaga tatcccaaat aatagtgttg tagcgggtag ttcatgtaaa gtgatttcaa
     1561 ctatagagga gcattatgag aaaaataaaa atgaatttat taaatatcga acctcgatga
     1621 ttttgaagca aaaaaatttt ttctattaaa taatattgat tgaataagga atatgttcaa
     1681 aaggataaca gctaaaatac tataaaatga aatttgattg gagcgtatgg aagaatatga
     1741 aaatttcagt tttcggatta ggctatgttg gtctagcaaa ttcactatta ttagcacaaa
     1801 aagagcaggt caaagcttat gatatcgtgg aagaaaaaat cacgatgtta caaaatggca
     1861 tctcaccaat agaagataaa gaagttcatg aatttttaaa acgggatgac ttaaatgtcg
     1921 aatttacgtc aaattttact gatgcagtaa acttcggtga ttacttgatt attgcaactc
     1981 caacagatta tgacgaaaag aaaaactatt ttaatacatc aacagtagaa gatgtaattg
     2041 agaaggcttt agcaattcgt ccagattcaa cattcattat taagtcgaca gttccagttg
     2101 gctatgcaaa tgaaattaaa gcgaaatttg gtacagaaaa catcattttt tcacctgaat
     2161 ttttgcgtga aggaaaagct ttgtacgaca acttatatcc atcaagaatt atagttggag
     2221 aatgttctgc tcgtggtcgc gagattgcaa ctatgtttca aagaaacgca tttaacaaag
     2281 atgcatcagt tcttttgact cactcaactg aagcagaagc tattaaatta ttctcgaata
     2341 catatttggc attgcgagtg gcgtatttca atgaattaga tacgtatgca gaatttcgtg
     2401 ggcttgatag taagcaaatt attgaaggtg tcggtttgga tccacggatc ggttcgcatt
     2461 ataataatcc ttcttttggc tatggtggat actgtttacc caaagacaca aaacaattaa
     2521 gagccaacta tgaaaaagta ccaagcaata tcatcagtgc aattgttgat gcaaatacaa
     2581 caaggaaaga ttttattgct ggacagattt tggcgaaaaa accaaatgtt gttggaatct
     2641 atcgtttaac gatgaaggcc ggttcagata attttcgata ttcgtcgatc caaggaatta
     2701 tgaaacggat taaagccaaa ggaattgaag tggttgttta cgagcctaca ttggatgaag
     2761 atgaatttta tcattcacaa gtattgaaag atattaatga atttaaaaat atttctgatg
     2821 tgatcgtatc taatcggttt gaagatattt taaaggatgt tgaagaaaaa gtttatacca
     2881 gagatatctt tggtgataac taaataataa tgaacgggta catggaggaa aatattttag
     2941 atggtgactc gaaataaatg tttgttctca ttgcatctgt atctataccg ataagtttaa
     3001 aaattccgat taggaaaatg aagttggcta tccttgcgag gaatttattg ctggattatt
     3061 ttatgaacta aagcaagaca tcatcagaaa aaaatccaaa atttgacaat gggattgatc
     3121 gtttaccagc tgtaagcgaa gtatctgaca cgaaaatgga aaatgcaatt actgttatta
     3181 ccaatagctt tttttcatcg atcaagtatt tgaaaacatt atttgtttgc caagctgatc
     3241 aatgttcttt cagacttgga agaacattga tcagctcaca agtaatgatt gaagtttagg
     3301 ttatgggata aaggataagt gaaataaaag tcgtgtctgc atctttgtta gtaccagtaa
     3361 atttttctac tacggcgcta gtcacgacag tccaggcatc tgaaattgaa gatgcaaata
     3421 gagtttctgc tagtttaggt attgatgtta ctgtattagc attaatctta gagccagtag
     3481 catccaatag gtgtagctat agttctaata gtataaaaca atgcagagga tatgattgct
     3541 ttatgtctcg gtgtgtcagg tcaaaataga gttccatatg gggcgatttc tcaatgaata
     3601 atcttataga tataatcttg tttgctattt cgttatcact tttacttttc ttcgattatt
     3661 tcaaaaataa aaatccaaaa tattttaagt tcttactatt tatcattact gtactagctg
     3721 ttttatcgat gacagatttt atttaactta tctcaaaaag tagggaacca tatcattttt
     3781 cagtaacatg cgtattattt gtataaataa tacaaaatga aaaattatga
//
LOCUS       DAXWLH010000202         3777 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXWLH010000202 DAXWLH010000000
VERSION     DAXWLH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.208"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE36_002550"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE36_002550"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFF8384900.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACGE36_002551"
     CDS             complement(1172..1867)
                     /locus_tag="ACGE36_002551"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFF8384901.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACGE36_002552"
     CDS             complement(1880..2596)
                     /locus_tag="ACGE36_002552"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFF8384902.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACGE36_002553"
     CDS             2926..3531
                     /locus_tag="ACGE36_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFF8384903.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
BASE COUNT         1078 a          774 c          670 g         1255 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcg
//
LOCUS       DAXWLH010000203         3751 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXWLH010000203 DAXWLH010000000
VERSION     DAXWLH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.209"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            403..609
                     /locus_tag="ACGE36_002554"
     CDS             403..609
                     /locus_tag="ACGE36_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFF8384904.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(811..1812)
                     /locus_tag="ACGE36_002555"
     CDS             complement(811..1812)
                     /locus_tag="ACGE36_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8384905.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1817..3730)
                     /locus_tag="ACGE36_002556"
     CDS             complement(1817..3730)
                     /locus_tag="ACGE36_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8384906.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
BASE COUNT          989 a          763 c          585 g         1414 t
ORIGIN      
        1 tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct tgataacact
       61 ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg atatgattgt
      121 tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt ttgggggtat
      181 cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg tatgatggta
      241 aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca tctttgtttc
      301 aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt tatagattct
      361 taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc tatccatatt
      421 tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg ttcccagcct
      481 tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc actcggcttt
      541 ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg atcaaaacaa
      601 caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg tgatattccc
      661 aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa gttacaatat
      721 tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga gctaagtgac
      781 agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta ctaacttttg
      841 atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt tcaaaacagc
      901 atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa atacagaagt
      961 tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga atgtgttttg
     1021 cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg attgttcaaa
     1081 gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag agattttatc
     1141 acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa acttcgtaat
     1201 ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt cattttgaag
     1261 tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa tcgaaatatt
     1321 cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt cgttcaattg
     1381 gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat ttacgatgat
     1441 ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt tatcccctcc
     1501 tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt ccactttcgg
     1561 tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt ctttttttat
     1621 atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt gttctttttc
     1681 acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga tttgattaat
     1741 caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca ctattcgctt
     1801 gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata atcgtgctaa
     1861 cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca gttttcggca
     1921 acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga atcggttgcg
     1981 tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct gcattctctg
     2041 tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa ataatagttg
     2101 gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat tcgtatcctt
     2161 cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc tggatttcgt
     2221 cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt atctgatcat
     2281 ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg tcaacttcct
     2341 gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct cctgataact
     2401 catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca tggataatca
     2461 atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta tccaaatgca
     2521 tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca tgctcttgat
     2581 ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct actgtctggc
     2641 tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca ataattcctt
     2701 tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct cccatcgaac
     2761 caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt atccagtaaa
     2821 ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta ttaccagtcg
     2881 ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg tattcttctg
     2941 taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact gttttatctg
     3001 aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt ttgtttagta
     3061 cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta ggcgttgtcc
     3121 catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa gcgatatcgt
     3181 acaatagata agtagcaatc agatgtttga taatctcttt atctacacca taatcattaa
     3241 cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct gttggtccat
     3301 taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca ttgaaatttt
     3361 cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact ttagcagaag
     3421 aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg ttatctgtgg
     3481 tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt tcagtatttg
     3541 tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt tctgtggttt
     3601 ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc cattgcggga
     3661 cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt tttgttctca
     3721 agctattcac tcctttttat gtttctagga g
//
LOCUS       DAXWLH010000204         3674 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXWLH010000204 DAXWLH010000000
VERSION     DAXWLH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.210"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..376
                     /locus_tag="ACGE36_002557"
     CDS             <1..376
                     /locus_tag="ACGE36_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HFF8384907.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
     gene            398..1534
                     /locus_tag="ACGE36_002558"
     CDS             398..1534
                     /locus_tag="ACGE36_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFF8384908.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            1549..2199
                     /locus_tag="ACGE36_002559"
     CDS             1549..2199
                     /locus_tag="ACGE36_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8384909.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATSSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDST
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            2213..3109
                     /locus_tag="ACGE36_002560"
     CDS             2213..3109
                     /locus_tag="ACGE36_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384910.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMMIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            3145..3375
                     /locus_tag="ACGE36_002561"
     CDS             3145..3375
                     /locus_tag="ACGE36_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384911.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
BASE COUNT         1470 a          454 c          688 g         1062 t
ORIGIN      
        1 tatcaattca aataacaagt atgcttgtga atatgtacta agttttgagc gagaaatgcg
       61 aaaaatgcgt gcaggtgttt attttgcaac tcaaacgcct gaagaaatta tccctaaaga
      121 ttctggatca gaagtagcag atgtgattag aaacattttt aatctatgta catttaaatg
      181 cttctttaat ttagattctg cattgttgga agacataaaa aaagttttag gaaatacaat
      241 tactgataca gaaattatgc ttttaccaga attagaagta ggacaagctg tagttcaaac
      301 ttccagtgaa gacacctata tgattaattt tgatccatat ccagagcaaa ttgaacgatt
      361 cgatggtggt caataataga aatcggaggt gaatttaatg gagcagaaca aaaataataa
      421 tgttgcttca acactcgctc gtcaagggat gaagcaagta tcaaagaaaa taggtcttgc
      481 tttaaaaata aaaatagggg tagttatagg actgataaca ttggttggcc ttttagttat
      541 aggttcttta gctacattac aaggaacaca aactgcaact aatacaggag gtgcagaagt
      601 cattggtggc aacaaaccgt tgtcacctga agttcaaaaa tggcaaaaaa cggtagaaaa
      661 atatgctgaa aaatacggaa ttaaagaatt agtaccttat gctttagcaa ttatgcaatg
      721 tgaatccgga ggtaaattgc cggatttaat gcaaagtagc gaatcagccg gattgcctgt
      781 aaacacactt caatatgaag aaagtatcga tcaaggactt aaacatttga gtaatgtgta
      841 taacattgct aaaagttatg gcatggaaag taactatgat ggaatagttc aagcttacaa
      901 ttacggcata gcttacatgc actacttagg aaaaaacaaa aaaacacata ctatcgaaac
      961 ggctgaagcc tactctaaaa atgttgttgc tccaagttta ggaaatacta caggacagac
     1021 ttattcatat gtaaatgaag tatcaattaa atatggaaaa acatatttgt atttaaatgg
     1081 tggtaatttt tactatgcag agttagttaa acagtatatc ggtgggggta gtggtaatcc
     1141 ggctaatcct tcagaatttt ggggaaaaat gaaaacagaa atggaaaaat atcaaggttg
     1201 gccttatgtt tggggcggta aaaatccaac gacaggtttt gattgtagcg gattaacagc
     1261 ttatctttat caatttagcg ccggaattac aatagatagt tatacagtaa cgcagtatga
     1321 agcaactgaa gcagtatcaa ttaaagaagc aaaagctggt gatctgattt tctttagagg
     1381 aacatatgga gcgcctgatt tcatttctca cgtaggtatt tatgtcaatg aaacaactat
     1441 gtatgacagt aattcaagcg gaattggtta tcacaattgg caagattcat attggcaatc
     1501 acatagtcca gagattcgta gagtcaaaaa ataggaggaa gaaaatagat gattaagaga
     1561 aaaaatgata ataaatggaa atatatttgt ggtgttttaa ttattatttt agttttaggc
     1621 ggattgttat ctcaacttca tacaagtaac attcaagcaa aaaataaaaa attggatcaa
     1681 gaagttacac aactagaaga acaaaatagc aagttaagta aaaatattag tgatctaaat
     1741 gcgaatgtag aagcgaactt aactaatgat gatggtagtg aattaaataa acaaattgct
     1801 gaagttgcta agaaatttgt tgagctatat ccggtatatg atattgaaaa agtagaagaa
     1861 aagaaagctg atttagagaa aatagctact tcttctgtag ctgattctat cgtgccggat
     1921 gacatgatta caacgagtaa aaagacttta gaaagtaaat ctactactca aaaaggggaa
     1981 gcatattcaa gcgatccaac atttaaaagt aaatataaag attcaacatt atatacgaat
     2041 tatgttaacg ccaatactat taattatttt gctgaagtca attataaaac agaatcttct
     2101 agtggagata cagaaaatac agtctatatg ctttttgaag taaccaataa agacggccaa
     2161 gtgttggtag ataattatga aatcaagtac ttaaaatagt gaggtgaagt atatgaataa
     2221 gaaatattcc attcataata agaaggaacc aaataaaaaa aataaggcac aaattgacaa
     2281 agtatataaa aaaacgaaag gagtaaaatt atatttaggt gctatacttg gatttttagc
     2341 aataggaatg atgatcgttt tttctcctta tgtgatgggg aaaagttatg attttgaaac
     2401 tgtaaaaatg agttcagccc aaaatgtaac gtcttctttg aatatgtatg ttgatgatat
     2461 ggaaataaat agggaagccg gactgtttaa attagtgttg aggtatcaag atgatacagg
     2521 tactaaatca ctaagtaata ttaaaagtga ctataaatta aattttataa cgaacaaaaa
     2581 taatgaagat gttaagacaa aagttgtaaa attgtcagat gaatacacgg taatttatta
     2641 tcaaaatcta cctaaagatt ttggtgtaat ttctgtgaca gttaacccta gatatattta
     2701 tcctgaatta gaaccttcaa atgacttaaa agaaaaggaa ataaaattct atgcagtaga
     2761 caaagatatt aaatcaaatg ctaaattgga agttgaaagt acttctgatt tgcaaaaaga
     2821 taattttaaa tatcagatta aagctataga aaattctata aaagatgaga aaaaatcaat
     2881 tgaaaaaatg aatttagcaa ataaagtagt gcaaaaagat attgataaag ctaatcaaaa
     2941 cttagaattt caaaccctag acgaacaaac agatacaaag aatgaaatca gttctatgca
     3001 gacgactatc gaaaataata aatcttcaat agaggaaagt gaaaataaaa tcaaacaatt
     3061 tgaggaaaaa ataaagcaac tagaaaagtc tgcaagccga tttgagtgat cgtgtttaaa
     3121 ttattttaag ggagtttaga gagaatgtgt aattctattg tcactattca agagaaagct
     3181 gaagaagaat taatgagaat tgaagcggaa tataaagaaa agagaaaagc aattaagcgc
     3241 aaaaaaaatg cagagcttaa aaaatataaa gaggaacaag cgttaaaatt aggaatgatg
     3301 ttgtgtaaat attatgattg ttatgatata aacgaagtgt taaaaaaagc gaatttaaac
     3361 caattttgtg aatagaaaag agagtggcaa ggtaaaactt gtcactcttt ttttgtgcgt
     3421 aaaagcttta gttactatga ccaaaactac ggacgaaact atgaccaaaa gtaggtacta
     3481 tattatgacc gaaaataggt cgaaaatgga tttatttgtg atagcaaaaa atatgaagtg
     3541 attttgaaag aaaaccagtt ggcacagcca acttagccac cttcctttag gaaggtgcta
     3601 tcagttttcc cttatgctct ttgcattaat tctttggttt cgtctttaaa tctttaattc
     3661 tcctttagac tttg
//
LOCUS       DAXWLH010000205         3663 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXWLH010000205 DAXWLH010000000
VERSION     DAXWLH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.211"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..276
                     /locus_tag="ACGE36_002562"
     CDS             <1..276
                     /locus_tag="ACGE36_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFF8384912.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACGE36_002563"
     CDS             419..640
                     /locus_tag="ACGE36_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8384913.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACGE36_002564"
     CDS             656..1498
                     /locus_tag="ACGE36_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384914.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACGE36_002565"
     CDS             1561..3363
                     /locus_tag="ACGE36_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFF8384915.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="ACGE36_002566"
     CDS             complement(3422..>3663)
                     /locus_tag="ACGE36_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8384916.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAXWLH010000206         3657 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXWLH010000206 DAXWLH010000000
VERSION     DAXWLH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.212"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            180..1895
                     /locus_tag="ACGE36_002567"
     CDS             180..1895
                     /locus_tag="ACGE36_002567"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384917.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1898..>3657
                     /locus_tag="ACGE36_002568"
     CDS             1898..>3657
                     /locus_tag="ACGE36_002568"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384918.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTA"
BASE COUNT         1088 a          721 c          723 g         1125 t
ORIGIN      
        1 catgttttct cattttcttt tttttaaaaa aaatagtcta ttcaatttct aaagcttctt
       61 cataataaaa ctttcattta tgaaaaggct tgttctcttt tcattctaaa tttcctaagc
      121 tacaatgtgg tagtacttgt tttttcaatt gtggacaaat aaataatagg aggaatcata
      181 tgattcagtt attacgttat gcaaaagact atcgtaagca aatcattttg ggcccgttct
      241 tcaaatttct tgaagctgtc ttcgaacttg tcctgccttt aatgatggct tccttgatcg
      301 ataatggact aaaaatgaat gaccgaggaa aaatcattga aatgggctta tggatggtcg
      361 ctatgtctgt gattggtctg atttgtgcta tcatttgcca atactatgct tcagtagctt
      421 cacaaggatt tggtacagag ttacgtaatc agctaatcaa aaaaatcaat actttctctc
      481 acaacgagtt gaatcatttt ggaacagata ccttgatcac ccgaatgaca aacgacatca
      541 atcagcttca gctagcttta gcaatggtca ttcgtttatt gatcagagca ccgtttttaa
      601 gtatcgggtc agtggtcatg gcttttgtga tcgactggga agtaggtctg tttttcttag
      661 cattattgcc tattttctca atcattttat tttttatcat caaaaaaacc gttcctctct
      721 accaaaaagt gcaagaaaaa ctagatcagc tcaatgaaca cgttagtcag aatcttagcg
      781 gtgttcgtgt catccgcgca tttgcgcaga caaaaaaaga aacagagaag ttcgataaaa
      841 gcacaaatga tctagcgaac aattatttac gtgtttccaa tatctctgcc ctattatcac
      901 ctgctacaac cttgatcatg aatgtcggga tcatctgttt gctgacagtt ggcgggatca
      961 aagtaaatat tggtagcttg caacaaggac aagttcttgc tttgatcaat tatatgaatc
     1021 aaatgctttt ggcactgatc gttgtttcaa acctcgtcgt gatcttcaca cgtgccgaag
     1081 cttcaggaaa tcgggtgaaa gaggtgctag acacagaaaa ttcgattctt gaagctgagg
     1141 tcagtactac tcccgatttt tcttctgaag caattatcgc ttttgaccat gttgacttcc
     1201 gttatactcc tgattctggt ctttctctcc aaaatattac atttcagctg aagcgaaata
     1261 cagtccttgg aatcattggt ccaaccggaa gcgggaaaac gactttgaca caattgattc
     1321 cgcgtttcta tgatgtcagt tccggtacag ttataatgga cggaatggat gttcgttctt
     1381 ggcagctgga tcatctccgt cagcaaattg ctcttgttcc ccaaacttct gtactattta
     1441 ccgggactat tcgtgaaaac cttcaatggg gtaagaaaga cgccacagaa aaagaatgtt
     1501 gggaagctct gaaaatcgct caggcagaag aatttgtccg tcagcttcca aatggtttag
     1561 atacaaaagt catggaaaat gggaaaaatt tctctggcgg acaaaaacag cgactgacga
     1621 ttgcaagagc tttgattgcc aagccagcat tactgatttt agatgactct ttaagtgcgt
     1681 tggattatca aacagacttg gatttgcgta aggcgctaca gacttatctt ggcagtacag
     1741 tgattattat ctcccaacgt gtccgctcta ttcaagaagc acatcatatc ttagtgatgg
     1801 atcaaggtaa gattgctgct cagggtaccc atgaagaatt acttgctcaa tcagcagaat
     1861 atcaggaaat cgtcgcatca caggaggaag aatagttatg aaagtaaaaa aattatcctt
     1921 gcagacgctc cgccgattca ttccttacct tactgcctat gcaaaagaca tttggctagc
     1981 tgtgatacta ggaatcatca atgggattgc aacagttgtg atgacgttac aaattggtca
     2041 aagtatcgat caaatgattg gagaaggaca ggtccgcttc aatcgactgt tccatttact
     2101 tattctattc ggtggtatcg ttttaatcaa cgtagtcagt caatggctca tccaagtgtt
     2161 aagcaatcgg ttagcttact tatccgttgc aaaattaaga aaagatgcat ttgctcatct
     2221 gaatcgattg ccacttcgtt attatgatca aaactcccac ggaaatatcg tcagtcgatt
     2281 tacaaatgat atggacaata tttccattgc aatttctgcg gccttcaatc aattattttc
     2341 tggagtggca atcatcattc tttcgtttat ttttatgatg cgtctaagtc cacttctgac
     2401 attggtcgtt ctttgttcta caccaattat tttccttgtg agttggatcg ttgctcgtgc
     2461 ttcacaaaca aatttcgata gacagcaaag gattattggg gatatctcgg gctttgtgac
     2521 cgaacgaatc ggtaaccaga aaatcgtgaa agcttttcag caagaagaag ctagtcaaca
     2581 gcaatttgaa ctgctaaatg ctgatctata tgaaaaagga caacgggcac aattttcttc
     2641 ttccttgaca aacccgtctt ctcgttttgt agatcacctt gcttatttat cgattggttt
     2701 agttggcggc ctattggcat tgaagagtgg ttcgtcaatc actgttggtg tgatttccag
     2761 ctttacgatc tactcaagtc agttttctaa accatttatc gaactttctg gaatcacgac
     2821 acaactgcaa accgcttttt ctggtctaga acgagcattt gatttgatcg atcagccaga
     2881 agaaaagcct gatttgccag atgcctatgc cttggaaccc aaaaatatca aaggaaaagt
     2941 ctcttttgat catgtttctt tttcttatga ccccaagcag cgtttgatcg aagatttcaa
     3001 ttttactgct tatccaggtc aaaccattgc tatagtagga aaaacagggg ccggaaaatc
     3061 cacacttgtt aatttgctga tgcgttttta tgacacaaca aaaggaacaa ttacagtcga
     3121 tgaccatgac atccgctcta tccaacgtga tacattacgt aaaagttttg gaatggtgtt
     3181 acaagatact tggctatttg acagtacatt gcgagaaaat cttcgttttg ggaatccgga
     3241 agcaagtgac gcagaaatca atgatgccct aaaagcctct tatatgtatg aatttgtaga
     3301 acgtttgccg gaaaaattag atactaagat tggcggtcaa gggttaaaga tatcagatgg
     3361 acaacgccaa ctgttgacga ttgctcgaac gatgatcagt aatcccccta tgttgatctt
     3421 agatgaagct accagctcag tcgatacatt gacagagaaa aaaatccaac aagccttctt
     3481 agcaatgatg gaaggaaaaa ccagttttgt gattgcccat cgcctgtcca caatcaaaaa
     3541 tgcggaccag atattagtga tggatcaggg agctattgtc gaaatgggca cccatgatga
     3601 gctgcttcaa aaagatggtt attaccgacg gctctatcaa gcacaattca cagcaca
//
LOCUS       DAXWLH010000207         3630 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXWLH010000207 DAXWLH010000000
VERSION     DAXWLH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.213"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            281..496
                     /locus_tag="ACGE36_002569"
     CDS             281..496
                     /locus_tag="ACGE36_002569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384919.1"
                     /translation="MIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYLVAPSCFIS
                     KQVHYTILEELTERQALKTIAKRCDFQ"
     gene            653..910
                     /locus_tag="ACGE36_002570"
     CDS             653..910
                     /locus_tag="ACGE36_002570"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8384920.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            997..2235
                     /gene="istA"
                     /locus_tag="ACGE36_002571"
     CDS             997..2235
                     /gene="istA"
                     /locus_tag="ACGE36_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFF8384921.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2225..2965
                     /gene="istB"
                     /locus_tag="ACGE36_002572"
     CDS             2225..2965
                     /gene="istB"
                     /locus_tag="ACGE36_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFF8384922.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            3384..3539
                     /locus_tag="ACGE36_002573"
                     /pseudo
     CDS             3384..3539
                     /locus_tag="ACGE36_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1178 a          725 c          691 g         1036 t
ORIGIN      
        1 tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga gtcgtctaaa
       61 acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag agctcttcac
      121 tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata aggacaagaa
      181 agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat tttgtaaaga
      241 atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct taaatcgcag
      301 tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc aatcatgcgg
      361 gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt cgaaacaagt
      421 tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg
      481 ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc aacgctgcga
      541 cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt
      601 ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct ctttgacatt
      661 ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc
      721 tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt cattagtcaa
      781 agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc aacacttgaa
      841 caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg
      901 gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta catttttgta
      961 tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat acgcgaagga
     1021 gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc aaaacaatac
     1081 gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa agaatcaaaa
     1141 cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat catccaagac
     1201 aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca caaaggattt
     1261 ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc gaaagtacac
     1321 aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga ctggaaggaa
     1381 gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt tttgtacgtc
     1441 ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca agacacactc
     1501 tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga aatctggttc
     1561 gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc gcatttcaac
     1621 aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg tcggccattt
     1681 cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc
     1741 tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt
     1801 gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg ttggcagacg
     1861 aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta ttttgaagac
     1921 tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg caggtactct
     1981 gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa agaagaccaa
     2041 gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac aaaacagctg
     2101 aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa
     2161 gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct ggtggaggtt
     2221 gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc ttggtttgaa
     2281 tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg agctttcttt
     2341 aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata agcaaaaaat
     2401 gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag agtttgattt
     2461 tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca tgggctttgt
     2521 ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga cacatctgac
     2581 gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca tcacctgtca
     2641 tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc gtgttctcaa
     2701 acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc cgattcaaaa
     2761 acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc gctctacgat
     2821 cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt ctgagatcgc
     2881 agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca caggcaagtc
     2941 ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat tgccgaaaac
     3001 ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat caaaaagaca
     3061 cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt ctataatcag
     3121 atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg cgctatttaa
     3181 cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa atggcttagc
     3241 agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact tcgagaaatg
     3301 accaaagaag ttccctcgaa acttcactat cttaaaaagc ctttttcata tctgaaaaag
     3361 gcttttaact agcattttcg aaaaagtatt ttaatacaaa attagagaat cttcgcacac
     3421 atatcaaaac attgaaacga tttgcttatg gattttgcag ttttaccaat atgcgcttgt
     3481 ggatttttct aatcaacggg ttaataagct atgcattaaa agctagtaaa tcttactaac
     3541 tttgttgatc ataaatgacc ttatcagccc gatttgacaa agaactactt ttttacaaca
     3601 ttattttatt ttcgaataat ctttcttact
//
LOCUS       DAXWLH010000208         3491 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXWLH010000208 DAXWLH010000000
VERSION     DAXWLH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.214"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..2303)
                     /locus_tag="ACGE36_002574"
     CDS             complement(<1..2303)
                     /locus_tag="ACGE36_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8384923.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACGE36_002575"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACGE36_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          962 a          778 c          558 g         1193 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa a
//
LOCUS       DAXWLH010000209         3446 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXWLH010000209 DAXWLH010000000
VERSION     DAXWLH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.215"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..460)
                     /locus_tag="ACGE36_002576"
     CDS             complement(<1..460)
                     /locus_tag="ACGE36_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8384924.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACGE36_002577"
     CDS             complement(500..1552)
                     /locus_tag="ACGE36_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8384925.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACGE36_002578"
     CDS             complement(1565..2503)
                     /locus_tag="ACGE36_002578"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8384926.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2954..>3446)
                     /locus_tag="ACGE36_002579"
     CDS             complement(2954..>3446)
                     /locus_tag="ACGE36_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384927.1"
                     /translation="LISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKG
                     QEIIDSLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENI
                     ILRTEYGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYS
                     HRQ"
BASE COUNT         1156 a          723 c          505 g         1062 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     3061 tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     3121 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta ttgagttgat
     3181 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     3241 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcacttca cctaatgaat
     3301 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3361 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     3421 tactactaat ccaatccgag attaat
//
LOCUS       DAXWLH010000210         3386 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXWLH010000210 DAXWLH010000000
VERSION     DAXWLH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.216"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(559..1599)
                     /locus_tag="ACGE36_002580"
     CDS             complement(559..1599)
                     /locus_tag="ACGE36_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8384928.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="ACGE36_002581"
     CDS             complement(1948..2274)
                     /locus_tag="ACGE36_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384929.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="ACGE36_002582"
     CDS             complement(2261..3049)
                     /locus_tag="ACGE36_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8384930.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1032 a          565 c          467 g         1322 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaaga
//
LOCUS       DAXWLH010000211         3322 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXWLH010000211 DAXWLH010000000
VERSION     DAXWLH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.217"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..93
                     /locus_tag="ACGE36_002583"
     CDS             <1..93
                     /locus_tag="ACGE36_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8384931.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="ACGE36_002584"
     CDS             complement(151..1371)
                     /locus_tag="ACGE36_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8384932.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="ACGE36_002585"
     CDS             complement(1391..2002)
                     /locus_tag="ACGE36_002585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8384933.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="ACGE36_002586"
     CDS             complement(2045..2977)
                     /locus_tag="ACGE36_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8384934.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          934 a          676 c          568 g         1144 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ct
//
LOCUS       DAXWLH010000212         3316 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXWLH010000212 DAXWLH010000000
VERSION     DAXWLH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.218"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..202)
                     /locus_tag="ACGE36_002587"
     CDS             complement(<1..202)
                     /locus_tag="ACGE36_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384935.1"
                     /translation="MKNFDTYYVIVFSISLLLILLTLMQYKEYPEYFILTFVYLVSSY
                     TLLYKFRSSSRVSKVCFLISLLI"
     gene            complement(216..446)
                     /locus_tag="ACGE36_002588"
     CDS             complement(216..446)
                     /locus_tag="ACGE36_002588"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFF8384936.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(540..878)
                     /locus_tag="ACGE36_002589"
     CDS             complement(540..878)
                     /locus_tag="ACGE36_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384937.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1806..1922)
                     /locus_tag="ACGE36_002590"
     CDS             complement(1806..1922)
                     /locus_tag="ACGE36_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8384938.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2398..3277)
                     /locus_tag="ACGE36_002591"
                     /pseudo
     CDS             complement(2398..3277)
                     /locus_tag="ACGE36_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
BASE COUNT         1063 a          601 c          485 g         1167 t
ORIGIN      
        1 gaatcaataa gctaattaag aaacaaactt tagaaactct agaagaactt ctgaatttat
       61 atagaagtgt gtagctactt actaaatata caaaagttaa aataaaatac tctggatact
      121 ctttatactg cattaaagtt agtaatatta acagcaaaga tatcgaaaaa acaataacat
      181 aatatgtgtc aaaatttttc ataataagaa ctcccttact gttgcatact aacataatcc
      241 aaaaactctt ctacttcagc taaagacatt ccactttctt tagtccaaaa aacagttttt
      301 tctatcgatc cttctctgta aactgtaaat gtcggagtcg agttaattga gtagcgactc
      361 ttgaattctt tccactcatt ttcatcttct ctaacttttg ctacattcac gtaataaata
      421 ttttcttttc ccttttctac cagcatatcg aatagcttcc tgcttttgat gatcgatttc
      481 ttgtgctaca tctagtaaat tttcatcaat ttctatatca taaagtattt cactagtgtc
      541 tacatccaca atatatactt tttatcagta gcaatttttg agtaatccgt taaaatgatc
      601 tcagtctcgt cactttcatc agtataaata tacgttaaca atgaactcac cttttcaatc
      661 ggtgttagct ctaagcctac tgtatttttt actattgagt cattatttac caccataact
      721 tcgtactgta tataccatcc tatttggttt ccatcaatat cagtaattgg ctcactagct
      781 aatggagtct ccggaacaat attttcctga tatttcttaa actcttctgt agcacgaaaa
      841 aggttatcat gttcctcact tgaaactggt tgagtcatag aaaatgttac ttggctaaac
      901 agagggaaaa taaacactac aatcaacaaa gtataaaaaa aagcccttct ttttcatttt
      961 ttatcttcca atcacaaatt gaaaaactta ttcctgcaac tttgagtcta gtatcacaat
     1021 cgactgacta caactattca tttacattct actttaagtt cctccttaac cattaatttt
     1081 attaaagata cttgtaatag tgattctcac aaaatttcat gaaaaatact ttattactgt
     1141 atctaaaatt ccaatacaca acttatcgac tattatctga aaaccaaaac ttattattcc
     1201 ttgatgttat tctagcaata gtaactgctt ttttattttt ttgcatattt tttcggcgtt
     1261 actttttaaa gttaactttt tcgacaaata gaaagagata cgaatcactg atttttttga
     1321 agaaaagaaa tggagtgagc tgaacgttag agatattggg agctttcgta actgcttatt
     1381 aaacgataat ggttatttgc aaccgtattg ctcgcttcta catgaaatat ttcatgaaat
     1441 atttcgcaaa acctttgatt atgtagttac gtttccattc ttgactaaaa atgtttcaaa
     1501 aacgaacaaa ggtgttaaaa ggtaaagtga tcaatactac tggtcaaaat aagaggttga
     1561 aagggaattg aaaaataact acagataata cttttataaa cgtacgtgct ttgtggttct
     1621 gtagttaact atataactgg aattagggaa tggtatcaat ttgaaaagaa agattctacc
     1681 aatttatcat atgttaaaaa tggaaaatca acagcagttc tttcggaaac cttacacaaa
     1741 aaccgattat tgaaaatagc cgaaaaacgt ttttcggcta tttttttaat taagtttcct
     1801 tcttcttacc ttttcccact attccgtttc acatctttga tgatcttcag cgcacgtttt
     1861 cgtgttttga tatttgggct tttcaagcgg cggtatgcac ttgagatatc tgttttttcc
     1921 atatggacga cccactttct tctttgattg tagtcatgct aaaaaacaca aatcgaaaca
     1981 attacgattt gtgttttaaa tggtatcaaa agttaaaatg aaagtcaact tgttaaaaca
     2041 gtcgatcaga ctagctgatt gactcctatc tctggaaata aattttcatt attacgtttt
     2101 agctacctaa aaaaggccct cttgtccgtt aactttaata tctattgtta tagctagttt
     2161 acctattgaa cgtactgtgt gttcgataga acactaaaaa agaggctggg acagaagcgt
     2221 ttaactccaa gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc
     2281 agcttatttc cgaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga
     2341 caaaagtgtt ctttactttt gtctcacgct cttttaaact atttcgaagt actaaactta
     2401 tgaagtaaga atacctaagc cagagccaat gtgctcgcct gcaagcaatt gctcttttgc
     2461 ttgtcgctgt aaactcgtac tttctggata atcatgtatt tgggcatcta ctcgtccaaa
     2521 gatccaaggg actccactat cgtcctgttc atcaactgat aataatcgat aatcggctgc
     2581 aaaaagacag atttttgact cttttttcaa ccatttagta agattgggtg atagtaacca
     2641 agtatctgtg tacatcactt ggtaatggta atcaggaaaa taaagttcaa aaaaacgaat
     2701 ggcactttcg taactatctc ttatcttatt ctgagataaa tctgcatcgg aaggaatatg
     2761 aagagaaagg cactgctttc cttctaatct tagatccgtg taccctttcg gaaaatatgt
     2821 catttcgtac tccaattcac caatccgaaa gatcaagcca ctagtgtaat gccaaatcca
     2881 aaaaccacgg tcaaagcgat agcttcctgt tacttggaaa tgttcatgaa taaatctagt
     2941 aaaagcattc attgttgcca aatagatagt tcttggaatc ttcaatggtt gataaatttt
     3001 tttccaactt tgagaagctg catatagata aatagctaat gctgcaaaag gttcctcttc
     3061 atagactttg tctattttct ttgcagtcgc ctttggcttt attaggatcc attagttcat
     3121 gactatattg ctcaaacacc tcatcaagga tgttttttgc ttgtacaatc atttgctcta
     3181 taacgacttt gggaagagtt aaagtttctg ccagaatttg gatttcattg cttattttct
     3241 tcatcaaatt tccccttttc atatctttga acctcattgt ttaaatgact gattatccgg
     3301 acaccatatg gtataa
//
LOCUS       DAXWLH010000213         3281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXWLH010000213 DAXWLH010000000
VERSION     DAXWLH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.219"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..359
                     /locus_tag="ACGE36_002592"
     CDS             <1..359
                     /locus_tag="ACGE36_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8384939.1"
                     /translation="SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGA
                     GMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVI
                     SWLVTLNVYKHSQRDF"
     gene            923..2185
                     /gene="mobV"
                     /locus_tag="ACGE36_002593"
     CDS             923..2185
                     /gene="mobV"
                     /locus_tag="ACGE36_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFF8384940.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            2430..>3281
                     /locus_tag="ACGE36_002594"
     CDS             2430..>3281
                     /locus_tag="ACGE36_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFF8384941.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1208 a          436 c          676 g          961 t
ORIGIN      
        1 cttcctttct tttagaaaca acatcatggt tcatgacaat tataatcgta tttgttttag
       61 gtgggctttc gttcaccaaa acagttatat caacaattgt ttcaagtagc ttgaaacagc
      121 aggaagctgg tgctggaatg agtttgctta actttaccag ctttttatca gagggaacag
      181 gtattgcaat tgtaggtggt ttattatcca tacccttact tgatcaaagg ttgttaccta
      241 tggaagttga tcagtcaact tatctgtata gtaatttgtt attacttttt tcaggaatca
      301 ttgtcattag ttggctggtt accttgaatg tatataaaca ttctcaaagg gatttctaaa
      361 tcgttaaggg atcaactttg ggagagagtt caaaattgat ccttttttta taacaggaat
      421 tcaaatcttt ttgttccatt aaagggcgcg attgctgaat aaaagatacg agagacctct
      481 cttgtatctt ttttattttg agtggttttg tccgttacac tagaaaaccg aaagacaata
      541 aaaattttat tcttgctgag tctggctttc ggtaagctag acaaaacgga caaaataaaa
      601 attggcaagg gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa tactacctgt
      661 cccttgctga tttttaaacg agcacgagag caaaaccccc ctttgctgag gtggcagagg
      721 gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt
      781 tttggtcggc actgccgaca gcctcgcaga gcacacactt tatgaatata aagtatagtg
      841 tgttatactt tacttggaag tggttgccgg aaagagcgaa aatgcctcac atttgtgcca
      901 cctaaaaagg agcgatttac atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc
      961 agctggacta aaaggcatgc aatttcataa tcaaagagag cgaaaaagta gaacgaatga
     1021 tgatattgac catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata aaaatattga
     1081 ttacaacgaa cgtgtcaaag aaattattga atcacaaaaa acaggtacaa gaaaaacgag
     1141 gaaagatgct gttcttgtaa atgagttgct agtaacatct gaccgagatt tttttgagca
     1201 actggatcca ggagaacaaa aacgattttt tgaggaaagt tataaattat tttccgaacg
     1261 atatggcaag caaaatattg cttatgcaac agttcataat gatgagcaaa cccctcacat
     1321 gcatttaggt gttgtgccta tgcgtgatgg aaaactgcaa ggaaaaaatg tgtttaatcg
     1381 tcaagaactg ttatggctac aagataaatt ccccgagcat atgaaaaaac agggttttga
     1441 gttgaagcgt ggtgaacgtg gctctgaccg taaacatatt gagacagcta aatttaaaaa
     1501 acaaactttg gaaaaagaga ttgattttct agaaaaaaat ttagcagtta aaaaagatga
     1561 atggactgct tatagcgata aagttaaatc agatttagaa gtaccagcga aacgacacat
     1621 gaaaagtgtt gaagtgccaa cgggtgaaaa gtccatgttt ggtttgggaa aagaaataat
     1681 gaaaacagaa aagaaaccaa ccaaaaatgt tgttatatcg gagcgtgatt ataaaaactt
     1741 agtgactgct gcgagagata acgataggtt aaaacagcat gttagaaatc tcatgagtac
     1801 tgatatggcg agagaatata aaaaattaag taaagaacat gggcaagtta aagaaaaata
     1861 tagtggtctt gtagagcgat ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga
     1921 aaacaagtct ttaaagtcta aaataagcga tttaaagcgt gatgtgagtt taatctatga
     1981 aagcactaag gaattcctta aggaacgtac agacggctta aaagccttta aaaacgtttt
     2041 taaggggttt gtagacaagg taaaggataa aacagcacaa ttccaagaaa aacacgattt
     2101 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
     2161 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
     2221 atggttttga acttgttctt tcttatcttg atatatatag aaataacgtc atttttattt
     2281 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
     2341 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgacc aaacaaataa
     2401 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
     2461 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
     2521 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
     2581 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
     2641 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
     2701 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
     2761 caacagttcg ttggttgttt ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat
     2821 taaataagag tttgtcagat atggctcaaa gatttcgccg aatgatgcaa tataaaaaaa
     2881 ttaataaaaa tcttgttggt tttatgcgtg caacggaagt gacaataaat aataaagata
     2941 attcttataa tcagcacatg catgtattgg tatgtgtgga accaacttat tttaagaata
     3001 cagaaaacta cgtgaatcaa aaacaatgga ttcaattttg gaaaaaggca atgaaattag
     3061 actatgatcc aaatgtaaaa gttcaaatga ttcgaccgaa aaataaatat aaatcggata
     3121 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
     3181 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
     3241 ggttaatctc ctatggtggt ttgttaaaag aaatacataa a
//
LOCUS       DAXWLH010000214         3276 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXWLH010000214 DAXWLH010000000
VERSION     DAXWLH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.220"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            22..678
                     /locus_tag="ACGE36_002595"
     CDS             22..678
                     /locus_tag="ACGE36_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384942.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            678..2054
                     /locus_tag="ACGE36_002596"
     CDS             678..2054
                     /locus_tag="ACGE36_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384943.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2268..2597
                     /locus_tag="ACGE36_002597"
     CDS             2268..2597
                     /locus_tag="ACGE36_002597"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8384944.1"
                     /translation="MQDVLKDVFGPLFEKMLQGELNNHLGYDAHSKEPKKHDNRRNGY
                     GNKTLKTSFGKVNIDVPRDREASFEPELIPNRKRDVSDIADCRQKLFQTRNSSLEQLF
                     IYFVSNF"
     gene            complement(2520..3276)
                     /locus_tag="ACGE36_002598"
                     /pseudo
     CDS             complement(2520..3276)
                     /locus_tag="ACGE36_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
BASE COUNT         1213 a          455 c          596 g         1012 t
ORIGIN      
        1 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
       61 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
      121 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
      181 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
      241 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
      301 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
      361 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
      421 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
      481 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
      541 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
      601 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
      661 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
      721 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
      781 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
      841 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
      901 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
      961 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     1021 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     1081 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     1141 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     1201 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     1261 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     1321 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     1381 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     1441 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     1501 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     1561 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     1621 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     1681 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     1741 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     1801 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     1861 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     1921 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     1981 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     2041 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     2101 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     2161 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     2221 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     2281 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     2341 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     2401 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     2461 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     2521 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     2581 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     2641 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     2701 cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa tattcgttca
     2761 atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg aagatgttcc
     2821 acttcatcca tcagatctcc ccaaacatgt ctcgtgatga ctcgttgatg atttttactt
     2881 atggtacatt ggtttagcag aggacagttc acacattctt ttggattgga tttgtattct
     2941 cggtaccctt ctcgagtaac cgttgaaaaa gcgagcattt gatcttgcgg acaaatataa
     3001 caatcaaaat actcgtcata gagaaactca ttttttgata atagctcctt tttccctttg
     3061 ggccttgaat aaggcaaaca aggtcgaagg ttcaaatgag tcaaaaagtg aacaaactta
     3121 ggtgtcttat acccagcatc agcgacaaca caattgattt caggaaaaag tctcttcgat
     3181 tgtttgacga gctgaaaggc gacttgactg tcatgaatat ttctaggagt gatgattgtg
     3241 ctaagaataa atccattgtc gtcgcatgaa gtatgg
//
LOCUS       DAXWLH010000215         3240 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXWLH010000215 DAXWLH010000000
VERSION     DAXWLH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.221"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            120..986
                     /locus_tag="ACGE36_002599"
     CDS             120..986
                     /locus_tag="ACGE36_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384945.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWEKVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYEPTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            1141..2967
                     /locus_tag="ACGE36_002600"
     CDS             1141..2967
                     /locus_tag="ACGE36_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFF8384946.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSIPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKVCENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            2975..>3240
                     /locus_tag="ACGE36_002601"
     CDS             2975..>3240
                     /locus_tag="ACGE36_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384947.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEEL"
BASE COUNT         1295 a          383 c          626 g          936 t
ORIGIN      
        1 ttctttgttt tttgctatat ttttctacag gcaattatct taagagtatc tgtgtattgg
       61 aataaaaata tgataatatg aaattatgta tcagaccgat aaaaaagagg tgcttataaa
      121 tgaaaaagtt aagagttatt gcaacaatac ctatgatttt tagttttgcg ctattattat
      181 ctgcatgtgg taaagaagaa gctggtgcta agtcaaatga agcacataat atctcattca
      241 tagatagtgc agaacaaaaa gattcacgtt gttggttggt agtcgaaaca agtagtaaaa
      301 ataaaacgcc tattgataag aacgaatatg taaaagcggt gatccaaact aaagatggca
      361 aagcaaaagt atttaaaacg gatatggaac agcttacatt ggaaaaagtt tctaaaatga
      421 gtgatgaaga aataatagaa caagcattga acgctgataa acaaaagttt aatgataaga
      481 aaaaaagcga tattgatagc tacaatgcta ctttagattc tgtaaaaaca gaattagaaa
      541 atgaggaaaa acaagagttt caaaatgacc aaaaaattgc tgattttcaa gatagtatac
      601 ctaaaataca ggcggatata gaagagatcg atggtgttca atattcagaa ccggagtggg
      661 aaaaggtatc agcaacaatt aaaatggata atgaaaacaa atcaccttca atggaaaccg
      721 ttaaattatc ttataaatat ttagatagtc caatttggaa tttaaatagt tttggtgaag
      781 tttcgtctga aggagaatta tcttatgaac ctactagtat ggatttgaag ttgtcaattc
      841 cagttgccac aacgaaagtt ttagataatt tttattcggg atattctaat gatgaattgg
      901 atcaactttt gcttactaaa gtagcttcta aagataacaa agttcaatta gatagtaaag
      961 gtaaaaacgt aagtatagtt gagtagatgg taaaataaga agggataaga tggttactat
     1021 aactatctta tctctttttt atttgcatta agtcagttaa attagataga agagttttta
     1081 tatatctggt ataatttaat taggtaattt aacttaccta aaaaagaaag gagatagaag
     1141 atggcctata aaaaaggaaa ctggaaaccg aaaacgaaag agcaaaaaca aaaagagcta
     1201 gacgatttaa ttaaggcttc caacagcaaa atcgagcaat atcagtctaa ccctgaagat
     1261 atgatcgagt ttgccaagtt catgtctaaa atacacaatt acagtccttt aaacctatcg
     1321 ttgatcgatg agggatttga aggagcaatt gctgtagcaa gttatgatgg ttggaaaaaa
     1381 ctaggatttc aagttaaagc gaaagaaaaa gggataggca tttatgcgca tgctccggta
     1441 acggttttta ttgatgaaaa tggtgaggaa aagacgttaa aagaagcgac aagcgagcaa
     1501 aaagaaaaaa tcaaaaatgg agaattaaga agcaaaaaga tcaagcattt taagaagggt
     1561 tacgtatttg atatttccca aactaatgca actgaagctg atttgccaaa aatatttcct
     1621 aacagggtat ggaattttaa tattgatggt gaattaagtg ttagtcaatt agaaaaaggt
     1681 gtaaatcatt tggccgaatc tatgaatata tctataaaag atatgaatga gtctggtaga
     1741 ggtgaaatgg gaagcgctag aggtgaatat attcaatatg ttgatggtag ggaagaaatt
     1801 gtattaaatt caagaaatag cagaacacaa aatcttacta ccagtattca cgaattggct
     1861 cacaaaaagc ttcacaataa gaatgaacac ggttcttcct actcaatacc gattaaagag
     1921 tttcaagcgg aaatgacttc atttattgtt tgtcacaatt acggtattga tacgtcagac
     1981 tttactattc cttatattgc aaattggact aaaaataatg aaaaaattga tccaaaaaac
     2041 tttagacaga taatgcaaga agttagagaa acgtctattg attttataag tacgattgat
     2101 aagaaagtct tggaagaaca gcaaacagca tttaatgcac taaaaaattc ctttgttcct
     2161 aatgagaata aagagcagat acaacaaaaa tcagatttag aatttcataa tttctacgat
     2221 agtacaatta acgatccgga tagaacaatc cacggtagtt atttagaagc gatggtggat
     2281 tattatagag gaaagtatgg agaaaattct attgagctta ctcatagcaa ttatgatgtt
     2341 acagctaaaa taggagaaaa ctatgtgaca tattattcag atacgaaaca atttgatgta
     2401 tgtgatgttt atggtgtagt ttttgagaat ccaagtgaaa ataaaaatga tcgtttatta
     2461 ttagatcgat cacctgaaaa ttgtgattat gttgaaaagt ttgttgtaga aagtttgaaa
     2521 gtttgcgaga atcatgaact aaaaaatgat acgttaaaat taagcgaacc gctaagatca
     2581 gttgttataa atgaagcggt ttataatcta tctgaaaatg ctaacaatta ctataatgaa
     2641 aaatgttggc aagaagaaat tgaaaaccaa acaaatatta atgatgtaaa agaattaaac
     2701 tcttgtatga ctgcggaaga tttttacgaa tctcataaac aagaaatcga aaatacgatt
     2761 acagattttg aatctgtgtc aggaacaaat ttaagagata caatagacaa tgagtcatat
     2821 aaagaggtgg cttcagtggt agcttataaa ttggttgcaa ctaaagtaaa atatgaaatg
     2881 gatgaaggaa ctttcaagat accagaaaat tctaacttgg aaaagccgac tgtaaaaaca
     2941 gcagaacgtt cttttgaaat ggagtgaaaa aattatgagt aatgaacaaa attatgaaac
     3001 aggcggaact tatcgcctgt ttcatggaag tatgaataaa tttgatgaat ttagtttaga
     3061 tcataaaggt ttaaacggaa cagctaatgg atttggtgtg tacttaacac ctaataaaga
     3121 aatggctaca atgtacgctg aacagggtag taacggaaat aatggtttcg tatatgaagt
     3181 aaatgttgaa ttagagaagc cgatttctat gaatgaacgc actattactg atgaagagct
//
LOCUS       DAXWLH010000216         3238 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXWLH010000216 DAXWLH010000000
VERSION     DAXWLH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.222"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE36_002602"
     CDS             complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE36_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFF8384948.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE36_002603"
     CDS             complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE36_002603"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFF8384949.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT          879 a          635 c          597 g         1127 t
ORIGIN      
        1 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
       61 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
      121 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
      181 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
      241 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
      301 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
      361 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
      421 gatttgttct tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt
      481 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
      541 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
      601 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
      661 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
      721 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
      781 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
      841 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
      901 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
      961 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
     1021 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
     1081 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
     1141 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
     1201 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
     1261 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
     1321 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
     1381 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
     1441 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
     1501 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
     1561 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
     1621 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
     1681 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
     1741 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
     1801 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
     1861 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
     1921 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
     1981 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
     2041 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
     2101 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
     2161 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
     2221 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
     2281 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
     2341 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
     2401 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
     2461 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
     2521 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
     2581 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
     2641 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
     2701 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
     2761 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
     2821 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
     2881 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
     2941 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
     3001 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
     3061 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
     3121 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
     3181 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataa
//
LOCUS       DAXWLH010000217         3190 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXWLH010000217 DAXWLH010000000
VERSION     DAXWLH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.223"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..192
                     /locus_tag="ACGE36_002604"
     CDS             <1..192
                     /locus_tag="ACGE36_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384950.1"
                     /translation="KKWSRNQYLKVLIENDFDHALLNYKKDQFDRLLEKFVDIQTYNT
                     KTLEEYIATNNQLIGLLIE"
     gene            234..656
                     /locus_tag="ACGE36_002605"
     CDS             234..656
                     /locus_tag="ACGE36_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384951.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            675..2267
                     /gene="mobP2"
                     /locus_tag="ACGE36_002606"
     CDS             675..2267
                     /gene="mobP2"
                     /locus_tag="ACGE36_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8384952.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2279..2614
                     /locus_tag="ACGE36_002607"
     CDS             2279..2614
                     /locus_tag="ACGE36_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384953.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2761..2955
                     /locus_tag="ACGE36_002608"
     CDS             2761..2955
                     /locus_tag="ACGE36_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384954.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2987..>3190
                     /locus_tag="ACGE36_002609"
     CDS             2987..>3190
                     /locus_tag="ACGE36_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384955.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQS"
BASE COUNT         1310 a          462 c          613 g          805 t
ORIGIN      
        1 aaaaaatgga gtcgaaatca atacctaaaa gttttgatcg aaaatgattt tgatcacgct
       61 cttctgaatt ataaaaaaga ccaatttgat cggttgttag aaaaatttgt ggatattcaa
      121 acttataata cgaaaacttt agaagaatat attgcaacaa ataatcaatt aattggtttg
      181 ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca attttggcaa
      241 aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt gatgaaataa
      301 tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg tgtatgatgt
      361 atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat gcaatgggaa
      421 aagaaatttc tattttaacc gaaatggttt cagagtttgc ggatcttcat ttgaaagata
      481 caggaatact aattggtaca aattcaactg tttatcaaga agcagaagaa cgtgtggaag
      541 gaaaaattaa atccaatcaa accaaaaaat attcttctaa gttaaagaaa ccagtgaatg
      601 aatctgttca taccagagta gatccatttg atccaacgaa gattctaaaa agataaaagt
      661 agaaggagaa cgtgatgaat aaatcgcctg cagttattct tacttgtcaa tttaccttgc
      721 ccaataaaaa atctttttca acatacattg attatatgac aagagaaaaa gcactggaag
      781 aaatggaacg acgtacgccc gaagaagaac aggaactgca acgaatcaag aatgctttag
      841 aggattttta tattcctgaa ggagaaactt tttcggaaag taagacggat aaagaattca
      901 gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt gacaagttag
      961 aggaacaaga ttttaccaaa tatctttctt atatgacccg tcactatgcg ttagaacaaa
     1021 agaaagaact gacgcaaagg gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa
     1081 aatatcaaac agagccacat aaaaaagctt caggaaatct tcctggagtt ttttctatgg
     1141 cttcagacga agtgaaagga agtgatctga aggagatcaa acaaatcttc caaagaggac
     1201 aacaaaaagg ctcaatcatt tatcaagatg ttatctctca cgacaattcg tatcttgaaa
     1261 aaatgggctt gtacgatccc aaaacagata ccttagatga agaaggtttg aaagcagctg
     1321 gaagaaaaat gatggagaca ttattcgaga aagaacaatt gaatgaaaca ggttactgga
     1381 tggcaacgat tcaccgtaac actaagcata tccatattca ttttgcagtt gttgaaaaag
     1441 aaaatacgcg taaaccgtat actgagattg aaaatggtgt gagttatatc gtaccgaagg
     1501 ggaagagaag acaagcaaca ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac
     1561 gtttctctta tgaaaaggat cgaatgatcg gtatggagaa acgaaattta ttgacacgaa
     1621 aaagtgattt gcgtaacacc ttaacaaaag tagtaaaaga accagcacgt tacgatcgac
     1681 acgccatgaa cttattaaat gaagtatata aaaccctacc tgaaaaacgt tcggaatgga
     1741 attatggaga tgaaaatcga accaagcttt caccatttac aaggggaaaa cttgatgagt
     1801 taaccaaata tgttttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     1861 cattgaaaga agaagccaaa agcctttatg gggaaagcaa aagagaaaca aagaatgcag
     1921 aaaaaaacgc cctctttgat ttaaaaaagc gcacaggaaa tgcgatttta acagaactga
     1981 agagacagga tagtcaaata aaaccattaa tagaaagtgt caatcagatg caatctattt
     2041 acaatgaacg atcctttcga acactcaaac agttgccaac aaatgaatac aagtatagtt
     2101 atcaagaatt cgtaaagcgt aggaaacaat tccaacaaag agttgtcacg cagcgaagct
     2161 tgcgtcaatt aaatcgaaca attaatgcac atgaagaaaa ataccacgca agtaaagcat
     2221 atgaagagca acagcgaaga atccaaatgg aacaagaaag acaataggag gaagagaaat
     2281 gaaacaatat ttagatcaat ggaaagtcat agaaggatca ctaaaagaag aacgaatcga
     2341 acaacttcct gattgtttag aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa
     2401 cgaacagttt gatccaaatc aatttttagt tgttgagtac ccagctactg gtgtgtattg
     2461 ttgtaatcat gtgaagggtg aaaaatattt tattattcaa gaatacgaag gtaagttagc
     2521 gccttattat actacttggg aaataaatga agagggaatc aataatttcc catgtgaaag
     2581 cattgaagag tctatttctc tcacggaatg ttaaataaat agagacgtaa aagagtcctg
     2641 aacgtgattg tccaggactc tttttcaatg aattgttttc cgccaattca ctgagtggtg
     2701 cgattcaatt ttattatatc aaaagaaagt gagattccaa agtgaaggga ggaagaagaa
     2761 atgactcaga caacgattgg tgttgtggtt gaaaaaagca gggataatct cgtttttgtg
     2821 acagaaattc aaacaggtag agcttttgta gtcacagaca aagctgcaaa agcgtatcaa
     2881 aatggcaata ttttaacgtt aaatctgact acgaagatgt tcgttgatgc agtagaaaat
     2941 tatccatttg tttagacaaa cgatatttta caaggaggaa acggacatgg cagaagaaaa
     3001 agaaccacta gataatagtc ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa
     3061 cgaattaaat aatcaatttc aaaagctatt tgatcaccaa aaattgacaa atgggcaacc
     3121 aatgaatgac aaacgaaatg gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa
     3181 aatacaaagt
//
LOCUS       DAXWLH010000218         3178 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXWLH010000218 DAXWLH010000000
VERSION     DAXWLH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.224"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            35..1228
                     /locus_tag="ACGE36_002610"
     CDS             35..1228
                     /locus_tag="ACGE36_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8384956.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1221..2948
                     /locus_tag="ACGE36_002611"
     CDS             1221..2948
                     /locus_tag="ACGE36_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFF8384957.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         1068 a          546 c          722 g          842 t
ORIGIN      
        1 gaaaaatgaa tttacataaa aaaggagaat ttgtttggta aaagtagaag taaaacattt
       61 aacaaagata tttggtaaaa agacacaagc cgctttagat atgatgaatg atcaccagcc
      121 gaaaacggag attttaaaga aaaccggtgc aacggttggt gtgtatgacg tgaattttga
      181 cgtaaaagaa ggagaaatct tcgtgatcat ggggctttcc gggagtggga aatccacatt
      241 gatccgtcta ttgaatcgtc tgattgagcc aacttcgggc agtatctata ttgatggaga
      301 agatgtatcg aagttaagca aagaagaatt gcgagaagtc cgtcgtcata aaatcaatat
      361 ggttttccaa aacttcggtc tgtttccaca tcgaacgatc ttggaaaata cagaatacgg
      421 gttagaagtc cgtggtgtac ctaaagaaga acgacaagaa aaagcagaaa aagcattaga
      481 aaattctagt ttgctttcat ttaaagacca atatcctagt cagttatctg gtgggatgca
      541 acaacgggtt ggcctagcca gagctttagc aaatgatcct gagatcttgc tgatggacga
      601 agcgttctcg gctcttgatc cgctgatccg gcgagaaatg caagacgaat tactggacct
      661 gcaagccaat gttcaaaaaa cgattatttt cattacccat gatttgaatg aagctttacg
      721 gattggggac agaatcgcct tgatgaaaga cggcgaaatc atgcagatcg gaacaggtga
      781 agaaatattg accaatccag caaatgatta tgtacgtgaa tttgtggaag aagtcgatcg
      841 ttccaaagtg cttacggctc aaaacatcat ggtacctgca ttgacgacga atatcgaatc
      901 tgatggacca aacgttgcat tgacaaggat gcgtaatgaa gaagtcagca tgctgatggc
      961 tgttgacaga aagagacacc tgaaagggat catcactgca gatcaagcgt tagaagctag
     1021 gaagcaaaaa cgtccattga tcgacttcct agatgaaaat gtgacggtga ttgggaaaga
     1081 catggtggta agtgatattt tcaatattat ttatgactca ccgactccac ttgcagttgt
     1141 agaggatgga aaattaaaag gtgtcgtcat ccgaggtagt gtgattgaag cattagcaga
     1201 aacaagcgag gtgagtgaac atgaataatc tattgaacta tcaattacca gtggcaaact
     1261 gggtagaaaa tatcacagaa tggtttacga caactttttc tggcctattt tcatttttac
     1321 aaacgattgg acaggcagtg atgtcgggga ttacaaattt actgttagtg attccagctc
     1381 ctttgtttat cttgttattg acggtagcag ctttctttat ttctaagaaa cgtccaggac
     1441 ttacgttgtt cacattaatc ggtctatggt ttatttataa ccaaggtcta tggaatgatt
     1501 tgatgaatac tgtcacactt gttcttttat ctagtgtgat ctcgatcatc attggggtac
     1561 cattagggat cttgatggca aaaagcagta aagcccaaag tattatcaaa ccgatccttg
     1621 actttatgca gacaatgcca ggattcgttt atttgattcc agcagttgca tttttcggta
     1681 tcgggatggt tccaggggta tttgcctcag tcatctttgc tctgccacca accgttcgtt
     1741 ttacgaattt agggattcga caagttccta aagaattagt agaagcttct gattcattcg
     1801 ggagcacaag tcgtcaaaaa ctattcaaac tagaattgcc tttagctaaa agtacgatca
     1861 tggcagggat caatcaaaca acaatgctgt cactttcaat ggtcgttatt gcctctatga
     1921 tcggtgcacc cggcttagga cgcggcgtat tatcagcatt gcaaagagca caagtcggga
     1981 atggatttgt caacggggtt gcattagtta tcttagcaat catcattgac cgttttacac
     2041 agcatttgaa ccagccaaac aataaaaaag ctgcaggagc aacaacacag aaaagtaaaa
     2101 aacgtcaagg actgatcatt ggtgctgtag tagtagtgat cttaggtgcg attggtatag
     2161 ggagtttttc atctgccaaa gaaacaaaac gtatcaactt atcttatgtt gaatgggata
     2221 cagaagttgc ttcaacaaat gtagtcggtg aagtattgaa acaaatgggc tatgatgtaa
     2281 cgatgacgcc attagataat tcaatcatgt ggaaatctgt ttcaaatggc gagtcagatg
     2341 ccatggtttc agcttggcta cctaaaactc atggttcaca atatgcacaa tataaagatc
     2401 aagtcgaaga cctaggtgcc aatttgacag gagcaaaagt tggtttagca gtgccagcct
     2461 acatggatgt aaattcgatc gatgagttaa ccgatcaggc aggtaagaaa atcataggaa
     2521 tcgagccagg agctggtgta gttacggctg ctgaaaacac gatccaaaaa tatgacaact
     2581 tgaaagactg gaaagtagag acatcttctt caggagcaat gaccgttgca ctaggtcaag
     2641 cgatcaaaaa acacgaacca attgtagtta caggatggac acctcattgg atgtttgcca
     2701 aatatgatct gaaatactta gaagatcctg aaaacggaat gggaagcgaa gagcagatcc
     2761 atacgatggt tcgaaaagga ttgaaagaag accagccaga agcttacaaa gtattagaca
     2821 atttccattg gtcagaaaaa gatatggaaa aagttatgtt ggaaatcaac aatggaaaag
     2881 atccacagca agcagcaaaa gactggatca aagagaacca agaattagtc gaaagctgga
     2941 aaaaataaaa agacagacac gaaataaatg gagcttccaa aattggatca aatatccaaa
     3001 tttggaagct ccatgttcgt ctctatcttt tttgtctata aaaaaagaga atgcgtagct
     3061 catcttcgaa acgttcaaag acgccgacaa actattatga atttatgggt gcgagtcaaa
     3121 gagatcgatc gtaggtatga aagactaatg tgattaaagt ttattatgta cgaagtgc
//
LOCUS       DAXWLH010000219         3134 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXWLH010000219 DAXWLH010000000
VERSION     DAXWLH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.225"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>3134
                     /locus_tag="ACGE36_002612"
     CDS             <1..>3134
                     /locus_tag="ACGE36_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFF8384958.1"
                     /translation="IKSNTQEVDKDATVLSDTPRDLVNLEDTSTDIENVTPQKIGKKD
                     STSLSEENLSSPASSILRRRTRSLDVQTGETFLEDWALPDNSNISGSLITQTGITYVG
                     VKTDDPTQLHLKMAYRAAATPAAIDWIYIRVDPELTKYIEEINIKNTVNIGAQVGYQR
                     YFEKVDANTLSDVNTFGTPTGVSDAYAQVDQTTGGIYRVLLDKFFTRIPGVKPKERGA
                     LSGNWGVFPSVPLEAERYEANIYIKLSKDVAELQQELGRDKFGVQLRLQGQSSSGVAT
                     IAKESLNKNTIIDLSKQESIENNSNWLNSPSVHTITTTYEGKQMGSDGQFSNTLEGTD
                     KSLIRVQNTIMNNLLYGTALDLGRPVSFNIEIDPQILNLVSDDDYITIWQVYRENSIN
                     HHDGGSNAPVMLKKNMFVDNKIKIEVNKDKAGQYENGVYYVNAVESFMNTVSSSPFVG
                     ANFIDIPVMPKPLIEDEGQLVSAEIKTWFTDKKNDQERVVRNSLASSYINLFPTTINF
                     YDYSTKDTEQLLKLRESTYLVSTFGQEINGMDMGETYEISNYEFVTADPESLIITQKD
                     GNVINLYYKKLSSDAEFYEPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEG
                     VIDTTQPGEYEGTIEVTYPDGTKDTVKIPVEVVDNRSDAEKYTPEVEKEEVNYGGTID
                     LTDNVTNLEELPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVKIPVEVVD
                     NRSDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEGVIDTTQPGEY
                     EGTIEVTYPDGTKDTVKVPVEVVDNRNDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEE
                     LPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTFPDGSSTTVKVPVTVKDKVDWVEINPI
                     PTIDPEQIVPEEVHKGEEADLTDNILNLPEGSKVEVVTPVDTTQVGEHTGEVKVTFPD
                     GSSVVVKIPIIVKNMDDMITNNQMTRVEKNSVVLDVINKSKSLSSSKIFGKSDNMYVE
                     TSNKELPKTGEENTT"
BASE COUNT         1185 a          460 c          676 g          813 t
ORIGIN      
        1 ataaaaagta atactcaaga agtagataaa gatgcgacag ttttatctga tacaccaaga
       61 gatttagtta atttagagga cactagtaca gatatagaaa atgttactcc acaaaaaata
      121 ggtaaaaaag acagtacgtc tttatcagag gaaaatcttt cgtcaccagc atcaagtatt
      181 ttacgcagaa gaactaggag tttggatgtt caaactggag aaactttttt agaagattgg
      241 gcactaccag acaattctaa catttctgga agtttgatta ctcagactgg aattacttat
      301 gtgggtgtaa aaactgatga tcccactcaa ttacatttga aaatggctta cagagctgca
      361 gcaacaccag ctgcaattga ttggatttat attcgtgtgg atccagagct tacgaaatat
      421 attgaagaaa ttaacataaa aaatactgta aacatcggcg cgcaagttgg ttatcaacga
      481 tattttgaaa aagtagatgc taatacattg agtgatgtaa atactttcgg aaccccgacc
      541 ggtgtaagtg atgcttatgc acaagtagat caaacaactg gcggaatcta tcgtgtgtta
      601 ttagataaat tttttacacg tattccaggt gtaaaaccta aggagcgagg tgcgttatct
      661 ggaaactggg gtgtatttcc atcagttcct ctcgaggctg agagatatga agcaaatatc
      721 tatataaaat tatcaaaaga tgtcgcagag ttacagcaag agcttggaag ggataagttt
      781 ggtgttcaac ttcgtttaca aggtcaatca agttctggtg ttgcaactat tgctaaagaa
      841 agtttaaata aaaatactat tattgatcta tcaaaacagg agtcaattga aaataattca
      901 aattggttaa actcaccatc tgtgcataca attactacta cttatgaagg aaagcaaatg
      961 gggagcgatg gtcagttcag taatactctc gaaggaactg ataaaagtct gattagggtt
     1021 cagaatacaa tcatgaataa tcttttatat ggaacggcac ttgatttagg tcgacctgtt
     1081 tcatttaata ttgagattga tccacaaata ttaaatttag tttcagatga tgattatatt
     1141 acgatttggc aagtatacag agaaaatagt atcaaccatc atgacggtgg atcaaatgca
     1201 cctgtcatgt tgaaaaagaa tatgtttgta gataataaaa ttaaaattga agtcaacaaa
     1261 gataaagcgg gtcagtatga aaacggagtt tactatgtga atgcggttga aagttttatg
     1321 aatactgtga gttcctctcc atttgttggt gcaaacttta ttgatattcc tgttatgcct
     1381 aaacctttaa ttgaagatga aggtcaattg gtatcagcag aaattaaaac atggtttacg
     1441 gataaaaaaa atgatcaaga gagggtagta agaaactcac ttgcttcaag ttatattaat
     1501 ttattcccta ctactattaa tttctatgat tattcaacca aagatacaga acaattattg
     1561 aagttacgtg aatcaaccta tttagtttct acgtttggtc aagaaattaa tggaatggat
     1621 atgggggaaa catacgaaat tagtaattat gagtttgtga ctgccgatcc tgaaagttta
     1681 ataattacac aaaaagatgg aaatgtcatt aatttatatt ataagaaact ttctagtgat
     1741 gctgaatttt atgaaccaga agtagaaaaa gaagaagtca actatggagg aacgattgat
     1801 ctgacggata atgtaactaa tctggaagag ttgccggaag gcacgaaagt gaacgatgtt
     1861 acaccagaag gtgtcattga tacaacccag ccaggtgagt atgaaggaac gatagaagtt
     1921 acgtatccag atggaacaaa agacacagta aaaattccag tggaggtagt ggacaatcga
     1981 agtgatgctg aaaaatatac tccagaagta gaaaaagaag aagtcaacta tggaggaacg
     2041 attgatctga cggataatgt aactaatctg gaagagttgc cagaaggcac aaaagtgaac
     2101 gatgttacac cagaaggtgt cattgatacg actcaaccag gtgagtatga aggaacgata
     2161 gaagttacgt acccagatgg aacaaaagac acagtaaaaa ttccagtgga ggtagtggac
     2221 aatcgaagtg atgctgaaaa atatactcca gaagtagaaa aagaagaagt caactatgga
     2281 ggaacgattg atctgacgga taatgtaact aacttggaag agttgccgga aggcacgaaa
     2341 gtgaacgatg ttacaccaga aggtgtcatt gatacaaccc agccaggtga gtatgaagga
     2401 acgatagaag ttacgtatcc agatggaaca aaagatacag tgaaagttcc agtggaggta
     2461 gtggacaatc gaaatgatgc tgaaaaatat actccagaag tagaaaaaga agaagtcaac
     2521 tatggaggaa cgattgatct gacagataat gtaactaatc tggaagagtt gccggaaggc
     2581 acgaaagtga acgatgttac accagaaggt gtcattgata caacccagcc aggtgagtat
     2641 gaaggaacga tagaagttac cttcccagat ggatcaagta caacggttaa ggtgccagta
     2701 acagtcaaag ataaagtaga ttgggtagaa atcaatccaa ttccaacaat cgatccagaa
     2761 caaatcgtgc cggaagaagt tcataaagga gaagaagctg acctaacaga taacattctc
     2821 aaccttccag aaggaagtaa agtggaagtc gtcacaccag tggatactac acaagtaggc
     2881 gaacacacag gcgaagtgaa agttaccttc ccagatggtt caagtgtggt agtaaaaatc
     2941 ccaataattg tgaaaaatat ggatgatatg ataactaata atcaaatgac gagagtagaa
     3001 aaaaattctg tagtactcga cgttataaat aaaagtaaaa gcttatcttc atcgaagatt
     3061 tttgggaagt cagataatat gtatgtagag acaagcaata aagaacttcc taaaacagga
     3121 gaagaaaata ctac
//
LOCUS       DAXWLH010000220         3117 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXWLH010000220 DAXWLH010000000
VERSION     DAXWLH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.226"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            177..944
                     /locus_tag="ACGE36_002613"
     CDS             177..944
                     /locus_tag="ACGE36_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384959.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            934..2976
                     /locus_tag="ACGE36_002614"
     CDS             934..2976
                     /locus_tag="ACGE36_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384960.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         1010 a          494 c          621 g          992 t
ORIGIN      
        1 gaaaacttgt agctggtgga tttgtttttt gcatatttaa ttttgctgaa taaaataatt
       61 gaataaaatt ttttattcag tccaagtaaa agtgacaaaa acataagagc aaattattcg
      121 tagatatgct actatgcaaa taaatagaaa tgaaaaatga aagaggagga gaagaattgg
      181 aaaagatgat cgaagtgaaa catctaagta aaagctacgg cagcaaactt tctcccgtca
      241 aagccttaga taatatctca ttcacagtag aagaaggcga gtttgttggg attatgggcc
      301 caagtggtgc aggaaagaca acgctgatga atatcttgtc cacgatcaat ttgcctacga
      361 tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt gagctcagtg
      421 actttcgtcg aaaaaaacta ggattcattt ttcaagagtt caatttgatc gataccctaa
      481 atgcaaaaga caatatccta ttgcccctag cagttgaacg gatgactaaa gaggaaatgg
      541 aaaagagagt aagacatgta gctcaattgt tgaatataga agaactgctc aaacggtatc
      601 ccgatgaact ttctgtcgga cagagacaac gaattgcagc tgctagagca cttgttgttc
      661 agccacaagt gatttttgca gatgaaccaa ctggctcttt ggactcgaag tcggctacag
      721 aattgttgaa ctacttgaag accatgaatc aaaaagaaaa agcaacgata ctgttagtca
      781 cccatgatcc atatacagca agctactgcg atagaatttt atttattaaa gatggtgtga
      841 ttttttctga agttgtcaga agagggacaa gaagagagtt ctttgagaaa gtaatagaca
      901 tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt ataaactagc cgttaaaagt
      961 ttttttcggc agtcacgagg ctatcttgtt tattttttca gtttgacttt atccgtaatg
     1021 atctattatt ccttcagcgc aatgacctac gatcaatctt tagtcagaag agcaagtcaa
     1081 gatacttcga tagatggtgg attgagtttt ggtggattta tcattactgt tgttttgctt
     1141 tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga aataggcatc
     1201 tatcagcttt ttggtatgaa taaactgcag atatcaggta tatatgtgtt agaaatcatg
     1261 ataattggat tgttcgcctg tatttctggg atattactcg gtattatttt ttccaaactc
     1321 ttttcaatga ttttggttcg aatgatggat atggatctta ctagtccatt ttttatctcc
     1381 attccatcag tagtagatac tttatttgct tttttcatca tccttttagc agtatctgta
     1441 tattcaatat ggaaaatatg gcgctatcca atgattcgaa gttttggaga aaaagaacaa
     1501 gcagatagtt caacaatgcg cttcaggaca aggcaccgat tactcgcagt catcgggtta
     1561 ctacttttaa cgagcggata ttttggagct agtcatttta gggaaatcac ttcatggatg
     1621 atcagttatc attatcttga tacattagtt ttcttttttc cttttttaat ttttttcgtt
     1681 tgtgtggtcg gtacttattt gtttttctgt tatacatttc ggttattttt gatcatattg
     1741 agtaaatgga agatttatta ctatggtatc aattttttga taatgggtaa cacgcaagtt
     1801 catttgctca aaagttggcg tacaaattct ctgattacag tcattctcgg aatttcttta
     1861 atgacaattg gcggcacggc tagtatctct actattttat cctaccagga gaaagtttcc
     1921 tccccgatgg actatcagtt tgatgttgag acagagaaaa aaatcaagcc gattctagca
     1981 gaggaagggc agaagataac taaagaaatc accttgcatt ataaagtcat tgggagtatc
     2041 tatagtcaaa atctcgaaga acctatgttt caaactgcta atctgatcac ggaaacacaa
     2101 tatcaagcct tcagaaaagt taatccagat ttgccgaaca tttctttgaa atcacctggt
     2161 catactgttt tgcttgatca aatgcaaact ctttttcgga atatttcatt atatggatct
     2221 agtattggac tgccagataa tcaagaattg aagattcaac aaatgcttcc aagtgtatta
     2281 ggtgacgaaa caatggtata tctaagtcct gtactgattg tagatgatac cgtttttgaa
     2341 aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg aaataatgaa
     2401 aaaatagatg aacgactgag caatgacctg tcgataaagt ggctcaatgc tgtttattat
     2461 tcttatgata tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa acttccggat
     2521 gaccaaatga aggaagcaca atttagccaa gaaagctctc gtttgaattt ctcttccagc
     2581 tattcgaaat tgcgtgcagt taatcggcga atcggtatca atatttttgt cacactattt
     2641 gtcgggatga tcgtgattat tgctactggt agtattttga cagtccggca attagcagaa
     2701 gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc tggggattcc acaaagaagg
     2761 atcagacgga tgatcttcga gcaaaacgca cttaccttct ttccacccat gatattaggg
     2821 atcactcacg cagtatttgc aatcaatgtc ttcactcagt atataaaaac tgccgattac
     2881 tggttggctt atcttgtgag tggcttattg atccttatct atcttttgct ttatgtcatt
     2941 acgagtcagt tatattgtcg gattatcgag gaatgatata cggaataaga caagataata
     3001 aatagaacta aattttgaat accatttatt tggaataaga ggttgtgaca cgatttttgt
     3061 cacaacctct ttatttttta tattttttaa aagtacgaga aattataaag aaaaaag
//
LOCUS       DAXWLH010000221         3099 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXWLH010000221 DAXWLH010000000
VERSION     DAXWLH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.227"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            237..1628
                     /locus_tag="ACGE36_002615"
     CDS             237..1628
                     /locus_tag="ACGE36_002615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8384961.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1666..2496
                     /locus_tag="ACGE36_002616"
     CDS             1666..2496
                     /locus_tag="ACGE36_002616"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8384962.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2468..>3099
                     /locus_tag="ACGE36_002617"
     CDS             2468..>3099
                     /locus_tag="ACGE36_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8384963.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIF"
BASE COUNT         1075 a          437 c          608 g          979 t
ORIGIN      
        1 atatgaatat tgtaacttgt gaataaggaa ttagcaggag aatactagtt cagcataaag
       61 gaagtgatgg gacttctgag acaactttct gttatgcaga aatacacata taaacagaca
      121 ataaacgctt ttccccgttt cttttaattt cctttacaat ttggttattc actaggtttt
      181 taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt gtatatatga
      241 ataaaaatgg ggagtggaat gcggcacgca gaatattcat tattcttatg gatgtacttg
      301 tattaaatgt atcgatctat cttagttttt tgctgaaatt ccagggagaa gtaccagtga
      361 gaaacttaga aacctttcag cattcagcaa tttttatttc catcatcttt attgcgttga
      421 atattttgtt aggagcttat gtattctata accgaatgat cagtgatatt gtcttcatta
      481 ctgttattgg acaagtacta atagcattgg ggatcatggt agttactttt gctggccgat
      541 ggtttgcatt tccgcgtggc gtcattttga tctcgtttat cctaggtacg atattactta
      601 gtgtttaccg gatagtcgtt tacaaactgt acttaagagt cagtcgtgat aagaaagtcc
      661 ttatcgtggg tttggagaaa gatgtcgcac cagctatcca aaattttcaa tctaagaaaa
      721 acaataaaca taaggttgaa gcagtggcaa tcgataatta ttacgaaaat acaaaaaaat
      781 tgatcgatga tatggatatc gtctatttgg cttctaaaat cccagaagat gaaaaaatca
      841 acatttatca attagtgact aaaaaagaga agaaattatt tttaaatacg acttttgaga
      901 atctgacaat gataaatccg aatattatga attttgaaga tgaaagcatc attgaagctt
      961 ctgggttcag aatccaacca gaatacgaac tattcaagcg tctctttgat atcatcgttt
     1021 cgcttatttt actgatcctc gcctcacctt ttatgctaat cacagcgatt ttggtaaaaa
     1081 caacttcacc tggaccaatc atctataaac aagtacgtat tacgaaaaat caaaaggaat
     1141 tttcaatcta taaattccgt tccatgagtg cgacagctga agcaaaatca ggaccagtct
     1201 tggctaaaag taacgatgca cgtgtgacac cagtaggtaa atttatccgt gctgtacgtt
     1261 ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatt gtcggtccaa
     1321 ggccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat tattacttaa
     1381 gacacaatgt acgtgcggga attaccggat atgcacaagt atacggaaaa tatgtatcag
     1441 attataacag taaactacgt tttgatttgt tgtatatcaa aaagtattcc ttactattag
     1501 atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtcc tcacaaggac
     1561 tagatgaaga tgaaaatatc agttcattag cagaaagtgc atttccaaaa gaaaaaacgt
     1621 atcgttagat tggaaaaatt ttgagcaaag aagtaggaga gaacattgga aaataaaaat
     1681 ataagtatac tgctttcttt gtatgataaa gaaaagccgg aatatttaaa ggaagcatta
     1741 gctagtatct ttgagcaaac ggtgatgccg gcagaaatcg tattggttta tgatggccct
     1801 ataaatgaga agcttcaggc agttgtcact tatttccagc aaaaagttcc tcacttgttt
     1861 acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaac tggattagaa
     1921 catactaaaa atcgcttagt ggccagaatg gatacagatg acatcatgga accacacaga
     1981 ttggaaaaac aattagagat cttccaagaa tatccagaaa tagcgattgt tgggagtaat
     2041 attgaagaat ttactggcac acttagcaat gttatcggaa gaaggattgt acctgagtat
     2101 aatgatgaaa tccgtgtgtt ttcgaaaaga agaaaccctt ttaatcatat gacagtcatg
     2161 tttgataaaa atgctatttt agaagtggga aactatcgcc cattaattgg atttgaagat
     2221 tattatttgt gggtaagact attgaaggcg ggatataaag gatataatat ccaagagatt
     2281 ctagtacatg ctagagcagg tgctgacatg tatgctagac gtggaggaag taaatatctt
     2341 ttaccaggaa ttaaaggccg atatcgcgta tggaaagatg gtctaggatc actaaaagat
     2401 tttctgatag ttatttgcgg acatcttgta attagtttat taccgaataa aattagggga
     2461 aaagtatatg aatcaaaact tagaaaaaca aactaaactt tctataatca taccttttta
     2521 caatgcaaag aatactttaa gaagagctgt agacagcgtc ttaacgcaag gttatcccaa
     2581 tattgaaatc ttgttaattg atgatggatc tacagatggt agtagtgaca ttggtgacga
     2641 aattgctaat ctagagagta aagtagcatg ttatcacttg gaaaacgctg gtccatcagt
     2701 tgctaggaac tatgggataa aagaagcaac tggaaatttg gtaggatttt tagatagtga
     2761 cgattatttt ttacctgatt gttttaataa tgttttgaaa gactaccata ggaatcaccc
     2821 cgatatcatt gcttttggat taagtaaagg aaaaaatata gaaaatgcaa atttatttgt
     2881 tcccgaaaag aagattggtt gttcaagtga agaagcaatt gagcaaatgt ttagaagtaa
     2941 ggcagtggac ttttacgtat ggaataaatt ttttaaaagg gaattattca atcatatagt
     3001 ttttccagaa aataaattat atgaggatat ggttcctatg tatgaagctt ttagggcggc
     3061 tagtaaaatt gatattttgc cattttcagg tatttttta
//
LOCUS       DAXWLH010000222         3074 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXWLH010000222 DAXWLH010000000
VERSION     DAXWLH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.228"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            334..843
                     /locus_tag="ACGE36_002618"
     CDS             334..843
                     /locus_tag="ACGE36_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384964.1"
                     /translation="MKKVKREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            840..2144
                     /locus_tag="ACGE36_002619"
     CDS             840..2144
                     /locus_tag="ACGE36_002619"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8384965.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            2252..>3074
                     /locus_tag="ACGE36_002620"
     CDS             2252..>3074
                     /locus_tag="ACGE36_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFF8384966.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSD
                     AENGASETETTSNVPIDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLANLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVASGSPTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLV"
BASE COUNT         1045 a          502 c          628 g          899 t
ORIGIN      
        1 cggagtttct tgatgaaaat atgcttctat cattacaagc tcgtttggtg taagatgggt
       61 ataagtcatt tatgttcact ctccttgtat gctttagcgg gtattacaat ttgagtgtaa
      121 cataaatggc ttttttattt gtctcgctta attatacaaa cggcgagata aaaaataaaa
      181 agaacaactg ataaaaagaa caaaatattt ttcatttgct gatgagaaat gttttgttct
      241 ttttcttagg aaaaatttag gaacacttta tgtgaaaaaa atgaaatgta tggtaaactc
      301 ctacgttgta acaaaaaaat gtaaggtgag gatatgaaaa aagtaaaaag agaagaagtt
      361 gcatcaattt tggtgaaaga taagtatttt tcaaaaggta attactggtt aaagatttgg
      421 caaacgttag tggctctttt aggatggatg atcgttgttc ttccttttat atgggtcttt
      481 ttcccattaa cgagaccaga aagagccaag gatttttcct tgtacgcttt tttatcagaa
      541 aagaaaatgc tgtatttctt aatcggcttt tttgtattga tttttttcag tttcctgatt
      601 acctcatttg tattgactag gtggaacaat cgaaatctat acaaaaaagt aaataaatct
      661 tttgaatatg aagatgaaaa attgaaaaaa agacaagaat tacttgaaga agtgtatacg
      721 gaacgttttg gaacaaaaga agagagagaa acggtccgtt tttattctgt ctccgaagag
      781 aaaaatctag atacgacatt cattgcagat ttgtataaag aaaatggggt ggaactgaaa
      841 tgatctcaat cacccataca ggtaatttat tcttagatac ttgtttgtct attatgtatt
      901 tttttctcgt ttcttatccg attttaggcg gatttgtatg gtttattggt gtctggtgtt
      961 atgtgttttt atacaaacat aagcaaaaag aatgggtaga tgttcccttg agtgtcgaac
     1021 cgttcatcac gatcatggtg ccagcccata atgaagaaat tgtgatcgaa tatacaatcg
     1081 agtatttgat gacgaaattg aattatcaca actatgaggt attagtgaca gacgatggtt
     1141 caacggacca aacaccagaa atattagcta gactgatgaa aaaatacgct aatcttcgtg
     1201 tggttcggat cgaaaaaaat aaaggaaaag cccacgcgtt taatatcgga cttgcctttg
     1261 caaaagggaa attgatttta agcaatgatg cagacacggt accggagcca gatgcactga
     1321 ttaggtatgt gaattatttc attcgccctg gtgcaagaca tattgctgcg gtcactgcaa
     1381 atatggatgt ccaaaatcgt accaaattga ttgcaaaatc tcaaacggtt gaattctcaa
     1441 gtatcgtggg catcattaaa cgaacacagt cagctgtttt cggtggtctt tatgcgtaca
     1501 gtggagcaaa tacgatgtat cgaaaagaag ccttgatcga tgtgggcgga ttcagacaag
     1561 atcgagcgac agaagacatc agtattgctt gggatcatca gttaaatgac tggttatctg
     1621 tttttgctcc tgagattatc tttttcatgg aagtacctgt gacgttgaag atgctatatc
     1681 gacacagaaa acgttgggca aaaggtggaa cagaagtctg gctgacgaat ttcaaaaaag
     1741 tgatgctcca tccttttaaa catattggaa gaacgatcat cttcattgat caaacattga
     1801 gtattgtctg gtcaatattc ttttgtattt ccgtagttct ttttgctgga ttaatcgggc
     1861 attatgtata tcaaggaaac tacgaacaga tttatattac ttttacattc agttttgtgt
     1921 tcatctgctt tgaaatggtc gcaggatttt tccagttgct agcttcgctg attgtagatg
     1981 atcgacgaag aaaattgaaa taccttcttt tcgcaccact atatatgctt ttgttctgga
     2041 tagtgaacgc gataacgatc gtgacgactt tcattccagc agtcaaaacg attttaggtt
     2101 atggaagcgg gacatggaaa agcccggaac gtacaaaaaa ataaaagatg caccgttttt
     2161 tagacaaaca gcctgttgtg tttatggttc ttccaaacat ttttgcttat aataagaaat
     2221 tagttaaaaa gtagtaagga tgggaaaagt gatgaaaaaa agaatgaagc cgttaatctg
     2281 gccaattgct gtttccagta ttttcttatt gggacagaca gcttttgcgg aaacttctga
     2341 acctgctcaa acagaacctt caacaactat aagctcttct gatgctgaaa acggtgcctc
     2401 ggaaacagaa acgacttcca atgtaccgat agataattca ggagaaacga tttcatcttc
     2461 agaagataca tcaataaata gttctacctc ttctgaccaa atcgatcatt tgcaagactt
     2521 ggaaaataaa agaaaagaac tcgaagaagc attagaagct gcaaaaaaca aattgaaaga
     2581 agaccaagca gcaatcgatc aagatttagc taatcttaaa aataaagtgg gaaatgaccg
     2641 tgcacaactt gagaaaaagc ttgccgaagc gaaagcaaaa gcggaagctg tccaaacaga
     2701 tcttgacggg aagatccaac aagcaaaaga attcaatcag caattaaatc aggttgctca
     2761 aacagcttct ggaaagctaa gtcaaactca agcggatatc acagcggcca gtcaagcaat
     2821 ccacggaaag atcagcgaag cacaaacagt tgctaatgat tttgaagcaa aaaaagctga
     2881 cttagcagct agtttccaac gcataaaaga tattttgcat tcggcaggag aaaaatcagt
     2941 ggcctctgga agcccgacag aaaccgttca gcaaacgaca gagccatcca aatcaacaag
     3001 tcagactttg ccaaaaacaa atgaaaaaac agcttctgtc agttattcct tagcaggggc
     3061 gggtgtatta gtcg
//
LOCUS       DAXWLH010000223         3013 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXWLH010000223 DAXWLH010000000
VERSION     DAXWLH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.229"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            85..606
                     /locus_tag="ACGE36_002621"
     CDS             85..606
                     /locus_tag="ACGE36_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFF8384967.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(1305..1870)
                     /locus_tag="ACGE36_002622"
                     /pseudo
     CDS             complement(1305..1870)
                     /locus_tag="ACGE36_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2049..2705)
                     /locus_tag="ACGE36_002623"
     CDS             complement(2049..2705)
                     /locus_tag="ACGE36_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384968.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
BASE COUNT          993 a          630 c          424 g          966 t
ORIGIN      
        1 catctaattt ttagacaaac agtctaaatc gttgcctttt tttgtcagac atgtataaac
       61 tataagcgaa agaggtgttt taacatggat atatcagtga ttgatgcaac gaaaacaaat
      121 acacaaaaag gaatccttta tggttcatcg aatgctccta aaaaaatggt cgaattcatc
      181 aacctatctt gtccgtattg ccgacaatgg tttgaagaat cttatgagct attagaagaa
      241 gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt tcgataaaga aaaagaaagc
      301 ttgcagcgag gaaacgtgat gcaccggtat ctcacaatca gcgatggtca aaaagcgatc
      361 aaagagatca aacaaatatt cgatactcaa gacgagtgga aacatctttc tctccaagaa
      421 gtagcggatt ttgcagtaga taaactaaag ctgacagaac aaaaagacga acagctgagc
      481 caagctgtca tcaacgaagc tgagcaagca catattcgat ttgttccgac tgtcatcttg
      541 ggtaaagaaa tctttgatga atctatttca atagaggaat taaaagaatt gatccaaaca
      601 aaataaaaaa ataaggagcg taggacaaaa gtaaagaaca cttttgtctc acgctctaaa
      661 cacctataaa cggcggaagc agaagcaact ccttcttatt tcttggagtt aaacgcttct
      721 gtcccagcct ccttattccg aataaatggt gtccaaaagc ttgcatctgc cagattatct
      781 catttcttat gacaagctga gcaaattatc tccaccaaat aatttattca tatttttgac
      841 ttgcgcatac taactaattc ttaaattttc atacaaaatt tctcaaattc ccttaacgag
      901 taatatttat gtacggtgat tcacactagt atcccaatca ctttttaagt tatcccgatt
      961 tcaattctac ttcaaaaata gttgctttta tgtatgctgt tagttgatta gatggtcgtt
     1021 tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt agcttatacc tatctcgtct
     1081 accaaacgca agatggcaaa cacaaatgca tacgcatctg tatttgccac tttgattcgt
     1141 ataaagctaa caactcctaa ctcctctcat ttaatagttg ctctcttttt tattcagata
     1201 catttttgct gatcacagcc tagtatttca atggttttga acactaaacc aaattgttcc
     1261 attacaacat tcactggctg atgaaatgga ttaatagatt tcgattattc aaagcgattt
     1321 tctattggtg gattgtgttc ccaactaaca agacggattc caaagtcatt atataactct
     1381 tcaagagtta accctttcct gttcccaata gatgtatctc tcccacgctc gatataaaat
     1441 atggagtttc gaatgccaag acctggcaaa tctttccttg ttacagttac actgctacta
     1501 acttccccat ataaaatcca gctcctccaa aatgataata tatttttcga ccatcatcta
     1561 actcatgtaa caataattgt tgtgccctct tgtcactcgc caaatgcaaa cgccacgtgc
     1621 gataccatgc catgctacct gcatctgcag aaaatccatc aaataatgca acaattgtat
     1681 cttctatcac aatcctttcc ccatctactt catattctaa tctaaaatca aactcccctc
     1741 gggtaatcgc aggtcttggt ggcttaggac ccaatattat tctacttaaa accgcattcg
     1801 ttcccagatg aaacacagct ataacaacaa taatgattaa tattttgttt ctatttgata
     1861 aatttctcat tattcgcaaa tcacctctac attctcacat actagttcgt aatactcctt
     1921 taatatcaaa atatctactg atcgtaataa tattcgctat gaattcctac tactcgtata
     1981 agaaaggcca aatcatcagc ttttacttgc tgatgatttg gcttgtttct atttatcgta
     2041 attgttttct aatcaccaaa attgttaaca atcggttcag atatttccca attcactagc
     2101 cttatattat attgatttaa caattcttct tcatttattg tattttgatc aagcggacca
     2161 aattgttcta ataattcttt gtcatgtaaa atatcccttc tagtaatgcc tctttccctt
     2221 tcaatgaaag aagcactagg aaaagtatga ttataaccat tcaacctatc tcccatgtaa
     2281 taattagcag gtcctggaaa ataaaatatt tgcttattat cactaacttc taacagcaac
     2341 actaaatctt ctccgctggc taaacgactt gtccatctac gatatctccc tatgccttcg
     2401 ttcattccaa atccatcaaa ttcaactatt aacgtgtctt ctatcacatg taattcttca
     2461 ttcaattcat actcaagtct aaaaggaaat tctccatatg taatctctgg caatggtggt
     2521 gctggagttc tccaaataaa tacaataact gtgaatagaa aaaatccaac gatcgatata
     2581 aatgttatga gacctgctcg aagtaaaacc cgatataata aaaatgctgt atttcgcttt
     2641 gctatatcta gaaaaataaa aagtactgca atcataatga tagtaaaagt tgtaaaaaaa
     2701 cgcatttttg ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttggtag
     2761 ttcaagttca ggtataacgt attccaacca tacatctcaa ttactattta tgaacttatg
     2821 attatcactg aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg
     2881 ttgttttcga ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga
     2941 atcttgacca gaagtaagaa gagcagttct caaggatgat cgagcagttc cttccagttt
     3001 tttaattaat cag
//
LOCUS       DAXWLH010000224         3009 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXWLH010000224 DAXWLH010000000
VERSION     DAXWLH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.230"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            487..573
                     /locus_tag="ACGE36_002624"
     CDS             487..573
                     /locus_tag="ACGE36_002624"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8384969.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(875..1099)
                     /locus_tag="ACGE36_002625"
     CDS             complement(875..1099)
                     /locus_tag="ACGE36_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384970.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1465..1749
                     /locus_tag="ACGE36_002626"
     CDS             1465..1749
                     /locus_tag="ACGE36_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384971.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1752..2168
                     /locus_tag="ACGE36_002627"
     CDS             1752..2168
                     /locus_tag="ACGE36_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384972.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2305..>3009)
                     /locus_tag="ACGE36_002628"
     CDS             complement(2305..>3009)
                     /locus_tag="ACGE36_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8384973.1"
                     /translation="ELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFS
                     FEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKK
                     FVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIE
                     EFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSS
                     LTSDLPKISLRNWLEE"
BASE COUNT          996 a          567 c          473 g          973 t
ORIGIN      
        1 tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag ggttctaaaa
       61 aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc cgccaaaaaa
      121 tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa tctagctccc
      181 tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc agcagtcgcc
      241 tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc ctgagagctc
      301 gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct aaagcgcctt
      361 acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag gcttattttt
      421 gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg gaaggaggtg
      481 tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag cgctttgttc
      541 agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac tcagcccaca
      601 aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg ttcacttatt
      661 tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca aaagtctaaa
      721 atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag agctcgtatt
      781 tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa gctaaaaaac
      841 tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca tgaaattcgc
      901 tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt tgcattaatt
      961 tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat ttttcgtctg
     1021 ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt tgaaaatatt
     1081 cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta gaaataaaat
     1141 cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa ataaattttt
     1201 gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc ccgaatttta
     1261 aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag gctgtgccta
     1321 tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta gcttatgtat
     1381 agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac ataaattata
     1441 cataattgca aaaggagatt ttttatggac attttagttc ggaatattga tccaaagttt
     1501 gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga aaaatggagt
     1561 cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact tacgacttac
     1621 aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac agaaaatact
     1681 caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct tatcggagga
     1741 atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac cctagtcatg
     1801 ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac tcggaagcca
     1861 ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc agcaatcagg
     1921 caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc gcagaatttg
     1981 ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa gtatatcaag
     2041 aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga ccaaagaatc
     2101 atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca aattttctaa
     2161 aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag ccccatacaa
     2221 tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac ttgcttaatg
     2281 taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag aaatctttgg
     2341 caaatctgac gttaggcttg aaaaagtaaa ttctaatttg gcgattttat tgcccttttt
     2401 tgcttttatt tttgtgactt taagatcatt gaagtatttt tttagttctc gtaatgcagg
     2461 atttaatact ttttgatcaa tgttacccat ttgatagtat tctgggatat ccattaagct
     2521 cctaaactct tcaatacgaa ctacataata accagtagaa cggtactgca taagcagtcg
     2581 gaatagagtt tttgcatacg tagaacgcaa cgacgtgaaa gcagataatt caaatctact
     2641 aaattcagct gttaaaccat taataatata ttctaggtcc ggattaacac tgacttcaac
     2701 aaactttttt gttttattga tttcaaaacc agtaaacaaa acaaagtagc gaatagaatc
     2761 ctcatcttca gtacggtaag taagattaag catatcttta tagagctttt ctaaatcacc
     2821 gacaaaggct tttgtagcag tcatcttata atcacttaac tcttttaatt cctcgaaaga
     2881 aaatctaact tttttcaaac cacgatcttt catacgagcg cataatgcaa aaaataaatc
     2941 catctgctta gcattgaaat ttttcagcgg tactaaattc aattcatttt gatatttgac
     3001 tgttaattc
//
LOCUS       DAXWLH010000225         2966 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXWLH010000225 DAXWLH010000000
VERSION     DAXWLH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.231"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(275..616)
                     /locus_tag="ACGE36_002629"
     CDS             complement(275..616)
                     /locus_tag="ACGE36_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFF8384974.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            702..1409
                     /locus_tag="ACGE36_002630"
     CDS             702..1409
                     /locus_tag="ACGE36_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8384975.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            1396..1719
                     /locus_tag="ACGE36_002631"
     CDS             1396..1719
                     /locus_tag="ACGE36_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8384976.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1808..2845)
                     /locus_tag="ACGE36_002632"
     CDS             complement(1808..2845)
                     /locus_tag="ACGE36_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8384977.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT          831 a          619 c          532 g          984 t
ORIGIN      
        1 cttattcaaa acgctactct ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt
       61 ctaaccaaaa gaaaaaaata gataaaaaac taaagaaacg gtaaagaaag acgatttcaa
      121 tgaaaaatag caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct
      181 tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat
      241 attagtcatt tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg
      301 ttgctggata gattttccgg cattgttgat agattttatg atacttttct acattttgta
      361 agagaggttc cgctgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt
      421 cgaaccaccc taaccctagt gcagcaatca ttgttgcgcc taatgcaggt ccttcctcca
      481 tacttaaaca atagaccgtt gcatcgaaaa tatcagcttg gatttgtagc catgcaagat
      541 ttttcgcgcc tcctcccacc gaaacaatac gtgtaaatct gcgacccgct agttctttac
      601 gtttcaatcc caccattgac aaatgctcat taaaaattta gaatattcac aagtttaacg
      661 acaattatga aaatttatgt cgtgctaaag gagttttgtt attggatgaa aagttagata
      721 taaaaactgc gatcaaagac acgttaccta ccgttttcgg ttatatcggt attggacttg
      781 catttggtat cgttgggaaa gctgccggat ttcatccact agtcgtcacg ttgatgtccc
      841 tgctggtcta tgctggttct gcccaattta tcacagtcag catgcttgct agtcacagcc
      901 cattgctttc catcgttttc tcgacctttc tagtcaattc ccgaatgatt ctgatgagta
      961 tgacgattgc tccttatttc aagaaaaatc ggctgcttca aaatctgctg attggcacat
     1021 tgctgacaga tgaaagtttc gcactgggaa tgaataaatt gaactatact ggacaaaagt
     1081 taaactttcg ttggatgaat acagctaatt ggatttctta tttgacgtgg gtgggttctt
     1141 ctcttgttgg cgcactttta gggaatttta ttactgatcc gaaaaagttt ggtttggatt
     1201 tcgccatcgt cgctatgttt atcgggttac tttatcttca agtcatttct gatagaaaca
     1261 cctctaaacg attacagttg atcctgattg gtctcacttt gattctggta tatgtcggac
     1321 tgatttttat cccaagcaac ttagtgattg tagttgtaac actgattggg tgcggattgg
     1381 gggtgtggat caaacatgcc ttcttttgag tttacattgc tgactatttt aggttgtact
     1441 ttagcaactt ggctttctag ggtcctacca tttgtcttac tgaaaaaatt cgatttaccg
     1501 caaccgttgc ttgagtattt aagttttgtc ccgatcgtca ttatgtctgc tttatggttc
     1561 agcagtctat ttacccaaaa tatcggacat ctgccgcaga tcaatctcga aaatgctttg
     1621 gcttcagttc ctacactttt agctgctatt ctttcaaaaa gcttattggt gatcgtcttg
     1681 gcaggaattc tttccctttc gttgattcgc ttattataaa taaaaaaaca agagcttatg
     1741 gcaaattact gtcatgcgct cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta
     1801 ttcttgctta tccgttgaac ggaccactaa caacgacttt tcctaacata tgtcctgttt
     1861 ctacgtcttt ggttgcttgt ttcaagttct tcgcattcat cccatctgaa tagaccttgc
     1921 tcaatgtact gcggataatc ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc
     1981 cttgcgtttc gatttcatag ttatagtctg ttttagcgaa catatactcc cagtcgaagc
     2041 tgatagattg gtttttccaa acacctatat ctaacggctc tttgatccct acgatcgttc
     2101 ccacatgtcc aaatggtcgg acaaggtgct tgatttgatc aaagtagcgg gtaatatcga
     2161 ataatacagc gacatactct acttgatcga ttcctaattc acgaaaactt ttacttaagt
     2221 cgtgatgata atcgattgga tagtctacac ctgttttttt cagccattca aaattcttcg
     2281 gacttgcagt cgcgtagact tctaggccag cccaatgcgc tagttgattc aaaatcgaac
     2341 cgacgccccc ggatccgttg atcactaaga tttttttccc gcgattggca ttttcttctg
     2401 gagttagacc aaatttttca aataacaatt cataagcggt caatgaggtc aacggcaagg
     2461 ctgcagcttc ttcatctgaa agattttttg gtgcaagagc gacaatacgt tcgtcaacta
     2521 gttggtattc ttgattgctt cctgctcttg ttgttgtacc tgcgtaaaaa acgcgatcgc
     2581 cgacagaaaa tttttgcact tcgtcaccta cagcaacaac tttcccgact ccatcaaaac
     2641 ctaagatacg taaagcattt cttatcccgt tatcttggcg aagtttcgta tcgacaggat
     2701 taacagatac tgcgttgact tttaccagaa gatcccgtga actgggtaca ggtatctgtt
     2761 ctttttcatc aataaagctt tttggatcat cgatcggcaa cccttcataa aaaccgacta
     2821 ccttcattaa ttccttttct gacatacgaa ttcctcctta gcttcatcaa gttggagcat
     2881 ccatacagtg ttcatttcaa tatatatagt atgctatttg ttccaccttt tttattcatc
     2941 ataattattc tcttacttaa agtaaa
//
LOCUS       DAXWLH010000226         2964 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXWLH010000226 DAXWLH010000000
VERSION     DAXWLH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.232"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..225
                     /locus_tag="ACGE36_002633"
     CDS             <1..225
                     /locus_tag="ACGE36_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384978.1"
                     /translation="ERTGKKFSRNDYFLAIIRQDSEIDLIDYKKSEFDLVVEKLLLSN
                     EQMVESISELTSVYERLFNFLISERSDLNK"
     gene            222..689
                     /locus_tag="ACGE36_002634"
     CDS             222..689
                     /locus_tag="ACGE36_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384979.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKANMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSIHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEKESSRLGDGTMFSSPSLRFTTD"
     gene            771..2291
                     /gene="mobP2"
                     /locus_tag="ACGE36_002635"
     CDS             771..2291
                     /gene="mobP2"
                     /locus_tag="ACGE36_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8384980.1"
                     /translation="MTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDYISRQE
                     AIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTRKKAIL
                     ENIDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFLVREGY
                     YNPETNELNENILYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAMERKNT
                     REIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRKNEFEKISKLRKEVPLELKQSVK
                     DSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDN
                     PKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEE
                     KNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREKNEVHF
                     KKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAHEFEIGMN"
     gene            2313..2642
                     /locus_tag="ACGE36_002636"
     CDS             2313..2642
                     /locus_tag="ACGE36_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384981.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            2644..2907
                     /locus_tag="ACGE36_002637"
     CDS             2644..2907
                     /locus_tag="ACGE36_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8384982.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSIVKEFLYSNPKIALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
BASE COUNT         1275 a          327 c          525 g          837 t
ORIGIN      
        1 gaacgtaccg gaaaaaagtt tagtaggaat gactattttt tagcaatcat tcgccaagat
       61 agtgagattg atttgattga ttataaaaag tcagaatttg atttagtagt tgaaaaacta
      121 ttattgtcaa atgagcaaat ggtagaaagt attagcgagt taacttcagt ttatgaacgc
      181 ttattcaatt ttttaattag cgaaaggagt gatctaaata aatgaactac ccttatttca
      241 aagtatcagc aacagaagat gtaaaagaaa aattcaataa gtgctttgaa gtaaacaagt
      301 cagtatttca aacaaaagct aatatgttcc gatatgttgt ttcaaattta ccaatgttaa
      361 caaaagccaa tcaaaatttc cgtgatccag aaaaagaaga gttagctaaa aacttagaag
      421 aagcaaaatt aaaaattagc gaacttgaaa cgataataga attgcaaaaa cagcaacttg
      481 aaaactttaa taaaaaagaa gacagtatac acaaagatta tagtgaacaa ttaaatagta
      541 tagaagagtc tgtctataaa ctattaatta atagtggatt agctagtaaa gaattagaag
      601 aagagcgtgc tgaaccagaa aaagaaagta gtcgtttagg agatggaaca atgtttagtt
      661 caccgtcatt aagatttaca acggattgaa cactaaaaaa ttctaatgca acttagcatt
      721 aagtacaaaa ccgcctgtag aaaaaaattt tgaaaatggt gggtgatgat atgactgaac
      781 atatctttaa cagaaaaagt gcgagcatta ttcttaaagc aaaatttgaa actggaaaaa
      841 acaaaaagaa gaatcacatg gtagaacttc aaaaatttgt agattacatt tccagacaag
      901 aggctattag gcaagataaa ttagatcatg attattccga agatgaacta aatgaattag
      961 ctagaataga aaaagctttg gaaaaaatag aacaggaaac agatcaacca gtgattaaag
     1021 atttgcgtga aatggataaa tatattgatt atatgacacg taaaaaagct attttagaaa
     1081 atatagataa tgaaatagta aacggagctt ttagtaatac aaaaagatat atcactaaaa
     1141 aagacatttc aaaaataaaa gaatcggtta tagaagcaaa aaataatgga tcggtgatgt
     1201 tccaagatgt aatttcattc gataatgatt ttttagtaag ggaaggatat tataatccag
     1261 aaacaaatga attaaatgaa aatattctat atgaagcaac aaaatctatg atgggaaaaa
     1321 tgcaagaaaa ggaagaacta gttgatcctt tttggtttgc aacgattcac agaaatactg
     1381 aacatatcca tattcatgta acagctatgg agcgtaaaaa tactcgtgaa ataatggaat
     1441 atgatggtgt tttacaagct aggggtaaga gaaaacagtc tacgttagac gatatgattt
     1501 ttaaattcgg tagcaaaata ttagatagaa agaacgagtt tgaaaaaatt tctaagctga
     1561 gaaaagaagt tcctttagaa cttaaacagt ctgtaaaaga ttctttgatt caattatatg
     1621 tagaaaataa ttcaagatat gacaaagaat tagtaaagta cctaaaggaa ttaaaaaaag
     1681 aaatacctag tacaacacgt ggctacaatg agcttcctga agaaacaaaa gaaaagattg
     1741 atgaagtcac aaattatatg acaaaggata atccaaaaag aaaagaatat gatcgtatga
     1801 ctaaagaaat tgatgagcta tatcaaacaa cctacggtaa acgctacaat ccacaagaat
     1861 attatttaaa tagaaaaaaa gattttaatg ctcgaatggg taatgctgta gtaggacaaa
     1921 tcaaactttt aaagcaatct gaagaaaaaa atttgaaaag taatgaactt aaaaatctat
     1981 tagacggaaa aaaattaaac gttaagtttg atagagaaaa accagaattt aaaacgaaat
     2041 atgctaaaca aagttatgat gatttccaaa aaagattaga agaacgacaa ttagcacgtg
     2101 aaaaaaatga agtccacttc aagaagattt ctgaaaaatt taaagataaa aagcttatta
     2161 gaaaaattga tagagtaatc aatgatgatt taaatatgta tcgtgcgaaa aatgattatg
     2221 aagtagcgca acgaatgata gaacaagaga aaatgagaca agcacatgaa tttgaaatag
     2281 gaatgaatta gtagaaacgg agaatagaaa ttatgaaaaa cttcgatata gtgtgcagta
     2341 atacaaaaaa tatatattta cgtgagctac ttaacagtga ttcggaaact atagaagatg
     2401 taaaaaaaat catagtgttg tttgaaaaag aaaacatgga gctagagaat tgggggcttt
     2461 ttgaaatacc gattagtggg aattactgtt tttataattg gaagacagaa gacgatgtag
     2521 cttttgcaaa ttactttttt gataaaaatt acttttcccc attatatata gataagcata
     2581 gtaatgaaca agttgcttct tcaataaaag aagcaataaa attggaaaga gtgaggaaat
     2641 gatatgatcg attttagaac ggttatggat aaactaagta atggagattt tcgcgtattg
     2701 attgaactat acttaaatgt aacgtctata gtcaaagagt ttttatattc taatcccaaa
     2761 atagctttag tattagttgc agtactgata gtttttgctg tgatctcttt tattaagaaa
     2821 atgattccgc tattaattat aggtgctgtg ttgatattag gtttgtattt ccctgaagtt
     2881 ttggatcaaa taaaaaatat attttaggct ctaaaagctt ccagtagaaa attttttaga
     2941 ttacttgtac taacaacata aggt
//
LOCUS       DAXWLH010000227         2875 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXWLH010000227 DAXWLH010000000
VERSION     DAXWLH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.233"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            35..724
                     /locus_tag="ACGE36_002638"
     CDS             35..724
                     /locus_tag="ACGE36_002638"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8384983.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            738..2240
                     /locus_tag="ACGE36_002639"
     CDS             738..2240
                     /locus_tag="ACGE36_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8384984.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2253..2729
                     /locus_tag="ACGE36_002640"
     CDS             2253..2729
                     /locus_tag="ACGE36_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8384985.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT          933 a          389 c          570 g          983 t
ORIGIN      
        1 ccaaaaataa taaaaaattg gagcgtttag gataatggat aaaaagaggt ttttagatct
       61 tatttcaaaa ggtttgattg tttcatgtca agcattacct ggtgaaccat tttacaaaga
      121 ggaagaaagt ttaatgcctt attttgcttt ggctgctcaa agggctggag cagttggtat
      181 acgtgcaaat tctgttcgag atattattga aattagaaag tgtgtatcat taccaatgat
      241 tggaattatt aaaagagatt atccaccaga atctccattc attactgcca ctatgagaga
      301 agtagatgaa ttagtagaaa ccggtgttga ggtaatcgct cttgattgca ctcttagaaa
      361 tcgacatgat ggactatcta taaattcttt tattactaaa ataaaagaaa aatatcctca
      421 tcagttattt atggcagata tttctacatt tgaagaaggc aaaaatgcat ataaagcagg
      481 aatagatttt gtaggaacta ctttaagtgg atatactgag gaaagtaaaa aacaagatgg
      541 gccagatata gatttgatta aaaaattggt agaagaaggg atttgtgtaa ttgctgaggg
      601 aaggattcat actccagaac aagcaaaaga gataaaaaaa ataggagttg caggtattgt
      661 tgtgggtggt gctattactc gaccacagga aattgcatca agatttgtta aagcattaag
      721 ttagggggag aaataaaatg tttaaaaaat tttcacaatt aggacgcgct ttcatgttac
      781 caattgctat attaccagta gccggattac tattgggact gggaggagcg ttaacaaatg
      841 agagtgctat aaatgcttat ccatttttag atcaaccgtg gttgcataca attttaagta
      901 ttatgagtta tgctggaaat gcagtttttg caaacctagc actaattttt gcaattggta
      961 ttgctgtagg acttgcaaat ggagataaag gaacagctgg tttagctggt ggagtagctt
     1021 atttagtata tactgcaact attagtgggt ttcttgaact tttttcagca aaagatgcaa
     1081 cactagatac aggtgtagtg ggatcaattg ttgttggtgg tactgttgca tttttacaca
     1141 atcgttatag aaaaattgaa ttaccacaat tcttaggttt ttttggagga tcaagattta
     1201 ttccgattgt ttctagttta gctgcaatca ttattggttc tttcttttat ttaatttggc
     1261 caccaattca aaatggttta gtagttgctg gtgaacaaat tgcccaaatg ggtagtttag
     1321 gaactttttt atatggtttt ttattgcgtt taacaggtgc tgttggacta caccacacca
     1381 tttacccttt gttctggtat acatctttag gtggtaccga aactgtagct ggatcaacaa
     1441 ttgctggagc ccaaaatatt ttctttgccc aactagctga tccaaaccac actggtctgt
     1501 ttacttatgg tactagattt tttgcaggaa gatttgcaac aatgatgttt gggttaccag
     1561 cagcatgtta tgcaatgtat cgtgcaattc ctaagaaaaa tcgtaaaaag aatggtgggc
     1621 tttattttag cggggccctt acatcatttt taactggaat tactgaacca gttgaatata
     1681 tgtttttatt tgttgctcct tggctttatg tgattcatgc attcttagat ggattatctt
     1741 tttattttgc tgatatctta aatattcgtg tagggaattc cttttcaggt ggtttgatcg
     1801 actttttatt attcggtgta cttcaaggga atgacaaaac taattggtta aaattaattc
     1861 cttttggtat tgcatgggca tttatatatt tctttgtttt tacattcttt atcaaaaaat
     1921 ttaaggtagc tattcctgga atggaagaag aatcagaagt tcattctgaa aaagtaataa
     1981 acaaaaatac atcattacat gaagagtctc ttatcattat tgaagctctt ggtggaaaag
     2041 aaaatattga gtctgttgaa gcttgtgcaa ctcgtttgcg tgttgctgtt acgaatgggg
     2101 agattattca aaaacaaaca ataaaagaca ctggtgcaac tgctgtcttc gaagtgaagg
     2161 gtggcatcca agctgttttt ggagggaaag ctgacttatt aagtcaagaa atcaatcaaa
     2221 tattaggaaa agataattag gaggttttta atatgtttga aattttcaaa aagaaaaaac
     2281 aagaaacttt ttatgcacct tgttccggaa aaatcatttc catcacagaa gtaaatgatc
     2341 ctgtcttttc acaaaaatta ctaggtgatg gttttgccgt catacctgat ggttcaaaaa
     2401 tttatgctcc attggctggt cgagtaacaa atatatttcc tactaaacat gctattaata
     2461 taaaaagcaa ctcaggcatt gattatttga ttcatattgg aattgataca gttgaattac
     2521 aaggagagcc tttttcgatt tgtgttcaag aaaatgatga ggttactcct gaaacctcgt
     2581 tggttgatgt ggattttgac aaaataataa aatctggtaa agatacttct gttatagttg
     2641 tttttccgga gaaaaatcag ttcaatgatt taataataga taatggtcga attgctcatg
     2701 ggaattcatg tggaaaaatc actttttaaa aaagtgtata taaaaagcta taaaataagc
     2761 taaataggat atttgtcaaa tgaagttggt gaacagaaag aactgttttc tttctgttca
     2821 tttttttatt ttattagtaa tgattcccca tgccaaatca tgatccgtaa tttaa
//
LOCUS       DAXWLH010000228         2837 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXWLH010000228 DAXWLH010000000
VERSION     DAXWLH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.234"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            63..725
                     /locus_tag="ACGE36_002641"
     CDS             63..725
                     /locus_tag="ACGE36_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8384986.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(801..2093)
                     /locus_tag="ACGE36_002642"
     CDS             complement(801..2093)
                     /locus_tag="ACGE36_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8384987.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            2263..>2837
                     /locus_tag="ACGE36_002643"
     CDS             2263..>2837
                     /locus_tag="ACGE36_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8384988.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALV"
BASE COUNT          881 a          581 c          536 g          839 t
ORIGIN      
        1 attgaaatca atcgatataa gcttgccttg catgcttgtt atcccttaaa aggaggaatt
       61 acatgtcttt aaaaaaatat agcttcctca gcattttttg ctatggtcta atttttatct
      121 cacccttgat cctagcttcg ataggacttg taaaatctac gtctgaattg atcacagcaa
      181 ctgcagtcat gtatatacta ggcgccgttg tcctagctat tttttatttt aagcaacgtg
      241 aacccttagc gatcgaatca gcggtaaaaa aagcttctgc tccaaagatt gttatttatg
      301 gattggttgg aatctttgtc gcattgattc tgcaaagcat cgctgtcatg ttagaatctg
      361 tattgtttgg caaagcaacc ccttccgaga atacacaaaa tatcatccag atgatcatgg
      421 aagcacccgc ttttattttg gcgacaactg ttgctggacc catcatggaa gagttcgtat
      481 tccgccgcag tatactgggg atcatcagtc gatattcaaa tttttgggtc ggagcagtga
      541 tcagctcttt gctgtttgcg tttgctcata atgatggtca tctgctaatc tactttttcc
      601 taggtttctt cttcagccta gagtacaaag caactggtcg gatctggaca tcgatgatca
      661 cacacgtagg aatgaacaca ttagttgttc ttgttcaatt agctgtccaa aaagggctag
      721 tataaacatg actataaata ctcctgtcat cctagattgg tgattttccc aatctaaagc
      781 gacaggagta tttttgtttt ttagtcgttc atcttttctg ctgctccatc attttttgtc
      841 tgtctttcct ttttgcagtt tgcagaatct atttttttca aaaatgggaa ataaatgaaa
      901 aaactaattg acaacacgat accttgtaaa atcgctgcag gcactccgcc tactaagaaa
      961 cccgagagga tcggcggtgt tgtccaaggg acttggactg ctgtaaataa aggaacgaat
     1021 ccaaaatata aagcggaata ggtaatcaat cctgaaacga ccggtgcgaa aataaaagga
     1081 acagccatca tcgggttcat cacgatcggt gtggaaaaaa taattgattc attgatgttg
     1141 aaaaaggctg gtaaaagagc aagatttcct aattctctgt atttcttcga tttcgcaaaa
     1201 accgtcataa aaaatacagc ccctaacgtc aatccagcac ccgtaaccgt catgaactga
     1261 tcgagaaatt gctgagtaac gatatgtccg ccatttgcca ccgtcaactc ttgtcctgag
     1321 gctaagattt ctgcattcgc taatgaattc gcttgcaaga cgggactaac caacccattg
     1381 accatcgcag atccatgaag accaaataac cataaaaacg gaacaaaaaa acctaataaa
     1441 agcacaccaa acaacgtgtc tatgaacgat tgaatgggaa gctggacata tgtccatact
     1501 ccttgaacaa cagtcattct gaacaactgt tgaaaaaccg ctgaaccgat aagcgtaaaa
     1561 gtgactataa caatgattgg aatcaatgaa gtaaaagact gccaaacatt ttcaggtatc
     1621 ttctctcttt gcttccaccc tttcgcaagt cttactaaac gtgcatagcc ccatccgatg
     1681 atgagcccag ttgtaactgc tcctagtatc ccttcagcac ctgtccagac agtatctatc
     1741 tgagtattta gtggctgaat aagcagtaga taagcagaca aggctgttat gccgactggt
     1801 aagcgatcga tgtcttcttc ttttgcataa gcgtatgcaa tggcaaaaac gatccaaaag
     1861 gctatcacat caaatgtagc ctggtatact tgtgtaaaat acatagaaaa tcctgttttg
     1921 tctatccact gagcgatgta atccacagga aaatgagcaa ataagaaaaa cagcgagcca
     1981 acaataatta ttgggatcgt gtaagtcatt ccattacgca aagcagttaa gggtctaaga
     2041 ttcacaacag atgttatcat tggtaaaaag gcacgatcta accactcttt catttcttat
     2101 ctccttcaac ttctattttt atcaatcagt tttcttaatt taactatacc caatgatcac
     2161 gaaccatgaa aggaatgcgc actgagcttt tcaaaaatcg gattctttga cgaatattca
     2221 gcaactgctt acaatggaca ggaataaaaa aggaggtttt ttatgaaaat tgcactcatt
     2281 ggagctactg gccatgcagg ttcatttatt ttagacgaag cattgtccag agatttagac
     2341 gtgacggcaa tcgtgcgtca tccagaaaaa ttgccatcag atgttccgtt tatacaaaag
     2401 gatttatttg agttgacaac tactgatctt gcatcttttg atgttgtcgt tgatgctttc
     2461 aacgctccca aaggaaaaga agaaatgcat caaacttctt tgtcccattt gattgagatg
     2521 cttgaaggaa ccgatacacg cttaattgtc gttgggggtg cctcttcgtt attcttagat
     2581 acagaaaaga cgactcgtat gattgaccaa gtacctaaaa atgcaccctt ctatccaaca
     2641 gctttcaata tgtacgaagg attgttaaaa ttgaaacaat ctaaaaatct acattggact
     2701 tatatcagtc cagcttcttt atttgatcca catggcaaac gtacaggtac ttatttgtta
     2761 agtgatgatt atttacaaag aaacgctgct ggcgacagta cgatcagcat ggcagattac
     2821 gcgattgcat tagtaga
//
LOCUS       DAXWLH010000229         2654 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXWLH010000229 DAXWLH010000000
VERSION     DAXWLH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.235"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(195..773)
                     /locus_tag="ACGE36_002644"
     CDS             complement(195..773)
                     /locus_tag="ACGE36_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8384989.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(793..1461)
                     /locus_tag="ACGE36_002645"
     CDS             complement(793..1461)
                     /locus_tag="ACGE36_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8384990.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1476..2546)
                     /locus_tag="ACGE36_002646"
     CDS             complement(1476..2546)
                     /locus_tag="ACGE36_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8384991.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT          782 a          573 c          397 g          902 t
ORIGIN      
        1 aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa
       61 atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata
      121 aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac tatttttctc
      181 cctatataac agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc
      241 gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact
      301 aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt
      361 gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga
      421 tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc
      481 agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg
      541 cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact
      601 gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg ggtgatccca
      661 actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca
      721 gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca
      781 cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc
      841 gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt
      901 tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt
      961 tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct
     1021 ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag
     1081 ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct tatctactaa
     1141 taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc
     1201 aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga attcttgatc
     1261 attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt
     1321 acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat
     1381 ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc
     1441 tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt caacggatcg
     1501 atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac cagcatcaac
     1561 aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca
     1621 agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat
     1681 gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca
     1741 ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc
     1801 gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca
     1861 ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg
     1921 attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg
     1981 aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg
     2041 gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta
     2101 tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg
     2161 ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg
     2221 aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt
     2281 gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc
     2341 atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac
     2401 ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat
     2461 accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc
     2521 ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca
     2581 acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat
     2641 ttcattatat gaat
//
LOCUS       DAXWLH010000230         2635 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXWLH010000230 DAXWLH010000000
VERSION     DAXWLH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.236"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            394..1773
                     /gene="brnQ"
                     /locus_tag="ACGE36_002647"
     CDS             394..1773
                     /gene="brnQ"
                     /locus_tag="ACGE36_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8384992.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(1927..2373)
                     /locus_tag="ACGE36_002648"
     CDS             complement(1927..2373)
                     /locus_tag="ACGE36_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFF8384993.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2400..2576)
                     /gene="rpsU"
                     /locus_tag="ACGE36_002649"
     CDS             complement(2400..2576)
                     /gene="rpsU"
                     /locus_tag="ACGE36_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFF8384994.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
BASE COUNT          706 a          503 c          496 g          930 t
ORIGIN      
        1 ataacgtcaa caaataaata tacagcaaag cgtcgaggaa aacagtaggc tattcagtga
       61 tttattccca gtgagtcatg taagtgagaa atgacaataa atcgaataga cgaacatcat
      121 ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc tccattatca ggctagcgga
      181 taactttatc gaaaagcgtc cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac
      241 cgttctagaa tctagagccc ttttcccaag tggaaaaggg ctttttttat attttaaggt
      301 ggtggttttc atggaatctg atattagtgg tatttctagt ttctgcaacg tttattttgt
      361 tactcaaaat gataattatt ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg
      421 aagcaattat tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc aggtaatctg
      481 atctttcctg tacacttagg tcaaatggct ggatcaaact ggatcagcgc tggtgtagga
      541 tttgccatct caggcgctct tttcccatta ttagctatct tagctgtagt agtgacaaaa
      601 agtgatggtc tgtatgactt agctaaacct gtcggaaaat ggtatgctgc cttgttcttg
      661 atccttgttc atttaacgat cggaccattc tttggtacac cgcggacagc agcaactgct
      721 tttgaaatgg ctgctaaacc ctttttgcct gaaaaatatg attcattagg tatgttttta
      781 ttctctgctc ttttcttttt acttgcttat ttccttactg tacatccaac acgtttggtc
      841 aaatatgtcg gaaagtattt aaactccttg tttcttgcat tattagctat catttttgtc
      901 gtttctttat tccaacccat ggggaatcta aaccaatctg ctgcagattc ttatcagacg
      961 aacgctttga caaatggcgt tttagaagga tacaacacgg ttgatgcagt agctttactt
     1021 gctttaagtg tcacttttgt ccatgcagtt cgtgatcttg gttttaaaga taaaaaagtc
     1081 acagaattaa cagcaaaagc tggtagcctg agcgttgcgt tagaagtcat gatttatttt
     1141 ggtctggtcc ttctcggtgc gatgagtttg aataagatgt ctttatcaga aaatggcgga
     1201 actgcacttt ctcagatcac taatttttat ttaggtaact tcggtgctag cttcttaggg
     1261 gtaatggtca cattaggtgt cttcactact gctatgggac tcgttgtttc ttttgcacaa
     1321 gatttccata aactgttccc taaagtcagt tatatgacat ggctgcggtt aacgactttt
     1381 gtctcttttg tcgttgcgaa tgccggattg gataatatca tccaatggtc tttaccagta
     1441 ttgatgcttt tgtatccatt gtcacttgcg ttaatcttac tttccttaac agcaaaattc
     1501 ttccaaaaga gtccatttgt ctatcaagta acgatgttct tttctgccgt tccagccgta
     1561 ttggatatgt tggcaaattc gcctgcatta gtgagtcagc aaactgtcgt agcaagctta
     1621 ctaggatttt atcatcatta tgttcctttt gctgcattag gtcttggctg ggtcgtccca
     1681 acgttgcttg gttacgctgg cagcttgctc ttttattatg tatatcgcgt atcaggatat
     1741 aaacaagaag caagtgagtt accagaagaa taaccaaatc aaacacaatt taacataaga
     1801 gatattaaac aggaaaaaaa cagctagaag aaagaataag attctttctt ctagctgttt
     1861 tttagttctt tttttacgaa cacaaaaaac tgcggataat cactgtccgc agtttaaatg
     1921 ataaaattaa gattgtaata gttctttcac aatcgcattc acttggtttc catctgcttt
     1981 tcctttgact ttaggcataa ttgcacccat aactttgcca aattctttca ttgatgaagc
     2041 accagtttga gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg aaagttgttc
     2101 cggcatatat ttttcgacta ttacaatttc actttttact ttttcagcta aatcgtcacg
     2161 tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt ttcatttcac gagacaacac
     2221 cgttagttct tcttcttctg tcaagtcgtg accttcttta atctgctcgt tttgtataga
     2281 tgacttgatc attcggatga cttgtaaaga ttccttatct ttcgctttca tcgctgcttt
     2341 catgtcatcg tttaatctac ttagtagtga cacactacca actccaattc atcacagaac
     2401 tagaattttt tgcgttttct agctgcttca gattttttct tgcgtcttac acttggtttt
     2461 tcgtagaatt cacgtttacg agattcttgt aaagtacccg cttttgacac ggaacgtttg
     2521 aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt tgacatgtta
     2581 attccctccc tccgagctta aaaagtaacc gtaattgtta ttgaaatata tctca
//
LOCUS       DAXWLH010000231         2586 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXWLH010000231 DAXWLH010000000
VERSION     DAXWLH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.237"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..213)
                     /locus_tag="ACGE36_002650"
     CDS             complement(<1..213)
                     /locus_tag="ACGE36_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8384995.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLEN"
     gene            complement(544..777)
                     /locus_tag="ACGE36_002651"
     CDS             complement(544..777)
                     /locus_tag="ACGE36_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8384996.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(792..1742)
                     /locus_tag="ACGE36_002652"
     CDS             complement(792..1742)
                     /locus_tag="ACGE36_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFF8384997.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1739..2581)
                     /locus_tag="ACGE36_002653"
     CDS             complement(1739..2581)
                     /locus_tag="ACGE36_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFF8384998.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
BASE COUNT          864 a          479 c          320 g          923 t
ORIGIN      
        1 attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt
       61 aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa
      121 agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt gctgaaatcc
      181 ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta taatataatc
      241 tctggtttct atgggtactt cctcctaata agatgaatta actttattat cttacacagg
      301 acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc taaactaatg
      361 agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa
      421 atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta
      481 ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta aaaataaagt
      541 cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
      601 aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac ttacagatgc
      661 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
      721 taattcctga attgctagta aataaattag tatagtagtc gttttaattt gtaccatacc
      781 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
      841 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
      901 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc cacactgctt
      961 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
     1021 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
     1081 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
     1141 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
     1201 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
     1261 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg atcttccttc
     1321 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
     1381 tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc atttcctcca
     1441 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
     1501 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
     1561 attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt aactactact
     1621 tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga acatgccccg
     1681 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
     1741 ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
     1801 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
     1861 caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata aatatcatag
     1921 ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac attgcaacaa
     1981 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaaccatca
     2041 atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat ggatcaaaag
     2101 aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa accgaaacgc
     2161 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aaaatatgaa
     2221 ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct aaaaaagaac
     2281 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
     2341 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
     2401 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
     2461 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
     2521 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
     2581 tattat
//
LOCUS       DAXWLH010000232         2572 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXWLH010000232 DAXWLH010000000
VERSION     DAXWLH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.238"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(69..971)
                     /locus_tag="ACGE36_002654"
     CDS             complement(69..971)
                     /locus_tag="ACGE36_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8384999.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(968..1660)
                     /locus_tag="ACGE36_002655"
     CDS             complement(968..1660)
                     /locus_tag="ACGE36_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFF8385000.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(1703..2344)
                     /locus_tag="ACGE36_002656"
     CDS             complement(1703..2344)
                     /locus_tag="ACGE36_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFF8385001.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(2382..>2572)
                     /locus_tag="ACGE36_002657"
     CDS             complement(2382..>2572)
                     /locus_tag="ACGE36_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385002.1"
                     /translation="NFYRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGA
                     VSKMVKSGNVANISYLMI"
BASE COUNT          711 a          574 c          423 g          864 t
ORIGIN      
        1 aaaattctgt cacaacccct cttttttgat caacggtgtt caataaacag cgttctcatc
       61 tgcttttctt agctctgctg gtccagtaac cgagtaagct ctttgataaa tctttgagcg
      121 atttcactga gcggacgttt cgccgaataa atgatataaa attcatttag ttcgccttct
      181 gccaaaggga ttaaccgcaa gtgctcgcga atccctccaa tcacaatacc agatcctgaa
      241 gcgtaagcat cagtttcaag aacgatattc atcaaagtac ctcgatcact cgtataaatc
      301 actgtttctt gatcagggat ctcgataagg tcttcataaa aatacgggaa attatttcca
      361 tcctgtgtaa aacgaacttg aggataagat aacaattctt ctttggtgat ctctgcgtgt
      421 tcagctaatg gatgtccttg ccttaaaaaa atatgcgttt tgaattttcc aatgctgcgg
      481 ccacttagct ctccttgttg taaatatctt ctgataccag cttgattatg ctcattcata
      541 taaataatgc cgatctcact gtgctgctct ttgacttctt cgatcactct cgctgttgtc
      601 gtctccatga tccgaaattg tttgatctcg tctttgaatt cctcgagtag ctgtgcggtc
      661 aaaggaccta agaaatcata atgctgggca gcgatcgaaa aattgtctct cttttcactc
      721 gttagatagt gagtttctaa tgtatcaagc tggaccaata ttttctcggc acgatttaag
      781 aagtcttgcc cttcttctgt taaatatgcc cctcgattcg ttcgagtaaa cagcgtgatc
      841 cctaactcgg tttctaattc tttgatacca gctgaaagac ttggctggga gacaaacatt
      901 ttctttgccg cttctctgaa actaccattg tttgctacag cgaccacgta tttcatctgc
      961 tgaatattca ttgctcaaac tcctcgaaat aataaatttc tcttccattc aagggctgga
     1021 ttggtcggtt gttcggtcga gcatactgac catcgaaggg gagaagaaac tcttggttca
     1081 gttttcctac atgatccatt acaaaccatt ggattggccc gctggtaggc ggagttgtca
     1141 atgagccaat ataatggaaa tggctttttt gctccggaaa gaacacatcc ggatttaccc
     1201 aatgctcatg tttttctaag ttccaacttt ccccattttc ttgattatac agccaacctt
     1261 cctgtaccat tagctggtac atgacaccca caactagatt ctcttttttg ctgttcatat
     1321 gaacaaaatg aaattcaata tcttcctgtt ttccgttgat gatatgttca gaaggcaagt
     1381 ggaaatgaat atctgtcaaa taatagcgtt ctttcttgaa ctctacataa ctgagttggt
     1441 caaaaggtac taaatgaatc gtgtttttga attccttgtc agtaaaccgt tgcttttgat
     1501 aatgaaagga aagcgtgtct ccttctattt tggtttcttc ccccttggtc aaatgaatag
     1561 gcgactgcaa tggaaattcc gcacctctcg caaaccaatc acataaactc gcccaatttt
     1621 ccggaccttt gtctccagaa tagccccatt cgacatccat attctttttc aactatactt
     1681 ccccctgcat ctcatatcat ttttattttt cttctttctt tacaatcgtt gagacggttt
     1741 ctaaaaacgc ctcttttcca taaaattcct ggttcaatag ttccggaaat atctctttaa
     1801 agaaatactg aacagaaggc cgatgttcaa agcttttaat cgtagtcact gcctcaatga
     1861 acatctcctt ataaactttt tccgggttat ttttgatgat ttcatcaaaa gattcataaa
     1921 gaaggtcaac cttatctgaa atggctaata gttctccttc tacggtttca tcttttcctt
     1981 cgaacaaccg acggcgataa acttcttgga actcagctgg aatctcttgg ttgatgaatt
     2041 cgtccgtcat cttttcttca actgtttgca gcatccctct taactctttg cttgcgtatt
     2101 tcaccggcgt tttgatatca ccaataaaac gctctgtgta gtcatgattc aatgattttt
     2161 catagagcag tttccaatcg atcggttgtc cattcgcttc ttcaatgtct cctagaactt
     2221 gcgcaataga tgctacacga tacgaatgtg ctgcaactgt atgctctgta tatttaaaaa
     2281 atccaggtgc ccttgtgatc gtctctaaat tgttcaatcc taaaataaat tcattcaacc
     2341 ccataaaagt tacctccctt tgagatttct ttttccgaat atcatatcat taaataactg
     2401 atattcgcaa catttccgct ctttaccatt tttgaaacag ctcccttacc aaaaaataga
     2461 gaaaatctat atataataga aaaaagagaa gagattattc tcttctttct gctaaccagt
     2521 cttagaaaaa tgaattttaa aaaaatcaaa aactgatcac gatagaagtt tc
//
LOCUS       DAXWLH010000233         2528 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXWLH010000233 DAXWLH010000000
VERSION     DAXWLH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.239"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            17..655
                     /locus_tag="ACGE36_002658"
     CDS             17..655
                     /locus_tag="ACGE36_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385003.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            683..1111
                     /locus_tag="ACGE36_002659"
     CDS             683..1111
                     /locus_tag="ACGE36_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385004.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            1568..2479
                     /locus_tag="ACGE36_002660"
     CDS             1568..2479
                     /locus_tag="ACGE36_002660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8385005.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          970 a          361 c          511 g          686 t
ORIGIN      
        1 gaatggaggt taacaattgg agccgttgat agtgataaaa ggtgcgaaaa aaatcattcg
       61 aaacaaaacg attcttcatt tggatcgttt cactgcggat gctggggatc ttgtgactgt
      121 tgttggagaa agtggagcag gtaaaagtac gttattgaat atattaggat tgaatgatca
      181 gttcagttca ggacaattct atttgttcga tcaagagacg aaacaattaa ctgggagaga
      241 gaagcgcttg ataaaaagaa ataccatatc ttttcttttt caagattttg ggttgattga
      301 atatgaaaca atcgatttta atttagatat tggattgaag ttttcgaatt taaatgtttc
      361 agataagcga acagcaaagt atgaggcatt gaaaaaagta aatataaaca agaagctaag
      421 tacgcctatc tacacattat ctggtggtga gaagcaaaga atcgcctgtg cgagagtctt
      481 attgaagaat agcaaaatca ttttagccga tgaaccgact gggtcattgg ataaaagaaa
      541 taaggataaa gtagttgaaa tcttatcgca acaagcttcc caaggtacgt tagttatcgt
      601 agtcacgcat gatccatatc tgaccaaaat aggaaacaag aagatcatgc tttaaaaatc
      661 aaaaaggaaa ggatgtcgtt ttatgaaaaa agcagttatt atttgtgcac tagggatagg
      721 ttttgttgga ggaaacgcta tgtcatctga agtggaagca aattcagttc acggtgaaat
      781 ctattacaaa tctttaaagc caggagagat tcattttgat gctaaaggta atattattaa
      841 gtcaaattta ccagtagacc gggaaatttc tagaacagca attaatcata gctctggaag
      901 atgggtttat tatagtgata caacttgtac gagaaaaaca ggtcattcta accactatag
      961 tcgtgtctat aatcgtcacg gtgcaaaagc gaaagttgga aactctattc gatcagcaaa
     1021 tgcaacaaaa ggagtatggg cctatgcaag tgcgacagga aatagaactg acacattctc
     1081 atgttggtac aatccaaccg gttggtatta agtatccaaa agtttatata aaatagaaaa
     1141 aacatataat gaaacagcgt taaaagttat ttttggtagt tttaatatat tcatttttag
     1201 ttgtataaaa atatatcata ataaaaacaa caaaaaaatg tctggaaaat cgaacataaa
     1261 agtgtctgat ttgaatatcc cagcagcgga agaagaaaaa caaaatcgat aacatttgtt
     1321 ctaagaatta gttacgttaa atacggaacg caagatagac ttgaaaaacg gtaacaaaag
     1381 aagtagtata ttaaggtgtg atgtgctact tgacatttat tctgctgagc gaactttctg
     1441 gattgtgtag ccttatatct attcaggacg tatcgagcag attagtaaag gtgttcttga
     1501 tgattttcat cttgaacacc tttgttttta gcaattcgca aaaaacgaaa agtaggagaa
     1561 ttgataaatg aaaaaaagat ttttagcact tgccatcgta ctaggcactg gcttattgtc
     1621 aggctgtacg aatgccggtg aaaaaacagc cgttagttat aaaggcggta caatcagcga
     1681 acaagaagta atggatagct tgaaaaaaat gcaaggagca gattccgctg tgcaacagtt
     1741 gatcgtttat caagtatttg aagataaata tggagatgac gtatcaacca aagaaatcga
     1801 ttctcaatat gatcaaacaa agaaacaatt gggtgattca tttgattcac aattgaaatc
     1861 tgcaggttac acagaacaaa cattcaaaga cagtatcaaa caaagtctag cctttcaaga
     1921 aggtctaaag aaacatatca aattgaccga tgaagattta aaaacggctt gggaatcttt
     1981 ccatccagaa gtggaagctc aaattatcca agtagcaagt gaagatgatg caaaagatgt
     2041 gaagaaagct gctgataagg gagacgattt ctctaaatta gccaaagaca agtcaactga
     2101 tactacgaca aaagaagatg gcggaaaagt gaaatttgat tccactacta caacggttcc
     2161 ggcagaagtc aaagaagcag cctttaagtt gaaagatggt caagtttctg atgtgatcac
     2221 ttctaccaat gcttcaactt atactacgga atattatgtg gttaaaatgg tgaaaaacca
     2281 aaacaaaggc aacgacatgg ataaatacaa aaaagaattg aaagaaattg caacagacac
     2341 aaaattaagt gacagtacct tccagaataa agtaattggc gaagtattga aagacgctaa
     2401 tgtaaaaatc aaagacaaag actttgaaaa tgttttgagt acattcactt cagattctag
     2461 tacagcaagt agtaaataag cggtataaat aaaaaatatg aactatttaa atcaataaaa
     2521 ctgttcat
//
LOCUS       DAXWLH010000234         2523 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXWLH010000234 DAXWLH010000000
VERSION     DAXWLH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.240"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            155..619
                     /locus_tag="ACGE36_002661"
     CDS             155..619
                     /locus_tag="ACGE36_002661"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8385006.1"
                     /translation="MTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIV
                     MCVPLGFLLPIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVI
                     GFILWAVLSRVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            692..1621
                     /locus_tag="ACGE36_002662"
     CDS             692..1621
                     /locus_tag="ACGE36_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFF8385007.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1758..2516
                     /locus_tag="ACGE36_002663"
                     /pseudo
     CDS             1758..2516
                     /locus_tag="ACGE36_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
BASE COUNT          878 a          358 c          470 g          817 t
ORIGIN      
        1 aaaataaatg tattttatac tacctacact ttatagcctt ttatgtgtaa tggcaccatg
       61 tattatttat atggtatttt tttgcaaaaa aaataccctg aaacacaata tcctgatttt
      121 gatttttatg ctgtactgtt ggaaagtttt tgaattgact ggtacaggtg gactaacaga
      181 tattttttat agacctgata caggtaggaa attttctcta gtacagggca gtgttaatct
      241 tgtaccattc agtggactaa acattagctt tatattaaat atagtaatgt gtgtgccctt
      301 aggattctta ctaccaatca tatggaaaag ttataggaaa atagttcctg ttttcttggc
      361 tggggctcta ttttcactaa ttattgaagt ctcacaaatt ataactacaa gagcaacaga
      421 tattgatgat ttaatagcaa atacctttgg tgcggttata ggctttatac tgtgggctgt
      481 tttaagtaga gtggtgtcct tgaaggacaa gtcatctaca gtagattatt tagaacctta
      541 tatttatata ttgattggtt ttaccggaaa attcttactg tattttcctt tctggtttgc
      601 ttttgaagta ttatcctgat aattccatag aaaataatga gtttgagttg agaaagaata
      661 ttcttgtaca caactctgga aaggaggaag tatgaaaaga ttcttaaaat gggggcttgt
      721 tcttttagcg tctttgattg tgattctaat gtgtacagcg gttgttattt cttatgtgaa
      781 tcggccgtat gcaagagctg tttatgatag gattaaacgc attcttgaca aagattttta
      841 tgaccatcta tatctaggtt cttcatttga aataataaac cttgatacaa tggatattca
      901 tagctatatg aaaaaagacc aagaagaatt acccgcctca ttgagcaaat tatttacgat
      961 tgattatgcg cttagtgtcc taagtttaga tgaacagatt acaattagtc ctactgcact
     1021 tgacatgcag aaagctgact catctactgc acatcttcaa gaaggagata ttttatctgt
     1081 ttatgacatt atctgtgcta tggtcttacc ctcaggaaat gatgcagctt atagtttggc
     1141 aatttataca ggtggaaaac ttgatcctga tgcaaagaat gatgaagaac gctatcaaag
     1201 cttcatgaaa aacttagatc gttatctaag tgagcaaggc tataaacata ctcattttac
     1261 tgacgctgct ggctatgact ccaatgctac aacatcagcg gaagatttga caaaagtagc
     1321 tgcaaagcta ctaaagaatg ataaatttgc agaaatagtt gccaaggaaa cttatagagc
     1381 aaaatcaatt aagggaaatg tttataactg gaatactacg aatcaattta tgcaacaaga
     1441 taatagatac tttactgaag gtgtaaaagg tgtaaaaact ggatcgtttg atgggagata
     1501 caatctatgt gtgtattata catctagttc tgataaccac tatttacttg taagttttgg
     1561 ttcagagagt gatagtaggc gttatgtaga tgttcaaaat ctattgcata atctgccttg
     1621 aaatttcgtg cctttaagcg cgtatctaaa gtttctttga aaagtttgag aatgaagaaa
     1681 gtgagcagtc gaaaaacggt tgctcacttt tttgccctaa aatcgaaaaa atataaaaaa
     1741 gtcctttaca acacgtctca ggaaaaactg ctaagtcaat tctaatatct tgtggagcaa
     1801 gacttgcacc ttttgatata agagagctta gagaactaat gagcgaagat gaactagaac
     1861 tcgataaaat tggagataga aaaactgcct tgtttgtaat aatatcagat acagatgata
     1921 catttaattt tgtagtttca ataatgtatt ctcaattatt taatctacta tgtgataagg
     1981 cagatgatgt gtatggtgga agacttccag ttcatgttag gtgtctactt gatgagtttg
     2041 caaatatcgg tttgattcct aaatttgaaa aactaatagc gacaattcgt tcaagagaaa
     2101 tatcggcaag tataatactg caagcacaat ctcaattaaa agcaatttat aaagaccatg
     2161 cagatacaat agttggtaac tgtgactcta cactttttct aggaggaaaa gaaaaaacaa
     2221 cagtaaaaga attatcagaa accttaggaa aagaaaccat agacctatat aacacatcag
     2281 aaacaagatc aaatcaaaaa tcttttggtc taaattatca aaagactggc aaggaactta
     2341 tgagtcaaga tgaaataact gtaatggatg gtggtaagtg tatttaccag ctaagaggag
     2401 caagaccttt tctatctgat aaatttgata ttacaaagca taagaattac aaattgttgg
     2461 aagattatga taagaaaaac ttgtttgatg tagaagagta tttaaaacaa agatagcgta
     2521 aaa
//
LOCUS       DAXWLH010000235         2423 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXWLH010000235 DAXWLH010000000
VERSION     DAXWLH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.241"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(19..1785)
                     /locus_tag="ACGE36_002664"
     CDS             complement(19..1785)
                     /locus_tag="ACGE36_002664"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385008.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQGGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAACL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1745..>2423)
                     /locus_tag="ACGE36_002665"
     CDS             complement(1745..>2423)
                     /locus_tag="ACGE36_002665"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8385009.1"
                     /translation="QKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYS
                     KDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPERE
                     HTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMK
                     EKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWR
                     LDNEKFL"
BASE COUNT          763 a          470 c          347 g          843 t
ORIGIN      
        1 aatttaaagc ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga
       61 tagtttagtt ttctttatga gatttttgta tatttctgac cttcgataca actcactatg
      121 agtaccttga tcaactactt ttccttgatc catcactaca attttattta ccttttcaat
      181 agacttcaag cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact
      241 ttcctgaata cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa
      301 aattggcgca tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga
      361 aagtgaactt ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa
      421 gtcttcgcag ttagctaaac aagctgcttt tttcacttcc tcatctgacg cttgactatt
      481 tccaatacgt atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat
      541 agcgattttg ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa
      601 aatacagcct tcatcataat catacagtct agacactaat ttcaagatac tagttttccc
      661 ggaaccacta gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg
      721 atctaaaatt ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa
      781 agatttcaga ggactatctg atcccccttg gatactggtt tccttcatag ctctaattcg
      841 ctgtatttta ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga
      901 atcctttatt tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc
      961 cgttaacagt aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat
     1021 agaggataag gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact
     1081 ctcttccatt tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat
     1141 ctcttgctgc aattctatag tttcttgaaa ttcttccgag ttttctctca aagtatcgta
     1201 atatttagta ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa
     1261 taacgttggc aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga
     1321 aataaaaggg aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg
     1381 ctcaatacct tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga
     1441 aaaataggat aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact
     1501 ttctttataa gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa
     1561 aagcaaagta acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac
     1621 taattcgtct atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa
     1681 aaaactagaa atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt
     1741 gtttttataa aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga
     1801 ccaattgttt atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta
     1861 ttttcccttc tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag
     1921 ctatcataat aactgttttt tctttcatca gatgttgaaa agccttttgt agttcatact
     1981 cattatctgg atcaatagat gcactggctt catccataat aacaatcttt gcatctttta
     2041 aaattgctct ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac
     2101 taccaattaa ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta
     2161 atcttagcgc gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat
     2221 cgtaaatact ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa
     2281 ttgtgtcttt tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca
     2341 cattgtagaa acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta
     2401 ctaatgcata aattcttttt tgt
//
LOCUS       DAXWLH010000236         2406 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXWLH010000236 DAXWLH010000000
VERSION     DAXWLH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.242"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..554)
                     /locus_tag="ACGE36_002666"
     CDS             complement(<1..554)
                     /locus_tag="ACGE36_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8385010.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCI"
     gene            complement(554..1510)
                     /locus_tag="ACGE36_002667"
     CDS             complement(554..1510)
                     /locus_tag="ACGE36_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFF8385011.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1510..2166)
                     /locus_tag="ACGE36_002668"
     CDS             complement(1510..2166)
                     /locus_tag="ACGE36_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8385012.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          734 a          503 c          451 g          718 t
ORIGIN      
        1 tcaatacaat ataaaatagg cggctggttc ttttggttga taaaccgata attcagcaca
       61 ttatatgttt cctgtggcag tttggaacaa aaagaatcaa ccatatccag ttctttttct
      121 ccaccttcat gaccatagta gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc
      181 aatatttctt ccatagctgt gcgtgtagtc tctggcatag tgatgattgc tttatcactt
      241 tttgggagat accctagatt gaaaatccct gctttgagat tttgttgttc agctatcact
      301 tctcctaacg tttcgtggcc ttgtagaaac aatgagacac gttcttctaa ctgacgctgt
      361 tctagccttt cacgagtagc atcgattgct tgttgctgga tatcaaaact atagacatga
      421 ccagtttttc cgatgtgttc tgctagaaaa accgtatcgt ggccattgcc catcgttgca
      481 tctacgacgt gatcccctgg ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa
      541 gcagtttgca acatcagacg ctcacctttt tccgtacatc atattttcct tggaagctat
      601 ctctgcgaag aagttcttca tcaattgcat tcaagacttc ccattttttc aagctccaca
      661 ttggtccaat caaggaatcc cacggcgcat cccctgtcaa acgatggatg atgatctctt
      721 ttggtatcat ttccagctgg tcgcaaatga cattgacata gtcaggacgg ctcattaatt
      781 gaaggcgacc ttcatgatag tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat
      841 ggagcttgat tccttggata tctgaatcta aaatggtgcg tctgacattt tctttcatca
      901 tttctaaact ttcgcctggt aatccgttga tcaaatgcgt acaaacacgg atattatgtt
      961 tgcgtaattt agccacgcca tctaaataag tttgataatc atgtgctcgg ttgatcagtt
     1021 ttgaagtttc ttcaaaagtt gtttgcaatc caagttctac ccataaatac atccgctcgt
     1081 tcagttcagc aaggtaatca actacttcat ctggcagaca atccggtcgt gtaccgactg
     1141 ataatcctac gacacctggt aaattgacta cttgctcaaa tcgttctttg attatttcaa
     1201 ctggcgcatg ggtgtttgta aaattttgga aataaacgat gtattgttgg acatgaggcc
     1261 atttttgatg catcatgtcg atttctttct ggaattggat tggaagaggg tcttcaggtg
     1321 caacgatcat gtctcccgaa ccagatacac tgcaaaaagt acaaccgcca tgggcaacag
     1381 tcccatctcg attcggacag tcaaatcctc catcgatcgg caccttgaaa attttcccgc
     1441 caaactcatt tcgtaaagcg taattccaag aatgatatct tttatttcct tctgaataag
     1501 gaaaagtcat taaaagtcct tctttctgta agagttatct atttgttttc ttttttctct
     1561 atacactgga taagtcaggc acagccaaga aagactgagt aagtagccgc cgatgatatc
     1621 agtaggaaaa tgaacgccca gataaatgcg gcttatcccg acacttacta taataaacat
     1681 catcagcaat tgaagcaatg tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa
     1741 tagtaaagaa ccgtaaagaa tcatactgcc tgctgcatgt ccactaggga aactgtaact
     1801 tgtttcaact actaagtgcg ccaatgaagg acgctctcgc atgaaaaata gtttgatcac
     1861 cgggttgatg attccagcaa ttacaataaa tccagaaacg aaccaaaatg tttctgccct
     1921 ttttttctta taccacatcc ataaagccgc acaagcaaat aaaatgatga cggtaagagg
     1981 attaccgaat ttagtcataa agagttggta gctgttcaga tcaggataag aagctctgac
     2041 aatgcctgta aaccactgat cgaaaccaga aagccacgac agatggaact ttacgatcca
     2101 gcccaaaatg aaaaacagga caagaaagaa gctaccagcc acctgaaatg atgttttata
     2161 tttcatacaa ttcctccttt gaagcaatga cttattatag catttttggt ccagcgaaaa
     2221 aaacggtaaa gagataaaac caatgtatga ttcctctttg aataaccaat attcttataa
     2281 aaaaaataac tgtaatcgga aagacatttt ttggtaatgc aaatgaaatg ttagggaaat
     2341 aaaatcagtg tttttcgaaa tccttttaaa caagaacgta gacgtaggaa gaacttgaaa
     2401 atgaac
//
LOCUS       DAXWLH010000237         2403 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXWLH010000237 DAXWLH010000000
VERSION     DAXWLH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.243"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            26..238
                     /locus_tag="ACGE36_002669"
     CDS             26..238
                     /locus_tag="ACGE36_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385013.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(516..2315)
                     /locus_tag="ACGE36_002670"
     CDS             complement(516..2315)
                     /locus_tag="ACGE36_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFF8385014.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          840 a          440 c          450 g          673 t
ORIGIN      
        1 aaaaataaag aaaagaggga atgaaatggt aaaagcagaa gtaggaagat cttatatatg
       61 caaaccgatc ggagtatctc atgaactaat cggctgtatt gaacgtacat atatcaacac
      121 ggctgtcgtg tatgttgaaa gttatgacca acgagactat caacttatca atgaaagcaa
      181 gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct actgaattgg tttcttaaac
      241 agtatttttg agaattcgca agaactccct tataatataa ataaagacag aagggagcga
      301 gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa ttattcttgc agtgatctct
      361 gcaattcctg ctattttagc tattgttcgt gcgatgaagg aatcaagaga aagttaagca
      421 cacattatga gcgtcaaaca aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt
      481 gttgtataaa gacttttttc attaacgatt tttgattagc cgactcccaa aacaaaattt
      541 agcagtttat ctgaataagt gatttcttca tctgtttgtt tcacactttc atttagtagt
      601 cccatctcat cggttatgat accatccaca ccataaaaca tcatgcgact catggcatct
      661 gaatcgttga ttgtccatac atatacttct tttccgtctt ggtgggcaga attaatgaag
      721 ttacggttga tcgtactata ttccattgtc agaaaatcag caggcgtcac agggggccca
      781 acaatattga aaggtaagat gtagcccaca taaaagtttg gttctgcttc ttttaaatct
      841 gaaacggttt ggtacgtcaa actttgcaaa atatgatggt tctctaagat aactttgcgg
      901 tatttatgga caaatcgttc gacaaggtca tccgaatctt ttttagaggt cttgatctca
      961 ataagtaatt tttggtttag cttatgtgct ttttccaaat aagaatcaaa agaacaaaca
     1021 ggggcttgca ctccgttttc agtaactgta agatttgtca attctttcaa tgtaagatcc
     1081 tgtggtgttc catttacccc tgttaagtga cgaagatttg catcatgcat cactacgaaa
     1141 tgaccatctt ttgtttcctg aacatccatc tctacataat caggatgata gtctctgctt
     1201 gttttttcaa gtgcttctaa agtattttgc gccccatttt gggtactcac acctctgtga
     1261 gagacagtca ctggttttgt ctgaacagct gaagtcagat aatccatatt gtaaagacag
     1321 acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa
     1381 cgcaagttgg gggcttgtgg gataaaccac ttagggatct caggcaagaa gccttcgtca
     1441 tccatgaaat caacaatgat ataaaaaatc cctacggtag acatcacgat attgaataat
     1501 aaaatccctt gtaaaagcgt catagcaaac acagcgctga tcaatgcata gtctgggaaa
     1561 aattgttcaa ccatactttg tgccaaaata acaacgatat atcctaaaga agaaaggagt
     1621 aaaatcgtac cgccaatcac aataaattgg cccgtgatgg ccaaaagacg cgatttggtc
     1681 aatgaccagc tttcccgaat ggctgctcga aaagaacgat ctcgtagaat catttccggt
     1741 aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa tataaacgag taaaaaggaa
     1801 ctgacgatga tccaacgatt agcaaaaata aaatccataa tgaaagcggg gatttttatt
     1861 tttgacagta gatcagaatt aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa
     1921 gcaaggaaaa acaaaaaagt aatgggtctc actttcttca gctgaagtat cgtcaagtgt
     1981 aagagttgct tcaaagagat tggttctttc tttttaataa aaaaaacact catcaaaaga
     2041 aaagtaaatt cgaaataaac taacagtaaa atcgtcatca aaactacgat taacgagagt
     2101 agaaccccag gatgttgaga aaaaattgaa ggaatattgt cataagacaa atagtcaatt
     2161 gcccctcggt gtaaaataaa acgggtcgta ctgctcaata agggtaaaca aataaataaa
     2221 ataaagccat gcatcagcaa gacatcacgg aaataagctt gtgttccttt taaaaagtcc
     2281 caagcattac ctaagctacg tttcaaataa ttcatcctat cacctgctct tcacattgta
     2341 gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt gcagaccctt tcccagattt
     2401 aac
//
LOCUS       DAXWLH010000238         2390 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXWLH010000238 DAXWLH010000000
VERSION     DAXWLH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.244"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            197..2059
                     /locus_tag="ACGE36_002671"
     CDS             197..2059
                     /locus_tag="ACGE36_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8385015.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACGE36_002672"
     CDS             2059..>2390
                     /locus_tag="ACGE36_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8385016.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAXWLH010000239         2379 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXWLH010000239 DAXWLH010000000
VERSION     DAXWLH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.245"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..209
                     /locus_tag="ACGE36_002673"
     CDS             <1..209
                     /locus_tag="ACGE36_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385017.1"
                     /translation="AATILCCGLIFSQLSIYPRYQRLFIHFLNIQCASMPLEVIKKLL
                     INPIFMVSLVLLTGLVTWVLGKKE"
     gene            252..2351
                     /locus_tag="ACGE36_002674"
     CDS             252..2351
                     /locus_tag="ACGE36_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFF8385018.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
BASE COUNT          867 a          366 c          465 g          681 t
ORIGIN      
        1 tagccgcaac aatattgtgt tgcggtttaa ttttttctca attatcaatt tatccaagat
       61 atcaacgatt gttcattcat tttctaaaca ttcaatgtgc ttccatgcca ttagaggtga
      121 ttaaaaagct cttgatcaat ccaattttta tggttagttt ggttcttctt acaggattgg
      181 taacgtgggt attaggaaaa aaagaatgaa aagcttacga tcgtttaaaa aaatattaga
      241 tggagggcat tatggaaaat aaaattcatt gcactacggg aagagtaatt actttagtgt
      301 tgtttttgtt ccttcttctt tttgcgaggg gaactgcagt ccaggcctta gaaaaagttg
      361 atccgagtca aatcaaagaa aataataatc tggtgcttta tcatgatagt gattcattga
      421 gaaatatcga agtaactggg atcaatggat ttaagatact agatgagtat tcaactaaaa
      481 aacaaaaagt acttgcttgg ccaaaagtca aagatttgaa aagtacgtca aaaatcgttg
      541 cccattatca aaaagtaggt acttatctag gaaaagaaat tgattgtgac gtgacttttt
      601 ctaattttgt agcgcacgct ggggttaacc aagacggatt agctaaaaca attaatggca
      661 aaaagtatta taattatttg ctctttgata aaaatattta tcgaggattt ttcatgcagt
      721 caatcaatgt catgaatgca aaactagaat ttaagtatgc aggaacaaaa acaaaagtag
      781 ctttgagtga accacagcca gaaattcaat caaaaatgag atcgatgaga gcagctggta
      841 ttgatttaaa cgatacattt ctatccattt acagtttaaa tggacagtat cagcaacgtg
      901 tgtcttggta taatgacaat aatgaatctg tcggtgaacg taatattgat atgagagaat
      961 ttgttgggta tgaaaaaatg ggtagcttac cttattttgt cacaacagat acagcatgtg
     1021 cagaatacaa agctcctgcg ttatcaacaa acaatttaac ttcaaaagta gtgggaggac
     1081 gtgcagaaaa ggcttatagc tcgaatgatc atttcaccga tgttgtagga gctgatactt
     1141 atcacagaag tggtgtaacg tatacgcttc aaggcgcttc cccaacattc atgattggcg
     1201 caaatacgaa tagtatgatg tttagctttg atactgcatt gctatggaca ccacaaccat
     1261 cgaagcctac aaaagaagtg tttaacaaag ctaatactga agaggcagca cacaatattg
     1321 acaaaaaagt gattccacaa ggatcagatg tttactatca tattcaccaa aagtttgatg
     1381 cattaacagt caacacaatg aacaaataca aatcatttaa aatcactgat acctttgaca
     1441 gcaaaaattt tgatatggta tcggatggga aaaactatga tggcgcattg tggtatgttg
     1501 aagcgaatgg tacttggaaa aaagctgata caggaggaaa gttaactgta tcaggtaata
     1561 cagtcactta tactgcttct gacagctttt tagctaacgc tgcaaattac ggtagggatt
     1621 atattttgca gctccatatg agagcaaaaa atgatgcaaa aggaattgca ccaaatgtgg
     1681 ctactgtaac gtttaacaat gatccgaaca aaggaacaca aaaaacaaat acagtagaaa
     1741 actatttctc tcttaaacca aataaaaaag tggaacaaga tgggaaagat gttgatggtc
     1801 gaaatgatgg taaaaaagga acgcctacag ctcctttaaa tgcaggatct gaagtacaat
     1861 atctagtcac acaaaaatgg catacaaaag gtgtagacat tgcctcagat cattacaagc
     1921 aatttagtat tcaagatcca gtggaagatc gtttgacgta taaagaaggt tctgcgcgag
     1981 tgatagatat gagtacagga aaagatatta cttcagaagg tacactaacc tatgatagca
     2041 attcaagaac gctgaagtgg gaagcttccg ctgatttctt aagtaaaaat cttttagatg
     2101 gacgagaaat tcagctgata tttacagcta aaactccttt gaatgaagaa ttggatatcg
     2161 acaaccaagc aaaagtttca gtagaaagta ttgagaaagc aactaatgta gtaacgattg
     2221 gggtaaaacc taacttacct cagatcattg tgccaaaaac tggatcgaat catttagtgc
     2281 taactgttat tggaagtata ttatttctta ctttagcatc attgggctat gtagttttaa
     2341 aactcaaata aatataaaaa caaggctgag aatcagcct
//
LOCUS       DAXWLH010000240         2368 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXWLH010000240 DAXWLH010000000
VERSION     DAXWLH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.246"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..278
                     /locus_tag="ACGE36_002675"
     CDS             <1..278
                     /locus_tag="ACGE36_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385019.1"
                     /translation="SESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFINKPDSL
                     FIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            275..517
                     /locus_tag="ACGE36_002676"
     CDS             275..517
                     /locus_tag="ACGE36_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFF8385020.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            535..837
                     /locus_tag="ACGE36_002677"
     CDS             535..837
                     /locus_tag="ACGE36_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385021.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            909..>2368
                     /locus_tag="ACGE36_002678"
     CDS             909..>2368
                     /locus_tag="ACGE36_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385022.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQ"
BASE COUNT          937 a          327 c          497 g          607 t
ORIGIN      
        1 attctgaaag tgtatggcat ccagatcctg tacaaataac agttaagagg caaaaagact
       61 atgaaggttt agaaagatac aaaatagtag attctgcagg cattagggaa tttataaaca
      121 aacctgattc attatttatt gttgttccct atgagaagga tggcgtaaaa aaaataattt
      181 ttcttggatc tagcaaggat gagaagcgaa cgaaagagaa attaattcat gatggatcag
      241 agttaaataa atacaaatta caaaagacta gtgagtgatt gctagtcttt ttatttatcc
      301 tgaaggagga atcacagtgg caaaagttaa aacaccattc gaaaaagccg cgtctaaaat
      361 cttagaacgc cgtggtgttt ctgaaataca gtggaaaaaa gaagttcttg aaaaagcaca
      421 atttgaccta ttacgaggaa aagataagga cttagaagaa tatatctatc aacgtgaacg
      481 tgaaaaactt gtattcgatg aaattattaa acattaaaag gagcgaataa tcttatgttt
      541 gaatcaatta caatgaattt tttaactcaa ccgaaagtag tagctgcagt tgatattttt
      601 agagcattac aaactgccgc aactagtttg aaaaataatc tgatgatttt tgctgctgtt
      661 gcggcagtgg cttccattgc tcttggaggt attttcttct tctttgggcg aagaggagct
      721 gaaaacggga agacacacgt aggttatgta ttgttggggg taggagttat tttagctgcc
      781 gcttctattg tagtttcaat gttctcaatg tttggacaaa ccccacaagg actataaaaa
      841 aaacaagtag gaggagtaaa aagtacgtgg ctacttttta ctcctttttt tatgaaagga
      901 gaatattcat gttttttacg atttcaggaa atgcaaaatt ggataaagag ttaataagaa
      961 aagcaacgaa gaaagtggga aagcgtactt cctttttacc gaaaacaatt aaatattctg
     1021 tcattgaaac ggaagaacag ctacatgtgt taggacaagt gttagaacaa gagaatcaat
     1081 cttctgttaa attgactctc acgttacatg ataaagaagg aaaaatagaa aaggctggca
     1141 cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttttat cctacattga
     1201 tagatttaat ccgccttgat gtgtttaaag atacggatga aactatgagt gtggaagagt
     1261 cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc gaccaaagaa
     1321 aagtgaatca agtgagtgaa gtcgaagtaa atgaagaagt accatgggaa cgaccaatct
     1381 ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa gctgatattc
     1441 cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg gaggtagatc
     1501 cctttgaaga aacaatggag gatgaagaga cggacgaagt agaagttgat ccagttgaag
     1561 ctaatgaaga gtcaacatgg actgaagggg attttcagga atatattgaa ccacttatca
     1621 gtgaagaagt agaaaaaaac gaaacagaga tacaaaaggc aactgtattt gattatattt
     1681 tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta tttttaaatg
     1741 agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag agtaaagaag
     1801 aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc caatcaacaa
     1861 attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt caacaagaaa
     1921 cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag aaagtaaaag
     1981 aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa gtcaaagagc
     2041 ggtacgaaaa gaaacgcgac aatgcttatt ccttgtgtgt agaaaagatc aacaatttta
     2101 ctaagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtatta
     2161 aagaacgaaa agaagagttc taccgcggtt tacgtacaga taagctaaag attagcaaag
     2221 aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt atggaaaaac
     2281 acgaacagat ccagcaagaa ttacaagtac aaattccaaa atggaaaaaa gagtatgaaa
     2341 ttcaacaaca agaattgaaa gaacaaga
//
LOCUS       DAXWLH010000241         2361 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXWLH010000241 DAXWLH010000000
VERSION     DAXWLH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.247"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            182..2179
                     /gene="tkt"
                     /locus_tag="ACGE36_002679"
     CDS             182..2179
                     /gene="tkt"
                     /locus_tag="ACGE36_002679"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFF8385023.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          782 a          433 c          529 g          617 t
ORIGIN      
        1 agggcaatga cgtgaccccg gaaaaattaa aagaaatcca aaagaaaaaa ggcttacaca
       61 accgaaatga caacttttag taaaaaatac tgaaaaagat tgctttttcg tgtgacatct
      121 tatagaatga aagtgtaaga aataaaggta aaaaaaacct aaattaatag gaggggttat
      181 tttgtttgac aaaattgacc aacttggcgt taacacaatt cgtacattaa gtattgaagc
      241 agtccaaaaa gcaaactcag gacatcctgg attgccgatg ggcgcagcac ctatggcata
      301 tgcgttatgg acaaaacacc tgaaagtaaa tccaacgaca tcaaaaaact gggctgaccg
      361 cgaccgtttc gttctttcgg caggacatgg gtcagcaatg ttgtatagct tgcttcattt
      421 agctggctat caagtgacaa tcgatgattt gaaacaattc cgtcaatggg attctaaaac
      481 accaggacat cctgaagtac atcatacaga tggtgtagaa gccactacag ggccattagg
      541 acaagggatt gcaatggctg tcggtatggc aatggcagaa gcgcaccttg cagcaacgta
      601 taacaaagaa aactttaatg tgatggatca ctatacgtat gcaatatgcg gtgacggaga
      661 cttgatggaa ggggtatctc aagaagcaag ttcgatggca ggtcatatga aactagggaa
      721 attgatcgtt ttatacgatt caaacgatat ttcactagac ggtccaactt caaaagcttt
      781 cacagaaaac gttggtgctc gctatgaagc atatggctgg caacatatct tagtgaaaga
      841 cggaaatgac ctagaagcaa tctctaaagc gattgaagaa gcaaaagctg aaacagacaa
      901 accaacattg atcgaagtaa aaacagtcat cggctatggt gctcctaaag aaggaacatc
      961 tgctgttcat ggtgctcctc ttggtgaaga tggcatcaag atggctaaag aagtttatgg
     1021 ttgggagtat ccagatttca ctgttccaga agaagtagct gcccgcttcc atcaaacgat
     1081 gatagaagaa ggacaaaaag ctgaagatgc atggaatgaa atgtttgcca attataaaaa
     1141 agcttatcct gaattagcac aacaatttga agatgctttt gatggcaaac taccagaaaa
     1201 ctgggatgca gaattgccaa cttatgaagt tggaagcagt caagcaagcc gtgtttcaag
     1261 taaagaagtc atccaagaat tatcgaaagc tataccaagc ttctggggtg gttcagcgga
     1321 cttgtcaggt tccaataata caatggtggc tgcagataaa gatttcactc ctgaacatta
     1381 tgaaggacgc aacatctggt tcggtgtccg cgaatttgca atggcttcgg ctatgaatgg
     1441 tatccaactt catggcggaa ctcgtatcta cggtggaaca ttctttgtct ttgtcgatta
     1501 tctaagacca gcagttcgtt tagcagcaat ccaacatact cctgttgttt acgtcttaac
     1561 acatgactcg gttgctgtcg gagaagacgg accaacgcac gaaccgattg aacaactagc
     1621 atctgttcgc tgcatgcctg gtgttcaagt gattcgtccg gcagacggaa atgaaacacg
     1681 tgctgcttgg aaagtagcga tggagacaac agatgcgcct actatccttg tattgagtcg
     1741 tcaaaacctt ccagtattgc cttctacaaa agaagtagca gatgaaatgg tgaaaaaagg
     1801 cgcgtatgtt ctttctccat cacaaggaga aacaccagaa ggtattttga ttgcaacagg
     1861 ttctgaagtt gatttggctg tgaaagctca aaaagaattg gctgaaaaag gcaaagatgt
     1921 ttctgttgtt tctatgccaa gctttgacct ctttgaaaaa caatcttctg aatataaaga
     1981 aagtgtactt cctaaatcag tgaagaaacg tgtggctatc gaagctgctg catcatttgg
     2041 atgggaacgc tacgtaggaa cagagggcgc aacgattaca atcgatcatt tcggtgcatc
     2101 tgcccctggc actaaaattt tagaagaatt tggatttaca gtagaaaatg tcgtgaatac
     2161 gtacaatcaa ctatcataga tccaagcaat tgtttgactg ataagaacag ctgtctgcaa
     2221 gttggagaca gctgttcttt atgtgttaga agaataaatc aatattactt ttcgttttcg
     2281 gctatgttac atttaaatga caaaacaaag aattcttcgg attagtttgt gtttcttgag
     2341 aatattattt gtcaaattaa g
//
LOCUS       DAXWLH010000242         2347 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXWLH010000242 DAXWLH010000000
VERSION     DAXWLH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.248"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(281..478)
                     /locus_tag="ACGE36_002680"
     CDS             complement(281..478)
                     /locus_tag="ACGE36_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8385024.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
     gene            complement(833..1918)
                     /gene="xerS"
                     /locus_tag="ACGE36_002681"
     CDS             complement(833..1918)
                     /gene="xerS"
                     /locus_tag="ACGE36_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFF8385025.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            2267..2347
                     /locus_tag="ACGE36_002682"
                     /pseudo
     CDS             2267..2347
                     /locus_tag="ACGE36_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
BASE COUNT          765 a          346 c          374 g          862 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattttttct accgacataa
      241 acaaaaaaac cacctcatat gaggcggtta acgttcaaca ttattttacg atatttactg
      301 cttgaggacc acgttgtcct tcttcaatat cataagtaac tgcttggcct tcatctaaag
      361 acttaaagcc ttctccttgg attgctgaga aatgagcgaa tacgtcattt ccatcttcac
      421 cagtgatgaa accaaaacct ttatctgagt taaaccattt tactgtaccg ttattcatat
      481 atatttcctc ctgatacgta tataatacgt gttttattgc aattaatatt acttggtaaa
      541 aggagttttt atagtgtgaa tatatcgctc agattacgtt tcaaattaga ttacttattt
      601 actataacac ggatgtaatg ctaacacaag gataatcact cgatatacgg tttaaatatt
      661 attatttgtc aaattaagcg agacaaaact ttccatttac gtaattcaaa aaaacttcta
      721 atggtgtttg gtaatttagt aatataaaaa tgataatgaa gcttgccttt aaagttgggc
      781 ttttatctaa ttttagagac aggcttcatt attatactat tttttactac atttacaaag
      841 cgttcaaagc atcaattgta gctgcattgt ctatatgagt atataaagtt gttgtatttg
      901 tacttgtatg tcccaattgt tgggcagtta ataccaatga atttgtctgt tgatagagtc
      961 tagtagccaa agtatgacgt aatttatgtg gactgacttg gactctgaat ccttttgaat
     1021 attttgctac catccgttcg attgcaatac cggatatacg cttggcttct ccttgataaa
     1081 ttgctagaaa taaagctttt tcgttttctg ttactttgta tcgttctttc cgaatagcta
     1141 gataattagc taaatattcc attgcaaatg ctgctatatt tactgcgtct ctcttatttc
     1201 cttttctagt tactaccaca ttattctttt ctaaagatag gtcctctaaa ttaatattta
     1261 caacctcgga gagtcttaat ccagatccta aaatcaatgc attgatcgcc aggtctcttt
     1321 ctacatctcg ttgataatag atcaaagctc gtttgctcaa agttttttta tattcattgg
     1381 caatataatc tagataattt tgtgtatctt cgtctagaaa taattttttc tcgattgcgt
     1441 ttgcacgggc agctaacgta gttttatctg tgatagttgc tacttgatcc ataggattat
     1501 tttctaagta tggttttcct aaaggattat tggaactttt cgaaagatat ttaaatagaa
     1561 cctttaatgc tgtgatattt ctattgatag tagaagtaga tagacctaag ttattattat
     1621 ctttgagctt agtttttgtt gtctcttttt ttacattttc ctttgtgcga cgtttcaaat
     1681 gagatttgta caagtttaga tcataggcag ttaactgtct tatcgaggtg attgaagtat
     1741 cgggaacgat agactcactc agaagccatt cataaaagag cttataattt tttagatatt
     1801 ctaaagtagt tcgaggtgat tgatctttat cctcacgatc ttgaacaaaa tcttttatat
     1861 aatcgggcat atgaagtatg attgtatcta ttttttgata tatttctgta tttttcatgt
     1921 tattcatcct ttcgataagt aggggagaga agtatctcta taattggact ataccaatta
     1981 tagaatgttt tgtcaattgg tatactctga aatttattaa tttatttcaa aaaagcagat
     2041 aaataggtat agatttaata atttaaaata aatggtatac atgaatagga aaggaaacag
     2101 aaggaggcaa tcttaatttt ttaaattgtc taaattatat agaaagaaca atatttctat
     2161 ataattagac ataagagaga ctacggttga tttgatatta acaagcgtat atgaatgtat
     2221 atacaaataa aaaaggggag ggagaagtta aagaaaattc agagaagtgg ggcactcagt
     2281 agtattaaca atttaaaagg aatttcttaa agaaaatggg ttgcatcctg gagatacaat
     2341 tttttta
//
LOCUS       DAXWLH010000243         2342 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXWLH010000243 DAXWLH010000000
VERSION     DAXWLH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.249"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1164
                     /locus_tag="ACGE36_002683"
     CDS             <1..1164
                     /locus_tag="ACGE36_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFF8385026.1"
                     /translation="AVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQN
                     GSQLRTSLLTIAKEWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLA
                     KEGFSNMSIFQTAKGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDK
                     ADTMKVTTCLNPLHTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPG
                     IIDPKIFLEEVITVRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTL
                     VFIPLTLAAWLRYLIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAP
                     ILSNKEIFGVDLYQAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1155..2237
                     /gene="uxuA"
                     /locus_tag="ACGE36_002684"
     CDS             1155..2237
                     /gene="uxuA"
                     /locus_tag="ACGE36_002684"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8385027.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT          786 a          414 c          492 g          650 t
ORIGIN      
        1 gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg
       61 ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat
      121 ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa
      181 aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg
      241 tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg
      301 ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt
      361 gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat
      421 ggacgtccga aattcgaaaa agctggagta ttcttgactg atcgtgcaac ggtagataaa
      481 gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg
      541 ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg
      601 ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt
      661 gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct
      721 gatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag
      781 acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca
      841 ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc
      901 atcccgagtc cagacccact attgacagac tttcagcata agctttccga tattcaactg
      961 ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa
     1021 atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc
     1081 aagatggtag caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat
     1141 ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta
     1201 ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata
     1261 taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt
     1321 ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg
     1381 gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag
     1441 aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg
     1501 accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc
     1561 cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc
     1621 cgggatggga acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag
     1681 tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat
     1741 gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg
     1801 gattacctcg aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca
     1861 gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg
     1921 tatatgggat tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa
     1981 atattaaatt tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt
     2041 cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc
     2101 gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg
     2161 atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca
     2221 aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc
     2281 aaattcttgg agagtataaa atattttgtg taaatgaaaa aatccataca aaaaaggaag
     2341 tc
//
LOCUS       DAXWLH010000244         2338 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXWLH010000244 DAXWLH010000000
VERSION     DAXWLH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.250"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            296..508
                     /locus_tag="ACGE36_002685"
     CDS             296..508
                     /locus_tag="ACGE36_002685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFF8385028.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            510..2189
                     /locus_tag="ACGE36_002686"
     CDS             510..2189
                     /locus_tag="ACGE36_002686"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8385029.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT          746 a          447 c          480 g          665 t
ORIGIN      
        1 tatgattata gcgggatgat aaacattgat tttttctttc aatctagttg atttctatac
       61 ttatttgctg ttttatgcta gaataacaat taattgttgt atatttttta tacagaaggt
      121 acggttgaaa aaatatccga acagataagc aaaaacgagg cttgtctctt tgactcgaat
      181 ccagtattct agtcaaagca tatgaaaaac caggtggctt gtcccctcgt tttgtgttgt
      241 ctaaaaaaat caactagcta aatcactgct ttacgcagat aaaggagaca aaacaatgat
      301 ctataaagtt tattatcaag aaacaaaagt acgcaaccct aaacgggaag aaacaaaatc
      361 tctttacatt gaggcaaaaa gcgatgtaga cgctcgccaa cgtgtggaag aaaatacccc
      421 atacaatatt gaattcgttc aacttctaga aggaagtcat ctagagtacg aaaaagaaaa
      481 tgctgatttc tcattagtgg agttttaata tgaaactttc catcaaaaat aacgagacag
      541 gcgtctttgc tattggtgga ttaggtgaaa tcgggaaaaa tacgtatggg gtacaattcc
      601 aagatgaaat cattttgatt gatgccggga tcaaattccc ggaagacgat ttacttggaa
      661 ttgactatgt cattcccgac tacagctaca tcatacaaaa cttacacaaa gtaaaagcct
      721 tggtgattac tcacgggcac gaagaccaca tcggcggaat accctacttg ctgaaacaag
      781 caaatattcc tatctatgct ggtcctctcg cattagcgtt gatcacaaac aaattagatg
      841 agcatggctt gcttcgagat gcagagctcc atgagatcaa tgaagatacg atcatccgtt
      901 ttagaaagac ttctgtcagc tttttcagaa cgacccatag tatcccagat gcattaggga
      961 tcgtggtgaa aacaccttct ggaaatatcg tagcgactgg agacttcaaa ttcgatttca
     1021 ctcctgttgg cgaaccagca aacttgcata aaatggctcg tatcggggaa gaaggagtct
     1081 tgtgcctttt atctgatagt acaaatgctg agattccaac atttactaaa tcagaaaaaa
     1141 cgattgggca ttctattttg aagatctttt caaaaatcga aggacgtatc atttttgcca
     1201 gctttgcttc taacatcttc cgtttgcagc aagcagcaga tgccgctgtg gcaactggtc
     1261 gtaaaatcgc tgttttcgga cgaagcatgg aaaatgccat cgtcaacggt caacgtttag
     1321 gatatatcaa agttccagac ggcacatttg tagatgcaca cgaaatcaat cgcttgcctg
     1381 ccaatgaaac gatgatccta tgtacaggtt ctcaaggaga agcgatggcc gcgttgagcc
     1441 gcatcgcgaa tggtactcac cgtcaaatcc aaatccagcc agacgataca gtgatctttt
     1501 caagttcacc tattcctggt aatacaacaa gtgtcaatca tttgatcaat ttgctgtctg
     1561 aagctggtgc agaagtgatt cacggaaaaa tcaataacat ccatacttcc ggtcacggtg
     1621 gacaagaaga acaaaaatta atgcttcgtt tgatgaagcc aaaatatttt attcctgtcc
     1681 acggagaatt ccgcatgttg aaagtacatg cttctctagc acaagatgta ggtgttcctg
     1741 cagaaaactg cttcatcatg gaaaatggcg atgttttagc gttaactagc gattctgcgc
     1801 gtcctgctgg gcattttgtg gctggagatg tgtatatcga tggtagcggg atcggtgata
     1861 ttggtaatgt cgttttacgt gaccgtcacc tgctttcaga agaaggtctt gtattagctg
     1921 tcgcgacagt ggatatcaag aaaaaagaaa tcttggctgg tccagatatt ctctctcgtg
     1981 gatttgtcta catgcgagaa tcaggagaaa tgatcaatga agctcagcga atcttattcc
     2041 acgctttaag agaatgtttg aatggacctg actgttcaga atataaattg aaagaagcga
     2101 tgactggcgc gcttcagcct ttcttattcg aacgtacaga aagacatcca atgattttgc
     2161 caatggtaat gacaccagat gaagaataaa taagaggtta tgaaatcagc accttgtcct
     2221 gaatataaga gaaaacaaat gaaaaacatg agaagccatt tttgtttgtt cttattcctt
     2281 gatacaaggt ggctctttca tagcctctta aatttattta ctttttcata caaaaaaa
//
LOCUS       DAXWLH010000245         2332 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXWLH010000245 DAXWLH010000000
VERSION     DAXWLH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.251"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(138..428)
                     /locus_tag="ACGE36_002687"
     CDS             complement(138..428)
                     /locus_tag="ACGE36_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385030.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACGE36_002688"
     CDS             complement(546..806)
                     /locus_tag="ACGE36_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385031.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACGE36_002689"
     CDS             complement(943..1356)
                     /locus_tag="ACGE36_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFF8385032.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACGE36_002690"
     CDS             complement(1812..2297)
                     /locus_tag="ACGE36_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385033.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          852 a          378 c          368 g          734 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc ag
//
LOCUS       DAXWLH010000246         2312 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXWLH010000246 DAXWLH010000000
VERSION     DAXWLH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.252"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(693..1796)
                     /locus_tag="ACGE36_002691"
     CDS             complement(693..1796)
                     /locus_tag="ACGE36_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFF8385034.1"
                     /translation="MANKNGYDIEMSYVVAPGETLKEVAEELGLSHKDLAERLGITPK
                     TISKIVNGTAPITPETALKLERVLGISSNFWNQLELNYREKLTEIEAEAKLKDEIEIL
                     KEYIPYNEIANAGFVPKTRNLKEKVENTLNYFGYSSLKIFIESVNDNQLLAGAYRIKE
                     VDKVNKLALLAWIRAGELEAQEIEAEIFSKEKLKNSLSELRNLTLQSNPSIFIPKLQK
                     ICASFGVKVVFIPEVKGSKVCGLTRWLTPKPQAIIQLSLRYKSNDNLWFTFFHELGHI
                     LKHNKIPFYTSSNEYIDSIEEQEADEFSANTLIPRDSYNDFVEQGCFTRNSICNFAEM
                     ISIHPGIVLGRLQKEKYVQWNQYNDLKTWYSWK"
     gene            complement(1786..2115)
                     /locus_tag="ACGE36_002692"
     CDS             complement(1786..2115)
                     /locus_tag="ACGE36_002692"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8385035.1"
                     /translation="MIVRARKRKLQKILADSKLINLEFGKQIGDKVKQRIAELLAAET
                     LEDISHLPPTKLHSLSGEYEGCFAVKLTGNFRLVFKAYTCEEEFTTQKSNTEIIVIEE
                     VVDYHGK"
BASE COUNT          718 a          432 c          323 g          839 t
ORIGIN      
        1 ccaattataa gtctttttcc cattattttt taaagttata tggtatgctt ttaatagctt
       61 caaatataaa agagtttaaa gcgtaacaca cttatggggg agtggttttt ggggaacgct
      121 ttaaactctt ctttattatc tcacaatata acccaaatgt ctttctattt taaaagtcaa
      181 agtaaaactt tttaaatata cagaagtata actatgtgaa acatcctttc attaacccat
      241 aaaaggatac ataaaaaaag ccactcattt gagtggcaat gaagaaaagc tttagcttgt
      301 ataatactct tcaaaaaatt ctaacacaga acaattcaat tggctacatt aatgtaccct
      361 gtaggactcg agcctacgac cggacggtta tgagccgtct gctctaacca actgttaatc
      421 agtgttccaa agggagtgat tatactctgc tattttcaaa ctttcttaaa tcgtctatga
      481 taatctcagt ttatggtggc aaaatggtgg caagaaaaaa agtgagtatg cccgctactt
      541 gcgcaatcgg agtactgctc gatcatttat ttggaaacag gctacacttg agggtttgga
      601 agagtcaaag cggttgagcg gtgaacggaa aaagaactta actacgtata gttaggttct
      661 tttcgtttat gttttatcgg tagaataatt ttttacttcc aagaatacca agtcttcaaa
      721 tcattatatt gattccattg tacatacttt tctttttgca accttcctaa aacgatcccc
      781 ggatgaattg agatcatttc cgcaaaattg caaattgaat ttctagtgaa acaaccttgt
      841 tcaacaaaat cattatagct atctctaggt attaatgtat tagcagaaaa ttcatctgct
      901 tcctgttctt ctatactatc aatatactca tttgaactag tataaaaagg aattttatta
      961 tgctttaata tgtggcctaa ctcatggaaa aatgtaaacc ataaattatc attagatttg
     1021 tatcttaaac taagttgtat tatagcttgc ggtttggggg ttagccacct tgttaaccca
     1081 cacactttac tcccttttac ctctggaatg aatactacct tcactccaaa actagcacaa
     1141 atcttctgta atttaggaat aaatatacta ggattggatt gcaaagtaag atttctcaat
     1201 tcagataaag aattttttaa cttttcttta gaaaaaattt ctgcctcaat ctcttgagct
     1261 tctaattctc cagctctaat ccacgccaac aaagcaagct tatttacttt atctacttct
     1321 ttaattcgat acgctcctgc taacaattga ttatcgttaa cactttcaat aaagatcttt
     1381 aatgaagaat acccaaaata attaagcgta ttttcgactt tttccttaag gttcctagtt
     1441 ttaggaacaa atcctgcatt tgctatttca ttatatggta tatattcttt caaaatttcg
     1501 atttcatctt tcaacttggc ttctgcttca atttctgtaa gcttctctct gtagttaagc
     1561 tccaattgat tccaaaaatt tgaagatatg cctaaaactc tttctaattt taaggctgtt
     1621 tcaggtgtaa ttggcgcagt tccgttaacg attttagaaa tagttttcgg tgtaatccct
     1681 aacctctcag ctaaatcttt atgagataac ccgagttctt cagctacttc tttcaatgtt
     1741 tctcctggag caactacata ggacatttct atatcgtacc catttttatt tgccatgata
     1801 atccaccacc tcttcaatta ctattatttc tgtattactt ttttgtgttg taaattcttc
     1861 ttcacatgta taagccttaa aaactagcct aaaattaccc gtcaatttta cagcaaaaca
     1921 cccctcatat tccccagaga gagaatggag tttcgtgggc ggaagatgcg aaatatcttc
     1981 taatgtttct gccgctagca attcagcaat tctttgctta actttatctc caatttgttt
     2041 tccaaattct aaattaatta gcttggaatc agctaatatt ttctgtaatt ttcttttcct
     2101 cgctctaact atcatccgac ttaacctttt aggttaacaa ctctaaatat agtataccac
     2161 aataatttgt ttttcaagca ctttatgatt tacttaaaga ttaacctaaa ttctttcgct
     2221 tatagtagtt tctctttata gattatcaaa agttgtaaag attttaaagt caattttgta
     2281 taaaaaaaac ttttttcgtt ctatgtactg cc
//
LOCUS       DAXWLH010000247         2304 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXWLH010000247 DAXWLH010000000
VERSION     DAXWLH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.253"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            474..1085
                     /locus_tag="ACGE36_002693"
     CDS             474..1085
                     /locus_tag="ACGE36_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385036.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFNRVGVEKVFAEKKSGRNTAERP
                     EFNKMLAFVREGDTLVFESLERLGRNSDEILDTVSFIDKNGMSLMVLNLPILDTKFGD
                     PNLEKLVRSLVISIFSWTAQNEREEIKRKQKQGIAIAKRKGYYKGKPYEYSENAKNPQ
                     KRFIYNEIVRMLGEGLPIKKIAEKTGTNRNLVYRIKKRISESG"
     gene            1329..1559
                     /locus_tag="ACGE36_002694"
     CDS             1329..1559
                     /locus_tag="ACGE36_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385037.1"
                     /translation="MNQPRRKRLESISEKISELKDLLEEIKEEEEEYKENMPENLWGS
                     ERYERAEEAVGQLEEADDYLDQVIGLIEEVIS"
     gene            1613..1966
                     /locus_tag="ACGE36_002695"
     CDS             1613..1966
                     /locus_tag="ACGE36_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385038.1"
                     /translation="MTQNYIDNWRLFSGKLMTKDSIAEMPESLEKEHILKVREMLHKE
                     GFNADLFRVGELPIEGVYYFERAVSENGVDYFSIIVEDDGSLTPQYTTLECDESGFNT
                     FPCNTIQESIDKHEC"
     gene            1994..2302
                     /locus_tag="ACGE36_002696"
     CDS             1994..2302
                     /locus_tag="ACGE36_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385039.1"
                     /translation="MKLEIKEVVCDWGIYVDGQTYPFMIFNSKANAQEIMRIMELDNK
                     HERFDEVNKMENECEAVEYHKTCANNKYILGAMGLGKTASIKQVYEESQENCVYENSN
                     "
BASE COUNT          910 a          264 c          491 g          639 t
ORIGIN      
        1 ctccactcac tgaagggtca actccgtcat attgtgtgac cagacggaag gacgaatatt
       61 gttgaggttt tagaaccatg taaaacaaca aagttctcaa atagaataag ttcaaagaag
      121 gtttagatga tggttttaga accatgtaaa acaacaaagt tctcaaatgt gtagataaat
      181 aatttttaaa tattaatggg ttagcatatc ttttaggtct tagttacatg ctaagactat
      241 ttttttggtt tatatgttac gtaagggtat accctattgt aacactagtg gaaatgcgat
      301 ataataggaa ttagaggtgt gacacaaggg tgtgacataa aggggtggat atactgaaga
      361 cagaaaagaa aaaattgtag caaccaaaaa agaaaaaatg agagttgata aactcacgaa
      421 aaaattccta gtttgtttga ctagagtaaa ttaagttatt tgggtgtgaa aatatgttag
      481 taggatatgc aagagtaagt tcagacgatc aaaacctaga acgacaactt gaagaattta
      541 atcgtgtggg tgtcgaaaaa gtatttgctg aaaaaaaatc aggtcggaat acagccgaac
      601 ggccagagtt taataagatg ttagcgtttg ttagagaagg agatactctt gtctttgaat
      661 ctcttgaacg attaggaaga aattctgatg agatattgga tactgtatcg tttatagata
      721 aaaatggtat gagtcttatg gttttgaatt tgccaattct agatacaaag tttggtgatc
      781 ctaacttaga aaagcttgtc cgaagtttgg ttattagtat tttttcttgg acggcacaaa
      841 atgaaagaga agaaataaag agaaaacaaa aacaaggtat agcaattgcg aaaaggaagg
      901 gatattacaa aggaaaacct tatgagtatt cagaaaatgc caaaaatcca caaaaaagat
      961 ttatttataa cgagattgtt cgaatgttag gtgaaggatt accaatcaaa aaaattgcag
     1021 aaaaaacagg aacaaataga aatttagttt atagaataaa gaaaagaata agtgaatctg
     1081 gttgatctta agttctttgt taaatcaacg ttacgagagg aggattctaa gcctgtttca
     1141 aacttttaag atgtacaact atacaaagaa gcttctaaat aaaagaggtt atattgggga
     1201 atctctaata tagatcattg actggaagga tagtttatat attcaaagaa gggtgttgaa
     1261 aaaaaaagga agaaaaaact aataatttgg atttttaaag ataatacagt caaaaggagt
     1321 ttttaaaaat gaatcaacct agaagaaaaa ggttagaaag tatttctgaa aaaatttccg
     1381 agttgaaaga tttgcttgag gaaattaaag aggaagaaga agaatacaaa gagaatatgc
     1441 cagaaaactt atgggggtca gaacgctatg aaagagccga ggaagccgta gggcaattag
     1501 aagaagctga cgattatttg gatcaagtaa ttggattaat cgaggaagta atcagctaaa
     1561 taccaagcta gcaaaagaaa tataatcaaa attaaataaa ggggaaataa caatgactca
     1621 aaactatatt gataactgga gattattttc tggaaaatta atgacaaaag acagtattgc
     1681 agaaatgcca gaatcattgg aaaaggaaca tattcttaaa gtccgagaaa tgttgcacaa
     1741 ggaagggttt aatgcagatt tattccgagt tggggaactt ccaattgaag gagtttatta
     1801 ttttgagcga gctgtatcgg agaacggagt cgattatttt tcaataattg ttgaagacga
     1861 tggttctctt acgccacagt atacaacgtt agaatgcgac gaaagcggat ttaatacttt
     1921 tccttgcaat accattcaag aatcaattga taagcatgag tgctaattaa ttatctacag
     1981 gaaggtgttt tttatgaaat tagagataaa agaagttgtc tgcgattggg gaatctatgt
     2041 agacggacaa acatatccat ttatgatttt taatagtaaa gcaaatgcac aagagataat
     2101 gcgtatcatg gagttggata ataagcacga aagatttgat gaagtaaaca aaatggagaa
     2161 cgaatgtgaa gcagtggaat accataaaac atgcgccaat aataagtata ttttaggtgc
     2221 aatggggtta ggtaaaacag cttctattaa acaggtctac gaagaatcac aggagaattg
     2281 cgtatatgaa aattcgaatt aaag
//
LOCUS       DAXWLH010000248         2291 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXWLH010000248 DAXWLH010000000
VERSION     DAXWLH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.254"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..343
                     /locus_tag="ACGE36_002697"
     CDS             <1..343
                     /locus_tag="ACGE36_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFF8385040.1"
                     /translation="LKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQT
                     GQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMP
                     IFLMPANEKNN"
     gene            380..1390
                     /locus_tag="ACGE36_002698"
     CDS             380..1390
                     /locus_tag="ACGE36_002698"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFF8385041.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1505..2218)
                     /locus_tag="ACGE36_002699"
     CDS             complement(1505..2218)
                     /locus_tag="ACGE36_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385042.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          762 a          384 c          472 g          673 t
ORIGIN      
        1 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
       61 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
      121 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
      181 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
      241 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
      301 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
      361 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
      421 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
      481 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
      541 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
      601 tgaaaatatc caaatgatct ttccgccttg gggaggagaa ttattgattg agattctcga
      661 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
      721 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ccaaccttat
      781 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
      841 aagagaagga gaatcgattc tacagcaacc ttctgaaaag tatcaaaaag agtggcccca
      901 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
      961 cggagatgca gtaaggatga agggacgatt attaggtggc tgtattgata cgatcagtca
     1021 tttaatcggt accccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1081 gccgatcttg tggtttctag aaaattgtga gctatcgtca gttgatttaa gaagatcact
     1141 tgttcagatg aaactggcag gatggtttga tggcagctcg gggatcatgt tcggtagaag
     1201 tgcagcagag gatttgttag attataaaat gaaggatgta tatgaagatc tgtcaaagga
     1261 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     1321 gatcaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     1381 gtttatttaa aaaatttttt cctgccttgg agtttatcaa agaccaacaa atctaaatag
     1441 aaaagaaaat gaaagaagct gacagtattt tgccagcttc tttttgtatg atctattaga
     1501 aattttaatt atccataact atcacattga tcagctgttt cacatcattt ggattacctg
     1561 taggaatagc tactacagat gcattatcaa taatttcttg agttgtttct ctaatgaacg
     1621 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     1681 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     1741 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     1801 ctgcaggata atgaataaca tgtagttcga aatcacccga tgtatctcta aaagtattac
     1861 ctctaattgc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     1921 gatctattga ctgagaagac tgtgaggaag ttgtgtctat tgttgaagaa gaaatatttg
     1981 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     2041 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     2101 tttgtttgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     2161 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     2221 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     2281 aaaccttgtc t
//
LOCUS       DAXWLH010000249         2281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXWLH010000249 DAXWLH010000000
VERSION     DAXWLH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.255"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..421
                     /locus_tag="ACGE36_002700"
     CDS             <1..421
                     /locus_tag="ACGE36_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8385043.1"
                     /translation="LISGKYDYAFDFEGEKDYKSVAVFQSDFEKLKNYAGRRNVNFAR
                     LLEEFYSEELGVGNEYKRETYAKLSHKLSQALKEVTEVKAELDAKLEEVKKYQTERKN
                     NMKNQVEFDEQKEFEKARKRYNKIMNELSYEHLTVHE"
     gene            422..625
                     /locus_tag="ACGE36_002701"
     CDS             422..625
                     /locus_tag="ACGE36_002701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385044.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            645..2273
                     /locus_tag="ACGE36_002702"
     CDS             645..2273
                     /locus_tag="ACGE36_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385045.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
BASE COUNT          911 a          267 c          432 g          671 t
ORIGIN      
        1 attgatttca ggaaagtatg actatgcttt tgattttgag ggtgaaaagg attacaaaag
       61 tgttgctgtc tttcaatctg attttgagaa gctaaaaaac tatgctggca ggagaaatgt
      121 taattttgca agactactag aagagttcta ctccgaagag ttgggagtag gaaatgaata
      181 caagagagaa acttatgcga aattgtctca taagttatcc caagcgctta aagaagtgac
      241 agaagttaaa gcggaactag atgctaaact tgaagaagtg aaaaaatatc aaacagagag
      301 gaagaacaac atgaagaatc aagtggaatt tgacgaacag aaagaatttg aaaaagcaag
      361 aaaacgctat aacaaaatta tgaatgaact tagctatgaa cacttaacag ttcatgaata
      421 attgagtgag ggtacagaaa aatatacgat cgcagatatg gttaatgaag tggaaactct
      481 tttagcttct ttctttgaaa atggtcacgt aaacgatgaa tggcgaaaag aagaacctaa
      541 aagggttaac gcttatattg ctcgattaaa aaggtttatt gataaatata aggagaatag
      601 tgacaaattt tctaaagata aataaggaga gagtgtgatt acatttgcca gaaatcgaag
      661 gaatattttc gccaaaaata ggtaaaaaac agttagattt cagcagtttt ccgagcgtaa
      721 atagagatta taatctaggt gatgaggtac agatactaaa tattgatttc aaagaactag
      781 acttcgctgt tgctgtttcc aacaaaccca aggcattgga acgagtgaaa aaaatatacc
      841 aagagaatga tcaaaatatc aaaaatatga tcaaaaagta tagacgagat aattcaaaag
      901 ttgaagacgt tttattcaac ggtacggcaa aacatgattt gaatgtttat atggcattag
      961 gagtggcttt gtactgtaat tcaattaatc agacagatat tttggttaat atcgtgagga
     1021 atagctatca aaaaatatta aaaataagta gcgaaacaaa cccttattat gcaatgaaat
     1081 atcttgggga taatttatta aatgataaaa aattgatgca caacactgaa gagctgttat
     1141 ctaatttggc tgcgattaac tttctattca aaacaggttc tgaagattat tttatagata
     1201 acgtactaaa aggtgcttac ggaacagcat tttatccgct ttacaatatg aaacagcata
     1261 aagccgaaca aaaggataac aaacttgaaa ttaaaaagat gaaagaacta ttaggtatag
     1321 gatttgtaaa acccaattca aaagtcacta ttgcttcgtt agcaactact aacaatgatg
     1381 ggaaaatagt aaatgggttt gacgattcag atatagatat attgtcaaaa tcatattttt
     1441 ctttgtatgg agattttgca gatttgatgg gtattgatag ttacttagtc aataattttg
     1501 agatcaaggg tagcgagctg aatgatattg tgtatttcgt tttaatctct ctatatgcaa
     1561 ttgatcagaa taccgagtac cgacaaattg actatttgct agttttttat agtttgttat
     1621 atcagttcgt tttaactcgg ttgtttaaaa atgctaagga agaattgatc ctgaattctg
     1681 attatgaaat agcaaaacaa attgcaagta ttagaaaaga aaacaaatct gaagtaatca
     1741 aattaagaaa agaattaagt attgcgagta aagaaaacgg tcggttagaa tctttagtca
     1801 aatcggcaaa aaaagaagag gaacttttga agcaagagat taaagaatta aaaggaatca
     1861 gaaaagaact tgaacagctg aggtttgaaa ataaagtatt aaagcaattg caggagcaag
     1921 aacatagaca atcattaaat ggtaaaactg aagagccttc actgtctgaa atgatagctt
     1981 atctaaaaca taaaaacatt cttttagtgg gaggacatgt aaattttcaa aataaattaa
     2041 ctgaactatt accaaatatt aaaatgtttg atgtaagcga aaataatgga aagatactta
     2101 aaaatttgaa tacgtgtgat gcgatttttc tatatacaga ttacttgaat catggtctat
     2161 attatcgtgt tatgagtgaa atttctaata aagataagat attttatttg gataatcatg
     2221 tgaatatgaa cttaacaatt aaacgtattt acgatctttt atcacttata taaagagaga
     2281 a
//
LOCUS       DAXWLH010000250         2271 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXWLH010000250 DAXWLH010000000
VERSION     DAXWLH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.256"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            64..348
                     /gene="groES"
                     /locus_tag="ACGE36_002703"
     CDS             64..348
                     /gene="groES"
                     /locus_tag="ACGE36_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFF8385046.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            399..2024
                     /gene="groL"
                     /locus_tag="ACGE36_002704"
     CDS             399..2024
                     /gene="groL"
                     /locus_tag="ACGE36_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFF8385047.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT          785 a          379 c          523 g          584 t
ORIGIN      
        1 ggttagcact cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt
       61 atcgtgttaa aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa
      121 actgtcggag gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt
      181 gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa
      241 gcaggcgacc aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa
      301 gaatatctga tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata
      361 aaaaaataat caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg
      421 cagaagacgc acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag
      481 taacattagg acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga
      541 tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca
      601 tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta
      661 caacgacagc aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg
      721 caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg
      781 aagaattgca taatatctca actgttgttg attcaaaaga agccattgca caagtagcag
      841 ctgtttcttc gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg
      901 gaaacgacgg tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg
      961 tagaaggtat gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca
     1021 aaatggaagc tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca
     1081 tccaagatat tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta
     1141 ttgctgacga tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa
     1201 cattcaacgt agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg
     1261 aagatatcgc tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga
     1321 aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata
     1381 caacgatcgt agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca
     1441 aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag
     1501 ctaaattagc aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga
     1561 aagaattaaa attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag
     1621 gaatggtctc aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag
     1681 aagcagaagg agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa
     1741 ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga
     1801 acgtggaact aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag
     1861 caggtatcgt agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt
     1921 ctgccttgtt attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag
     1981 cagctcctgc aatggatcca tcaatgatgg gcggtatgat gtaagaaaaa gcaacttgct
     2041 tatctacaac tgatcattag tgaaagacag ataaaaagag gctggaacag aagcgtttaa
     2101 ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca aatttttgtg aatttcagct
     2161 tatttccgaa ggagttgctt ctgcttccgc tgtttatacg tgtttagagc gtgagacaaa
     2221 agtgttcttt acttttgtcc cacgctcttt tttgtgtgta attataatga a
//
LOCUS       DAXWLH010000251         2270 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXWLH010000251 DAXWLH010000000
VERSION     DAXWLH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.257"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            191..445
                     /locus_tag="ACGE36_002705"
     CDS             191..445
                     /locus_tag="ACGE36_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8385048.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACGE36_002706"
     CDS             446..763
                     /locus_tag="ACGE36_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8385049.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACGE36_002707"
     CDS             1322..2257
                     /locus_tag="ACGE36_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385050.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DAXWLH010000252         2253 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXWLH010000252 DAXWLH010000000
VERSION     DAXWLH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.258"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            90..1703
                     /locus_tag="ACGE36_002708"
     CDS             90..1703
                     /locus_tag="ACGE36_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8385051.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          707 a          400 c          444 g          702 t
ORIGIN      
        1 attcaaaata aacaataatt ttccggcaca attatttgct gttttttacc ataacatagt
       61 acgatcaacg tagaaaagga gatgttttta tgaaagttgt tgtcattggc ggtgtagctg
      121 gcggtccttc atttgccact cgtttccgtc gattgaatga agcgcacgaa atcattatct
      181 atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt gccttattat ttaggaggtg
      241 tgatcactga ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca
      301 acatcgatgt ctttactaaa cacgaagtaa cagcaatcga tccttctact aagcgattaa
      361 cagttaaaga cctatccaca aatgaagaaa taaaaacaga ttacgataag ttgatcatct
      421 cttctggtgc tagaccagat tatccggata ttcccggagt ttttgaagca gaaaacggct
      481 ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc
      541 cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct ggaattagct gagaatttca
      601 agcatcgcgg cttagacgtg actttaatcg atcaattgcc acaagtcgct ttcccttatg
      661 atccagaaat tgctaattta gtttatgaca aattgctgaa agaaggatta gccgttcatt
      721 tagaaacaag agttactgag atccgtgata aaggtcgaga aatagtatta tcagatggct
      781 ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg
      841 tgaaagcaac cggcatacaa ttatcagata caggtcagat cattgttgat gaccagttac
      901 aaaccaatct gccggacatc tatgcgattg gcgatattat cgaaacgact agtgtagtga
      961 ctggtcagcc gatccaaagt atgctttcca gtgcggccaa tcgtcaagga catatgctgg
     1021 cagatatttt aaatggtacg cctatgcgct atcgcggata tattggtgca ggtgtcgcaa
     1081 aaatctttga tcatacagca agttatgcgg gaatgacaga acatgaatta aaagcatcag
     1141 gcataacaaa ttataaaact gtttttatca ctccctttga ccatgcctat ttctatccag
     1201 aagctacaag attaaatcta aagctgattt ttgatgcaga tagcggtcgt attttaggtg
     1261 gacaagcatt tggagaaaaa ggtgtcgata aacggatggg agaactttct gtagcaatca
     1321 ccggaaactt gacagtcttt gatttgcctg atttggagtt gccttactct ccaccgtatt
     1381 ctactacccg tgacccgttg aatatagctg gttatgtcgc aatcaatcaa atgacgaata
     1441 tcgtagaaac gatcaaagca agtgatatac ccgaaaacga tttgaaagaa gcgttctttt
     1501 tagatatacg tgaacctaat aaagcacctt ctggcagtat ctcggcaaca aaaaatattc
     1561 caatgaatga gttacgagat cgaatcaatg aaatcccaaa agataaaaaa gtttacatta
     1621 ctttcagaag aggattgaat acttatactt ctgcccgaat cttggcaggt ttgggtatca
     1681 aagcgatttt gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt
     1741 atttttactt gaggagtaaa aatgaaaaag tatatagggt tgttggttgg tatgtatata
     1801 gaatagaaga taaatgtgaa gaaattgtgc attatttctt cacatttatt aggttatagt
     1861 ttaagaaagt ttaaacattg cgcaatttaa ttattttagc tgtttctttt cataaaaaaa
     1921 catgttgtga ctctgagaca aatcatagtc catagtgatg tctctagagt aactacgcct
     1981 aatattacga ggcatcatat taatctatca gcactagtac ctaaactagc atccctttcg
     2041 aatatttttt cttatataat aaagtttgct ttcactcctc taatctcacc aaatcgactg
     2101 gcttgcgttg tgtgatacta ggaactgatt ggtttagaac gacttttcta ttgtaatcat
     2161 atttttcgcc atcctcgttg tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt
     2221 aaataattta atttttttgt tcctatttat tta
//
LOCUS       DAXWLH010000253         2245 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXWLH010000253 DAXWLH010000000
VERSION     DAXWLH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.259"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            3..1085
                     /locus_tag="ACGE36_002709"
     CDS             3..1085
                     /locus_tag="ACGE36_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8385052.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            1243..>2245
                     /locus_tag="ACGE36_002710"
     CDS             1243..>2245
                     /locus_tag="ACGE36_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFF8385053.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     "
BASE COUNT          780 a          402 c          435 g          628 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaaccggct ctataattta tgggactgat gaatcagaat
     1141 tgattcttca gccccatttt tattttaggt attaaaaaac atatcgttct ctagtatgat
     1201 taaatcacga caaaataaac aactgagagg acgatatgct atatgaatga ttctatcaaa
     1261 aaaatgctgc gattaataga gaaagatttg atgattacag aggtctctta cgagaccttt
     1321 cagaagaaaa agacgttgat cgtcgatgct gttttctcac ctgctcccca tacctgtaga
     1381 aattgtggtt ctactgtggt agatggaaac gggaaagtaa ttgtagtgaa aaatgggaag
     1441 aaggaaacga ttgtccgctt tgaacaatac aatcacatgc ctttggttat gcgcttaaaa
     1501 aagcagcgct atacctgtaa aaactgtcga acccattgga cggctcaaag ttattttgtt
     1561 caacccagac attcaatcgc gaatcatgtc agatataaaa ttgcttcttt actgactgaa
     1621 aaagtatctt tatcttttat tgcgaaaaat tgtcaggtat ctttgaccac cgttattcgt
     1681 acgttgaaag agtttaaaag ctacttacca aagcaatcta agaggattct ccccagagta
     1741 ttgatggttg atgaatttcg ttcgcacgct tccatagaag ataaaatgag ctttatttgc
     1801 gcagatggcg aaacaggaca attaattgat gtcttgccta cgcgtaaatt gcctcgatta
     1861 acaagctatt tcttaggctg cgctaatcct gaagaagtaa aatttttagt cacagacatg
     1921 aacgccgcct acttccagct taccaaacgt attctgccaa atgcgaaagt agtgattgat
     1981 cggtttcata ttatcaaaca catgaatcaa gcctttaatg agttgcgtat ccgtgaaatg
     2041 aatgaacttc gtaaagtagg acagaaaagt caggcagaaa aactgaaaaa gaactggcgc
     2101 tttttgctaa aaaatcgtgc aaacatcaac cattatgaat acaaaacatg gaaaagtttt
     2161 cgagcaccaa aatacccatt tcttacggaa gcgatgatga ttgatcgatt gcttggcttt
     2221 tctacgtccc tgaaggaggc gtatc
//
LOCUS       DAXWLH010000254         2219 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXWLH010000254 DAXWLH010000000
VERSION     DAXWLH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.260"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            199..951
                     /locus_tag="ACGE36_002711"
     CDS             199..951
                     /locus_tag="ACGE36_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8385054.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1091..2014)
                     /locus_tag="ACGE36_002712"
     CDS             complement(1091..2014)
                     /locus_tag="ACGE36_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8385055.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2029..>2219)
                     /locus_tag="ACGE36_002713"
     CDS             complement(2029..>2219)
                     /locus_tag="ACGE36_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385056.1"
                     /translation="LYSFQGLNRNIVKEIPMNEIENLTKDIYWGGQRISFSHEGKMYQ
                     FFECGPAVVDYLQENLFV"
BASE COUNT          741 a          436 c          378 g          664 t
ORIGIN      
        1 gatttagatc caatcttaag ttatgttacc atctttccta gcattaagga atcggttaca
       61 attgaacctt taaagacaaa aggatttacc ttttatcact taatagtata acataatttc
      121 gtgagaaaaa aatacaattt tttcttgacc atctcgttac gtaaaggatt atgcttacat
      181 ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa tgtcagagat atcaggagtc
      241 agtccgagaa ccttgcgctt ttatgatgag attggactgc taaaaccagc taggatcaat
      301 tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt
      361 tatcgaacaa tggcattcaa gttggatgat atccaagagg tcttagacaa tccatctttt
      421 gaccaccaga aagctttaat caagcatcag caaatgttgc tggaaaaacg ggcacaaatc
      481 gataccctct tgacaactgt ccaacagaca cttgatatgt acgaaggagg aagaaaaatg
      541 agtgacaaag aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc
      601 tatggacaag agattcgaca aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa
      661 tggatgaata tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta
      721 tttgatctca ctctttactt aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc
      781 caagctcata agaagtggtt ctcttacagc tggcctgaat atcagccaaa agcgcataaa
      841 ggactaggaa tgatgtacga agccgatgaa cggttcactg catattatga cgagcgaagt
      901 ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta atcttttaca
      961 tgatccaaca aaaaagtaca cgtaaaatga cctaataaca aaacagcaga cacccaagtt
     1021 agatttttat gctaactttt ggtgtctgct ccattctttc acaagttttt tcatcctact
     1081 ttaaatatat tcatcgttga ataaacgtat aaggtatttt cactttatcc tttttttctg
     1141 taagaaacaa ttgaaatgcc atttctccgc aagcttccag atggtggtcg atggttggga
     1201 aaccaagaag ctcgctgatc agcaaattat cgcgtccaat caccactggt gtcttatctt
     1261 gcagatagct ttgcaagatg cctgctgcaa ccatatccga atttgtaaag acaccatcaa
     1321 ctccaagctc tttaaaaaag aagcctgctt gttttccttt ctctgcatct atacaatccc
     1381 aaaaaacaag tgattcatca aaagatggaa aaagttgatg acatagctgc aaagtgattt
     1441 ttgagttata acttagcctg ccacttcgcc ccaacgtgat gcccaattgc tttatacctt
     1501 gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc tcgatcaata aagacacagc
     1561 ttgcctgttt tcccttgatt tcttcacaaa aaactagagg tccatatttt tgatatgcca
     1621 ataaatcatc tagtgtattg gcacgagacg taatgatcaa tccatcgaaa gctttagctg
     1681 caaattcttc aagatacctt cgctcttttt ctttatgata gcccgtcggt aaaagtgtca
     1741 ctttgtaatc ataatcaaac gctgcttcaa tgatcccact gatcaactga tcaaaatacg
     1801 gatgattggt ataaggcaga atcactccaa tgcttttcgt ttttccataa cttaactgtc
     1861 tggcatttgc attagggata taatcgactt ctttcatgac cgcaagtatt tctttccttt
     1921 tttcctcatc tacatatgga tgtccattga ttacacgtga aatagtagaa actgaataac
     1981 ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc ttcgccacct atacaaaaag
     2041 attttcttgt aaatagtcta ccacagctgg accgcattca aaaaactgat acatttttcc
     2101 ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata tcttttgtca aattttcgat
     2161 ttcattcatt ggtatttctt ttactatatt tctattcaac ccttgaaaac tgtacaacg
//
LOCUS       DAXWLH010000255         2180 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXWLH010000255 DAXWLH010000000
VERSION     DAXWLH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.261"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(127..1152)
                     /locus_tag="ACGE36_002714"
     CDS             complement(127..1152)
                     /locus_tag="ACGE36_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8385057.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            1511..2014
                     /locus_tag="ACGE36_002715"
     CDS             1511..2014
                     /locus_tag="ACGE36_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8385058.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT          739 a          419 c          350 g          672 t
ORIGIN      
        1 aaagttcctt tccacatttt tgtgtgaatt tgacttaatg ctgagaatct ggcttttgaa
       61 caccttgaat tagaggttgt gacaaaaatc ttgtcataac ctcttttggc acatatattt
      121 gtccttctat tctaattttt gacgaacaac cgttaaataa tgttgcaaac caaccgtcat
      181 tacccacgca acacctaaac cgatgaaact tcctatgacg atccctaaaa aacgatattc
      241 gatcaaagaa atcaagtatg ctccacgaat aagtgtagat agcatcaacg ccatcggatt
      301 aagaaaaata ttagcaactg cataatttct agcaactaga aattgtgcga ttccatatag
      361 aaaagtgatc agaaaaatgg tctgcaactg cgtaaacggc acaaatgaca agaatgccga
      421 cacacataac ccacccatcg atccaatcag atattggact tgtcgatgct tcatcgcatc
      481 caagttttct gctagcaaaa tcgatgcaca tgacaaggtc aaccaataag gattatgcag
      541 attcaagccg tgactgacat aaacggaaag aaataaggca gcgctgtaga aaacagaatc
      601 aatgatcact aaaggctctt cgtgaaatct ttcttttaaa gttgcttttt caattgtctg
      661 ctcaggccgt gaatccagta attttgtaat cactgccgca atcagggcaa aaacgacacc
      721 cataaaaaaa taaagtgaga cgatcggtaa ttgagcgatt ggcagctgca tgctcgttcc
      781 catggcagaa agcatcgcaa aaaacaagcc acctggttta gaaatatgga ataatcgcaa
      841 cacgaatctg ctggaaaaag caaccaaagc tacggcaaat ggttcaagcc ataaaacata
      901 agtcgataag atccccaaaa cgaaagcaac caacagtccg caaccgatca gaaacatacg
      961 tttcagcaaa tatttcatcg gtatgttttg ataatagaaa aaagtaaaga tccctaatga
     1021 tgaaaaactt gaaatcaaaa aatcatgatt tatgtaacct aaccataata caataaagat
     1081 aagtagcatc agattcatga tacgaaaagg attatcttgt attttattga aacgaaaata
     1141 agtcttgaac aatctatccc tccttcctag taattggaaa aaaagctgaa ccaagatgaa
     1201 atgccaatcc cgtttgtcag cattacctag tataacaact atttcttgat ttttcataaa
     1261 cttagtatca aaaagtaaac aacttggaga aattagaaca aaaaacgaac attccgagtc
     1321 gttttgacag aaaaaagata gcaacttttc caagcaactc gctatcttag ctttgttaaa
     1381 gaaaccggat tcccaacaaa aaaaagcttc taattttcgt acgataacta cgttggtcct
     1441 taaacttcat acttttttat gatttttcag cggttttcga tggtatactt tttaaatgga
     1501 ggagatgtct atgcggtatg tatcaaaatt tttattaggg atttatttat tctttctatt
     1561 atggttgatt ttattcaagc tatcatttaa tcttccacaa tttctcactt attcttacag
     1621 caatgtcaat ctcgtccctt tttctacttt ttcttttgaa aatacgacgg tattacgtga
     1681 gaccacttat aacttgattg tcttttttcc ttttggggtg cttttgaatg tcaatttcaa
     1741 gagactttct ttttctaaaa aattagggat tattttcctt gtaagcttct tggcagaatt
     1801 gattcaattc ctattcggta ttggcgtagc ggacatcact gatttgataa ctaatacaac
     1861 aggaggactg ataggtttat gggcctatca acttctaaac aaacatctat cgacaaataa
     1921 gttagaccgt ctagccatta ttttgggata cattttattc ttatttctga tcgtagcttt
     1981 tttatttttg acccgaactg tccgtcttca ataaaatcgt tcgtaaaaaa agaccgtaat
     2041 agacacattc caatgtgtat ctatgacggt ctttttattc agtcattggt ttaaaaaaat
     2101 ctattttcat ctctaatcta ctatattatt gcagcaggac gacttgcagt ttgaaactga
     2161 caggctatat tccccttatt
//
LOCUS       DAXWLH010000256         2165 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXWLH010000256 DAXWLH010000000
VERSION     DAXWLH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.262"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            76..828
                     /locus_tag="ACGE36_002716"
     CDS             76..828
                     /locus_tag="ACGE36_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFF8385059.1"
                     /translation="MKKNILGSVIKDARKKKKLTQEQLSKLTGYSQNTISNHENGNRS
                     LDEDNIKTYATALDLTPDDLFEALDIKNSLENKMMIIDKKQTKIDNLLDLYSQLEESR
                     QTKVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFER
                     VPNNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDY
                     ESQTIILHSINELYEDRVVSPEQIRIIGKVVF"
     gene            905..1114
                     /locus_tag="ACGE36_002717"
     CDS             905..1114
                     /locus_tag="ACGE36_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFF8385060.1"
                     /translation="MPNYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESLN"
     gene            1221..1874
                     /locus_tag="ACGE36_002718"
     CDS             1221..1874
                     /locus_tag="ACGE36_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385061.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSLTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
BASE COUNT          830 a          365 c          399 g          571 t
ORIGIN      
        1 atcacttttt gagatatttt tatttacacg tttatcacaa tgtgatattg ttatctcatg
       61 aaggaggtgc ctttaatgaa gaaaaatatt ttaggttcgg ttataaagga cgcaagaaaa
      121 aagaaaaaac ttactcaaga acaacttagt aaattaactg gctacagtca aaatactata
      181 tctaaccatg aaaacggaaa ccgatcccta gatgaagata atatcaaaac ttatgcaacg
      241 gctttggatt taactccgga cgatcttttt gaggcgttag atattaaaaa ttctctagaa
      301 aacaaaatga tgattatcga taaaaaacaa actaaaatag ataatttatt agatttgtat
      361 agccaattgg aagagtctcg ccaaacaaaa gtctacaact ttgctgaaca gcaactcgaa
      421 gagcaaaatc gaggaaacgt ggtgcacttt cctaaaaagg aaaaacttcc gacaatcaaa
      481 aattcagctt ctgccgccaa cccgactgaa ttagtttatg gcgatacagt tgtagaggaa
      541 gaagaatttg aaagagttcc aaacaatgct gattttgctg ttcctattat aggcgattca
      601 atggaaccag ttatcaggaa tggacaattt gtatttgtta aggaacagcc agacgtggaa
      661 gacggtgaga ttgctatcgt tgaaatagat ggtgacggcg tgacgtgtaa agaagtatat
      721 aaggattatg agagtcaaac gattattcta cactctataa acgaattata tgaagataga
      781 gtcgtctcac cggaacagat tagaataatc gggaaagtcg tattctaaaa aaatacccca
      841 gtcgaagttg gcgctccggc taggggttag tatttattat tatagtagaa agaaggaaaa
      901 gaatatgcca aattatgttg tattgcaagt tgtattaaaa gaaaagttta taggaaaagg
      961 gtcgcaaaac ctatcagaac ttgaaaacac tataaataga cagtgttcta aaggttatcg
     1021 attacacact atttccacta caaacggtgg tagtaaaggt ttcggtggtg gcgatagaat
     1081 ccaagctact ttagttttcg aaagtctaaa ttaacaaaaa acacgccctc tcccctacca
     1141 agtttgagtg acgtgagata ttagacctat atagtaggct tctttacaac ttattgtatc
     1201 aaagaaaaga ggagtataaa atgaaaaaag ttagcgttat gttgttgtta agtactgctc
     1261 tgctactttc agcttgttca aataataaaa aagctgaatc aacagatgcc acttctaacc
     1321 aagaaacaaa aataagtaaa acaaaagaaa caactgaaac cagttcatct actagcaaat
     1381 ctacatctaa aacagattct agtttaacag ttacaagctc caaccaagtt acggcggagc
     1441 ctagcccaac agttataagc tccagtcaga gtacaatcca aaccgcacct caagaagaaa
     1501 catatgaaca gatgaaacaa cgcactttac agtcaactcc agctgatcgt gcaaattggt
     1561 ccaacaaaga gtgggaagct ttcggcgtgg ccctttatga aaatggattg actacagatg
     1621 atgctggcaa tattatcagt caagatcaga aagaacaaca agcagcatct caacaaaatc
     1681 cagaagacca acaaacaagc gctcagcaag acgctgacac tttatcactt actgattttg
     1741 ttaacaaata cgggatgtcg cctgttgcat ggaaagtaca gaatggaatg tctgaagaag
     1801 aagcattgcg tacaacacag caaaagactt ccggtgaagt tcaattagga ttttctaaat
     1861 acggaattca ataatatatt tttatgccct actattttgc ctataatctc taaaaaagtt
     1921 ataaagaaaa aagcccgtgc tgcaacacgg actaaaatct cgtttctaag atcctacata
     1981 taaataatat cataggaatg aggagtaaaa aagttattag agaaagaaaa tataaaaaga
     2041 aacaaaggaa agagtaaaat aatgaaaaag aaaaaaggta cacaattatc cgttgattca
     2101 ccaaactggt tattaattat tacagtttta tgttttgcag cttttataat ggtatcaccc
     2161 caagt
//
LOCUS       DAXWLH010000257         2153 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXWLH010000257 DAXWLH010000000
VERSION     DAXWLH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.263"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..359)
                     /locus_tag="ACGE36_002719"
     CDS             complement(<1..359)
                     /locus_tag="ACGE36_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385062.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIK"
     gene            complement(372..1226)
                     /locus_tag="ACGE36_002720"
     CDS             complement(372..1226)
                     /locus_tag="ACGE36_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8385063.1"
                     /translation="MMKSKFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            complement(1306..1512)
                     /locus_tag="ACGE36_002721"
     CDS             complement(1306..1512)
                     /locus_tag="ACGE36_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385064.1"
                     /translation="MIENRVLGYVECKDNLDDTLEVLESIIFNTKVLDLKAINGDMTT
                     HIILDEISAKEIGETLIAWANKEL"
     gene            complement(1895..2110)
                     /locus_tag="ACGE36_002722"
     CDS             complement(1895..2110)
                     /locus_tag="ACGE36_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385065.1"
                     /translation="MARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFAKKF
                     IEMSEDEDIELLEKWLEDRKNNGQNDE"
BASE COUNT          671 a          433 c          235 g          814 t
ORIGIN      
        1 aattttataa ctttcaaaac tttaaaggct tcttcagaaa tttttactgg tctcgttttc
       61 aacttgccaa aatcagtaac tatactattt ccgtgtgttt caaattcttt taaaaataaa
      121 gctaaaattt ctgtcattaa tgaatattta gcaccgtatg ataaatcttt aacaacttca
      181 tcgtataact caactggtac cctaacagat ttccgtttat caccagtaga cttaatgcca
      241 gctttacctt ttaacatttg ttgtcctata ttaaatccac cactaccgct ttttattccg
      301 aacggattac ccgaaccatt actgccggca ctagttactt ttttagtttt cgacatcatg
      361 caacaccttt cctaaacacg ttctaaaata ttttctcctg tagctttttc taaacgtact
      421 aacgtttctt ctacaatgtc tacatacatg ctataagcag ttttagaatg tgggatagat
      481 ttatttagtc cttctctacc ccaacgttta attacgcctc gttctgaaac aatatgagaa
      541 taaactaagt ctccatattc ttcttgaagt tctgataaaa gttctaatcc tgtttttaat
      601 ttagtatcca ctagatatgg aacaatacct aaaatttcta aatctccgtt tacttctttt
      661 acagcagttt gataattagt aaccgtactg tgcaagttgt ctatcgaatc ttgctcagtt
      721 tgcgtgggaa ttactaaaaa atcactcgcc acgtaaacgt tatctgtaaa ttctgaaact
      781 gtaggaatag tgtcaaacaa aataaaatca tattgatctc ttaaattatc attaccgatc
      841 atataattaa acaaattcca aaagacttct tcaccttcat cagtaaaacc agtacttctc
      901 cacatatctc ttaatacttt ggtaaacctt gacatttgtt ctgaaccaag cacaatatct
      961 aagttttctt taacaggaga aatgcagtct aagaaagaca tattaattac tgcttcaaac
     1021 aatgtcattt tcccaacaac ttttaaatca tattgtcttt gaaacttttt agttactgtt
     1081 ccttgtggat caacatcaat aactaaaact ttaaagcctc tagccgatag aatatcagcc
     1141 attagctgag taagagttga cttaccaact ccccctttaa acatgttgat tgcaattaca
     1201 tatcccaaaa actttgattt catcatgtat acctcacttt tgataattta ttatactata
     1261 cagactactt gtagtcaatt ggccacttta ttttaaaatt atcttttaca gctctttatt
     1321 agcccatgct attaaagttt cgccaatctc ttttgcgcta atttcatcta atatgatatg
     1381 tgttgtcata tcaccattaa ttgctttcaa atccagaact ttggtgttaa atataatact
     1441 ttcaaggact tctaaagtat cgtctaaatt atctttacac tctacgtacc ccagtactct
     1501 attctctatc aactttagct cctccttata atcagataac aaaaaaattc tctaaaagtc
     1561 ctacaaaaaa aatctagtaa aaaaggtcaa aagtgaacga ttcattaata taattcgtta
     1621 ttttccatta tgagcggttt tgtaataatt tcaatatttt tttctacgct tgaacaatac
     1681 atagatatta agaaaatatt tagtgaaatt tatcaacgct catttaataa atcgttcctc
     1741 aaaatattca aattcctcct ttctttaggc cataatcgtc tgtttttatt cccgatccct
     1801 caattgacta cttgtagtca ttagactttt agaaaaggga tatttctatc cctaaaacca
     1861 ttctacacca ttgactactt gtagtcaatg gttattattc atcattttga ccgttatttt
     1921 tccgatcttc taaccatttt tccaacaatt caatatcttc atcttcagac atttcaatga
     1981 attttttagc aaatgatttg gctcttatat aggcaatagt ttcctgattt ttttcaaccc
     2041 attttttcct agcctttttc attgcttctt ttctttcttc ttctgtatta tattttctcg
     2101 gtctcgccat agaaatcatc ctttaagttt tatcttctac agtataataa tat
//
LOCUS       DAXWLH010000258         2145 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXWLH010000258 DAXWLH010000000
VERSION     DAXWLH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.265"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            20..304
                     /locus_tag="ACGE36_002723"
     CDS             20..304
                     /locus_tag="ACGE36_002723"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8385066.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            306..821
                     /locus_tag="ACGE36_002724"
     CDS             306..821
                     /locus_tag="ACGE36_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFF8385067.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            837..1499
                     /gene="rpe"
                     /locus_tag="ACGE36_002725"
     CDS             837..1499
                     /gene="rpe"
                     /locus_tag="ACGE36_002725"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8385068.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            1506..2078
                     /gene="hxlB"
                     /locus_tag="ACGE36_002726"
     CDS             1506..2078
                     /gene="hxlB"
                     /locus_tag="ACGE36_002726"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFF8385069.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
BASE COUNT          770 a          319 c          415 g          641 t
ORIGIN      
        1 tacgaaagaa gagatcaata tgaaaaaatt attagttatt tgtggagcag gtcatgctac
       61 ttctacaatt gcagtagcaa aattatcaaa atggttggaa gaagaaggat taacagatca
      121 agtgaaaatt tatcaatcta aaattgctga tgagctaaat aaaattgatg aatatgatgc
      181 tgtagtaagt acaacaattg tccctgatgt aattaaagct aaagtaacga atggcttggg
      241 cttattaaca ggtattggta cagatgccat ttatgcagat gtaaaagaaa gattaggtgt
      301 ttaacatgcg ttttcatcat gacactttga ctattgaaac agtttcaggg cgtccttcgt
      361 atcatgatat tcgaaaagaa cttttagata cagttgatac agcaggtatt gagaatggaa
      421 tgttagtaat ttcgagcccg catacaactt gctcactctt ttttgaagaa acgatgcatg
      481 acacaaactt ttttggtgat gattatttac atgtagacat caacaatgta atggaaacga
      541 ttattccaaa aatgacgagt gaaaatcaat acaacagtcc ggggccaaaa cacattgatt
      601 ttggaacaag attatctgat cccaattacc ctgcagaaaa atgggtcatg ctaaataccg
      661 atgcacattt acgttcatct atttatggaa ccaactcaat gactttaatt gttcaagaag
      721 gtaaactttt attaggagaa atgggccgtg tctattttgt cgattgggat caactaagag
      781 agcgaactag aacaatcaat ctcatgatta tgggagaata aacgggggaa tagagaatga
      841 caattgctcc atcattgtta aatgcaccta tcgcagaagt taaagagtta tttgaaatct
      901 taaaagaaaa gaatgttgat tatttgcata ttgatgtaat ggatggctgt tttgtaccag
      961 atatggcttt tggaccaagt gttattaaag atattcgtcc actaacagac gttgtgtttg
     1021 atgttcattt gatgattgat acaccagaaa aaattgtcac aagatttgta gaagcaggag
     1081 cagatattgt taccttccac attgaagcta ccaaacacgc aatgaaggtc attcagttag
     1141 tcaaggaagc aggagcaaaa gtagggatta cttttaatcc tgcaacacca gtcagtttta
     1201 ttgaaccagt attatcttta gtcgatcaag tattagtaat gacaattaat ccaggaacag
     1261 agaataagca ttttattcaa gagacagtag ttaaaatcga acagttagat gtaatacgca
     1321 aacaaaatga ttatacgtat gatattgaag tagatggcaa gattgataat caaacgatta
     1381 aagtttgttc taaagcaggg gcggatattt ttgtatcagg cggtttcatc ttcaatggga
     1441 aaaatacccc gcaagaacaa attgataaac taaatcaatc attagaggaa gtgaagtaaa
     1501 agatcatgac caataaagac caaaataaaa actatttaga agcgattatc ttagaattac
     1561 aaaataactc aaaacgtatt aattctgtag agttagaaca atttagtgag aaaattgttc
     1621 aagctaaaca catctttcta gctggtgcag gacgctctgg aattgccatg caagcattta
     1681 cgaatcgctt aatgcattta ggtttttcag tcagccttgt cggtgaaatt agttcaccac
     1741 attcgcaacc tggagattta ttaattatct gttctggctc aggtgaaaca ggtagtttaa
     1801 aaaatttagc ggccaaagca aatcaaagtg gtgtaagcat tgctttagtt accatgaaaa
     1861 aagactcgtc gattggtcaa ttagcagatt ctgttcttgt attaccaggt attaccaaag
     1921 atgaaaatca acgagaagaa gatacttttt cacaacctat gggttcaagt tttgaacaat
     1981 tagcatttct cacctttgat agtttagtgt tatctttaat ggataaaaca ggtgaaagct
     2041 cagaaacgat gtttgctcga catgcagatt ttgagtagta gattttaaag atattatttg
     2101 tcaaattaag cgagacaaaa ctttccattt acgtaactca aaaaa
//
LOCUS       DAXWLH010000259         2125 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXWLH010000259 DAXWLH010000000
VERSION     DAXWLH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.266"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            24..>2125
                     /gene="acm"
                     /locus_tag="ACGE36_002727"
     CDS             24..>2125
                     /gene="acm"
                     /locus_tag="ACGE36_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFF8385070.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAV"
BASE COUNT          841 a          342 c          413 g          529 t
ORIGIN      
        1 ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta tcaatgttat
       61 ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag
      121 atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa
      181 acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc
      241 tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc
      301 cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg
      361 ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga
      421 tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga
      481 gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg
      541 ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt
      601 tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc
      661 aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac
      721 agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc cccagcgcta
      781 catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca
      841 atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt
      901 ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa
      961 gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag
     1021 gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt
     1081 tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt
     1141 cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag
     1201 aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct
     1261 ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta
     1321 cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt
     1381 ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac
     1441 gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg
     1501 gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt
     1561 caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt
     1621 cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga
     1681 cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa
     1741 aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca
     1801 tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca
     1861 tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac
     1921 cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa
     1981 aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta
     2041 agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta
     2101 gtaaaaaatc tagttttgct gtaat
//
LOCUS       DAXWLH010000260         2124 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXWLH010000260 DAXWLH010000000
VERSION     DAXWLH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.267"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            580..912
                     /gene="traE"
                     /locus_tag="ACGE36_002728"
     CDS             580..912
                     /gene="traE"
                     /locus_tag="ACGE36_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFF8385071.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1531..1872
                     /locus_tag="ACGE36_002729"
     CDS             1531..1872
                     /locus_tag="ACGE36_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFF8385072.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          733 a          286 c          447 g          658 t
ORIGIN      
        1 atacgcgaag agagataatt tcattattta aacaagctaa aaatcttgat attacttctt
       61 ttcaggcaat acgaatatta ttcgcttaca ttttttgtat aaatttaatt gtctgctatg
      121 cttgtgatga attgattttc gttttgcact tttggatgat aaatttggac aattgaaaat
      181 acaaaaaaaa gaaaggcggt atgaactcgg aaggctggta accttcgtca gcgttcatac
      241 acggcattaa gatttggccc ttaatgcgtt aacctttcag tgttacggta ttacccgcaa
      301 tggatttagt ataacagata ttcaaaagtg ataccacttt ttttggctgt gttaaggtta
      361 aaaatacgtg atattacgat gaatctatgc ctaagcataa tgttataaaa atgtccgtgt
      421 ggctctttgc cgtatgacga acgctgaaat tagagggaac gcatgtttgg atgcgttccc
      481 tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa
      541 cttgttaaaa atatttaaag aaattgggtg gtttatttta tgtcatatac aggaattttg
      601 agtctcaaag atatttgtca ctacggtaaa cggtgtaccg ctacagagaa aatcacgaag
      661 aaactatcaa ctggccaaag taaaaccgtt gtgcagtgta agaaatatat aatccagaaa
      721 gacaaagtct cagaggaaat gatttattac attgggaaac aaaagcaaat aattttaaaa
      781 gatcctatcc ccttaaaaga gctatatcca acaatcaaac atgtttatga ccaaaacggt
      841 gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt
      901 ttgatcggct gaattgatca ttctcataac ttgtatttta tcgtttttcg cttgcttgaa
      961 aatattgtcg agacttctaa aggaacttag agtcactaga aaaataagga gtagacttga
     1021 tctatcaaca attcccttcg gggagtgacg gggtaggttt taacgattgg taacgtaaaa
     1081 aattggtcaa tcgagtaaat gcgcttgttt ggatagtggt gaaatgaatt tgtgtaaata
     1141 ataagcgcag agtaaacaag agggaattta gaagttagac aagttagaat agcgtctaga
     1201 ttaatcatga gcagctacta agggatctag actttgacgc aaactcatgg aataaggagt
     1261 aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta
     1321 gatacttgcg aggacgttga ttattggatc aatcgagcat ttaccgctgt ctactagggt
     1381 ttctcgtttc ggacgccata aacgagtcga aattttttat gtatttttaa gtaaacgaaa
     1441 aacgataaaa tatgaacaag tcctgaataa agtgaaaaaa ttaatataaa gcatagagaa
     1501 aatgaaattt acgtaatgga ggaatgacaa atgaaaaatt atgatccgaa tatacgattg
     1561 ggcacacata ctattaaggt ttctttccaa cgttgggatt acaaaggatt tgtgacgttt
     1621 agaaggggcg gaaattgtaa aggattagat gttttagcat tagacgagga tgatttatac
     1681 gatcaaacat tgactgataa tccaattgga ttcggcttgc tacctgaaga tgatgaaggt
     1741 aatgaatggt ttaaaatgac gcttatgaat gataatggag acgaattatc tgttgaagat
     1801 acttggtcat atttgagtga ttatattgtc agctttgaaa tcattgaatt tgtagcggat
     1861 aaagaagaat aaaaactgga ggaataaatt ttacagataa aggcttagaa ggagtcacga
     1921 cgattaataa aaaggtagta ttaccttagg cattgagtaa ggaatttatt tagtctaatg
     1981 gtgataagtc aagatgaaat ttaagatttt ctttattgag taatatgttt tgaagcgcac
     2041 gaaactagac ccgatcaaaa tcaaattttt ttcgggttga ttcaaggatt tgcgtcaata
     2101 acggattgtt atttctctat caca
//
LOCUS       DAXWLH010000261         2110 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXWLH010000261 DAXWLH010000000
VERSION     DAXWLH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.268"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            121..2109
                     /gene="treP"
                     /locus_tag="ACGE36_002730"
     CDS             121..2109
                     /gene="treP"
                     /locus_tag="ACGE36_002730"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFF8385073.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
BASE COUNT          613 a          409 c          461 g          627 t
ORIGIN      
        1 aaaacgatat cattttctct cgtacttttt aataaaacgc ttgcaactct ctccgaaaaa
       61 aagtatagta aatgaagaaa caacttgcat agacaagtta agagaaaggg gtttattgat
      121 atggcaaaat atcaagcaga tgccgagaag ctgttacatg atatcggcgg aaaagaaaat
      181 attgctgcag ttagtcattg tgcgactcgt atgcgttttg tcctgaatga tccaggaaaa
      241 gcggacgaaa aagcgatcga agatatacct agcgtaaaag ggatgttcac gaatgcagga
      301 cagttccaag tcatcatagg aaacgatgtt tctacttttt ataacgactt cgttgcagta
      361 tctggagttg aaggggtttc caaagaacaa ggaaaagcag ctgcaaaaca aaaccttcat
      421 cccgttcaac gtgctattgc cgttttagca gaaatcttta caccactgat tcctgcaatc
      481 attgtcggtg gattgatcct tggattcaga aatgtacttg aaggtatcca gtttgaaagt
      541 cttggtggaa caatcgttga acactcgaag ttttggaatg gtgtcaatgc ctttttgtgg
      601 ttaccgggtg aagcaatttt ccattttctt ccagtaggta tcacttggag tattgcaaaa
      661 aagatgggta ctacacaaat attaggaatc gttttaggaa ttacgttagt ttctccgcaa
      721 ttattaaatg cttatagtgt agcttccaca gccgcagcag atattccatt ttgggatttt
      781 ggttttgctc aagtccagat gattggttat caagcgcaag ttattccagc catgctggca
      841 gggtttatgt tagtgtatct agaaatcttc ttccgcaaat atatccctca gtcgatctcg
      901 atgatttttg ttccattgtt ttcattgtta ccaactgttt tagctgcaca cgttatttta
      961 ggtcctatcg gctggacagt cggaagctgg atctcaaata tcgtaaatac tggattgacg
     1021 tcttctatca gctggttgtt tagtgccgta ttcggattcc tgtatgctcc tttagtgatt
     1081 actggtctcc accatatgac caatgccatc gatatgcagc tgattgcaga tttcggatca
     1141 acgaatttat ggccgatgat tgctttatca aatatcgccc aaggctctgc agtcttagcc
     1201 atcatcttct tacatcgtgg gaataaaaaa gaagaacaaa tctcgattcc agcaatgatt
     1261 tcttgttact taggcgtaac tgaacctgct atgttcggaa tcaatttgaa atacgtctat
     1321 cctttcgtcg ctgcaatggt cggctctgga ttagctggca tgtttgctaa cttgatgggt
     1381 gttcgagcta acgcgatcgg agtaggcggt ttgccaggaa tactagctat ccaagcagaa
     1441 acatgggtac ctttcatcat cgcaatgatc attgctgtca tcattccatt tggcttgacg
     1501 atcatcttta gacgtcaggg aattttgaat aagattgatc ctgctgttcc tgaaaatgct
     1561 gctgatgtac aacttcaaac agcaaatggc gcaacagcaa cgccacagtc tttcgaacct
     1621 gtttcagcaa ctggtacggc agttgcaaca aaagaaacat tattcgcagt cgctgcaggg
     1681 accatcaagg aaataactga agtaaatgac ccagttttct cacaaaaaat gatgggtgac
     1741 ggttatgctg tcgaaccgtc aaacggcaaa gtctatgcac cagtaaacgg taaagtgacg
     1801 agtgtctttg aaacaaaaca tgccattggt attctttcta atgaggggct ggaagtactt
     1861 gttcacatgg gattagatac tgttgaacta aagggtgttc ctttcaatgt attcgtgaaa
     1921 gaaggatacc ttgtcacacc agagaccctg attgctgaaa tggatttgcc agaaatagaa
     1981 caagctggca agaaaactga cattattgtt gctttgacca ataatgaaaa agttgcagga
     2041 ctttctcttg accaatcagg acttgttcgc ccgggcgaag cagtcggtaa agctgaagta
     2101 aaatcttagt
//
LOCUS       DAXWLH010000262         2087 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXWLH010000262 DAXWLH010000000
VERSION     DAXWLH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.269"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            84..407
                     /locus_tag="ACGE36_002731"
     CDS             84..407
                     /locus_tag="ACGE36_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFF8385074.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            589..1959
                     /locus_tag="ACGE36_002732"
     CDS             589..1959
                     /locus_tag="ACGE36_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFF8385075.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
BASE COUNT          587 a          385 c          427 g          688 t
ORIGIN      
        1 cctcgttgaa gtaagtattt ctagtttatc ctaggattaa ttataaaaat aaaacattcg
       61 ttgattcgaa ggaggttaaa cagatgtttg aaagttttga tagcagccgc aaccgttacg
      121 cttcagttgg tgtcgtatca agtttgccag atgaattgat tgacagtata tggtttatta
      181 ttgacctaga tcttaaggga gtcattccat tagaaaatat attaaccttt gatttgatcg
      241 aaaatcgtgg aaaagtgacg atgcattttt ctcaagcagg aagcaatgtg gaaatggata
      301 ttgatttgcc gtttgcctat tcgaaccaat ttcctgctca agtatacgcc tatgatgacg
      361 gtacgagaga aacgatcctt ttaccaagtg agattagaca atattaaggt gatagataaa
      421 cgtggatgtc ccttatttta cagaccaggc caagttgttg attctttgac tcaattcttg
      481 gcttcttttt atggagtttc ataaattatc attgatttct aattggtatt ctcaggataa
      541 acgctatata ctactaacta aattttaaag atataaggag tttaaaagat gcgcgattta
      601 acaaaaggaa gccccgctaa actgattttg atgttcactg ttcctttact gatcggaaat
      661 gtttttcagc agttctacaa tatgatcgat atgatcatcg tgggtcagac gctaggtaaa
      721 aatgcgctgg cagcggttgg tgcaactgga agcctgacat ttttgatcat tggctttgcc
      781 caaggtttaa cagctggttt agccattatt accgcacaac gttacggtgc aaaagattat
      841 cggggtctga agaaaagttt tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt
      901 ttaactgtcc taagtctcct gtttattcgt ccgatgcttc aattgatgca aactcctcct
      961 gaaatacttg atcaagcaca aacatttatt tcaattattt tattagggat ctttgcatca
     1021 gtcagtttca atttgctttc taatgtcatt cgcgcacttg gtgacagccg cacaccacta
     1081 ttctttctga tcattgcggt catcatcaac gtcgtacttg atttgatctt tattatctat
     1141 tttggcatgg gtgtggaagg tgcagctatt gctacagtaa tcgcacaagt ttcttccagc
     1201 gtactgtgtc tagtgtatat caagaaaaaa atgcctttgt tacagttacg caaaaaagat
     1261 ttcaaatttg aaaaagaaga gtttgctgta cacctaaatg ctgctttgcc aatggcgttt
     1321 caatcttcga tcatcgctgt tggtgctatc gtgttacaag cagcactaaa tagtctaggg
     1381 acagatgtcg tagctgctca agccgctgct ggtcgtatcg accaatttgc aaatcagccg
     1441 atgatgtcat ttggtattgc aatggcaact ttcactgcac aaaactatgg tgcgaaagaa
     1501 tatggccgaa tcttgcaagg ggtacgtcaa actttactga tgagtgtcgg atttagtctc
     1561 ttagcaggtg ctatcgtcat tttcttcggt catttctttg ttcaattgtt tgttagccca
     1621 tcagaaacac gtgtatttga attagcacaa acctacttca atatcaatgg gtctctttac
     1681 tggatcttag cagtcctgtt cattttacgc tacacacttc aagggcttgg acaaagcaag
     1741 atccctacga tcgccggtat gatggaattg cttatgcgtt cttttgcagc tatcgttctt
     1801 acaggaatgt ggggataccc aggagcagca gcagcttctc ctttagcttg gatcggttct
     1861 gttgccgtgt tgctttactc ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa
     1921 gaggaacatt tcacattaga acatgcagaa gcaagatagc cttgattaaa agaaatcgtt
     1981 gcggctaaaa atgaaaactt ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt
     2041 ttagataaca aaaaggctgt gacaaaaaat gtcacagcct tttttct
//
LOCUS       DAXWLH010000263         2079 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXWLH010000263 DAXWLH010000000
VERSION     DAXWLH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.270"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            20..2077
                     /locus_tag="ACGE36_002733"
     CDS             20..2077
                     /locus_tag="ACGE36_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFF8385076.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
BASE COUNT          748 a          382 c          382 g          567 t
ORIGIN      
        1 aaatttagga gttgaaaaaa tgaaattatt taatccaaaa actgttttag ctattggaac
       61 atttgctgcc tcacttagtc ttgctggatc tgttgacgct gcttcaacta acgtcatgta
      121 tcgcctttac aatccaaata ataatgaaca tttttacacc tctagcaaaa aagaacgtga
      181 ccatctagta aagataaaat ggggaaatta tgaaggcccc gcctgggaag caccgactag
      241 cggtggccat cttgtctatc gtttgtacaa taaaggtctt cgtgatcacc attacacagc
      301 tagttgggat gaagtgaaat ggttgactaa aaattacggc tggacttatg aagggcctgc
      361 ctggcgttct gccgaaaaaa ataataaacc gatctaccgc cttttccttc ctggtgtaaa
      421 atctggctca catcactaca ctgcaagttg gaaagaagta caatggttaa ccaaagacta
      481 tggctggaaa tacgaaggta tcggttggta tggcgctgac acctctaaac ccaaagtgaa
      541 taaatcggct ctagaagctc tttatacgag cgttaaggga acgaaaaaag gtgactatac
      601 cgacaatacc tggaacgcat tccaaaccgc tttaaacaat gctaagaaag tgttaaatga
      661 ctcaaaagcg actcaaaagc aagtggatag tgccaaaaat aaattagatt ctgcttacaa
      721 aggattaaag aaaaaaccag ttcctactgt gaataaagct gaattacaag ctttatacaa
      781 ccgagtaaaa tcaactgcta aaggagattt tacagaagaa tcttggaata attttcagac
      841 tgctttatcc aatgctaaaa aagtactaga cgattctaag gtttctcaaa cacaagtaaa
      901 caatgctaaa aatagtcttg atgctgcatt taaagggttg caacacaaac ctgaaacacc
      961 aaaagaatat acggttacta ttaaccatat tgacaatcaa acaggtcaag tgattgctac
     1021 agatcaagca aaagtaactt ctggttctac ttacactgct aaggcaaaag catttaaata
     1081 tagcgaaggc gctaaagata attttagcta caaagtgatt ggtaatgata ctcaaagcaa
     1141 aacaatcaca ggtaatacaa caatcacatt caattataat caagtgcatc aaatcaattt
     1201 gttgtgcaat aacaactaca aaaacgaagg acgagttaat attttgaaag aacagatagt
     1261 caatgtcact cacggtgaca gtgtcacttt aactgctcct tctattcaag gatatgtttt
     1321 agacgatcgg gtagaaccaa caaattcagt aaccttaaac aatgtgactg aaagtcaaaa
     1381 ttatattttc aactacactc gtaaatttaa tgtgactgtt aatcatgtaa atgctgatac
     1441 caacgctgtt ctctctagtg aatcaaaaac agtatacgaa ggggatagct ttgctactcc
     1501 ttggaaaaac atgactgatc aaaattattt cttgtgcaga aatgacgatc ctaccgttac
     1561 cgtggataaa aacggccaac gatccattaa taacgtcgat caaaataaaa caatcacatt
     1621 caaatataaa aagatttcat tagacgaatt acgtaacttt atgcgtcaaa aagaacttgc
     1681 ttggttaaat gattatcgcc aacaaaatgg tgttgcccca atgcaattca atgatattgt
     1741 tcaacaggct gcagatattc gagctaaaga attacaagtc agcttcagtc actatcgccc
     1801 tggtggtggt actttccaag atttattgga aagcttaggc tgctacggtg ctaagggaga
     1861 aaacataagt caaactgggc tgtatattga tgacctttta tcagacgatg gtgctatcga
     1921 tgccatggta ggatggaaaa atagccctgc ccataatgca aatttacttt ataataacca
     1981 aagtattctc ggattaggtc ataattttgt agttgactct gctggtagaa ttggcgtaga
     2041 taatgtattt attagtgcga atccaatctt caaataaaa
//
LOCUS       DAXWLH010000264         2063 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXWLH010000264 DAXWLH010000000
VERSION     DAXWLH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.271"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            221..916
                     /gene="vanR-A"
                     /locus_tag="ACGE36_002734"
     CDS             221..916
                     /gene="vanR-A"
                     /locus_tag="ACGE36_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8385077.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            894..2048
                     /locus_tag="ACGE36_002735"
     CDS             894..2048
                     /locus_tag="ACGE36_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8385078.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          688 a          390 c          441 g          544 t
ORIGIN      
        1 taattagcca ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat
       61 aaaatattaa cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat
      121 cttaagaaat tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga
      181 taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat
      241 tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta
      301 tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat
      361 tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa
      421 aataagggac aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga
      481 taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact
      541 ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa
      601 ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga
      661 gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat
      721 cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg
      781 cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga
      841 aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta
      901 taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata
      961 tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag
     1021 ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg
     1081 acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg
     1141 cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact
     1201 ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc
     1261 tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa
     1321 agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg
     1381 cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg
     1441 ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt
     1501 atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga
     1561 taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat
     1621 tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga
     1681 ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg
     1741 ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg
     1801 tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg
     1861 aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat
     1921 tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg
     1981 ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga
     2041 ggtcctaaga gatgtatata att
//
LOCUS       DAXWLH010000265         2049 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXWLH010000265 DAXWLH010000000
VERSION     DAXWLH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.272"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..248
                     /locus_tag="ACGE36_002736"
     CDS             <1..248
                     /locus_tag="ACGE36_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385079.1"
                     /translation="VTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIVSYSI
                     LTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            455..655
                     /locus_tag="ACGE36_002737"
                     /pseudo
     CDS             455..655
                     /locus_tag="ACGE36_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1367..1972)
                     /locus_tag="ACGE36_002738"
     CDS             complement(1367..1972)
                     /locus_tag="ACGE36_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385080.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          663 a          348 c          361 g          677 t
ORIGIN      
        1 aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat atttcatttc
       61 taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac tggggtattg
      121 tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt tataatgctt
      181 ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag aaaaaaacaa
      241 gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag aatgaaacac
      301 attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta agttttttca
      361 tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc ccgttattga
      421 agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa tatttgagaa
      481 acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga tcacctccta
      541 ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt gccagttaga
      601 tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga attgatcaga
      661 aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg ctcagggcaa
      721 ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc actttagaat
      781 acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat ttaaacaagt
      841 tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca aggcaataga
      901 tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga ataaccttct
      961 gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta ttttattggt
     1021 tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa cgattagttt
     1081 aactgtggga agctccagac atacgattcg agaagttact tctcgagcag acaggaaggg
     1141 ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc tttttccgga
     1201 aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc ataaagaact
     1261 aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac atggaacata
     1321 gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac aaatcattcg
     1381 ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca atttctgcaa
     1441 ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt ttttgcggat
     1501 ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct tttttggcca
     1561 ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt gcaatataac
     1621 ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt ccagtattaa
     1681 aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc ataaaggtaa
     1741 ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca acaatgacac
     1801 aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg atatctttac
     1861 cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt gccaattgtc
     1921 gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc ataaaaactc
     1981 cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact gttatcatag
     2041 catttttaa
//
LOCUS       DAXWLH010000266         2028 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXWLH010000266 DAXWLH010000000
VERSION     DAXWLH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.273"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            38..367
                     /locus_tag="ACGE36_002739"
     CDS             38..367
                     /locus_tag="ACGE36_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8385081.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            357..644
                     /locus_tag="ACGE36_002740"
     CDS             357..644
                     /locus_tag="ACGE36_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8385082.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(923..1486)
                     /locus_tag="ACGE36_002741"
     CDS             complement(923..1486)
                     /locus_tag="ACGE36_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385083.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          725 a          307 c          328 g          668 t
ORIGIN      
        1 aagtaattac gaaatgtaaa ataatggagg aaataatatg attacaaaaa caagaaaaca
       61 aggaaattct attatgttaa cggtccctaa agaattcgat gtgcccaatg gtgttgaagt
      121 agaagctaag cttgtagaga atggtatcct ttatgagttt gtagagccta aaaaagaatt
      181 ttttgatttt agtgaagata ttttggctga tattctttca gaaggttata gcaaacaaga
      241 gatcttaaaa gaatttagaa atagaaaaaa tgcgttaact tctgcattta aatctattgc
      301 tgaagatact gttgcaaatt ctaaaccgat gacaaaagag gagttggcag cagaaattgg
      361 cttatgagat tctaccttct aaacacgtaa taaagtatct aaaaaaatta aaagagaaaa
      421 cattaaaaga acaattttta acgataattt atgatgagat agcagttcgt cctcattcgg
      481 gagaacaaaa aacgggtgat ttatcaggaa tttgggctat tggattcaag tatgcaggta
      541 cgacatatcg cgttgcatat gaaattaaag ataatgcagt gatacctaac ttactatgtg
      601 gaacgcatga aaatttttat gaacaattaa aaaagattag gtaagaaccc aatgtttcaa
      661 cttgagagga tagacgacac tttaaaagat aatttttaac agctgagagt tgtaataccc
      721 tataatttta aaccactaac ctatatattt atgaaaaata gaaaaaatta aaacgcattt
      781 taagcctctt tcaaattaaa agcttcatcc ttatgcacaa agagggctta aaatgcgtta
      841 ttttatattg ttttttagta ataataaaaa aatcactcta attctcccat aaatattata
      901 gattcctaaa gattaaaata gattattccc tattgtcttt taagtagcgg tagagtgttg
      961 ccttgctaat accagtcatt tccgtaattt cttttacgct ataaacctga cttttataca
     1021 attttactgc tcgatcaaca tctttctggt tcactcgtgg acggccacct ttacgacctc
     1081 tcgcacgtgc acttttcaaa ccttctttgg tacgttcaac aattaaatca cgttcgaact
     1141 gactaaatgc ttggaagaca gtcatcatta agcgtccgtg aggcgtgctg gtatcgaagt
     1201 tttctttcaa gctcaccagc ttaacatggt gttcttcaaa ataggtaaca aggtcaatca
     1261 aatcctttgt actacgaccc aatcgagaaa aactttctac aattaccgta tctcccggac
     1321 gaattttatc ttttaaacga tttagttggg gacgaaccgc tttggttccc gtcattttct
     1381 cagttaggat ttcattacaa ttttgttcat tgagtaagtc taattgacga tttagttctt
     1441 gtgaacgtgt actcactcgg gcataaccat aaatgtatcc actcatgctt ttcttccttt
     1501 cgtactttaa ttctggcttg attgtatcat aaaagggagt tatagataca tagttaatgc
     1561 aacaagttat gatacaaaat atgacctctt ttatttaata aaagaggtca tttcattttg
     1621 tctcataatc gattgttttt gatacgagtg tgtatacgaa ataaacaaaa aaatcttaag
     1681 taaatttcta gatacctaat ttaatcctaa ctagtgtatc ctaaagttaa taacgagttt
     1741 atcacaatga aaattataaa atgctcccat attagatata acatgcaata tttgaattag
     1801 aactacaaaa tttattcgtt ttataaagta aataatgttg agatactaag gaatagaata
     1861 ttataaggaa ttgagctgaa tgtattggat aaaaaaagaa taactttatt agagatggac
     1921 tataaaaatg ctcgtaattt tcttctgaag tctgaaagct atttgaatgc tcagctacca
     1981 gaatatatta gttttgacga aactttaaaa tctgctaaaa aagtttta
//
LOCUS       DAXWLH010000267         1987 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXWLH010000267 DAXWLH010000000
VERSION     DAXWLH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.274"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            270..836
                     /gene="amaP"
                     /locus_tag="ACGE36_002742"
     CDS             270..836
                     /gene="amaP"
                     /locus_tag="ACGE36_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8385084.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            849..1037
                     /locus_tag="ACGE36_002743"
     CDS             849..1037
                     /locus_tag="ACGE36_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8385085.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1050..1607
                     /locus_tag="ACGE36_002744"
     CDS             1050..1607
                     /locus_tag="ACGE36_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8385086.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1639..1881
                     /locus_tag="ACGE36_002745"
     CDS             1639..1881
                     /locus_tag="ACGE36_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8385087.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          722 a          307 c          406 g          552 t
ORIGIN      
        1 aaatagaatc ttgtaataat aactgagcat aatctttgca atttttaatc ttgaataata
       61 tcctttaagt atagccagca atcaaaagta aatagagaat aagtaagcaa ttgttatttc
      121 agtatcagac tggcaataaa acgccgttat gatccagcaa tgtttggtag cgctttattt
      181 actgatgcta tgctaactgt tcgctatcta tactgtgatt gtcggtagag ttatttacag
      241 ggtaaaaata agtttatagg aggtggaaaa tgaataaggg gttaaaaata atcggtgtta
      301 tcatgagttt actgctactt tcagtatttc tagcgattgc gttgatcaat gcgccgtata
      361 ttatgcctga acaattggaa cgatttcgct tctttacttt aacgaattac tacatgcaac
      421 aatatatttt ctgggcagct gtagtatttg ccgttcttgc tattatcatc ttgctagtcg
      481 ttcttttcta tccaaagtca agaggaactt tcgttatgaa acgggaagat ggcaaactaa
      541 caatcgacaa aaaagcgatc gaagggttgg tacgttccca tttacacgaa gaagagttca
      601 ttcattcacc taaagtacgg atacgttcaa cgaaaaatcg aatccatatc catgtcaacg
      661 gagacttgaa acgaacttct tcattagtag ggaaaacagg tgtattgatg caagatatcg
      721 aagaagaagt aactaaagta ctcggaacaa aagaaacggt caaagtggca gtcacataca
      781 gcggttatga agaacaagaa gatacaagag actacaagca ctctcgagtg gaataggagg
      841 gccaaataat gaaagaacta ttttcagcat acaagctccc tattatcttt gggggtcttg
      901 gactactgct agctgtttta tggttaacga ttggattttt caagacattg ttgatcataa
      961 tcatgacagc aattggcgca ggaatcggtt tttatttgaa acagtcagga atagttgatc
     1021 aattttttaa atcataggag gaaacaaaga tggaaaacaa aaacatgaat acaacaaatg
     1081 gatcagtttt ggcaaatgaa aaaggagaaa accgcatgat gaatggtgaa ttgacttatg
     1141 aagacaaagt catccaaaaa atcatcggtt atgcattgaa agatgtagat ggtctattga
     1201 cattagatgg cggatttttc tcaaatatgg ctgaaaaatt agtcaacaca gataacgtaa
     1261 cagcaggtat caatacagaa gtaggtaaga aacaagtagc tgtggatcta gatgtgatcg
     1321 tagaatacgg taaagacatc gaagatattt acaaccaaat caaagaattg atcagtactg
     1381 aagtaaacaa tatgacacat ttagatgtta tcgaagtgaa tgtgaatgtt gcagacatca
     1441 aaacacaaga agaataccaa aaagatcaag aaacagtaca agacaaagta acagaagcag
     1501 caaaatccac aggacaattt gcttcacgcc aaacagataa agcaaaatct gctgtaggca
     1561 aaggtgcaca aaaagtcaaa gaaaataacg aaccacgtgt acaataagaa gtaagaagtc
     1621 agttaggagg aatttaaaat gggatttatt tgggcattaa tcgtcggcgg tatcattgga
     1681 gcaattgcag gagcaatcac gaataaagga tcatcaatgg ggatcatcgc aaacatcatt
     1741 gctggtttag taggttcagc aattggacaa gctttactag gaacttgggg accaacaatt
     1801 gcaggaatgg ctatcattcc atcattgatc ggtgcaatca ttttagtatt agtcgtttca
     1861 ttctttgcac gtaaattata agcaaattga agaaaaactt atgttgaaat gaagaacaaa
     1921 tttgagaacc gtagcgacac tcgctgcggt tcttttttat atattttata tgaagtattc
     1981 tttattc
//
LOCUS       DAXWLH010000268         1975 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXWLH010000268 DAXWLH010000000
VERSION     DAXWLH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.275"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..378
                     /locus_tag="ACGE36_002746"
     CDS             <1..378
                     /locus_tag="ACGE36_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFF8385088.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACGE36_002747"
     CDS             426..1826
                     /locus_tag="ACGE36_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8385089.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          550 a          333 c          428 g          664 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggt
//
LOCUS       DAXWLH010000269         1964 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXWLH010000269 DAXWLH010000000
VERSION     DAXWLH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.276"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            103..1653
                     /locus_tag="ACGE36_002748"
     CDS             103..1653
                     /locus_tag="ACGE36_002748"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8385090.1"
                     /translation="MSDKNTYACRSQRHKQESDTYENPDQLNETVTSKTANENKQSRI
                     PKKNSNYSKYIFALFTALILVTVATAGYIVYKLKQQEAEAKAKYETAAKNLMASIQEE
                     QDQSRISTKIDTISDGENKCLVYRPVDESAVPFKNANQLLDELAQKQQKKHCEKEVLT
                     VARIKATAISSKVGQYSIEADSFIWDRSKEHFKNPDNISEKAIYVSEKTGKEITNKDL
                     IPDEGSLLGIQQVIQQSILDHAEKPEKIIDAVLTMDRISYDSKMTYTPDELTIILPNN
                     SIGTSKVTLNYKDIAPFIDTDLVSQESIKDALPALDENKKYVALTFDDGPNNSSTLDL
                     LNILKTDNVKATFFMLGQMVDQNPDVAKQAHDEGHEVACHSYSHPQLNALSTDELRSE
                     INEANKAIFKATGVLPRNIRPPYGAIDKKSAETIGMPIIQWDIDSQDWKSKNPRTINN
                     VVKQNVYSGAIILIHDIHPESVKAIPNMISMLKNEGYEFVTIDQLLSGKQKTLHQYFG
                     MNDERLVD"
BASE COUNT          756 a          313 c          337 g          558 t
ORIGIN      
        1 aattactacg gcaaaagtaa aaaaatagtg aaaaatgcca ggtaaaaaat aagaaatttc
       61 tatgttttat ttatatgatg ataaatggag gaataaggcc atatgtctga caaaaatacc
      121 tatgcatgtc ggagccaacg tcacaaacaa gaatctgata cctatgaaaa tccagatcaa
      181 ttgaatgaaa ctgtaacgtc caaaaccgca aatgaaaaca aacaatcaag aataccgaag
      241 aaaaattcga attatagcaa gtatatattc gcattgttta ccgcacttat tctagtaact
      301 gttgctactg ctggctatat tgtctataaa ttaaaacagc aagaagcaga agctaaagca
      361 aaatatgaaa ctgctgcaaa aaatctcatg gcttcaatcc aagaagagca agaccaaagt
      421 agaatttcga cgaaaataga tactataagt gacggagaaa ataagtgcct tgtttatcgt
      481 ccagttgatg aaagtgctgt tccttttaaa aatgcaaatc aactcttaga tgagcttgct
      541 caaaagcaac aaaagaaaca ttgtgaaaaa gaagtcctta ctgttgccag aataaaagca
      601 accgcaatct cttctaaagt tggtcagtat agcattgaag cagatagttt tatctgggat
      661 cgtagtaagg aacattttaa aaatccagac aatatttctg agaaagccat ttatgtttcc
      721 gaaaaaactg gcaaagaaat tacaaataag gatttgattc cagatgaagg aagtctctta
      781 ggtattcaac aggtgattca gcaatccata cttgatcatg ccgaaaaacc tgaaaaaatt
      841 attgatgctg tattaacaat ggatagaatt tcttatgata gtaaaatgac ttatacacct
      901 gatgaattga ctatcatctt accaaacaat tcaattggaa cttctaaagt cactctaaat
      961 tataaagata ttgcgccatt cattgataca gatctggttt cgcaagaaag tataaaagat
     1021 gcattacctg cgttagatga aaataaaaag tacgtcgctc tcacttttga tgatggtcca
     1081 aataatagct caacacttga tttgttgaat atactaaaaa cagacaacgt gaaggctact
     1141 tttttcatgt taggacagat ggtggatcaa aatccagatg ttgcgaaaca agctcacgac
     1201 gaaggtcatg aggtagcatg tcattcgtat tctcatccac aattgaatgc actttcaaca
     1261 gacgaattac gaagtgaaat aaatgaagct aataaagcaa ttttcaaggc aacaggtgtc
     1321 ttgcctcgca atatccgtcc cccttatggc gctattgata aaaaaagtgc tgaaacaatt
     1381 ggtatgccaa ttattcaatg ggatattgac tcacaagatt ggaaatcaaa aaatcctaga
     1441 acaatcaata atgttgtcaa acagaatgtt tatagtggtg caatcatctt gatccacgac
     1501 atccatcctg aatcagtcaa agcgatacca aatatgatct ctatgttaaa aaacgaagga
     1561 tacgaatttg tgacgatcga tcaactattg tcaggaaaac aaaaaacatt gcatcaatat
     1621 tttggtatga atgatgagcg gctagtagac taactgttat tagattgctt ggtatgtgta
     1681 gaagttatac ttgcaattct ctctcagaaa aaatgttaag caaaacaaat tgataaagag
     1741 tatcgagaat gctaattctt attagtcaaa gttctatttt agtaaataaa aaaagtactg
     1801 gaagttttgt gatcaaaaat cacatcacaa aattggttga aaaaacattt ctttttataa
     1861 tgccactcat tattgaaacc tttttgaaaa agtttataca agctagatat aactttatta
     1921 gtttacagaa tgcaattaat gcgatgaaga tttattgagt tgat
//
LOCUS       DAXWLH010000270         1963 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXWLH010000270 DAXWLH010000000
VERSION     DAXWLH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.277"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..435
                     /locus_tag="ACGE36_002749"
     CDS             <1..435
                     /locus_tag="ACGE36_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8385091.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACGE36_002750"
     tRNA            complement(759..842)
                     /locus_tag="ACGE36_002750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACGE36_002751"
     CDS             1370..1870
                     /locus_tag="ACGE36_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8385092.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          640 a          367 c          331 g          625 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat ttt
//
LOCUS       DAXWLH010000271         1954 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXWLH010000271 DAXWLH010000000
VERSION     DAXWLH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.278"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACGE36_002752"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACGE36_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFF8385093.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          699 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaa
//
LOCUS       DAXWLH010000272         1951 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXWLH010000272 DAXWLH010000000
VERSION     DAXWLH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.279"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..359
                     /locus_tag="ACGE36_002753"
     CDS             <1..359
                     /locus_tag="ACGE36_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385094.1"
                     /translation="GSGKTERVMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTN
                     NDLKVVDDILNIYPDFNLVVFDETIPVTEIKGFYQSFSNLANKYEEVLFVWITEENSN
                     SKSAFFSEKNAFLHSI"
     gene            375..>1951
                     /locus_tag="ACGE36_002754"
     CDS             375..>1951
                     /locus_tag="ACGE36_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFF8385095.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDECH"
BASE COUNT          713 a          261 c          383 g          594 t
ORIGIN      
        1 atggaagcgg aaagacagaa agagtgatgg ctagtattga taattcaagg cctagatcaa
       61 gagcattagt aataactgat gaaaaaaatc actataaagg catacgaacg atagaaaaaa
      121 aatatttaac taacaatgat ttaaaagtag tagatgatat tttaaatatt tatcctgatt
      181 ttaatttagt tgtttttgat gaaacaattc ctgtgacaga aataaaagga ttctatcaaa
      241 gcttttccaa tctagcaaat aaatatgagg aagttctgtt tgtttggatt acagaagaaa
      301 atagtaatag caagtcagcg ttctttagtg aaaagaacgc ttttttacat tctatttaaa
      361 aaggagaatt gaacatgatt gtaagtaaaa aattagcaag aaatctagta agtgaagggt
      421 atgacttaag attagtagaa aagtatcaac cgagcggtgg aattaaattt gatgagcgtt
      481 atatttctac tggtgatgga tatgtaactt cgattgctgt tattgagttt gcagaagatg
      541 taaatagtcg atggttaacg gatataatgg caattcccaa tactattgca actttcgatg
      601 tatgtacggc ggataaagaa gacttattac acaaaatcaa tacgtctatt ggagaattag
      661 attaccgatc aacaaatgag aatagaacaa ctgacaaaac agacgcttta ctagataaat
      721 acaatttaga agattttgca caagaaatta cgaaacgtgg tgaagttgtc aaaactgttc
      781 atgctcgtat ttttgtttat agcgattctt tagaagaatt agagaaaaga attagtgaga
      841 ttcgtaaaga tttggctagt gtcaatcaca aggcaattgt tccgcaattt agaatgaagg
      901 atgaatggat gtctttattt caagatggca tggaacaaga atcaaaatta tttgccaaga
      961 aaggcttaac tgttccggca aaaaatattg gtggcggtat tccatttcac cacaagagtt
     1021 tgaaagatcc acgtgggttt tatttaggca ctacgtcaac tggtggtgct tttatatttg
     1081 atccatttta ttctacgaag caaagacggt cgtttaacgg ctttatttta ggaaagatgg
     1141 gttttggtaa gtcaacttta attaaacagc tacaggaagg cctacaagcg aagaattgtt
     1201 ttattcgagg atttgacaag tcgggtgaat ttgaaggcat ggtgaagaaa gcaggcggtg
     1261 tatacgtcga tttaggcggt ggtgatatgg tagtaaatcc attgcaaatt tatgcaacag
     1321 tcatgaaaaa agatcaattt ggaaatgatg ttgtagatga atcaaagagt tttcaaaatc
     1381 atatcactaa gtgcagtaat caaattcgct ttttagaacc tgaaattgat acggcaactg
     1441 tacgtgaata tactttgatg ctacaagact tttatattca ttatggaatt ttgccaaaag
     1501 aatggagaga agcgagagac gacaacgaac gaaatcaatt aaatacaaat atttgtaact
     1561 tgcctaatga ttcatatcca atctatggag agtttcttaa atttattgaa aatcaacaat
     1621 tcaaaattaa aaatgctact cctgaacgtg tccgttctat tgagctaatt aagcttatcg
     1681 ttacggatat gtgtacaact tatccgcaaa ttttcaacgg agtaacaaca atgccaaact
     1741 ttgatacaac aaaaattgtt tactataaca ttgattctat tagtaaaatg agtgccccag
     1801 tattccattg ccaattattt acagcgttaa cgactatttg gaacagtgcc ttgcaaaacg
     1861 gaaagattca aaaagaaaac tttaaaaaat atggcagtga ttattataag tatgcacaag
     1921 atttcatggt tttgatcgat gagtgtcaca a
//
LOCUS       DAXWLH010000273         1936 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXWLH010000273 DAXWLH010000000
VERSION     DAXWLH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.280"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..567
                     /locus_tag="ACGE36_002755"
     CDS             <1..567
                     /locus_tag="ACGE36_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385096.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            complement(614..850)
                     /locus_tag="ACGE36_002756"
     CDS             complement(614..850)
                     /locus_tag="ACGE36_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385097.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            948..1127
                     /locus_tag="ACGE36_002757"
     CDS             948..1127
                     /locus_tag="ACGE36_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385098.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1258..1527
                     /locus_tag="ACGE36_002758"
     CDS             1258..1527
                     /locus_tag="ACGE36_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8385099.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1520..1777
                     /locus_tag="ACGE36_002759"
     CDS             1520..1777
                     /locus_tag="ACGE36_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8385100.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          703 a          285 c          395 g          553 t
ORIGIN      
        1 gaagaacaat tgtatttgga aagtgaaacg agtcaaacga aggtcacagt cagccataat
       61 cgtttagacg tgtataaacg tcgtcctgaa acggtaaaaa aaggaacgga gcttctacga
      121 ctagaaacag aaaaatacat catgaatgtt tacctagaaa ggagtgagct ggtcgaaaac
      181 tttgtcagct acacgatttg caggaaacac tattactcag aatcagaata ttattcaatt
      241 tttgagagca tggaattaat ggaagaagaa atggaagagc taaacagcaa agatgcgacg
      301 atagacgatt attaccttgt atttactaga tttttgacgt gtggaacgga atctatcgtg
      361 ttaatgggaa tggattatga actcgctgta cccgaagcat ttttgaggga atatgtagaa
      421 gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt agacacctat
      481 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
      541 cgatttgaaa ggaaaaaaag gcaataatca tttataccga aaagtgagaa tgtgtgaaat
      601 aaggacaaaa aacctagtaa ctaagggaat ggttcttatt tcgcaccaat cgctccaacc
      661 gcccccgccg cttaggtggt gcgaaataag cgggcaacac ccgataacct aagagaaaga
      721 aggagatcca tatgaataaa atcattcctc tattgttagt cctatttgtt ggcgaaatac
      781 ttatgcgcca tgctgcagct gtgcatttaa acttcgtagt atttttggca atactggtat
      841 tttgtttgat cttttggcgc tttgtattag cccttgtgag tctactattc aagatgattg
      901 cagccatatt tttcataggg ttatttcgat gaaaggagag agtactaatg ccaattgcaa
      961 taggaaataa acggttaccc gttacgttgg atgaaaagag acaaaaagaa ttgcagcaac
     1021 taaagcagaa gtacggcaaa agtgaatcca ggattatgtg tattgcgtta gatttattga
     1081 ttgcccaaga aaaagcagga tttgaggtac cagcactcaa aaagtgacgt cactttttat
     1141 cctaaaaact aaaagtgata gcacttttaa ttataagaag ttagaatatt aatcatttgc
     1201 ttaattgtac aatataatgt acaattgttt tatagaaata aataaggggt gaaaggaatg
     1261 gaagcagtag cttattcaaa tttccgccaa aatttacgta gttatatgaa acaagttaat
     1321 gaggatgctg aaacacttat tgtaacaagt aaagatgtag aagatacagt tgttgtatta
     1381 tcaaaaagag attatgattc tatgcaagaa acgttgagaa cactttctaa taattacgtc
     1441 atggaaaaaa ttcgtcgagg agatgaacaa ttctccaaag gtgcatttaa aacacatgac
     1501 ttaatcgagg ttgaatctga tgattaaggc ttggtctgat gatgcttggg atgattatct
     1561 ttattggcat gagcaaggaa acaaaagcaa tataaaaaag attaacaagt taataaaaga
     1621 tatcgatcgt tccccctttg ctggattagg aaaacctgag ccattaaagc atgatttatc
     1681 tggaaaatgg tccagaagaa ttacagatga acatagactg atatatagag ttgaaaatga
     1741 aacgatattt atttattctg caaaagatca ctattaacca atcggaagta aggaaagggt
     1801 cagaaactta aaagtttttg atccttattt tatttaccct agtcatttaa aaagctaata
     1861 tagcttagtg ttgattgtta ttaatgaatg tgtttgttac gcgtattacg gatataaggt
     1921 tagtaaaatc atttct
//
LOCUS       DAXWLH010000274         1928 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXWLH010000274 DAXWLH010000000
VERSION     DAXWLH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.281"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(308..381)
                     /locus_tag="ACGE36_002760"
     tRNA            complement(308..381)
                     /locus_tag="ACGE36_002760"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Ile,seq:cat)
     gene            complement(570..1589)
                     /locus_tag="ACGE36_002761"
     CDS             complement(570..1589)
                     /locus_tag="ACGE36_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8385101.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1600..1806)
                     /locus_tag="ACGE36_002762"
     CDS             complement(1600..1806)
                     /locus_tag="ACGE36_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8385102.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          566 a          391 c          328 g          643 t
ORIGIN      
        1 gctttttaat ggcttcaaat ataaaagagt ttaaagcgta acacacttat ggggaagtgg
       61 tttggggtgc gctttaaact cttctttatt attatctcac aatttaaccc aaatgtcttt
      121 ctatttaaaa atcaaagtaa aacttttcaa atatacagaa gtataactat gtgaaacatc
      181 ctttcattaa tccataaaag gatacataaa aaagccactc atttgagtgg caatgaagaa
      241 aagctttagc ttgtataata ctcttcaaaa aaattctaac acagaacgat tcaaatggct
      301 acgttaatgt accctgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct
      361 gaccaactga gctaagggta ctggttgttg ccacataaag ccataaacaa tcaaccagta
      421 gaatgtgtgg caacaaacct gttatcgcat agcttggagt gtgactatta tgggtgatag
      481 tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt caatatagtt
      541 atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa ataccaacgt
      601 ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt cgttgcccgc
      661 atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac atgatagcca
      721 acatttccag aagtattata agcttgattt acatctgatc tataaattcc atcagctctt
      781 actcgagcta attctttccc agtattgtaa tccataatga agatatactc gtatttatag
      841 ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc aaactgatca
      901 atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc tcgtgttgga
      961 tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc atgagctaaa
     1021 tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta aggatctgta
     1081 tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg ttggttataa
     1141 ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc caattcaacg
     1201 tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa ctcaatctgc
     1261 acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc aggttgaccg
     1321 acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct agtccatgaa
     1381 cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt ttcatgcagt
     1441 aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa attaaattca
     1501 ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt taacaggctc
     1561 atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg atgaaatttt
     1621 cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa aacctgtaat
     1681 aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa gaccgacaaa
     1741 agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa aggttctatt
     1801 gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat cgcttgtttt
     1861 cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc agttgttcaa
     1921 ttttatct
//
LOCUS       DAXWLH010000275         1910 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXWLH010000275 DAXWLH010000000
VERSION     DAXWLH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.282"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            287..904
                     /locus_tag="ACGE36_002763"
     CDS             287..904
                     /locus_tag="ACGE36_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385103.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            945..1244
                     /locus_tag="ACGE36_002764"
     CDS             945..1244
                     /locus_tag="ACGE36_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFF8385104.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1278..1445
                     /locus_tag="ACGE36_002765"
     CDS             1278..1445
                     /locus_tag="ACGE36_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8385105.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1490..>1910)
                     /locus_tag="ACGE36_002766"
     CDS             complement(1490..>1910)
                     /locus_tag="ACGE36_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385106.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          634 a          308 c          375 g          593 t
ORIGIN      
        1 tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg ataactagtt
       61 tatttaatgg cagaccgtta aggcctgcca tttttatgta ttttagcgga ataaatataa
      121 tcatgcggga gtgtaaactt actgtttcaa aaagtatact ttttgacatg ccttgatttt
      181 ctttgtttat gtatggcata ataaccacat aatacaattt gaaacgtttc tgatttaatc
      241 tgattgtttt gaaacaattt tagtgaaaaa agggtagata aaaacgatga gaaaattttt
      301 ttatatccgg gtcagtagtt atgatcaatc gatcgaacga caactgattt ccgcgaagga
      361 attaggaatc cctgaagaat atatatttgt agaaaaagcc agcggcaaag attttaaacg
      421 ccctgaatat caattaatga aacggatgtt tcgaaaagga gatttactct atctacaatc
      481 attagataga ctaggcagaa ataaacaaat gattttagaa gaatggcgag aattaactaa
      541 ggaaaagcag attgatattt ctgtgttaga catgcctctg ttagatactg ctagttacaa
      601 gcaactagat gggttagaga cgcttatctc tgacttaatt ctgcaattac tcagctatat
      661 ggccgaagac gagcggaaac gaattaatag ccggcaaaga gaaggcgtag aagctgcgaa
      721 acaaaagggt gtaaaattcg gcagaaagaa aattgcttta cctcctgaat ttcctgcgat
      781 ctattctgac tggaaagcag gaaagataac tgcagtctat gctatggatc aattgggcat
      841 gaaacgaaat accttttatc gacgggtgaa agaatatgaa cataccttgc accacttttc
      901 ttgatagata taacagctat tagacgggaa gggagagttt gaaaatggac ttaacgaatc
      961 aaagggtcgt ttttactgga acgctgcagc aaatgacacg aacccaagcc atcggtttgg
     1021 ttcactcttt aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat ttcctggttt
     1081 gtggtcagta ttctaagtcg ttgtttgatg attctctcta tacaaaaaag gaacaaaccg
     1141 cgcgggactt gattgcgtta ggtcatgaga ttaccatttt atcagaagtc gaattttatg
     1201 cgttaattag tcaacagttg aaagaatggc aacggtattt ataatcgtta gtaaaattta
     1261 caaggaggaa aatttttatg aagcgaaaat cttatgatcg ttggggcatt gaaataacta
     1321 gttttaatcg aaatattgct tttcacgtgg agtgtgactg tggggaatta gcaaaaagtg
     1381 tttatcaaaa aaattacttt tactgtccta aatgccatcg ccgatacggg gtacatggaa
     1441 catagaaacg aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc tacaaatcat
     1501 tcgctttctt tattttataa atagttggtc gagagatccc gttttctttt gcaatctctg
     1561 caactggaat gccttgcttt aacatgtcca ccacggtttt aaaaactaag cgtttttgcg
     1621 gatctgttga gttaatagaa tactcgattg gtcgtccctt atatcggccg gctttttgag
     1681 ccaataatac tccttgccgc tgacgttcac gaattttctc acgctcgttt tgtgcaatat
     1741 aacttagcag tgataaaaac atatcgaaca tagcggtatc caggagttca tttccagtat
     1801 taaaatctaa aaactgtgca tctaatattt ttaacgctac ttttttctgt ttcataaaag
     1861 caactgtatt ttttatatct tcataatctc ttcctaaacg atctagtgat
//
LOCUS       DAXWLH010000276         1892 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXWLH010000276 DAXWLH010000000
VERSION     DAXWLH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.283"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            286..882
                     /locus_tag="ACGE36_002767"
     CDS             286..882
                     /locus_tag="ACGE36_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8385107.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(940..1422)
                     /locus_tag="ACGE36_002768"
     CDS             complement(940..1422)
                     /locus_tag="ACGE36_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385108.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1528..1725)
                     /locus_tag="ACGE36_002769"
     CDS             complement(1528..1725)
                     /locus_tag="ACGE36_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385109.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          649 a          369 c          286 g          588 t
ORIGIN      
        1 aaaagcaagt aatttttaat ggcaatatca taaaaaaaag aagatagaga tttaataatt
       61 atatatcagc tgtttttttc aaataagcaa aaattcctaa aacacaacaa tgaaagcgat
      121 aactaagaaa atccttacat aacgattttt cttattaaaa ttgttttttg aacttcgctt
      181 attttttgtg agcatatctg tttacttact ttttgtaagg tgctatactt catttcgtaa
      241 tcaattaaaa aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac aacttttact
      301 gacactttga aaaaccgccg ttcaatctat catctaggac gtaatgtttc actttctaat
      361 gaagaattaa caactttgat caaagaagca attaaagaaa gcccaactgc tttcaatgct
      421 caatcaacac gcgcagttat ccttttcgga gatgcacatg aaaaactttg ggaaatcact
      481 gaagaagcac ttcgcccatt gactccagca gaagctttcc caaatacaca aaacaaatta
      541 gctggtttca aaaatggtta tggtacagta ttattcttca aagatactga cgtggtaaaa
      601 ggtctacaag aacaatttga attatatgca gacaacttcc cagattggtc agaacaatca
      661 aacggaatcg caacagctaa tacttgggta gcattagttg ataaaggatt gggcgcaaac
      721 ttgcaacatt acaacccagt tattgacgaa gcagttgcaa aagaatggaa tatcccttca
      781 aactggaaac tacgttcgca attagtattc ggttcaccag aaacaccggc tggggaaaaa
      841 gaatacatga acgatgccga tcgcttccgc gtatttggat aattttattt gaaataaaaa
      901 acagaggttg tgacaaacat cttgtcgcaa cctctgtttt tattctggat caatcataat
      961 atcaatctct gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt cttctaatga
     1021 tgcagaaaat ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca tttttacgat
     1081 tttagaacct tttggctgat ataatacaat tttattgatc tcagaaaaag gaacaccttt
     1141 tttgtcaaag ctattcaaaa caagtccttc ttctgtgatt cctctactgt ccaacaaaaa
     1201 actaataaaa atcattgctg acaagatccc ttttgccaat tcatctgctg taggatgact
     1261 gatgagtgta taccaaatga ctagtgacac taagaagggt atcaccaagt agacccatcg
     1321 atttcgcaca gtccgataaa taatttcttt tcgaaccagc caaagaaata aaaacatcac
     1381 aacaacgaat aaaaacaata gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt
     1441 ttcttctcca gaaaatagga aaagaatact gatttacctt ctcaaagaaa ttggacttgc
     1501 agcacgccgc acgtccaatt gttttcttta ttcaaatgta tcatattttg caacaaccat
     1561 gcttgctttt tcatctaata attcttggtc ctctgcagca cattgggcaa ctttgaccac
     1621 ggcgcagttt gtcatttttg atacaacttc accaatgacc gtttttgtaa aaccaattgg
     1681 tttgcatgca tattgttctc ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa
     1741 ctgctggaac ttttctccac attaccactg ctatttcttt ggagatttaa ctcattgatt
     1801 tttatttaca gttaaaaatc aaccttacag gcattatagc gtactgtttt cgttttcaca
     1861 attaaaacag ctgttgtatt tagaagaatt tt
//
LOCUS       DAXWLH010000277         1873 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXWLH010000277 DAXWLH010000000
VERSION     DAXWLH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.284"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            35..577
                     /locus_tag="ACGE36_002770"
     CDS             35..577
                     /locus_tag="ACGE36_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385110.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            733..1182
                     /locus_tag="ACGE36_002771"
     CDS             733..1182
                     /locus_tag="ACGE36_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFF8385111.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1212..>1873)
                     /locus_tag="ACGE36_002772"
     CDS             complement(1212..>1873)
                     /locus_tag="ACGE36_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFF8385112.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          676 a          294 c          366 g          537 t
ORIGIN      
        1 atatttcatc aacgtttcat aaaaggtgtg tagaatgtta ttgaaagtag ctataaaatg
       61 ctattgtgta aaaaataaag gaggaataat ttatgaaaaa atagtaccaa gaggagcatg
      121 cctattactt gcattatgtt tgagcttaag cgcagttggg tgtcaaaaaa gtaatgaaaa
      181 caataaaata aaagaaggac aatccataag ttctgaagag gaaggcatgt caattaatgc
      241 aaatggcaaa aatgaaactt ttaagccatc agattataca ttggaggcaa agaaagaata
      301 tgtttatgaa tatttagggc ttaaatttaa actctcagac aaatttagaa attatatagc
      361 tgataaaaag atagcgatgt tagatgatca aagtcctata gataaagaac taaagtatgc
      421 tattttgaca tttgagaaaa tgacagaaga acaaaagaat gcagttatag aaaagatggg
      481 cgatggatat aaaaattggc aaaatgagct tgaaagaatt gggactattg gtatatttga
      541 aaaaaataca tcagaagaaa aaaatctaaa attataaaat gtgatactca tactaaaata
      601 ggagtttcaa gtgatggaaa atacgattgc tatttaagca caaatagcgg agctgaaagt
      661 aatcttctgg atgaattaaa aagaactgag attcaaatta tagataaaaa agaacgtcct
      721 aaaaatggtt ttgtgctatc agaaaaaact gatttagaaa atacagaagc atttaacaaa
      781 gaatcggtta aagatttacg taaactacca acaaaggaca taaatggcaa ggattttaca
      841 agtaaagatt ttgagaagta tgatcttact atggtaaatg tatttgcaac ttggtgtaca
      901 gcttgtgtaa aggaaattcc tgatttggtt gaggttcaga atgaaatgaa aagcaagggt
      961 gtaaatatcg ttggagttgt tacagatcct gttgatgata acggtgaaaa caaggaagca
     1021 attgaaaagt caaagttaat acatgaaaag acaaaggctt catatccttt cttaatgcca
     1081 gatggaatgg caacactttt tttgacaaaa attataaggt gttgtgaatg ctgcggtgtt
     1141 atggctacga ttgcccttga caatgagcat cctcggatct gattttctcc ggcagaagca
     1201 caatcattca attacgctgc ctctccagag gtatcagatc cgcgctcatt atcaaggatt
     1261 cgcttcgcca atctctgttg tcgaatttgg atcggtgttt catacgccaa cgtgccatgt
     1321 aagcgtagat agttccacca atgcacataa tcaaacaatt ctagacgtag ctgtgccagt
     1381 gtctcaaatt ggtattgatg cacgaattct actttgacag atttataggt tgattccaca
     1441 acggcattat catacggaca acccttccta ctcaatgaac gagtaattcc aaaaccattc
     1501 agaatctcat gaatggtttg gttatcaaat tcctttcccc ggtctgtatg aaaaagcttg
     1561 acgtctgtta aagaatacgg tatccgtcca aaggcttctt ttaccaatga ggcatctttc
     1621 ttctcaccac aggaataacc aataatttct cgattaaaca aatcaagtat taagcagata
     1681 taatgccact ttttccccac gcgaacataa gtaagatccg taacgatggc ttccaatggc
     1741 tgttcttgtg taaaggtccg atctaataca ttcgctgttt tcgcttcatt acaagctgtt
     1801 cgttgccctt taaaatgagc gatcgtatag gtagatgtca atccgcggcg tttcatgatt
     1861 cgaccgattc ggc
//
LOCUS       DAXWLH010000278         1842 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXWLH010000278 DAXWLH010000000
VERSION     DAXWLH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.285"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ACGE36_002773"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ACGE36_002773"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="ACGE36_002774"
     tRNA            207..279
                     /locus_tag="ACGE36_002774"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="ACGE36_002775"
     tRNA            283..355
                     /locus_tag="ACGE36_002775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="ACGE36_002776"
     tRNA            363..444
                     /locus_tag="ACGE36_002776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="ACGE36_002777"
     tRNA            462..534
                     /locus_tag="ACGE36_002777"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="ACGE36_002778"
     tRNA            546..617
                     /locus_tag="ACGE36_002778"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="ACGE36_002779"
     tRNA            634..719
                     /locus_tag="ACGE36_002779"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
     gene            730..803
                     /locus_tag="ACGE36_002780"
     tRNA            730..803
                     /locus_tag="ACGE36_002780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Arg,seq:acg)
     gene            813..886
                     /locus_tag="ACGE36_002781"
     tRNA            813..886
                     /locus_tag="ACGE36_002781"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Pro,seq:tgg)
     gene            906..978
                     /locus_tag="ACGE36_002782"
     tRNA            906..978
                     /locus_tag="ACGE36_002782"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Ala,seq:tgc)
     gene            1006..1079
                     /locus_tag="ACGE36_002783"
     tRNA            1006..1079
                     /locus_tag="ACGE36_002783"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:Met,seq:cat)
     gene            1093..1166
                     /locus_tag="ACGE36_002784"
     tRNA            1093..1166
                     /locus_tag="ACGE36_002784"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ile,seq:cat)
     gene            1190..1279
                     /locus_tag="ACGE36_002785"
     tRNA            1190..1279
                     /locus_tag="ACGE36_002785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1226..1228,aa:Ser,seq:tga)
     gene            1292..1365
                     /locus_tag="ACGE36_002786"
     tRNA            1292..1365
                     /locus_tag="ACGE36_002786"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Met,seq:cat)
     gene            1384..1459
                     /locus_tag="ACGE36_002787"
     tRNA            1384..1459
                     /locus_tag="ACGE36_002787"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Asp,seq:gtc)
     gene            1461..1531
                     /locus_tag="ACGE36_002788"
     tRNA            1461..1531
                     /locus_tag="ACGE36_002788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Gly,seq:tcc)
     gene            1545..1618
                     /locus_tag="ACGE36_002789"
     tRNA            1545..1618
                     /locus_tag="ACGE36_002789"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Ile,seq:gat)
     gene            1643..1730
                     /locus_tag="ACGE36_002790"
     tRNA            1643..1730
                     /locus_tag="ACGE36_002790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Ser,seq:gct)
     gene            1752..1823
                     /locus_tag="ACGE36_002791"
     tRNA            1752..1823
                     /locus_tag="ACGE36_002791"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Glu,seq:ttc)
BASE COUNT          420 a          431 c          507 g          484 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc aaaaggttag
      781 gggttcgact cctcttgggc gcgttaatta aacgggaagt agctcagctt ggtagagcac
      841 ttggtttggg accaaggggt cgcaggttcg aatcctgtct tcccgataat tataaattta
      901 aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt
      961 cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg tgtagctcag
     1021 ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct ccgccgcgat
     1081 ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata accgtccggt
     1141 cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg gaggattacc
     1201 caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc gtgcaagggt
     1261 tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg gtagctcgtc
     1321 gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa aactaaaaac
     1381 actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat cgcgggttcg
     1441 attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc cttccaagct
     1501 gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct atagctcagc
     1561 tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt
     1621 agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc gcccctgcta
     1681 agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg tttcaatatt
     1741 ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg gcggtaacac
     1801 gggttcgaat cccgtacggg tcattaattt aatatagttt ta
//
LOCUS       DAXWLH010000279         1825 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXWLH010000279 DAXWLH010000000
VERSION     DAXWLH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.286"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(85..234)
                     /locus_tag="ACGE36_002792"
     CDS             complement(85..234)
                     /locus_tag="ACGE36_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385113.1"
                     /translation="MKKKVIPYCPKPKNAFERGLFKYFEGKKGQDHKEENQKKKSKKK
                     SEEFF"
     gene            735..1076
                     /locus_tag="ACGE36_002793"
     CDS             735..1076
                     /locus_tag="ACGE36_002793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385114.1"
                     /translation="MKANTPYKVGDIFYSSWGYEQTNVNFWQIIKLTEKTAWFRPIKK
                     QTVKTYTSMSGETMPIPNEFIDYPLVRTKDSIVRKRINPALYPNELYGNRSWDTFYRY
                     DNQPVYESSYY"
     gene            1276..1569
                     /locus_tag="ACGE36_002794"
     CDS             1276..1569
                     /locus_tag="ACGE36_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385115.1"
                     /translation="MLRTNAIPIVDSYDRNTNNYLGSFEQTDENILNYVAGLSPFQSV
                     RLVEHTTDTLILTTIGYFFDQVSDQQWLQQILPKLIAKQTGKKTIEAVKIFFY"
     gene            1624..>1825
                     /locus_tag="ACGE36_002795"
     CDS             1624..>1825
                     /locus_tag="ACGE36_002795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385116.1"
                     /translation="MAILQPFGEPIERTEFIQHYMKLFVQVIKHTHQIDEFYSKEIEY
                     LLAEKQKIALLYDYFVEMYDRAP"
BASE COUNT          660 a          332 c          268 g          565 t
ORIGIN      
        1 atttacagaa aaacgacctc tttaaaaata aaaggtacgg gttactgatt acttgtaaat
       61 ctttacgttc cagttttatt ctttctaaaa aaactcctca ctttttttct tagacttctt
      121 tttttgattc tcctctttat ggtcttgtcc ttttttgcct tcaaaatatt taaacaaacc
      181 tctttcaaaa gcatttttag gttttggaca ataaggaata acttttttct tcatataaac
      241 acctcattta actatttgat ctattttata tcaccacatt atcaaaataa caaatgtatt
      301 aaaagaaagt ttttattaaa accatcttga ccttttgaga aatttcaatg cgatttcttt
      361 ttattcccat actttttttg ttggtcactt tcatataccc aatctaagcg atcatcggcg
      421 acggtgcttt tacaacggat ggtaagacaa aacaaggggc ggaaaaactt ttttgatcat
      481 ttcataaaaa actttttcct acagcattcc cttgttttct cttaccacac gttgtgaacc
      541 caaagcacgc cgacgaacgc ttagctatac gaaaatgacc aaccaaaaac aaaaagtgag
      601 gtgaatagaa acaaaaagaa attgaaatct cccaaaaggt cgcccacctc ggagacaact
      661 tgctgccgaa aatggttggt tgattcggct aagtatcaaa taaggcaaat caaacattat
      721 tggaggaata gatcatgaaa gcaaatacac catacaaagt aggagacatt ttttatagta
      781 gttggggcta tgaacaaacc aacgttaatt tttggcaaat tattaaatta accgaaaaaa
      841 cagcatggtt tcgacccatc aaaaagcaaa ctgtaaaaac ctatacttct atgagtggag
      901 aaactatgcc aataccaaat gaatttatcg attatccgct tgtacgaacc aaagacagca
      961 ttgttagaaa acgaattaat ccagccttat atcccaatga attatacggt aatcgcagtt
     1021 gggatacatt ttaccgctat gataatcaac cggtttatga aagcagttat tactaagagg
     1081 gaatttccct cttagtcaat gttaaaaata acaaaaataa aaggagcaag aacaatgaaa
     1141 ccaataactc tatatgaaat cttaggtgaa gaacggtctg acaatttacc aacaacgcat
     1201 aaattaagag ttcttgcttc taaatctatc aataaaaaat gataactgag tatttctaat
     1261 caaggagaga ttgcaatgct aagaacaaat gctattccta tcgttgattc ttacgatcgg
     1321 aataccaata actatttagg atcttttgaa caaacagatg aaaatatttt gaactatgtt
     1381 gctgggttaa gtccttttca atctgtacgt ttagttgaac atacaacaga tacactcatt
     1441 ttaacaacaa tcggctactt cttcgatcag gtatctgacc aacaatggtt acaacagatt
     1501 ttacctaaac ttatcgcaaa acaaactgga aaaaaaacca tcgaagcagt caaaatattt
     1561 ttttactaaa tacatgtatt cattctcaag ggatttttcc cttgagtgaa aggagaaaga
     1621 atgatggcca tacttcaacc ctttggtgaa ccaatcgaac gaacagaatt cattcaacac
     1681 tatatgaaat tatttgttca agttattaaa cacactcatc aaattgatga gttttattcc
     1741 aaagaaatag agtatcttct agcagagaaa caaaaaattg ctttacttta tgactatttt
     1801 gtcgaaatgt atgatcgcgc accag
//
LOCUS       DAXWLH010000280         1810 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXWLH010000280 DAXWLH010000000
VERSION     DAXWLH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.287"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..722)
                     /locus_tag="ACGE36_002796"
     CDS             complement(<1..722)
                     /locus_tag="ACGE36_002796"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8385117.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="ACGE36_002797"
     CDS             complement(727..1494)
                     /locus_tag="ACGE36_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8385118.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
BASE COUNT          574 a          288 c          307 g          641 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt
//
LOCUS       DAXWLH010000281         1796 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXWLH010000281 DAXWLH010000000
VERSION     DAXWLH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.288"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            44..562
                     /locus_tag="ACGE36_002798"
     CDS             44..562
                     /locus_tag="ACGE36_002798"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8385119.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            690..1373
                     /locus_tag="ACGE36_002799"
     CDS             690..1373
                     /locus_tag="ACGE36_002799"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFF8385120.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1682..1755
                     /locus_tag="ACGE36_002800"
     tRNA            1682..1755
                     /locus_tag="ACGE36_002800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Arg,seq:tct)
BASE COUNT          597 a          287 c          371 g          541 t
ORIGIN      
        1 tatatgatta agaaggattg aataaagaaa gaagttgaaa acgatacctg attacaaaaa
       61 aataactttt ataagttcta taacgttgat tatattttta attcttattc ttctcgtcgt
      121 cttggttccc cgaatttttg gtttgactcc ttatattatt gaggacaaag aaatgagtcc
      181 atattatgca caaggaaatc tgatatatgt acgaccgaaa aatacaaatg agattttagt
      241 cggagatatt attacatatt atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat
      301 agccgttgaa aacaaccacg aagattttta tacaaaagct gatacaaaga cttatattga
      361 gcctggagta gtaaaaaaga gaaatattat cggtagtccc atctttcaga ttccatatat
      421 tggactagtc ctttcaaaaa cggcatttgc atggattcag gggttattct ttacgattgc
      481 tttttgccta acaggaatca cagcatggaa tacctttgta gagttgaaaa agaagcggag
      541 acgaacacag aaatttttgt aagaatgtta aaaacataaa tgtattccag agatccaacg
      601 actcttctga acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt
      661 tcgtataatg tgtgaagagg aggagtagta tgcgcaatga aatgatgcat cgtcctgtag
      721 tcaaagaaga actattggat tttatgcgta cccagcaaaa aaaattaccg ggtgagctag
      781 gcaaactgga agaagaagct catgtagcag aagtaccagt tattccccat gaaacagttg
      841 tatttttgaa atttttactt ggacagcttc agcctgaacg aatcttggag atcggagcag
      901 ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct catgttacga
      961 caattgatcg tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa cgtctaggac
     1021 tgacagataa agtgactttg cttgaaggtc aagcagcaga tatcttgccg gagttgtctg
     1081 gtccgtatga ctttattttt atggatagtg caaaatcaaa atatattgaa tttttacctg
     1141 aatgcttacg tttgttaaga aaaggtggcg ttttgatggt agatgacatc ttccaaggag
     1201 gaacgatctt gctcccagat gaagagattc cccgtggcaa acgagctatc catcgtaaat
     1261 taaatgaatt tcttcgtgtt gtgatggatc atcccgattt gacatcgaca atcttgccat
     1321 taggtgatgg tgtgatctta atgacaaagg aatcagaaac aatcgagttg tgatacatga
     1381 ttatggcagc ctgtagataa tcgattgaat cgagggctca attcgaactt gatgtaaaag
     1441 tttctatatt tgtaagtcaa ttaaaggata caggaaatct aaataaacag gagtagcatg
     1501 aagtcaatgt agctcctgtt ttcaattgat aaccttagaa cagctgaagg agaatgtctc
     1561 gttttaaaag attgatttag tagttgggcc aaaataattc attatttgct aattaaataa
     1621 ctatttcgta ttgacagaag caatttaatt atctataatt attgatgttc cgctgattct
     1681 tgtcatagta gctcagctgg atagagcatc cgccttctaa gcggacggtc gggggttcga
     1741 atccctcctg tgacgctaaa aaaccgttag aaactgaggt ttctaacggt ttttct
//
LOCUS       DAXWLH010000282         1735 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXWLH010000282 DAXWLH010000000
VERSION     DAXWLH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.289"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            146..1564
                     /locus_tag="ACGE36_002801"
     CDS             146..1564
                     /locus_tag="ACGE36_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8385121.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIDGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          545 a          267 c          339 g          584 t
ORIGIN      
        1 tcttatgcat caatgttttt agattatttt ttgtttttta tctgtcaaga taatgcaata
       61 aaaagccaac attgtgaata taaaaacaag aaaaaaagag ttataatttt gaaaacgttt
      121 actagaagag gtaggaggaa ttcaaatgct ttcacaaaat gctgttacag caaatacaat
      181 aaaaaaagaa aaaaaggaat ttgggatttc cgataagata ggctacatgt tcggtgatct
      241 aggtaactgt tttattttag gtttagtcaa tagtttcttg atgatttatt atacgaatgt
      301 tcttggcgtt tcaggggcaa tcgtaggtac gttattcttg atttcgagaa ttttggatgc
      361 gtttgctgat gtgatggttg gaagattatg tgatgtctca gagttaacag aaactggccg
      421 atttattcct tggatcagaa aaatgaagta tccattttgc ttagtgactg tcattttgtt
      481 tttgccattc gtccaacatc ttccaatgac ggtgaagatt gcttatattt ttgtttcata
      541 cattgtttat gggatttttc tctcaacaat caatatcccg tatggagcaa tggcagcagc
      601 aattagctca aatcctaatg accgcacctc gttgtccacg ttccgaagca ttggttctgc
      661 gattgacgga tcaactacag gattcttcat tccgatttta atgtataccc ataacaagag
      721 tggacaacaa gtcgtttcag ggaatcattt cttttggatt tcaatcggat gtgctgcaat
      781 agcatttgtc gcatatatgt taacttgtaa attgactact gaaagagtaa gagttgtcaa
      841 aaacgaaaaa gtatctgctt ctcagttggt aaaaggtttg ttagctaatc gagcactgat
      901 tattttagtc attgttgaca ttttcattgt gatcaatcag attttagctg ggacgaatat
      961 gacttattta ttcaatgatt acttccataa taaacaagca atgtctgttg ctcttttatt
     1021 tacttacgga acagtagtag ttttagcacc atttgctaca aagctgacaa aattatttgg
     1081 taaaaaggaa gcaagcgtat ttgcgttact tgcttcttca tgtatgtact tgctgatgta
     1141 tttccttcat ataacaaatg catgggtata tctgattctt ttatttatcg ctactttagg
     1201 atctggttta ttcaatttga tggtctgggc attcattact gacgtgattg attatcatca
     1261 atataagaca ggcttaagag aagatggaac agtatatgga gtaaattctt ttgcgagaaa
     1321 agtaggacaa gcatgtgcag gagcggtagg cggatttatg ctggcactca ttggttacca
     1381 atcgtctaca acaggtggag ccatccaatc agttttagta caagaaagga tttatacgat
     1441 ggcaaacttg ttaccagcat tctgcttatt tgtatccgca atcattttgc tagtaggtta
     1501 cccactgaat cgttcaaaaa ctgtatttat tggtgaagaa ttgaaaaaaa tcaatgaaag
     1561 atagggagaa acgtattgcg gacaaaaaaa gggattatca atgctttgaa ctttaaaaca
     1621 aacgacactc aacaagatga tacaataata ctgcaaaaag caatcgatca aggtgcgaaa
     1681 gaacacttac ctgtatttat tccaaaaggc atttatttag tcggagccct ttttt
//
LOCUS       DAXWLH010000283         1734 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXWLH010000283 DAXWLH010000000
VERSION     DAXWLH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.290"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..1087)
                     /locus_tag="ACGE36_002802"
     CDS             complement(<1..1087)
                     /locus_tag="ACGE36_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8385122.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKR"
     gene            complement(1197..>1734)
                     /locus_tag="ACGE36_002803"
     CDS             complement(1197..>1734)
                     /locus_tag="ACGE36_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFF8385123.1"
                     /translation="ATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISY
                     NYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVAS
                     WALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVAS
                     LSMIGVLIGRKVLKKYKK"
BASE COUNT          634 a          326 c          239 g          535 t
ORIGIN      
        1 ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa
       61 agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta
      121 gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt
      181 caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata attccactta
      241 aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa
      301 aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa
      361 caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg
      421 ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc
      481 cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa
      541 taaaatttgt attctctgtc attttcttta acaagaatac accaattact aacatggcga
      601 ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg
      661 ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg
      721 cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac
      781 ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa
      841 ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa
      901 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
      961 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
     1021 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
     1081 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
     1141 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
     1201 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
     1261 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
     1321 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
     1381 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
     1441 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
     1501 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
     1561 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
     1621 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
     1681 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agct
//
LOCUS       DAXWLH010000284         1699 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXWLH010000284 DAXWLH010000000
VERSION     DAXWLH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.291"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(139..516)
                     /locus_tag="ACGE36_002804"
     CDS             complement(139..516)
                     /locus_tag="ACGE36_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385124.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(816..1697)
                     /gene="galU"
                     /locus_tag="ACGE36_002805"
     CDS             complement(816..1697)
                     /gene="galU"
                     /locus_tag="ACGE36_002805"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8385125.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          477 a          377 c          247 g          598 t
ORIGIN      
        1 atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt
       61 tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt
      121 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
      181 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
      241 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
      301 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
      361 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
      421 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
      481 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
      541 attaaaaaat tcgctttttt attctactat gaatcttctg gcttggcttt catttctctt
      601 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
      661 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
      721 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
      781 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
      841 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
      901 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
      961 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
     1021 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
     1081 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     1141 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     1201 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     1261 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     1321 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     1381 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     1441 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     1501 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     1561 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     1621 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     1681 gcttttttga ccttcactt
//
LOCUS       DAXWLH010000285         1672 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXWLH010000285 DAXWLH010000000
VERSION     DAXWLH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.292"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            230..>1672
                     /locus_tag="ACGE36_002806"
     CDS             230..>1672
                     /locus_tag="ACGE36_002806"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8385126.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          568 a          316 c          328 g          460 t
ORIGIN      
        1 atttaacaag gttattcgaa ccttatttta agttacttat tatttaatgt atcgaaaaag
       61 gtgaaatagg tttgttataa taagaattaa gtaagacatg aattgtctaa gcaaatgtag
      121 cttatccatt tcattaaaat acatatggtg aaaaaacaag ttcaaatagt aactaatttt
      181 ttaaaaaact gatttataaa aaatacaaca cgaatgagga aaataaccaa tgacagaaca
      241 aatcgaaaaa aattcaaaaa gcttatatca ggcactgtgg aattccgcag atatcctgcg
      301 ttcaaaaatg gacgctaatg aatacaagag ctatttacta ggcttggtct tttataaata
      361 cttatccgac aacatgctgc ggtatgtctc cgttctattg gaggaagaaa cggacgacct
      421 aaataaagca caagaccttt acgttgaagc atttaaagat gcagatataa aggatgatct
      481 cttgtatgaa ctaaaagatg agttttcata taccatcgca cccgcattga cctttaccgc
      541 gcaagtggca gccattcatg atggatcttt tcaattagaa gacctggtac aaggtttccg
      601 tgacattgaa caatccagcg aaaactttga gaacttattt gaagacattg atctgtattc
      661 taaaaaactg gggtcaaccc cacaaaaaca aaataaaacc atcgcagatg ttatgaaaga
      721 attagaaggg ttaaacatgg cggggcatgc tggcgatgtc ttaggcgatg cctatgagta
      781 cttaattggc caattcgcat ctgaatccgg taaaaaagcc ggtgaattct ataccccaca
      841 accggtcgca aaattgatga cacaaattgt tttacaagga aaagaagata aaaaaggctt
      901 ttctgtttac gacgcgacca tgggttcggg ctcgttactt ttgaacgcta aaaaatattc
      961 ccatcaaccg ggaaccattt cttatttcgg acaagagttg aatacgtcta cctataattt
     1021 ggcgcgtatg aacatgattt tgcatggggt tcccattgcc aaccaacact tgcataatgc
     1081 ggataccttg gatcaagact ggccaacaga agagccaacg aacttcgatg gggtgctcat
     1141 gaacccaccg tattctgcaa aatggtcggc tgataaaggc ttcttagatg acccacgctt
     1201 ctctgcctat ggcgtattag cccctaaatc aaaagcggac tttgctttct tattacatgg
     1261 ctactatcat ttgaaagata cgggcgtcat ggccattgtt ttgccacatg gggtgctctt
     1321 tagagggggc gcagaaggga agatacgcaa agccttgtta gaaaatggtg ccattgacac
     1381 cgtcattggc ttacctgcta acatcttctt taatacgtcc attccaacga ccgtaattat
     1441 cttgaagaaa gaccgtgcga aaaaagatgt gctctttatt gatgcgtcac aagattttga
     1501 aaaaataaaa acgcaaaata cgctacgaga ttaccatatt gacgctattt tagaggccta
     1561 taaaacccgt acaattaaag acaagtatgc ttatgtcgca gaatttgatg aaattgtgga
     1621 gaatgattac aacttaaaca ttccccgtta cgtggatacc tttgaagaag aa
//
LOCUS       DAXWLH010000286         1670 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXWLH010000286 DAXWLH010000000
VERSION     DAXWLH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.293"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..148)
                     /locus_tag="ACGE36_002807"
     CDS             complement(<1..148)
                     /locus_tag="ACGE36_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFF8385127.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFS"
     gene            306..569
                     /locus_tag="ACGE36_002808"
     CDS             306..569
                     /locus_tag="ACGE36_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385128.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            593..781
                     /locus_tag="ACGE36_002809"
     CDS             593..781
                     /locus_tag="ACGE36_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385129.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(864..1049)
                     /locus_tag="ACGE36_002810"
     CDS             complement(864..1049)
                     /locus_tag="ACGE36_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385130.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1264..1635
                     /locus_tag="ACGE36_002811"
     CDS             1264..1635
                     /locus_tag="ACGE36_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8385131.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT          614 a          226 c          323 g          507 t
ORIGIN      
        1 aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa actgaaaact
       61 tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat
      121 aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata
      181 caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct
      241 gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga
      301 ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga
      361 tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta
      421 ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc
      481 cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag
      541 catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc
      601 atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag
      661 agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt
      721 gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta
      781 gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga
      841 ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt tttgacactg
      901 tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc taattgttct
      961 ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat catttccttg
     1021 actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat caacatgatt
     1081 ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata tcaaataacg
     1141 atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa aatctccaac
     1201 ttgggacgga tggaatttct ttaactgacc catatactga atatataaca aggaggtaga
     1261 aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat tttgctattt
     1321 ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact tgatcagcta
     1381 aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga ggaagtaaat
     1441 gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg
     1501 gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa
     1561 ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga gaaagcatta
     1621 gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
//
LOCUS       DAXWLH010000287         1657 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXWLH010000287 DAXWLH010000000
VERSION     DAXWLH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.294"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1625
                     /locus_tag="ACGE36_002812"
     CDS             <1..1625
                     /locus_tag="ACGE36_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385132.1"
                     /translation="NRFYETYMQIKTGNFNFFQSLYSFQSSGRIITAFYGADFAYLQG
                     LLLIILKSWFKYQLVSSFSCFFIAGASMFWLTYKCKVRTNIGLIIALLYMSSSAVSYY
                     PIAQAFTGWGAALMPLLFVPAIEALKNKERPIRPLQLAIPVTLLLSTHLLSLVIGILA
                     ILPFYVIAFINTKNKLNMIFRLVESIGLTMLFSANTIIGFIDVYLSNKILSPSPISQM
                     LSQSMSFSLEGNSWGNYGLVFTAIFFAVIIYFFLNWAKTSLTSKVIVIVGAFFMLLSS
                     KLLPWNSIPHMFKFVSFFQFPQRFSVIAFVLLLLSFALILQESKLLKDVDKKYYILTL
                     LCALFSIFNVYNLMYDQSWHWNTNDPTAAGNNKSSMVEKDPQKLREAFYNKDLNIALK
                     AIQKGTPDYLPVQKNVEPSDVLKQNPYELYTNQIINNNVHFKKTVTSDSKLRLTWTNN
                     SNEESDIQLPIIIYNHSTVTLNGKKLTPNEIKTTQIGAAIVTSSPGKNTLVIGYKPFV
                     LFKIAFPIKILSILSTIIYVIYKYKKTKIIEI"
BASE COUNT          554 a          260 c          225 g          618 t
ORIGIN      
        1 ttaataggtt ttatgaaact tatatgcaga taaaaaccgg taattttaat ttctttcaat
       61 ccctgtattc ttttcagagt tctggcagaa ttattactgc tttttatggc gcagatttcg
      121 cttatttgca agggctactt ttaataatct taaaatcatg gtttaaatat caacttgtct
      181 cttctttcag ttgctttttt atagctggtg cttcgatgtt ttggctaact tataagtgta
      241 aagtacgaac taacatagga ttaatcattg ctttgttgta catgtcttca tcagcagttt
      301 cgtattatcc tattgctcaa gcttttactg gttggggtgc tgctttaatg ccgcttcttt
      361 ttgtgccagc aattgaagca ttaaaaaata aagaaagacc tattcgtcca ttgcaacttg
      421 caatccctgt aactttatta ctatctactc atttactttc attagtaatt ggaatattag
      481 ctattttacc tttttacgtt attgctttta ttaatacaaa aaataaactg aacatgattt
      541 ttcgcttagt agaatctata gggttaacca tgctattttc tgctaataca ataattggat
      601 ttatagatgt ctatctaagt aataaaatat tatccccttc tccgataagt caaatgttga
      661 gccaaagtat gagtttctcg ttggaaggaa actcttgggg aaattatggt ctagtattta
      721 ctgcaatttt ctttgctgta ataatatatt tctttttaaa ttgggctaaa actagtctta
      781 cttcaaaagt aatcgttatt gttggagcgt tttttatgtt actttcttcc aaattattac
      841 cttggaactc tatccctcat atgtttaaat ttgtttcatt tttccaattt ccacaaagat
      901 tctcagtcat tgcttttgtt ttacttttat tatcatttgc tcttatattg caagaatcaa
      961 aactacttaa agatgttgac aaaaagtact atatattaac tttactgtgt gctctatttt
     1021 caatatttaa tgtatataat cttatgtatg accaatcttg gcactggaat acaaatgatc
     1081 ccaccgctgc aggaaacaat aaaagttcta tggttgaaaa agatcctcaa aagttaaggg
     1141 aagcctttta taataaagat ctgaatattg ccttgaaagc aatccaaaaa ggaactcctg
     1201 actatttacc tgtacaaaaa aatgtagaac catcagatgt attaaaacaa aacccttatg
     1261 aattgtatac aaaccaaata ataaataaca atgtacattt taaaaagact gtaactagtg
     1321 attctaaact tcgtttaact tggactaata attcaaatga ggaatccgat attcaattgc
     1381 ctataataat ttataatcat agtacagtaa ctttgaatgg taaaaaatta actccaaacg
     1441 aaattaaaac tactcaaatt ggagctgcta ttgttacatc gagtccagga aaaaatactt
     1501 tagttatcgg ttataagcca tttgttttat ttaaaatagc ctttccaata aaaatattgt
     1561 ctatactttc aactatcatt tatgtcatct acaagtataa aaaaacaaaa ataatcgaaa
     1621 tataaaaatt acaaaacatt aatatgttat gtgatgc
//
LOCUS       DAXWLH010000288         1653 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXWLH010000288 DAXWLH010000000
VERSION     DAXWLH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.295"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..862)
                     /locus_tag="ACGE36_002813"
     CDS             complement(<1..862)
                     /locus_tag="ACGE36_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFF8385133.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYI"
     gene            complement(1021..1287)
                     /locus_tag="ACGE36_002814"
     CDS             complement(1021..1287)
                     /locus_tag="ACGE36_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8385134.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1277..1633)
                     /locus_tag="ACGE36_002815"
     CDS             complement(1277..1633)
                     /locus_tag="ACGE36_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8385135.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          470 a          321 c          202 g          660 t
ORIGIN      
        1 aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt aaaattcccg
       61 atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc cttaaattgt
      121 tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa aagatagaat
      181 tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat tctattggat
      241 caaaaatatc taagttgtca tcaaaatctt tatcagttcc ggtaactttt ttgttaatta
      301 ctgaaggtgt cttgtaagaa atagaatcta aatcaaagcg ttttgattct aaattatttt
      361 gtgctttctc aattaaataa ttcacttttt ttgtgagaaa ccttctgata ttgtctttgc
      421 tatatgttcc ataaaaaccc ttatcttcta aagagtccct gtacttctta acttctatat
      481 actcttcagc caaaacttgt ggaactggtt catctaagaa aaaaacccct acctcgcctt
      541 tgctattttt ataaaagtaa tcaatttctg ctatagttcc cgatctataa ttatcaactg
      601 gagaacccaa cttatggcca aaaatcgcta ataatatatc actattttct acaatttgtt
      661 tattaataac actttgccca gagctattat ttatataagt aggaatcgca ttcgtttccc
      721 atcgaattgg catcaaaatt acttttgtat tatctgtatt ttctatattc catctatata
      781 tggtttcttc tacttcattc ctctttgctg ctatatcacc tggtgatgct ataaatactt
      841 ttattagatt tatggttttc atttattgta cctttctctt tatgtctctt taaagtaatt
      901 tttgtataaa atttattgac ttttacacta taggacaact tttatctatt tagtacaaaa
      961 aggcccacta aactaattga ttagttagga gcctattaca ggtatatttt caactcgctt
     1021 ttaagcaaat tctactacca gctttttgcc taatgcccta gcgatatcat caagtttatc
     1081 gaaagatacg ttatcccctc tctcaattcg agcaatagta gattgagttg tatgtgcttt
     1141 ttgtgcaagc tccgcttgag ttaggcctgc ttcctcccgt gcatgataaa gagcaaccgc
     1201 agtttcgagt tttcttcctt cacggtcata tgcttctgca aaatctttgt ctttttgctt
     1261 ttcttcaatc aattttttaa tcatcatttg tgaaccactc ctctctaagt tcttttgcat
     1321 gtttaagttc acttattggt gttttctccg tttttttagt aaatccatgg gtaatgacat
     1381 accttccatt gaccacatgg aaatagatag ctctttggat gtttgatcct accttacttc
     1441 ttaattcgta caaattagtg tctaattttt tcacccattt caaccgttga gctactagca
     1501 tcccattctt ttctgtttct tctattgtac gtaaaagctt cgcactatct ttttttggta
     1561 atgtttccaa ccattctaaa aattcatcat gaccattagg tcttttaaat gtttcaaaag
     1621 atattttttc catacccaat tatatcatat att
//
LOCUS       DAXWLH010000289         1648 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXWLH010000289 DAXWLH010000000
VERSION     DAXWLH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.296"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..201)
                     /locus_tag="ACGE36_002816"
     CDS             complement(<1..201)
                     /locus_tag="ACGE36_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFF8385136.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIM"
     gene            724..1398
                     /locus_tag="ACGE36_002817"
                     /pseudo
     CDS             724..1398
                     /locus_tag="ACGE36_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1391..1552
                     /locus_tag="ACGE36_002818"
     CDS             1391..1552
                     /locus_tag="ACGE36_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385137.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          620 a          253 c          284 g          491 t
ORIGIN      
        1 cataataaaa aggtcaactt tttcaggtat ttgttcgatt aagtaatcga tttttgtttt
       61 taatcctcca atggaagtga cctctgattg gatagaagca gttttgtttt gccatccctc
      121 tttttggttt agtgtgctag taatataagc accttcccaa tcggcatatt cctctaatag
      181 aaacgtaatc gcagttttca ttttcaagct ctcctttact atctatgaca aggacaacag
      241 ttttaacata cagacattca ttaaatttgg atagcgacag cgaaaacaac aattcttaag
      301 ttttagacag agtatacaga cagtcaactt aattaaatac gaactaaata aaaatgacag
      361 gtaatcattt ttgtttacca gcattataac attttgcata catggatgac aaaagaacca
      421 attcccactt gcttgagtac aatcttaaaa gaaactggaa ggcaaaaagt aattatagaa
      481 acgaaaaata aatcatatcg ggcactcaga catgaaaaca accctagaaa tatataacta
      541 gtccaatgaa aaaaaagcga atgccgtaga aaacaaggat tctgcgtaca ttcatatctc
      601 attttgcgag catatctgct attactgtga ctaaatgtta atatggttaa aatgaccgtt
      661 atatttttta tataaatatc gtataattag tatgaatata gaagaaggaa agaaggtgca
      721 atcttggaac ctaaagacaa gtatttagat tacgctaggg tcaataaaga taaatttatt
      781 gaaacaatca tcaagggaaa aatccctgat gatgaaaaag atgcggtatt catggctgga
      841 agcccaggag caggtaaaac agaggttgca ctaggtttag cttaaaatta tgacaaccat
      901 gttgtgattg atgcggacta ttttagagca caatttccag aatacaatgg aaagaactca
      961 agtgtttttc agaaagcttc ttcttggtta gttgaacaat cattaaaata tgtactggaa
     1021 catggctatt ctttcatact agaggatact tttgctatcc ttagtgcaga gaaaaatatt
     1081 attcgagccc ttaaaaataa ctttcgcgtt acgattttct atgtttacca agatcccaaa
     1141 gttgcctggg atttcactcg aaaacgtgaa ctagctgaag gacgtcatgt gccaaaagaa
     1201 atgtttatca atgcgttttt taagtccaga gagaatattg aaaaagtcaa aaatagacat
     1261 cctgaagtag tccttcacat aatgattaag gattatcaaa acgacatttc tgaagtgcat
     1321 tatgcagcag ataatattca attagtacta ccaatacaat atacttcaaa agagttggag
     1381 gaggaattac atgactgaga aagaacaagt acaacaaatc gtgaagaagt ataataaaag
     1441 tatcgctgat ttatcagaaa acgcctcagc gaaagaattt aaaacagtaa tgaattacgt
     1501 agcagatgaa gcaaatcgta agcagcgaaa actagttggg ttggataaat aaatttttta
     1561 aaccatatcc ttgtaaagtt gagtttttta ttttaatgaa ttaagactat taaatagata
     1621 attattgtaa cttaaaaaac acagctaa
//
LOCUS       DAXWLH010000290         1626 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXWLH010000290 DAXWLH010000000
VERSION     DAXWLH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.297"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            2..400
                     /locus_tag="ACGE36_002819"
     CDS             2..400
                     /locus_tag="ACGE36_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8385138.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1078
                     /locus_tag="ACGE36_002820"
                     /pseudo
     CDS             384..1078
                     /locus_tag="ACGE36_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          616 a          234 c          323 g          453 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc agcgataacg ttaggtaaag agctactaga
      961 tcaagtatta gagatgccct cgattgataa agcagtgagg ggcttccaag aagattgggt
     1021 gcaaactttc caagcagaaa cgattggtga actaaaaaag gtcgctgatt acttctgaat
     1081 caatttctcc ataaaataaa cgatagcctt ctcgaaaact cgaggaggct atctgacgat
     1141 tcaatattca ttaatttaaa gtttgggtga ctattactaa ctattactta tttctcattt
     1201 aaacgatgta ttccctaacc tctaatacat ccttgtaact ctgaagttac gccagttttg
     1261 aataaatttc ataaaaacaa atgaaaagtg tcgcataaaa atatctttcg tgtttctcta
     1321 gtataacaga ataagaagaa caagtccaga agggatattt ttagtctttt ttaaaataac
     1381 ttttcagtcg agtttgagtt tatgatagtt gtttttgaag cgatacggca agtgaaaaaa
     1441 tagggaagca tgaaagaatc tacttaatgg cgcattgatt catgtgatag aaataaaaaa
     1501 cttttttaaa ttatatcctc atgattgtaa caaatccagt gatttgtttt ggtacaaata
     1561 aaaaaaggcc ccgatgaagt tgtggcaaaa gtggtttgcc tcaaaaataa gaaggaattt
     1621 ccaaaa
//
LOCUS       DAXWLH010000291         1611 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXWLH010000291 DAXWLH010000000
VERSION     DAXWLH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.298"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(144..443)
                     /locus_tag="ACGE36_002821"
     CDS             complement(144..443)
                     /locus_tag="ACGE36_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8385139.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            794..1426
                     /locus_tag="ACGE36_002822"
     CDS             794..1426
                     /locus_tag="ACGE36_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8385140.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          566 a          249 c          224 g          572 t
ORIGIN      
        1 attaaaggat aaaatacaat ataaagaaac atctgtttct ttatttagaa taaaaaaata
       61 cagactctgt tcaaataaat gaacctagcc tgcattatga gattgactat actttttaat
      121 tcattgagat gaataactcg tttttataaa tgcccaaaag taatcggtgc cgaagccatt
      181 cctcgaccta attctttttc aaattttcct attgttgata tctccatata aaacggtgat
      241 actggtttag acaattctcc cctgattgct aacggacgta aactacgttc caaattattt
      301 atgactttag gataatactg acctttttct aagcctgtct ttgcgtctat caaaagtata
      361 cgttcctttt ctttgatctc tggatttaaa acgagatcat ataattttag taccatgttt
      421 tctatttctt cacttttaga catcacgatt tcctcccaaa aatatatttc ttcccactac
      481 attctataaa caaagtatac gctacatcta gattttttta aatcattact ggattgttaa
      541 aacctttcaa tacactgtta tggcaacgat ttttatttta aaaacattct aaattaaatc
      601 agtaaaaatt tttattctaa gatttttatt agaattacgc ctaatttgtg tctctcaggg
      661 caattctaag aacgtttcaa gagatagcga cactatcgtt atcgaaaaag gcgaaaacct
      721 atctcatttt ctgctaatgt aagttggttt agcttttgtt aatatgactt tatgaaaatg
      781 gaggtaatta aatatgattt ttcctaaagt taatgaagca agcttgaata aaggatcctt
      841 ttttcatatc tatgaaaacg aagattttga actgtataat atcctttcac aaaaagaggt
      901 catcgaaaac caaataatct gccatccaat tatttacaaa tctattttca agtatgcatt
      961 ttttgcttcc aatgattttc aaagaatgga gattaccgac tgtatattcg aaaactgtga
     1021 tttttcaggc tgtatattga caaaaagcat gttgcatcgc gtgaaatttg tcaattgtaa
     1081 attaacaggt actaaattga tggaaagtta tattgggaac acgttattcg agaattgtaa
     1141 aatggactat gtaaatttat ctggttcaaa cataaaagaa agtacctttg agcactctgt
     1201 cttatcaagc gcagattttg tagactgttc cctcactaaa ataatgtttt ctactaatga
     1261 tttagaaaat gctagctttt ttgatactga tttaaaaaat atcgatttaa gtagcaactc
     1321 ctttgaaatg attgaagtac gctcggaaca tctaaaaaat tgtacgctta atcagttgca
     1381 agcacttggt tttgctagaa aatttttaca aataaaagta gtataatcaa aatgaatata
     1441 taagataaat ttttagcaat acaaatatcc ataaaaaaac attgcctatt ttaactcttc
     1501 atgtatactg actctatctt caaaaactga atagtaggag ttattatcat gaataacatt
     1561 ttaggaaaaa aaatagaact taaaaaaggg atattgggca ctattgtcgt t
//
LOCUS       DAXWLH010000292         1607 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXWLH010000292 DAXWLH010000000
VERSION     DAXWLH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.299"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            133..387
                     /locus_tag="ACGE36_002823"
     CDS             133..387
                     /locus_tag="ACGE36_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385141.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            457..702
                     /locus_tag="ACGE36_002824"
     CDS             457..702
                     /locus_tag="ACGE36_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8385142.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            778..1143
                     /locus_tag="ACGE36_002825"
     CDS             778..1143
                     /locus_tag="ACGE36_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFF8385143.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1204..>1607)
                     /locus_tag="ACGE36_002826"
     CDS             complement(1204..>1607)
                     /locus_tag="ACGE36_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385144.1"
                     /translation="TLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRC
                     MGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFM
                     SREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          446 a          293 c          288 g          580 t
ORIGIN      
        1 tatcagcaga aaactgttct gctttttcat ttttcattga tctattcttc ttttattttc
       61 ctagcatttc atttgtgatg tatgcaataa catgttatct tttattatat gaatcgttga
      121 ggagtccgtt acatgattac gttggtgaga gttttgtttt gggtgcctgc ggtcgcactg
      181 gtcgcaagta ttgtttatct tatgaattgg aacaaagaac ggttttattt agctattttg
      241 actcttccgg ctatctactt tatgtggaaa gtttttaatt acaactactt tgaacccgat
      301 agtgttttta tagaagaatt atcaggactg gttttgtctt tattgatcgt cattctttat
      361 ttgatacgtt taaataaaaa acattgagta ctattgattg catgtgtagc tttctatagt
      421 cattataact attctactat aatgaaaggt gattaaatga gtatcgaaga agctatttta
      481 ctggatactt tacgttgtcg tctgaatttc ttaagaactg gccgatcttt atctcaagta
      541 gctgtttcta gcgcgttaag tattccttta ttcaaatatc gtaagtacga agaaggagaa
      601 gaaattccaa gcatcattga attaatctat atcgctgact tttacgatat ttccgttgat
      661 tacctaattg gtcgctcaga aacagcaagc aaactgaagt gattttttcc agccttggtg
      721 ctggtttttt catttaacgt ctaaggttaa caatctatat ataaaggaga catttctatg
      781 cgattatggc atgaaaaatt gatttcaaag cttcctcgtc aacagctttt aggacaacat
      841 cgggaatgct gtgcattaag aggaaatggc tggggaaaga aacatgctac tgtggattat
      901 gtttttcact attctcctta tagattattc caatatcatc agcttgtcat ggatgaaatg
      961 gaaaaacgtg gttacagccc agcaccagag tggaaaaatc ctatgtatcg tggcaaatca
     1021 tgcgaagcat tttttgattt acctactgta gagcgcaacg tgccgattta tccggaacac
     1081 gatgaagttt atctagcaga atgtttagca aatttggagc aaaaaggaat ttatcttacc
     1141 taatataact agaaaagcct tctatctcct cttacttcat taaaaagctt taaccatctt
     1201 agatcaagct aaaatgtcgt aagttttagg gttatcttca tcctcacgtg caaaactaaa
     1261 agcgtactct agttttggtt catcgccaac ttccctagac ataaatgaac atctcctgac
     1321 gtggtttaaa agtaccgcat cttcgacgtc tgtatacgat gcacagcaca gattgcttct
     1381 tttgatcttt cttttgattt ttctgttgct tcttctacac cttcttcgat tactacatcg
     1441 atgcttacaa ttgcttctgc ctaatattcc catgcatcgt cgatttaagc ctctgtagtc
     1501 actgtgatcg tttccacatt ttaactcctc tgtcgtggct tcagttgatt ttgtgatcag
     1561 ttcagttgct aagccagagt cagaacaacc tgccattgct agcgtta
//
LOCUS       DAXWLH010000293         1601 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXWLH010000293 DAXWLH010000000
VERSION     DAXWLH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.300"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            1..1601
                     /locus_tag="ACGE36_002827"
                     /pseudo
     CDS             1..1601
                     /locus_tag="ACGE36_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          638 a          262 c          346 g          355 t
ORIGIN      
        1 aagaaaaaca agtagaaaaa cttgaaagac aagaagagat aaaggaaatg ggctataaca
       61 agtacggcgg actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac
      121 gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat
      181 tggaaacatt agaacagctg aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg
      241 aagtacaagc aatcattgac agcggacgag tgacgcaatg gaaaaaaatc cgaccattta
      301 ttttctgaaa ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga
      361 atctaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga
      421 agaggctgta ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga
      481 attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt
      541 cgaattgatc gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta
      601 taaattctat tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct
      661 aggggatggc gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca
      721 agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat
      781 gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat
      841 taaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca
      901 ttctcctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt
      961 tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc
     1021 cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt
     1081 cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc
     1141 tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta
     1201 ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc
     1261 gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact
     1321 tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga
     1381 aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt
     1441 tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac
     1501 aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac
     1561 taaagaattg caggcagaaa tgacttctta tattgtgtgc a
//
LOCUS       DAXWLH010000294         1590 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXWLH010000294 DAXWLH010000000
VERSION     DAXWLH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.301"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(451..>1590)
                     /locus_tag="ACGE36_002828"
     CDS             complement(451..>1590)
                     /locus_tag="ACGE36_002828"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8385145.1"
                     /translation="SHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSA
                     KDILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYH
                     AEHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYEC
                     DLACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIH
                     DIDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDL
                     AQMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIED
                     VCFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          447 a          334 c          248 g          561 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact
//
LOCUS       DAXWLH010000295         1578 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXWLH010000295 DAXWLH010000000
VERSION     DAXWLH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.302"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            66..1532
                     /locus_tag="ACGE36_002829"
     CDS             66..1532
                     /locus_tag="ACGE36_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFF8385146.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          438 a          291 c          332 g          517 t
ORIGIN      
        1 tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat
       61 agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt
      121 tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct
      181 attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag
      241 ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat
      301 ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg
      361 gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga
      421 ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt
      481 caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag
      541 gggcattatt agcaccacta atcgttggta caatcggtca agaatataac tatcatttag
      601 ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac
      661 gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta
      721 aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg
      781 cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga
      841 ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag
      901 aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat
      961 tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc
     1021 tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta
     1081 ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag
     1141 tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc
     1201 cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct
     1261 tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat
     1321 tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt
     1381 cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact
     1441 tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac
     1501 caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt
     1561 atttgcagca gttcctgt
//
LOCUS       DAXWLH010000296         1572 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXWLH010000296 DAXWLH010000000
VERSION     DAXWLH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.303"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            196..810
                     /locus_tag="ACGE36_002830"
     CDS             196..810
                     /locus_tag="ACGE36_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385147.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(834..1184)
                     /locus_tag="ACGE36_002831"
     CDS             complement(834..1184)
                     /locus_tag="ACGE36_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8385148.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1178..1441)
                     /locus_tag="ACGE36_002832"
     CDS             complement(1178..1441)
                     /locus_tag="ACGE36_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFF8385149.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          511 a          287 c          241 g          533 t
ORIGIN      
        1 ttttcttgat tgacatttat ttacaaaaaa tattgccatt taaaaagaaa agtgacacat
       61 actatttcct ccatcattag tttttgtcta tataaggtat accctaacta gacacgaccg
      121 cgacattgaa aaatctggtt ttctcgtgta tgattagggt gttttttaaa tagttgcaga
      181 aagtaggtaa aaattttgag agttgcatac atacgtgtct catccatcga tcaaaatgaa
      241 caacggcaga ttgaagaaat gaaaaaattc ggtgcagaga gaattttcat tgagaaacaa
      301 tctggcgcaa cgattactca tcgacttgtt tttcaagaag cactggattt cgtccgagat
      361 caagatattt ttattgtgga agctattgat cgacttggaa gaaattacga cgagatcatc
      421 gcttcagtca attacttgaa gaagaaaaat gtgcagctga tcatcacaag tctaccgatc
      481 atggccgaag caattggcaa tcctctcctg gataaattca ttaaagattt gatcattcaa
      541 attttggcca tgatcgctga acaagaacgt accgaatcta aacgacgtca ggcacaaggc
      601 attaagattg caaaagcgaa cggtgtttat aaaggacgtc ctaagctata cagtgccaat
      661 gcaaaagatc cccaacgtcg tcttgtttat aaaaacatcg ttgaggatct aaaaaaaggt
      721 gttgcgatcg ctaagattgc aaaagactac aatgttacga gacaaaccgt atatagaata
      781 aaaaaagaca gcatggtcaa tcacgaatag ttgatcatgc tgctgtttct attttatttg
      841 cttacttttt gaaaaactgt tctaactttc atcaataact cctcaactaa actatcatgc
      901 gctttttcca aaaaaataca ctctctggct tcataatcaa ttgtcgttag ttgatctaat
      961 aatacttttc ctgttgtctt catttcatat tctgagttta tatttacata tagcggatag
     1021 tttctagttg tactagaaat tggagcaact gtaacaacgt tactataatc cgcaataata
     1081 tcatgactta aaacaatata aggtctttta cctgtctgtt cgtgtccaga tcgaggtgct
     1141 gtattcaata atagaatatc gccttgtcta ggttttctca ccatttttca ttacctactg
     1201 cttcacctaa attttgaatc gtcacatgat ttctttcatc agtgtaattt tcaaataact
     1261 gttcaattgt taattcattt tctatattag ccgcttcgat aattagccgc ttctgaccgt
     1321 ttacttcttc ttgtattatc ttcactttgt cttctgtatg aattccaaga tattccataa
     1381 tatactttgg caatctaagg ccattactat ttccccattt ttttataggt aacatttcca
     1441 tagtagacac ctctttctgt atatacatag tataacatac ataagaaaaa acgtatatac
     1501 ttaattatcc ttttctatta aattagatta acattccatg aatccataaa ctatgttaac
     1561 atttatatga aa
//
LOCUS       DAXWLH010000297         1543 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXWLH010000297 DAXWLH010000000
VERSION     DAXWLH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.304"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(233..>1543)
                     /gene="cls"
                     /locus_tag="ACGE36_002833"
     CDS             complement(233..>1543)
                     /gene="cls"
                     /locus_tag="ACGE36_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8385150.1"
                     /translation="VGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGL
                     YPHPPTGQVDVKQLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHF
                     QYYIYRSDALGEEVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFF
                     FPLFVPYINPRINYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYG
                     EAVYTLQNRFLMDWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTY
                     LKMISLAKREILIQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSF
                     AAELIEYGARIETYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERM
                     ASRVRKAFFEDSKISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT          453 a          332 c          259 g          499 t
ORIGIN      
        1 aaaaaaacta tctcattaaa agtttctatg ctatactggc tttgaaatgt gtctctttta
       61 atagcatata ttagcttatt tcttgctttc cccaaaaaac aagagatacc ccaaagttaa
      121 tttcgctcat gcgagacgct ttctccattt ctgacgtact ctcagaaatg gccccgttag
      181 aaaaggacac gagatctcca tctgttgatg gttctcatgt cctttttatg ttttaaagga
      241 gcggcgaaat caatcttgct aatccttctt tcatcttgat gattattcct cggttttcat
      301 acatttcttt tgtgagatgc gtagaaattt tcgagtcttc aaaaaaagct tttcttacac
      361 gtgaagccat tcgttcatca taaaccagtg tattgacctc aaagtccaag cgaaatgaac
      421 gaacatcgat atttgctgag ccaactgaaa caatgccacc atcgataatc atcgtctttg
      481 catgaataaa tccattctca tatgtttcga tccttgctcc atactcgatt agctctgcag
      541 cgaaagaata cgttgcccag taaacgagaa gatgatctgg tttgttaggt atttggatat
      601 gaacttttac gcctgaaaga agagccaact tcagtgcttc atgaatcgat ccatccggta
      661 tgtaataagg cgtttggatc aatatctccc gtttggctaa cgaaatcatt ttcaaataag
      721 tcattttgat ttgttcatgt tcggtatctg gtccgcttgt cacgatttga acagctatat
      781 caccagtaga gtgaatggac ggaaaaaatt tgggagaata gcctacttct tttgtatgct
      841 gagaattcca gtccatcaaa aaacggtttt gcaaggtata caccgcttct ccgtaaatac
      901 gcaaatgatt gtctcgccaa taaccaaatt tctttaccaa tcctagatat tcatttccaa
      961 cattgaaccc acctgtatat ccgattttcc catcaataac tacgatttta cgatgattgc
     1021 gataattgat ccgaggatta atatacggaa caaatagagg aaagaaaaat gcgacttctc
     1081 cgcctgcttt tttcaaattc tgaaaaaagc tggaagaaac ttgtgtggaa ccccatgcat
     1141 caagaaggac tctgactttt acccctcgtc ttgccgcctc tgtcaatgca tctcgaactt
     1201 cttcacctaa tgcatcactg cgataaatat aatactggaa gtggatatgg tcttctgcct
     1261 gcttgatatc ttcaatcagc gcatcgaatt tttcacgacc atctgtaaat aaagtgattt
     1321 catttttagt cgtatagagc gtactttcaa ataccgtgag catatagatc aattgtttca
     1381 catctacttg tccagtaggc ggatgaggat aaagccctcg catcaatgcc tgtttgtctt
     1441 cttcaatttc caggttctta ccgattttcc cttggatctt caaatcaaag attttctctt
     1501 tggaaatccc tcttcctaaa aaaatatata aaataaatcc tac
//
LOCUS       DAXWLH010000298         1543 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXWLH010000298 DAXWLH010000000
VERSION     DAXWLH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.305"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            61..981
                     /locus_tag="ACGE36_002834"
     CDS             61..981
                     /locus_tag="ACGE36_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8385151.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            1135..1479
                     /locus_tag="ACGE36_002835"
     CDS             1135..1479
                     /locus_tag="ACGE36_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFF8385152.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          524 a          252 c          314 g          453 t
ORIGIN      
        1 aaactgttat tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt
       61 atgactgcgt atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga
      121 ttgaccgctg cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg
      181 atgaatggga tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata
      241 gacacaggaa catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa
      301 gtaggaaaac gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg
      361 atgctgcttc cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt
      421 gttttattag gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg
      481 gatatcatct ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg
      541 attctaaatg gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg
      601 tatacgatta tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat
      661 caaaaagtaa cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca
      721 aaacggatgg tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa
      781 gggaacatga tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt
      841 catgaaatcg acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg
      901 tttgcaaatg aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa
      961 aatcgttcac aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca
     1021 tctaataaga acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca
     1081 agcagctaaa aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc
     1141 gaagcaaacc aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct
     1201 gccttggaaa tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt
     1261 tatgatatag aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact
     1321 atgggctgtc cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg
     1381 ccagaagtaa cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag
     1441 atgagccgtt atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat
     1501 gagaagcaaa taggaaaaac aaaaaagcct tttatccttt cta
//
LOCUS       DAXWLH010000299         1535 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXWLH010000299 DAXWLH010000000
VERSION     DAXWLH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.306"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..222
                     /locus_tag="ACGE36_002836"
     CDS             <1..222
                     /locus_tag="ACGE36_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8385153.1"
                     /translation="LKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRV
                     AYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            506..1363
                     /locus_tag="ACGE36_002837"
     CDS             506..1363
                     /locus_tag="ACGE36_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8385154.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          560 a          213 c          311 g          451 t
ORIGIN      
        1 ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc atataagggt
       61 gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata tgcgggcaca
      121 acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt gttatgtgga
      181 acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt tgtcgttctt
      241 aagaaggacg gcttcttttt atcgaaaatc tgattcgtct acatgactaa agtcgcgagt
      301 attctctgct tctcgataaa ttaaataacg tcattacttt gaggaaagct ataaatcaat
      361 atatttcagc ttttccttat ttatgtacta ccttttactt attaattata aggtagtaca
      421 tatatatctt tttttggtag taactaaaaa taaatatgtt ataatagtat caaatgaata
      481 ttgctaaaag aaaagaggta ggaacgtggt agcgaagaaa tatgtaatcg gtaattttaa
      541 aggtggcgta ggtaaaagta cgtgcgcgca aatgttaggg tttgaatcag cagttaatga
      601 aggaaaaaga accttgataa ttgacttgga tatgcaagga aatacttctg atgtgatgaa
      661 cttaacgcat atgaacttca ctaacgaaga aggaggcgga gaaggtgaac cgattgttta
      721 cgaaaatact gtgactgacg tgttgataaa caatcttcca cctaaagagg ctgtctataa
      781 agtaatcaat aacctctata tacttccagc agacatgtcc tttgaaatgt atgatgattg
      841 gatcaaagac caatataatg attcattatc ccaattcaaa tatatgaaag aaaagctaga
      901 accattattt gatgagtttg acgtcatcta tttagatgtc ccaccttcta tttctgtata
      961 tagtaaatcc gcgatgtaca ttgcggattg ggcaatcgtc gtcttacaga cccaagtaaa
     1021 atcgatgaga aatgcgatgc aatatcttga gtatatggac ttttttgtga aagaatttga
     1081 tacaaattta tatgttgctg gtgttattcc tttcatgttg gaaagcggtg acgcagtaga
     1141 tcaggaaatg tataaacaag cccaagagat ttatggggaa catttgatta aaaatgttgt
     1201 actcaaaaat gcgcgcttaa aacgatacga cggatcaggt attacgatgg agaagacaaa
     1261 gaaaggtctc ttaaaacaat gggatcgcaa aagccacgaa ttatttatca gtattttgaa
     1321 agaattggaa gaacacgaga attggtatgc ggaggtggag taattggctg acatcaaagg
     1381 acttgatatt tttgggaaca acaaaggact cgccaaaata aaaaaagaag cagaagcaaa
     1441 gaaaaagcag caagcaatta caccatctga aaataagcaa ttagaagtgc accaagttca
     1501 agaaataaaa acttcctcca atgttgaaag acaga
//
LOCUS       DAXWLH010000300         1530 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXWLH010000300 DAXWLH010000000
VERSION     DAXWLH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.307"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(88..1218)
                     /locus_tag="ACGE36_002838"
     CDS             complement(88..1218)
                     /locus_tag="ACGE36_002838"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFF8385155.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1278..1505)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          431 a          310 c          243 g          546 t
ORIGIN      
        1 ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga ctttttggga
       61 cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga cttgctgcaa
      121 taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt ctgttgaagc
      181 aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt gtgcttcttt
      241 tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag taactaatcc
      301 taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat ctcctgctcg
      361 gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact cttctgcaat
      421 gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc agatatgtgt
      481 tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct ctgtacaaat
      541 atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt tagtttgatc
      601 agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt tttcataaaa
      661 agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag atagatcagg
      721 ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc gtaaaatcat
      781 cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca ctacttcttt
      841 cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt tatctactac
      901 ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc cagtttctgg
      961 aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa atccacgccg
     1021 aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca ctaatttttt
     1081 tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt cttgaacttt
     1141 ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat ctactgtaaa
     1201 tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt
//
LOCUS       DAXWLH010000301         1518 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXWLH010000301 DAXWLH010000000
VERSION     DAXWLH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.309"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..487
                     /locus_tag="ACGE36_002839"
     CDS             <1..487
                     /locus_tag="ACGE36_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8385156.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
     gene            complement(694..1218)
                     /locus_tag="ACGE36_002840"
                     /pseudo
     CDS             complement(694..1218)
                     /locus_tag="ACGE36_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1322..>1518
                     /locus_tag="ACGE36_002841"
     CDS             1322..>1518
                     /locus_tag="ACGE36_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8385157.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTV"
BASE COUNT          489 a          286 c          294 g          449 t
ORIGIN      
        1 tttaaaggaa gcgatcgatc aaatcaagac ggctgtcttg tatcctcata acggattacc
       61 gattttattg atgggtgtaa gcggttcagg aaaaaccttt ttggcagaaa aaatttatga
      121 atacgccaag gaacaacagg tgatagaatc aaatgctcca tttatcgcct ataattgtgc
      181 tcaatacttt aatttctcga ttaacaagat cgtagaaaat ttaacgatcc ttgatgcaat
      241 gaaggttttg agattggtac aaaaggccgt caaagaactc gaaaaactaa tggggatcga
      301 attttcaaac aatcaattgt tcttgcttta catgcactgc tgctcaatga tcgaacgtat
      361 tttacgcaaa gaaagtgtag atgaacaagc agatatcaaa gaatatattc aaaaagaggg
      421 acataattat gaaatatcaa aatacccggc ttacaaaacg aagtttgaaa gtactgtaat
      481 aaagtaagat ttaaataagc ataaacaagg taagaaccat aggggctgtg tcaagtccaa
      541 aatcttgtgt aaaaaggaaa tctcaaagta cgatgttttg ccttgttgag cctagctacg
      601 gtccaatatg ctggtaagaa gtgtcaagag caaaaagcga agcgtctctt gatacttctc
      661 accagtatat tatactattg gcggcttaat gtgctaaatc tcgttacgat caaattcaag
      721 atattttcta ggtgcagaag tccattcgtc atccttttcc atcaataaag caccgatgat
      781 acgaataaca gaatcacaat ttgggaaaat tcggacaaca cgttcacgac ggcgtacttc
      841 ttcatttagt ctttcaagaa cattcgttgt tctcaatcgt tgacggatat tttctggtaa
      901 aaccattact tgcatggcat cttcgaagcc ctcatccaat attttacagg cagttgcgac
      961 tttcttgtct tccccatatt tatccataat aagttgttta cgttctccag ccagctcgat
     1021 gcttgccgtt ttgaagatgg ctttaaagtc ttctttgata tcgcttgaga ttttcttgga
     1081 gaatttgtcg gaaatgttgc gaaggagatg tgcttggcat ctctgctagc tcgcacaaag
     1141 aaggtattct ttcacagctt ttgtgagccc tatatgcgca tccgagatga ccatctttac
     1201 tcctgaaaga ccgcgttctt tcaatccgag agtgtaaaat attttgtgta aatagaaaaa
     1261 aggaagtccc ttcagtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
     1321 tatgaacgat tttactacag aaattctaaa gactctagcg aataaaggcg atttgaatga
     1381 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
     1441 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
     1501 ctcctatgac cgtacggt
//
LOCUS       DAXWLH010000302         1508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXWLH010000302 DAXWLH010000000
VERSION     DAXWLH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.310"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            25..1167
                     /locus_tag="ACGE36_002842"
     CDS             25..1167
                     /locus_tag="ACGE36_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8385158.1"
                     /translation="MAKFEQYKKKNGDKAWKFQAYLGINPETGKPVKTTRRNFKTQRE
                     AKLALARLQSEYEDNLLTKEKPKTYKDVYDLWMTEYKRTVRGSTLLKTERIFKNHVLE
                     ELGDIYISEITPIKIQKLMDKWANKYDTAPKMMNYTGLVFKYAVRFGMIESNPTDAIR
                     KPKRRKKATVEEPFYDKKQLKLFLDELYNQPNLKIQAFFRLLAMTGMRKQEAGALEWR
                     DIDFKAKTVNIYKAVTRTANGLEIDTTKTVGSSRIISIDQGTLDKLLEWKKVALPPSD
                     EWLIFGHSSAKKPHDIMSLDTSRKWLLSIQDKMDKKQKKKLPRITVHGFRHTQASLLI
                     EMGASLKEVQFRLGHEDIQTTMNTYAHVSKLAKEQLADKFNKFIDF"
     gene            complement(1294..>1508)
                     /locus_tag="ACGE36_002843"
     CDS             complement(1294..>1508)
                     /locus_tag="ACGE36_002843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385159.1"
                     /translation="NHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLN
                     VFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT          594 a          233 c          265 g          416 t
ORIGIN      
        1 taccagaaaa gtgagttgat tcaattggct aaatttgaac aatataagaa aaagaacggg
       61 gataaagctt ggaagttcca agcatattta ggaatcaacc ccgaaacagg aaagcctgtt
      121 aaaactactc ggcgaaattt taaaactcaa cgtgaagcaa aattagcact cgcaagattg
      181 caaagtgaat acgaggacaa tttattaaca aaagaaaaac caaaaacata taaagacgta
      241 tatgatttat ggatgactga atacaaaaga acagtacgag gatctacatt gttaaaaaca
      301 gaaagaattt ttaaaaatca tgtattagaa gaactcggcg acatatatat ttctgaaatc
      361 acgcctatca aaattcaaaa attaatggat aaatgggcaa ataaatatga tacagctcct
      421 aaaatgatga attacacagg actagttttt aaatacgccg ttcgatttgg aatgattgag
      481 tccaatccta cagatgccat acgcaaacca aagagaagga aaaaagcaac tgttgaagaa
      541 ccattctatg ataaaaaaca attgaaattg tttcttgatg aactatataa tcaaccaaac
      601 ctaaagattc aagctttttt tagattacta gctatgactg gtatgcgaaa acaagaagca
      661 ggcgctcttg agtggagaga tatagatttc aaggctaaaa cagtcaatat ctataaagcc
      721 gttactagaa ctgcaaatgg actagaaatt gacacaacta aaacggttgg gtctagccga
      781 ataatttcaa tcgatcaagg tactttagat aagcttcttg aatggaaaaa agttgccctt
      841 cctccatctg atgaatggct catttttgga cattcaagtg caaaaaaacc acatgatata
      901 atgagtcttg atacctctcg aaagtggctt ttgagtatac aagacaaaat ggacaagaag
      961 caaaagaaaa aactacctag aattactgta catggattta gacatactca agcaagcttg
     1021 ttgatcgaaa tgggagcatc gcttaaagaa gtacagtttc gtttaggtca cgaggatatt
     1081 caaactacta tgaacacgta cgctcatgta tcaaaacttg ctaaagaaca attagcagat
     1141 aagttcaata aatttataga tttctagcta tgtggcattc aaaatggcat tcaattaaat
     1201 ttgagcattg atataatagt atttaagcaa ctcccgccgt ctccatatat gtatggttga
     1261 gacagataaa catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga
     1321 gagaaaagta gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa
     1381 acatttagaa atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac
     1441 ttttgttcgt acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt
     1501 atggttta
//
LOCUS       DAXWLH010000303         1471 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXWLH010000303 DAXWLH010000000
VERSION     DAXWLH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.311"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..479)
                     /locus_tag="ACGE36_002844"
     CDS             complement(<1..479)
                     /locus_tag="ACGE36_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFF8385160.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVA"
     gene            779..>1471
                     /locus_tag="ACGE36_002845"
     CDS             779..>1471
                     /locus_tag="ACGE36_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFF8385161.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKRKRIMVPLPV"
BASE COUNT          453 a          244 c          258 g          516 t
ORIGIN      
        1 atagcgacat acttatcatt taaggctctg gatttaaagg catttacttc ttcagtcaga
       61 actttagtca tgttggacat ggtttgtgga gtatagtgat gaccgtacat tttttcgatc
      121 aaatcagcaa tttcagacat cgtaacacct ttttcgaata aatggataat agtggtttcc
      181 aatgtatcgt ttgttctttt gtaggctggt aaagtttgtt gtttaaactc accattacga
      241 tctctaggta tttccaatgt taattcacca tattcggttt tgattgatcg aaagtaagaa
      301 ccgtttctcg aattacctga attaaaacca gtgcgatcat atttttcgta atctaaaaaa
      361 gccgttaatt cagtccgtag gagtgtgttt atcgcttttt ctaagtgcga acggaataat
      421 tcatttaaat cgcctttagt gactagagtt tgcacaattt ctgtagtaaa atcattcata
      481 gggaagtcct cttttctgtg aattggttgt cgttaacttt attctacaga agcgacttcc
      541 ttttttgtat ggattttttc atttacacaa aatattttac actctctaat aattacacac
      601 acacaatttt ttaaggtctt aaaaccatca tatatgcaaa agaagctaac ctttattact
      661 aaattagacc cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt
      721 aaatcaacaa aaaaacgcat atagtagtct caaaactccc aagaaatgag gactacatat
      781 gcgttcatca aaatatactg aacattttga cttagccaat cctgttacta aagtcgatga
      841 tatccccgat tatgaaatgt acagtcaaac aatcgatagc ttaaataagc gttttggcaa
      901 tcgtgtttta aaaggcatag aaatcggtta catcgcttct gaaaaggatc ggatcatcga
      961 ctatttagcc gataaagatt acgatttaaa attactcagt gtgcatcata atggtcaatt
     1021 tgattattta gatgacgaag tcaaagatat ggatcctgct attgtgattc ctcagtattt
     1081 tgcacaactt tcagaagcac tagttgttat agaagcagat gtctttgctc attttgacta
     1141 tggttttcgg gtatttggtc tatccgttgc tgaatttaaa caatatgaag cgcagttttt
     1201 accgattcta gatcaagtaa tcaaaaataa attggcattc gaattaaatg ctaagagcgc
     1261 ttacttgtat gacaatttag cgttatatga atatgtcatc gatttatatc tttcacgtgg
     1321 tggtaccttg tttagtgttg gatcagatgg gcattacctt gaacatttcc gtttccattt
     1381 cgatgatctt tttgctctgt taaaagcaaa aggtgtgacg gaattagcga tttatcaaaa
     1441 aagaaagcga attatggttc cattaccagt c
//
LOCUS       DAXWLH010000304         1460 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXWLH010000304 DAXWLH010000000
VERSION     DAXWLH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.312"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(395..1405)
                     /locus_tag="ACGE36_002846"
     CDS             complement(395..1405)
                     /locus_tag="ACGE36_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFF8385162.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
BASE COUNT          477 a          278 c          197 g          508 t
ORIGIN      
        1 atgtgattta ttactgctac tatccaaccg tatgacctcc ctcaactcac atctatagat
       61 gactttctgg tttaattaga actacctaaa ttcctctgta tcgtgtgagt cctcgtagta
      121 aaaatttttt tgaatatgtg aatagctaag atcttttcaa ataaagatat ccatgatcaa
      181 ctatatattt cttcaatctc atcaatcaat attggcagtc agaatccagt tcacacatct
      241 tgatacacaa ttttgtttta tagaaattct tttagttata gaaaatatct attcggcgag
      301 aaagttcgtc acgtttgaaa gtagaatttc ctaaaatacc taacaaagct aaaaaaatcg
      361 tatgaattac ccatcttcaa tattcctttc aaaattaact caaataatac actgtatacc
      421 attcaacgaa ttttttaatg ccctcatcca aggttatatt cggtttaaag ttgatttttt
      481 cctctaaatc ttcaatatcg gcataagttc taacgacatc acccggctgc atgtccaaaa
      541 aaatcttgtt cgctttaaca cctaaatact tttcaattgt ttcaacaaac tccaacaatg
      601 caaccggctt attatttcca atattgtata tcttatatgg tgcccaactt tcactactca
      661 aatcatcgat gtctgtccag tcggagttgg gttcaggtac tttattgatt aatctagtga
      721 ttccctctac gatatcatcg atgtaagtga aatctctttc catctttcca tggttaaaaa
      781 tcttaatttc ttttccagat aaaatattct tggtaaaaga gaaataggcc atatctggtc
      841 gcccaaatgg accatagaca gtgaaaaatc tcagcccagt tgttggtatt ttataaaggt
      901 ggctataagt atgagccatt aattcattcg attttttggt agccgcataa aggctaactg
      961 gatgatccac cgaatcttct gttgaaaacg gtactttcgt gttcccacca tatactgaac
     1021 tagatgatgc atataataga tgctttacag ggaagtttcg gcaggcttca agaatattaa
     1081 gaaaaccgat aatattagaa tcaatataag cataagggtt ttcaatagaa tatctaacac
     1141 ctgcttgagc agctaaatga attactatgt caaaaccttc actttcaaat agagaatcta
     1201 atttttcttt ctctaataga tccattctga taaatttaaa attattataa tcgtacagta
     1261 tcgttaatcg atcttctttt aattggacat cataataatc attgagatta tcaatcgcta
     1321 caatttgatt ttcagtttcc ttgagtaatt ttctagacaa atgaaaacca ataaatcctg
     1381 ctccacctgt tattaatatt ttcattttta ttcatccacc tttttctgaa aggaaccaat
     1441 agggttaaac ccaaaattgc
//
LOCUS       DAXWLH010000305         1459 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXWLH010000305 DAXWLH010000000
VERSION     DAXWLH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.313"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            74..484
                     /locus_tag="ACGE36_002847"
     CDS             74..484
                     /locus_tag="ACGE36_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8385163.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            498..854
                     /locus_tag="ACGE36_002848"
     CDS             498..854
                     /locus_tag="ACGE36_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8385164.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          538 a          211 c          271 g          439 t
ORIGIN      
        1 tttattagct aataattggt attttaaaag aaattactgt agaattggat tataattaaa
       61 aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc
      121 aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc
      181 gttaacagaa agtgagatca aaaagatctt ggcagcgact gaaggaggag ttcaagatat
      241 tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt
      301 aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat
      361 tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg
      421 tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc
      481 atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc
      541 tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag
      601 aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag
      661 aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg
      721 taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt
      781 tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta
      841 tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta
      901 acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac
      961 tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta
     1021 aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca
     1081 aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac
     1141 atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa
     1201 aaacttcaaa aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt
     1261 aagatttcga aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc
     1321 tctgatatga tataaaagtt gctacggcag ccaaaaacga atcggggaac actcctgatg
     1381 aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt
     1441 caattcaaga caacaaaca
//
LOCUS       DAXWLH010000306         1455 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXWLH010000306 DAXWLH010000000
VERSION     DAXWLH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.314"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            109..1308
                     /locus_tag="ACGE36_002849"
     CDS             109..1308
                     /locus_tag="ACGE36_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8385165.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT          407 a          248 c          299 g          501 t
ORIGIN      
        1 gtaatctacc ctaatttcca tagttcttca ccacttttaa aaatagtaaa acccctacaa
       61 gtatgctata gtaaattgct aaagaaaaac caataaggtg ggagatgtgt gcgcgatcaa
      121 acaagcggaa aaatgattcg tttttattat acaagtttgc attttcttta ttggatgatg
      181 ttttgttctg tctatgggtt tgcgacattg tttttgattt atttcaagat tgatccagct
      241 aaaatcggag ggatcttagc attggtcaat attgtttcaa cggtgcttca gccttttgtc
      301 agtcaagtga tcatcaagaa aagaaagatc ccactgatca ccgtgctgaa gacgatgata
      361 gcaggattag gagtgattct cttagctggg gtattgattc cgaatttgcc gatcattact
      421 tatatcttgg gtgggatttt agtagtttct atgcaatcat ttctcaatgc gctggcattt
      481 gagtattcaa ataccggcta caggatcaac tttggctttt caagagctgg tggttcattt
      541 tcttatgctg taacttcttt tttactggga cagctgttag cgatttattc tgccgcgatt
      601 ttgccaatct tggttatagt tgaagcagtg atatttttcg ttattttgca tctgctgccg
      661 gatgtaaaaa atcaagaagt ttttcctatg acaaaagaac aggatcgctc agtttctttg
      721 agaaaaaaat atcctttcct gattttgtta tttttaggtt tcagttttct ttttactttc
      781 cacaccatga tcaacagctt tttggcacag atctttgaaa atatccacgg cggcagtaaa
      841 gaagttggca tcgcattgat gatcgccgct ttatgcgaat taccgggaat gatctttttt
      901 gaacagcttg tcaaaaagaa atccagcaaa ttttggctgt ctgttgccag tatttcattt
      961 gctgtcagag gattcatgat gctgctggcc agttcgatcc tgatgatgga aatgacgcat
     1021 ctcctacaag ccttcagttt cgctttgttt attccagctt cggcttattt gttcaatcaa
     1081 tttctggcgg atgatgatcg tgtatttgga caaacgatga tcatcatcgc cacaactctt
     1141 ggtggggtga taggaaatgt cttgggcggc agtctgctgc agcatttcgg tgtatgggag
     1201 atgctgattt tcggtactgg ttttgcggtg ctaggtgctt tattaactat tttgggcatt
     1261 cgaaaaatac cgaagacagg aaacgatagt gattctcttg ttgagtagca taaaaatact
     1321 tagatactag ttttatataa ataacatata gtgattattg gcgtaattac aaaaaacgag
     1381 aataacttaa gtaagcattt ctaaataatt gagaaacctc atctaaaaag ataagaaaat
     1441 atccctaata aaact
//
LOCUS       DAXWLH010000307         1435 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXWLH010000307 DAXWLH010000000
VERSION     DAXWLH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.315"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(59..131)
                     /locus_tag="ACGE36_002850"
     tRNA            complement(59..131)
                     /locus_tag="ACGE36_002850"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Gln,seq:ctg)
     gene            complement(177..605)
                     /locus_tag="ACGE36_002851"
     CDS             complement(177..605)
                     /locus_tag="ACGE36_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385166.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(665..1423)
                     /locus_tag="ACGE36_002852"
     CDS             complement(665..1423)
                     /locus_tag="ACGE36_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8385167.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          409 a          277 c          273 g          476 t
ORIGIN      
        1 cccctgcaaa aagattgttc ttttacaggg ggttcatttt ttcagcaagc acatcaaatt
       61 gggatggcag ggttcgaacc tacaattgct tgattcagag tcaagtgcct taccagtttg
      121 gctacatccc atttattcaa caaaataatc atagcacttt cctaaaaaaa cgcaagttat
      181 ttttcttgat cggatagttg ttgctttact cgtaaaaggc ggcgtactag tttcttttct
      241 aatccagcag gaagggcaat cagttctgct actgacaaga cggtgtccaa taactgatat
      301 tctttgacag gttcattcat aatatgatag atgtttgcta aacgttcaat atattcaaag
      361 cgagtatact ctgttcgagc aattagaaat ttcaataaat cggtggcttt ctcgtactct
      421 tgatctgcgt aataaacaag agattgacga tacaatttga tttccagttc attgagggta
      481 gcaggtctgc gacgctcgtt ccactcatta atagagactt taccttgaac atttcccttg
      541 cagtcacgga caaaaataat cggaatttca tttggatcca tttttttatg tgtgaatttt
      601 tccattatct acgctccctt caaaggtttg gtatcttgag tataagggat ttttgtttga
      661 attactagct tttctccaaa gttagcatct gtccacccat tgcagtgaaa atcgccgaaa
      721 aatcgttgat cggtactgct cgatttttta caccataggg atcgttgaca taaatatttt
      781 ctttgtcata tccagtgact acacaagcat gaacaagagg agtgactctt acttctccat
      841 tatttgtggt ccaagtcata aaatcagttt catctgggac ttcaaaattg atagtcacag
      901 aagtccagac aggatttcct ttagatagtg cttttttgat atcatcgagt tgagcatcgg
      961 tttttggaaa agtgtgataa tccgtttgga cgatttgctg agcaagggag gcaactgggg
     1021 gaacatagac gaccatagcc cagtctccgc cactgatatc accaacaaat ccttcattag
     1081 gatcgccatg agtcccatct gcattgaaaa cagggacgta atccaattgc tcagctaact
     1141 ggtttttagt tgtttcataa ccataaaaag ataaaagcat agaaagcgaa gtgacttcac
     1201 agccattttc taaagaagga gaatcaaact ggctctctaa tggaacatct agtaaaactt
     1261 ctgctttctc ttttgtcttt ttcgtttttt gtgttgaact tttttgtgca gtgttcattg
     1321 tatgttctgg agaatggata tgtaggtaat aacttgtacc gcttaataaa agagcagctg
     1381 tgccccctag aaacaatagt tttttgaacg tttttttctt catttgaaat ctccc
//
LOCUS       DAXWLH010000308         1418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXWLH010000308 DAXWLH010000000
VERSION     DAXWLH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.316"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..341
                     /locus_tag="ACGE36_002853"
     CDS             <1..341
                     /locus_tag="ACGE36_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385168.1"
                     /translation="STNEPTTVVDNNQELTPLDRNGIAIGNPTNDLTVTENYLIVSPL
                     LARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWI
                     ATYSGTLTRK"
     gene            351..554
                     /locus_tag="ACGE36_002854"
     CDS             351..554
                     /locus_tag="ACGE36_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385169.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            765..1349
                     /locus_tag="ACGE36_002855"
     CDS             765..1349
                     /locus_tag="ACGE36_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385170.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          507 a          208 c          267 g          436 t
ORIGIN      
        1 atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact cctttagata
       61 ggaacggaat agccattggt aacccaacta acgacttaac tgttactgaa aattatttaa
      121 tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca ggaacgatga
      181 tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg aaagaaaaaa
      241 gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca gctggaaaca
      301 attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt atgccagaat
      361 ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt aatttaccga
      421 aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa attcaaaaat
      481 caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc
      541 aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc gacattcttt ttttctaatt
      601 gaaagtgttc ataaaatcac ccatttctgg acactttctt tttttgcttt atttttcaaa
      661 aaaacaattc atttcatgtt cataaaccca taacaatatc tatgttcagt taaactatat
      721 ggataaagtt atgttacaat aaaattaatg aaggagtgat ttttttgaaa ataggatacg
      781 cccgggtatc tactggttta caaaatttgg atttacaaga agacagccta aataaatttg
      841 gttgtgaaaa aatttttact gatcatatga gtggggcaaa aagtaatcga ccaggattag
      901 aaatggccat tgattttgtt cgctctggcg atacacttgt cgtttggcgc ttagatcgtt
      961 taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg actgaatgag cgtggtgtga
     1021 gttttcatag tattcaagaa aatattacga tggacaaatc aagttctaca ggacagctca
     1081 tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa tttgatcctg gaacgatccg
     1141 cagcaggacg agcagctgct cgtgcaagag gacggtttgg tggtcgtcct gaaaaattgt
     1201 ctgaacaaga tttggaatta ctgaagactt tagtggatag cggtacacct attaagacta
     1261 ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg ttatttggca aaaatggagg
     1321 agaaaaatgg aaagacaaat tctcattagt ttttgtcatt taatgctgaa aatttttgcc
     1381 atttatctag aaaagtgaca atagtatgag aatttttt
//
LOCUS       DAXWLH010000309         1418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXWLH010000309 DAXWLH010000000
VERSION     DAXWLH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.317"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            38..1339
                     /locus_tag="ACGE36_002856"
     CDS             38..1339
                     /locus_tag="ACGE36_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8385171.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          462 a          285 c          260 g          411 t
ORIGIN      
        1 attgaatcac cacaacacaa tcaaaggagc gttctgtatg cctgatcttc atcatatcaa
       61 actactactt ggaatcaaag ataaaaacat tttcatcaca aatgtggaat cgaagagcat
      121 taagaatatc aagagtcttg ttgtttctgc gacgttaagt aaagaaattc gtcgctgtcc
      181 tttgtgtaaa cagatgaatc atgaaggcat gatcgttaag aacggaaaga aaaaatcgct
      241 cattcaactt aataaatgtg cgaaccaact gacttatctt gctttagcga aacaacgcta
      301 ccactgtcgt ggctgccaca cctattttac ggcgaatacc tacattgtcg atagaaactg
      361 ctttatcgct aaacaagtgc gctataaaat tttagaagag ctaactgaaa aacaagcaat
      421 gacgacaatt gctaagcatt gcggcgtttc ttggtcgacc gtttctcgga cactcgcttc
      481 tctgatcccc atgacaaaag taaaaagaaa ttggttaccg cgttgtttat taatggatga
      541 atttcgttca ctgaaaaatc aagtcggtcc ctactccttt tcttgtatgg atggcgatac
      601 tggaaaatta ctggatattc ttccttcacg gaagaaaaag gctctcgttt cttattttat
      661 gcaatttgaa cgacgcgcac gattgaaggt aaaggtcatc gtgacggaca tgaatgtttc
      721 ttatgcctct ctgataaagg aatgttttcc aaaggcaaaa ctcgttgtgg atcgttttca
      781 cattgtcaaa catctcattc gtaaatttga ggatattcgc gtacgaatca tgaaaagctt
      841 tgatcgaaac gatccaacac aagccaaata ttatcgacaa ttaaaggcgt tgtctcggct
      901 tctgatcaaa cgacaggaca agcttgttta tgacaaatgg accaaatggc gcaattttgg
      961 ttgggcgtat ctcaccgaaa gtgaagtggt ggatcgtctg ctttcgatct ctgacgaatt
     1021 acgaatcgcc tatttttact atcaagaaat tcttcaagcg tttcacgaca aagaggcgga
     1081 cactttcttt aaacttgtaa gaacgatgcc caattccgtt ccaaaagaac tgcattctat
     1141 aaaaaaagct tttataaact atgaatccgg cattcgttta gccctcgaat tgccttattc
     1201 aaatgccaaa atagaaaatc tgcataccca catcaaagca ttgaaacgag tcgcttatgg
     1261 ctttcgaagc tttcggaaaa tgaagaccag aattttctta ctaaataatc tgattaccta
     1321 tgaatcaaaa aacatctaaa gtagaaaagg aattctacct tagatgttct tatctcactc
     1381 tatcagtcct atttgacaaa gagccttgat aaagaagt
//
LOCUS       DAXWLH010000310         1417 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXWLH010000310 DAXWLH010000000
VERSION     DAXWLH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.318"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(150..1337)
                     /locus_tag="ACGE36_002857"
     CDS             complement(150..1337)
                     /locus_tag="ACGE36_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFF8385172.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAXWLH010000311         1416 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXWLH010000311 DAXWLH010000000
VERSION     DAXWLH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.319"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..228)
                     /locus_tag="ACGE36_002858"
     CDS             complement(<1..228)
                     /locus_tag="ACGE36_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFF8385173.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVY"
     gene            complement(244..447)
                     /locus_tag="ACGE36_002859"
     CDS             complement(244..447)
                     /locus_tag="ACGE36_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385174.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(462..1208)
                     /locus_tag="ACGE36_002860"
     CDS             complement(462..1208)
                     /locus_tag="ACGE36_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8385175.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT          386 a          265 c          236 g          529 t
ORIGIN      
        1 atagacaaat ccatttttgt ttttctcgat atcgataata tttctatatc ttgggttctt
       61 taaaactcta gaagtaaacc aatcatattt tctgttaatc cgttctagca cttctggcaa
      121 cgtcatccat ctaccagtat catcagcttt ttttaactcc tcataatcca cttgggagat
      181 aattacatag ccttctggaa ttgggatttt cgcttctaga tattgcatta gctgttcctc
      241 cctttacaat tcgtacatag taataatcga atctatgatt ctatttgctt ctgcagaagt
      301 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
      361 catggtgtaa gttaagaact tcgaactttc gacatattct ttgatttttt ctctatctcg
      421 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
      481 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
      541 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg aatctcactt
      601 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
      661 actttatgtt gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
      721 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
      781 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
      841 aaatctgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
      901 gtttttctga ttgttggaag gacttcttcc attacccagg cctcaaattt ttcagcgctt
      961 ggtaagttcg atttaataat caagcggtaa acatctgatt ctggaataac tttgaatttt
     1021 tgacgacgac ctaacgaatc gttaccccat gtttcaattg attttttaca atgatcattg
     1081 gtggctttac tgggattttt gtatcccaaa gttttggcaa catcgtttgc tacgaaatac
     1141 ggaatgtcat tttctagaaa cgttcttact tcatgttgtt caaaactaaa aatttgtggt
     1201 gtgttcattt ttgttcattc ctttctttga tataattttc ttatcagtaa gtggtctact
     1261 gaaataatgc ataaggtggt gaataagtat gaataaaaaa actgatgaaa tcgctgcgga
     1321 tttagttgtt gcatggctaa atcacgaatc acaagtatct actaaaggcg aatcaatttc
     1381 gccaaaagaa attgctcaag cttatttaga tattca
//
LOCUS       DAXWLH010000312         1414 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXWLH010000312 DAXWLH010000000
VERSION     DAXWLH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.320"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            3..1184
                     /locus_tag="ACGE36_002861"
     CDS             3..1184
                     /locus_tag="ACGE36_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385176.1"
                     /translation="MITMTKKIEVYVDGSQEERDSFFGFFWKNSREYVKVLNMVYRQL
                     MFEELIKDAIKEMDQGYVEREKSIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNK
                     EAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFVGIVTGQRTP
                     RVYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGSKPRKARDSALTLGYI
                     MDGKYKLNQSNLIFEGNKLFFNLTISFEKDYHKLEFKEGSELTIKFTDLCSGECLVNN
                     KSVIDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNF
                     VKTYNNQCSAKIVQTAIRNKAQIICISEPDKSYGEWAYYQLKEQIINKGKRYGIEVKS
                     V"
BASE COUNT          570 a          147 c          294 g          403 t
ORIGIN      
        1 caataataac tatgactaaa aaaatagaag tatatgttga tggatcacaa gaggaaagag
       61 attctttttt tgggtttttt tggaaaaata gtagagaata tgtaaaagtg ctgaatatgg
      121 tatatagaca gttgatgttt gaagagctga taaaagatgc aatcaaagaa atggatcaag
      181 gctatgttga gagagaaaaa agtattcaaa ataggattga aaagttagcg tatactgatc
      241 caatagatca aaaaaaactt gataaagcaa aagctgaatt atctaaattg agaaatgaaa
      301 aaaataaaga agcgagagaa atattaacgc aagcaatagg attgaagagt caaacgagaa
      361 cacgagatgt agcaaaaaaa ttagactttg attttggaga ttttgttgat tcagcaaata
      421 tgaaagctag taaggatttt ggcaatgact ttgttggaat tgttactggt caaagaacac
      481 ctagagtcta tagaaataaa agtaaatatg gattgttacc gttcagaggt cgggatatta
      541 agattcataa agctgacaag ttttatatct ctttacctaa aggatttagg tttaaagtaa
      601 atttaggtag caaaccgaga aaagcgagag atagcgcttt aactttgggg tatattatgg
      661 acggtaaata caagttaaat caaagtaacc taatttttga agggaataaa ttgtttttta
      721 atctaacaat ttcttttgaa aaagattatc ataagctaga atttaaagaa ggttctgagt
      781 taaccataaa gtttaccgat ttgtgtagcg gtgaatgttt agttaataat aaatctgtga
      841 ttgattttgg tgacagcaaa tatataagta acttcaaaaa taaaattaaa gcaatgaaat
      901 caagagagca gagccgagga gttttttcta aaggcggtca tggtcgaaaa agaaaattag
      961 atcaagatag atgggaagcc ataaagaaaa gagaaagtaa ttttgttaag acttataata
     1021 atcaatgttc agcaaaaatt gttcaaacag ctattcgcaa taaggcacaa ataatttgta
     1081 tttctgaacc agataaaagt tatggggaat gggcttatta tcagttgaaa gaacaaataa
     1141 taaataaagg aaaaagatac ggaatagaag ttaaaagtgt gtaaaataaa aaatcgttga
     1201 tttggcaacg aaatagtgag ggggcactca ctcactgaag ggtcaactcc gtcatattgt
     1261 gtgaccagac ggaaggacga atgttgttga ggttttggag ctatataaaa cgacatggtt
     1321 ctcaaatgat aaagccattc tttttcaatt aatgattgtt ttggagctat ataaaacgac
     1381 atggttctca aatgatacga aagaagagta tatc
//
LOCUS       DAXWLH010000313         1413 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXWLH010000313 DAXWLH010000000
VERSION     DAXWLH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.321"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            88..1380
                     /locus_tag="ACGE36_002862"
     CDS             88..1380
                     /locus_tag="ACGE36_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFF8385177.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          459 a          288 c          256 g          410 t
ORIGIN      
        1 cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg
       61 cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta
      121 gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa
      181 gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac
      241 tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta
      301 atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc
      361 aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc
      421 agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag
      481 atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat
      541 cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa
      601 tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc
      661 ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt
      721 tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta
      781 tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc
      841 caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa
      901 agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt
      961 caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct
     1021 catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta
     1081 ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt
     1141 gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg
     1201 ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca
     1261 cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc
     1321 ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa
     1381 aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DAXWLH010000314         1409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXWLH010000314 DAXWLH010000000
VERSION     DAXWLH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.322"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1359
                     /locus_tag="ACGE36_002863"
     CDS             <1..1359
                     /locus_tag="ACGE36_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFF8385178.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DAXWLH010000315         1366 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXWLH010000315 DAXWLH010000000
VERSION     DAXWLH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.323"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            80..>1366
                     /locus_tag="ACGE36_002864"
     CDS             80..>1366
                     /locus_tag="ACGE36_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFF8385179.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atcaat
//
LOCUS       DAXWLH010000316         1351 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXWLH010000316 DAXWLH010000000
VERSION     DAXWLH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.324"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            74..697
                     /locus_tag="ACGE36_002865"
     CDS             74..697
                     /locus_tag="ACGE36_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385180.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            complement(694..>1351)
                     /locus_tag="ACGE36_002866"
     CDS             complement(694..>1351)
                     /locus_tag="ACGE36_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385181.1"
                     /translation="QFLEELSGEEYRLFLNNNILKLISYAKKANSLSLVDNLDNLLPD
                     KLIYDENEDNSQKQIIIFELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPF
                     FTLINFMLYVKEHLGYDDLVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYK
                     EQNIKTLELIKKNIKYYDPLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF
                     "
BASE COUNT          483 a          205 c          202 g          461 t
ORIGIN      
        1 ctttatagtg tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa
       61 gtaggtaaaa attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca
      121 acgccaaatt gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc
      181 tggagcaacg attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca
      241 agatattttt attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc
      301 ttcagtcaat tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat
      361 ggctgaagca attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat
      421 tttggccatg attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat
      481 taaaattgca aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac
      541 aaaaaatcct caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat
      601 agctatttca aaaattgcta aaaactacaa tattactaga caaactattt acagaattaa
      661 gaatgatatt gagtataaca gtaatcaaat gaattaaaaa agttctttat acattaaaat
      721 ttcttctttt aactcatcaa ttctaggaga tctaacatca gaatcagcaa ttgcatttaa
      781 taacttatca atattttctt tattcgctag taaaggatca tagtatttta tattcttttt
      841 aattaattcc agagttttta tattttgttc tttgtaatta gctagaattt cttctacagt
      901 ttcgcctgaa atcatagcca tatctgaacc agtattgatt cctttgaatg gataaggttc
      961 tttatcatat gcctttatca tttctactaa atcatcatat cctaagtgtt ctttaacata
     1021 cagcataaaa tttattaatg taaaaaatgg cattgaagaa tagtctggaa atataaattg
     1081 aaacagtttg ttcgcaaaat atttatcatc atcattgctt tctggtgaca ctgacaggct
     1141 gctaagccac tttaaataca attcaaatat aattatttgt ttttgtgaat tatcttcatt
     1201 ctcatcataa ataagtttat cgggtaaaag attatctaag ttatctacta aagataggct
     1261 attagctttc ttggcataag atattaattt taaaatatta ttatttagga ataatctata
     1321 ttcttctcca gataattctt ctaaaaattg t
//
LOCUS       DAXWLH010000317         1343 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXWLH010000317 DAXWLH010000000
VERSION     DAXWLH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.325"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..141)
                     /locus_tag="ACGE36_002867"
     CDS             complement(<1..141)
                     /locus_tag="ACGE36_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFF8385182.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRH"
     gene            288..911
                     /gene="lexA"
                     /locus_tag="ACGE36_002868"
     CDS             288..911
                     /gene="lexA"
                     /locus_tag="ACGE36_002868"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFF8385183.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            1091..1309
                     /locus_tag="ACGE36_002869"
     CDS             1091..1309
                     /locus_tag="ACGE36_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385184.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          411 a          262 c          250 g          420 t
ORIGIN      
        1 atgacgcata ccggaacgaa aagcttccaa atactctttg cgcaagttga tttgttcttg
       61 ttcttcggct tttgagagac cattttcctt ctttttacgt gcaagttcat tgatccgctc
      121 tattttttca ggtgacagca tgctcagccc tccttttact ctccttcata ttatataaaa
      181 actgaaaaaa aaacaactag ataaactaaa taaaatacga acattcgttt gatttagcgc
      241 tttttatatg gtactattta tttatcaagt gaaagaaggt gtaccttgtg gcccgacaaa
      301 ctgaatcaag acaaatcgaa gtcttacgat atatccatga acaagtaacc caaaaaggat
      361 acccgccgac tgtccgtgaa atcggggaag ctgttcagct ttcttctact tctactgttc
      421 acggtcatct tgcacggctt gaaaaaaaag ggtttatcca aagagatccg acgaaacctc
      481 gtgcaatcga gttgacaaaa caaggtttag aaaagattgg gattcgtcca acaactattc
      541 cagtacttgg cgtcgtaaca gctggagaac ccattttagc agtagaggat gcttcagact
      601 tctttccaat ccctcctaat ctttcttccg aagaaggcag tctctttatg ctgacaattc
      661 gtggagatag tatgatcaat gctgggatat tggatggtga caacgtgatc gttcggaagc
      721 aagaaactgc tcaaaatggc gatattgtca ttgcaatgac tgccgaggac gaagcgactt
      781 gcaaacgatt ctataaagaa aaaaactttt tccgtctgca gcctgaaaac gacttgctcg
      841 aaccaatcat cttagatcaa gtatctatat taggacgagt agtaggactt tatcgtagtc
      901 atatctatta attgctacta attaaaagaa aactttttaa aaagagtttg agacgttggt
      961 ctcaaactct tttttcgccc gggttcttct tctttcaaat ttttatcact tccctcttta
     1021 caaaccgaac aaatattcgt atactaaata tacgaacaat tgttcgcata aattaaagag
     1081 gtgagaattc atgaaatcaa tgagacgtct tggcttagta ttaactgttt tgtttttagg
     1141 tattattatt ggtaatttcg gcatacgtcc agctttactt gtgtttgttc cgttatttac
     1201 tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa tctgagcggc gcagaattca
     1261 tgaagatcat ttctatcatg agccctacta cagatccagg caatcataat aaggattgac
     1321 tgctaattat tcaaaagaca aga
//
LOCUS       DAXWLH010000318         1341 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXWLH010000318 DAXWLH010000000
VERSION     DAXWLH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.326"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(313..927)
                     /locus_tag="ACGE36_002870"
     CDS             complement(313..927)
                     /locus_tag="ACGE36_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8385185.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          433 a          266 c          222 g          420 t
ORIGIN      
        1 tctttcatca acgtgtatta agagacaaga aagaatccat atggattctt tcttgtctct
       61 ttttccacta ctttttattt ttctgacact acatattctt ataactcatt tctcataact
      121 gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca agcttaaaat caaagatgat
      181 ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt aatcctcatt aataatcgtt
      241 ttcttttttt caaacgctcc atttttatgt ggaacattat ttttttaagg aactttgtca
      301 acatgtggat aactattttc cacttgtgtc ctggactagc tgttttgctg taaaatacaa
      361 aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt ccgcagatcg ctggatctag
      421 atttggcaaa gtcaagactg catcatgtaa attggcgtgg ttgtccaaaa ccaaatcagc
      481 aaattcaaat aatcgcaaac cactgctatg ttgcgatttg atacttcgtg acaaatcaaa
      541 tccagtaaca atgattaatg gatgaccatt ttctttgatc cactgagcta gttcgatgat
      601 tgccgggttt cttccttcat tcgaaagaag aagaaaaatg tcttctgatt tgacagaaag
      661 ctgatcaata attgcctgtc ctgcaccttc tagcgtttca tagacacctc cggctggatt
      721 ttcaatcaat atacttggta caagatccgg ggtttgatcc caaaattcaa atgctacacc
      781 acttagtgtg cggctagcat atatctgcaa tctaccacct tgattaattt gtttcgcgat
      841 ccaagtgcca gcttgaagca tcagcggctc ttctatcctt aatttctctt ccaattggtg
      901 ataatagtca aaataacata aactcattta tctctcccgc ctagctatac ttgacatgaa
      961 tagaaacagc tgcctaatct aaggaagtac ataaaaaaac agtagttaga cagtaacaca
     1021 tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac gaatacgccc aataccttga
     1081 catagcaaat aaaacaacgt gcagaccatg gaaaatatct acataggggt agaattttca
     1141 ccctagatta ggtacaataa attaggtcga tgcactgcat tgactatggc aaaatgatcg
     1201 gtcagggtgt atgtgtgtga ctggtcattc cctgattctt agcgccaact aagggtcagg
     1261 gagccttttt ttattcagct gtttctatct gaatttattg taagtaatct cttatccaag
     1321 tgcaagcaag aaaatacaaa a
//
LOCUS       DAXWLH010000319         1334 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXWLH010000319 DAXWLH010000000
VERSION     DAXWLH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.327"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            86..1165
                     /locus_tag="ACGE36_002871"
     CDS             86..1165
                     /locus_tag="ACGE36_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8385186.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DAXWLH010000320         1309 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.328, whole genome shotgun sequence.
ACCESSION   DAXWLH010000320 DAXWLH010000000
VERSION     DAXWLH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.328"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..1283
                     /locus_tag="ACGE36_002872"
     CDS             <1..1283
                     /locus_tag="ACGE36_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8385187.1"
                     /translation="LKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQ
                     DPRGQFFTTNTTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGER
                     QKISIGCSLSLNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLK
                     ELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEI
                     TYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPF
                     LVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQR
                     LLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTC
                     NRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT          523 a          149 c          228 g          409 t
ORIGIN      
        1 tattaaagtt aatgaatgga cttattccaa gactttatga cggaaaattg gttggggaaa
       61 ttttatttga aaataactcg ctacaatcct taacacaaga aagtatatca aaaaatattg
      121 gctacgtaag tcaagatcct agaggacaat tctttacaac aaatacaacg agtgagttag
      181 tattttcgat ggaaaacttt ggatttacaa aagagcatat ggataaaaga atcaatgaat
      241 tagctgaatt attagatctt tcaaatatat tagataaaaa cattttttcg ctatcaagtg
      301 gtgaacggca gaaaatttct attggatgta gtttatcgct aaactctaaa ttaattatat
      361 tggatgaacc gtcttcaaat ttggacttca aagcgactaa gaaacttgct gacttacttt
      421 gtgatttaaa aaataaagga tttactattg ttatagctga acatcgtttt tactatttga
      481 aagaattaat tgatcaagtt ctatttgtta aaaatagaac tgtaaaaact acaactattc
      541 agttgttaaa aactgaaaac aataacgcat taaggaaatt tgacattttt aatagttcga
      601 ttgaagttcc ctatatcgaa aaggtaagtg cagatgtatt aaaagttgaa gaaattactt
      661 atgaaaatat tctaaaagac ataaattttg atgtcaaagt tggagatgtt attggtttgg
      721 tagggaaaaa cggtgtgggg aaaacaacct tactaagaat actcatgaag attattcgcc
      781 ctaccaaagg aaagataatc gaaaataaaa aaaatttgtc atcgccgttt ttagtaatgc
      841 aagaaatgga ttaccaattt ttcacagaat cagtgaggag cgagttagcc ctggggaatg
      901 aaatagttag taaagatcag caagaaaaaa ttttaaaaaa aatggaactt tataaaatga
      961 aagatcaatt accgtttgat ttatccggtg gagaaaaaca aagattattg gtttctatag
     1021 cttcattgtc aaaaactaat ttatttctat ttgatgaacc tacaagtggg ttggattata
     1081 ttaatatgga aaggattgct gaccttatta aagacttaaa agaaaagggt gcagtagtta
     1141 tagctactca tgatccggaa tttctttata aaacatgcaa tagaattatt tatttaaaaa
     1201 atggtggaat aaaaaaagat ataaaactta gagtagaaga ttcaactttg atagaacaga
     1261 tttttaatga cataacgaaa tagaattgag gagaatatat atgaaaaca
//
LOCUS       DAXWLH010000321         1306 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXWLH010000321 DAXWLH010000000
VERSION     DAXWLH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.329"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            74..>1306
                     /locus_tag="ACGE36_002873"
     CDS             74..>1306
                     /locus_tag="ACGE36_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8385188.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFR"
BASE COUNT          410 a          259 c          254 g          383 t
ORIGIN      
        1 taaaaaaata tagaaaagac accctgaaat cctttagaat taatttggct accaattcat
       61 aggaggattc aagatgtctc atactcagct tatcaaagat accctaaata ttctagacct
      121 aaatatctat ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc aaatttgctt
      181 gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt
      241 cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgtgtc
      301 tgagtatcgc acttatctgg agctgaagaa acaacgattt aaatgtaagt cttgccaaag
      361 aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc aaaaagttcg
      421 ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg ctcgtcagaa
      481 aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc cattgaatcc
      541 tttcaaacag actttgccaa aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt
      601 tgccagtgca atgagtttta tcatgatgga cggacaaagc catgcattat tggatattgt
      661 cgaaaatcga cgcttgcctt atttagaacg ctatttttct cggttttcat tagcgactcg
      721 tgaagccgtt caattgattg ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa
      781 gcttttcccg aatgctcagt tgatcatcga tcgtttccac atcgtccaac atattggcag
      841 aactttcttg aatcatcgag tgaaagaaac cacggtacga cttaaaggaa attcccactc
      901 tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa ctcctacaaa aagaagaagc
      961 aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt tgactgaaag
     1021 tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct atcagttata
     1081 ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg attgtttaac
     1141 tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt
     1201 tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat tggaatgctt
     1261 aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgc
//
LOCUS       DAXWLH010000322         1271 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXWLH010000322 DAXWLH010000000
VERSION     DAXWLH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.330"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..612
                     /locus_tag="ACGE36_002874"
     CDS             <1..612
                     /locus_tag="ACGE36_002874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385189.1"
                     /translation="EQMIQLKNERSLLNRLVRVSYDNQTRKLSFHDHEEIKGKYRVAK
                     YYKLVTDEEYSGWCEEEKISKEEFYFLIQHYGQHFHNDFSLQSCSYGWHEIESLPEGT
                     IVQNSYPKHLNETTKRLEEIVFEEKKRHEQLLSVKKGIMKFEYRSECLEFIKRIELLE
                     NHKDFLLNKEQFDQEEVMESYVQLYTEWIRFNRFMSQENLYTA"
     gene            915..>1271
                     /locus_tag="ACGE36_002875"
     CDS             915..>1271
                     /locus_tag="ACGE36_002875"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFF8385190.1"
                     /translation="MEDKKYSKIIKKIKGLLAIAKDENQDEESQSAFVLAQRLMLQYK
                     IDKNDVFDTEDEIEPIGEESVTIYKKLYWWERSLGSIIAENFRVKMFYNSKKEKGERK
                     SKSKIVFYGFGSDLELA"
BASE COUNT          497 a          142 c          256 g          376 t
ORIGIN      
        1 gaacaaatga ttcagctaaa aaacgagcga agtttattga atcgcttagt tcgtgtctct
       61 tatgacaatc aaacaagaaa attatcgttt catgaccacg aggagataaa aggaaaatac
      121 cgagtggcaa aatattataa actagtcacc gatgaagagt attcaggttg gtgtgaagaa
      181 gaaaaaatta gtaaggaaga attttatttt cttatccaac attacggaca acattttcac
      241 aatgactttt ctctgcaatc ctgttcatat ggttggcatg aaatagaaag tcttcctgaa
      301 ggaacaattg ttcaaaatag ttatccaaaa catttaaatg aaacgacaaa gcgtttggaa
      361 gaaatcgtat ttgaagagaa aaaacggcat gaacagttgc taagtgttaa aaaagggata
      421 atgaaatttg aataccgatc agaatgttta gaatttatta aacgaatcga actcttggaa
      481 aatcataaag actttctatt gaataaggaa caattcgacc aagaagaagt gatggaatct
      541 tatgttcaat tgtatacgga atggattcgt tttaatcgat ttatgtcaca agaaaatctc
      601 tacacagctt aattttagag ttttaaagct tccggtagaa aattttgggc tttgatattt
      661 gttttaagcg gtttgaaata gaaatttgat aaactacatg aaaagacatt atatagtcgt
      721 gtagagcgaa ttagtgggtt taaatatcgt ttgactactt gtagtcaagt gtgttaagct
      781 atttttgtag taataagaca taagaagtta aaggaagtta ttgtacttcc tttaatttaa
      841 gctaaaaaga ctacaagtag tcttttcggt tttgagaggt gaaaatatat aattaaactt
      901 ggtaggtgaa gaaaatggaa gacaagaagt attcaaaaat tataaagaaa ataaaagggc
      961 ttttagcaat agcgaaagat gaaaatcagg atgaagagag tcaatctgct ttcgttttag
     1021 cacaaaggct tatgttgcaa tataaaatag ataaaaatga tgtattcgat actgaagatg
     1081 aaatagaacc aatcggtgaa gagtcagtaa cgatctataa gaaactatat tggtgggaac
     1141 gatcgctagg atcaataata gccgagaatt ttagggttaa aatgttttac aatagtaaaa
     1201 aggaaaaagg agagaggaaa agtaaaagta aaatagtatt ttatggattc ggaagtgatt
     1261 tagagttagc t
//
LOCUS       DAXWLH010000323         1262 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXWLH010000323 DAXWLH010000000
VERSION     DAXWLH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.331"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(111..1205)
                     /locus_tag="ACGE36_002876"
     CDS             complement(111..1205)
                     /locus_tag="ACGE36_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFF8385191.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          373 a          268 c          196 g          425 t
ORIGIN      
        1 agcattttat tctagcttaa tttgacaaat cataacctat aaaaaaacaa aataaaaaag
       61 cagaaataac tggctatttc tagcggatta tttctgcaat aactaattat ctatttttta
      121 ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa taattcgtca
      181 tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt caaggcgatt
      241 tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc cccatgatgc
      301 atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg agtcatcgtt
      361 gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc aaacaagcag
      421 attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg ataatccgcg
      481 ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt tccaatagaa
      541 atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat ttcaagatct
      601 cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa ctcagcagat
      661 tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc tgaacaaaaa
      721 acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg agaacctaat
      781 aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt gcccgaactc
      841 atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc ttctgctcct
      901 gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa agataagtca
      961 tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat tacttccatg
     1021 cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt ttctttatca
     1081 tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg agctaaatcc
     1141 aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat tcctaatttt
     1201 cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca ttatagcaaa
     1261 ca
//
LOCUS       DAXWLH010000324         1262 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXWLH010000324 DAXWLH010000000
VERSION     DAXWLH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.332"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            163..438
                     /locus_tag="ACGE36_002877"
     CDS             163..438
                     /locus_tag="ACGE36_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385192.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            865..>1262
                     /locus_tag="ACGE36_002878"
     CDS             865..>1262
                     /locus_tag="ACGE36_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8385193.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWV"
BASE COUNT          469 a          215 c          185 g          393 t
ORIGIN      
        1 catttgcaat aaatcagaaa atagtggtcg tttatttgta ttcgctattc taataataaa
       61 taaccactat ttgatttata tatatttcag tatccttctt gttttcatat aaccaaaggc
      121 gctatgattt ccatatgaaa aaaataaagt caggtgaaaa aaatgaaact tgaggataaa
      181 aaagtcattt tgaagaaaat caaacgacta ccagaccaaa tagatggcta tacgatctgt
      241 tctttcaaaa aagacaggag tataaaagct tatacgcaaa acacagaaaa aggaataatc
      301 tatttcctac atgaagaagg ttacgatacc caaaccttta tgtttactga aaaaaaagaa
      361 tttcataaac agatgaaaaa actgattgaa aaagaatttc caagaagtca taaactctat
      421 gtgaatcagc aattttaact tctaactgtt ctttattaat taaaaatgtt ctgtaataaa
      481 ataaaacttt ataacaacta taacaaaacc gaatgcaaaa cagaagttct ataagttata
      541 atcatagttg ttaggtgatt catctagtaa aaacaccaga tttcaaattt cacacaattt
      601 aaactagatc aatcatcaac cttcttcaaa ttgttagaac cctttcccga ggagctgcag
      661 cataagcaaa aaaacaaaca ggaagccaaa tggctaagac attttttcta ttagtctagt
      721 tgtttctcgt cgcaagttta agactttaga aagttatttc taatgatgtt tgggttgttt
      781 tgatcaatgc ttatgcacag ctccttacct attatttatc tcaacagatc taacaaatta
      841 atggactatt taaggagttt ttttatgctt acgatctatt cttctcgcac acatagttac
      901 catctgatgg caaactggct gaaagaccat caaatgcctt acagagaacg aattttcaca
      961 cctaaacagc ctttaacaag gaaagagata ttttatttat tgtcattaac agaaaacgga
     1021 tttgacgatc tattagctaa acgctcgatt gattataaaa atttgaaaga ccaaatcgac
     1081 caatgtacga cctcagagct tgtcgatctg atcgtctcgc attttacttt gttacaacgt
     1141 cccatcatta ctgacggaaa gaaattatta gtcggattca atgaagatag catgcgtctt
     1201 tttatccctg tacaatggcg cagaagtcaa ctgaaatctg tctataacga ttttacatgg
     1261 gt
//
LOCUS       DAXWLH010000325         1253 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXWLH010000325 DAXWLH010000000
VERSION     DAXWLH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.333"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..>1253)
                     /locus_tag="ACGE36_002879"
     CDS             complement(<1..>1253)
                     /locus_tag="ACGE36_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFF8385194.1"
                     /translation="LLFWPAGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVS
                     SLMNLKYGYSENIKLIMWQLIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSL
                     YWFVIQYGIKIPIKYKYYPVRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYK
                     HLNLLFKIFFVINIFIQFSFIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKR
                     WLTALVVGLLSIIAMYYVTDITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDT
                     DSGDVSNLRFELWKSAIEIFKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIF
                     LVMAATGGLGLLTLLTFLFIQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFIT
                     ELILVSTIGSFIFWGYLGSIQKTIS"
BASE COUNT          478 a          207 c          174 g          394 t
ORIGIN      
        1 ttagatatag ttttttggat agaacccaga tatccccaaa aaataaatga acctatcgta
       61 ctaaccaaaa tgagttctgt gataaataat gccgaaactg ctattgtaag aatcattaaa
      121 atagcaaaaa agtgcttatc ttttactaga tttggttgtg tgaaacaata acgcaaggct
      181 ttaaagcatt ggataaataa gaaggttaac aatgtcaata gacctaatcc tcctgtagca
      241 gccatcacta agaaaataaa attatgagca gttaaagtat cttttcccat taatgtatat
      301 ggtagtttat catgtgcata ttcaatatag tagcctggag aacctccgga tatcggctta
      361 ctcttgaata tttcaattgc acttttccaa agttcaaacc ttaaattaga aacatccccg
      421 ctatcagtgt cagggcgttt taaatcaact ttattgctgc tagttgttcc tgatgaatca
      481 gatgtatctt tttttacttc tgtaatatgc ggaacatgtg cccatgcatg agcagttata
      541 tctgttacat aatacatcgc aattatactt agcaatccta ctactaatgc agttagccat
      601 ctttttaaag tattagtttt aagaaaacga ctactataac ttgtaaagaa actaaaaaca
      661 aaagttgtta ttaataaaca aactaatgtg gtgcgtgaac ccgataaagc aataaacgag
      721 aattgaataa aaatattaat tacaaaaaat attttaaata gaagatttaa atgcttgtaa
      781 gtgaccaaat aatatatagc aataatgatg acgattaatg atataaccgc accaaaattt
      841 ggatctgtat atacaccaaa taaacggttg cctaaccacc ctaaacgaac tgggtaatac
      901 ttatatttta ttggaatttt tatgccatat tgaatgacaa accagtataa tgatactaaa
      961 ttagctaaaa accataccaa tgaaagtatc caggtaaata ctttgaaaaa aaagttacta
     1021 ctatagtctt tgccaaactg ataaatcata aagaaaaaga ttaattgcca catgattaac
     1081 ttaatatttt cgctatatcc atattttaaa ttcattaaac tgcttactag catcgcgcca
     1141 ataaaagcaa ttaatagcca atcatatttt tttagatttt tttctctaaa tgcaataatt
     1201 gcatctataa tgatcaagac aactcccgct attccagcag gccaaaaaag taa
//
LOCUS       DAXWLH010000326         1246 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXWLH010000326 DAXWLH010000000
VERSION     DAXWLH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.334"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..98)
                     /locus_tag="ACGE36_002880"
     CDS             complement(<1..98)
                     /locus_tag="ACGE36_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFF8385195.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGL"
     gene            223..1008
                     /locus_tag="ACGE36_002881"
     CDS             223..1008
                     /locus_tag="ACGE36_002881"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFF8385196.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1001..1243
                     /locus_tag="ACGE36_002882"
     CDS             1001..1243
                     /locus_tag="ACGE36_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385197.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          476 a          197 c          191 g          382 t
ORIGIN      
        1 atcagtccca ataaataatt tttatattct gacgcatcca ttttactacg gagattatct
       61 gccgcactaa ataatttttg atttaattct gcacccaaaa agatacactc ctatctctta
      121 tttaaagttg ctatataccc tcttatttta gcatactccc ttttagaaaa agcaaaaaaa
      181 aaagaaaaaa tcttattctt gataacaagc tacgtgacgt atatgcctaa caaaaataga
      241 acttttgaat caatcttaaa tgaaggttta accacagaac cggcatccat tatttttgat
      301 gaagttatcg atgacgttga agatagtaag tatcataaaa atttaaaaga acttttttat
      361 tttgcactca acaatggacc aagtttgttc actggtatat cagtacctga tagtgataat
      421 ccattaatta tggcaaaaat ttacttaaca aaatggtgta ataaatacat cagggatagg
      481 aataacccag cattaaaaaa acctcttaaa acatttggcg aaaaagatgc tgctttaact
      541 actagagtag cagcaaatgc taatattgat gatcaaaaag tattgaatga ctacttgcgt
      601 ggacattttt tatatatgag cgctgagaac atgaacggaa gtattttaga ggaatattta
      661 gccgaagtgt tagaacctga aggttggatt tggtgcgccg gctctgtata tcgagctgtt
      721 gatttctgtt atttaggaac ctcaccaatt cttttacaag tgaaaaataa atacaataca
      781 gaaagcagtt cgagttcagc tataagagta ggaacaacaa ttagaaaatg gaatcgttta
      841 aataagtcga caaaaatttc tggtttggat agccctatcc ccaattggaa agcattaata
      901 gaaatgactg aagctagtaa ggaactggca gctaagttaa ctgaaaatag ttatttagct
      961 tatattaatg aaaaatccac acgagaatta tggacactcg atgactaaca atatccttat
     1021 tagtatctat ccacgataca tggaaaaaat tataaacaaa caaaaaaact ttgaatttag
     1081 aaatttctat cttacctcag atccttataa tatcttttgg atttacgaaa caatccccac
     1141 caaagcttta aagtataaat tgattgtaaa aaatccaatt actaaactta agaaaaatca
     1201 acactacttt ttaggagacg atgagtttga acaattagtt taaaag
//
LOCUS       DAXWLH010000327         1241 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXWLH010000327 DAXWLH010000000
VERSION     DAXWLH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.335"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(19..210)
                     /locus_tag="ACGE36_002883"
     CDS             complement(19..210)
                     /locus_tag="ACGE36_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385198.1"
                     /translation="MNTLKDDFILAKAGSEEAIESILKRFSSLMHQQSWRNGRYDQDC
                     YQECMIAVYLAISKFEIKE"
     gene            complement(284..670)
                     /locus_tag="ACGE36_002884"
     CDS             complement(284..670)
                     /locus_tag="ACGE36_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8385199.1"
                     /translation="MSENDERVNAQLICFFEKVIKNTAYSYFERQKKIRELEQFDQFS
                     QHFFTENNIKIKQPVTILDVTFLIEDSRLAEIIPLLKEREQLFLIEKFVFDKTDKEIG
                     ESFGITRQGITNLKHRLYRKIQKLLI"
     gene            complement(731..1192)
                     /locus_tag="ACGE36_002885"
     CDS             complement(731..1192)
                     /locus_tag="ACGE36_002885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385200.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRRLSKDYYELKARLFALDNTNQP
                     NSLIRNEKLAKKSSTNISEEALYQQYICERGNKYRQLIPYSTISLTISSILKDAGRPL
                     SNKEIYKLLISDHKLNISYKNLTHNILPKMNVDNSVNVERAYRGYWQYRLH"
BASE COUNT          386 a          203 c          173 g          479 t
ORIGIN      
        1 aaggcctctc ccttgatttt attctttgat ttcaaatttt gatattgcaa gatatactgc
       61 aatcatacat tcttgatagc aatcttgatc atatcttcca tttcgccatg attgttgatg
      121 catcaaagaa ctaaatcttt taagaataga ctcaatagct tcttcacttc cagcttttgc
      181 caaaatgaag tcatctttca aagtattcat agaccgtcct cctttcctaa atttaaagtt
      241 atttagagga ggtggtcgta tacaaataat cgttaagtgg cttttaaata agtaactttt
      301 gaatttttct gtaaagacga tgttttaagt ttgtgatgcc ttgtctagtg atcccaaaag
      361 attctccgat ttccttatct gttttatcga aaacaaattt ttcaataaga aatagctgtt
      421 ctctttcttt taataaagga ataatctctg ccaatcttga atcttcaatt agaaaagtaa
      481 cgtctaaaat agtaactggt tgtttgattt tgatattgtt ttcagtaaaa aaatgttgag
      541 aaaattgatc gaattgttcc agttcacgaa tctttttttg cctttcaaaa taagagtacg
      601 cagtattttt aataaccttt tcaaaaaaac aaataagctg tgcattcacg cgttcatcat
      661 tttctgacat tctgctcact tcttccaata aatatttaaa aggaaaaggg acagaaagcg
      721 aaaaaagaag ctaatgcaat ctatactgcc aataacctct ataagctctt tctacattga
      781 cactattgtc aacgttcatt ttgggtagaa tattatgagt taagtttttg taagaaatat
      841 taagtttgtg atcgcttatt aatagtttat atatttcttt atttgataat ggtcgaccag
      901 catcttttag aattgaggaa attgtcaaac tgatagttga gtatggaatc aattgtctat
      961 atttatttcc tcgctcgcaa atatattgct gatacagcgc ttcttcagaa atatttgttg
     1021 acgatttttt ggccaatttt tcatttctaa ttaaactatt aggttgatta gtattatcta
     1081 atgcaaaaag tctggccttt agctcataat aatctttact aagtctcctc ctttcttcga
     1141 gtatcattct catttgtgta taaattgcat ctttttcttc ttctagtttc atctacttct
     1201 cctttcatga ttaacaacta aaaatatttt taaaaggaca c
//
LOCUS       DAXWLH010000328         1241 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXWLH010000328 DAXWLH010000000
VERSION     DAXWLH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.336"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            150..1190
                     /locus_tag="ACGE36_002886"
     CDS             150..1190
                     /locus_tag="ACGE36_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8385201.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          501 a          178 c          205 g          357 t
ORIGIN      
        1 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
       61 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      121 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata
      181 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      241 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
      301 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
      361 aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
      421 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
      481 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
      541 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
      601 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
      661 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
      721 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
      781 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttgtccctg
      841 aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg
      901 tccagactat tcataatgca aagaaaaaag ctgaaataga aagtgatatt tcgatagttt
      961 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1021 aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct
     1081 atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt
     1141 tgaattatgc aagctcccca agcactatta cgaaagactg gttagagtga aactttactg
     1201 acaaagtgat tgttgcttca tggagtaaca ttaaaattaa a
//
LOCUS       DAXWLH010000329         1235 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXWLH010000329 DAXWLH010000000
VERSION     DAXWLH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.337"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            158..742
                     /locus_tag="ACGE36_002887"
     CDS             158..742
                     /locus_tag="ACGE36_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFF8385202.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            856..>1235
                     /locus_tag="ACGE36_002888"
     CDS             856..>1235
                     /locus_tag="ACGE36_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFF8385203.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTV"
BASE COUNT          503 a          183 c          224 g          325 t
ORIGIN      
        1 aaaatattct tacgcaaggc aatatgtatg aatacatcaa gaattattaa aaaaaccgat
       61 ttcagcaaaa cattaaaaaa gcccgtgctg gcacacggac tcatacctca tttctgagat
      121 cgcaaatata ttataacaag aagtgaggaa tatttaaatg gcaaaaaaag ttatgggtca
      181 agacggaaaa atgtataaag ttagtaaacc tttttacaaa aaagtatggt tttgggtatt
      241 agcagtaatt ttaattataa ttattggttc tgctttaaat ggtggatcag atagtaataa
      301 agcaagtgat aatggtggcg aaaaagtaac taaatcttca acctctgctt catcttctaa
      361 agaagaaaaa agcgatacct tctataagat tggtgacact gtaaaagttg gtgatgctga
      421 atatacacta aatagtgtag aactaactga tgaaagaaat caatttgaag aaaaccaacc
      481 tgcacaagta gtaaaaatta cttataccgt aaaaaatgat ggcgattcag atatccctgt
      541 aggtacagat gtggaagtat atggaccaga tgacaaaaaa tcagagacat atgccaacga
      601 aaatacaatg ggatctgttg ctccaggaaa acaaatggat gtaactgctc atttcacgtt
      661 aaaccaaaag ggagaaatag aaatccactt ctctccttta gtatcatttg aaaaagcagc
      721 tatttttaaa gcaactgtat aataaataaa aaacacgccc accgtccaaa gcaagcgtgt
      781 tctaagaaaa acaaacctat acagtaggct tctttatagt tcctattgta tcagagaaag
      841 agagctgatt caattatggc aagattagtc aaacgtggaa atagttggca atacgaaatt
      901 tcatacaaaa aagatgacgg aaaatacacg aagataagaa aatcaggatt taagacaaaa
      961 ggagaagcaa aagatgccgc caacgaatta gaatataact tgaacaaagg ccttaaaggg
     1021 gatcgcaaaa atctattatt atcagattac tttgaggatt ggatgcaact ttataaagaa
     1081 ggaacagtat ctcctatcac ttatagaaaa tacgaagata cgttaatgaa cataaagaaa
     1141 tatatgccag cggtattgat ttctgattta gatagagtgg gatatcaacg ctttttaaat
     1201 aagtatgcta aagaccatgt aaaatctacc gttat
//
LOCUS       DAXWLH010000330         1231 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXWLH010000330 DAXWLH010000000
VERSION     DAXWLH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.338"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..695)
                     /locus_tag="ACGE36_002889"
     CDS             complement(<1..695)
                     /locus_tag="ACGE36_002889"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFF8385204.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHV"
     gene            complement(667..>1231)
                     /locus_tag="ACGE36_002890"
     CDS             complement(667..>1231)
                     /locus_tag="ACGE36_002890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385205.1"
                     /translation="DKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMK
                     IAFANSSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHV
                     GDGENLKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAI
                     AFETDKLSRFRAAIRGVRDGYRGVIGD"
BASE COUNT          449 a          217 c          160 g          405 t
ORIGIN      
        1 acgtgagaaa tcgatgtact gtttctatca aaatatgcat catacttcgt atttataaat
       61 attgaactga tataactttg tccaatagag aataggagca agctaaggat tgtaacatat
      121 ccagtaatct ttttattaat ttttacaata aaaaaaggga gataaaaata tgcaagatta
      181 tggaacagtc cagccactaa gataaaaaaa atatacttaa atatagcttt gctgcctcgc
      241 aatttagtta gatgataaaa agctagtaat atgaatatca atgcgctaaa catacgtact
      301 tgtattgctt gtgaagtaaa accaaaaata aaaaacaaga acaaaactaa aaaaacatta
      361 gttgtgaata atctgatgac atacaataaa gctgagagta agacagtaga aataatcaat
      421 aaaaaaatct gatactgact ggaaataaag gtacatacgt acataacagc tctgaatccc
      481 ggttcaatac tatctgaact aaagtccata ccaaattgtg ctatcccatt atacaagaca
      541 gaatatgcat aataatcagc gttttgtgtg ttaaatgcca tgatgatcca cattatcaaa
      601 agtagcaccc cagacaaaat cttgttcgat gtactccatt ttttttgtga aacactcttt
      661 tcttgattaa tctccaatga cgcctctgta tccatctcga acccccctta tagccgctct
      721 aaatctactg agcttatcag tctcaaaagc tattgcaata aaaaatttca ataatctttt
      781 attcataagg cgggaaaatt ccttatcact tttatacatt cttttcacca atgttgaatt
      841 tcttgcaata aaatactttc gtgttgaatt atgattgtct attttaattt tttttgatcc
      901 tatttttaaa ttttctccat ctccaacgtg atgataaaaa tagacttgat ttgttacata
      961 aatgttatag ccattatttg caaccctttt acaaaattca acatcaatct gatcaataaa
     1021 aaattcatcc ttcatatttc caacatcgtc aattaattca cttttgaaaa acattccaga
     1081 ggaatttgca aatgctattt tcatggaacc actagttgaa gcaaagtgtt tttttacgta
     1141 tctccctctc tcatttttgc aggtaaaaga gacagattca tcattgctat tctcaaagta
     1201 attgggaaca ataatcccaa tgtccttatc t
//
LOCUS       DAXWLH010000331         1197 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXWLH010000331 DAXWLH010000000
VERSION     DAXWLH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.339"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            10..483
                     /locus_tag="ACGE36_002891"
     CDS             10..483
                     /locus_tag="ACGE36_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFF8385206.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            492..719
                     /locus_tag="ACGE36_002892"
     CDS             492..719
                     /locus_tag="ACGE36_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385207.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          376 a          193 c          203 g          425 t
ORIGIN      
        1 gggataaaaa tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc
       61 gtatttattt tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc
      121 aattttgatt ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg
      181 atcgttattg cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt
      241 tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc
      301 gccttttttg gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt
      361 ttgatgttgg taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc
      421 gtgctggaaa aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg
      481 tgaggtgcga catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga
      541 gtgtgactga attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa
      601 atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt
      661 gtcaaccagg tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa
      721 ataaagtaga acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta
      781 gaagttattt acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata
      841 aataagatat gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt
      901 tcctgataca actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt
      961 attcattcca ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg
     1021 ccattctgtt tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact
     1081 cttactccat tcttttttag taagctccca tatatactgt aaagccttct atatattatg
     1141 tcatgttgct ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagt
//
LOCUS       DAXWLH010000332         1181 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXWLH010000332 DAXWLH010000000
VERSION     DAXWLH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.340"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>1181
                     /locus_tag="ACGE36_002893"
     CDS             <1..>1181
                     /locus_tag="ACGE36_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385208.1"
                     /translation="FHMSESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGID
                     PAYCYYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFL
                     ALYGLSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTF
                     FKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILR
                     PNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTV
                     FDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQF
                     YMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLI
                     V"
BASE COUNT          403 a          190 c          194 g          394 t
ORIGIN      
        1 ttccatatgt ctgaatcaaa ttttttacta tacatcaaag aattagaaca agactttgag
       61 cgtctcaatc taacagaact gcatattgat aagcaaaaac cttttttaaa gttgaatttt
      121 gaaggaattg atccggctta ctgctattat cgtctatttg gaagatactg caacgaatca
      181 gtcagttatc agatcttgac ttctttgttc agctgtcaga caaattcgat tatcagttta
      241 agtcagcaaa caaactatag tgcctcttat ttgtatacga aaatgaaaaa gattaatgca
      301 tttttagcat tgtacggttt gtctatctcc ttttctaata atggcagaaa gtctttttct
      361 ggaaaagagg tacaaattca atatgcagca ttagatattt attggaacat cttttcaagt
      421 actgctacag aattttatga tgaagatccg caagtagtgg cttttatgat gaatactttt
      481 tttaaaaaag aagtacttga ccgactaaat agcggtatga tagataaatt atattttcta
      541 ttaaaaatat gtaaagagaa ttttcctgca acttctgtga aagacgtaca aaaagaattc
      601 acagagtatg aatatattga cttttttatt gatccctctg tagatatttt gcgacctaat
      661 ctgccaattt gtcgcgaaca acgcatctta gtgaatattt tagctcgact ttctatcacg
      721 aaaatcgaat cggaagaaac aagcttggag caatacaaac ttttaaaaaa agcaaacgtt
      781 cctcatgttc tttattcaag agaaatggtg gagtcctttt gtactgtatt tgatttgttt
      841 atacctgaaa aagaaaagat tctttactca ttggcattcg cacgaaataa actttataat
      901 ggatttctta acttcaatca accaaatacg ccccttccta ctttcatgct ttatcaagaa
      961 catagcgtgt tgaaagaagt ccaaaccgcc attgaacaat tttatatgga tttcagagaa
     1021 caaaatcaac acttgttacc agttatttta gaaaaggaaa atgtgcaatg gatggttgaa
     1081 gacctagtac atttgtatga tcgctacaaa aaacagccgc gaattgtgat tggtgtgaac
     1141 tatacgcgtg atttttatat cagtaaagat ctgatcgtta a
//
LOCUS       DAXWLH010000333         1136 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXWLH010000333 DAXWLH010000000
VERSION     DAXWLH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.341"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..355
                     /locus_tag="ACGE36_002894"
     CDS             <1..355
                     /locus_tag="ACGE36_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385209.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACGE36_002895"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACGE36_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1136
                     /locus_tag="ACGE36_002896"
     CDS             985..>1136
                     /locus_tag="ACGE36_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8385210.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          430 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggt
//
LOCUS       DAXWLH010000334         1128 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXWLH010000334 DAXWLH010000000
VERSION     DAXWLH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.342"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            154..417
                     /locus_tag="ACGE36_002897"
     CDS             154..417
                     /locus_tag="ACGE36_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8385211.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="ACGE36_002898"
     CDS             417..689
                     /locus_tag="ACGE36_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8385212.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="ACGE36_002899"
     CDS             766..1095
                     /locus_tag="ACGE36_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8385213.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          428 a          142 c          222 g          336 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa acatgtcatt aagtattt
//
LOCUS       DAXWLH010000335         1122 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXWLH010000335 DAXWLH010000000
VERSION     DAXWLH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.343"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            2..631
                     /locus_tag="ACGE36_002900"
     CDS             2..631
                     /locus_tag="ACGE36_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8385214.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(745..1119)
                     /locus_tag="ACGE36_002901"
     CDS             complement(745..1119)
                     /locus_tag="ACGE36_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8385215.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
BASE COUNT          377 a          203 c          161 g          381 t
ORIGIN      
        1 tatgaaaatt gtttttttgc atggtctagg ccaagacgaa caatcatgga aagatgtaat
       61 ctcttatcta cccaatagtt attcttgcaa aagtctttca ttgttcaatc atttaaacag
      121 cacagacact gctactttag acgacttaac caaatacatc gaagtacaat tgaatcagat
      181 aaaggaacct tttattttat gtggattatc tttaggcgct gttattgctt taaactatct
      241 tgcaactcct tctccatatt taaaaaaggt tgttttagtc gctccacaat ttgagtctcc
      301 taatcgctta ttgcttactt tccagaatat ttatttttac tgcgtacctt ctaagatatt
      361 tgaaaaatta ggtctatcta aaaaacaaac ccttacccta cttcgttcac ttgaaacatt
      421 agactgcaaa aaacagcttc gtacatcacg acttcctaaa ttgattttat gcggtaccaa
      481 agatcatgcg aataaaccag cagcaaaacg tttatataaa ctttcaacga atggccggct
      541 actctttatt aaaaacggaa aacatgaatt gaatagagaa aagccagaaa taatggcaca
      601 agctatgcga tcttttatcg aaaatagtta atttaaaaaa agacgacaaa atgtgtcgtc
      661 tttttattta ccatggtaag aatgttgtct tgttttttca atacagcttg attgagacta
      721 gttttatgga caatagagag aaagttattg ttttattcca gagaattttt ctggtcgtag
      781 atctactaat tgaacatgac tgtctaaaga accacttggg ctttcaagag taaatacacc
      841 tagacgtgga tctaaagaaa gggtcatatg ttcatccatg tacatttttg aataatcttt
      901 tatataaaca tccccaatat cactatctaa ttggagttta taatcatttt ttctctgttc
      961 tttatcaaga agaagtaaac gaaaactagt atctaaagag caaacaccca ttttagaata
     1021 ttccccaacg ccatcatcta aatccaatat aactgttgcg ccttcatcaa tatatttttg
     1081 taatctttct tgtgcttgtt ccgtaatttt aagatacata ac
//
LOCUS       DAXWLH010000336         1104 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXWLH010000336 DAXWLH010000000
VERSION     DAXWLH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.344"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            576..737
                     /locus_tag="ACGE36_002902"
                     /pseudo
     CDS             576..737
                     /locus_tag="ACGE36_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(785..904)
                     /locus_tag="ACGE36_002903"
                     /pseudo
     CDS             complement(785..904)
                     /locus_tag="ACGE36_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(925..>1104)
                     /locus_tag="ACGE36_002904"
     CDS             complement(925..>1104)
                     /locus_tag="ACGE36_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385216.1"
                     /translation="FFCCFCVGILWYIGGNEKKIHGVVTNISADEFVFEDEKQNEYSI
                     QKRSSLSNDVGKEKS"
BASE COUNT          396 a          209 c          144 g          355 t
ORIGIN      
        1 tagtcgaaat tttgttgtac atttttaaaa ataacaaaaa ttttttatga taattctgtc
       61 tttttctagt ttccttatgg cgtaagttta acgaataaga atttagtatc tatactttta
      121 tattacaaaa aatattcgta aaattcgggc tctatacttt tcgatgtttg tgaactttat
      181 caacatcttt tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat
      241 ttgcccaaca aattcatagg aggattcaag atgtctcata ctctgcttat caaagatacc
      301 ctaaatattc tagacctaaa tatcaatatc catttcgagg aaaattgttt aactaaagaa
      361 aaatacaaag gacaaatttg cttgatttac aaagggactc ttcagtacac accagaagaa
      421 tatccacatc ttccaatata ttaaaagaac cttcttgaat cattgagtga aagaaaagag
      481 attcgtcact atttgaggac tgtttaacta gagacattag cgatctgcct gaaacctata
      541 caaccacttt acgaacgttt aaaaattttc tgccacagat tcaaaatgcg cttaactgca
      601 gctattcaaa cagaccattg gaatgtttaa acaaccacat caaagtatta aaacgcaata
      661 cgtatggctt tcgcagcttt tacaatttca aactacggat catgattcgt catggcaaaa
      721 catttttaac caaataaaca gaagaatgtg ggataaatct aaaaatcagt tttgctacag
      781 actctcgagt aaacggcggg agcagaagca actccttcgg aaataagccg aaattcacaa
      841 aaatttgaaa agcaattttc gtgaatttct tcttatttct cggagttaaa cacttctgtc
      901 ccagcctctt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      961 gcttcttttt tgaatactat attcgttctg tttttcatct tcaaatacga actcatccgc
     1021 agaaatattt gtcacaactc catgaatctt cttttcatta ccacctatat accacaggat
     1081 tcctacacag aaacaacaaa aaaa
//
LOCUS       DAXWLH010000337         1100 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXWLH010000337 DAXWLH010000000
VERSION     DAXWLH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.345"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            38..976
                     /locus_tag="ACGE36_002905"
     CDS             38..976
                     /locus_tag="ACGE36_002905"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFF8385217.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
BASE COUNT          424 a          133 c          203 g          340 t
ORIGIN      
        1 agaataaata gcacttaaaa atagagatga gggatttatg ggaagacaag aattatatag
       61 tttagctatc aatgaattgg aaaaaagaaa tcttgaaata catgaattaa atcaaaaaaa
      121 taattctatt ttaggcatga aaatacgaaa taaaattaat caacttaacc aaatgaaaaa
      181 acgagataat ctgttgccag caaaatatac ccttaaatcg atgaccggta aggggcctat
      241 taaagactat tcaaacagag agaatcgcag tgatgaacta agaaaaaaag agttaactaa
      301 tcaaggaaca cctaaaagga ttgctgtgta tagttgtatc actggtaatt atgattctat
      361 taaagaaccg gatttttctc atccattggt agattactat ttgtatacag ataatgtgat
      421 tacaagtgag cattggaata ttagagagat acctaaatat atatcggaat taaaaaataa
      481 tattttgatc aatagatata ttaaatttca tcctaatgag ctatttactg actacgattt
      541 tactttgtat gttgatggta atgtaacaat aacttctgat attaccggat tactgaacag
      601 ttcttccaaa cttgggttaa gtatgcataa acatagtgaa agaaaatctt tatattctga
      661 ggcagatgca ttaattgcag ttggcaaagg taataaggaa agtattatag agcaaattag
      721 taagtacaga gaagaaggat ttcctgataa ttttggattg gctgaagcca cggttatttt
      781 agttgattta aataattcaa tcgctgagaa tcttctagat ttgtggtgga gagagtattt
      841 gaactcagaa agtgggcgag atcagctcgc atttccttac gtgttgtgga agtccggaca
      901 tgcaatgaag gaagtgacta ccttaggcaa taatgtatat aaaagcgcta aattaagaat
      961 cataagtcat tattagccct aaatgatttc taaactattc tttcaaatcc tagtaaatat
     1021 ttggagatag aagatttcct ttttaaagtc ataaaaaatt aagttatatt ttttgagtga
     1081 atggagaaga cgcatagtat
//
LOCUS       DAXWLH010000338         1073 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXWLH010000338 DAXWLH010000000
VERSION     DAXWLH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.346"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            183..692
                     /locus_tag="ACGE36_002906"
     CDS             183..692
                     /locus_tag="ACGE36_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385218.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT          392 a          163 c          182 g          336 t
ORIGIN      
        1 ctaagtaaag attgacgatt tgttgcttga attcttgtga aaaagttctt cgtgttctct
       61 tagacataaa aattcctcct ggtatgtttt cttctagtct acacacctta atttttctgt
      121 ctagttaatt gtagcctatc caaaagtaag gaattacaaa aagagaaaga agagaaggaa
      181 gaatgaaaaa ggtattattt gcggttagtt tagttgttaa tatagcacta gcaggaggat
      241 tattttatac cattacagaa atgaataagc ttgatgacca acgggaaagc ttagcaatca
      301 gtaacgaaca atacaaaagg aagctagatg aagctagcag caccaacgtg gacactcaag
      361 aagccgtaga atcctctaca attgtttata gcaatgaatc cagtgaaacc ctaacaaatt
      421 cggaaagaag cacagctcca gctacagaaa ctgtttctca aaaagctact ataacaatag
      481 gaagtaaagt attcacgttt atagtaaatg gcttgacaat agaacacgat tctagcagaa
      541 caggaaaaat gaccgagctt tctcatgagt ttccagtgat tgttactact cctagtgcta
      601 cccttaacgg tatattggtg caagacaaca atgataataa ttgctatata atggtagacg
      661 gtcaaactct atacagagga acataccaat aaattttgag tcttaatatg actgatattt
      721 ataaaaaatt cctgatatta gaacaagaat acgctgtctt caaatgtata atattacaat
      781 agcacgatat agcaatattg ttatatcgtg tttcataata attaagagct ttaaaattag
      841 tatttattag atacttaaac gctattttag tattgataaa ttgttaccag caacaaaaaa
      901 gaagttactt agtgctaact tttgagcaac tgctagagga ggtaggatat ggctaattat
      961 cctgtaattt ttattgtatt ttctagaaat gtaattattc tcttaaacat tccagcatat
     1021 ccaagtatat agaaaattta cgttttctat atacttggat gtttacaagt tat
//
LOCUS       DAXWLH010000339         1045 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXWLH010000339 DAXWLH010000000
VERSION     DAXWLH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.347"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            85..451
                     /gene="ssrA"
                     /locus_tag="ACGE36_002907"
     tmRNA           85..451
                     /gene="ssrA"
                     /locus_tag="ACGE36_002907"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            694..996
                     /locus_tag="ACGE36_002908"
     CDS             694..996
                     /locus_tag="ACGE36_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8385219.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDVVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          329 a          183 c          197 g          336 t
ORIGIN      
        1 atgattcgct tttttattga atttatccca atatatgcta tactaatttt gtatgaaaaa
       61 tacacgtaac ccttgttagc taacggggac gttacggatt cgacaggcac agtcgagctt
      121 gaattgcgtt tcgtaggtta cgtctacgta aaaacgttac agttaaatat aactgctaaa
      181 aacgaaaaca actcttacgc tttagctgcc taaaaacagt tagcgtagat cctctcggca
      241 tcgcccatgt gctcgagtaa gggtcctaac tttagtggga tacgtttcaa ctttccgtct
      301 gtaagttgaa aaagagaaca tcagactagc gatacagaat gcctgtcact cggcaagctg
      361 taaagtgaat ccttaaatga gttgactatg aacgtagatt tttaagtggc gatgtgtttg
      421 gacgcgggtt cgactcccgc cgtctccatt gtgagatttc gccagatttc gctagatgct
      481 taaatcctta ttttataagg gtttgagcat tttttgtttt cgctgatttt cgttaaatga
      541 atgaaaatgg cattcaaaat ggcattcaaa atggcattca aaatggcatt caaaaaaaca
      601 tcttttaata tatatgttcc atccatattg ccaacgctat tcctgcttta caaaaagaac
      661 attagttcgt atactctttt tgaggtgaac attatgttga tggaaggaaa gacacaatta
      721 tggtttaaat ttgatccttc gaatagattt atcaaagatt tttataaagt atgggattca
      781 gaagttttct ttttagcaat cgaagatagc ttattaatca atctctacta ttctaataag
      841 aactatttca aaattcctgc tgcgaaaact agaatgaaga aagacgtata ttttttgttt
      901 gatgtcgtga ctgacgtgcc agacgcacga agcgatcatc ggcgttatga ctatataaag
      961 tatactttcg ttgatccaga aagatacaaa gattaaaata ggctacctaa aaaggtagcc
     1021 cggaacggat tttatcacca tactt
//
LOCUS       DAXWLH010000340         1016 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.348, whole genome shotgun sequence.
ACCESSION   DAXWLH010000340 DAXWLH010000000
VERSION     DAXWLH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.348"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(245..793)
                     /gene="aac(6')"
                     /locus_tag="ACGE36_002909"
     CDS             complement(245..793)
                     /gene="aac(6')"
                     /locus_tag="ACGE36_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8385220.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          298 a          213 c          153 g          352 t
ORIGIN      
        1 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag
       61 ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac aacctctggt
      121 acacggtgtt ccaaaagtag cttcttcgga aataagttgg aatttcacaa aaattcaaga
      181 agtaattttc gtaaattcct tcttatttct tgaagctgat tacttctgtc acaacctctt
      241 tttattattg agaatttggt cgaggaataa tcgtttttgc catccaaata tccggtttgt
      301 cccagccatt tgcatttggt aagacaccta cgattttata acctaatttt tcatagaatt
      361 catacggatg ttcacgaagg ttctggatgg aagccacttt atcaaatgta tgctcataca
      421 ggtccgtttg acttaacgtt gttccatggt ctaaatcatc cgtacctaaa taaatcgtga
      481 ttcctcctct ggaagctact tctttttcta agtaattgac taatcgagta cctatttggt
      541 tctttcgtcg ggagctttct acaactaatg gatgcaattc ccaacctgtg ataccgtatt
      601 gagggattgc accaataaat cctactaact catcttggtc taccgctgct accgcgattc
      661 gttctggatt catcatttct tctacttctt ctgccgagct gtctccatat tcttccggcc
      721 aagtcagtct cagtaaatca gaaagctgat ctttcaatac tggattatta cggtcaaatt
      781 cactgattat catacgtctt cccccttgtt cttgcttatt ctaacagtcc ttttgaaaaa
      841 acgaaatgtt ctcgctcaaa aatctttagc taccaatcag tatctctatt ctttcattat
      901 ttatcttact tataaaataa aaaaattata cagaaacact ttattcaaaa taggaaagat
      961 caggaaagcc atcaattgga ggaaaaaact ctgtttgttc atcatgatat agatta
//
LOCUS       DAXWLH010000341         1015 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXWLH010000341 DAXWLH010000000
VERSION     DAXWLH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.349"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ACGE36_002910"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ACGE36_002910"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="ACGE36_002911"
     tRNA            203..276
                     /locus_tag="ACGE36_002911"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="ACGE36_002912"
     tRNA            316..405
                     /locus_tag="ACGE36_002912"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="ACGE36_002913"
     tRNA            420..491
                     /locus_tag="ACGE36_002913"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="ACGE36_002914"
     tRNA            507..579
                     /locus_tag="ACGE36_002914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="ACGE36_002915"
     tRNA            589..661
                     /locus_tag="ACGE36_002915"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="ACGE36_002916"
     tRNA            697..772
                     /locus_tag="ACGE36_002916"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="ACGE36_002917"
     tRNA            774..854
                     /locus_tag="ACGE36_002917"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="ACGE36_002918"
     tRNA            861..934
                     /locus_tag="ACGE36_002918"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="ACGE36_002919"
     tRNA            937..1009
                     /locus_tag="ACGE36_002919"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          240 a          228 c          272 g          275 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc ttttt
//
LOCUS       DAXWLH010000342         1006 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXWLH010000342 DAXWLH010000000
VERSION     DAXWLH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.350"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>1006
                     /locus_tag="ACGE36_002920"
     rRNA            <1..>1006
                     /locus_tag="ACGE36_002920"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          313 a          199 c          289 g          205 t
ORIGIN      
        1 acgtaagcta tgatccagag atttccgaat gggggaaccc aacatcttta ataggatgtt
       61 acgattgtgt gaatacatag cacattcgag gtagacgcag agaactgaaa catctaagta
      121 cctgcaggaa gagaaagaaa attcgattcc ctgagtagcg gcgagcgaaa cgggaaaagc
      181 ccaaaccagc aagcttgctt gttggggttg taggactcca atatggtagt tctttcagat
      241 agtcgaatga cttggaaaag tcagtcaaag agggtaaaaa ccccgtagac gaaatgtgga
      301 agacacctag gaggatcctg agtacggcgg aacacgagaa attccgtcgg aatccgggag
      361 gaccatctcc caaggctaaa tactccctag tgaccgatag tgaaccagta ccgtgaggga
      421 aaggtgaaaa gcaccccgga aggggagtga aatagaacct gaaaccgtgt gcctacaaca
      481 agtcaaagcc cgttaatggg tgatggcgtg ccttttgtag aatgaaccgg cgagttacga
      541 ttgcatgcga ggttaagttg aagagacgga gccgcagcga aagcgagtct gaatagggcg
      601 tttgagtatg tagtcgtaga cccgaaacca tgtgatctac ccatgtccag gttgaaggtg
      661 cggtaaaacg cactggagga ccgaacccac gtacgttgaa aagtgcgggg atgaggtgtg
      721 ggtagcggag aaattccaaa cgaacttgga gatagctggt tctctccgaa atagctttag
      781 ggctagcctc ggaattgaga atgatggagg tagagcactg tttggactag gggcccatct
      841 cgggttaccg aattcagata aactccgaat gccattcatt catatccggg agtcagactg
      901 tgagtgataa gatccatagt cgaaagggaa acagcccaga ccaccagcta aggtcccaaa
      961 atatatgtta agtggaaaag gatgtggggt tgcacagaca actagg
//
LOCUS       DAXWLH010000343          995 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXWLH010000343 DAXWLH010000000
VERSION     DAXWLH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.351"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            289..606
                     /locus_tag="ACGE36_002921"
     CDS             289..606
                     /locus_tag="ACGE36_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFF8385221.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..>995)
                     /locus_tag="ACGE36_002922"
     CDS             complement(697..>995)
                     /locus_tag="ACGE36_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385222.1"
                     /translation="LLDFRYHSVSGFITLIFLSLFAGYYFQRNRRIISLFIANIISTV
                     TSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVPQILGSLWAVALSYKKARHP"
BASE COUNT          367 a          124 c          162 g          342 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagta
//
LOCUS       DAXWLH010000344          987 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXWLH010000344 DAXWLH010000000
VERSION     DAXWLH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.352"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            20..964
                     /locus_tag="ACGE36_002923"
     CDS             20..964
                     /locus_tag="ACGE36_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8385223.1"
                     /translation="MDTILITGGAGFTGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          325 a          150 c          210 g          302 t
ORIGIN      
        1 tgcaattgga gtgaatttga tggacactat attgattact ggcggagctg gttttaccgg
       61 ttcaacgcta gcgaattatt tgggaaaaga aaacaaaatc gtagtagttg acgatttatc
      121 aatgggaaaa aaagaaaact tggatatgga taaacaagtg actttcattg agggggatgt
      181 atcagattct caattgatgg aaagaatcat gaaagaatat cagtttgcct atatttttca
      241 tctagctgct gtagctagtg tggcagattc tgtagaacgg cctgtagaaa cacatcgtgt
      301 aaattttgac agtgcgcttt tattgcttga gttagcacgg aaatatcaat ctaatttaaa
      361 acgattagta tttagttcat cagctgcagt ttatggtgat gaaccaacac tgccaaaaaa
      421 agaagaatca gtcatccgtc cattaacacc atatgctatc gataaatttg cagcagaaca
      481 atatgttttg gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa
      541 cgtttatggt ccaaatcaaa atccaaactc tccatatagc ggagtcattt cgattctagt
      601 ggatcgttac aaaaaacaat tggcaggaga aaaagcggaa tttactttat ttggcgatgg
      661 gagtcagtct agagactttg tttatataga agatgttata caagcacttt tacttgttgc
      721 caaggaagaa aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct
      781 acttgaattg atccattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga
      841 agcggagcgt tcaggggata ttcgtgattc cttagcggat atttcaaaaa ttcgttcttt
      901 aggctatcag cctaaatttg atatattgag cggaatggaa cggtatttaa agacagaaat
      961 ttgatgaaaa aatgattgca tctttgg
//
LOCUS       DAXWLH010000345          974 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXWLH010000345 DAXWLH010000000
VERSION     DAXWLH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.353"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            109..963
                     /locus_tag="ACGE36_002924"
                     /pseudo
     CDS             109..963
                     /locus_tag="ACGE36_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          348 a          151 c          189 g          286 t
ORIGIN      
        1 aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt
       61 ctgtaaaatt aaagttacca cacagaaatt ttataggagg actttctcat gactaaattt
      121 actatagaaa ttatgcaaac actactcaat aaaggggatt tagatgaatt atttcaggat
      181 catttagagc gagcgattaa ttcacttcta caagcagagc taactgcttt tttagattac
      241 gaaaaatatg atcgaaatgg attcaattca ggaaattccc gtaacgggaa ttattcacgt
      301 acatttaaaa cagaatatgg cgagttgaat ttaactattc ctagagatcg gaatggcgaa
      361 ttttctcaac aaacactacc agcatataag cgaaccaatg attcattaga aacgaccatt
      421 atccaattat ttcaaaaagg cattactatg gctgaaatct ccaaattaat tgaaaaaatg
      481 tatggtcacc actatacgcc acaaacaatc tccaacatga gtaaattggt ggctgaagat
      541 gttttagctt ttaaagaaag aacgttagaa gccaattatt ccgttatatt tatggatgcg
      601 acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag cagtgtatat tacgattggt
      661 attcgtttgg atggaaccaa agaagttctt gggtttacta ttgccccaac tgagtctgct
      721 tatatttgga aagaagttct tcaagatctt agaaaacgtg ggttagaaga agttttatta
      781 gtagtgacag acggattaag ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc
      841 caatttcaac aatgttgtgt gcatgtatct agaaatatcg ctcataaagt tcgtgttcga
      901 ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc aagcgaattc aaaagaagag
      961 gcgtaagcgc ccca
//
LOCUS       DAXWLH010000346          964 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXWLH010000346 DAXWLH010000000
VERSION     DAXWLH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.354"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            81..773
                     /locus_tag="ACGE36_002925"
     CDS             81..773
                     /locus_tag="ACGE36_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8385224.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          422 a          146 c          162 g          234 t
ORIGIN      
        1 gtaatttaaa aacgcttgac attgtgtttg agatcactta cttttagcat gtaaaagtat
       61 tggattaagg agctatgcaa atgaaaaaga aatttattac tggtgggatt gtcgttgcat
      121 ttctatgtct aattggctac acaggcacgc ggtacgcaaa tgaaattatt ttgtggggtg
      181 gagagacaga tattgataca atcaatgaga atctgacaca gcttgatcat acattgacag
      241 ataaagagca agcgattaca gatcttaccg ctcgactagc acaaaaagaa cagacattgc
      301 aagattcaaa ggcagatgtc caagaatacc aaacaaaagt aaatgaatta gaaaaccaaa
      361 aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca attagtcagc aaggatgcac
      421 aactacaaag caaacaaaat gaaatcaatg tcaaactaga tgagatcaac caaaaaaatg
      481 atgaaatcaa tcatctgaat gcagattgga cagcaagact acaagaagca caagcaaaac
      541 tttcagaatt acaaaatact gtaaatggtt tgaatcaaca aatcgcaaca ctgacacaag
      601 aaaaaacaaa tgtagaaact cagttaacag atacacaaaa gaaattagac gaagcaactg
      661 ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa gatgaagtgg
      721 aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa taatctttct
      781 taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactaagt agttcagttt
      841 tggagtacaa aatgacaaaa tgtacttcaa aatggactac ttttttcatt ttaacttaca
      901 taccaaatta catacaaaat gatttataaa atgcaattga aaatgataat tgaatgaaaa
      961 tttg
//
LOCUS       DAXWLH010000347          962 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXWLH010000347 DAXWLH010000000
VERSION     DAXWLH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.355"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(61..285)
                     /locus_tag="ACGE36_002926"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACGE36_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACGE36_002927"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACGE36_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          328 a          166 c          149 g          319 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 ag
//
LOCUS       DAXWLH010000348          958 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.356, whole genome shotgun sequence.
ACCESSION   DAXWLH010000348 DAXWLH010000000
VERSION     DAXWLH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.356"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..393
                     /locus_tag="ACGE36_002928"
     CDS             <1..393
                     /locus_tag="ACGE36_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8385225.1"
                     /translation="FLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYD
                     SLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLV
                     HGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            623..853
                     /locus_tag="ACGE36_002929"
     CDS             623..853
                     /locus_tag="ACGE36_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFF8385226.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          326 a          151 c          155 g          326 t
ORIGIN      
        1 tttcttcgcg taggagttca agcaaaagct gtactagacc ctcattttca agcccaagtc
       61 gcttctttat taacagttaa tgatttagta atcatttttt ctttatctgg gaaaacgaaa
      121 gatacttatg attcattgaa aatagctaaa aaaaatggcg caaaaattat tgctataact
      181 aactatattc attcacctat aggaaagtct gcagacttag ttttacaaac agccattgaa
      241 gaatttctta atggtggatc tttagctgga aaaatatcac aactttatat ttgtgatcta
      301 ttagttcatg gttatgaaca aaacaataat attgattcag ttgaattaag agaaaaagtt
      361 ttgcgctcta ttattgataa aagtattgag tgaactaccc ccacttagct aaacctaacg
      421 gtttttaacg attgaagtag gcaattcttg ggatgagagc atacttgata gcttattttt
      481 tactaatagc ggtggctctt attgaccaaa tatccctgta gttcctacgg ttcttgattt
      541 tatctaaaga tatttttatc gtggaagcta ttgatcaact tgaaagaaaa acgtgtagct
      601 cattatcact agtttatcga gtatggatga agcaatggat catcctctct taaataaatt
      661 tattaaggat ctgatccttc aaattttttc catgatcgat gagcaggaac ctactgaatc
      721 taaacgacat cgggcacaag gaattattaa gattgctaag cgaatgtttt ctataaaggg
      781 tgttctaagc tatacagtgt taattcaaaa gattcccaat catcttgttt ataagaacaa
      841 cgttgaagat taaaaaagtg tttcaatgct aaaattgcaa aatacttcaa tgttatgaga
      901 caaactgtct ataagaatca atttccaccc atttcatcac ttgttaaaag tttatgat
//
LOCUS       DAXWLH010000349          956 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXWLH010000349 DAXWLH010000000
VERSION     DAXWLH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.357"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..157
                     /locus_tag="ACGE36_002930"
     CDS             <1..157
                     /locus_tag="ACGE36_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialic acid O-acetyltransferase NeuD family
                     sugar O-acyltransferase"
                     /protein_id="HFF8385227.1"
                     /translation="DKNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPT
                     KVIERWD"
     gene            159..>956
                     /locus_tag="ACGE36_002931"
     CDS             159..>956
                     /locus_tag="ACGE36_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFF8385228.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKK"
BASE COUNT          355 a          125 c          206 g          270 t
ORIGIN      
        1 agataaaaat gaaattggag taggtactaa aataatacaa aatctaagaa ttggaaatat
       61 gaacattata ggtgcgggaa gtgtagtaat aaaaaatatt aataataaga ctaaaaatgt
      121 tggcgtacct accaaagtca tagaaagatg ggattaagtt ggaaaataaa cgaatattat
      181 tagcatctcc tcatatgagt gatgaaggat atgaacaaga gtatgtaaaa gaagcatttg
      241 atacaaattg ggtggctcct ttgggtatga atgtcaatgg ttttgaagag gaattagcag
      301 aatatgtcgg tgctagtcat gcagctgcac tatcttctgg aacagcagct atacatctag
      361 ctttaaaagc ggctggggtc ggtaaagatg atatagtgat ttgtcagtct ttgacgttct
      421 cagctactgc caatccaatc atctatcaag gagctattcc agtatttgtt gatagtgatt
      481 acgaaacttg gaatatgaac cctgaacatt tgcgaacagc gctagaaaaa tatcctaacg
      541 caaaagctgt attagtagtg catttgtacg gtttatcagc tgaactagat gaaattacta
      601 aaatatgcaa agaacataat gttgttttaa ttgaagatgc tgcagaatca ttaggaacaa
      661 catataaagg aaaatatact ggtacattcg gcgactatgg aatttattct tttaatggaa
      721 ataagataat cacgacttca ggtggcggaa tgcttgtgtc taatgacaaa gaaaaggtgg
      781 aaaaagtgag gttttgggct acgcaatctc gtgagaaggc acgccattat caacacaatg
      841 aaattggcta taattatcga atgagcaata tcgttgcagg tatcggtcga ggacaattga
      901 aagttttaaa tgaaagaatt gagaaaaaac gatatattca tagtttttat aaaaaa
//
LOCUS       DAXWLH010000350          949 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXWLH010000350 DAXWLH010000000
VERSION     DAXWLH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.358"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..480
                     /locus_tag="ACGE36_002932"
     CDS             <1..480
                     /locus_tag="ACGE36_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HFF8385229.1"
                     /translation="KGIKIICRQGTVLFLLSLYEHEIHFQNYYNTTTKLGAFLLLYLV
                     SNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVELFLFKMDSKWDKYIYRSKEV
                     FLTGSYNETLQESLYDTGAIFNNEPMVLKDFFGDYELVWNPKQLSEQGAEDEFAEVH"
BASE COUNT          366 a          126 c          160 g          297 t
ORIGIN      
        1 aaaggaataa aaatcatttg tagacagggc acagttttat ttctacttag cctgtatgag
       61 catgaaattc attttcaaaa ttattataat acgacaacta aacttggtgc atttttactc
      121 ctatatttag ttagtaatat agctgaaaaa atgctgttta aacatattgt aaagcaagtc
      181 ttttctgata ttcaaaaaga aagctattgt ttaaaaacac cttatggaaa tttaaaaaat
      241 gttgagctgt ttttatttaa aatggatagt aagtgggata aatatatcta tagaagtaaa
      301 gaagtatttt taactggtag ttataacgaa actcttcagg agagcctgta cgacacagga
      361 gcaatattta ataatgaacc tatggttttg aaagatttct ttggagatta tgaactagtt
      421 tggaatccaa aacagcttag tgaacagggg gcagaagatg aatttgctga agttcactaa
      481 catagtgtac agaaaaacat tatctgaaag aagaacgcct tcaaaagaaa gtataagaac
      541 gattgggccg tacagaagac taacgaaaga tcgtaaaaaa aaggaaagac aatgaaaatg
      601 tctttccttt tttttgacta caagtagcct gaaaatgaaa ttccaaaaga ctacaagtag
      661 tcttttgtgg caaaaataaa gagccaacga aagctaccaa caatcgttga ctcgtaagac
      721 tgttcgtagt aagaaaaatc tttggcaatc cacgaagtct ttatttcata atttatataa
      781 atgattatac agtcattttc ctgaatttgc aagtaaaaaa gctaagaaat attgtttaag
      841 cgtttcctta aaagaatctt taaataaatt tttttccaat tgaaaacatg actacaagta
      901 gactatttct tttattccaa gttgactact tgtagtcaag gtgatataa
//
LOCUS       DAXWLH010000351          935 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXWLH010000351 DAXWLH010000000
VERSION     DAXWLH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.360"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..797)
                     /locus_tag="ACGE36_002933"
     CDS             complement(<1..797)
                     /locus_tag="ACGE36_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385230.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKI"
BASE COUNT          281 a          185 c          147 g          322 t
ORIGIN      
        1 ttaattttag caaaagctag tgcatcttga atcaattcga gcacttctgc cttcgactta
       61 tttttagcct gtgcgttctt cgctaattgg gcagcagctc gtgctaagta ataagtcaaa
      121 tggttatccg aacaaatatc gattgcgtaa tttagtagtg catcacttgt ttccaaatca
      181 ccgtcttgcg caaaaaaaac accacaatga aagacgatat ttaacacccg ccagatatct
      241 tctgttgtac gaatcggata cgtataaatt ttttcaaaca ctcgattgaa ataaacttct
      301 gctttttcca actctttttc tctagcatag accattccaa tacctgtata tgccaataaa
      361 cgaaacaacg tgttggctgt ttctgccatc aacaactgat caaaatggaa caacacatcc
      421 gtaattggtg cattcgtaaa aatcatttca aatcctttta gatagtagta acgtgcaatc
      481 agctcagact cgtcaattac cttgggatca atggctgcta aaatctgtgt caattgttca
      541 tattcactcg taatcagtga aaactctgct ttttctagct gttcatcaag ttgtgtattg
      601 atcacgttca tcgaagaaaa aagttcaact aacggaagat tgattcgatt acataatttg
      661 attaaaattt ttaacgaagg gacttgacca ttgttctcaa atttacttaa tgttgcttgt
      721 gtacaaattc cttcagacac ttctttttga gataagttgg cagcctttct tgccgtaata
      781 aatttttcaa tgttcattat tttctctcca atcagacctc acacgaaaat catttctgtc
      841 tcgattttag catgttttct ttgggaatgc tttaggttct gttgcaaagt tttaaataaa
      901 gaataaagtc ccttacggta tctatgattt aagct
//
LOCUS       DAXWLH010000352          903 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.361, whole genome shotgun sequence.
ACCESSION   DAXWLH010000352 DAXWLH010000000
VERSION     DAXWLH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.361"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..>903)
                     /locus_tag="ACGE36_002934"
     rRNA            complement(<1..>903)
                     /locus_tag="ACGE36_002934"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          193 a          277 c          203 g          230 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 ggg
//
LOCUS       DAXWLH010000353          892 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXWLH010000353 DAXWLH010000000
VERSION     DAXWLH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.362"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(1..99)
                     /locus_tag="ACGE36_002935"
                     /pseudo
     CDS             complement(1..99)
                     /locus_tag="ACGE36_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            178..876
                     /locus_tag="ACGE36_002936"
                     /pseudo
     CDS             178..876
                     /locus_tag="ACGE36_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          312 a          159 c          172 g          249 t
ORIGIN      
        1 aatatgggca tagacttgtg tagtggaaac atctttatga cctaaaaaat ctttgatatc
       61 ttctaaaggt attcctgctt gtctcgttct agctgaaaaa gcaaaacata atgttggtgt
      121 tttcctttca gcatatctat cagcaagatg tagagtagga caacctcgtg agccaaaagt
      181 cctgtgcaaa tcgagttaca acacacacat gataaagaaa cttttgaaaa gaactataaa
      241 gtatacgttg aattggcaag agattcagca atgaaatatg gtattccatt aacattagac
      301 actccttata accaaccagg aatcaaatcg catttatggg taacacaaaa tatttggggc
      361 gatcatacag atccttacgg ttatctttct gaaatgggtg taagtaaaga aaaactagcc
      421 tatgatttgg ctcatggttt tacggatgat aatccaacta cttcggagga taaaccagta
      481 attgatccaa ctcgagcagg tgctgcaaat tctacgctga cagatggaac aaattacgcc
      541 cacattgatc agttcggaga aatcgaaaac gcaaacttgc atgtagctgg atggcacatt
      601 gctaattata aatacgagta tattttcatt atggactaca atactggaaa agaactagct
      661 agagtaagag ctgatgggat ttatagacca gacgtaaatc aagcttataa tactttagga
      721 aatgttggct atcatgtatc tttcaatatg cgtgatttcc ctaataagaa agtatacgtc
      781 atgatgcgtg cgacaaacga tccagaagga aatactaagg gtggagcaca agattttcat
      841 gacaagcgtt ggtatttaaa tattccacaa cgataaaaaa tccccccctc tt
//
LOCUS       DAXWLH010000354          878 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXWLH010000354 DAXWLH010000000
VERSION     DAXWLH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.363"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..602)
                     /locus_tag="ACGE36_002937"
     CDS             complement(<1..602)
                     /locus_tag="ACGE36_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8385231.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          277 a          160 c          176 g          265 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaattt gatcaaagtg gtagctgtta ttgaattcaa gaattgtttc tcgtatgaat
      481 gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa ttgattgtcg
      541 tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac aaataatttc
      601 atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac tgtgggattt
      661 cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat aaaagagcta
      721 gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca gctaatgagt
      781 tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac gctacaggag
      841 gaaatagcaa attcccgttg gatcctttcc atacatcc
//
LOCUS       DAXWLH010000355          877 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXWLH010000355 DAXWLH010000000
VERSION     DAXWLH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.364"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(247..468)
                     /locus_tag="ACGE36_002938"
     CDS             complement(247..468)
                     /locus_tag="ACGE36_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385232.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..868)
                     /locus_tag="ACGE36_002939"
     CDS             complement(566..868)
                     /locus_tag="ACGE36_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385233.1"
                     /translation="MKRKDKYYRLLLLTAEAGWMDFPEVVKEVQEIGATISDSRSSPM
                     NYQDAHGIKIIDQERNVRKYTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          264 a          167 c          112 g          334 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaa
//
LOCUS       DAXWLH010000356          874 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.365, whole genome shotgun sequence.
ACCESSION   DAXWLH010000356 DAXWLH010000000
VERSION     DAXWLH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.365"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..353)
                     /locus_tag="ACGE36_002940"
     CDS             complement(<1..353)
                     /locus_tag="ACGE36_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8385234.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQ"
     gene            complement(371..691)
                     /locus_tag="ACGE36_002941"
     CDS             complement(371..691)
                     /locus_tag="ACGE36_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8385235.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          291 a          154 c          116 g          313 t
ORIGIN      
        1 ttctgaatag aataatactc cataaatttt tcgagattaa actgagattc atcagtcagt
       61 tcgttctcaa tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca
      121 aattttgagt gaaaagttgt agaagtatcg tacaaaactg aatattcaaa atgagaagcg
      181 gtgaaatgcg caagttcatg agataaatga aaggcttctg cagtttcgcc atatagattt
      241 tcattcaaaa aaatgattct aggcttcgga taatagaagc caggctcttt catttccatg
      301 taaactactt tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaccc
      361 atcactccaa ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg
      421 tctccttaga tatttctttt ccatcaaaag aaaaaacagt atcgttttct gataaatcca
      481 catgtttagg ggtttctttt tcttcgcgcc ccagaagata gtctacagag acatcgaaat
      541 agtcagcaat ttcttgcaaa cgttctgtag aggggtttga gttcttaagt ctatataaga
      601 cgtttttacc atagcctagg tcttcttcaa ctttatttaa tgattttccc tgttttttcg
      661 ctaactcttt tattctctca aacgttatca tatcaatatt cctttcgttc aagaaaaaat
      721 atttaacttt atttgttaaa attcgttgac tattttaaca aaaggtgtta atatcattct
      781 tgtaaacaag tttaacaact aaaaagacaa caaaaaacac tattgattaa taaatgccaa
      841 ccgccaagaa agcttttaaa atcaatgttt atat
//
LOCUS       DAXWLH010000357          863 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXWLH010000357 DAXWLH010000000
VERSION     DAXWLH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.366"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..861
                     /locus_tag="ACGE36_002942"
     CDS             <1..861
                     /locus_tag="ACGE36_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFF8385236.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DAXWLH010000358          844 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXWLH010000358 DAXWLH010000000
VERSION     DAXWLH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.368"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            76..690
                     /locus_tag="ACGE36_002943"
                     /pseudo
     CDS             76..690
                     /locus_tag="ACGE36_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          309 a          133 c          151 g          251 t
ORIGIN      
        1 taacattgaa aaatttagag ttctaacgta tgattagggt gtattataaa cgttatagaa
       61 aagtagggaa taaatttgaa aattgcttat attagagttt catccatcga tcaaaatgaa
      121 caacgtcaaa ttgaagaaat gaaaaagttt ggcgctgaaa aaatttttat cgaaaaacaa
      181 tctggagcga cgattagtca tcggccgatt ttccaggaag ctttagattt tgtcagagag
      241 caagatattt ttattgtaga agccattgat cgacttggac gcaattatga cgagattatc
      301 gattcagtga attatctaaa gaagaagaat gtgcgactta ttatcaccag tttaccgatc
      361 atggctgaag caattggaaa tcctctccta gataaattca tcaaagactt aatcatttaa
      421 atctcagcca tgattgctga acaagaacgt actgaatcga aacgacgcca ggcacaagga
      481 attaaaatcg ctaaagcaaa cggcgtctat aaaggtcgtc ctaagttata cagtgcagac
      541 accaaagatc ctcaacgtcg ccttgtctat aggagtatcg ttcaggatct tgagaatggg
      601 gttgctatct caaagattgc aacagactac aatgtgacta ggcaaacaat ttaccgaata
      661 aaaaaagaaa tcgaccaact aattgtatga tttctttttt gaatcacgta attattaatt
      721 agctttgtca tcaatacaga caatcatttt agattcatct agctaagttg attacattta
      781 tgttaaaaag aatttagtta acataaatat aattgatgca taaatcgtcc ttatttaaag
      841 gcat
//
LOCUS       DAXWLH010000359          841 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.369, whole genome shotgun sequence.
ACCESSION   DAXWLH010000359 DAXWLH010000000
VERSION     DAXWLH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.369"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>841
                     /gene="bsh"
                     /locus_tag="ACGE36_002944"
     CDS             <1..>841
                     /gene="bsh"
                     /locus_tag="ACGE36_002944"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8385237.1"
                     /translation="TSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVNDLD
                     THYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPW
                     ILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYD
                     NPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSS
                     VSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCN
                     VDKG"
BASE COUNT          283 a          115 c          155 g          288 t
ORIGIN      
        1 gtacgtctat tacttatgta acaagtgatc attattttgg aaggaatttt gattatgaaa
       61 tatcttacaa tgaagtagtc actattactc caagaaatta taagttgaat tttcgaaagg
      121 taaatgattt ggatactcat tatgcaatga ttggtattgc cgctggtata gctgactacc
      181 ctctttatta cgatgcgaca aatgaaaaag gattgagtat ggctggacta aatttttctg
      241 ggtatgctga ttataaagaa atacaagaag ggaaagacaa tgtatctcct tttgaattta
      301 ttccttggat tttaggacaa tgctcaacag taggagaagc taaaaaattg ttaaaaaata
      361 tcaatttagc aaatataaat tatagtgacg aacttccttt atccccttta cattggctat
      421 tagctgataa agaaaaatca attgtcattg aaagtatgaa agatggactt catatatatg
      481 ataaccctgt gggcgttctt accaataatc cttcatttga ctatcaatta tttaatttaa
      541 acaattatcg tgtcttatcg agtgaaactc ctaaaaataa tttttcaaat caaataagtt
      601 tgaatgccta tagccgcggt atgggaggga taggcttgcc tggagattta tcctcagtat
      661 ctcgttttgt taaagcgact tttacgaagc tgaattctgt atctggagat tcagagtcag
      721 aaagtattag tcaatttttc catatcttag gttcagtaga acaacaaaaa ggtttgtgtg
      781 atgttggtga tggaaaatat gaatatacaa tttattcttc ttgttgcaat gttgacaaag
      841 g
//
LOCUS       DAXWLH010000360          836 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXWLH010000360 DAXWLH010000000
VERSION     DAXWLH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.370"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..697
                     /locus_tag="ACGE36_002945"
     CDS             <1..697
                     /locus_tag="ACGE36_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8385238.1"
                     /translation="SATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAIL
                     SDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHE
                     KVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQY
                     KADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINI
                     LTEGLRLHSMGLA"
BASE COUNT          305 a          133 c          154 g          244 t
ORIGIN      
        1 atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt tgtgtaaaga
       61 agaaattatc gaattaccta aaactaattc cacaattggt attgacttag gtattatgga
      121 ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt ccaaaatgga
      181 aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg ctaaaaataa
      241 aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag tagctagatt
      301 gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta cagaaatgat
      361 caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt tgcgaaatca
      421 taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta aattacaata
      481 caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc cgtctagtca
      541 gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc gagaatggac
      601 ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca atattctgac
      661 cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa gaaccgtagg
      721 aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa tacgctatca
      781 agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca ggttta
//
LOCUS       DAXWLH010000361          830 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXWLH010000361 DAXWLH010000000
VERSION     DAXWLH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.371"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..830
                     /locus_tag="ACGE36_002946"
     rRNA            <1..830
                     /locus_tag="ACGE36_002946"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          204 a          178 c          262 g          186 t
ORIGIN      
        1 catggagctt tactgtagtt tgatattgag tgtctgtacc gcatgtacag gataggtagg
       61 agccgtagaa atcggaacgc tagtttcgat ggaggcgctg gtgggatact acccctgcgt
      121 tatggccact ctaacccgca ccactaatcg tggtgggaga cagtgtcaga tgggcagttt
      181 gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt
      241 tggaaatcat tcgaagagtg taaaggcaga agggagcttg actgcgagac caacaagtcg
      301 agcagggacg aaagtcgggc ttagtgatcc ggtggttccg catggaaggg ccatcgctca
      361 acggataaaa gctaccctgg ggataacagg cttatctccc ccaagagtcc acatcgacgg
      421 ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg
      481 ttgggctgtt cgcccattaa agcggcacgc gagctgggtt cagaacgtcg tgagacagtt
      541 cggtccctat ccgtcgcggg cgttggaaat ttgagaggag ctgtccttag tacgagagga
      601 ccgggatgga cttaccgctg gtgtaccagt tgttctgcca agggcattgc tgggtagcta
      661 tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa gcccacctca agatgagatt
      721 tcccatttct ttaagaaagt aagatccctg agagatgatc aggtagatag gtcaggagtg
      781 gaagtacagt gatgtatgga gcggactgat actaatcgat cgaggactta
//
LOCUS       DAXWLH010000362          809 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.372, whole genome shotgun sequence.
ACCESSION   DAXWLH010000362 DAXWLH010000000
VERSION     DAXWLH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.372"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..721
                     /gene="aph(2'')-I"
                     /locus_tag="ACGE36_002947"
     CDS             <1..721
                     /gene="aph(2'')-I"
                     /locus_tag="ACGE36_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFF8385239.1"
                     /translation="KAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTP
                     EIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYND
                     LTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGII
                     DEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIK
                     NIKQEFIENGRKEIYKRTYKD"
BASE COUNT          354 a           54 c          130 g          271 t
ORIGIN      
        1 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
       61 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      121 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      181 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      241 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      301 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      361 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      421 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      481 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      541 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
      601 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
      661 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
      721 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
      781 gaaaaataat aaaggaagtg agtcaattc
//
LOCUS       DAXWLH010000363          793 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXWLH010000363 DAXWLH010000000
VERSION     DAXWLH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.373"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..130
                     /locus_tag="ACGE36_002948"
     CDS             <1..130
                     /locus_tag="ACGE36_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8385240.1"
                     /translation="TASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
     gene            270..>793
                     /locus_tag="ACGE36_002949"
     CDS             270..>793
                     /locus_tag="ACGE36_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385241.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCG"
BASE COUNT          279 a          118 c          160 g          236 t
ORIGIN      
        1 tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg
       61 aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat taatgggaat
      121 aacagcctaa gatatttgga gttcagagag ggcgcgtttg attttcaaac ttcgcaacag
      181 aaccgagaac cttgggtttt caagtgcttt ttggttcatt aatcttaacc atagtgtttt
      241 taaagtggaa aacaagcaac aaaaaaagta tgtggagcct tgtaggaata gttcttatta
      301 tttttactgt gactgcagca acagaagcaa attattcgca taaaattcag tcgtataaag
      361 gtcgtttagt tatgaccaaa aaagatgtta cacgttatac atcctcaaga actggaggcc
      421 tatatgacta tgcaccatta gatgcattga aatataaaga tcatttaaag aaacatgaag
      481 taaaagttgg tgatcggtgg gagaatgcat ctcataaagc atataattcc aaaatagtct
      541 atacagtaga agatgccgat gagcaaaata tcacattacc tgtattcaat tattggggaa
      601 cagttgtgaa agtaaatggg aaaaagagca ctatagaaaa tgacaatggt ttagtgtcta
      661 ttaaaggtac ggggaaaaga actatagttg aggtttcatc tacgtatcca actacgatga
      721 ttctatcttt attaattagt tcaataactt ttatattctt atttacacgt tatttaataa
      781 gatgctgcgg tga
//
LOCUS       DAXWLH010000364          779 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.374, whole genome shotgun sequence.
ACCESSION   DAXWLH010000364 DAXWLH010000000
VERSION     DAXWLH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.374"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..579
                     /locus_tag="ACGE36_002950"
     CDS             <1..579
                     /locus_tag="ACGE36_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFF8385242.1"
                     /translation="TSDYLEKTVNQNKFDIGSYQVEEKKKVYSILNINSGEQFVIFKN
                     ISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDPNVMKVVMEFEKNSPK
                     GDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKTLSSKTVKELLENGKT
                     KEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
BASE COUNT          343 a           73 c          130 g          233 t
ORIGIN      
        1 acttcggatt atttagaaaa gactgtaaat caaaataaat ttgatatagg tagttatcaa
       61 gtggaagaaa agaaaaaagt ttattctatt ttaaatatta atagtggtga acagttcgtg
      121 atatttaaaa atatatcagg aaaagcaatc aataaaaaag ttcttcaaga actattacta
      181 aataatcgta cagataaagt cctttccggc tttaagtcaa aagaaggaaa agcttttaat
      241 gcaagattac ttttcgatcc aaatgtgatg aaagtggtaa tggagtttga aaagaacagt
      301 ccaaaaggcg ataaaaagag caaacacgat acagtaaatg gttatgaaat agttgaaaaa
      361 gcaaaagtat ttgaaataaa agaattagca acaggtgaaa gttttatttt ctataaaaat
      421 aatagtggga aaactttaag ttcaaaaaca gtcaaagaat tgttagaaaa tgggaaaact
      481 aaagaaaaag taacgggttt tataagtaaa gacaataaaa aatatagtgc ttatcttgtc
      541 tttgataata aaaacaaagt tataaagaaa gaattttaat tcttagtggc ggaaaacatt
      601 tgtttttccg cttattttta ttcaaaagta cccaaaatat aggtatactg aattaaggat
      661 aaatacaaaa ctagttagag gacagttaaa aaaatacaaa caaattagat tgctattttg
      721 ttaaattgga agtagaataa taccgttttg atatgtatag tgtgttttac caataaaat
//
LOCUS       DAXWLH010000365          773 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.375, whole genome shotgun sequence.
ACCESSION   DAXWLH010000365 DAXWLH010000000
VERSION     DAXWLH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.375"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE36_002951"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE36_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFF8385243.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACGE36_002952"
     CDS             complement(481..675)
                     /locus_tag="ACGE36_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385244.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          229 a          163 c          134 g          247 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaactta tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt aaaaatgttt tgc
//
LOCUS       DAXWLH010000366          766 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXWLH010000366 DAXWLH010000000
VERSION     DAXWLH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.376"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(97..495)
                     /locus_tag="ACGE36_002953"
     CDS             complement(97..495)
                     /locus_tag="ACGE36_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFF8385245.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(492..728)
                     /locus_tag="ACGE36_002954"
     CDS             complement(492..728)
                     /locus_tag="ACGE36_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8385246.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          239 a          134 c          118 g          275 t
ORIGIN      
        1 ttatgaaaaa actattttgt atgattaaac ataaaaaaat cagcatattc cttaatgaca
       61 tgctgatcta attgagaaat aaagttttta attcgttcat tttattgcgg tttgtcgtaa
      121 ataactggtt atcttggact ttagtgaatc gaaatccaat gaactagtgg taatcattat
      181 gataaaatca acagcttctt gacgagaaaa agcaactgga caattattca attgaagaaa
      241 caacaacatg gcaacaagag cagttcgttt attgccatta tgaaaaggat gttttttagc
      301 taaattaatt gctaaaatcg ccgctttttc atagatttca ggatagagtt cttgattgaa
      361 tactgcttgt gaaggctgtt tgacggccat atctaatgca tttgcatctt ttacaccgat
      421 catttcgcct ggcgagtgac gtaaaataac tttcgcgttg attttgataa tttctttagt
      481 cgaaagatac ctcataaatc aaccaaatct tttaacgcgt cttcatattg attcatgatc
      541 gaatcaacta atgaatcaat atcaggttct tcttgtgctt tgatcattcg tacagaacca
      601 tcagattgaa aaacataact gattgcgtca cctgtctgca cacccatgaa ttcttttact
      661 tctggtggga tagttgttac atcgctattg ccagattttc taattttata ctttttcatt
      721 tttttcatat ttcgccaccc tctataccat tctcattcta tagtaa
//
LOCUS       DAXWLH010000367          744 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXWLH010000367 DAXWLH010000000
VERSION     DAXWLH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.377"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            30..>744
                     /locus_tag="ACGE36_002955"
     CDS             30..>744
                     /locus_tag="ACGE36_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFF8385247.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELN"
BASE COUNT          289 a           90 c          124 g          241 t
ORIGIN      
        1 tgattaagat agaatctgtg aggtaagaga tgaagaatat aaaaaaattt ttttcaagga
       61 atattttccc ggtttttatt aaaatattac ctattcaaaa aaatttaatt gtattttcaa
      121 gttttggcgg gcgtaattat tctggaaatc caagagttat ttcagagaaa attcatgaaa
      181 aaaaattgaa ttacagaatt atttggttaa tcaaaaaaga agcgattata actgatttac
      241 cagattatat taaaagaatt gattccgatt cttttttagc tgccttttat ttgtatacag
      301 ctaagtattg gatagacgat agtcggaaaa cgattaagta tgcaaaaagg aaaaaacaat
      361 tttattttca aacatggcac ggtacgcctt taaaaaaaat tgaatttgat gcaaaagata
      421 agttacctaa acgttaccta agctatgcga aaaaagatag tgagtcaatt acatatttat
      481 tatctggtaa tcgctatagt tctgagaagt atgtttcagc attcaatatc gttccttcta
      541 aaatattaga agtaggaact cctagaaacg atgaattagc ctctagtaaa gagcaagtgt
      601 ttgacttgaa aaagaaacaa atcaatgtct tatttgctcc aacttttcga aataatattg
      661 aggacaacgg aattacgcaa ctagaatggt taggtgtgga taacttacgt gaatttttta
      721 aaaagcaaca acaagaatta aatt
//
LOCUS       DAXWLH010000368          721 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.378, whole genome shotgun sequence.
ACCESSION   DAXWLH010000368 DAXWLH010000000
VERSION     DAXWLH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.378"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>721
                     /locus_tag="ACGE36_002956"
     CDS             <1..>721
                     /locus_tag="ACGE36_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFF8385248.1"
                     /translation="KGAYNLGDGKYLYSIYKDQDSSEWLGYLKAHVSLAYTPIEEINK
                     NVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARLKLTNVSNNAVYYKLYKSGKFYGYIN
                     AEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNYSKGKTERGRVFYISISYNTADNQPI
                     YSVYTDETCTEWRGYYKGNNFEDTQITMLENKSVKVTKSGYTVWGDLNFWTKSGISNT
                     GDIYTTDRKFYNFTNNAYYYEL"
BASE COUNT          273 a           92 c          133 g          223 t
ORIGIN      
        1 aaaggagcat ataatttagg ggatggaaag tatctatatt ctatttataa agatcaggat
       61 tcctctgaat ggttagggta tttaaaagca catgttagtt tagcctatac tcctattgaa
      121 gaaattaata aaaacgtaac tgttactaaa aattggtcga tttggaataa tctacaatgg
      181 gaaaaggaaa ttgaaaagcc tcagattggt tctgtatttt cagctagatt aaagttaacc
      241 aatgtatcga ataatgctgt ctattacaag ctttataaat ctggaaaatt ttatggttat
      301 atcaatgctg aggctgtcaa agaccttaca acaacaaagc ttaataagta tgttacattt
      361 agtgttaata atgaagattt ttggtcttct ttagatgtaa attattcaaa aggaaaaact
      421 gaaagaggcc gagtatttta cattagtatt tcatataata ccgcagataa ccaaccgatt
      481 tattcagtat atacagatga aacatgtacg gaatggcgag ggtattataa gggaaacaac
      541 tttgaagaca ctcaaattac tatgcttgag aataaaagcg tgaaagtaac aaaaagtggg
      601 tatacagtat ggggagactt gaatttttgg actaagtcag gaatatcgaa cacaggagat
      661 atatacacca ctgaccgaaa attttataat tttaccaaca atgcttacta ttatgaattg
      721 a
//
LOCUS       DAXWLH010000369          714 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.379, whole genome shotgun sequence.
ACCESSION   DAXWLH010000369 DAXWLH010000000
VERSION     DAXWLH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.379"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            38..>714
                     /locus_tag="ACGE36_002957"
     CDS             38..>714
                     /locus_tag="ACGE36_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFF8385249.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLA"
BASE COUNT          239 a          139 c          130 g          206 t
ORIGIN      
        1 ctaaatataa ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt
       61 tcctgaaaac gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt
      121 gaactataca agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt
      181 gatcacttgg gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa
      241 aaaatgcaaa cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc
      301 caactactca ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg
      361 ggagctttta acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa
      421 gatgccattt catgcaataa attattcaga taaattaaca actatcatcc ataaatacga
      481 tcatattatc aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa
      541 gttcatccaa gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga
      601 attgatcaat gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg
      661 aacccttctt ctgatagagg actgttatta tttatggaaa gaattattag caga
//
LOCUS       DAXWLH010000370          712 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.380, whole genome shotgun sequence.
ACCESSION   DAXWLH010000370 DAXWLH010000000
VERSION     DAXWLH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.380"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            41..364
                     /locus_tag="ACGE36_002958"
     CDS             41..364
                     /locus_tag="ACGE36_002958"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFF8385250.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            396..712
                     /locus_tag="ACGE36_002959"
                     /pseudo
     CDS             396..712
                     /locus_tag="ACGE36_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
BASE COUNT          259 a           94 c          134 g          225 t
ORIGIN      
        1 ataatttttt gttaatgaac aagataggaa gaaacattta atggacagtt ttcagagatt
       61 gtcactagta aatcaatttg agttattggc taaatttgag gaccaaaaca gatcttatta
      121 tgaaagaaaa atcgaaatat tgagagaagg ttacgagtat cattatgatg atgaaatttg
      181 gagtgatctt tctgagccat ttccgaaaga agattctcga tttgtattag atgtattgaa
      241 catgtatcga gatatcaatt ttagtaaatc aaagctaaac ttagatgaaa accaaattac
      301 taattcatac tatactcatt tcggttctgt tgcaaagttt tccaaaaaat ctattttagt
      361 gtaaaattga gaaaaaagac agagaggaca gagtaatgaa tcattttaaa ggcaaacaat
      421 tcaaaaaaga cgtcattatt gtcgctgttg gttactacct gcgttacaat ctaagctatc
      481 gtgaagttca ggaattgtga tatgatcgtg gaataaatgt ttgtcatact acgatttatc
      541 gttgggtaca agagtacagc aaagtcctct attatctttg gaagaagaaa aatagacaat
      601 ccttctattc atggaaaatg gacgaaacct atatcaaaat taagggacgt tggcattatc
      661 tttatcgtgc aattgatgcg gacggcttaa ccttagatat ctggttacga aa
//
LOCUS       DAXWLH010000371          697 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.382, whole genome shotgun sequence.
ACCESSION   DAXWLH010000371 DAXWLH010000000
VERSION     DAXWLH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.382"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>697
                     /locus_tag="ACGE36_002960"
     CDS             <1..>697
                     /locus_tag="ACGE36_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFF8385251.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDL"
BASE COUNT          275 a           92 c          120 g          210 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttg
//
LOCUS       DAXWLH010000372          695 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.383, whole genome shotgun sequence.
ACCESSION   DAXWLH010000372 DAXWLH010000000
VERSION     DAXWLH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.383"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(53..628)
                     /locus_tag="ACGE36_002961"
     CDS             complement(53..628)
                     /locus_tag="ACGE36_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFF8385252.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DAXWLH010000373          685 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.384, whole genome shotgun sequence.
ACCESSION   DAXWLH010000373 DAXWLH010000000
VERSION     DAXWLH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.384"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            87..>685
                     /locus_tag="ACGE36_002962"
     CDS             87..>685
                     /locus_tag="ACGE36_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFF8385253.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILP"
BASE COUNT          278 a           90 c          119 g          198 t
ORIGIN      
        1 aacaaattaa aaaaaattat atcttcttaa acaatgataa tattaaaatg caagcgcttg
       61 taaaaaataa ggagaagagg taaaaaatga agaaaaaact attaacttta tttcttgtga
      121 tgtctttctc aatcgttttg agtgtgtact acaatactat tgttttttca gaacaaataa
      181 actacacaga agaacagtat aatatagaaa aagaaaaagc tttaaaagtt ggttatagtg
      241 aagaacagtt taagcagatt atggaaatac ctacaaattt atcaaatgaa aatagtgaaa
      301 caagaatagt aaattatact atgacttcta atcaaaccaa agtcattaat aaagcaatgg
      361 agcaaattgg aaagccatac gaatggggag catcgggacc aaattctttt gactgtggtg
      421 gtttagtgaa atatgtttac aaacaagctg taaatattga actgcctatg ggaaccacca
      481 atcaagagca atacggtaca gaggtaagtt taaattcact taagcctggt gatttattat
      541 tttacggcaa tcgaggtgca acttatcatg taggtatata taaaggtaac ggtgtaatga
      601 ttcatgcacc gcaacctgga gaaactgtaa aagaggttaa catacaatat ttttatccta
      661 actttgcaaa aagaattttg cctga
//
LOCUS       DAXWLH010000374          674 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.385, whole genome shotgun sequence.
ACCESSION   DAXWLH010000374 DAXWLH010000000
VERSION     DAXWLH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.385"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..104
                     /locus_tag="ACGE36_002963"
     CDS             <1..104
                     /locus_tag="ACGE36_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8385254.1"
                     /translation="LESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            160..657
                     /locus_tag="ACGE36_002964"
     CDS             160..657
                     /locus_tag="ACGE36_002964"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8385255.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          241 a           96 c          131 g          206 t
ORIGIN      
        1 ggctagagag tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac
       61 aacgacgttt tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac
      121 gggtgattta tagtatacta tgtcaaaagg aggagttaga tgaacaatca aatcacacta
      181 gcgtatttag attatgatga tctagatgct ttacaagttg taagtgttgt cagtttttat
      241 gaatcgttta tcgatggcgc agatccactt gatatgcaag gatatttaca gacacaattg
      301 acaactgaaa tattggcagt agaattggca cagtcaacca gtaaatttat tggtattaag
      361 gatcatcaaa tactcattgg ttatatgaaa gtcaatgatg aaaaagatgc tatagagatt
      421 caacggctct atctgctcaa agattatcaa aacaaagggt tagggcaacg gttactcgat
      481 gaagcgaatc gttatgctaa aacgaaacaa aaacgttatt tacgtttgac tgtttatgaa
      541 aaaaatcatg caggtattcg tttttatgaa cgtcatggat ttaaaaaaat cgggatcaaa
      601 cattttcctt taggaaaaca agatcgtatt tgtccgattt tagaaaaaga aatctaaaaa
      661 aacgtcctca cttt
//
LOCUS       DAXWLH010000375          669 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.386, whole genome shotgun sequence.
ACCESSION   DAXWLH010000375 DAXWLH010000000
VERSION     DAXWLH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.386"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>669
                     /locus_tag="ACGE36_002965"
     CDS             <1..>669
                     /locus_tag="ACGE36_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8385256.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SLERF"
BASE COUNT          202 a          141 c          149 g          177 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgcagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgattttc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccacaga aaagagcagt ttcaaaatga agaatcacta
      661 gagcgtttc
//
LOCUS       DAXWLH010000376          652 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.387, whole genome shotgun sequence.
ACCESSION   DAXWLH010000376 DAXWLH010000000
VERSION     DAXWLH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.387"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(67..228)
                     /locus_tag="ACGE36_002966"
     CDS             complement(67..228)
                     /locus_tag="ACGE36_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFF8385257.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            298..652
                     /locus_tag="ACGE36_002967"
                     /pseudo
     CDS             298..652
                     /locus_tag="ACGE36_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          230 a          136 c           96 g          190 t
ORIGIN      
        1 agttctattt ttctttcttg taatatatgc tatagcattt tttatcactt tcctaactac
       61 tcctctctat tttcttactc cataactaat tgcatccact ggacaagcat ttttacatcg
      121 tccacatctg atacactcca gatgattgca attctcaact gaatctttat tcatttggca
      181 aactttttta cattttccac aattaataca cttatcctta tcctgaatcg gctacactag
      241 actagacaga aaaaataagg tgtgtagaat aataaaaaac acactggagg atttttcatg
      301 ccaagacgtc aacgaagaac ctattcaaaa gaattcaaac aacaaatcgt cgatctctat
      361 ctcgctggta agcctcgcgc agaaattatt cgagagtatg agcttacgcc ttcttctttc
      421 gataaatgga tgaagcaagc acaatcaacg ggctcattca aagaaagaga caacttaaca
      481 ccagaacaag cagaattgat cgcactaaga aagaaaaata agcaactcga aatggagaat
      541 gatattttaa agcaagcggc gctgatattc ggacgaaaag acaagtaatt gatgccaaca
      601 agcataaata ttccatatca gcgatgtgca aaattctaaa tatttctcgt ca
//
LOCUS       DAXWLH010000377          648 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.388, whole genome shotgun sequence.
ACCESSION   DAXWLH010000377 DAXWLH010000000
VERSION     DAXWLH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.388"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
BASE COUNT          226 a          132 c           81 g          209 t
ORIGIN      
        1 ctttttatag aatagaacta gtgttgtttt catagactct cctttcttca tatttccaat
       61 taatcacaaa ttcacggcat tactagaaaa attaaagggg cgcagaagtg gaaaaatact
      121 cttcgacttt ccctacaaac caggccatct tgtgtttaat aactatacca aatctaaggt
      181 acctctaaat ctaaattcca tttgtggcgg tgacctccaa tttttaactc ttctatctcc
      241 catccagtat acataacctt gatacttcga ccacttatat atcgaacaca caaataccta
      301 tccattgcag gagtactacc taacttagtt gctacttgca tctaaataac agctgaaaag
      361 ataggactta ctcttagtaa agtatctgaa ctattaattt ttaaactatc atttttatcc
      421 tcaactacat gcacatctgt tgcaacttca ctttctgtct catccagata aatgattaag
      481 gaatcgggaa ttgcaatatc ttcagctgac gctggaatag ttgagctaaa ttagtcatca
      541 tatctttatt ataaaataac caacagcaaa aaatacaatg aaaaacaaac ttggaactaa
      601 tgtatcatta ataaatgttg aaaaattcag caactcatta ctgtatga
//
LOCUS       DAXWLH010000378          638 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.389, whole genome shotgun sequence.
ACCESSION   DAXWLH010000378 DAXWLH010000000
VERSION     DAXWLH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.389"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            8..607
                     /locus_tag="ACGE36_002968"
     CDS             8..607
                     /locus_tag="ACGE36_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8385258.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          243 a           94 c          143 g          158 t
ORIGIN      
        1 tctacaaatg aacggttttg aaaagagagc acaagagaaa aagaaagaga ttttacaagc
       61 agcttttgac ttgatgaaca cagacatagg aaccgctgga gtgacaatgg ataaggtagc
      121 tgagaacgct caggtaagta aagcgacgat ttttaaatat tttggcagca aggaagggct
      181 gatttatgaa gtattttttg catttatgaa tgatttaggg caagaagcga aagcgttgat
      241 tgcggaaaat cttccatttg aagaaacact gattgccatg agtgaaaaca aaatccgtta
      301 tctcaatcga gtgaacaaac aattttatat cgatttgatg acatactata caaaaaaaga
      361 aaaaaaagaa ttttctgacg cgatggatgc gtatacgcaa gaaagtttca gtatcatgct
      421 tgatcttttc catcgagggc ggaaagaagg aaaagtggat ttgaaatatt cggatgagtt
      481 cttgatcctc tattttcaag caatggtaga aggaatttct aagccggaga tatacgcaaa
      541 actgcttcca tatacagcag aatggacaga agtcctgatc aagggaatcg caccgaagaa
      601 gaaataaagc ataaaaaagc cgaaactgac gataaaat
//
LOCUS       DAXWLH010000379          621 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.390, whole genome shotgun sequence.
ACCESSION   DAXWLH010000379 DAXWLH010000000
VERSION     DAXWLH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.390"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            129..>621
                     /locus_tag="ACGE36_002969"
     CDS             129..>621
                     /locus_tag="ACGE36_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFF8385259.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWMPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDVDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLE"
BASE COUNT          232 a          101 c          117 g          171 t
ORIGIN      
        1 gtattttttt aacttattgt ttaagagtga tacgcgaatt gtaaaattaa gttagaaaaa
       61 taaaaaggca tttatggtac actcaaattg tatttccgac gaaagaaaac aaaggagtgt
      121 aaccataaat gacctacaaa catcttacca tagacgaact gacaatgata gaatcatatt
      181 atcttcaaca taataagccg gttgaaatcg ctaaccgaat gggacgtgct atacaaacta
      241 tttataatgt agtcaataag ttcaagcaag gcaagactgc tcttgattat tggcaccagt
      301 ataaagaaaa taagaaaaaa tgtggtagaa aagtcattca attacctgct catgaagtag
      361 attacattaa agagaaagtc actcttggtt ggatgcctga cgtcattatc gggcgaaaag
      421 aaaggcctgt ttcatgcggt atgagaacac tttatcgttt attttctaaa ggaatatttg
      481 atgttgacac actaccgatg aaaggtaaaa gaaaacccaa tggccatcag gaaaaacggg
      541 gaaaacaaca gtatcagcgc tcaatccatg atcgacctga taattatcct gatttcaatt
      601 ctgagtttgg tcaccttgaa g
//
LOCUS       DAXWLH010000380          579 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.392, whole genome shotgun sequence.
ACCESSION   DAXWLH010000380 DAXWLH010000000
VERSION     DAXWLH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.392"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..>579)
                     /locus_tag="ACGE36_002970"
     CDS             complement(<1..>579)
                     /locus_tag="ACGE36_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8385260.1"
                     /translation="HIRASLKDAVEEGLIPFDPTRKAVIKGKDSLKPKEDKYLDYDQF
                     KSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDIDFEDQIITINKTWNYK
                     LNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLV
                     SSNAVSKYLRKKLKELGIEKQFTLHGLRHTHA"
BASE COUNT          167 a           95 c           85 g          232 t
ORIGIN      
        1 atgcatgtgt atgccttagt ccatgcaaag taaattgctt ttcaatccct aattctttta
       61 attttttgcg caaatatttg ctgacggcgt ttgatgaaac taatccattt tttaagttga
      121 aaaaaacaaa attatgaggg tttttgattt caaaattctc gaataattct ttttgattga
      181 ttttaaactt ttttaagaga tcaatcgtat gtttatcaat ggaaattttc ctatttgaag
      241 tttcgttttt tgtttttccc cattcattta atttataatt ccatgtttta ttaatagtga
      301 tgatttgatc ttcgaaatcg atatcttccc atgttaatcc tagaagttca gcaaatcgca
      361 ttccagtggc accagcaact aacacgagca taggagaaga gtactgcgca gaaaggtttt
      421 cttctacgag tttcattaaa gatttaaatt gatcataatc taaatattta tcttcttttg
      481 gcttcaatga atcttttcct ttgattactg cttttctagt tggatcaaac ggaattaatc
      541 cttcttctac ggcatctttc aacgacgctc taatatggt
//
LOCUS       DAXWLH010000381          575 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.393, whole genome shotgun sequence.
ACCESSION   DAXWLH010000381 DAXWLH010000000
VERSION     DAXWLH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.393"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            111..518
                     /locus_tag="ACGE36_002971"
     CDS             111..518
                     /locus_tag="ACGE36_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385261.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          220 a           74 c          105 g          176 t
ORIGIN      
        1 aattagctaa tatttccgtt gacaaagctt tgttactatt gttaaactaa attgactagc
       61 aaaagtgaaa cataaattta ttaaataaat gtaaaaatta ggagaaatca atgtcttata
      121 gagtacaatt aataatttca gaagatgtag aaagtcagca atttggcact aatgttatta
      181 ataaagtaat taatccagct ctttctatta atgcgccttt aattcctact gctttatcat
      241 tttctgtaac agctgtggtg tctgagatag aggatacaga aaagataaaa atagtagaaa
      301 ttgaagtttt aaacaagaat gaaaaacaaa ttttttctac gggtgaagta tcggtaagct
      361 tgcctcctca agttaacgat ataaacttta atataaacgc cagaaatgtc ttggttgaag
      421 aagcaggaga gcattatgct gttgttaaat ttaatggaac tgagattgga aggcaaatct
      481 ttgatatcaa ggtcaacaaa ccagtggaaa aaaattaagg agacgatacg gatgcttgat
      541 atagtaccat caaatacaca tcggagtggt agctt
//
LOCUS       DAXWLH010000382          573 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.394, whole genome shotgun sequence.
ACCESSION   DAXWLH010000382 DAXWLH010000000
VERSION     DAXWLH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.394"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            68..>573
                     /locus_tag="ACGE36_002972"
     CDS             68..>573
                     /locus_tag="ACGE36_002972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385262.1"
                     /translation="MFNQKKEKQFKYAIKKKKGGGGAASFVIGAVFLGIGLISYPTLS
                     FASETTEINQSSEIQSSLTNSVLKESNSEEKQNIDMSEDSSINNDLELDSKATVSGSE
                     NSFDETTNNLETTNLSEDSIEVQSESIIQNEDSTDNDAVQELEMNPHEDNGDDISVEN
                     QMTEATEE"
BASE COUNT          227 a           73 c          104 g          169 t
ORIGIN      
        1 ttcataaaat gaacgattat ttcgtctctt tttgattttt atactgtaat aaataaggag
       61 gaaaattatg tttaatcaaa agaaagagaa gcagttcaaa tatgcaatca aaaagaaaaa
      121 aggtggcggg ggagcagctt cctttgtcat aggtgcagta tttcttggaa tagggttaat
      181 ttcttatcct acattatctt ttgctagtga aacaacagag ataaatcaat caagtgaaat
      241 ccaatctagt ttgactaatt ctgttctaaa agaaagtaat tcagaagaaa aacaaaatat
      301 tgatatgtcg gaagatagtt ctataaataa cgatctagaa ctagattcga aagcaaccgt
      361 ttctggatca gaaaattcat ttgatgaaac tactaacaat ctagaaacta caaatttatc
      421 tgaagatagt attgaagttc agtcagagag tattattcaa aatgaagact cgactgataa
      481 tgatgctgtt caagaattag aaatgaatcc tcatgaagat aacggtgatg atatttcagt
      541 tgagaatcag atgacagaag ctacagaaga aaa
//
LOCUS       DAXWLH010000383          568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.395, whole genome shotgun sequence.
ACCESSION   DAXWLH010000383 DAXWLH010000000
VERSION     DAXWLH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.395"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>568
                     /locus_tag="ACGE36_002973"
     CDS             <1..>568
                     /locus_tag="ACGE36_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFF8385263.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DAXWLH010000384          544 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.396, whole genome shotgun sequence.
ACCESSION   DAXWLH010000384 DAXWLH010000000
VERSION     DAXWLH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.396"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            1..544
                     /locus_tag="ACGE36_002974"
                     /pseudo
     CDS             1..544
                     /locus_tag="ACGE36_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT          187 a           98 c           97 g          162 t
ORIGIN      
        1 cacaattgtg taccagataa gattaaaaac cgaagaaata ctgatcaact gaagtaacgc
       61 gatacagtga ttattgcttg tgttatatgg ggcataatta atggctatac tagccaaaga
      121 gccacgtata gagcggtttg ttccgtctta tttcctaatg gtgactttcc tagtaggagt
      181 cgattcactc gcttaagttc aaacttagct tacactatca agattattcg atactttttc
      241 atcaagaagc tcaccaaagg tgaactagtc ggaattatag atagttttcc tagtccatta
      301 tgtaaaccag ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat
      361 aactcaacaa aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact
      421 ggctttccga ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag
      481 actttatcgg aagaagcgaa tcttcccaat atcctagggg acaaaggata tattagccat
      541 aaaa
//
LOCUS       DAXWLH010000385          541 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.397, whole genome shotgun sequence.
ACCESSION   DAXWLH010000385 DAXWLH010000000
VERSION     DAXWLH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.397"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            278..490
                     /locus_tag="ACGE36_002975"
     CDS             278..490
                     /locus_tag="ACGE36_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385264.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
BASE COUNT          209 a           84 c           73 g          175 t
ORIGIN      
        1 tagcttccga tttcttatta cttgaacatc cacttaaaat aataaaaaat attgcaaaca
       61 tagagacaaa ccatagttgc tttttcttca ttttattcct tctttcgttg tttattttta
      121 gtatatcaaa taacgaaagt agcaacaaac ctcatttgga tacacggaga acgaatactc
      181 ataaaaatcc acctttctat agaaaatttt tctcttgata ttgtgattca ttacaaaaaa
      241 gagctataat aaagaaaaaa caagaaggag cattgaaatg tacgctgata aaaagaaaat
      301 cgaatggttg ctgtttgata gtggtaaatc agttcgttct atatccatag aatctggcgt
      361 acccttaact acagtaagtg accttgccaa caaacgctct agtatagaca acatgagatt
      421 cataaatgct atgaaattaa ataaattggc tgaagaaatc gttgatatta atacttcaac
      481 taacaaataa aataagaaaa gccaaaatta tataaggctt ttcttatttt ttattgacta
      541 t
//
LOCUS       DAXWLH010000386          532 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.399, whole genome shotgun sequence.
ACCESSION   DAXWLH010000386 DAXWLH010000000
VERSION     DAXWLH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.399"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..238
                     /locus_tag="ACGE36_002976"
     CDS             <1..238
                     /locus_tag="ACGE36_002976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385265.1"
                     /translation="KKKFKQQFKKILTAEIDVAISQSSTKYDPAFQKLIDKCLFIIKI
                     KEKFMSAFFIKVVMIIRLLIVFFRKYGIIVLTKR"
BASE COUNT          194 a           87 c           80 g          171 t
ORIGIN      
        1 aaagaaaaaa ttcaagcaac aattcaaaaa aatcttaact gctgagattg atgtagcaat
       61 atctcaatca tcaacaaagt acgatcctgc ctttcaaaag cttattgata aatgtttatt
      121 cattataaaa ataaaagaaa aatttatgtc agcttttttc atcaaagtgg taatgataat
      181 tcgtctatta attgtatttt ttaggaaata tggtataatt gttttaacga aaaggtaatt
      241 catgatacac caaaaaccac tagctggcgg gctagtggtt ttcttttacg ttctattcag
      301 attaacgggt aggcttactg tcttttttgg caataatttt gcaatcaagc catttacaga
      361 tgtaatgcgc ggatacttct gctaagattg caaaaaacat atcgaaaagg taattcataa
      421 tacaacctcc tccccttgcc ataataggcc ggggtagcag acagcataaa tattatacca
      481 taactgaaaa tttcatttta gtacatttca atatattgat gaaccagcac aa
//
LOCUS       DAXWLH010000387          524 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.400, whole genome shotgun sequence.
ACCESSION   DAXWLH010000387 DAXWLH010000000
VERSION     DAXWLH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.400"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(152..346)
                     /locus_tag="ACGE36_002977"
     CDS             complement(152..346)
                     /locus_tag="ACGE36_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385266.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          163 a          110 c           79 g          172 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caat
//
LOCUS       DAXWLH010000388          515 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.401, whole genome shotgun sequence.
ACCESSION   DAXWLH010000388 DAXWLH010000000
VERSION     DAXWLH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.401"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
BASE COUNT          230 a           71 c           83 g          131 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaa
//
LOCUS       DAXWLH010000389          507 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.402, whole genome shotgun sequence.
ACCESSION   DAXWLH010000389 DAXWLH010000000
VERSION     DAXWLH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.402"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(138..452)
                     /locus_tag="ACGE36_002978"
     CDS             complement(138..452)
                     /locus_tag="ACGE36_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385267.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          205 a           82 c           58 g          162 t
ORIGIN      
        1 tctagagaaa ttaattcatc ctaacataaa ataattgttc actcaaaagt ttttctagaa
       61 caattaaaac agcaggcttc acattcgcta tacatttgta agaagtaata attcctgtac
      121 taaaaggcat aattgcttta attatttttt gacttctctt caaacacaag aagtagttgt
      181 gtaataattg cagaaataag gattccaata atagatataa aatttgacat ggaaacgtct
      241 ataacattaa aagaataaag tgttgaagaa agtaaactga ttgaaataac ccctccgtaa
      301 aaaatagtct gtgaatatac ccactttttt tcagaagacg tacttcgttt ggttctaaaa
      361 cctatccctg catttctact cttatcaaaa aaccaaatat ttagaagtaa taatattgct
      421 attattaaga taaaattact gtaaaaatcc aaaagctatc ctcctagcaa ctatttaaat
      481 acccaaaaaa attacctcta gataata
//
LOCUS       DAXWLH010000390          501 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.403, whole genome shotgun sequence.
ACCESSION   DAXWLH010000390 DAXWLH010000000
VERSION     DAXWLH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.403"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..491
                     /locus_tag="ACGE36_002979"
     CDS             <1..491
                     /locus_tag="ACGE36_002979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385268.1"
                     /translation="GFLVGLQKRFEEQISVLAERYEVMVLIPKAVEESFDKYSKDFSS
                     YSTNTPPVSVDHAYQEGVREAKKIDLTKSTVDTDYSSLVGRIIRFNQGVTRGLIAEIT
                     SVSDDLLNLLVMNCVSEYASEDNPSFYHWKVDKEYDYEILSFNDPMSKKFKKNKVDIM
                     EN"
BASE COUNT          181 a           64 c           99 g          157 t
ORIGIN      
        1 ccggattttt agtaggtctg caaaaacgat tcgaggaaca aatttctgtt ttggccgaac
       61 ggtatgaagt aatggttttg attccaaaag ctgtagaaga atcctttgat aaatatagta
      121 aagatttttc tagttatagt actaatactc caccggtatc tgtggatcat gcgtaccaag
      181 agggagtaag agaagctaaa aaaatagatt taacaaaaag cactgttgat acagattata
      241 gtagtttagt aggtcgtatt attaggttta atcaaggagt aactcgtgga ttaatagctg
      301 aaatcactag tgtttctgac gatctattaa atttgttggt aatgaattgt gtttcagaat
      361 atgcttctga agataaccct tctttctatc attggaaagt agataaggag tacgactatg
      421 aaatcctttc tttcaacgat cccatgagta aaaagtttaa aaaaaataaa gtagacataa
      481 tggaaaacta aaaaaagtga t
//
LOCUS       DAXWLH010000391          465 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.404, whole genome shotgun sequence.
ACCESSION   DAXWLH010000391 DAXWLH010000000
VERSION     DAXWLH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.404"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            78..>465
                     /locus_tag="ACGE36_002980"
     CDS             78..>465
                     /locus_tag="ACGE36_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8385269.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHY"
BASE COUNT          169 a           67 c           77 g          152 t
ORIGIN      
        1 tttagggtag aaaaaaagaa agtccattct gtaaaattaa agttaccaca accgaaattt
       61 tataggagga ctttctcatg cccaatttta ctacagaaat tatggaaaca ctaatcaata
      121 aaggtgattt agatgaatta tttcgatgtc atttagaatt agctgttaat tcattattgc
      181 aggctgaatt aacagctttt cttgattatg aaaaatatga tcgtgctggg ttcaattcag
      241 gtaattcccg taatgggaat tattcacgtt cattcagaac agagtatgga gaattaaatt
      301 tggtaattcc tagagataga aatggtaagt tttctcaaca aactttacct gcttataaaa
      361 gaaccaatga ctctttagaa accactatta ttcagctatt tcaaaaagga attactatgt
      421 ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca ctata
//
LOCUS       DAXWLH010000392          464 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.405, whole genome shotgun sequence.
ACCESSION   DAXWLH010000392 DAXWLH010000000
VERSION     DAXWLH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.405"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>464
                     /locus_tag="ACGE36_002981"
     rRNA            <1..>464
                     /locus_tag="ACGE36_002981"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          141 a          103 c          131 g           89 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagt
//
LOCUS       DAXWLH010000393          445 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.406, whole genome shotgun sequence.
ACCESSION   DAXWLH010000393 DAXWLH010000000
VERSION     DAXWLH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.406"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            99..293
                     /locus_tag="ACGE36_002982"
     CDS             99..293
                     /locus_tag="ACGE36_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385270.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          189 a           56 c           76 g          124 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attgg
//
LOCUS       DAXWLH010000394          441 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.407, whole genome shotgun sequence.
ACCESSION   DAXWLH010000394 DAXWLH010000000
VERSION     DAXWLH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.407"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..338
                     /locus_tag="ACGE36_002983"
     CDS             <1..338
                     /locus_tag="ACGE36_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8385271.1"
                     /translation="KEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRR
                     TIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVT
                     DTLVSMFTE"
BASE COUNT          167 a           77 c           64 g          133 t
ORIGIN      
        1 caaaagaaga agcgaatacc ttcttatccg gcatgattga gaaatggaag aaaaactatc
       61 ctaaagtgac gcagtcactc atagaaaacc aagacttatt aactttttat gattttccac
      121 ctagcattcg tagaaccatt tactcaacca atctaatcga gtctttcaat aagcaaatta
      181 aaagatacag ccgtagaaaa gagcagtttc aaaatgaaga atcactagaa cgctttctag
      241 tcagcatttt tgatacatac aatcaaaaat ttctaaacag aagccataaa ggttttcaac
      301 aggtaaccga tacattagtt tcaatgttta ctgagtaact aattattttg caggaggaca
      361 atttatttac acaaaattat tgacgctccc aaactgatca ttaagtttgg cttttatgtc
      421 ataattgaat tttataaagt t
//
LOCUS       DAXWLH010000395          434 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.408, whole genome shotgun sequence.
ACCESSION   DAXWLH010000395 DAXWLH010000000
VERSION     DAXWLH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.408"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            6..>434
                     /locus_tag="ACGE36_002984"
     CDS             6..>434
                     /locus_tag="ACGE36_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFF8385272.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASID"
BASE COUNT          153 a           77 c           86 g          118 t
ORIGIN      
        1 aatttatgaa aaaagcaacg ctcgtaaaat taggtcttag ttttgcagga attctagcac
       61 ttgccgcatg cggcagcagt tcaagtaaca gttcttcaga gacagaagat ggaaaagcta
      121 aaatcacttg gttaaatatt ttgcatcatg cttcaccgcc tacagacacg attgtgaact
      181 tgatcgaaga aaaaaccgat acggaactga cattcaactg gattcctgat gcttcaaaag
      241 aagaaagatt aacaactgca ttggcttctg atgaactagg agatatcgtt acacttcaaa
      301 ttgcgatgtt gaaaaactct tctgtccgca atgcgttaaa atcaggcgta ttctgggatg
      361 tatcagaata tttaaaagac tataaaaatt taagtcaaat ttctgaagat agaatcgaag
      421 ctgcgagtat cgac
//
LOCUS       DAXWLH010000396          422 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.409, whole genome shotgun sequence.
ACCESSION   DAXWLH010000396 DAXWLH010000000
VERSION     DAXWLH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.409"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
BASE COUNT          133 a           61 c           65 g          163 t
ORIGIN      
        1 tattttaaat ttttacatac ctgtttatga aaaactaaat aatctgtcaa aaaaatatga
       61 ttgttcattt ctaaatatgt tttccaattt taaaatcttt tttagcattt ttgtttcctt
      121 ttcatatggt ttttcgttgt taatagtgac taggatgata ttcctatttt gaccattgac
      181 aattaaatat aatttttgaa taacgttccc tgctattcga gctaagaatt caagtacgcc
      241 atgacctatt tagcgataac tacttagaga tttctttttg ttttgggaag acaaaatgcc
      301 atgacaatag cagtagataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      361 aaattcctat gtgcttaccc aagtcaattc aaaaatttta gttcatttat aaggggatga
      421 tt
//
LOCUS       DAXWLH010000397          421 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.410, whole genome shotgun sequence.
ACCESSION   DAXWLH010000397 DAXWLH010000000
VERSION     DAXWLH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.410"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..323
                     /locus_tag="ACGE36_002985"
     CDS             <1..323
                     /locus_tag="ACGE36_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8385273.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          156 a           76 c           59 g          130 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
      421 t
//
LOCUS       DAXWLH010000398          415 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.411, whole genome shotgun sequence.
ACCESSION   DAXWLH010000398 DAXWLH010000000
VERSION     DAXWLH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.411"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            complement(<1..339)
                     /locus_tag="ACGE36_002986"
     CDS             complement(<1..339)
                     /locus_tag="ACGE36_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8385274.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAXWLH010000399          406 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.412, whole genome shotgun sequence.
ACCESSION   DAXWLH010000399 DAXWLH010000000
VERSION     DAXWLH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.412"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            126..406
                     /locus_tag="ACGE36_002987"
                     /pseudo
     CDS             126..406
                     /locus_tag="ACGE36_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          158 a           49 c           71 g          128 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga aatgctaatg
      181 cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac caaaaatctg
      241 gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa aaagaagatt
      301 gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca atgaaatatt
      361 taattgagca ttactttgat aatttcaaag tagatagtat tgaaat
//
LOCUS       DAXWLH010000400          391 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.413, whole genome shotgun sequence.
ACCESSION   DAXWLH010000400 DAXWLH010000000
VERSION     DAXWLH010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.413"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            1..375
                     /locus_tag="ACGE36_002988"
                     /pseudo
     CDS             1..375
                     /locus_tag="ACGE36_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
BASE COUNT          145 a           64 c           65 g          117 t
ORIGIN      
        1 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
       61 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
      121 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
      181 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
      241 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaaagattcg
      301 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
      361 attctcttca attaaatact ccttatcaat a
//
LOCUS       DAXWLH010000401          386 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.denovo.414, whole genome shotgun sequence.
ACCESSION   DAXWLH010000401 DAXWLH010000000
VERSION     DAXWLH010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.denovo.414"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..>386
                     /locus_tag="ACGE36_002989"
     CDS             <1..>386
                     /locus_tag="ACGE36_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8385275.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRAS"
BASE COUNT          154 a           51 c           75 g          106 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcat
//
LOCUS       DAXWLH010000402        10859 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.086, whole genome shotgun sequence.
ACCESSION   DAXWLH010000402 DAXWLH010000000
VERSION     DAXWLH010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.086"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            213..437
                     /locus_tag="ACGE36_002990"
     CDS             213..437
                     /locus_tag="ACGE36_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385276.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            554..1051
                     /locus_tag="ACGE36_002991"
     CDS             554..1051
                     /locus_tag="ACGE36_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFF8385277.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            1138..1530
                     /locus_tag="ACGE36_002992"
     CDS             1138..1530
                     /locus_tag="ACGE36_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8385278.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1514..3961
                     /locus_tag="ACGE36_002993"
     CDS             1514..3961
                     /locus_tag="ACGE36_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8385279.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3964..6147
                     /locus_tag="ACGE36_002994"
     CDS             3964..6147
                     /locus_tag="ACGE36_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8385280.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            6144..7145
                     /locus_tag="ACGE36_002995"
     CDS             6144..7145
                     /locus_tag="ACGE36_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8385281.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            7160..8074
                     /locus_tag="ACGE36_002996"
     CDS             7160..8074
                     /locus_tag="ACGE36_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8385282.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8388..8435
                     /locus_tag="ACGE36_002997"
                     /pseudo
     CDS             8388..8435
                     /locus_tag="ACGE36_002997"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8451..10370
                     /gene="tet(M)"
                     /locus_tag="ACGE36_002998"
     CDS             8451..10370
                     /gene="tet(M)"
                     /locus_tag="ACGE36_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8385283.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10468..>10859
                     /locus_tag="ACGE36_002999"
     CDS             10468..>10859
                     /locus_tag="ACGE36_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8385284.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARY"
BASE COUNT         3451 a         1974 c         2286 g         3148 t
ORIGIN      
        1 agaaacaata caaaaatata tagctctatt gaagcgaaat aaagtccagc tcataagact
       61 aaataaatat agagtattag ctggcaatca agaattaaac ttgatatagg tctactacat
      121 aaatactttt caatataaac acgatggaac gcggagtgcg gtgaaagtcg cacgctccgt
      181 gtgaatgggg ggaaaagttt gagatattta taatatcagg aacttaccta tccatatata
      241 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
      301 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      361 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      421 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      481 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      541 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      601 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      661 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      721 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      781 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      841 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      901 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      961 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
     1021 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
     1081 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
     1141 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
     1201 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1261 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1321 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1381 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1441 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1501 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1561 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1621 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1681 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1741 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1801 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1861 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1921 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1981 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     2041 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     2101 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     2161 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     2221 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2281 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2341 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2401 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2461 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2521 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2581 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2641 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2701 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2761 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2821 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2881 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2941 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     3001 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     3061 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     3121 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     3181 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3241 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3301 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3361 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3421 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3481 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3541 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3601 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3661 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3721 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3781 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3841 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3901 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3961 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     4021 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     4081 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     4141 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     4201 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4261 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4321 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4381 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4441 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4501 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4561 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4621 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4681 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4741 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4801 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4861 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4921 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4981 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     5041 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     5101 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     5161 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     5221 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5281 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5341 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5401 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5461 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5521 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5581 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5641 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5701 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5761 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5821 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5881 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5941 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     6001 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     6061 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     6121 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     6181 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6241 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6301 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6361 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6421 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6481 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6541 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6601 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6661 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6721 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6781 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6841 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6901 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6961 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     7021 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     7081 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     7141 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     7201 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7261 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7321 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7381 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7441 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7501 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7561 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7621 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7681 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7741 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7801 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7861 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7921 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7981 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     8041 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     8101 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     8161 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     8221 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8281 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8341 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8401 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8461 ttaatattgg agttttagct catgttgatg cgggaaaaac taccttaaca gaaagcttat
     8521 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
     8581 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
     8641 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
     8701 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
     8761 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
     8821 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
     8881 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
     8941 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
     9001 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
     9061 aaagcataag atttcagaat tgctccttgt accctgttta tcatggaagc gcaaaaagca
     9121 acatagggat tgagcagctt atagaagtga taacgaataa attttattca tcaacataca
     9181 gaaagaagtc tgaactttgc ggaaatgtct tcaaaattga atattcggaa gaaagacaac
     9241 gtcttgcata tgtacgcctt tatggcggaa tcctgcattt gcgggattcg gttagaatat
     9301 cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt gaattatgta
     9361 aaattgataa ggcttattcc ggggaaattg ttattttgca aaatgagttt ttgaagctaa
     9421 atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa aatccgctcc
     9481 ctctgctgca aacaactgtt gaaccgagca aacctcaaca aagggaaatg ttacttgatg
     9541 cacttttaga aatctccgac agtgacccgc ttctacaata ttatgtggat tctacgacac
     9601 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
     9661 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
     9721 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
     9781 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
     9841 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
     9901 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtatg
     9961 gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct cctattgtat
    10021 tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt agttttaaaa
    10081 tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa tattgtgcga
    10141 acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa atccctgctc
    10201 ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt agtgtttgtt
    10261 taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag ccccgtcgtc
    10321 caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag tgtattttat
    10381 gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa taaagatttg
    10441 aattaaagtg taaaggagga attaattatg aagtgtaatg aatgtaacag ggttaaatac
    10501 aaccaaattt taatttggct ttgcgtttta tcttttttta gcgtattaaa tgaaatggtt
    10561 ttgaacgtct cattacctga tattgcaaat gattttaata aaccacctgc gagtacaaac
    10621 tgggtgaaca cagcctttat gttaaccttt tccattggaa cagctgtata tggaaagcta
    10681 tctgatcaat taggcatcaa aaggttactc ctatttggaa ttataataaa ttgtttcggg
    10741 tcggtaattg ggtttgttgg ccattctttc ttttccttac ttattatggc tcgttttatt
    10801 caaggggctg gtgcagctgc atttccagca ctcgtaatgg ttgtagttgc gcgctatat
//
LOCUS       DAXWLH010000403         7593 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.127, whole genome shotgun sequence.
ACCESSION   DAXWLH010000403 DAXWLH010000000
VERSION     DAXWLH010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.127"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            189..1085
                     /locus_tag="ACGE36_003000"
     CDS             189..1085
                     /locus_tag="ACGE36_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFF8385285.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1235..2176)
                     /locus_tag="ACGE36_003001"
     CDS             complement(1235..2176)
                     /locus_tag="ACGE36_003001"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8385286.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2302..3423
                     /locus_tag="ACGE36_003002"
     CDS             2302..3423
                     /locus_tag="ACGE36_003002"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8385287.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3681..5183
                     /gene="eat(A)"
                     /locus_tag="ACGE36_003003"
     CDS             3681..5183
                     /gene="eat(A)"
                     /locus_tag="ACGE36_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8385288.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5250..5777
                     /locus_tag="ACGE36_003004"
     CDS             5250..5777
                     /locus_tag="ACGE36_003004"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8385289.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5988..7049
                     /locus_tag="ACGE36_003005"
     CDS             5988..7049
                     /locus_tag="ACGE36_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFF8385290.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7052..>7593
                     /locus_tag="ACGE36_003006"
     CDS             7052..>7593
                     /locus_tag="ACGE36_003006"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8385291.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHAD"
BASE COUNT         2532 a         1320 c         1616 g         2125 t
ORIGIN      
        1 caaattttca gacaattaca actattcaga aaacttttag gcgaatttta atagaagaat
       61 gatttatttc atcatatttt catacaaatg ttaaattttc atacaaatat aggaaacgtt
      121 ttactggctt tttactattt aaacaggcta tcataggaaa aagtgacaga gaaagtgagg
      181 cggaaaagat gaacaaaaat gacgtactgc tagttgcaaa aaataaatca ctttattttt
      241 tgccttccat ccgctcttta gatgtttggt ccggaaaaga tttactacag tcggaattca
      301 ttaaatcagc tagacaacag ttggcaaatt atcagctgat cgttttttta gattatggat
      361 ttgatatgga gatggctcga cgcgtgcgcc catttaccaa tgcaaaaatt gtgttatttt
      421 tttggaatca cttcaaagca gaacacgagg cgatgctgaa gcaggcacaa gatgaaccgg
      481 caatcgatga gatttatcat tttgattttt tggaagcaaa agaacttggg ctgaaacata
      541 atagttcatt ttattcgaaa catatgaatt tttcttctag cgaaccaaag acggatctat
      601 tttttggtgc aacgaataat gggagaaaag aacgagcaga aagctataag caggaattta
      661 taaaaagaga tgttacgacc aactatttca ttcttccaca tcgagggaat gaacaacctg
      721 gatatcttac ttatgaagag tacctgaaaa aaacagggga atcgcgcggg attctggaac
      781 tgttgcggga gggtcagcaa ggagtgactt tgcggacatt tgaatccatg tattttcaaa
      841 agaaactagt gacggataat caagcgattg ctcattatca tttttatcgt cctgaaaata
      901 tttttttgct tcgagaacgt gatttagatg aattaccgga atttctgcat acaccattta
      961 agccaatcct atcagagcta ttggaatttt atgatgcaga aaattgggca aaacgattta
     1021 tcactaacga ttccactatt tatgagcaat acgaatatga cctctcctct gttgaaaaca
     1081 aataaaaaac ctacattttg aagagattat gtactgaaac aaacaagcac aattcttatg
     1141 gcatcatgag agttgtgttt tctttttgat cttttattgg gcttaaaaaa agagaaccag
     1201 ctgctgattc agctgattct catcagagag ccttttattc tgcgccaagg attccttctt
     1261 ttgcatggac taattgggtc aaaaaggcac aatatggtgt agcaatatca tatttttgtc
     1321 cttttcgcca aatggcacca ttgatgtagt cgatttcagt caaacgatgg tttttgatca
     1381 ggtcttggta catagatggg taatgcaacc cgatgttgtc aggatcataa gtagaagcaa
     1441 tatgttggta cacttcttct tgatctagta tgattccttc ttttgctgcg atagaagcaa
     1501 attcagatac gattgttcga accatgctct ctgccgcttt ttgcgcgcct agttcagcaa
     1561 tattacaatc caaaatcgtg cataaaccat tcaatgttcc atttacacaa gcttttcgcc
     1621 aaatggaata acgaacgtta tggctataga tcggatttaa atttgcttct tgaaagactt
     1681 ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa gttctctagt tcgatttctc
     1741 catcacctaa caatgtgact tttccgggac cagctagacc agctgtccac atcgtgatcc
     1801 caaataagat atttttcttt ggcacatatt tttccagtac atcttcatga cctaatccat
     1861 ttagtaaaca caaaacataa gtattgtcag taataatgga ttggatcgaa caaaacatat
     1921 catctaattg attggctttt gttaatgcta cgatcaagtc cacgtgttcg ttgctttcga
     1981 tgatttcttc tggtgaatag attggcagtt ttgcgaccac ttctttaccg ttaaagtcag
     2041 caatcaaccc atttgtacga atggcttcaa tgtgagcagg ccaacgatcg atcaagagga
     2101 cctcatttcc gctttgatgt agcatcaatc cgattcggct gcccattgct ccggccccag
     2161 ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt tagtatatag
     2221 aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt gtcttactta
     2281 agaagaaagt gagggataca gatggatatt ttggcggcaa ttgattcatt taaaggaagt
     2341 gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg taaaggaaga
     2401 aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg
     2461 ctcaggggaa catggcgtac cattgatggg atggatctct tattccgtca gcgaaaaatc
     2521 agctatttat tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc tgcacaagca
     2581 ttggggttag atctacttga taaaccaacg gatcaaacta tccgacttgc ttccagttat
     2641 gccttaggag aacttattaa agatgcactg cttcaagaag tagaacaaat tattgtcact
     2701 ttaggaggaa gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct
     2761 ttagaagggt acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga
     2821 agaaacgttc ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat
     2881 acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg
     2941 ctagctttct tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag
     3001 gtggatttag atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt
     3061 ttgttgggag cgaagatggt ttctggtttc gatttagtca gccaaatcac tggattggaa
     3121 aaaaagatcg aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg
     3181 atccacggaa aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata
     3241 atcgctattt gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt
     3301 gtcttaagta ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca
     3361 ttagaaaaaa caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag
     3421 tagttgacaa aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg
     3481 atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg
     3541 agacaaggaa gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct
     3601 ttttgtgtgt atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt
     3661 tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg
     3721 acagccaagg cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac
     3781 taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca
     3841 aactacctta tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga
     3901 caaaagataa agaacgttta acctattacg tgttaaatga tattacggat tttgagatat
     3961 gggaaatcga aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat
     4021 tcagcacact atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg
     4081 acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac
     4141 aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg
     4201 gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt
     4261 atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg
     4321 ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag
     4381 ccgaatggtc tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata
     4441 ctgaatctag acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac
     4501 gatccaaagc aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa
     4561 aagatatcga atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac
     4621 ttttaagcgt agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt
     4681 ttacaatcga gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat
     4741 ccattatcca ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc
     4801 caaaacatct gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg
     4861 cggaatttgc agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc
     4921 ttgggatgga aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga
     4981 aaaaagtgga attggctaaa tctttatcac agccagctga actatataca tgggatgaac
     5041 cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga
     5101 aacctgccat gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga
     5161 ttatttctct tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg
     5221 ggaactttgc caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa
     5281 cttggaacgc acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac
     5341 agatgcaggc acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa
     5401 tcatccattc ccctcttcaa cgagcaagag atacagcaca agcagttgct gatcgtctca
     5461 gcttgccgct gaccgttgat gaacgattgg tagaaatgga ttttggggat tatgatggac
     5521 tgccaagcaa agatgagaac tttcaaaaag cacgtttagc gttcgctgta cgattcccaa
     5581 atggggagtc agtcttagat gtgtatgcac ggatcgttcc tttattgaaa gaatgcatcg
     5641 aagacgaaga aaatgtctat ctgttagttt gccataatgc cttgataaga gtgatcaatg
     5701 cttattttca tccgatgcct aatgaggggt tctttacatt catggtagat aatacagaat
     5761 tggtttctta tgagtaatta tgtttttgtg ttatttaaat aaaatatttc aaggcactgc
     5821 ttttgcttag cagtgccttt tttaggttat gacaattctt ttgataatat aaactcaacc
     5881 tttagaaatg aaatttaatt attttaataa aattattgaa attttgtttc tttagatata
     5941 tactctagtc atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt
     6001 caatatttat gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat
     6061 ccaatggctt tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat
     6121 atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg
     6181 tcgatatttc aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt
     6241 tgaaagcaat tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg
     6301 ccgactggtc acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata
     6361 tcgatgaatt gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt
     6421 atccacgtcc cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca
     6481 aaattcaagg tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac
     6541 tatatcaagg attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc
     6601 tagatctatt agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa
     6661 acgttcagga gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac
     6721 ctgtagatga agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg
     6781 caagagatgt catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa
     6841 gaaatacagc tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct
     6901 atatgggtga gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag
     6961 tagctaaagt tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact
     7021 atcaatttat aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac
     7081 aacggaacgc agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt
     7141 tcaactaatg aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag
     7201 tatcgaacca gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta
     7261 tatgggagcc ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac
     7321 attcggtgta gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac
     7381 tgtagctgtc gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct
     7441 gaatttgaca aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt
     7501 gatcggtgga ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa
     7561 taaacaagcc gaaatcagta aacacgcgga ttt
//
LOCUS       DAXWLH010000404         6461 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.139, whole genome shotgun sequence.
ACCESSION   DAXWLH010000404 DAXWLH010000000
VERSION     DAXWLH010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.139"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            207..467
                     /locus_tag="ACGE36_003007"
                     /pseudo
     CDS             207..467
                     /locus_tag="ACGE36_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            630..1295
                     /locus_tag="ACGE36_003008"
                     /pseudo
     CDS             630..1295
                     /locus_tag="ACGE36_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1331..1576
                     /locus_tag="ACGE36_003009"
     CDS             1331..1576
                     /locus_tag="ACGE36_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8385292.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1679..2548
                     /locus_tag="ACGE36_003010"
     CDS             1679..2548
                     /locus_tag="ACGE36_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8385293.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2529..3263
                     /locus_tag="ACGE36_003011"
     CDS             2529..3263
                     /locus_tag="ACGE36_003011"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8385294.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3296..4204
                     /locus_tag="ACGE36_003012"
     CDS             3296..4204
                     /locus_tag="ACGE36_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8385295.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4201..4743
                     /gene="sat4"
                     /locus_tag="ACGE36_003013"
     CDS             4201..4743
                     /gene="sat4"
                     /locus_tag="ACGE36_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8385296.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4836..5630
                     /locus_tag="ACGE36_003014"
                     /note="aph(3')-IIIa"
     CDS             4836..5630
                     /locus_tag="ACGE36_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8385297.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            5906..6448
                     /locus_tag="ACGE36_003015"
     CDS             5906..6448
                     /locus_tag="ACGE36_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8385298.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2235 a          962 c         1409 g         1855 t
ORIGIN      
        1 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttttaaat
       61 ttggaaagtt acacgttact aaagggaatg gagataaatt attagatata ctactgacag
      121 cttccaagaa gctaaagagg tccctagcgc ctacggggaa tttgtatcga taaggggtac
      181 aaattcccac taagcgctcg ggaccccttg taggaaaatg tcctaagtgt ggcaacaata
      241 ttgtattaaa aaaatcgttt tatggttgtt caaattatcc tgaatgtaag tttactttag
      301 ctgaacattt tagaaagaaa aaactaacca aaacgaatgt aaaagaatta ctggagggaa
      361 aagaaaccct ggtaaaagga atcaaaaaca aagagaaaaa gccctacaat gccgttgtaa
      421 aaattgggga aaagggatat attgatttta tctctttttc aaaataaaca tggggctgac
      481 ccaaaagtag ctcctatatg aaaaatagag gtctttccat ttagatagta gtggaaagac
      541 ctctatttat cttttgaaag tgaatacaaa aataaagcac actctgctat aatataggtg
      601 attaatccat ttgagagagg tgctttatca tgtatcaaaa ttataccata aaccaacttt
      661 gtttgcctat agatcttgaa attaaattag aagaaaatga ttttgctcat gccattgtgc
      721 agtttgttga ctctattccc gatgaagtct ttcttcctta ttatcaatct atgggtagac
      781 cccaatatca tcctcgtatg ctactttcca ttattctttg tgcctatatt caaggggttt
      841 attcgggccg ccaaatacaa aatatgctga tagatagtat tcggatgcgc tacttatctc
      901 aagagcaatt ccctaacttt cgtactatta atcgttttcg cgttcatccg attatgaacg
      961 atattctaga tcattcattt gttcaatttc gtgagctact ggttcaaagt ggcttaatca
     1021 gtggcagtgc tctatacatt gatggtacaa aaattgaagc ggatgcgaat aaatactctt
     1081 tcgtttggaa aaaaagtatt ttaaagtata aggatagcct tgatcaaaaa gcccttgaaa
     1141 actaccaaca aatggtggaa acaaaaatct tacctgagtt aatagatgat ttaaaggatg
     1201 aactttctat agaagagatt gaaaggattc gtcaatcttt ggaagaaaag gaaaatcaat
     1261 taattgaagc gattgaccaa accgaaacag tagaaggggg ctgacccatt attaataagg
     1321 agtggacaac atgattgatg taaatgagct attggaagaa gctattagag aaactgaaaa
     1381 tctaaatgat ggcgaagttt ttcttgttaa agatttattc aagggatatg tggggaatag
     1441 gatacccaga aaggatcgac ttctgcttgg aacgttattt ttaaaccatg ttagcaaaat
     1501 gaatggtaat ttaaaggcta ttgaaaagac ttcatctaat cagcagaggt ataaaaagac
     1561 aattgataag ttttaactat cttttgtgaa attaaaaaaa ttataaatat gttgagtaga
     1621 agcaaaagat ttggcttgga atttttacag cagtgaatcg ataaaaggag catgcttgat
     1681 ggtagataat attattaaat cagtagcaga gaaattatcc tctctgtctt atatagaagg
     1741 tattgtttta ggtggttcac gtgcaagggg cacccataca gaggattcgg atatagatat
     1801 cggcatctat tacaattcag aatcatttga cataaatact attaatcaat tcgctacaaa
     1861 gctggatgat gagcatagaa ataaccttgt tgtacctccc ggagcatggg gtgattggat
     1921 taatggcggc ggatggttag tcataaacgg gtatcatgtg gatttaattt tacgtgatat
     1981 taaacgtgtg gaacaaataa tgaaagatac agagcacgga attgttactg ccaattatca
     2041 gactgggcat ccccatggtt atattagtgc aatgtatcga ggagaattag cgattagcaa
     2101 aatactatat gctaagaatg aaagcttatg cgaattaaaa aaacaggcag aaacttatcc
     2161 caatgctttg cagaaaagtt tagttaactt ttttatgttt gaagcagggt tctctttaat
     2221 gtttgtaaaa gcaaattcgg gaacagacga taaatattat attgcgggtc atgtttttcg
     2281 tatagtttca tgtttaaatc aagtgttatt tgcatgtaat aatgcttatt gtatcaacga
     2341 aaagaaagct ataaaactgc ttgaaacttt tgaacataaa cctgaaaaat ataccgagaa
     2401 ggtaaatcat atttttgaag tactcggtat ctcacttttt gaatgctacg acatgaccga
     2461 gaagctttat aatgaagtga atgaaattgt atcggagata aataactttt taaacgagga
     2521 gagttcagat gaaagaaaac aaatatgatg ataatatatt ttttcaaaaa tacagtcaaa
     2581 tgagtcgctc gcagaaagga ctggctggtg cgggagaatg ggagactttg aaaaagatgc
     2641 tacctgattt taagggtaag cgtgtgcttg atttaggatg cggctatgga tggcactgta
     2701 tatatgcgat ggaaaacggt gcttcctctg tagtaggtgt tgatatttct cataaaatgc
     2761 tcgaagtagc aaaaggaaaa acccattttc cacagattga atatgaatgc tgtgccatag
     2821 aagatgtgga tttcccagag gagagctttg atgtaatact aagttcgctt gcgtttcatt
     2881 atgtagcaga ctatgagaat ttaataaaaa agatatatag gatgctgaag gctggtggca
     2941 atttagtttt tacagttgaa catcctgttt ttactgctca tggaacacaa gactggtatt
     3001 ataacgaaaa aggagaaata ctgcatttcc cggtggacaa ttattattat gagggcaaac
     3061 ggacagctat gtttttggaa gaaaaggtta caaaatatca tagaacactg accacatatc
     3121 taaatacact gctttcaaat agttttataa taaatcagat tgtggagcca cagccgccag
     3181 agaacatgat ggatattccg gggatggcgg atgaaatgcg acgcccaatg atgctgattg
     3241 tatcggcaaa aaagaagatg taataatata gaaaaaataa acgaggagta tgtaaatgag
     3301 atcagaaaaa gaaatgatgg atttagtact ttctttagca gaacaggatg aacgtattcg
     3361 aattgtgacc cttgaggggt cacgcgcaaa tattaatata cctaaagatg aatttcagga
     3421 ttatgatatt acatattttg taagtgatat agaaccgttt atatctaatg atgactggct
     3481 taatcaattt gggaatataa taatgatgca aaagccggag gatatggaat tattcccacc
     3541 tgaagaaaag ggattttcct atcttatgct atttgatgat tacaataaaa ttgatcttac
     3601 cttattgccc ttggaagagt tagataatta cctaaagggc gataaattaa taaaggttct
     3661 aattgataaa gattgtagaa ttaaaaggga catagttccg actgatatag attatcatgt
     3721 aagaaagcca agcgcaaggg agtatgatga ttgctgcaat gaattttgga atgtaacacc
     3781 ttatgttatt aaaggattgt gccgtaagga aattttattt gctattgatc attttaatca
     3841 gattgttcgc catgagctgc tgagaatgat atcatggaag gtcggcatcg aaacaggctt
     3901 taaattaagt gtaggcaaga actataagtt tattgaaagg tatatatccg aggatttgtg
     3961 ggagaaactt ttgtccacct accggatgga ttcctatgaa aacatatggg aagcattatt
     4021 tctatgccat caattgttca gggcggtatc cggtgaggtg gcggaaaggc ttcattatgc
     4081 ctatccggag tatgatagga atataacaaa atataccagg gacatgtata aaaaatacac
     4141 tggtaaaacc ggctgcctgg atagcacata tgccgctgat atagaagaga ggcgggaaca
     4201 gtgattacag aaatgaaagc agggcacctg aaagatatcg ataaacccag cgaaccattt
     4261 gaggtgatag gtaagattat accgaggtat gaaaacgaga attggacctt tacagaatta
     4321 ctctatgaag cgccatattt aaaaagctac caagacgaag aggatgaaga ggatgaggag
     4381 gcagattgcc ttgaatatat tgacaatact gataagataa tatatcttta ctaccaagac
     4441 gataaatgcg tcggaaaagt taaactgcga aaaaattgga accggtacgc ttatatagaa
     4501 gatatcgccg tatgtaagga tttcaggggg caaggcatag gcagcgcgct tatcaatata
     4561 tctatagaat gggcaaagca taaaaacttg catggactaa tgcttgaaac ccaggacaat
     4621 aaccttatag cttgtaaatt ctatcataat tgtggtttca aaatcggctc cgtcgatact
     4681 atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt
     4741 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     4801 tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg
     4861 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     4921 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     4981 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     5041 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     5101 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     5161 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     5221 attgtcccta tacgaatagc ttagacagcc gattagccga attggattac ttactgaata
     5281 acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc
     5341 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     5401 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     5461 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
     5521 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     5581 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagatt
     5641 tagatgtcta aaaagcttta actacaagct ttttagacat ctaatctttt ctgaagtaca
     5701 tccgcaactg tccatactct gatgttttat atcttttcta aaagttcgct agatagagtt
     5761 ctatattaga gatgtaaaga gttttggtga agaggtttgt gagtatggct tttataacca
     5821 gggtgcaatg agaagcacta acagcagcta gcttaagaac gcataggata tactttttat
     5881 ggaagtcaca gaggaggaat aaaatttgaa caggattaac agagtaacct ctattcttat
     5941 tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt gcacagcgat ttaatataag
     6001 cttaaggaca gtttacagag atatccggac acttgaagaa gccggaatac caattggatc
     6061 tgaggccgga aaaggatatt ttcttgtaga gggcttccta cttccgccgg taatgtttac
     6121 agcggcggaa gtgggtgcat tgattacagc agggaaattt ttaaattgcc atggagatga
     6181 atcatttata aaggattttg attcagctat gtataaaatc aaatctattt tgaagcatgg
     6241 tgaaaaaaac tatgcacagg agttggagaa cagtattaac gtgtacagca catctggaca
     6301 gaaaaataca ttggcggata acgttattgc agcaattcag actgcaatct gcaataaaag
     6361 ggtaatatca attcaatatc ctgcatcagg agggcaagaa ccggaaagca gaatgataga
     6421 gccggttctg ttgcaaagtt ttaaataaag aataaaatcc c
//
LOCUS       DAXWLH010000405         5606 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.155, whole genome shotgun sequence.
ACCESSION   DAXWLH010000405 DAXWLH010000000
VERSION     DAXWLH010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.155"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            241..1209
                     /locus_tag="ACGE36_003016"
     CDS             241..1209
                     /locus_tag="ACGE36_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8385299.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE36_003017"
     CDS             1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE36_003017"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8385300.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2239..2847
                     /locus_tag="ACGE36_003018"
     CDS             2239..2847
                     /locus_tag="ACGE36_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8385301.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE36_003019"
     CDS             3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE36_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8385302.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE36_003020"
     CDS             4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE36_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8385303.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5111..5440)
                     /locus_tag="ACGE36_003021"
     CDS             complement(5111..5440)
                     /locus_tag="ACGE36_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8385304.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT         1787 a          968 c         1201 g         1650 t
ORIGIN      
        1 tggtgtcatc caccaacatc aaaaagtata gagccataat tttttaggaa aatctcaagg
       61 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
      121 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
      181 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
      241 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
      301 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
      361 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
      421 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
      481 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
      541 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
      601 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
      661 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
      721 gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
      781 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
      841 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
      901 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
      961 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
     1021 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
     1081 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
     1141 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
     1201 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
     1261 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
     1321 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
     1381 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
     1441 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
     1501 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
     1561 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
     1621 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
     1681 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
     1741 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
     1801 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
     1861 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
     1921 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1981 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     2041 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     2101 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     2161 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     2221 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     2281 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     2341 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     2401 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     2461 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     2521 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     2581 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     2641 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     2701 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     2761 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     2821 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     2881 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     2941 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     3001 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     3061 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     3121 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     3181 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     3241 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     3301 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     3361 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     3421 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     3481 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     3541 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     3601 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     3661 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     3721 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     3781 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     3841 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     3901 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     3961 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     4021 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     4081 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     4141 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     4201 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     4261 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     4321 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     4381 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     4441 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     4501 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     4561 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     4621 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     4681 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     4741 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     4801 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     4861 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     4921 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca
     4981 aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta
     5041 acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat agatagattt
     5101 tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct aaagagtaat
     5161 aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa aaacgtaaat
     5221 ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag acgcacattt
     5281 ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg aaacatttcc
     5341 ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt ccatttactt
     5401 tttctttatg tacgacagtc acttggcaaa cttttttcat tcatctcacc tcacattcaa
     5461 acgattattt gaatgaagta tacctatttg atcactcttt tgtcaatcta ccttaattcc
     5521 aatacactat tataagcaat ttcatgtatt gaagtaaact tacttgcaag tgcgtttttg
     5581 attccaagtt aaaaacatat tattat
//
LOCUS       DAXWLH010000406         4555 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.187, whole genome shotgun sequence.
ACCESSION   DAXWLH010000406 DAXWLH010000000
VERSION     DAXWLH010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.187"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            446..910
                     /locus_tag="ACGE36_003022"
     CDS             446..910
                     /locus_tag="ACGE36_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8385305.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            894..1523
                     /locus_tag="ACGE36_003023"
     CDS             894..1523
                     /locus_tag="ACGE36_003023"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8385306.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1877..3355
                     /gene="msr(C)"
                     /locus_tag="ACGE36_003024"
     CDS             1877..3355
                     /gene="msr(C)"
                     /locus_tag="ACGE36_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8385307.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3402..3848
                     /locus_tag="ACGE36_003025"
     CDS             3402..3848
                     /locus_tag="ACGE36_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8385308.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4047..>4555)
                     /locus_tag="ACGE36_003026"
     CDS             complement(4047..>4555)
                     /locus_tag="ACGE36_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8385309.1"
                     /translation="LAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKLDQQ
                     GYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLDKTT
                     ATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQVR
                     EQMQQFFE"
BASE COUNT         1473 a          764 c          931 g         1387 t
ORIGIN      
        1 acagccaaac aaaaacagat tttcatgttt tttgtttggt tgaacttatt accgcagatg
       61 caagtttttg aacaccgttt actcaaaaaa ggttgtgaca acatcttgtc ataacttttt
      121 tattactgtt taagcatact gatccaattt ctaatattcg acgggattct tgcaaaaggc
      181 gatttcggaa atttttggcg gtgtaaaatt ttctactgca gaagagctat cttctatagt
      241 ttgataagaa tcaataatct tggttttagc gttgtgcttt ctttatcatt ttttccgttc
      301 attctacaaa aaatttcttt tctctatact tttctattga gagcgttttt tttattacac
      361 agctgattct ttgacttttt tcttttttta ttctattatt gacaagtcaa tcgttgacga
      421 gtcaattatt agaaaggagg aaattatgtc tttagaagag attagtcacc ttttatatca
      481 aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa gaaaatatcg gtataagtct
      541 tactagatac gagatcatga tgagactgaa agaagaaaat ccattgctac aaagcagttt
      601 gcaagaaaag ttgcagattg atcaaggagc agttactcgt catctgaagg tattggaagc
      661 aaatggctat gtttcgcgtc agagaaatcc tcagaacaac cgggaagtgt atgtagcgct
      721 aacagaaaaa gcccagaaga aaatcgagca ttgtgaaatg gaccataaag atacaggttc
      781 cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa tttctaacac ttttagataa
      841 attgaatacc caactttgca atataacaac agaaacgaaa gaggttaata ataatggaac
      901 aaaaaaatga tttttcaaca attatgactg gtagaagatc cgttcgcgta tatgatgaaa
      961 catacaaaat cccacacgaa gaaatgctgg aaatgatcaa agaagcgacg actgcacctt
     1021 cttctgtcaa tatgcaacca tggcgctttg tggtcgctga aagcgaggaa gcaaaagaaa
     1081 tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga tacttcttct gcaatggtca
     1141 tgatttttgg cgatatgcaa tgttacgaat atggcgaaga aatctataac caagcttacg
     1201 aatcagggaa gatgccaaaa gaagtcagag atcaacaatt agctgcgatc attccttatt
     1261 acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa agtcgattct agcttagcgg
     1321 ctatgcaatt catgttagtt gcgcgtgatc atggctatga aacaaatcca attggtggat
     1381 tcgaagcaga tcagttggca gaggcatttg gtttggataa agaccgttat gttccagtta
     1441 ttattttatc aataggaaaa gcggtagaag aaggttacga gtctgttcgt ttagcaccag
     1501 aaaagatcac tacgttcgaa taaaaggttt tcagttacta ttcagtagct attgcttttt
     1561 ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg catcgatgaa
     1621 attacgtttc gaacttttga ataacaacta aataaattca aaagacatgt ataaacaaca
     1681 atttcatgga tggagtatgt ccattttttc gaacgacagt agaataggtg aaagctatga
     1741 cagatttggt gatttagtca tagttatttc gttcagagta ggaaaagatg tgatgcagtt
     1801 tttttgcaaa cagatttttc tgctcttttt tagtatcgat aattgttgtt ttacatgaaa
     1861 taggagtgta aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt
     1921 tggcaaccag ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg
     1981 gatcatagga gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact
     2041 ttttcccgat catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac
     2101 ctatctcgct gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact
     2161 ggaagaaact tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg
     2221 tcttctttct tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag
     2281 aaaaagtatt gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt
     2341 tagtcatgat cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg
     2401 agaaatccga gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa
     2461 aaagactcag ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc
     2521 gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa
     2581 gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc
     2641 catccaaaag aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa
     2701 gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg
     2761 ctatcccatc atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa
     2821 tggtgatttt cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg
     2881 taagacaacc ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa
     2941 agtaagcttt caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat
     3001 tcgttttgtc atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca
     3061 cttagggttt gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc
     3121 gacccgtctg acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga
     3181 accgactaat tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata
     3241 tccgggaacg attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga
     3301 agtttatgaa ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaatgtg
     3361 ctacgatgat tttcgtgaca aatttaaagg agtggtacag attgcctaca aataaaaaag
     3421 aaggaataat ttttacaaca atgatgtgtt ttttgatggt cttggggatg agcgcgtata
     3481 atttatggct gcatcaagat cttcatttat caagtttgtt gattggtcta gtgcctggat
     3541 tcgtggtggc tttcattttg gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct
     3601 ttaaactacc agtaaatcca gaaagcaagg tccaaatgat cttaactatt tcatgcttga
     3661 tggttttagg aatggtgaca tgtatgtctt tattcggtgt gatcatggaa caaggaatac
     3721 ctgatcaatt attctctgct tatatgaatg cttggaaaat gaatgtgatt gccgctttac
     3781 cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca attcaaagta
     3841 atgaatgatg gaacactgtt atttgaaaaa aggccttggc atgctttttg tcatagtctc
     3901 ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg ttttggcctg tcgttcggat
     3961 cgctttttct tttccaactg tattgctctt ttattgaaga taatttgctc ttcaatagat
     4021 atttctagaa aggttctgtt tatcccttac tcaaaaaact gctgcatttg ttctcttact
     4081 tgctgacacc attggagttc ctgctgacaa agcataattc tgcgctggat cacaacataa
     4141 cgtccaaatt cttgtttctt gtctagcgag aggtcattca attttttttg atttttttgc
     4201 atgattttgt tcagctgttg ttctctagtg aatagcagtc cagtagctgt tgttttatcg
     4261 agcgtcgaaa aagcatagat ttttgctaga aattcatctt tttgcgtagg agcgggagtt
     4321 tcctctttga tccattcctc tacaagagca cgtccttcat cagtcaagtg gtagatcttt
     4381 ttttgagagt gctcgtcttt gtccaaaacg gtcacataac cctgctggtc taacttggca
     4441 agcgttgtat agatctgact gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat
     4501 tcatacccag aacgtggttt ggataaaagc acgcataata atatataggc taaag
//
LOCUS       DAXWLH010000407         4471 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.192, whole genome shotgun sequence.
ACCESSION   DAXWLH010000407 DAXWLH010000000
VERSION     DAXWLH010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.192"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            108..191
                     /locus_tag="ACGE36_003027"
     CDS             108..191
                     /locus_tag="ACGE36_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8385310.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            316..1053
                     /gene="erm(B)"
                     /locus_tag="ACGE36_003028"
     CDS             316..1053
                     /gene="erm(B)"
                     /locus_tag="ACGE36_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8385311.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1223..1483
                     /locus_tag="ACGE36_003029"
                     /pseudo
     CDS             1223..1483
                     /locus_tag="ACGE36_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1646..2311
                     /locus_tag="ACGE36_003030"
                     /pseudo
     CDS             1646..2311
                     /locus_tag="ACGE36_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2347..2592
                     /locus_tag="ACGE36_003031"
     CDS             2347..2592
                     /locus_tag="ACGE36_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8385312.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2695..3564
                     /locus_tag="ACGE36_003032"
     CDS             2695..3564
                     /locus_tag="ACGE36_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8385313.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3545..4279
                     /locus_tag="ACGE36_003033"
     CDS             3545..4279
                     /locus_tag="ACGE36_003033"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8385314.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4312..>4471
                     /locus_tag="ACGE36_003034"
     CDS             4312..>4471
                     /locus_tag="ACGE36_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFF8385315.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIE"
BASE COUNT         1605 a          656 c          867 g         1343 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattt tgtgtataat aggaattgaa
       61 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
      121 aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
      181 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      241 agataactaa aattacaaac aaatcgttta acttctgtat ttatttacag atgtaataac
      301 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      361 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      421 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      481 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacatt
      541 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      601 aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      661 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      721 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
      781 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
      841 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
      901 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
      961 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     1021 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt ttaaatttgg
     1081 aaagttacac gttactaaag ggaatggaga taaattatta gatatactac tgacagcttc
     1141 caagaagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag gggtacaaat
     1201 tcccactaag cgctcgggac cccttgtagg aaaatgtcct aagtgtggca acaatattgt
     1261 attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa tgtaagttta ctttagctga
     1321 acattttaga aagaaaaaac taaccaaaac gaatgtaaaa gaattactgg agggaaaaga
     1381 aaccctggta aaaggaatca aaaacaaaga gaaaaagccc tacaatgccg ttgtaaaaat
     1441 tggggaaaag ggatatattg attttatctc tttttcaaaa taaacatggg gctgacccaa
     1501 aagtagctcc tatatgaaaa atagaggtct ttccatttag atagtagtgg aaagacctct
     1561 atttatcttt tgaaagtgaa tacaaaaata aagcacactc tgctataata taggtgatta
     1621 atccatttga gagaggtgct ttatcatgta tcaaaattat accataaacc aactttgttt
     1681 gcctatagat cttgaaatta aattagaaga aaatgatttt gctcatgcca ttgtgcagtt
     1741 tgttgactct attcccgatg aagtctttct tccttattat caatctatgg gtagacccca
     1801 atatcatcct cgtatgctac tttccattat tctttgtgcc tatattcaag gggtttattc
     1861 gggccgccaa atacaaaata tgctgataga tagtattcgg atgcgctact tatctcaaga
     1921 gcaattccct aactttcgta ctattaatcg ttttcgcgtt catccgatta tgaacgatat
     1981 tctagatcat tcatttgttc aatttcgtga gctactggtt caaagtggct taatcagtgg
     2041 cagtgctcta tacattgatg gtacaaaaat tgaagcggat gcgaataaat actctttcgt
     2101 ttggaaaaaa agtattttaa agtataagga tagccttgat caaaaagccc ttgaaaacta
     2161 ccaacaaatg gtggaaacaa aaatcttacc tgagttaata gatgatttaa aggatgaact
     2221 ttctatagaa gagattgaaa ggattcgtca atctttggaa gaaaaggaaa atcaattaat
     2281 tgaagcgatt gaccaaaccg aaacagtaga agggggctga cccattatta ataaggagtg
     2341 gacaacatga ttgatgtaaa tgagctattg gaagaagcta ttagagaaac tgaaaatcta
     2401 aatgatggcg aagtttttct tgttaaagat ttattcaagg gatatgtggg gaataggata
     2461 cccagaaagg atcgacttct gcttggaacg ttatttttaa accatgttag caaaatgaat
     2521 ggtaatttaa aggctattga aaagacttca tctaatcagc agaggtataa aaagacaatt
     2581 gataagtttt aactatcttt tgtgaaatta aaaaaattat aaatatgttg agtagaagca
     2641 aaagatttgg cttggaattt ttacagcagt gaatcgataa aaggagcatg cttgatggta
     2701 gataatatta ttaaatcagt agcagagaaa ttatcctctc tgtcttatat agaaggtatt
     2761 gttttaggtg gttcacgtgc aaggggcacc catacagagg attcggatat agatatcggc
     2821 atctattaca attcagaatc atttgacata aatactatta atcaattcgc tacaaagctg
     2881 gatgatgagc atagaaataa ccttgttgta cctcccggag catggggtga ttggattaat
     2941 ggcggcggat ggttagtcat aaacgggtat catgtggatt taattttacg tgatattaaa
     3001 cgtgtggaac aaataatgaa agatacagag cacggaattg ttactgccaa ttatcagact
     3061 gggcatcccc atggttatat tagtgcaatg tatcgaggag aattagcgat tagcaaaata
     3121 ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac aggcagaaac ttatcccaat
     3181 gctttgcaga aaagtttagt taactttttt atgtttgaag cagggttctc tttaatgttt
     3241 gtaaaagcaa attcgggaac agacgataaa tattatattg cgggtcatgt ttttcgtata
     3301 gtttcatgtt taaatcaagt gttatttgca tgtaataatg cttattgtat caacgaaaag
     3361 aaagctataa aactgcttga aacttttgaa cataaacctg aaaaatatac cgagaaggta
     3421 aatcatattt ttgaagtact cggtatctca ctttttgaat gctacgacat gaccgagaag
     3481 ctttataatg aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     3541 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     3601 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     3661 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     3721 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     3781 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     3841 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     3901 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     3961 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     4021 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     4081 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     4141 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     4201 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     4261 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     4321 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     4381 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     4441 gatattacat attttgtaag tgatatagaa c
//
LOCUS       DAXWLH010000408         2147 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.264, whole genome shotgun sequence.
ACCESSION   DAXWLH010000408 DAXWLH010000000
VERSION     DAXWLH010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.264"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            265..960
                     /gene="vanR-A"
                     /locus_tag="ACGE36_003035"
     CDS             265..960
                     /gene="vanR-A"
                     /locus_tag="ACGE36_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8385316.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            938..2092
                     /locus_tag="ACGE36_003036"
     CDS             938..2092
                     /locus_tag="ACGE36_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8385317.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          717 a          399 c          459 g          572 t
ORIGIN      
        1 aatgtatcta gggcttcatt atacaggaaa ttatcagaag tgaataatta gccattctgt
       61 attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct
      121 cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca tcatcttaag aaattcttag
      181 tcatttatta tgtaaatgct tataaattcg gccctataat ctgataaatt attaagggca
      241 aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga tgatgaacat
      301 gaaattgccg atttggttga attatactta aaaaacgaga attatacggt tttcaaatac
      361 tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct tgccatattg
      421 gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag ggacaagcac
      481 acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat tacagggtta
      541 acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt aattgctcgg
      601 gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca gaacgaaaat
      661 gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta tctgaacgag
      721 aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg tgaaaacaag
      781 gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga atatttcagc
      841 aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc
      901 attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa
      961 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     1021 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     1081 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     1141 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     1201 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     1261 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     1321 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     1381 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     1441 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     1501 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     1561 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     1621 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     1681 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     1741 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     1801 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa
     1861 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     1921 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     1981 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     2041 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     2101 tataattttg gctctttgtc aaatgatgtt ggtaaagaga aatctta
//
LOCUS       DAXWLH010000409         1527 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.308, whole genome shotgun sequence.
ACCESSION   DAXWLH010000409 DAXWLH010000000
VERSION     DAXWLH010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.308"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..385
                     /locus_tag="ACGE36_003037"
     CDS             <1..385
                     /locus_tag="ACGE36_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8385318.1"
                     /translation="PADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYN
                     DAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTT
                     GEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            483..>1527
                     /gene="tet"
                     /locus_tag="ACGE36_003038"
     CDS             483..>1527
                     /gene="tet"
                     /locus_tag="ACGE36_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8385319.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTELGRSRPSSI"
BASE COUNT          454 a          231 c          305 g          537 t
ORIGIN      
        1 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
       61 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
      121 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
      181 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
      241 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
      301 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
      361 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
      421 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
      481 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
      541 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
      601 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
      661 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
      721 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
      781 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
      841 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
      901 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
      961 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     1021 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     1081 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     1141 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     1201 agaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     1261 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     1321 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     1381 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     1441 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaacagaat
     1501 taggcagaag tcgcccatcc tctatag
//
LOCUS       DAXWLH010000410          944 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.359, whole genome shotgun sequence.
ACCESSION   DAXWLH010000410 DAXWLH010000000
VERSION     DAXWLH010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.359"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            <1..815
                     /locus_tag="ACGE36_003039"
                     /note="aph(2'')-Ia"
     CDS             <1..815
                     /locus_tag="ACGE36_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFF8385320.1"
                     /translation="SGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLET
                     NVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHG
                     LDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKC
                     LCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILR
                     MYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          415 a           60 c          157 g          312 t
ORIGIN      
        1 gtagtggtta tgatagtgtg gcatatttag ttaataatga atacattttt aaaacaaaat
       61 ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc aatatataat tttttaaata
      121 caaatttaga aactaatgta aaaattccta atattgaata ttcgtatatt agtgatgaat
      181 tatctatact aggttataaa gaaattaaag gaactttttt aacaccagaa atttattcta
      241 ctatgtcaga agaagaacaa aatttgttaa aacgagatat tgccagtttt ttaagacaaa
      301 tgcacggttt agattataca gatattagtg aatgtactat tgataataaa caaaatgtat
      361 tagaagagta tatattgttg cgtgaaacta tttataatga tttaactgat atagaaaaag
      421 attatataga aagttttatg gaaagactaa atgcaacaac agtttttgag ggtaaaaagt
      481 gtttatgcca taatgatttt agttgtaatc atctattgtt agatggcaat aatagattaa
      541 ctggaataat tgattttgga gattctggaa ttatagatga atattgtgat tttatatact
      601 tacttgaaga tagtgaagaa gaaataggaa caaattttgg agaagatata ttaagaatgt
      661 atggaaatat agatattgag aaagcaaaag aatatcaaga tatagttgaa gaatattatc
      721 ctattgaaac tattgtttat ggaattaaaa atattaaaca ggaatttatc gaaaatggta
      781 gaaaagaaat ttataaaagg acttataaag attgattata taatatatga aaagctatta
      841 taaaagacat tagtattaaa tagtttaaaa aaatgaaaaa taataaagga agtgagtcaa
      901 ttccaggaga gtgtaaaata ttttgtgtaa atagaaaaaa ggaa
//
LOCUS       DAXWLH010000411          858 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.367, whole genome shotgun sequence.
ACCESSION   DAXWLH010000411 DAXWLH010000000
VERSION     DAXWLH010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.367"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            255..803
                     /gene="aac(6')"
                     /locus_tag="ACGE36_003040"
     CDS             255..803
                     /gene="aac(6')"
                     /locus_tag="ACGE36_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8385321.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          305 a          126 c          182 g          245 t
ORIGIN      
        1 attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt
       61 tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat
      121 aattttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
      181 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
      241 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
      301 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
      361 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
      421 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
      481 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
      541 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
      601 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
      661 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
      721 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
      781 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
      841 aataagaagg aatttacg
//
LOCUS       DAXWLH010000412          711 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.381, whole genome shotgun sequence.
ACCESSION   DAXWLH010000412 DAXWLH010000000
VERSION     DAXWLH010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.381"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            123..620
                     /gene="dfrG"
                     /locus_tag="ACGE36_003041"
     CDS             123..620
                     /gene="dfrG"
                     /locus_tag="ACGE36_003041"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8385322.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          273 a           74 c          147 g          217 t
ORIGIN      
        1 ttatgataat ataacttata gaaaaaattg cttattcaac taacgggcag gataatgaga
       61 aaaggttttt tgatagaaat aagaaattag atggtaaatt agttaaaaag gctgtgttat
      121 aaatgaaagt ttctttgatt gctgcgatgg ataagaatag agtgattggc aaagagaatg
      181 acattccttg gaggattccc aaggactggg aatatgttaa aaatactaca aagggacatc
      241 cgataatatt aggtaggaag aaccttgaat caatcggaag agccttacct gacagaagaa
      301 atattattct gacgagagat aaggggttta cctttaatgg ttgtgaaatt gttcattcaa
      361 tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat ttttattttc ggaggagaac
      421 agatttataa tttgtttttc ccttatgttg agaaaatgta catcacaaaa atacatcatg
      481 aattcgaagg agatactttt tttccagaag tgaattatga ggaatggaat gaggtatttg
      541 cccaaaaagg gataaagaat gataaaaatc cgtataacta ctattttcat gtatatgaaa
      601 gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac
      661 aagagcttgg gattactcca agctcttttt gtcatttgaa ataaattata t
//
LOCUS       DAXWLH010000413          536 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id9
            SAMN40749885-rid21412673.guided.398, whole genome shotgun sequence.
ACCESSION   DAXWLH010000413 DAXWLH010000000
VERSION     DAXWLH010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749885
            Sequence Read Archive: SRR28555111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134721.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:39:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,888
            CDSs (with protein)               :: 2,888
            Genes (RNA)                       :: 56
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 49 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 14 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749885-rid21412673.guided.398"
                     /isolate="id9"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2021-12-22"
     gene            149..498
                     /locus_tag="ACGE36_003042"
                     /pseudo
     CDS             149..498
                     /locus_tag="ACGE36_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          176 a           93 c           73 g          194 t
ORIGIN      
        1 attagtaaca agatcagttc tttgttattc gttatttttc tattatgttt gttcattttt
       61 atactccttc ttatatttcc taatagagta taacatagct ttaagcatta cttaaagata
      121 acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat
      181 ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac
      241 ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt
      301 gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt
      361 ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac
      421 gactatgata atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc
      481 tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatc
//
